LOCUS       DAZSNO010000001        78080 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZSNO010000001 DAZSNO010000000
VERSION     DAZSNO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78080
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.001"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHWN2_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHWN2_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR8929728.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHWN2_000002"
     CDS             complement(1672..2667)
                     /locus_tag="ACHWN2_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8929729.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHWN2_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHWN2_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHWN2_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHWN2_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR8929730.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHWN2_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHWN2_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR8929731.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHWN2_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHWN2_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR8929732.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACHWN2_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACHWN2_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR8929733.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACHWN2_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACHWN2_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR8929734.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHWN2_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHWN2_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR8929735.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHWN2_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHWN2_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR8929736.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHWN2_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHWN2_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR8929737.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHWN2_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHWN2_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR8929738.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="ACHWN2_000013"
     CDS             complement(17699..18322)
                     /locus_tag="ACHWN2_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR8929739.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHWN2_000014"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHWN2_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR8929740.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="ACHWN2_000015"
     CDS             complement(18971..19213)
                     /locus_tag="ACHWN2_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR8929741.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHWN2_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHWN2_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR8929742.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="ACHWN2_000017"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="ACHWN2_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR8929743.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="ACHWN2_000018"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="ACHWN2_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR8929744.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="ACHWN2_000019"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="ACHWN2_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="ACHWN2_000020"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="ACHWN2_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR8929745.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="ACHWN2_000021"
                     /pseudo
     CDS             24840..25697
                     /locus_tag="ACHWN2_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="ACHWN2_000022"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="ACHWN2_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR8929746.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="ACHWN2_000023"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="ACHWN2_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR8929747.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..29673)
                     /locus_tag="ACHWN2_000024"
     CDS             complement(29257..29673)
                     /locus_tag="ACHWN2_000024"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFR8929748.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(29670..29777)
                     /locus_tag="ACHWN2_000025"
     CDS             complement(29670..29777)
                     /locus_tag="ACHWN2_000025"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8929749.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            complement(29738..30340)
                     /gene="ypdF"
                     /locus_tag="ACHWN2_000026"
                     /pseudo
     CDS             complement(29738..30340)
                     /gene="ypdF"
                     /locus_tag="ACHWN2_000026"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="ACHWN2_000027"
     CDS             complement(30355..31602)
                     /locus_tag="ACHWN2_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR8929750.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="ACHWN2_000028"
     CDS             complement(31624..31950)
                     /locus_tag="ACHWN2_000028"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR8929751.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="ACHWN2_000029"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="ACHWN2_000029"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR8929752.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="ACHWN2_000030"
     CDS             33338..34594
                     /locus_tag="ACHWN2_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR8929753.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /gene="ypeC"
                     /locus_tag="ACHWN2_000031"
     CDS             34709..35035
                     /gene="ypeC"
                     /locus_tag="ACHWN2_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR8929754.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /locus_tag="ACHWN2_000032"
     CDS             complement(35176..36414)
                     /locus_tag="ACHWN2_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR8929755.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="ACHWN2_000033"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="ACHWN2_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR8929756.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="ACHWN2_000034"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="ACHWN2_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(40322..40397)
                     /locus_tag="ACHWN2_000035"
     tRNA            complement(40322..40397)
                     /locus_tag="ACHWN2_000035"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40362..40364),aa:Ala,seq:ggc)
     gene            complement(40437..40512)
                     /locus_tag="ACHWN2_000036"
     tRNA            complement(40437..40512)
                     /locus_tag="ACHWN2_000036"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40477..40479),aa:Ala,seq:ggc)
     gene            40733..41092
                     /locus_tag="ACHWN2_000037"
     CDS             40733..41092
                     /locus_tag="ACHWN2_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR8929757.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41094..41486
                     /locus_tag="ACHWN2_000038"
     CDS             41094..41486
                     /locus_tag="ACHWN2_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR8929758.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="ACHWN2_000039"
     CDS             complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="ACHWN2_000039"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR8929759.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43212..43287
                     /locus_tag="ACHWN2_000040"
     tRNA            43212..43287
                     /locus_tag="ACHWN2_000040"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43245..43247,aa:Val,seq:tac)
     gene            43332..43407
                     /locus_tag="ACHWN2_000041"
     tRNA            43332..43407
                     /locus_tag="ACHWN2_000041"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43365..43367,aa:Val,seq:tac)
     gene            43412..43487
                     /locus_tag="ACHWN2_000042"
     tRNA            43412..43487
                     /locus_tag="ACHWN2_000042"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43445..43447,aa:Lys,seq:ttt)
     gene            43518..43643
                     /locus_tag="ACHWN2_000043"
     ncRNA           43518..43643
                     /ncRNA_class="other"
                     /locus_tag="ACHWN2_000043"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43763..44104
                     /locus_tag="ACHWN2_000044"
     CDS             43763..44104
                     /locus_tag="ACHWN2_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR8929760.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44095..45021)
                     /locus_tag="ACHWN2_000045"
     CDS             complement(44095..45021)
                     /locus_tag="ACHWN2_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8929761.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45111..46109
                     /locus_tag="ACHWN2_000046"
     CDS             45111..46109
                     /locus_tag="ACHWN2_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR8929762.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLVHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46106..46324)
                     /locus_tag="ACHWN2_000047"
     CDS             complement(46106..46324)
                     /locus_tag="ACHWN2_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR8929763.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46326..48341)
                     /gene="ligA"
                     /locus_tag="ACHWN2_000048"
     CDS             complement(46326..48341)
                     /gene="ligA"
                     /locus_tag="ACHWN2_000048"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR8929764.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48412..49398)
                     /gene="zipA"
                     /locus_tag="ACHWN2_000049"
     CDS             complement(48412..49398)
                     /gene="zipA"
                     /locus_tag="ACHWN2_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR8929765.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49628..50389
                     /gene="cysZ"
                     /locus_tag="ACHWN2_000050"
     CDS             49628..50389
                     /gene="cysZ"
                     /locus_tag="ACHWN2_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR8929766.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50574..51545
                     /gene="cysK"
                     /locus_tag="ACHWN2_000051"
     CDS             50574..51545
                     /gene="cysK"
                     /locus_tag="ACHWN2_000051"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR8929767.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51929..52186
                     /gene="ptsH"
                     /locus_tag="ACHWN2_000052"
     CDS             51929..52186
                     /gene="ptsH"
                     /locus_tag="ACHWN2_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR8929768.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52231..53958
                     /gene="ptsI"
                     /locus_tag="ACHWN2_000053"
     CDS             52231..53958
                     /gene="ptsI"
                     /locus_tag="ACHWN2_000053"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR8929769.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53999..54508
                     /gene="crr"
                     /locus_tag="ACHWN2_000054"
     CDS             53999..54508
                     /gene="crr"
                     /locus_tag="ACHWN2_000054"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR8929770.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54550..55401)
                     /gene="pdxK"
                     /locus_tag="ACHWN2_000055"
                     /gene_synonym="thiJ"
     CDS             complement(54550..55401)
                     /gene="pdxK"
                     /locus_tag="ACHWN2_000055"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR8929771.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55506..55880
                     /locus_tag="ACHWN2_000056"
     CDS             55506..55880
                     /locus_tag="ACHWN2_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR8929772.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55913..56636
                     /locus_tag="ACHWN2_000057"
                     /pseudo
     CDS             55913..56636
                     /locus_tag="ACHWN2_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56840..57751)
                     /gene="cysM"
                     /locus_tag="ACHWN2_000058"
     CDS             complement(56840..57751)
                     /gene="cysM"
                     /locus_tag="ACHWN2_000058"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR8929773.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57885..58982)
                     /gene="cysA"
                     /locus_tag="ACHWN2_000059"
     CDS             complement(57885..58982)
                     /gene="cysA"
                     /locus_tag="ACHWN2_000059"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR8929774.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58972..59847)
                     /gene="cysW"
                     /locus_tag="ACHWN2_000060"
     CDS             complement(58972..59847)
                     /gene="cysW"
                     /locus_tag="ACHWN2_000060"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR8929775.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59847..60680)
                     /gene="cysT"
                     /locus_tag="ACHWN2_000061"
     CDS             complement(59847..60680)
                     /gene="cysT"
                     /locus_tag="ACHWN2_000061"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR8929776.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60680..61696)
                     /gene="cysP"
                     /locus_tag="ACHWN2_000062"
     CDS             complement(60680..61696)
                     /gene="cysP"
                     /locus_tag="ACHWN2_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR8929777.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62000..62791)
                     /gene="ucpA"
                     /locus_tag="ACHWN2_000063"
     CDS             complement(62000..62791)
                     /gene="ucpA"
                     /locus_tag="ACHWN2_000063"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR8929778.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62920..63778)
                     /gene="murR"
                     /locus_tag="ACHWN2_000064"
                     /pseudo
     CDS             complement(62920..63778)
                     /gene="murR"
                     /locus_tag="ACHWN2_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            63942..64838
                     /gene="murQ"
                     /locus_tag="ACHWN2_000065"
     CDS             63942..64838
                     /gene="murQ"
                     /locus_tag="ACHWN2_000065"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR8929779.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64842..66266
                     /gene="murP"
                     /locus_tag="ACHWN2_000066"
     CDS             64842..66266
                     /gene="murP"
                     /locus_tag="ACHWN2_000066"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFR8929780.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            66271..66357
                     /locus_tag="ACHWN2_000067"
                     /pseudo
     CDS             66271..66357
                     /locus_tag="ACHWN2_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66446..67345)
                     /gene="yfeX"
                     /locus_tag="ACHWN2_000068"
     CDS             complement(66446..67345)
                     /gene="yfeX"
                     /locus_tag="ACHWN2_000068"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR8929781.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67441..68016)
                     /locus_tag="ACHWN2_000069"
     CDS             complement(67441..68016)
                     /locus_tag="ACHWN2_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR8929782.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68077..68526)
                     /locus_tag="ACHWN2_000070"
     CDS             complement(68077..68526)
                     /locus_tag="ACHWN2_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR8929783.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68513..68938)
                     /locus_tag="ACHWN2_000071"
     CDS             complement(68513..68938)
                     /locus_tag="ACHWN2_000071"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR8929784.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69152..70021
                     /gene="amiA"
                     /locus_tag="ACHWN2_000072"
     CDS             69152..70021
                     /gene="amiA"
                     /locus_tag="ACHWN2_000072"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR8929785.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70025..70924
                     /gene="hemF"
                     /locus_tag="ACHWN2_000073"
     CDS             70025..70924
                     /gene="hemF"
                     /locus_tag="ACHWN2_000073"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR8929786.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70930..71982)
                     /gene="eutR"
                     /locus_tag="ACHWN2_000074"
                     /pseudo
     CDS             complement(70930..71982)
                     /gene="eutR"
                     /locus_tag="ACHWN2_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72028..72528)
                     /gene="eutK"
                     /locus_tag="ACHWN2_000075"
     CDS             complement(72028..72528)
                     /gene="eutK"
                     /locus_tag="ACHWN2_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR8929787.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDIQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72541..73200)
                     /gene="eutL"
                     /locus_tag="ACHWN2_000076"
     CDS             complement(72541..73200)
                     /gene="eutL"
                     /locus_tag="ACHWN2_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR8929788.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(73210..73278)
                     /locus_tag="ACHWN2_000077"
                     /pseudo
     CDS             complement(73210..73278)
                     /locus_tag="ACHWN2_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            74382..75173
                     /locus_tag="ACHWN2_000078"
     CDS             74382..75173
                     /locus_tag="ACHWN2_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8929789.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75893..76288
                     /locus_tag="ACHWN2_000079"
     CDS             75893..76288
                     /locus_tag="ACHWN2_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR8929790.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76306..76569
                     /locus_tag="ACHWN2_000080"
     CDS             76306..76569
                     /locus_tag="ACHWN2_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8929791.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76592..77134
                     /locus_tag="ACHWN2_000081"
     CDS             76592..77134
                     /locus_tag="ACHWN2_000081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8929792.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77273..77560
                     /locus_tag="ACHWN2_000082"
     CDS             77273..77560
                     /locus_tag="ACHWN2_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8929793.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77599..77805
                     /locus_tag="ACHWN2_000083"
     CDS             77599..77805
                     /locus_tag="ACHWN2_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8929794.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
BASE COUNT        20253 a        19951 c        18609 g        19267 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcat aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcacgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga caaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 ttgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaagt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagccaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgact
    31981 agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct agacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatctta cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctgatt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaagcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
    40321 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc
    40381 agctgagcta tagccccacg atgcgtttac gtaccaaatt tgctgggtgc aaaatttggt
    40441 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
    40501 agctatagcc ccgtcacgta aagcttgtcg agttgacggg cggcatcata tgaattccgc
    40561 ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat cgctgaaaaa tcaggcaaat
    40621 gaacaatttt ggaaaaccgc tcgcattcag tagttattca tgtcacggtt tcctgtaaag
    40681 tggtgttata aaatgaacta ctaatagacc cacatacatt cagggaattg ttatgttcaa
    40741 ggagaggatg acaccagatg aacttgccag actgaccggc tatagccgcc agaccattaa
    40801 taaatgggta cgcaaggaag gctggacaac gtcaccaaaa cctggcgtcc agggtggcaa
    40861 agcccgactg gttcacgtca atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc
    40921 agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg ccgcttgaag tattgctggt
    40981 gacactggcg aaagagatga cgccagttga acaaaaacag tttacatcct tgcttctgcg
    41041 ggaagggatt atcggattgt tacaacgctt agggattcgc gatagcaaat aatatgaaaa
    41101 gattacgcaa taaaatgacc accgaagaac tggctgaatg cctcggtgtg gctaaacaaa
    41161 ctgttaaccg ttggaccaga gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag
    41221 gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg cgagtttatt cagaacacgc
    41281 cagccttcca taacacacca atgttgatgg aagctgaaga gagaattgct gaatacgctc
    41341 ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc gatagataat atgacagaca
    41401 ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg aattcgcaat ttcctcgccc
    41461 gtctcgatat tgacgaatca gcataaataa aaaacggcag gatattatct cctgccgttt
    41521 atctttttac acgctaatta ctgctgattt tcgcgttcag caataaaatc cagcgctttg
    41581 ttgatacgct cgatactgcg ggtcttacca attgcgtgaa cggtaacatc cagtgctgga
    41641 gactgccccg caccggttac ggcgacacgc agcggcatac caactttacc catacccact
    41701 tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta
    41761 atcgcggcca gtttgtcacg aaccacttcc agcggctgac gcgctaccgg acgcagatgt
    41821 tttttcgccg catcggcatc gaactcagca aaatcttcgt agaaataacg gcagctctgt
    41881 gccatctctt tcagcgtctt gcagcgttcg cccagcagtt tcaccagatc agccagctgc
    41941 gggccgttac gggtatcgat attttcctgc tcaatgtgcc actgtaagtg agtagcaaca
    42001 tactccggcg gcagcgcgtt aatgtagtga tggttcagcc acagcagctt gtcggtgttg
    42061 aacgcactgg cagatttgct gacggcattc aaagtgaagt atttgatcat ctcttcacga
    42121 gtgaagattt cctgatcgcc gtgggaccag cccagacgca ccagatagtt cagcagtgct
    42181 tctggcaaat aaccgtcatc acgatactgc attacgctga ctgccccgtg acgtttggac
    42241 agttttttac cgtcatcgcc attgatcata gaaacgtgcg cgtaaaccgg caccggcgct
    42301 ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt tgatatggtc ttcgccacgg
    42361 ataacgtggg tgatttccat atcccagtca tcgacaacca cacagaagtt ataggttggg
    42421 gaaccatcgg tacggcggat aataagatcg tccagttcct ggttgctgaa ctcgatcgga
    42481 ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg ggttagcaaa acgtacaaca
    42541 cacggttcat catcagcatg atgctcatgg ctgtggcggc agcgaccgtc ataacgcggc
    42601 ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag
    42661 catttataag cagtgccctc ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa
    42721 cgtttggtct ggtagtacgg accttcatcc cactccaggc tcagccagtt catgccatcc
    42781 ataatggctt cgatagcttc cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc
    42841 acgaactcac cgccgtggtt acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg
    42901 ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag ttttgatttt catgaaatgg
    42961 ccttacgttt agaaagatgc cgacaaccgg caaatcctgg aaaaattgag tgggcgatat
    43021 tctatcactc ccgcctgatt cctcaatgta gttcgggttt ctaccttacg cattattgtt
    43081 ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca tcttgcttaa atttacgacg
    43141 aacgaacaat ttctttagaa aatgcgttga ctcattttga actctcccta taatgcgact
    43201 ccacacagcg ggggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43261 gcggttcgat cccgtcatca cccaccaact actttatgta gtctccgccg tgtagcaaga
    43321 aattgagaag tgggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43381 gcggttcgat cccgtcatca cccaccactt cgggtcgtta gctcagttgg tagagcagtt
    43441 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatg taaaaaagcg
    43501 ccctaaaggc gcttttttgc tatctgcgat actcaaagat tcgaacctgc agcaggtttg
    43561 agttgagcgc agcgaaacaa cggagccgct cgcggcgacg gcccgaaggg cgagcgaagc
    43621 gagtcatcct gcacgaccca ccaatgcaaa aaagcgccct aaaggcgctt ttttgctatc
    43681 tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc
    43741 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
    43801 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
    43861 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
    43921 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
    43981 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
    44041 gtctctgaat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
    44101 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
    44161 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
    44221 ctggcgttca accaccggcg taatgcgctt aaccaccagt gggctacctt caggcaatgg
    44281 tagcgccagt gcaggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
    44341 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
    44401 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
    44461 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
    44521 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
    44581 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
    44641 atgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
    44701 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
    44761 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
    44821 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
    44881 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
    44941 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
    45001 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
    45061 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
    45121 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
    45181 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
    45241 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
    45301 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
    45361 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
    45421 acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
    45481 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
    45541 caccattgct ggttggtctg gtgatgaatg ttcacggtgc aaggggcagc cttgagcagg
    45601 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggtgcatctt tcccggccgt
    45661 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
    45721 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
    45781 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
    45841 ttgtagttaa cgtctttatg gcacgccgac tgagcttcaa taaggcagat gaaattacta
    45901 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
    45961 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
    46021 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
    46081 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
    46141 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
    46201 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtca attaagcgac
    46261 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
    46321 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
    46381 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
    46441 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
    46501 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
    46561 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
    46621 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
    46681 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
    46741 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
    46801 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
    46861 tgtgccaatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
    46921 agatctgccg gagtatggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
    46981 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
    47041 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
    47101 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
    47161 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
    47221 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
    47281 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
    47341 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
    47401 ttaaacgcaa ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
    47461 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
    47521 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
    47581 ctgaccggca ccccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
    47641 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
    47701 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
    47761 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
    47821 acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaatg
    47881 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
    47941 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
    48001 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
    48061 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
    48121 cgtatctggc tgaaagctgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
    48181 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
    48241 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcaaagc
    48301 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
    48361 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
    48421 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
    48481 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
    48541 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
    48601 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
    48661 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
    48721 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
    48781 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
    48841 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
    48901 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
    48961 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
    49021 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
    49081 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt aggtactggt gttgcggcga
    49141 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
    49201 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
    49261 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
    49321 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
    49381 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
    49441 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
    49501 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
    49561 ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat
    49621 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
    49681 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
    49741 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
    49801 cccgactttc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
    49861 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
    49921 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
    49981 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
    50041 cgaatggcaa aaatttgtct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
    50101 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
    50161 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
    50221 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
    50281 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac
    50341 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
    50401 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
    50461 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
    50521 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
    50581 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
    50641 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
    50701 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
    50761 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
    50821 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
    50881 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
    50941 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
    51001 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
    51061 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
    51121 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
    51181 ttgagccaac cgattctcca gttatcgccc aggtgctggc aggtgaagag attaaacctg
    51241 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
    51301 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
    51361 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcagcgttga
    51421 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
    51481 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
    51541 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
    51601 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
    51661 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
    51721 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
    51781 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa
    51841 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
    51901 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc
    51961 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
    52021 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
    52081 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
    52141 gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
    52201 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
    52261 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
    52321 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
    52381 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
    52441 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
    52501 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
    52561 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
    52621 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
    52681 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
    52741 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
    52801 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
    52861 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
    52921 ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa
    52981 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
    53041 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
    53101 ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa
    53161 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
    53221 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
    53281 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
    53341 cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
    53401 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
    53461 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
    53521 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
    53581 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
    53641 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    53701 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    53761 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    53821 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    53881 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    53941 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    54001 gggtttgttc gataaactga aatctctggt ttccgacgac aaaaaggata ccggaactat
    54061 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    54121 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    54181 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    54241 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    54301 cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat
    54361 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    54421 ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga
    54481 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg
    54541 cgccattttt tatgcttccg ccagcggcgg caaaatcaat tcatcgctct catgctgctg
    54601 ggtgtagcgc atcacttcca gtacgcgcaa ccccgcacgg tgcaccgcat cgtttaacgc
    54661 cttccctttc agcaaaccac tgataagctg agcacaaaac aggtcgccag tccctttcag
    54721 gtcggttttt acccgtgaat gggaaatgac attcacgctg tcggcactga ccaccaccac
    54781 ctgcatctcc tgattttctt cattaccgga ggcgctggta atcaccaccc attttaatgt
    54841 gtctgaaagc agactttttg cggcagcaat ggcactgtcg agatcgcggc aatttttacc
    54901 ggtcaggatt tccagctcaa agatattggg ggtaattccc tgcgccagcg gcagtaaata
    54961 ttgtcgatac gcctcgggaa ggtcaggttt gacataaatt ccgctatcaa tatcgccaat
    55021 caccggatcg accatgatca ataggtcagg atggtctttg cgtagcgcag tcagccactc
    55081 ggcaaggatt ttgatttgcg atgccgttcc catatagccc gtggttacag cacgaagttg
    55141 gcgcagcgca tcacgctcct gaagcgcacg caaatagccg ctaaaccatt cgtccggaat
    55201 cgcaccaccg tagaaagtgt cataatgcgg cgtattgctc agcaataccg tcggcacggc
    55261 aaagacattc aagccgttct gttttatagc aggcaccgca atgctgttgc ctacgcttcc
    55321 gtacactacc tgcgactgca cggcgacgat atccgcctgc agcgccctac tcttatcgtt
    55381 aaacaacaac aaactactca tttaattttt tctccttgcc gatgatcctc atcgtattcc
    55441 aaccgaaact ttacctgatt ctggcagtca aatcggctat tacaaaacaa agataaggta
    55501 attcagtgaa gaaaatcatt tgtctggcca ttacgctgct aatgacactc cccgcttacg
    55561 cgaagttaac tgttcatgaa gaagcccgca tcaacgccat gctggaggga ttagcacaga
    55621 aaaaggattt gatatttgtg cgcaacggtg atgaacatac ctgcgatgaa gcggtttccc
    55681 acctgcgtct gaagctcggc aatacccgta accgcattga cactgccgag cagtttattg
    55741 ataaggtagc ttcgtcgtca tcgattaccg ggaagccgta tatcgtgaag atccccggta
    55801 agagcgatga gaacgcacag ccttttttac atgcgttaat tgcgcagacg gataaaacgg
    55861 tgcctgcgga aggaaattaa tccgctttgg taaggcattt acaggaggta acatgaaaaa
    55921 atgctttatt tatcacgatg aaaaatcgaa taaattttgg tggatagatt acgaagggga
    55981 tagtttagct gtcaactatg gcaaggtagg tagtattggt aaattccata caaaagaatt
    56041 cgataatgaa gaacagtgtc tgaaagaagc cagtaaattg attgccgcaa aaatgaaaaa
    56101 aggatatcaa gaaactccag actttaactt catggatctc tactattttg atgatgaaga
    56161 gattgggtta catcttaaaa cgtcacaccc aaacttccag tgccatttta ctgatccact
    56221 ttatatgtgt tgctgggatg aagaatctcc ttttggtagc gatgaaggtg ctgatgctct
    56281 aaacgaactt gaaaatagcc tccgttctgg actgtgctga tttccctcaa atgttaattg
    56341 aaactatgtg gggtatgaaa tacatcgcta tggacagtat tcttgaagag gatgtgcgtg
    56401 cgcaattact agccgatgaa atgagctcta tccagagcaa tatgattacc tacgcgactg
    56461 cattcggtca gatcaaagtc atgggtaaaa tctcccataa acttaaaaag atgggactca
    56521 atgcactagc gcgtcatcag cttaccgcaa aaattcttca atggggtgac ggtcaggact
    56581 caccaatact tcaaaaaatg attgatgacc ttactgcttt tccacacgaa aattaaaaac
    56641 tgcatttgtc ggcagcaaca actgccgaca aatcagatta ttgacaaagt gcactttgtt
    56701 tatgccggat gcggcgtaaa cgcctgatcc ggcataaaaa tattgctaat tcaatatatt
    56761 gcagaaacat tgtaggcctg ataagcggag cgcatcaggc agttttgcgt ttgtcatcag
    56821 tctccaatgc tattaatcct taaatccctg ccccctgact aaaatgctct tccccaaaca
    56881 ctccggtgga gaggtagcga tcgccacgat cgcagatgat cgctaccacc accgcaccag
    56941 ggttagcttt tgccacccgc agtgctccag caaccgcgcc gcctgagctg acgccacaga
    57001 atattccttc ccgcaccgcc agttcgcgca tggtgttttc cgcatcgcgc tgatgaatat
    57061 ccagcacctc atccaccagc gcagcgttga aaatccccgg cagatattcc gcaggccagc
    57121 ggcgaatgcc cggaatactg ctgccttctt ccggttgcag gccaacaatg gtcaccggtt
    57181 tggattgttc gcgcataaag catgaaacgc cggtgatagt gccggtcgtc cccatgctgg
    57241 agacaaaatg agtgatgcgc ccgccggttt gctgccagat ttccggcccg gtggtggtgt
    57301 aatgcgcata agggttatcg ggattattga actgatcgag cagctttcct tcgccacgat
    57361 tcgccatctc cagcgccaga tcgcgcgcac cttccatgcc ctgctctttg gtgacaagaa
    57421 tcagttccgc accataagca cgcatcgccg cacggcgttc ctggctcatg ttgtcgggca
    57481 tcagcaattt catgcgatag cctttcagcg cggcaatcat tgccagcgca atgccggtgt
    57541 taccactggt ggcttcgatt aagacatcac ccggtttaat ttccccgcgc ttttccgcct
    57601 cgacgatcat cgaaagtgcc gcacgatctt tcaccgaacc tgccgggtta ttgccttcca
    57661 gttttaacca cacttcactg ccgttatccg gccccattcg ctgcaacttc accagaggcg
    57721 tattgcctat tgtttgttct aatgtactca caatctctat ccatacgtgg tgttgcctga
    57781 tgcgacgctt gcgcgtctta tcaggcctac aggttacaaa ccttgccatt aaaaagcccg
    57841 gatcgcggga agcgcctccg ggcgtttaac attcactcaa cctatcaggc gctttgtgcg
    57901 agagcaagtt cctcatcgcg ggtttcaata cgctcgtcgc cgttatacag ccgcgcatgt
    57961 tgcagaccaa cgaataaacg ctcgccatgc tgcggggcat cgtcgccatg catcacgacc
    58021 gtcagcggtt cgttgtacca ccccagcggc tgcaccacta attgggtgta gtgacctttc
    58081 gggctggctt ccagtacctg taccggcagc ggcgaatcga gactggtacg gcggctgata
    58141 tccacttccc acgggcgcag gaagagatcc accggcccct gatacgcagg tgtgtagccc
    58201 agcggccagc gatgcgcgcc aacatggaac tgcccgccgc gaatggttcc ctgcaggcgg
    58261 ttcacttcgc ccataaattc gagtacaaaa cgggtcgctg gttcgcgcca tacctgattc
    58321 ggcgcatcag cctgttcaat attcccctga ctcatcacca ctacacgatc ggctacttcg
    58381 gtcgcctctt cctggtcgtg ggtcacaaac acgctggtga atttcagctc ttcatgcagt
    58441 tgacgcagcc agcgacgcag ctctttacgc acctgcgcat ccagcgcgcc gaacggttca
    58501 tcaagcagca gaatttgcgg ttctacagcc agcgcgcgcg ccagcgccac acgctgtttc
    58561 tggccgccgg aaagctgcgc cggataacga tccgccagat gcgcaagctg gaccatttcc
    58621 agcaattttg tcactttcgc tttgatggct gcggcattcg ggcgctcgcg acgcggcagc
    58681 accgtcaggc caaaagtgat attgtcgaat accgtcatat ggcggaacag cgcgtaatgc
    58741 tggaacacga aaccgacttt acgatcgcgc gcgtgcaggc gactcacgtc ggtgccgtgg
    58801 aagcgaatat gcccgctggt ttgatgctcc agcccggcga taatccgcag cagcgtggtt
    58861 ttcccggaac cggacggccc cagcaacgcg accatctgac cagaaggaat atccagtgag
    58921 atatcgttca gcacctgggt gcgaccaaaa gacttcttga tattggcaat ctcaatgctc
    58981 atgatgctcc tcctgctgcg cgcgtttttc ctgattctcc aggcgccact gcaacatact
    59041 ctttaaaaac agggtgataa tcgccatcaa cgtcaacagc gcggcggcgg taaaggagcc
    59101 gacggtgttg tagtcctgct ccaacaattc aatctgtaac ggcagcgaaa gtgtttcgcc
    59161 gcgaatcgag ccggaaacca ccgacactgc accaaactcg ccaatcgcgc gggcgttggt
    59221 cagcaccacg ccgtaaagca gcgcccagcg aatattcggc aatgtgacgc gacggaacat
    59281 ctgccagccg gacgcgccaa gcaaaatcgc cgcttcgtct tcctggctgc cctggcttaa
    59341 catcaccggc accagctcgc gcaccacaaa cggacacgtc acaaagatgg tgaccagcac
    59401 cattccgggc caggagaaca taatttgcag attatgttca tccagccagc cgccgagcgg
    59461 gccgttggag ccgtagaaca gcagatacac cagaccggca accaccggcg atacagcaaa
    59521 cggaatgtcc agtagcgtca gcagtaactg gcgtcccggg aagttaaagc gcgtcaccag
    59581 ccaggccagc agaatgccga acaccaggtt taccggtacg gcaatcagcg cgatcatcac
    59641 cgtcagccag atggcgtgca gcatgtccgg atcggccaga ttctgtaaaa ccggcatcag
    59701 ccccttgctg aatgcctgca cgaagatgta aatcatcggc accagcagga tgaacgccga
    59761 aaccagcatc ccgatgccaa tcagaaacca tttgccccag ttaatcgggc gcgcgtcata
    59821 acgcttcaat tgggtaactt ccgccattaa tgacctacca cacgccgacc aaagcgactt
    59881 tgcagagtgt taattgagaa cagcagcagc agagatgccg cgaggatcac cgaagcaatc
    59941 gcgctcgctg ccgggtaatc aaactcctgt aagcgcacaa aaatcatcag cgacgtcact
    60001 tccgtcttcc acgcgatatt tccggcgata aaaatcaccg cgccaaattc accaagacta
    60061 cgggtaaacg acagcgccac gcccgccacc agcgccggag aaagctccgg cagcaccact
    60121 ttgcagaaac tctgccagcg cgttgcacca agcgtttccg ccgcttcttc atattccggg
    60181 cctaactctt ccagcaccgg ctgcacggta cgcaccacaa acggaatgct ggtaaaggcc
    60241 atcgccaccg caataccaag ccaggtatag gtgactttga tatcaaactt cgccagccat
    60301 tcaccgtaaa aaccgtttac ggaaaagagc gaggccagcg ttaaaccggc gactgccgtt
    60361 ggcagcgcaa agggtaaatc catcagcgca tcaagcagcg tgcggcccgg aaagcgataa
    60421 cgtgttaaaa tccacgccat cagcagaccg aaaacgccgt taaaaatcga tgccacaaac
    60481 gccgacagca gcgtcacttt gtaggccgcg accacctgcg ggttggtgat cacctcccag
    60541 tactgcgccc agctcatctc agaaagctgc atcaccagcg cagagagcgg cagcagcaat
    60601 atcaggcaca caaacagcag actggtgcca aggcttaaag taaagcccgg cagcacgcgt
    60661 cttgaagaaa ctgcaaacat tacttacgcc ccgccgctaa cagcttgtct aactcgccgc
    60721 cgctggtgaa gtgggttttc atcacttccg gccatgagcc aaatttgtct tccacgcgga
    60781 acagttcggt ctgcgggaat ttatctttca gtttgtccat gacttccggg ttattcacac
    60841 ggtagtaata gtcggtgatg atggtctgcg cctgtgggct gtagagccag ttcaggtagg
    60901 cttttgcggc tttttccgta ccgttggcct gcacattttt atcgacccac gccaccggga
    60961 attccgccag aatattggtt ttcgggatca ccacttcaaa gccctgtgct tcatactgtt
    61021 tacggatgtt gttcacttcc gattcaaagc taatcagcac atcgcccaag ccacgctcgg
    61081 caaaagtggt ggttgcgcca cgaccgccgg tatcgaacac ttcaacgttt ttcaggaact
    61141 gggtcataaa ctgttcggtt ttggctttat cgccaccgtc agctttatcc gctgcgcccc
    61201 atgccgccag ataggtataa cgcgcgttac ccgacgtttt cgggttcggg aaaatcagct
    61261 tcacgtcgga gcgcaccagg tcgttccagt cgtggatatt cttcgggttg cccttacgca
    61321 ccaggaagcc catggtggag tagaacggcg agctattatt cggcaggcgc gactgccagt
    61381 cagccgggat cagcttgcct ttgtcgtgca ggatttgtac gtcggtcacc tggttataag
    61441 tgacaacgtc tgcttttaag ccctgcaaaa tcgccagcgc ctgttttgat gacccggcat
    61501 gagattgttt tatcgtcagt ttgtcgccgc cgttatcttt tgcccattgc tgttcaaacg
    61561 gcggattcag ggcggcaaac agctcgcggg agacgtcata agaactgttc agcagttccg
    61621 ttgcctgtac atggcccgcc agcagcagag aagcgaccag cgcgagtgag ttctttttca
    61681 gtaagttaac ggccattgcg cacccttata aatttaatga ctttctaata gccatcatat
    61741 ttataacgga cgtgaaagga gtaacggttt tatataccgt ttggtgattt ggaagttaaa
    61801 aagggaataa gaacttggtt caacaggcgt aattgttgca ggcagtttgg acacggacag
    61861 cgcggagaaa ccggagcgta cacatagtac gtgaggattt cgagcactgc ccggggccaa
    61921 aatgacaaat gaaatagcct ggtgaactta gttcaagacg aaatcctccc cacacatgca
    61981 gggaggaaac agaggtgaat cagataccga cgctaaccgt ctccggcagt gtgctgccgc
    62041 catcaatcac attctgtgta ccggttaaat agctggattc atccgatgcg aggaaggccg
    62101 ccagttcgcc gacttccagc ggatcggcga ggcgacacag cgggattgct tttgccattt
    62161 cagtcagcac cgattctgga tcttccgggt tcgactggcg ggcaatgctt tccgccattg
    62221 gcgtgcggac gtatcccggg cagatggcgt taacgcgaat accggattgc gcgtactcca
    62281 ccgccagcga tttagtcagg ccaacaatcg ccgctttcgt taaggcatac gccgtttcgc
    62341 caggatcggc caccatatca ccagtgactg aagacatcat cacaatgcga ccatctttgc
    62401 gcgcaatcat ctccggcagc accgccttcg tgacgttcca tacgccttta atattgatat
    62461 caatatggaa atcgcgatct tcatcgctca tatcgaggaa actgcccaga cgacaaacgc
    62521 ctgcgttatt caccaggata tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg
    62581 cggctaccga cgccgggtca cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac
    62641 cacacagttc gtccgctagc ttttcgatct caggggagat atccagcaag attaggttcg
    62701 cgccatgacg tgcaaaagtt ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa
    62761 tcagtgctgt cttgcccgtg agtttaccca ttttaatgct ctccttgtta atacagagcg
    62821 ttacactcca ttccctctga aaatacagga taaacaaaat attgctgcgg atcagctcac
    62881 taattcagga aaatctcaat ccagtcgctg ctttatgcat catttcaggc gttgtgttag
    62941 ctcactgctg cgctgaatca ttttcaatga cgccacatca ttgatgttgc acaagaccaa
    63001 caaacagtaa atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc
    63061 attcggtttc accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt
    63121 cagccagaga agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca
    63181 agacaatctc ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca
    63241 acgcctggga aacggtggct tgtacgtgcg tgtcggcttc gcaggccacg cgatagccaa
    63301 ttttcatcag tttgaacgac agatcgcgcc ccaccagcgc cgatccgccc aggcccgtta
    63361 tctggataaa cggtgcttta ctgatgacct caatgatttt ttgcagccgc gcgtagtcga
    63421 acaacgcaca ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca
    63481 cttccagcga atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt
    63541 tctcccggct ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg
    63601 cgccgagctt ctgggcaaat ttcacaatgc tcgactggct gataccgagc tgtttcgcca
    63661 tctgccgtga agagacggat ttcagctcgc tcactctggc ccgcagaaag tcagcaattt
    63721 tttgctcgtt cccggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct
    63781 tccactctcc tgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact
    63841 ctgcgagtgt agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata
    63901 catctgcaat tatttgatcg taaatagtaa ggtcaccacc gatgcaacta gaaaagatga
    63961 ttactgaagg ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt
    64021 gccggattat caacgatgaa gataaaaccg taccgcctgc cgttgatcgc gtcctgccgg
    64081 atatcgccgc ggcgatcgat gttatccacg cccaggtcag cggcggcggt cgtctgattt
    64141 atctcggtgc gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca
    64201 cctacggcgt gaaaccgggt ctggtggttg gtttgattgc tggaggcgaa tatgccattc
    64261 agcacgcggt ggaaggagct gaagatagcc gggaaggtgg tgttaacgat ctgaaaaata
    64321 ttaatttaac ggcacaggat gtggtggttg gcattgctgc cagcggtcgc acgccgtatg
    64381 tgattgccgg actggaatac gcacgccagc tcggctgccg cacagtggga atttcctgta
    64441 atccggggag cgccgtttca accaccgctg agtttgccat tacaccgatt gtaggtgccg
    64501 aagttgttac cggttcttcg cggatgaaag caggtacagc gcagaaactg gtgctcaata
    64561 tgctttccac cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg
    64621 tggtcgccac caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg
    64681 gatgtaatgc cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg
    64741 ccattgtgat ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac
    64801 acggcggctt tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca
    64861 gtgaacttct gaacaccatt cttacccgtg tcggcggacc gggaaatatc gccagttgtg
    64921 gtaactgtat gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata
    64981 tcaaaacgct ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt
    65041 ttgggcctgg taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgatgcac
    65101 cggtacagga tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa
    65161 cctccggcgt gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg
    65221 gttttattgc cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg
    65281 ttccggcaga tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca
    65341 aaggtttgtt caccttccta gtgattctgg tgggctataa cgccgcccag gcatttggcg
    65401 gcacgggcgt caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg
    65461 caaccaccgg ttactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg
    65521 gcaatattat cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc
    65581 gccgctttat gccggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca
    65641 ccgctacgct tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt
    65701 catggctgtt tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt
    65761 tcctgatcgc cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa
    65821 tggacagcca gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg
    65881 tgggcgcggc gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg
    65941 tacgcggggc gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca
    66001 ccctgccccg catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt
    66061 ttatcggatt gatagcctgg tggggtctgc cgatgggctt aaacagcgcc tttgggccgt
    66121 ctggtctggt ggcgctgccg ctgatgactt ccgcacaagg catccttccg gcaatggcgg
    66181 tttatgctgg cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg
    66241 gctgccgtaa cgtcaatctg gactgatatt atgaaacgga caatgctcta tctttctctg
    66301 ctggctgtta gttgtagcgt cagcgccgca aaataccctg ttctgacaga aggtagttga
    66361 tcaagtatat gcggcgttaa agcagtaata aaaaggccag tcagtaatgt tactgcctgg
    66421 cctttaatca atgaatcaga aacgcttaca gcgccatcaa cttgtccagc gacggtgcga
    66481 aataatagcc gccggttacc ggtttggtga aacgcaacat cgcatcacgt ttgccatcgg
    66541 tatcgccaaa catgctcagc agttgctgct caatgttatg cagacgcgcg cagtaggcgc
    66601 agaagtacag accgtgagtg ccactggcgg tgccgtacgg caggctctgg cgaacaatct
    66661 tcagcccttt gccatcttct ttcagatcaa cgcgggtgag gtgagaggtt tccggacgtt
    66721 cgtcgccgtc aatctcttcg ttggcctctt tggtgcgccc gatcatcatc tcctgatcgt
    66781 gaacgctcat ccggttgagc tgcttcaggt tgtgctccca acgctgcaca aacacatagc
    66841 tgccgccgga atccacgccg tctttgataa ccgccacttc gcggcgcgtc tcttcacccg
    66901 ccgggttttc tgtaccgtca acaaagccgc tcaggtcacg ctcttccacc caacggaagc
    66961 cgtggatctc ttctttcact tcaatgcagt caccaaaggc ttccatcgcc gcctgagcga
    67021 cagagaagtt tacgtcgtga cgcagagaaa gaatgtggat caacacatcg aactgggtcg
    67081 tcggcgcaag gcctttaccg tagcccggaa aatctttcag ctcttctgcc ccaacgccgc
    67141 cgctcagagc gcgccaggtg ttgttaccaa aggcaaccac cgcaccaaga tgcgcgtccg
    67201 ggaatttcgc ttcaaaagtt gccagtttgt cggcaaatgt tttactggcc gcacgcaggg
    67261 cgtcaacttc ccctttcacg ttggcttcga tccaaatcgc cgcgcggcaa tgttctggca
    67321 aaatgccact ctgaacctga gacattgttc ctcctgaaaa taagaatgcc acgcaatccg
    67381 tggcattgtt gagcgctatt ttacgtgttt tttcgcgacg gcggtttgtt cagacgcaaa
    67441 ttaacgccgc cagataattt tactgacttt ccagtttttc agggtatcgt ccgaaggcat
    67501 taacccttca ggaccacgcc attccccgct gaactggtag ctaatatgtt gactgccttc
    67561 ggctttacat tcgacggcac gattatcatc accatcagct ttttggcaat tgccaaatgc
    67621 tttgctgtaa aggtcgctaa acggtgtacc gattttaaca ccggtgtcag caggaatatc
    67681 gctatccagc acgtcaatgc gactgatcgt gccttgatcg ccgttaatca ccatcgccac
    67741 gttgtcgcct ttcatcactt caaaaaagcg caccacgttg ccgttcgtgg ttttcattcc
    67801 gctgcgcagg cgataatcgc catcaagcgc atcagcaatg gcttgttctt gcagtggtgt
    67861 ggacgccgtt aattcaccca cgccctgctc gctcactttg gtggacgatc caaaccagtt
    67921 ccacgggtta gcggcagacc agttaactga cgacatcgtg gaacagccgg tcagcatcaa
    67981 cggcatagcg cataacatta aacgcagcga tttcatgtca cttcctttgg ttattcaata
    68041 acgttgcttg gagtgcaaat tcaccaaaaa gtgccgttat tctttttctt cattaaaaca
    68101 agcgcgtaaa cgtcgattgg tcagcagcca gattaacgcc acaatatccg ccactaccag
    68161 cgccagaccg ataccgctaa cggattcacc gttcagccac agccacggtt gccagcaaag
    68221 taaaaccacc tgcgccaaca gcaacagaaa atagagcaca tgccaggtgc gaggaaacga
    68281 agcccgccga ccgctcaaca aaaacgccag caccgccgga ataccgggaa tcagccccag
    68341 ccagaaatta tcgtgatcgg gataaaacag atttagcagc gcagtaccct gctcacgcga
    68401 cgcaccggca atgacaaaca gcacccaggt tcgcgcctga agcaatagca caagccagaa
    68461 gagcaagggt aaacgcaggc gtccgtgcgc atcataatgg acaggatgga actcagtact
    68521 cttcatcttc aatcaaacgc ttacccagac tcagcacgtc ggcgtgttca tatcccaggc
    68581 gttcatacat tccgagcacc atgtcgttat cttccggcac gttgatctga attttcgggc
    68641 agccgcgagc aatcagcttt ttctccagcc ggttaagcaa cgcattggca attccacgcc
    68701 cacgaaactc tggatgcacg ccaagataat aagcagaccc gcgatgcccg tcataaccgc
    68761 ccatcaccgt tccgaccact tcaccgttta cctcagcgac caaaaacaaa ctgacgtcat
    68821 ggttcatctt acgctcgatg tccatttccg gatcgttcca cggacgcagc aagtcgcaac
    68881 gctcccaaag ggtgatgacc tcttcgaaat cttcctggcg aaatacgcgt atctccatgg
    68941 tattcgttac ctttttgcgg gttaaaaggc tgattatggc gtgaacggtc gaattagcca
    69001 atatctgacg aaaatcggtt gaaaaagtgg cataatgggg agttgtcaac tattgaaatg
    69061 aaaagtaaaa caattctcaa cagcaaaccg tcgtaacgga ttacgcgata cgatataaca
    69121 tctggaactt tattattaca actcaggccg tatgagcact tttaaaccac taaaaacact
    69181 cacttcgcgc cgccaggtgc tgaaagccgg tttagctgcc ctgacgttgt caggaatgtc
    69241 gcaagccatt gccaaagacg aacctttaaa aaccagcaac ggacacagca agccgaaagc
    69301 caaaaaatct ggcggcaaac gtgtcgttgt tctcgatcca ggccacggcg gaattgatac
    69361 cggagcgatc ggacgcaacg gttcgaaaga aaaacatgtg gtgctggcga ttgctaaaaa
    69421 cgtccgttcc attttgcgta atcatgggat tgatgcgcgt ttaacgcgtt ctggcgatac
    69481 gtttatccca ctttacgatc gcgttgaaat cgcccataaa catggcgcag atctgtttat
    69541 gtcaattcat gccgatggct ttaccaaccc gaaagctgcc ggtgcttcgg tatttgccct
    69601 ctctaaccgt ggggcaagta gcgcaatggc gaaatacctg tctgaacgcg aaaaccgcgc
    69661 cgatgaagtt gccggtaaaa aggcgactga caaggatcac ctattgcagc aagtactgtt
    69721 tgatctggtg caaaccgata ctattaaaaa cagcctgacg cttggctcgc atattctgaa
    69781 aaagattaag ccggtgcata aactgcacag ccgcaacacc gaacaagccg cgtttgtggt
    69841 gttgaaatcg ccgtcggttc cttcggtgct ggtggaaacc tcgtttatca ccaacccgga
    69901 agaagagcgg ctgttaggca cggcggcgtt tcgtcagaaa atcgccacag cgattgctga
    69961 aggcgtgatc agttatttcc actggttcga caaccagaaa gcacattcga aaaagcgata
    70021 agttatgaaa cccgacgcac accaggttaa acagtttctg ctcaaccttc aggatacgat
    70081 ttgtcagcag ctgaccgccg tcgatggcgc agaatttgtc gaagatagtt ggcagcgcga
    70141 agctggcggc ggcgggcgca gtcgggtgtt gcgtaatggt ggtgttttcg aacaggcggg
    70201 cgtcaacttt tctcacgtcc acggtgaggc gatgcctgct tccgccaccg ctcatcgccc
    70261 ggaacttgcc gggcgcagtt tcgaggcgat gggcgtttca ctggtagtgc atccgcataa
    70321 cccgtatgtt cccaccagcc acgcgaatgt gcgttttttt attgccgaaa aaccgggtgc
    70381 agagcccgtt tggtggtttg gcggcggctt cgatttaacc cctttctatg gttttgaaga
    70441 agacgccatt cactggcacc gcaccgcccg tgacctgtgc cagccatttg gtgaagacgt
    70501 ttatccccgt tacaaaaagt ggtgcgacga ttacttctac ctcaaacatc gcaacgaaca
    70561 gcgcggcatt ggcgggctgt tctttgatga tctgaacacg ccagatttcg accactgttt
    70621 tgcctttatg caggcggtag gcaaaggcta caccgacgct tatttaccaa ttgtagagcg
    70681 acgtaaagcg atggcctacg gcgagcgcga gcgcaatttt cagctctacc gtcgcggtcg
    70741 ttatgtcgag ttcaatctgg tctgggatcg cggcacgctg tttggcctgc aaactggcgg
    70801 gcgcaccgag tcgatcctga tgtcaatgcc gccactggta cgctgggagt atgattatca
    70861 gccaaaagat ggcagcccag aagcggcgtt aagtgagttt attaaggtca gggattgggt
    70921 gtaactccct cacccccatt cccgcatccg ctgatgcaac gtcaacgacg gcttctcggc
    70981 aaacagctgc tggtaatccg tggcaaattg ccccagatgc cagaatcccc actgcatggc
    71041 ggcgtctttt accgttgtgc tttgcgacca cgggcttatc agttcgcggc gtacgacgtt
    71101 caggcgaatg cgtttcagcc acgcgtttgg gccaatgcct aaaatagcgt gaaacgcgtt
    71161 ttgtagcgtg cggcgactga catgcagttg attacacaaa tccagcaccg tcaccggttc
    71221 ggacatgttt tccagcacat attcacgggc gcgggaaagc aaccgacggt aactctgatg
    71281 actgatgctt tccgccgtca ccattggttg cgcttcttcc agcatcgccc ccatcgccat
    71341 tagcaaatta tcccccagca cttttcgcac tgcaggctga tggagatttt ccgggttctc
    71401 gcaaaacgtc gccagcgcct gttggacaaa gccccacagc gcggctttat gctgctcttt
    71461 cacttccagc gccgactggc tacgcagcat atgtaatacc cgatccggat tgtgcaaaaa
    71521 gttagcctga caggtgatga catcttcaga cagcactacg cccaggatcg tataatcgtc
    71581 cggcgtactt aattcaaact cggttccacc ggggcgcgtc gctatttctg cgcttcccag
    71641 acattgcgaa ccgataaatc cctgctcacc gcgcgtcgcc ggaatgccaa accagaacga
    71701 gttcggccag accaggcacg actgacgcag cgccagaccg gtgtattcac gaaaaacctg
    71761 aatatcatcg agtagaattt ccgtaaattc accatgaaac ttgcccggat gcagctgatc
    71821 gtaaatctgc tgctaggcgg taatcgtcaa agcatgttca tagacatccg ttgtctgtcg
    71881 ttgatgaaca ttatccactt cgaccttcgg cgtgagcttc aggttttcgg gtaagggttc
    71941 atgataaaga tggtgcaaat tggctgtacg ggtctttttc atgatgttaa tgccgggtgt
    72001 tgtaggacac ccggcacctc cgacaggtta atggggcttg agacgataac gactactgcg
    72061 tttacgtaac gtcccggcag aaaagagctg ttcgagcgca tttctggctt tttccagcgg
    72121 ccagccaaag tgggcggcca cttctcctgc cgtcattccc tgacatactg atgttaacag
    72181 cgccaacaac tcgtcagcag attccgcgac gataactggc gcgtcgggtt cctttacggg
    72241 ttgcttcggc tggcggttaa agccagtaac cagccactgg atgtcatcgt ctggccgacc
    72301 gatctcctta cggctgatga cacgcccggt acgcatcgcg gcagcacaac ctgcgtccag
    72361 cgccgcacga cacgccgcca gatcgccttc caccaccagc gttaagcgac cagggtcaag
    72421 cacttcgtga ctgagcagac gaacgttagc tgctttgagc atggcatccg ctgcatctat
    72481 cgcggcgacc atgccgtcca cttccagcaa tcccagtgca ttgatcatcg gcaacctccg
    72541 ttacgcacgc tggattggat tacgcgcgat ttccagcact gcatcggtaa aggcgttaca
    72601 ggctgcttta cacgcggcct ggctaccggt taaaaatgct gccgagtagt tggtttcaga
    72661 cggtggcggg acgtaggtca ccagctgtac gtcggcagat ttcaacgcgg catcaatgcc
    72721 gtaggtcgct tccagcggcg gtgccaccag atacgccatc ggatcgccaa gcgtgatccc
    72781 ggcggttgat gaaagataag aaccggtacg cgaaaccaca tgcgccagga aggcggtgtt
    72841 ttctgcgtca ttggcccact ggaaagctgc gccattttca atatgtgcaa ccatcgcatc
    72901 cagaccggca cgcacttccg ccgggtttgg cccaccaagc ataatcagca cttcaccggc
    72961 agtcggtgac gggccgtgtg ccgctcccgc atacagcgag cgaccatata ccacttcgac
    73021 catcgcttgt ttggtcgctt cgtcagccgc aatgtaggtt acgtcgtcag aatcggcaga
    73081 aatcagcccg aggctacgaa tatgcggcgg caattttagt tcacgcgcaa attcagcgtt
    73141 aacggaggca atcacccgca tggcggttac cgacggtcga atcaaatcta aagctggcat
    73201 gatgcctcct tatcgggtca tgttgatgcc ggatgctttc tgctccagca tacgtttcgc
    73261 caaatccaca atgacggcac agcagcacga aagagaagca aaagtcagct acctggtaca
    73321 ctaggcagcg ggtcagcaaa ggggggcaaa tgcccctttt ttcatgtcag ataatcagat
    73381 cattacctca ataaactaat aatcacatcg cggttacaac cagtttgata gtgatagctc
    73441 aaacccagac gtggcgctgg ctgcaagcaa aataagccgc aaaaatgcga ccccatgttt
    73501 actagccgca aaaatgcggt cctatttgcc agaacctttt attattcaat tggttagtgc
    73561 aagaaaaagc gtgattgctg ttcaagaagt atccagaaag gtcatgattt tgacgctgaa
    73621 aaaaaatagg tgggcatttt tgcccactca ctagccgcaa atttgcccac tcactagccg
    73681 catttttgcg gcccaaagta ataaatacag agaaataata aatacagatc agcgccaaga
    73741 tttttcgtct tttcggcgga atcatgagac tagtgcttac gtcctgataa cctccagagc
    73801 agaccttaac ttctgtgcag caggactacg ctcattgccg ctcggctagg tcaaatcctc
    73861 acgataaagc tgttcggatt gtgaccataa tgttaaagga ctctttaaat cattccttga
    73921 cgaattaact agctggctta agcggaatag catgcaggat aaaaacaaaa ccagcttagc
    73981 agtggggaga gcttcgcttc gctcgtctcc tggggccaat gctcaatgaa acgacgtaag
    74041 gcacggagcg ctcaccatag gtagacacat aaacatctga gagcgcacct tctatcgcgc
    74101 tacgcgctca ataaaaacca acgacaccgc cacactgcaa gaccaatggt ttttatcgcg
    74161 tatacagacc agacaggaaa cagcagcaaa gcagcgtata gccaggtgca gacagcacac
    74221 tcacacgacg cacagcaggc ttaagtgatc actcaggaaa ctttatcccc gccgccgatc
    74281 accgcgaact atcatgaccg cgccaaattc gaaaggaatg gcccatagaa cgcgatgaac
    74341 acacataagc gctacactta cttaacgtcg ccacagagcg attgagagcg cttaaaatcg
    74401 atgcgatttc attaaatttt tctcactcgc ctgaatggtg gcgatcagga ctgatcgcaa
    74461 cattgccccg cctcgatatt gagcgcataa tagatacata cattaagcgg aaaagagata
    74521 agtttattgt gaaaatcagg gggttacata tcatgcgcat atcagaacaa caacggcaga
    74581 tcctcgacta tctggcaaaa aacgaatcaa tcaccgcacg acaagccgca gatctcgttt
    74641 atggtggcaa tgttacccgc gtccaggttg acgccgcccg ccgctcactc atgacgatgg
    74701 ttagtaatgg gctaatcagg aaacaaggcc gcgctttcgt agctgaaaga catatcaatc
    74761 acgcttatag ccaggacgtt gaagaattgc gccgccagat cctttgggta ttcagcaaac
    74821 agggtcagga gtatttcctt tctgaacacc caaatttcgc cgccaaagag ttaacagtaa
    74881 gcggattgtc gtgcgcactg gattatcttg tcggtgaagg ttgcgttact tacaagcagc
    74941 tatggcacgc cctgaatacg cttgtagaag atggtttact cattgccacg cgtgagaaca
    75001 tcgaatactg gataatgccg cgttcaggtc gccgcaatca tgcacggtat atttcaggca
    75061 acagatacag aacgccggac tttaatccga cacaggaaga tcgggaccgt gccgaaatga
    75121 tgtgcaggga acaggagaaa agaattatgg ataagttatt ttcgatgcag tgaacacaag
    75181 cgggcgcaat gcccgttttt attgccttat tccacgcctg acgaatcaat ttgatcacac
    75241 ctgagaaaat gcatagccag aagttaaggc cgttatattg cgattgggag cgttttaaac
    75301 ggtatcaatt taattaattc tgatgtggtg atttgcaggc aaaaaaaacc cgccggagaa
    75361 gcgggaaata gagacatatc gttgtagtta ttaacagatc actttgaggt cgagggcggc
    75421 taaccctcgc taatattatc ccgctattac cttgcgggtg caaataatct tttattgctt
    75481 tgatgattta ataaatagca ctttttgcta acaaaaaccg ctgtcaatat atggccaaaa
    75541 atgtcaacag aaaaaataag gtttataata ttaagtatca catgggtaat gggttcaggg
    75601 aatcggaatc caccgcctca cggaaattca aaatttgctt cttctttcct ccgtgggcgg
    75661 cttctttttc gtatcgaggt atttatgaaa gcaataacat tagaacagtt cgcagcaatg
    75721 gcgaatgtat cgaccagaac gctgaggcgc tggctaaaaa aaccatcaac cggagtccct
    75781 ttgccattta ccccacgcgg tactcgccaa ttattctggg agcacgaatg caaagaatgg
    75841 ttgtacagaa cagaacgata ataatcacgc atcacatttt gaggtaaaca tcatgaccaa
    75901 tatcgaaaga ttagaacgct gtgccgatat tatgcgacgc agatggattt atgaccctga
    75961 agcgggttta ttgtttagcc gtgaaacaaa agacgttatc aaaggcagca tgaccaataa
    76021 agggcatttg gttgtaacgg tacatgaagg taaattcaaa gtgacactca cctatcagaa
    76081 agcctgctat gtatacacat acggcgctta tgacgagaca ttatatgaag tgcaccacgt
    76141 taatttgaac aaacaggata accgcatcaa gaatattcgc ctgatgccga aagaaaagca
    76201 ccgccggata cataaaaacg tccgcaaact tattcggctg ggccacacac aatggcttca
    76261 accgcaatta attgaacaat cactataaac acaggagtaa ataccatgca ttcattagac
    76321 agttattttc agcgcacgac agcccctaaa tcagccgccc aggaacgccg tgaggaattc
    76381 catgagaaag tgatgcgttc tgctgattat attgcggaca agttcgtgga aacggttcgt
    76441 ccacttgttg atgaagtagc tgataaactt cagtctgaaa tgccggaaga tatggaaggc
    76501 actgcaaagc ggcgactcat ttgcgaatta agtcgtcgtt ttggcgtgtc aataagcgct
    76561 tttaaataat cgaaaaaagg ggggaaagat aatggacgca tttgaggtac tggcaaataa
    76621 aggcgctgaa ctggtagcac agcgtgataa aaccgccaac gaaggggaac gcgcactgct
    76681 aaataaacag attaaagcga tccgtatggc ccagtttaaa ttaatcagta atgaagttat
    76741 cgaaacaatt aaaccgcaga tcccacaaat cgttgctgac gctattaaag ccgccggact
    76801 ggagaaacgc attgccgggc taaaaacgaa cgcagagaaa atggagatgt acaaacagag
    76861 cggtgctccg aatcccgatg agtacttctc accggatgtt gagtttatgg cgcaggttga
    76921 ggaacgtctg ggtagctgtc tcaccgagga acagcgtcgc tattttgatg gagtggatag
    76981 ttcggcaggt attgacctga actcgtattt cggtcgtgaa attgagcatt ttgatgccgc
    77041 cccgtctatg ccggagccag aaccagaaat ggccaccgat cctgaagctg agcaacggaa
    77101 acgcgtattc aatgcgatag ggattcggta ctgatcggat aaaagaaagc cgggaattac
    77161 cccggctgac ttaacaatgt gaccaatgaa caaatcggac aagacaaaca ggataagcag
    77221 tctcgaaaca atgttcggga actatattag cacaagtccg gagaaaatca tcatgttggc
    77281 actacgcatc atgcagggca tcgctaagac cctcgcagaa catgtcttag acctgaaaca
    77341 ttcgccactg agcaaacagg ccatgaagcg ccaaacgctt cgtctgtggg ctgaatattc
    77401 gcttggaact atcaataaaa tcatcgacat gaaatcgggg ccgagcaacc agagcgcgga
    77461 ggaaatggag ttcatcaggc gtttaattct cattcgcaga gacattcata gccagttaca
    77521 cagcgtggga atcgatatta atgatgggac gggcgattaa ttttcggaca agtactatgc
    77581 gcgcgcgagg agctgaacat gaagcaagat attgcagaca ggctggagat actcgaaggc
    77641 cagcgggcag aagcgaaaca actacgtaag caggccagaa gagcgcacag gaacaacgaa
    77701 gcagagttac taaccaaata catcagtttc accaattact gcatctacga gtgttacaag
    77761 gaagatgcag aggactggct ggattcattg ccagaacagt actgagcaga ataacaccac
    77821 gtcatgttgt tctgaggtga gcatatcacc tctgttcagg tggccaaatt tagtgtgcca
    77881 cttcagcatc accgttgaaa tcacaataac gatttttttg taaatatgct ctcacatatg
    77941 taggaataga ccaggttggc attgaaacac atctaccatg accataataa agtaattttt
    78001 tgatatgttt atggttttat attaattaaa atatatctaa atttgatagt tttaaaattg
    78061 caaaatccag attgtgtttg
//
LOCUS       DAZSNO010000002        70712 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZSNO010000002 DAZSNO010000000
VERSION     DAZSNO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70712
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.002"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..795
                     /locus_tag="ACHWN2_000084"
     CDS             <1..795
                     /locus_tag="ACHWN2_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8929795.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="ACHWN2_000085"
     CDS             806..1897
                     /locus_tag="ACHWN2_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8929796.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHWN2_000086"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHWN2_000086"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR8929797.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHWN2_000087"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHWN2_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR8929798.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="ACHWN2_000088"
     CDS             4859..5254
                     /locus_tag="ACHWN2_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR8929799.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="ACHWN2_000089"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="ACHWN2_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR8929800.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="dolP"
                     /locus_tag="ACHWN2_000090"
     CDS             5874..6449
                     /gene="dolP"
                     /locus_tag="ACHWN2_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR8929801.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="ACHWN2_000091"
     CDS             complement(6563..7603)
                     /locus_tag="ACHWN2_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR8929802.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="ACHWN2_000092"
     CDS             complement(7676..8311)
                     /locus_tag="ACHWN2_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR8929803.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHWN2_000093"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHWN2_000093"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR8929804.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="ACHWN2_000094"
     CDS             complement(8937..9380)
                     /locus_tag="ACHWN2_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR8929805.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHWN2_000095"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHWN2_000095"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR8929806.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="ACHWN2_000096"
     CDS             complement(9720..10223)
                     /locus_tag="ACHWN2_000096"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8929807.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="ACHWN2_000097"
     CDS             complement(10217..10741)
                     /locus_tag="ACHWN2_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR8929808.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="ACHWN2_000098"
     CDS             10950..11945
                     /locus_tag="ACHWN2_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR8929809.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="ACHWN2_000099"
     CDS             11954..12832
                     /locus_tag="ACHWN2_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR8929810.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="ACHWN2_000100"
     CDS             12913..13920
                     /locus_tag="ACHWN2_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR8929811.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHWN2_000101"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHWN2_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR8929812.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHWN2_000102"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHWN2_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR8929813.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHWN2_000103"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHWN2_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR8929814.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHWN2_000104"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHWN2_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR8929815.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHWN2_000105"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHWN2_000105"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR8929816.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHWN2_000106"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHWN2_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR8929817.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHWN2_000107"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHWN2_000107"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR8929818.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHWN2_000108"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHWN2_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR8929819.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHWN2_000109"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHWN2_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR8929820.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHWN2_000110"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHWN2_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR8929821.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHWN2_000111"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHWN2_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR8929822.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="ACHWN2_000112"
     tRNA            complement(27654..27730)
                     /locus_tag="ACHWN2_000112"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="ACHWN2_000113"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="ACHWN2_000113"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR8929823.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31055)
                     /locus_tag="ACHWN2_000114"
                     /pseudo
     CDS             complement(29429..31055)
                     /locus_tag="ACHWN2_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(31514..31600)
                     /locus_tag="ACHWN2_000115"
     tRNA            complement(31514..31600)
                     /locus_tag="ACHWN2_000115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHWN2_000116"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHWN2_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR8929824.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHWN2_000117"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHWN2_000117"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR8929825.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHWN2_000118"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHWN2_000118"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR8929826.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHWN2_000119"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHWN2_000119"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR8929827.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHWN2_000120"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHWN2_000120"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR8929828.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHWN2_000121"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHWN2_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR8929829.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHWN2_000122"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHWN2_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR8929830.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="ACHWN2_000123"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="ACHWN2_000123"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR8929831.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            39902..40003
                     /locus_tag="ACHWN2_000124"
                     /pseudo
     CDS             39902..40003
                     /locus_tag="ACHWN2_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHWN2_000125"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHWN2_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR8929832.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="ACHWN2_000126"
     CDS             complement(41212..42177)
                     /locus_tag="ACHWN2_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR8929833.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHWN2_000127"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHWN2_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR8929834.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHWN2_000128"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHWN2_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR8929835.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="ACHWN2_000129"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="ACHWN2_000129"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR8929836.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHWN2_000130"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHWN2_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR8929837.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHWN2_000131"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHWN2_000131"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR8929838.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHWN2_000132"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHWN2_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR8929839.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHWN2_000133"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHWN2_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR8929840.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHWN2_000134"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHWN2_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR8929841.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHWN2_000135"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHWN2_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR8929842.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHWN2_000136"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHWN2_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR8929843.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHWN2_000137"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHWN2_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR8929844.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="ACHWN2_000138"
     CDS             49715..50680
                     /locus_tag="ACHWN2_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR8929845.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHWN2_000139"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHWN2_000139"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR8929846.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHWN2_000140"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHWN2_000140"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR8929847.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="ACHWN2_000141"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="ACHWN2_000141"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR8929848.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="ACHWN2_000142"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="ACHWN2_000142"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR8929849.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="ACHWN2_000143"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="ACHWN2_000143"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR8929850.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHWN2_000144"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHWN2_000144"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR8929851.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="ACHWN2_000145"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="ACHWN2_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR8929852.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHWN2_000146"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHWN2_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR8929853.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHWN2_000147"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHWN2_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR8929854.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="ACHWN2_000148"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="ACHWN2_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR8929855.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHWN2_000149"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHWN2_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR8929856.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHWN2_000150"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHWN2_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHWN2_000151"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHWN2_000151"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR8929857.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHWN2_000152"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHWN2_000152"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR8929858.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="ACHWN2_000153"
     CDS             complement(62547..63476)
                     /locus_tag="ACHWN2_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR8929859.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="ACHWN2_000154"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="ACHWN2_000154"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR8929860.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="ACHWN2_000155"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="ACHWN2_000155"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR8929861.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..70630
                     /locus_tag="ACHWN2_000156"
                     /pseudo
     CDS             70226..70630
                     /locus_tag="ACHWN2_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
BASE COUNT        17314 a        19175 c        17560 g        16663 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttat
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    70561 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
    70621 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
    70681 tcggtttgta tattcatgca aataaacgaa gg
//
LOCUS       DAZSNO010000003        65867 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZSNO010000003 DAZSNO010000000
VERSION     DAZSNO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65867
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.003"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..452)
                     /gene="folK"
                     /locus_tag="ACHWN2_000157"
     CDS             complement(<1..452)
                     /gene="folK"
                     /locus_tag="ACHWN2_000157"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR8929862.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHT"
     gene            complement(449..1846)
                     /gene="pcnB"
                     /locus_tag="ACHWN2_000158"
     CDS             complement(449..1846)
                     /gene="pcnB"
                     /locus_tag="ACHWN2_000158"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR8929863.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(1906..2802)
                     /gene="gluQRS"
                     /locus_tag="ACHWN2_000159"
     CDS             complement(1906..2802)
                     /gene="gluQRS"
                     /locus_tag="ACHWN2_000159"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR8929864.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(2869..3324)
                     /gene="dksA"
                     /locus_tag="ACHWN2_000160"
     CDS             complement(2869..3324)
                     /gene="dksA"
                     /locus_tag="ACHWN2_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR8929865.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3502..4206)
                     /gene="sfsA"
                     /locus_tag="ACHWN2_000161"
     CDS             complement(3502..4206)
                     /gene="sfsA"
                     /locus_tag="ACHWN2_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR8929866.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4221..4751)
                     /gene="thpR"
                     /locus_tag="ACHWN2_000162"
     CDS             complement(4221..4751)
                     /gene="thpR"
                     /locus_tag="ACHWN2_000162"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR8929867.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4825..7254
                     /gene="hrpB"
                     /locus_tag="ACHWN2_000163"
     CDS             4825..7254
                     /gene="hrpB"
                     /locus_tag="ACHWN2_000163"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR8929868.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7450..9984
                     /gene="mrcB"
                     /locus_tag="ACHWN2_000164"
     CDS             7450..9984
                     /gene="mrcB"
                     /locus_tag="ACHWN2_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR8929869.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10204..12447
                     /gene="fhuA"
                     /locus_tag="ACHWN2_000165"
     CDS             10204..12447
                     /gene="fhuA"
                     /locus_tag="ACHWN2_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR8929870.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTNKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12498..13295
                     /gene="fhuC"
                     /locus_tag="ACHWN2_000166"
     CDS             12498..13295
                     /gene="fhuC"
                     /locus_tag="ACHWN2_000166"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR8929871.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13295..14185
                     /gene="fhuD"
                     /locus_tag="ACHWN2_000167"
     CDS             13295..14185
                     /gene="fhuD"
                     /locus_tag="ACHWN2_000167"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR8929872.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14182..16164
                     /gene="fhuB"
                     /locus_tag="ACHWN2_000168"
     CDS             14182..16164
                     /gene="fhuB"
                     /locus_tag="ACHWN2_000168"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR8929873.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16199..17479)
                     /gene="hemL"
                     /locus_tag="ACHWN2_000169"
     CDS             complement(16199..17479)
                     /gene="hemL"
                     /locus_tag="ACHWN2_000169"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR8929874.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17645..17692
                     /gene="yadX"
                     /locus_tag="ACHWN2_000170"
     CDS             17645..17692
                     /gene="yadX"
                     /locus_tag="ACHWN2_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR8929875.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17704..19125
                     /gene="clcA"
                     /locus_tag="ACHWN2_000171"
     CDS             17704..19125
                     /gene="clcA"
                     /locus_tag="ACHWN2_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR8929876.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19149..19214
                     /gene="yadW"
                     /locus_tag="ACHWN2_000172"
     CDS             19149..19214
                     /gene="yadW"
                     /locus_tag="ACHWN2_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR8929877.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19207..19551
                     /gene="erpA"
                     /locus_tag="ACHWN2_000173"
     CDS             19207..19551
                     /gene="erpA"
                     /locus_tag="ACHWN2_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR8929878.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19598..20221)
                     /locus_tag="ACHWN2_000174"
     CDS             complement(19598..20221)
                     /locus_tag="ACHWN2_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR8929879.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20259..21059)
                     /gene="btuF"
                     /locus_tag="ACHWN2_000175"
     CDS             complement(20259..21059)
                     /gene="btuF"
                     /locus_tag="ACHWN2_000175"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR8929880.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNLVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21052..21750)
                     /gene="mtnN"
                     /locus_tag="ACHWN2_000176"
     CDS             complement(21052..21750)
                     /gene="mtnN"
                     /locus_tag="ACHWN2_000176"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR8929881.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21834..23351
                     /gene="dgt"
                     /locus_tag="ACHWN2_000177"
     CDS             21834..23351
                     /gene="dgt"
                     /locus_tag="ACHWN2_000177"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR8929882.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23481..24905
                     /gene="degP"
                     /locus_tag="ACHWN2_000178"
     CDS             23481..24905
                     /gene="degP"
                     /locus_tag="ACHWN2_000178"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR8929883.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25060..26216
                     /gene="cdaR"
                     /locus_tag="ACHWN2_000179"
                     /pseudo
     CDS             25060..26216
                     /gene="cdaR"
                     /locus_tag="ACHWN2_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26305..26691)
                     /locus_tag="ACHWN2_000180"
     CDS             complement(26305..26691)
                     /locus_tag="ACHWN2_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR8929884.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27005..27829)
                     /gene="dapD"
                     /locus_tag="ACHWN2_000181"
     CDS             complement(27005..27829)
                     /gene="dapD"
                     /locus_tag="ACHWN2_000181"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR8929885.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQATPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27860..30532)
                     /gene="glnD"
                     /locus_tag="ACHWN2_000182"
     CDS             complement(27860..30532)
                     /gene="glnD"
                     /locus_tag="ACHWN2_000182"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR8929886.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30594..31388)
                     /gene="map"
                     /locus_tag="ACHWN2_000183"
     CDS             complement(30594..31388)
                     /gene="map"
                     /locus_tag="ACHWN2_000183"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR8929887.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31756..32481
                     /gene="rpsB"
                     /locus_tag="ACHWN2_000184"
     CDS             31756..32481
                     /gene="rpsB"
                     /locus_tag="ACHWN2_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR8929888.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32739..33590
                     /gene="tsf"
                     /locus_tag="ACHWN2_000185"
     CDS             32739..33590
                     /gene="tsf"
                     /locus_tag="ACHWN2_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR8929889.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33737..34462
                     /gene="pyrH"
                     /locus_tag="ACHWN2_000186"
     CDS             33737..34462
                     /gene="pyrH"
                     /locus_tag="ACHWN2_000186"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR8929890.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34754..35311
                     /gene="frr"
                     /locus_tag="ACHWN2_000187"
     CDS             34754..35311
                     /gene="frr"
                     /locus_tag="ACHWN2_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR8929891.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35403..36599
                     /gene="ispC"
                     /locus_tag="ACHWN2_000188"
     CDS             35403..36599
                     /gene="ispC"
                     /locus_tag="ACHWN2_000188"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR8929892.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36788..37546
                     /gene="ispU"
                     /locus_tag="ACHWN2_000189"
                     /gene_synonym="uppS"
     CDS             36788..37546
                     /gene="ispU"
                     /locus_tag="ACHWN2_000189"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR8929893.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37559..38416
                     /gene="cdsA"
                     /locus_tag="ACHWN2_000190"
     CDS             37559..38416
                     /gene="cdsA"
                     /locus_tag="ACHWN2_000190"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR8929894.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38428..39780
                     /gene="rseP"
                     /locus_tag="ACHWN2_000191"
     CDS             38428..39780
                     /gene="rseP"
                     /locus_tag="ACHWN2_000191"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR8929895.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39810..42242
                     /gene="bamA"
                     /locus_tag="ACHWN2_000192"
     CDS             39810..42242
                     /gene="bamA"
                     /locus_tag="ACHWN2_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR8929896.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42364..42849
                     /gene="skp"
                     /locus_tag="ACHWN2_000193"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42364..42849
                     /gene="skp"
                     /locus_tag="ACHWN2_000193"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR8929897.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42853..43878
                     /gene="lpxD"
                     /locus_tag="ACHWN2_000194"
     CDS             42853..43878
                     /gene="lpxD"
                     /locus_tag="ACHWN2_000194"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR8929898.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43983..44438
                     /gene="fabZ"
                     /locus_tag="ACHWN2_000195"
     CDS             43983..44438
                     /gene="fabZ"
                     /locus_tag="ACHWN2_000195"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR8929899.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44442..45230
                     /gene="lpxA"
                     /locus_tag="ACHWN2_000196"
     CDS             44442..45230
                     /gene="lpxA"
                     /locus_tag="ACHWN2_000196"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR8929900.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45230..46378
                     /gene="lpxB"
                     /locus_tag="ACHWN2_000197"
     CDS             45230..46378
                     /gene="lpxB"
                     /locus_tag="ACHWN2_000197"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR8929901.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46375..46971
                     /gene="rnhB"
                     /locus_tag="ACHWN2_000198"
     CDS             46375..46971
                     /gene="rnhB"
                     /locus_tag="ACHWN2_000198"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR8929902.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47008..50490
                     /gene="dnaE"
                     /locus_tag="ACHWN2_000199"
     CDS             47008..50490
                     /gene="dnaE"
                     /locus_tag="ACHWN2_000199"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR8929903.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50503..51462
                     /gene="accA"
                     /locus_tag="ACHWN2_000200"
     CDS             50503..51462
                     /gene="accA"
                     /locus_tag="ACHWN2_000200"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR8929904.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51561..53702
                     /gene="ldcC"
                     /locus_tag="ACHWN2_000201"
     CDS             51561..53702
                     /gene="ldcC"
                     /locus_tag="ACHWN2_000201"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR8929905.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIAPEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53759..54148
                     /locus_tag="ACHWN2_000202"
     CDS             53759..54148
                     /locus_tag="ACHWN2_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR8929906.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54213..55511
                     /gene="tilS"
                     /locus_tag="ACHWN2_000203"
     CDS             54213..55511
                     /gene="tilS"
                     /locus_tag="ACHWN2_000203"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR8929907.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55560..55820)
                     /gene="rof"
                     /locus_tag="ACHWN2_000204"
     CDS             complement(55560..55820)
                     /gene="rof"
                     /locus_tag="ACHWN2_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR8929908.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55807..56007)
                     /locus_tag="ACHWN2_000205"
     CDS             complement(55807..56007)
                     /locus_tag="ACHWN2_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR8929909.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56173..56718
                     /locus_tag="ACHWN2_000206"
     CDS             56173..56718
                     /locus_tag="ACHWN2_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR8929910.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56715..57137
                     /gene="arfB"
                     /locus_tag="ACHWN2_000207"
     CDS             56715..57137
                     /gene="arfB"
                     /locus_tag="ACHWN2_000207"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFR8929911.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57151..57861
                     /gene="nlpE"
                     /locus_tag="ACHWN2_000208"
                     /gene_synonym="cutF"
     CDS             57151..57861
                     /gene="nlpE"
                     /locus_tag="ACHWN2_000208"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR8929912.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58016..58840)
                     /locus_tag="ACHWN2_000209"
     CDS             complement(58016..58840)
                     /locus_tag="ACHWN2_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR8929913.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58893..60611)
                     /gene="proS"
                     /locus_tag="ACHWN2_000210"
     CDS             complement(58893..60611)
                     /gene="proS"
                     /locus_tag="ACHWN2_000210"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR8929914.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60722..61429)
                     /gene="tsaA"
                     /locus_tag="ACHWN2_000211"
     CDS             complement(60722..61429)
                     /gene="tsaA"
                     /locus_tag="ACHWN2_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR8929915.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61426..61830)
                     /gene="rcsF"
                     /locus_tag="ACHWN2_000212"
     CDS             complement(61426..61830)
                     /gene="rcsF"
                     /locus_tag="ACHWN2_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR8929916.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61948..62763)
                     /gene="metQ"
                     /locus_tag="ACHWN2_000213"
     CDS             complement(61948..62763)
                     /gene="metQ"
                     /locus_tag="ACHWN2_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR8929917.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62803..63456)
                     /locus_tag="ACHWN2_000214"
     CDS             complement(62803..63456)
                     /locus_tag="ACHWN2_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR8929918.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63449..64480)
                     /gene="metN"
                     /locus_tag="ACHWN2_000215"
     CDS             complement(63449..64480)
                     /gene="metN"
                     /locus_tag="ACHWN2_000215"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR8929919.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64668..65243
                     /gene="gmhB"
                     /locus_tag="ACHWN2_000216"
     CDS             64668..65243
                     /gene="gmhB"
                     /locus_tag="ACHWN2_000216"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR8929920.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65607..>65867
                     /locus_tag="ACHWN2_000217"
     rRNA            65607..>65867
                     /locus_tag="ACHWN2_000217"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15573 a        16329 c        18169 g        15796 t
ORIGIN      
        1 cttgtatgta agacttcacg caacgtctcc ccatcaggaa acgccagctc cggtgcaatc
       61 tcaaacagcg gccacagcat aaatccacga ttcttcatat cgtagtgcgg aacggtcagg
      121 cgttcggtat ttatcacttc attaccaaac agcatgatat cgagatccag cgtgcgtggt
      181 ccccagcgtt cagctttacg aacacgaccc tgttgcagtt ctatgcgctg tgtatggttg
      241 agtaactctt ccggggcggg cgaagtttca agcgccacgg cggcgttgag gtaatcgggt
      301 tgatcttgcg gccccagcgg tggggtgcgg taaaacgaag aaacggcaag aatgcggctt
      361 tcagggatat cgcctaatgc tttcagggca gcattgacct gctccagcgg agaggccaga
      421 ttgctgccta tggcaatata cgccactgtc atgtggtacc ctcacgacgc ggtgcgcgtt
      481 tgcgtggacg acgagtacga cgacgcggtg acggttcttc atccagctcg ttaagcatcc
      541 ctttttgatc tggtggtgcg gaaacctgga actcacccca ccatttcacc agacgctgca
      601 gttcagcatt acgttcaact tcagctcgca aggccaacag gtcataagcc gcacggaact
      661 taggatgctc cagcagtttc catgcgcgtt taccctgacg acgggacata cgcaactgca
      721 actgccagat atcgcgggtt aacgtcgtca gacgtttcgg gattgccagt gaacggcagg
      781 cttcgtccag cacgtcgttc atcgccagcg cgaaagcgtc gtgataggtc aggccgcttt
      841 cctgggcgat cttctgtgcc gtctccagca gtgggtacca gaacatggcg gcaaacaaga
      901 acgccgggtt cacgcgcata tcgttatgga tacgcgtatc ggtattcttc agcacctgtt
      961 caatgatccg ctccatcggg ctgtcgccat tttccgtgaa gtagcgggta atggtcggga
     1021 acagcggctg gaacagatga tattcacaca acagcttata ggtttcgtaa ccgtagcccg
     1081 cttgtagcag tttaagcgat tcttcaaaca ggcgtgccgg tgggatatcg ttcagcaggg
     1141 tagcgaggcg agggatcggt tctgcggttt ccgggctgat gcgcataccc aatttggcgg
     1201 caaaacgtac cgcgcgcagc atacgtaccg gatcttcacg gtagcgcgtt tccgggttac
     1261 caatcagacg gataacgccg tccttcagat ccttcatacc accaacgtaa tcacggacgg
     1321 taaaatccgc tacgctgtaa tacaggctgt tgatagtgaa atcgcggcgc tgggcgtctt
     1381 cttcgatgga gccgaaaatg ttgtcgcgca gcaacatgcc gttttgcccg cgttgggagg
     1441 tcgtgcggtc gctgacgtta ccttcgtggt gtccacggaa ggtcgcaact tcgataatct
     1501 ccgggccaaa cattacatga gccagacgga aacggcgacc caccaggcgg cagttacgga
     1561 acagtttgcg cacctgctca ggcgtggcgt tagtggttac gtcaaaatct ttcggctttt
     1621 tgccaagtaa caggtcgcgc acgccgccgc caaccagcca ggcttcgtat cccgctttgt
     1681 tgagcctgta cattaccttc agggcatttt cactgatatc tttgcgggaa atagcatgct
     1741 gctcacgcgg gatcaccgtc acctgtggac gggcgactgc ctgttcagcc tcgctttcct
     1801 cgcggcttag caccttgcgg caaaaattag cgactcgggt aaaaatagta cacctcggta
     1861 gtgtcaaaca tcattcagga caaaaaaata gcggctaatc atagctcagc atgacgcatt
     1921 tgagaatgtt gaatttacaa ttgccgactc gggcacggcg gtcagcatcc agtttttgac
     1981 ggctgactga aggatttgct cgacgctgaa atcctgccag tgtgcttctg cctgctgccc
     2041 cagaaattga agtgccgcga ttagtaccgg gcgtggatcg cctttcggca acgcaggcgc
     2101 atgattctgc ttggaaagtt tagcgccttg tggattaagc gccagcggca gatgaatgta
     2161 atctggcact ttccagccaa aaagctggta cagcgggatt tgccttactg ttggttcaat
     2221 cagatcagcc ccacgcacta tttctgtaac gccctggaaa tgatcatcaa ccacaacagc
     2281 caggttgtag gcgaacaacc catcacggcg atgaatgata aaatcttccc gtgccagttt
     2341 ttcgtcggcg tgaataatgc ctcgcagcag gtcagtaaat tgcgtgaccg gatgctgctg
     2401 gcggatacgc actgcggcgt tgtctggtcc atgatgcaac acccggcaat gaccgtcgta
     2461 aataccgcca atgctttgaa tacgcgcacg cgtacaggtg cagtaataac ttagtccttg
     2521 ttcatgtaac caggcgagtg cttcacgata ggcgtcgtga cgttgcgatt gccagagaac
     2581 atcgccgtcc cagtgcagac cgtaatgttc cagctggcgc aggatagttt ctgcggcacc
     2641 gggaacttca cgaggcgggt cgatatcttc tatgcgtacc agccagcgac cttgccgggc
     2701 gcgagcctgc aaatagctgc cgagcgcggc gatcagagag ccaaaatgaa gctcgccgga
     2761 aggagagggg gcgaagcggc caatatactg tgtgtctgtc atctctttga acaaaaaata
     2821 aggcgggagc atttcccgcc tgtggtaaac gtgatggaac ggctgtaatt agccagccat
     2881 ctgtttttcg cgaatttcag ccagcgtttt gcagtcgatg cacagatcgg ctgtcgggcg
     2941 cgcttccaga cggcgaatac caatttcaac accgcaggat tcgcagtagc cgaaatcttc
     3001 gtcttccact tttttcagcg tcttctcgat ctttttgatc agcttacgct cgcgatcgcg
     3061 gttacgcagt tcgaggctga actcttcttc ctgggctgca cggtctaccg ggtccgggaa
     3121 gttggctgct tcatcctgca tatgtgtaac ggtgcgatcg acttcatccc tgagttgatt
     3181 acgccatgct tccagaatac gacggaagtg cgccagctgg gcttcattca tatactcttc
     3241 gcccggcttc tcctgatatg gttccacccc agcgatggcg agaatactca gggacgatgt
     3301 tttacggttt tgcccttctt gcatgttgct tctccttaac acgcactatc gatccccatg
     3361 ttcgggggaa aaatgaggcc gctataaata gcagatgctt ttccggatag caattatcta
     3421 aacgtaacac ttgacaactg tgtgaggaaa agcgtatttg cgcacgcgac cagaatgtaa
     3481 attaaccagt tacttacttt actacaatgt aaccggcagt gattttttaa gagccatgcc
     3541 ttcagcagaa atttccgctt tgtaagccag aatttctacc cccctctgtt gagcttctga
     3601 caatagttgc gcgtatttct catcgatgtg gcgcgcgggt gaaaaccgtg taatggctga
     3661 atgcagcacg gcgaaaaaaa taaccgcacg ctggccttca gccgctacgc tcatcaactc
     3721 ccgaaggtgt ttctgacctc gttcagtgac cgcatcggga aaatatccct gttcgttctc
     3781 cgctaacgta accgatttca cttcaatata gcagtctgga cgcgaatccg cctgcaacat
     3841 aaagtcaata cggctgcgtt cggagccgta ttttacttcg cttttcagcg agctatagcc
     3901 tgacagttct gaaattgatt cattaaggat agcctctttc gtcaacctgt tagcccaaag
     3961 cgtattgacg caaataaatg cgccgctctg gctttgagtt aattcccagg tgtgtgggta
     4021 tttccgtttg gtgttgtctg aagtcgaata ccagacggta tcgccaggcg ttgcacaacc
     4081 ggtcatcgca cccgtattcg ggcagtgtag cgttaattcg cgaccatcgg gtgtgatcac
     4141 atcggctaaa aaacgtttgt aacgctgaat tagcgtcgcg cgctgtagag ggggagaaaa
     4201 ttccattgcg acaatccttg ttattgcgtt agcgcccagc gttttagcgg cgtgtagcgt
     4261 gtgcgtccac gggcaaacga ggaggcgtaa agggtgaact ccgtcaccgc ataagaccag
     4321 ttaaaacccg gcggcgggaa tgtcaccgcc tcgctggcgt cgcgcaataa ggtaatatgt
     4381 ggatgaaacg gacgattgct ttgaaaacaa ccgctgcggg cagcctgtga acggagcata
     4441 ttcgccagct ggattaagcc gcgtggaggc tgacgcatcc ctaaccacac cacacgcgaa
     4501 cgcagccatt gtccggcgtc atcaagcgtg agtgtgaaac caggttgacg aatccgtccg
     4561 gctaaaagag aaagcgcctt ctctttctct gcgctcactt cgcctaaaaa tgccagagtc
     4621 aggtgcaaat tatcggcggc gaccggacgt cccgcctcag gtgggaagtg tgtggcgcgc
     4681 caatggataa tctgttcgcg gatttctgca ggtaagtcga tagcaaagaa cagacgttgc
     4741 ggttcagaca tgtgaggcac tcggttatga attaccgcga tgctacaatg tggcgcgaag
     4801 aatgttaacc ctctggagcg ttttgtgtcg tcgttgcccg ttgctgccgt cttacctgaa
     4861 ttacttaccg ctctcgattg tgcgccgcag gtattattaa gtgcgccgac cggggccggg
     4921 aaatcaacct ggctgccgct gcaactgctg gcgcatcccg gcattaacgg gaaaattatc
     4981 ctgctggagc cgcgtcgtct ggcggcgcgt aacgtcgcgc aacggctggc ggagctgctt
     5041 aacgaaaagc caggcgatac cgttggctac cggatgcgtg cgcaaaactg cgtcgggctg
     5101 aatacccgcc tggaagtggt taccgaaggc gtgctgacgc ggatgatcca gcgtgacccg
     5161 gaactgagcg gtgttggatt ggtgatcctt gatgagtttc atgagcgcag tttgcaggcg
     5221 gatttggcgt tggcgctgtt actcgatgtg caacaaggtc tgcgtgatga ccttaaactg
     5281 ctgattatgt cggctacgct ggacaatgac cgcttgcagc aaatgctgcc ggaagcgccc
     5341 gtcgtcatct cagaagggcg ctcgtttccg gttgaacgcc gttatttacc gctgcccgcg
     5401 catcagcgtt ttgacgaagc cgttgcggta gccaccgccg aaatggtgcg tcaggaaagt
     5461 ggatcattac tgttattttt acctggcgtc ggagaaattc agcgcgtgca ggaacaactg
     5521 gcttcgcgca tcggcagtga tgtggtgctc tgcccgctgt atggcgtgtt gtcactgaac
     5581 gatcagcgaa aagcgatcct cccggcaccg caagggatgc gcaaagtggt gctggcgacc
     5641 aatattgctg aaaccagttt aaccatcgaa ggtattcgtc tggtggtgga ttgtgcccag
     5701 gagcgtgtgg cgcgctttga tccgcgcacg gggcttacgc ggttgattac tcaacgcgtt
     5761 agtcaggcat ccatgacgca gcgtgccggg cgcgccgggc gtctggagcc gggtatcagc
     5821 ctgcatttaa tcgccaaaga acaagcagaa cgcgccgcgg cgcaaagtga gccggagatc
     5881 ttacaaagcg atctttccgg tttgctgatg gaattactgc aatggggatg cagcgatccg
     5941 gcgcagatga gctggctgga tcaaccgcca acggtgaatc tactggccgc gaaacgcctg
     6001 ttacaaatgc tgggggcgct ggagggtgaa cggcttagtg cgcaagggca aaaaatggca
     6061 gcgctgggta acgatccgcg tttagcggca atgctggtta acgcaaagag cgatgacgaa
     6121 gctgctaccg cggcaaaaat tgccgccatt ctcgaagagc cgccacggat gggcaatagt
     6181 gacctgggcg tggcgttttc gcgcaatcaa ccagcctggc agcaacgtag tcagcaactg
     6241 ttaaaacgct taaacgtacg cggcggtgag gcagacagtt cgcttatcgc gccgctactt
     6301 gccggggcgt ttgccgatcg cattgctcat cgccgtgggc aagatggacg ctatcaactg
     6361 gcaaacagca tgggagcgat gctcgatgcc gacgacgcgc taagccgcca cgaatggttg
     6421 atcgcaccgt tattattgca gggcagcgcc tcgccggatg cgcggatttt actggcgcta
     6481 ccggtcgata ttgatgaatt agtacaacgc tgtccgcagc tggtgcagca atctgacacc
     6541 gtggaatggg atgacgcgca aggtacgctg aaagcctggc gtcggctaca aatcggtcag
     6601 ttgacggtga aagtgcagcc gctggcgaaa ccgtcagaag acgagttgca tcaggcgatg
     6661 cttaatggca tccgtgataa aggtttaagc gtgctcaact ggacggcgga agcggaacag
     6721 ctacgcttgc gtttgttatg cgccgcaaag tggttgccgg aatatgactg gccagcggtt
     6781 gatgatgaaa gtttgttggc gacgctggaa acgtggctgc tgccacatat gaccggcgta
     6841 cattcactac gcggcctgaa atcactcgac atttatcagg cactacgcgg attacttgat
     6901 tggggaatgc agcaacgtct ggatagtgaa ttgcctgcgc attacactgt gccgacggga
     6961 agccggatcg ccattcgtta tcatgaagat aacccgcccg cgctggcggt gagaatgcag
     7021 gagatgtttg gtgaggccac taatccgacg atcgcccagg ggcgcgtgcc gctggtgctg
     7081 aagttgcttt cccctgccca aaggccgctg caaatcacgc gtgatttggg ggccttctgg
     7141 aaaggggcgt atcgtgaggt gcaaaaggag atgaaagggc gttatcccaa acacgtctgg
     7201 ccggacgacc cggcaaacac ggcaccgacg cgacggacga aaaagtattc gtaaggttgg
     7261 ttttctccct ctccctgtgg gagagggccg gggtgagggc atcagcgcgc acgttcaccc
     7321 tcaccctaac cctctccctc aagggagagg ggaccgatcg agcgcaaatt ttgagagata
     7381 tcttcttctg tcttgtaaca gaagaacaga aaatcgggct tttgcgcctg aatattgcgg
     7441 agaaaaagca tggccgggaa tgaccgcgag ccaattggac gcaaagggaa accgacgcgt
     7501 ccggtcaaac aaaaggtaag ccgtcgtcgt tacgaagatg acgatgatta cgacgattat
     7561 gatgactatg aggatgaaga accgatgccg cgcaaaggta agggcaaagg caaagggcgt
     7621 aagcctcgtg gcaaacgcgg ctggctatgg ctactgctaa aactggctat cgtttttgcc
     7681 gtgctgatcg ccatttacgg cgtttatctc gatcaaaaaa ttcgtagccg tattgatggc
     7741 aaggtctggc aactgcccgc ggcagtttat ggccgaatgg tcaaccttga accagatatg
     7801 accatcagca agaacgaaat ggtgaagctg ctggaggcaa cacagtatcg tcaggtgtcg
     7861 aaaatgaccc gtcctggcga atttaccgtg caggccaaca gcattgagat gatccgccgt
     7921 ccgtttgatt tcccggacag taaagaaggg caggtgcgtg cgcgtctgac ctttgatggt
     7981 gatcatctgg cgaccatcgt caatatggag aacaaccgtc agttcggttt cttccgtctt
     8041 gatccgcgtc tgatcaccat gatctcctcg ccaaacggtg agcagcgttt gtttgtgccg
     8101 cgcagtggtt tccctgattt gctggtggat actttgctgg cgacagaaga ccgtcatttt
     8161 tacgagcatg atggaatcag tctctactcc attgggcgtg cggtgctggc aaacctgacc
     8221 gcaggtcgca cggtgcaggg tgcgagtacg ctgacgcaac agctggtgaa aaacctgttc
     8281 ctctccagcg agcgttctta ctggcgtaaa gcgaacgaag cttacatggc gctgatcatg
     8341 gacgcgcgtt acagcaaaga ccgtattctt gagctgtata tgaacgaggt gtatctcggt
     8401 cagagcggcg acaacgaaat ccgcggcttc ccgctggcaa gcttgtatta ctttggtcgc
     8461 ccggtagaag agctaagcct cgaccagcag gcgctgttag tcggtatggt gaaaggggcg
     8521 tccatctaca acccgtggcg taacccgaaa ctggcgctgg agcgacgtaa tctggtgctg
     8581 cgtctgctgc aacagcaaca gattattgat caagaactct atgacatgtt gagtgcccgt
     8641 ccgctggggg ttcagccgcg tggtggggtg atctctcctc agccagcctt tatgcagctg
     8701 gtgcgtcagg agttgcaggc aaaactgggc gataaggtaa aagatctctc cggcgtgaag
     8761 atcttcacta cctttgactc ggtggcccag gacgcggcag aaaaagccgc cgtggaaggc
     8821 attccggcac tgaagaaaca gcgtaagttg agcgatcttg aaactgcgat tgtggtcgtc
     8881 gaccgcttta gtggtgaagt tcgtgcgatg gtcggaggtt ctgagccgca gtttgcgggc
     8941 tacaaccgtg cgatgcaggc gcgtcgttcg attggttccc ttgcaaaacc agcgacttat
     9001 ctgacggctt taagccagcc gaaaatctat cgcctgaaca cgtggattgc cgatgcgcca
     9061 atcgcgctgc gtcagccgaa tggccaggtc tggtcaccgc agaacgatga ccgtcgttat
     9121 agcgaaagcg gcagagtgat gctggtggat gcgttgacac gttcgatgaa cgtgccgacg
     9181 gtaaatctgg ggatggcgct ggggctgcct gcggttacgg agacctggat caaactgggc
     9241 gtaccgaaag atcagttgca tccggttccg gcaatgctgc tgggggcgtt gaacttaacg
     9301 ccaatcgaag tggcgcaggc attccagacc atcgccagcg gtggtaaccg tgcaccgctt
     9361 tctgcactgc gttcggtaat cgcggaagat ggtaaggtgc tgtatcagag cttcccgcag
     9421 gcagaacgcg ctgttccggc gcaggcggcg tatctgacac tatggaccat gcagcaggtg
     9481 gtacaacgtg gtacgggtcg tcagcttggg gcgaaatacc cgaacctgca tctggcaggg
     9541 aaaacaggga ctaccaacaa taacgtagat acctggtttg caggcattga cggcagcacg
     9601 gtgaccatca cctgggtagg ccgtgataac aaccagccga ccaaactgta tggagccagc
     9661 ggggcaatgt cgatttatca gcgttatctg gctaaccaga cgccaacgcc gctgaatctt
     9721 gttccgccag aagatattgc agatatgggc gtggactacg acggcaactt tgtttgcagc
     9781 ggtggcatgc gtgtcttgcc ggtctggacc agcgatccgc aatcgctgtg ccagcagagc
     9841 gagatgcagc agcagccgtc aggcaatccg tttgatcagt cttctcagcc gcagcaacag
     9901 ccgcaacagc aacctgctca gcaagagcag aaagacagcg acggtgtagc cggttggatc
     9961 aaggatatgt ttggtagtaa ttaacatcta agcgtgaaat accggatggc gagttgccat
    10021 ccggtaaaat aacatcccat ctaagatatt aaccctttct tttcatctgg ttgtttatta
    10081 acccttcagg aacgctcaga ttgcgtaccg cttgcgaacc cgccagcgtt tcgaatatta
    10141 tcttatcttt ataataatca ttctcgttta cgttattatt cactttacat cagagatata
    10201 ccaatggcgc gttccaaaac tgctcagcca aaacactcac tgcgtaaaat cgcagttgta
    10261 gtagccacag cggttagcgg catgtctgtt tatgcacagg cagcggttga accgaaagaa
    10321 gacactatca ccgttaccgc tgcacctgcg ccgcaagaaa gcgcatgggg gcctgctgca
    10381 actattgcgg cgcggcagtc agctaccggc actaaaaccg atacgccgat tcaaaaagtg
    10441 ccacagtcta tttctgttgt gaccgccgaa gagatggcgc tgcatcagcc gaagtcggta
    10501 aaagaagcgc ttagctacac gccgggtgtc tctgttggta cgcgtggcgc atccaacacc
    10561 tatgaccacc tgatcattcg cggttttgcg gcagaaggcc aaagccaaaa taactatctg
    10621 aatggcctga agttgcaggg caacttctat aacgatgcgg tcattgaccc gtatatgctg
    10681 gaacgcgctg aaattatgcg tggcccggtt tccgtgcttt acggtaaaag cagtcctggc
    10741 ggtctgttga atatggtcag caagcgtccg accaccgaac cgctgaaaga agttcagttt
    10801 aaagccggta ctgacagcct gttccagact ggttttgact ttagcgatgc gttggatgat
    10861 gacggtgttt actcttatcg cctgaccggt cttgcgcgtt ctgccaatgc ccagcagaaa
    10921 gggtcagaag agcagcgtta tgctattgca ccggcgttca cctggcgtcc ggatgataaa
    10981 accaatttca ccttcctttc ttacttccag aacgagccgg aaaccggtta ttacggctgg
    11041 ttgccgaaag agggaaccgt tgagccgctg ccgaacggta agcgtctgcc gacagacttt
    11101 aatgaagggg cgaagaacaa cacctattct cgtaatgaga agatggtcgg ctacagcttc
    11161 gatcacgaat ttaacgacac ctttactgtg cgtcagaacc tgcgctttgc tgaaaacaaa
    11221 acctcgcaaa acagcgttta tggttacggc gtctgctccg atccggcgaa tgcttacagc
    11281 aaacagtgtg cggcattagc gccagcggat aaaggccatt atctggcacg taaatacgtc
    11341 gttgatgatg agaagctgca aaacttctcc gttgataccc agttgcagag caagtttgcc
    11401 actggcgata tcgaccacac cctgctgacc ggtgtcgact ttatgcgtat gcgtaatgac
    11461 atcaacgcct ggtttggtta cgacgactct gtgccactgt tcaatctgta caatccggtg
    11521 aatcccgatt tcgacttcaa tgccaaagat ccggcaaact ccggccctta ccgcattctg
    11581 aataaacaga aacaaacggg cgtttatgtt caggatcagg cgcagtggga taaagtgctg
    11641 gtcaccctag gcggtcgtta tgactgggca gatcaagaat ctcttaaccg cgttgctggg
    11701 acgaccaata aacgtgatga caaacagttt acctggcgtg gtggtgttaa ctacctgttt
    11761 gataatggtg taacacctta cttcagctat agcgaatcgt ttgaaccttc ttcgcaagtt
    11821 gggaaggatg gtaatatttt cgcaccgtct aaaggtaagc agtatgaagt cggcgtgaaa
    11881 tatgtaccgg aagatcgtcc gattgtagtt actggtgccg tgtataatct cactaaaacc
    11941 aacaacctga tggcggaccc tgagggttcc ttcttctcgg ttgaaggtgg cgagatccgc
    12001 gcacgtggcg tagaaatcga agcgaaagcg gcgctgtcgg cgagtgttaa cgtagtcggt
    12061 tcttatactt acaccgatgc ggaatacacc accgatacta cctataaagg caatacgcct
    12121 gcacaggtgc caaaacacat ggcttcgctg tgggctgact ataccttctt tgacggtccg
    12181 ctttcaggtc tgacgctggg caccggtggt cgttatactg gctccagcta tggtgatccg
    12241 gctaactcct ttaaagtggg aagttatacg gtcgtggatg cgttagtgcg ttatgatctg
    12301 gcgcgagtcg gcatggcggg ctccaacgtg gcgctgcatg ttaacaacct gttcgatcgt
    12361 gaatacgtcg ccagctgctt taacacttat ggctgcttct ggggcgcaga acgtcaggtc
    12421 gttgcaaccg caacctttcg tttctaattt ctcttttggg gcacggattt ccgtgcccat
    12481 ttcacaagtt ggctgttatg caggaataca cgaatcattc cgataccact tttgcactgc
    12541 gtaatatctc ctttcgtgtg cccgggcgca cgcttttgca tccgctgtcg ttaacctttc
    12601 ctgccgggaa agtgaccggt ctgattggtc acaacggttc tggtaaatcc actctgctca
    12661 aaatgcttgg tcgtcatcag cctccgtcag aaggggagat tcttcttgat gcccagccgc
    12721 tggaaagctg gagcagcaaa gcgtttgccc gcaaagtggc ttatttgccg cagcagcttc
    12781 ctccggcaga agggatgacc gtgcgtgaac tggtggcgat tggtcgttac ccgtggcatg
    12841 gcgcgctggg gcgctttggt gcggcagatc gcgaaaaggt cgaggaagct atctcgctgg
    12901 ttggcttaaa accgctggcg catcggctgg tcgatagtct ctctggcggc gaacgtcagc
    12961 gggcgtggat cgccatgctg gtggcgcagg atagccgttg tctgttgctc gacgaaccga
    13021 cttcagcgct ggatatcgcc caccaggttg atgtgctggc gctggtgcac cgtttaagtc
    13081 aggaacgtgg cctgacggtc attgccgtgt tgcacgatat caatatggcg gcacgctact
    13141 gtgattatct ggtcgccctg cgcagtggtg aaatgattgc tcagggaacg cctgcggaaa
    13201 ttatgcgcgg cgaaaccctc gaaatgattt atggcatccc gatgggtatt ttgccgcatc
    13261 cggcgggtgc tgcacctgtg agttttgttt attgatgagc ggcttacctc ttatttcgcg
    13321 ccgtcgactg ttaacggcga tggcgctttc tccgttgtta tggcagatga ataccgccca
    13381 cgcagcggct attgacccca atcgtattgt ggcgctggag tggttgccgg tggaattact
    13441 gctggcgctc ggcatcgtgc cttacggcgt ggcggatacc atcaactatc gcctgtgggt
    13501 cagcgaacca ccattgccgg actcagtgat tgacgtcggt ttgcgtacag aacctaacct
    13561 tgaactgctg accgaaatga aaccgtcgtt tatggtctgg tcggcaggat atggcccttc
    13621 atcagaaatg ctggcacgta ttgcgccggg tcgcggattt aacttcagtg acggcaaaca
    13681 tccgttggcg atggcgcgta aatcgctgac ggaaatggca gatttactta acctgcaaag
    13741 cgcagcggaa acgcatttag cgcaatacga agactttatc cgcagcatga aaccccgctt
    13801 tgtgaagcgt ggtgcacgcc cgttactgct gacgacgctt atcgatccgc gccatatgct
    13861 ggtcttcggt ccaaacagct tgttccagga aattctcgat gagtacggca tcccaaatgc
    13921 ctggcaaggg gaaaccaact tctggggcag taccgccgtc agtatcgatc gtctggcagc
    13981 gtataaagac gttgatgtgc tctgttttga tcacgacaac agcaaagaca tggatgcgct
    14041 aatggcaacc ccgctgtggc aggccatgcc gtttgtccgt acagggcgct ttcagcgcgt
    14101 acccgcagtc tggttttatg gtgcaacgct ttcggcaatg cactttgtgc gtgttctgga
    14161 taacgccatc ggaggtaaag cgtgagtaaa cgaattgcgc ttttcccggc gttattgctg
    14221 gcgctgttag tgattgtcgc tacggcgctc acctggatga acttctcaca ggcgctgccg
    14281 cgcagtcagt gggcgcaggc cgcctggtcg ccggatattg acgtcatcga gcagatgatt
    14341 tttcactaca gcttgttgcc gcgtctggcg atttcgctgc tggtgggcgc gggcctgggg
    14401 ctggtgggcg tgctgtttca gcaagtactg cgtaacccgc tggcggaacc gacgacgttg
    14461 ggcgttgcaa caggcgcgca actggggatt accgtcacca cgctctgggc gatccctagc
    14521 gcgatggcga gccagtttgc tgcgctggca ggggcttgcg ttgttggctt aattgtcttt
    14581 ggcgtcgcgt gggggaaacg gctgtcgccg gtaacgctga ttttcgcggg gttggtagtg
    14641 agcctttatt gcggcgcaat caatcagtta ctggttatct tccatcatga ccagttgcaa
    14701 agcatgtttc tgtggagcac tggaacgctg acgcaaaccg actggggcgg tgttgagcgt
    14761 ttatggccgc agttgctggg cggtgtgatg ctgacgttgc tgctacttcg tccgttaacc
    14821 ctgatggggc ttgatgatgg cgtggcgcgc aatctcgggc tggccttgtc gcttgcgcgt
    14881 ctggcggcgc tgtcgctggc gattgtcatc agtgcgctgc tggtgaacgc tgtggggatt
    14941 atcggcttta tcgggttgtt cgcgccgctg ctggcaaaaa tgctgggggc gcggcgtctg
    15001 ctgccacgac tgatgctggc gtcgttgatt ggtgcgctga tcctctggct ttccgatcaa
    15061 atcatcctct ggctgactcg cgtgtggatg gaagtgtcca ccggttcggt cactgcgttg
    15121 atcggtgcgc cgctgctact gtggctgctg ccgcgtttac gcagcattag cgcgccggat
    15181 atgaaggtta acgatcgtgt cgcgactgaa cgccaacatg tgctggcgtt tgccctcgcg
    15241 ggcggcgtgc tgttgttgat ggctgtggtg gtggcgctgt cgtttggtcg tgatgcgcac
    15301 ggctggacgt gggcgagcgg ggcgttgctc gaggatttaa tgccctggcg ctggccgcga
    15361 attatggcgg cgctgtttgc gggcgtcatg ctggcggtag cgggctgtat tattcagcga
    15421 ctgaccggca acccgatggc aagcccggaa gtgctgggga ttagctccgg cgcggcgttt
    15481 ggtgtggtgt taatgctgtt tctggtaccg ggtaatgcct ttggctggtt gttacccgca
    15541 ggcagtctcg gggcggcggt gacgctgttg atcattatga tcgccgccgg acgcggtgga
    15601 ttttccccac accgcatgtt actggcgggg atggcgttaa gtaccgcgtt caccatgctt
    15661 ttgatgatgt tgcaggcaag tggtgacccg cgaatggcgc aagtgctgac ctggatttcc
    15721 ggttcgacct acaacgcgac cgatgctcag gtctggcgca ccggaattgt gatggtgatt
    15781 ttgctggcga ttactccgct gtgccgccgc tggctgacca ttttaccgct gggtggtgat
    15841 accgcgcggg cggtagggat ggcgctgacg ccgacgcgaa ttgcgctgct gctgttagcg
    15901 gcttgcctga cggcgaccgc gacgatgact attggaccgt tgagttttgt tggtttaatg
    15961 gcaccgcata ttgcgcggat gatgggcttt cgacggacga tgccacacat cgtaatttcg
    16021 gcgctggtgg gtggtttact gctggtgttc gctgactggt gtgggcggat ggtgctgttt
    16081 ccattccaga tcccggcggg gctgctgtcg acctttatcg gtgcgccata ttttatctat
    16141 ttgctgagaa agcagagccg ttaatagaat gcctcgcccg gtgggcgagg caaaattatc
    16201 acaacttcgc gaacacccga cgtgcagcat cgatggtgtt attgatatct tccatgctgt
    16261 gcgccacaga cataaagcct gcttcaaacg ctgacggtgc caggtaaaca ccttcatcca
    16321 gcatcatatg gaagaaacgc ttaaaacgct cgacgtcaca ggccatcaca tcctgatagc
    16381 acgtcacgga ctcggcgtcg gtaaagaaga taccgaacat accaccgacg tggttaacga
    16441 ccagcggaat tcccgcttct tctgccgctt ccagcagacc ttctgccaga cgtgttgtca
    16501 gctcatccag cgtttcgtga atgcccggct gcgcgacttc attcagacag gcgaaacccg
    16561 ctgccatcgc aatcgggtta ccggaaagcg tacccgcctg atagaccgga cctgtcgggg
    16621 ccagcgcatc cattacatca cgacgaccac cgaatgcgcc taccggcatt ccaccgccga
    16681 tgatttttcc gaggcaggtt aaatctggca ctacggcgaa ataatcctgt gcgccagcta
    16741 gcgctacgcg gaaaccggtc atcacttcat cgatgatcag caacgcgcca aattcgtcgc
    16801 acagcgcacg cagacctggc aggaactctg gcagcggcgg aacacagttc atattgcctg
    16861 ccaccggctc gacgataata caggcaatct cttgcgggta ttgctcaaac gcggcgcgta
    16921 cagaggccag atcgttataa gtacaggtta aggtatgttt ggcgaaatct gccggaacgc
    16981 ccggcgagtt tggctggcct aacgtgagtg cgccagaacc ggctttcacc agcaggcagt
    17041 cagcgtgacc gtggtaacaa ccttcaaatt taatgatttt gtcgcgaccg gtaaaaccac
    17101 gggccaggcg gatggcgctc atcgtcgcct cggtgccgga gttcaccatg cgcaccatat
    17161 ccatggtcgg gaccagttcg gtcaccagtt gcgccatttt cacttccatt tcggtcggtg
    17221 caccaaagct taaaccacgc tcggcggctt caatcacggc attgcggatc gccggatggt
    17281 tatggcccag caccatcggc ccccaggaac cgacataatc gatataggct ttgccatcaa
    17341 catcgtacag ataagcgccg tccgcttttt cgataaacag tggagtgccg cccacgccag
    17401 taaaggcgcg aacaggggag ttcacaccgc cagggatcag ctcgcgcgct gcgctgtaaa
    17461 gattttcaga cttactcatg gagggttcct gattcgtaga aaaagtgaat ggctgctatt
    17521 ccatgttatt cataacaagt taaatactcg tcaaacatca ggctgcttgt actggtgcaa
    17581 tcctgatttc ttagagtata aaagttttgt gcatttgaaa cattacgctt tgcaaaggat
    17641 tttcatggaa cgtgcgagta aaatgccgtc atcttatttg tatgaccaat aagtgatcat
    17701 tggatgaaaa ctgatactcc ctctttagaa acaccgcagg ccgcgcgcct gcgacgcaga
    17761 caactgattc gccaacttct tgagcgcgat aaaaccccgt tagccatttt gtttatggcg
    17821 gcagtcgtcg gcacgcttgt cgggctggct gcggttgctt ttgacaaagg tgtcgcctgg
    17881 ttgcagaacc aacgtatggg ggcgctggta catactgctg ataattatcc gctactgtta
    17941 accgtcgctt ttctctgttc ggcggtgctg gcgatgttcg gctacttttt ggtgcgcaaa
    18001 tacgcgcccg aagcaggggg atcggggatc ccggaaattg aaggggcgct ggaagatcaa
    18061 cgtcctgttc gctggtggcg tgtattgccg gtgaaattct ttggcgggct ggggacactc
    18121 ggcggaggca tggggctagg gcgcgaagga ccaaccgtgc agatcggcgg caatatcagt
    18181 cgcatggtgc ttgatatttt ccgcctgaaa ggtgatgaag ctcgccatac gctgctggca
    18241 accggtgctg ctgcggggct ggctgcggcc tttaacgcgc cgctggcggg tattttgttt
    18301 attatcgaag agatgcgtcc gcagtttcgc tatacgttaa tttcgattaa agcggtattt
    18361 attggcgtca ttatgtcgac cattatgtac cggattttta atcatgaagt tgcgttgatt
    18421 gacgtcggta aactttctga tgcgccgctt aatacgctgt ggctttatct gatcctcggt
    18481 attatttttg gcattttcgg ccctattttt aataaatggg tgctggggat gcaggatttg
    18541 ctgcaccgtg tgcacggcgg caatattacc aaatgggtac tgatgggggg ggcgattggc
    18601 ggtctgtgtg gattgctggg gtttgtggca ccagcaacgt cgggcggcgg ttttaacctg
    18661 attcctatcg ctaccgcggg gaatttcagc atgggaatgc tggtgtttat cttcgtcgct
    18721 cgggtcatta ccaccttact ctgcttctct tccggcgcgc cgggcggtat ttttgccccg
    18781 atgctggcgc tgggtactgt gctgggaacc gctttcggaa tggttgccgt tgagctgttt
    18841 ccgcaatatc acctggaggc ggggacgttt gctattgccg gaatgggggc attactggcg
    18901 gcatctattc gcgcgccgtt aacggggatc attctggttc tggagatgac cgataactac
    18961 cagctcattt tgccaatgat tattaccggt cttggcgcaa cactattagc gcaatttacc
    19021 ggcgggaaac ctctatactc ggcgattctt gcgcgcacgc tggcaaaaca ggaagctgag
    19081 caactggcgc gaagcaaggc cgcatcagcc agcgagaata cttgaacgaa ataccagggt
    19141 attagataat ggcgattatt attgggttag aatttgcccg attgccgatg tcgtttggag
    19201 caaaatatga gtgatgacgt agcactgccg ctggagttta ccgacgcagc agccaacaaa
    19261 gttaaaagcc tgatcgctga cgaagataac ccgaatctga aattacgcgt gtatatcacc
    19321 ggtggcggtt gcagcggctt ccagtatggt ttcacctttg atgatcaggt gaacgaaggc
    19381 gatatgacca tcgaaaaaca gggcgttggc ctggtggttg atccgatgag cctgcaatat
    19441 ctggtcggcg gttccgttga ctacaccgaa ggactggaag gttctcgttt catcgtgacc
    19501 aacccgaacg cgaaaagcac ctgcggttgc ggttcttcct ttagcatcta atctgttgtt
    19561 catgtgccgg ataaactatc cggcacatct tctctcttca acgcccattc tcatccagcg
    19621 caaacgtcgg tagcttaaga tgccaacgaa tagccgccag ccgaatcaat agcgtcacga
    19681 ccatgcccat catactggct gtttccagtg gtacggaaaa tgtgtaataa gccgtagcgt
    19741 ggacaatacc gccgataata caggcagttg cgtagatttc tgtacgtaaa atcatgggga
    19801 tttcgcggac cagtacatca cgaatgatcc cgccgccaac gccagtaatg acgcccatac
    19861 aaaccgcgat taacggaccg gcttccgcat taaaggcttt attcacgcta atgccgacaa
    19921 acaccgccag accaacggcg tccagcaccg gcaacatcca ttttggtaag cgtcttggct
    19981 ggcgcaccag cacgatggtc agcatgctgg tgaccatggc aacgaccaga tcggtgggat
    20041 ctttcaccca aaataccggg ccgtgatcca gcgccatgtc gcgaattgtt ccgtcgccta
    20101 ctgcggtaac cacgcccagt accagaacac caaaagggtc catacgcaat tttccggcta
    20161 acaaaacgcc gtagatggca aataccgctg tgccgactat atccagccaa tagacgagca
    20221 ttgttcaatc cccactgagc acctgaaaag gtcaggcact aatctacctg tgaaagcgca
    20281 ttacagagct gttgtgctgc gaggataata cgtgggcttg cacgttcaaa ccagtcactc
    20341 gtgagaggaa taacgggaat tttgagctgt tcaccccagt attgtttgat tttaggaatt
    20401 tggtccggtc cgcctgtaat gacaatagcc tgtggcgagc gtgctaacac ctgttcgcgg
    20461 ctaacttgcg gccagggaac ccggctgtct ttaaagatgt tttctccgcc acaaacttcg
    20521 agtaccaggt tctgaatcga ctcttttcca ctggtaaatg gcggattaat gccgaattgc
    20581 agaaaaacac gttttttagg tttatcagca tattgcgctt tcaattgcac gtactgatcc
    20641 agcagggatt gcgcggcttg ttcggccttg tctggttgcg gactccaggg ggccagttga
    20701 cgtaacgcat tggcaatttg ttcaatgctt gtcgcatcga cccacatcac ttttattccc
    20761 agcgaagcca gctggtcaac ctgccgctcg gcattacctc cacgccaggc aatcaccaga
    20821 tcgggtttca gcgcgacaat gcgttccaga ttcatcccct gccaggtgga aacctgctca
    20881 atcttttgcg cttgtggagg atagtcggaa tagctgctga ccccaaccgg cgtgatcccg
    20941 gcggcaaagg caagttcagt gttggcggga gaaagcgtga tgacgcgcgg cgcggcgttg
    21001 agccacagtg gcgcgagaaa agacagggcg accagcgccc tgaacagtga tttagccatg
    21061 tgccagtttc tgcaccagtg actcaaccat caggctggac tgtttagcgg caacagccag
    21121 gaactcatcg aagctaagat gagactgttg atcggccacg tcggagatgg cgcgtacgac
    21181 aacaaacggg acgttgaaat tgtggcagac atgggcgatt gccgtcgctt ccatctctac
    21241 agcaatggcc tgtgggaagt tgtggcggat tttcgccaga ccaacagaac cgttgatgaa
    21301 agcgtcgccg ctaacaatca ggccacgtac agcgttaaga ttcagttcgg caatgcaggc
    21361 ctcagcggca gcgatcagtt tatcgtcagc tttaaagcct gccggacagc ctggtaactg
    21421 accgtattca taaccaaatg ccgtgacatc cgcgtcgtga taacgcgctt cgtccgagac
    21481 aacgatatcg cccactttca gcgttggtgc caggccaccg gcagaaccgg tgttaataat
    21541 cacatctggc ttgcagtgtt ccaacagcag agtggcaccc agcgccgcag cgactttacc
    21601 gatgcccgat ttcagaagcg caacctcggt tccattcagt tggccggtat agatttcgca
    21661 accgccgaga ctgatagttt gacggttttc gattttgtca cgcagcagcg taacttcttc
    21721 ttccattgca ccaatgatgc cgattttcat agatttactc gcgataagcc cgatttgaag
    21781 gcatagttta ccatgcgctt acggggaagc gtatttctca cgcgggagag gacatggcac
    21841 agattgattt ccgaaaaaaa ataaactggc atcgtcgtta ccgttcaccg cagggcgtta
    21901 aaaccgaaca tgagatcctg cggatcttcg agagcgatcg tgggcgtatc atcaactctc
    21961 cggcaattcg tcgtctgcaa caaaagaccc aggtttttcc actggagcgc aatgccgccg
    22021 tgcgcacgcg tcttacccac tcgatggaag tccagcaggt gggacgctac atcgccaaag
    22081 aaattttaag ccgtctgaaa gagcttaaat tgctggaagc atacggcctg gatgaactga
    22141 ccggcccctt tgaaagcatt gttgagatgt catgcctgat gcacgatatc ggcaatccgc
    22201 cgtttggtca ttttggcgaa gcagcgataa atgactggtt tcgccaacgt ttgcacccgg
    22261 aagatgccga aagccagcct ctgactgacg atcgctgcag cgtggcggca ctacgtttac
    22321 gggacgggga agaaccgctt aacgagctgc ggcgcaagat tcgtcaggac ttatgtcatt
    22381 ttgaggggaa tgcacaaggc attcgtctgg tgcatacatt gatgcggatg aatctcactt
    22441 gggcacaggt tggcggtatt ttaaaatata cccgtccggc gtggtggcgt ggcgaaacgc
    22501 ctgagacaca tcactattta atgaaaaagc cgggttatta tctttctgaa gaagcctata
    22561 ttgcccgctt gcgtaaagaa cttaatttgg cgctttacag tcgttttcca ttaacgtgga
    22621 ttatggaagc tgccgacgac atctcctatt gtgtggcaga ccttgaagat gcggtagaga
    22681 aaagaatatt taccgttgag cagctttatc atcatttgca cgaagcgtgg ggccagcatg
    22741 agaaaggttc gctcttttcg ctggtggttg aaaatgcctg ggaaaaatca cgctcaaata
    22801 gtttaagccg cagtacggaa gatcagtttt ttatgtattt acgggtaaac accctaaata
    22861 aactggtacc ctacgcggca caacgattta ttgataatct gcctgcgatt ttcgccggaa
    22921 cgtttaatca tgcattattg gaagatgcca gcgaatgcag cgatcttctt aagctatata
    22981 aaaatgtcgc tgtaaaacat gtgtttagcc atccggatgt cgagcagctt gaattgcagg
    23041 gctatcgggt cattagcgga ttattagaga tttatcgtcc tttattaagc ctgtcgttat
    23101 cagactttac tgaactggta gaaaaagaac gggtgaaacg tttccctatt gagtcgcgct
    23161 tattccacaa actctcgacg cgccatcggc tggcctatgt cgaggctgtc agtaaattac
    23221 cgtcagattc tcctgagttt ccgctatggg aatattatta ccgttgccgc ctgctgcagg
    23281 attatatcag cggtatgacc gacctctatg cgtgggatga ataccgacgt ctgatggccg
    23341 tagaacaata accaggcttt tgtaaagacg aacaataaat ttttaccttt tgcagaaact
    23401 ttagttcgga acttcgggct ataaaacgaa tctgaagaac acagcaattt tgcgttatct
    23461 gttaatcgag actgaaatac atgaaaaaaa acacattagc actgagtgca ctggctctga
    23521 gtttaggttt ggcgttatct ccgctctctg caacggcggc tgagacttct tcagcaacga
    23581 cagcccagca gatgccaagc cttgcaccga tgctcgaaaa ggtgatgcct tcagtggtca
    23641 gcattaacgt agaaggtagc acaaccgtta atacgccgcg tatgccgcgt aatttccagc
    23701 agttcttcgg tgatgattct ccgttctgcc aggaaggttc tccgttccag agctctccgt
    23761 tctgccaggg gggccagggc ggtaatggcg gcggccagca acagaaattc atggcgctgg
    23821 gttccggcgt catcattgat gccgataaag gctatgtcgt caccaacaac cacgttgttg
    23881 ataacgcgac ggtgattaaa gtccagctga gcgatggtcg taagttcgac gcgaagatgg
    23941 ttggcaaaga tccgcgctct gatatcgcgc tgatccaaat ccagaacccg aaaaaactga
    24001 ccgcaattaa gatggcggat tctgatgcac tgcgcgtggg tgattacacc gtagcgattg
    24061 gtaacccgtt tggtctgggc gagacggtaa cttccgggat tgtctctgcg ctggggcgta
    24121 gcggcctgaa tgctgaaaac tacgaaaact tcatccagac cgatgcagcg atcaaccgtg
    24181 gtaactccgg tggtgcgctg gttaacctga acggcgaact gatcggtatc aacaccgcga
    24241 tcctcgcacc ggacggcggc aacatcggta tcggttttgc tatcccgagt aacatggtga
    24301 aaaacctgac ctcgcagatg gtggaatacg gccaggtgaa acgcggtgag ctgggtatta
    24361 tggggactga gctgaattcc gaactggcga aagcgatgaa agttgacgcc cagcgcggtg
    24421 ctttcgtaag ccaggttctg cctaattcct ccgctgcaaa agcgggcatt aaagcgggtg
    24481 atgtgatcac ctcactgaac ggtaagccga tcagcagctt tgccgcactg cgtgctcagg
    24541 tgggtactat gccggtgggc agcaaactga ccctgggctt actgcgcgac ggtaagcagg
    24601 ttaacgtgaa cctggaactg cagcagagca gccagaatca ggttgattcc agctccatct
    24661 tcaacggcat tgaaggcgct gagatgagca acaaaggcaa agatcagggc gtggtagtga
    24721 acaacgtgaa gacgggcact ccggctgcgc agatcggcct gaagaaaggt gatgtgatta
    24781 ttggtgcgaa ccagcaggca gtgaaaaaca tcgctgaact gcgtaaagtt ctcgacagca
    24841 aaccgtctgt gctggcactg aacattcagc gcggcgacag caccatctac ctgttaatgc
    24901 agtaatctcc ctcaacccct tcctgaatac gggaaggggt tctccttaca atctgtgaac
    24961 ttcgccacaa ctccatacat cttcatcatc ctttgggcat ttgcacaatg ccgtacgtta
    25021 cgtacttcct tatgctaagc cgtgcataac ggaggactta tggctggctg gcatcttgat
    25081 accaaaatgg cgcaggatat cgtggcacgt accatgcgca tcatcgatac caatatcaac
    25141 gtaatggatg cccgcgggcg aattatcggc agcggcgatc gtgagcgtat tggtgaattg
    25201 cacgaaggtg cattgctggt actttcacag ggacgagtcg tcgatatcga tgacgcggta
    25261 gcacgtcatc tgcacggtgt gcggcagggg attaatctac cgttacggct ggaaggtgaa
    25321 attgtcggcg taattggcct gacaggtgaa ccagagaatc tgcgtaaata tggcgaactg
    25381 gtctgcatga cggctgaaat gatgctggaa cagtcgcggt tgatgcactt gttggcgcag
    25441 gatagccgtt tgcgggaaga actggtgatg aacctgattc aggcagagga gaatactccc
    25501 gcacttctga atgggcgcaa cggctgggga tcgatctcaa tcaaccgcga gtggtggcta
    25561 ttgttgaggt cgacagcggt cagcttggcg tggacagcgc aatggcggag ttacaacaac
    25621 tgcaaaacgc gctgactacg cccgagcgta ataatctggt ggcgattgtc tcgctaaccg
    25681 aaatggtggt gttgaaaccg gcgttgaact cttttgggcg ctgggatgca gaagatcatc
    25741 gtaagcgagt tgaacaactg attacccgca tgaaagagta cggccagctg cgttttcgcg
    25801 tttcactggg caactatttt accggtcctg gcagtattgc ccgatcctat cgtacggcga
    25861 aaacgacgat ggtggtgggt aaacagcgga tgccagaaag tcgctgctat ttttatcagg
    25921 atctgatgtt acctgtgtta ctcgacagtt tgcgtggcga ctggcaggcc aacgaactgg
    25981 cgcgaccgct ggcgcggctg aaagcgatgg acaataacgg cttgctgcga cgaacgctgg
    26041 cggcgtggtt tcgccacaat gtgcaaccgc tggcaacgtc aaaggcgttg tttattcatc
    26101 gtaataccct ggagtatcgg cttaatcgta tatcggaact gaccgggctt gatttgggca
    26161 attttgatga caggttgctg ctgtatgtgg cgttacaact ggatgaagag cggtagatta
    26221 tgcgttaagg ttgaggcggc gctggctcat tcccctcagc ccgaagggga gaggggaatg
    26281 cccgagttaa ccgtcaattc gggattattt acgcgttagt ttttccagat cggcttcaat
    26341 ctcgctgatc ttattggtta cgactgactc caggtgacgt aagtcgtcga ggatcttacg
    26401 cttaagatca acttcgctgc ggtcacgctg acagatttga tcaagctcat caatgatata
    26461 ccgtagattc gggctgattt cctggacttc tttataaccc tgacccacac catcagcgac
    26521 gaccgtttta cgctgacgcg gatatttaaa cttaacgctc ttggcgaaaa actcgccttt
    26581 gtctttctgg aaatagattt tcagaatatc gttgttggct tcctgccgga ggctgtaacg
    26641 atcaatttct tcaggattgg taatacccag acttttcaga ttgtcgtaca tagcgttacc
    26701 tcaaaatgag tcagtaaatt gtgcttattt tagcatttgg cttggcccgc ccggctcgac
    26761 tgtgatcgca gaaagccttt ttcttccaga cttctatcat ggcgtaaaaa tcaaaaaatt
    26821 acctgcttta ttgtggcgat attccatggg atggattagc acatttatct tcgttttgcg
    26881 aattacatga cagtccgaaa caagatgaaa aagtatttaa aaaaggggga ggaagaatga
    26941 gtaggacgaa ttattctgag ccagaaagca tacgggcagc atcatgctgc ccgtatatgt
    27001 actcttaatc gatggtacgc agcagttcgt taatgccgac tttgccgcga gttttcgcgt
    27061 caactttctt aacgataacc gcacagtaga ggctgtattt gccatctttt gacggcagat
    27121 tacctgaaac aaccacagac cccgccggaa cgcgaccgta gtggatttcg ccggtttcac
    27181 ggtcgtaaat acgggtgctc tgaccaatgt atacgcccat agagatgacg gaaccttctt
    27241 cgacaatcac cccttcaacc acttcagagc gtgcgccaat gaagcagtta tcttcaataa
    27301 tggtcggggt agcctgcagc ggctccagca cgccgccgat gcccacgcca ccggaaaggt
    27361 ggacgttttt accaatctgc gcacaagaac cgacggtcgc ccaggtatca accatggtgc
    27421 cttcatcaac atatgcgccg atgttaacgt aagacggcat cagcacggtg ttacgggcaa
    27481 taaacgcacc ctgacgtacc gccgctggtg gcacaacgcg gaagccttct ttctggaaac
    27541 gtgcttcgtc gtagtcggcg aatttcatcg gcactttgtc gaagtagcgg ctttctgccc
    27601 cttcgatcac ctgattatca ttaatacgga aagagagcag caccgctttt ttcaaccact
    27661 gatgcgtcac ccactgaccg tcaatttttt ccgctacacg cagtgcgccg gaatccagca
    27721 gggcgatcac ctgatttacc gcttcgcggg taacggtgtc tgcattggct ggcgtgatct
    27781 cggcacggcg ttcaaaagcg gtttcaataa tgttctgtaa ctgctgcatt gttaaactct
    27841 tttcatatca gtaaacacat caccctttat cgtttggatt gagggcctct gtcaaccgct
    27901 gatgcacttc ctgctgcaac tcgttattaa gcgcacgccg gtcagcggtg gcaattatga
    27961 ataaatcttc tactcgctcg ccaatggttg taattcgggc accatgaagc gaaattccca
    28021 gatcggcaaa aattttcccg actcgcgcca gcagtccagg ttggtcgagg gcgatcagtt
    28081 cgaggaacga tttgcggtcg gtatgggtcg gcaaaaacgt tacttcggtt tcaacagtaa
    28141 aatggcgtaa tttggcgggt tggcgacggg gctgcggtgg ctgccagcta ctttgcgtca
    28201 gcacctgctc cagaccaaac cgaataacct catggcgatc tgcggacagc gggctgccat
    28261 cgggttccag cacgataaag gtatccatcg ccataccgtc gcgagtggtg aaaatttgtg
    28321 cgtcgtgaac acttaaattg cggcggtcta attcggcaca gacggcggca aacagatagg
    28381 ggcggtccgg gctccagata aaaatctcgg tgcctccacg cgtagcctgc gggctaagca
    28441 ataccagcgg tttgcttaaa tcatgctgta ataaatggcg ggcatgccag gccagttgat
    28501 ttgggctatg gcggacaaaa tagttagcac gacagcgtga ccaaatttgg tgcagcgcct
    28561 cttcgtcgat gttatccatg cgcagtagtg ccagtgcctg gagttggtga tggcgaaccc
    28621 gttcgcgcat atccggcgtg ttttgcattc cgcgtcgtag ctgcttttcg gtggcaaaat
    28681 agagttcgcg caacagactt tgcttccagc tattccacag tgtttcgttg gtggcgcaaa
    28741 tgtcagccac tgtcaggcat accagatagc gcagacgatt ttccgtttga acttcttcgg
    28801 caaactgctt gatgacttca gggtcctgaa tatcgcggcg ttgtgctgtc accgacatca
    28861 gcaagtgctg gcgaaccagc caggcaacca gctgtgtttc gcgtgagttc agcccgtgga
    28921 gttctgcaaa atgcactaca tcctgagcac cgagaatgga gtggtcgccg ccgcgtcctt
    28981 tggcgatatc gtgaaacagc gcggcgatga aaatcagctc agttgacggc aggcgcggcc
    29041 agacgtccac acacaacgga tggcgctggc gcgtttcttc actggcaaaa ctctccagtt
    29101 tcagcatcac gcggatagta tgttcatcca ccgtgtaggc gtggaacaga tcaaactgca
    29161 tctgcccgac gatatgcgac cattgcggca tatacgcgcc gagcacgcta tggcgatgca
    29221 ttggcaatag tccgcgccgc accgctccgg ggtgacgcag aatgctcaaa aacagttttc
    29281 gtgcttgcgg aatattacac agcggttgtt gcagatggcg acgggcatgg cgtaactggc
    29341 gcagcgtggt ggagtaaatg ccggtgatcg cactgttgcg caccatggtg tagaacatac
    29401 gcaagatggc ttccggctgg cgcataaata gtgtttcatc acgcaggtcg attagcgtac
    29461 cgcgtagctg aaactcatcg tcgagtggac gtggtttttc gtcggcggga agggcgagga
    29521 tggcttcatc gaacagttgc agcagcatct ggttgagttc actgacgcgg cgtgtaacgc
    29581 ggaagtaatc cttcatcatc cgctcgaccg gttcgttgcc ttcaccactg taattcagac
    29641 gctgagcgac gctaagttgg cgatcgaata acagacgatt atcgtaacgg ctgacgacca
    29701 gatgcagggc aaagcgaata cgccacagta tatgcagaca ttcgtttaat tccgcccgct
    29761 ccgctgaggt taagaagcca aacccgacca tttcatccag cgatgttgcg ccaaaatgac
    29821 ggcgggccac ccattgcaga gtgtgaatat cgcgcaagcc gccagggcta cttttgatgt
    29881 ctggttcaag gttgtagctg gtgccatggt aacgctgatg gcgctggttc tgttcttcaa
    29941 ctttcgctgc gtagaacttg tcggaaggcc agaatccttc gctaaaaata tgtttttgca
    30001 gttcgaggaa gagcgcaaca tcgccaatta ataagcggga ttcgattaaa ttggtggcga
    30061 cggttaaatc cgataaccct tccaacatgc actcttcaag cgtgcgcaca ctatgaccga
    30121 cttccagctt tacatcccag agcagcgtta acagctcgcc cactttttgc gcctgatcgt
    30181 ccgggagctt tttacggctt aaaatcagta aatcgatgtc agaaagtgga tgcagctcgc
    30241 cacgaccgta gccaccgacg gcaaccaatg ccaggtcggc aatctggctg aatcccgctt
    30301 caatccataa tcgttgcagg agctggtcta tgaactcggt acgtgcctca atcaactgtt
    30361 ctgcagagat cccattgtca aaggcatcac ccagccaacg ctggaaagta tcgatatggg
    30421 cttttatccc acagacggtt aattcatcac ggggccaggc gcatggattt tgcggttgac
    30481 cgggcagggt ggagagagcg gtgtttgcgt actgttctgg aagggtattc attgtgcgcc
    30541 acccataaga ttaaattatc gcattaaaaa agccggcatt cgccggcttc atcttattcg
    30601 tcgtgcgaga ttatcgccgg gatggtgtca tccttgcgta gcgtcaaaat ttcgcagccg
    30661 ttatcagtca ccacaatagt atgctcatat tgtgcagaca agctgcgatc tttggttttt
    30721 accgtccagc catctttcat ggtgcggatc tcttttttac ccgcgttgac cattggctca
    30781 atggtgaacg tcatccccgg tttcagcacg acgttggttt cacgggagtc atagtgcagc
    30841 acctgcggtt cttcatggaa gccgcgacca ataccgtgtc cgcaatattc acgaacgacg
    30901 gagaagcctt ctgcttcgac aaatttctga atcgccgcac cgatttcgcg cagattaatg
    30961 cctggtttta ccatgcgtag cgccaggtac aggctttctt gcgtgatgcg gcacaggcgt
    31021 tcgcccatga tggtcggctt accgacgata aacattttcg aggtatcgcc gtggaaacca
    31081 tctttgatta cggtgacatc aatgttaacg atatcgccat ctttcagcag cttagcatcg
    31141 tccgggatac cgtggcacac cacttcatta atagagatgc aaacggattt cggatagccg
    31201 tgatagccga ggcaggcaga aaccgcgtgt tgttcattaa caatgtaatc attacagatg
    31261 cgatccagct cgccggtgct gacgcccggt ttaacatacg gttcgatcat ctccagcact
    31321 tcggcagcca gtcggccagc gacgcgcatt ttttcgatat cttctggggt cttgattgag
    31381 atagccatta attctgtcca tcagcgtcgg tgataccgac aatatatatg taagtgccgt
    31441 caatggtatc acacccgggc aaattgagaa tcattctgaa tttcgccaaa cgtgccactg
    31501 aaggttttct ataatagaaa attcgacgtc tgatgctgta cacagcgcca acaattattg
    31561 gtgtccacga cgtatttgtg gtataaagcg cgccggactt ccgatccatt tcgtatacac
    31621 agactggacg gaagcgacaa tctcactttg tgtaacaaca cacacgtatc ggcacatatt
    31681 ccggggtgcc ctttggggtc ggtaatatgg gatacgtgga ggcataaccc caacttttat
    31741 atagaggttt taatcatggc aactgtttcc atgcgcgaca tgctcaaggc tggtgttcac
    31801 ttcggtcacc agacccgtta ctggaacccg aaaatgaagc cgttcatctt cggtgcgcgt
    31861 aacaaagttc acatcatcaa ccttgagaaa actgtaccga tgttcaacga agctctggct
    31921 gaactgaaca agattgtttc tcgcaaaggt aaaatccttt tcgttggtac taaacgcgct
    31981 gcaagcgaag cggtgaaaga cgctgctctg agctgcgacc agttcttcgt gaaccatcgc
    32041 tggctgggcg gtatgctgac taactggaaa accgttcgtc agtccatcaa acgtctgaaa
    32101 gacctggaaa ctcagtctca ggacggtact ttcgacaagc tgaccaagaa agaagcgctg
    32161 atgcgcactc gtgagctgga gaaactggaa aacagcctgg gcggtatcaa agacatgggc
    32221 ggtctgccgg acgctctgtt tgtaatcgat gctgaccacg aacacattgc tatcaaagaa
    32281 gcaaacaact tgggtattcc ggtatttgct atcgttgata ccaactctga tccggacggt
    32341 gttgacttcg ttatcccggg taacgacgac gcaatccgtg ctgtgaccct gtacctgggc
    32401 gctgttgctg caaccgtacg tgaaggccgt tctcaggatc tggcttccca ggcggaagaa
    32461 agcttcgtag aagctgagta ataaggcttg ataactcccc caaaatagtt cgagttgcag
    32521 aaaggcggca agctcgagaa ttcccgggag cttacatcag taagtgaccg ggatgagcga
    32581 gcgaagacaa cgcatctgcg gcgcgaaata tgaaggggga gagcccttat agaccaggta
    32641 gtacacgttt ggttaggggg cctgcatatg gccccctttt tcacttttat atctgtgcgg
    32701 tttaatgccg ggcagatcac atctccgagg attttagaat ggctgaaatt accgcatccc
    32761 tggtaaaaga gctgcgtgag cgtactggcg caggcatgat ggattgcaaa aaagcactga
    32821 ctgaagctaa cggcgacatc gagctggcaa tcgaaaacat gcgtaagtcc ggtgctatta
    32881 aagcagcgaa aaaagcaggc aacgttgctg ctgacggcgt gatcaaaacc aaaatcgacg
    32941 gcaactacgg catcattctg gaagttaact gccagactga cttcgttgca aaagacgctg
    33001 gtttccaggc gttcgcagac aaagttctgg acgcagctgt tgctggcaaa atcactgacg
    33061 ttgaagttct gaaagcacag ttcgaagaag aacgtgttgc gctggtagcg aaaattggtg
    33121 aaaacatcaa cattcgccgc gttgctgcgc tggaaggcga cgttctgggt tcttatcagc
    33181 acggtgcgcg tatcggcgtt ctggttgctg ctaaaggcgc tgacgaagag ctggttaaac
    33241 acatcgctat gcacgttgct gcaagcaagc cagaattcat caaaccagaa gacgtatccg
    33301 ctgaagtggt agaaaaagaa taccaggtac agctggatat cgcgatgcag tctggtaagc
    33361 cgaaagaaat cgcagagaaa atggttgaag gccgcatgaa gaaattcacc ggcgaagttt
    33421 ctctgaccgg tcagccgttc gttatggaac caagcaaaac tgttggtcag ctgctgaaag
    33481 agcataacgc tgaagtgact ggcttcatcc gtttcgaagt gggtgaaggc atcgagaaag
    33541 ttgagactga ctttgcagca gaagttgctg cgatgtccaa gcagtcttaa ttatcaaaaa
    33601 ggagccgcct gagggcggct tctttttgtg cccatcttgt aaattcagct aacccttgtg
    33661 gggctgcgct gaaaagcgac gtacaatgtc gctagtatta attcatttca atcgttgaca
    33721 gtctcaggaa agaaacatgg ctaccaatgc aaaacccgtc tataaacgca ttctgcttaa
    33781 gttgagtggc gaagctctgc agggcactga aggcttcggt attgatgcaa gcatactgga
    33841 tcgtatggct caggaaatca aagaactggt tgaactgggt attcaggttg gtgtggtgat
    33901 tggtgggggt aacctgttcc gtggcgctgg tctggcgaaa gcgggtatga accgcgttgt
    33961 gggcgaccac atggggatgc tggcgaccgt aatgaacggc ttggcaatgc gtgatgcact
    34021 gcaccgcgcc tatgtgaacg ctcgtctgat gtccgctatt ccattgaatg gcgtgtgcga
    34081 cagctacagc tgggcagaag ctatcagcct gttgcgcaac aaccgtgtgg tgatcctctc
    34141 cgccggtaca ggtaacccgt tctttaccac cgactcagca gcttgcctgc gtggtatcga
    34201 aattgaagcc gatgtggtgc tgaaagcaac caaagttgac ggcgtgttta ccgctgatcc
    34261 ggcgaaagat ccaaccgcaa ccatgtacga gcaactgact tacagcgaag tgctggaaaa
    34321 agagctgaaa gtcatggacc tggcggcctt cacgctggct cgtgaccata aattaccgat
    34381 tcgtgttttc aatatgaaca aaccgggtgc gctgcgccgt gtggtaatgg gtgaaaaaga
    34441 agggacttta atcacggaat aattcccgtg atggataaat aagggtaaga ttccgcgtaa
    34501 gtatcgcggg ggcgtaagtc tggttataag gcgttattgt tgcaggcagt ttggtcacgg
    34561 ccagcgcgca gcaaccggag cgtacaaaag tacgtgagga tggcgagcac tgctcggggc
    34621 caaaatggca aataaaatag cctaataatc cagacgatta cccgtaatat gtttaatcag
    34681 ggctatactt agcacacttc cactgtgtgt gactgtctgg tctgactgag acaagttttc
    34741 aaggattcgt aacgtgatta gcgatatcag aaaagatgct gaagtacgca tggacaaatg
    34801 cgtagaagcg ttcaaaaccc aaatcagcaa aatacgcacg ggtcgtgctt ctcccagcct
    34861 gctggatggc attgtcgtgg aatattacgg cacgccgacg ccgctgcgtc agctggcaag
    34921 cgtaacggta gaagattccc gtactctgaa aatcaacgtg tttgatcgtt caatgtctcc
    34981 ggccgttgaa aaagcgatta tggcctccga tctcggcctg aacccgaact ctgcgggtag
    35041 cgacatccgt gttccgctgc cgccgctgac ggaagaacgt cgtaaagatc tgaccaaaat
    35101 cgttcgtggt gaagcagaac aagcgcgtgt tgcagtacgt aatgtgcgtc gtgacgcgaa
    35161 cgacaaagtg aaagcactgt tgaaagataa agagatcagc gaagacgacg atcgccgttc
    35221 tcaggacgat gtacagaaac tgactgatgc tgcaatcaag aaaattgaag cggcgctggc
    35281 agacaaagaa gcagaactga tgcagttctg atttcttgaa cgacaaaaac gccgctcagt
    35341 agatccttgc ggatcggctg gcggcgtttt gctttttatt ctgtctcaac tctggatgtt
    35401 tcatgaagca actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg
    35461 tggtgcgcca taatcccgaa cacttccgcg tagttgcgct ggtggcaggc aaaaatgtca
    35521 ctcgcatggt agaacagtgc ctggaattct ctccccgcta tgccgtaatg gacgatgaag
    35581 cgagtgcgaa acttcttaaa acgatgctac agcaacaggg gagccgcacc gaagtcttaa
    35641 gtgggcaaca agccgcttgc gatatggcag cgcttgagga ggttgatcag gtgatggcag
    35701 ccattgttgg tgctgctggg ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca
    35761 ttttgctggc caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa
    35821 agcagagcaa agcgcaattg ttaccggtcg atagcgaaca taacgctatt tttcagagtt
    35881 taccgcaacc tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt
    35941 ccattttact taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa
    36001 caatgacgcc cgatcaagct tgccgtcatc cgaactggtc gatgggacgt aaaatttctg
    36061 tcgattcggc taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta
    36121 acgccagcgc cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg
    36181 tgcgctatca ggacggcagt gttctggcgc agctggggga accggatatg cgtacgccaa
    36241 ttgcccacac catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg ctcgattttt
    36301 gcaaactaag tgcgttgaca tttgccgcac cggattatga gcgttatcca tgcctgaaac
    36361 tggcgatgga ggcgttcgaa caaggccagg cagcgacgac agcattgaat gccgcaaacg
    36421 aaatcaccgt tgctgctttt cttgcgcaac aaatccgctt tacggatatc gctgcgttga
    36481 atttatccgt actggaaaaa atggatatgc gcgaaccaca atgtgtggac gatgtgttat
    36541 ctgttgatgc gaacgcgagt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgag
    36601 gataatccgg ctacagagag tcgcgctatt tgttagcgta gggcttcagt gatatagtct
    36661 gcgccatctg atcgtaagta gttggcttta taaggtcagg tatgccgtgg ttttacacgg
    36721 cttttttttg tataggcttc agtattcctg agtaccgtaa accctgtcag ggaataaaaa
    36781 acgcgtgatg ttgtctgcta ctcaaccact tagcgaaaaa ttgccagcgc atggctgccg
    36841 tcatgttgcg atcattatgg acggcaatgg tcgctgggca aaaaagcaag ggaagattcg
    36901 tgcctttggg cataaagccg gggcaaaatc cgtccgccgg gctgtctctt ttgcggccaa
    36961 caacggtatt gaggcgttaa cgctgtatgc ctttagtagt gaaaactgga accgaccagc
    37021 gcaggaagtc agtgcgttaa tggaactgtt tgtgtgggcg ctcgatagcg aagtaaaaag
    37081 tctgcaccga cataacgtgc gtctgcgtat tattggcgat accagtcgtt ttaactcgcg
    37141 tttgcaagaa cgtattcgta aatctgaagc gctaacagcc ggaaataccg gtctgacgct
    37201 gaatattgcg gcgaactacg gtggacgttg ggatatagtc cagggagtca ggcaactggc
    37261 tgaaaaggtg cagcaaggaa acctgcaacc agatcagata gatgaagaga tgttaaacca
    37321 gcatgtctgt atgcatgaac tggcccctgt agatttagta attaggactg ggggagagca
    37381 tcgcattagt aactttttgc tttggcaaat tgcctatgcc gaactttact ttacagatgt
    37441 tctctggccc gatttcgatg aacaagactt tgaaggggcg ttaaatgcct ttgctaatcg
    37501 agagcgtcgt ttcggcggca ccgagcccgg tgatgaaaca gcctgatggg ggtcgctttt
    37561 gctgaagtat cgcctgatat ctgcttttgt gttaataccc gtcgtcatcg cggcgttgtt
    37621 tctgttgccg ccggtggggt tcgccattgt aacgctggtg gtctgcatgc tggcagcgtg
    37681 ggaatgggga cagcttagcg gttttaccac tcgttcgcag cgagtatggt tggcggtgtt
    37741 atgcgggtta ttgttggcgc tgatgctttt tctgttgccg gaatatcacc gaaatattca
    37801 tcaaccgctg gttgaaatct cactttgggc ttcgttgggt tggtggattg tcgcgctatt
    37861 gctggtgctg ttttacccag gttccgcagc aatctggcgt aactctaaaa cattgcgcct
    37921 tatttttggc gtgctaacca ttgttccctt cttctggggc atgctggcgt tacgggcctg
    37981 gcactatgac gagaatcatt acagtggcgc aatatggctg ctctatgtca tgatcctggt
    38041 atggggcgct gactccggcg catatatgtt tggcaaattg tttggtaaac ataagctggc
    38101 accgaaggtt tctccgggta aaacctggca aggctttata ggtggactcg ctactgcagc
    38161 ggtaatctca tggggttatg gcatgtgggc gaatctcgac gtcgctcccg tcaccttact
    38221 catttgctct attgtcgcag cgttagcctc agtgctcggc gatctgaccg agagtatgtt
    38281 taagcgtgaa gcaggaatta aggacagcgg tcatttaatt ccaggacacg gtggtatttt
    38341 agatcgtatt gatagcctga cggctgcggt accggtcttt gcttgcttgt tgttactggt
    38401 attcaggacg ctttaacgga aggtaatatg ctgagttttc tctgggattt ggcttcgttc
    38461 atcgttgcac tgggtgtact tatcaccgtg catgaatttg gtcatttctg ggttgcccgg
    38521 cgttgtggtg ttcgcgttga gcgtttctca atagggtttg gtaaggcgct ctggcggcga
    38581 actgataagc tcggcaccga atatgttatc gccctgatcc cgttgggcgg ttatgtcaaa
    38641 atgctggatg agcgcgcaga accggtcgtt ccggaactcc gccaccatgc cttcaataat
    38701 aaatctgtcg gccaacgagc ggcgattatt gccgcaggtc cggttgcaaa cttcattttt
    38761 gctatctttg cctactggct ggtttttatt attggtgtgc ctggcgtacg tccggtggtt
    38821 ggcgaaatag cagccaattc gatagctgcg gaagcacaaa ttgcaccagg tacggaacta
    38881 aaagccgtag atggtatcga aacgcctgat tgggatgccg tgcgtttgca gttggtcgat
    38941 aaaattggcg atgaaagcac caccattaca gtagcgccat ttgacagcga ccaacggcgg
    39001 gatgtaaagc tcgatttacg tcactgggcg tttgagcctg ataaagaaga tccggtatct
    39061 tcgctgggga ttcgtcctcg tgggccgcaa attgaacctg tactggaaaa tgtgcagcca
    39121 aactcggcgg caagcaaggc aggtttgcaa gcaggcgaca ggatcgttaa agtcgatggt
    39181 cagcccttaa cgcagtgggt gacctttgtg atgcttgtcc gggataaccc gggtaaatcc
    39241 ttagcgttag aaatcgaaag gcaggggagt cccttgtctt tgacattaat cccggagagt
    39301 aaaccgggta atggtaaagc gattggtttt gtcggtattg agccgaaagt cattcctttg
    39361 ccagatgagt ataaagttgt acgccagtat gggccgttca acgccatcgt cgaagccacg
    39421 gacaaaacgt ggcagctgat gaagctgacg gtcagtatgc tgggaaaatt gatcaccggt
    39481 gatgtgaaac tgaacaacct cagtgggccg atctctatcg ccaagggggc tgggatgaca
    39541 gcggaactcg gggtagttta ttacctgccg tttcttgcgc ttattagcgt gaacttaggg
    39601 ataattaacc tgtttccgtt gcccgtactt gacggggggc atctgctgtt ccttgcgatc
    39661 gaaaagatca agggcggacc ggtatccgag cgggttcaag acttttgtta tcgcattggc
    39721 tcgattctgc tggtgctgtt aatggggctt gcacttttca atgatttctc tcggttatga
    39781 gagttagtta ggaagaacgc ataataacga tggcgatgaa aaagttgctc atagcgtcgc
    39841 tgctgtttag cagcgccacc gtatacggtg ctgaagggtt cgtagtgaaa gatattcatt
    39901 tcgaaggcct tcagcgtgtc gccgttggtg cggccctcct cagtatgccg gtgcgtacag
    39961 gcgacacggt taatgatgaa gatatcagta ataccattcg cgctctgttt gctaccggca
    40021 actttgagga tgttcgcgtc cttcgtgatg gtgataccct tctggttcag gtaaaagaac
    40081 gtccgaccat tgccagcatt actttctccg gtaacaaatc ggtgaaagat gacatgctga
    40141 agcaaaacct cgaggcttct ggtgtgcgtg tgggcgaatc cctcgatcgc accaccattg
    40201 ccgatatcga gaaaggtctg gaagacttct actacagcgt cggtaaatat agcgccagcg
    40261 ttaaagctgt cgtgaccccg ctgccgcgca accgtgttga cctaaaactg gtgttccagg
    40321 aaggtgtgtc agctgaaatc cagcaaatta acattgttgg taaccatgct ttcaccaccg
    40381 acgaactgat ctctcatttc caactgcgtg acgaagtgcc gtggtggaat gtggtaggcg
    40441 atcgtaaata ccagaaacag aaactggcgg gcgatcttga aaccctgcgc agctactatc
    40501 tggaccgcgg ttatgcccgt ttcaacatcg actctaccca ggtcagtctg acgccagata
    40561 aaaaaggtat ttacgtcacg gtgaacatca ccgaaggcga tcagtacaag ctttctggcg
    40621 ttgaagtgag cggcaacctt gccgggcact ccgctgaaat tgagcaactg actaagatcg
    40681 agccgggcga gctgtataac ggcactaaag tgaccaaaat ggaagatgac attaaaaagc
    40741 ttctcggtcg ctatggttat gcctatccgc gcgtacagtc gatgcctgaa attaacgatg
    40801 ccgacaaaac cgttaaatta cgcgtgaacg ttgatgcggg taaccgtttc tacgtgcgta
    40861 agatccgttt tgaaggtaac gatacctcga aagatgccgt cctgcgtcgc gaaatgcgtc
    40921 agatggaagg tgcatggttg gggagcgatc tggtcgatca gggtaaggag cgtctgaatc
    40981 gtctgggctt ctttgaaact gtcgataccg atacccaacg tgttccgggt agcccggacc
    41041 aggttgatgt agtctacaag gtgaaagaac gtaacactgg tagcttcaac ttcgggattg
    41101 gttacggtac tgaaagtggc gtgagcttcc aggctggcgt gcaacaggat aactggttag
    41161 gtacaggtta tgctgttggt atcaacggga ccaaaaacga ttaccagacc tatgctgaac
    41221 tgtcggtaac caacccgtac ttcaccgtag atggcgtaag cctcggtggt cgtctcttct
    41281 ataatgactt ccaggcagat gacgccgacc tgtccgacta taccaacaag agttatggta
    41341 cagacgtgac gttgggcttc ccgattaacg aatataactc gctgcgtgca ggtctgggtt
    41401 atgtacataa ctccctgtcc aacatgcagc ctcaggttgc gatgtggcgt tatctgtact
    41461 ctatgggtga acatccgagc acctctgatc aggataacag cttcaaaacg gacgacttca
    41521 cgttcaacta tggttggacc tataacaagc ttgaccgtgg ttacttcccg acagatggtt
    41581 cacgtgtcaa cctgaccggt aaagtgacca ttcctggatc ggataacgaa tactacaaag
    41641 tgacgttaga cacggcgact tatgtgccga tcgatgacga tcacaaatgg gttgttctgg
    41701 gtcgtacccg ctggggttat ggtgatggtt taggcggcaa agagatgccg ttctacgaga
    41761 acttctatgc cggtggttcc agcaccgtgc gtggcttcca gtccaatacc attggtccga
    41821 aagcagttta cttcccgcat caggccagta attatgatcc ggactatgat tacgaatgtg
    41881 cgactcagga cggcgcgaaa gacctgtgta aatcggatga tgctgtaggc ggtaacgcca
    41941 tggcggttgc cagcctcgag ttcatcaccc cgacgccgtt tattagcgat aagtatgcta
    42001 actcggttcg tacttcattc ttctgggata tgggtaccgt ttgggataca aactgggatt
    42061 ccagccaata ttctggatat ccggactata gtgatccaag caatatccgt atgtctgcgg
    42121 gtatcgcatt acaatggatg tccccattgg ggccgttggt gttctcctac gcccagccgt
    42181 tcaaaaagta cgatggagac aaggcagaac agttccagtt taacatcggt aaaacctggt
    42241 aagtgttctc cacaaaggaa tgtagtggta gtgtagcgat gactttaggc gatcaatata
    42301 agatcgccgg gccacgcaaa gaactgcacc ctccggtgca aatgggatgg taaggagttt
    42361 attgtgaaaa agtggttatt agctgcaggt cttggtttag cactggcaac ttctgctcag
    42421 gcggctgaca aaattgcaat cgtcaatatg ggcagcctgt tccagcaggt agcgcagaaa
    42481 accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga actgcagcgt
    42541 atggaaaccg atctgcaggc taaaatgaaa aaactgcagt ccatgaaagc gggcagcgat
    42601 cgcaccaaac tggaaaaaga cgtgatggct cagcgccaga cttttgctca gaaagcgcag
    42661 gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact ggttactcgt
    42721 atccagactg ctgtgaaatc cgttgccaac agccaggata tcgatctggt tgttgatgca
    42781 aacgccgttg cttacaacag cagcgatgta aaagacatca ctgccgacgt actgaaacag
    42841 gttaaataag taatgccttc aattcgactg gctgatttag cgcagcagtt ggatgcagaa
    42901 ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc tgcacaaaca
    42961 ggtcacatta cgttcatggt taacccaaaa taccgtgagc atttaggctt gtgccaggcg
    43021 tccgcggttg tcatgaccca ggacgatctt cctttcgcga aaagtgccgc gctggtagtg
    43081 aagaatccct acctgactta cgcgcgcatg gcgcaaattt tagataccac gccgcagccc
    43141 gcgcagaaca ttgcacccag tgcggtgatc gacgcgacgg cgaagctggg taacaacgta
    43201 tcgattggcg ctaacgcggt gattgagtcc ggcgttgaac tgggcgataa cgtgattatc
    43261 ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg tctctgggcg
    43321 aacgtaacca tttaccatga gatccagatc ggtcagaatt gcctgatcca gtccggaaca
    43381 gtggtaggcg cagacggctt tggttatgcc aacgatcgtg gtaactgggt gaagatccca
    43441 cagattggtc gcgtaattat tggcgatcgc gtggagatcg gtgcctgcac aaccatcgat
    43501 cgcggcgcgc tggatgacac tgttattggc aatggcgtga tcattgataa ccagtgccag
    43561 attgcacata acgtcgtgat tggcgacaat acggcggttg ccggtggcgt cattatggcg
    43621 ggcagcctga aaattggtcg ttactgcatg atcggcggag ccagcgtaat caacgggcat
    43681 atggaaatat gcgacaaagt gacggttacg ggcatgggta tggtgatgcg tcccatcact
    43741 gaaccaggcg tctattcctc aggcattccg ctgcaaccca acaaagtctg gcgcaaaacc
    43801 gctgcactgg tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct tgagcgcaag
    43861 gttaatcaac aagactaacg ttccatcttt tgttcgccaa actttacggc ctgtctcatt
    43921 cttacgattg cggcaggccg tgttattatt gtcgtttctt atattttgac aggaagagta
    43981 tcttgactac taacactcat actctgcaga ttgaagagat tttagaactt ctgccgcacc
    44041 gtttcccgtt cttactggtg gatcgcgtgc tggattttga agaaggtcgt tttctgcgcg
    44101 cagtaaaaaa tgtctctgtc aatgagccat tcttccaggg ccatttccct ggaaaaccga
    44161 ttttcccggg tgtgctgatt ctggaagcaa tggcacaggc aacaggtatt ctggcgttta
    44221 aaagcgtagg aaaactggaa ccgggtgagc tgtactactt cgctggtatt gacgaagcgc
    44281 gcttcaagcg cccggtcgtg cctggcgatc aaatgatcat ggaagtcact ttcgaaaaaa
    44341 cgcgccgcgg cctgacccgt tttaaagggg ttgctctggt cgatggtaaa gtagtttgcg
    44401 aagcaacgat gatgtgtgct cgtagccggg aggcctgata cgtgattgat aaatccgcct
    44461 ttgtgcatcc aaccgccatt gtggaagagg gcgcgtcaat tggcgcgaac gcacacattg
    44521 gtcctttttg tatcgttgga ccccatgtcg aaattggtga gggtaccgta ctgaaatctc
    44581 acgttgtcgt gaatggtcat actaaaattg gccgcgataa tgagatttat cagtttgcct
    44641 ccatcggcga agttaaccag gatttgaaat atgctggcga accgacccgt gtggaaatcg
    44701 gcgatcgtaa ccgcattcgc gaaagcgtca ccattcatcg tggcacagtc cagggcggtg
    44761 gattgacgaa ggtgggcagc gacaacttac tgatgatcaa cgcgcacatt gcgcacgatt
    44821 gtacggtagg taaccgctgt attctcgcca acaacgcaac gctggcgggt cacgtatcgg
    44881 ttgacgactt cgcgatcatc ggcggcatga ccgcagtcca tcagttctgc atcattggtg
    44941 cgcatgtgat ggttggcggc tgctccggtg tggcgcagga cgtccctcct tatgttattg
    45001 cgcagggtaa ccacgcaacg ccgttcggtg tcaatatcga agggctgaag cgccgcggat
    45061 tcagccgtga ggcgattacc gctatccgca atgcgtataa gctaatttat cgtagcggta
    45121 aaacgctcga tgaagtgaaa ccggaaattg ctgaactggc ggaaacatat ccggaagtga
    45181 aagcctttac cgatttcttt gcacgctcaa cgcgcggtct gattcgttaa tgactgacca
    45241 gcgtccatta acgattgccc tggtcgccgg agaaacctcc ggcgatatcc tgggggccgg
    45301 tttaatccgc gctctgaaag aacgtgtgcc caacgcccgc tttgttggtg ttgccgggcc
    45361 acgaatgcag gctgaaggct gcgaagcctg gtacgaaatg gaagaactgg cggtgatggg
    45421 cattgttgaa gtgctcggtc gtctgcgtcg cttactgcat attcgtgccg atctgacaaa
    45481 gcgttttggc gaactgaagc cagatgtttt tgttggtatt gatgcgcccg acttcaatat
    45541 tactcttgaa ggtaacctca aaaagcaggg tatcaaaacc attcattacg tcagtccatc
    45601 cgtctgggcg tggcgacaga aacgcgtttt caaaataggc agagccaccg atctggtgct
    45661 cgcatttctg cctttcgaaa aagcgtttta tgacaaatac aatgtaccgt gccgctttat
    45721 cggtcatacc atggctgatg ccatgccgtt agatccagat aaaaatggtg cccgtgatgt
    45781 gctggggatc ccttacgatg cccactgtct ggcattgttg ccgggcagcc gtggtgcaga
    45841 agtcgaaatg cttagtgccg atttcttgaa aactgcccag cttttgcgcc agacatatcc
    45901 ggatctcgaa atcgtggtgc cgctggtgaa tgccaaacgc cgcgagcagt ttgaacgcat
    45961 caaagctgaa gtcgcgccag acctgtcggt tcatttgctg gatggaatgg gccgtgaggc
    46021 gatggtcgcc agcgatgcgg cactactggc gtcggggacg gcagccctgg agtgtatgct
    46081 ggcgaaatgc ccgatggtgg tgggatatcg catgaagcct tttaccttct ggttggcgaa
    46141 gcggctggtg aaaactgatt atgtctcgct gccaaatctg ctggcgggca gagagttagt
    46201 caaagagtta ttgcaggaag agtgtgagcc gcaaaaactg gctgcggcgc tgttaccgct
    46261 gttggcgaac gggaaaacca gccacgcgat gcacgatacc ttccgtgaac tgcatcagca
    46321 gatccgctgc aatgccgatg agcaggcggc acaagccgtt ctggagttag cacaatgatc
    46381 gaatttgttt atccgcacac gcagctggtt gcgggtgtgg atgaagtcgg acgcgggccg
    46441 ttagttggcg cggtcgtcac cgctgcggtg atccttgacc cggcgcgccc gattgccggg
    46501 ctgaatgatt ccaaaaagct gagcgaaaaa cgccgtctgg cgctctatga agagatcaaa
    46561 gagaaagcgt tgagctggag tctgggccgc gcggaacccc acgaaatcga cgagctgaac
    46621 attcttcatg cgaccatgct ggcgatgcag cgtgccgtcg ctgggctgca tattgcgccg
    46681 gaatatgtgt tgattgatgg taaccgctgc ccgaaattac cgatgcctgc gatggctgtg
    46741 gtgaaagggg atagccgcgt accagaaatc agtgccgcgt ctatcctggc gaaagtgacg
    46801 cgtgacgccg aaatggcggc gctggatatt gttttcccgc aatatggttt tgcccaacat
    46861 aaagggtatc caaccgcttt tcatctggaa aaactggctg aatatggcgc gactgaacat
    46921 catcgtcgca gctttgggcc tgtcaaacgc gcactgggac ttgcgtcctg attcttgtgt
    46981 cgagattaag taaaccggaa tctgaagatg tctgaaccac gtttcgtaca cctgcgggtg
    47041 cacagcgact actcgatgat cgatggcttg gccaaaaccg caccgctggt aaaaaaggcg
    47101 gcggcgttgg gtatgccagc actggcgatc accgatttca ccaacctttg cggtctggtg
    47161 aagttctacg gagcgggaca tggcgcaggg attaagccta tcgtcggggc agattttaat
    47221 gtccagtgcg acctgctggg tgatgagtta acccacctga cggtactggc ggcgaacaat
    47281 accggctatc agaatctggc gttgctgatc tcaaaagcgt atcagcgcgg gtacggtgcc
    47341 gccgggccga tcatcgatcg cgactggctt atcgaattaa acgaagggtt gatccttctt
    47401 tccggcgggc gcatgggcga cgtcggacgc agtcttttgc gtggtaacag cgcgctggta
    47461 gatgagtgtg tcgcgtttta tgaagaacac ttcccggatc gctattttct cgagctgatc
    47521 cgcaccggca ggccggatga agaaagctat ctgcacgcgg cggtggaact ggcggaagcg
    47581 cgcggtttgc ccgtcgtggc gactaacgat gtgcgcttta tcgacagcag cgactttgac
    47641 gcacacgaaa tccgcgtcgc gatccacgac ggctttaccc tcgacgatcc taaacgcccg
    47701 cgtaactatt cgccgcagca atatatgcgt agcgaagagg agatgtgtga gctgtttgcc
    47761 gacatcccag aagcccttgc caacaccgtt gagatcgcca aacgctgtaa cgtaactgtg
    47821 cgtcttggtg aatacttcct gccgcagttc ccgaccgggg acatgagcac cgaagattat
    47881 ctggtcaagc gtgcaaaaga aggcctggaa gagcgtctgg catttttatt ccctgacgag
    47941 gaagaacgtc ttaaacgccg cccggaatat gacgaacgtc tggagactga acttcaggtt
    48001 atcaaccaga tgggcttccc gggctacttc ctcatcgtta tggaatttat ccagtggtcg
    48061 aaagataacg gcgtaccggt agggccaggc cgtggctccg gtgcgggttc actggtggcc
    48121 tacgcgctga aaatcaccga cctcgatccg ctggaatttg acctgctgtt cgaacgtttc
    48181 cttaacccgg aacgtgtctc catgcctgac ttcgacgttg acttctgtat ggagaaacgc
    48241 gatcaggtta tcgaacacgt ggcggacatg tacggtcgtg atgcggtatc gcagattatc
    48301 accttcggta cgatggcggc gaaagcggtg atccgcgacg taggccgcgt gctggggcat
    48361 ccgtacggct ttgtcgaccg tatctcgaaa ctgatcccgc ccgatccggg gatgacgctg
    48421 gcgaaagcgt ttgaagccga gccgcagctg ccggaaatct acgaagcgga tgaagaagtt
    48481 aaggcgctga tcgacatggc gcgcaaactg gaaggggtca cccgtaacgc cggtaagcac
    48541 gccggtgggg tggttatcgc gccgaccaaa attaccgatt ttgcgccgct ttattgcgat
    48601 gaagagggca aacatccggt cacccagttt gataaaagcg acgttgaata cgccggactg
    48661 gtgaagttcg acttccttgg tttgcgtacg ctcaccatca tcaactgggc gctggagatg
    48721 atcaacaagc ggcgggcgaa gaatggcgag ccaccgctgg atatcgccgc gatcccgctg
    48781 gatgacaaga aaagcttcga catgctgcaa cgctcggaaa ccacggcggt attccagctt
    48841 gaatcgcgcg gcatgaagga cctgatcaag cgtctgcaac ctgactgctt cgaagatatg
    48901 atcgcactgg tggcactgtt ccgccctggt ccgttgcagt cagggatggt ggataacttt
    48961 atcgaccgta aacatggtcg tgaagagatc tcctatccgg acgtgcagtg gcagcatgaa
    49021 agcctgaaat cggtactgga gccaacctac ggcattatct tgtatcagga acaggtcatg
    49081 cagattgccc aggtgctttc tggttatacc ctcggtggcg cggatatgct gcgccgtgcg
    49141 atgggtaaga aaaagccgga agagatggct aagcagcgtt ctgtatttgc tgaaggtgca
    49201 gaaaagaacg gaatcaacgc cgaactggcg atgaaaatct tcgacctggt ggagaaattc
    49261 gcgggttacg gatttaacaa atcgcactct gcggcctatg ctttggtgtc atatcaaacg
    49321 ttatggctga aagcgcacta tccggcggag tttatggcgg cggtaatgac cgccgatatg
    49381 gacaacaccg agaaggtggt gggcctggtg gatgagtgct ggcggatggg gctgaaaatc
    49441 ctgccaccag atataaactc cggtctttac catttccacg tcaacgacga tggcgaaatc
    49501 gtgtatggta ttggcgccat caaaggggta ggtgaaggtc cgattgaggc catcatcgaa
    49561 gcccgtaata aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg taccgacacc
    49621 aaaaagttaa accggcgggt gctggaaaaa ctgatcatgt ccggggcgtt tgaccgtctt
    49681 gggccacacc gcgcggcgct gatgaactcg ctgggtgatg cgttaaaagc ggcagatcaa
    49741 cacgcgaaag cggaagctat cggtcaggcc gatatgttcg gcgtgctggc cgaagagccg
    49801 gaacaaattg aacaatccta cgccagctgc caaccgtggc cggagcaggt ggtattagat
    49861 ggggaacgtg aaacgttagg cctgtacctg accggacacc ctatcaacca gtatttaaaa
    49921 gagattgagc gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac agaacgtggt
    49981 aaagtcatca cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac caagcgcggc
    50041 aatcgtatcg gtatctgcac gctggatgac cgttccgggc ggctggaagt gatgttgttt
    50101 actgacgccc tggataaata ccagcaattg ctggaaaaag accgcatact tatcgtcagc
    50161 ggacaggtca gctttgatga cttcagcggt gggcttaaaa tgaccgctcg cgaagtgatg
    50221 gatattgacg aagcccggga aaaatatgct cgcgggcttg ctatctcgct gacggacagg
    50281 caaattgatg accagctttt aaaccgactc cgtcagtctc tggaacccca ccgctctggg
    50341 acaattccag tacatctcta ctatcagagg gcggatgcac gcgcgcggtt gcgttttggc
    50401 gcgacgtggc gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg cctcattggt
    50461 tcggagcagg tggaactgga gtttgactaa tacaggaata ctatgagtct gaatttcctt
    50521 gattttgaac agccgattgc agagctggaa gcgaaaatcg attctctgac tgcggttagc
    50581 cgtcaggatg agaaactgga tattaacatc gatgaagaag tgcatcgtct gcgtgaaaaa
    50641 agcgtagaac tgacgcgtaa aatcttcgcc gatctcggtg catggcagat tgcgcaactg
    50701 gcacgccatc cgcagcgtcc ttataccctg gattacgttc gcctggcatt tgatgaattt
    50761 gacgaactgg ctggcgaccg tgcgtatgca gacgataaag ctatcgtcgg tggtatcgcc
    50821 cgtctcgatg gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga aaccaaagaa
    50881 aaaattcgcc gtaactttgg tatgccagcg ccagaaggtt accgcaaagc actgcgtctg
    50941 atgcaaatgg ctgaacgctt taagatgcct atcatcacct ttatcgacac cccgggggct
    51001 tatcctggcg tgggcgcaga agagcgtggt cagtctgaag ccattgcacg caacctgcgt
    51061 gaaatgtctc gccttggcgt accggtagtt tgtacggtta tcggtgaagg tggttctggc
    51121 ggtgcgctgg cgattggcgt gggcgataaa gtgaatatgc tgcaatacag cacctattcc
    51181 gttatctcgc cggaaggttg tgcgtccatt ctgtggaaga gcgccgacaa agcgccgctg
    51241 gcggctgaag cgatgggtat catcgctccg cgtctgaaag agctgaaact gatcgactcc
    51301 atcatcccgg aaccgctggg cggcgctcac cgtaacccgg aagcgatggc ggcatcgttg
    51361 aaagcgcaac tgctggcgga tctggccgat ctcgatgtgt taagcactga agatttaaaa
    51421 aatcgtcgtt atcagcgcct gatgagctac ggttacgcgt aattcgcaaa agttctgaaa
    51481 aagggtcact tcggtggccc ttttttatcg ccacggtttg agcaggctat gattaaggaa
    51541 ggattttcca ggaggaacac atgaacatca ttgccattat gggaccgcat ggcgtctttt
    51601 ataaagatga gcccatcaaa gaactggagt cggcgctggt ggcgcaaggc tttcagatta
    51661 tctggccaca aaacagcgtt gatttgctga aatttatcga acataaccct cgaatttgcg
    51721 gcgtgatttt tgactgggat gagtacagtc tcgatttatg cagcgatatc aatcaactta
    51781 atgaatatct cccgctttat gccttcatca acacccactc gacgatggat gtcagcgtgc
    51841 aggatatgcg gatggcgctc tggttttttg aatatgcgct ggggcaggcg gaagatatcg
    51901 ccattcgtat gcgtcagtac accaacgaat atcttgataa cattacgccg ccgttcacga
    51961 aagccttgtt tacctacgtc aaagagcgga agtacacctt ttgtacgccg gggcatatgg
    52021 gcggcaccgc atatcaaaaa agcccggttg gctgtctgtt ttatgatttt ttcggcggga
    52081 atacccttaa ggctgatgtc tctatttcgg tcaccgagct tggttcgttg cttgaccaca
    52141 ccgggccaca cctggaagcg gaagagtaca tcgcgcggac ttttggcgcg gaacagagtt
    52201 atatcgttac caacggaata tcgacgtcga acaaaattgt gggtatgtac gccgcgccat
    52261 ccggcagtac gctgttgatc gaccgcaact gccataaatc actggcgcat ctgttgatga
    52321 tgaacgatgt agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg attctggggg
    52381 ggatcccgcg ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct gccactacgc
    52441 aagcacaatg gccggttcat gcggtgatca ccaactccac ctatgatggt ttgctctaca
    52501 acaccgactg gatcaaacag acgctggatg tcccgtcgat ccacttcgat tccgcctggg
    52561 tgccgtatac tcattttcat ccaatctacc agggtaaaag tggtatgagc ggcgagcgtg
    52621 ttgcgggaaa agttttcttc gaaacgcaat cgacccacaa aatgctggcg gcgttatcgc
    52681 aggcttcact gatccacatt aaaggcgagt atgacgaaga ggcgtttaac gaagccttta
    52741 tgatgcatac caccacctcg cccagttatc ccattgttgc ttcggttaag acggcggcgg
    52801 cgatgctgcg tggtaatccg ggcaaacggc tgattaaccg ttcggtggag cgggcgctgc
    52861 attttcgcaa agaggtccag cggctgcggg aagagtctga cggttggttt ttcgatatct
    52921 ggcaaccgcc gcaggtggat gaagccgaat gcaggcccgt tgcgcctggc gaacaatggc
    52981 acggctttaa cgatgcggat gccgatcata tgtttctcga tccggttaaa gtcactattt
    53041 tgacaccggg gatggacgaa cagggcaata tgagtgagga ggggatcccg gcggcgctgg
    53101 tagcaaaatt cctcgacgaa cgtgggatcg tagtagagaa aaccggccct tataacctgc
    53161 tgtttctctt tagtattggc atcgataaaa ccaaagcaat gggattattg cgtgggttga
    53221 cggaatttaa gcgctcttac gatctcaacc tgcggatcaa aaatatgctg cccgatctct
    53281 atgctgaaga tcccgatttc taccgcaata tgcgtattca ggatctggcg caagggatcc
    53341 ataagctgat tcgtaaacac gatctttccg gtttgatgtt gcgggcattc gatactttgc
    53401 cggaaatgat catgacgcca catcaggcat ggcagagaca gattaaaggt gaagtagaaa
    53461 ccattgcgcc ggaacaactg gtcggtagag tatcggcaaa tatgatcctg ccttatccac
    53521 cgggcgtacc gctgttgatg ccaggagaaa tgctgaccaa agagagccgc acggtactcg
    53581 attttctact gatgctttgt tccgtagggc aacattaccc cggttttgaa acggatattc
    53641 acggcgcgaa acaggacgaa gacggcgttt accgcgtacg agtcctaaaa atggcgggat
    53701 aacttgccag agcggcttcc gggcgagtaa cgtgctgtta acaaataaag gagacgttat
    53761 gctgggttta aaacaggttc accatattgc gattattgcg acggattatg cggtgagcaa
    53821 agctttctac tgcgatattc ttggtttcac gctgcaaagc gaagtctatc gcgaagcgcg
    53881 cgactcgtgg aaaggggatt tggcgcttaa tgggcaatat gtgattgagc ttttctcttt
    53941 tccgttcccg ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc atctggcttt
    54001 tagcgttgat gacatcgatg cggcagtggc gcaccttgaa agccataacg tgaagtgtga
    54061 agccatccgt gtcgatccat acacgcaaaa acgcttcacc ttctttaacg atccggacgg
    54121 gctgccgttg gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc ccggtaacgt
    54181 gccgggcatt gctactgtaa aatcgcacca tcatgacact cacgctcaat agacaacttc
    54241 tcacctcacg ccagattctg gtggccttta gcggcgggct tgactccacc gttctgctgc
    54301 atcagttggt gcagtggcgg acggaaaatc cgggcgtcac tctgcgcgct attcatgtgc
    54361 atcacggttt aagtgccaat gccgatgcct gggttacgca ttgcgaaaac gtctgccaac
    54421 agtggcaggt gccgctggtg gtcgaacgcg tacaacttgc gcaagagggg ctggggattg
    54481 aggcccaggc gcggcaggca cgttatcagg catttgcccg taccctattg cccggcgaag
    54541 tgctggtcac cgcgcaacat ctcgacgatc aatgtgaaac ctttctgctg gcgctaaaac
    54601 gcggtagcgg ccctgccggg ctttcggcta tggcggaagt ttcggagttt gctggaacac
    54661 ggcttatccg cccgttgctt gcccgcacgc ggggggaact ggagcagtgg gcattggcgc
    54721 atggtttacg ctggattgaa gacgaaagta atcaggacga ctcatacgat cgtaactttc
    54781 tgcgcctgcg cgtagtgccg ttattgcagc agcgttggcc gcattttgcg gaagcaacgg
    54841 cccgcagcgc cacactttgt gctgaacaag agagtctgtt ggatgaactg ctggcggatg
    54901 atttagcaca ctgccaaacg tcgcagggga cgctgcagat tgcgccaatg ctggcgatga
    54961 gtgatgcccg tcgcgcggcg attattcgcc gctggctggc agggcagaat gcaccgatgc
    55021 cttcccgcga cgcgctggtg cgcatctggc aggaagtggc gctggcgcgg gaagatgctt
    55081 ccccctgttt acgtttgggc gcctttgaaa tccgccgcta tcagtcgcaa ctgtggtgga
    55141 ttaaatccgt caccgggcaa agcgaaacca ttgtgctgtg gcagacgtgg ctacaaccgc
    55201 tggaattacc ggcggggctg ggaaccgtac agcttactgc gggaggcgat attcgccctc
    55261 cgcgtgcaga cgaagcggtc agcgtgcgtt tcaaagcgcc aggattgctg catattgtcg
    55321 ggcgcaacgg cggacgtaag ctgaagaaaa tctggcaaga gttaggcgtg ccgccgtggc
    55381 tacgtgacac cacgccactg ctgttttatg gagaaacgct gattgcggcg gcaggggtat
    55441 ttgtgacgca agaaggtgtg gctgaaggtg agaatggcgt aagttttgtc tggcagaaaa
    55501 cgcttagtta agtgaaagcc ggataagacg catcaaacgt cgcatccggc gaaagtcaat
    55561 caggattcgc ttaccaccac cgtaccgatt tcagggtggc taaagctggt aattttatcc
    55621 agacgcagct cgcgggtttc gccagcggcc tcgacgacca gatactccac atttttgcgg
    55681 gagactaaat cactggcttt tgcctgcaat ttttcgccat ctttcagctc aagtgtcagc
    55741 attaaatgat gctggcaggc gagctcaaga ttatcgtaat catcacaatt aattggttga
    55801 tacgtatcat tcattgacat aatcgctcac cagtaagttt gccgcagcgt atgctgcttt
    55861 ttccctgaca gcctcagaaa gggcgtcgtc ggcagccatt tcattcagca ctttcaaaac
    55921 gcagcccagc gcgtccggaa cgtatcctaa gtctccgctg gcgatttccg cgtaccgctt
    55981 gcgtattaac tcacaatatt tttccacatg ccctcctgtc agcactctga cttaaccgtg
    56041 gatgcaagtc taagcctacg aagataaact ctgtttcgca aggtgactat accacactca
    56101 tttctgcaat atcagcgccg caactgcacg tattccgtta caatggcctc ctgattcgaa
    56161 aggagttttc ttatggcgct taaagcgaca atttataaag cgacggttaa tgtggccgat
    56221 ctcgaccgca accagtttct cgatgcctct ctgacgctgg cgcgccaccc ttcagaaacc
    56281 caggagcgta tgatgctgcg cttgctggcg tggctgaaat atgctgatga acgtctgcaa
    56341 tttacccgtg gtttgtgtgc cgatgatgag ccggaagcgt ggctgcgtaa cgatcacctg
    56401 ggcattgatt tgtggattga gctggggctg ccggatgagc gacgaattaa gaaagcctgc
    56461 acccaggccg cagaagtggc gctgtttacc tataatagtc gggcggcgca aatctggtgg
    56521 cagcaaaatc agagcaaatg tgtgcagttt gccaatcttt ccgtctggta tctggacgat
    56581 gaacaactgg cgaaagtaag cgcctttgcc gatcgtacca tgacgctgca ggcaacgatt
    56641 caggatggtg ttatctggtt atcggatgat aagaataatc tggaagttaa cttaaccgcc
    56701 tggcaacaac cttcatgatt gtgatttccc gacatgttgc tattcccgat ggtgagcttg
    56761 agatcaccgc cattcgtgcg cagggcgctg gcgggcagca tgttaataag acctcaacgg
    56821 ctattcatct gcgttttgac attcgggcgt ccagcctgcc agagtattac aaagagcgtc
    56881 tgctcgccgc cagccatcat ttgatcagca gtgatggcgt gattgtcatt aaggcacagg
    56941 aataccgcag tcaggaactg aaccgcgaag ccgctctggc ccggctggtg gcagtgatta
    57001 aagatttaac aaaagaacaa aaagcccgac gacccacgcg gcccacccgt gcatcgaaag
    57061 agcgcaggct ggcatcgaaa gcacaaaaat caagcgtgaa ggcgatgcgc ggcaaagtac
    57121 gcagcggtcg ggaataaaaa gaaggaatgg atggtgaaaa aagcgatagt gacagcgatg
    57181 gctgtaatca gcctctttac tctgatggga tgtaataatc gggccgaagt cgatacgctt
    57241 tctccggcgc aggctgccga actgaaaccg atgccgcaaa gttggcgcgg cgtgctaccg
    57301 tgtgccgatt gcgaaggaat cgaaatctct ctgttcctcg aaaaagacgg aacatgggtg
    57361 atgaatgagc gttatctcgg tgctcgtgaa gaaccttcct ccttcgcttc ctacggtaca
    57421 tgggcgcgaa ccgctgacaa gctggtatta accgacagca aaggtgaaaa gtcatattat
    57481 cgggcgaaag gcgatgcgct ggagatgctc gatcgtgaag gcaatccaat tgaatcgcag
    57541 ttcaactata cgctggaacc ggcacaatcc agtttaccta tgacgccgat gaccctgcga
    57601 ggcatgtatt tttatatggc tgatgcggcg accttcactg attgcgcgac cggaaaacgt
    57661 ttcatggtag cgaataacgc agagctggag cgtagctacc tggctgcgcg cggtcacagt
    57721 gaaaaaccgg tgttactgtc agtagaaggt cactttacgc ttgaggctaa tccggatact
    57781 ggtgcgccaa ccaaagtatt agcgcccgat acggcgggta aattttaccc gaataaggat
    57841 tgcagtagtt tggggcagta aacctgctta aggcagaaac aaacgcaaaa ttgcctgatg
    57901 cgctgcgctt atcaggccta cagcatataa tgcaacattt tgaattagca cgattttgta
    57961 ggccggataa ggcgtttacg ccgcatccgg cattttgtgc acgaggctgg ctttatcact
    58021 gagtcagccc aacaaagcga cccgccttga tataaatccc aggcttcagg tgcccaacat
    58081 actgtaactg cgtttctggc ttaaacgcag agacatcacc aatacgcata tgcatcagat
    58141 cggcagggct aatccagccc gattgcgcca gtgtaagtgg atgcccggat ttggcaaatg
    58201 catcagtgac aaattccgaa caaaaccacg actttttgtc tccttcgcac acactgctta
    58261 actgcgcttt cgccaggtcg ctgacgcact gttggcgaaa atcctcggag aacggattca
    58321 gtgagcacat ctggcgagtc accataaagg gaataaattc gacaatgccg cgatagttat
    58381 aaccgctatc tttgatttta ttggcaaacg cggtgatatc tgtggcttgt tgcggggtaa
    58441 gatccgggac tcgtaagacg aaaagcttat cactatgctt catcgctttt ttaagggaaa
    58501 caatctggac gccagcgcct gtcgcttctg caacgttatt atcacccaga aagattgcaa
    58561 cgtgactcac agaggaagtg ctgaagacgc ggattccaaa tgaggttacc ccaaggcttg
    58621 aggagaacag caaatcgccg ggtttgagat caggtgccgt tatttctttt attgattgtt
    58681 cggtgaaaga gctttgatgc tggaatttga cagcccatgt ttttgcctcg gcatctactg
    58741 ctgtggctga tgggtctggc tggctgatat caacggtaca agccgaaagt aaaagaaagc
    58801 tggggagaag caggcggcag tacgcctttg gtttatccat tttatacaat ccatgtaaaa
    58861 aagggccctg aaattcagga ccctttctgg catcagcctt taatctgttt caccagatat
    58921 tcgacgatgt caccagtctt aattaactgt ttctcgccgt tgcgacgata tttatattcg
    58981 atatcgtcgt tgtcgaggtt acggtcgccc agcacaatag tgtgcggaat accgatcagt
    59041 tccatatcag caaacatcac gcccggacgc tctttgcggt catccagcag cacttcgata
    59101 ccttgtgcac gcagttcgct gtacagtttc tcagcaagct cttgtacgcg gaaggatttg
    59161 tgcatgttca tcggcagaat cgccacctgg aacggcgcga tagcgtcagg ccatacgatg
    59221 ccgcgttcgt cgtagttctg ctcaatcgcc gcagctacca cacgcgttac cccgataccg
    59281 tagcaaccca tcgtcaggat ttggttacgg ccatcttcac cctgtacgga ggctttcagt
    59341 gcttcggagt acttggtacc cagctggaag atgtgaccaa cttcgatacc acgtttgatc
    59401 agcagcgtac cctggccatc cgggcttgga tcgccagcca ccacgttacg gatatctgca
    59461 acttccgggg tagcgacatc gcgatcccag ttgataccga agtagtgttt accatcgatg
    59521 ttagcaccag cagcgaaatc actcatcgcc gcaacggtac ggtcaatcac caccggaatc
    59581 ggcatgttta ccggacccag tgaacccgga ccggctttaa ccacagcacg aatttcttct
    59641 tcggtcgcga aagtcagcgg gcttgcaacc tgcggcagtt tttctgcttt aacttcgttc
    59701 agctcgtgat caccgcgcac cagcagcgca accagcggga agctactgcc ttcaaccgct
    59761 ttaaccagca gagtcttaac cgttttctca atcggcagat tgaactgttc aaccagttcc
    59821 gcgatggttt tcgcgttcgg cgtatcaacc agcgtcattt cctgggtagc agcagcgcgc
    59881 ggttctttcg gcgcgatagc ttctgccagt tcaatgttcg ctgcatagtc agaggtgtcg
    59941 gagaagacca catcgtcttc accgctctgc gccagcacct ggaattcgtg agaggcgctg
    60001 ccgccgatag aaccggtgtc ggcctgtacg gcgcggaaat ccagccccat gcggctgaag
    60061 attttgctgt aggccgcata cattgcatcg taggtttcct gcagggattc ctgagaagta
    60121 tggaaagagt aagcatcttt catcaggaat tcgcgggaac gcatgacgcc gaaacgcgga
    60181 cgcacttcgt cgcggaactt ggtctggatc tgatagaagt tcagcggcag ctgtttgtaa
    60241 gagctaagct cgttacgaat caggtcagtg ataacttctt catgagttgg gccgagtacg
    60301 aacggacgct cgccacggtc aacaaaacgc agcagttccg gaccgtactg ttcccaacga
    60361 ccactctctt gccacaaatc ggctggctga accaccggca tcgacacctc gatcgcaccg
    60421 gcgttgttca tctcttcacg cacgatgttt tcgacttttt tcagaacgcg cacgccggtc
    60481 ggcagccagg tatataaccc ggaggccagc ttgcggatca tcccggcgcg cagcatcagc
    60541 tgatggctga tcacctcggc gtcggcaggt gtctccttga gagtggagag caggtattgg
    60601 ctagtacgca tgttgttacg gttccagttg gaaggtagaa caggctcaag gcgagcctgg
    60661 gacaaaaaaa gtgatttagt ttaccagtgc aaaagaaatg tcaaaagaga agggcgtgaa
    60721 tttaacgcgg ttccagcgca aagacttcaa aacctgcgtc ggtgacgcgc cagcgaacgt
    60781 taaaatcatg tagccagacg gcataggttt tgcccgtttc ctcaccttta cgataggccg
    60841 ggcgcgggtc ctgcgccagt acttcgcgga taaacagcgt taactgcgga taacgcttct
    60901 ccagcgtcaa aagctgcttt tcgacctctg cggtaaaact caccgccatc tctgcagctg
    60961 gcgcactttg cgcatagctg gcactggcat cgggaagcga ttcggcaaag gggagatacg
    61021 gtttgatatc cactaccggc gtaccatcga ccagatccag actgccgagc ttcagaatca
    61081 cgctgccttt atggcaaaca acctctttca gctctaccag cgacatgcca attgggttag
    61141 ggcggaaagt agagcgtgtt gcgaaaaccc ccattctggc gttaccgccg aggcgcggtg
    61201 gacgcacagt cggacgccag ccgccttcca tcgtttgatg aaagacgaaa aggatccata
    61261 aatggctgaa tgcttccagg ccgcgaacgg cgtcggcctg gttgtaggaa gctatgagat
    61321 gcagttcgcc gttggcgctt tttaccagac ctggctggcg cggaacggcg aatttttctt
    61381 tatagggcga gcgaataacg cctatttgct cgaactggaa actgctcatt tcgccgtaat
    61441 gttaagcgca gagccgatac atacagcctg acgatagcag cccggcgtac cgctggtgac
    61501 ttcgcagcta tgcagtaata cagcattggc tttcatttta gaggcattga tttgcatctg
    61561 cttacgtgcg gttggaatgc tcggcggaga gtcctgatta gaggcctggc aagagtcgcc
    61621 actgacttca ccgagatcgc ggaacggttt gccgactaat tcttctgcat tggtataaat
    61681 tcggaccggc gtggcgcgcg gcgctttcgg ttttgcaggc tccgctttcg gctggggtgc
    61741 agtgctttga acgggttcga caggggatct gcttaacatg gaacagccgc ttagcatgag
    61801 tgctactaaa cagatcggta aagcacgcat agtatttcct caatgtatga tcaaaacgtc
    61861 aatattgaat caggagcttg taaaaatgac aagacgggca agcgcccgtc ctgaatgata
    61921 ttacaaattg tggaaacagc ctaaaaatta ccagccttta acagcgccgc cgttaaacac
    61981 tttgtttgct gcttcgtaaa cttcatcaga ctgataagcc tggacgaatt tcttcacatt
    62041 ctcggcgtct ttgttatctt cacgcgttac gatcaggttt acgtatgggg agtctttatc
    62101 ttcgacaaag ataccgtctt tcgccggagt caggccaatc tggctggcat aggtggtatt
    62161 gataactgcc agagcgattt gcgcgtcgtc cagagaacgc ggcagttgcg gtgcttccag
    62221 ttcaacaatt ttcagatttt ttgggttctc aacaacatca agaacggtcg gcagcaggcc
    62281 aacgccatct ttcagtttga tcaagcccac tttttgcagc agcagcagtg aacgaccaag
    62341 attagttgga tcgtttggca cggcaacctg cgaaccatcc tgcagttcat ccagtgattt
    62401 gattttcttg gagtaaccag caatcggata aacaaaagtg ttgcctactg cgaccagttt
    62461 gtagccacga tctttcagtt gctgatcaag gtacggttta tgctggaagg cgttggcgtc
    62521 gatatcgcct ttgctcaatg cttcgtttgg cagaacatag tcgttgaagg ttaccagctc
    62581 aacgtccagg ccatatttgt ctttcgcaac tttctgcgcg acttctgcta cctgctgttc
    62641 ggcaccaaca atcacgccga ctttaatgtg gtttggatct ttttcatcct gaccgcagcc
    62701 taccagtgcc agtgatccaa tcagggctcc cactgccgca aaggttttga atttgaacgc
    62761 cataccttat tccttcttca attatttatg ttgtgttgaa cgttacttgc gagtgacagc
    62821 ccggacgatg cggtcgcctg caaactgaat taaataaacc agaatgacca gcaataccag
    62881 taccgtattc atcaccgtcg cgttgtagcc gatgtagcca tactgatagc caatctgacc
    62941 taaaccaccg gcaccgactg caccacccat cgcggaataa ccgaccaggg taatcagggt
    63001 gatagttgcc gcattcacca gacccggcaa cgcttccggt aacagcactt tacagacgat
    63061 ctgcattggc gtggcaccca ttgcgcggga agcttcaatt aacccggttg ggatctccag
    63121 cagagcgttc tcgaccatac gggcaataaa cggtgctgca ccaacggtta acggaacaat
    63181 tgctgcctgc aaaccaatcg atgtaccgac aataacgcgg gtaaacggaa tcatccatac
    63241 cagcaagata atgaacggga tggaacggaa aatgttcaca atcgcagaaa cggtacgata
    63301 cagcttcgcg ttagcaataa tttgccccgg acgcgtgaca taaagcagaa cgccaaccgg
    63361 cagaccaatc acaaacccaa aaaatccgga tacgaaggtc attgccagcg tttcccatac
    63421 gccacgaacc agcagccaca tcatcggctc agacataacc cagtacctct acttttacat
    63481 ggtgttcctg cagccaggca atggcggctt gcgtatcttg ttgtgtgccg tgcatttcag
    63541 tcagcatgat gccgaattta acgccaccgg cgtagtccat ctgcgcgcta ataatgttgt
    63601 tgttgacgtt gaaacgacgc gcggtttcag aaagcagtgg ggcatcgacc gattgaccgg
    63661 taaactccag acgcagcatc ggcacgcagt cagtaaatgg ctccgtttgc agacgttcct
    63721 ggtaatcttc cgggatatcc agatgcaggg tcgactgaat aaacttctgc gccagcggcg
    63781 ttttcggatg cgagaacact tcacttaccg tgtcctgctc gatcagttcg ccattgctga
    63841 tgaccgccac gcaatcacaa atacgcttca caacgtccat ttcgtgggtg atcaacagaa
    63901 tcgtcaaacc cagacggcgg ttaatgtctt tcagcagttc gagaatagaa cgtgtcgttg
    63961 ccgggtccag cgcgctggtg gcttcatcac acagcaatac tttgggattg ctggctaatg
    64021 cacgggcaat cgccacacgt tgtttctgcc caccggaaag attcgacggg tagctatcat
    64081 gcttatcgcc aagaccaacc aatgacagca attccgtcac gcgacgtttg atctcgtctt
    64141 tcggtgtgtt gtccagctcc agcggcagag ccacgttgcc aaaaacagta cgcgaagaga
    64201 gcaggttaaa atgctggaaa atcataccaa tctggcggcg agctttggtc aactcggatt
    64261 ctgacagcgt ggtcaattcc tggccatcga ccagcacgct accctcggtt gggcgctcca
    64321 gcaagtttac acaacgtata agcgtactct tacccgcgcc tgaggcaccg ataacgccat
    64381 aaatttgtcc agctggcaca tgcaggctga cgttgttcaa cgcctggatg gtgcgggtgc
    64441 cctggtggaa cactttggtg atattcgaaa gttttatcat tgattattta ttatcgtcat
    64501 taagttagtc gtagcatctc gaatgcctga aacgggcaac gccgtcaatg aaatggatgt
    64561 taaggcatcc agacgtctaa atcaatcagg tttatgcgaa gagcactttc ttgcaggtcg
    64621 aaacatgcga tactagcgtc ccatgcctta ttaaggagct ataaaaggtg gcgaagagcg
    64681 tacccgcaat ttttcttgac cgtgatggca ccattaatgt cgatcacggc tatgtccatg
    64741 agatcgacaa ctttgaattt atcgacggtg ttattgacgc catgcgcgag ctaaaaaaaa
    64801 tgggctttgc gctggtggta gtaaccaacc agtctggcat tgctcgcggt aaatttaccg
    64861 aagcacagtt tgaaacgctg accgagtgga tggactggtc gctggcggac cgagatgtcg
    64921 atctggatgg tatctattat tgcccgcatc atccgcaggg gagtgttgaa gagtttcgcc
    64981 aggtctgcga ttgccgcaaa ccacatccgg ggatgctttt gtcagcacgc gattatttgc
    65041 atattgatat ggccgcttct tatatggtgg gcgataaatt agaagatatg caggcagcgg
    65101 ttgcggcgaa cgtgggaaca aaagtgctgg tgcgtacggg taaacctatt acacctgaag
    65161 cagaaaacgc ggcagattgg gtgttaaata gcctggcaga cctgccgcaa gcgataaaaa
    65221 agcagcaaaa accggcgcaa tgattaaaag atgaacggtt gaaataaaaa tgcatttttc
    65281 cgcttgtctt cctgagccga ctccctataa tgcgcttcca tcgacacggc ggatgtgaat
    65341 cacttcacac aaacagccgg ttcggttgaa gagaaaaaat cctgaaattc agggttgact
    65401 ctgaaatagg aaagtgtaat atacgccacc tcgcgacagt gagctgaaag ctgcgtcgca
    65461 actgctcttt aacaatttat cagacaatct gtgtgggcac tcgaagatac ggattcttaa
    65521 cgtcgcaaga cgataaatga ataccaagtc tcaagagtga acacgtaatt cattacgaag
    65581 tttaattctt tgagcatcaa acttttaaat tgaagagttt gatcatggct cagattgaac
    65641 gctggcggca ggcctaacac atgcaagtcg aacggtaaca ggaagcgctt gctgcttcgc
    65701 tgacgagtgg cggacgggtg agtaatgtct gggaaactgc ctgatggagg gggataacta
    65761 ctggaaacgg tagctaatac cgcataacgt cgcaagacca aagaggggga ccttcgggcc
    65821 tcttgccatc ggatgtgccc agatgggatt agcttgttgg tggggta
//
LOCUS       DAZSNO010000004        60419 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZSNO010000004 DAZSNO010000000
VERSION     DAZSNO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.004"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..322
                     /locus_tag="ACHWN2_000218"
     CDS             <1..322
                     /locus_tag="ACHWN2_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8929921.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="ACHWN2_000219"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="ACHWN2_000219"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR8929922.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="ACHWN2_000220"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="ACHWN2_000220"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR8929923.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHWN2_000221"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHWN2_000221"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR8929924.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="ACHWN2_000222"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="ACHWN2_000222"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR8929925.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="ACHWN2_000223"
     CDS             complement(3726..4955)
                     /locus_tag="ACHWN2_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR8929926.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="ACHWN2_000224"
     CDS             complement(5019..5459)
                     /locus_tag="ACHWN2_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR8929927.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="ACHWN2_000225"
     CDS             complement(5564..6334)
                     /locus_tag="ACHWN2_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8929928.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="ACHWN2_000226"
     CDS             complement(6331..7257)
                     /locus_tag="ACHWN2_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8929929.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="ACHWN2_000227"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="ACHWN2_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR8929930.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHWN2_000228"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHWN2_000228"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR8929931.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="ACHWN2_000229"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="ACHWN2_000229"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR8929932.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHWN2_000230"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHWN2_000230"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR8929933.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="ACHWN2_000231"
     CDS             12964..13311
                     /locus_tag="ACHWN2_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR8929934.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="ACHWN2_000232"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="ACHWN2_000232"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR8929935.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="ACHWN2_000233"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="ACHWN2_000233"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR8929936.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHWN2_000234"
     CDS             complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHWN2_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR8929937.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHWN2_000235"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHWN2_000235"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR8929938.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="ACHWN2_000236"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="ACHWN2_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHWN2_000237"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHWN2_000237"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR8929939.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHWN2_000238"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHWN2_000238"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR8929940.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHWN2_000239"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHWN2_000239"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR8929941.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHWN2_000240"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHWN2_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR8929942.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="ACHWN2_000241"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="ACHWN2_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR8929943.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHWN2_000242"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHWN2_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR8929944.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHWN2_000243"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHWN2_000243"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR8929945.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="ACHWN2_000244"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="ACHWN2_000244"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR8929946.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHWN2_000245"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHWN2_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR8929947.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /locus_tag="ACHWN2_000246"
                     /pseudo
     CDS             32769..33916
                     /locus_tag="ACHWN2_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="ACHWN2_000247"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="ACHWN2_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR8929948.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHWN2_000248"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHWN2_000248"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR8929949.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="ACHWN2_000249"
                     /pseudo
     CDS             complement(36348..36386)
                     /locus_tag="ACHWN2_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="ACHWN2_000250"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="ACHWN2_000250"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR8929950.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="ACHWN2_000251"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="ACHWN2_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR8929951.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="ACHWN2_000252"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="ACHWN2_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR8929952.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHWN2_000253"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHWN2_000253"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR8929953.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHWN2_000254"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHWN2_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR8929954.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHWN2_000255"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHWN2_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR8929955.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHWN2_000256"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHWN2_000256"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR8929956.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHWN2_000257"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHWN2_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR8929957.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHWN2_000258"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHWN2_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR8929958.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHWN2_000259"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHWN2_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR8929959.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHWN2_000260"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHWN2_000260"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR8929960.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHWN2_000261"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHWN2_000261"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR8929961.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHWN2_000262"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHWN2_000262"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR8929962.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHWN2_000263"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHWN2_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR8929963.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHWN2_000264"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHWN2_000264"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR8929964.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHWN2_000265"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHWN2_000265"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR8929965.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHWN2_000266"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHWN2_000266"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR8929966.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHWN2_000267"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHWN2_000267"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR8929967.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHWN2_000268"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHWN2_000268"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR8929968.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHWN2_000269"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHWN2_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR8929969.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHWN2_000270"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHWN2_000270"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR8929970.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHWN2_000271"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHWN2_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR8929971.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14472 a        16732 c        14850 g        14365 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gcaccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAZSNO010000005        59981 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZSNO010000005 DAZSNO010000000
VERSION     DAZSNO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59981
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.005"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACHWN2_000272"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACHWN2_000272"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR8929972.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACHWN2_000273"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACHWN2_000273"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR8929973.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACHWN2_000274"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACHWN2_000274"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR8929974.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACHWN2_000275"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACHWN2_000275"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR8929975.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACHWN2_000276"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACHWN2_000276"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR8929976.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACHWN2_000277"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACHWN2_000277"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR8929977.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="ACHWN2_000278"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="ACHWN2_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR8929978.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACHWN2_000279"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACHWN2_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR8929979.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACHWN2_000280"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACHWN2_000280"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR8929980.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACHWN2_000281"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACHWN2_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR8929981.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACHWN2_000282"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACHWN2_000282"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR8929982.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="ACHWN2_000283"
     CDS             complement(15665..16702)
                     /locus_tag="ACHWN2_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR8929983.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="ACHWN2_000284"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="ACHWN2_000284"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR8929984.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACHWN2_000285"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACHWN2_000285"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR8929985.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="ACHWN2_000286"
     CDS             19444..19884
                     /locus_tag="ACHWN2_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR8929986.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACHWN2_000287"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACHWN2_000287"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR8929987.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21544)
                     /locus_tag="ACHWN2_000288"
                     /pseudo
     CDS             complement(20611..21544)
                     /locus_tag="ACHWN2_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACHWN2_000289"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACHWN2_000289"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR8929988.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACHWN2_000290"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACHWN2_000290"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR8929989.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACHWN2_000291"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACHWN2_000291"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFR8929990.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACHWN2_000292"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACHWN2_000292"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR8929991.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACHWN2_000293"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACHWN2_000293"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR8929992.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACHWN2_000294"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACHWN2_000294"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR8929993.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRTQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACHWN2_000295"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACHWN2_000295"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR8929994.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACHWN2_000296"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACHWN2_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR8929995.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="ACHWN2_000297"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="ACHWN2_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR8929996.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="ACHWN2_000298"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="ACHWN2_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR8929997.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="ACHWN2_000299"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="ACHWN2_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR8929998.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="ACHWN2_000300"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="ACHWN2_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR8929999.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVTQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="ACHWN2_000301"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="ACHWN2_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR8930000.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="ACHWN2_000302"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="ACHWN2_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR8930001.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="ACHWN2_000303"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="ACHWN2_000303"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR8930002.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="ACHWN2_000304"
     CDS             37412..37681
                     /locus_tag="ACHWN2_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR8930003.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="ACHWN2_000305"
     CDS             complement(37684..38043)
                     /locus_tag="ACHWN2_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR8930004.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="ACHWN2_000306"
     CDS             38184..38810
                     /locus_tag="ACHWN2_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR8930005.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="ACHWN2_000307"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="ACHWN2_000307"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR8930006.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="ACHWN2_000308"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="ACHWN2_000308"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR8930007.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="ACHWN2_000309"
     CDS             41650..42315
                     /locus_tag="ACHWN2_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR8930008.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="ACHWN2_000310"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="ACHWN2_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR8930009.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="ACHWN2_000311"
     CDS             complement(42949..44166)
                     /locus_tag="ACHWN2_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8930010.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="ACHWN2_000312"
     CDS             44300..45349
                     /locus_tag="ACHWN2_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR8930011.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="ACHWN2_000313"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="ACHWN2_000313"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR8930012.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="ACHWN2_000314"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="ACHWN2_000314"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8930013.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="ACHWN2_000315"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="ACHWN2_000315"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR8930014.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="ACHWN2_000316"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="ACHWN2_000316"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR8930015.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="ACHWN2_000317"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="ACHWN2_000317"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR8930016.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="ACHWN2_000318"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="ACHWN2_000318"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR8930017.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="ACHWN2_000319"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="ACHWN2_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR8930018.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="ACHWN2_000320"
     CDS             complement(51544..51903)
                     /locus_tag="ACHWN2_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR8930019.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="ACHWN2_000321"
     CDS             complement(51900..52175)
                     /locus_tag="ACHWN2_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR8930020.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="ACHWN2_000322"
     CDS             complement(52234..53358)
                     /locus_tag="ACHWN2_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8930021.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="ACHWN2_000323"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="ACHWN2_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR8930022.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="ACHWN2_000324"
     CDS             complement(56090..57157)
                     /locus_tag="ACHWN2_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR8930023.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57220..57333)
                     /gene="yhiY"
                     /locus_tag="ACHWN2_000325"
     CDS             complement(57220..57333)
                     /gene="yhiY"
                     /locus_tag="ACHWN2_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR8930024.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(57523..59145)
                     /locus_tag="ACHWN2_000326"
     CDS             complement(57523..59145)
                     /locus_tag="ACHWN2_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR8930025.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..>59981)
                     /locus_tag="ACHWN2_000327"
     CDS             complement(59407..>59981)
                     /locus_tag="ACHWN2_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR8930026.1"
                     /translation="VLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSET
                     KVLTSHHGIIRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHL
                     IRSNAIAPTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDN
                     YTMGLNSWKIAPNERGDKKGVPGLSCFQPQ"
BASE COUNT        14184 a        16499 c        15168 g        14130 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcagcacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg aagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaaaatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcgtg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcggg aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag cccttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 tcacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat cagattaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aacagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtaca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgtc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctgaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc acgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatca cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttc cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caggcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcgctt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggccggaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcctggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatt
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaatatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaat aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg a
//
LOCUS       DAZSNO010000006        59859 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZSNO010000006 DAZSNO010000000
VERSION     DAZSNO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59859
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.006"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="ACHWN2_000328"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="ACHWN2_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR8930027.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="ACHWN2_000329"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="ACHWN2_000329"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR8930028.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="ACHWN2_000330"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="ACHWN2_000330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR8930029.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACHWN2_000331"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACHWN2_000331"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR8930030.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACHWN2_000332"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACHWN2_000332"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR8930031.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="ACHWN2_000333"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="ACHWN2_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR8930032.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="ACHWN2_000334"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="ACHWN2_000334"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR8930033.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="ACHWN2_000335"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="ACHWN2_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="ACHWN2_000336"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="ACHWN2_000336"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR8930034.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="ACHWN2_000337"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="ACHWN2_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR8930035.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACHWN2_000338"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACHWN2_000338"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR8930036.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="ACHWN2_000339"
     CDS             complement(13538..13933)
                     /locus_tag="ACHWN2_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR8930037.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="ACHWN2_000340"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="ACHWN2_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR8930038.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="ACHWN2_000341"
     CDS             complement(15220..15447)
                     /locus_tag="ACHWN2_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR8930039.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="ACHWN2_000342"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="ACHWN2_000342"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR8930040.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="ACHWN2_000343"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="ACHWN2_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR8930041.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="ACHWN2_000344"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="ACHWN2_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR8930042.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="ACHWN2_000345"
     CDS             complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="ACHWN2_000345"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR8930043.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFVEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19688..19764
                     /locus_tag="ACHWN2_000346"
     tRNA            19688..19764
                     /locus_tag="ACHWN2_000346"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19722..19724,aa:Met,seq:cat)
     gene            19798..19874
                     /locus_tag="ACHWN2_000347"
     tRNA            19798..19874
                     /locus_tag="ACHWN2_000347"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19832..19834,aa:Met,seq:cat)
     gene            complement(19949..21202)
                     /gene="amiC"
                     /locus_tag="ACHWN2_000348"
     CDS             complement(19949..21202)
                     /gene="amiC"
                     /locus_tag="ACHWN2_000348"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR8930044.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21434..22765
                     /gene="argA"
                     /locus_tag="ACHWN2_000349"
     CDS             21434..22765
                     /gene="argA"
                     /locus_tag="ACHWN2_000349"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR8930045.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22827..24653)
                     /gene="recD"
                     /locus_tag="ACHWN2_000350"
     CDS             complement(22827..24653)
                     /gene="recD"
                     /locus_tag="ACHWN2_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR8930046.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24653..28195)
                     /gene="recB"
                     /locus_tag="ACHWN2_000351"
     CDS             complement(24653..28195)
                     /gene="recB"
                     /locus_tag="ACHWN2_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR8930047.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28188..31076)
                     /gene="ptrA"
                     /locus_tag="ACHWN2_000352"
     CDS             complement(28188..31076)
                     /gene="ptrA"
                     /locus_tag="ACHWN2_000352"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR8930048.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31252..34620)
                     /gene="recC"
                     /locus_tag="ACHWN2_000353"
     CDS             complement(31252..34620)
                     /gene="recC"
                     /locus_tag="ACHWN2_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR8930049.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34633..34983)
                     /locus_tag="ACHWN2_000354"
     CDS             complement(34633..34983)
                     /locus_tag="ACHWN2_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR8930050.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34968..35375)
                     /locus_tag="ACHWN2_000355"
     CDS             complement(34968..35375)
                     /locus_tag="ACHWN2_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR8930051.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35372..35935)
                     /locus_tag="ACHWN2_000356"
     CDS             complement(35372..35935)
                     /locus_tag="ACHWN2_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR8930052.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35926..36396)
                     /locus_tag="ACHWN2_000357"
                     /pseudo
     CDS             complement(35926..36396)
                     /locus_tag="ACHWN2_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36580..37374)
                     /gene="thyA"
                     /locus_tag="ACHWN2_000358"
     CDS             complement(36580..37374)
                     /gene="thyA"
                     /locus_tag="ACHWN2_000358"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR8930053.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37381..38256)
                     /gene="lgt"
                     /locus_tag="ACHWN2_000359"
     CDS             complement(37381..38256)
                     /gene="lgt"
                     /locus_tag="ACHWN2_000359"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR8930054.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38407..40653)
                     /gene="ptsP"
                     /locus_tag="ACHWN2_000360"
     CDS             complement(38407..40653)
                     /gene="ptsP"
                     /locus_tag="ACHWN2_000360"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR8930055.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40666..41196)
                     /gene="rppH"
                     /locus_tag="ACHWN2_000361"
     CDS             complement(40666..41196)
                     /gene="rppH"
                     /locus_tag="ACHWN2_000361"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR8930056.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(41549..41695)
                     /gene="ygdT"
                     /locus_tag="ACHWN2_000362"
     CDS             complement(41549..41695)
                     /gene="ygdT"
                     /locus_tag="ACHWN2_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR8930057.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     HGIG"
     gene            41881..42570
                     /gene="mutH"
                     /locus_tag="ACHWN2_000363"
     CDS             41881..42570
                     /gene="mutH"
                     /locus_tag="ACHWN2_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR8930058.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42639..43352
                     /locus_tag="ACHWN2_000364"
     CDS             42639..43352
                     /locus_tag="ACHWN2_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR8930059.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43490..43708
                     /gene="ygdR"
                     /locus_tag="ACHWN2_000365"
     CDS             43490..43708
                     /gene="ygdR"
                     /locus_tag="ACHWN2_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR8930060.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43816..44856
                     /locus_tag="ACHWN2_000366"
     CDS             43816..44856
                     /locus_tag="ACHWN2_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR8930061.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44889..46079)
                     /gene="lplT"
                     /locus_tag="ACHWN2_000367"
     CDS             complement(44889..46079)
                     /gene="lplT"
                     /locus_tag="ACHWN2_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR8930062.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46072..48231)
                     /gene="aas"
                     /locus_tag="ACHWN2_000368"
     CDS             complement(46072..48231)
                     /gene="aas"
                     /locus_tag="ACHWN2_000368"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR8930063.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48817..49848
                     /gene="galR"
                     /locus_tag="ACHWN2_000369"
     CDS             48817..49848
                     /gene="galR"
                     /locus_tag="ACHWN2_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR8930064.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49855..51117)
                     /gene="lysA"
                     /locus_tag="ACHWN2_000370"
     CDS             complement(49855..51117)
                     /gene="lysA"
                     /locus_tag="ACHWN2_000370"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR8930065.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51239..52174
                     /gene="lysR"
                     /locus_tag="ACHWN2_000371"
     CDS             51239..52174
                     /gene="lysR"
                     /locus_tag="ACHWN2_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR8930066.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52161..52853)
                     /gene="ygeA"
                     /locus_tag="ACHWN2_000372"
     CDS             complement(52161..52853)
                     /gene="ygeA"
                     /locus_tag="ACHWN2_000372"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR8930067.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52982..54400)
                     /gene="araE"
                     /locus_tag="ACHWN2_000373"
     CDS             complement(52982..54400)
                     /gene="araE"
                     /locus_tag="ACHWN2_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR8930068.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54715..55476)
                     /gene="kduD"
                     /locus_tag="ACHWN2_000374"
     CDS             complement(54715..55476)
                     /gene="kduD"
                     /locus_tag="ACHWN2_000374"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR8930069.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55506..56342)
                     /gene="kduI"
                     /locus_tag="ACHWN2_000375"
     CDS             complement(55506..56342)
                     /gene="kduI"
                     /locus_tag="ACHWN2_000375"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR8930070.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56629..57810)
                     /locus_tag="ACHWN2_000376"
     CDS             complement(56629..57810)
                     /locus_tag="ACHWN2_000376"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR8930071.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58065..59294
                     /locus_tag="ACHWN2_000377"
                     /pseudo
     CDS             58065..59294
                     /locus_tag="ACHWN2_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            59721..>59859
                     /locus_tag="ACHWN2_000378"
     CDS             59721..>59859
                     /locus_tag="ACHWN2_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8930072.1"
                     /translation="MQLYTDREVLNMGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCA
                     NS"
BASE COUNT        14780 a        16062 c        14575 g        14442 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 cgggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttctaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggct gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgccgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcaggggac ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcccctgtca tatccgtaaa
    18361 aacggcatac agaatatcac atcagccgct aaagacgtta cctgcgcccg gagccgtttt
    18421 cagcacccaa acccgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc
    18481 gatcccttga tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac
    18541 catcagacgc aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc
    18601 tgccagcaag gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc
    18661 aaccagcggc accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac
    18721 gaaagacggg ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca
    18781 atgacgaatc gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac
    18841 cttaccaatg ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg
    18901 actgccatca ccaaagtcga tatacccact accctgcaca tccataatga agttatccat
    18961 cagagagttg ctgtaagcga ggatgtattt atcgcccaat gccccggcgt agatctccgc
    19021 acgagacggc agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc
    19081 ctggcgggta tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc
    19141 atagttgtcg gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt
    19201 atcaccgcct gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata
    19261 cagacgcggt gacgaattac ggatatggtt aatttgctcg acaaaatcac cggcgttaat
    19321 cggcgcgcca acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc
    19381 tttatattgc tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac
    19441 cgtgcccata agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa
    19501 aaataaggcg caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct
    19561 gacgcaaatt ttgcacaaaa aataggcttt agtggtttgt ttttgttcaa aatcatgcta
    19621 aatccgtgat cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat
    19681 ccacggacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg
    19741 gttcaaatcc ggcccccgca accaatcaaa tttgatgaag taaaagcagt acggtgacgc
    19801 ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc
    19861 ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct gatctcccat
    19921 aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtt gccccatcgg
    19981 caaaatacgc tttaatcccc gcaagaatag actccgcaac ttcctgctgg aaagtcgccg
    20041 ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc agaatggagg
    20101 gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt ttatgcagct
    20161 tgttgatttt tccgagctta ttcagtaccg ctttaccaaa cttaaggctg tcagcaatag
    20221 tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg cttttgctca
    20281 cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc gcagtactgg
    20341 ttgcgccttt ggttgagagc gcaaacaccg aggaaccgct cggctgacga ctggtaaagg
    20401 cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt gctacgcgca
    20461 cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata ttgccctctt
    20521 cctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt tcgcgcgttt
    20581 tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc ataatgacaa
    20641 tcggacgatc gcgccctgct ttacccggtt gtggaccact ttgcgctggc ggcacctgct
    20701 tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc tgcatatcct
    20761 gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg accggcgcaa
    20821 gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt acggtttgcg
    20881 ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga atttgtgccg
    20941 ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc acgcgttcag
    21001 ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc gtcacgcggg
    21061 tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca gccaggctga
    21121 cctgacttac gctcaatagc cacatagcgc ccgcgccttg cagtaaacga cgacggctga
    21181 ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc tgtgtaacaa
    21241 gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc atctaataaa
    21301 cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc tcgcataaaa
    21361 tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcaa ataatcatgc
    21421 aaaggggtgt gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat tccgccattc
    21481 ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg gcggtgaagc
    21541 cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc acagcctcgg
    21601 catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc tggctgcgca
    21661 tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa cactggaact
    21721 ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt caatgagtct
    21781 caataacacg ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt ttattattgc
    21841 ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta tccggcggat
    21901 tgatgaagac gcgctccatc gtcaactgga aagcggtgca atagtgctaa tggggccggt
    21961 cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg ccactcaact
    22021 agccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccagg gcgttactaa
    22081 tgacgacggt gatattgtct ccgaactttt ccctaacgaa gcgcaagcac gggtagaagc
    22141 ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg gcgcagtgaa
    22201 agcctgccgc agcggcgtgc gccgctgtca tttaatcagt tatcaggaag atggcgcgct
    22261 gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg aaagcgccga
    22321 gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga ttcgcccgct
    22381 ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg aaatcgacaa
    22441 attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct atccgttccc
    22501 ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc gcagttcatc
    22561 acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg gcttaagcaa
    22621 attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat ttaccccggt
    22681 ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc gtaaatccaa
    22741 agtgttgatg gcggatttag ggtaagcgat gaaaatcacc ggatgcggca ggcgtaacac
    22801 tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac taaacaacgc cgccagacca
    22861 ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc ggcatacagc
    22921 gacagacgac gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt tactaccggc
    22981 gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg cgatttatgt
    23041 accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac agacttaata
    23101 ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag cgcaataccg
    23161 atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcatcac cggtcggcct
    23221 tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat aaactgttca
    23281 attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca caaaagctgg
    23341 tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag cagatccaga
    23401 taacgtccgt aacccgcaag agcttcctca agcatcgcaa tataatcttc gccgctctgt
    23461 aaaagtcgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac tgccgtttta
    23521 tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcgctgcc gaaacgatag
    23581 cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc ggttcctgcc
    23641 ggaacgtgag tccccgtcag gcggcttagc tgcctggcac gctcggcggt aaagcccgcg
    23701 ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc cagttgatca
    23761 cgatcgccga gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag tctcgacatc
    23821 ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg cagcgggtta
    23881 ccggcatgat gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg gtgcaaagtg
    23941 ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg tcgcaaagcc
    24001 ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc cagacggata
    24061 cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gcaacttcgc cacggtggtc
    24121 gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag cgccactgcc
    24181 gctgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc cagggtttgc
    24241 gccagtagag cttcatcaac ctcaatggta tgattcacct cattgaagaa gcgcgctacc
    24301 gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag gatcatcggc
    24361 gtgggttcat ctcccctgct gaccgcttga gaagccagca agcattcttc ccaattttgt
    24421 agctcaccga tttcactgac acaggtcgcc aacagcggat tcgacgcctc gttattttcc
    24481 agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa cagtgccgcc
    24541 gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg cacatccagc
    24601 gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt cattacgcct
    24661 cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg gcgttgggtc
    24721 gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg aacagataga
    24781 taacgccgcc aaagtgaaga tcatagtcgt aatcagcaat gcgatggcgc agataacgat
    24841 gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc atcgctgctg
    24901 ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat ttatagtcga
    24961 gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt aacatgccgc
    25021 gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac tggcgaatta
    25081 gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac tccatctcca
    25141 cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg agaggtgcct
    25201 ggaggacagc cgtgatccac tcggtcaaca ccggttccca ctgcgattca aagccgccga
    25261 gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg aggtcttcaa
    25321 acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc ggtgttaacg
    25381 tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt ggcatcagat
    25441 cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca cgccagttat
    25501 cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc agttaaagca tcattaacct
    25561 gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca tcgcataacg
    25621 cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc agcaaacgcc
    25681 cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg cgcaccagcg
    25741 gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa agcaaacgca
    25801 gatcttccgc cagacgttcg acctccgcga ggtcgacgct ttctggcgca gcattaagat
    25861 ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg acgcggaaat
    25921 tggtaataaa tggcaaccag accagcggat attccagccc tttcgattta tggatcgtga
    25981 caatctgcac cagatgttta tcactttcga ggcgcatttg ttggctggag gcattactgt
    26041 ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt tccagctgcg
    26101 ttccggcttc ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc cgctcaccgc
    26161 ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga catcagcgcc cgcagcatcg
    26221 gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg actaccgcat
    26281 cccacgcatg ttcgtcatta ttcagcgttt caatatccag cgcattcagc cccatcattg
    26341 acgttgccag cgcactgcgc agggtgttct cacgttcggg cgtcatcacc gcctgcaaca
    26401 accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc gaaaggtaaa
    26461 cggaagggat ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc tggcggctgc
    26521 gcaccagcac gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg ttcatcagca
    26581 acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca catacctgcg
    26641 ccatggtact ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc cacattttca
    26701 tcgcaggctg cgtttcacct ttaaatacaa aacgtaacgc ctgatttttc ccggctgatt
    26761 tcactggaat aaacggtatt tcgcgaaaca tgaacgtgtc atcagtctgg ctgaaaagct
    26821 tgttcacact gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa gtgtagtggg
    26881 catgaacttc actacgcgcc ttcatataag tgaagatatc cgcaccccgg aatgcatata
    26941 tggcctgctt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg tgccagatac
    27001 ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc attgccaccg
    27061 ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct ttcgctacgc agcgcggaat
    27121 cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt tcacgcgcta
    27181 ctgtttcgcg gatctcagcc aatgcgcggg tgatcaacag atcgcggatc gacaatggtt
    27241 ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc cccccggcct
    27301 tcgtgcgatc ttctaagaaa cgctgggaga atttttccag cgactccggc aactgataac
    27361 tgtttgtctc ttcttctgcc caggcgctga tcttttcgat ccatttagcc tgattgctac
    27421 ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt tcacccactg
    27481 cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg tggcgggaag
    27541 ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg cggttcgcct tgcagataac
    27601 gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg acctgggcta
    27661 tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc tggtagcgta
    27721 gcagagactc atcttcaatc agctgctgct caaacagcat gccggattca aaggcattca
    27781 ggttgagcat gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca tccatctgcc
    27841 gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct tccagcaggc
    27901 gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac tcgtggatat
    27961 tgctacggat acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg accaccagca
    28021 gttcttcaac gatcagcggg cggggaaagg cggcggaacc gcctagtcca agtaacaggc
    28081 gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct tcaatcaggc
    28141 gctcgccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca ctcattcttt
    28201 tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac tttccagcct
    28261 tcaggatgca catattcggc tttcccgttc tggctgccgg aaatctgcga cagaatagcc
    28321 atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg cggcgtcagc
    28381 agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc gcgatcgaaa
    28441 tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag catctgggta
    28501 attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa ttttgcctct
    28561 gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt atcattgctt
    28621 tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc aaacacggca
    28681 tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac gatctgcccc
    28741 aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac aaataccgct
    28801 gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg ttttttatcg
    28861 accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg tttttgcaca
    28921 tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat aaactctggt
    28981 cgagcccctg attttaaggc gtcgcgatag gccagcacct ctttcaacgt aatggagggc
    29041 aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat ctgcgcgggc
    29101 ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg gttataccag
    29161 gacttcgcct gctcaagctg ctcttccgta gcggtatagc taaagtaccc ctcgagcaat
    29221 gcctggaaca gctgcggcag acgctgggtg taaccattag cattaatcat aaggccgttg
    29281 ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa ctgatcaagc
    29341 gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc gctgtccatg
    29401 gctttcggat tacgcaaaat caggctgaca tcagctttgg gctcgctggc aaaataacgg
    29461 cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg atggtcgtat
    29521 ttcttctctg acttaatcag cgagaaatca tcaggaatat aagggttaag ctctggcaaa
    29581 gagagcgcaa tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc gctgatttta
    29641 tcgacctgat acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc tttcgggctg
    29701 atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt tactgcttta
    29761 gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaacagg aacgcgaatc
    29821 atggtatctg ccagccattc gacgtaatcc atatcacggg tgatcgacgg ataacggaag
    29881 tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcaatgcc tttttcacgt
    29941 aacagattga gatagctaaa aattgccgcc acaacctgat cgcgattagc caggccttta
    30001 tcagttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat aggatcggag
    30061 ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcagaaag tgtacctggg
    30121 ctgcgattgc caatcagata ggtaatcaat tcatcggttt tactacggaa cttcgctgag
    30181 ttgttatcga tgcgaaactc aacgcgcaac actttacgcg gcagcgcagg gacgtaatga
    30241 atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg ttttttgctc
    30301 tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc cggcagcggt
    30361 ttattactgt aaataaccgc cttcatcaaa ttggcggaat agtacttctc gtggaaatct
    30421 ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta aagtttcgag gttaccacca
    30481 gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg tgccatgcgc
    30541 atcccgtcac gcgtacgcgc catggttaat tcagcgttca ctgcattacg ctcacgttcg
    30601 gcgtatttct tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc taccgcaccg
    30661 ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc agtgctggca
    30721 ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg cgggtacttt
    30781 ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta cgcctcggga
    30841 tcttccagcg acccaacggg cactaccagc gccgagagcg atttaactgc ctgcggatca
    30901 gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg gcggttatct
    30961 ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc ctgacttaat
    31021 ggtgcccaga gggcaacaaa caacaataat gctttgaacc aggtgctgcg gggcattcac
    31081 ggacctcata atcaacttta ttttctggac agattcaaca ggttaacgca aatcatctgc
    31141 cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc agtccgtgac
    31201 tggtgcgcat cataaagtaa gcagatagat tgcgcaattt ttatacaaca ctcatgactg
    31261 attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg cctccattgt
    31321 ctctggtgtt aattgccgcc agagccgttg ataccagata tcatcacctt cgccacgcac
    31381 catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca acgtggaatc
    31441 gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg cgccgccact
    31501 ttcaggtaac accagcaatg gcgcggacat tccttcacga tacccctcaa tcagttgtga
    31561 gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcac cgtctttgcg
    31621 tagaaaaagg cgactttcgc cattaccacc gctggcacag tagacaaggt gttccagcca
    31681 aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca ggccatccgg
    31741 ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat caatttccat
    31801 actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca tctcctggca
    31861 ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg ccgcccggaa
    31921 gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata actgctgatt
    31981 gatttgataa cggctaagtc cttccagaat aaatggctcg gtgtctggga tttcgctgtc
    32041 ttcagtatgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg gatgtgccca
    32101 gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca gcggctgaac
    32161 aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac gagcatagct
    32221 ttgtcgttcg cctggctggt agttttgcgg atcaaacggc atccgggtat ggaggcaagt
    32281 aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat cgcccggtag
    32341 ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg ggaaacgttc
    32401 actgttatcc tgaatggaac gcccgatata gctgatatag agtttttgct gcgcggaaat
    32461 taacgcttcc aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc cacgcttcgg
    32521 tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa cgccgtcgtt
    32581 cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca gagtacaaat
    32641 gttaaccggt ccggcgagaa aacgctggct gatacgttct tgatccagac gctgtgccag
    32701 ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta aaccttcggc
    32761 gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt ccgcatccgg
    32821 caggaagaag gcgttgagca tatcgcgaca aaccggcaac cactcttcta gcggacgctc
    32881 ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agtgaagcca gatgccccac
    32941 cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc actcgccctg
    33001 cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc aggtgtgttg
    33061 tccggtggcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc aacgaatgcc
    33121 ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt caaaccgcgc
    33181 cgccagcacc ggcacatcca gcagcgccag cacatcctct gacacaaagc gactgtcagg
    33241 cagtgataac aggctgataa acgcttccag taccggatgt gactgccgcg cacgacggtc
    33301 ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacactg cctgaataaa
    33361 cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag taagtgtcgg
    33421 ggcttcctcc agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac gctgcgggct
    33481 atggcaaacg tggaatgtga tactgctatc cagtggatca agcgggcgtt tgttatcgct
    33541 acgggaaaac tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt ccagaatgtc
    33601 agactgaatg ttatgcagca ggttatctgg cgtcacatcg acaaaagcgt ccagctcctg
    33661 gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc cccatgaggc
    33721 cagcagcgag ttgccgacat cctgttcacc atcgctgtta aagagctgtc cggcattttc
    33781 gctatcgcga aatagcggca attcgcgatc ttcaaaactg tgtcgacgct gacgagtcag
    33841 cagtttcgcc agataagcag gatctttaat atcgccccag taataacggc aggggttggt
    33901 aaacaggaga tggatttcaa tatgtttacc cagcgcctgg agcgcctgga gataaacagg
    33961 cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg ggcaggtcgt
    34021 cgcggactcc agcgtttcga taaagcgctg atagagattc gcgcggtgcc agcgcggttg
    34081 tccgagttca tgggtatatt ccaccagcgc cttccacaac ggggcttgcc aggcctgtgc
    34141 ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt ccggacgata
    34201 gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca gttttcgctt
    34261 gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct ccagcaattg
    34321 cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc tctctttggg
    34381 gatttccggt aacacccgga cgaacatatc ccagataaag ctcgctggca gcggaaaatc
    34441 aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact gcgccatacc
    34501 ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt cgcgttcgac
    34561 aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga cccttaacat
    34621 agcggctcct gactactgac aattcgggca atgcaggcgg gtcatctcgc cttctctgcc
    34681 cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg tctgcattcg
    34741 gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct gccaaccatg
    34801 ccgccagagc ttttggtact ggtttctgct ggcgagactg ttcattgatg ttcgctggat
    34861 acccgataac gcagtgacaa tcatcaccat cagcaccatc agcaccatca gcaccatcag
    34921 caccatcgca accattacct ccggcaggct aaagccttgt tgattcttca gggaagctga
    34981 cataatgccc cctctttcag cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatt
    35041 tttccatcaa tgacttcgcc tgttcgccac aacgaaacgc cttcataacc ggcaatcaat
    35101 aaggcttcat tatctgccag taaacgcaaa caaacctggg catcggttcc agcgtactgc
    35161 gagcactgaa ctgctggctg cgtctgccag gagtgcattt ttccccacgc cagcgccgac
    35221 tgaacgatgg cctggcggcg caatgactga ctttccatgc tcacgcgaga ggcaaaactg
    35281 cgatcctgct gactcattcc ttgcaatagc aagctaccca aaaccagcag catcaggacc
    35341 agagccagtg acgaaacacc tttttcgcgg ttcacaggtt gaatcctgtc acgctatagc
    35401 tggcatccac cacggtttgc ggttcagact tactggcagc acgcatatta accgtcaaca
    35461 ccggcgagaa gccgctgaca tcctgacgta cgacctgaaa agtgtcgata atgatggcat
    35521 ccggattagt gactttatcc cagcccttac cttcacagga tgtcgcaccg cgtagcgttt
    35581 ccagcacatg ctccttcaga cgaaatccaa tctggtcgga ctcttttacc ggttcgcgat
    35641 cccagatacc gttactgttc gcatcccact gcacaatgat acagtcaccc tgtccgacaa
    35701 tttccagccc ttcgccggta cagctgccat gacaataacc cgccctctgg agatgcttcg
    35761 cgacggtaaa tacccgcagc cagatttcat cttccagtgc cagcttacga gtgctcgtta
    35821 aactttcacg ctgtaacgca ggcagaaagc gtgcagcccc cagcaacaat acgctactga
    35881 tcgccatagc aatcaacact tccagcagag aaaaaccttg ctcttttaca ggcatccttc
    35941 tgtttctcct tgctgataaa gccggagtct tccccacggc gaaaccacca gccaccactc
    36001 gcccgttgag tttttgaagc gaatatgccc ggcccatgcg gtattgcgca ggccaaagaa
    36061 agcaagcgaa ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca caaagaccaa
    36121 tggtgaactg ccatgacagg tattggcccc agcagcggaa ctcacaaggc accataacgt
    36181 cccctccctg ataacgctga tactgtggtc gcggttatgc cagttggcat cttcacgtaa
    36241 atagagcaaa tagtcccgcg cctagctggc ggtttgccaa agccgttgcg actgctgcca
    36301 gtattgccag ccatagagtc cacttgcgct tagcatgacc aaaatcagca tcgcgaccag
    36361 cgtttcaatc agcgtataac cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa
    36421 aaaaagacga gggccagttt ctatttcttc ggcgcatctt ccggactatt tacgccgttg
    36481 caggacgttg caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg taaaaaaacc
    36541 gacgcacact ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt
    36601 aacgcccgga tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt agtcgaagat
    36661 ggattcgggt ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg
    36721 cagatgagtt tgatccatat ggttgctgta caggtgcgta tcgccaccgg tccagacaaa
    36781 atcacccact tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc
    36841 aatgttgaac ggcaggccga ggaagacgtc acaggagcgc tgataaagct ggcaagagag
    36901 tttgccgtct gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc
    36961 cagttcgcct acgttccacg ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag
    37021 ctggttcagt accgtagtaa tctggtcaat atgacgacca tctggcgttg gccaggcgcg
    37081 ccactgttta ccatacactg gcccgaggtc gccattttca tcggcccatt cgtcccagat
    37141 ggtgacattg ttttcgtgta gataagcaat gttagtatcg ccctgcagga accacagcag
    37201 ttcgtggatg atggaacgca ggtggcaacg tttggttgtc accagcggga atccatcctg
    37261 caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt
    37321 tttctgtgtg ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc
    37381 tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc
    37441 gacaatcatc gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccggt
    37501 aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtagccaat
    37561 caggaacaag cctgagacag ctcccattgg gcgcggttta cgaatataca ggttgaggat
    37621 aataaacagc accacacctt ccagcagcag ttcgtaaagc tgtgatgggt ggcgcggcag
    37681 cacaccgtaa gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc
    37741 ttctgtacgg gagcctggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca
    37801 caattcaccg ttaataaagt tgcccagacg cccggcacca agaccaaacg gaatgagtgg
    37861 tgcgataaaa tcagagacct ggaagaagga acgtttagta cggcgggcga agataatcat
    37921 caccacgata acgccaatca ggccgccgtg gaaagacatg ccgccgtccc agacacggaa
    37981 cagatacagc ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg
    38041 tcccccgagg aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt
    38101 ccagccactg cccggacgat tcgcccgacg tgttgccagc cacattgcaa aaatgaaccc
    38161 caccagatac atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg
    38221 atcaaactcc ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt
    38281 tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc
    38341 gaataatagc acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga
    38401 tccgcgctat aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc
    38461 aacctgatga cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc
    38521 tgcttcggca aaatcaatgc gccgcagcag gtattttacc cgcgctacag aacgtccgtt
    38581 catagacaga tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc
    38641 catttcaccg cacaaacgaa gatcgatacc atgtatttcc gcttcccggg cgatcatcgc
    38701 cagagctcgt aacattgcag gatgaagact gtcgtaaatg ttcgccaccc gggtattgtt
    38761 gcgatcaacg gccagaatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac
    38821 cctctttgcc agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat
    38881 acgtggtttg ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat
    38941 caggcggcgt gcttcgtcaa cttcatcgag gctggtgacc atcggcaaca gaatattcag
    39001 gttgcccgta gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg
    39061 ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctgatcgg
    39121 catgtaaggc agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc
    39181 attgaacatt tgcagcatcc cctgatactg cgccacctgt tcttcttccg acgggaaacc
    39241 actttgcagc atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacagctgcc
    39301 cagtttttct tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc
    39361 gctttttaac tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc
    39421 ttcactaatt agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc
    39481 accgcgatag ccatcaacga tcagcgtcct acgatgcagc accgaaggct gaatatccgc
    39541 gcccatcacg gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctgc
    39601 gccatctcgc acgacaacac cgactaagcg atcctggggc agctcagcaa gagttgtcgc
    39661 tgacagttca tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc
    39721 gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt aaatcaccag cccgctcttt
    39781 gagatagtta tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac
    39841 cgcccactct gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt
    39901 gtccgaaagc aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgctccggc
    39961 ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag
    40021 tcgttcgcgt tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg
    40081 taacgtggca tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg
    40141 gattcgcgtc tggcgatatt gcccaaacag ggcagtcaac tgcgactgag aaagaatagc
    40201 tgccatctgg gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa
    40261 ctctcgttgc tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc
    40321 taaaaacgcg cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt
    40381 ttgcgcatct gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc
    40441 atcaaacgcg agcgttacag tgcgaccgcg tggttttttc agcccccggg tcgccatcag
    40501 gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc
    40561 aagacagatg tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac
    40621 cttttcgact atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg
    40681 tcgataagca gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact
    40741 cgcgaactct ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac
    40801 cggataccag taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat
    40861 attgatttct gcatcgccgc ttaccagctg caagagaaac catttttgtt tttggccgat
    40921 acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt
    40981 acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc
    41041 acggtacatc gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca
    41101 ggagtgctga ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat
    41161 acctacgttt gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt
    41221 atatacgaat gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta
    41281 cacactaata acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg
    41341 tggatagccg tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac
    41401 tacgggaaaa cccggagcat tctgaataag cccgatatag acattttaaa atattcatta
    41461 ttagtcaatg cattacatgt aatttgagta atgaaaaaga gtaattcgtg acccaggtca
    41521 cacctctcat ttacgggttg gcgaaagatc aaccaatgcc gtgtttatcc acagaatgtg
    41581 ccactaagtt aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa
    41641 atgaaaacag tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt
    41701 aagatcctgt ttattttcag tgaccagatt tggaaaacct attgcagtgt tgcgcaactc
    41761 gattaccggc agcctaaaaa gcaatataaa tcagagaatt gaacaacaca tgtggaaaaa
    41821 gttacactgc gaatattcgg cacataattg ctgtttgttt tttaaccaag gtatcatgac
    41881 atgtcccaac ctcgcccact gctctctcct cccgaaactg aagaacagtt gttagcgcaa
    41941 gcacagcaac tttctggtta tacattggga gaactggcgg cacttgacgg gctggttacg
    42001 ccagagaatt taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt
    42061 gccagcgcag ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga acttaaaact
    42121 atccctgtgg atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgcccggtta
    42181 acgggtaata gcggggtgac ctgggaaacc agccatgtgc gccacaagct caaacgcgta
    42241 ctgtggatac cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg cgtaggatca
    42301 ccgttgctgt ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga ctgggaagaa
    42361 ttaatggata tgattgttct cggtcaggtt gagcggatca ccgctcgtca cggggaatat
    42421 ttacagataa gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat tggtgcccgg
    42481 ggcgaacgga ttctgacgct gccacgcggc ttttatttga agaagaattt caccagtgca
    42541 ctactggccc gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca
    42601 tataattacc gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg
    42661 atcctaacgc ttggcttgcg ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg
    42721 acaatattat tttcctttct ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg
    42781 cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatcaatct
    42841 cttgggttac gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa atttctgccc
    42901 gtgatttgat tctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc
    42961 acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg
    43021 ctatcgtgca gattatgctg ctggatatta tcttcagcct cgactcggtg attaccgctg
    43081 tgggtctgtc agatcacctg tttattatga tggcagccgt ggtgattgcc gtaggcgtga
    43141 tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg gtaaaaatgc
    43201 tggcgctctc tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc
    43261 acgtaccgaa aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca
    43321 acttgattcg caacaaaaag aatccgctct gatactccgt acgctctcct gcgggagggc
    43381 gtactcactc cttttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc
    43441 ggtttataaa cagactatta tcataggtag gccaaatgag gataaccgga tgaaaaaatg
    43501 ggcagtaata atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta
    43561 cgtcatggcg accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga
    43621 tgataccggg ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga
    43681 tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct ggccttaaga
    43741 tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat
    43801 aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg
    43861 ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa
    43921 ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta
    43981 cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg gctggcgaaa
    44041 catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat
    44101 gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat
    44161 gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca ctggccgcag
    44221 cgcccgacca actgcttcgg caaactcggt tatagctgga cggattctgc gcctgcggtt
    44281 tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa aattcgttat
    44341 atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa
    44401 cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa ccgcagtttt
    44461 gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc
    44521 ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt
    44581 aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg
    44641 gcgtatgttg atatcgccag acgtcatggc cttgatcctg ctcagatggc gctcgcgttt
    44701 gtacgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggatcagctg
    44761 aaaactaaca tcgaaagttt gcatctggag ttaagcgaag acgtattagc tgaaattgaa
    44821 gcggtacatc aggtttatac ttatcctgca ccataaagat taacgccggt taaaaccggc
    44881 gttaaatatt aatgacggcg ctgccagatc cacagcgccg ttattgccag cgcaaatagc
    44941 gcaccgaagc caatgccaat gggcacgacc gggatgccta ccattaccgc cagcgagtaa
    45001 atgcccagca tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc aatcgcattc
    45061 cccgccccga cgcttttttt accccgctcc tgtagcaacg cattgagcgg aacgacaaaa
    45121 aagcccccca gcacgccaat cagcatcaac aaggcatagg ctggcagcag ctcgtgttgc
    45181 agggagaaaa tcggtaccac cacgccaatc aaaatcccgg ctggcataca gcgtgacacg
    45241 gtttccagcg taaccagctt cgctgccgca cctgcgccaa ccacgatacc aatcgctacc
    45301 atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac cggtacccac
    45361 agcaccaaca ggaaacgcag cgtgacaccc gctccccaga ataaactggt gcccaccagc
    45421 gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg cattcaggaa actgcgggtc
    45481 atgttgatga gattccagga ctgccccgga cgcgccgcca gtttgggaat gtagatattg
    45541 gcaacgaccg caccaccgta ggccagtgcg catgcggcca gagcgaccag gacatgccag
    45601 tcagccagca caccaccggc tacggaaccg agtaaaatcg ccgctatggc agaagcttcc
    45661 attaaaccgt tagcttttac taacttacta cccgtggtta attcgccgag aataccgtat
    45721 ttcgccggtg aataggctgc agcaccaaca cccaccagcg tatagccgag aaacggattg
    45781 ataccaaagc agatactggc tgcgcccagc agcttcaggc cgttggcaaa catcatcacc
    45841 cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgccaaaag aatgtaagca
    45901 cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg cgctttcagt
    45961 aacgccagag tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa ctgcgccacg
    46021 ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga ctcactcatc
    46081 gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt tgccgctgcc
    46141 aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct ccggcacgcc
    46201 gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg tggtgaaaag
    46261 caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga ctttatctgg
    46321 cgaaacacca agtgccagtt gttccaccat ttccagcgac accatttcgc ctgcaatttt
    46381 ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa aacgcacaat
    46441 atcgccagtg tcataccagc cgcgctccat ttcgccgcga acattctcgg cggtgggcac
    46501 ttccagtaca cctggcttct ccacccgcag atagccgttc attatgttcg gccctttcag
    46561 ttgcaggcgt ccgccctctt cgataccagg gaccgacaac aggcgtgcat ccattcctgg
    46621 tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag aaacgacagg
    46681 cgcgcattcg gtcacaccgt agccttcaag gatgcgcagg ccaaatttat cctgccaaag
    46741 ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta gacgatagaa
    46801 gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac cgaacaacac
    46861 ggtgcaactg cggtcataca ccaagtccgg cacaatgcgg taatgcagcg ggcttggata
    46921 aaggaacact tctgcacctg taagcagtgg cgtaaacagg cctaccgtca gcccaaagga
    46981 gtgaaacagc ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg caatcgtttt
    47041 aatctgctcg acgttcgcca gaatgctttt atggctatgg acgacgcctt ttggatggcc
    47101 ttcagaaccg gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt ttacctgcgc
    47161 caggcgcggc atcagcaaat gagcgaagat ccatactttg tcggcagtgg tgacatctgc
    47221 ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca gatgccagag
    47281 tttgccttta tcgagaaact ggcgggaagt gaagatggtt ttgatttcag ccgccgtaat
    47341 agcactggtc agccctttta ccccggcagt gtagttcatc attgcgggaa tgcggcgacg
    47401 cgcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca ttaagccgat
    47461 gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg ttttcgtaag
    47521 caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct tcccggctcc
    47581 gaagcggtac attgcactca gtaaagattc gtacagagtt tcacgcgggc gcaccgccat
    47641 acgcgcttcc atcattattt gatgcagcat ttcgccagcg attttgcgac ggtcacgggc
    47701 acgcggcgca tccggcatcg ccacctgcgt tggaggcaaa atatgcagag taatttgcgg
    47761 gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt ccgccccttc
    47821 aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg cgccatcgta
    47881 gattttcatc agcgagcctg tagtggtgat gcgtccttca gggaaaatca ccactggtcg
    47941 gccctgttcc accagacgta ccagatgttt aatagccata ggttgcgtcg ggtcgagagg
    48001 aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta ttgaggtgta
    48061 aacggcaaac actggacgca caggtaaaaa cagtccaagc aaaatgccat caataaaaga
    48121 gacgtgatta ggcgtaatta gtacgcgctc gcccttcagt gcctgggtgt cacccgtaac
    48181 gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca ttccaactcc
    48241 ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa gaacgcgagc
    48301 gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg caagagacag
    48361 ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatct tgattgtggt
    48421 ctgcacgacg actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa tgcaacgagg
    48481 tgtgtaaatt gtcggttact gttacagatt gatgaccggc aaaaaaaaac ctgcgcatct
    48541 gcgcaggctg gtgtaattca tgtgctcaac acgaagttga cttcacctat caatacctct
    48601 gggatcacca ctttagcaac ctgaagccaa acgccaccag cggtcaatcg caacagcgtt
    48661 tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt cttacttatt tgcgtttggc
    48721 tcacattccc acgatgaaaa cacgccaccc cttgaaccaa cgggcgtttt ccgtaacact
    48781 gaaacaatgt aagcgtttac ccactaaggt attttcatgg cgaccataaa ggatgtagcc
    48841 cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc acccaaagcc
    48901 agcgaagctt cccggctggc tgtgcatagt gcaatggagt ctcttagcta tcacccgaac
    48961 gccaacgccc gtgcgctggc gcagcagacc actgaaacgg tcggtctgat cgttggtgat
    49021 gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc ttatcacacc
    49081 ggtaatttct tattgattgg caacggttac cacaacgaac aaaaagagcg tcaggctatt
    49141 gagcaactga tccgccatcg ctgtgctgcg ttggtcgtcc atgccaaaat gatcccagat
    49201 gccgatttag cctcattaat gaaacaaatg cccggtatgg tgctgatcaa ccgtatcctg
    49261 cctggctttg aaaaccgttg tattgctctt gacgatcgtt acggtgcctg gctggcaacg
    49321 cgtcatttaa ttcagcaagg tcatacccgc attggttatc tgtgctctaa ccactctatt
    49381 tctgacgccg aagatcgtct gcaagggtat tacgatgccc ttgctgaaag tggtattccg
    49441 gccaatgatc ggctggtgac atttggcgaa ccagacgaaa gcggcggcga acaggcaatg
    49501 accgagcttt tgggacgagg aagaaatttc actgcggtag cctgttataa cgattcaatg
    49561 gcggcgggcg cgatgggcgt tctcaatgat aatggtattg atgtaccggg tgagatttcg
    49621 ttaattggct ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct gaccaccgtg
    49681 cgttacccaa tcgtaacgat ggcgacccag gctgccgaac tggctttggc actggcggat
    49741 aatcgccctc tcccggaaat tactaatgta tttagtccga cgctggtacg tcgtcattca
    49801 gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca gcgactaacc gcagttaaag
    49861 caattccagc gccagtaatt cttcgatggt ctggcgacgg cgaatcaacc gcgcctgacc
    49921 attatcaaac agaacttctg gtaacagcgg acggctattg tagttggatg acattgatgc
    49981 gccatatgcc cctgtatcat gcagtaccag ataatcacct gccttcactt ccggcaaggc
    50041 gcgggtttca acatttcccc cttcctgctg ggtaaagaca tcgcccgatt cacataacgg
    50101 cccggcgacg acggtttcca ccgttggcgc gtgttccaga gaacgaccat cagctgccag
    50161 ggcactgata tggtggtaac taccgtacat tgccgggcgc atcagatcgt tgaacccggc
    50221 atcaaccagc acaaagtggc ggctacccat ttgtttgacg ctccgcacct gagtaattaa
    50281 tacgccagac tgcgctacca ggaagcgacc cggttcaatt tccagtttca cagggtggcc
    50341 caaatggcga gcgatttgct cacgcgcggc attccacaga ccataataat gttcggtatc
    50401 aaccgcctct tcaccctgtt gataaggaat agaaagccca ccgcccgcag aaatagcctg
    50461 taaatcctga ccgaattcga taacctgacg caccatagca ccacacacct gctccagatg
    50521 ggcataatca acgccagaac caatgtgcat gtgaatgccg actagctgca gatgatgacg
    50581 ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct tgctgttttc
    50641 gccaccggta ttggtttttt ggctatgtcc gtgaccaaac cccggattaa cgcgcagcca
    50701 tacccgatgc cctggcgaaa cctggcccag ttggtcgagc gtatcaacag aacccgcatt
    50761 caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa catctgccgt
    50821 aaaaacaata tcatcggggt gcgtttgcgg attgtaaccc gccgccaacg cacgctctat
    50881 ttcgcctaac gagacggaat ccactttcac gccctgctca cgcattaagc gcaaaatatg
    50941 aatattggaa caggctttct gtgcaaagcg caccacttca aactgtttca gcgctgcaat
    51001 ctgccgacga ataatttgcg catcgtagac ccacaccggg cagccaaatt cagcgggcaa
    51061 acgcagcaga ttttcggcgg tgagatcggt atcggtgctg aacagtgaat gtggcataac
    51121 aaactccaga tgagtgcttt tttatgatta cgccacatca taaaaagaat aaaaaatatc
    51181 gatttatgtc gagtctatgc aaaaatgata tgaattaccg gattgcgaga gagcgctaat
    51241 ggccgccgtt aacttacgtc atattgaaat ttttcatgcg gtaatgaccg ccggaagcct
    51301 gactgaggca gcacacctgc tacacacctc acagccaacc gtcagccgcg aactggcgcg
    51361 ctttgagaag gtgatcgggc tgaaattgtt tgagcgcgta cgtggacgat tacatcctac
    51421 cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac tggatcgcat
    51481 tgtcagcgcc gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta ttgcctgcct
    51541 gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg cacgttatcc
    51601 cgatgtcagc ttaaatatcg tgccccagga atcaccgcta cttgaagagt ggctctcggc
    51661 ccagcgtcat gatttaggac tcactgagac actccatacg cctacgggaa cagaacgtac
    51721 cgaattactc tctttagatg aagtgtgtgt gttacctccg ggtcatccgc tggcggtaaa
    51781 aaaggtatta acgccggatg attttcaggg tgagaattac atcagccttt cccgtactga
    51841 cagctatcgc cagttgctgg atcagctatt tactgaacat caggttaaac gacgcatgat
    51901 cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta cgggcggggg taggtatttc
    51961 ggtggttaac ccgctcaccg cactagatta tgcggcaagc ggtttagtgg tgcggcggtt
    52021 cagtattgcg gttccgttca ccgtcagcct gatccgcccc ctgcaccgcc cgtcatcagc
    52081 gctggttcag gcgtttagtg ggcatttaca agcggggtta ccgaaactgg tcacttctct
    52141 tgacgctatt ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc ggcatggatc
    52201 gccgcggtat caaacacagg caaaacactg cgctcttctg gcaccagtaa accaatttct
    52261 gtgcagccaa aaatgacgcc ctgtgcgccc tgttctgcaa ggcgagcaat cacttgcgca
    52321 taataagcgc gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa aataatctga
    52381 ttaattttcg cccgttcatc cgcttcagga ataagacaat tgatggaaaa ttgttccgtc
    52441 agccgcccgc gataaaaatc ctgttccatg gtgtaacgcg tacccagcag cgccacacga
    52501 gtcattccgg ccccggtaat tgcacgtccg gtggcatccg caatgtgtaa gaaaggcaga
    52561 gtgcaacgtg actcaatggc atccgccact ttatgcatcg tattggtaca taacacaata
    52621 ccttctgcgc ccgcccgctg taagccaagc gccgcctcag ccagaatgtc cccggtttta
    52681 tcccattccc cgcgacgctg gcactcttct atttcatgaa aatcgacgct atgtagcagc
    52741 atttgcgcag agtgaagccc accaagccgc tgtttaatgc cttcatttat cagacgatag
    52801 taaggaatgg tggattccca gctcattcct cccagcaaac caattgtttt cattcctctc
    52861 tcctgatgtg tgttaccgca acaaggctga ataaggaacg agacaaacgc ctcaacggcc
    52921 aggtgcccaa tctctattaa cgaaaaaagg gccggatgta cagcacatcc ggcccgtgaa
    52981 atcagacgcc gatatttctc aacttctcgc ctgccatcag tttgcgttcg atatgttcca
    53041 gcgtgacatt tttggtttcc ggaatgagcc agaaagtaat acccacaaac gcaatgttca
    53101 gcgcagtgta gagccagaac gtaccggcag cgccaatgct atcaagcagt gtcaggaagg
    53161 ccgcgccgat aatcatattc gacacccagt ttgtcgtcgt cgaacaggta ataccgaaat
    53221 cgcggcattt cagcggctga atttcagagc acaggatcca caccactggc gcggcgctca
    53281 tcgcataacc ggcaatacac atcatcgtca tgccaacaga gagccaggac aagccactgg
    53341 aagccgtacc gttatcaaac tgcatcaggc aatagcccag caccagagtg cctaacgcca
    53401 tcacgctgaa accaattttc agagccggtt tacgacctgc tttatctacc gtaaagaccg
    53461 caatgaaggt cgcgaacata aaggtcagtc ccacgaccag agtcgcaatc atctgttgtt
    53521 ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc gtagtacatg atgatgttca
    53581 taccggtaaa ctgctgcatc gcctgcaaca acataccgag aaacacagca cgacggacgt
    53641 tacggttgat cttaaacagt gcccaaccgc cctgttttaa cttcaggctt tcacgaattt
    53701 cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag catacgcaac acttcttccg
    53761 cctcaatatg acgccccttt tccgccagcc agcgcgggct atttggcagg aagaccacca
    53821 gaataatcag cagaactgct ggtaaagcaa gaacccccaa cattgcgcgc cagttaccgc
    53881 tataactgaa cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg accatcaact
    53941 ggtacatact gatcatctta ccgcgaacgt tttcacttgc catttcagaa agatacagag
    54001 gagcggtgta agacgcgatc ccgacagcaa tgcccagcac cacacgagcg gcgattaaca
    54061 tctctacgct ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg atggcccccg
    54121 ccatcaggct gtatttacgc cccaggcgga acgacagcca accattaaac agcgcaccaa
    54181 ttgctgcgcc gagcatcatg ctactaacca cccattcctg caaacgactg gtcagcacaa
    54241 agtgatcggt aatgaacggc aacgctccgg cgattacgcc gatatcaaga ccaaataaca
    54301 atcctgcgac cgcagcagct accgaaacaa acatattcat acgccgcgta tcacgcaaag
    54361 aacgtggcgt taaagcagat tccgtattga tagtaaccat tttttcctgc cagcagagag
    54421 taagacattg tgaaaaaata cgtgaacaat tcacacaggt gtcaggtcgg aaacagcata
    54481 aatatggatt aaattgctgc gacatgccgt tatgtgatgg atattccaat tttaaaatta
    54541 agttgaatta ttgagattat tattaacctc ctaattttaa agcagataaa attcataaag
    54601 ttcattaatt gataattgat atggattatt tcataaccat gatatggatt atgatgatct
    54661 agagatataa aaaaccctgc catgcggcag ggtcataaaa gtaagaggaa tgaattaacg
    54721 cgccagccaa ccgccatcca cagcaatggt ataaccattt acataatctg aagcgctgga
    54781 ggcaagaaac actaccggcc ccatcaggtc actcggcagt ccccaacggc cagctggaat
    54841 gcgatcgaga atttccgcgc tacgttgttc atctgcccgc agttgttggg tattgttggt
    54901 cgccatgtaa cccggagcta tcgcattaac attaatgttg tgtttagccc attcgtttgc
    54961 catcaatcgc gtcacaccca tcacgccgct ttttgatgcg gtataagaag gcacacggat
    55021 cccgccctgg aaggagagca ttgacgcgat attgataatc ttgccgccat tgccttgcgc
    55081 gataaagtgt ttcgccgctg cctgagacat gaagaatacg ctcttgatat tcaggttcat
    55141 gacatcgtcc cagtcttttt cgctgaactc gagagcgtct tcgcggcgaa tcaatccggc
    55201 gttattcacc aggatatcaa tatgaccaaa ttcagctacc gcgcgatcca gcagtccagg
    55261 aatgccatca atctttcgca gatcggcggt caggcttaaa aaacgacgcc ccagcgccgt
    55321 gacctgctcg atggtttcag tcggttcaac gatgttaatg ccaacaatgt cacagcccgc
    55381 ttgcgccagc cccaacgcca tcccctggcc cagcccagta tcacaaccag tgacgaccgc
    55441 aactttacct tggagagaaa atgcacttaa aatcataaca acaccttatt cgtttatgcc
    55501 cacaactagc gcaaatcttt aacgcccaca tggtccatat cattaaagac ctggttttca
    55561 ccgaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg gatcgaccag
    55621 ctcggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc ttgcggctgc
    55681 cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata aacttccatc
    55741 cggcgctcgt gggtgtgaca cggcatggtg ttccacaagt tacctggagc cagctccgtc
    55801 agccccatac tcaattgaca ggtttccagt acatccggga caaaatattt gttaatcgtg
    55861 cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc cggtgtgact
    55921 tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt cgccggagtg
    55981 gcggtatcaa tactggcaaa gacaacttct tttgcacctt taccaacata cagggcgtcg
    56041 cggtgaccga tttcatagca ttgaccatcg acagtaatcg taccggcacc gccaatattg
    56101 ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt accaacttcc
    56161 ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat tcggtcaatg
    56221 tggctgtaaa ccatggtgta ctcatcggcg acaaatacct tttcaaccaa aaattcattg
    56281 cgcagccctt gggtatccag cgtttttgcg tgcgcaccgt ggatgctctg tcttacgtcc
    56341 acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga aacactgttt
    56401 cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta aaaataaaac
    56461 aatgttttat ttttaattta aggattaaaa aaagtgcagt gtcgatcacg aatatgtcat
    56521 gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc atcattattc
    56581 aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct actcgtcacg
    56641 ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca gccctttgcg
    56701 ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac cggaagcgcc
    56761 tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct catcccactc
    56821 aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct cgataagatc
    56881 gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat acaccggcgc
    56941 aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc gggccagcac
    57001 gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag ctgcgccatc
    57061 gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg aaggatttaa
    57121 acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat caacaaccaa
    57181 cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc gtccggcgtc
    57241 aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca actgacggct
    57301 gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat cattgaaggc
    57361 atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa gctgttcacc
    57421 ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc cacctgcgat
    57481 gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg ctttaagacc
    57541 ggaaccgcaa acgtcattaa tcgtgattgc agaaacgctg ttaggaagac cacctttaat
    57601 agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac caagaattac
    57661 ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca cgaccagact
    57721 accaagttcc acggcggaat gacccgctaa cgcaccacga aagcagccga taggtgtccg
    57781 taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa cagtgcaata
    57841 gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt ttatcacaaa
    57901 ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg caaccactaa
    57961 cccaccaata gatgggtaga tagggcgtta atctcccata cttaacttgg tttatggtaa
    58021 attgccctcc attttgttta atttgtagat gatacgttca gataatgtct aatatttggt
    58081 ctaaagaaga aactctgtga agtttcgcgc tctatggcac agccgttgat gcaggcacgc
    58141 tattccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt actgctctgg
    58201 tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc ctctcatcaa
    58261 aaacatcagc aggtgaaggg ataacgggcg cggtaacaca ctactatggc aagaagattg
    58321 gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg atatatgcag
    58381 tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt gatcttcgca
    58441 tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa tctcattttt ctgatgggac
    58501 gtcatgccac tattcgggta atgggatttt tggtattccc attgattgcc tatttcttat
    58561 ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacaacc caggtagagt
    58621 tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt ttcgccttta
    58681 gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat ggcgaacacg
    58741 ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc ataagtgtac
    58801 tgttctttgt ctttagctgc ctgctttcta ttccatcttc gtatattgaa gcggctaaag
    58861 aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca gcatggttgt
    58921 caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg ggtacgtact
    58981 ttggcgttat tgaaggtgcc acagaggtcg tcaaaacaac actacagcag gttggtgtaa
    59041 agaaaagtcg tgcatttaac cacgcactat caattatgtt ggtatcgctg attaccttca
    59101 ttgtttgttg cattaacccg aacgcgattt cgatgattta cgcgatcagc ggcccgctca
    59161 ttgccatgat acttttcatc atgcctacgc tgtcaacgta tctcatcccg gcgcttaaac
    59221 cctggcgttc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc gtatcggtaa
    59281 tgttctttag ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt gaaccattcc
    59341 atttttcata atattcattt gttattattc cgccctatca cttctctcgt catatcctag
    59401 aattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga ctatttaaga
    59461 tgcataactt agattcgcaa gatatcttct ggtcattcaa aaacaatttg cgatatagat
    59521 attctcatac catgagatag ttatctggac cacgttcgac catgcaagat taaatcagtg
    59581 aaacataatc atatttaata acgagaaatg catttttaaa tatcttaatc agaattatag
    59641 caaatacaga ttaatcgcaa tttttcccag caaaattaca aaatcaaggt gtattgacat
    59701 catcaatcac caatgaaatt atgcaattat atacggatag ggaggttctt aacatggggc
    59761 tttgtagtcg ttataaaagt cttacatgca atagttgctc aatgcattgc caaataatgc
    59821 cagaagagtc accgcgtttg cagtactgcg caaactcgt
//
LOCUS       DAZSNO010000007        55418 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZSNO010000007 DAZSNO010000000
VERSION     DAZSNO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55418
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.007"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHWN2_000379"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHWN2_000379"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR8930073.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHWN2_000380"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHWN2_000380"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR8930074.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHWN2_000381"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHWN2_000381"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR8930075.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="ACHWN2_000382"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="ACHWN2_000382"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR8930076.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="ACHWN2_000383"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="ACHWN2_000383"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR8930077.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="ACHWN2_000384"
     CDS             7826..9559
                     /locus_tag="ACHWN2_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR8930078.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="ACHWN2_000385"
     CDS             9708..10559
                     /locus_tag="ACHWN2_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8930079.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="ACHWN2_000386"
     CDS             complement(10546..10887)
                     /locus_tag="ACHWN2_000386"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR8930080.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="ACHWN2_000387"
     CDS             complement(10889..11767)
                     /locus_tag="ACHWN2_000387"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR8930081.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="ACHWN2_000388"
     CDS             complement(11733..14030)
                     /locus_tag="ACHWN2_000388"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR8930082.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="ACHWN2_000389"
     CDS             complement(14081..14401)
                     /locus_tag="ACHWN2_000389"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR8930083.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="ACHWN2_000390"
     CDS             complement(14416..15495)
                     /locus_tag="ACHWN2_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR8930084.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHWN2_000391"
                     /pseudo
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHWN2_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="ACHWN2_000392"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="ACHWN2_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="ACHWN2_000393"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="ACHWN2_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="ACHWN2_000394"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="ACHWN2_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHWN2_000395"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHWN2_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR8930085.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHWN2_000396"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHWN2_000396"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR8930086.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHWN2_000397"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHWN2_000397"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR8930087.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25474..25608
                     /locus_tag="ACHWN2_000398"
     CDS             25474..25608
                     /locus_tag="ACHWN2_000398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930088.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHWN2_000399"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHWN2_000399"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR8930089.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHWN2_000400"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHWN2_000400"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR8930090.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="ACHWN2_000401"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="ACHWN2_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR8930091.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="ACHWN2_000402"
     CDS             30120..30728
                     /locus_tag="ACHWN2_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR8930092.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHWN2_000403"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHWN2_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR8930093.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="ACHWN2_000404"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="ACHWN2_000404"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR8930094.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="ACHWN2_000405"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="ACHWN2_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR8930095.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHWN2_000406"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHWN2_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR8930096.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="ACHWN2_000407"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="ACHWN2_000407"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR8930097.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="ACHWN2_000408"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="ACHWN2_000408"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR8930098.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="ACHWN2_000409"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="ACHWN2_000409"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR8930099.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="ACHWN2_000410"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="ACHWN2_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR8930100.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHWN2_000411"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHWN2_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR8930101.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="ACHWN2_000412"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="ACHWN2_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="ACHWN2_000413"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="ACHWN2_000413"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR8930102.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="ACHWN2_000414"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="ACHWN2_000414"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR8930103.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="ACHWN2_000415"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="ACHWN2_000415"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR8930104.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHWN2_000416"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHWN2_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR8930105.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="ACHWN2_000417"
     CDS             complement(44843..45142)
                     /locus_tag="ACHWN2_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR8930106.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="ACHWN2_000418"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="ACHWN2_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="ACHWN2_000419"
     CDS             45895..46494
                     /locus_tag="ACHWN2_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR8930107.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKIQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHWN2_000420"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHWN2_000420"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR8930108.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHWN2_000421"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHWN2_000421"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR8930109.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHWN2_000422"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHWN2_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR8930110.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHWN2_000423"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHWN2_000423"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR8930111.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHWN2_000424"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHWN2_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR8930112.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHWN2_000425"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHWN2_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR8930113.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHWN2_000426"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHWN2_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR8930114.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHWN2_000427"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHWN2_000427"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR8930115.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHWN2_000428"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHWN2_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR8930116.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13492 a        15016 c        14165 g        12745 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcgc cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccagacgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttatttacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaatacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DAZSNO010000008        55328 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZSNO010000008 DAZSNO010000000
VERSION     DAZSNO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55328
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.008"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(105..1769)
                     /gene="glnS"
                     /locus_tag="ACHWN2_000429"
     CDS             complement(105..1769)
                     /gene="glnS"
                     /locus_tag="ACHWN2_000429"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR8930117.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1972..3918)
                     /gene="nagE"
                     /locus_tag="ACHWN2_000430"
     CDS             complement(1972..3918)
                     /gene="nagE"
                     /locus_tag="ACHWN2_000430"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR8930118.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4251..5051
                     /gene="nagB"
                     /locus_tag="ACHWN2_000431"
     CDS             4251..5051
                     /gene="nagB"
                     /locus_tag="ACHWN2_000431"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR8930119.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5111..6259
                     /gene="nagA"
                     /locus_tag="ACHWN2_000432"
     CDS             5111..6259
                     /gene="nagA"
                     /locus_tag="ACHWN2_000432"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR8930120.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6268..7488
                     /gene="nagC"
                     /locus_tag="ACHWN2_000433"
                     /gene_synonym="nagR"
     CDS             6268..7488
                     /gene="nagC"
                     /locus_tag="ACHWN2_000433"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR8930121.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7536..8288
                     /gene="nagD"
                     /locus_tag="ACHWN2_000434"
                     /pseudo
     CDS             7536..8288
                     /gene="nagD"
                     /locus_tag="ACHWN2_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            complement(8339..8437)
                     /locus_tag="ACHWN2_000435"
                     /pseudo
     CDS             complement(8339..8437)
                     /locus_tag="ACHWN2_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8685..10349
                     /gene="asnB"
                     /locus_tag="ACHWN2_000436"
     CDS             8685..10349
                     /gene="asnB"
                     /locus_tag="ACHWN2_000436"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR8930122.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10728..10804
                     /locus_tag="ACHWN2_000437"
     tRNA            10728..10804
                     /locus_tag="ACHWN2_000437"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10762..10764,aa:Met,seq:cat)
     gene            10813..10897
                     /locus_tag="ACHWN2_000438"
     tRNA            10813..10897
                     /locus_tag="ACHWN2_000438"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10847..10849,aa:Leu,seq:tag)
     gene            10921..10995
                     /locus_tag="ACHWN2_000439"
     tRNA            10921..10995
                     /locus_tag="ACHWN2_000439"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10953..10955,aa:Gln,seq:ttg)
     gene            11030..11104
                     /locus_tag="ACHWN2_000440"
     tRNA            11030..11104
                     /locus_tag="ACHWN2_000440"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11062..11064,aa:Gln,seq:ttg)
     gene            11120..11196
                     /locus_tag="ACHWN2_000441"
     tRNA            11120..11196
                     /locus_tag="ACHWN2_000441"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11154..11156,aa:Met,seq:cat)
     gene            11245..11319
                     /locus_tag="ACHWN2_000442"
     tRNA            11245..11319
                     /locus_tag="ACHWN2_000442"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11277..11279,aa:Gln,seq:ctg)
     gene            11357..11431
                     /locus_tag="ACHWN2_000443"
     tRNA            11357..11431
                     /locus_tag="ACHWN2_000443"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11389..11391,aa:Gln,seq:ctg)
     gene            complement(11585..12760)
                     /gene="ubiF"
                     /locus_tag="ACHWN2_000444"
     CDS             complement(11585..12760)
                     /gene="ubiF"
                     /locus_tag="ACHWN2_000444"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR8930123.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12906..14330
                     /gene="miaB"
                     /locus_tag="ACHWN2_000445"
     CDS             12906..14330
                     /gene="miaB"
                     /locus_tag="ACHWN2_000445"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR8930124.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDASHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14483..15523
                     /locus_tag="ACHWN2_000446"
     CDS             14483..15523
                     /locus_tag="ACHWN2_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR8930125.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15520..15987
                     /gene="ybeY"
                     /locus_tag="ACHWN2_000447"
     CDS             15520..15987
                     /gene="ybeY"
                     /locus_tag="ACHWN2_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR8930126.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16077..16955
                     /gene="corC"
                     /locus_tag="ACHWN2_000448"
     CDS             16077..16955
                     /gene="corC"
                     /locus_tag="ACHWN2_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR8930127.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16980..18518
                     /gene="lnt"
                     /locus_tag="ACHWN2_000449"
     CDS             16980..18518
                     /gene="lnt"
                     /locus_tag="ACHWN2_000449"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR8930128.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18915..19823
                     /gene="gltI"
                     /locus_tag="ACHWN2_000450"
     CDS             18915..19823
                     /gene="gltI"
                     /locus_tag="ACHWN2_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR8930129.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19993..20733
                     /gene="gltJ"
                     /locus_tag="ACHWN2_000451"
     CDS             19993..20733
                     /gene="gltJ"
                     /locus_tag="ACHWN2_000451"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR8930130.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20733..21407
                     /gene="gltK"
                     /locus_tag="ACHWN2_000452"
     CDS             20733..21407
                     /gene="gltK"
                     /locus_tag="ACHWN2_000452"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR8930131.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21407..22132
                     /gene="gltL"
                     /locus_tag="ACHWN2_000453"
     CDS             21407..22132
                     /gene="gltL"
                     /locus_tag="ACHWN2_000453"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR8930132.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22250..23185
                     /gene="rihA"
                     /locus_tag="ACHWN2_000454"
     CDS             22250..23185
                     /gene="rihA"
                     /locus_tag="ACHWN2_000454"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR8930133.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            23269..24939
                     /gene="hscC"
                     /locus_tag="ACHWN2_000455"
                     /pseudo
     CDS             23269..24939
                     /gene="hscC"
                     /locus_tag="ACHWN2_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(24999..26450)
                     /gene="djlC"
                     /locus_tag="ACHWN2_000456"
     CDS             complement(24999..26450)
                     /gene="djlC"
                     /locus_tag="ACHWN2_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR8930134.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(26447..27154)
                     /locus_tag="ACHWN2_000457"
     CDS             complement(26447..27154)
                     /locus_tag="ACHWN2_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR8930135.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            27256..27810
                     /locus_tag="ACHWN2_000458"
     CDS             27256..27810
                     /locus_tag="ACHWN2_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR8930136.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(27820..29247)
                     /locus_tag="ACHWN2_000459"
     CDS             complement(27820..29247)
                     /locus_tag="ACHWN2_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR8930137.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(29244..29951)
                     /locus_tag="ACHWN2_000460"
     CDS             complement(29244..29951)
                     /locus_tag="ACHWN2_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR8930138.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            30115..31092
                     /gene="ybeQ"
                     /locus_tag="ACHWN2_000461"
     CDS             30115..31092
                     /gene="ybeQ"
                     /locus_tag="ACHWN2_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR8930139.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(31162..31644)
                     /locus_tag="ACHWN2_000462"
     CDS             complement(31162..31644)
                     /locus_tag="ACHWN2_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR8930140.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            31879..34461
                     /gene="leuS"
                     /locus_tag="ACHWN2_000463"
     CDS             31879..34461
                     /gene="leuS"
                     /locus_tag="ACHWN2_000463"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR8930141.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            34476..35057
                     /gene="lptE"
                     /locus_tag="ACHWN2_000464"
     CDS             34476..35057
                     /gene="lptE"
                     /locus_tag="ACHWN2_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR8930142.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            35057..36088
                     /gene="holA"
                     /locus_tag="ACHWN2_000465"
     CDS             35057..36088
                     /gene="holA"
                     /locus_tag="ACHWN2_000465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR8930143.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            36090..36731
                     /gene="nadD"
                     /locus_tag="ACHWN2_000466"
     CDS             36090..36731
                     /gene="nadD"
                     /locus_tag="ACHWN2_000466"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR8930144.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            36755..37366
                     /locus_tag="ACHWN2_000467"
     CDS             36755..37366
                     /locus_tag="ACHWN2_000467"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFR8930145.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            37626..37943
                     /gene="rsfS"
                     /locus_tag="ACHWN2_000468"
     CDS             37626..37943
                     /gene="rsfS"
                     /locus_tag="ACHWN2_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR8930146.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            37947..38414
                     /gene="rlmH"
                     /locus_tag="ACHWN2_000469"
     CDS             37947..38414
                     /gene="rlmH"
                     /locus_tag="ACHWN2_000469"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR8930147.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            38445..40346
                     /gene="mrdA"
                     /locus_tag="ACHWN2_000470"
     CDS             38445..40346
                     /gene="mrdA"
                     /locus_tag="ACHWN2_000470"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR8930148.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            40349..41461
                     /gene="mrdB"
                     /locus_tag="ACHWN2_000471"
                     /gene_synonym="rodA"
     CDS             40349..41461
                     /gene="mrdB"
                     /locus_tag="ACHWN2_000471"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR8930149.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            41472..42560
                     /gene="rlpA"
                     /locus_tag="ACHWN2_000472"
     CDS             41472..42560
                     /gene="rlpA"
                     /locus_tag="ACHWN2_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR8930150.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            42699..43910
                     /gene="dacA"
                     /locus_tag="ACHWN2_000473"
     CDS             42699..43910
                     /gene="dacA"
                     /locus_tag="ACHWN2_000473"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR8930151.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            44021..44284
                     /gene="ybeD"
                     /locus_tag="ACHWN2_000474"
     CDS             44021..44284
                     /gene="ybeD"
                     /locus_tag="ACHWN2_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR8930152.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            44385..45026
                     /gene="lipB"
                     /locus_tag="ACHWN2_000475"
     CDS             44385..45026
                     /gene="lipB"
                     /locus_tag="ACHWN2_000475"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR8930153.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            45285..46238
                     /locus_tag="ACHWN2_000476"
     CDS             45285..46238
                     /locus_tag="ACHWN2_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR8930154.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            46447..47412
                     /gene="lipA"
                     /locus_tag="ACHWN2_000477"
     CDS             46447..47412
                     /gene="lipA"
                     /locus_tag="ACHWN2_000477"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR8930155.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(47513..47716)
                     /gene="tatE"
                     /locus_tag="ACHWN2_000478"
     CDS             complement(47513..47716)
                     /gene="tatE"
                     /locus_tag="ACHWN2_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR8930156.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(47845..48633)
                     /locus_tag="ACHWN2_000479"
     CDS             complement(47845..48633)
                     /locus_tag="ACHWN2_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR8930157.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            48726..49109
                     /gene="crcB"
                     /locus_tag="ACHWN2_000480"
     CDS             48726..49109
                     /gene="crcB"
                     /locus_tag="ACHWN2_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR8930158.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(49163..49372)
                     /gene="cspE"
                     /locus_tag="ACHWN2_000481"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(49163..49372)
                     /gene="cspE"
                     /locus_tag="ACHWN2_000481"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR8930159.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(49547..50107)
                     /gene="pagP"
                     /locus_tag="ACHWN2_000482"
     CDS             complement(49547..50107)
                     /gene="pagP"
                     /locus_tag="ACHWN2_000482"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR8930160.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            50696..52081
                     /gene="dcuC"
                     /locus_tag="ACHWN2_000483"
     CDS             50696..52081
                     /gene="dcuC"
                     /locus_tag="ACHWN2_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR8930161.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(52122..52463)
                     /locus_tag="ACHWN2_000484"
                     /pseudo
     CDS             complement(52122..52463)
                     /locus_tag="ACHWN2_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(52801..53310)
                     /locus_tag="ACHWN2_000485"
     CDS             complement(52801..53310)
                     /locus_tag="ACHWN2_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR8930162.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            53482..53718
                     /locus_tag="ACHWN2_000486"
     CDS             53482..53718
                     /locus_tag="ACHWN2_000486"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8930163.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            53693..55324
                     /locus_tag="ACHWN2_000487"
                     /pseudo
     CDS             53693..55324
                     /locus_tag="ACHWN2_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT        13891 a        13930 c        13841 g        13666 t
ORIGIN      
        1 gcctgataag cgaagcgcat caggcaattt agcgtttgaa atttattgtc tatttattgc
       61 cggatgcgac gtaaatgcct cacccggctt ggtagaactt aaaattactc gcctactttc
      121 gcccaggtat cacgcagccc aacggtgcgg ttaaataccg gtttttcggc cgtggaatgg
      181 cggctgtcga ggcagaagta accttcacgc tcaaactgga atgctttacc cgcaaccgca
      241 tctttcagcg acggttcagc aaagccctgt ttgatcacca gcgattccgg gttaatcacc
      301 gacaggaaat catccgcagc acctgggtta ggcacgctga acagacgatc atacaaacgg
      361 atttcaaccg gcagcgcatg tgccgcgctc acccagtgaa taacaccttt gactttacga
      421 ccatctgccg gatctttgct taaggtgtcg gcgtcataag tacagaagat ggtggtgata
      481 ttaccttcgg catctttctc cacgcgttca gccttaatga cgtaagcatt acgcagacgc
      541 acttctttac ccagcaccag acgtttgtac tgcttgttag cttcttcgcg gaaatcggcg
      601 cgatcgatcc aaatttcacc gctaaacggc acctgacggc tccccatttc cggtttgttc
      661 ggatggttcg gcatggtgac catttcgcct tcgccctgat agttttcgat aaccagtttc
      721 accggatcga taaccgccat tgcgcgcggc gcattttcgt tgagatcttc acggatgcag
      781 gattccagcg acgccatctc aatggtgttg tcctgcttgg tcacgccgat gcgtttgcag
      841 aactcacgaa tagaagccgc agtgtaacca cgacgacgca gaccggaaat ggtcggcata
      901 cgcgggtcat cccagccttc aacgtgcttg tcggtcacca gcaggttcag cttacgcttg
      961 gacatcacag tgtattccag attcaggcgc gagaattcgt actggcgcgg atgaacagga
     1021 atggtgatgt tatccagtac ccagtcgtac agacgacggt tgtcctggaa ctcaagcgta
     1081 cacagagagt gcgtaatacc ttccagcgca tcgctgatgc aatgggtgaa gtcgtacatc
     1141 gggtagatgc accacttgtt gccagtctgg tggtgttcag caaatttaat gcggtacagc
     1201 accggatcgc gcattacgat aaatggcgac gccatgtcga ttttcgcacg caggcaggct
     1261 ttaccttctt caaaaccacc ggcacgcatt ttttcgaaca gcgccaagtt ctcttcaacg
     1321 ctgcggtcgc ggtacgggct gtttttaccc ggctgcgtca gggtaccgcg gtattcgcgg
     1381 atctgttccg gtgtcagttc atcaacgtac gccaggcctt tattgatcag ttcgatcgca
     1441 taggcgtgga gctgatcaaa ataatcggag gagtaacgga cgttaccaga ccagtgaaaa
     1501 cctaaccact ctacgtcgtt tttgatcgaa tcgacatact cgatatcttc ttttaccgga
     1561 ttagtgtcgt cgaaacgcag gttgcactgg cctttatagt cctgggcgat cccgaagttc
     1621 aggcagatag atttcgcatg gccaatatgc agatagccat tcggctccgg cgggaaacgg
     1681 gtgtgtactg tgatgtgctt accactggcc agatcttcat cgatgatctg acggataaag
     1741 ttagtcgggc gggcttctgc ctcactcatc gtggattcct caaagcgtaa acaacgtata
     1801 acggcgtatg atcttataag cgggacaggc tgacaactgt tagtttttta aaattgataa
     1861 tctaacaagg attatgggga tgattgatga aaaaaaaagc ggcagagaag atctccaccg
     1921 cttgtcattg ttggatgcga cgctgccgtg tcgcatcagg cataaagcag attacttttt
     1981 gatttcatac agcggtgttt gacccgccac aacatggccc tgagctttaa tgagcaagcc
     2041 actgaaatcg tcgatattgc tgcaaaccac cgggctaatc atcgagcggg cgttagcgtt
     2101 caggtaatcc agatccattt ccagaatcgg ttgccctgcg cttacctgcg caccctcttc
     2161 caccagacgt ttaaagcctt taccttccag cgctacggtg tcgataccca tatggacgac
     2221 gatctccgcg cctttttcgg tttccaggca gaacgcgtgg ttggtgttga agattttcac
     2281 gattgtccct gcggctggtg atacgacgat tttatctgtc ggtttcaccg ccacaccgtc
     2341 acccaccgct ttgctggcga atgcttcgtc aggaacctga tccagtgcca cgacatcacc
     2401 ggtaatcggc gataccagct ccgcgataga taccgcgttt ggtacagcct gcggttttgc
     2461 tacaggcgcg gcagttgccg gagttgcttc agctgacgca gcggctaccg gaccacgggc
     2521 aacgactttc ttcatcgcat cgccgatgga ttctgctttc gcgccaacaa tcacctgaat
     2581 agtctgtttg ttcagtttca ctacaccaga agcacccaga cgtttacaca tcgtatcgtt
     2641 gacgcgggca gagtcagcca cggtcaggcg cagacgggta atacaggcat caatcgcttt
     2701 caggttgtca gtaccgccaa ccgcagcaat atagttggtt gccagttgag tcagaccttc
     2761 ttcagtattg ctgttggctt cttcagtaac gatctcgtct tctttatctt cacgacccgg
     2821 cgttttcagg ttgaacatgc ggataaccaa actgaacacc acgaagtaga tagcgaagaa
     2881 gataacgccc atcaccagca gcatccagac gttctggctg gcggcaggca ggttatacat
     2941 caacgcgtag tcgatagcac ccgcagagaa agagaagccg gcatggatac ccagcagcgt
     3001 tgccacaaac aggctgatac cggtcagcag tgcgtgcagg aggtacagca gcggcgcaag
     3061 gaacatgaac aggaattcca gcggctcagt cacaccggtc aggaacgcag taacagcaac
     3121 agaaagcagc atcccgccaa ccatcggacg acgctctttc ggtgctgcga agtacatcgc
     3181 cagcgccgca cccggcagac cgaacatcat gatcgggaag aagccggaca tgaacatccc
     3241 cgcggtgccg tcaccagcgt agaaacggtt gatgtcgccg tggaaaaccg tacccgccgc
     3301 gttggtgaat tcaccaatct ggaaccaggc gatggtgttc agcacctgat gcagaccggt
     3361 tgggatcagc agacggttga tgaaaccaaa gataccggaa cccagcgcgc ccgcagaaac
     3421 gatccactcg ccgcctgcat ggatagcgtg ctgtaccggc ggccagacgt aaccaaaaat
     3481 ggccgccagc accagacaga agaagccggt ggcgatcggc acaaagcgtt tgccgccgaa
     3541 gaagctcagg aagtccggca gtttaatatc ggaccaacgg ttataggctg cgccaccaac
     3601 cagaccggta atgatacccg ccagtacacc catgttaatt tctgggttga tggtcaccat
     3661 cgctttggtt aacacaaagt aacctaccgc acccgccagc gccgccgcac ctgcgctgtc
     3721 tttcgaccag ctggatgcca caccgatggc gaagattaat gcgaggttat caaaaattgc
     3781 accgcccgcc tgggcaataa acgcaacgtt aagtaaatct ggctgaccga atcgcagcaa
     3841 cagcgccgcc accggcagca ccgcgatagg gagctgtaac gccctaccga gtcgctggaa
     3901 aaaacctaaa atattcatct tattccccct acgagaaccc tatttggctc gtttaaagcc
     3961 gtatttttat tttgctgcaa attttactgc cgatgttctg taatcagatt gttagatcat
     4021 ctgctacaga gtgtgtgaaa atttaattcg catcgcaaat taaacgcgtg tcttttgtga
     4081 gttttgtcac caaatatcgt tatcatcact cccttttact ggctaaacca gaaaacttat
     4141 tttatcattc aaaaaatcaa gtcggattga cgcctgtctg cgcaaatcca ggttacgctt
     4201 aaagatgtct aatccgccaa cggcttacat tttacttatt gaggtgaata atgagactga
     4261 tccccctgac taccgctgaa caggtcggca aatgggctgc tcgccatatc gtcaatcgta
     4321 tcaatgcgtt caaaccaact gccgatcgtc cgtttgtact gggcctgccg actggcggca
     4381 cgccgatgac cacctataaa gcgttagtcg aaatgcataa agcaggccag gtcagcttta
     4441 agcacgttgt caccttcaac atggacgaat atgtcggtct gccgaaagag catccggaaa
     4501 gctactacag ctttatgcac cgtaatttct tcgatcacgt tgatattcca gcagaaaaca
     4561 tcaaccttct caacggcaat gccccggata tcgatgccga gtgccgccag tatgaagaaa
     4621 aaatccgttc ttacggaaaa attcatctgt ttatgggcgg tgtaggtaac gacggtcata
     4681 ttgcatttaa cgaaccggcg tcttctctgg cttctcgtac tcgtatcaaa accctgactc
     4741 atgacactcg cgtcgcgaac tctcgtttct ttgataacga tgtcaatcag gtgccaaaat
     4801 atgccctgac tgtcggtgtt ggtacactgc tggatgccga agaagtgatg attatggtgc
     4861 tgggtagcca gaaagcactg gcgctgcagg ccgccgttga aggttgcgtg aaccatatgt
     4921 ggaccatcag ctgtctgcaa ctgcatccga aagcgatcat ggtgtgcgat gaaccttcca
     4981 ccatggagct gaaagttaag actttaagat atttcaatga attagaagca gaaaatatca
     5041 aaggtctgta attgttatcc ctgccctggt tccttgctca gggcaatatt ttttaaaatc
     5101 ggggggcaga atgtatgcat taacccaggg ccggatcttt accggccacg aatttcttga
     5161 tgaccacgcg gttgttatcg ctgatggcct gattaaaagc gtctgtccgg tagcggaact
     5221 gccgccagag atcgaacaac gttcactgaa cggggccatt ctctcccccg gttttatcga
     5281 tgtgcagtta aacggctgcg gcggcgtaca gtttaacgac accgctgaag cggtcagcgt
     5341 ggaaacgctg gaaatcatgc agaaagccaa tgagaaatca ggctgtacta actatctgcc
     5401 gacgcttatc accaccagcg atgagctgat gaaacagggc gtgcgcgtta tgcgtgagta
     5461 cctggcaaaa catccgaatc aggcgttagg tctgcatctg gaaggtccgt ggctgaatct
     5521 ggtaaaaaaa ggcacccata atccgaattt tgtgcgtaag cctgatgccg cgctggtcga
     5581 tttcctgtgt gaaaacgccg acgtcattac caaagtgacc ctggcaccgg aaatggttcc
     5641 tgcggaagtc atcagcaaac tggcaaatgc cgggattgtg gtttctgccg gtcactccaa
     5701 cgcgacgttg aaagaagcaa aagccggttt ccgcgcgggg attacctttg ccacccatct
     5761 gtacaacgcg atgccgtata ttaccggtcg tgaaccgggc ctggcgggcg cgatcctcga
     5821 cgaagctgac atttattgcg gtattatcgc tgatggcctg catgttgatt acgccaacat
     5881 tcgtaacgct aaacgcctta aaggcgacaa actgtgtctg gttaccgacg ccaccgcgcc
     5941 agcaggtgcc aacattgaac agttcatttt tgcgggtaaa acaatatact accgtaacgg
     6001 actttgtgtg gatgagaacg gtacgttaag cggttcatcc ttaaccatga ttgaaggcgt
     6061 gcgtaatctg gtcgaacatt gcggtatcgc actggatgaa gtgctgcgta tggcgacgct
     6121 ctatccggcg cgtgcgattg acgttgaaaa acgtctcggc acgctcgccg caggtaaagt
     6181 agccaacctg accgcattca cacctgattt taaaatcacc aagaccatcg ttaacggtaa
     6241 cgaggtcgta actcaataag agaaagtatg acaccaggcg gacaagctca gataggtaat
     6301 gttgatctcg taaaacagct taacagcgcg gcggtttatc gcctgattga ccagtacggg
     6361 ccaatctcgc ggattcagat tgccgagcaa agccagcttg cccccgccag cgtaaccaaa
     6421 attacgcgtc agcttatcga acgcgggctg atcaaagaag ttgatcagca ggcctccacc
     6481 gggggccgcc gcgctatctc catcgtcacc gaaacccgca atttccacgc aatcggcgta
     6541 cggcttggtc gtcatgacgc caccatcact ctgtttgatc tcagcagcaa agtgctggca
     6601 gaagaacatt acccgctgcc ggaacgtacc cagcagacgc tggaacatgc cctgctgaat
     6661 gccattgctc agtttattga tagctaccag cgcaagctgc gcgagctaat cgccatttcg
     6721 gtgatcctgc cagggcttgt tgacccggac agcggcaaaa ttcattacat gccgcatatt
     6781 caggtagaaa actgggggct ggtagaagca ctggaagaac gttttaaagt gacctgtttc
     6841 gttggtcacg atatccgtag tctggcgctg gcggagcact acttcggtgc aagtcaggat
     6901 tgcgaagact ccattctggt gcgtgtccat cgcggaaccg gggccgggat tatctctaac
     6961 gggcgcattt ttattggccg caacggtaac gtcggtgaaa ttggccatat tcaggtcgaa
     7021 ccgctgggtg aacgctgcca ctgcggcaac tttggctgcc tggaaactat cgccgccaac
     7081 gctgccattg aacaacgggt gttgaatctg ttaaagcagg gctaccagag ccgtgtgcca
     7141 ctggacgact gcaccatcaa aactatctgc aaagccgcga acaaaggcga tagcctggcc
     7201 tcggaagtga ttgagtatgt cggtcgtcat ctgggtaaaa ccatcgccat tgctatcaac
     7261 ttatttaatc cgcaaaaaat tgttattgct ggtgaaatca ccgaagccga taaagtgctg
     7321 ctccctgcta ttgaaagctg cattaatacc caagcgctga aggcgtttcg tactaatctg
     7381 ccggtggtac gttctgagct ggatcaccgc tcggcaatcg gcgcttttgc gctggtaaaa
     7441 cgcgccatgc tcaacggtat tttgctccag catttgctgg aaaattaatg tgcttttata
     7501 gtggcgctta ttgttgtcaa tattctgggt agtccatgac cattaaaaat gtaatttgcg
     7561 atatcgacgg cgtgctgatg cacgataacg tcgccgtacc gggtgcagcg gaatttttgc
     7621 acgggattat ggataaaggc ctgccgctgg tgttgctgac caactatcct tcgcagactg
     7681 ggcaagatct ggcgaaccgc tttgccactg ccggtgtcga tgtgccggat agcgtgtttt
     7741 atacctctgc gatggcgacc gccgatttcc tgcgtcgcta ggaaggcaag aaagcgtatg
     7801 tggtgggcga aggcgcactg attcatgaac tgtacaaagc cggtttcact attaccgatg
     7861 tgaaccctga tttcgtgatt gttggcgaaa cacgttccta caactgggac atgattcata
     7921 aagcagccta tttcgtcgct aacggtgcac gctttatcgc caccaacccg gacacccacg
     7981 ggcgcggttt ttatcccgct tgtggcgcgt tgtgtgcagg gattgagaaa atctccgggc
     8041 gcaaaccgtt ctatgttggt aagcccagcc cgtggatcat ccgcgcggca ttaaacaaaa
     8101 tgcaggcgca ttcggaagaa acagtgattg tcggcgataa cctgcgtacc gatattctgg
     8161 cgggcttcca ggccggtctg gagacgattc tggtgctttc tggtgtttca tcgctcgacg
     8221 atatcgacag tatgccgttc cgcccaagct ggatttaccc gtcagtcgct gaaatcgacg
     8281 ttatctgaat accacccacg tctgcggacg tggttggctc tgatgacaaa cgccaaactg
     8341 cctgatgcgc tacgcttatc aggcctacgc agctcctgca atatattgaa ttttcatgct
     8401 tttgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaaatcgc actttatcag
     8461 caatttggcc cccgtaaaat ataaccattc aataaaaccc gctaaaaatt atcgattcat
     8521 caataaatac atcctcacac cagacccttt ttcaccaatc gacaatcacc attacgtttt
     8581 ttatttttgc actgccaaat agcttgcgca cagtgctgct ttgcggcatt tttttaaaca
     8641 agcaaacaca acaagcaaca aataccaggt taacggagaa ggttatgtgt tcaatttttg
     8701 gcgtattcga tatcaaaaca gacgcagttg agctgcgtaa gaaagcactc gagctgtcac
     8761 gcctgatgcg tcatcgtggc ccggactggt ccggtattta tgccagcgat aacgccattc
     8821 tcgctcacga acgtctgtca attgttgacg ttaacgcggg ggcgcaacct ctctacaacc
     8881 aacaaaaaaa ccacgtactg gcggtaaacg gtgaaatcta caaccaccag gcattgcgcg
     8941 ctgaatatgg cgatcgttat cagttccaga cagggtctga ctgtgaagtg atcctcgcgc
     9001 tgtatcagga aaaagggccg gaatttctcg acgacttgca gggcatgttt gcctttgccc
     9061 tgtacgacag cgaaaaagat gcctacctga ttggtcgcga ccatctgggg atcatcccac
     9121 tgtatatggg gtatgacgaa cacggtcagc tgtatgtggc ctcagaaatg aaatcgctgg
     9181 tgccagtttg ccgcacgatt aaagagttcc cggcggggag ctatttgtgg agtcaggacg
     9241 gcgaaatccg ttcttattat catcgcgact ggttcgactt tgatgcagtg aaagataacg
     9301 tgaccgacaa aaacgagctg cgtcaggcac tggaagattc cgttaaaagc catctgatgt
     9361 ctgatgtgcc ttacggtgtg ctactttctg gtggtctgga ttcctcaatt atttccgcta
     9421 tcaccaagaa atacgcagcc cgtcgcgtgg aagatcagga acgctctgaa gcctggtggc
     9481 cgcagttaca ctcctttgct gtaggtctgc cgggttcacc ggatcttaaa gcagcccagg
     9541 aagtggcaaa ccatctgggc acggtgcatc acgaaattca cttcactgta caggaaggtc
     9601 tggatgccat ccgcgacgtg atttaccaca tcgaaactta tgatgtgacc actattcgcg
     9661 cttcaacacc gatgtattta atgtcgcgta agatcaaggc gatgggcatt aaaatggtgc
     9721 tgtccggtga aggttctgat gaagtgtttg gcggttatct ttacttccat aaagcgccca
     9781 acgttaaaga actgcatgaa gagacggtgc gtaaactgct ggccttgcat atgtatgact
     9841 gcgcgcgcgc caacaaagcg atgtcagcct ggggcgtgga agcacgcgtt ccgttcctcg
     9901 acaaaaaatt ccttgatgtg gcgatgcgca ttaacccgca ggataaaatg tgcggtaacg
     9961 gcaaaatgga aaaacacatc ctgcgtgaat gttttgagtc atacctgccc gcaagcgtgg
    10021 cctggcggca gaaagagcag ttctccgatg gcgtcggtta cagttggatc gacaccctga
    10081 aagaagtggc ggcgcagcag gtttctgatc agcaactgga aactgcccgc ttccgcttcc
    10141 cgtacaacac gccgacctca aaagaagcgt atctgtaccg cgagatcttt gaagaactgt
    10201 tcccacttcc gagtgccgct gagtgcgtgc cgggcggtcc ttccgtcgcg tgttcttcag
    10261 ctaaagcgat cgaatgggat gaagcgttca aaaaaatgga cgatccgtct ggtcgtgcgg
    10321 ttggcgttca ccagtcggcg tataagtaag aaatgcggta ctgccccgga gaaatccggg
    10381 gctgttttag ttattgcgct gcttgttgat tctgatatgc agtaaggaaa tgttccgggt
    10441 ttgccacatc actgtttgac aactcatcac tgtaggccag tatttcatag attaatttcc
    10501 caccactatt gttgaattcc gaatttcatc ctgtacgaca aaagaatggc tgtagggtgg
    10561 ggaaaaacac aaagtaaaca ataattgacg aatatagcgc cacgctgttc gcaacctaac
    10621 caaacagtca ctttcgagca attttccttg aaaaagaggt tgacgctgca aggctctata
    10681 cgcataatgc gccccgcaac gccgataagg tatcgcgaaa aaaagatggc tacgtagctc
    10741 agttggttag agcacatcac tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc
    10801 accatctttt ttgcgggagt ggcgaaattg gtagacgcac cagatttagg ttctggcgcc
    10861 gcaaggtgtg cgagttcaag tctcgcctcc cgcaccattc accagaaagc gttgtacgga
    10921 tggggtatcg ccaagcggta aggcaccggt ttttgatacc ggcattccct ggttcgaatc
    10981 caggtacccc agccatcttc ttcgagtaag cggttcaccg cccggttatt ggggtatcgc
    11041 caagcggtaa ggcaccggtt tttgataccg gcattccctg gttcgaatcc aggtacccca
    11101 gccatcgaag aaataatctg gctacgtagc tcagttggtt agagcacatc actcataatg
    11161 atggggtcac aggttcgaat cccgtcgtag ccaccaaatt ctgaatgtgt cgaatacgtt
    11221 cggcaaattc aaaaaccaat ttgttggggt atcgccaagc ggtaaggcac cggattctga
    11281 ttccggcatt ccgaggttcg aatcctcgta ccccagccaa tttattcaag acgcttacat
    11341 cgtaagtgca cccagttggg gtatcgccaa gcggtaaggc accggattct gattccggca
    11401 ttccgaggtt cgaatcctcg taccccagcc acattaaaaa agctcgcttc ggcgagcttt
    11461 ttgcttttct gcgttcattc aatgtcgaat gcgatgttga cacgtcttat ccttcaatgt
    11521 cggatgcgac gctgccgcgt cttatccgac ctacggctgg cacgcatccg gcaatgttgt
    11581 aaggctacaa ccctaacgca tatttcagcg cctgacgttt caacacgcca gcacgctctg
    11641 ccgccattaa cccgagatta cgcacaaaac gcagcggcgg cagattatta ctgaatccgg
    11701 cataaaacag atccatacca ctttgcataa tgaagttatc cgccatgcgc cgcatctggt
    11761 aacgcttgag gacaggataa ctggcccacg cttcaccgta gctgcgggca ttcaccagaa
    11821 catcaatcag ggcatcgaca tcacgataac caagattcac cccctgcccc gccagcggat
    11881 ggatggtatg cgcggcatcg cccaccagcg caagccccgg ctgcacatac tgcaacgcat
    11941 gtcggcgcgt cagcggaaac gcaccagcgg caagcggtgt aacgtaaccc agacgcgacg
    12001 ggaaatgctt cgcgatttcc gcctggagtt gcgccatatt catattctgc aactggcgaa
    12061 tacgcgtcgg agagtcatac cacaccagcg atgcccagtt atcaaacaac ggcagaaacg
    12121 cacgcggtcc gtccggggta aattgctgcc aggtgctgtc gccgggatcg ttctcacact
    12181 gtacgctaat caacatgcac gactgcgcat actgccaggc atgaacgcca attcccgcca
    12241 tctgccgcac ctgagaattt gcgccgtcgg caccaatcac cagtttcgcg cggatcgttt
    12301 caccgccctt cagttccagc tcctgaagat cattatctcg atgtaatgca atcagcgagg
    12361 ttggcacacg taacgttact ttcggatgcg cttccagcgc ctgccacaac gcctgttgca
    12421 ggactgtgtt ttccaccatg tagccaagca acggcaactt taattcggct gcgtcaaata
    12481 ccacatgtgc cgtttcccac tcccaggttt ccagtctgcg gtaaggatgg caacgcatag
    12541 cctgtactgc atcccagacc cctaaccctt taagcaatga taccgaagcc gcgctgatcg
    12601 ccgagatccg cacgtccggt tggctgtcag cgacaaacgg tgcaggttct gcgtgctcga
    12661 tcaccgttac cgaaaatccg tgctgtgcca gccccagcgc cagtgcgccg ccgaccattc
    12721 ctccgccgac aatggcaatt tccgttggtt gatttgtcat ggtcaatcat cctgttagca
    12781 atatgcttta agtttacagg atttttaccc ccaagggctg ataacgctca tactggtcac
    12841 aacggcagca aagcattaca ctatgcgccc tgcattcctg gctactattt cgcaagagca
    12901 agtcgatgac caaaaaactc catattaaaa cctggggctg tcagatgaac gagtacgatt
    12961 catcgaagat ggccgatctg ctggatgcct cccacggcta tcaactgacc gacgtggcgg
    13021 aagaagcgga tgtgctactg ctgaacacct gctcaatccg cgagaaggct caggaaaaag
    13081 tcttccatca gttgggtcgc tggaaactgt taaaagagaa gaatccagac ctgattatcg
    13141 gcgtcggtgg ctgcgtggca tcgcaagaag gcgagcacat tcgccagcgc gcccactatg
    13201 tcgatattat ttttgggccg caaacgctgc accgtctgcc ggagatgatc aactccgtgc
    13261 gcggcgaccg cagcccggtt gtagatatca gcttcccgga aatcgagaag tttgaccgtc
    13321 tgccggaacc gcgcgccgaa gggccgaccg cgtttgtctc catcatggaa ggctgcaata
    13381 aatattgcac ctactgcgtg gtgccttaca cccgtggtga agaggtaagc cgtccgtctg
    13441 acgatattct gtttgagatt gcccagcttg cggctcaggg cgtgcgtgaa gtcaacctgc
    13501 tcggtcagaa tgtgaatgcc tggcgtggtg agaactacga cggcaccacc ggatcgtttg
    13561 ccgatctgtt gcgtctggtt gctgcgatcg acgggatcga tcgtattcgc tttaccacca
    13621 gccatccgat cgaattcacc gacgatatca tcgaagtgta ccgcgacacg cctgagctgg
    13681 tgagcttcct gcatctgccg gtacagagcg gttccgatcg cattctgaac ctgatgggac
    13741 gtacccatac ggcgctggag tacaaagcga tcatccgtaa actgcgtgcg gcgcgtccgg
    13801 atattcagat cagttctgac ttcattgttg gcttccctgg cgaaaccacc gaagacttcg
    13861 agaaaacgat gaagctgatt gccgacgtca atttcgacat gagctacagc tttatcttct
    13921 ctgcacgtcc aggaacgcct gccgccgata tggttgatga tgttccggaa gaagagaaga
    13981 agcagcgtct gtatattctg caagaacgta tcaatcagca agcgatggca tggagccgtc
    14041 ggatgctcgg caccacccag cgtattctgg tagaagggac ttcgcgtaag agcatcatgg
    14101 agctttccgg gcgtaccgaa aataaccgcg tggtcaactt cgagggcaca ccggatatga
    14161 tcggtaaatt cgtcgatgta gaaattaccg acgtctaccc gaactctctg cgcggtaaag
    14221 tggtgcgaac tgaagatgaa atgggtctgc gcatggcaga aacaccggag tcggtgattg
    14281 cccgcacccg caaagaaaac gaccttggcg tgggttatta tcagccgtaa ttctcaggcc
    14341 tgccgttccg gtgggccttg ttttttctct ctttatcccc atctttatcg caatgcttgc
    14401 gcctttttcc tgtggcgtga ataatttcct tataggccgg tgaatgttca gcttccggcg
    14461 gcatacagat aagaggaacg gtttgaacat agacactcgc gaaatcaccc tggagccagc
    14521 agacaatgcg cgtctgttga gcctgtgcgg cccgtttgat gacaacatca agcagctcga
    14581 acgccgtctc ggcatcgaga tcaatcgccg cgataaccac tttaaactga ccggccgtcc
    14641 gatttgcgtc accgctgcgg cagacattct gcgtagcctg tatgtcgata ctgccccgat
    14701 gcgcggtcag attcaggata tcgaaccgga acagatccac cttgcgatta aagaagcgcg
    14761 ggtactggag caaagcgcgg agagcgtgcc ggagtacggc aaagcggtca atatcaaaac
    14821 taaacgcggc gtaattaagc cgcgcacgcc aaaccaggcg cagtacatcg ccaatattct
    14881 cgaccatgac attaccttcg gcgttggccc ggcgggtacg ggtaaaacct acctggcagt
    14941 ggctgcggca gttgatgccc tggagcgtca ggaaattcgc cgtattctgc tgactcgtcc
    15001 ggcggtcgaa gccggtgaga aactgggctt cctgcctggc gatttaagcc agaaagtaga
    15061 cccgtatctg cgcccactgt acgacgcgct gtttgaaatg ctgggctttg agaaagtcga
    15121 gaaactgatt gagcgcaacg ttattgaagt cgcgccgctg gcctatatgc gtggtcgtac
    15181 gctgaacgac gcgtttatca ttctcgatga gagccagaac actaccatcg aacagatgaa
    15241 gatgttcctg acccgtatcg gttttaactc aaaagcggtt atcaccggcg acgtcacaca
    15301 gatcgacctg ccgcgtaata ctaaatcagg cttacgtcat gccattgaag tgctggccga
    15361 tgtcgaagag atcagcttta acttcttcca cagcgaagac gtggttcgtc acccggtggt
    15421 ggcgcgtatc gttaacgctt atgaagcctg ggaagaagca gaacaaaaac gtaaagcggc
    15481 gctggcagca gaacgcaagc gcgaagaaca ggaacaaaaa tgagtcaggt gatcctcgat
    15541 ttacaactgg catgtgaaga taattccggg ttaccggaag agagccagtt tcaaacatgg
    15601 ctgaatgcgg tgatcccgca gtttcaggaa gagtcggaag tgactattcg cgtggtcgat
    15661 accgccgaaa gccacagtct gaatctgacc tatcgcggta aggataagcc gaccaacgtg
    15721 ctctccttcc cgtttgaagt gccgcctggc atggagatgt cgctactggg cgatctggtt
    15781 atctgccgtc aggtggttga gaaggaagct caggagcaag gcaaaccact ggaggcgcac
    15841 tgggcgcata tggtggtgca cggcagtctg catttgttag gttacgatca cattgaagat
    15901 gacgaagcag aagaaatgga agccctcgaa acagagatta tgcttgctct gggctatgag
    15961 gatccgtaca ttgccgagaa agaataattt gccaggcatt tgactggcag acgtccccgc
    16021 cgttggtgat taacgacggc gttttgatta actaacatga gaacccatac gacgccatga
    16081 gcgacgacaa ttcacacagt agtgacacga taagcaacaa gaagggattt ttctccctgt
    16141 tactcagcca acttttccac ggtgaaccga aaaaccgtga cgaactgctg gcgctgatcc
    16201 gtgattccgg gcagaacgac cttatcgacg aagatacgcg cgatatgctc gaaggggtga
    16261 tggacatcgc agaccaacgc gttcgcgaca tcatgatccc ccgctcccag atgattaccc
    16321 tgaaacgcaa ccagacgctg gacgaatgcc ttgatgtcat catcgagtcc gcccactcac
    16381 gtttcccggt gattagcgaa gacaaagatc acattgaagg gattctgatg gcgaaagact
    16441 tgctgccgtt tatgcgcagc gatgctgaag ccttcagcat ggacaaagtg ttacgtcagg
    16501 cggttgtcgt tcctgaaagt aagcgcgtag accggatgct gaaagagttt cgctctcagc
    16561 gttaccacat ggcgatcgtt attgacgaat tcggtggggt ttccggtctg gtgaccattg
    16621 aagacatcct ggaactgatt gttggtgaga ttgaagacga gtatgacgaa gaagatgata
    16681 tcgacttccg tcagctgagt cgtcatacct ggaccgtgcg cgcactggct tccattgaag
    16741 acttcaacga agcgttcggc acccacttta gcgatgaaga agtcgacact atcggtggtc
    16801 tggtgatgca ggcgtttggg catcttccgg cgcgtggcga aactatcgac atcgacggtt
    16861 accagttcaa agtggcgatg gccgatagtc ggcgtattat tcaggttcat gtcaaaatcc
    16921 cggatgactc accccagcca aagctggatg aataaaaccg aaactggata gataactaca
    16981 tggattttgc ctcattaatt gaacgccagc gcattcgcct gctgctggcg ttattattcg
    17041 gtgcctgcgg aacgctggcc ttctctcctt acgacgtctg gcctgcggcg attatttcgc
    17101 tgatggggct tcaggcgttg acctttaacc gtcgcccact ccagtctgcc gctattggct
    17161 tttgctgggg atttggcctc tttggcagcg gtattaactg ggtctatgtc agcatcgcga
    17221 cctttggcgg gatgcctggc ccggttaaca tcttcctggt ggtgctgctg gcggcgtatt
    17281 tgtcgctgta taccggactg tttgctggcg tgctgtcgcg tctgtggccg aaaaccacct
    17341 ggctgcgcgt ggcgattgcc acccctgctc tctggcaagt gaccgagttt ctgcgcggtt
    17401 gggtgctgac tggcttcccg tggttacagt tcggctatag ccagattgat ggcccgttaa
    17461 aagggctggc accgctaatg ggcgtggaag ccattaactt cctgctgatg atggttagtg
    17521 gcctgctggc actggcgttg gtcaaacgca actggcgtcc gctggtggtg gccgtcgtgc
    17581 tgtttgccct acccttcccg ctgcgttaca tccaatggtt taccccgcaa ccggagaaaa
    17641 ccattcaggt ttcgatggtt cagggcgata ttccgcaatc gctgaaatgg gacgaaggcc
    17701 agcttcttaa tacgctaaag atttactaca acgcaacggc accgctgatg ggcaaatcat
    17761 cgttgattat ctggccggag tcggcgataa ccgatctgga aattaatcag caaccgttcc
    17821 tcaaagcact ggacggtgag ttgcgtgata aaggtagctc gctggtaacc gggattgtcg
    17881 acgcgcgtct caataagcag aaccgctacg atacctacaa caccatcatc acgctgggta
    17941 aaggtgcacc gtacagctac gaatcagccg atcgctataa caaaaaccat ctggtgccgt
    18001 ttggcgagtt tgtcccgctg gagtcgattc tgcgtccgtt agcaccgttc tttgatctgc
    18061 cgatgtcgtc gttcagccgt gggccatata tccagccgcc gctgtcggca aatggtattg
    18121 agcttactgc ggctatttgc tacgagatta ttctcgccga gcaagtgcgc gataacttcc
    18181 gcccggatac cgactacctg ctgactatct ccaacgatgc gtggtttggt aaatctattg
    18241 gtccatggca acacttccag atggcgcgaa tgcgtgcgct ggagctggcg cgcccactgt
    18301 tgcgcagcac caacaacggc attacggcgg tgattggccc gcagggtgag attcaggcga
    18361 tgatcccgca gttcgcccgc gaggtgttaa ccactaacgt gacgccgacc accggactca
    18421 caccatacgc acgtaccggc aactggccgc tgtgggtgct gacggcactg tttggttttg
    18481 ctgctgtgtt gatgagtctg cgtcagcgac gtaaataatc ccttcttatc gtgccggaat
    18541 acatcccata tttcggcacg ctcaccggat gcggcatcag tgcattatcc ggataacaat
    18601 attctcctat ccaactttgg cacatctatt gctttgttat acaaggcaaa gcttgaacca
    18661 gcatcagtac aacccggttg caccacagca atccaccggt agcaccaaaa gagtgcattt
    18721 taagaatttt gcttcagaat ggtgcggtgc acctcgcaaa aacaaacaat aaaacatcaa
    18781 tatctttaca tttagccaaa ctaaatgtta cctaacaggc acaacactgc acaataaagt
    18841 tgcagacgat aacaacacaa acactcacaa cgggtatcca tgcgttctta acgcagaaga
    18901 taaaggtgtt ggatatgcaa ttacgtaaac ctgccacagc aatcctcgcc ctggcgcttt
    18961 ccgcaggact ggcacaggca gatgacgccg ccccggcagc aggcagtacg ctggacaaaa
    19021 tcgccaaaaa cggtgtgatt gtcgtcggtc accgtgaatc ttcagtacct ttctcttatt
    19081 acgacaatca gcaaaaagtg gtgggttact cgcaggatta ctccaacgcc attgttgaag
    19141 cagtgaaaaa gaaactcaac aaaccggact tgcaggtaaa actgattccg attacctcac
    19201 aaaatcgtat tccactgctg caaaacggca ctttcgattt tgaatgtggt tctaccacca
    19261 acaacgtcga acgccaaaaa caggcggctt tctctgacac tattttcgtg gtcggtacgc
    19321 gcctgttgac caaaaagggc ggcgatatca aagattttgc cgacctgaaa ggcaaagccg
    19381 tagtcgtcac ttccggcact acctctgaag ttttgctcaa caaactgaat gaagagcaaa
    19441 aaatgaatat gcgcatcatc agcgccaaag atcacggtga ctctttccgc accctggaaa
    19501 gcggtcgtgc cgttgccttt atgatggatg acgctctgct ggccggtgaa cgtgcgaaag
    19561 cgaagaaacc agacaactgg gatatcgtcg gcaagccgca gtctcaggaa gcctacggtt
    19621 gcatgttgcg taaagacgat ccgcagttca aaaagctgat ggatgacact atcgctcagg
    19681 tgcagacctc cggtgaagcg gaaaaatggt ttgataaatg gttcaaaaat ccaattccgc
    19741 cgaaaaacct gaacatgaat ttcgaactgt cagacgaaat gaaagcactg ttcaaagaac
    19801 cgaatgacaa ggcactgaac taattacaag aaccaggggc ggaaattcca gccctctcga
    19861 ttgttacgta gcacggacag actatacgcc tgatggtcgt tccccatcgg gcctgaaaac
    19921 cgcaatacgc tgggtaacaa tcttcgaggg tagcagttaa cgctgctacc ctcttttttc
    19981 tggagtagat ttatgtctat agactggaac tggggtattt ttttacaaca agccccgttc
    20041 ggcaatacca cctatcttgg ttgggtctgg agtggttttc aggtgacgat cgctttgtcg
    20101 atctgcgcct ggattatcgc tttcctcgtc ggttcatttt tcggcatttt acgtaccgtt
    20161 ccaaaccgtt ttctctccgg tctgggtacg ttgtatgtcg aactgttccg caacgtgccg
    20221 ttgattgtgc aattctttac ctggtatctg gtgatcccgg agctgctgcc ggagaaaatc
    20281 ggcatgtggt ttaaggccga gctggatccc aatattcagt ttttcctttc atccatgctc
    20341 tgcctggggc tgtttaccgc cgcccgtgtt tgcgaacagg tacgcgccgc tattcagtcg
    20401 ctaccgcgtg ggcagaagaa tgccgcgctg gcgatgggcc tgacgctacc gcagacttat
    20461 cgatacgtac tgctgcctaa tgcttatcgc gttatcgtcc cgccgatgac ttccgagatg
    20521 atgaacctgg tgaaaaactc cgccatcgcc tcgaccatcg gtctggtgga tatggcggcg
    20581 caggcgggta aattgctcga ctactcggct cacgcctggg agtcgtttac cgccatcacg
    20641 cttgcgtatg tgttgattaa cgcttttatc atgctggtga tgacgctggt tgaacgtaaa
    20701 gttcgcctgc ccggcaacat ggggggcaaa taatgtacga gtttgactgg agttccattg
    20761 ttccttccct gccatatctg ctcgacgggc tggtgatcac cctgaaaatc accgtcacgg
    20821 cagtcgtgat tggtattttg tggggtacca tgctggcggt gatgcgcttg tccagcttcg
    20881 cgcctgtttc gtggtttgcc aaagcctacg ttaacgtatt ccgctcgatt cctttagtca
    20941 tggttttgct gtggttttac ttgattgtgc cgggatttct gcaaaatgtg ctgggattat
    21001 cgccaaaaaa tgatattcgt ttaatttcgg cgatggtggc gttttcgatg tttgaagcgg
    21061 cctactattc agagattatc cgtgccggta ttcaaagtat ttcccgtggt cagtcgagcg
    21121 ccgcgctggc gttggggatg actcactggc agtcgatgaa actgattatt ctgccgcagg
    21181 ctttccgcgc gatggtgccg ctgctgctca ctcagggcat cgtactgttc caggatacct
    21241 cgctggtgta tgtgttatct ctggccgatt tcttccgtac cgcctcaacc attggtgagc
    21301 gtgatggtac gcaggtcgag atgatcctgt ttgccggatt tgtttatttc gttattagcc
    21361 ttagcgcgtc gttgttggtc agctacttga aaagaaggac agcataatga ttaccctgaa
    21421 aaatgtttca aaatggtatg gtcactttca ggtgctgacc gactgctcaa ccgaagtgaa
    21481 aaaaggcgaa gtggtggtgg tttgcggccc gtctggttcc ggcaaatcaa cgctgattaa
    21541 aaccgtcaac ggcctcgaac cagtgcagca aggtgaaatc accgtcgatg gtatcgtggt
    21601 taacgacaag aaaaccgatc tggcaaagct gcgctcccgc gtcgggatgg tattccagca
    21661 tttcgagctg ttccctcatc tgtcgattat cgaaaacctg accctggcgc aggtgaaagt
    21721 gcttaaacgc gataaagcgc ccgctcgtga aaaagcattg aaattgctgg agcgcgtggg
    21781 actttccgca catgccaata aatttccggc gcagctttcc ggcggtcagc agcagcgcgt
    21841 ggcaatcgct cgcgcattgt gtatggctcc tattgcgatg ctgttcgacg aaccgacatc
    21901 ggcgctggat ccggagatga tcaacgaagt gctggacgtg atggtggaac tggcgaacga
    21961 aggaatgacc atgatggtgg tgacccacga aatgggcttt gcccgcaaag tggcgaatcg
    22021 ggtgatcttt atggacgagg gtaaaattgt cgaagactcg ccgaaagacg ctttcttcga
    22081 tgatccgaaa tcggaccgcg caaaagactt cctcgcgaaa atcctgcatt aatcactctg
    22141 gcgcacatac ccaattgcgc gccatctcac gctttatcgt ttcgcccctc gtatctgtta
    22201 catcctggcg ttacccttgt cgcaaagaag cacacaacaa ggagcaacaa tggcactgcc
    22261 aattctgtta gattgcgacc caggtcatga tgacgctatc gcaatagttc tcgcactcgc
    22321 ctcaccagag cttgatgtca aagcaattac gtcttccgcc ggaaaccaga caccagaaaa
    22381 aaccttacgc aatgttctgc gtatgctgac cttgcttaat cgcaccgata ttccggtggc
    22441 aggcggcgcg gtaaaaccgt taatgcgtga gttgattatc gcggacaatg tgcacggcga
    22501 aagcggtctc gacggcccgg cattaccgga accgacgttc gcaccgcaaa actgtacggc
    22561 ggtggagctg atggcgaaaa cgctgcgcga aagtgcggaa cctgtcacga ttgtttctac
    22621 cggaccgcaa actaacgttg ccttgctgct caatagccat ccggaactgc atagcaaaat
    22681 tgcccgtatc gtgattatgg gcggcgcaat ggggcttggt aactggacgc ctgcggctga
    22741 atttaatatt tacgttgacc cggaagcggc agaaattgtc ttccagtcag ggatcccggt
    22801 ggtgatggcc ggtctggatg ttactcataa agcgcaaatc cacgttgaag acaccgagcg
    22861 tttccgcgcg attggcaacc ctgtttcaac cattgttgcc gaactgctgg atttcttcct
    22921 cgaatatcat aaagacgaaa aatggggctt tgtcggcgca ccgctgcatg acccatgcac
    22981 catcgcctgg ctgttgaaac ccgagttgtt taccactgtt gagcgctggg ttggcgtgga
    23041 aacacagggg aaatataccc agggtatgac ggttgttgat tattattatc tgactggcaa
    23101 taaaccgaat gccactgtaa ttgtcgatgt tgatcgtcag ggctttgttg atttactggc
    23161 tgatcgtctg aaattttacg cttaacactc gtcattgatt aagatcacca cgcgttacct
    23221 gggtggtctt aatctattta tctctttttc tttcaagata taaatgcaat ggataatgca
    23281 gaactcgcca taggtatcga tctcggcact accaatagtt taattgccgt ctggaaagac
    23341 ggtgccgcgc aattaattcc aaataagttc ggtgaatatt taacaccatc cataattagc
    23401 atggatgaaa ataatcatat tttagtcgga aaaccggctg tatcacggcg tacttcgcat
    23461 ccggataaaa cggcagcgtt atttaaacgt gcaatgggca gtaataccaa ctggcggtta
    23521 ggcagcgaca catttaacgc gccagaactg tcctctttgg tattacgctc attaaaagaa
    23581 gatgccgaag aatttctgca acgtccgatt aaagatgtgg tgatctccgt tctggcttat
    23641 ttcagcgatg aacaacgcaa gcatacccgt ttagcagcgg acttagccgg gttaaatgcg
    23701 gtacgcttaa ttaatgaacc cacagcagct gcgatggcgt atggcctgca tacccaacaa
    23761 aatacccgtt cgctggtgtt tgatctcggt ggcggcacgt ttgacgttac ggtgcttgag
    23821 tacgccacgc cggtgattga agttcacgcc tccgctggcg acaacttcct cggtggcgaa
    23881 gattttaccc atatgttggt cgatgacgtt ttaaagcgcg cggatgtcac aaggaccacg
    23941 cttaacgaga gtgaactggc agccttgtac gcctgtgtgg aagcggcaaa atgtagcaat
    24001 caatcgccaa tgcacattcg ctggtagtac caggatgaaa cgcgggaatg cgaattttac
    24061 gagaacgaac tggaagattt gtggttgccg ctgctcaatc gcttgcgagt gccgattgaa
    24121 caggcgttgc gcgatgcgcg tctgaagccg agtcaaatcg acagtctggt gctggttggc
    24181 ggcgcgtcac aaatgccgct ggtgcagcga atcgccgtgc gtctgtttgg caaattaccg
    24241 tatcaaagtt acgatccgag caccattgtc gcgctgggcg cagcaatcca ggccgcctgc
    24301 cgcttacgca gtgaagatat tgaagaagtg atcctcactg atatttgccc ttactcgttg
    24361 ggcgttgagg ttaaccgcca gggcgtttcc ggcattttct cgccgattat tgaacgaaac
    24421 accactgtgc ccgtgtcgcg tgtagaaact tattcaacca tgcacccgga acaggattca
    24481 attacggtta acgtctatca gggagaaaac cacaaagtta aaaacaacat tctggtggaa
    24541 tccttcgatg tgccgttgaa gaaaaccggg gcttatcagt cgattgatat tcgctttagt
    24601 tatgatatca acgggttgct tgaagttgac gtgcttctgg aagacggcag cgttaagtcc
    24661 agagtgatta accacagccc ggtaacattg agcgcgcagc agattgaaga gagtcggacg
    24721 cggttatccg cattgaaaat ttatccgcgc gatatgctca tcaatcgcac ctttaaagcc
    24781 aaactggaag agttgtgggc gcgggcgctg ggtgacgagc gagaagagat tggccgggtg
    24841 atcaccgatt ttgatgcggc gctgcagtca aacgatatgg cccgcgtcga tgaggtcagg
    24901 cggcgggcga gcgtttattt agccattgag acctcataat cacggctgcc ggatgcggcg
    24961 taaatgctgc atccggcatc ggcgcaaaaa acacatcgtc aactaaacaa catcgcgatt
    25021 ttgccgagag cataacctat aaccagcacc gttaaactga ttttgataat gctggtaatg
    25081 ctctgtggtt tttcaccctc atcatttttt tcttcatggt tttcaggtgt tggctcatcg
    25141 acatcttcat cctccggctc gagttttact ttcgtcaaca gcatccgggt gataatggcc
    25201 tctaatggat gagcatctcc gccttccagc cttgtattaa gacgggccag catctcttgt
    25261 ttcggaaacg cttcaagtac tacctgttcc gctacccgat acaaatccag taacggtgaa
    25321 tcttcttctg tctcgctacc gactctcgat ttccaccacg cccatgcttt gcgtttgaag
    25381 aataacgact caataaacga ttgacctaac ggcgataatt ttccgctatg taaaatttcc
    25441 gcccataaca gccgcgtccg ggcgctctca ccaggaacat atttcagcgg ttgaccttgc
    25501 gcatctacca tgctccgcgc ttcgataacc atcactaata acgcgaaata atccgggcaa
    25561 tgacgcttgc tccactgcaa caacaaatta tcagcttgtg tagatgggta actttcccag
    25621 tacgcgccga gatcagcaag caggctttcc ccctcgccgc agtacaaacg ttgtgcacac
    25681 tggtaatacc gggcatcttc gctttctggt tcttccgctt gccactgttt ggctacagga
    25741 atgagttcca ccatacctgt ttgcaccgag ctgtaccagc gcaacagttt gcgatgtaac
    25801 tttgcatcat caggaatgaa ccacggtcct tccatcgcca aaaacgcagt gacatagtca
    25861 ggatggtaac ggcagcaacg ttccagcgcg aagtaatatt caacagtctg gttctgtaac
    25921 gcgacgggat attccagaag gctaaggaaa tcgaaacaat caccagcctg aatggcctca
    25981 agaaaagcct ccctttcttc ctcatccatt ccttcgctgt cattaaacga atgccagttc
    26041 aaacgctctg ccaacaaaga agcacaattt aatgacagat atcgcgcttc tatagcgatt
    26101 gcacacagcg gccagcgtaa ttgatcgaca tcctccatgt tccaggtatt taattgctga
    26161 ataaatttct gccaggcgga gggctgaaaa cgatcacttt ctgaatccag taatgtcctg
    26221 aatgcacgta gtatttcatg ttcagcggtg gcatcttttt cttcatcatc tgcttcctcg
    26281 acaggattta ccgccagtcg cagcgcctct tcataggcct ggcgtaatgc tttaaacccc
    26341 tgcggatcgg tttcgggatg acacaacggt aagcgagcaa gataagcctg gcggataatg
    26401 tctatttgcg tcgtgctttc aatttcgagg atttgccaac atgttttcat aggtcgctca
    26461 catccttaag cgttaccggg cattcaggct ggacagttaa tggctgccag ggaagcgtgg
    26521 catagaaacg gtcgcttccc gctgccagct cttcgaacat ccgactcccg gcgctatcgt
    26581 atttgtacag ggttagcgcc tcgcgtagcg aagcgttgtc ttcgcactga gaagattgcc
    26641 agtagagacg ccctagtgaa taggcagaaa aatagtgcat ccagctgtga tagtaatggt
    26701 gagctcgtgc atagacgcgc gattgcagcc ataaactctc ttcttccgtc agccagttgt
    26761 tgagcacacc ggcccgtgac agaaatccca ttcgaacata atcccaggct ttgattcccc
    26821 cttctccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
    26881 agccaccagc aattaatgct ttccactctt caggggaaga gcgttgccat gtcagatata
    26941 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
    27001 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttatc ccggtataca
    27061 tttttggcga gtcatgcgcg ggtttgcaac tctgattaag tatttccatg ggcgcagaaa
    27121 gcgcaaaaag aagatattgt tcttccttat tcatttttta ataatccctt aagtgcatta
    27181 actgactact tttttattat tgatgagtat aaacttgacg ctttcaaaat aaaaagaaaa
    27241 tcgaagcatt cacacatgaa taaaaaatta atgtatatat tcgcaatttt tatagttgca
    27301 gcaattacct gtattagcca acccaagaaa acgatgttgc gtgataaagc catggtgaat
    27361 tatgcctttg attatttaag atcaccaggc agtctaccgt taaccacggc agccacggag
    27421 ctttccgcga ttcatggtca ctcaacatcg caatatcgcc ttggcgaatt ttatcttcat
    27481 ggtagcgacg gtaaaccact ggattataca caggcgagat actggtatga gcaatcagca
    27541 gaacaggaaa atccgcgcgc acaaagcaaa ctggggtgga tctacctgaa aggcctggga
    27601 gtcaaacccg atacccgtaa agcaattctc tggtataagg aagcagctga acaagggtat
    27661 gctcatgctc aatatacttt gggtttgatc tacagaaatg gcactggtat taatgttaac
    27721 cattatgaat ctcaaaaatg gttaaaactg gccgccaaac aacattacaa aaatgcggaa
    27781 agattacttg ccggacttcc cgcacattaa cgtcgactgt cacccgaaga gatggagtat
    27841 tttccctatc aaaccggcaa agataaaaat gtagaaaata attttgatga cgcccagaca
    27901 gcccagcccg ccatcatcaa cgggcattgg ctgcggttgc ggctccggct cgacggtttc
    27961 ctgaccggta aaattcgccg cctgaattaa taatcgacga ataattaatg cttccagtgg
    28021 acaacgatct tctgcatcaa ggcggctcac aatcggcatt aatgattctt ctgtcggttg
    28081 gctggcctgc actaattgtt tggcaactaa atataatggc cataagggat gttctgtatc
    28141 acgcgtgagt gagatccgcg gcttacgtcc tttgtgcaaa accatctcga ccagtgaacg
    28201 ccccagcgca gagagttttt catcgctgag tatttcgtat aaatgaaaac gcgtacgggc
    28261 gctgtcgccg gggacataaa gtagcggttt tccctgatcg ttaaccagat cacgcgcttc
    28321 aatcatcatc accactaatg ggtaataata gacccgatgc tgacggcacc attgcaacat
    28381 taaagcatcc gcctgggtgg agggatactc gcgccagtag taacaaagtt ctgccagcag
    28441 gctttcgcct tctccacaat agacgcgttg tgcgtattca taataacgcg catcttcatt
    28501 atcaggttca gcttcccgcc acgaccgggc gtaatccagg agttcgggga ttcccaggcg
    28561 gcttgcgcta taccagcgga gtaaatttct gtgcagtttt gcatcatcgg ggataatcac
    28621 tgcgcgatgt tgcgctaaat acaatgtcag ccattctgga tgcgatgacc aaatccgagc
    28681 gaacatttgg taaaaatcga tggtgtcatt ttgcacggca actggcagat gcagaatggt
    28741 ctggaagtta aaaacattcc ccttcgcaat ggcatataaa aaggattcca gttcttcttc
    28801 gtctatttcc ccgacgtttt cctcctgcaa ccaccgcaat ctttctgcta ataacaccac
    28861 gcactcgaag gataaatggg cagtgttcat ggcgattgtg cacagcgacc agcgtaattc
    28921 atcaatctct tccatcgagc aataatttaa ttgctgaatg aatcgctgcc aggcggaggg
    28981 cagaaaacgt tcgctatcag aggcaagtaa cgcgcgaaag gcgagcagaa tttcatgttc
    29041 tgctacctca tattcttctg gttgccaaac agatttagca ggcgactgcg caatccgtag
    29101 cgcctcctca tacgcctgac gaagttgttt aaaaccctgc ggatcggttt ctggatgaaa
    29161 ggacggtaac agcgccagat aagcgcggcg gataatatcg acgtcagtcg tttcctctat
    29221 atcgaggatc ttccagcaat ttttcataag tcgctcatat ccttaagcgt ttccgggcat
    29281 gtggggtagt cagccagtgg ttgccaggac aaggtagggt aatacgcatc atcactggca
    29341 aataattgat aaaacatatc attgccggaa tcgtcgtatt ctttacgcgc taagaattcg
    29401 cgtaataaag gcagatgatc atcttcggga gactgccaat attgccgacc aaaggtataa
    29461 ccggcaaagt attgccgcca gttactataa taacgtagag cccgtagatg aatacgcgac
    29521 tggatccata gactttcttc ttcactcaac cagttattaa gtacgcccat tcgacttaaa
    29581 aaacccatcc ggacataatc ccatgcttta attcctccct caccacagca ttcggctgtg
    29641 ctggcgacaa attgcgcata agcctgacct tgctcattta attcagcaag atagtcacgc
    29701 cattcacatg gagaataacg aaaccagcgg tgataaaacc ctgccaaccg agcggcatga
    29761 ccgttctcga tcattcggtg aattatgcgc agcaggtcat cacggtcatc aattccccat
    29821 gaggaagaaa ggtcaaaaaa gcatgtacct aaatacattt tgggggatga atgcgaaggt
    29881 aaacagcatt cattgcgtat ttccatcgga gtggaaagtg caaacaatat tttttgcgac
    29941 cccatgtcca tataatttat ccttaaacat tcatttaatg aatgtaaact acatttcgag
    30001 gctcagttat ttatactttt ctgacgcaac ataatacgca taaaataagg cttacgcaaa
    30061 aatggaacac ataaaaatat aatatatttt atctacaacc ataaggaaat gataatgatt
    30121 tttacgtcaa gttgctgcga taatttatca atagatgaga ttatcgaacg tgctgaaaaa
    30181 ggcgattgcg aggctcaata tattgttggg ttttattata atcgcgatag cgcaattgac
    30241 tctccggacg acgaaaaggc cttttactgg ctgaagctgg ccgctgagca aggtcattgt
    30301 gaagcacagt attccttagg gcaaaagtat accgaggata aaagccgtca taaagataat
    30361 gagcaagcca tcttctggct gaaaaaagct gccctacaag gccatacttt cgcttccaac
    30421 gcccttggct ggatactgga tcgtggagaa gaccccaact ataaagaagc ggttacctgg
    30481 tatcagatag ccgcggagag cggaatgtct tatgcgcaaa ataatcttgg atggatgtac
    30541 agaaatggca acggagtcgc acaagactat acgctggcat ttttctggta caaacaagct
    30601 gcattacaag gccatagtta cgcacaagac aatctggccg atctttataa agacggagaa
    30661 ggcgttgctc aaaacaagac actcgccgca ttctggtatt tgaaaagcgc acagcagggt
    30721 aatcggcacg cccagtttca aattgcatgg gattataacg ctggcgaagg ggtggaccag
    30781 gactataagc aagcgatgta ctggtatctg aaggctgccg ctcaggaaag cgtcggcgct
    30841 tacgtcaaca tcggttatat gtataaacac ggacaaggcg ttgagaaaga ttatcaggct
    30901 gcctttgaat ggtttacgaa agccgctgaa tgcaatgacg ccactgcctg gtataacctg
    30961 gccattatgt atcactacgg agaaggaaga cctgtcgatc tccgacaggc tctcgacctg
    31021 tatcgtaaag ttcagtcatc cggaaccaga gatgtcagtc aggaaattcg tgagactgaa
    31081 gctttactgt agaaataaaa acaagggccg gggaatttca ttctgcccat attctcttta
    31141 ttgctgtgcg aaatcagact attacggctc aaacgggcgt ctctggaact ggtcataacc
    31201 acatttcggg catagcgtca gcacttccgg tgtgtagatc gggagatgga agtgacattt
    31261 ctcgcagacc agatttccca gcccgaccac ttctccgctg tgataaaccc catgatgatt
    31321 gaggtcctgg aaaatttcgc gccattcaag ctgcgtttta tcggtgatgt ctgccagctc
    31381 ctgccacaag ctttctttaa tcacccgcat aaagacgctg tcagattctt ctttcaggct
    31441 ctcttcatag ctcatggcga actcttccag gtcacgtctg acagctcgcg tcagctcatc
    31501 gatctcggtt cgcgttaact cccctgtttt tattacgcgc tcgcgcgcct gttccaccag
    31561 tgcgtcgata tcacgttcgc cattgcgcag gcgttcgttc agtgacgcga ccagttcacg
    31621 gtaatattga gcaaccttgt tcatcgtttc atctcccggg tcgttaactg actataataa
    31681 tagacgttct ttgttccttg ctttgctaat acgcccacaa actctgtaaa ttaatgtacg
    31741 aataatccgg gcatcatttc aacgtcgagt gcgcgaaagg ctgttttgac gcggacgttt
    31801 gggctatgct atgcggatct gaaaaaccac ctcaacgcta catttgtagc cgtattgaaa
    31861 acaggaccac tggctgccat gcaagagcaa taccgcccgg aagagataga atccaaagta
    31921 cagcttcatt gggatgagaa gcgcacattt gaagtaaccg aagacgagag caaagagaag
    31981 tattactgcc tgtctatgct tccctatcct tctggtcgac tacacatggg ccacgtacgt
    32041 aactacacca tcggtgacgt gatcgcccgt taccagcgca tgctgggcaa aaacgttctg
    32101 cagccgatcg gctgggacgc gtttggtctg cctgcggaag gcgcggcggt gaaaaacaac
    32161 accgctccgg caccgtggac gtacgacaac atcgcgtata tgaaaaacca gctcaaaatg
    32221 ctgggctttg gttatgactg gagccgcgag ctggcaacct gtacgccgga atactaccgt
    32281 tgggaacaga aattcttcac cgagctgtat aaaaaaggcc tggtatataa aaagacttct
    32341 gcggtcaact ggtgcccgaa cgaccagacc gtactggcga acgaacaagt tatcgacggc
    32401 tgctgctggc gctgcgatac caaagttgaa cgtaaagaga tcccgcagtg gtttatcaaa
    32461 atcactgctt acgctgacga gctgctcaac gatctggata aactggatca ctggccagac
    32521 accgttaaaa ccatgcagcg taactggatc ggtcgttccg aaggcgtgga gatcaccttc
    32581 aacgttaacg actatgacaa cacgctgacc gtttacacta cccgcccgga cacctttatg
    32641 ggttgtacct acctggcggt agctgcgggt catccgctgg cgcagaaagc ggcggaaaat
    32701 aatcctgaac tggcggcctt tattgacgaa tgccgtaaca ccaaagttgc cgaagctgaa
    32761 atggcgacga tggagaaaaa aggcgtcgat actggcttta aagctgttca cccattaacg
    32821 ggcgaagaaa ttcccgtttg ggcagcaaac ttcgtattga tggagtacgg cacgggcgca
    32881 gttatggcgg ttccgggtca cgaccagcgc gactacgagt ttgcctctaa atacggcctg
    32941 aacatcaaac cggttatcct ggcggctgac ggctctgagc cagatctttc tcagcaagcc
    33001 ctgactgaaa aaggcgtgct gttcaactct ggcgagttca acggtcttga ccatgaagcg
    33061 gccttcaacg ccatcgccga taaactgact gcgatgggcg ttggcgagcg taaagtgaac
    33121 taccgcctgc gcgactgggg tgtttctcgc cagcgttact ggggcgcgcc gattccgatg
    33181 gtgacgctgg aagacggtac cgtaatgcca accccggacg accagctgcc ggtgatcctg
    33241 ccggaagatg tggtgatgga cggcattacc agcccgatta aagcagatcc ggagtgggca
    33301 aaaactaccg ttaacggtat gccagcgctg cgtgaaaccg acactttcga cacctttatg
    33361 gagtcctcct ggtactatgc gcgctacact tgcccagagt acaaagaagg tatgctggat
    33421 tccgaagcgg ctaactactg gctgccggtg gatatctaca ttggtggtat cgaacacgcc
    33481 attatgcacc tgctctactt ccgcttcttc cacaaactga tgcgtgatgc aggcatggtg
    33541 aactctgacg aaccagcgaa acagttgctg tgtcagggta tggtgctggc agatgccttc
    33601 tactatgttg gcgaaaacgg cgaacgtaac tgggtttccc cggttgatgc tatcgttgaa
    33661 cgtgacgaga aaggccgtat cgtgaaagcg aaagatgcgg caggccatga actggtttat
    33721 accggcatga gcaaaatgtc caagtcgaag aacaacggta tcgacccgca ggtgatggtt
    33781 gaacgttacg gcgcggacac cgttcgtctg tttatgatgt ttgcttctcc ggctgatatg
    33841 actctcgaat ggcaggaatc cggtgtggaa ggggctaacc gcttcctgaa acgtgtctgg
    33901 aaactggtgt acgagcacac agcaaaaggt gatgttgcgg cactgaacgt tgatgcgctg
    33961 actgaagatc agaaagcgct gcgtcgcgat gtgcataaaa ctatcgctaa agtgaccgat
    34021 gatatcggcc gtcgtcagac cttcaacacc gcaattgcgg cgattatgga gctgatgaac
    34081 aaactggcga aagcaccgac cgatggcgag caggatcgcg ctctgatgca ggaagcactg
    34141 caggccgttg tccgtatgct caacccgttc accccgcaca tctgcttcac gctgtggcag
    34201 gaactcaaag gcgaaggcga tatcgacaac gcgccgtggc cggttgctga cgaaaaagcg
    34261 atggtggaag actccacgct ggtcgtggtg caggttaacg gtaaagtccg tgccaaaatc
    34321 accgtcccgg tggacgcaac ggaagaacag gttcgcgaac gtgctggcca ggaacatctg
    34381 gtagcaaaat atcttgatgg cgttactgta cgtaaagtga tttacgtacc aggtaaactc
    34441 ctcaatctgg tcgttggcta agcgcgggag gaagcgtgcg atatctggca acactgttgt
    34501 tatctctggc ggtgttaatc accgccgggt gtggctggca tctgcgtgac accacgcagg
    34561 ttccttccac tatgaaggtc atgatcctgg actcaggcga tccgaacggg ccattaagcc
    34621 gtgcggtgcg taaccagtta cgtctgaatg gtgtcgagtt gcttgataaa gaaaccacgc
    34681 gtaaggacgt tccatccctg cgtttgggta aagtgagcat cgcgaaagat accgcatcgg
    34741 tattccgtaa cggtcaaaca gcagagtatc agatgatcat gacggtgaat gcgaccgtgt
    34801 tgatccccgg ccgtgatatc tacccgatta gcgccaaagt cttccgttca ttcttcgata
    34861 acccgcaaat ggcgttagcg aaagataacg aacaagacat gatcgtaaaa gagatgtacg
    34921 accgtgctgc cgaacagctg attcgtaagc tgccaagcat ccgtgctgcg gatattcgtt
    34981 ccgacgaaga acagacgtcg accacaacgg atactccggc aacgcctgca cgcgtctcca
    35041 ccatgctggg taactgatga ttcggttgta cccggaacaa ctccgcgcgc agctcaatga
    35101 agggctgcgc gcggcgtatc ttttacttgg taacgatcct ctgttattgc aggaaagcca
    35161 ggacgctgtt cgccaggtag ctgcggcaca aggattcgaa gaacaccaca ctttttccat
    35221 tgatcccaac actgactgga atgcgatctt ttcgttatgc caggctatga gtctgtttgc
    35281 cagtcgacaa acgctattgc tgttgttacc agaaaacgga ccgaatgcgg cgatcaatga
    35341 acaacttctc acactcaccg gacttctgca tgacgacctg ctattgatcg tccgcggtaa
    35401 taaattaagc aaagcgcaag aaaatgccgc ctggtttact gctcttgcga atcgcagcgt
    35461 gcaggtgacc tgtcagacac cggagcaggc tcagcttccc cgctgggttg ctgtgcgcgc
    35521 aaaacagctc aacttagaac tggatgacgc ggcgaatcag gtgctctgct actgttatga
    35581 aggtaacctg ctggcgctgg ctcaggcact ggagcgttta tcgctgctct ggccagacgg
    35641 caaactgaca ttaccgcgcg ttgaacaggc ggtgaatgat gccgcgcatt tcaccccttt
    35701 tcattgggtt gatgctttgt tgatgggaaa aagtaagcgc gcgttgcata ttcttcagca
    35761 actgcgtctg gaaggcagcg agccggttat tttgttgcgc acattacaac gtgaactgtt
    35821 gttactggtt aacctgaaac gccagtctgc ccatacgcca ctgcgtgcgt tgtttgataa
    35881 gcatcgggta tggcagaacc gccggggcat gatgggcgag gcgttaaatc gcttaagtca
    35941 gccgcagtta cgtcaggccg tgcaactcct gacacgaacg gaactcaccc tcaaacaaga
    36001 ttacggtcag tcagtgtggg cagagctgga agggttatct cttctgttgt gccataaacc
    36061 cctggcggac gtatttatcg acggttgata tgaaatcttt acaggctctg tttggcggca
    36121 cctttgatcc ggtgcactat ggtcatctaa aacccgtgga aacgctggcg aatttgattg
    36181 gtctgacgcg ggtcacaatc atccctaata atgttcctcc gcatcgtccc cagccggaag
    36241 cgaacagcgt gcagcgtaaa cacatgcttg aactggcgat tgccgacaag ccattattta
    36301 ctcttgatga acgcgagcta aagcgcaatg ccccctctta cactgcgcaa acactgaaag
    36361 agtggcggca ggaacaagga ccggacgtgc cgctggcgtt tattattggt caggattcat
    36421 tgctgacctt tccgacctgg tacgaatacg aaacgatact cgacaatgca catttgatcg
    36481 tctgtcggcg tccaggttac ccacttgaaa tggcgcaacc gcaataccag caatggctgg
    36541 aagatcattt gacacataac ccggaagatc ttcaccttca gcctgccggt aaaatttatc
    36601 tggctgaaac gccgtggttt aacatctcgg cgaccatcat ccgcgaacgt ttgcaaaacg
    36661 gtgaatcgtg tgaggattta ttgccggaac cggtattgac ttacattaac caacaaggct
    36721 tgtatcgctg atacctgtcg ttacggaggg catgatgcga ctgtggttaa ttcgtcatgg
    36781 cgaaacacaa gcgaatgtcg atggtctgta cagcggtcat gcgcccacac ccctgaccgc
    36841 acgcggtatt gagcaagcgc aaaatctgca tacactgcta gatgatgttt cctttgactt
    36901 ggttttatgc agtgaactgg aacgggcaca gcataccgcg cgactggttc tcagtgaccg
    36961 ccagctcccc gttcatatca tccctgaact caacgaaatg ttttttggcg actgggagat
    37021 gcgacatcat cgcgacctca tgcaagaaga tgccgaaaac tatagcgcgt ggtgcaatga
    37081 ctggcagcat gctatcccca cgaacggtga aggatttcag gcattttcgc aacgtgtgga
    37141 acgctttatc gcaaggctta gtgaatatca gcactatcag aatattttaa tcgtcagcca
    37201 tcagggcgta ctgagtctgt taattgctcg tttaattggc atgcctgccg agtctatgtg
    37261 gcattttcgc gttgaccagg ggtgttggag cgccattgat atcaaccaaa aattcgcgac
    37321 gctacgcgtc ctcaatagcc gtgccatcgg ggtcgagaac gcatgacttt tctgtttttt
    37381 tacgggtaag ccgcaacgac cattgacagc cccgggcagg ctgatattct ccgcagccag
    37441 actttttccg ccagacacga ctttgtagaa attgttttac aaaaatggcg atgcaatctg
    37501 cggcgcgggg tgggatgata gcccactttc gaaagccgat tcggcgacaa ttgtcccgaa
    37561 atcgcctctg gttcaggtat actgacagac catttttatc tatttgattc acccaggggg
    37621 aaaacttgca gggtaaagca ctccaggatt ttgttatcga caaaattgat gacctcaaag
    37681 gtcaggacat catcgcctta gacgttcaag gcaaatccag catcaccgac tgcatgatca
    37741 tctgtacggg tacgtccagc cgtcatgtta tgtccattgc tgaccacgtt gtgcaggagt
    37801 ctcgcgcagc gggcctgtta ccgctcggcg tagaaggtga aaacagcgcc gactggattg
    37861 tcgtggattt gggcgatgtg attgtccatg tcatgcagga agagagccgt cgcctgtatg
    37921 aactggaaaa actctggagt taatgcgtga agctgcaact tgtcgccgtg ggaacgaaaa
    37981 tgccggactg ggtacaaacc ggttttaccg agtacctgcg tcgttttccg aaagatatgc
    38041 ccttcgagct gattgaaatt ccggccggca aacgcggcaa gaatgcggac atcaagcgca
    38101 tactcgacaa agagggtgag cagatgttgg cggccgcagg caaaaaccgc attgtcaccc
    38161 tcgatattcc aggcaagccc tgggatacgc cgcagttagc cgctgagctg gaacgctgga
    38221 agctggatgg tcgcgacgta agtctactga ttggcgggcc tgaagggttg tcgcctgcct
    38281 gtaaagcggc ggctgagcag agctggtcgc tgtcggcgct taccctcccc catccgctgg
    38341 ttcgcgtgct ggtcgcagag agtctgtacc gggcgtggag catcaccacc aaccatcctt
    38401 atcaccgtga gtgataaggg agctttgagt agaaaacgca gcggatgaaa ctacagaact
    38461 cttttcgcga ctatacggca gagtccgcgc tgtttgtgcg ccgggcgctg gtcgcctttt
    38521 tggggatttt gctgctgacc ggcgtgctta tcgccaacct gtataatctg caaattgttc
    38581 gctttaccga ctaccagacc cgctctaatg aaaaccgcat taagctggtg cctatcgcgc
    38641 ccagccgcgg cattatctac gaccgtaacg gtatccctct ggccctcaac cgcactatct
    38701 accagataga aatgatgccg gagaaagtcg ataacgtgca gcaaacgctg gacgctttgc
    38761 gcagcgtggt agatctgacc gatgacgata ttgctgcatt ccgaaaagag cgcgcacgtt
    38821 cacaccgttt cacctctatt ccggtgaaaa ctaacctaac cgaagtacaa gtagctcgct
    38881 ttgccgtcaa tcagtaccgt tttccgggtg tcgaagttaa aggctataaa cgtcgttact
    38941 atccttacgg ttcggcgttg acccacgtca tcggctatgt gtcgaaaatc aacgataaag
    39001 acgtcgaacg cctgaataat gacggcaaac tggccaacta tgcggcaacg catgatattg
    39061 gtaagctggg cattgagcgt tactatgaag acgtgctgca cggtcagacc ggttatgaag
    39121 aggttgaagt taacaaccgt gggcgtgtta tccgccagtt aaaagaagta ccaccgcaag
    39181 ccggacacga tatttacctg acgctggatc tcaaactcca gcaatatatt gaaacgctgc
    39241 tggcgggtag ccgcgcagct gtggttgtta ccgaaccgcg tacaggtggg gtgctggcgc
    39301 tggtttccac gcctagttat gacccaaacc tgtttgttga cggtatctcc agcaaagatt
    39361 attccgcctt gctgaacgac ccgaatacac cgctggtgaa ccgcgccaca cagggggttt
    39421 atcctcccgc gtctacagtt aaaccctatg tggcggtttc ggcattaagc gccggggtga
    39481 tcacgcgcaa tacgacgctg tttgacccag gctggtggca actaccaggg tcggaaaaac
    39541 gttatcgtga ctggaaaaaa tggggccacg ggcgtctgaa tgtcacaaga tcgctggaag
    39601 aatctgcgga taccttcttc tatcaggtgg cctacgatat ggggatcgat cgcctctccg
    39661 aatggatggg taaattcggt tatggtcatt acaccggtat cgacctggcg gaagaacgtt
    39721 ccggcaacat gcctacccgc gaatggaaac agaaacgctt taaaaaaccg tggtatcagg
    39781 gtgacaccat tccggttggt atcggccagg gttactggac agcgacccca atccagatga
    39841 gtaaggcact gatgatcctg attaatgacg gtatcgtgaa ggttccgcat ttgctgatga
    39901 gcaccgccga agacggcaaa caggtgccat gggtacaacc gcatgaaccg cccgtcggcg
    39961 atattcattc cggttactgg gagctggcga aagacggtat gtacggtgtt gctaaccgcc
    40021 ctaatggtac ggcgaataaa tactttgcca gcgcaccgta caaaattgcg gcgaaatctg
    40081 gtaccgctca ggtcttcggt ctgaaagcga acgaaaccta taatgcgcac aaaattgccg
    40141 agcgtttacg tgaccacaaa ctgatgaccg cctttgcgcc atacaacaat ccgcaagtgg
    40201 ctgtcgccat gattctggag aacggcggcg cgggtccggc ggttggtacg ctgatgcgcc
    40261 agatcctcga ccacattatg ctgggtgata acaacaccga tctgcctgcg gaaaatccag
    40321 cggttgccgc agcggaggac cattaatcat gacggataat ccgaataaaa aaacattctg
    40381 ggataaagtc catctcgatc ccacaatgct gctgatttta ctggcattgc tggtttacag
    40441 cgccctggtt atctggagcg ccagcggtca ggatattggc atgatggagc gtaaaatcgg
    40501 ccaaatcgcg atgggtctgg tcatcatggt ggtgatggcg caaattcctc cacgcgttta
    40561 tgaaggctgg gccccctatc tctatatcat ctgtattatt ttgctggtgg cggtagatgc
    40621 tttcggagcc atctctaaag gcgctcaacg ctggctggac ctcggtattg ttcgttttca
    40681 gccgtcggaa attgccaaaa tagccgtacc actgatggtt gcgcgcttta tcaaccgcga
    40741 cgtttgcccg ccatcgttga agaatactgg catcgcgctg gtgctgatat ttatgcccac
    40801 gctgctggtg gctgcacagc ctgacctggg aacatcaatc ctcgttgcgc tttccggtct
    40861 gtttgtactg ttcctctctg gccttagctg gcgtctgatt ggcgtcgcag tagtgctggt
    40921 agcggcgttc attccgattc tgtggttctt cctgatgcat gattaccagc gccagcgcgt
    40981 aatgatgctc ctggacccgg aatcagaccc actcggcgcg ggctatcaca ttattcagtc
    41041 taaaattgct attggctccg gcggattacg cggcaaaggc tggctgcacg gcactcagtc
    41101 acagcttgaa tttctccccg aacgccatac tgactttatc ttcgcggtac tggcggaaga
    41161 gctgggatta gtgggcattc tgattctgct cgctctctac attctgctga tcatgcgcgg
    41221 gctgtggata gccgccagag cgcaaaccac ctttggtcgc gtcatggctg gtggcttaat
    41281 gctgatatta ttcgtttatg tcttcgtaaa tattggtatg gtaagcggta ttctgccggt
    41341 tgtaggggtt ccgctcccac tggtcagtta tggaggatcg gcgctaattg tgctgatggc
    41401 tgggttcggg attgtaatgt caatccacac ccacaggaaa atgttgtcga aaagcgtgta
    41461 agaggtgcgc aatgcgtaag cagtggctcg ggatctgcat cgcggcagga atgctcgcgg
    41521 catgtacaag cgatgatggt cagcaacaga cggtaagtgt accgcagcct gcggtatgta
    41581 acggccccat agttgaaatt agcggggcgg acccgcgttt cgaaccactg aacgcgacgg
    41641 caaatcagga ttaccagcgc gacggtaaaa gctacaaaat cgtgcaggat ccgtctcgat
    41701 ttagccaggc gggactggcg gcaatctatg atgccgaacc aggcagtaac ctgacggcct
    41761 ctggcgaagc tttcgatccg acaaagctga cggcggccca tccaacgctt ccgatcccca
    41821 gctacgccag aatcactaac ctggctaacg ggcgaatgat cgtggtgcgc attaatgatc
    41881 gcggccctta cggcaacgac cgcgttattt cgctttctcg cgcggcagct gaccgtctta
    41941 acacgtcaaa caacaccaaa gttcgtatcg atccgattat tgtcgcccag gatggttcgc
    42001 tttctggtcc tggtatggct tgtaccacag tcgccaaaca gacttacgcc ctgcctgcac
    42061 ctcccgattt aagcggtggc gcgggaacaa gttcagtgtc tggcccgcag ggtgacattc
    42121 ttccggtcag taattcgacg ctaaaaagcg aagatccgac cggcgcgccg gtaaccagta
    42181 gcggtttcct cggcgctcca acgaccttag cgcctggtgt actggaaggc agcgaaccga
    42241 cgcctgcgcc acagcccgtt gttacagctt cgtcgacaac gcctgcaacc tcgcctgcaa
    42301 tggtgacacc gcaagccgcc tcgcaaagcg ccagcggcaa ctttatggtg caagtcgggg
    42361 ccgtaagcga tcaggctcgt gcgcaacagt accaacagca actgggacag aagttcggcg
    42421 tccccggtcg cgtaactcaa aatggcgcgg tctggcggat ccagcttggc ccattcgcca
    42481 gcaaagccga agccagtacc ttgcagcaac gtttgcaaac cgaagcccaa ttacagtcat
    42541 ttattactac cgcgcagtag cgtaaagcag gcatctgatg tgtcaatttc tgtaaatgac
    42601 gttaacaaag tcacgcggaa agtcagatgc ctgccggtag tgcatttgct atagtagggc
    42661 acttttttaa ttccatcacg gatgtcgtag ttcagaccat gaataccatt ttttccgctc
    42721 gtatcatgaa gcgcctggcg ctcaccacgg ctctttgcac agcctttatc tctgctgcac
    42781 atgccgatga cctgaatatc aaaactatga tcccgggtgt accgcagatc gatgcggagt
    42841 cctacatcct gattgactat aactccggca aagtgctcgc cgaacagaac gcagatgtcc
    42901 gccgcgatcc tgccagcctg accaaaatga tgaccagtta cgttatcggc caggcaatga
    42961 aagccggtaa atttaaagaa actgatttag tcactatcgg caacgacgca tgggccaccg
    43021 gtaacccggt gtttaaaggt tcttcgctga tgttcctcaa accgggcatg caggttccgg
    43081 tttctcagct gatccgcggt attaacctgc aatcgggtaa cgatgcttgt gtcgccatgg
    43141 ccgattttgc cgctggtagc caggacgctt ttgttggctt gatgaacagc tacgttaacg
    43201 cactgggcct gaaaaatacc cacttccaga cggtacatgg tctggatgct gatggtcagt
    43261 acagctccgc gcgagatatg gcgctgatcg gccaggcatt gatccgtgac gtaccgaatg
    43321 aatactcgat ctataaagaa aaagaattta cgtttaacgg tattcgccag ctgaaccgta
    43381 acggcctgtt atgggataac agcctgaatg tcgacggcat caaaaccgga cacactgaca
    43441 aagcaggtta caatcttgtt gcttctgcga ctgaaggcca gatgcgcttg atttctgcgg
    43501 tgatgggtgg acgtactttt aaaggccgtg aagccgaaag taaaaaactg ctaacctggg
    43561 gcttccgttt cttcgaaacc gttaacccac tgaaagtagg taaagagttc gcctctgaac
    43621 cggtttggtt tagtgattct gatcgcgctt cgttaggggt tgataaagac gtgtacctga
    43681 ccattccgcg tggtcgcatg aaagatctga aagccagcta tgtgctgaac agcagtgaat
    43741 tgcatgcgcc gctgcaaaag aatcaggtcg tcggaactat caacttccag cttgatggca
    43801 aaacgatcga gcaacgcccg ctggttgtgt tgcaagaaat cccggaaggt aacttcttcg
    43861 gcaaaatcat tgattacatt aaattaatgt tccatcactg gtttggttaa aaattgaaca
    43921 cttgaaagtg taatttccgt ccccatatac taagcatcag taaaaaaact cccgccttct
    43981 ggcgggagtt gctatttaat tacgttacgc cggagctgac atgaaaacca aacttaacga
    44041 actgcttgaa ttccctactc cttttactta caaagttatg gggcaggcgt tacctgagct
    44101 ggttgatcag gtggttgaag tggtacagcg ccatgcgcca ggtgactaca ccccaacggt
    44161 aaaaccaagc agcaaaggca actaccactc ggtatctatc actatcaacg ccactcatat
    44221 cgagcaggtt gaaacactgt atgaagaact gggcaaaatc gatattgtcc gcatggttct
    44281 gtaactcgct tctccgttac ccggctcctg gtcgggtaac tcccctctcc tcgctgtgat
    44341 atacttttcc cccactttta ctcattctcc acggagatgc cgttttgtat caggataaaa
    44401 ttcttgtccg ccagctcggt cttcagcctt acgagccaat ctcccgggct atgcatgaat
    44461 tcaccgatac ccgcgataat agtacccttg atgaaatctg gctggtcgag cactatccgg
    44521 tattcaccca aggtcaggca ggaaaagcgg agcacatttt aatgccgggt gatattccgg
    44581 tgatccagag cgatcgcggt gggcaggtga cttatcacgg gccgggacaa caggtgatgt
    44641 atgtgttgct taacctgaaa cgccgtaaac tcggtgtgcg tgaactggtg accttgcttg
    44701 agcaaacagt ggtgaatacc ctggctgaac tgggtataga agcgcatcct cgggctgacg
    44761 cgcctggtgt ctatgtcggg gaaaagaaaa tttgctcact gggtttacga attcgacgcg
    44821 gttgttcatt ccacggtctg gcattaaacg tcaatatgga tctttcacca tttttacgta
    44881 ttaatccttg tgggtatgcc ggaatggaaa tggctaaaat atcacaatgg aaacccgaag
    44941 cgacgactaa taatattgct ccacgtttac tggaaaatat tttagcgcta ctaaacaatc
    45001 cggacttcga atatattacc gcttaattcc atacatcaat ggcccaattt acactgggtc
    45061 attactcata atacctgcta atgctttaca tctccttcgc gtttgtatat tttctttatc
    45121 acttgcagaa atgcggtgcc acgccttgct ccgtaactca ccccattgat taacctattg
    45181 aaatagttga ttatggtgag caaaagcgtt ccgttccaat tagacataag attattgtaa
    45241 ttttttaaat attatttttc catgaataaa attggagtaa gtgcgtggat agtaataatc
    45301 aaattgaacc ctgtttaagc agaaagtcgt cagaaggtaa accacaaata tttacaacct
    45361 tacgaaatat tgatcttaac cttctgacta tttttgaagc tgtatatgta cataaaggga
    45421 tcgttaatgc agcgaaagtg cttaatctga ccccctcggc aatcagtcag tctattcaga
    45481 aactgcgcgt tatattccct gacccattgt ttattcgcaa aggccagggt gtcactccta
    45541 ccgcatttgc gatgcatcta catgagtata tcagtcaggg ccttgagtcc attcttggcg
    45601 cgctggatat cgaaggaagc tatgataagc aacgaacgat aactattgcg accactccct
    45661 cggtcggagc cctggtcctt cctgtcatct atcgggcgat taaaactcac tatccgcagc
    45721 ttttactgcg caacccgccc atcagcgacg cggaaaacca actcagtcag tttcaaaccg
    45781 atctcatcat cgataacatg ttttgcacca atcgtacggt gcaacatcat gttctgttca
    45841 ccgacaatat ggtgttaatt tgccgcaagg gaaatccact actctcttta gaagatgaca
    45901 gagagactat cgacaacgct gcgcatgtac ttctgttacc ggaagggcaa aatttcagcg
    45961 gtctgcggca gagagttcaa gagatgtttc cggaccggca aatcaatttc accagctaca
    46021 acattttgac aatcgctgca ctggttgcca acagtgacat gttagcgatt attcccagcc
    46081 gtttttataa cctgtttagt cgttgctggc cgctggaaaa actgcctttt ccgtccttaa
    46141 atgaggagca aatagatttc tctatccact acaacaaatt catcctgcgt gatcccatcc
    46201 tgcacggagt aatagatgtt atccgtaatg cattttgaaa aagtattgtt acagccagat
    46261 cacagcgcag aacgataaaa tggcacaatc caacaacaat tttacatttt cgcgaccgct
    46321 ttggctgctt tcaggtccgt ttcaatgata tactgccagt cgttaattca aaaatagttg
    46381 ataattacaa caatctattg aattgaaacg ctttccttcg taattcgcaa ctggaacacg
    46441 tacgctatga gtaaacccat tgtgatggaa cgcggtgtta aataccgcga tgccgataag
    46501 atggccctta tcccggttaa aaacgtggca acagagcgcg aagccctgct gcgcaagccg
    46561 gaatggatga aaatcaagct tccagcggac tctacacgta tccagggcat caaagccgca
    46621 atgcgcaaaa atggcctgca ttctgtctgc gaggaagcct cctgccctaa cctggcggaa
    46681 tgcttcaacc acggcacagc aacgtttatg atcctcggcg ctatttgtac ccgccgttgt
    46741 ccgttctgtg atgttgccca cggtcgcccg gtagctcctg atgccaatga accagtgaaa
    46801 ctggcgcaga ccattgccga tatggcgctg cgttatgtgg ttatcacctc cgttgaccgt
    46861 gatgacctgc gcgatggcgg tgcccaacac tttgcggatt gtattactgc cattcgggaa
    46921 aaaagcccgc aaatcaaaat tgaaactctg gtgccggatt tccgcggtcg aatggatcgt
    46981 gctctggata ttctgactgc aacgccccca gatgtgttca accataacct ggaaaacgta
    47041 ccgcgtattt accggcaggt acggcctggc gcagattaca actggtcgct gaagctgctg
    47101 gaacgcttta aagaagcgca tccggaaatc ccgaccaagt ctggtctgat ggtgggtctg
    47161 ggtgaaacca atgaagaaat tattgaggta atgcgcgacc tgcgccgtca tggtgtgacg
    47221 atgttaacgc tggggcaata tttgcagcca agccgccatc acctgccggt tcaacgttac
    47281 gttagcccgg atgagttcga cgaaatgaaa gccgaagcgc tggcgatggg ctttacccat
    47341 gctgcatgcg gtccgtttgt ccgctcttct taccacgccg atttgcaggc gaaaggaatg
    47401 gaagttaagt aatcctgtaa taattactta catctgtctg ataaaaaacc cgctttttga
    47461 agcgggtttt ttgtatcgaa caagatattg agggagcgtc ctgctcgcca cgtcactctt
    47521 tatgagagag cttttcagcc tgaagatcaa cgtctgcgcc ttttttcgcc gcagcatcgt
    47581 catcattcat cgccttcttg aaccctttaa tggccgctcc aaggtctccg cccagcgtac
    47641 gtaacttctt agtcccaaac agcagaacga ccagcgccgc aactaccagc agtttggtaa
    47701 tactaatctc acccatagat accttcttga catataaccg gcaacagaac gcgccttaaa
    47761 aaccagagaa tattaacggt cattcgacgc gcgcacacaa tagcaatctg taacaaggtg
    47821 aatcaagcgc cgagtaaaaa aacatcataa taattgcggc ggcgcaaagc gacggttgtt
    47881 taagatgggc agttgcgcgc gcacctgacg cacacgttcg ggcgtcactt ccgccataat
    47941 gagtgcaggc atttctgacg ctgccgcaat ggtgacgcca aagggatcta taatccggct
    48001 ttgcccgata tttttgttcc cgcactcccc cgccgccacc atataacagg tggtatccag
    48061 cgcacgagcg gcaagcaacg ttgaccagtg atgctctttg agcggcccac gaacccaggc
    48121 ggcaggaagt accaggattt cagctccctg taaagcctgt gccagcgcca gctctggaaa
    48181 gcgtaagtca taacaggtca tcagaccgac cttcatccct tccacctcca gtaacggagc
    48241 tatttcatta ccagcatcaa catggcgtga ttcctgaatg gcaaatgcat catagagatg
    48301 cagtttggca taacgggcga cgatgtttcc tgcctgaagt gccaccagca tattccatgc
    48361 gcgccccggc gttgaaggaa gatgaatcgt cagaattgtc gtcatcatgt tacgtttact
    48421 ttctcgccgt aaacgtccga ggaattcgcc ttccagcagc tgtgctgatt taaccgatag
    48481 atctgcatca tgatcatcgc gcgccagcaa tgcttccggc aggacaaaca gcgatacgtc
    48541 gttttccgcc gcctgcgcca tcaacgaggc acaaatctca gcgttctttt cccacacaga
    48601 tgtaacagca aactgtcctg cagcaaccag cattgccacc tcctgtcaga gtcgtaaaat
    48661 tcgccaacga cgattattcg gcgttacact tatcactcat acaaatcaaa tagcaggatt
    48721 ttgcagtgtt acaacttctt ttagcagttt ttattggcgg tggtacggga agcgtagcga
    48781 gatggctgtt aagtatgcga tttaacccgc tgcatcaggc gattccgttg gggacgctgg
    48841 cagcaaacct gattggggca ttcatcatag gaatgggatt cgcctggttc agcaggatga
    48901 cgaacattga tccagtgtgg aaagtattaa tcaccaccgg attttgtggc ggtctaacaa
    48961 ccttctcaac attttcggca gaagtggtgt ttttgttaca agagggccgc tttggctggg
    49021 cattactgaa cgttttcgtc aaccttctgg ggtcttttgc catgaccgca ctggcattct
    49081 ggctgttttc ggcctcaacc gtacactaaa gaccaaaaaa aacccgctga ttaagcgggt
    49141 tttgaattct tgctgacgta tcttacagag cgattacgtt tgcagcagaa gggcctttgg
    49201 caccgttagt gatttcgaac tctacgcgct gaccttcagc aagagtttta aaaccattag
    49261 tctggattgc agagaagtgt acgaacacgt ctttgctgcc gtcttccgga gtaatgaaac
    49321 cgaatccttt ggactcatta aaccacttaa cgttaccttt aatcttagac atcaaaatta
    49381 cctttacatg aaaaatagac acaaatgctg tgtcggttat cagtacacca attgtggtac
    49441 gctttgtcca gtgcaacttt actaaaaagt gataaaagtc gcgttatttt tgatgttgct
    49501 tattttagtt attgatttta aagaagttac taaaacttca tttgtctcaa aactgaaagc
    49561 gcatccaggc aaagtacaca ttgccattgt tgtaggtacc cggaatgtag gtcatctgaa
    49621 aagtcactgg gccataaccc acggaggcca atggcagtag aaccgggaga gggatgtaat
    49681 tccagttatc gcgtgccgtt acgccagcgg tgaatcccag acctaaatga aaattttcat
    49741 ccgccagcgg tcgccaggta ctttcccatc cgtatccggc aatcggttcc catttgttcc
    49801 acgagtcctt aaatgccatg gcatacaggc catgccagtt tcctttttca tcccaacgcg
    49861 acaggccaaa accgccaccc cacggtcgct cgttatagcg atcggttttt tctttgtcgt
    49921 aagcgaaacg tgcatgccag gtgatggcag gaatatataa atcataatgt tcaggctgtt
    49981 gccaggtttg tgcaatattt tctctaaacg ttgtcatcct ctcatctgcg ttagcaaaaa
    50041 ctttaccaac gctgattaac tgaataaaaa caaaggaaaa gatagcgaca tatttactca
    50101 cgttcatttg tgaccataaa acatttatca aaaatctact actagcatag caaagctaca
    50161 attaacataa ccttaataga cccaacataa agaataatct gaatagctgc tctatttatt
    50221 tccgcttgtt caagttaaat atttccgcgc atcgattaaa gatgatgcgt cgttttctgc
    50281 gatgggaata gtcaaaaaag aaaaaccaag tcttttttga tgacaaaatg caatcaagga
    50341 aaaattattt tattttttta acgagataca acaatcatct taacgaagta tataatatat
    50401 atcgtcaacc catgctcata ttagattatt ttttgagcgc acagccgcag ctaacaccat
    50461 tgcaattaac aaatttgcat caatccacca tcaatttaca cacattatta tgtgataatt
    50521 gccaaccgct aaatatgcgt tttgttatct atgtataaaa acagcaactt caatgtctta
    50581 atggcagttt ttcttgattt taatcagcat tcatcgccaa tttattgggc atattttttc
    50641 cttaagcttt aggaagtttt tattatttac tttgggcctg gagacaggaa aaattatgct
    50701 gacattcatt gaactcctta ttggggttgt ggttattgtg ggtgtggctc gctacatcat
    50761 taaagggtat tccgccactg gtgtgttatt tgtcggtggc ctgttattgc tgattatcag
    50821 tgccattatg gggcacaaag tgttaccgtc cagccaggct tcaacaggct acagcgccac
    50881 ggatatcgtt gaatacgtta aaatattact aatgagccgc ggcggcgacc tcggcatgat
    50941 gattatgatg ctgtgtggct ttgccgctta catgacccat atcggcgcga atgatatggt
    51001 ggtcaagctg gcgtcaaaac cattgcagta tattaactcc ccttacctgc tgatgattgc
    51061 cgcctatttt gttgcttgtc tgatgtcact ggccgtctct tccgcaaccg gtctgggggt
    51121 tttgctgatg gcaaccctgt ttccggtgat ggtaaacgtt ggtatcagtc gtggcgcagc
    51181 agctgccatt tgtgcctccc cggcggcgat tattctcgca ccgacttcag gggatgtggt
    51241 gctggcggcg caggcttccg aaatgtcgct gattgacttc gccttcaaaa cgacgctgcc
    51301 tatctcaatt gctgcaatta tcggcatggc gatcgcccac ttcttctggc aacgttatct
    51361 ggataaaaaa gagcacatct ctcatgaaat gttagatgtc agtgaaatca ccaccactgc
    51421 ccccgcgttt tatgccattt tgccgttcac gccgatcatc ggtgtactga tttttgacgg
    51481 taaatggggt ccgcaattac acatcatcac tattctggtg atttgtatgc tgattgcctc
    51541 cattctggag ttcctccgca gctttaatac ccagaaagtt ttctctggtc tggaagtggc
    51601 ttatcgcggg atggcagatg cgtttgctaa cgtggtgatg ctgctggttg ccgctggggt
    51661 attcgctcag gggcttagca ccatcggctt tattcaaagt ctgatttcta tcgctacctc
    51721 gtttggttcg gcgagtatca tcctgatgct ggtattggtg attctgacca tgctggcggc
    51781 agtcacgacc ggttcaggca atgcgccgtt ttatgcgttt gttgagatga tcccgaaact
    51841 ggcgcactct tccggcatta acccggcgta tttgactatc ccgatgctgc aggcgtcaaa
    51901 cctgggtcgt accctatcac ccgtttctgg cgtagtcgtt gcggttgccg ggatggcgaa
    51961 aatctcacca tttgaagtcg taaaacgcac ctcggtgccg gtgcttgttg ggctagtgat
    52021 tgttatcgtt gctacagagc tgatggtgcc aggaacggca gcagcggtca caggcaagta
    52081 aatagtaatg ccggaggcaa gtttcctccg gctttatcat gtcacccact gtggtatatg
    52141 cgttgtggtc tgccaacttt gccgtgaaca atttcagcaa taatcagatg gcggctggcg
    52201 caatattcaa gataacgcct ggcagtggtg cggctgatgg tcagtgcctg cgccaccgtt
    52261 tctgccgtat gttgcacacc aggctcttta aacagttttc gcaccgcgtt tagcgtcaag
    52321 gggtcaatgc cggtcggcag ttcgtctttg ggttcaccgc gagcataggc attaaacatc
    52381 tcatcaattt gcttctggct ggcgctatca atactttcca gcatatgttt acgctggcgg
    52441 aaacgggtta gcgtttgccc cagtgtattg attcacttga agtacgaaaa aaccgggaga
    52501 acattggatt attcgggatc tgatgggatt agatttggtg gggcttgcaa gcctgtagta
    52561 caaattttag gcgttaacca atgaaacgcg aaagatagta aaaaattgct tttgtttcat
    52621 tgaaaaaacg aaaaacaaaa acactataaa tcatttcaat catagcttca aaaaacgttc
    52681 aaaaccgata acaaccaggc tgtcaccaaa tgactcatat cacaaatcag ctcatgccgt
    52741 ttaggtatgt tacatgtgtg attatgtgag gtgaagtatg ttttagctgg ctcatggttg
    52801 ttatacggct tttttcgcat cttgtggttc caacaaagga gctacaggct cttcctgttt
    52861 atgaacacca tacattgaca aaatctcttg gcgagtttct ggaggtaatt tatgcagcgc
    52921 caaagctgtt tgtagtgttt ggcgatcaat acccagcgtt gccaataggg tgtcaacgga
    52981 gatggagggg cgttcttgca ttaatccgga aagcccatat ttagccatgt ttagctctat
    53041 ctctcggact tcattttctc ctagtttatt gattaacagg gagaacattt tcaatgtatc
    53101 agcattcatt tcttcgatag caattttttg catagccggt attgccggga tatattcaac
    53161 cccaggcccg cgacgccctt cttgtgaacg cttcggccat ccttctttct ttgctcgttt
    53221 atgcaccccc tgtattgtcc caggcattcc atccagttca gtaatttgtt tgggtgtcat
    53281 ccactggttt atctcatttg cgcttttcat aaactaagtt taccagattt atctatataa
    53341 aaatcaatgg attacaaaat aaaccacatt aactgatata aaccggtttg gtttatttgg
    53401 taatatataa aacagataat caagacgaat tatttgtttt ggtaaccctt tatcatacgc
    53461 aatatggttg gtgtgcgaaa gatgagtaac gattggcatc gggccgacgt gattgcggcc
    53521 ctcaaaaaaa caggaacccc tctggcaaaa ttgtccagac gttcaggtta ttcaaccggg
    53581 acgcttgcaa atgcattagt caggccatgg cctaaaggtg aacgcattat tgctgacgcc
    53641 ttaaacatga aacctgagga aatttggcct agtagatatc taagggatca caatgagaga
    53701 gtggatatca gcaattgaat gcatgggttt atctggttta cctggttcat taatgggggt
    53761 tcacaaaaga gctcagagag aaggatggct caagagaaga agagaaggaa ttagaggggt
    53821 tgcttacgaa taccatatat cttctcttcc taacattgct cgagctgaag ttttgctcca
    53881 acaaggaaaa tttaaaacca gtcagggatg ttttgaaatc gcccgcccca cgctggaagc
    53941 tcatgattat gatcgtgagg cactatggag caaatgggat aaagccagcg attcccagcg
    54001 cagcctggct gaaaaatggt tgccctcaat tcaggccaca gacgaaatgc tgaaccaggg
    54061 gatttcaacg aaaacggctt ttgcgaccgt tgcagggcat taccaggtca gcgcatccac
    54121 tttgcgggac aagtattacc aggtacagaa gtttgcgaag cctgactggg cggctgcact
    54181 tgttgatgga cgtggagcat cccgtcgcaa tgttcacaaa agtgaatttg acgaggatgc
    54241 ctggcagttt ctgattgcag attatctgcg accggaaaaa cccgccttcc gcaaatgtta
    54301 tgagcgtctg gaactggcag cccgcgagca tggctggagt attccctccc gtgccacggc
    54361 ctttcgccgg attcagcaac tggacgaggc aatggttgtt gcctgtcgtg aaggtgaaca
    54421 tgcactgatg catctgatac cggcacagca gcgaactgtg gaacacctgg acgccatgca
    54481 gtggatcaac ggcgacggtt atctgcataa cgtctttgta cgctggttta acggtgatgt
    54541 gatccgtccg aaaacatggt tctggcagga tgtgaaaacc cgaaaaattc tgggctggcg
    54601 ctgcgatgtg agcgagaaca ttgattcaat tcgcctctcg ttcatggatg ttgtgactcg
    54661 ctacagtatc ccggaggatt ttcacatcac cattgataac acccgtggtg ctgcgaataa
    54721 atggctgacg ggaggcgcgc ccaatcgcta ccgctttaag gtaaaagagg acgatccaaa
    54781 aggactgttt ttactgatgg gtgcgaaaat gcactggaca agcgttgttg ccggtaaagg
    54841 ctggggccag gcaaaacctg ttgaacgtgc tttcggtgtt ggtgggcttg aggaatacgt
    54901 tgataagcat ccggcactgg ctggcgcata tacggggcca aatccgcagg caaaacctga
    54961 taactatggc gaccacgctg ttgatgcaga gctgtttctg aaaacctttg ccgaaggtgt
    55021 ggcgatgttc aatgccagaa caggccgtga aacagaaatg tgcgggggca aactctcgtt
    55081 tgatgatgtt ttcgagcgtg aatacgccag aacgattgtg cgtaagccaa ccgaagaaca
    55141 aaaacggatg ctgttactgc ctgccgaggc ggtgaacgtt tcacgcaaag gcgagtttac
    55201 gcttaaagtt ggcggctccc ttaaaggcgc gaaaaacgtt tattacaaca tggcattaat
    55261 gaatgccgga gtgaaaaaag ttgtggtcag atttgatcca cagcagttac acagcacggt
    55321 ttattgag
//
LOCUS       DAZSNO010000009        54502 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZSNO010000009 DAZSNO010000000
VERSION     DAZSNO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54502
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.009"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            44..676
                     /gene="comR"
                     /locus_tag="ACHWN2_000488"
     CDS             44..676
                     /gene="comR"
                     /locus_tag="ACHWN2_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR8930164.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(738..1277)
                     /locus_tag="ACHWN2_000489"
     CDS             complement(738..1277)
                     /locus_tag="ACHWN2_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR8930165.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACHWN2_000490"
     CDS             complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACHWN2_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR8930166.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3216..3758)
                     /gene="ycfP"
                     /locus_tag="ACHWN2_000491"
     CDS             complement(3216..3758)
                     /gene="ycfP"
                     /locus_tag="ACHWN2_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR8930167.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3781..4806)
                     /gene="nagZ"
                     /locus_tag="ACHWN2_000492"
     CDS             complement(3781..4806)
                     /gene="nagZ"
                     /locus_tag="ACHWN2_000492"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR8930168.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(4817..5641)
                     /gene="thiK"
                     /locus_tag="ACHWN2_000493"
     CDS             complement(4817..5641)
                     /gene="thiK"
                     /locus_tag="ACHWN2_000493"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR8930169.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5622..6263)
                     /gene="lpoB"
                     /locus_tag="ACHWN2_000494"
     CDS             complement(5622..6263)
                     /gene="lpoB"
                     /locus_tag="ACHWN2_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR8930170.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6278..6655)
                     /locus_tag="ACHWN2_000495"
     CDS             complement(6278..6655)
                     /locus_tag="ACHWN2_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR8930171.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6658..7017)
                     /gene="hinT"
                     /locus_tag="ACHWN2_000496"
     CDS             complement(6658..7017)
                     /gene="hinT"
                     /locus_tag="ACHWN2_000496"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR8930172.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7356..9541
                     /gene="fhuE"
                     /locus_tag="ACHWN2_000497"
                     /pseudo
     CDS             7356..9541
                     /gene="fhuE"
                     /locus_tag="ACHWN2_000497"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(9601..11034)
                     /gene="ptsG"
                     /locus_tag="ACHWN2_000498"
     CDS             complement(9601..11034)
                     /gene="ptsG"
                     /locus_tag="ACHWN2_000498"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR8930173.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11329..12126)
                     /locus_tag="ACHWN2_000499"
     CDS             complement(11329..12126)
                     /locus_tag="ACHWN2_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8930174.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12137..13141)
                     /gene="holB"
                     /locus_tag="ACHWN2_000500"
     CDS             complement(12137..13141)
                     /gene="holB"
                     /locus_tag="ACHWN2_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR8930175.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13138..13779)
                     /gene="tmk"
                     /locus_tag="ACHWN2_000501"
     CDS             complement(13138..13779)
                     /gene="tmk"
                     /locus_tag="ACHWN2_000501"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR8930176.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13769..14791)
                     /gene="yceG"
                     /locus_tag="ACHWN2_000502"
     CDS             complement(13769..14791)
                     /gene="yceG"
                     /locus_tag="ACHWN2_000502"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR8930177.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14794..15603)
                     /gene="pabC"
                     /locus_tag="ACHWN2_000503"
     CDS             complement(14794..15603)
                     /gene="pabC"
                     /locus_tag="ACHWN2_000503"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR8930178.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(15723..16964)
                     /gene="fabF"
                     /locus_tag="ACHWN2_000504"
     CDS             complement(15723..16964)
                     /gene="fabF"
                     /locus_tag="ACHWN2_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR8930179.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17051..17287)
                     /gene="acpP"
                     /locus_tag="ACHWN2_000505"
     CDS             complement(17051..17287)
                     /gene="acpP"
                     /locus_tag="ACHWN2_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR8930180.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17498..18232)
                     /gene="fabG"
                     /locus_tag="ACHWN2_000506"
     CDS             complement(17498..18232)
                     /gene="fabG"
                     /locus_tag="ACHWN2_000506"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR8930181.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18245..19174)
                     /gene="fabD"
                     /locus_tag="ACHWN2_000507"
     CDS             complement(18245..19174)
                     /gene="fabD"
                     /locus_tag="ACHWN2_000507"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR8930182.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19190..20143)
                     /gene="fabH"
                     /locus_tag="ACHWN2_000508"
     CDS             complement(19190..20143)
                     /gene="fabH"
                     /locus_tag="ACHWN2_000508"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR8930183.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20211..21281)
                     /gene="plsX"
                     /locus_tag="ACHWN2_000509"
     CDS             complement(20211..21281)
                     /gene="plsX"
                     /locus_tag="ACHWN2_000509"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR8930184.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21362..21535)
                     /gene="rpmF"
                     /locus_tag="ACHWN2_000510"
     CDS             complement(21362..21535)
                     /gene="rpmF"
                     /locus_tag="ACHWN2_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR8930185.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21587..22108)
                     /gene="yceD"
                     /locus_tag="ACHWN2_000511"
     CDS             complement(21587..22108)
                     /gene="yceD"
                     /locus_tag="ACHWN2_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR8930186.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22307..22891
                     /gene="yceF"
                     /locus_tag="ACHWN2_000512"
     CDS             22307..22891
                     /gene="yceF"
                     /locus_tag="ACHWN2_000512"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR8930187.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23003..23962)
                     /gene="rluC"
                     /locus_tag="ACHWN2_000513"
     CDS             complement(23003..23962)
                     /gene="rluC"
                     /locus_tag="ACHWN2_000513"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR8930188.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24535..27726
                     /gene="rne"
                     /locus_tag="ACHWN2_000514"
     CDS             24535..27726
                     /gene="rne"
                     /locus_tag="ACHWN2_000514"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR8930189.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27923..28865)
                     /gene="flgL"
                     /locus_tag="ACHWN2_000515"
                     /pseudo
     CDS             complement(27923..28865)
                     /gene="flgL"
                     /locus_tag="ACHWN2_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(28877..30519)
                     /gene="flgK"
                     /locus_tag="ACHWN2_000516"
                     /pseudo
     CDS             complement(28877..30519)
                     /gene="flgK"
                     /locus_tag="ACHWN2_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(30585..31526)
                     /gene="flgJ"
                     /locus_tag="ACHWN2_000517"
     CDS             complement(30585..31526)
                     /gene="flgJ"
                     /locus_tag="ACHWN2_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR8930190.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31526..32623)
                     /gene="flgI"
                     /locus_tag="ACHWN2_000518"
     CDS             complement(31526..32623)
                     /gene="flgI"
                     /locus_tag="ACHWN2_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR8930191.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32635..33333)
                     /gene="flgH"
                     /locus_tag="ACHWN2_000519"
     CDS             complement(32635..33333)
                     /gene="flgH"
                     /locus_tag="ACHWN2_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR8930192.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33386..34168)
                     /gene="flgG"
                     /locus_tag="ACHWN2_000520"
     CDS             complement(33386..34168)
                     /gene="flgG"
                     /locus_tag="ACHWN2_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFR8930193.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34306..34893)
                     /gene="flgF"
                     /locus_tag="ACHWN2_000521"
                     /pseudo
     CDS             complement(34306..34893)
                     /gene="flgF"
                     /locus_tag="ACHWN2_000521"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(34995..35057)
                     /locus_tag="ACHWN2_000522"
                     /pseudo
     CDS             complement(34995..35057)
                     /locus_tag="ACHWN2_000522"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35077..36285)
                     /gene="flgE"
                     /locus_tag="ACHWN2_000523"
     CDS             complement(35077..36285)
                     /gene="flgE"
                     /locus_tag="ACHWN2_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR8930194.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(36310..37005)
                     /gene="flgD"
                     /locus_tag="ACHWN2_000524"
     CDS             complement(36310..37005)
                     /gene="flgD"
                     /locus_tag="ACHWN2_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR8930195.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37017..37420)
                     /gene="flgC"
                     /locus_tag="ACHWN2_000525"
                     /pseudo
     CDS             complement(37017..37420)
                     /gene="flgC"
                     /locus_tag="ACHWN2_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(37424..37840)
                     /gene="flgB"
                     /locus_tag="ACHWN2_000526"
     CDS             complement(37424..37840)
                     /gene="flgB"
                     /locus_tag="ACHWN2_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR8930196.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37995..38654
                     /gene="flgA"
                     /locus_tag="ACHWN2_000527"
     CDS             37995..38654
                     /gene="flgA"
                     /locus_tag="ACHWN2_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR8930197.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            38730..39023
                     /gene="flgM"
                     /locus_tag="ACHWN2_000528"
     CDS             38730..39023
                     /gene="flgM"
                     /locus_tag="ACHWN2_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR8930198.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39028..39444
                     /gene="flgN"
                     /locus_tag="ACHWN2_000529"
     CDS             39028..39444
                     /gene="flgN"
                     /locus_tag="ACHWN2_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR8930199.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(39484..41019)
                     /gene="murJ"
                     /locus_tag="ACHWN2_000530"
     CDS             complement(39484..41019)
                     /gene="murJ"
                     /locus_tag="ACHWN2_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR8930200.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41186..42052)
                     /locus_tag="ACHWN2_000531"
                     /pseudo
     CDS             complement(41186..42052)
                     /locus_tag="ACHWN2_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(42054..42701)
                     /locus_tag="ACHWN2_000532"
     CDS             complement(42054..42701)
                     /locus_tag="ACHWN2_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HFR8930201.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42712..43296)
                     /gene="rimJ"
                     /locus_tag="ACHWN2_000533"
     CDS             complement(42712..43296)
                     /gene="rimJ"
                     /locus_tag="ACHWN2_000533"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR8930202.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43532..44740
                     /gene="mdtH"
                     /locus_tag="ACHWN2_000534"
     CDS             43532..44740
                     /gene="mdtH"
                     /locus_tag="ACHWN2_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR8930203.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44804..45451
                     /gene="grxB"
                     /locus_tag="ACHWN2_000535"
     CDS             44804..45451
                     /gene="grxB"
                     /locus_tag="ACHWN2_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR8930204.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            45585..46145
                     /locus_tag="ACHWN2_000536"
     CDS             45585..46145
                     /locus_tag="ACHWN2_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8930205.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46251..47297
                     /gene="pyrC"
                     /locus_tag="ACHWN2_000537"
     CDS             46251..47297
                     /gene="pyrC"
                     /locus_tag="ACHWN2_000537"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR8930206.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47371..47616
                     /gene="dinI"
                     /locus_tag="ACHWN2_000538"
     CDS             47371..47616
                     /gene="dinI"
                     /locus_tag="ACHWN2_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR8930207.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47906..48160
                     /gene="bssS"
                     /locus_tag="ACHWN2_000539"
     CDS             47906..48160
                     /gene="bssS"
                     /locus_tag="ACHWN2_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR8930208.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48275..49393
                     /gene="solA"
                     /locus_tag="ACHWN2_000540"
     CDS             48275..49393
                     /gene="solA"
                     /locus_tag="ACHWN2_000540"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR8930209.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            49441..49554
                     /locus_tag="ACHWN2_000541"
     CDS             49441..49554
                     /locus_tag="ACHWN2_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFR8930210.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49815..50381
                     /locus_tag="ACHWN2_000542"
     CDS             49815..50381
                     /locus_tag="ACHWN2_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR8930211.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50385..50960
                     /locus_tag="ACHWN2_000543"
     CDS             50385..50960
                     /locus_tag="ACHWN2_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR8930212.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(51002..52054)
                     /locus_tag="ACHWN2_000544"
     CDS             complement(51002..52054)
                     /locus_tag="ACHWN2_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR8930213.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            52279..53199
                     /gene="lpxL"
                     /locus_tag="ACHWN2_000545"
     CDS             52279..53199
                     /gene="lpxL"
                     /locus_tag="ACHWN2_000545"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR8930214.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53371..53556
                     /locus_tag="ACHWN2_000546"
                     /pseudo
     CDS             53371..53556
                     /locus_tag="ACHWN2_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            53592..>54502
                     /locus_tag="ACHWN2_000547"
     CDS             53592..>54502
                     /locus_tag="ACHWN2_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR8930215.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT        13317 a        14507 c        13442 g        13236 t
ORIGIN      
        1 ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac
       61 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
      121 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
      181 cgaagcaacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
      241 atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
      301 gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa
      361 ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct
      421 ctccccagat tcaggagata tagccaatac gttgaaatca cgccatgcga tgcaagagcg
      481 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
      541 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
      601 cgaaggtgca tcgctggaaa aactgatgca gattgccgga acgactttgc gtttatggcc
      661 cgaactggtg aaataagtcg caaaaaaggc ccctcagcct gagcgaatga ggggccaaat
      721 tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccgtt actatctagc
      781 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
      841 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
      901 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
      961 ccaccaccca gcgcccccac aacagtggcg acatctttac cgcgaccacc accaaactga
     1021 tgccctatca cgccaccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc
     1081 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
     1141 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccccg ttcaaacacg
     1201 ttcagactgg ccactgccgc tacgcccaac gcagctgcga caccaatccc gatacccgcc
     1261 aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga
     1321 aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatgactgg aaatacttaa
     1381 ggattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
     1441 ccgctcgtac catttgtcca gggcggataa ggcgtttata ccacatccgc cagtgtacgt
     1501 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
     1561 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata
     1621 tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
     1681 gctaccaacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata
     1741 atttttcagc ggcttaccgt tcatctgcgc cagaatgttg ttcattgcgc aagtcgccat
     1801 ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc
     1861 gcagtcgcca atagcgtaaa tgtctggatc gcgagtggtt tgcagcgtcg gttccaccac
     1921 cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt
     1981 gatcccggct gcccatacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag
     2041 gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt
     2101 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc
     2161 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
     2221 cagttgcttg atcgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc
     2281 gacaatcgca atgttcactt tgccatttgc gcccaggttg gcagagtatt tcaggaacag
     2341 attcagcatc tcctggtgga agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
     2401 ctctttgacg cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca tgaccagggt
     2461 gtcataggcg attttacgtt ccggaaccaa cagttcacct ttctcgtcgc gtagttctgc
     2521 aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccaact ggaactggaa
     2581 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
     2641 agtcgccact tcgtgcagca gtggtttcca caggtggctg tggttacgat cgaccagcgt
     2701 aattttggct tttttcttac gtcccagctt atgccccagc tgtgttgcca tttccagccc
     2761 accagcaccg ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg tgcccccctt
     2821 aaatttatta accaattgtt aattaatggt tattaacata gctcttaatt aacaacaggt
     2881 tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat tggtcatacc
     2941 aaaattgatg ttaatcaagt tttgttgctt aaaagatagg caactttacc gggggagatc
     3001 aaaaaataga gtttattgtt tattatttga gcaaattcgg aagggtaata tagggtgaag
     3061 cgtatatcga gggcgtttgg tcgtcctgat aagatgcgca gcatcgcatc aggcattgat
     3121 gctcgactgt cggatgcggc gtggacgcct tatccgactt acagaattcg gagcgcatca
     3181 ggtaccggca aggttacctg atgcgacgag ggcatttacc cgagggtttt gaacgcttta
     3241 atgcgctgta aatgcgggga gatattcttg aatttgtgcg tctgctcttc gtcccagaca
     3301 atctcgtaat aatgatgcaa ctcttcagat gtccgctggc tgttaagtgc ttcatcatta
     3361 cgcgacaaaa taaccagaca acgatcgcga ttcttctcgc ggaagttggt cacacactta
     3421 gtggcaatat cggcatactc ttccgggcga tcaatcttgc cttccatgtt ctcataaggg
     3481 aacaaattag ggttgaagat cacctggcgg atatcgcaaa gaaaaccaat ccgttccgcc
     3541 cagtatccgc ccaggccaac gccgcaaatt agcggacgct cgtcaacgtt cagttgcaac
     3601 attttgtcca cttctttaag cagatgctgc atatcatgtt tcggatgccg cgtactgtag
     3661 cttatcaagc gtacatccgg gtcaataaac tgcaattgta agactttctc gtggttaccc
     3721 ggactgttag agtcaaaacc gtgtaaatag ataatcatcg cttcctcaca taagccaggg
     3781 ttagtgacct actttctctt cctgccagcg ttcatgtaac tgattcagac gggtgctgat
     3841 cgctttccag cgagccgagt ccatcagttc ctgtcgcgaa aatgaacctt tatgatacaa
     3901 acgtgtaaca cgttctgcct tgatcggtga cagattatct aacacgctga cggccccttt
     3961 acgattattg cagaccagga tcatatcgca acccgcatcc agtgatgcct gcccgcgttc
     4021 ggcataactg cccataatcg cggcaccttc catcgataaa tcgtcagaga aaatcacgcc
     4081 gtcaaacccc agttcctgac gcaaaacggt tttcagccag tagggagaac cgctcgccgg
     4141 acgcggatca acatcactgt agatcacatg cgcaggcata atggcgtcga gtttattttc
     4201 gcggattaag gaactgaaga ccgacatatc tttagcgcga atctccgctt gtggacgtgg
     4261 atcgcacggt gtttctttgt gtgagtctgc cgttactgca ccgtgtcctg ggaagtgttt
     4321 cccggtcgtt ttcattccgg cttcatgcat accatcaata aaccggctgg caattgccag
     4381 ggctttttgt ggatcggcat gataagaacg ctcgccaatc gccgcgctga tatgcccgac
     4441 atccagcact ggcgcaaagc tgatatcaat atccatagcg atcatttcgc tggccatcag
     4501 ccaacccgcc tcttgcgcca gtttgccgcc ctcttccatt cccaacagcg cagcaaagga
     4561 ttgtgctgcc ggtaagcggg taaaaccttc gcgaaaacgc tgcacgcgtc caccttcctg
     4621 atctaccgcc accaccagat gattgcgcga tgctgcgcgg atctggcgca ccagttcacg
     4681 taactgggca ggatcatgat agttacgcgt aaagagaatc agccctccca ccagcggatg
     4741 cgccagtatt tcacgctctt ccgcgtccag ttcgtaacct tcgacatcca acattactgg
     4801 acccacactg ctctccttat tgttttatta atagctgccg ccaggtgtca tcggccagcc
     4861 tgataaattg ttgatcgccg gtttgtcgcc agcggtactc aaaccaccct cctttgagca
     4921 tcagcagcca gggaaaccat cgcctgacct gacgccataa ttgcgccgga taaatcttcg
     4981 cgcgagtggc atagtcattg accaattgcc ggtgctgttc agtattttcc acccacaccg
     5041 ccgccagttc cagcgcgata tcaccatctc cagcatactc ccagtcgatg agttttaacc
     5101 ctgatgcgct atgcactaaa tttccggcgt ggacatccat atgcaatgga cttaagcgta
     5161 aaggtcgtgg ttcccgcgct ttgcgcagac gttttaacat tcgcagccaa cccacagtcc
     5221 gccgcgccgg atcgctttgc tgccagtaca gttccagtaa gggcaacagc gttattcgcc
     5281 agccaaaacg tggttgttga tgtagataat acagcaagcc tgccagttcg ttggtatccg
     5341 gcaaatacgt ttttaccgca ccgggcagat agtcgactac catccagtca cggagatata
     5401 aatgcggctt cggtgcaatg cttgcgggta gttgtgataa agcccgatac tggcgcaaga
     5461 acgcggactg cggcgcatca ggatcgtgcg gctgacgcac aacaaaacgc tggccctgat
     5521 gttcaatgag aaaactaccg ccactaagcc cactattaaa cgtcgtgacg ggatgaaact
     5581 gcgggaaaaa gcgcgacagc aattcgtcgc gcgtgatggg attattgctg cgaaacggca
     5641 cctttacctg accagataat ttcgcccgtc tgcaccagca tcagctgcat ttgtagggtc
     5701 ggagcgttaa cgttgccaga ggcgcaggag tacagcacgt aatgagcgcc gacattgcgg
     5761 gcaatgccta tggctttact acgggtgcct aaactgtcct gcggcgacaa acctaactgt
     5821 tgcttagcca tcgacagttg ctgggcggaa accagggtaa atttcccgtt attagccagc
     5881 gcatttcgca gcgtttcggt cgcttctgcg gcattcagcg aaccgttagt acggttgtta
     5941 acgctatcaa ccagcaggac gctacccgca gtcaccccgt cagccccaag catcttactg
     6001 accatcggct gcattgcgcc attccagtca taatggcgaa tatgcggcgc aggcggtgcc
     6061 gttcgatctt cgtgctcaat tgggcctggc tgctgcggga tcgtcggcac cgagggcact
     6121 gtggggacag gctgttgtgg ctcggctggt tgttccggcg ctggtttcac ttcttctacc
     6181 ggtgcaggtt cacgttgccc cacacacccg gcgagaaaca tcgccagcgc ggtaatcaag
     6241 gcgtagcgac tcatttttgt catcaagatt caccccttta caaatataga taaagtctga
     6301 ctttgtgcgc ccccagaaaa ttggcgctgc cgtacagcgt taccgccgaa tgtgcgggaa
     6361 tggtaacgct gcgtggcctt tccagaggat gcatctccag ccctctggcg tcataccagt
     6421 aaaaacgata atgaacggta acgggttctt gcctttcgtt ataaatcgtt gaggatgctg
     6481 aaggttgaat atcagacgtc gaaaggacgg gcttttctgc actgatgccc gcagccaata
     6541 aagatgactc catcaccagc gattgctcat cattcaccgg aatttccgga tgtgaacgac
     6601 agcccaccag cagtaacaac accagagaca ccagcccaaa gcatcctttt ctcatcgtta
     6661 cagaccttta tgcgccagca ttggtcccag cggacggcca cccaacaagt gcatatggat
     6721 gtggtaaacc tcttgtccgc catggcggtt ggtattcatg atcagacgat agccatcttc
     6781 ggcaatacct tcttgctcag caatttttgc cgctacggtg atcatgcgtc ccagcgcctg
     6841 ctcatgctca gctgacacgt cgttcacagt cggaatgaga atattcggaa tgatcagaat
     6901 atgcgttggc gcttgcggcg aaatatcgcg aaacgccgtt accagatcat cctggtagac
     6961 gatatcggag gggatctcac gacgaataat tttgctgaat atagtttctt ctgccacgac
     7021 attttccttt ttcaaatagc catgcgttgc gccatgctac gccgcgccac actgcgagta
     7081 tagagtatgg tcgagttaaa cctgctcttt caatcttaac tcacgatttc ttaagcaaaa
     7141 aatcgttttg ctgccgtata tatcgccatt attcccattt ctgcctgcga atgcgctgaa
     7201 aagacattct ctgtttggtt tacacgacaa atatgaatgc gtatatttct catttgcatt
     7261 tgcatttgca gcaaaattat tcgcacacta aatataaccg ctcatcaccc tcctccggca
     7321 tgagcctgac gacaacataa accaagagat ttcaaatgct ttcaacacaa tttaacaggg
     7381 ataatcaata tcaagtcatc accaaaccgt cactacttgc cggttgcata gcactggcac
     7441 tattaccttc tgccgctttt gctgcaccag ccactgaaga aacggtgatt gttgagggtt
     7501 cagccacagc tccagatgat ggcgaaaatg attacagcgt aacgtctacc tctgcgggta
     7561 ccaaaatgca gatgactcaa catgatattc ctcagtcggt cactattgtt agccagcagc
     7621 ggatggaaga tcagcagtta caaacgctgg gcgaagtgat ggaaaacacg ctggggatca
     7681 gcaaaagtca ggcggattcc gatcgtgctc tttattattc ccgcggattc cagatcgata
     7741 actatatggt tgatggtatc cccacctatt ttgaatcgcg ctggaatctg ggcgacgcac
     7801 tttctgatat ggcactgttt gaacgcgtag aagtagtgcg tggcgcgaca ggactcatga
     7861 ccgggacggg taatccatct gcggcaatta atatggttcg aaaacacgcg acctgtcgtg
     7921 aatttaaagg cgatgtctcg gcggaatacg gtagctggaa caaagaacgg tatgtggcgg
     7981 atttacaaag cccactcacc gaagacggta aaatccgcgc gcgaattgtc ggcggctacc
     8041 agaataacga ctcatggctg gaccgctaca atagtgaaaa gaccttcttc tcgggcattg
     8101 tcgatgctga tttaggcgat cttacgacgc tttcagccgg ttacgaatat cagcgcattg
     8161 atgttaatag cccaacctgg ggcggtttac cgcgctggaa tactgatggc agcagcaaca
     8221 gttacgatcg cgcacgcagt accgcacctg actgggcgta caacgataaa gagatcaaca
     8281 aggtctttat gaccctgaag cagcggtttg ctgatacctg gcaagcgaca ctgaatgcca
     8341 cccactctga agtcgaattt gacagcaaaa tgatgtatgt cgatgcctat gtaaacaaag
     8401 cggatggtat gctggttggg ccatacagta attatggacc tggctttgat catgtcggcg
     8461 gcaccggttg gaacagtggc aaacgtaaag ttgatgcgct ggatttgttc gctgacggta
     8521 gttatgaatt gtttggtcgt cagcacaatc taatgtttgg tggcagttac agcaaacaaa
     8581 acaatcgttc agttcatggg ccaacatctt cccggatgaa attggcagtt tctacaactt
     8641 taatggcaat ttcccacaag ccgactggtc atcacagagc ctggcgcagg acgataccac
     8701 acatatgaaa tcgttatatg ctgccactcg tgtcaccctt gccgatccgc tgcatctgat
     8761 cctcggcgca cgttatacca actggcgggt tgatacgctg acttacagca tggagaaaaa
     8821 ccacaccacg ccttacgctg gtctggtgtt tgacatcaat gacaactggt cgacctacgc
     8881 cagctatacc tctattttcc agccgcaaaa tgatcgtgac agttcaggca aatatctggc
     8941 tccaatcacc ggtaacaact acgagctggg tctgaaatcg gactggatga atagccgtct
     9001 gaccaccacg ttagccatct tccgtattga gcaggataat gtcgctcagt ccaccggtac
     9061 acctatcccc ggcagcaacg gcgaaaccgc ctataaagcg gtggatggga cagtcagtaa
     9121 aggggtggaa tttgaactca acggcgcaat taccgacaac tggcagctga catttggcgc
     9181 aacgcgctat cttgcagagg ataacgaagg aaacgccgtt aatcctaatc tgccacgcac
     9241 cacggttaaa atgttcacca gctatcggtt gcctgtcatg ccagagttga cagtcggcgg
     9301 tggtgttaac tggcaaaatc gcgtgtatac cgacaccgtg acaccgtatg gcaccttccg
     9361 cgccgagcaa ggtagctacg cgctggtgga tctcttcacc cgctaccagg tgacgaaaaa
     9421 cttctcgtta caggggaacg tcaataacct gttcgacaaa acctacgata ccaacatgga
     9481 aggttctatc gtctacggcg caccgcgtaa tttcagcatt accggcacgt atcaattctg
     9541 aataacacct gtaaaaaagg cagccatctg gctgccttag tctccccaac gtcttacgga
     9601 ttagtggtta cggatgtact catccatctc ggttttcagg ttatcggatt tagtaccgaa
     9661 aatagcctga acaccagaac cagcaaccac tacgcccgct gcgcccagtt tcttcaggcc
     9721 agcctgatcc actttagaca catcagcaac gctgacgcgc agacgggtaa tacatgcgtc
     9781 gaggttagta atgttttctt taccaccaaa tgcagcaacc agagccggtg ccatttcgct
     9841 ggtacctgtt gcttttgcat cttcagtcgc gtcttcacga cccggcgttt tcagatccag
     9901 tgctttaatc agcacgcgga agatggtgta gtaaacaatc gcataaccga taccgacgat
     9961 cggtaacagc cacagtttgc tgctgttacc agacagaacg atgaagtcga tcagaccgtg
    10021 cgagaacgac gtaccgtcac gcatccccag aagaatacag attgggaatg ccaggcctgc
    10081 cagaatcgcg tggatgatgt acaggatcgg cgcaacgaac atgaagaaga actcgatcgg
    10141 ctcggtgata ccggtcagga acgaggtcag cgccgcggag atcataatac cgcccacttt
    10201 cgcgcggttt tctggtttag cagagtgcca gatagcaatt gcggcagctg gcagaccgta
    10261 cattttgaac aggaagccac cagacagttt acccgcagtc gggtcacccg ccatataacg
    10321 cggaatgtcg ccgtggaaaa cctgacctgc tgcgttggtg tattcaccaa tctgcatctg
    10381 gaaaggtacg ttccagatgt ggtgcagacc aaacggtacc aggcaacgtt cgatgaaacc
    10441 gtaaatgcca aacgcaacta ccgggttctg gtaagcagcc cactgagaga aggtctggat
    10501 tgcagaacca atcggcggcc aaatgaagga cagcacaacg ccagtaaaga tggcagccag
    10561 gccagaaatg atcggcacaa agcgtttacc ggcaaagaag ccaagatact caggcagctt
    10621 aatacggtag aaacggttaa acatgtacgc tgcaatcgca ccggagataa tccctccgag
    10681 tacgccagta tccgccaagt gtttagaggc gatttcttca gcaggtaaat gcagtaccag
    10741 tggcgcaacc acggccatgg ttttaaccat gatgccatag gcaacaactg cggccagcgc
    10801 ggatacgcca tcgttattgg taaagccgag ggcgacaccg atcgcaaaaa tcagtggcat
    10861 gtttgcaaag acggaaccgc ctgcttctgc cataacatgc gatacaacgg cgggcagcca
    10921 gctgaaattc gcggaaccga cgcccagcag aatacctgcg ataggtagta cggataccgg
    10981 cagcatcagc gatttaccga ccttttgcag gttagcaaat gcattcttaa acataattga
    11041 gagtgctcct gagtatgggt gcttttttta cgttctcacg cgtggcaagg ggggagagcc
    11101 tcgccgtgta cagggcatct aagcgccctt tatttattac acagagtaaa ataattcagt
    11161 gccaatttgt ttgacggcta tcacgtttca acttcagatc attggagaag ttgcacattt
    11221 tgacaaaaca tatgtcacaa tatttcaaac gcggttattc accgcccaat tttcggcggt
    11281 gaactttact cgttttagcc attaattacg agctttaaaa aaactcattc aacggatgga
    11341 ttgaaggcgg gaagcgtcga tgtgaaacag acgggcgaag ttatcggtgg ttacctgcgc
    11401 cagttcttca acggcaacac ctttcaacac agccatgtat tctgcaacgt cacgaaccat
    11461 cgcaggttga ttctcttttc ctcgatgcgg taccggcgca aggtaaggtg agtcagtttc
    11521 caccagtaat cgatccaggg ggacataacg cgcagcatcg cgcagttgct ccgcattacg
    11581 gaaggtcaca atgccggaaa aggagatgta aaatccgaga tccagtaatt tacccgccgt
    11641 ttctctgtcc tctgtaaaac agtgtagtac gccaccgcaa tccgtcactt tttcttcgcg
    11701 cagaattgcc agcgtatcgg cacgggcgtc acgggtatgg acgataaccg gcttgttcag
    11761 ttcacgacca atctggatat gatggatgaa ggactcttgc tgacgtactt tagtttccgg
    11821 tgtgtaataa taatccagcc cggtttcacc cagcgctaca acaccctctt ctgccgccag
    11881 acggcgtaaa tcttctacat cgtagggatc attctggttg agtggatgca cgccacaaga
    11941 aaatacgacg ttgtcacgtt cgcctaccag atcccgcata tgtaaataac ccggtaatgt
    12001 tgtggcgact gccagacaaa atttcacatc gcgtgcggcg gctttcgcca gaacgtcatc
    12061 cacgtcctta tgcaaagatt cataatccag accatcgaga tggcagtgtg agtcgactaa
    12121 aaacatgatg tctctcttaa agatgaggaa ccggtaacac aacgcccggt tgcaggtaat
    12181 gctcaatacg cagtaaaaga tcggtgatga gaagctcgcg gttgatgcct gtgacagaca
    12241 ttaactgttc acgaatgtgg caaacatccc ccagtatagc ctgcaggcac gagggagaaa
    12301 gatggtttgc cagttcggcg accaggcccg gcacatcaac attggtcacc tgcgcagcgc
    12361 catgatggcg ttttagcgca tccatcagca acgttgccag ccagtgtaaa cgcgccggag
    12421 cttgttcatg attaagggcc gccagcagcg aataccagtc gcccgatggc acgctatatg
    12481 ccaacgcctg acacaatgtt tcacgagcct gccagttatc tccctgaaac aacgccagtg
    12541 ccgcgccagg cgaaccggcg cttaagcgca atgcggcaag taatgcatcc tgtgacattg
    12601 tcacttcgcg tgaaagccaa gtcacggcgt actgttctgg aggcggcgca aggtaatgta
    12661 accgacaacg actacgtaat gttgccagta aacgttcagg ctcgcgggta gccaggaaaa
    12721 accaggtttc tgctggtggc tcttcaagcg ttttcagcaa tgcgttagcc gcggcgtcgg
    12781 tcagtaaggc agcatcggtt acccagacaa ctttcgcacc acctaggcgt gcgtgctcat
    12841 tcagcttttc ggtgacctca cgtaccgcat caatgcccag cgtatttttc cctttttcgg
    12901 gagccagggt gtagtaatcg ggatgcgtgc cagcctgcat caactgacat ccacgacagt
    12961 gaccgcaact tttgtggccc tgcggttgtt ggcagagtaa ataacggctc agggcgtaga
    13021 ttaaagcatc atcgcccatg cccggtaacg cctgaatgag tagcgcatgg tgacctcttc
    13081 cggcctgata gctggctacc agtttttcga aatcaggtcg taaccatgga taccatctca
    13141 tgcgtccaac tccttcaccc agtgggtcac ggtagtgcgg attgcatcca tcacggcctc
    13201 cagcggctgg gtggcatcaa tggtatgaat acttttatct tgtgcagcca gttccagata
    13261 gcgggcgcgg gtgcgattaa agaaatcgaa agactcttgc tcaatacgat ccagctcgcc
    13321 gcgcgcacgc gcgcgtttta agccaacttc cggggtaaca tcgagataga gcgttaagtc
    13381 tgggcgaaaa tccccgagaa cagcatcacg cagtgttgcc agcatatgtt ggtcaatacc
    13441 acgtcctccg ccctgatacg cctgagtgga aagatcgtgg cgatcgccaa tcacccaggt
    13501 gccgttagcc agcgcaggtt tgatgaccgt ttctaccagt tgaacgcgcg cggcataaaa
    13561 catcagaact tcggctttat cggtaatgac ttcatcgcct accgatttga tatccagcac
    13621 caggcttctt aacttttcgg caagttgcgt accgccaggt tcccgagtga aaaccatgtc
    13681 gcggataccc agttgctcga gcgtctcaac caccacatta cgcgcggtag ttttgcctgc
    13741 gccttccagc ccctcaatga cgatatactt actgcgcatt tttttcctta agcactttca
    13801 gataatcctg cacagacttg ttatgactgg caagattggt attaaacgtg tgaccacctt
    13861 taccatcggc cacaaaatag agatacggcg tttttgccgg atgcgcagca gccttcagcg
    13921 aatccgcccc cggcgtagct atcgcacccg gcggcaaacc ggtaatggta taggtgttat
    13981 acgctgtcgg cgtttccagg tctgcacgag aaagtttgcc attataacgc tctcccatcc
    14041 cgtaaatcac ggttgggtcg gtctgcaggc gcataccaat gcgtaaacgg ttgataaata
    14101 ccgaggcaac ctgatcgcgt tcactggcaa cggcggtttc tttttcgata attgatgcca
    14161 tcgtcaccag ctgattttta tctttataag gcagaccgtc cgcacgccct tcccaggcgc
    14221 tatcgaccgc tttcaccatt ttcttgtgaa ctcgcttgag taacgcgaca tcggtggtat
    14281 tggcggtata catcaaggtg tctggccaga accaaccttc aatccactcc gggttttcca
    14341 gttcaagtgc ctgcgctacg gtggcgtact tatcgtcact cagcgtgtgc ttgatatacg
    14401 gagcctcacg caattgcttg aggtagtcgc tcagacgcat cccttctacc agtcgcagcg
    14461 ggaactgtgc ttctttaccg ctttccaaca atttcagcat ctcgcgcacg gtcatctgcg
    14521 gtgtaaagcg gtaagtcccg gctttaaagt gagaaagatc cggttcgata cgcagcagcc
    14581 attgaaaaac ccgtgggcga ttgatgatct tatcagcata aagctgttca ccgagcgcca
    14641 gacgtccggt ccctggcttc agggtaaata tcgtctcttc tttgataagc aatttgctgt
    14701 cggcaagatg gcgaaccttc cagacgccca caccagcggc gatacccagt accaccagca
    14761 ataacaagat aatcaataac acttttttca tgactaattc gggcgctcac aaagtggggc
    14821 taaatattca tataacgttg ctgacgaaaa ggtgacatcg ccacaggcac gtacgggcat
    14881 cactggcatt aacgcattac aaataaccat ctcatctgcc tgcaacgcct cctccagcga
    14941 ggcttgcact tcgacaagct gataagagga ttgtgccagt aaacggatac agaattgtcg
    15001 cataatgccg ttaacacctg cctgatccag tcgcggcgta taaactacat tgcccttccg
    15061 ccagaacaaa ttagccgcac agcattccgt aacccaccct tcgctgtcaa ggaccagcgc
    15121 ctcatcagcg tttgtctgct caagatgaga gcgaatcaat acttgctcaa gccgattaag
    15181 atgtttaata cctgcaagat gaggattgcg ccccagccgc accgggctta gcgccaacgt
    15241 catcccttcg ttacgcaaac ggtcgtaatg tgcaggataa gccgtaacgg agagaatccg
    15301 cgttgctggt ccgctgttca atgtgctgta ccctcgcccg ccactaccgc gactgatcac
    15361 gactttcagc acaccatttt gctgttctgc tgccagcgtt ttcatctctt gttcaagctg
    15421 aggccagaag tcacaggaaa tcatcaaccg ctgacaagca tcctgtagtc gcaggatatg
    15481 cgccgataac aaactgactt taccgtcgat aactctggcg gtggtaaaac aaccatcacc
    15541 aaactgcgtt gcccgatcgc ttactgccag cgattcctgc ttataaccgt taattaagaa
    15601 cataccggct ccttatcaga gaattgttag tgtggcagca tgttcactac ggaacaagtc
    15661 ggaataaaag ctaagaaaaa aggcccgcaa gcggaccttt tatatgggtg gaaaatgaca
    15721 acttagatct ttttaaagat caaagaacca ttagtgccac cgaagccgaa ggagttacac
    15781 agcgtgtatt ccattccgct gacctgacgt gcttcatgcg gtacgaaatc cagatcgcaa
    15841 ccttcatccg ggttatccag gttgatggtt ggcggaacag cctgatcgcg cagcgccagg
    15901 atggagtaga tagattctac tgcacccgcc gcacctaaca ggtgaccggt catagatttc
    15961 gtggagctta ccaacacacg gcttgcagct tcaccgaaga tggttttcac cgcctgcgct
    16021 tcagctttat cgccagccgg cgtagaagta ccgtgcgcgt taacgtagcc aatctgactc
    16081 gcttcaatgc ctgcatcacg cagagcattt gccattgcca gagctgcgcc tgcgccattt
    16141 tctggcggtg acgtcatatg ataagcatcg ctgctcatac caaagccgac gagttcagcg
    16201 taaattttcg caccgcgttt tttcgcgtgt tcgtactctt caagtaccag cataccggca
    16261 ccatcgccca gtacgaagcc atcacgctct ttatcccacg ggcggctcgc cgcttgcggg
    16321 ttatcattgc gggtagataa tgcacgtgcc gcgccaaaac caccaacgcc cagcggcgta
    16381 ctggctttct ctgcgccacc tgcaaccatc acgtcagcat cgccatacgc gataatacgc
    16441 gcagcatggc caatgttgtg cacgccggaa gtacaggcgg tcgcgataga gatgcttggg
    16501 ccacgcaggc catacatgat agtcagatga cctgccacca tgttcacaat cgttgacgga
    16561 acgaagaatg ggctgatctt acgtggacca ccgttcatca gagatgtgtg gttttcttcg
    16621 atcagtccga ggccgccaat cccggagcca attgcggcac caatgcgggt tgcgttctct
    16681 tccgttattt caaggccaga atcctgcatg gcctgaacgc cagcgacaat tccatattga
    16741 atgaaggcat ccatcttgcg ctgttctttg cgcgagataa tgtcctcaca gttaaaatcc
    16801 tttactaagc cagcaaattt cgttgcatag gcgctagtat cgaaatggtc gattaggctg
    16861 atgccactct gaccggcaag cagagctttc caggtagact ctacggtatt gccgacagga
    16921 gacaacatgc ccagtccggt cacaactaca cgacgcttag acacgtttgt cctccaagga
    16981 gggaaaaatg attctagtgg gacaaaaaga taaaactcag gcggtcgaac gaccgcctgg
    17041 agatgttcac ttacgcctgg tggccgttga tgtaatcaat ggcagcctga acggtggtga
    17101 ttttctcagc ttcttcgtcc ggaatctcag tatcaaactc ttcttccaga gccattacca
    17161 gctcaacggt gtcaagagaa tccgcgccca ggtcttcaac gaaagaagca ttgttggtaa
    17221 cttcttcctg cttaacgccc agctgttcgc cgataatttt cttaacgcgt tcttcgatag
    17281 tgctcatact cttaaatttc ctatcaaaac tcgctttcgc gatggttttc gtagtgtata
    17341 aaatgttgaa aaatttgcaa ctaaatcccg gcaggtctta ccacgatttt acgttatttt
    17401 gaggccttac cccccaataa cgcaaatcat tttgcactaa ttattactca taaccacgca
    17461 gactgcgcgc aacatgagca ttttgtgcaa atcgcggtca gaccatgtac atcccgccgt
    17521 tcacatgcaa agtttcaccc gtgatgtaag ctgcttcgtc ggatgccagg aatgcaaccg
    17581 cgttggcgat ttcctgtgcg ccgccgaggc gacccgcagg aacctgcgcc aggatacccg
    17641 cacgctggtc atcgctcagc gcacgtgtca tgtccgtttc aataaagccc ggagcaacaa
    17701 cgtttacagt aataccgcgt gacgcaactt cgcgcgccag tgatttactg aagccgatca
    17761 agcccgcttt cgccgcagcg tagttggcct gaccgccatt tcccatggta ccaaccacag
    17821 aaccgatagt gataatacga ccatgacgct ttttcatcat agcgcgcatt accgcttttg
    17881 acagacggaa aacagatgaa aggttagttt cgataatatc gttccactct tcatctttca
    17941 ttcgcattaa caggttatca cgagtgatac cggcattatt gaccaggata tccacttcac
    18001 caaattctgc gcgaattttt tccagaacag attcgataga tgccgggtcg gtcacattca
    18061 acatcagacc tttgccgttg gcacctaaat aatcactgat cgcctgagcg ccattttcac
    18121 tggtcgcagt gccaataact ttcgcgccac gggctgcgag cgtttcagca attgcgcggc
    18181 caattccgcg gcttgcaccg gttaccagtg cgatttttcc ttcaaaattc atgattttcc
    18241 tcttttaaag ctcgagcgcc gctgccatcg ctgaaggttc gttcagcgcc gaggcggtca
    18301 gggtgtcgac aatgcgtttc gtcaggccag taagcacctt gcccgggccg acttcataga
    18361 gatgttctac gccttgcgct gccatgtact caacagactt cgtccactga accgggttat
    18421 acaactgacg taccagtgcg tcacggatgg catcaccatt ggtttcgcat ttcacatcaa
    18481 cgttattcac aacagaaact gttggtgcgt taaaggtgat tttcgctaat tctactgcca
    18541 gtttgtcggc tgctggtttc atcagcgcac agtgagacgg tacgctcact ggtaacggca
    18601 gcgcgcgttt tgcgccagca gctttacagg cagcgccagc acgctcaacc gcttctttat
    18661 gaccggcaat aaccacctgt cccggagagt taaagtttac cggagaaacg acctgacctt
    18721 ctgcagcttc ttcacacgct ttcgcaatag gcgcatcatc cagaccgatg attgccgcca
    18781 tagcgcccgt gccttccggt acggcttctt gcatgaactt accgcgcatc tcaaccagac
    18841 gcaccgcatc agcgaaatca atcacgccag cgcaaaccag cgcggagtat tcccccaggc
    18901 tgtgaccggc catcattgcc ggtgctttac cgccctgctg ctgccatacg cgatacagcg
    18961 caacagatgc agtcaacagc gctggctggg tttgccaggt tttattcagt tcttcagctg
    19021 acccctgctg ggtcagcgcc cacaggtcgt agcccagcgc cgcagaagct tcagcaaacg
    19081 tttcttcgac aattggatag ctcgccgcca tatcagccag cattccaacg gtttgagaac
    19141 cctgtccagg gaacacaaat gcaaattgcg tcatgtttta atccttatcc tagaaacgaa
    19201 ccagcgcaga gccccaagtg aatccaccgc caaaggcttc aagcagaacc aactgccccg
    19261 gcttaatgcg cccgtcgcgt acagcttcat ccagcgcgca cgggacagag gccgcagagg
    19321 tattaccgtg gcgatccagc gtcaccacga cattgtccat cgacataccg agttttttcg
    19381 ccgttgcact gataatacgc aggttagcct gatgcggaac cagccagtcc agttgagaac
    19441 ggtcaagatt attcgccgcc agcgtctcat caacgatgtg cgccagttcc gttaccgcaa
    19501 ccttgaagac ttcgttgccc gccatcgtca gatgaattga attctctgga ttcacgcggt
    19561 cggcgtttgg cagcgtcagc aattcaccat aactaccgtc ggcatgcaga tgggtggaaa
    19621 tgattcccgg ctcttcagag gcagccagca ccgcagcgcc cgcgccatcg ccaaaaataa
    19681 taatagtccc acgatctgtt ggatcgcagg tgcgcgccag tacatcggaa ccgacgacca
    19741 gagcatactt caccgcccca gatttcacgt attgatcggc tacgcttaat gcataggtga
    19801 aacctgcaca ggctgctgca acgtcaaatg ccgggcaacc tttaatgtcc aacatgcttt
    19861 gaatctgaca agctgcgctc gggaaagcgt gcgtagcaga agtcgttgcc acaacgatca
    19921 ggccaatctg gtctttctca atgcccgcca tctcaattgc gcgtgtcgcc gcttcaaagc
    19981 ccatggttga aacggtttcg tttggcgcgg caatgtggcg ttcgcggata ccggtacgag
    20041 tgacaatcca ctcgtcagag gtgtccacca ttttttccaa atcggcgttt gtccgcactt
    20101 gttcgggcag atagctgcca gtaccaataa tcttcgtata catgtacgct cagtcacttt
    20161 tcggttatat accgtcactt gcaaactgcg agttcgctgg cagcgtcctg ctaccgcaga
    20221 gttccgcttt tgccaccgtc cagcagctca aaaccagctg ggtatacaga ttccaggcga
    20281 gcggcaattc gctgaggaac ttgtcgctgc accgcctgca ctgcctgttc aatcgcgacc
    20341 gcaaaagctc gctgattggc tgcaccatga ctttttatca ccgtgccgcg caatcctaac
    20401 agacaggcgc cgttatactg gtcggggttg aggtgactga atcgcctcgt caggctcttt
    20461 tgtagccaac gctttaataa cagtagccac cacgaccgtt ttttcccttc accctgagat
    20521 ttcagcagag aaaggaacat cctgacaaca ccttccatcg tctttaacgt gacatttcct
    20581 gtaaagccgt cacaaaccag cacatctgtc ttgccagtta acaactcatt ggcttcaaga
    20641 tagccgatat aattgataga agggattgtt ttaagcaccg ctgaggcatc ccgaatactg
    20701 tcaagaccct ttacttcttc ttcaccaata ttgagcaacg ccacgcgagg attgggaatt
    20761 tccaccacct cttcagccag aactgagccc ataatggcaa attgcaccaa cattgtgctg
    20821 tcacaatcga cgttggcccc taaatcgagg accaccgttt tgcccttttg ctgatgtggt
    20881 aataccgtca ccagcgccgg acgctcaatc ccctccaggg gcttgagtaa taattttgcc
    20941 agccccatca gtgccccggt attaccggca ctgacacagg cttgcgctcg accttctttc
    21001 accagctcca gggccatacg cattgaactc ccacggctgg cgcggatagc ttgcgaaggc
    21061 cgggcatcac tggcgataac tgactgcgca ggaataatct gcagacgcga acgttgttca
    21121 aagtcagctt tagcaagtaa tggcgtgatg gcgtcgggat tgccgactaa aagaagagtg
    21181 agttgcgaat tagaattcag tgcctgcaat gctgcaggca ctgtcacgga agggccaaaa
    21241 tcccctccca tgacatctaa cgccagggtt agacgtgtca aggtatcgtc gccgcccggt
    21301 ttggtctttc cccagttgcc tggggaaaat cctcactaag cttcatcacg cagacgcgtg
    21361 attacttagc gatgaccttg cggccgcggt agtaaccgtc ggcagtgatg tggtgacgca
    21421 ggtgtttttc accagaagtt ttgtctacag acaggctggt gactgcggtc agcgcgtcat
    21481 gggaacgacg catgccacgt ttggaacggg ttggtttatt ctgttgtacg gccatggacc
    21541 ttactcctca attacggttt atccccgatc caacggggag caccaattac ttacgcttta
    21601 agctggctaa tacggcaaat gggtttggct tttgcacttc ttcaggcagt tcaccaaaga
    21661 ccatgtccgc ttcggacact tcacagtgtt cagaatcatg caccggaact accggcaagg
    21721 cgaggatgat ttcatcttca accattgcaa gcagatcgat ttcaccgaat tcgttaacct
    21781 caatcggttc atacgcttcc ggcagtgctt cagcctgttc gtctgaacgc acaggactaa
    21841 aacaatacgt tgtgtagacc tgatgagtaa acggcttccc gcaacgctga cactcgagcg
    21901 ttaccgtcac cttcgcatcg ccgtttaaca ctgcgagacg ttggttatcg atagcgaacg
    21961 acatggagca ttccacatca ctgtccacac tgactacgga ttcggcgacg cgctcaacct
    22021 gatcaggggt atagataccc tggtaatcaa ggcgtttttg agccgtacga accggatcga
    22081 gagtcagggg taattttacc ttttgcatag ggcgcgcata ttaactttgt aacgtcatag
    22141 agtcaaagaa aaaggcagcc tgtggttgcc ttttgccaat aattcgcaca cattgcgggt
    22201 tactgcttta ttttcgttca gtgctgataa cgaacatcaa cctgcaaaac gaagcgcata
    22261 gtttaaaatg gctatcatca atacgctata tctgatggaa aaaaatatgc ctaaacttat
    22321 tttagcctcc acctcgccat ggcgtcgcgc tcttctggaa aaactgcaaa tctcttttga
    22381 atgtgcagca cctgaggtcg acgaaacccc acgcagcgac gaatcaccgc gacagttggt
    22441 gcttcgactg gcacaagaaa aatcgcaatc tctggcgtca cgttatccgg atcatttaat
    22501 tattggttca gaccaggtat gtgttcttga tggcgaaatc accggtaaac cgttaacgga
    22561 agaaaatgcc cgtctgcaat tacgcaaagc cagcggcaat atcgtcacct tctatactgg
    22621 actggcgttg tttaattcgg cgaatgggca tctacaaaca gaagtggagc cttttgacgt
    22681 ccatttccgt cacctaagcg aggcggagat tgataattac gtgcgtaagg agcatcccct
    22741 gcactgcgcg ggtagcttta agagtgaagg atttggcatt acgctgtttg agcgtttaga
    22801 gggacgtgac cctaacacgc tggttggttt accgctaatc gcgctgtgtc agatgttacg
    22861 tcgggaaggg aaaaacccgc tgatgggata gttgtttgcg ccggatgcgg ttacgtcgca
    22921 tccggcaatc ggtgcaggcg gcctgatgcg gcgtaaacgc cttatcaggc caaccgccca
    22981 ttatcaggct tatatgcttg tattagcgcg cgttacgcag cttttgcaaa caacgcttca
    23041 aaccttcatc catcggcgct tcgatacgca tcacctcacc ggtccccgga tgggtaaact
    23101 tcaacgctgc ggcgtgcagg aacagacgat ttaatcccgt gcctgcttca gtgagctgtc
    23161 tgtcaaattc acggtcaccg tagcgatcgt caaaggcaat cggatgaccc gcatactgtg
    23221 tatgcacacg gatctgatga gtacgtccgg ttaccgggct acaacgcacc agggtggcaa
    23281 atgcatagcg ttcttccact ttaaagcgtg tttccgacgg tttgccttcc tgactgacac
    23341 gcacgatacg ttcgccgctt tgcagaatat ttttcaataa cggcgcttga acgctcttca
    23401 catgcgactg ccactgaccg cgcaccagcg ccaggtaatc tttttgcatt cctttttcac
    23461 gtaattgctc atgcagagaa cgcaacgccg agcgtttttt cgctaccagc aacacacctg
    23521 aagtgtcccg gtcgagacga tgaaccagtt caaggaaccg cgcttccggg cgcaacgccc
    23581 gcaaaccttc gataacgccg aagcttaaac cactgccgcc atgtaccgcc gtaccggaag
    23641 gtttattcag caccaggata tgatcatctt catataagat gacgtccgcc agcgccgcca
    23701 ccttttgcag atgtggcgaa accgcctctt cttcccgctc agcaacgcga accggtggga
    23761 tacgcacctc atcacccgct tcgagtttat attcaggctt aatacgtttt ttgttcaccc
    23821 gcacttcacc tttacgcaaa atacggtaaa tcatactttt tggtacgcct ttcaattggg
    23881 tacgcaaaaa gttatcgata cgttgccccg cttcgtcagc ggtgatagca acaattttta
    23941 cggatggagt ctctgttttc atggtgggcg attctaatta gccaacagga ttcgcgccac
    24001 tcatttttct atgcttatat ttagttttgc accttattac ttcactacgt gatcacttta
    24061 ttgatggtta ttaaaccaat caccagcaag aagtgaaaaa actgtgagta agcgggtgat
    24121 aaatggtaaa agtcatcttg ctataacaag gcttgcagtg gaataatgag gccgtttccg
    24181 tgtccatcct tgttaaaaca agaaatttta cggaataacc cattttgccc gaccgatcat
    24241 ccacgcagca atggcgtaag acgtattgat ctttcaggca gttagcgggc tgcgggttgc
    24301 agtccttacc ggtagatgga aatatttctg gagagtaata cccagtctgt ttctttgata
    24361 attgcgctgt ttttccgcat gaaaaacggg caaccgacac tctgcgcctc tttgagctga
    24421 cgataaccgt gaggttggcg acgcgactag acacgaggcc atcggttcac acccggaaag
    24481 gcgttacttt gcccgcagct tagtcgtcaa tgtaagaata atgagtaagt tacgatgaaa
    24541 agaatgttaa tcaacgcaac tcagcaggaa gagttgcgcg ttgcccttgt agatgggcag
    24601 cgtctgtatg acctggatat cgaaagtcca gggcacgagc agaaaaaggc aaacatctac
    24661 aaaggtaaaa tcacccgcat tgaaccgagt ctggaagctg cttttgttga ttacggcgct
    24721 gaacgtcacg gtttcctccc actaaaagaa attgcccgcg aatatttccc tgctaactac
    24781 agtgctcatg gtcgtcccaa cattaaagat gtgttgcgtg aaggtcagga agtcattgtt
    24841 cagatcgata aagaagagcg cggcaacaaa ggcgcggcat taaccacctt tatcagtctg
    24901 gcgggtagct atctggttct gatgccaaat aacccgcgcg caggtggcat ttctcgccgt
    24961 atcgaaggcg atgaccgtac cgaattaaaa gaagcactgg caagccttga actgccggaa
    25021 ggcatggggc ttatcgtgcg caccgctggc gtaggcaaat ctgctgaggc gctgcaatgg
    25081 gatttaagct tccgcctgaa acactgggaa gccatcaaaa aagccgctga aagccgcccg
    25141 gctccgttcc tgattcatca ggagagcaac gtaatcgttc gcgccttccg cgattattta
    25201 cgtcaggata tcggcgaaat ccttatcgat aacccgaaag tcctcgaact ggcacgtcag
    25261 catatcgctg cattaggtcg cccggatttc agcagcaaaa tcaaactgta caccggcgag
    25321 atcccgctgt tcagccacta ccagatcgag tcgcagatcg agtccgcctt ccagcgtgaa
    25381 gtccgtctgc catccggtgg ttccattgtt atcgacagca ccgaagcgtt aacagccatc
    25441 gacatcaact ccgcacgcgc gacccgcggc ggcgatatcg aagaaactgc gtttaacacc
    25501 aacctcgaag ccgccgatga gattgctcgt cagctgcgcc tgcgtgacct cggcggcctg
    25561 attgttatcg acttcatcga catgacgcca gtacgccacc agcgtgcggt agaaaaccgt
    25621 ctgcgtgaag cggtgcgtca ggaccgtgcg cgtattcaaa tcagccatat ttctcgcttt
    25681 ggcctgctgg aaatgtcccg ccagcgcctg agcccatcac tgggtgaatc cagccatcac
    25741 gtctgcccgc gctgctccgg taccggtacc gtgcgtgaca acgaatcgct gtcgctctct
    25801 attctgcgtt tgatcgaaga agaagcgctg aaagagaaca cccaggaagt tcacgccatt
    25861 gttcctgtgc caatcgcttc ttatctgctg aatgaaaaac gttctgcggt gaatgcgatt
    25921 gaaacgcgtc aggacggtgt tcgctgcgtg attgtgccaa acgatcagat ggaaaccccg
    25981 cactaccacg tgttgcgcgt gcgcaaaggg gaagaaacgc cgaccttaag ctacatgctg
    26041 ccgaagctgc atgaagaagc gatggcgctg ccgtctgaag aagagttcgc tgaacgtaag
    26101 cgtcctgaac aacctgcact ggcaaccttt gccatgccgg atgtaccgcc agcgccaacc
    26161 ccagctgaac ctgccgcgac tgtcgtagcc ccagcaccga aagctgcaac ggcaacacca
    26221 gcagctcctg cacaacctgg gctgttaagc cgcttcttcg gcgcactgaa agcgctgttc
    26281 agcggtggtg aagaagccaa accgaccgag caaccaacac cgaaagcaga agcgaaaccg
    26341 gaacgtcaac aggatcgtcg caaacctcgt cagagcaacc gccgtgaccg taatgagcgc
    26401 cgcgacaccc gtagtgaacg tactgaaggc agcgataatc gcgaagaaaa ccgtcgtaat
    26461 cgtcgccagg cacagcagca aactgccgag acgcgtgaga gccgtcagca ggctgaggta
    26521 acggaaaaag cacgtaccac cgacgagcag caagcgccgc gtcgtgaacg tagccgccgc
    26581 cgtaacgatg ataaacgtca ggcgcaacaa gaagcgaagg cgctgaatgt tgaagagcaa
    26641 tctgttcagg aaaccgaaca ggaagaacgt gtacgtccgg ttcagccgcg tcgtaaacag
    26701 cgtcagctca atcagaaagt gcgttacgag caaagcgtag ccgaagaagc ggtagtcgca
    26761 ccggtggttg aagaaactgc cgctgccgaa ccaattgttc aggaagctcc agctccacgc
    26821 acagaactgg tgaaagtccc gctgccagtc gtagcgcaaa ctgcaccaga acagcaagaa
    26881 gagaacaatg ctgataaccg tgacaacggt ggcatgccgc gtcgttctcg ccgctcgcct
    26941 cgtcacctgc gcgtgagtgg tcagcgtcgt cgtcgctatc gtgacgagcg ttatccaacc
    27001 cagtcgccaa tgccgttgac cgtagcgtgc gcgtctccgg aactggcctc tggcaaagtc
    27061 tggatccgct atccaattgt acgtccgcaa gatgtacaag ttgaagagca gcgcgaacag
    27121 gaagaagtac aagtgcagcc gatggtgact gaggtccctg tcgccgccgc tgtcgaacct
    27181 gttgttagcg cgccagttgt tgaagaaatg gctgaagtcg tagaagcccc cgtaccggtt
    27241 gccgaaccgc aaccggaagt ggttgaaacg acgcatcctg aagtgattgc cgccgcggta
    27301 actgaacagc cgcaggtgat taccgagtct gatgttgccg tagcccagga agtcgcagaa
    27361 cacgcagaac cgatggttga accgcaggaa gagacggcag atgcagatat tgaagaagtt
    27421 gccgaaactg ctgaggttgt ggttgctgaa cctgaagttg ttgctcaacc tgccgcgcca
    27481 gtcgtcgctg aagtcgcagc agaagttgaa acggtggccg cggttgaacc agagatcacc
    27541 gttgagcata accacgctac cgcgccaatg acgcgcgctc cggcaccgga atatgttccg
    27601 gaggcaccgc gtcacagtga ctggcagcgc cctacttttg ccttcgaagg taaaggtgcc
    27661 gcaggtggtc atacggcaac gcatcatgcc tctgccgctc ctgcgcgtcc gcaacctgtt
    27721 gagtaataat tagctcaaag taatcaagcc ctggtaactg ccagggcttt tttatttcat
    27781 gtttgaatca acatattaaa attcatcaaa tggcatcctt gctaaccaac aatgcaaaat
    27841 aggcaaaaaa aatgcccgcc ccggcgatcc cagagcgggc agacaaaaca tacccagttt
    27901 catgatatgt tttaaagcga gcttatttgt tgagctggaa gagcgacaat ccctgcatat
    27961 cggtaaatgc tttatacgat gcctgcaatg ccgtttgctg catgatgtaa gatgaaatag
    28021 ttgcattcca gtccacatca accagatcgc tcatctgctg cgtttgccct aaagcgcgat
    28081 cgctacctaa ggaatccagc gactccagtt tgttcagctg cgtgcctaat tccgcgcgaa
    28141 cggtaaggac attattcagc gagtttttca gtccacggtt ggttttatcc aatgccgcag
    28201 cggcgatttc tttatccgct tcgctatccg cgaccggcgt tttcagggct gcgatggcgc
    28261 tatccagcat ggcaaaaaga ttggtttcag aagcgctacc gtctggttcc gctaccgcgt
    28321 tactggtaat actgtcgaaa attttgtcgc ccgtatgtcc tatcaccatc gaacgcgaag
    28381 catcgacctg ttgcttaata ctttctgttc cgccaacata atcaccgtta gcctcactaa
    28441 acggcgcagt ctctgtttta tatgtagcgc ccattaccgt cagtggtgtt cgccagattc
    28501 agcaactggt cacgaagccc ctgaatatcc gtagccagcg aggcccggtc atcgtcactc
    28561 aaggtgccat tgctggcgta gacaattttt tcctgagcat tctggatagc agtggtgacc
    28621 tggctaagta cactctcttc cagtgacact ttttgagtgg cgaaagtacg tgccagcgtg
    28681 tactggctgt tttgcgcctg tgcctgggag agaacaacag cttgtgatgc agcaatggga
    28741 tcgtctgaag ggttaacgac tcgcttaccc gtcgacatct gctcgccgta cttcatccat
    28801 tctgcctgag aattggtgat gccgcgcatg ttttgctggt acatcatctg ggtactgaaa
    28861 cgcattttat ctccccttag cgaatgttaa tcagcgcatc aaaaatcgcg tttgccgtct
    28921 gcagaacctg cgcattcgcc aggtaatact gctgaaaacg ttgcagattt ccgtactcct
    28981 catcgagatt gacaccggaa atcgactgct gctgattgga aagctgcgtc accacattac
    29041 cttgcgtggt gctactggtt ttcaacgtcg cggttttatt accgatatca ctcactaacg
    29101 aggcataagc gtcgttaaag gatttcgcac cgcccaccgt tttactgttg ctttgcagat
    29161 ccagcagggc ctgaccgttg cggttgtcgc tatcacccgc atcttcttcg ctcgccatcg
    29221 cgattttcgc ttcgtcggtg attaatacat ccatgttgac gatggcgtca cttactggtt
    29281 tcagcgtgaa gctgtcgtta acggcaggcg ttcctgtaaa cgtcaactcc agaccatcaa
    29341 atgccacttt accgttggca tctggtgtcg ccgtaaaagt ggtattgctg gcaaggcggg
    29401 tgacctgcca ctgattatta tcgaacgaga ttttgtaatc tgtcgccagt accacggagg
    29461 catcagttac cgtggcaccg atcgcaacgt cacctttgtt tttcgtgttt tgcagaaccg
    29521 cgggcttacc gatagtaaag aaatcttcac cagcatcgcc gttagcatca aatccggctt
    29581 tgtgttggct gttgaaagcc tcggcaaatg ccagcgccag ttgtccaagc gtattacgcg
    29641 tctggtccag atcctgagaa cggaatgtca gaatgccgcc cagcgacccg gtattcagta
    29701 atttctccgg gatctcaata ttgcctgccg tcccatcaac gtaagcgaca gtcgtacgag
    29761 aagggtcggc gctggaagga actgccgcca gttgccgcgc cgtacttccc tgaaccagtg
    29821 agtaaccatt ggcatcgtga tgttataagt accgccatcc tgaacgctga cttctacgcc
    29881 aacaatctgg tttaattcgc tcaccagttg atcgcgttga tccagcagat tgttaggtga
    29941 cgcccctgcc cccacgcctg tcaggcgcga gatttgatcg ttcaggctgg caatttgttt
    30001 agcgtaattg ttgatctgat caacgctggc acctatcgcg atattgacct gtttgtcctg
    30061 gtcgcgcaga tattgatcgg tggttttaaa ctgattcacc aacccttctg atttcccaat
    30121 cagcgcctgg cgcgccgccg ggtcttccgc gttactcacc agcgtttgca ggctggtgaa
    30181 gaaatcctgc atctgtgttg ccagcgaaga ggtactggtg gagagcatat tgtcgatttt
    30241 cgacatctgc tcatagcggg cggtcaggcc gctactttgc gtctgcgcag cacgtaactg
    30301 gttggtaata aacgtatcat actcacgctg cacaccagaa acgtagacgc cattgccaac
    30361 ccagccgcca gcgcccaacg tgctattggc ctgcgccata atagtggttt ggcgggtata
    30421 tccggcaacg ttatagctgg agatattatt acttgccgta tttaacgccg cctgggccgc
    30481 gttcagtccg ctcatggcgt tattgatcaa gctggacatg gaggttcctt ataagccttc
    30541 aattacagag tattatcggc agcgacccgc cggacttgag ttattcagaa cagattatca
    30601 atgttcatgc tgtaggtttt gctcaccttg tcgcttatcg atttcatctg ctggatcatg
    30661 ttggtgagtt tacgggcata gtgaggatcg gtagcatagc ccgcgtcctg taggacctgc
    30721 gccccctgtt ccgcactcgc ggcggtcgtc acggcggcgt agcgcgggtt acgtgttaac
    30781 agcccaacgt aatccgacaa tgcttccaga tacgagctgt agacccgaaa cttcgctttt
    30841 actttcttcg cttcgccgtt ttcatattca gtcgtggtga tttcagtgac ttgccctttc
    30901 cagttgccag aggctttgac accaaacagg ttatagcttg gctcgccgtt ttcgcggcgg
    30961 atttgccgtt gcccccaacc agattcaagc gcagcctgag cgaggatcaa atgatgtggc
    31021 acaccgcttt gctggcttgc cagttgggcg ggcagcgaga gttgcgcgag gaatgctcta
    31081 ctgtcacccg gcagcgaatc atcgtagtta cgtggtacgg ccttttgcac cagctgcgaa
    31141 agcgtctggt tttgataacg caccacggtt tcgagcggga atttcatcgg tgctgctggc
    31201 gtggactcct ctggcaatgg ttgttctggc gtcatctgtt taaccatcat ctctgcaagc
    31261 cccagacctt tgcccgccgt catctgttgg gcaatttgct ggtcatacat actggtatac
    31321 agacgagtgt gctcgctgct gaacaggcca tcttttggta aagcgtcgcg catgcttttc
    31381 aacatcatct gcacgaacat cccttccacc tgacgggcca ccggacggat atttgccgcc
    31441 ggatcttcgc tcgctttcgc ctttagttcg ttgagtgatt gcgcatccca ggccgcactt
    31501 gccagtagtt tgctgtcgct gatcatcaga tgatttccag ttttgcccgc agacatcccg
    31561 cactttgcat tgattgcaaa atagacatca gatccatcgg cgtagcgccc agcgcattga
    31621 gcgcacgcac cacgttattg aggctggcgc tggaacgtac gctttgcagc gaaccgccgc
    31681 tctggcgtaa atcgatctgc gtttgtggag taacaacagt ctgtccacca ccaaacggtg
    31741 tatctggctg gctgacattg gcctgacgat taactgttac tgagagattt ccctgcgcta
    31801 ccgcgcagct gtcgagggtc acttcgcgat tcatcaccac cgaaccggtg cgcgagttaa
    31861 tcactacttt agcgtcctgc ggggtgacat taacctgcat attctggata tcggcaagga
    31921 aacggacctg ggaactgttg ccacttggta cgcgcacctg aatagtccgc gcatctaacg
    31981 cggtggcgct gccatatcca cgcacgcggt tgatggtgtc agcgatttgc tgcgccatgc
    32041 tgaaatcttc gtcgttaagt tgcaaattaa gggtattccc gacgccaaac tggctgggca
    32101 attcacgttc aataaccgca ccattggtga tccgtccacc gttcagttgg ttaacctgaa
    32161 cactgctacc gcctgcggag gctcctgcgc cgccaaccag aatattgccc tgcgccagcg
    32221 catacacctg actgtcaacg cccttaagcg gagtcatcaa caacgtacct ccacgcaagc
    32281 ttttggcatt acccatggaa gaaaccacca catcgatggt ttgcccctga cgtccaaacg
    32341 gaggaagtga cactgtcacc attaccgcag cgacgttttt tagctgcata ttggtgcccg
    32401 tcggaacggt aattcccagc tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg
    32461 tctgggttgt ctggtcacca gtgccatcca gccccactac caagccatag ccaatcagtg
    32521 agttttgcct taccccctga acactggtga gatcgcgaat acgctcagcc tgagccgccg
    32581 tcgtaaccag tagaagaatt aatgcagaga gaaatttaat caccacagcc tcacttacat
    32641 tggcgacagg ttaaggaaga aacgctgcaa ccagcccata ttttgcgctt cgttaatgta
    32701 gccattgcct acgtattcaa tacgcgcatc cgccacctga gtagacggta cggtattgct
    32761 gccgctgata gtgcgtggat taaccacgcc cgagaagcga ataaattcgg taccctgatt
    32821 aatggcaatc tgtttttcac ccaccacatg caggttgccg ttgaccagta cctggtcaac
    32881 cgtcaccgtc aacgtgccgc taaaggtatt gctggcattg gccccgccct ttccgttgaa
    32941 cgtgttacca ccggaggctt cgacatcggc acgagcgtta ccaaacagcc cctgcaaata
    33001 gcgcggcaca gtatcaaagc caaaattagt tttaccgtca cggctggcat tcgcagagga
    33061 gcttttgctg gcgctgacgt tctcctgcaa cacgatggtc agcgtatcgc caatattgcg
    33121 tggtcgacga tcttcaaaca gcggttgata gccatagtta atcggctgag cagactggaa
    33181 aatagaaccg ttggcgacgg gcgtcggacc gggaaccggt tgtgcactgg tcgccccctg
    33241 caccagcggc gtggagggta tccaggcgca gccggttagt gaaagcacca acaagctgga
    33301 aatggcataa gtatgcgcag cgtttttttg catggctatc atcttcaaaa atcagtaaac
    33361 cggtgaacct gccaccggtt atgccttaga gttgcgtcag tttttgcagc atctgatcgg
    33421 tggtggacac cgctttactg ttgatttcgt aagcgcgttg cacctgaatc atattgacca
    33481 gttcttccgc cacgttgacg ttagacgttt caacataccc ttgatacagc agtcccgcgc
    33541 cgttcaggcc cggcgtgctt tcgttcggtg caccggagga ttgcgtttcg gtgtagaggt
    33601 tttcgccaat gctctccagt ccggtatcat tcataaaggt ggtgagattg agctgcccaa
    33661 cctgaaccgg agctgcctgg ccttgttggg ttacgctgac cacgccatca cgaccgatgg
    33721 tgatacttaa cgcattcgcc ggaatggtga tcgctggctg cacctgaaaa ccaccagccg
    33781 tcaccagctg cccgttctga tccacctgga aagagccgtc acggatatag gctgatgagc
    33841 catctggcaa cattacctgg aaaaagccct gccctttaat cgcgacatct ttgctgttat
    33901 tggtctgcga cagattgccc tggctgtgta agcgttcagt ggcgaccggg cgtacccccg
    33961 tgccgatttg taatccggag ggtaaggtgg tttgttcgga agactgtgcc cccggctggc
    34021 gaatggtttg ataaagcaga tcttcaaaca ccgcgcgctg acgcttaaaa ccgttagtac
    34081 tgacgtttgc caggttgttg gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg
    34141 ttttggcgat ccataatgaa ctgatcatat aatgtcctgt agtggattgc tgacaagcag
    34201 agcagaagca cgcgacagtg tgaatttccc ctcaccctaa ccctctcccc aaaggggcga
    34261 gggaatagtc cgcgcgcttt tattgcaatg tcatcaatcg ttcaattaac tcatcgacag
    34321 cagttggttg gcacggcctg cgttatcatc gacgctgctg atcaccttca tctgcatttc
    34381 aaaacgccgc gcgctggcaa tcatgtcgct cattgccgca acggcattga cgttactgcc
    34441 ttccagaacc cccgacatca cacgcaaggt tggatctgcc tgcagtaccg gccctcgcgt
    34501 ggcctgggtt tctgcgctta aacgaaaaat gccgtcgtca ccgcgctgca cttcgctgcc
    34561 cgtggctttc accagtttca gacgccctac tggcgcaact gtatttgccg gatcgcccgg
    34621 attgagcgcc gagattgtgc catcggcagc aatcgtgatt tccgcccctt ccggcacagc
    34681 aattggcccc gcctcgccta tcaccggatg cccctgaatt gtcagttgcc cggtgggatc
    34741 aacctgaatg ctgccattac gcgtataccc ttcgctgccg tcagcggtct gcacggccag
    34801 ccatcctgct gcaacgcaac gtccagcggg cgagaggtgt aatccatctt gccgggcgtc
    34861 atgtctgcac ccggtgttga cgccgttacc agcgtgcgcg tgggcagtga aagcccttcc
    34921 accggcactg cgcgcaaggc gtttaactgc gcgcgaaaac cgggcgtaga ggcattggcc
    34981 agattactgg cggttaccgc ctgttgattc agcgtctggc tggctgctcc catcgcagta
    35041 taaattgcgt gatccattga gctatcccgt cagcgattag cgtaagttaa cccgcgtgtt
    35101 gaggatctgg tcctgggttt tgatggtctg ggcgttagac ttatagttac gctgggcaac
    35161 gatcatattg accagttctt tactgagatc gacgttggac gcttccagcg caccgttggt
    35221 cagagtgcca aagtttcccg tcccggctgt ccccaacagc gccacgccag aagattgcgt
    35281 cgcagaccag acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa agttcgccag
    35341 tacaatctgc cccagcagtt gggtttgttc gttggaattg ttgccgacaa ccgtaccgtc
    35401 atcattgatt tgataactca ccagatcgcc cggtttgtag ccattctggg tggttgccac
    35461 aatgttgtta gcgccggtat tttgctgcat ggagttgagg aagctcagac taaacgtggc
    35521 gggttctgca ccgttaattg cgccggttgc gatattattc gccatcgcac catccactaa
    35581 tgtgccatta gcattaaatt ccagtgttgt cgctgtcttc gcaatgctgt ttggatcact
    35641 gctatcctgg gtgtagacct gccagttatt atccccggtc ttcacaaaat agacgctcat
    35701 gtcatgagca ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc
    35761 cgcattgctg gcgctgaatg gcgtaacagt aggaagcgga tcactggaat tcaggttgat
    35821 ctgcatcgac gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatatt
    35881 ggtcggattc gccccttgct gaatagtcgg cggcgtaccg gttaccgggt aacccgtcag
    35941 ctgtaaacct tgcgtattca acaggttacg gttttcatcc agcttaaatt gtccgttacg
    36001 gctgtagaac accgaaccgt tgctgtctac cagacggaaa aaaccgttct ggctgatagc
    36061 aacgtccaga cctcgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc
    36121 ggcaactttt acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt
    36181 gcctgattta aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt
    36241 ggtggcggca gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa
    36301 tgtgaaggct taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag
    36361 atccagcgta ttaccgctgt tgccgcggat cacaccctgc accagagcaa actgcagcgg
    36421 ctgggcaacc agttgtgtac caccgttact ggcgctaatc gctacattgt aagaaccgtt
    36481 cggcgcagtg ctgccatcag tcaacgaacc gtcccaggtg aaactgtgaa ctccggcggt
    36541 cagttcacca atatcaatgg tgcgcacaac cgcgccattt ttatcggtga tggtggccgt
    36601 aactttgtct gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc
    36661 ttcttcactg ccggttcccg caagaacagt tgtgccgggg atcatcacgc cgtgaccgat
    36721 caggttactg gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt
    36781 ggtattgagt ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg acgtcagctc
    36841 gttgttttcc attggattgg tcgggtcctg gtttttcagc tgcgccacca gcaaagtcag
    36901 aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact
    36961 gacgccggta tttgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat
    37021 tgaccgagcg taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg
    37081 gcctgatagc tgcgtgacgc cgacatggtg ttaaccatct ccccgacgac atcaacgttc
    37141 ggcattttta tgtagccctt tgcatctgcc agcggattac ctggttcata aaccagtttg
    37201 tccggggcct gactttctta acatcggcaa cctttacgcc gcctgtcgca gcacctggtg
    37261 cagcgttaac ctggaatacc acctgttttg cccgatatgg ctgtccatca ggaccggtca
    37321 cgctatcagc atttgccaga ttactggccg ccacgttcag gcgctgggac tgggcagtta
    37381 acgccgaccc ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta
    37441 aaacgttcat catgcctttg atttgcccgc tcaacgcgct aaggctcatc tggtattgca
    37501 ggctgttatc ggcaaactgg gtgcgttcgc gatccatatc gacggtatta ccgtcaagcg
    37561 aaggctggtc cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc gcctgcgccg
    37621 ggatgtgctg cgttgaggtc atcgtcagtg caaccacact ggttgcatcc cgtccacgtt
    37681 gcatgacttt tttaagttca ctggcaaaat cgatatcgcg cgcctgataa ccaggggtat
    37741 cggcattggc gatgtttgct gccagcactt cctgacgctg ggcgcgcaga ttgagcgcct
    37801 cttgttgaaa acgtaaggcg gcgtcgagct tatcgagcat atctcctccg caggtatcaa
    37861 aattctgcca tcagcttaaa tgcctttacg cgcgcgttat cggcggaata aacgcaaaat
    37921 gggtcgctat ttatgccgtt gatggtcatt gcggacaggt acaattcacg ttgtagaaat
    37981 ggctgggggc gaaaatgctg acaataaaac gtagcgtggc gatcatcgcg atactgttca
    38041 gtccgttaag tacggcgagc aatctcacat cgcaattgca caactttttt agcgcccaac
    38101 tcgcgggggt aagtgatgag gttcgtgttt ctattcgtac agcgcccaat ctactaccgc
    38161 catgcgagca gccattgctt tcgatgagca ataattcccg cctgtggggc aatgtgaatg
    38221 tgttggcacg ctgcggtaac gacaaacgat atttacaggt taatgtgcag gccacaggaa
    38281 attatgtggt tgccgcgatg cctattgtgc ggggaggaaa gctggaagct ggcaatgtca
    38341 aactgaaacg tggacgcctg gataccctgc caccgcgtac ggtgctggat atcaatcaac
    38401 ttgttgatgc cgttagcctg cgcgatctat cacccgatca acctatccag ttaacccagt
    38461 ttcgccaggc atggcgggta aaagcgggac aacgcgtcaa tgtgatcgcc agcggtgatg
    38521 ggtttagcgc caacgcagaa ggtcaggcgc tgaacaatgc agccgtcgca cagaatgcgc
    38581 gggtgcgcat ggtatcggaa caggtagtca gcggcgttgt tgatgcagat gggaatattc
    38641 ttataaacct gtaagctgtt aaagattacc cgtcccttgc cgataaataa gcaacacatg
    38701 ataaaagcgc cctcaatgag gaataaacca tgagtattga tcgcacttcg cctctgaagc
    38761 ctgtaagcac cgttcaaccg cgcgaaacca ccgacgcgcc ggtaacgaac agccgggcgg
    38821 caaaaacaac cgcctccacc agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga
    38881 tgcaacccgg cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa ctggcgattc
    38941 gtaacggtga actaaaaatg gacaccggca aaattgccga tgcgctgatc aacgaagcgc
    39001 agcaagactt gcagagtaac tgatcgtatg acacgtcttg cagagatcct cgaccagatg
    39061 tccgctgtgc ttaacgatct caaaacggta atggatcaag agcagcaaca tctctctatg
    39121 gggcagatca acggcagcca gttgcaatgg attacagaac aaaaaagctc actgctggcg
    39181 acgctggatt acctcgaaca gttacgcaag aaagaaccca atacagcaaa tagcgttgat
    39241 attagtcaac gctggcagga aattactgtg aaaacgcagc aactacgcca aatgaatcaa
    39301 cataacggct ggttactgga aggacagatt gagcgcaatc aacaggcgct ggaaatgttg
    39361 aaaccgcatc aggaaccgac gctatatggg gcgaacggtc agacctcaac accccatcgc
    39421 ggcagtaaaa agatttcgat ctgaatatta tctccggcct gcactgcagg ccggaatgca
    39481 ttgttacacc gtccggcggg caaattcttt aactttgaag cccagtaccg ccagtgcagc
    39541 gaagtacgcg gcaatccccg ccagcacgac cgccattaaa cgcagtaagc gccagggcat
    39601 ggtacccaat gaccactccg gcatgatatg taacatacct aaaagcacgc cagacatcac
    39661 cagtaccgcc accaccagac gcaacagaaa cgccatccag ccgggttgcg gagtaaagat
    39721 tttctgctta cgcaactgcc agtaaagcag cgaagcattc agacacgccg ccagaccaat
    39781 agaaagtgac agcccggcat gtttcaacgg accaataaac gccaggttca tcaattgcgt
    39841 taaaatcagc gtaacgatgg caattttcac tggcgtttta atgtcctggc gggaataaaa
    39901 gccaggagcc aacactttca ctacaatcag gccaatcaaa cccaccgagt aggcaattaa
    39961 cgcccgctgg gtcatcagcg catcaaacgc ggtaaattta ccgtactgga acagcgaaac
    40021 ggttaacgga cctgaaagaa tgcccaacgc aaccgcactc ggcagtgcca acaggaaaca
    40081 aagacgcaac ccccagtcca tcaaacggtt gtattcatca tgattgccac tggcaaaact
    40141 tttcgacagc gacggcagca aaatggtgcc aagcgccacg cccagcacac cggagggaaa
    40201 ctccattaag cggtcggcgt aatacatcca agacaccgaa ccggaagcaa gaaacgaggc
    40261 aaaaatggtg ttgatgatta aggagatctg gctcacagag acgccaagga tcgccggtcc
    40321 catctgtttc accacgcgca ttgctccagc atcgtggaag ttaatgcgcg gcaggaccag
    40381 catgccgatc ttcttcaggt gcggtagctg atacaccagc tgcagaatgc cgccgaccgt
    40441 aacagcccac gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa
    40501 accaatcatg ctgatgttaa gcagtgttgg cgcaaacgcc ggaatcgaga agcggttcca
    40561 cgtattcaga atcgctccca ccagcgacgc cagggagatc agcaagatat agggaaaggt
    40621 aatctttagt agctggctgg tcagggcaaa tttgtcagct gtgtcagcga agcctggcgc
    40681 ggtcaccatg atcacccacg gtgcggcgag catgccagcg accgtcacaa ccgccagcgc
    40741 aagtgtcagc aggccagaaa cataagagac aaagacccgc gtggcgtctt caccctgctt
    40801 acttttatat tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat
    40861 acggcgtaac aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc
    40921 gccaaagatt ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg aaaacatggt
    40981 catcgagctg acggcggcca gcgattttaa taaattcatc ggtgttctaa tccggaccca
    41041 cgggcaaaac gcctgcaagg caggcgctga aagaggcgaa tagtctacct agattatggt
    41101 gaattgctac cgccagatgt tacagggttt attcactcat cgcatcgcgc cagatcttgt
    41161 caacgatacg ttgcgccagc actgcctatt cgccggcggt ttgcggaact gtctgatttt
    41221 acacgcattc aatgaagtgc cgcgcacagc cgacaaatcc acgttgctca agagtgctct
    41281 gccaaccagg aatcggttta ttcactacac cctgcccgcg ctcctcacgc cattcgcgca
    41341 tatccgtaat gtcgatgagc gcaccgtcag tcacggcctg cacggtttca cgctgacttc
    41401 cggcacggcg atgcatacaa gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg
    41461 caaacagcat ttcgccagcg tcgttagtca gtagcgtacc gccatccaga gaggctttgc
    41521 cgcccgacaa ccacagcgcg gtatccacca catgcagata atcatccagc aacgtgaaat
    41581 aaagatcgtg tggcccgacg ctattgctac gatgtttatc cattcttagc gaggctgcgg
    41641 tggcgagttg cgtttttaac tcaccgtaga gtggtgcgaa acgacggtta aaaccgacca
    41701 tcaacgtcag ttttttccgc gccgccagtt ccaccagcct ttcagcatcg cgcagatttt
    41761 ctgccagcgg tttatcgaca cagacatgta cccccgcatt gagtaacgta ctgaccacgt
    41821 caaagtggct ggcggtgctg gagtgcacaa aaaccgcatc acaactggcg gcaaggctgg
    41881 ataacgaatc ggcataagga atgcgccagc tgtcacaaat tggcagggct ttggcgcgcg
    41941 taggcgacca ggctccttgt aacgtccagt cagacgctgc cgccagcacc ggtaaccacg
    42001 ctttttgcac aatgccgcct aatcccacta cgccgatacg taattttttc actttaatct
    42061 cccagatggg ccagcaacga atcaagacgt tgtttcagtt ctgccacttc gatttccagg
    42121 gcttcgacgc gggtctgtaa atcaccgtca accatgttcg acatagccgt taccgccggc
    42181 tgatcttcaa cctcaccact gaaaagatgc atgtagcggc tttcgcgttt acccggttcg
    42241 cgggccagac gcaccacaaa aggaccatct tcgcgattcg ccagttgttc cagcgtcgac
    42301 tccacttccg ccatatcgct gaattcatac attcgcgcgg cgcggctgcg cagttcaccc
    42361 ggcgtctggg caccacgcaa taacaacgtg gtgattaacg ccacttctgc ggcgctcagt
    42421 ttcagatcgc caaattctga attacaaaaa cgttgctcat atttggtgac ccgattacca
    42481 aaaccgctca ctgtgcgtag ataatgacgt ttgaccagat tatccagctg ttcctgcact
    42541 tcggattcac tcagattcat gaccggttca cggttcgttt tctgattaca ggccgtgact
    42601 acaccattga ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa
    42661 ccaattacgc gagcttccag ggcagtaagt tgatatttca tgagattctc cttagcggcc
    42721 gggcgtccag tctggggtag ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat
    42781 caacagatag tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc
    42841 gctgcgttta ttgtgcggca tataattagc cataatgcga tgaatatgtt gggtgcgctg
    42901 catataacga atggctgcgg tcagggcttc aaacatgagt cctttgccct gccatttttg
    42961 cccaatcgaa taaccgagat agcaggcatg aaaagagccg cgaacaacat tggaaaaatt
    43021 ggcaacgcca ataatctctt tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc
    43081 ttgtttatga aattcgttaa tcatccctag cctggcctgc cagcctgatg gataacagtg
    43141 gctttcgtcg cgcactggct cccagggctt gaggaaatgg cgattctctg cgtaataatc
    43201 cgccagacgc caggcatcac gatcatgcac cagacgcacg accagtcggt ctgtggttaa
    43261 gcgcactttt ggcacgttac tgcgatagcc aaacattcta tacctactcc ttcccgtaac
    43321 gtctttaata cgctgtaagt attactatac ctgcgcaacc agaccctgtg aaaagtccaa
    43381 aatgccattt tctgcattat tcacatattt gataaaaact ctctatcaaa gcgacgtttt
    43441 gataaaaaaa tactgtcccc gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa
    43501 agtgctcatc ttttatttcc cgggagggga aatgtcccgc gtatcgcagg cgaggaacct
    43561 gggtaaatat ttcctgctca tcgataatat gctggtcgtg ctggggttct ttgttgtctt
    43621 cccgctgatc tctatccgct tcgttgatca aatgggctgg gccgccgtca tggtcggtat
    43681 tgctctcggt ctacgccagt ttattcagca aggtctgggt attttcggcg gcgcaattgc
    43741 cgaccgcttt ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg ccgccggatt
    43801 cgccacaatg ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc tgctctcggg
    43861 actcggcggc acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac taatccgtcc
    43921 acaacaacgt ggtcgttttt tctcgctgtt gatgatgcag gacagtgcca gtgcggtcat
    43981 tggcgcattg ttggggagct ggctgttgca atacgacttt cgcctggtct gcgccacagg
    44041 ggccgttttg tttgtgctat gtgcggcgtt caatgcgtgg ttgttaccgg catggaaact
    44101 ctccaccgta cgcacgcccg ttcgcgaagg catgacccgc gtgatgcgtg acaagcgttt
    44161 tgtcacctat gtcctgacgc tggcgggtta ctacatgctg gctgtacaag tgatgctgat
    44221 gctgccaatt atggtcaacg acgtggctgg cgcgccctct gccgttaaat ggatgtatgc
    44281 cattgaagcg tgtctgtcgt taacgttgct ctaccctatc gcccgctgga gtgaaaagca
    44341 ttttcgtctg gaacaccggt tgatggctgg gctgttgata atgtcattaa gcatgatgcc
    44401 ggtgggcatg gtcagcggcc tacaacaact tttcaccctg atttgtctgt tttatatcgg
    44461 gtcgatcatt gccgagcctg cgcgtgaaac cttaagtgct tcgctggcgg acgcaagagc
    44521 tcgcggcagc tatatggggt ttagccgtct gggtctggcg attggcggcg ctattggtta
    44581 tatcggtggt ggctggctgt ttgacctggg caaatcggcg caccagccag agcttccgtg
    44641 gatgatgctg ggaattattg gcatcttcac tttccttgcg ctgggttggc agtttagtca
    44701 gaaacgcgcc gcgcgtcgtt tgctggaacg cgacgcctga tttgatactc ccccctgact
    44761 cctccatact gaaagtgaac tgacagccaa atggaggagt cacgtgaagc tatacattta
    44821 cgatcactgc ccttactgcc tcaaagcccg catgattttc ggcctgaaaa atatccccgt
    44881 cgaattacat gttctgctca acgacgacgc agaaacaccc acccggatgg tcggtcaaaa
    44941 acaggttccc attctgcaaa aagatgacag ccgctatatg ccagaaagca tggatatcgt
    45001 tcactatgtc gataaactcg acggcaaacc gttactgacc ggcaaacgtt cccctgccat
    45061 tgaagagtgg ctgcgcaagg tcaatggcta cgtcaacaaa ctgctgttgc cgcgttttgc
    45121 caaatcggca tttgatgagt tttctactcc cgccgcgcgc aaatatttcg tcgacaagaa
    45181 agaggccagc gcgggtaatt ttgccgacct gctggcccac tctgacggtc tgattaagaa
    45241 tatcagcgat gatttgcgtg cgctggacaa actgatcgtc aaaccgaacg ccgtgaatgg
    45301 cgaactttcg gaagatgata ttcagctatt tccgctactg cgtaatctga cgctggtagc
    45361 cggaatcaac tggccaagcc gcgttgctaa ttaccgcgat aatatggcga aacagacaca
    45421 aatcaatttg ttatcatcaa tggcgattta atcctgaccc gccgcggcag cctgtattcg
    45481 cgggttgccg ccgttttcag ttttttctgc tggcgagttt atcgctggtg acacatgctg
    45541 cgttgtgtca ggatattgac gcagatttca tgtcgaggac taccatgaac aagtttttat
    45601 ttgccgctgc attgatcgtc agtggcctgc tcgttggctg taatcaactc acccaataca
    45661 ccatcaccga acaagaaatt aaccagtcgc ttgcgaaaca taataatttc tcaaaagata
    45721 tcggtttacc cggcgtggct gacgcccata ttgttctgac aaacttgacc agccaaattg
    45781 gtcgcgaaga gccgaataag gttaccctaa ccggagacgc caatctggac atgaactccc
    45841 tgttcggtag tcagaaagcg accatgaaac tgaagctgaa agcgctgccc gtgtttgata
    45901 aagaaaaagg tgcgatcttc ctgaaagaga tggaagtggt cgatgcgacg gtacaaccgg
    45961 aaaaaatgca aacggtgatg caaacgttgc ttccctattt gaaccaggca ttacgcaatt
    46021 actttaacca gcaacctgct tacgtcctgc gcgaagatgg cagtcagggc gaagcaatgg
    46081 cgaaaaaact ggcgaaaggc attgaagtga agccaggcga aattgtcatt ccgtttacgg
    46141 attaatgacg agggcgcatt cgcgcccttt atttttcgtg caaaggaaaa cgtttccgct
    46201 tatcctttgt gtccggcaaa aacatccctt cagccggagc atagagatta atgactgcac
    46261 catcccaggt attaaagatc cgccgcccag acgactggca ccttcacctc cgcgatggcg
    46321 acatgttaaa aactgtcgtg ccgtatacca gcgaaattta tggacgggct atcgtaatgc
    46381 ccaatctggc tccgcccgtg accacagttg aggctgccgt ggcgtatcgc caacgtattc
    46441 ttgacgccgt acctgccggg cacgatttca ccccattgat gacctgttat ttaacagatt
    46501 cgctggatcc taatgagctg gagcgcggat ttaatgaagc cgtgttcacc gctgcaaaac
    46561 tttacccggc aaacgcaacc actaactcca gccacggcgt gacgtcaatt gacgcaatca
    46621 tgccggtact tgagcgcatg gaaaaaatcg gtatgccgct actggtgcat ggtgaagtga
    46681 cacatgcaga tatcgacatt tttgatcgtg aagcgcgctt tatagaaagc gtgatggaac
    46741 ctctgcgcca gcgcctgact gcgctgaaag tcgtttttga gcacatcacc accaaagatg
    46801 ctgccgacta tgtccgtgac ggaaatgaac ggctggctgc caccatcact ccgcagcatc
    46861 tgatgtttaa ccgcaaccat atgctggttg gtggcgtgcg tccgcacctg tattgtttac
    46921 ccatcctcaa acgcaatatt caccaacagg cattgcgtga actggtcgcc agcggtttta
    46981 atcgagtatt cctcggtacg gattctgcgc cacatgcacg tcatcgcaaa gagagcagtt
    47041 gcggctgcgc gggctgcttc aacgccccaa ccgcgctggg cagttacgct accgtctttg
    47101 aagagatgaa tgctctgcag cactttgaag cattctgttc tgtaaacggc ccgcagttct
    47161 atggcttgcc ggtcaacgac acattcatcg aactggtacg tgaagagcaa caggttgctg
    47221 aaagcatcgc actgactgat gacaccctgg tgccattcct cgccggggaa acggtacgct
    47281 ggtccgttaa acaataaaaa tcgtagcttc ctgttgtcat taggttattt tacctgtata
    47341 aataaccagt atattcaaca gggggctatt atgcgaattg aagtcaccat agcgaaaact
    47401 tctccattgc cagctggggc tattgacgcc ctggctggcg aactttcccg ccgtattcag
    47461 tatgcgtttc ctgataatga aggccacgta tcggtacgtt atgccgcagc gaataattta
    47521 tcggttattg gcgcaacaaa agaagataaa cagcgcatta gcgaaattct ccaggaaacg
    47581 tgggaaagcg ccgatgactg gtttgtcagc gaataatatg cagtgatttt tgtttttgcc
    47641 ggatcgcccc ggctttttta ttttacttac gaattttgag tacaaaattt ctacacttcc
    47701 tcaaaataat tgcacaatat gctaaaaaaa tatgaatgac atgctgtttt attgttcata
    47761 taaatcttaa aagcagaaat ttatctgttc ccccctttaa aaattcggtg aataccctta
    47821 cttattggta tactgaagtt gcttcagata aaaacgcatt gaacctcgaa taacgttgtc
    47881 tagtaacacg aattaggggg ccatgatgga aaaaaataat gaagtcattc agactcatcc
    47941 gctcgtaggg tgggacatca gcaccgttga tagctatgat gcgctgatgt tgcgtttgca
    48001 ctaccagacc ccaaataagt ccgagcagga agggactgaa gttggtcaga cgctctggtt
    48061 aaccactgat gttgccagac aatttatttc gatattagaa gcaggaatcg ccaaaattga
    48121 atccggtgat ttcccggtaa acgagtatcg gcgtcattaa catgacatga ccatccaccc
    48181 aaaaggcacc tcaccggtgc ctttaccatt tctgggttgt atatctgcat tcagttaatt
    48241 actctgctta aaccatgaga gcagagagaa aaaaatgaaa tacgatctca tcattattgg
    48301 cagcggttcc gtaggcgctg ccgccgggta ttatgcaacc cgcgccggtt taaacgtgct
    48361 aatgaccgac gcccatatgc caccgcatca acacggcagc caccacggcg atacgcgatt
    48421 aattcgccat gcttatggtg aaggcgaaaa gtatgtcccg ttggtcctcc gcgcgcaaac
    48481 gctgtgggat gaactttccc gccacaacga agatgatccc atttttgtac gctctggtgt
    48541 cattaacctc ggcccggctg actccgcatt tctcgccaac gtcgcccaca gcgccgaaca
    48601 gtggcaactc aacgttgaaa agctcgatgc gcaagggatt atggcccgct ggccagaaat
    48661 acgcgtcccg gacaactaca tcggcttatt tgagactgat tccggttttt tgcgcagcga
    48721 actggcgatt aaaacctgga tccaactggc gaaggaagcg ggctgtgcgc aactgttcaa
    48781 ctgcccggtc accgcaattc gtcatgacga tgatggcgta actattgaaa cggttgacgg
    48841 tgagtatcag gcgaaaaaag cgattgtctg cgcgggaaca tgggtaaaag acctgctccc
    48901 ggagctgcct gtccagcccg tacgtaaagt atttgcctgg tatcaggccg atggccgcta
    48961 tagcgtgaag aataaattcc cggcgtttac cggtgaactg cccaatggcg atcaatatta
    49021 tggttttccg gcagaaaacg acgcgttgaa gattggcaaa cataacggag gccaggttat
    49081 ccattcagcg gatgaacgtg ttccgtttgc ggaagtggcc agtgatgggt cagaagcctt
    49141 cccgttcttg cgcaatgtat tgccgggtat cggttgctgc ctgtacggcg ctgcctgcac
    49201 ctatgataat tcgcctgacg aagattttat tatcgatacc ctacccggcc acgataatac
    49261 actgctcatt accggcctga gtgggcacgg ttttaaattt gcgtcagttt taggggaaat
    49321 agctgccgat tttgcgcaag accaaaaaag cgattttgat ttgacgccat tcaggctttc
    49381 ccgcttccaa taatcatcat tacggcctcc agaatgaggc cgtttttaca ggagtattta
    49441 atgcgtcggc tgttgcacta tctcatcaat aatattcgcg agcatctgat gctttatctt
    49501 tttctctggg gattgttggc aattatggat ctgatctatg tgttttattt ttagttttcc
    49561 gcactgacat aaactgacaa atatttaatc tccctttcat ttagcgtgaa aaattgaatg
    49621 agattattcc tgcttctaca gcactcacca gacatctctg atgctgaacc cactaaactt
    49681 atgaagattc tttcattatt aattcaaaat tattgaaata aaaataaata tcattcaatt
    49741 tgttattttt gtgttgcaat tcattctccg acacgccatt ttctttctct cgcaaattcg
    49801 tattggatgc acttatgtca ttcacaaata cccctgaacg ctatggtgtt atatctgcgg
    49861 ccttccattg gctaagcgca attattgtct acggcatgtt tgctttaggt ctgtggatgg
    49921 tcacgctcag ttattacgat ggctggtatc acaaagcacc cgaactgcat aaaagcatcg
    49981 gtattttgtt aatgatgggg ctagttattc gcgttctgtg gcgtgtcata tctccaccgc
    50041 cgggtccgct gccgagttat tcgcccatga ctcgtcttgc cgcaaaagct ggacatctcg
    50101 cattgtgcct attgctgttc gccatcggca tcagcggcta tctgatctca actgccgatg
    50161 gtaaaccgat cagcgttttt ggctggtttg acgtccccgc gacccttgcc gacgctggcg
    50221 cacaggcaga ctttgccggt gccctgcatt tttggctcgc gtggagcgtc gtagtactgt
    50281 ctgttatgca cggatttatg gccctgaagc atcatttcat cgataaagac gacactctga
    50341 agcgcatgct gggaaagtcg tcatctgact atggagtata aaaaatgaaa aaaagcctgc
    50401 ttggtttaac cttcgcgtcc ctgatgttct ctgccggttc agcggttgcc gccgattaca
    50461 aaattgacaa agaaggtcag cacgcctttg ttaacttccg catacagcat cttggctata
    50521 gctggttata tggcaccttt aaagatttcg acggtacttt tacctttgat gaaaaaaatc
    50581 cggctgccga taaagtgaat gtgacaatta acaccaccag cgtcgatact aaccacgccg
    50641 aacgcgataa acatcttcgc agtgcggatt ttctcaatac cacaaaatat ccacaggcaa
    50701 cattcacctc caccagcgtg aagaaagacg gtgacgaact ggatattacc ggcgatctga
    50761 cgctgaatgg cgtaaccaaa cccgtcacgc tggaagcgaa attaattggt cagggcgacg
    50821 acccatgggg cggtaaacgt gcaggctttg aggccgaagg caaaattaag ctcaaagact
    50881 tcaatatcaa gacagattta ggtccagctt ctcaggaagt ggatctgatt atttcagtgg
    50941 aaggcgtaca gcagaagtaa gagtgaaatg ttgccggatg gcacgcatcc ggcaatgata
    51001 tttattctgt tggatcagga atacccagtg ttgtattcag acgtccacga gacttattaa
    51061 agatcttatt gccattttca cgtcctgccc gacggcgtcg ctgttcttct ggcggtaacg
    51121 cgctttcttc gcagcaaatc tcactacaac aacctttgta tttttccgcg catactggac
    51181 actgaataaa cagcaggtgg cagccatcat ttttacagtt ggtatggctg tcgcacggcg
    51241 cgccgcactg gtggcaatgc gcgataatct catccgatat acgttcgccc atccgctcgt
    51301 caaaaacaaa atttttgcca ataaaacgca ccggcaagcc ctgctcgcgc gccttacggg
    51361 cgtattcaat aattccgccc tcgatatgcc agactttgtt gaatccgtta tgtttcatcc
    51421 aggcactggc cttttcacaa cgaatgccgc cggtgcagta catgacgatt tttttatctt
    51481 tatgtgcctg catcatctcg actgcttttg gcagctgctc acggaaggta tctgccggaa
    51541 tttcgagcgc gttttcaaag tgccccactt catactcata gtggttacgc atgtcgataa
    51601 atagtgcatc gggatcgtca agcatggcgt tcacttccgc cgcttgcaga tactcaccaa
    51661 cattgctggc atcaaagtga ggatcgtcaa taccgtcggc aacaatgcga tcgcgtacct
    51721 tcatgcgcag tacccagaag gatttcccgt catcatccaa cgcgatattc aggcgtaaac
    51781 cctctaaagc cgggtcgaag gcatagagct gcgcgcgaaa tgtttcaaca ttgctcgcag
    51841 gtacgctgat ttgcgcgtta atgccctcat gcgccagata cactcgccca aaaacattca
    51901 gcgcggtaaa cagctgatat aaagcgtcac gggtcgcctt aggatcggcg atgtggaaat
    51961 acttgtaaaa cgaaatggtg gttcgcggtt cgctctcagc caacattttg gcttttaacg
    52021 cgtcgttgga aatgcggttg tgtaacactg gcatggtgta cggttcctgc gagatgggaa
    52081 agtaaaaatc cgcggcatga tatagcaatt atcgataatt aacatccaca tattttacgc
    52141 tacatttgcg cattaaaaat tatttgttat ttacaagcgc ggcaatttcg cccagtcttc
    52201 agccacaaat tttggttgcg ggcgaaaaaa tgcgacaata catacaattg cccgtatagg
    52261 ttgaaaaaca ggattgatat gacgaatcta cccaagttct ccaccgcact gcttcatccg
    52321 cgttattggt taacctggtt gggtattggc gtactttggt tggtcgtgca attgccctac
    52381 ccgattatct accgcctcgg ttgtggatta ggaaaactgg cgttacgttt tatgaaacga
    52441 cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag cgaacaagaa
    52501 cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca tgggcctgat ggaaaccggt
    52561 atggcgtggt tctggccgga ccgccgaatc gcccgctgga cggaagtgat cggcatggaa
    52621 cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat ccattttctg
    52681 acactggagc tgggcgcgcg gcagtttggt atgcaggaac cggggattgg cgtttaccgc
    52741 ccgaacgata atccactgat tgactggcta caaacctggg gccgtttgcg ctcaaataaa
    52801 tcgatgctcg accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa aggcgaagtg
    52861 gtctggtacg caccagatca tgattacggc ccgcgctcaa gcgttttcgt cccgttattt
    52921 gccgttgagc aggctgcgac cacgaccgga acctggatgc tggcacggat gtccggcgca
    52981 tgtctggtgc ccttcgttcc acgccgtaag ccagatggca aagggtatca attgattatg
    53041 ctgccaccag agtgttctcc gccactggat gatgccgaaa ctaccgccgc gtggatgaac
    53101 aaagtggtcg aaaaatgcat catgatggca ccagagcagt atatgtggtt acaccgtcgc
    53161 tttaaaacac gcccggaagg cgttccttca cgctattaaa tctcccatgc cggatgcttc
    53221 agaatggcat ccggcattac cacagcaaat ccccctgatt tagcgataaa agctctctgg
    53281 attgcgcccc ctggaagtcg ggcgcataat tagtgtgttt atcttttctt cttatgttca
    53341 ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct gtgaaaatga cacccctata
    53401 aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg ggccgccttc
    53461 agtctggtaa tgcccttctt acccctctac gttgagcagc ttggtgttac cggccactcc
    53521 gccctgaata tgtggtccgg tattgtcttc agtataatgg gttcctaaac acatcatttt
    53581 aggtattgaa tatgagacgg gcaatcagct acatcagatt ttcaagtgaa cgacaactga
    53641 aaggggactc tgtacgccga caatctaagc tggttactga ctggcttgat aagaaccctg
    53701 agttttactt ggattcatcc ttgagcttta aagatttggg gaaaagtgct tttagtggta
    53761 agcacctgaa aggtggtcta ggtgactttt taaccgctat cgagaaaggg cttgttaaag
    53821 ctggtgatac gctactaatc gagagtttag accgtctgtc acgtcaggat attgatattg
    53881 cttctgagct attacgccgt atcttaaggg ctggtgtaga tgtagtcaca ctgagcgatg
    53941 gagaacatta cacacgagaa agtttaaaag acccgttagc cctgattaaa agcattctga
    54001 taatgcagcg ggcgcatgag gaaagtttga gaaagtctga gcgtgtccaa gctgcatgga
    54061 accgtaaaaa agaactcatc tccgaaggta tcaaggtatc taggcgttgt cctgcatggt
    54121 taaggctgaa tgatgaccgt cgaacattca caatcattcc tgataaagtg gaagttgtta
    54181 aacgagcatt tgacctgaga ttacagggtt taagcttttg ggctatcacc agaactttaa
    54241 acgatgaagg tcacttgtca ttgaatcaat acacacccaa acaaaagggg tggtctgata
    54301 ctgctgtaaa gaaactgctc agaaatcgtg ctgtgattgg atgtttcaca cctgctggac
    54361 gtgaagaagt tcagggttac taccccgcaa taatcagtga gtcactattc tatcgagtac
    54421 agcaactgaa cacagggcaa tatggtaggg cttctgtatc aagtaatccg ctatcagtca
    54481 acttgttcag gggtatcatc aa
//
LOCUS       DAZSNO010000010        53211 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZSNO010000010 DAZSNO010000000
VERSION     DAZSNO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53211
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.010"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(11..322)
                     /locus_tag="ACHWN2_000548"
     CDS             complement(11..322)
                     /locus_tag="ACHWN2_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR8930216.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="ACHWN2_000549"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHWN2_000549"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="ACHWN2_000550"
     CDS             701..1054
                     /locus_tag="ACHWN2_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR8930217.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHWN2_000551"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHWN2_000551"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFR8930218.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="ACHWN2_000552"
     CDS             complement(2810..3280)
                     /locus_tag="ACHWN2_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR8930219.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHWN2_000553"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHWN2_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /locus_tag="ACHWN2_000554"
     CDS             complement(4119..4337)
                     /locus_tag="ACHWN2_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR8930220.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHWN2_000555"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHWN2_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR8930221.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /gene="acrB"
                     /locus_tag="ACHWN2_000556"
     CDS             complement(5283..8432)
                     /gene="acrB"
                     /locus_tag="ACHWN2_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR8930222.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="ACHWN2_000557"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="ACHWN2_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR8930223.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="ACHWN2_000558"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="ACHWN2_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR8930224.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="ACHWN2_000559"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="ACHWN2_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR8930225.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="ACHWN2_000560"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="ACHWN2_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR8930226.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="ACHWN2_000561"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="ACHWN2_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR8930227.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="ACHWN2_000562"
     CDS             14911..15288
                     /locus_tag="ACHWN2_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR8930228.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="ACHWN2_000563"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="ACHWN2_000563"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR8930229.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="ACHWN2_000564"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="ACHWN2_000564"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR8930230.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="ACHWN2_000564"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="ACHWN2_000565"
     CDS             18099..18428
                     /locus_tag="ACHWN2_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR8930231.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="ACHWN2_000566"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="ACHWN2_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR8930232.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="ACHWN2_000567"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="ACHWN2_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR8930233.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="ACHWN2_000568"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="ACHWN2_000568"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR8930234.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="ACHWN2_000569"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="ACHWN2_000569"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR8930235.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="ACHWN2_000570"
                     /pseudo
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="ACHWN2_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="ACHWN2_000571"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="ACHWN2_000571"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR8930236.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /gene="ybaL"
                     /locus_tag="ACHWN2_000572"
     CDS             complement(25481..27157)
                     /gene="ybaL"
                     /locus_tag="ACHWN2_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR8930237.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="ACHWN2_000573"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="ACHWN2_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR8930238.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="ACHWN2_000574"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="ACHWN2_000574"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR8930239.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="ACHWN2_000575"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="ACHWN2_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR8930240.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="ACHWN2_000576"
     CDS             complement(31205..31999)
                     /locus_tag="ACHWN2_000576"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR8930241.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="ACHWN2_000577"
     CDS             32137..32478
                     /locus_tag="ACHWN2_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR8930242.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="ACHWN2_000578"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="ACHWN2_000578"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR8930243.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="ACHWN2_000579"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="ACHWN2_000579"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR8930244.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="ACHWN2_000580"
     CDS             36394..37686
                     /locus_tag="ACHWN2_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR8930245.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37717..37814
                     /locus_tag="ACHWN2_000581"
                     /pseudo
     CDS             37717..37814
                     /locus_tag="ACHWN2_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37811..38218
                     /gene="cueR"
                     /locus_tag="ACHWN2_000582"
     CDS             37811..38218
                     /gene="cueR"
                     /locus_tag="ACHWN2_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR8930246.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="ACHWN2_000583"
     CDS             complement(38219..38677)
                     /locus_tag="ACHWN2_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR8930247.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="ACHWN2_000584"
     CDS             complement(38674..39591)
                     /locus_tag="ACHWN2_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR8930248.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="ACHWN2_000585"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="ACHWN2_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR8930249.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="ACHWN2_000586"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="ACHWN2_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR8930250.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="ACHWN2_000587"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="ACHWN2_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR8930251.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="ACHWN2_000588"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="ACHWN2_000588"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR8930252.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="ACHWN2_000589"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="ACHWN2_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /gene="ybbA"
                     /locus_tag="ACHWN2_000590"
     CDS             43550..44236
                     /gene="ybbA"
                     /locus_tag="ACHWN2_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR8930253.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /gene="ybbP"
                     /locus_tag="ACHWN2_000591"
                     /pseudo
     CDS             44233..46648
                     /gene="ybbP"
                     /locus_tag="ACHWN2_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(46866..47021)
                     /gene="dinQ"
                     /locus_tag="ACHWN2_000592"
     CDS             complement(46866..47021)
                     /gene="dinQ"
                     /locus_tag="ACHWN2_000592"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR8930254.1"
                     /translation="MKKIIVIGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="ACHWN2_000593"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="ACHWN2_000593"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR8930255.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="ACHWN2_000594"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="ACHWN2_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFR8930256.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="ACHWN2_000595"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="ACHWN2_000595"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFR8930257.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="ACHWN2_000596"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="ACHWN2_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFR8930258.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="ACHWN2_000597"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="ACHWN2_000597"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFR8930259.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53211
                     /locus_tag="ACHWN2_000598"
     CDS             52939..>53211
                     /locus_tag="ACHWN2_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR8930260.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12831 a        13691 c        14016 g        12673 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcacagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacctgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctagtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgttgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tatggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgactat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct atggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcggtctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg tttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggagg cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 cggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagtttta
    49561 gaaaaacatt ccaacttttg tattaatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc actcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt
    51121 ccaactaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct ggggggttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat t
//
LOCUS       DAZSNO010000011        52844 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZSNO010000011 DAZSNO010000000
VERSION     DAZSNO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52844
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.011"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1100)
                     /locus_tag="ACHWN2_000599"
     CDS             complement(<1..1100)
                     /locus_tag="ACHWN2_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR8930261.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="ACHWN2_000600"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="ACHWN2_000600"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR8930262.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="ACHWN2_000601"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="ACHWN2_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR8930263.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="ACHWN2_000602"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="ACHWN2_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR8930264.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="ACHWN2_000603"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="ACHWN2_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR8930265.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="ACHWN2_000604"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="ACHWN2_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR8930266.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="ACHWN2_000605"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="ACHWN2_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR8930267.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="ACHWN2_000606"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="ACHWN2_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFR8930268.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="ACHWN2_000607"
     CDS             complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="ACHWN2_000607"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR8930269.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="ACHWN2_000608"
     CDS             complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="ACHWN2_000608"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR8930270.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="ACHWN2_000609"
     CDS             complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="ACHWN2_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR8930271.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="ACHWN2_000610"
     CDS             complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="ACHWN2_000610"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR8930272.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10283..11701
                     /gene="puuA"
                     /locus_tag="ACHWN2_000611"
     CDS             10283..11701
                     /gene="puuA"
                     /locus_tag="ACHWN2_000611"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR8930273.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11850..12014
                     /locus_tag="ACHWN2_000612"
     CDS             11850..12014
                     /locus_tag="ACHWN2_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930274.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12004..13389
                     /gene="puuP"
                     /locus_tag="ACHWN2_000613"
     CDS             12004..13389
                     /gene="puuP"
                     /locus_tag="ACHWN2_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR8930275.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13523..13768
                     /locus_tag="ACHWN2_000614"
     CDS             13523..13768
                     /locus_tag="ACHWN2_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR8930276.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14081..15724
                     /gene="sapA"
                     /locus_tag="ACHWN2_000615"
     CDS             14081..15724
                     /gene="sapA"
                     /locus_tag="ACHWN2_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR8930277.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15721..16686
                     /gene="sapB"
                     /locus_tag="ACHWN2_000616"
     CDS             15721..16686
                     /gene="sapB"
                     /locus_tag="ACHWN2_000616"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR8930278.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16673..17563
                     /gene="sapC"
                     /locus_tag="ACHWN2_000617"
     CDS             16673..17563
                     /gene="sapC"
                     /locus_tag="ACHWN2_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR8930279.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17563..18555
                     /gene="sapD"
                     /locus_tag="ACHWN2_000618"
     CDS             17563..18555
                     /gene="sapD"
                     /locus_tag="ACHWN2_000618"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR8930280.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18557..19363
                     /gene="sapF"
                     /locus_tag="ACHWN2_000619"
     CDS             18557..19363
                     /gene="sapF"
                     /locus_tag="ACHWN2_000619"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR8930281.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19431..19784
                     /locus_tag="ACHWN2_000620"
     CDS             19431..19784
                     /locus_tag="ACHWN2_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR8930282.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20152..20940
                     /gene="fabI"
                     /locus_tag="ACHWN2_000621"
     CDS             20152..20940
                     /gene="fabI"
                     /locus_tag="ACHWN2_000621"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR8930283.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTRESFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21084..22211
                     /locus_tag="ACHWN2_000622"
     CDS             21084..22211
                     /locus_tag="ACHWN2_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFR8930284.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22279..24213
                     /gene="rnb"
                     /locus_tag="ACHWN2_000623"
     CDS             22279..24213
                     /gene="rnb"
                     /locus_tag="ACHWN2_000623"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR8930285.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24291..24394)
                     /locus_tag="ACHWN2_000624"
                     /pseudo
     CDS             complement(24291..24394)
                     /locus_tag="ACHWN2_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24448..26422
                     /gene="pdeR"
                     /locus_tag="ACHWN2_000625"
                     /pseudo
     CDS             24448..26422
                     /gene="pdeR"
                     /locus_tag="ACHWN2_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(26404..26532)
                     /gene="ymiD"
                     /locus_tag="ACHWN2_000626"
     CDS             complement(26404..26532)
                     /gene="ymiD"
                     /locus_tag="ACHWN2_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR8930286.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            26570..26743
                     /gene="yciZ"
                     /locus_tag="ACHWN2_000627"
     CDS             26570..26743
                     /gene="yciZ"
                     /locus_tag="ACHWN2_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR8930287.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26833..27582
                     /locus_tag="ACHWN2_000628"
     CDS             26833..27582
                     /locus_tag="ACHWN2_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR8930288.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27851..28069
                     /gene="osmB"
                     /locus_tag="ACHWN2_000629"
     CDS             27851..28069
                     /gene="osmB"
                     /locus_tag="ACHWN2_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR8930289.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="ACHWN2_000630"
     CDS             complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="ACHWN2_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR8930290.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="ACHWN2_000631"
     CDS             complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="ACHWN2_000631"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR8930291.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="ACHWN2_000632"
     CDS             complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="ACHWN2_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR8930292.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="ACHWN2_000633"
     CDS             complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="ACHWN2_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR8930293.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="ACHWN2_000634"
     CDS             complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="ACHWN2_000634"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR8930294.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32018..32608
                     /gene="ribA"
                     /locus_tag="ACHWN2_000635"
     CDS             32018..32608
                     /gene="ribA"
                     /locus_tag="ACHWN2_000635"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR8930295.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="ACHWN2_000636"
     CDS             complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="ACHWN2_000636"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR8930296.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35511..35606)
                     /gene="ymiC"
                     /locus_tag="ACHWN2_000637"
     CDS             complement(35511..35606)
                     /gene="ymiC"
                     /locus_tag="ACHWN2_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR8930297.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35720..35887)
                     /gene="yciX"
                     /locus_tag="ACHWN2_000638"
     CDS             complement(35720..35887)
                     /gene="yciX"
                     /locus_tag="ACHWN2_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR8930298.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35890..36030)
                     /locus_tag="ACHWN2_000639"
     CDS             complement(35890..36030)
                     /locus_tag="ACHWN2_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR8930299.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="ACHWN2_000640"
     CDS             complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="ACHWN2_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR8930300.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37533..40130)
                     /gene="topA"
                     /locus_tag="ACHWN2_000641"
     CDS             complement(37533..40130)
                     /gene="topA"
                     /locus_tag="ACHWN2_000641"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR8930301.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40510..40761
                     /locus_tag="ACHWN2_000642"
     CDS             40510..40761
                     /locus_tag="ACHWN2_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR8930302.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="ACHWN2_000643"
     CDS             complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="ACHWN2_000643"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR8930303.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42066..42824
                     /locus_tag="ACHWN2_000644"
     CDS             42066..42824
                     /locus_tag="ACHWN2_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR8930304.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42821..43411
                     /gene="cobO"
                     /locus_tag="ACHWN2_000645"
     CDS             42821..43411
                     /gene="cobO"
                     /locus_tag="ACHWN2_000645"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR8930305.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="ACHWN2_000646"
     CDS             complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="ACHWN2_000646"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR8930306.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44424..45044)
                     /locus_tag="ACHWN2_000647"
     CDS             complement(44424..45044)
                     /locus_tag="ACHWN2_000647"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR8930307.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45041..45922)
                     /gene="rnm"
                     /locus_tag="ACHWN2_000648"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(45041..45922)
                     /gene="rnm"
                     /locus_tag="ACHWN2_000648"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR8930308.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46060..46104
                     /gene="trpL"
                     /locus_tag="ACHWN2_000649"
     CDS             46060..46104
                     /gene="trpL"
                     /locus_tag="ACHWN2_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR8930309.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46081..46174
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46196..47758
                     /gene="trpE"
                     /locus_tag="ACHWN2_000650"
     CDS             46196..47758
                     /gene="trpE"
                     /locus_tag="ACHWN2_000650"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR8930310.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47758..49353
                     /gene="trpD"
                     /locus_tag="ACHWN2_000651"
     CDS             47758..49353
                     /gene="trpD"
                     /locus_tag="ACHWN2_000651"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR8930311.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49354..50715
                     /gene="trpCF"
                     /locus_tag="ACHWN2_000652"
     CDS             49354..50715
                     /gene="trpCF"
                     /locus_tag="ACHWN2_000652"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR8930312.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50727..51920
                     /gene="trpB"
                     /locus_tag="ACHWN2_000653"
     CDS             50727..51920
                     /gene="trpB"
                     /locus_tag="ACHWN2_000653"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR8930313.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51920..52726
                     /gene="trpA"
                     /locus_tag="ACHWN2_000654"
     CDS             51920..52726
                     /gene="trpA"
                     /locus_tag="ACHWN2_000654"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR8930314.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT        12541 a        13825 c        13324 g        13154 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgctctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt acccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgcg cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
     8281 gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt agccggagaa
     8341 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
     8401 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
     8461 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcggttttca
     8521 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
     8581 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
     8641 gctgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc attgcgctta
     8701 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     8761 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     8821 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     8881 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8941 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     9001 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     9061 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     9121 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     9181 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     9241 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     9301 tagtggtcag agtcgctgtt tttcagcgat atggtgctgc caagcggtga taaagccctc
     9361 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
     9421 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     9481 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     9541 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     9601 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
     9661 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
     9721 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
     9781 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
     9841 gtgcggctgc acattgctgg gactactagg aagataaatg ccatcgagtt tcggcaaaag
     9901 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
     9961 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    10021 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    10081 cacaaaatgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    10141 ttttcaatct agcagtggat tttcattttt gcaaactcaa tttaacattt gacaaacatt
    10201 tggtttgcat acagattcga atgatggtca ttatatttta cgctttgata acgagcggaa
    10261 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    10321 gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg
    10381 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    10441 aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc
    10501 ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg gtcaggaaat
    10561 gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt ctgccgccga
    10621 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    10681 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt
    10741 cccggttgta gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg
    10801 ggatctgcaa ccgccctgcg cgcctggcac cgatgaccgc aatacacaaa gccaggttta
    10861 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg aactggcgca
    10921 gttgcagctg attccggcag atggcgcggt cgctgaggcc tcgccgggtc agtttgaaat
    10981 caacctttac catactgata acgtgctgga agcctgcgat gatgcgctgg cactaaaacg
    11041 cctagtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    11101 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    11161 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    11221 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    11281 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    11341 cgtcgccctg cgtattccgt gcggtgaccg tcataatcac cgcgtggaat atcacgtggc
    11401 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    11461 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    11521 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    11581 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    11641 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    11701 attccccttg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    11761 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    11821 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    11881 gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc
    11941 taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca
    12001 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    12061 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    12121 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    12181 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    12241 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    12301 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    12361 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    12421 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    12481 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    12541 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    12601 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    12661 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    12721 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    12781 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    12841 attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    12901 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12961 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    13021 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    13081 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    13141 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    13201 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    13261 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    13321 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    13381 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    13441 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    13501 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    13561 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    13621 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    13681 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    13741 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    13801 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    13861 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    13921 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13981 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    14041 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    14101 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    14161 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    14221 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    14281 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    14341 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    14401 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    14461 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    14521 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    14581 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    14641 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    14701 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    14761 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    14821 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    14881 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14941 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    15001 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    15061 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    15121 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    15181 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    15241 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    15301 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    15361 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    15421 cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    15481 caatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg
    15541 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattaccta ttctgccgct
    15601 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    15661 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    15721 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    15781 ttcgttggct tcagcttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    15841 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    15901 attaacggtc aaccaattgc cgaacagtta aaagagctct tcccggcgac gatagaactg
    15961 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    16021 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    16081 atcccggtgt tctggctggc gcttctgttg acactgtttt gttcactcac gctaggttgg
    16141 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    16201 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    16261 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    16321 ctgatgcgta tcagcactat tgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    16381 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    16441 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    16501 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    16561 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    16621 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    16681 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    16741 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcgggactgg ctgtactgtg
    16801 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    16861 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16921 cggacgtgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16981 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
    17041 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    17101 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    17161 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    17221 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    17281 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    17341 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    17401 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    17461 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    17521 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    17581 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    17641 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    17701 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    17761 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    17821 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    17881 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17941 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    18001 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    18061 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgaca
    18121 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    18181 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    18241 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    18301 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    18361 agcgcgatgc cgcataaaag tcgcctcaat actctacccg gcgctatccc attgctggaa
    18421 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    18481 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    18541 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    18601 ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
    18661 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    18721 actggcaaaa atgctggcgg gaatgataga acctaccagc ggcgagttat tgattgacga
    18781 tcatccgctg cattttggcg attattcctt ccgtagtcag cgtattcgca tgatttttca
    18841 ggatccttcg acctcgttga atccccgtca acgtatctca caaattctgg attttccact
    18901 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18961 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    19021 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    19081 tgaagccctc gcttcactgg atatgtcaat gcgttcgcag ttgattaatc tgatgctgga
    19141 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    19201 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    19261 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    19321 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    19381 ccagattatt cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    19441 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    19501 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    19561 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    19621 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    19681 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    19741 agattattct gcaggaactg aaccgccggt caccctcacc ctgaaagggc gagggggccg
    19801 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    19861 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19921 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttaggg
    19981 taaggggaaa agcgcccccc ctgccgcagc ctgctccggt cggacctggt aactatagct
    20041 actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac
    20101 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    20161 ctttccggta agcgcattct ggtaaccggt gttgccagca aactgtccat cgcctacggt
    20221 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    20281 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    20341 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    20401 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    20461 tatgttaacg ccgttacccg tgaaagcttc aaaattgccc acgacatcag ctcctacagc
    20521 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    20581 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    20641 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    20701 gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat caaagacttc
    20761 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtacagt tactattgaa
    20821 gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    20881 gtagtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga actgaaataa
    20941 tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
    21001 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
    21061 tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt
    21121 atcatgaaac gcaatccagt actacggagt atgacgttct gcctctggtc ccggaagccg
    21181 caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagagacg ctggccccct
    21241 tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gaccagctta
    21301 ccgtgacccg ttcacaaacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg
    21361 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
    21421 ccggacctga ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc
    21481 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
    21541 tcggtcttac tgtctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc
    21601 aggcgcggac aattgcgaca tttcaggctt atctcgggca tccggtacgc tggttacccg
    21661 ggctggcgat acaaaactac gccgacgcgt cactgtttgc tgatgaatca atacgctggc
    21721 gaagcagcta tgaggtggaa aaactacctg aagagcacac aaaaagttca actgcagaac
    21781 tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt ctcgaaaagt
    21841 tgttaaacag cacacgggtc aatgcacagc cggataatca gcttgcggcg ttgttatgcg
    21901 cgcgtataaa tggcagtcct gcttgttttt ccacctgtat ggattcatca aatgaatata
    21961 aaaaaatcag cacccttctg cgcaagggcg aaaatgaaat taaccgatgg gctgaccgtc
    22021 attctgttga gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
    22081 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
    22141 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
    22201 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
    22261 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
    22321 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
    22381 ttcctggaag tcgacgcaca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
    22441 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
    22501 ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat
    22561 gaccgtctga ccatcgttcc tgatcatcca cttttaaaag acgccattcc ttgccgcgca
    22621 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
    22681 catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt
    22741 gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga aaaagaagca
    22801 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgacctcg
    22861 ctggattttg tcaccatcga cagtgccagc actgaagata tggatgacgc ccttttcgct
    22921 aaggcgctgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
    22981 attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg
    23041 cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc
    23101 gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt
    23161 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac
    23221 caggtttctg actggctgga aaataccggt gactggaagc ctgaaagtga agcaattgcc
    23281 gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    23341 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    23401 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    23461 gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac
    23521 gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    23581 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    23641 gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt
    23701 gctgaaatta gctctgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc
    23761 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    23821 atcaaaggcg aaactgcgac gcgtccgcag gatgaaatca ctgtccaaat ggccgaacgt
    23881 cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg
    23941 aaagataaag ccgggaccgg cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc
    24001 ggcatgcgtg tgcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta
    24061 cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt
    24121 gaaacggttt acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    24181 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatgg
    24241 atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact
    24301 ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaaacaa
    24361 tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac
    24421 tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac
    24481 aactttctcg gatcgcacaa tccatactgg cggttgacgg aaagcagcga tgctttgcgc
    24541 ttttctacca ccaaaaccac agaacctgat cgtacattgc agttatctgc cgaacaggct
    24601 gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat
    24661 gaaagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc
    24721 aacgcgtctg catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc
    24781 tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga cagccggggg
    24841 aatatccaac gcttcaatcg gttatgtgaa gattacacag ggttgaaaga acacgacgtc
    24901 attgggtaaa gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc
    24961 aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca
    25021 cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaaa
    25081 aacgagattt ttttaatctg ttccggtacc gacattaccg aagagcgccg cactcaggag
    25141 cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg
    25201 caggagttaa tcgatcacgc tattaatcag gcagataaca ataaagttgg ggttgtgtat
    25261 cttgatttgg ataattttaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag
    25321 ttattacgcg acgtgtcatt ggctatttta agctgtctcg aacatgacca ggtgttggcg
    25381 cgtccaggtg gcgatgagtt tctggtactg gcatccaata cctcacaaag cgcgctggaa
    25441 gcaatggcat cacgaatttt gacccgctta cgcctcccct ttcgtattgg tttaattgaa
    25501 gtttatacca gctgttcagt aggtatttcg ctctctcccg aacatggttc ggacagcgcg
    25561 actattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa
    25621 ttttgcgttt ttactccaga aatgaatcag cgggtatttg aatatctctg gctggatacc
    25681 aacttgcgta aagcactgga aaacgatcag ttggttatcg aaaatcacct agcgtgtcga
    25741 agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc
    25801 gttggacttc atttcctacg ccgaagagtc agggctaatt gtgcctttag gccgttgggt
    25861 gattctcgat gtcgtacgcc aggtggcaaa gtggcgggat aaaggcataa acctgcgagt
    25921 ggcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
    25981 ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga cagagagttg
    26041 tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc aactaggtgc
    26101 gcaagtgcat ctggatgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
    26161 tccgatcgat gccatcaaac ttgaccaggt ttttgttcga gatattcaca aacaacctgt
    26221 ctcgcagtca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
    26281 cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
    26341 gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gctggtataa
    26401 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat atcccgtcat
    26461 tatatatcgt attcctggat gataattagc cgttgctgtt aaacacttct cttttagcgg
    26521 caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga tgtctgaatt
    26581 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
    26641 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
    26701 tgaatctacg ccagacatcc cgaagacacc gtgggagatt taacaatcgt gccttcaggt
    26761 tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta taacgttata
    26821 agaattacag cgatgaattc ccgacaacaa actattctac agatggtcat tgaccagggt
    26881 caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac cattcgccag
    26941 gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt tgcagtttcg
    27001 cttgatagtg atgacgtcga aacccgtatg atgagcaact atacgctgaa gcgtgaactc
    27061 gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca tctttatcga aaatggcagc
    27121 agcaatgccc tgctcgcccg gactctgggg gagcagaaga aaaatgtcac tatcatcacg
    27181 gtcagcagct acatcgcgca tttgctgaaa gacgcgcctt gtgaagtcat tttgctcggt
    27241 ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt tgacacgcca gtgcatccag
    27301 caggtacatt tcagcaaagc atttattggt attgatggct ggcaacctga aactggattc
    27361 accggtcgcg atatgatgcg caccgatgtg gtcaatgccg tgctggaaaa agagtgcgaa
    27421 gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc catcggtccc
    27481 gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga tctgatgcat
    27541 cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc cttctctacg
    27601 gatgatttgc agtttggcaa atcatccgct ctaagatgat tcctggttga taattaagac
    27661 tatttacctg ttattaacac tctcaagata taaaattatt atcagcgata taacaggaag
    27721 taattatcac ctgcgtgata taaccctgcg cgcgagcaga tttcacggaa taatttcacc
    27781 aggcttattc ttagctatta tagttataga gagcttactt ccgtgaatca taaattcagg
    27841 agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg caattacttt
    27901 ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gaccgcaaca ccgcaatcgg
    27961 cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt tgggtacatt
    28021 aggtggtgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat cgtaaataaa
    28081 tgtcgattat cgactcgcaa ttataatacg cttgattctg ttcagtacga aataactgaa
    28141 taatacacac agtaaaatat tcgaaagcca cggatatatc cgtggctttt tatgttaacc
    28201 gcctgcgagt tttaccttca tccctttcgc ttcaagtagt gattttaata aatcacgctt
    28261 atcgccctgg atttcaataa ctccatcttt aactgctccg ccgcagccgc attttttctt
    28321 cagttccgct gcaagttttg tcagttcggc atcatcgaga tcgataccgg taatcaggca
    28381 aacgccctta cctttacgtc cactggtctg acgctgaata cgcaccgctc catcgccttt
    28441 agggcgtact ggggccgctt tgggctcatc aatacgcccg gtttccgttg agtagaccag
    28501 acggctgttg gaatcactca tcatgcactc cgctgtaaag aggcgttgat cgctttcagt
    28561 gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa tcaccatata atcaacacca
    28621 gccgccaacg cctgttctgg cgtcataatg cggcgctggt caccagcttc actcccctgc
    28681 ggacgaatgc ccggtgtaac cagtttgaac tcctgaccga atacctgttt aaagcgcaca
    28741 gcttcctgag cagaacacac cacaccatca aggtcacatt tttgcgtcag tgctgccaga
    28801 cgttctgcat aatctgcagg tgacagtgtc acgccaagat cggccaggtc gctggcttcc
    28861 atgctggtca acactgtcac agcaatcaaa agcggtgcat ctttgccaaa cggaaccagt
    28921 gcctcacgcg ctgcggtcat catacgcgcc ccaccggagg catgaacatt caccatccac
    28981 acgcctaagt cagctgcagc agcgacagcg tgcgctgcag tgttggggat atcgtggaat
    29041 ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt cgcgcacaaa ctgtggccca
    29101 aacaatgtaa acatctcttt accgaccttc agacgacaat cgcgtgggtc gatcttgtcg
    29161 acaaaggaca gcgcgtcatc acaattatga taatcaaggg caacaaccac aggagaattc
    29221 gtaacggcgc gggaagaaga tgaagcagtt aacgtcatga ccagaccttc ttgatgatgg
    29281 gcaccgggag tggcgcgaaa caggtaaacg gcgagcattc tacccgtgaa aataaaaaat
    29341 taacagttgc gatttttccc ctagcaggct ttttctgctc cgtcgttgcc ggagcacaat
    29401 gggacatcaa taactagtat gttgtaacta aagtcggatt tttttaaaaa ttacaggcca
    29461 tcaagaccgc gaatcggttt aatagttgac caggcacgac aagacggaca atgccagtag
    29521 agggtgtatg cggtaaaacc acatttctgg cagctataac gaggcttact acgcaccttc
    29581 tcgccaacca tgtcacgcag caccatcaga ctctctttgg cgcgcccttc ttccgcttca
    29641 tttaagtggt aatccattag cttatggaat acacgcatgg tcggatgacg ctgaagctgg
    29701 cgtgtaatat agacctgtgc agcctcacta ccgtcgcgcg cttcgatgat atcagcaagc
    29761 atcaattcag catcggcacc ggtgttctct tccaccgcgc gttgcaggaa ttctgcccat
    29821 tcggcagttt tacccaactg ctgatagcag gtttgcaaca tttccagcgt ttcgctgacc
    29881 agttctctgt cctgggatat gacgcgttgc aaactttcga cggctttggc gtattctcct
    29941 ttcgccataa acacgcgtcc catcatgatg gatacgcggg cgctgttttt atctgccgcc
    30001 gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat atgctgcagg
    30061 gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc cagcttcacc
    30121 aggcgttcgg caacatcaat tgctttctgc cactcactgg tagcctggta gatttgtagc
    30181 aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt gaacatgtct
    30241 tccgcgcggt cgtataaccc ggcggccatg taatcacgcc ccagttgttg aatcgccaac
    30301 agacgctgtt cataggtcag cgaggcgctt tccattaggg tctgatggat gcgaatagcg
    30361 cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc ttcaacggtg
    30421 cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg ttgattacta
    30481 agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc atcttgcttg
    30541 ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac aggcaaaagc
    30601 agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg cggcactaca
    30661 gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg ttcagcgcgc
    30721 gccagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc gatagcaaac
    30781 cccgcagcaa acaataccgc cagcaatgtg gaaatacggt actccccttg cgctaacaga
    30841 taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat cacgaagatc
    30901 gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat gtggttagag
    30961 cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt cgcgccgtct
    31021 gcgaaatggc atccacgcgc attgttgatg atttaaggcc taaattcgga aaatcaacca
    31081 gcgttaactt tcttgttctc gttgtgcaat ttcgcgattt tcttccgcag gtggtgttaa
    31141 tggcccacaa attcgttgcg caagccacgt tgccaccgcc accagcgccc acgaaatcaa
    31201 cgtagctacc accagatcgc gtggccaatg catcccgagc agcaggcggc ttcccatgac
    31261 tcccgttgcc cagaccaaca agatagcaat ggttaacgtt cgccgacgcg gccacagcaa
    31321 accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag gaaaggcaaa
    31381 ccccgtctct ttctgccagt gtgaacgcaa atattgtgga atatttttct cttcagccaa
    31441 ctgttctttt actagatttc cgcgttctgc tcgctttaaa gtgtagaact catcaaccgg
    31501 aatatgatgt gttttttcca accagataac aaaaggtcgt ggttcctgta ctttgtcttt
    31561 gatccaggat ttaacgcctt gtccaacaag gattgccgcc gccagaatgg caaataatac
    31621 aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaaca aaatcaaatg
    31681 tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg cttttagtag
    31741 ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta ctggcatgac
    31801 aagcaatagt gcagctccca ctgcggtacg tcgggcaatc gaacgcatgg cctctccttt
    31861 ttgataagtc ccacaatcat aactgaaaat gccagttcca agaaaaattg acagatttgt
    31921 gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt tatggcaaaa
    31981 taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac gtgtggcaga
    32041 agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag aactggcaac
    32101 cggacacgat catgttgcgc tagtctatgg cgatatttcc gggcataccc cggtacttgc
    32161 gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct gcgattgtgg
    32221 cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta ttttgctgta
    32281 tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt acgcactgca
    32341 ggatcaaggt tacgataccg tagaggctaa ccaccagtta ggcttcgccg ctgatgagcg
    32401 cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag tccgcttgtt
    32461 aaccaataac ccgaaaaaag tcgaaattct gaccgaagca gggattaata ttgttgaacg
    32521 cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata ccaaagccga
    32581 gaaaatgggc catttgctga acaaataacc ctcttgcatc gtgtaattca tttgcttgcc
    32641 ggaagcaaaa taaccggcaa gcaaatactt gttacttcaa catattacga atgacataat
    32701 gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc ggtgtcgata cgacaacggc
    32761 agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt caccggaacc gtcgcgccag
    32821 gttgtagatt ttgcagatcg ccaatatcaa tcttctcttc cccggttagc cctaacgttt
    32881 tacgcgtcac gccttgcgga aattccagcg gcaggatgcc catgccaatt aaattcgaac
    32941 ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat tccaagcaga cgaggtcctt
    33001 ttgccgccca gtcacggctt gagcctgatc catactcttt cccggcaatc accgccagcg
    33061 gcgtttgctc ctgcttatag cgcatcgcag catcataaat agagactacg tcgctgtcag
    33121 gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg atgcgaatat
    33181 tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg taggagttaa
    33241 agtcttttcg ctcaacaccc cgaccttgta gatatcgacc cgctgggctg tcgggcttaa
    33301 tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt gcgaggatcc
    33361 gcgcaccgtg aatatcttcc actggtgctg gtgttgcctg catttcatca aagaaaggcg
    33421 ataagcgaat ataggttgag tcctcctgcc aaccgtaggt atcggatcgt gtgacgttaa
    33481 tttccttcca ctctgctgtg ccttcaaaaa cttctgcgta ctctttgcgg aacatttctg
    33541 tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata tctttcagat
    33601 aaaccggctc gcctttgcga tcatggccga taggctcaga agccaggttg atattcatat
    33661 ttcccgccag cgcataggca accaccagcg gtggcgaggc cagccagtta gttttaacca
    33721 gcggatggat acggccttca aagttacggt tgccggacag caccgcaccg acggttaaat
    33781 cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta ccaatacagg
    33841 tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt gtcagtttcg
    33901 cttttgccag ataatcagaa acgactttcg aacccggtgc caacgacgct ttgacccacg
    33961 gttgccgctt gaggccaaga gttacggctt ttttcgccag caagcctgcg gccatcagca
    34021 cacttgggtt agaggtgttg gtgcacgagg ttatcgcagc aatgaccaca gcgccatcag
    34081 gtaactgata ctgatgtccg ttcataacat aatcgactgg caggcgatct ttatgcgtgg
    34141 cattcacttt cagttcgtta ctggcggcaa atgcttttgg tacatcgggc agtgcaacgc
    34201 gatcctgtgg gcgttttggc cctgccaggc tcgcttcaac gtcattcata tccagttcta
    34261 acatactggt aaaaatcggt tcatcgcccg ggttacgcca catgccctgc gctttggcat
    34321 atttttcgac caactcgacc tgatcttcgc tgcgcccgct taaacgcatg taatcgaggg
    34381 ttactgcatc gattgggaag aagccacagg tggcaccata ttctggcgac atattggcaa
    34441 tggtggcgcg atccgccaac ggtagtgaat ccagaccatc accataaaat tcgacgaatt
    34501 tccccaccac gccatgtttg cgcagcattt gggtaacagt gagaaccagg tctgtggcgg
    34561 taataccttc acgtaatttt ccggtaagtt taaagcccac tacgtccggg ataagcatgg
    34621 aaaccggctg gcctaacatg gcggcttctg cttcgatccc acccacgccc caccccagca
    34681 cgccaaggcc gttgatcata gtggtgtgcg agtcagtacc aacgagtgta tccggataag
    34741 caatccattc accgtcctgc aattcgctcc acactgcttt gccgagatat tcgaggttaa
    34801 cctgatggca aatgcctgtg cctggcggca cgacgctaaa ccgactgaac gcttgctttc
    34861 cccatttcag gaacacataa cgttcgtggt tgcgctccat ttccaggcgt acgttttctt
    34921 caaatgcctc atcatcacca aaacgatcga cggtcaccga gtggtcaatg accaggtcga
    34981 ccggtgagag cgggttaacc tttgcagtat cgccaccgag gcgtttaacc gcttcgcgca
    35041 ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc acccttgccg
    35101 ggcggtaggc aatttcacgg tcagcatggg catttttcag ccatcccgcc agtgcgtgga
    35161 tatcctcttc ggtaactgag ttaccatcct gccagcgcag caggttttcg agcaaaactt
    35221 tgagtgactt gggtagacgg gtgatatcgc ccagtgattt agcagcaagc ggcaggctgt
    35281 agtagtggta agttttatct ttggcctgca acgtgtcctt actggcttct cgtagggttg
    35341 acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc agggtgaata
    35401 ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga taaaagagga
    35461 gaaaccttgt ggcaaagcaa atgacaaccc cgccgcagcg gggtcaagga tcagtgagta
    35521 atgatccaga ggagttcggc ccagaagagc atcgacagag aaaacgcgcc catccaggac
    35581 caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta ttttgtgttg
    35641 agataactca agctgttatt gctgcggtgt attaaaagcc ctttaattca ttaaaaatgg
    35701 gtaataagaa tgatgataat tattgtttcg gctgatcatc ttcagcaaac agttctataa
    35761 aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa cattcccgtt
    35821 tcgtttctgg ttcattgtca ctatgctggc ataatggtga cgactccagt tgctcctgac
    35881 cgaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc agaaaagtgc
    35941 agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggatcac ggcggggttc
    36001 ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca gcaagacgtt
    36061 gaatgattgg tgtaattaat catacgatga gacaaatcct aaagaaactt tagtcaaaaa
    36121 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
    36181 tttgaaaact ggaatttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
    36241 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
    36301 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
    36361 cagttttata tctttaaaca tgacctcaat ttcttcattg gagcgcaatg cgacagccgc
    36421 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
    36481 gcgcaaaaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
    36541 agcatcaacg cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
    36601 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
    36661 aacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
    36721 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
    36781 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
    36841 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagcga aatcggcatt
    36901 gcctttagag acggcatcag caatttgtgt cggcgggccc tggtgcatat gcaaagaaac
    36961 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
    37021 atgcgtggtg gcgatataca gcgagccttt atccggccag gtgtgctcac cggcaaccga
    37081 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgtcctgc
    37141 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
    37201 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
    37261 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
    37321 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
    37381 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
    37441 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
    37501 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
    37561 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
    37621 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
    37681 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
    37741 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaacacaccg
    37801 gcagcaccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
    37861 ggcaccggat cttctttcgg tggtgccact tcaccgttac gtaaaatctt acgtgtgttt
    37921 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
    37981 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
    38041 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
    38101 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
    38161 cgcagcgcgt tggtttcagc atcttcgcct tccagcacgt tcagcacttc gttttccggc
    38221 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcatagcc agaacagcca
    38281 aggaataccc cggtactcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
    38341 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
    38401 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
    38461 tcgtgatttg ccacctggtc gaggctgttt tccatctgcg cggtaaagtc gtagttcatt
    38521 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgcccatttt ttccgcatag
    38581 aaacgacgat tttctactcg cacatagcca cgatcctgaa tggtcgaaat gatcgacgca
    38641 taggtagacg gacgaccgat accacgtttt tccagttctt taaccagcga tgcttcactg
    38701 aaacgggctg gcggcttggt aaagtgctgg gctggtgtta gttcaacgag cgtcagagca
    38761 tcgcctttat cgactgccgg taagatacga tcttcatcgc ctttacgcag tgcaggcatc
    38821 acttttgtcc agccatcaaa gcgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
    38881 gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggcaacgaac
    38941 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
    39001 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
    39061 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
    39121 ccgcgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
    39181 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaaacca
    39241 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
    39301 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
    39361 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcattctg atgagtcacc
    39421 tgcaacgcca acgcttcacc agatggcgta gtcgtgctgg catcgacttc ccagaactct
    39481 tccggtacga acgctttaat ttcacgctca cgctctacca ccaggcggac cgccaccgac
    39541 tgcacacgac cggcagacag accacgagcg atctttttcc acagcagcgg cgaaaccata
    39601 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
    39661 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
    39721 actcggctat agcgcgcatc atcaccacca atcacttccc gcaggtgcca tgcaatggct
    39781 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
    39841 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
    39901 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
    39961 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
    40021 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cacaacccag atacttgttg
    40081 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
    40141 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
    40201 taccgatatg gactcgctct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
    40261 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg
    40321 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
    40381 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
    40441 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
    40501 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
    40561 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
    40621 cagcgtaatg gcgtgctggt atttaccggc gactactttt tagatgaaca aggattgcca
    40681 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
    40741 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
    40801 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
    40861 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
    40921 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
    40981 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
    41041 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
    41101 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
    41161 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
    41221 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
    41281 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
    41341 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
    41401 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
    41461 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
    41521 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
    41581 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
    41641 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
    41701 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
    41761 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt agccaaaaac
    41821 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
    41881 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
    41941 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
    42001 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
    42061 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
    42121 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
    42181 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
    42241 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
    42301 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
    42361 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
    42421 acgtcatgca gatcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
    42481 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttgga cgtcagggac
    42541 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
    42601 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
    42661 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
    42721 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
    42781 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
    42841 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
    42901 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
    42961 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
    43021 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
    43081 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
    43141 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
    43201 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
    43261 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
    43321 tcttgaactg gcggatacgg taagtgagct gcgccccatc agacatgcgt ttgatgccgg
    43381 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
    43441 catactttga ttaaccgtta ttacgcccgc cagaacgacg actgccgctc acctgactgt
    43501 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
    43561 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
    43621 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
    43681 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
    43741 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
    43801 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
    43861 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
    43921 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
    43981 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
    44041 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
    44101 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
    44161 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
    44221 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
    44281 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
    44341 tcgccttcac aggcgtcgaa caggagaatc aatggcgccg tatagcgcca ttttaaggcc
    44401 gcgtagtata cagagttacg cacttataag aaaggcttca catcacctac gccttcacgc
    44461 accacgaccg gcgtatcatc ggtaagatca ataaccgttg ttggtttctg cccgagatag
    44521 ccgccgtgaa taatcaaatc tacctgtttt tccagacggt ctttaatttc ttcgggatcg
    44581 gattcggtaa attcgctgcc tggcagcatt agcgaagtgg aaagcatcgg ttcgcccagt
    44641 gcttcaagca acgcctgcgc aatcgggtta gaaggcacac gcataccgat ggttttgcgt
    44701 ttctcctgca acagacggcg tggcacttcc ttcgtcccct tcaggatgaa ggtatagttg
    44761 cccggcgtgt tgtttttcat caaacgaaac gcaacgttat ccacgaatga ataggtcgag
    44821 agttcagaaa gatcgcgaca catcagggta aagttgtgac cgtccggcag ctggcgaata
    44881 cgacaaatac gctccatggc gtttttatct tcaattttac agccgagcgc atagccggaa
    44941 tcagttggat agacaatcac cccgccttta cgcacgatct ccaccgcctg gttgatcaga
    45001 cgttgctgcg ggttatcagg atgaatataa aaaaactggc tcataattcc ctctctgtgg
    45061 tgttctgcgg ctgttcccat agctgccaga cgccttcaac gcctgcgggc agccagagtt
    45121 tacgacccag ttcgatccac gggcatggct gatgaaaatc agatccttgt gatgcccata
    45181 aatgatgctg acgcgcaagt gtggccagct gggtgcgttc attgggcgat tgctggcact
    45241 gcgcgacttc catcgcgtcg ccgtggtgtt cggcaaaatg cgcaaccaat cttttcagcc
    45301 atttagcact aagattgtac cgcccaggat gagctagcac cgccttaccg ccagaatgat
    45361 gaatgacatc aatagcttgt tctattgtac accactgtgg cggaacgtat ccggttttcc
    45421 cgcgcgccag atacttttta aagacatctg ccattgaact ggctttgccg cactccacaa
    45481 ggaaacgcgc aaaatgaccg cgcgtcactg cgccgccctg cgccagtcgt tgtgcgcctt
    45541 ccagcgcccc aggtatttgc gctttttcaa gtctttcggc aatttgctgc gcccgctgat
    45601 tgcgccgttc tgtctgctgc gcgaggaact cacacattag cggatgagta atatcaatat
    45661 tcagcccgac aatatgaatt tcatgatttt cccagaccgt ggaaatttcc acgccgggaa
    45721 taagattcag cgccagtccc gaacgtgaaa tttcctctct tgctggcgcg attgcagctg
    45781 tggtgtcatg gtcggtgatc gccagggtgc cgacgcgcat ctcgactgca cggtgcacca
    45841 atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca
    45901 ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
    45961 atcataacgg gtctggcaaa tattctgaaa tgagctgttg acaattaatc atcgaaccag
    46021 ttaactagta cgcaagttca cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg
    46081 aaaggttggt ggcgcacttc ctgaaacggg cagtgtattc accatgcgta aagcaatcag
    46141 atacccagcc cgcctaatga gcgggctttt ttttgaacaa aattagagaa taacaatgca
    46201 aacacaaaaa ccgactctcg aactgctaac ctgcgaaggc gcttatcgcg acaacccgac
    46261 tgcgcttttt caccagttgt gtgggaatcg tccggcaacg ctgctgctgg aatccgcaga
    46321 tatcgacagc aaagatgatt taaaaagcct gctgctgata gacagtgcgc tgcgcattac
    46381 agcattaggt gacaccgtca caattcaggc actttccggc aatggcgaag ccctgctggc
    46441 actactggat aacgccctgc ctgcgggtgt ggaaagtgaa caatcaccaa actgccgtgt
    46501 gctgcgcttc ccccctgtca gtccactgct ggatgaagac gcccgcttat gctccctttc
    46561 ggtttttgac gctttccgtt tattgcagaa tctgttgaat gtaccgaagg aagaacgaga
    46621 agcaatgttc ttcggcggcc tgttctctta tgaccttgtg gcgggatttg aagatttacc
    46681 gcaactgtca gcggaaaata actgccctga tttctgtttt tatctcgctg aaacgctgat
    46741 ggtgattgac catcagaaaa aaagcacccg tattcaggcc agcctgtttg ctccgaatga
    46801 agaagaaaaa caacgtctca ctgctcgcct gaacgaacta cgtcagcaac tgaccgaagc
    46861 cgcgccgccg ctgccagtgg tttccgtgcc gcatatgcgt tgtgaatgta atcagagcga
    46921 tgaagagttc ggtggcgtag tgcgtttgtt gcaaaaagcg attcgcgctg gagaaatttt
    46981 ccaggtggtg ccatctcgcc gtttctctct gccctgcccg tcaccgctgg cggcctatta
    47041 cgtgctgaaa aagagtaatc ccagcccgta catgtttttt atgcaggata atgatttcac
    47101 cctatttggc gcgtcgccgg aaagctcgct caagtatgat gccaccagcc gccagattga
    47161 gatctacccg attgccggaa cacgcccacg cggtcgtcgc gccgatggtt cactggacag
    47221 agatctcgac agccgtattg aactggaaat gcgtactgac cataaagagc tgtctgaaca
    47281 tctgatgctg gttgatctcg cccgtaatga tctggcacgc atttgcaccc ctggcagccg
    47341 ctacatcgcc gatctcacca aagttgaccg ttattcctat gtgatgcacc tcgtctctcg
    47401 cgtagtcggc gaactgcgtc acgatcttga cgccctgcac gcttatcgcg cctgtatgaa
    47461 tatggggacg ttaagcggtg cgccgaaagt acgcgctatg cagttaattg ccgaggcgga
    47521 aggtcgtcgc cgcggcagct acggcggcgc ggtaggttat ttcaccgcgc atggcgatct
    47581 cgacacctgc attgtgatcc gctcggcgct ggtggaaaac ggtatcgcca ccgtgcaagc
    47641 gggtgctggt gtagtccttg attctgttcc gcagtcggaa gccgacgaaa cccgtaacaa
    47701 agcccgcgct gtactgcgcg ctattgccac cgcgcatcat gcacaggaga ctttctgatg
    47761 gctgacattc tgctgctcga taatatcgac tcttttacgt acaacctggc agatcagttg
    47821 cgcagcaatg gtcataacgt ggtgatttac cgcaaccata ttccggcgca gaccttaatt
    47881 gaacgcctgg cgacgatgag caatccggta ctgatgcttt ctcctggccc cggtgtgccg
    47941 agcgaagccg gttgtatgcc ggaactcctc acccgcttgc gtggcaagct gcccattatt
    48001 ggcatttgcc tcggacatca ggcaattgtc gaagcttacg ggggctatgt cggtcaggcg
    48061 ggcgaaattc ttcacggtaa agcgtcgagc attgaacatg acggtcaggc gatgtttgcc
    48121 ggattaacaa acccgctgcc ggtggcgcgt tatcactcgc tggttggcag taacattccg
    48181 gccggtttaa ccatcaacgc ccattttaat ggcatggtga tggcagtacg tcacgatgcg
    48241 gatcgcgttt gtggattcca gttccatccg gaatccattc tcaccaccca gggcgctcgc
    48301 ctgctggaac aaacgctggc ctgggcgcag cagaaactag agccagccaa cacgctgcaa
    48361 ccgattctgg aaaaactgta tcaggcgcag acgcttagcc aacaagaaag ccaccagctg
    48421 ttttcagcgg tggtgcgtgg cgagctgaag ccggaacaac tggcggcggc gctggtgagc
    48481 atgaaaattc gcggtgagca cccgaacgag atcgccgggg cagcaaccgc gctactggaa
    48541 aacgcagcgc cgttcccgcg cccggattat ctgtttgctg atatcgtcgg tactggcggt
    48601 gacggcagca acagtatcaa tatttctacc gccagtgcgt ttgtcgccgc ggcctgtggg
    48661 ctgaaagtgg cgaaacacgg taaccgcagc gtctccagta aatccggctc gtcggatctg
    48721 ctggcggcat tcggtattaa tcttgatatg aacgccaata aatcgcgcca ggcgctggac
    48781 gagttaggcg tatgtttcct ctttgcgccg aaatatcaca ccggattccg ccacgcgatg
    48841 ccggttcgcc agcaactgaa aacccgcacc ctgttcaatg tgctggggcc attgattaac
    48901 ccggcgcatc cgccgctggc gttaattggt gtttatagtc cggaactggt gctgccgatt
    48961 gccgaaacct tgcgcgtgct ggggtatcaa cgcgcggcgg tggtgcacag cggcgggatg
    49021 gatgaagttt cattacacgc gccgacaatc gttgccgagc tgcatgacgg caaaattaag
    49081 agctatcaat tgaccgctga agattttggc ctgactccct accaccagga gcaactggca
    49141 ggcggaacac ctgaagaaaa ccgtgacatt ttaacacgct tgttacaagg taaaggcgac
    49201 gccgcccatg aagcagccgt cgctgcgaac gtcgccatgt taatgcgcct gcatggccat
    49261 gaagatctgc aagccaatgc gcaaaccgtt cttgaggtac tgcgcagtgg ttccgcttac
    49321 gacagagtta ccgcgctggc ggcacgaggg taaatgatgc aaaccgtttt agcgaaaatc
    49381 gtcgcagaca aggcgatttg ggtagaaacc cgcaaacagc agcaaccgct ggccagtttt
    49441 cagaatgagg ttcagccgag cacacgacat ttttatgatg cgctacaggg tgcacgcacg
    49501 gcgtttattc tggagtgtaa aaaagcgtcg ccgtcaaaag gcgtgatccg tgatgatttc
    49561 gatccggcac gcattgccgc catttataaa cattacgctt cggcaatttc ggtgctgact
    49621 gatgagaaat attttcaggg gagctttgat ttcctcctca tcgtcagcca aatcgccccg
    49681 cagccgattt tatgtaaaga cttcattatc gacccttacc agatctatct ggcgcgctat
    49741 taccaggccg atgcctgctt attaatgctt tcagtactgg atgacgacca atatcgccag
    49801 cttgcagccg tcgctcacag tctggagatg ggggtgctga ccgaagtcag taatgaagag
    49861 gaactggagc gcgccattgc attaggggca aaggtcgttg gcatcaacaa ccgcgatctg
    49921 cgtgatttgt cgattgatct caaccgtacc cgcgagcttg cgccgaaact ggggcacaac
    49981 gtaacggtaa tcagcgaatc cggcatcaat acttacgctc aggtgcgcga gttaagccac
    50041 ttcgctaacg gttttctgat tggttcggcg ttgatggccc atgacgattt gcacgccgcc
    50101 gtgcgccggg tgttgctggg tgagaataaa gtatgtggcc tgacgcgtgg gcaagatgct
    50161 aaagcagctt atgacgcggg cgcgatttac ggtgggttga tttttgttgc gacatcaccg
    50221 cgttgcgtca acgttgaaca ggcgcaggaa gtgatggctg cggcaccgtt gcagtatgtt
    50281 ggcgtgttcc gcaatcacga tattgccgat gtggtggaca aagctaaggt gttatcgctg
    50341 gcggcagtgc aactgcatgg caatgaagat cagctgtata tcgatacgct gcgtgaagct
    50401 ctgccagcac atgttgccat ctggaaagca ttaagcgtcg gtgaaaccct gcccgcccgc
    50461 gagtttcagc acgttgataa atatgtttta gacaacggcc agggtggaag cgggcaacgt
    50521 tttgactggt cactattaaa tggtcaatcg cttggcaacg ttctgctggc ggggggctta
    50581 ggcgcagata actgcgtgga agcggcacaa accggctgcg ccggacttga ttttaattct
    50641 gctgtagagt cgcaaccggg catcaaagac gcacgtcttt tggcctcggt tttccagacg
    50701 ctgcgcgcat attaaggaaa ggaacaatga caacattact taacccctat tttggtgagt
    50761 ttggcggcat gtacgtgcca caaatcctga tgcctgctct gcgccagctg gaagaagctt
    50821 ttgtcagcgc gcaaaaagat cctgaatttc aagcccagtt caacgacctg ctgaaaaact
    50881 atgccgggcg tccaaccgca ctgaccaaat gccagaacat tacagccggg acgaacacca
    50941 cgctgtatct caagcgtgaa gatttgctgc acggcggcgc acataaaacc aaccaggtgc
    51001 tggggcaggc gttgctggcg aagcggatgg gtaaaaccga aatcatcgcc gaaaccgggg
    51061 ccggtcagca tggcgtggcg tcggcccttg ccagcgccct gctcggcctg aaatgccgta
    51121 tttatatggg tgccaaagac gttgaacgcc agtcgcctaa cgtttttcgt atgcgcttaa
    51181 tgggtgcgga agtgatcccg gtacatagcg gttccgccac gctgaaagat gcctgtaatg
    51241 aggcgctacg cgactggtcc ggcagttatg aaaccgcgca ctatatgctg ggcaccgcgg
    51301 ctggcccgca tccttatccg accattgtcc gtgagtttca gcggatgatt ggcgaagaaa
    51361 ccaaagcgca gattctggaa agagaaggtc gcctgccgga tgccgttatc gcctgtgttg
    51421 gcggcggttc gaatgccatc ggcatgtttg ctgatttcat caatgaaacc aacgtcggcc
    51481 tgattggtgt ggagcctggt ggtcacggta tcgaaactgg cgagcacggc gcaccgctaa
    51541 aacatggtcg cgtgggcatt tatttcggta tgaaagcgcc gatgatgcaa accgaagacg
    51601 ggcagattga agaatcttac tccatctccg ccggactgga tttcccgtct gtcggtccac
    51661 aacacgcgta tcttaacagc actggacgcg ctgattacgt gtctattacc gatgatgaag
    51721 cccttgaagc cttcaaaacg ctgtgcctgc acgaagggat catcccggcg ctggaatcct
    51781 cccacgccct ggcccatgcg ctgaaaatga tgcgcgaaaa cccggataaa gagcagctac
    51841 tggtggttaa cctttccggt cgcggcgata aagacatctt caccgttcac gatattttga
    51901 aagcacgagg ggaaatctga tggaacgcta cgaatctctg tttgcccagt tgaaggagcg
    51961 caaagaaggc gcattcgttc ctttcgtcac gctcggtgat ccgggcattg agcagtcatt
    52021 gaaaattatc gatacgctaa ttgaagccgg tgctgacgcg ctggagttag gtatcccctt
    52081 ctccgaccca ctggcggatg gcccgaccat tcaaaacgcc actctgcgcg cctttgcggc
    52141 aggtgtgact ccggcacaat gttttgaaat gctggcactg attcgccaga aacacccgac
    52201 cattcccatt ggcctgttga tgtatgccaa tctggtgttt aacaaaggca ttgatgagtt
    52261 ttatgcccag tgcgagaaag tcggcgtcga ttcggtgctg gttgccgatg tgccagttga
    52321 agagtccgcg cccttccgcc aggccgcgtt gcgccataat gtcgcaccta tcttcatctg
    52381 cccgccaaat gccgatgacg acctgctgcg ccagatagcc tcttacggtc gtggttacac
    52441 atatttgctg tcacgagcgg gcgtgaccgg cgcagaaaac cgcgccgcgt tacccctcaa
    52501 tcatctggtt gcgaagctga aagagtacaa cgctgcacct ccattgcagg ggtttggtat
    52561 ttccgccccg gatcaggtaa aagcagcgat tgatgcagga gctgcgggcg cgatttctgg
    52621 ttcggccatt gttaaaatca tcgagcaaca tattaatgag ccagagaaaa tgctggcggc
    52681 actgaaagtt tttgtacaac cgatgaaagc ggcgacgcgc agttaatccc acagccgcca
    52741 gttccgctgg cggcatttta actttcttta atgaagccgg aaaaatccta aattcattta
    52801 atatttatct ttttaccgtt tcgcttaccc cggtcgaacg tcaa
//
LOCUS       DAZSNO010000012        49711 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZSNO010000012 DAZSNO010000000
VERSION     DAZSNO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49711
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.012"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1224
                     /locus_tag="ACHWN2_000655"
     CDS             <1..1224
                     /locus_tag="ACHWN2_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR8930315.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="glpX"
                     /locus_tag="ACHWN2_000656"
     CDS             1221..2186
                     /gene="glpX"
                     /locus_tag="ACHWN2_000656"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR8930316.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="ACHWN2_000657"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="ACHWN2_000657"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFR8930317.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="ACHWN2_000658"
     CDS             2714..3427
                     /locus_tag="ACHWN2_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR8930318.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="ACHWN2_000659"
     CDS             complement(3399..4076)
                     /locus_tag="ACHWN2_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8930319.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="ACHWN2_000660"
     CDS             complement(4070..4747)
                     /locus_tag="ACHWN2_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8930320.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="ACHWN2_000661"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="ACHWN2_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5443..6021)
                     /locus_tag="ACHWN2_000662"
     CDS             complement(5443..6021)
                     /locus_tag="ACHWN2_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR8930321.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="ACHWN2_000663"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="ACHWN2_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="ACHWN2_000664"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="ACHWN2_000664"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR8930322.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="ACHWN2_000665"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="ACHWN2_000665"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR8930323.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHWN2_000666"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHWN2_000666"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR8930324.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="ACHWN2_000667"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="ACHWN2_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR8930325.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="ACHWN2_000668"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="ACHWN2_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR8930326.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="ACHWN2_000669"
     CDS             12291..13031
                     /locus_tag="ACHWN2_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR8930327.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="ACHWN2_000670"
     CDS             13198..14148
                     /locus_tag="ACHWN2_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8930328.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHWN2_000671"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHWN2_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHWN2_000672"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHWN2_000672"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR8930329.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHWN2_000673"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHWN2_000673"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR8930330.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHWN2_000674"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHWN2_000674"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR8930331.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHWN2_000675"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHWN2_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR8930332.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHWN2_000676"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHWN2_000676"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR8930333.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="ACHWN2_000677"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="ACHWN2_000677"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR8930334.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHWN2_000678"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHWN2_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR8930335.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="ACHWN2_000679"
     CDS             23768..23827
                     /locus_tag="ACHWN2_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR8930336.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="ACHWN2_000680"
     CDS             complement(23928..24530)
                     /locus_tag="ACHWN2_000680"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR8930337.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="ACHWN2_000681"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHWN2_000681"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHWN2_000682"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHWN2_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR8930338.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHWN2_000683"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHWN2_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR8930339.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="ACHWN2_000684"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="ACHWN2_000684"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR8930340.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHWN2_000685"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHWN2_000685"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR8930341.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHWN2_000686"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHWN2_000686"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR8930342.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="ACHWN2_000687"
     CDS             complement(29676..29759)
                     /locus_tag="ACHWN2_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930343.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="ACHWN2_000688"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="ACHWN2_000688"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR8930344.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="ACHWN2_000689"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="ACHWN2_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR8930345.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="ACHWN2_000690"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="ACHWN2_000690"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR8930346.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..35559
                     /locus_tag="ACHWN2_000691"
     CDS             34816..35559
                     /locus_tag="ACHWN2_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8930347.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="ACHWN2_000692"
     CDS             complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="ACHWN2_000692"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR8930348.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37094..37404
                     /gene="yqfB"
                     /locus_tag="ACHWN2_000693"
                     /pseudo
     CDS             37094..37404
                     /gene="yqfB"
                     /locus_tag="ACHWN2_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            37568..38227
                     /locus_tag="ACHWN2_000694"
     CDS             37568..38227
                     /locus_tag="ACHWN2_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR8930349.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38304..39284)
                     /gene="ygfZ"
                     /locus_tag="ACHWN2_000695"
     CDS             complement(38304..39284)
                     /gene="ygfZ"
                     /locus_tag="ACHWN2_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR8930350.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39527..39793
                     /gene="sdhE"
                     /locus_tag="ACHWN2_000696"
     CDS             39527..39793
                     /gene="sdhE"
                     /locus_tag="ACHWN2_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR8930351.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39774..40181
                     /locus_tag="ACHWN2_000697"
     CDS             39774..40181
                     /locus_tag="ACHWN2_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR8930352.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40221..40742)
                     /gene="fldB"
                     /locus_tag="ACHWN2_000698"
     CDS             complement(40221..40742)
                     /gene="fldB"
                     /locus_tag="ACHWN2_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR8930353.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40854..41750
                     /gene="xerD"
                     /locus_tag="ACHWN2_000699"
     CDS             40854..41750
                     /gene="xerD"
                     /locus_tag="ACHWN2_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR8930354.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41775..42485
                     /gene="dsbC"
                     /locus_tag="ACHWN2_000700"
     CDS             41775..42485
                     /gene="dsbC"
                     /locus_tag="ACHWN2_000700"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR8930355.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42491..44224
                     /gene="recJ"
                     /locus_tag="ACHWN2_000701"
     CDS             42491..44224
                     /gene="recJ"
                     /locus_tag="ACHWN2_000701"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR8930356.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44315..45413
                     /gene="prfB"
                     /locus_tag="ACHWN2_000702"
     CDS             join(44315..44389,44391..45413)
                     /gene="prfB"
                     /locus_tag="ACHWN2_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR8930357.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45423..46940
                     /gene="lysS"
                     /locus_tag="ACHWN2_000703"
     CDS             45423..46940
                     /gene="lysS"
                     /locus_tag="ACHWN2_000703"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR8930358.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(46983..47531)
                     /gene="idi"
                     /locus_tag="ACHWN2_000704"
     CDS             complement(46983..47531)
                     /gene="idi"
                     /locus_tag="ACHWN2_000704"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR8930359.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47586..47657
                     /locus_tag="ACHWN2_000705"
     CDS             47586..47657
                     /locus_tag="ACHWN2_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930360.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47654..47779)
                     /gene="yqfG"
                     /locus_tag="ACHWN2_000706"
     CDS             complement(47654..47779)
                     /gene="yqfG"
                     /locus_tag="ACHWN2_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR8930361.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47781..49229)
                     /gene="uacT"
                     /locus_tag="ACHWN2_000707"
     CDS             complement(47781..49229)
                     /gene="uacT"
                     /locus_tag="ACHWN2_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR8930362.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12023 a        12416 c        13373 g        11899 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattaattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    35281 tacgcggcat cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc
    35341 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    35401 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    35461 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    35521 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    35581 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    35641 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    35701 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    35761 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    35821 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    35881 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    35941 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    36001 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    36061 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    36121 agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta
    36181 aatccgcgac gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca
    36241 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    36301 catgagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    36361 atgcgacgcg cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg
    36421 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    36481 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    36541 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat
    36601 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    36661 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    36721 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    36781 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    36841 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    36901 gtgatttcgc gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct
    36961 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    37021 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac
    37081 aggaaatagt accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt
    37141 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    37201 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    37261 gcaacctcaa ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg
    37321 accctgcctg aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat
    37381 atgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt
    37441 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    37501 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga
    37561 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    37621 caacagcgtc agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact
    37681 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg
    37741 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    37801 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    37861 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    37921 tatctggagc ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa
    37981 aattttatct ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga
    38041 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    38101 ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca
    38161 cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca
    38221 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcgata
    38281 aatgcctgat gcgatacaga gatttactct tcgagcgaat acggcagcgg ctcgatatgc
    38341 agtgtattcg catcgtcgcg tacgcggaag atgctgtccg gttccatatc gttattcatg
    38401 acgacctgta ccacgacctg accatcttcc agttttaccg cagccagcac cgtaccggta
    38461 cgacgccagt tctcgcccat tttcagctct aagtcttcac cagcttccgg cagtcggctg
    38521 gcactacctg ccagcaacca gagcgcgcgt ttgttggcac cacggaattt tgctcgcgcc
    38581 accatctctt gtccggtata acagcctttc ttaaagctga taccgcccag cgcctggaga
    38641 ttggtcgcct gtgggataaa ctgcccgctg ttggcggcat caatcaccgg gaaaccagct
    38701 tcaatgttta atgccagcca ctgttggcta ttgttcagtt ccgcttcacc gcgcagttta
    38761 tcggtcagca tattagcagt agcttcatcg gttacgatca ggaaacgttc tgccgggtgt
    38821 tcaaaccata gcaaagtggt cgcgccttct ttgactacct gtttttcttt cgaaggcagt
    38881 tcgctaaaga gatttgccag cgcggcgcgc gcctgaaaac cggcaacacc aagcagcaca
    38941 cgctcgtcgt ctggcgcgat ggtcacttta gagaataccg catatttttt cagttcagtc
    39001 agctgcggtt cacgcacgct gcgccgttca atccatgcaa agccatcgcc gtcgcggaac
    39061 agacgtaaat tgctccacat tttacctttg gcgtcgcaat gggcggcgag caggtgctga
    39121 tcttctgcca tctggctgac atctgctgtc acctgaccct gcatatattt ttcgctgtcc
    39181 gcgccagtaa tggtggcaag cgcccagtca tcaagcgtca tcagcgtcag cggtaaacgg
    39241 gcagaagccg taggctgacg tggaggaaaa ggtgtaaaag ccataataga gtcctgagtt
    39301 gcttaacggc gtattaatga ctaatggtaa aagagctatg aggcaatgca agtgctttca
    39361 ctacgcgatc tgtgccttct gcgaggcgac tgccaaaaaa atgaagccct tgaattgttg
    39421 tgaattaatt tcgcgatcgc gatggtgttt actgatattc ccgtaaaaga agtcgaaaaa
    39481 cacgttacaa tagccgggta ctggattttc gtgagacagg aagaagatgg acattaacaa
    39541 caaagcccga attcattggg catgccgccg tggtatgcgc gaactcgata tttcaatcat
    39601 gccgtttttc gaacatgagt acgacagctt aagcgatgac gaaaaacgca tctttattcg
    39661 tctgctggaa tgtgacgatc cggacctgtt taactggctg atgaatcacg gtaaaccggc
    39721 cgatgcagaa ctggaaataa tggtccgact catccagaca cggaaccggg aacgtggtcc
    39781 tgtggcaatc tgatttgcgc gtctcctggc gcgcacagtg gctttccttg ctgattcatg
    39841 ggctggttgc cgctgttatt ttactcatgc cctggccgct cagttacacc ccgttatgga
    39901 tggtgttact ttcgctggtg gtatttgatt gcgttcgcag ccagcggtgt attaatgctc
    39961 gccaggggga aattcgcttg ttgatggacg ggcgtttgcg ttggcaaggg caggagtgga
    40021 gcatcgtcaa agcaccgtgg atgattaaga gcggcatgat gctgcgttta cgttctgatg
    40081 gcggtaaacg gcaacattta tggctggcag ccgacagcat ggacgaagcc gaatggcggg
    40141 atttacggcg gattttgttg caacaagaga cgcaaagata aagatgacgg caaagcatat
    40201 gcgctaagcc gtcagtgaga tcaggcgtaa tgctctgcca tttcgttgag gatttgctcg
    40261 caccagctct gaatacgctc gtcgctaagg tcatactggt tagtttcatc cagcgccaga
    40321 cccacgaaca gttgcccgtc agcaatcacc ggtttcgggc tggtaaattc atatccttcc
    40381 gttggccagt agccgacgaa cttcacgcct ttggtcgaga gtttgtcatg cagcataccg
    40441 agcgcatcga ggaaccactc gccgtatccc agttgatcgc caagcccata cagcgcaata
    40501 attttacctt caaggttcag gtcgtcgagc tgatcccaga cggcttccca gtcttcctgg
    40561 atttcaccaa aatcccaggt cgggataccc agaatgagca catcgtactg ctccattaat
    40621 ttcggggagt cgtccttgag gttatgtaag gtcaccagtt ctgggccgat aatatcgcgg
    40681 attttttctg ccgccatttc ggtgtaacag gtgctggaac cgtaaaaaag acccatattc
    40741 ataacgtaag cttctcgatt ctgctgtggc ttttgcagct agtgtatcag aatcgcttta
    40801 gtcacggcac aataaggcat aatggatcaa atgagtgacc ataaggggcg caagtgaaac
    40861 aggagctggc acgcatcgag cagtttcttg atgctctgtg gctggaaaaa aatctggctg
    40921 aaaatacgtt gaacgcttac cgtcgcgatc tgtcaatgat ggtggagtgg ttgcatcacc
    40981 gcgggttgac gctggcgacg gcgcaaagtg acgatttgca ggcattactg gcagaacggc
    41041 tggagggcgg gtataaagcc accagctcag cgcgtttgct gagtgcagtg cgccgattgt
    41101 tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc cagtgcgcat ctcgcttcac
    41161 cgaaattgcc ccagcgtttg ccaaaagatt taagcgaagc gcaggtcgaa cgtttattac
    41221 aggcgccatt aattgatcag ccactggagc tacgcgataa agccatgctt gaagtgttgt
    41281 atgctaccgg gctgcgtgtc tctgaactgg tcgggctgac aatgagtgat atcagcctgc
    41341 gtcagggcgt agtgcgggtc attggtaaag gcaacaaaga gcgcctggtg ccgttagggg
    41401 aagaggcggt ttactggctg gaaacctatc tggaacatgg gcgtccgtgg ctgctgaatg
    41461 gtgtgtcaat tgacgtgttg tttcccagcc agcgtgcgca gcagatgacg cgacagacct
    41521 tctggcaccg cattaaacat tatgccgtgc tggcgggtat cgacagcgaa aagctgtcac
    41581 cgcatgtgtt gcgtcacgct tttgccactc atttattaaa tcatggtgcg gatttacgcg
    41641 tggtgcagat gctactgggc cacagcgatc tttccaccac gcaaatttat acgcatgtcg
    41701 ctaccgagcg tctgcggcaa cttcatcaac agcatcaccc gcgggcgtga tgtctgaaaa
    41761 gaacgggaag atttatgaag aaaggtttta tgttgtttac tttgttagcg gcgttttcag
    41821 gctttgctca ggctgatgac gcggcaattc aacaaacgtt agccaaaatg ggcatcaaaa
    41881 gcagcgatat tcagcccgcg cccgtagctg gcatgaagac agttctgact aacagcggtg
    41941 tgttgtacat caccgatgat ggtaaacata tcattcaggg gccaatgtat gacgttagtg
    42001 gcacggctcc ggtcaatgtc accaataaga tgctgttaaa gcagttgaat gcgcttgaaa
    42061 aagagatgat cgtttataaa gcgccgcagg aaaaacacgt catcaccgtg tttactgata
    42121 ttacctgtgg ttactgccac aaactgcatg agcaaatggc agactataac gcgctgggga
    42181 tcaccgtgcg ttatcttgct ttcccgcgcc aggggctgga cagcgatgca gagaaagaaa
    42241 tgaaagctat ctggtgtgcg aaagataaaa acaaagcgtt tgatgatgtg atggcaggta
    42301 aaagcgtcgc accagccagc tgcgacgtgg atattgccga ccattacgca cttggcgtcc
    42361 agcttggcgt tagcggtact ccggcagttg tgctgagcaa tggcacactt gttccgggtt
    42421 accagccgcc gaaagacatg aaagaattcc tcgacgaaca ccaaaaaatg accagcggta
    42481 aataattcgc gtgaaacaac agatacaact tcgtcgccgt gaagtcgatg aaacggcaga
    42541 cttgcccgct gaattgcctc ccttgctgcg ccgtttatac gccagccggg gagtacgcag
    42601 tgcgcaagaa ctggaacgca gtgttaaagg tatgttgccc tggcagcaac taagcggtgt
    42661 cgaaaaggcc gttgagatcc tttacaacgc ttttcgcgaa ggaacgcgga ttattgtggt
    42721 cggtgatttc gacgccgacg gcgcgaccag cacggctcta agcgtgctgg cgatgcgctc
    42781 gcttggttgc agcaatatcg actacctggt accaaaccgt ttcgaagacg gttacggctt
    42841 aagcccggaa gtggtcgatc aggcccatgc ccgtggcgcg cagttaattg tcacggtgga
    42901 taacggtatt tcctcccatg cgggagttga gcacgctcgc tcgttgggca tcccggttat
    42961 tgttaccgat caccatttgc cgggcgacac attacccgca gcggaagcga tcattaaccc
    43021 taacttgcgc gactgtaatt tcccgtcgaa atcactggca ggcgtgggtg tggcgtttta
    43081 tctgatgctg gcgctgcgca cctttttgcg cgatcagggc tggtttgatg agcgcggcat
    43141 cgcaattcct aacctggcag aactgctgga tctggtagca ctggggacag tagcggacgt
    43201 cgtgccgctg gacgctaata atcgcattct gacctggcag gggatgagtc gcatccgtgc
    43261 cggaaagtgc cgtccaggga ttaaagcgct gctggaagtg gcaaatcgcg atgcacaaaa
    43321 actagccgcc agcgatttag gttttgcgct gggaccgcgt ctcaatgctg ccgggcgact
    43381 ggatgatatg tccgttggtg tggcgctgtt gctgtgcgac aacatcggcg aagcgcgcgt
    43441 gctggcaaat gaactcgatg cgctaaacca gacgcgaaaa gagatcgaac aaggaatgca
    43501 ggttgaagcc ctgaccctgt gcgagaaact ggagcgcagc cgtgacacgc tacccggcgg
    43561 gctggcaatg tatcaccccg aatggcatca gggcgttgtc ggtattctgg cttcgcgtat
    43621 caaagagcgt tttcaccgtc cggttatcgc ctttgcgcca gcaggtgacg gtacgctgaa
    43681 aggttccggt cgctccattc aggggctgca tatgcgtgat gcgctggagc gattagacac
    43741 actctacccc ggcatgatgc tcaagtttgg cggtcatgcg atggcggcgg gtttgtcgct
    43801 ggaagaggat aaattcgaac tctttcaaca acggtttggc gaactggtta ctgagtggct
    43861 ggccccttcg ctattgcaag gcgaagtggt gtcagacggt ccgttaagcc cggccgaaat
    43921 gaccatggaa gtggcgcagc tgctgcgcga tgctggcccg tgggggcaga tgttcccgga
    43981 gccgctgttt gacggtcatt tccgtctgct gcaacagcgg ctggtgggcg aacgtcattt
    44041 gaaggtgatg gtcgaaccgg tcggcggcgg tccactgctg gatggtattg cttttaatgt
    44101 cgataccgct ctctggccgg ataacggcgt gcgcgaagtg caactggctt ataagctcga
    44161 tatcaacgag tttcgcggca accgcagcct gcaaattatc atcgacaata tctggccaat
    44221 ttagcgtcat cttctctata aaaaagagcg tggattgggt acaatcccgc tcttatcacc
    44281 gcattttgac tagctcaata aaagaaatca gaccatgttt gaaattaatc cggtaaataa
    44341 tcgcattcag gacctcacgg aacgctccga cgttcttagg gggtatcttt gactacgacg
    44401 ccaagaaaga gcgtctggaa gaagtaaacg ccgagctgga acagccggat gtctggaacg
    44461 aacccgaacg cgcacaggcg ctgggtaaag agcgttcctc cctcgaagcc gttgtcgaca
    44521 ccctcgacca aatgaaacag gggctggaag atgtttctgg tctgctggaa ctggctgtag
    44581 aagctgacga cgaagaaacc tttaacgaag ccgttgctga actcgacgcc ctggaagaaa
    44641 aactggcgca gcttgagttc cgccgtatgt tctctggcga atatgacagc gccgactgtt
    44701 acctcgatat tcaggcgggg tctggcggta cggaagcaca ggactgggcg agcatgcttg
    44761 agcgtatgta tctgcgctgg gcagaatcgc gtggtttcaa aactgaaatc atcgaagagt
    44821 cggaaggtga agtggcgggt attaaatccg tgacgatcaa aatctccggc gattacgctt
    44881 acggctggct gcgtacagaa actggcgttc accgcctggt gcgtaaaagc ccgtttgact
    44941 ccggcggtcg tcgccacacg tcgttcagct ctgcgtttgt ttatccggaa gttgatgatg
    45001 atattgatat cgaaatcaac ccggcggatc tgcgcattga cgtttatcgc gcgtccggcg
    45061 cgggcggtca gcacgttaac cgtaccgaat ctgcggtgcg tattacccac atcccgaccg
    45121 ggatcgtgac ccagtgccag aacgaccgtt cccagcacaa gaacaaagat caggccatga
    45181 agcagatgaa agcgaagctt tatgaactgg agatgcagaa gaaaaatgcc gagaaacagg
    45241 cgatggaaga taacaaatct gacatcggct ggggcagcca gattcgttct tatgtccttg
    45301 atgactcccg cattaaagat ctgcgcaccg gggtagaaac ccgcaacacg caggccgtgc
    45361 tggacggcag cctggatcaa tttatcgaag caagtttgaa agcagggtta tgaggaacca
    45421 acatgtctga acaacacgca cagggcgctg acgcggtagt cgatcttaac aatgaactga
    45481 aaacgcgtcg tgagaagctg gcgaacctgc gtgagcaggg aattgccttc ccgaacgatt
    45541 tccgtcgcga tcatacctct gaccaattgc acgcagaatt cgacggtaaa gagaacgaag
    45601 aactggaagc gctgaacatc gaagtcgccg ttgctggccg catgatgacc cgccgtatta
    45661 tgggtaaagc gtctttcgtt accctgcagg atgtgggtgg ccgcattcag ctgtacgttg
    45721 cccgtgacga tctgccggaa ggcgtttaca acgagcagtt caaaaaatgg gacctcggcg
    45781 acatcctcgg cgcgaaaggt aaactgttca aaaccaaaac cggcgaactg tctatccact
    45841 gtaccgagct gcgtctgctg accaaagcac tgcgtccgct gccggataaa ttccacggct
    45901 tgcaggatca ggaagcgcgc tatcgtcagc gttatctgga tctcatctct aacgatgaat
    45961 cccgtaacac ctttaaagta cgctcgcaga tcctctctgg tattcgccag ttcatggtga
    46021 accgcggctt tatggaagtt gaaacgccga tgatgcaggt gatcccaggc ggtgccgctg
    46081 cgcgtccgtt tatcacccac cataacgcgc tggatctcga catgtacctg cgtatcgcgc
    46141 cggaactgta cctcaagcgt ctggtggtcg gtggcttcga gcgtgtattc gaaatcaacc
    46201 gtaacttccg taacgaaggt atttccgtac gtcataaccc agagttcacc atgatggaac
    46261 tctacatggc ttacgcagat tacaaagatc tgatcgagct gaccgaatcg ctgttccgta
    46321 ctctggcaca ggatattctc ggtaagacgg aagtgaccta cggcgacgtg acgctggact
    46381 tcggtaaacc gttcgaaaaa ctgaccatgc gtgaagcgat caagaaatat cgcccggaaa
    46441 ccgacatggc ggatctggat aacttcgact ctgcgaaagc gattgctgaa tctatcggta
    46501 tccacgttga gaagagctgg ggtctgggcc gtatcgttac cgagatcttc gaagaagtgg
    46561 cagaagcaca tctgattcag ccgaccttca ttactgaata tccggcagaa gtttctccgc
    46621 tggcgcgtcg taacgacatt aacccggaaa tcacagaccg ctttgagttc ttcatcggtg
    46681 gtcgtgaaat cggtaacggc tttagcgagc tgaatgacgc ggaagatcag gcgcaacgct
    46741 tcctggatca ggttgccgcg aaagacgcag gtgacgacga agcgatgttc tacgatgaag
    46801 attacgttac cgcactggaa catggcttac cgccgacagc aggtctgggg attggcatcg
    46861 accgtatggt aatgctgttc accaacagcc ataccatccg cgacgttatt ctgttcccgg
    46921 cgatgcgtcc ggtgaaataa gcattacgtt atgctcacaa ccccggcaaa tgtcggggtt
    46981 ttttatttaa gctgggtaaa tgcagataat cgtattctgg cttcgcgatt tgtcgcctgc
    47041 atcaccatcc acggactgaa cgcccacggc gtggcatcaa taccgcgtaa tacatcagct
    47101 aaatcacacc attgataatc catcacttca tcatcgttga tctgtaacgc actggtggtg
    47161 cgtgcggcaa ataccggaca cacttcattt tccacaatgc ctctcggatc ggtggcgcgg
    47221 tagcgaaagt caggatagat agattcagga ggcgtaattt ccacgccaag ctcataacgg
    47281 caacggcgga tcaccgcgtc ttcgctgctt tctcccagtt gtgggtgccc acaaaccgag
    47341 ttagtccaca cgccaggcca cgcttttttg ctcagtgcgc ggcgggtaac taataattgc
    47401 cccttggcat taaacagcca actggagaac gcgagatgta agagggtgtc tgccgtgtgt
    47461 gcggcatact tttccagcgt acccgtggga actccctgtg cattcaataa aatgacgtgt
    47521 tccgtttgca taatttctca catgtaattc tgatcactca tcattataga taattgaaga
    47581 gtgaaatgat taaccaagag agcgtttatc gacaaccgcc cgttttgagc ggatgccgac
    47641 aggtaaaaac catttaaaaa agtagcatca tatatttgaa gcgactttca atggccattg
    47701 caacacagtc agaaatgaca ggaatagaga gagtaaaaag caacaacaga ctgaatatag
    47761 cgcgcattaa aaaattcata ttattctcca tgctcatttt tcttcagctt gcgcatcaga
    47821 agccacatac gtactgtcct gacttctaaa tctttatcag agacggattc cttcaccagg
    47881 tcagcatgat gctgatagcc attaaagaag acgttcagca caacggcgct aagggttgcg
    47941 agcataatgc cgctatgtag cagcggttgc agtacggccg gtaacttaga aaagaaatcg
    48001 tgagagagcg tcggagtcat gccaacgccg agactgatcg ccacaatata gaggttgtaa
    48061 cggttggtgg tgtagttaca gcgcgacaga attcgaatcc ctgtcgccag taccatgccg
    48121 aacatcacta gaccagcgcc gcccagcaca aattgcggaa tggaggctac cagcaccgcc
    48181 atttttggca ccatgccaaa caggattaat ataattcccg aagaaataca cacccagcgg
    48241 ctatgaacgc gcatcacgct aaccaggcca acgttttgag aaaatgacgt gtgggggaag
    48301 ctgttaaacg tgccgcctat cattgtccct acgccatcga cacgcagccc gcaaataata
    48361 tcgtgtgaag agagtttacg accgactatt tcacccagtg ccaggaacat ccccatcgac
    48421 tcgataaaca cgatgattaa cacggcagtc atggtcagaa tggaaacggg atcgaaaatc
    48481 ggcataccaa acgacatcgg ggtgacaatc gcaaaccacg aggcatcatg tagcccggat
    48541 aaattgactt cattcatcat ccacgaaagt aaaaagccaa atacaatccc cagtaatacg
    48601 gcgacgttgg acataaatcc tttcgcatag cgagtaatga gcaagataaa aattaagacg
    48661 gcaaaggaga tacctaaata aacgggatta ccatattgcg gattcccttt acctccagcg
    48721 gcccagtcaa tacccacctg aatgatgcta agcccaatag aagtaataac cacaccggta
    48781 accagtggcg ggaataaagg catcaagcga ccaataagtg gcgctaataa tgtggtgata
    48841 aaacccgcgg cgatagtggc accaaatatc cccagcaggc cgatatccgg gttcatccca
    48901 atggctatca ttggtgttac agcagcaaag gtcaccgaca taatcaccgg caggcggatc
    48961 cccataaagc ggccgatacc gatacattgc aataatgtga cgatcccgca gcaaaagaga
    49021 tccgagctaa tgagcatcgc aatagcatct tttgagaggc ccagtcggtc accaatcata
    49081 agaggaacag cgactgcacc tgcgtacatg acaaggacgt gttgcagacc gagtatgatc
    49141 agctttcctg gtgataatat gcggtcaacc tcatcagttg ggcggtcttg cccagaagat
    49201 gagggaagtt gggaatctat ggcgctcatc agtgtttctc ctttcgcccg ccagaataaa
    49261 aagcaaccgt ttacgttaat gaaactgtct aaacacatgc tcgtggagtg ccagtttttg
    49321 cgaggaaggg gagttagaga caggaattgc ggaggcaatc acataatcgt gcttaaacca
    49381 cgaaaatgac aagagaaact gacaatggaa ttatctgtaa ttttctcata ttgatatttc
    49441 attaatcatt ctggcatgtc gcactctcgc atttaatcgt ttttatctgg atagcgctct
    49501 tttgatcggc aatgttgaaa taatgaaagc ggcatagatt gaggggcaat aatttgaact
    49561 cagtcgcaaa attataatta aaacggtcat gctacctaat atatgcccgt ttattgcatc
    49621 gcgaaatttg agaagacata actcattaaa tgaaagggat gcaaatgaat acgtttatcg
    49681 ctgctgaagc tgcggaatgt ataggctgtc a
//
LOCUS       DAZSNO010000013        49530 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZSNO010000013 DAZSNO010000000
VERSION     DAZSNO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49530
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.013"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHWN2_000708"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHWN2_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR8930363.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="ACHWN2_000709"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="ACHWN2_000709"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR8930364.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="ACHWN2_000710"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="ACHWN2_000710"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR8930365.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="ACHWN2_000711"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="ACHWN2_000711"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR8930366.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="ACHWN2_000712"
     CDS             5718..6659
                     /locus_tag="ACHWN2_000712"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR8930367.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="ACHWN2_000713"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="ACHWN2_000713"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR8930368.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="ACHWN2_000714"
     CDS             7679..8929
                     /locus_tag="ACHWN2_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR8930369.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /locus_tag="ACHWN2_000715"
                     /pseudo
     CDS             complement(9000..9694)
                     /locus_tag="ACHWN2_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="ACHWN2_000716"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="ACHWN2_000716"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR8930370.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="ACHWN2_000717"
     CDS             10866..12116
                     /locus_tag="ACHWN2_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR8930371.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="ACHWN2_000718"
     CDS             complement(12172..13260)
                     /locus_tag="ACHWN2_000718"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR8930372.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHWN2_000719"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHWN2_000719"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR8930373.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="ACHWN2_000720"
     CDS             complement(14194..15243)
                     /locus_tag="ACHWN2_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR8930374.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /gene="yieE"
                     /locus_tag="ACHWN2_000721"
     CDS             15342..16223
                     /gene="yieE"
                     /locus_tag="ACHWN2_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR8930375.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="ACHWN2_000722"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="ACHWN2_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR8930376.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="ACHWN2_000723"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="ACHWN2_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACHWN2_000724"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACHWN2_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="ACHWN2_000725"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="ACHWN2_000725"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR8930377.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /gene="yeiB"
                     /locus_tag="ACHWN2_000726"
     CDS             21991..23067
                     /gene="yeiB"
                     /locus_tag="ACHWN2_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR8930378.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="ACHWN2_000727"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="ACHWN2_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR8930379.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="ACHWN2_000728"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="ACHWN2_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR8930380.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="ACHWN2_000729"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="ACHWN2_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR8930381.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="ACHWN2_000730"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="ACHWN2_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR8930382.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACHWN2_000731"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACHWN2_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACHWN2_000732"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACHWN2_000732"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFR8930383.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30809..31048)
                     /locus_tag="ACHWN2_000733"
     CDS             complement(30809..31048)
                     /locus_tag="ACHWN2_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR8930384.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="ACHWN2_000734"
     CDS             complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="ACHWN2_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR8930385.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="ACHWN2_000735"
     CDS             complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="ACHWN2_000735"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR8930386.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32934..33629)
                     /locus_tag="ACHWN2_000736"
     CDS             complement(32934..33629)
                     /locus_tag="ACHWN2_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR8930387.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33626..34024)
                     /locus_tag="ACHWN2_000737"
     CDS             complement(33626..34024)
                     /locus_tag="ACHWN2_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR8930388.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34263..35210
                     /gene="dusC"
                     /locus_tag="ACHWN2_000738"
     CDS             34263..35210
                     /gene="dusC"
                     /locus_tag="ACHWN2_000738"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR8930389.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="ACHWN2_000739"
     CDS             complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="ACHWN2_000739"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR8930390.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35888..37324
                     /gene="mdtQ"
                     /locus_tag="ACHWN2_000740"
                     /pseudo
     CDS             35888..37324
                     /gene="mdtQ"
                     /locus_tag="ACHWN2_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37416..38138
                     /locus_tag="ACHWN2_000741"
                     /pseudo
     CDS             37416..38138
                     /locus_tag="ACHWN2_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38255..38833)
                     /locus_tag="ACHWN2_000742"
     CDS             complement(38255..38833)
                     /locus_tag="ACHWN2_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR8930391.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39003..39590
                     /locus_tag="ACHWN2_000743"
     CDS             39003..39590
                     /locus_tag="ACHWN2_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR8930392.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39764..40696
                     /gene="pbpG"
                     /locus_tag="ACHWN2_000744"
     CDS             39764..40696
                     /gene="pbpG"
                     /locus_tag="ACHWN2_000744"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR8930393.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40734..42449)
                     /gene="dld"
                     /locus_tag="ACHWN2_000745"
     CDS             complement(40734..42449)
                     /gene="dld"
                     /locus_tag="ACHWN2_000745"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR8930394.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42645..44942
                     /gene="bglX"
                     /locus_tag="ACHWN2_000746"
     CDS             42645..44942
                     /gene="bglX"
                     /locus_tag="ACHWN2_000746"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR8930395.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45194..46111
                     /gene="osmF"
                     /locus_tag="ACHWN2_000747"
     CDS             45194..46111
                     /gene="osmF"
                     /locus_tag="ACHWN2_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR8930396.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46118..47275
                     /gene="yehY"
                     /locus_tag="ACHWN2_000748"
     CDS             46118..47275
                     /gene="yehY"
                     /locus_tag="ACHWN2_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR8930397.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47268..48194
                     /gene="yehX"
                     /locus_tag="ACHWN2_000749"
     CDS             47268..48194
                     /gene="yehX"
                     /locus_tag="ACHWN2_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR8930398.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48199..48930
                     /gene="yehW"
                     /locus_tag="ACHWN2_000750"
     CDS             48199..48930
                     /gene="yehW"
                     /locus_tag="ACHWN2_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR8930399.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48911..49018)
                     /locus_tag="ACHWN2_000751"
     CDS             complement(48911..49018)
                     /locus_tag="ACHWN2_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR8930400.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49078..49530)
                     /locus_tag="ACHWN2_000752"
                     /pseudo
     CDS             complement(49078..49530)
                     /locus_tag="ACHWN2_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11955 a        12205 c        13311 g        12059 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacgaatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttattcat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggggggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    30841 cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat
    30901 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    30961 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg
    31021 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    31081 taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    31141 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    31201 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    31261 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc
    31321 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    31381 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    31441 accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt
    31501 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    31561 aatgcggtag cgataatact gattaataac gccggtacga taatattttg ctgtgccgag
    31621 caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    31681 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    31741 taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca
    31801 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    31861 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    31921 taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg
    31981 gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc
    32041 gggtaatcat aacccttgag attcaacaga attaacgccc cctgcaacgg cggcagagtc
    32101 gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc
    32161 agcgcgacac cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc
    32221 gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc
    32281 agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg
    32341 tgtgcctcgc ggcccggcaa atgaatacgc agatccagac cgctgttcag ttcattcata
    32401 aactgacggc agtgaccaca aggcgtatag ttaacggtga tggctgcaag cgctttttca
    32461 ccactcaacc aagcgtggct gatcgcgctt tgttcggcat gaacggtttg ctgcattgtt
    32521 gcaccaataa attccatatt ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt
    32581 gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt
    32641 gcgaatgcca gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga gacttgctcc
    32701 ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag
    32761 ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat
    32821 catgtaatgg gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat
    32881 taattatgca acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa attttagccc
    32941 attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat
    33001 aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc
    33061 gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata
    33121 cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg
    33181 caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc
    33241 gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat
    33301 gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc
    33361 gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg
    33421 accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtaact gtcgtaagag
    33481 atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc
    33541 aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc
    33601 ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac
    33661 gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga
    33721 cagcacagga aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga
    33781 cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg
    33841 gattgaccca tttcgccgga agaatttgca aggccagcag gacaaacagg atcagcatcc
    33901 cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca
    33961 ggcaggcata aatcaggacg aaagcgcgta aatattgcca gataatgttc agtgtcttac
    34021 tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta
    34081 ccgatcacat taagaaatca gcgcttattt tttcgctacc cggtattacg caatgagcag
    34141 cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgc
    34201 agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattttttt gcgggtgatg
    34261 atatgcgtgt gttactggca ccgatggagg gagtgcttga ctctctggtg cgtgaattgc
    34321 tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac
    34381 tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac
    34441 catctggtac gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg ctggcagaga
    34501 acgccgcccg tgcggtggag ttaggtttct ggggcgtgga tctcaattgc ggctgcccgt
    34561 cgaaaacggt taacggtagc ggcggcgggg cgacgttact caaagatcct gaactcatct
    34621 accagggggc aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag
    34681 tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg
    34741 ctggcgctac ggagctggtg gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc
    34801 atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca
    34861 acggtgaaat ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc ggctgcgacg
    34921 cagtgatgat tggtcgcggg gcgctcaata ttcccaacct gagccgggtg gtaaaatata
    34981 acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg
    35041 aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt
    35101 tgcgtaaaga atacgatgaa gcaacggaat tatttcagca tgttcgggtg ttgaataact
    35161 cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaccatagt
    35221 gatgtttttg acaatgtgtt agctctttat caataggccg cacggttgtt tggtggcgtc
    35281 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
    35341 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agccgagggc
    35401 accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg cggcgattgg
    35461 tcgccccctg cgcgctcctt gcgccagtga ttatatgaag caatgcacat gacaggagcg
    35521 caacaggatg agagactgaa atattctcgt tattttcgga tctgatgaca ttaattaatt
    35581 ttaaaatatc atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa
    35641 tacagcccag agtataattt tcattggcgt tcctttattt gtgagtgctg aataagtata
    35701 ggaaaaaacg aagccctttg ccgttatcca taaacgatta agataaatcg gaagatatca
    35761 tttacttagt ctatattttt ctgggtatgt cgttttattg ctactgatgt attgccacgc
    35821 gaaactaaaa aatatatcgc aacacacggt aatctccgcg caccgtttct tacctgatta
    35881 ttcccccatg aatcgtgatt ccttttatcc agccatcgcc tgttttccgc tgttactgat
    35941 gctggccggg tgtgcgccta tgcatgaaac ccgccaggcg ttaagccagc aaacgcccgc
    36001 tgcacaagtt gacaccgcat tacccacggc gctgaaaaat ggttggccag acagccaatg
    36061 gtggctggag tatcacgata atcagctcac ttcattaatt aacaatgcac tacagaacgc
    36121 accggatatg caggtggctg aacagcgcat ttagctagct gaagcccagg cgaaggcagt
    36181 tgccactcag gatggtccgc agatcgactt ttcggcggat atggagcggc aaaaaatgtc
    36241 ggcagaaggg ctaatggggc cgtttgctct gaacgatccg gccgcaggta cgaccggtcc
    36301 gtggtacacc aacggaactt ttggcttaac ggcgggctgg catctcgata tctggggaaa
    36361 gaatcgggcg gaggttactg cccgcctggg tacggttaaa gcacgggcgg cggaacgcga
    36421 gcaaacccgc caattgctgg ctggcagcgt agcccgcctg tactgggagt ggcaaaccca
    36481 ggcggcgtta aacacggtct tgcagcaaat agaaaaagag cagaacacca ttatcgcgac
    36541 cgatcgccag ctatatcaga acgggattac ttcttcagtt gacggtgtgg aaaccgatat
    36601 taatgccagc aaaacccggc agcagctcaa cgatgtcgcg gggaaaatga aaattattga
    36661 ggcacggtta aatgcactca caaatcacca gactaagtca ttgaagctta aaccggtcgc
    36721 gttgccgaaa gtggcaagcc agcttcctga tgaactgggg tactccttac tggcccggcg
    36781 ggcagatttg caggcggcgc actggtacgt tgagtcatcg ttaagcacta ttgatgcggc
    36841 aaaagcggca ttttatcctg acatcaacct gatggccttc ctgcaacagg atgcgttgca
    36901 cttaagcgat ctgttccgtc attccgcgca gcaaatgggc gttacggcag gcctgacgct
    36961 acctattttc gatagtggtc gtcttaacgc caatctcgat atcgcaaaag ccgaaagcaa
    37021 cttgtctatc gccagctaca acaaagcggt ggttgaagca gtgaatgacg tggcgcgggc
    37081 agccagtcag gttcagacac tggcggagaa aaaccagcat caggcgcaaa ttgagcgcga
    37141 tgccttgcgt gtggtaggtc ttgcgcaggc ccgctttaac gcgggcatca ttgctggttc
    37201 ccgcgtcagc gaagccagaa tcccggcgct gcgtgagcgg gccaatggcc tgttattgca
    37261 agggcagtgg ctggatgcct ccattcgact caccggtgcg ctgggcgggg gttacaaacg
    37321 ctgatgatat gcgccttcta tacttaacgt ttattcagcg ttaagtggag aactcgatgg
    37381 cataggtcgc gataattacc gcttccgatt cggggatcgg caaagagtgc gcgttattac
    37441 tggcgcagca ggggtttgat attggcatta cctggcactc agatgaagaa ggggcaaaag
    37501 ataccgcgcg tgaggtggtt acccacggcg tacgtgcgga gatcgtgcaa ctggatctcg
    37561 gcaatctgcc agaaggtgcg caggcgctgg agacactcat ccagcgattt gggcgtattg
    37621 atgtgctggt gaataatgcg ggtgcaatga ccaaagcgcc gtttcttgat atggcttttg
    37681 atgagtggcg caagattttt accgttgatg ttgatggtgc atttttatgc tcgcaaattg
    37741 cggctcgtca gatggtgaaa taagggcagg gcggtcgcat catcaacatt acgtcggtac
    37801 atgaacatac gccgctgccg gatgccagcg cctacacagc cgctaaacat gcgctcggcg
    37861 ggttaaccaa aacgatggcg ctggagttgg tcaggcataa gattttggtg aacgcagtcg
    37921 cgcctggggc gatagccacg ccaatgaaca gcatggatga tagcgacgtg aagcccgacg
    37981 cggagccttc gattcctcta cggcgttttg gcgcaacgca tgagattgcc agcctggtgg
    38041 tgtggctttg ttcggagggc gcaaattaca cgacagggca gtcgttgata gtggatggcg
    38101 gctttatgtt ggcgaatccg cagttcaacc cagaatagcg cagtgaggtg cgtggatatt
    38161 tcaatgggtt aggccagata agacaccagc gtcgcatcag gcatggaaca cccggtgccg
    38221 gatgtggagt gaatgcttat ctgtggcaac tgctttacgc ctgattatcc ggctttttct
    38281 tcccgcgccg tttcagccac cagcgcacgc ccaccaccag aaccacgacc agaatcaacc
    38341 agacccagtg cttcaaatgc tggtcgagat tgtgcaacca cggcgcaatc acctgaccac
    38401 cagcgtaacc aatagtggta aaaatcaacg cccaggcaaa tgcgccgaga atattcagcg
    38461 gcagaaagat tttcggcggc agctggctgg caccaatcag cgtcgggcca atcacccgaa
    38521 agccatacat aaagcgcgta ccaatgacaa acagatacgg atggcgttgg ataagtttct
    38581 gtgcccgctc aattttatcc tgatgtttcg agaaacggcg taacagcttg ccgccaaacc
    38641 gccgcccgca cagatagagc acctggtcgc caatcatgcc accaagcgcc acagaaagta
    38701 ccaccagtgg gaactttaat agcccctgat gtgccgcaac gcctcccagc aaagtcacgg
    38761 tttcaccttc cgccaggcta ccgataacca gcgcggcata accatattgt gagatgagtg
    38821 tattgagatc cataggccag tttgtctccc tttggttgcc tgtaagcata caccctgtga
    38881 atcattcacg ctgaaaaagg acatacatca gacggtgcaa attacatgga tgaacaaaaa
    38941 ataatcacaa gctggcttat acttgaactg tcaggttcat gaactcacaa caagggggcg
    39001 gtatgagcca tgtctgggga ctgttttccc atcccgatcg tgaaatgcag gtgattaatc
    39061 gcgaaaacga aacgatttct caccattaca cccaccacgt tttgctgatg gcggcgattc
    39121 cggtgatttg cgccttcatt ggcactacac agattggctg gaattttggc gatggcacta
    39181 tcctgaagtt atcctggttt accggactgg cgctggctgt cttgttttac ggcgttatgc
    39241 tggctggggt tgcggtgatg gggcgggtca tctggtggat ggcgcgcaat tacccgcagc
    39301 gtccgtctct tgcgcactgc atggtcttcg cgggctacgt tgcaactccg ctgtttttaa
    39361 gcggtctggt ggcgctttat ccactggtct ggttgtgtgc gctggtcggc acggtggcgc
    39421 tgttttacac cggctacctg ctttaccttg gtattccgag cttcctgaat atcaacaaag
    39481 aggaaggcct tagcttctcc agttcgacgc tggcgattgg cgtgctggta ctggaagtgc
    39541 tgctcaccct aaccgttata ctatggggct acggttatcg tttgttctag aaagttcttt
    39601 gacgttgcat tgctggcgta aatgtcagca atgcagcatt tactcacgat tctcctttga
    39661 cgatcgcact ttttgctcgt tatgatggcg tcgctagcct cagtaaatcc ttactccggc
    39721 ggtgcgcaac ccgtgcgcgt gaaccactat ctgaatgctc atcatgccga aatttcgagt
    39781 ttctttattt agcttggccc tgatgctggc tgtgcctttt gcaccgcagg ccgttgcgaa
    39841 aacggcagcc gctaccaccg cttcacaacc ggaaattgcc tccggtagcg cgatgattgt
    39901 tgatctgaat accaacaaag tgatctattc gaaccacccg gatctggtgc gtccgattgc
    39961 gtctatcagc aaattaatga ccgcgatggt tgtgctggat gcacgactgc cgctggatga
    40021 aaaactaaaa gtggatatca gccagacgcc ggagatgaaa ggggtctatt cgcgcgtacg
    40081 actgaatagc gaaatcagcc gtaaagatat gctgttgctg gcgctgatgt cttcagaaaa
    40141 ccgcgcggcg gcaagccttg cgcaccatta tcccggtggt tacaaagcct ttattaaggc
    40201 aatgaatgcg aaagcgaaat cgctcggaat gaacaacacg cgctttgttg aacctaccgg
    40261 attgtcggtg cataacgttt caactgcccg tgacttaacc aaactgctca ttgccagcaa
    40321 acaatatccg ttgatcgggc agttaagtac tacccgggaa gatatggcaa ccttctctaa
    40381 cccgacgtat acgctgccgt tccgcaatac taatcatctg gtgtatcgcg ataactggaa
    40441 tattcagtta accaaaaccg gctttaccaa tgcggcgggc cattgtctgg tgatgcgtac
    40501 ggttatcaat aataaaccgg tggcgctggt agtgatggac gcgtttggca aatataccca
    40561 ttttgccgat gccagccgcc tgcgtacctg gattgaaacc ggtaaagtga tgcctgtgcc
    40621 ggcagcagcg ttgagctata aaaaacaaaa agccgcccaa atggcggcgg cggggcagac
    40681 ggcacagaac gattaagtga attcggatgg cgatactctg ccatccgtaa tttttactcc
    40741 acttcctgcc agtttttccg tttactggtt ttaccgatcc ccggattcat gctattggtc
    40801 ggatcattct cgcgatagaa cttctgcaac gtctccggtg ctttatacaa atgaccgacg
    40861 ttatgctcgg cagggtactg cgcgccgcgc tgctgtagca gttccagcat ctgttctttt
    40921 aacgcatgca catccacgcc ttttttcact atgtaatcct gatggaagac atagcacata
    40981 aaatggccgt aatagagctt gtgcaccagc tggctgtcga tctccggcgg taaatgctca
    41041 taccactcgg tgtcgttacg ccgcagagcg atatccagtg ccagaatgtc ttcgacttca
    41101 tcggaatgca ccgcctgata acgaattgct gcgcccgcag cggcgaaacg gtgtaaaaac
    41161 gctttgctac cttcctccgg cgtacagaca aagaaatcgc cctcggcctg tttgaaataa
    41221 tccaccagcc acgatttggc ttcaccgacg ccatcgcctg ccatttttaa cagcagatga
    41281 tgctcgtatt tatcgcgcca gtttttcatg cgcggcggta aatggctggg gaacaggtga
    41341 ccgaattttt gcattgcacg gtcagtaaaa tgcggacgga agaatttcac tttctccagc
    41401 atcgcatcgg tgcgtccctt gagattaaag aagaacggca tcttgtcggt gcctaactta
    41461 tcaatcatca ggaaggtgtc tttgccgtat ttttccgcaa tatcgtagat atcccggtgc
    41521 atatattccc cggcaaccgg cagattttcg aagttagcca gaatatgacg gcggatttcg
    41581 gtcagcactt ccggctggtt ggtgccgata taaaacacct gctgattttt ttccgcttcg
    41641 aaggtatcaa gacgtaccgc aaagacggcc agcttcccgg cgcaaccaga agattcaaat
    41701 aaccgatccg gatcggcgtt ataacgtgcg ggcgtgtcgg cttcaatatc acgaacgcgg
    41761 tggacatagt cataatcgtg ggcgtgacga ccatcgtgac gcacatcgtc atctttgatg
    41821 cgatcgtcat ccagcttgct aaggatctgc tccggcgttt cgcccagatc aatccccaga
    41881 tggttcacca gcgtcagttt gccatcttca tctatacgcg cgaataacga catttcggta
    41941 tacgccgggc cacgttgcac cagcgagccg ccggagttgt tacaaatacc gccgatgacc
    42001 gatgcgccta tacacgatga tccaatcacc gaatgcggtt cgcgtcccag cggtttgagg
    42061 gctttttcca gcgaatagag cgtggtgccc ggataggcca gcacctgttc gcccttgccg
    42121 agaacgtgca gcttgtcgag acgcagggtg ctgatgatca cgatatcgcg atcatagtcg
    42181 ttaccgtttg gcgtcgatcc ttcggtcagg cctgtattgg cagcctgcat cagaataatt
    42241 ttgtcggcgg tgacgcaggc tttcagcacc cgccacaatt ccagtagtga gccagggaaa
    42301 acgacagcca gcgcgtcgcc ctgaccagaa cggaagccct tgcgatagcg ggccgttttt
    42361 gcgggatcgg tgagcaggtg tgaatgaccg accagacggg caagttcatt caaaaaggct
    42421 ttattatcag ttgttgtcat ggaagacatt ttccactcct tgtggtggcg aaaaaatatc
    42481 acaaactaga atagcgcctt gtatgacaat tcagcgagtg aatattttga aaattcagac
    42541 aataaactga gcattctttt tctctctcca tcatgcttat ggcaaactac ggtttttgca
    42601 ccattttcgc tgccacgtcg ggcaacaaag gaagaaaaat ccatatgaaa tggctatgtt
    42661 cagtaggaat cgcggtgagt ctggtcctgc agccagcact ggcggatgat ttattcggta
    42721 accatccatt aacgcctgaa gcgcgggatg cgttcgtcac cgatctgctc aagaaaatga
    42781 cggttgatga gaaaattggt cagctgcgct taatcagcgt aggcccggat aatccgaaag
    42841 aggcgatccg cgagatgatc aaagacggtc aggttggggc gattttcaac accgtaaccc
    42901 gtcaggatat ccgcgccatg caggatcagg tgatggaatt aagccgcctg aaaattcctc
    42961 ttttctttgc ttacgacgtg ctgcacggtc agcgcacggt gttcccgatt agcctcggtc
    43021 tggcctcgtc ttttaacctc gatgcggtga aaacggtcgg gcgtgtctct gcttatgaag
    43081 cggcagatga tggcctgaat atgacctggg caccgatggt cgatgtctcg cgcgatccgc
    43141 gctggggacg tgcttccgaa ggttttggcg aagatacgta tctcacctca ataatgggta
    43201 aaaccatggt ggaagcgatg cagggtaaaa gcccggcaga tcgttactcg gtgatgacca
    43261 gcgtcaaaca ctttgccgca tacggcgcgg tagaaggcgg taaagagtac aacaccgtcg
    43321 atatgagtcc gcagcgcctg tttaatgatt atatgccgcc gtacaaagcg gggctggacg
    43381 caggcagcgg cgcggtgatg gtggcgctga actcgctgaa cggcacgcca gccacctccg
    43441 attcctggct gctgaaagat gttctgcgcg accagtgggg ctttaaaggc atcaccgttt
    43501 ccgatcacgg tgcaatcaaa gagctgatta aacatggcac ggcggcagac ccggaagatg
    43561 cggtgcgcgt ggcgctgaaa tccggaatca acatgagtat gagcgacgag tactactcga
    43621 agtatctgcc tgggttgatt aaatccggca aagtgaccat ggcagagctg gacgatgctg
    43681 cccgccatgt actgaacgtt aaatatgata tggggttgtt taacgaccca tacagccatt
    43741 tggggccgaa agagtctgac ccggtggata ccaatgccga aagccgcctg caccgtaaag
    43801 aagcgcgtga agtggcgcgc gaaagcctgg tgttgctgaa aaaccgtctc gaaacgttac
    43861 cgctgaaaaa atcggccacc attgcggtgg ttgggccact ggcggacagt aaacgtgacg
    43921 tgatgggcag ctggtccgca gccggtgttg ccgatcaatc cgtgaccgta ctgaccggga
    43981 ttaaaaattc cgtcggtgaa aacggtaaag tgctgtatgc caaaggggcg aacgttacca
    44041 gtgacaaagg cattatcgat ttcctgaatc agtatgaaga agcggtcaaa gtcgatccgc
    44101 gttcgccgca agagatgatt gatgaagcgg tgcagacggc gaaacaatct gatgtggtgg
    44161 tggctgtagt cggtgaagca caggggatgg cgcacgaagc ctccagccgg accgatatca
    44221 ctattccgca aggccaacgt gacttgattg cggcgctgaa agccaccggt aaaccgctgg
    44281 tgctggtgct gatgaacggg cgtccgctgg cgctggtgaa agaagatcag caggctgatg
    44341 cgattctgga aacctggttt gcggggactg aaggcggtaa tgcaattgcc gatgtattgt
    44401 ttggcgatta caacccgtcc ggcaagctgc caatttcctt cccgcgttct gtcgggcaga
    44461 tcccggtgta ctacagccat ctgaacaccg gtcgtccgta taatgccgac aagccgaaca
    44521 aatatacttc gcgttatttt gatgaagcta acggagcgct ttatccgttc ggctatggtc
    44581 tgagctatac cactttcacc gtctctgatg tgaaactttc tgcgccgacc atgaagcgtg
    44641 acggcaaagt gacggccagc gtgcaggtga cgaacaccgg taagcgcgag ggggccacgg
    44701 tagtgcagat gtacttgcag gatgtgacgg cttccatgag tcgccctgtg aaacagctga
    44761 aaggctttga gaaaatcacc ctgaaaccgg gcgaaactca gactgtcagc ttcccgatcg
    44821 atattgaggc gctgaagttc tggaatcaac agatgaaata tgacgccgag cctggcaagt
    44881 tcaatgtatt tatcggcact gattccgcac gcgttaagaa aggcgagttt gagttgctgt
    44941 aatgctttag taagggcgcg acgtttgcgc cctttgcctg atgttcatat cttaacttgc
    45001 tgaattttcg ccctttgtag gccggataag gcgctcgcgc cgcatccggc aggaaacgcc
    45061 ggaccgaata tgcctcttaa gtatttatct gattctgcaa ctgaatcctt ccgctcaggc
    45121 taactctgtc attatcaact atgcttttct cttaattcgc tgaaaacaac agtaaaagaa
    45181 tgaggaaagc atcatgccac tcttaaagct ctgggcaggt tcactggtta tgttggcagc
    45241 cgtgagcctg ccgctacagg cggcttcccc cgttaaagtc ggttcaaaaa tcgataccga
    45301 aggcgcgctg ctcggcaaca tcatcttgca ggtactcgaa agccacggcg taccgacggt
    45361 gaataaagtg caactcggaa cgactcctgt ggggcgcggg gcgattactt ccgatgaact
    45421 ggatatctat ccggaatata ccggcaatgg cgctttcttc tttaaagatg aaaacgatgc
    45481 agcgtggaaa aatgccgggc agggctacga gaaagtcaaa aagctcgatg cggagcacaa
    45541 caagttaatc tggctgacgc ccgcgcctgc aaataacacc tggactatcg ccgtgcgtca
    45601 ggatgtggca gagaaaaaca aactcacttc gcttgccgac ctgagtcgtt atctgcaaga
    45661 gggcggcacc ttcaaactgg cagcctcggc agagtttatc gaacgcgccg atgcgttacc
    45721 cacgtttgaa aaagcctatg gctttaagct cggtcaggat cagttgctgt cactggctgg
    45781 tggcgacacg gcggtgacga ttaaagccgc tgcccagcaa acctctggcg ttaatgccgc
    45841 aatggcttac ggcactgacg gtccggtcgc ggcgctgggg ctgcaaacct taagcgatcc
    45901 gcaaggcgtg caacctatct acgcgcctgc accagtggtg cgtgaatcgg tgttgaagga
    45961 gtatccgcaa atggcacagt ggctacagcc agtcttcgcc agcctcgatg caaaaacatt
    46021 gcagcaactg aatgccagca ttgcagtgga aggactggat gccaaaaaag tggctgccga
    46081 ctacctgaaa caaaaagggt gggcgaagta atttcccgtg acttatctac gtattaatcc
    46141 tgttctggcg ctgctgttgt tgctgacggc aatcgcagcg gcgctgccgt ttatcagtta
    46201 cgcgcctaat cgtttagtct cgggtgaagg gcttcatctc tggcaactgt ggccgcaaac
    46261 gatctggatg ctggtgggcg ttggttgcgc ctggctgaca gcctgttttg tccccgctaa
    46321 aaaaggcagc atttttgcac tcattctggc gcaattcgtc ttcgtattgt tggtgtgggg
    46381 agctggaaag gcggcgaccc aactggcgca aaatggcagt gcgctggcgc gtaccagcct
    46441 cggcagaggt ttctggctgg ctgcggcgct ggcattgctg gcctgtagcg atgccatccg
    46501 ccgaatctcc acgcatccgc tgtggcgctg gttgttgcat atgcagattg ccattattcc
    46561 gctgtggttg ctgtactccg gcacgcttaa cgatctctca ctaatgaaag aatacgccaa
    46621 ccgtcaggat gtgtttgacg acgcgctggc acaacatctg acgttgctgt ttggtgcggt
    46681 gctgcctgcg ttagtgattg gtgtgccgtt gggcatctgg tgctactttt ccactgcgcg
    46741 gcagggggca attttttctc tgctcaatgt cattcagacc gtgccttcgg tggcgctctt
    46801 tggcctgttg attgcgccgc ttgccgcgct ggttacggcc tttccgtggc tggggaagct
    46861 cggcatagca gggaccggaa tgacacccgc actgattgcg ctggtgctct atgccttgct
    46921 gccgctggtg cgaggcgtgg tagtcggttt gaaccagatc ccgcgcgatg tgctggagag
    46981 cgccagagcg atggggatga gcggggcgca gcgtttcctg catgttcagt taccgctggc
    47041 gttaccggta tttctgcgca gcctgcgggt ggtgatggtg caaactgtag gtatggcggt
    47101 gattgcggcg ttaatcggcg caggcggttt tggtgcgctg gttttccagg ggctgctaag
    47161 cagcgccatt gatttagtgt tgctgggggt gatcccggta attgttctgg cggtgcttac
    47221 cgacgcgctg ttcgatttgc ttatcgcact gctgaaggtg aaacgtaatg attgaattta
    47281 gccatgtcag caaactgttc ggcgcacaaa aagccgttaa cgatctcaat ctcaattttc
    47341 aggaagggag tttttcggtg ctgattggca catctggctc cggcaaatcc accaccctga
    47401 aaatgattaa ccgcctggtg gagcatgaca gcggagtgat ccgctttgcc ggagaagaaa
    47461 ttcgctcgct gccagtactg gagttgcgcc gccggatggg ctatgccatt caatctattg
    47521 gcctgttccc ccactggagc gtggcgcaaa acatcgctac cgtgccgcaa ttacaaaaat
    47581 ggtcgcgggc gcggattgac gatcgtatcg acgaattaat ggcgctactg gggctggagt
    47641 caaatttgcg tgagcgttat ccgcatcagc tttccggtgg tcagcagcaa cgtgtgggag
    47701 tggcgcgtgc actggctgcc gatccgcaag tcttactgat ggatgaacct tttggcgcac
    47761 tggacccggt aacgcgcggc gcgttgcaac aagagatgac gcgcattcac cgtttgctgg
    47821 ggcgcactat tgtgctggtc actcatgata ttgatgaggc gctacggctg gcagaacatc
    47881 tggtattgat ggatcacggt gaagtggtgc agcaggggaa tccgctgacg atgctgactc
    47941 gtccggcgaa tgattttgtc cgccagtttt ttggacgtag tgaactgggg gtgcacctgc
    48001 tttcgttacg tagcgtggcg gattacgtgc gtcgcgaaga acgggcagaa ggtgaggcac
    48061 tggcagaaga gatgacgcta cgcgatgcgc tctccctgtt tgtcgcgcgg ggatgcgagg
    48121 tgctgccggt ggtgaacacg cagggccagc cttgcggcac gctgcatttt caggatctgc
    48181 tggtggaggc gtaagcgtat gaagatgttg cgcgatccgc tgttctggct cattgctctt
    48241 tttgtggcgc tgattttctg gctgccttac agccagccgc tgtttgctgc cttgttccca
    48301 caactgccac gacccgttta tcagcaagaa agttttgcag ctctggcact ggctcatttc
    48361 tggctggtgg gaatttcgag tttgtttgcg gtgatcattg gcacaggtgc cggaattgct
    48421 gttactcgcc cgtggggcgc ggaatttcgc ccactggtgg aaactattgc cgccgttgga
    48481 cagacttttc cgcctgtcgc agtgctggcg attgccgttc cggtgatcgg ctttggtctg
    48541 caaccagcga ttatcgcctt gatcctttac ggtgtgctgc ccatcctgca ggtgacactt
    48601 gccgggctgg gagcgattga tcccagcgtg acagaagttg cgaaaggtat gggaatgagt
    48661 cgaggtcagc gactgcgtaa ggtcgagcta ccgctggcgg ctccggtgat tctggcgggc
    48721 gtgcgaactt cggtgattat caacattggt acggcgacga tcgcctcaac ggtaggggcc
    48781 agtacgctgg gtacgcccat catcatcggg cttagcggat ttaataccgc gtatgtgatc
    48841 cagggggcgt tactggtggc actggcggcg atcatcgcag accgcctgtt tgaaaggctg
    48901 gtgcaggcgc ttagccagca cgcaaaataa aggtataacc tgcgagcatg acgccaccaa
    48961 ttccgcctaa cgccataaac aggaacaggg cgatgacccc aattttagct atgcgcataa
    49021 tgcactcctt atgttaacga aaggattgta cagtaaagcg catttgttaa cgaatcatta
    49081 aataccgagt gggaaaatat catggccttg ttcttgccaa ctggtgagtt gctgctgttg
    49141 ggcggaggtt cgattttcac cgcaccacac cagcaatgta cggccttcga atagttcagg
    49201 gcgtagttga ttgagcgagt gggcgagcga ggacatcaat gcgccatcct tgttgactgg
    49261 caatccagcc ctccagccac agacgggtgg tatcctgaat attccagcca accaccagcg
    49321 catctttacc ctgtttttta cgtgccgaag ccagacaaat ggcgatgtag ttgatcagta
    49381 cgccgtcgag gatcgccagc agcgcctgga gagtcggttg ttggcactga agccgtcggc
    49441 gcagaggaat aaacagatgt gtggtgagtg tctgggcggg gtaatcctga ccgcgctctt
    49501 tgatccacgt tcgcaggcta tgtagattgc
//
LOCUS       DAZSNO010000014        48604 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZSNO010000014 DAZSNO010000000
VERSION     DAZSNO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48604
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.014"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            425..1642
                     /gene="alaA"
                     /locus_tag="ACHWN2_000753"
     CDS             425..1642
                     /gene="alaA"
                     /locus_tag="ACHWN2_000753"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR8930401.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1726..2325
                     /gene="yfbR"
                     /locus_tag="ACHWN2_000754"
     CDS             1726..2325
                     /gene="yfbR"
                     /locus_tag="ACHWN2_000754"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR8930402.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLMKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2384..4216)
                     /locus_tag="ACHWN2_000755"
     CDS             complement(2384..4216)
                     /locus_tag="ACHWN2_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR8930403.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILTVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4303..4953)
                     /gene="hxpA"
                     /locus_tag="ACHWN2_000756"
     CDS             complement(4303..4953)
                     /gene="hxpA"
                     /locus_tag="ACHWN2_000756"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFR8930404.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEADLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4964..5458)
                     /locus_tag="ACHWN2_000757"
     CDS             complement(4964..5458)
                     /locus_tag="ACHWN2_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR8930405.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5541..5996)
                     /gene="yfbV"
                     /locus_tag="ACHWN2_000758"
     CDS             complement(5541..5996)
                     /gene="yfbV"
                     /locus_tag="ACHWN2_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR8930406.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6334..7536
                     /gene="ackA"
                     /locus_tag="ACHWN2_000759"
     CDS             6334..7536
                     /gene="ackA"
                     /locus_tag="ACHWN2_000759"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR8930407.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7611..9755
                     /gene="pta"
                     /locus_tag="ACHWN2_000760"
     CDS             7611..9755
                     /gene="pta"
                     /locus_tag="ACHWN2_000760"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR8930408.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9945..11465
                     /gene="yfcC"
                     /locus_tag="ACHWN2_000761"
     CDS             9945..11465
                     /gene="yfcC"
                     /locus_tag="ACHWN2_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR8930409.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11498..12040)
                     /gene="yfcD"
                     /locus_tag="ACHWN2_000762"
     CDS             complement(11498..12040)
                     /gene="yfcD"
                     /locus_tag="ACHWN2_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR8930410.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12098..12649)
                     /gene="yfcE"
                     /locus_tag="ACHWN2_000763"
     CDS             complement(12098..12649)
                     /gene="yfcE"
                     /locus_tag="ACHWN2_000763"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR8930411.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12705..13349)
                     /gene="yfcF"
                     /locus_tag="ACHWN2_000764"
     CDS             complement(12705..13349)
                     /gene="yfcF"
                     /locus_tag="ACHWN2_000764"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR8930412.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13485..14132
                     /gene="yfcG"
                     /locus_tag="ACHWN2_000765"
     CDS             13485..14132
                     /gene="yfcG"
                     /locus_tag="ACHWN2_000765"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR8930413.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14189..14551
                     /gene="folX"
                     /locus_tag="ACHWN2_000766"
     CDS             14189..14551
                     /gene="folX"
                     /locus_tag="ACHWN2_000766"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR8930414.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14572..15465
                     /locus_tag="ACHWN2_000767"
     CDS             14572..15465
                     /locus_tag="ACHWN2_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR8930415.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15513..16403)
                     /gene="rpnB"
                     /locus_tag="ACHWN2_000768"
     CDS             complement(15513..16403)
                     /gene="rpnB"
                     /locus_tag="ACHWN2_000768"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR8930416.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16600..17373)
                     /gene="hisP"
                     /locus_tag="ACHWN2_000769"
     CDS             complement(16600..17373)
                     /gene="hisP"
                     /locus_tag="ACHWN2_000769"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR8930417.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17381..18097)
                     /locus_tag="ACHWN2_000770"
     CDS             complement(17381..18097)
                     /locus_tag="ACHWN2_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8930418.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18094..18780)
                     /gene="hisQ"
                     /locus_tag="ACHWN2_000771"
     CDS             complement(18094..18780)
                     /gene="hisQ"
                     /locus_tag="ACHWN2_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR8930419.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18870..19652)
                     /gene="hisJ"
                     /locus_tag="ACHWN2_000772"
     CDS             complement(18870..19652)
                     /gene="hisJ"
                     /locus_tag="ACHWN2_000772"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR8930420.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19873..20655)
                     /gene="argT"
                     /locus_tag="ACHWN2_000773"
     CDS             complement(19873..20655)
                     /gene="argT"
                     /locus_tag="ACHWN2_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR8930421.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20921..21490)
                     /gene="ubiX"
                     /locus_tag="ACHWN2_000774"
     CDS             complement(20921..21490)
                     /gene="ubiX"
                     /locus_tag="ACHWN2_000774"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR8930422.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21585..23102)
                     /gene="purF"
                     /locus_tag="ACHWN2_000775"
     CDS             complement(21585..23102)
                     /gene="purF"
                     /locus_tag="ACHWN2_000775"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR8930423.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23139..23627)
                     /gene="cvpA"
                     /locus_tag="ACHWN2_000776"
     CDS             complement(23139..23627)
                     /gene="cvpA"
                     /locus_tag="ACHWN2_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR8930424.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23886..24548)
                     /gene="dedD"
                     /locus_tag="ACHWN2_000777"
     CDS             complement(23886..24548)
                     /gene="dedD"
                     /locus_tag="ACHWN2_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR8930425.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24538..25806)
                     /gene="folC"
                     /locus_tag="ACHWN2_000778"
     CDS             complement(24538..25806)
                     /gene="folC"
                     /locus_tag="ACHWN2_000778"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR8930426.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25876..26790)
                     /gene="accD"
                     /locus_tag="ACHWN2_000779"
     CDS             complement(25876..26790)
                     /gene="accD"
                     /locus_tag="ACHWN2_000779"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR8930427.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26946..27605)
                     /locus_tag="ACHWN2_000780"
     CDS             complement(26946..27605)
                     /locus_tag="ACHWN2_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR8930428.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27688..28500)
                     /gene="truA"
                     /locus_tag="ACHWN2_000781"
     CDS             complement(27688..28500)
                     /gene="truA"
                     /locus_tag="ACHWN2_000781"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR8930429.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28500..29513)
                     /locus_tag="ACHWN2_000782"
     CDS             complement(28500..29513)
                     /locus_tag="ACHWN2_000782"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR8930430.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29579..30715)
                     /gene="pdxB"
                     /locus_tag="ACHWN2_000783"
     CDS             complement(29579..30715)
                     /gene="pdxB"
                     /locus_tag="ACHWN2_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR8930431.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30814..31809
                     /gene="flk"
                     /locus_tag="ACHWN2_000784"
     CDS             30814..31809
                     /gene="flk"
                     /locus_tag="ACHWN2_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR8930432.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31806..32984)
                     /locus_tag="ACHWN2_000785"
     CDS             complement(31806..32984)
                     /locus_tag="ACHWN2_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8930433.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33239..34480)
                     /gene="fabB"
                     /locus_tag="ACHWN2_000786"
     CDS             complement(33239..34480)
                     /gene="fabB"
                     /locus_tag="ACHWN2_000786"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR8930434.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            34639..36645
                     /gene="mnmC"
                     /locus_tag="ACHWN2_000787"
     CDS             34639..36645
                     /gene="mnmC"
                     /locus_tag="ACHWN2_000787"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR8930435.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36766..37044)
                     /locus_tag="ACHWN2_000788"
     CDS             complement(36766..37044)
                     /locus_tag="ACHWN2_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR8930436.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(37078..37626)
                     /gene="epmC"
                     /locus_tag="ACHWN2_000789"
     CDS             complement(37078..37626)
                     /gene="epmC"
                     /locus_tag="ACHWN2_000789"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR8930437.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37626..38435)
                     /locus_tag="ACHWN2_000790"
     CDS             complement(37626..38435)
                     /locus_tag="ACHWN2_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFR8930438.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38435..39259)
                     /gene="mepA"
                     /locus_tag="ACHWN2_000791"
     CDS             complement(38435..39259)
                     /gene="mepA"
                     /locus_tag="ACHWN2_000791"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR8930439.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39263..40348)
                     /gene="aroC"
                     /locus_tag="ACHWN2_000792"
     CDS             complement(39263..40348)
                     /gene="aroC"
                     /locus_tag="ACHWN2_000792"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR8930440.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40383..41315)
                     /gene="prmB"
                     /locus_tag="ACHWN2_000793"
     CDS             complement(40383..41315)
                     /gene="prmB"
                     /locus_tag="ACHWN2_000793"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR8930441.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            41481..42032
                     /gene="smrB"
                     /locus_tag="ACHWN2_000794"
     CDS             41481..42032
                     /gene="smrB"
                     /locus_tag="ACHWN2_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR8930442.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42136..43026)
                     /locus_tag="ACHWN2_000795"
     CDS             complement(42136..43026)
                     /locus_tag="ACHWN2_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR8930443.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(43013..43537)
                     /locus_tag="ACHWN2_000796"
     CDS             complement(43013..43537)
                     /locus_tag="ACHWN2_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8930444.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43534..44004)
                     /locus_tag="ACHWN2_000797"
     CDS             complement(43534..44004)
                     /locus_tag="ACHWN2_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8930445.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(44001..44549)
                     /locus_tag="ACHWN2_000798"
     CDS             complement(44001..44549)
                     /locus_tag="ACHWN2_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8930446.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44554..45276)
                     /locus_tag="ACHWN2_000799"
                     /pseudo
     CDS             complement(44554..45276)
                     /locus_tag="ACHWN2_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(45296..47936)
                     /gene="yfcU"
                     /locus_tag="ACHWN2_000800"
                     /pseudo
     CDS             complement(45296..47936)
                     /gene="yfcU"
                     /locus_tag="ACHWN2_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(48018..48581)
                     /locus_tag="ACHWN2_000801"
     CDS             complement(48018..48581)
                     /locus_tag="ACHWN2_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8930447.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        12062 a        13060 c        11976 g        11506 t
ORIGIN      
        1 atattaaata ataattaatt agcatcatcc tcattcatta attccgctta acaatagttt
       61 cacaccttac ccccattgcg acacctcgga aatcatcacg ctgttaaact ctccgtaatg
      121 cggaaatatc atcatcgcgt ttcattagtg aattcttaaa tgaggcattt tcacacaatt
      181 atcttacaga taaaaaaacc agacttgcaa ttaagaatta gaacaattca ctatataaca
      241 ttgcatgtaa agcatataag cctcattatt tgtcattatt aagttattaa cagcacaatc
      301 gagccttccc ctctggcaaa atcttattct gcagaccttc aaaacaccgt cctgggggag
      361 tacattgttc taagctgact tccacggcag ggagtggcga taacagcaaa aaaggtcaag
      421 attcatgtcc cccattgaaa aatccagcaa attagagaat gtctgttatg acatccgtgg
      481 tccggtgctg aaagaagcaa aacgcctgga agaagaaggt aacaaggtac tgaaactgaa
      541 catcggcaac ccagccccat tcggttttga cgcgccagat gaaatcctcg ttgacgtgat
      601 acgcaacctg cctaccgctc aagggtattg cgattccaaa gggctttact ccgcgcgtaa
      661 agccatcatg cagcactacc aggctcgtgg catgcgtgat gttaccgtgg aagatattta
      721 catcggcaat ggtgtatcgg agcttatcgt tcaggcaatg caggcattgc tgaacagcgg
      781 ggacgaaatg ttggttcctg caccagatta cccactctgg accgcggcgg tttcgctttc
      841 cagcggtaaa gcggtgcatt atctttgcga tgaatcctct gactggttcc cggacctcga
      901 tgatattcgc gctaaaatta cgcctcgtac gcgtgggatt gttattatca acccaaataa
      961 cccaaccggc gcggtatatt ccaaagagct tttaatggag attgtggaga ttgcacgtca
     1021 gcataatctc attatcttcg ccgatgaaat ttatgacaaa attctctacg acgatgctga
     1081 gcatcactca attgcgccgc tggcacctga cctgctgacc attaccttta acggactgtc
     1141 gaaaacgtac cgcgttgcag gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa
     1201 acacgccaaa ggctacatcg aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa
     1261 cgttcctgcg caacacgcca ttcagaccgc gctgggtggt tatcagagca tcagtgaatt
     1321 tattacccct ggcggtcgtc tttatgagca gcgtaaccgc gcgtgggaac tgatcaacga
     1381 tattccgggc gtttcctgcg tgaaacctcg tggtgcgctg tatatgttcc cgaaaatcga
     1441 cgccaaacgc tttaacattc atgacgatca gaaaatggtg ctggatttcc tgttgcagga
     1501 aaaagttctg ttggtgcaag ggacggcatt caactggccg tggccggatc acttccgcat
     1561 tgtcacgcta ccgcgtgtcg atgatatcga gctgtctttg agcaagttcg cgcgtttcct
     1621 ttctggttat catcagctgt aatcttaatt tcactgccgg agattgcatc cggcagtgtt
     1681 atcccgccac aatgacctga tgatgtcatc atacgtaagg tcactatgaa acagagccat
     1741 ttttttgccc atctctcccg cctgaaactc attaaccgct ggccgctaat gcgcaacgtg
     1801 cggacggaaa atgtgtccga acacagtttg caggtagcga tggtcgccca tgcgctggca
     1861 gctatcaaaa atcggaaatt tggcggtaat gtcaacgccg aacgtatcgc tttactggcg
     1921 atgtaccacg atgcctcaga agtgctcacc ggcgatctcc ctactccggt gaaatacttc
     1981 aatgcgcaaa tcgctcagga gtacaaggct attgaaaaaa tcgctcagca gaaactggtc
     2041 gatatggttc cggaagaact gcgggatatc tttgcgccgt taattgacga gcatgcatat
     2101 agcgatgaag aaaaatcgct gatgaaacag gcagatgcac tgtgtgcata tctgaaatgt
     2161 ctggaagaac tcgcggccgg aaataatgaa ttcttgctgg caaaaacgcg actggaagcg
     2221 acgcttgaag cgcgtcgcag ccaggagatg gactacttca tggaagtatt tgttcccagc
     2281 ttccatcttt cgctcgatga gattagccag gattcaccgc tgtaagcagc cggagtctgc
     2341 gtcgcatcag tcaataagcg ccggatgcga catcaggctc ttgtcaaaac ggaaacagca
     2401 tcgggatcat caccacacaa accgccatca cgataatggt gaacggtacc cccaacttca
     2461 caaagtcact gaagctgtaa tttcccggac ctaaaaccag ggtgttaaca ggtgaagaca
     2521 ccggtgtcat aaaggcggcg gatgctgcca tcgccacgac catcgcaaat ggataaggcg
     2581 acactcccat cgttttggca gcagccagcg caatcggagc catcaacacc gccgtcgcgg
     2641 tattagagat aaacagccca ataaccgccg acaagacaaa caaacacccc agcatcatat
     2701 gtggcccata accgccgcca atatccatca gccctttcac cgccagcgcg acacctcccg
     2761 ttttctgtaa tgccacagca aacggcatca tcccaacggt caaaataatg ctcggccagt
     2821 gaatggattt ataggcgctt tcagcatcta tacagcggaa tttccccatc agcaggcagg
     2881 cgatgatagc ggcaacagga ttaggaattt catttgtcag cattaacgcc accattagca
     2941 ccagacagaa aatggcatgg ggtgcctggc tgtgcgcggg cgatgcttca ctcacctctt
     3001 ccggtaagtt cagcgctacg aagtcgcggc cctgtttggc cagcataccg atcagtttcc
     3061 agttacccac aaccaggatg atatcgccca gcagcagagg ctcatccgcc agcgaacctt
     3121 ccagcgccac gccattgcgc ttgagcccca ccacattcag tccgtagcgg gtacgaaaac
     3181 caatttcgcg caccgattta ccaatcagtt ctgactcagg aattaatgaa atctctgcca
     3241 tgcccacatc aagggcctgg tcagaaaaat actcgccgcg cagtaccatc ggctccagca
     3301 attgctcact acaaaattgc cggagatcga catcagccgc agacatatca ataagcaaaa
     3361 cgtcacgcgc gcgaaattca gaaaccccat taacgttcac gataacgcga cgaaaacgcc
     3421 gccagcgttc aacaccgatg acgttagcgc cataacgctc acgtaatttg agatcatcca
     3481 gccgttgacc tatcattggc gatccggggc gaatagccag acgtcgcgca cgcccggtca
     3541 gtcgatattc acggataaga tcgcgaaagg ttcgacgcgt ccagccttcg cgctgcgggg
     3601 tctgggtatc ccctttcagc atgaaacgca tcactaacat atacaaaata cccagcacca
     3661 gcacgaccag gccaataggt gttacgctaa agaaactgaa gccgtgatag ccttcacgca
     3721 gcaattcact gttgactacc aggttcggcg gcgtcgccac cagcgtcatc atgccgctaa
     3781 tcagcccggc aaaacttaac ggcatcatca gacgtgacgg cgaagtttgc atacgcatgg
     3841 caacgcttaa caccacggga ataaagatag cgacaacgct ggttgaactc ataaacgcgc
     3901 caagccccgc gacggtcagc atcaacaaaa ccaacatttt gatttcactg ttgcccgcca
     3961 ctttgaccag ccatgttccc attacggtgg caacaccggt acggaccaaa ccatcgccaa
     4021 taataaacaa ggcggcaatc aggacaacgt taggatcaga aaagccggaa aatacttcgg
     4081 ggaccgtcag cgttccgctt aatgcaaacg cgacaataac aaacaaagcg accgcatcca
     4141 tacgcactct gcccgtcgca aacaagacga tggcaacggc cagtaatgaa agaacccaaa
     4201 tcaattcacc gttcacaact tatccttgtt aattgagggg gatgacttga ttctgccata
     4261 aaaaagcccc gacgagacgg ggctaaatca tgatcaggtg tttcactgaa taataacatc
     4321 gccatttggc tgtttggtca cagtaatttg ctccagactg tggaggacca aatcggcctc
     4381 attcaggcgc ggggtatctg ccggagcgtt aaccgcaatg acatggcaac ccgccgccag
     4441 gccagaaagc acgccagcgg gagcatcttc caccaccaca cactcctgcg gcgcaagccc
     4501 cagcagctgc gcacctaaca gatacgcatc aggttctggt tttccgcgct tcactcgctc
     4561 agcggttaca aacacctctg gtgcgggaag ccccgctatt ttatggcgcg ctcgcgctac
     4621 cggcatggag ccagaagtca caatggccca cggaatacct gctttattca aatgattgag
     4681 taaggcgatt gccccaggaa gcgcggtaat accttcggtt tccgtggcct cgatttgctc
     4741 cagacgcgta aactcggcgg caatatcagc ctcggatttg cccgccataa aatggcgcaa
     4801 agaggtgatc gcctgtttac cgtgaatgaa agccagcacc tcttccggcg ctaacccatg
     4861 acgtctggcc cagttgctcc acgcccgttc taccgcaggc agggaatcca ccagcgttcc
     4921 atcaagatca aacagaaaac ctttgcaccg cacgcgggcc tcctcaggcg ttgataattt
     4981 gattaatttc gttggcgctc aaatggtact gacgcgggca ggcatgccac acattaagca
     5041 tgcgctgata tttttcccac attggcgtct gggcgttaaa accatgagtc ccagcgtcaa
     5101 aatgggtata gcgcccttcc acattaacca taaagcggac ataaccgagg taacgtgctt
     5161 cagtggcggc gtcaaagccg aggaaggtga cacgacgttc atcgatggat tgctggtcct
     5221 gcaaattaga ccaggaaaca tacaacgcat gatacatatc cataatgtcg atgatagtgc
     5281 ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa ttcacgcatc tgtaatccgt
     5341 aaccacgctc aataattgtt tgcaggcgac ggtaacgttc agcatttgcc ggatcgagca
     5401 tagtcatcat cttgtactga ttagacaaaa taagacgttg cgcgttggtc atttccattg
     5461 ttgactcctg tatcactcta ctacggtgaa aaaaaagaag gctgagtatg ccttctttta
     5521 tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag ttttatccag ttgtttgaag
     5581 gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct tgccttcaac gggtgccaac
     5641 acctgtccag actcctgcaa tttaccgcga acttcataaa accagttaag gattgcaggg
     5701 ggtaatggcg ttacagaacg cttgcccagc caccacaatc cctgcatggg taaacttaag
     5761 gcgaacagtg cagtggcaac tgccggccca agcagaccgc ccagggcaat ctgccagcag
     5821 agagtaaata cggcgatcgg cggcataaaa cggatcgcat aacgcgtcat cttgataacg
     5881 cgattttcga caaagaccgg agcaaggcgt ttttccagtg gccacgtctt tgagtaatgc
     5941 tgtccgcggc gaaacaagct aaaaaaatta acagaacgat tatccggcgt tgacatgctt
     6001 cacctcaact tcacatataa agattcaaaa atttgtgcaa attcacaact cagcgggaca
     6061 acgttcaaaa cattttgtct tccataccca ctatcaggta tcctttagca gcctgaaggc
     6121 ctaagtagta catattcatt gagtcgtcaa attcatatac attatgccat tggctgaaaa
     6181 ttacgcaaaa tggcatagac tcaagatatt tcttccatca tgcaaaaaaa atttgcagtg
     6241 catgatgtta atcataaatg tcggtgtcat catgcgctac gctctatggc tccctgacgt
     6301 ttttttagcc acgtatcaat tataggtact tccatgtcga gtaagttagt actggttctg
     6361 aactgcggta gttcttcact gaaatttgcc atcatcgatg cagtaaatgg tgaagagtac
     6421 ctttctggtt tagccgaatg tttccacctg cccgaagcac gtatcaaatg gaaaatggac
     6481 ggcaataaac aggaagcggc tttaggtgca ggcgccgctc acagcgaagc gctcaacttt
     6541 atcgttaata ctattctggc acaaaaacca gaactgtctg cgcagctgac tgctatcggt
     6601 caccgtatcg tacacggcgg cgaaaagtat accagctccg tagtgatcga tgagtctgtt
     6661 attcagggta tcaaagatgc agcttctttt gcaccgctgc acaacccggc tcacctgatc
     6721 ggtatcgaag aagctctgaa atctttccca cagctgaaag acaaaaacgt tgctgtattt
     6781 gacaccgcgt tccaccagac tatgccggaa gagtcttacc tctacgccct gccgtacaac
     6841 ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg gcaccagcca cttctatgta
     6901 acccaggaag cggcaaaaat gctgaacaaa ccggtagaag aactgaacat catcacctgc
     6961 cacctgggca acggtggttc cgtttctgct atccgcaacg gtaaatgcgt tgacacctct
     7021 atgggtctga ccccgctgga aggtctggtc atgggtaccc gttctggtga tatcgatccg
     7081 gcgatcatct tccacctgca cgacaccctg ggcatgagcg ttgacgcaat caacaaactg
     7141 ctgaccaaag agtctggcct gctgggtctg accgaagtga ccagcgactg ccgctatgtt
     7201 gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa tggacgttta ctgccaccgc
     7261 ctggcgaaat acatcggtgc ctacactgcg ctgatggatg gtcgtctgga cgctgttgca
     7321 ttcactggtg gtatcggtga aaatgccgcg atggttcgtg aactgtctct gggcaaactg
     7381 ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg ctgcacgttt cggcaaatct
     7441 ggtttcatca acaaagaagg tacccgtcct gcggtggtta tcccaaccaa cgaagaactg
     7501 gttatcgcgc aagacgcgag ccgcctgact gcctgatttc acaccgccag ctcagctggc
     7561 ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag aggataaacc gtgtcccgta
     7621 ttattatgct gatccctacc ggaaccagcg tcggtctgac cagcgtcagc cttggcgtga
     7681 tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc gctcagccgc
     7741 gtaccggtgg cgatgcgccc gatcagacta cgactatcgt gcgtgcgaac tcttccacca
     7801 cgacggccgc tgaaccgctg aaaatgagct acgttgaagg tctgctttcc agcaatcaga
     7861 aagatgtgct gatggaagag atcgtcgcaa actaccacgc taacaccaaa gacgctgaag
     7921 tcgttctggt tgaaggtctg gtcccgacac gtaagcacca gtttgcccag tctctgaact
     7981 acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt tatgtctcag ggcactgaca
     8041 ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa cagcttcggt ggtgccaaaa
     8101 acaccaacat caccggcgtt atcgttaaca aactgaacgc accggttgat gaacagggtc
     8161 gtactcgccc ggatctgtcc gagattttcg acgactcttc caaagctaaa gtaaacaatg
     8221 ttgatccggc gaagctgcaa gaatctagcc cgctgccggt tctcggcgct gtgccgtgga
     8281 gctttgacct gatcgcgact cgtgcgatcg atatggctcg ccacctgaat gcgaccatca
     8341 tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt cactttctgc gcacgcagca
     8401 ttccgcacat gctggagcac ttccgtgccg gttctctgct ggtgacttcc gcagaccgtc
     8461 ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg cgtagaaatc ggtgccctgc
     8521 tgctgactgg cggctacgaa atggacgcgc gcatttctaa actgtgcgaa cgtgctttcg
     8581 ctaccggcct gccggtattt atggtgaaca ccaacacctg gcagacctct ctgagcctgc
     8641 agagcttcaa cctggaagtt ccggttgacg atcacgaacg tatcgagaaa gttcaggaat
     8701 acgttgctaa ctacatcaac gctgactgga tcgaatctct gactgccact tctgagcgca
     8761 gccgtcgtct gtctccgcca gcgttccgtt atcagctgac tgaactggcg cgcaaagcgg
     8821 gcaaacgtat cgttctgccg gaaggtgacg aaccgcgtac cgttaaagca gccgctatct
     8881 gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa tccggcagag atcaaccgtg
     8941 ttgcagcgtc tcagggtgta gaactaggtg caggcattga aatcgttgat ccagaagtgg
     9001 ttcgcgaaag ctatgttggt cgtctggtcg agctgcgtaa gaacaaaggc atgaccgaaa
     9061 ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg tacgctgatg ctggaacagg
     9121 atgaagttga tggtctggtt tccggtgctg ttcacactac cgcaaacacc atccgtccgc
     9181 cgctgcagct gatcaaaact gcaccgggca gctccctggt atcttccgtg ttcttcatgc
     9241 tgctgccgga acaggtttac gtttacggtg actgtgcgat caacccggat ccgaccgctg
     9301 aacagctggc agaaatcgcg attcagtccg ctgattccgc tgcggccttc ggtatcgaac
     9361 cgcgcgttgc tatgctctcc tactccaccg gtacttctgg tgcaggtagc gacgtagaaa
     9421 aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc tgacctgatg atcgacggtc
     9481 cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa atccaaagcg ccgaactctc
     9541 cggttgcagg tcgcgctacc gtgttcatct tcccggatct gaacaccggt aacaccacct
     9601 acaaagcggt acagcgttct gccgacctga tctccatcgg gccgatgctg cagggtatgc
     9661 gcaaaccggt taacgacctg tcccgtggcg cactggttga cgatatcgtc tacaccatcg
     9721 cgctgactgc gattcagtct gcacagcagc agtaatctcg tcatcatcca cagctttgcg
     9781 ctgcggatat ctgaaccgga aataatcact atttccggtt ttttattctc ttaatttgca
     9841 ttaatccttt ctgattatct tgcttaactg cgctgcatca atgaattgcg ccatctcact
     9901 ttgcatactt accactttgt tttgtgcaag ggaatatttg cgctatgtcc gcaatcactg
     9961 aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt ggtgattatc ttttttgttg
    10021 ctattttaac cagtcttgcc acctgggtag ttccggtggg gatgtttgac agtcaggaag
    10081 tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt cgtagatcca cactcatttc
    10141 gcattctgac taacgaagca ggcgaacctg agtatcaccg cgtacagctg ttcacgacgg
    10201 gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg gttaacctca ggatcgaaat
    10261 acgggacagc cgttggcatc atcatgttta tgctggtgat tggcggcgcg tttggcattg
    10321 tgatgcgtac aggaaccatt gataacggta tcctggcgct tattcgccat acccgcggga
    10381 atgaaattct ctttattcct gcgctgttta ttctgttttc acttggcggc gcggtatttg
    10441 gtatgggaga agaggccgtc gcctttgcca ttatcatcgc accgctaatg gtccggctgg
    10501 gctatgacag tattaccacc gtcctggtga cttatattgc cacgcaaatc ggttttgcca
    10561 gttcgtggat gaacccgttt tgtgtggttg ttgctcaggg gattgccggc gttccggtgc
    10621 tttctggctc cgggttgcgc atcgtggtgt gggttatcgc cactctgatt ggcctgatct
    10681 ttaccatggt gtacgcctca cgagtgaaaa agaatcctct tctgtcacgc gtgcatgagt
    10741 ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca acgtccgttt acctttggtg
    10801 actggctggt attgattgtc ctgacagccg taatggtctg ggtgatttgg ggcgtgatcg
    10861 ttaatgcctg gtttattcca gaaattgcca gccagttctt caccatgggt ctggtgattg
    10921 gcatcatcgg cgttgttttc cgccttaacg gcatgacggt taataccatg gcttcatcct
    10981 ttaccgaagg ggcgcgaatg atgatcgccc ctgccctgct ggtgggtttc gccaaaggga
    11041 ttttgctgct ggttggtaat ggtgaagcag gtaatgccag cgtgttaaat actatcctca
    11101 acagcattgc caatgccatt agcggtctgg acaacgcggt cgcggcctgg tttatgttgc
    11161 tcttccaggc tgtatttaat ttcttcgtga cgtccggttc tggtcaggcg gcattaacta
    11221 tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa ccgtcaggtt accgtgctgg
    11281 cgttccagtt tggtgatggc ttcagccaca tcatttaccc aacctcagct tcgttaatgg
    11341 caacgctcgg tgtgtgccgg gtggacttcc gtaactggct gaaggtgggc gcgaccctgc
    11401 ttggactgct gtttattatg tccagcgtcg tagtgatcgg cgctcagttg atgggctacc
    11461 actaaaaatg ttaagagccg caattgcggc tcttttttca ttctgccgtt tcagtctcta
    11521 cggcttcatt tttggcattg cgcttcatcc acaacgccag cgctttcagc gagtctggag
    11581 tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag ccagcaaact ccactgactt
    11641 catcttcctg tagggcgaag ggaccgtgag agacgcaact gaacaacgcg ccccagacac
    11701 ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc aaaggggaca ccggcaatgc
    11761 ccaactcttc ttccgcttcg cgacgcgcgg attccagcag ttgctcatcg gcctgaacta
    11821 caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc ttttgtctcg gtacgacgct
    11881 ggaccagaat tttgcccatg ccatcatgca cgacgatgta agttgcacga tgacgcagac
    11941 actgtgcccg catttgttcc cggctggctt gtgcaatgac ttcgttctct tcattgacaa
    12001 tatccaccca ttcagtactt gccaaacgac gctgttccat tatcaggaaa ccttcttttt
    12061 ctggcgctct tacggcgcat tttgagttgt gggtaaatta cggattaatc gcgacctgcg
    12121 caatgatact ttgatcattg agtgcgatta cactaagtac gtcattatcc agcatgccat
    12181 aacccgccgg attaccgcct ttcggaatac tcaccgagcc ggggttgaag tggaaaatct
    12241 ccccccgctg ttctgccact ggcagatggg tatgaccgta caccagcaca tcgttctggt
    12301 ttaaagcagg tagattttcc gggccaaaaa gatgaccatg cgtcaaaaac agacgttgtt
    12361 tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa atgcagcatc atttgatcca
    12421 cttcgctgtc gcagttgcca cgcacagcga taaccttatg tgccacttca ttaagccgtt
    12481 cagcgacttt ggctggcgcg taaccctccg gtaaagcatt acgcgggccg tgattcaaca
    12541 cgtcgccaag gatcaccagc cactgggcac cgctttggac aaacaactcc acaacacgtt
    12601 ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat cagtttcatc actcactcct
    12661 cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt gctatcagcc agattgcttc
    12721 gccgaaagtg caataaaacg ctggacagac gctcgctgcc actggaatgt cgcataatcc
    12781 accaggcgtt ctggcacctc atcgccatgt agtaccaggc ggttaatcat tagcgccaga
    12841 tcagtatcag caatgcacca ttcaccaaat aaattcggct gacccagtgc taacaaatgt
    12901 tctgccatcg cgaacagttt ctctgcactg gcttttccct cggccgttag tggcgctttt
    12961 ttcgcccccg caaagacaac atccgtcgga cgctcttcgc ggatgggcat cagatcgctg
    13021 cgcagccagg cctgaatctg tcgtgcacgc gcacgatttt ctaaatcaag cggataaata
    13081 cgttcccagg tcggtggcgc aaatcgatct tccagatact ccgcaatggc agaagattca
    13141 ctcaactcaa aatcatcgat ttgtaataac ggcacacggc gtgtctgacc gtaaccttgc
    13201 cacgtcggct gcaaatgttc accgctgtcg aggtcgatgg tcttgatatg aaacgacagg
    13261 cctttttcct gcaacgccac ccaggcggat aacacataag gggagaaaaa gtgggcatct
    13321 gaccaaagcg tgatagcggg tttactcatt atgtcctcgg caataaaggg ggctggctat
    13381 caaactatag agcctttttg tcactgtcac gtgacgaata ctcacgtaag tcgccgcgct
    13441 ttctatactt gtggtgttca gatgattaaa caaccggagc tgcaatgatc gatctctatt
    13501 tcgccccgac acccaatggt cacaaaatta cgctgtttct cgaagaagca gagcttgatt
    13561 atcgcttgat taaggtagac ttggggaaag gcggtcagtt tcgtccggaa tttttgcgca
    13621 tttcgcccaa caacaaaatt ccggcaattg ttgatcattc tcctgccgat ggcggcgaac
    13681 cgttaagcct ctttgagtct gctgccattt tgttgtatct ggctgagaaa acaggactct
    13741 ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca gtggttattc tggcaggtag
    13801 gcggactggg gccgatgctt gggcaaaatc atcattttaa tcacgcagcc ccccaaacca
    13861 ttccttacgc tattgaacgt tatcaggttg aaactcagcg tctttaccat gtactaaaca
    13921 agcggctgga aaactcgccc tggctgggag gcgagaacta cagcattgcg gatattgcct
    13981 gctggccgtg ggttaatgcc tggactcgcc tgcgaattga cctcgcaatg tatccggcag
    14041 tcaagaactg gcatgagcgg atccgttcgc gccctgccac cgggcaggca ctgctaaaag
    14101 cacaactcgg tgatgagcgt tcggatagtt aacagaaaca ggttctcgtg tattatttca
    14161 tcctaagtaa aacaacggag aacctgcaat ggcacaacct gccgctatta ttcgtataaa
    14221 gaaccttcgt ttgcgtacgt ttatcggaat taaggaagaa gaaattaaca accgtcagga
    14281 tattgttatc aatgtgacga tccactaccc cgccgataaa gcgcgcacta gcgaagatat
    14341 caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt attcagcatg tagagaataa
    14401 ccgtttctct ttgctggaaa aattaactca ggatgtgctc gatatcgcac gtgaacatca
    14461 ctgggtgacg tatgctgaag tggagatcga taaactgcac gcgctgcgct acgccgattc
    14521 ggtatccatg accttaagct ggcagcgtta atcgccatat cgggaggctg catgaacata
    14581 gtgatcaccg gagggacggg attaattggt cgccatttga ttccgcgttt gctggagctg
    14641 ggccatcaaa ttacggtagt gacgcgtaac ccgcagaaag ccagttccgt tctcggccct
    14701 cgggtgacac tatggcaagg gcttgccgat caaagcaacc tcaacggcgt tgatgcggta
    14761 atcaacctgg ccggagaacc gattgctgat aaacgctgga ctcacaagca aaaagagcgt
    14821 ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg atttgattaa tgctagcgac
    14881 acgccgccgt cggtactcat ttccggctcg gcaacgggct attatggcga cttaggtgaa
    14941 gtggtggtta ccgaagagga accgccgcat aacgaattta ctcataaact ctgcgcccgc
    15001 tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc gagtgtgcct gctgcgaacc
    15061 ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa tgctgccacc gtttcgtctt
    15121 ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg cctggattca tattgatgat
    15181 atggtcaacg gcattctctg gctgctggat aacgagctgc gcgggccatt taatatggtt
    15241 tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc tcggtcatgc gctgcatcgc
    15301 ccggctattt tgcgcgtccc cacaaccgcc attcggctgt taatgggcga atcttcagta
    15361 ctggtattag gtggacaacg cgcgctgcct aaacggctgg aagaagcggg ttttgcgttt
    15421 cgctggtacg atttagaaga ggcgctggcg gatgtcgttc gctgatgtgg tttacagcaa
    15481 acatccgcca gttaactccc tgtgttacag gattagtggc tttgcgcgat aagatcgtct
    15541 ggtgaaagtc gggtcaccat cataactaac tctctgtcta aacctctatc cagcatctcc
    15601 tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg cgccttcttc acgtaatctg
    15661 tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc gttctgttat ctcaccaata
    15721 aatgcacgga aacgctgggc atcccctgtt tgtaatacgt aattaaacag ggcttttagc
    15781 tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa tttggtcgac taatcccaac
    15841 agatcgcgct gacgaatatg tttctgaatt aactccaaca gcgccatttt gcggtgttgc
    15901 ataatctcgt catccggcac cacggtaata tccaccaacg gaaacgctga tgaatatatt
    15961 ttgcgggcta tagcaggctc ggcaaattca tcaagccagc agagtgaata aggataagga
    16021 cttctgcaac catgataaaa cagcatcggg atcaccaatg gaagctcttt atagcccgca
    16081 tcaagatggt tttgcattgc cgcaatggaa taacgcatca tgcgaaaagc cattaattct
    16141 tccggcttac tttggtgctc tatcactaca taaatataac ccactccctc ctgcgttttc
    16201 acagaccaca agaggtcgga ataatattgc cgcaggtctt catcaataaa actgtttggt
    16261 tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg gcgcgggaag atgaatatta
    16321 ataaaattcc gcgcggtgtc tggatggcgt aaaaaagatt taaataccgc atcatgcggc
    16381 gtggaagttg tcgatattgt catggcgttc cgtcacccct caaatttaag atgacgcgac
    16441 aataaccacg attaacaccc aatgcagcag gcagttttta tctttggaca gcgaattcag
    16501 aagtattttt agcggactgc aacttttaca tttcagcgga agctgactcg caaattcccc
    16561 ttgaaaatac tccggcagcg ttagccaccg gagaggaaat tatttcagcg atcccttaag
    16621 gaactgttgc agacgagggc tttgcgggtt gccaaataac tgctccggcg cgccctcttc
    16681 ttctattttc ccctgatgga ggaaaatgac gtgagtagaa acatggcgag caaagcccat
    16741 ttcgtgagtc actaccacca tggttttccc ctcttctgcc agttgctgca taatacgtaa
    16801 cacttcgcct accagttcag gatcgagcgc cgaggtaggt tcatcaaaca gtaaaacttc
    16861 tggttccatc gccagcgccc gcgcgataga aacacgctgt tgctgaccgc cggaaagatg
    16921 caccggatat ttcccctgcg cacgttcgtc tatcccgact ttcgccagat acttcaccgc
    16981 ccgctcgcgc gcttcctgct tgctcaggcc caacacctga atcggcgctt ccatgacgtt
    17041 ttccaacacc gtcatatggc tccagagatt gaagtgctgg aataccatcg tcaggcgtgt
    17101 gcgcagtaag cgcagttgat ttttatcggc gactttgagt tgaccgtctt tgtcgcgcac
    17161 cagattgatc gtctggccat tgaccacgat cgatccttca ctcggttttt cgaggaagtt
    17221 aatgcagcgc agaaaggtac ttttccccga tcccgacgat ccgatgatgc ttattacatc
    17281 tccggcattc gcttgcagtg agaccccttt cagcacttcg tgttcgccat agcgtttgtg
    17341 caaatcgata acgtttaatt tattctcgga catcgtattc tcagtgcgtt gaagaaggtt
    17401 tcacatgctg caaccagcgt ttttccgctc tgcgaaagag gctgatcagg acataagaga
    17461 tgattaaata gagcaccgcg gcaatgccga aggcggtaaa aggttgatac gtggcggcgt
    17521 taatatcgcg ggctattttc agcagatccg gcacagtggc cgtaaacgcc agcgcagtag
    17581 agtgcagcat caggatcact tcgttgctgt atgccggtaa cgcaatacgc agcgcagaag
    17641 gcaaaataat gcagcgatac attttaaagg tcgagaagcc ataggctctg gcggcttcaa
    17701 tttccccatg cggaaccgaa cggattgccc cagcaaaaat ctcggtagtg taagcgcagg
    17761 tgttaagcgt cagcgccagc acggtacagt tcaggccact gcggaagaaa gcgttaagga
    17821 attcggttcc cttaacaatc tcaagcgtgt acatgccgga atagaacacc agcaactgaa
    17881 catacagcgg cgtaccgcga aaaatatagg taaataacca gattggaaac tggatgtatt
    17941 tattactgga gacacgacca atcgccagaa acagcgccag gactccgcct atcactaccg
    18001 acaaaataag cagccacaga gtgatcgcca caccagtaaa gcgataaccg tcggtccaca
    18061 gcagcggttt ccagtattca tgtaagattt cgatcacagg tcagccctct tcacacccac
    18121 ggagtagcgg cgctcaagga acagcagcac accattggaa acggtggtga aaaccaggta
    18181 aatcacgcca cagacgatgg cgaaatagaa cggttcccag gtacttttgc ctgccagctg
    18241 cgtggctttg accacatctt ccaggccgag taacgaaacc agtgcggtag atttgaggat
    18301 cacctgccag ttgttgccaa tgcctggcag ggcgtaacgc atcatcgccg gaaacatgat
    18361 ccgccgaaac acttgcccgc gagtaaaacc gaacgccgtc gccgcctcta tatgcccttt
    18421 cggcactgcc ataaacgcgc cacggaacgt ttcggtaaaa taagcaccgt agataaaacc
    18481 gagagtgata ataccagcga ccatcggatc gatatcaatc tgcccgacgc ccatcgcctc
    18541 cgtcaccatg tttagcgcaa tctgcaaacc atagaaaatc agcagcatca acaccaagtc
    18601 cggcacgcct cgaatcagcg tggtgtaccc ttcgaaaatc agccccgaaa gccgattttg
    18661 cgagagctta ccgccagcgc caattaaacc gatgattaca gcgagcacta cagagctgat
    18721 agccagctcc agcgtgacga gcgcaccctg taaaataaca cctgaaaacc catacaacat
    18781 gctgcctgtc ctgtcgtgtg tggtgaatta ccgcctcttg tctccctccg gttacccgaa
    18841 gggagcgggc tttctttcac cgatggcgat taaccaccat aaacatcaaa atcgaagtac
    18901 tttttcgcta atttctcgta agtaccgtca gcgcgcattt cggcaaaggc tttgttcagt
    18961 gcctcgcgca gttcgttatc ttctttacgc aggcccatgc cggtccctac gccaaacagt
    19021 ttttcatctt taacagacgg accaccgaat ttgtaatctt taccgacggg ttgtttgagg
    19081 aaaccttcgc tggcagcgac ctcatcctgg aacgcggcat caatacgtcc ggcagtcagg
    19141 tcagaataaa tgttgtcctg cccctgatac gagacgattt caatgccttt tggtgcccag
    19201 tgttcattac cgaacgtctc ctgggtggtg ccctgcaata cgcctacccg tttgcctttc
    19261 agcgactcga ctgtcggctg aatgtcagaa tttttcgcca ccaccagacg agaatcggca
    19321 gcgtacagtt tgtcggtgaa ggcaatttct tgctgacgtt tttccgtaat ggaaagcgat
    19381 gacatgatgg cgtcaatctt cttcgctttt aaggacggga ttaacgcatc cagcgggttt
    19441 tcgacaaacg tacattgcgt attgatgcgc ttgcacaact ccttcgccag ctcgatatcg
    19501 aaaccaacca gttcgccttg tgaatttttt gattcaaatg gcgcataggt cgggtcggta
    19561 ccgatgcgga tgttttgcgg aattgcagca agtgccgcag ttgcgctgga gaaggccaga
    19621 accagagaga gcgatagcac cagttttttc ataatgtcct caactgacag tctttttata
    19681 gggaatattt acaggtttgt tacacttatc gtgccataaa attgaccatc aaggcaaaat
    19741 atcccgcccc gttagacgta ttatttttca ttaaaaatgc ttaagtatgc atttcaatgc
    19801 accacgatag tgcaacaaac ctggcgtgca ccatgatgat cattccatca ggtacagctt
    19861 cccagcgacg tatcagtcac cgtagacatt aaagtcgaaa tactttttcg ccatcttgtc
    19921 gtaggtgccg tcctgacgca gctcgccaag cgccttattg aaggcagccg tcagttcagc
    19981 ataatcttta cgtagcccta caccggtgcc atcaccgaag tattttttgt cttttactga
    20041 tgagccagca aaagcgaaat ctttaccagc aggttgcttg aggaatcctt cgctggcagc
    20101 aacttcatct tgtaacgcag catccagacg tcctgcagcc agatcggaat agaccaaatc
    20161 ctggttggca taggccacca catccacgcc tttactacgc caggtctcgt tagcgtaagc
    20221 ttcctgggtt gatccctgca gcacaccaac atgtttacct ttcagtgaat ccagcgttgg
    20281 ctgaatcggt gaacctttgg cagcaatcaa acgagaatct gcggcgtaca gcttgtcgga
    20341 gaaggcaatc tcctgctgac gtttatcggt aatggaaagc gacgaaataa tagcgtcgat
    20401 ttttttcgct ttcagtgagg ggatcagcgc gtcaaagtca ctggcaaccc aggtacattt
    20461 cacctgcatc cgtttgcaca tctcgttacc gagatcgata tcaaagccaa caaaatcacc
    20521 tttagcatct ttcgacgaga acagcgcata ggtggtatcg gttccgatac gtaccgtctc
    20581 cggtagcgcc gcatagctgg aagccgctgc ggagagaccg actaataaag acagagcgag
    20641 aatcgacttc ttcatacata accctcaagt gaaaatggct tttttatgtt gtgtattgtg
    20701 ttgtgtgttt gcaggctctt tcatgcaggt cttatgccat cttgacgaca acagtaacat
    20761 tcaacgttaa atatgttaat aagacattgc attattgtcc tgaagttgaa gatagcaggt
    20821 atagcggttg aatcgcagcg tttcgattga atggcagaaa caaaatgtcg aggatttgat
    20881 cgcggttgca aaattgccct gaaacagggc aacagcggag ttatgcgccc tgccagcggg
    20941 caaagagatc ttcaggaagg gttattgcaa actggtcaag aacacgatta accgtctgat
    21001 ttatcacatc atcaagggat tgcgggcgat gataaaacgc cggaacggga ggcataatca
    21061 ccgcaccgat ttctgccgcc tgagtcatta aacgcagatg gcctaagtgc aatggtgttt
    21121 cacgcacgca gagcaccaac gggcgacgct ctttcagcac cacatctgcc gcacgggtca
    21181 gtaagccatc agtatagcta tggacaatgc cggacagggt tttgattgaa cagggtaaaa
    21241 tcaccatccc cagcgtctgg aaagaaccgg aagagatgct ggcggaaata tcgcgcgcat
    21301 cgagcgtgac atcagctaat gcctgcactt cgcgcaggga aaaatccgtt tcgagggata
    21361 aggtctggcg cgccgcctgg ctcatcacca gatgcgtttc gatatctgtg acatcgcgca
    21421 gaacctgtaa taagcgcacg ccataaatcg cgccgctggc accgctgatg cctacaatga
    21481 gtcgtttcat aaatagttgc cctgtcagac ttgcaggcag actttgcagt atttcggcgg
    21541 gagttgcaag tcagggtgcc agaccggcac cctcagcgaa ggcatcatcc ttcgttatgc
    21601 atttcgagat tttccacttc gttctgacgt tgcactgctt tggcgtcatc attacgtaac
    21661 gtatcgagga aatcgaggta gccctgatca acatctttgg tgacgtagac gccgttgaac
    21721 accgagcatt caaactgctg gatatccgga ttttcagcgc gaacggcgtc gatcagatcg
    21781 ttcagatcct ggaaaatcaa cccgtcagca ccgatgatct ggcgaatttc atcaacttcg
    21841 cgaccgtgag cgatcagttc cgtggcgctc ggcatatcaa taccataaac gttcgggaag
    21901 cgaatttccg gtgccgcaga agcgaggtac actttcttcg ctccggcttc gcgtgccatc
    21961 tcgataatct gctcagaagt ggtgccacgg acgatggagt cgtcgaccag caggacgttt
    22021 ttatcgcgga actcggcgcg gttggcattc agtttacggc gcacggactt acgacgcagc
    22081 tgctggcccg gcatgataaa ggtgcggcca acatagcggt ttttaacgaa gccctggcgg
    22141 tacggtttgc ccagaatacg agcaatttcc agcgcgatat cacacgaggt ttctgggatc
    22201 gggatcacca cgtcgatatc cagatcttcc cattcgcggg caattttctc gcccagtttc
    22261 gtgcccatat tcacacgcgc gctgtaaacg gaaattttgt cgataaacga gtccgggcgg
    22321 gcaaagtata catactcaaa caggcacgga ttgctgaccg gattgtcagc acattgacgg
    22381 gtaaacaact gcccttcttc agtgatgtaa atcgcttcgc ccggcgcgac gtcacgcagg
    22441 aaatcaaagc ccagcgtatc gagcgctacg ctttcggaag cgaccatata ttctgtacgg
    22501 ttctcgtcaa tatcacgttt tcccagtacc agcggacgaa tcccgtttgg atcgcggaaa
    22561 gcaaccatac cgtggccgat aatcatcgcc acacaggcat acgcgccgcg gattaagcgg
    22621 tttgtggcag caatggcagc gaaaatattg tcggcttcca gcgggtagtg gcggaagttg
    22681 tccagctcgc tggcgaagat attaagcaga atttccgagt cggaagtggt gttgatgtgg
    22741 cggcgttttt cttcaaacag ttttttacgc aactcgtgag cgttggtcag attgccgttg
    22801 tgggcaagcg taatgccata cggggagtta acgtaaaacg gctgcgcttc agaggcgctg
    22861 gagctgccag ccgtagggta acgcacatga ccaatgccca tattgccctg caaacgctgc
    22921 atatggcgag cttcaaatac atcgctcacc agcccgttcg ctttacgcaa acggaagcag
    22981 ttattggcat ctatggtgat gatgccggcg gcatcctgac cgcgatgctg aagcaccgtt
    23041 aaggcatcat aaatcgactg gttaaccggc ataacaccgg cgataccgac aataccgcac
    23101 atacgtcttt ttcctcgtta agccacatct cagagcactt acgctctggg caagaaactt
    23161 gacgagcttt gcagataatc aaaaaaccat ctgatgataa aactgaattg cgggatcagc
    23221 tgtgatttgc tccagtcttc acttttcgac accccggtaa aggagtcgag aaagaagaga
    23281 atggcggcaa caatcaacac accgcgcaac gcaccgaaac agacgcccag cacccgatcg
    23341 gtgcctgaca accccgtttt ctccaccaac tggcctatca cgaagttcac gatagcacca
    23401 acgatcaggg tagcgataaa cagtaccgcg atggcaatcc catttcgaac cagttcgtct
    23461 tcaaagcccg taaaccagac tgacaggtaa gtgtagtaat gactggcaac aaagaaagca
    23521 caaccccatg tcaccagcga taacgcttca cgaacaaagc cgtggatcag gctaaccaga
    23581 gaggaaaaag caatcaccgc gattatggcg taatcaatcc agaccatatg tgtcccacga
    23641 ttttacgccc tgtcatcctg ttcggggcgc attctaacag aaaaagaaaa cgtttgcgta
    23701 gggatttcct tcccgcgcat caataaaaat ggcgctgaaa aaatattcaa cgccatcgac
    23761 tttttatgcc tttgcggcat cgtgcaatgc gtgtcggatg cggcgtaaac gccttatccg
    23821 acctacggtt ctaacactgc ccaagcctga taagacgcgc cagcgtcgca tcaggcaaga
    23881 ctgtattaat tcggcgtata gcccattacc acgccactta agccagaaag ttgcttcaac
    23941 tcacccagcg aacctttcag cttatctttc gaggcatccg gcccaaccag aatacgggta
    24001 attttaccct gcactggcgt ggatggcgac gtataaaccc gataaccggc accgcgcagc
    24061 ttaccgacaa tctcattcac tttatcggca tttttcagcg cacccagttg cacaacatag
    24121 gctttacccg ttggcgctgc tttttcttcc acgaccggct tcggttctgg cttcggcgca
    24181 ggtggtactt caacctttgg tttaggcggc tccaccggtt tcggcttcgg tggggcgacc
    24241 ggtgcaggtt ccggttcaaa ctcggtgtta ttggctgcaa tagtggccgg atcgagcgac
    24301 ggtgctgcgg catcacctgc ccgcacctct tccgctgcgc cttccggcgg ctgcgtcggc
    24361 aacgcctggg tggcggctgg catcatatca ggctcatcac gatcgcccgc tttcggcacc
    24421 agcgggatag ccgcgaactc atcctgataa tgttttttct gcccgtccag cagccctgga
    24481 agtacaatca cccccagcgc caccagcacg atcgtgccca ctaaccgatt ctgaaactta
    24541 cttgccaccg cttctcctcg cgtcaatcac ttccatgaca tgtgcgaccg tgtggaaaga
    24601 accacacacc agcacggtgt cttccgcttt agcgtccgcc attgcggcat cccatgcctg
    24661 cgcaacgcta tcaaatgatt tgccgttacc caaatgctca agcagttgtt ctgccgtggc
    24721 accgcgcggc ccttccagtg gcgcacaata ccagtcatca accacgcttt tcaaccaggc
    24781 cagagttccg gcaatatctt tatcatgtag cataccgata accgccagca cgcgcccgtt
    24841 tttcggtagc gctttcatac gcccggtgag atattccgcc gcatgtggat tatgcgcgac
    24901 atcaaaaata acgcgtggcg actcgctcac aatctggaaa cgtcccggca aaattgcgct
    24961 ggcaatcccg tcgcgaatgg cattttcact gacttccagc ccgctggcac gcagtgccgc
    25021 cagcgccgtt gcggcattcg gttgcgggac aagcggcaac ggcagatttt ccagcgtgcc
    25081 gtgagcatcg ctaaacgccc agtcatgatc ggtgacggaa tagttccact caacgccccg
    25141 acgttgtaac agtgcacctt tttcctgcgc cacatcagca atggtagaag gcatttccgg
    25201 ctcaccgaca attgccggtt tttcgctgcg gaagatgcct gctttctcga gaccaatact
    25261 ttcgcgatct ggacccagcc agtcggtatg atccagcgca atactggtta ctaccgcgac
    25321 atcggcatcg acaatattgg ttgcgtccag acgaccgccc agccctactt ccagaatcac
    25381 cacgtcaagt tgtgcctgct tgaacagcca caacgccgac agcgtaccgt actcgaaata
    25441 ggtcagggaa atatcaccgc gtgccgattc aatctccgca aaagaggcgg tgtgggccga
    25501 ttccggcaat tcctggccct gcacacgtac gcgctcggta taacgcacca gatgaggcga
    25561 actgtagacg cccactttgt accctgccgc catcagaatc gactccagcg tacggcaggt
    25621 ggtgcctttg ccattcgtac ccgcaacggt aaacacaaat ggcgctggtt tcaggacgcc
    25681 aagacgcgcc gcgacctggc tcacgcgctc aaggccgaga tcgatagttt tactgtgcag
    25741 gttttccaga taagaaagcc acgaagccag aggcgacgcg gcttgaggag tgcgtttgat
    25801 aatcatggta tccgctgatt cgttacggtg agaatagcaa aagggcagag ccagtggccc
    25861 tgcccttatc agttatcagg cctcaggttc ctgatccggt accgggggta ccactacgcc
    25921 ttcacgcggc gcttcaggat tcggcgctgg cagattcatc aacttcgcca gaatgctcgc
    25981 cagtttcagg cgcatttccg gacgacggac gatcatgtcg atcgcgcctt tctcgatcag
    26041 gaattcactg cgctggaatc taggcggcag tttttcgcga acggtctgtt cgataacacg
    26101 cggaccggca aagccgatta acgctttagg ttcagcgatg ttgagatcgc ccagcatggc
    26161 gaaacttgca gaaacaccgc ccatcgtcgg gtcggtcagc acggagatgt acggcaagcc
    26221 gcgctcctgc atttttgcca gtgccgcaga ggttttcgcc atctgcatca gcgacatcag
    26281 tgcttcctgc atacgtgcgc caccagaggc ggagaagcag atcagcgggc agttatcttc
    26341 ctgcgcctgc tcaacggcac gcacgaaacg tgcacccaca acagacccca ttgaaccgcc
    26401 cataaaggcg aactcgaatg ccgcagcgac aaccggcatt ccatacagag tgcctttcat
    26461 caccaccagc gcatcttttt cgccggtttc tttctgcgca gatgccagac ggtctttata
    26521 cttcttggag tcacgaaact tcagctcatc tttcggctca agctcgctac ccagctccac
    26581 aaggcttcct tcatctaaca ggctatgcag gcgattacgc gctgtcatac gcatgtgatg
    26641 gtcacacttc ggacagacct caagattacg ttccagctca gcgcggtata aaacctgacc
    26701 gcagctatca cacttagtcc acaccccttc aggaatgctc gccttgcggg tgggagtaat
    26761 gttgctttta attcgttcaa tccagctcat tagggacctt tctgtctgaa cctggttcga
    26821 tgccagtttt atctttgggg acgcataatg ccatttttgc ccccaacaga ccatgaatgt
    26881 tgcacattaa aacataacag cccgaaactt tggataaaaa agtggtcgaa ccgcggagtt
    26941 actttttatt ttgcggcgcg tgccgcagcg cgtttgtgac ggattatttc gatgacgccc
    27001 ggcaaaatag aaaccacaat aatcccgacg atcagcagct taaggttatc ctgaaccatc
    27061 gggattgtac cgaagaaata gcctgcgtag gtaaaaagca gtacccacaa cagtgcgccg
    27121 atcacgttat aagcagcgaa atgacggtac gacatgtggc ccattcccgc aacaaacggg
    27181 gcgaacgttc tgacgatcgg cacaaaacgg gcgagaataa tcgttttgcc gccatgtttc
    27241 tcataaaact gatgggtttt gtcgagataa ctgcgacgga aaattttcga attgggatta
    27301 ctgaataact tttcaccgaa caaccgcccg atagtgtaat tgaccgcgtc acccacaatc
    27361 gcggcgatca gcatcagcac taccatcata tggacgttga gatcgttggt ttccagtgat
    27421 gccaacgctc cggcaacaaa cagcaatgaa tcacccggta aaaacggcat taccaccaga
    27481 ccggtttcac agaacaaaat taagaacaaa atggcataaa cccagacgcc gtactccgcg
    27541 actagctctg ccagatgcac gtcaatgtgc aggataaaat cgatgaggaa ataaatcagg
    27601 tccatatttg cctatgcctt gtactcgtca tctttcaggc tgtaactacg ttggctacag
    27661 cctgaaatgt tccgagcatt attctcgtta gtccgccaga aatagcgggc ccattggcgg
    27721 ttttggaaga tcataccggt cagggtaatc caccgcgacc agatacagcc cttccgcttt
    27781 tgccgttgct gccgccagcg ttctgtcctt tgccgccagc aactctgcta tccagctctc
    27841 cggctggttg tgggcaccga cttccatcag gctgccgaca atattcctga ccatatgatg
    27901 tacaaaggca ttcgctttga tatcaaccac cacataagga ccgtgacgcg tgacattaat
    27961 atgcataacg ttgcgccacg gggttcggga ctggcactgc accgcacgga acgaggtgaa
    28021 atcattctcg cccagcaagc attgcgcagc ccgatgcatc cgttcagcgt ccagcggttc
    28081 gtaaaaatgg gttacccctt tactcagtac cgccgggcgc agccgatgat tgtagatgat
    28141 gtagcgataa cggcgagccg tggcgctaaa tcgggcatga aaatcatcag gtacagcttt
    28201 aacccaacgc acggcgatgt caccaggtaa attcgcattt acgcccaagg tccacgccgc
    28261 gtctttgcgc tgcgcggttg tttcgaaatg caccacttgc ccggtaccgt gtacccctgc
    28321 gtcagtacgc ccggcgcaga agacggtgat gggttcgttc gccacctggg agagcgcctt
    28381 ttccagcttc tcctgcacac tgcggacttc attctgccgt tgccagccgt aatacttact
    28441 gccgtcgtac tcaatgccca gcgcaatttt ataaactggc agttgttgct ggtcggacat
    28501 tagtacagat actcctgcac cagtttctcg gcgattttta ctgccatcag cgcgccgcca
    28561 aagcgaacgt tatcggccac cgaccagaac tggacttgct ccggcatacc gtagtcatta
    28621 cgcacgcagc caacagaaag atgcggcgta cccgaagcat cccctacctg agtcgggaat
    28681 tcgttctctt cagagagcac aatatcttcg ccctgagcaa acgcatcacg cgcttcttct
    28741 gccgccagcg gacgcagtgc ttcaaagttg accatctggg catgaccgta gaataccggt
    28801 gcctggacga cgcttgccga aatcatcagc ccttcgtcct gcaggatttt gcgtacttcg
    28861 tcaacgatac gacgttcttc acgcacgcta ccttcgctat ccggcaacaa cggcagcata
    28921 ttgaacgcca gttgacgacc gaagaaatct tcttcgtcaa tcggaatgcc gttgagcaat
    28981 ttcgcactct gccccgctaa cgcatcgacc gcttttttgc cctgggcgga ggctgaaatc
    29041 aggctggtaa cactgatacg cgacaaaccg ccctgatcga ttaacggttt cagtgccgcc
    29101 agcagctggc tggtcagact gtctggtacg gcgatgacat tccggttgcg gtaatcggtc
    29161 agtacaaacg ggtttacttc cggcaccacc agcggtacgt cgggttcgag agcaaacagt
    29221 ccgctgctgt cgatcaccag gcaacctgag ttggtcgctt cttcaaccca ggcagcggta
    29281 gcttctttgc ctgcgacaaa aaatgccagc tgcgcctgcg tccagtcgaa ttcagcggca
    29341 tcctgcacgg tgattgtttt accaccaaag cgcagttgtt cgcctgcgct ttcgttacgt
    29401 gccagtgcat aaatttcccc aaccgggaac tgacgttcag ccagcgtttc aagcagggct
    29461 tcgcccatag cgccagttgc gcccaggacg gcaatgttcc agccttcaga catggtggtt
    29521 tactccagaa atagcaaagc tccctgccaa tgttacagca gagagcatga agaagagatt
    29581 aacgtgccgg atgatgaacg gcgttaaaac ccagtttaca cagcaatgat gccgcactgg
    29641 cgtcatcaca aattacatac agagatgacc attcacggcg ctcaagatag tttttgcgca
    29701 gtttatcgaa ctcacccggt atcccggcga ctttacgcag cggtgcgtca tcgcggcgca
    29761 catcatacac caaatgcacc agccttttca gcgtcggttg atcgagcgga ccatgcagcg
    29821 taatgcggcc aaactcaggc gcgggcagca acgtatccag cgcaacgtgt tgttcatgcc
    29881 caataaactt gctataggct tcaaacactt gcgtcgtgcc acgcgcttta ccctccaggg
    29941 tatagcctgc gatatgcggc gtgccgatat ccacttttgt cagcaattcc acattaagtt
    30001 ccggctcgcc ttcccagaca tccagcacca cgcttaactt ctggccttca ttcaggcagg
    30061 tcagcagcgc ggtattatcg acgactgcgc cacggcaggc gttaatcaga atcgctccgg
    30121 gcttcaggct acggatcagt ttttcatccg ccagatgtag cgttttgtac ggaccatctt
    30181 taaagagtgg cgtatggaaa gtcagaatat ccgcgcgctg gactaactca tccagcgagc
    30241 ggaaatcacc ctcatcccca cggtcggcgc gaggcggatc gcaaagtaag gttttaatcc
    30301 ctaacgcttc cagtcgcgcc tgcaatcgac gtccaacgtt accaacgccc acgatcccca
    30361 ccgtacggtc gtgcagtgaa aatccatcgc gttcggcaag catcagcagg gaggagaaaa
    30421 catattccac caccgcaatc gcattacagc caggtgcagc ggaaaaacca attcccgcct
    30481 gcttcaacca tgcttcatcg acatggtcgg tccccgcagt ggcagttcca acaaatttaa
    30541 tgggttttcc tgccagcaaa gattcattca ctttcgtgac cgaacgcacc atcagcgcat
    30601 ccgcgtctgc cagttgagcg acggggattg gacgcccggg aaccgcgatc acctcaccca
    30661 aacggctaaa taagtcgcgg gcataaggca tattttcatc aacaaggatt ttcacgtttg
    30721 tgttacctgt atgagacgag agttaaccgg acaagtatgc cataatctcg cggccaggca
    30781 tacttgcgaa gatttcaggt ataaggatac gtaatgatac aacctatttc cggccctcct
    30841 cctgggcagc caccaggtca gggagataac ctgccgtctg gcgcgggcaa tcagccttta
    30901 tccagtcagc aacgtacttc gctggaaagc ttaatgacga aagtgacctc actgacgcaa
    30961 caacaaagag cagaactgtg ggcgggtatc aggcacgata ttggtctgtc gggagattca
    31021 ccgctgcttt cgcgtcactt ccctgccgct gagcataatc tggcgcaacg tctgctggcc
    31081 gcgcaaaaaa gccattctgc ccgccagctt ttagcgcaat taggggagta tttacgtctg
    31141 gggaatcatc gtcaggcggt cacggattat atccgtcata actttggtca gacgccgctg
    31201 aatcagctct caccggagca attaaaaacc attctcaccc tgttgcagga agggaagatg
    31261 gttattccgc aaccacagca gcgcgaggcg accgaccgtc ctttattacc ggcggagcac
    31321 aatgcgctaa aacagctggt gaccaaactt gcggcggcaa cgggggaacc cagcaaacag
    31381 atctggcaat cgatgctgga actttccggg gtgaaagatg gcgagttaat tccagcgaaa
    31441 ctgtttaacc atctggtgac ctggctacag gcgcgtcaga cgctaagcca gcaaaatacg
    31501 ccgacgctgg aatcactaca gatggcgcta aaacaacctt tagatgccag tgaactggcg
    31561 gcgttatcgg catatatcca gcaaaaatat ggcctttctg cgcaatcatc gctttcttct
    31621 gcccaggccg aggatattct taatcagctt tatcaacggc gggttaaagg gattgatccg
    31681 cgtgatatgc aaccgctgct taatcctttt ccaccgatga tggacacgtt gcaaaatatg
    31741 gcaacgcgtc ccgcgctgtg gatactgtta gtcgcgatta tcctgatgct ggtctggctg
    31801 gttcgttaac cccgacgaaa tgacagtatc gtgacaataa tacccagcac cgcagagatc
    31861 gccccggcaa gaaataccga agagtaacca aacgtggtcg ccagcattcc cgccagcggc
    31921 ccggagacgc cgagggcgat atcctgaaac gcggcgtaac cgcccagtgc ggtgccgcga
    31981 acttgtgagg ggacgcgttt aaccacctcc acgcccagcg caggaaagat aagcgaacat
    32041 ccggctccgg ttaacgccgc gcccgctaat gcgacccatg cacctggggc ttgccagagc
    32101 agcaacaagc ccaccgtttc tacaagcaga gagacaatcg ccactttcac gccgccaaaa
    32161 cggtccggca tccagccaaa catgacgcgc atcacgacaa atgcgccgcc aaacgcggta
    32221 agagtaaagc ccgccatcgc ccatcctttg ctggcaaagt agagcgaaac gaaagtcccg
    32281 ataaccgcaa aaccaacgcc ttgtagtgcc agccctaacc ctggtttcca gataagcccg
    32341 acaacgctcc acagcgatgg acgttctccc gccagggccg gtactttgcg cactgtgccg
    32401 ttacaggccc acgccagtaa gggtaatacc attgtggtga tcgccagtgc ggcaaaaccg
    32461 taatggctat gaatcaacag gccaagcggc gcaccaacag cgagggcacc gtaaatcgcc
    32521 attccgttcc atgacatcac tttgccagag tgttttggcc ctacgatgcc taacccccag
    32581 gtcagagcgc ctgtcagtaa ctggctttca ccaaacccaa gaatcaaacg cccgatgacc
    32641 aacagggcaa atttgaacgg tgcggagaca ggcaaaatcg ccgccagcag caacgcgccg
    32701 ccagccagac cacaagctaa catcccctga agcgccgaac gttttgcgcc atattgatcg
    32761 gccagtcgcc cggcgtaacc acgcgtcagc accgtagcca gaaactgaat cccgacggca
    32821 atgccgacca tggtattgcc atagcccaga tcatgatgaa caaacagcgg gataaccggc
    32881 aacggcaacc ctacggtcat gtaggtgaga aaaaccgcaa aagagatgcg gaagagcgaa
    32941 aaactggcag aagatcgtgt ttcggtttgg cttacagcag tcatgcatta ctccagaatg
    33001 cagcgcaagg cgaggagtat ccccgtctca tctctctggt ttcagggtta cggtgcgttg
    33061 gcaggattta acgcgtacgt cttttcagaa ggaaatcgac aaagcgggaa gtttgcctag
    33121 aactggtggc gattgtcaat gatgtgaaaa agggaaccat caggttccct tttgcgttag
    33181 tgccggaggc cgcattggcg cgtaacgtcg gatgcgacgc tggcgcgtct tatccgacct
    33241 actccggcct actgcgaatt aatcttttca gcttgcgcat taccagcgtg gcgttggtgc
    33301 cgccgaagcc gaagctgtta gacataacag tggtcagttc gcgatcggtc gtttcggtca
    33361 cgatgttcag acccgcagcc tgctcgtcca gctcttcaat gttgatgctc ggggcgataa
    33421 agccgtgttc cagcatcagc agagagtaga tagcttcctg cacacccgca gcgcccagtg
    33481 agtgaccggt catggctttg gttgcagaaa tcgccgggct cttatcgccg aacacttcac
    33541 ggatagctgc cagctctttc acgtcgccaa ccggagtcga agtaccgtga gagttcaggt
    33601 aatcgattgg ggtatcaacg ccatgcatcg ccatctgcat acaacgtact gcgccttcgc
    33661 cagacggagc aaccatgtct gcaccatcag aggttgcgcc gtagccaacg atttcagcat
    33721 agatgtaagc accacgcgcc agcgcgtgtt ccagctcttc aaccactacc ataccgccgc
    33781 cgccagcgat aacgaaaccg tcacggtgag cgtcgtaagt acgggaggct ttttccgggg
    33841 tgtcgttgta tttagtagat agcgcaccca tcgcgtcgaa ttcgcaggcc atttcccagc
    33901 acagctcttc gccgccgcca gcaaacacga tgtcctgttt gcccagttgg atctgctcta
    33961 ctgcgttacc gatacagtgt gcggaagtcg cacacgcgga gctgatggag tagttaacgc
    34021 catgaatttt aaacggcgtg gcgagacagg cagaaacgcc ggatgccatc gctttagtga
    34081 ccacatacgg gccaaccgct ttcaggccgc gcgggccgcg cattgcgtca gcgccgaaca
    34141 cctggaaacg cggggaaccg ccgccggaac ctgcaatcag gccaacgcgc gggttattct
    34201 ggtaagcttc cggagagagg cccgcatcag cgattgcctg ctccatagaa aggaatgcat
    34261 aaatggatgc gtcgctcata aagcgcacaa ctttgcggtc aatgaggcca gtggtatcca
    34321 gttttatgtt gccccagacg tggctacgca tgccggaatc cttcagctcc tgagagaaag
    34381 tgatccctga acgtccttca cgcagagatg ccaggacttc ctgctggtta ttaccgatgc
    34441 tggaaacaat gcccaggcca gtaatcactg cacgtttcat tcaatacctc tgtaagtcgc
    34501 acatagagta agtttcgaat gcacaatagc gtacacttgt acgccgaaca agtccgatca
    34561 gccatttaat agagatattt gcgcagcctt acacacatcg ctaagatcga gccaccgcct
    34621 gtaagacgag taacttacgt gaaacactac tccatacaac ctgccaacct cgaatttaat
    34681 gctgagggta cacctgtttc ccgagatttt gacgatgtct atttttccaa cgataacggg
    34741 ctggaagaga cgcgttatgt ttttctggga ggtaaccaat tagaggtacg ctttcctgag
    34801 catccacatc ctctgtttgt gatagcagag agcggcttcg gcaccggatt aaacttcctg
    34861 acgctatgga aggcatttga tcagtttcgc gaagcgcatc cgcaagcgca attacaacgc
    34921 ttacatttca ttagttttga gaaatttccc ctcacccgtg cggatttagc cttagcgcat
    34981 caacactggc cggaactggc tccgtgggca gaacaacttc aggcgcagtg gccaatgccc
    35041 ttgcccggtt gccatcgttt attgctcgat gaaagccgcg tgacgctgga tttatggttt
    35101 ggcgatatta acgaactgac cagccaactg gacgattcgc taaatcaaaa agtagatgcc
    35161 tggtttctgg acggctttgc gccagctaaa aacccggata tgtggacgca aaatctgttt
    35221 aacgccatgg caaggttggc gcgtccaggc ggcacgctgg cgacatttac gtctgccggt
    35281 tttgtccgcc gcggtttgca ggaagccgga ttcacgatgc aaaaacgtaa gggctttggg
    35341 cgcaaacggg aaatgctttg tggggtgatg gaacagacat taccactccc ctgctccacg
    35401 ccgtggttta atcgtacggg cagcagcaaa cgggaagtag cgattatcgg cggtggtatt
    35461 gccagcgcgt tgttgtcgct ggcgttatta cggcgcggct ggcaggtagc gctttattgc
    35521 gcggatgaag cccccgcgct gggtgcttcc ggcaatcgcc agggggcgct gtatccgtta
    35581 ttaagcaaac acgatgaggc gctaaaccgc tttttctcta atgcgtttac ttttgctcgt
    35641 cggttttacg accaattacc cgttaaattt gatcatgact ggtgcggcgt cacgcagtta
    35701 ggctgggatg agaaaagcca gcataaaatc gcacagatgt tgtcaatgga tttacccgca
    35761 gaactggctg tagccgttga ggcaaatgcg gttgaacaaa ttacgggcgt tgcgacaaat
    35821 tgcagcggca ttacttatcc gcaaggtggt tggctgtgcc cagcagaact gacccgtaat
    35881 gtgctggaac tggcgcaaca gcagggtttg cagatttatt atcaatatca gttacagaat
    35941 ttatcccgta aggatgactg ttggttgttg aattttgcag gagatcagca agcaacacac
    36001 agcgtagtgg tactggcgaa cgggcatcaa atcagccgtt tcagccaaac gtcgactctc
    36061 ccggtgtatt cggttgccgg gcaggtcagc catattccga caacgccgga actggcagag
    36121 ctgaagcagg tgctgtgcta tgacggttat ctcacgccac aaaatccggc gaatcaacat
    36181 cattgtattg gtgccagtta tcatcgcggc agcgaagata cggcgtacag tgaggacgat
    36241 cagcagcaga atcgccagcg gttgattgat tgtttcccgc aggcacagtg ggcaaaagag
    36301 gttgatgtca gtgataaaga ggcgcgctgc ggtgtgcgtt gtgccacccg cgatcatctg
    36361 ccaatggtag gcaatgttcc cgattatgag gcaacactcg tggaatatgc gtcgttggcg
    36421 gagcaaaaag atgaggcggt aagcgcgccg gtttttgacg atctctttat gtttgcggct
    36481 ttaggttctc gcggtttgtg ttctgccccg ctgtgtgccg agattctggc ggcgcagatg
    36541 agcgacgaac cgattccgat ggatgccagt acgctggcgg cgttaaaccc gaatcggtta
    36601 tgggtgcgga aattgttgaa gggtaaagcg gttaaggcgg ggtaatctgc tctcgcagtg
    36661 tttgccggat gcggcgtgaa cgccttatcc aacctacgtg tgacgtgtag gcctgataag
    36721 acgcgataag cgtcgcatca ggcatggtgc tccagacgcc gcaacttaca gctgtgacgc
    36781 ctgctgaaac aggttttccc acatatcggt caccagcgcc tggtcacgcg gcgacaattc
    36841 accggcacca atggcttttt ccagactctg gctaacggtc gtatgcaccg cctgagcgga
    36901 gtggtcatca ccactttcca gttctgcgat ggctaatgtc aggtggccac gcaaataccc
    36961 actggcaaac aactcatcat cactggcatg gtcaaccatg ccgtcgatta atgccagaat
    37021 gcgtgattca aactccgcga tcatcttctt tcctcattgt aaaacgtggc gctggcttca
    37081 gttgagcgct tccggccacg ggaactgttc cgccgtaagt tccggcgtgt gataataatt
    37141 ttgtagtgct ttaatgaagc gtgccgggcg ttccggaatg ccatttgtca gataatccat
    37201 cacctgcgca tgtacccgcc gttgaaacac cacacgatcc ggttcgaaat ccccttccag
    37261 attgtcgcaa ctgacgttaa acggatatcc agccgccacg cagaataacc agtcgaacgc
    37321 ctgcggcttc acttcaacat cttcaaactg gctttgcgtc tgggcatcgc gtccgtccgg
    37381 gcaataccag tagccgaaat caaccagctc acggcgcgct tttccggcaa tacaccagtg
    37441 cgaaatctca tgaattgcgc tggcataaaa gccatgagca aagacgattc ggttatacgg
    37501 tacttccgca tcagcaggaa gatagatcgg ttcgtcgtcg cctttaatca gacgggtatt
    37561 aaattcatcg gcaaagcagc tattaaaaat ttcaatcaac tgctcgtagt ggtgtgtact
    37621 gttcattagt tcatccccaa ccagtggagg atctcctgtc cgtggctatc gtaaagtaat
    37681 ttggcactca tcaccgccga gacgataaca atcatcgggc ggatcagctt ttgtcctttg
    37741 ctcaacacca accgtgaacc catgcgcgcg ccgaggaact gccccaccag catgacaaag
    37801 cccgtcgccc aaatcacttt gccgccgaga ataaacagca gcagaccgcc aatgtttgac
    37861 gttgcgttga gtaatttggc gtgagccgtg gctttggcga ggttgaatcc gcacagcata
    37921 acgaaggcca gcgcgtaaaa cgatccggca gccgggccaa agaatccgtc ataaaaaccg
    37981 acgcagccac cggcaattaa cgcgaagggt aaaccgtaca tccggcgctg gcgatcttct
    38041 tcacccagct ttggcatcag caagaaatag aggccgatac aaatcaccag aatgggcaaa
    38101 atctgccgta agacatcagc ctgaacgtac tgtaccaaca gtgcgccgct cattgagccg
    38161 acaaaggtca tggcaatatt gagtttctga tcgcttaaac taaccacttt gcggcgaata
    38221 aagtagatag tggcggaaat agagccgccg caggcttgca gtttattggt tgccagcgcg
    38281 ttagcgggag acattcccgc agccatcaat gccggaatgg tgagtaaccc accgccaccg
    38341 gcaatcgagt cgataaatcc cgccagcatg gcgacaaaaa agagaactcc cagcaacagc
    38401 ggagaaacca taaacaggct attaaacgtt tccattagat cacgtgctca tccagtagcg
    38461 cctggcagga aggcggcaac ggaggcggtg tcttcttctc aggctttgtt gttcccggtt
    38521 ttggaggttc aaaccagctt tgcagttctg ccccgcaacc atcgcctgat ggcggtaaag
    38581 gttgatcttc acactccaga ctatcggcag ggcaacgtaa tcgtacatgc atatgcgcgc
    38641 gatgctggaa ccagggtcgc actttgcgca accagtcgcg atcggtgccc gcatcaaggc
    38701 aaagttgttg tttaatcgcc ggattaacaa aaatgcgcgt gacgtctttg tcctgggcgg
    38761 cgagtttgat caagctgaaa atttctggct tccacagcgt ggagacaacg tgtttaccgt
    38821 cgcgggaaac taagtccagt gcttgcgggc gcaagagctg cgcggaggtc cagcgagttt
    38881 tcggcagttg cagaaagata tcgacatcca gtccggtctg gtggctggca tgaccgccgt
    38941 tgaaacgccc accagcgggc atccccatat cgccaatcag caccgtaccc atgcccagat
    39001 tgctcacctg gctactcaga cgctggataa acatcaccag atccgggtga ccgaaatagc
    39061 gacgctgatc ggtacgcatg acctgataat gttcggactg tatcggcaac gtgtcagcgc
    39121 cgacaataca gccattagaa aaactgccta tcgattgtgc gctacccggc acaggttggg
    39181 ttattttttg ccacggcgtc gctgccaggc tagcactact ggcaagcaga gccagcagcg
    39241 caatcgcggt tttgttcatt ttttaccagc gtggaatatc agtcttcaca tcggcatttt
    39301 gcgcccgctg ccgtaacagg tgatccatta aaacgatcgc cagcatcgct tcagcgatcg
    39361 gcactgcacg gatcccgaca cagggatcgt gacggccttt ggtgatcatc tcaacttctt
    39421 cgccaaagcg gttaatagtg cgccccggca cggtaatgct ggaggtcggt ttcagcgcca
    39481 tatgagcaat gatttgctgc ccgctgctga taccgccgaa aatgccgccc gcatggttgc
    39541 tctggaaacc gtctttggtg atttcgtcgc ggttctggct gccgcgcagc gccactacgt
    39601 caaaaccgtc gccaatttcc acgcctttca ccgcgttgat gctcatcagc gcatgggcga
    39661 tgtcggcatc caggcggtca aagaccggct cgccaagtcc ggcggggaca ccactggcaa
    39721 caacggtgac tttcgcgccg atggagtcgc cctctttttt cagcgcgcgc atcagttcat
    39781 ctaacgcgtc gattttgtcg gggtccgggc agaagaacgg attttgttca accagcgacc
    39841 agtctttgat ttccagcgga atgtcgccca tctgggtcag gcagccgcgg atttcaatac
    39901 caaatttctc ggcgagatat tttttggcaa tcgcccctgc cgccacgcgc atggcggttt
    39961 cgcgggcgga agaacgtcca ccgccgcgat aatcgcgcag accgtatttt tgttcgtagg
    40021 tgtaatcagc atggcctgga cggaagacgt ctttaattgc gctgtaatcc tgagaacgct
    40081 ggtcggtatt ttcgatcaac aagccaatgc tggtgccagt agtaacgcct tcaaaaacac
    40141 cggagagtat tttgacctga tccggctcgc ggcgctgggt ggtatagcgc gatgtcccag
    40201 ggcgacgacg atcaaggtca tgttgcaggt ccgcttccgt cagcggaatg cctggcggaa
    40261 caccatcgac gatgcagccg agcgccagcc cgtgcgattc gccgaaggtg gttacgcgaa
    40321 agagttgtcc aattgtgttt ccagccatca cggctccgtt attgttgtgt ttgcgtgttt
    40381 acttaatctt tataaatcgc gaaatgttct cgtgcggcaa taagctgctc tttagtgagc
    40441 ataaacacac catcgccgcc gttatcaaac tccagccagg tgaacggaac atcgggatat
    40501 tgttccataa gatgtaccat gctgttgccc acttcacaaa tcaacacgcc atcatcagca
    40561 aggtaatctg ccgcgttacc gagaatgcga cgcgtcagtt tcaggccgtc ggtgccagat
    40621 gccaggccca gttccggctc gtgacggtat tcgtttggca ggtcggacat atcttccgcg
    40681 tcgacatacg gcgggttagt gacaatcaga tcgtattgca ctttcggcaa atcacggaac
    40741 agatcggaac gaatcggaat gacgttgtgg atcagaccgt gttcttcgat gttctgttcg
    40801 gcaaccgcca gcgcgtcggg agagatatct accgcgtcga cttccgcttc cgggaaggca
    40861 taagcacagg caatggcgat gcagccgcta ccggtacaca tatctaaaat atgctgcggt
    40921 tgcttgctga tgagtccggc aaatttattg ttgatcagtt caccaatcgg cgagcgcggc
    40981 accagcacgc gttcatcgac gtaaaattca tgaccgcaga accaggcttt gttggtcagg
    41041 taagccaccg gaatgcgctc gttgacgcgg cggatcacgc gttcaacaat acggtgtttt
    41101 tcgctggagg tcagacgcgc ggtgcgcata tcttccggga tatccagcgg caggtagagc
    41161 gatggtaaca ccagctgtac ggcttcatcc cacgggttat cggtgccgtg accgtaccag
    41221 atatttgccg cgctgaagcg gcttaccgac cagcgcaata tgtcctgaat ggtttgcagc
    41281 tcatttactg cttcatcaac gaaaatttta tccacgtatt cctccagggc atgatcgcaa
    41341 taatttcggc ggctagtttg ccatgaagat gacgataaat cagcattcac gcgcggtgag
    41401 tgaggaaaaa tacgtttaaa acgatcgatt gtgctacgag tcgggtaaac tgtaggaaaa
    41461 ttagaaatag agacagataa atgaaaaaga aaacaacact tagcgaggag gaccaggctc
    41521 tgtttcgcca gttgatggcg gggactcgca agattaagca ggacacgatt gtccaccgac
    41581 cgcagcgtaa aaaaatcagc gaagtgccgg tgaaacgctt gatccaggag caggctgatg
    41641 ccagccatta tttctccgat gagtttcagc cgttattaaa taccgaaggt ccggtgaaat
    41701 atgttcgccc ggatgtcagc cattttgagg cgaagaaact gcgccgcggc gattattcac
    41761 cggaattgtt tttggattta cacggtctga cgcagctgca ggccaagcag gaactgggcg
    41821 cgttgattgc cgcctgtcgc cgcgaacatg tgttttgtgc ctgcgtgatg catgggcacg
    41881 ggaagcatat tttgaagcaa caaacaccgc tgtggctggc acaacatccg catgtgatgg
    41941 catttcacca ggcaccgaaa gagtacggcg gtgatgcggc tttgttggtg ttgattgaag
    42001 tggaagagtg gttgccgccg gagttgccct gaatgctggc agcaaatgcc ggagcagcgt
    42061 aaacgcctta tccggcctac aaatatgtga taattcaata aattgcaatt cacttcgtag
    42121 gcctgataag acgcatcaga gcaatactac gaacttcagt tcattcaata catactgaaa
    42181 acgacacgtc gaatcaatcg gttgcccact actggtattc acattcacca caatatcgtg
    42241 tagtacttta tccgttaccg gatgacgagt tggcgtaacc gttaccgttg atttatcaca
    42301 accgtaagag gtaaaggtta acaattctga taccgcgcgg cagtatgctc ctctgccatc
    42361 attatcagaa aaattactgc catcacagag tctgccgcct tccatcaggt aggtcgaaag
    42421 cgtggtggtc actgttgacc ccgtggactg aattcgtaac gccttattat ggctgacata
    42481 attttcactc caggagatat cgtatgaagc gaccggtatc gttgggcata ttgtgttatg
    42541 cactgtaggg gccgtatagc ttccaaatcc gggtaaatct gtataggaat aaaaggatgc
    42601 gatccaaggg caaccataat catcagatgc aacggtaatt ttaaacgtca tatatgcaca
    42661 acgaaaaatt ttcgtatcta tgccagggca atttgagtta ttaattgtat caaagtcagt
    42721 cagtaccaaa gttggagctg aacccgaccc agaccaggaa ataacccgca acgtatcgcc
    42781 tttatacgca aaagaatcat atttcccata tgcaacctcc tttggcgtta tcaccgtaac
    42841 attgaaaaca ctgctcgcta cagcaccggt taccttgtcc atggcataat aaactctcac
    42901 actgtcaaaa ctcgcactca taagtttatc tccagcataa ctcttagatg cgccgacaac
    42961 actcagaacg agcagcatta ggctcgcgaa aattatggtc cattttttca tttcagaaat
    43021 actcaaaggt tgccgtcact gtggcactaa agtctccgga cgttacatct cgcccgctct
    43081 ttgcctgtaa ccaggtatta aaatttaggc tgttatttcc ctgattcagc gggagcgtga
    43141 cgcctgttgt attgttgatt ggcaccgatg tgcctgccgc agtttcaata ccaatcccag
    43201 cgccggatgc tgatgacgag gagtcaaagg ccagaaagcc gggtagagca ctatcttctg
    43261 ttcctgtcaa cgttactttg acattaaaca atgtagaact atgacaatct ttcaattgaa
    43321 acaccaatgg cactcgctct gactgcccaa ggttcattaa atcactggcg gcaatggcag
    43381 gaaaatgtac ttctgccaac tggctaccat taatcactgg cgtacacgat ttactgatta
    43441 aattgccggt aaagtgcaaa ttatctcctg ctattgtggt tccactgccc agcaacatca
    43501 ggctactaat aaaaaacaac gtacgctttt tcttcattgg taatctacct gtaacgtggc
    43561 ccaggcctca aagtccgcct ctggcagaat gacaccactc ttttttactg gcacagccgt
    43621 taacaccggt aaatcggttt cattcaccac aagtggcgta ttgatcgtaa aactctgccc
    43681 tgcctgtttg agctgaatgc ctaaaccagt gatattgctg cttactgcag cattatcaaa
    43741 atcgcttggt gtgccactcc agctcaaggt catttccatt aatgggtcgc gaaccgccgt
    43801 atcgcaggta atggaatacg gcacggtttg ctcataattg ctgccattaa tgtcatcaat
    43861 aagcacatcc tgaaaagtta tttcaatggt ttgagcgttg ctaatcgtgc agttaggcgg
    43921 ctgcactaaa taaccgtgaa aagagacgtt tgagagtgcc atcgagcaaa agccccacag
    43981 caaaataagc gagatccgtt tcattggtaa tccaccacaa ttgtggcact ggcgttaaat
    44041 tcagctgccg ctaacgttgt accactctgt ttcaccggca cagcttccag aacgggaact
    44101 cccgaggaaa aattaaatgg caaccatgag ccagaggtta agtcgagtat tgtgtgatca
    44161 ctacttttct gaacccgaat accaaaccca ggaatacccg tgctgataac tgtttcaccg
    44221 ttaatcacga ccgttgtcgc ctgcatctgt agacgcaggt catccattgc cagattgtta
    44281 caaacaagcg agtagtcaat gggacgttta taatcaaccc tattgatctt attaatgaat
    44341 acattaccaa actcaactgc cgcaccattt accgtacaag gaggaggggc atcgacaagc
    44401 acccgtaaat tcatacttac tgacttactc gctgcaactg caaagccgct accaacacaa
    44461 catatcagta acccagttac cgcccagaca gaacaagatc ctttcatact ggctctcccg
    44521 gcctcagcct ttgcggctct gctcttcatc gacctagcaa ctgttaccgt tgcaagtgaa
    44581 aaagagcggc atccgagcgc catagtcgtt gacataggtc agtaccggca cgctacccat
    44641 cttcacattc agcggtgcag aggttttcgg cgacatcact attggcgaga aaccggctgc
    44701 cggattgcca tttttctgtg tgctggcatt actgaaaatg acgtaatacg acgtcgggtt
    44761 attgacggta aattcctgac cgttgcgggt taaagtaatc ttatattgct aggggttttt
    44821 catgctgacg ttctccagcg ccttcggtcg ccagaacaac ttaatacgcg tttgtagtgc
    44881 tatttgcagc gtgttggctt tgtttgattt tggcgggatc tcacgcacgt tgaagtagaa
    44941 caggctttca cgatcggcag gcaatttgtt gatatccggc attccctgca cttttacctg
    45001 cccgttcatc attgaatcga tgcgttgcac cggtggcaga acagttaacg gtgaactgat
    45061 cttgttaccc ttttcatctt cgatccagct ttgggccaga taaggcagtt tcggatcgtt
    45121 attgcgcaac gtcacgctga ttgatttgtc gctttcatta aatatcaaac gggtacgatc
    45181 tggcgttaca gaagcctggg cagacaaact ggcagcgcta agcagtaacg ctagcgtcgt
    45241 agcacccaat ttggcagagc aaagtaaatc agacatggat cattccttaa tcacgttatt
    45301 ggttagctga aacagaaaca gggggattgg tgggtgtcac ctgttgcgtc tgctcctgaa
    45361 tgacaggctt tatgtcatca ggaacaaccg gcgcgacgtt gcctttatgc tggcatggca
    45421 gtaacaagcc gttaaacaga tcggcaggca gcgggtccgg caggttgata tcgcaatgcg
    45481 caacaccact ccagaacaca ctcatgtgtt cgccaggttt cactccagcc agataaacat
    45541 tgccgtcatc atcgacaagg cccactgtct gctcgttatc attttttact tctgcgccaa
    45601 acggaggatg gctgccgtct tgtaaacgca gcaccgccat cgctttttga ccactgatga
    45661 cggcaaattt gcggtagcca atcgcccctt cagttagcgt ggcttgtacc accgactggg
    45721 ttgcttcggc gttttccggc agtttgttga ggtcgatata cgcctgattg cggtaatagt
    45781 tattgacgtc agaaacgacg gctttaccaa acatattggt ataaacagca gcaccgttac
    45841 cttctaccgg aacatcggca acgccatcgg catcaatcaa caagcgtgtc ccgcccatat
    45901 tctgggtacg gtgaagtgcg ccaccgtggg cagtaagcgt tgcgccgccc tgtaacgaca
    45961 agcccgcaga ggtgtattgc ccttcatggt agttcgcact gaggtcaacc tgcgccagcg
    46021 aaccatcatg gctgtaatag ccgtcaacgc tggtgtgttt gtcactggtg ccgacgttca
    46081 actgatagtg agtcgcgtca tcgacacggc tgaaataacc gacctgactg ctgtccgtcc
    46141 cactgccata gtaccgttat agctaacggt actgttgtcg ccccacggca tactgagcga
    46201 aatgtacatg cctttgtcgg cctggttgtc atactcgtaa cggtagccag tcatcgagat
    46261 gctgacatta cgaatactgc ccatattgaa atagtgcgag agcatgatgt tgtagtttgt
    46321 ctgctcctcg cgatcccagt aggtatggcg ggtatagttg agataaaccg aaacacccgc
    46381 atcgcggaag ttctggttat aagtggcggt gtacatctct ttgtcgttgc ccgtgcgtac
    46441 cattccgctg tcactggcat ccagatactc gctcatggtc ataaagttct cttccgagaa
    46501 gcgatatcca gcaaaagtaa cgcggctgtt gagctggtca aagtctttgg aataactcac
    46561 acggaaggag ttaccgtcca gcgaaccttt gccataagcg gtgtctttat ccagtttggt
    46621 gtgcgagtga gtaacatcaa acgcgaccgc gccgaatgta gacaaatcgc gaccgacacc
    46681 aagcgccgca gactgatagt tttcatctcc cagtgcgccg ccatataacg accagccgtt
    46741 agcgatcccc caggaagctt cagcaccaga aaaaaattca ccctcgacat ggtgtcccca
    46801 ctcttgcgga cggcccatca tgatcttata gcgaacctga cctggacgag tgaggtatgg
    46861 catcgaggcg gtgctgatgt catattcctg cacctggccg ttctgttctt caatgcgaat
    46921 atgcaacgta ccggagacag aatcaccaag atcctgaata cgaaacggtc cagccggaac
    46981 ctgcgtttcg taaatcacac gccccatctg gctgacggtc acttttgctg tggtgtgtgc
    47041 cacgccggaa atgtctggcg cgtagccgcg cagattggga ggcaacattt gatcgtcagt
    47101 actgacactg ccaccaacat agttaaaacc atcaaaaata tcggatctga ggtaatcctc
    47161 gcccagcgcc agtttggctt ttaatgatgg taacgcccgc ctggcatagt agcgactcca
    47221 ctcccatttt tttgagtttc atcgccgctg aattcatcgt catcattact gcgagtatgt
    47281 tgatagttag tctgccagtc agcacgcata cgccacggcc ctaggttaac cccaaccgtc
    47341 ccgttgccgc tgatctcgtt actatcatca ccgccatttt cttcgtgccg ggtttgtgcg
    47401 ttgatgctgt agtccgcgac gatcccggag atgccgtcat cccaacgtga aggcggatcc
    47461 ctgtcgggat aagtatattc gaggtaagcc tgtggcagtg aaatgactaa tgcggactgg
    47521 cttaaatcag ccttaatttc catgccttcc agttgaccgg atttcaggca tttagcatcg
    47581 tggctccatt gcagattttt cgccacgtct tcttttaaac caaactgcgc taccagttcc
    47641 ggtgtcagac aagcatagct tttgctcgcg tcatcttcac cagcatacca gtaaatatcg
    47701 tactcttccg ccaatggctg tttatttagt tgaacctgta aattgtattt tccgggctca
    47761 acatatccct ggctggaaaa acgcttcata tcaatttttg tgtcgccttt taattccaga
    47821 aaacgggaat caaactgaat gtcgtcttca gcccagacac tgctataact ccctgacatt
    47881 gccagcgcaa tgcaccacgg aagaatccgt aatcgaaaaa gagaatggtc aggcatacac
    47941 gtttctatcc gtttttattc ttgttcggtc aaaatacatt taaatccccc atagcagtgt
    48001 cgctatgggg gtatgcctta caggtaagta atctggaagg tggtatttgc ttcaaaatta
    48061 cccagatctg gcgcatcagc tgcgccgacc agccaggcgt taaagttcag tgtctgcttc
    48121 gctttgcctt tgttggtaga agtgtctttc actattttct ggtcaatacc catgccgctt
    48181 ttgtaagagg tacccagaga gtcaccgatc gccaggctaa cattgttaaa tgccgcaccg
    48241 gtatcggtat tgaaaatggt gtaataattg ccgctatttg cagaagaaac ggtaccagta
    48301 aaggtagtgg tcattgtttc ctgagtatca aatacgcaat cttgcagacg aatctggaaa
    48361 cttttcggcg tggtggtgcc gttatttttc agtttttcgg caccgatatc acccatatca
    48421 acttccagtt tgtgatcgtc cggaacgata gaacacactg agtcttcaat ggtgccgtaa
    48481 aaacgtgcag taccgttgtt acccgcagcg attgttgccg tagaggtgaa cacgcccatc
    48541 accattgccg cagcaatagc tgttttaaca aacttactca ttatttgtcc ttaaactata
    48601 aata
//
LOCUS       DAZSNO010000015        46569 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZSNO010000015 DAZSNO010000000
VERSION     DAZSNO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46569
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.015"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            113..667
                     /gene="pinE"
                     /locus_tag="ACHWN2_000802"
                     /pseudo
     CDS             113..667
                     /gene="pinE"
                     /locus_tag="ACHWN2_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(1006..1854)
                     /locus_tag="ACHWN2_000803"
     CDS             complement(1006..1854)
                     /locus_tag="ACHWN2_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR8930448.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            2063..2698
                     /gene="yfhb"
                     /locus_tag="ACHWN2_000804"
     CDS             2063..2698
                     /gene="yfhb"
                     /locus_tag="ACHWN2_000804"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR8930449.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2756..3259
                     /gene="tadA"
                     /locus_tag="ACHWN2_000805"
     CDS             2756..3259
                     /gene="tadA"
                     /locus_tag="ACHWN2_000805"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR8930450.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(3256..4812)
                     /gene="mltF"
                     /locus_tag="ACHWN2_000806"
     CDS             complement(3256..4812)
                     /gene="mltF"
                     /locus_tag="ACHWN2_000806"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR8930451.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            5071..8958
                     /gene="purL"
                     /locus_tag="ACHWN2_000807"
                     /gene_synonym="purI"
     CDS             5071..8958
                     /gene="purL"
                     /locus_tag="ACHWN2_000807"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR8930452.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            9516..10943
                     /gene="qseE"
                     /locus_tag="ACHWN2_000808"
     CDS             9516..10943
                     /gene="qseE"
                     /locus_tag="ACHWN2_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR8930453.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            11108..11821
                     /gene="qseG"
                     /locus_tag="ACHWN2_000809"
     CDS             11108..11821
                     /gene="qseG"
                     /locus_tag="ACHWN2_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR8930454.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            11811..13145
                     /gene="glrR"
                     /locus_tag="ACHWN2_000810"
     CDS             11811..13145
                     /gene="glrR"
                     /locus_tag="ACHWN2_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR8930455.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            13206..13544
                     /gene="glnB"
                     /locus_tag="ACHWN2_000811"
     CDS             13206..13544
                     /gene="glnB"
                     /locus_tag="ACHWN2_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR8930456.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(13589..14779)
                     /gene="hmpA"
                     /locus_tag="ACHWN2_000812"
     CDS             complement(13589..14779)
                     /gene="hmpA"
                     /locus_tag="ACHWN2_000812"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR8930457.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            15106..16359
                     /gene="glyA"
                     /locus_tag="ACHWN2_000813"
     CDS             15106..16359
                     /gene="glyA"
                     /locus_tag="ACHWN2_000813"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR8930458.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(16557..17750)
                     /locus_tag="ACHWN2_000814"
     CDS             complement(16557..17750)
                     /locus_tag="ACHWN2_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFR8930459.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            17868..21149
                     /locus_tag="ACHWN2_000815"
                     /pseudo
     CDS             17868..21149
                     /locus_tag="ACHWN2_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            21246..22228
                     /locus_tag="ACHWN2_000816"
                     /pseudo
     CDS             21246..22228
                     /locus_tag="ACHWN2_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            22251..23762
                     /locus_tag="ACHWN2_000817"
     CDS             22251..23762
                     /locus_tag="ACHWN2_000817"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR8930460.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            23787..24785
                     /locus_tag="ACHWN2_000818"
     CDS             23787..24785
                     /locus_tag="ACHWN2_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8930461.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            24818..25912
                     /locus_tag="ACHWN2_000819"
     CDS             24818..25912
                     /locus_tag="ACHWN2_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR8930462.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            25924..26796
                     /locus_tag="ACHWN2_000820"
     CDS             25924..26796
                     /locus_tag="ACHWN2_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR8930463.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(26844..27266)
                     /locus_tag="ACHWN2_000821"
     CDS             complement(26844..27266)
                     /locus_tag="ACHWN2_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR8930464.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(27363..28570)
                     /gene="hcaD"
                     /locus_tag="ACHWN2_000822"
                     /pseudo
     CDS             complement(27363..28570)
                     /gene="hcaD"
                     /locus_tag="ACHWN2_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(28580..29392)
                     /gene="hcaB"
                     /locus_tag="ACHWN2_000823"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(28580..29392)
                     /gene="hcaB"
                     /locus_tag="ACHWN2_000823"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR8930465.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(29389..29709)
                     /locus_tag="ACHWN2_000824"
     CDS             complement(29389..29709)
                     /locus_tag="ACHWN2_000824"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR8930466.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(29709..30227)
                     /gene="hcaF"
                     /locus_tag="ACHWN2_000825"
     CDS             complement(29709..30227)
                     /gene="hcaF"
                     /locus_tag="ACHWN2_000825"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR8930467.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(30224..31585)
                     /gene="hcaE"
                     /locus_tag="ACHWN2_000826"
     CDS             complement(30224..31585)
                     /gene="hcaE"
                     /locus_tag="ACHWN2_000826"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR8930468.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            31721..32611
                     /gene="hcaR"
                     /locus_tag="ACHWN2_000827"
     CDS             31721..32611
                     /gene="hcaR"
                     /locus_tag="ACHWN2_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR8930469.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            32771..33910
                     /locus_tag="ACHWN2_000828"
     CDS             32771..33910
                     /locus_tag="ACHWN2_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR8930470.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(33902..35182)
                     /gene="csiE"
                     /locus_tag="ACHWN2_000829"
     CDS             complement(33902..35182)
                     /gene="csiE"
                     /locus_tag="ACHWN2_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR8930471.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(35373..36227)
                     /locus_tag="ACHWN2_000830"
     CDS             complement(35373..36227)
                     /locus_tag="ACHWN2_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR8930472.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(36372..37175)
                     /gene="suhB"
                     /locus_tag="ACHWN2_000831"
     CDS             complement(36372..37175)
                     /gene="suhB"
                     /locus_tag="ACHWN2_000831"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR8930473.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            37294..38034
                     /gene="trmJ"
                     /locus_tag="ACHWN2_000832"
     CDS             37294..38034
                     /gene="trmJ"
                     /locus_tag="ACHWN2_000832"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR8930474.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            38304..38792
                     /gene="iscR"
                     /locus_tag="ACHWN2_000833"
     CDS             38304..38792
                     /gene="iscR"
                     /locus_tag="ACHWN2_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR8930475.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            38904..40118
                     /gene="iscS"
                     /locus_tag="ACHWN2_000834"
     CDS             38904..40118
                     /gene="iscS"
                     /locus_tag="ACHWN2_000834"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR8930476.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            40146..40532
                     /gene="iscU"
                     /locus_tag="ACHWN2_000835"
     CDS             40146..40532
                     /gene="iscU"
                     /locus_tag="ACHWN2_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR8930477.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            40549..40872
                     /gene="iscA"
                     /locus_tag="ACHWN2_000836"
     CDS             40549..40872
                     /gene="iscA"
                     /locus_tag="ACHWN2_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR8930478.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            40968..41483
                     /gene="hscB"
                     /locus_tag="ACHWN2_000837"
     CDS             40968..41483
                     /gene="hscB"
                     /locus_tag="ACHWN2_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR8930479.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            41500..43350
                     /gene="hscA"
                     /locus_tag="ACHWN2_000838"
     CDS             41500..43350
                     /gene="hscA"
                     /locus_tag="ACHWN2_000838"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR8930480.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            43352..43687
                     /gene="fdx"
                     /locus_tag="ACHWN2_000839"
     CDS             43352..43687
                     /gene="fdx"
                     /locus_tag="ACHWN2_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR8930481.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            43699..43899
                     /gene="iscX"
                     /locus_tag="ACHWN2_000840"
     CDS             43699..43899
                     /gene="iscX"
                     /locus_tag="ACHWN2_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR8930482.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            43958..45241
                     /gene="pepB"
                     /locus_tag="ACHWN2_000841"
     CDS             43958..45241
                     /gene="pepB"
                     /locus_tag="ACHWN2_000841"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR8930483.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            45383..46159
                     /gene="sseB"
                     /locus_tag="ACHWN2_000842"
     CDS             45383..46159
                     /gene="sseB"
                     /locus_tag="ACHWN2_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR8930484.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(46191..46316)
                     /gene="timP"
                     /locus_tag="ACHWN2_000843"
     CDS             complement(46191..46316)
                     /gene="timP"
                     /locus_tag="ACHWN2_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR8930485.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
BASE COUNT        10985 a        11707 c        12796 g        11081 t
ORIGIN      
        1 taaaaaaaca gtacattcta ttttataatg actgtgtatt cccatggaag gagatcaccc
       61 aattctcccc cccgactttt gacgcagtgt cttgttaaca gggggattga ccatgcttat
      121 tggctatgta cgcgtatcaa caaatgacca gaataccgat ctacaacgta atgcgttgaa
      181 ctgtgcagga tgcgagctga tttttgaaga caagataagt ggtacaaagt ccgaaagacc
      241 ggggctgaaa aaactgctca agacattatc ggcaggcgac acactggttg tctggaagct
      301 ggatcggctg gggcgcagta tgcggcatct tgtcgtgctg gtggaggcgt tgcgcgaacg
      361 aggcatcaac tttcgtagtc tgactgattc aattgatacc agcacaccaa tgggacgctt
      421 tttcttccat gtgatgggtg ccctggctga aatggaacgt gaactgattg ttgaacgaac
      481 aaaagcagga ctggaagctg ctcgcgcaca gggacgaatt ggcggtcgcc gtcccaaact
      541 ttcaccagaa taatgggcgc aggccgggcg actaattgca tcaggagttt ctcgccagaa
      601 ggtggcgatc atctatgatg ttggcgaatc gactttgtat aagaggtttc cagccagggt
      661 tatataaaac cgcagacacg gcgtatacaa gaacgtgcta cggttgactg aaaagttaat
      721 gttgtatgtc acaggccttt tgcgccgtaa agccccgtcc tttagggcgc ggaggacgtt
      781 aaagaaagtg gtgttctttg tgttgctgaa cttatgcaaa atttttttgc accataaatc
      841 caacaacaca ttcaataaca cattgctgta tcactatgaa aataaatgtg tttttatttg
      901 atttcacaca tatcacaatt gatgcatttt ttagtgatta acagcgccat aaggaaactc
      961 tcaaaacaac acaaaccggg cgggcattat acgcgcattc tttactcaga ccagtttttt
     1021 caccagcgct tcactatgac gaatacgttc tggtgccagt tccagatcct gctgaatcag
     1081 cgcaatgaac agcaaatccg ttaacattcc ctgagcgtga caagcagaga ttgaagcact
     1141 gtttgtcgct tgttcttcgg caatggtata caggcaatga gaagcacgct gttgcagggc
     1201 attcggagta aagccggtaa tcgccagcac ttttccgccc actcgcagca tctcatctgc
     1261 cgccaggttt aactcgcgtc gtacaccggt gtaggaaatg gctaataaca gatcgtcagg
     1321 ggacgacgcc tgtactgttg cgagtagcgc atgcatatcg cgcagtgcgg cagcattgaa
     1381 gccaatcttc atcagcttcc aggcaaagtt ttgcgccacc agacccgaag cgccaatacc
     1441 ggtcagaatt atccgccgcg cagagcgcaa cattgttacg cattcatgca gtttctcttc
     1501 actattaacg tttagcgttg cgtacatagc ggcggtattt tctttaatca gtttttcgcc
     1561 gaccagccgt aacggatcat caccgcggat ttggttatga atgggcacgg agggcgattc
     1621 cggctggctt gccagcgctt cactcaacgc caacttaagc gccggaaaac ctttatagcc
     1681 gagtttttgc gcgaacttca cgacgctgga ctgactgact ccggcttcgt tggccagttg
     1741 ctgagagctt aaatggcgcg ccgtatcagg ttgtagcagc agataatccg ccagtttttt
     1801 atcactctgg gcaagccctt ggtaacgctg acggatacga agtaagccgt tcatatcttt
     1861 cctcgcgatg aaatagtggc gacaacaggc aatatgagac acacgcgagt gaattttata
     1921 ttccattgcc gctaattgcc atgaattaaa aattccagtt agccatgatg cggtcgattg
     1981 ttgccggaaa agtgatgatc tatctgttag gctatgtagt agcgaaaatt aatcaggatg
     2041 tttcagtcca gaggagtatg gtttggcaac tcacgagcgt cgtgtggtgt tttttgactt
     2101 agatggaaca ttgcatcagc aggatatgtt cggcagtttt ctgcgctatt tactgcgtcg
     2161 ccaaccgctg aatgcgttac ttgtcctgcc gttgttaccg attatagcca ttgcgttatt
     2221 gataaaaggt cgtgcggcac gctggccgat gagtctgctt ctgtgggggt gcacttttgg
     2281 tcacagcgaa gcacgtttac agacgttgca ggccgatttc gtgcgctggt ttcgcgacaa
     2341 tgttaccgcc tttccgctgg ttcaggagcg attaaccacc tacctgttaa gttccgatgc
     2401 tgatatctgg ttgattaccg gctctccgca gccgctggtt gaagcggttt atttcgatac
     2461 gccctggctg ccgcgggtta atcttatcgc cagccaaatt cagcgtggct atggtggttg
     2521 ggtattgacg atgcgttgtc tgggacatga aaaggtcgca caactggagc gcaaaatcgg
     2581 cacgccgctg cggctgtaca gtggctatag cgacagtaat caggacaatc cgctgcttta
     2641 tttctgtcag catcgttggc gagtaacccc gcgcggtgaa ctccagcaac tggaatagag
     2701 taaagcatag cgtccgtgta taatgcgccg cgcttttata accggagttt tctttttgtc
     2761 tgaagtcgaa tttagccacg aatattggat gcgtcacgcg ctgacgctgg cgaaacgtgc
     2821 ctgggatgag cgggaagtgc cggtcggcgc ggtattagtg cataacaatc gggtaatcgg
     2881 cgaaggctgg aaccgcccga ttggtcgcca tgatcccacc gcacatgcag aaatcatggc
     2941 cctgcggcag ggtggtctgg tgatgcaaaa ttatcgtctg atcgacgcca cgttgtatgt
     3001 cacgcttgaa ccatgtgtaa tgtgtgccgg agcgatgatc cacagtcgca ttggtcgcgt
     3061 ggtctttggt gcgcgtgacg cgaaaactgg cgctgcggga tctttaatgg atgtgctgca
     3121 tcatccgggt atgaatcacc gagtggaaat tacggaagga atactggcgg atgagtgcgc
     3181 ggcgttgctc agtgacttct ttcgcatgcg ccgccaggaa attaaagcgc agaaaaaagc
     3241 gcaatcctcg acggattaat tttgtttctc ttcactccct ttcctggaaa acagcagaga
     3301 gggagaatgg gtcaaatagt ttgatgacga ctgggaaaga aacgagagaa aaggaaattt
     3361 ctctttgccc aactccgtag gcgataccgc cggataatcc tgcgccagtt gcatcgccgc
     3421 ttctgtagcc tgcttctctt tctcttgcag ataacccacc aggctaatct gatacttacg
     3481 aatattttcg acataagcgt aggcttcatg cccacgagcg tagccgtaag tcagcttgct
     3541 gtaatagggt ttctggctaa gtaaaggcag acgctgtttt acgtcagccc aactgtcagg
     3601 attccctttg gtttttgccg tcagggcgcg ggcatccagc atatgcgcat agcccatatt
     3661 gtacgcagcg agggcaaacc agatccgttc gttctccggc acactttccg gcactttact
     3721 catcatatcc tgcaaataac gcacgccacc gctgatgctc tgttcggcat cggtacgatc
     3781 cgtaatgccg aggctttgcg cggtattttt ggttaacatc atcatgccgc gcacacccgt
     3841 cggtgaagtg gcctgtgcat cccagtgcga ttcctgataa gcaatagcgg ccagcaaacg
     3901 ccagtcaatt tcttcggcgt atttctcaaa caggggcttt aactgcggca gtaccgcatc
     3961 gacggcgcgt aaaaatgtgc gcgtatcgac gtaatcaaaa tcatcgccat gccccaggta
     4021 tttctcttca atgcgtgcca gcgtaccgtc ttcattcatt tcgttgaaga agtcgagcag
     4081 ggcggcggaa agggtattat cgccatctaa cgggctaaac caagtcaccg gttgttcatc
     4141 ggtgatatcg agcgctacgg cgagctccgg gtgaacgcgc tgaaacaggc tgatggcgac
     4201 agaatcagca atggtgtaat cgagttttcc ttcgatgaca tcttccatta attccgcaga
     4261 gccttttttg tcgtctacct tccagcttaa ttccgggaat tttgtttctt tcagggtctg
     4321 gagatcgtta actaccacat gacccggtgc aacggtgagt tgctccgccg tcaggttgcc
     4381 cagcgtacgt gggcgatact gacccacttt ataaaccagt tgttgtgaca cggaataata
     4441 ggtagggcca ggctgataat tttttacccg ctcactgtta tagaccagtc ctgccgccag
     4501 caggtcggcg ttaccattat caaggtcgtc aaacagctgg ctgatattct gccgcacggt
     4561 cactttcagt tttacgccga ggtaatcggc aaactgtttc gccagttcgt aatccaggcc
     4621 aaaaggtttc ccgttgattt cgttataagt caggggagta tgaatggtgc tcacacgcaa
     4681 ctctccccgc gcttgaatgg cggcgatacg gttgtcggct ttaccaaacc agggaatgga
     4741 tggccagaga gcgaccgcga gcagcagtgc cagaatgccg atgaacagat aattaatctt
     4801 taattttttc aattagttaa ttctctgtgt cgtgcgcgtc ccagcttgaa aaaacgtaat
     4861 aatagtgaaa ggtttactca taaatgagcg gcattttgcg taaacctgcg ccagatggca
     4921 acttattaca gccattggcg gcacgcgttg ctaattcacg atggtgattt tatttccacg
     4981 caaacggttt cgtcagcgca tcagattctt tataatgacg cccgtttccc cccccttggg
     5041 tacaccgaaa gcttagaaga cgagagactt atgatggaaa ttctgcgtgg ttcgcctgca
     5101 ctgtcggcat tccgaatcaa caaactgctg gcacgttttc aggctgccag gctcccggtt
     5161 cacaatattt acgccgagta tgtccatttt gctgacctca atgcgccgtt aaacgatgat
     5221 gagcacgcac aacttgaacg cctgctgaaa tatggtccgg cactcgccag ccacgccccg
     5281 caaggcaaac tcctgctggt gaccccgcgt cctggcacca tctctccctg gtcttcgaaa
     5341 gcgaccgata ttgcccataa ctgcgggcta caacaggtaa accgccttga gcgcggcgtt
     5401 gcttactata tagaagccgg tacgctgacc aatgaacaat ggcagcaggt taccgctgaa
     5461 ctgcacgacc gcatgatgga aacggtcttt tttgatttag atgatgcaga gcagttgttt
     5521 gcccaccatc aaccgactcc ggtcacaagc gttgatttgc tggggcaggg ccgtcaggcg
     5581 ctgatcgacg ctaacctgcg tcttggcttg gctctggcgg aagatgaaat tgactatctg
     5641 caggatgctt tcacaaagct tggtcgtaac ccgaacgaca tcgagctgta tatgttcgct
     5701 caggcgaact ctgagcactg ccgccacaaa atttttaacg ccgactggat tatcgacggt
     5761 gaacaacagc cgaaatcgct gttcaggatg atcaaaaata ctttcgaaac cacgccagat
     5821 cacgttctct ctgcttataa agataacgcc gccgtaatgg aaggttctga agtgggccgc
     5881 tactttgctg accacgaaac gggccgctac aatttccatc aggaaccggc gcacatcctg
     5941 atgaaagtcg aaactcacaa ccacccgacg gcgatttctc cgtggccggg cgcggcgacc
     6001 ggttccggcg gtgaaatccg cgatgaaggt gccaccgggc gcggcgcgaa gccgaaagct
     6061 ggtttggttg gtttctccgt atccaacctg cgaattcctg gcttcgaaca gccgtgggaa
     6121 gaagatttcg gtaagcctga gcgcattgtt accgcgctgg acatcatgac cgaaggcccg
     6181 ctgggcggtg cggcgtttaa caacgaattt ggtcgtccgg cactgaacgg ctacttccgt
     6241 acttatgaag aaaaagtgaa cagccacaat ggcgaagagc tgcgcggtta tcacaaaccg
     6301 atcatgctgg cgggcgggat cggcaacatt cgcgccgatc acgtacaaaa aggcgagatc
     6361 aacgtcggtg cgaagctggt cgttctcggc ggcccggcaa tgaacatcgg tcttggcggc
     6421 ggcgcggcgt cttccatggc gtctggtcag tctgatgccg acctcgactt tgcttccgta
     6481 cagcgcgaca acccggagat ggagcgtcgt tgccaggaag tgatcgaccg ttgctggcag
     6541 cttggtgatg ctaacccaat tctgtttatc cacgacgtcg gtgcgggcgg tctttctaac
     6601 gcgatgccgg agctggtgag cgacggcgga cgcggcggta aatttgaact gcgcgatatt
     6661 ctaagcgacg aaccgggcat gagcccgctg gaaatctggt gtaacgaatc ccaggaacgc
     6721 tacgtgctgg cggttgctgc cgatcaatta ccgctgtttg acgaactgtg taagcgtgag
     6781 cgcgcaccct acgcggtgat tggtgaagcg accgaagaac tgcatctttc tctgcacgat
     6841 cgtcattttg ataatcagcc gatcgatctg ccgctggacg tcctgcttgg taaaacgccg
     6901 aagatgaccc gcgatgtaca aacgctgaaa gcgaagggcg atgcgctggt ccgtgaaggg
     6961 atcaccattg cagacgcggt gaaacgcgta ctgcatctgc cgaccgtggc ggagaaaact
     7021 ttcctggtga ccatcggtga ccgcagcgta accggcatgg tggcgcgcga tcagatggtg
     7081 gggccgtggc aggtgccggt cgctaactgc gcggtcacta ccgccagcct cgacagctac
     7141 tacggtgaag cgatggcgat tggcgagcgt gcgccggttg cgctgctgga tttcgccgcc
     7201 tctgcccgtc tggcggtcgg tgaagcgtta accaatattg ccgcaacaca gattggcgat
     7261 atcaaacgca ttaaactttc cgccaactgg atggcggcgg caggccaccc tggtgaagat
     7321 gcgggcctgt atgaagccgt taaagccgtg ggtgaagaac tttgtccggc gctgggcctg
     7381 acgatcccgg tgggtaaaga ctctatgtcg atgaaaaccc gctggcagga aggtaacgaa
     7441 gagcgcgaaa tgacgtcgcc gctgtcgctg gtgatttctg catttgcccg cgtggaagat
     7501 gtacgtcaca ccatcacgcc gcagctttgt accgaagata acgcactgct gctgattgat
     7561 ttgggcaaag gcaataacgc gctgggcgca acggcgctgg cgcaggttta tcgtcagctt
     7621 ggcgacaaac cggcagatgt acgcgatgtt gtccaactga aaggcttcta tgacgcgatt
     7681 caggcgctgg ttgcacagcg taagctgctg gcgtatcacg accgctctga tggaggcctg
     7741 ctggtaacgc tggcggaaat ggcctttgca ggtcattgtg gcattgacgc ggatatcgcc
     7801 tctctgggtg acgatcgcct ggcggcgttg tttaacgaag aactgggtgc ggtgattcag
     7861 gttcgtgccg ctgaccgtga agcggtcgag tccgtactgg cacagcatgg gcttgcggat
     7921 tgcgtccatt atgttgggca ggcggtttcc ggtgaccgtt ttgtgattac cgccaacggg
     7981 cagactgtat tcagcgaaag ccgcactacg ttgcgtgtct ggtgggcaga aactacctgg
     8041 cagatgcagc gcctgcgtga caacccggag tgtgccgatc aggagcatca ggcgaaatct
     8101 aacgacgccg atccgggcct gaatgtaaaa ctgtcgttcg atatcaacga agatgtggca
     8161 gcaccgtata ttgccactgg cgcacgtccg aaagttgccg tactgcgtga gcagggcgtg
     8221 aactcgcatg ttgaaatggc ggcagctttc caacgtgggg gctttgatgc tatcgacgtg
     8281 catatgagcg acctgctgac tggacgtacg ggactggagg attttcacgc cctggtcgcg
     8341 tgcggaggtt tctcctacgg tgatgtgctg ggtgccggtg aaggttgggc gaagtctatc
     8401 ctgttcaatg accgtgtacg cgatgagttt gcaaccttct tccatcgtcc gcaaacgctg
     8461 gccctggggg tatgtaacgg ttgccagatg atgtctaacc tgcgtgaact gatcccgggg
     8521 agtgagttgt ggccacgttt tgtgcgtaac acctccgatc gctttgaagc gcgtttcagc
     8581 ctggttgaag taacccaaag cccgtctctg ctgttgcagg ggatggtggg ctcgcaaatg
     8641 ccgattgctg tctctcatgg tgaagggcgc gtggaagtgc gtgatgcggc gcatctggcg
     8701 gcactggaaa gcaaagggct ggtggcactg cgctatgtcg ataacttcgg caaagtcact
     8761 gaaacctacc cggctaaccc gaacggttca ccgaacggta ttacggcggt tacgactgaa
     8821 agtggtcgag tcaccattat gatgccgcac ccggaacgtg ttttccgtac tgtcagcaac
     8881 tcctggcatc cggaaaactg gggcgaggat ggcccatgga tgcgcatttt ccgcaatgcg
     8941 cgtaagcagt tggggtaagt catcagttca ttggttttgc agcccggatg cggcattaag
     9001 caccaaccac tatcataagc tcccaactcc gggagctttt ttgtgtctgt aaatcacgac
     9061 aatgggtggt ttaccgtgtc gctttctggc gacacttaaa tgattgattt tattgtaatc
     9121 taataagttt atcttaagtt gtctcttttt agcgacacag tggctgattt acattcaaat
     9181 agcgaagaag ccataaatcc ctcaaagcaa cgtataatca gtacgttacc aaactatttt
     9241 ctttattggc acagttactg cataatagta agcagtggct cattcaccga cttatgtcag
     9301 ccccttcggg acgtgctaca taaaattcga atgacgcaca acaaggtgcc tgccgtccaa
     9361 cttctgatat cagcgtagct atatcaacca tcgggcgaaa cgtcgagtta ggcaccgcct
     9421 tattccataa caaagccggg taattcccgg ctttgttgta tctgaacttc ccctcggtta
     9481 gcatcaggct attcgcgtct gacgagagta acaccttgaa acgctggccc gtttttcccc
     9541 gctcattacg acaactggta atgctggcat ttttgctaat tctactaccc ctgttggtgc
     9601 tggcatggca agcctggcaa agcctgaatg cgcttagcga tcaggcggcg ctggttaacc
     9661 gcactacgct tattgatgcc cggcgtagtg aagcgatgac caacgcggcg ctggagatgg
     9721 agcgtagcta ccgtcagtat tgcgtgctgg acgaccctac gcttgcgaag gtttatcaaa
     9781 gccagcgcaa gcgttacagc gaaatgctcg atgcccacgc aggcgtactg ccagacgata
     9841 aactttacca ggcattgcgt caggatttga acaatctggc ccaacttcag tgtaacaaca
     9901 gtggtcccga tgctgccgcc gccgcgcgtc ttgaagcctt tgccagtgcc aataccgaaa
     9961 tggtgcaggc cacgcgcaca gtggtgttct ctcgtgggca gcaacttcag cgtgaaatcg
    10021 ccgaacgtgg gcaatatttt ggttggcaat cgctggtgct atttctggtg agtctggtga
    10081 tggtgctact tttcacgcgg atgattatcg ggccggtgaa aaatatcgag cgcatgatca
    10141 accgtctggg ggaagggcgt tctctgggca atagcgtttc gttctgtgga ccgagcgagt
    10201 tacgctcggt tgggcaacgt attctttggt taagtgagcg cctgtcatgg ctggaatccc
    10261 aacgccatca atttttaaga catttatctc atgaattaaa aacgccactg gcgagtatgc
    10321 gcgagggcac tgaattactg gctgatcagg ttgtcgggcc gcttacgcca gagcaaaaag
    10381 aggtggtgag cattcttgat agcagcagct gcaatttgca aaaactgatc gaacaactgc
    10441 ttgattacaa ccgtaaacag gcggacagtg cggtggaact ggagaatgtt gaattagcac
    10501 cgctggtgga gacagtggtt tctgctcata gcctgcccgc acgggctaaa atgatgcata
    10561 ccgacgtcga tctcaaagca acagcttgcc tggcggagcc aatgctgctg atgagcgtac
    10621 tggataatct ttactccaat gcggtgcact acggggctga atccggtaac atttgccttc
    10681 gcagcagttt acatggtgcg cgggtttata ttgatgtcat caatacaggc acgcccattc
    10741 cgcaagagga acgcgccatg atcttcgaac ccttttttca gggaagccac cagcgaaaag
    10801 gggcggtgaa gggcagcggt ctgggattaa gcattgccag ggattgtatt cgccgtatgc
    10861 aaggggaact gtatctggtc gacgagagcg ggcaagacgt ttgtttccgc attgaattac
    10921 cgtcgtcgaa aaacacgaaa taatgctaac agcctatcct atcaggctat tttatttgtc
    10981 agttcggccc cgggtagtgc tcgaaatctt cacgtacgat atgtacgccc cggtttctcc
    11041 gcgctgtcca tgtccagact gtctgcaaca attacgccca ctgggatagg ctttaagtct
    11101 ggtgaatatg cgacacattt ttcaacgatt attgccccga cggctgtggc tggcagggct
    11161 gccatgtctg gcattgctgg gttgcgtgca gaatcacaat aagccagcca ttgatacgcc
    11221 agcagaagaa aaaattccgg tttatcaact ggctgactat ctttctactg aatgtagcga
    11281 catctgggcg ctgcaaggta aatcaaccga aaccaatccg ctttactggc tgcgggcgat
    11341 ggattgtgct gatcgtttaa tgcctgcgca gtcgcgtcag caagctcgcc aatatgacga
    11401 cggcagctgg caaaatacct ttaaacaagg gattttgctc gccgacgcca aaattacgcc
    11461 gtatgagcgc cgccagctgg tcgcgcgtat tgaagcgtta agtaccgaga tcccggcaca
    11521 ggttcgccca ctgtatcaac tctggcgcga cggtcaggcg ctgcaactgc aactggcgga
    11581 agagcgccaa cgttacagta aactccagca gtccagcgac agcgagctgg atacattgcg
    11641 ccagcaacat cacgttctac agcagcaact ggaactcacc acccgcaagc tggaaaacct
    11701 gaccgatatt gaacgccagc tctcgacccg caaaccggct ggaaatttct cgccggatac
    11761 gccgcacgag agtgaaaagc ccgcgccatc cacccatgag gtcactcctg atgagccata
    11821 aacctgcgca tttattattg gtcgatgacg atccgggatt gctgaaactg cttggcctgc
    11881 gcctgaccag cgaaggctac agtgtggtca cggcggaaag tggcgctgaa ggattacggg
    11941 tactgaatcg cgaaaaagta gatttagtta tcagcgacct gcggatggat gaaatggacg
    12001 gtatgcagct atttgctgaa atccagaaag tgcagccggg aatgccagta attattctta
    12061 ctgcgcatgg ttctatcccc gatgccgttg ctgcaacaca gcagggcgtt tttagtttcc
    12121 tcaccaagcc tgtcgataaa gatgcgctat atcaggcaat tgacgatgcg ctggagcaat
    12181 ccgcgccagc caccgatgaa cgctggcgcg aggcaattgt cacccgcagc ccgctgatgc
    12241 tgcgtttgct ggaacaggcg cggctggtgg cgcaatcaga cgtcagcgtt ttgattaacg
    12301 gtcagagcgg caccgggaaa gagattttcg cccaggctat ccacaacgcc agcccgcgca
    12361 acagcaaacc atttattgct attaactgtg gcgcattgcc cgagcaattg ctggagtcgg
    12421 agctgtttgg tcatgcgcgt ggcgcgttta ctggcgctgt cagcaatcgc gaaggtttat
    12481 tccaggcggc ggaaggcgga acgctatttc tcgatgaaat tggcgatatg cctgcgccgt
    12541 tacaggtcaa actgctgcgc gtgttgcagg agcgtaaagt gcgcccgctg ggcagtaacc
    12601 gcgatattga tatcaatgtg cggattattt ctgccactca ccgtgatctg tcaaaagcga
    12661 tggcgcgcgg ggaattccgc gaagatctct attaccgcct caacgttgtc agcctgaaaa
    12721 ttccggcact ggcggagcgc acagaagaca ttccgctact ggcaaatcac ctgttgcgcc
    12781 aggcggcaga gcgacataaa ccgtttgtcc gcgcgttctc taccgatgcg atgaaacgcc
    12841 tgatgaccgc gagctggccg ggtaatgtgc gccagttggt caacgtgatt gaacagtgcg
    12901 tggcgctgac ctcatctccg gtgattagtg atgcgctggt ggagcaggcg ctggagggtg
    12961 aaaatacggc gctgccaacc tttgttgagg cacgtaatca ttttgaactc aactacttgc
    13021 gtaagctgct gcaaatcacc aaaggcaacg tcacccacgc ggcgagaatg gcggggcgca
    13081 accggacaga attttataaa ctgctttccc gacacgagct ggatgcaaac gatttcaagg
    13141 aatgaattgg cgttatgtgt tacgtttagc agatcaaaag acaggcaacc ttttcaagga
    13201 atagcatgaa aaagattgat gcgattataa aacccttcaa gctggacgat gtccgcgaag
    13261 cactggccga agtcggtatt accggcatga cggtgaccga agtgaaaggc tttggtcgcc
    13321 agaaaggcca taccgagctg taccgcggcg cggagtatat ggtggatttt ctgccgaaag
    13381 tgaaaattga gattgtcgta ccggacgaca ttgtcgatac ctgtgtcgat accattattc
    13441 gcacggcgca aaccggcaaa atcggtgacg gtaaaatctt cgtctttgac gtggctcggg
    13501 tcattcgcat ccgtaccggt gaagaggacg acgcggcaat ttaaaccgcg tcaagggttg
    13561 ccggatgttt ccatccggca acatcaaatt acagcacctt atgcgggcca aagcattcgt
    13621 aatgaatgtt ttcctgcttc acgcccagat ccactaactg tttcgcggta aactgcatga
    13681 agccaaccgg gccgcagaga tagaactgca ttgtcggatc gctgaacgca ccttccagtt
    13741 tgctcaaatc catcagacct tcgctatcaa actgaccttt agcgcgatcg gcttcgctcg
    13801 gctgacgata ccaggtgtgc gcggtaaagc gcggcagtga ctgccccagt tccttaactt
    13861 catcagcaaa ggcgtgaact tcgccatttt ctgccgcatg gaaccagttc acttgtgctg
    13921 tgtggcctgc ttttgccagc gtgtcgagca ttgccagcat tggcgtttga ccaacaccgg
    13981 tagagattaa cgtcactggt gtgtcatctg cgacagccat aaagaaatca cctgccggag
    14041 cgaccagttt cacgacatcg ccaacattgg cgtgattgtg caaccagttg gatacctgcc
    14101 caccctcttc gcgtttcacc gcgatacgat agcctttgcc atccggtttg cgagtcaaag
    14161 agtactgacg aatttcctga tgcgggaaac cttccggctt cagccagacg ccgagatatt
    14221 gccccggacg gtattctgcc actgcgccac cgtcgaccgg ctccagttcg aagctggtga
    14281 taagcgcgct gcgcggtgtt ttagccacaa tgcggaaatc gcgagtacct tcccaaccac
    14341 cggctttgct ggcgttttcg ttatagattt ccgcctcgcg attgataaat acattagcca
    14401 gtacaccata ggctttaccc cacgcgtcca gcacttcctg ccccgggctg aacatttcgt
    14461 ccagcgttgc caacaggtgt tcaccgacga tgttgtactg ttccggtttg atctggaagc
    14521 tggtgtgctt ctgcgcgatt ttttctaccg ctggcagcag cgcaggcagg ttttcaatat
    14581 tactggcgta ggcggcaata gcgttaaaca gggcttcacg ttgatcgcca ttacgctggt
    14641 tactcatgtt aaaaatttct ttgagttctg ggttatgagt aaacatacgg tcgtagaaat
    14701 gggcggttaa ctttggcccc gtttccacca gtaaagggat ggtggctttt actgtagcga
    14761 tggtttgagc gtcaagcata tggtcttcct tttttgcatc ttaattgatg tatctcaaat
    14821 gcatcttata aaaaatagcc ctgcaatgta aatggttctt tggtgttttt cagaaagaat
    14881 gtgatgaagt gaaaaatttg catcacaaac ctgaaaagaa atccgtttcc ggttgcaagc
    14941 tctttattct ccaaagcctt gcgtagcctg aaggtaatcg tttgcgtaaa ttcctttgtc
    15001 aagacctgtt atcgcacaat gattcggtta tactgttcgc cgttgtccaa caggaccgcc
    15061 tataaaggcc aaaaatttta ttgttagctg agtcaggaga tgcggatgtt aaagcgtgaa
    15121 atgaacattg ccgattatga tgccgaactg tggcaggcta tggagcagga aaaagtacgt
    15181 caggaagagc acatcgaact gatcgcctcc gaaaactaca ccagcccgcg cgtaatgcag
    15241 gcgcagggtt ctcagctgac caacaaatat gctgaaggtt atccgggcaa acgctactac
    15301 ggcggttgcg agtatgttga tatcgttgaa caactggcga tcgatcgtgc gaaagaactg
    15361 ttcggcgctg actacgctaa cgtccagccg cactccggct cccaggctaa ctttgcggtc
    15421 tacaccgcgc tgctggaacc aggtgatacc gttctgggta tgaacctggc gcatggcggt
    15481 cacctgactc acggttctcc ggttaacttc tccggtaaac tgtacaacat cgttccttac
    15541 ggtatcgatg ctaccggtca tatcgattac gccgatctgg aaaaacaagc caaagaacac
    15601 aagccgaaaa tgattatcgg tggtttctct gcatattccg gcgtggttga ctgggcgaaa
    15661 atgcgtgaaa tcgctgacag catcggtgct tatctgttcg ttgatatggc gcacgttgcg
    15721 ggcctggttg ctgctggcgt ctacccgaac ccggttcctc atgctcacgt tgttactacc
    15781 accactcaca aaaccctggc gggtccgcgc ggcggcctga tcttggcgaa aggtggtagc
    15841 gaagagctgt acaaaaaact gaactctgcc gttttccctg gtggtcaggg cggtccgttg
    15901 atgcacgtaa tcgccggtaa agcggttgct ctgaaagaag cgatggagcc tgagttcaaa
    15961 acttaccagc agcaggtcgc gaaaaacgct aaagcgatgg tagaagtgtt cctcgagcgc
    16021 ggctacaaag tggtttccgg cggcactgat aaccacctgt tcctggttga tctggttgat
    16081 aaaaacctga ccggtaaaga agcagacgcc gctctgggcc gtgctaacat caccgtcaac
    16141 aaaaacagcg taccgaacga tccgaagagc ccgtttgtga cctccggtat tcgtgtaggt
    16201 actccggcga ttacccgtcg cggctttaaa gaagccgaag cgaaagaact ggctggctgg
    16261 atgtgtgacg tgctggacag catcaatgat gaagccgtta tcgagcgcat caaaggtaaa
    16321 gttctcgaca tctgcgcacg ttacccggtt tacgcataag cgaaacggtg atttgctgac
    16381 aatgtgctcg ttgttcatgt tggatgcggc atgaacacct tctcaggcct acaaaatctt
    16441 gcaaattcaa tatattgcaa tctccgtgta ggcctgataa gcgtagcgca tcaggcaatt
    16501 tttcgtttat gatcatcaag gcttccttcg ggaagccttt ctacgttatc gcgccatcaa
    16561 atctgtcgta actgcgcctc aacatacaaa tagccaattc ccagcacctg ttgtgcgcgg
    16621 cttaattgcc cgaagccaat ttgcgtcgct ttcacgctcg gtccttcgtc gcggtcgaac
    16681 gggttaaatc ctgtctggcg aataatagta ttaagccagt tctcaccaaa ggcacaactg
    16741 cgaccgtaca accaaatctg attaatattg aggatgttga ggaagttata cagactcaaa
    16801 ccaatggcat tggcagagcg atcaacccag ctggtgatcc acggttctcc actctgccag
    16861 gcagcgatta actgcgcggt tgtcagtttt tcaggctcaa gctgagtatt aaccggttgt
    16921 gattttagcc atacccgcgc ctgttttttt aatgcactta acgaggcgac ggtttccagg
    16981 ctgccgtaac gtccacagtc gcagacgact ccatccggat taacgatggt gtgaccaatc
    17041 tgtccgctac catacaaact gccgcgataa atttgctcgt taatcacgaa cgacgagcca
    17101 atgccgtaat caacgttgat cacgcagaaa tcccgttcct gcgaattatt ttgccatttc
    17161 tccgccagcg ccagcatcac gcagtcatta tcgaccagca cccgaatgcc cagcttctct
    17221 tccagcagat acttcacctc aactggcgtt gtccacggcg cttgcggcat ggtttgcgat
    17281 acgccggtca ccggatcaac ctgcccgtgg attgccagcg ccagattgat ggtgcggtcc
    17341 ggccacaatt tacggtggcg atgccagcat ttttcgattt cggacagcag cgcctgcggc
    17401 gtcggtacat caatctgtaa atactcaaat tcgcctttcg gacttaaaca agcattagca
    17461 acctgacact caatactggt gggcgtcacg ttcaggcaca gcgtccagtc accttccggc
    17521 gcaatcagcc atgtaccgct actatgcccg cgcgtctggc tttcatcgtc aatattcacc
    17581 acccgttttt cgctttccag ttcctgcaaa atattactga ctgccggaat cgaaatttgc
    17641 gccagccggg ccagcgttga tttattggcg cgcttttgcc ggtacagcag ttccagaatc
    17701 acgcatttgt tatggtggcg aatctgttga ttgttaatgc aggctctcat cttcactaaa
    17761 ccttgtttag ttaattgcgt gattattccg cgaaaacatt gccagattgt tcaatacact
    17821 gccacaaatc ttttaattca gtatgtcttg ttaatattga gggcaccatg actccagtaa
    17881 aagtgtggca agagcgcgtt gagatcccga cctatgaaac cgggccgcag gatatacatc
    17941 ccatgttcct ggaaaatcgc gtttatcagg gatcgtccgg cgcggtttat ccctacggcg
    18001 tgaccgatac gctgagcgag cagaaaaccc tgaaatccta gcaggcggtg tggctggaaa
    18061 acgactacat caaagtgatg atcctgccgg aactgggcgg tcgggtgcat cgcgcatggg
    18121 ataaagtgaa acagcgcgat tttgtctatc acaatgaagt cattaaacct gcgctggtgg
    18181 ggatgctggg accgtggatc tccggcggga ttgagtttaa ctggccgcaa caccatcgcc
    18241 cgaccacctt tatgcccgtt gatttcaccc tcgaagccca tgaagacggt gcacagacgg
    18301 tgtgggtcgg cgaaacggag ccgatgcatg gtttacaggt gatgacaggt ttcaccctgc
    18361 gccctgaccg ggcggcactg gaaatcgcca gccgcgtcta taacggcaac gccacgccgc
    18421 gtcatttctt gtggtgggtc aacccggcag tgaaaggggg tgaagggcat cagagcgttt
    18481 tcccaccgga tgtgactgcg gtgtttgatc acggcaaacg ggccgtctcc gctttcccca
    18541 tcgccaccgg cacttactac aaagtggact actccgccgg agtggacatt tctcgctata
    18601 aaaatgtgcc tgttccaacc tcatatatgg ccgaaaaatc gcagtacgat tttgtcggcg
    18661 cgtggtgtca cgatgaagat ggcggtttgc tgcacgttgc caaccaccat attgcgccag
    18721 gtaaaaaaca gtggagttgg ggacacagtg aatttggcca ggcgtgggat aagagtctga
    18781 ccgacaataa cggcccgtat atcgaactga tgaccggtat ttttgccgat aaccagcctg
    18841 attttacctg gcttgatgct tacgaagaga agcgtttcga gcagtatttc ctgccttatc
    18901 attctctggg catggtgcaa aatgcctccc gcgatgcggt gataaaactc cagcgtagtg
    18961 agcgggggat tgagtggggg ctgtatgcca tctctccgtt gaacggatac cgtctggcga
    19021 tccgcgaaat cggcaaatgc aacgcgttgc tcgatgatgc cgtggccctg acaccagcga
    19081 ccgccatcca gggcgtgttg cacggtatca atcctgacag actgaccatt gaactctccg
    19141 atgccgacgg caatattgta ctgagttatc aggaacatca gccgcaggag ttgccgttgc
    19201 cggacgtcgc caaagcgcca ctggcagcac aagacattac cagtacagat gaagcctggt
    19261 ttatcggtca gcatctggag caatatcatc acgccagccg ttcaccgttc gattactacc
    19321 tgcgcggcgt ggcgctggac ccgctggatt atcgctgtaa cttggcgctg gcgatgctgg
    19381 aatataaccg cgcagatttc ccgcaagcgg tggcgtatgc cagtcaggcg ctgaaacgtg
    19441 cacatgcgct gaacaaaaaa ccgcagtgtg gacaagcaag tttgattcgc gccagtgctt
    19501 acgaacgtca gggacaatat caacaagccg aagaggattt ctggcgtgcg gtctggagcg
    19561 gcaatagtaa agcaggcggc tattatggtc tggcacgact ggcggcgcgt aatgataact
    19621 tcgacgcggg tctggatttt tgccaacaaa gtcttcgtgc ctgcccaacc aatcaggaag
    19681 tgctttgctt gcataatctg ctgctggtgt taagtggtcg tcaggacaac gcgcgtttgc
    19741 agcgcgagaa actgctgcgt gattatccgc tgaacgccac tctgtggtgg ctgaactggt
    19801 tcgatggtcg tagcgaatca gccctcgcgc agtggcgcgg tctgtgtcag ggacgcgacg
    19861 ttaacgctct gatgaccgcc gggcaactga ttaactgggg aatgcccacc ctggcggcag
    19921 agatgctgaa cgcactggac tgccagcgca cgctgccgct ttacctgcaa gccagcttgc
    19981 tgccgaaagc cgaacgtggc gaactggtcg caaaagccat tgatgtcttc ccgcagtttg
    20041 tccgtttccc gaatacgctg gaagaagtgg cggcgctgga gagtattgaa gagtgttggt
    20101 ttgctcgcca tttactggcc tgcttctact acaacaaacg tagctacaac aaagccattg
    20161 ccttatggca acgttgcgta gagatgtcgc cggagtttgc cgacggctgg cgcgggttag
    20221 cgatccatgc gtggaataag aaacacgatt atgagctggc cgcgcgttat cttgataatg
    20281 cttatcagct tgcgccgcag gatgcacgtc tgcttttcga acgggatttg ctggataagt
    20341 taagtggagc cacaccggag aaacgactgg cgcgtctgga aaataatctg gaaattgcgc
    20401 tgaaacgcga cgacatgacc gcagaactgc tcaatttgtg gcatctcacg ggtcaggcag
    20461 acaaagcggc ggacattctc gccacgcgta aattccaccc gtgggaaggc ggggaaggga
    20521 aggtcaccag tcagtttatc ctcaaccagt tattacgcgc ctggcagcat cttgatgcca
    20581 gagagccgca gcaggccagc gaactgcttc atgccgcgct gtattatccg gagaatttaa
    20641 gcgaaggccg tttaccgggg caaactgata acgacatctg gttctggcag gcgatatgcg
    20701 ccaacgcgca gggcgatgaa actgaagcga tgcgttgttt acgtctggcg gcgaccggcg
    20761 atcgcaccat taacatccac agttattaca acgatcagcc ggttgattat ctcttctggc
    20821 aaggaatggc gctgcgactg ctgggtgaac agcaaaccgc acagcaactg tttagtgaaa
    20881 tgaaacagtg ggcgcaagag atggcgaaaa ccagtattga ggcggatttc tttgctgttt
    20941 cacaacctga cctgttatcg ctgtatggcg atttacaaca gcagcataaa gaaaaatgcc
    21001 tgatggtggc gatgctggcg tccgcgggac tcggggaggt tgcgcaatat gagtctgctc
    21061 gcgctgaatt gacggcgatt aatccggcct ggccgaaagc ggcattattc accactgtga
    21121 tgccttttat ttttaactac gttcactaaa ccctattaac tgtaatattt gagcggcacg
    21181 cgctgccgct catcacaaaa taattagaat aagccgttgt taataaaaac cctacaggag
    21241 gcactatgcc taaaaaaatg agaacaaccc gtaatctatt attaatggct acgctattag
    21301 gtagcgcact atttgccagg gctgcggata aagaaatgac cattggtgca atataccttg
    21361 atacccaggg atattacgct ggagtgcgtc agggcgttca ggatgcggca aaagattctt
    21421 cagtacaggt acagttaatt gaaactaacg cccaggggga tatttcgaaa gaaagtacct
    21481 ttgttgatac cctcgtggcg cgtaatgtcg atgccattat tttatcggca gtgtctgaaa
    21541 atggcagtag ccgtaccgtt cgtcgcgcca gtgaagcggg tattccggtg atttgctaca
    21601 acacctgtat taatcaaaag ggtgtcgata aatatgtctc ggcgtatctg gtcggcgatc
    21661 cactggaatt tggtaaaaac tgggtaacgc tgccgccgat tattttattg ccaataaaat
    21721 tgaccagccg aaaattgccg tcatcaattg cgaagccttt gaagtttgtg tgcagcgacg
    21781 taaaggattt gaagaagtat taaaatcccg cgttcccggc gcgcaaattg tcgctaacca
    21841 ggaagggact gttttagata aagcgatttc cgttggtgaa aaactgatta tctccacgcc
    21901 ggatctcaac gccattatgg gggagtcggg cggtgcgaca ctcggcgcgg taaaagcggt
    21961 acgtaatcaa aatcaggccg gaaaaattgc tgttttcggt tcggatatga caaccgaaat
    22021 tgctcaggag ctggaaaaca atcaggtgct gaaagcggta gtggatattt ccggtaagaa
    22081 aatgggcaat gctgttttcg cgcaaacatt gaaggttatc aataaacaag ccgacggtga
    22141 aaaagtgatt caggtgccaa tcgatctcta taccaaaacg gaagatggta aacagtggct
    22201 ggcaacgcac gttgatggtc tgccctaatc gctgattcgc agaggtccct atgttcacgg
    22261 caacagaggc agtcccggta gcaaaagtgg tggcaggaaa taagcgttac cccggcgtcg
    22321 ttgcgctgga taacgttaac ttcacgctca ataaaggcga agttcgtgcg ctgttaggta
    22381 aaaacggcgc gggcaaatcg actctcattc gaatgcttac cggcagcgaa cgcccggata
    22441 gcggtgatat ctggattggc gagacgcgac tggaaggtga cgaagctacg ttgactcgcc
    22501 gtgccgctga actgggggtt cgtgcggttt atcaggaatt aagtctggtg gaagggctga
    22561 cggtggcaga aaacctctgc cttggtcaat ggcctcgccg caacggcatg attgattacc
    22621 tgcaaatggc gcaggatgcc caacgttgct tacaggcgct gggcgttgac gttagtcctg
    22681 aacaacttgt ttcaacgcta agcccggcgc aaaagcaact ggtggaaatt gcacgggtga
    22741 tgaagggcga gccgcgcgtg gtcattcttg atgaacccac cagctcgctt gccagtgcag
    22801 aagttgaact ggtgatcagt gcggtgaaaa agatgtcggc actgggcgtg gcggtgattt
    22861 atgtcagcca ccggatggaa gaaatccgcc gcattgcctc ctatgccacc gttatgcgcg
    22921 atggtcaggt ggcgggcgat gtgatgctcg aaaacacctc cacgcatcat attgtgtcgc
    22981 taatgctagg gcgtgatcac gttgatattg cgccggttgc ccctcaggaa attgtggatc
    23041 aggccgtact ggaagtccgt gcgttacgcc ataagcccaa gctggaggat atcagcttta
    23101 cgctacgtcg tggcgaagtg cttggcattg ctggcctgct gggggcaggg cgcagtgaat
    23161 tgctgaaggc gattgttggg ctggagacgt atgaacaggg cgaaattgtt atcaacggcg
    23221 agaaaatcat gcgccccgat tacggcgaca tgctgaaacg cggcattggc tatacgccag
    23281 aaaaccgcaa agaagcgggg atcattccct ggctaggcgt tgacgaaaat acagtgctga
    23341 ccaatcggca aaaaatcagc accaacggtg tgctgcaatg gtccaccatc cgccgcctga
    23401 ccgaagaggt gatgcagcgg atgacggtca aggctgccag tagcgaaaca cccatcggca
    23461 cgctttctgg tggcaatcag caaaaagtgg tgatcggtcg ttgggtctat gccgccagcc
    23521 agattttgtt gctcgacgag ccaacgcgcg gcgtcgatat cgaagccaaa cagcagattt
    23581 accgtattgt ccgtgagctg gctgccgaag gaaaaagcgt ggtgtttatc tccagtgaag
    23641 tggaggaatt gccgctggtg tgtgaccgca tcctgttatt acagcacggc acgttctcgc
    23701 aggagtttca ctcgccggtc aatgtggatg agctgatgtc cgccattctg tctgtgcact
    23761 gatgaatttt accgaggaac ctgaagatgt atgcttcgtc attaccattg ccgcagggca
    23821 agagcgtctc gctcaaacaa tttgtcagtc gccatattaa tgagatcggt ttgctggtgg
    23881 tgattgccat tctttatctg gtcttctccc tgaacgcgcc tggctttatc tcattgaata
    23941 accagatgaa cgtgctgcgc gatgccgcca ccattgggat tgccgcctgg gcgatgacgc
    24001 tgattattat ctccggtgaa attgatgtca gcgttgggcc gatggtggct tttgtctcgg
    24061 tgtgcctggc atttttgctg caatttgaag ttccgttggc gattgcttgt ctgctggtgt
    24121 tgctgttagg cgcgctgatg ggaacgctcg ccggggtgct gcgcggcgtg tttaacgtac
    24181 caagtttcgt tgccacactg gggctgtgga gcgctctgcg cggaatgggg ctgtttatga
    24241 ccaacgcact gccagtgccg attgacgaaa acgaggtgct ggactggctg ggcggacaat
    24301 ttctcggtgt gccagtatcc gcgctgatca tgatggtgtt gtttgcgctg tttgtgttca
    24361 ttagccgaaa aaccgccttc gggcgctcgg tttttgccgt tggcggtaat gccacggcgg
    24421 cgcagttgtg cggtattaac gttcgtcgag tgcgcattct tatctttacc ctttcgggat
    24481 tattagcggc ggtgaccggc attttgttgg cggcacgcct cggttccggt aacgcaggtg
    24541 ccgcaaacgg tctggagttt gacgtcatcg ccgcggtcgt ggtcggcggt acggcacttt
    24601 ccggcggtcg cggttccctg ttcggtacat tgcttggtgt gctggtgatt acgctaatcg
    24661 gtaacggtct ggtgctgctc ggtattaact cctttttcca gcaggtggta cgcggcgtca
    24721 tcatcgtggt ggcggtgctg gcgaatatct tgctgaccca gcgaagcagt aaagcgaaac
    24781 gctaaacctt aacgcccctg gcgcgggtgc tgcagggatg aaaattaatc gtattgttaa
    24841 tgagggattt atgaaaacga tgctggctgc ttatttacta ggaaattcaa ccgtcgatct
    24901 gcgggaagtt gcggtgccga cgccggggat taaccaggta ctgatcaaaa tgaaatcctc
    24961 cgggatttgc ggaagcgatg tccactatat ctatcatcaa caccgtgcca cggcggcggc
    25021 acccgataaa ccgttatacc agggatttat caacggtcat gaaccgtgcg ggcagattgt
    25081 ggcgatgggg caaggctgcc gccattttaa agagggcgac cgcgtgctgg tgtatcacat
    25141 ttctggctgt ggtttttgcc cgaactgccg ccgcggcttt cctatctctt gtactggcaa
    25201 aggaaaagcg gcttacggct ggcagcgtga cggcggtcat gccgaatatc tgctggcgga
    25261 agaaaaagat ctgatcctcc tgccggatgc gctgagctac gaagatggtg cgtttatcag
    25321 ttgcggcgtt ggcacggcct atgaagggat tttgcgcggc gaagtttccg gcagcgataa
    25381 cgtgctggtg gtcggtctgg ggccggtcgg catgatggcg atgatgctgg cgaaaggtcg
    25441 cggtgcaaaa cggatcatcg gcgttgatat gctgccggaa cgtctggcga tggcaaaaca
    25501 gttagggggg atggatcacg gctatttagc aaccaccgaa ggtctgccgc agataattgc
    25561 tgaacttacc cacggtggcg cggatgttgc gctcgattgt tccggtaatg ccgcaggtcg
    25621 cttgctggca ctgcaatcca ctgctgactg ggggcgggtg gtttacattg gtgaaaccgg
    25681 aaaagtggaa ttcgaggtca gcgctgatct gatgcaccat caacggcgga ttatcggctc
    25741 ctgggtgacc agtctgttcc atatggaaaa atgcgcccac gatttgactg actggaagct
    25801 gtggccgcgt aacgccatta cccatcgctt ctcgctagaa caggcaggtg atgcctatgt
    25861 gctgatggcg agcggcaaat gcgggaaagt tgtgattaac ttcccggatt aaggagttag
    25921 cggatgacca tctatacctt atcccacggg tcgctaaagc tggacgtttc tgaccagggc
    25981 ggcgtaatcg aaggcttctg gcgcgatacg acgccgctct tacgtcctgg taaaaaaagc
    26041 ggtgtggcaa ccgatgcctc atgttttccg ctggtgccat ttgcaaaccg ggtgagcggc
    26101 aaccgttttg tctggcaggg gcgtgagtat caactgcaac cgaatgttga gtgggatgcg
    26161 cactatctac acggcgatgg ctggctgggc gagtggcaat gtgtctcgca cagcgatgat
    26221 agtttgtgtc tggtgtatga acatcgcagc ggtgtctatc actatcgggt aagtcaggcg
    26281 tttcatttaa cggcggatac gctgacggtg acgctctctg tcaccaatca aggggcagag
    26341 acgctgccat ttggtaccgg ctggcatcct tattttccgt tgtcaccaca aacgcggatt
    26401 caggcgcagg cgagcggtta ctggctggaa cgggagcagt ggctggcggg tgagttttgc
    26461 gagcagctac cgcaggaact ggattttaac cagctcgcgc cgttgccgcg tcagtgggtg
    26521 aacaatggtt ttgccggatg gaatggtcag gcccgcatcg agcagccgca ggaaggttat
    26581 gccatcatca tggaaacgac gccacctgca ccgtgttatt tcatctttgt ttccgaccct
    26641 gcgtttgata aaggatatgc gtttgatttc ttctgtctgg aaccgatgag ccatgcgccg
    26701 gatgagcatc atcgcccgga agggggtgat ctcattgcgc tagcaccagg ggaatcaaca
    26761 acctcagaga tgtcgttgcg ggtagagtgg ctgtaattta taaaatgccg gataaggcgt
    26821 tcacgccgca tccggcatct ttcctaacgc cgatcgagag aaattgcccc cggtccggta
    26881 attgccaata gcaagaacgc gccagcgata ctgacattct tccagaaatt aatcatattt
    26941 ggcccaaccg catcgccggt catatcccag taatggtgac caatcaccgc cgtacccagc
    27001 gtgtagaaaa taaacagcac cgccagcgga cgggtgaaaa agccaagcac gattaatatc
    27061 gcggcgggca cttccataac taccgcaata atcgctgcca gcatcggcat tggcgcgccc
    27121 aacgaggcca tatattggac cgtaccgtca aagcccatca ttttgggaaa accaaaaata
    27181 atgaaaatta agaccacggc gatacgggca attaataaca aaacggggcg ggcagctcca
    27241 aaatcaaaat aacgtagtgt gttcataacc aatccctttg caagataatg tgatttaaac
    27301 gtaatacaca ctcttgcacc tcgccacgcc ccaggcattg agatattcgc taattatcct
    27361 gattacagtg atttaagcgc gatgttctca tctatcagca gtttcgcatc aaacgttttg
    27421 ccgctctgga tccatttgcg aattgggcga atctcacggc cctgattcag cgtgaccgca
    27481 ccgataagca cgccgttttg cagattaaac caaatcgcct tctgagtttc cgggttgcca
    27541 cgacaaagcc agtcatcgcc acgcatatcg ccaataaact gtaagttatc actgtactga
    27601 tcgctccaga accacggcgg cggcagtagc ggtagcggta gccctaacat tgcggcagcg
    27661 gcaatttgcg cctggttatt ggcgttttcc cagctttcgc agcggtgtag tgcaccatta
    27721 tcaagacgag tgattgccac atcgccaccg gcaaagatcg cgggatcgca ggtgcggcaa
    27781 gcctcatcaa tgacaatgcc attggcagta tcaaggttgg cctcgcgagc cagttgctcg
    27841 ttggcgctga taccaatacc gtaaatcacc acatcagcct gaagcgtttc cccactttgc
    27901 agcgtcagtt ctactttttc accatcgacc acatgttcaa tggcattatt gagcagaatg
    27961 cgcacaccag cctgctgatg gcgctgtaaa agatagcgtt gcacgggcgg tggtgcatta
    28021 cggcccatga cggttgccgc cagttcaatc actgtcacct tacatctgcg ctgcgctgcg
    28081 tggcgctggc agccagttcc agaccaatag ttccggcacc gacaatcacg actgaccgtt
    28141 cgggctgcag aacttctcgc agtctggcgg catcaccggc atggtgtagg gtaaagcagc
    28201 gttctcccag tgcatcaagc aacggcagcg gtcgagctgc cgcgccggtt gctataaaaa
    28261 gctgatccca gtgccagctt tcgccgttgg ttaacactaa ctctcgtgtg tcgcgaccca
    28321 gcgttttgat ggttacaccg gaatgcagat gaacattgtt ttcctgccac cagttagcgg
    28381 gtaacacctg ctgtaactgc ggggaatctt ccagcaacat ggatttcgag agtggaggtc
    28441 gttcataagg aagatgtcgc tcatcggaaa acagatgcag ctcaccggtg aacccttgct
    28501 ggcgtagcga ggccgcagcc attgccgccg cttgcccgcc accgacaata atgatcgttt
    28561 tttctttcat cgtgcttcct taaagatcca gcccagccgc tacgtggcga atgccgcgaa
    28621 tcgctaaacc cgcatcagcg ttgatcatca caccgcttaa tgcgcgatta ttgcgccgcg
    28681 atgccaacat cacatacggc cccgtaaaat ccgccggttg cgggaaaaat tgcagcggta
    28741 aaatggcggc aattttctcc ggcgtcagag actgcattat cgaggtttca ctttgcccga
    28801 gcgcctgtgg gccgcgcagg tcgctggcca taccacacgg gccgacgcca ttcacccgca
    28861 ctttcggtgc cagttcataa gccagttggc gaataagtcc ggttgcggca tgtttactgg
    28921 tggtgtacag cgggccaccg ccgccaggat accaggcggc atttgacagt gtgaaaatca
    28981 tgctgccttc actggcgatt aacgccggag cgcaggcttt tgcgcccagc aggtaaccga
    29041 gtacgttgac gttaaacagc tcgtggaagc cggtttcgag cgtctctgcg ggagtattaa
    29101 ccagtgaggc attgtgatcc cagatgcctg cattgccgat aaaacaatcc agcttgccgg
    29161 aacgagtcag gatctgatcg accgcgcgtt gataatcggc ataacaggtc acgttacctt
    29221 ccaccgccag aatatgttcg ccaaatcgct gacgcagact ggcgactttt gccgccgaca
    29281 gttccagcgt ggcaacttgc gcgccttctt cgataaatcg ctcgaccagc gccagcccta
    29341 atcccgatcc gccgccggta ataaaaatgg actcgttatg cagatcgctc atggctgcgc
    29401 ctccggtaag tcgatgaaaa tgtcaccacc ttcaacgtgt actggataag tggtgagcgg
    29461 atcggtggcg ggcaggcata acgctttccc cgttttcagg caaaaactgg cggcgtgtag
    29521 cgggcactcc accgtggcgt catcttccag atacccttct gacatcgacg cattaccatg
    29581 gctgcaacga tcgttaatgg cataaaactc gccgcccacg ttgaacaagg cgatgacggg
    29641 cgaggtatct atccggagag cctcaccctc cggcacatcc gcaacgggac acgcataaat
    29701 tcgattcatc agaacagtac actcaggtta tgggaagtga tcaccgcttg atccaggacg
    29761 atatcccgtt ccagcaagcg ccagttgtca tcttccagac ggcgaacttt gtcgaaacgc
    29821 gttccaacat agaatgtttc atcgcgctct ttttgtgccc gataaagtag ataattcacc
    29881 ctcacggcaa atacgtttgg gatgtcggtt tcgcttacct ggcagttgct gattaagtga
    29941 cgagtgcgtg acggcggctc ttctgcccag gccatgcccg tttccagacg ggcgattcgc
    30001 cgctccagct ggtctttggt gtcattgaaa atccaggtcg tcggcggctg gacgcctttg
    30061 cgacggtcgc gtgtttgcgc gttaaccgtg gtgcgcatgg tgtaacgaat ctcttcgtcg
    30121 agctgtgcca accagtcacg aaatttccag tcatccagca gactggcttc gtgaaagaga
    30181 aactggctaa tgcggtgatg taactccagt gaaacttgcg cactcatttc atcacctcct
    30241 gctggtaagc ggcggtttta tcgagcactt cctgccagct ttcgctactc agaagatcgg
    30301 cccagcgttg gtacattcca cgagcggcag tttctgaaaa gatatagtta gtaatgccag
    30361 gaatgccgtc gtcgcggcgc ttttcctgac caagccccat ttccagacac agtttgctgt
    30421 tgcgggcgcg gtggccttta agcaatttct ggatttcaca ccagttctcc gagtcatcct
    30481 gctcgagaaa accagcagga ccaaaagcac gagtggcgct gttttcaaaa gcggctttaa
    30541 cttcatcgga ggcggctttg tcagtaatac agaacgccca cacttcaact tgatcagggc
    30601 cgcgcggatg ccagacgcgg agtgtggcag tgccgtttag ccatgaaagc gtggggaata
    30661 tattgttatg acccgccagg cgcagggcgc gaacttcacc taaacgttgt tctgcttcgg
    30721 cataggtttc gcggtaatag cttgaaactg cgccatcgac ccagacatta gcatccggtt
    30781 tttcagtaaa gaagaaaccg ctaccgtgac cgtcctgacc aaattgcagc gcatctttgg
    30841 cggtttccca caccgggcgg gcggtttgtc catcaccgag gcgcttatcg ctgccatcat
    30901 ctttcgcccc taatacctga acggcagaag catggctgaa cagagcatga tactggtcac
    30961 tggcgaactg ctctgccggg aatttccagt tacagttgat cacccacttt tgtacgccgc
    31021 cgacaatttc ggtgccgcct tcgcgacgat ccagcatgcc atccagatac caggcaatgt
    31081 cacccaggta atcatgcagg cccggtgcgc tggtatccca gttgccaaaa atcagtcctt
    31141 tataactctc cacacaagga acttcgttta gcccccagtg ggatttacac aacccttgtg
    31201 ggtaggcgcg aggttccagc ggtacatcga tcaactcgcc gttaatgcca taagaccagc
    31261 cgtgatacgg gcaggtaaag gcgcgactgt tgccacaatc tgcataactc acacgcatgg
    31321 cccggtggcg gcattggttg agaaacgcct tgatgctgcc gtctttctgg cgcaccacga
    31381 caactgcatc ttctcccatg taggtgttaa agaaatcacc gggttttggg atctggcttt
    31441 cgtgggcgag aaatagccag caacgaccga aaatacgctc aagctccagt tggtaaatgt
    31501 ccgggtcggt ataaatacgc ggagtgaccc gaccgttttg ggtgtcaatc agttggtaaa
    31561 tgttcaaatc tgagggtgtg gtcatatttt ttaatcctga aagataagat gtattgacct
    31621 tctatttttg tgccataagg atgttgctgg ttggttgcta atatgtgaaa tagttaattt
    31681 ctaccagctg agacgttttt tcgataacaa ggggaaggtg atggaactac ggcatttacg
    31741 ctatttcgtc gcagtggcgc aggcactgaa ctttacccgt gcggcggaaa aactgcatac
    31801 ctcacagcct tcgttaagca gccagatccg cgatcttgaa aactgtgtcg gtgttccgtt
    31861 actggtgagg gataagcgca aagtcgcgtt gacggcggcg ggagaatgtt ttctccagga
    31921 cgcgctggca atcctcgaac aagcggaaaa tgccaaatta cgggcgcgga aaattgttca
    31981 ggaagacaga caattaacca ttggctttgt gccatcggcg gaagtgaatt tattgccaaa
    32041 agtattaccg atgtttcgtc tcagacagcc agacaccttg attgagctgg tgagtttaat
    32101 caccacgcaa caggaggaaa aaattcgccg tggtgaactc gacgtcggct taatgcgcca
    32161 tcctgtttat agcccggaga ttgattatct ggagcttttt gacgaaccat tagtggtggt
    32221 gttaccggtt gatcaccctt tagcacatga aaaagagatc accgccgcgc aactggatgg
    32281 cgtgaatttc gtcagtaccg atccggcgta ttccggttcg cttgcgccga tcgttaaagc
    32341 gtggtttgcg caagaaaaca gccagccaaa tatcgtccag gtggcaacga atattctggt
    32401 gaccatgaat ctggtgggca tggggctggg cgtcactttg atacccggtt atatgaataa
    32461 ttttaatacc gggcaggttg tttttcgtcc cattgccgga aatgtgcctt ccattgcttt
    32521 actgatggcg tggaagaaag gggagatgaa gctggcatta cgcgatttca tcgccattgt
    32581 gcaggaacgt ttggcaagcg taacggcata attacgcatt atttttgcaa catgcgtcgc
    32641 ggatcgcagt tttttagtgc ttgtgaactg gcgttacaga catcgggaag caggctcgaa
    32701 tattcttatt tttcaacttg caccaacatt cgattaaccc cagactaacg cctcctgacg
    32761 ggagggactc atggttttgc aatccacgcg ctggttggcg ctcggctatt tcacatactt
    32821 ttttagttac ggcatttttt tacctttctg gagcgtctgg cttaaaggga ttggtttaac
    32881 gccagaaacc atcggcctgt tattgggggc aggtctggtt gcccgttttc tcgggagttt
    32941 gctcatcgcg ccccgcgtca gcgatccttc ccgcctgatt tccgccttgc gcgtgctggc
    33001 actgctgcca cttctctttg ctgtcgcctt ctgggcgggg gcgcacgtag cgtggctgat
    33061 gctggtgatg attggcttta acctcttttt ctcaccgctg gtaccgttga ccgatgcact
    33121 ggcgaatacg tggcaaaagc agttcccgct tgattacggc aaagtgcgac tgtggggctc
    33181 ggtggcgttt gtcattggct cggcgctgac gggcaaactg gtcactatgt ttgattatcg
    33241 ggtgatcctc gtgctgttga cgttgggcgt ggcatccatg ctgctcggct ttctcatccg
    33301 tccgacgatt cagccacaag gggcaagccg ccagcaggag agcaccggtt ggtctgcgtg
    33361 gttggcgctg gttcgccaga actggcgctt tctggcctgc gtttgtttat tgcagggggc
    33421 acatgcggcc tattacggtt ttagcgccat ttactggcag gcagctggct actcggcctc
    33481 ggcggtgggg tatttgtggt cgctgggcgt ggtggcggaa gtcattatct ttgcgctgag
    33541 taataaactt ttccgccgtt gtagtgctcg cgatatgctg ttgatctcgg cgatttgcgg
    33601 cgtagtgcgc tggggcatta tgggagcaac tacggcgttg ccgtggttga tagtggtgca
    33661 aattctgcat tgcggcacct tcacggtctg ccacctggcc gccatgcgtt atattgctgc
    33721 tcgccaggtt agcgaagtca tccgtttaca ggcggtttac tctgccgtcg cgatgggcgg
    33781 cagtatcgct atcatgaccg ttttcgccgg tttcctgtat caatatctgg gccacggcgt
    33841 gttctgggta atggcgctgg tggcgcttcc ggcaatgttt ttgcgcccga aagttgttcc
    33901 ctcatgctga ttcgagcatt ttgcggatct gctcctgttg atgtgttgtc agcgtcaggt
    33961 ccgtatagat cagcggtggt gaaaagagcg gtaacggcat ggtataaggc gcaataatca
    34021 gtgccgcgcc acgcggagcg cctgtctgca aaaatgcctt taccgacata tgcttaatgt
    34081 tgagcggcag taacgttagt tcgcgtaact gctgctcaat ctgcgcttct cgctcgctat
    34141 cattcccggt cagtagaata atctgttttt catgcagatc gttttcctgc attagccagg
    34201 caccgaaaat caccgcgacc agaccacttt cctcatcaga aagatggacg ccgtattcac
    34261 tttcaaatcc ggccagcgcc gcgcgggtgg tgcgtaccag gcgtggatac agtctggcga
    34321 actcttccgg cagggtatta tcaataccga tggcaaacaa actgcgattt aacgcctggg
    34381 cgaggtggat ataaagctga tcacataacc cctgttcatc ataaaaacga acatttccca
    34441 gctcacgaaa atggtttacc agacgtttga tagactgacg caattgtctg tcccgctgat
    34501 gcgcatcccg cagtggatcg ggaacccgca acatggaaaa aagtaatgcc ataaacagtg
    34561 gctcatcagg cggtacatgc tggagcgccc gacgctgcca gtggcgtcca atttcctgcg
    34621 ctacctgaaa ttcaaggcag gattccgccc agcgacgttg gagcggatta aactgaggcg
    34681 taattccagc gtggtgctgc agcaaacaat attgcagata cagacacagg aaatgaatat
    34741 cgcgcgattc aaagcgtttt tgcagccgtc gggagcagag attcactaac gcttgtagat
    34801 tggtatcgtc gtaaaagtta cgcgcaattc cgcgccgctt cagttcactc ttcagggcgg
    34861 gggtgaattg gctggtaata aatgacggac acagacggaa accacgtcgt agccagtgaa
    34921 ataaacacaa acgttggtta agcactgtac cttcaacccg gtagctaccg tcataaccag
    34981 tcgtcagtgt gagttgatga tagcgcagga tctcccgtcc tgtttcgctg atatcaagac
    35041 tggcaatatc atcatccaca ccattaagct cgctgaaggt tgccatggtg gcggtcaacc
    35101 ccggctgaaa gagcgtcagc aagatctggc agcggcgctg gggagccgaa aggacagatg
    35161 gtggagcaag cgtaggcatc atctgtagat ctcccataag cataattgat aagaatagct
    35221 aaagtttgct cagggaaatg gcgaagggaa tgctaatcat cagaaatgtt ggcaagcgtc
    35281 acagaatttt tctcatttta aactgttctg accagtggat tagcatgtct taaggtggtt
    35341 ttgtgaatga ttatcgtgct agcgatcatg ggttagttct gcggattcaa cgcgctaagg
    35401 ataaagtcca ccatctgttc gcgataaaca ttgccgtgac gatcggaaaa agcatcaatg
    35461 tgttcgccat ccgggattag gatcaaccgt tttggctctt ttgccaggct atacaacttt
    35521 tcgctgtgct gccatgggat aacgtgatcg gctttaccgt gaatgagtaa aagcgggatc
    35581 gggctgacgc tggcgatgta attttcgccg ctgtaactct catcaagtaa gtagccactg
    35641 ccggggatca tctggttggc gatggttgca taagaggcaa atgtggagtc gaggatcacc
    35701 gcacgtatgc cttcacgatc accctgacca ataacagcaa gaatattcgc cccgccaata
    35761 ctctgcccga acagcaccag ctgttgtggg tttacatcac tgcgatggcg caccacattg
    35821 atggcgcttt gcgtatcgtc cagcaatccg gcctgggacg gagtgccttt tgatttacca
    35881 aacccgcgat aatcaaacat aaaaacgttg aaattacgct caggtaacca actgaccagc
    35941 ggccagtggg cggacatatt tccggcattg ccgtgagcat gaatgatggt tgcgatggcg
    36001 ttgtcagcag ggcccgtcga agaaggaata aaccagcctt gcagacgagt accgtcctta
    36061 gccgtaaatt cgacggattc cgccgaccag gggtcgggac cgtaaatttt atcgtcagga
    36121 taatagaaga agttgatggc aacgcgcggc actaaataaa cgcagaaggc aacaacaaag
    36181 agaataaaca gaattttggg aacgcgtttg ttcactggca gtgccataac ctttccttgc
    36241 gacttcttta tcatccatta atcggggaaa gatggtggga gagtgacgaa agaaagtaaa
    36301 gcggtgagga agtatttcgg aatagcgcct gaaaggcgag ggcgggtgag tgatatcacc
    36361 cgcctgagtc attaatgctt cagagcgtcg cttaactcgt cacgcatgtt cgccagtatg
    36421 gctttcacaa cacgcgggtt accagcaacg atgttaccgg tcagcatata gttatgacca
    36481 ccggtgaagt cgctgacgat gccgcccgct tcacgaacca gcagctcgcc tgccgcgaaa
    36541 tcccacggac gcagaccgat ttcaaagaaa ccgtcaacac gacccgcagc gacgtaagcc
    36601 agatccagcg ccgcagaacc ggtacgacgg aagtctgcac attcgttgaa cagcttgccg
    36661 acgatgttga tgtaggtagt ggcgtactgt tttgctttga acgggaagcc ggtcgccaga
    36721 atggtaccgt cgagatcgcg agcggtgcta ccgcgcagac ggtagccgtt cagctgtgcg
    36781 ccttgaccgc gagtggcggt gaacagttcg ttacgcatag gatcgtatac cacagcaact
    36841 tcggtgcggc ctttgatacg aacggcgata gataccgcga agtgcggcag acgtttgata
    36901 aagttggtag taccatccag tggatcgata acccattgaa catcctgatc agtaccttca
    36961 agttcaccgc tttcttcggt gatgatggtg tgctgtgggt aagatttacg aatcgtgtcg
    37021 ataatcaccg cttcggcagc tttatctacg ttggtcacga aatcgttact gcctttctgg
    37081 ctcgcttcta cagcgtccgg ggtttcatag tttttggcaa ttaaattacc cgccttgcgc
    37141 gctgcgcgca cggcgatgtt cagcatcgga tgcatcggtc tctctcactg gatgttaaag
    37201 aacgggaaaa cggcgcagag tatagcagcg agatcggcat ctgtcttcgt tttatgataa
    37261 gatgcgtgaa taatctttag atgaagaaag acaatgctgc aaaatattcg aattgtgctg
    37321 gtggagacgt cacacaccgg caatatgggt tctgttgccc gtgccatgaa aacaatggga
    37381 ttaaccaatc tgtggctggt taatccactg gtgaaacccg actcccaggc gattgccctg
    37441 gctgcagggg ccagcgatgt gattggtaat gctcacatcg tcgatacgct cgacgaagcg
    37501 ttagccggtt gtagcctggt ggtgggcacc agcgcacgct cccgcacgct gccgtggccg
    37561 atgctcgacc cgcgcgaatg cggtctgaaa agcgtcgctg aagcggcaaa taccccggtg
    37621 gcgctggtgt ttggtcgtga gcgcgtcggc ttgaccaatg aagagttgca gaaatgccat
    37681 tatcatgtcg cgattgcggc aaacccggaa tacagttcgc tgaacctggc aatggcggtt
    37741 caggttattg cctatgaagt gcgtatggcc tggctggcaa ctcaggaaaa tggcgagcag
    37801 gtcgagcatg aagagacgcc gtatccgctg gtcgatgatc tggagcgttt ttatggtcat
    37861 ctggaacaaa cgctgctggc aaccggtttt atccgtgaaa accatccggg gcaggtgatg
    37921 aataaattgc gccgtctgtt tacccgtgcg cgcccggaaa gccaggaatt gaatatcctg
    37981 cgcgggattc tggcttctat tgagcagcag aataaaggta acaaggccga ataacagtgg
    38041 ttgcctgatg cgacgcttaa cgcgtcttat caggcctaca atgtacagaa tcgtaggtcg
    38101 gataaggtgt tcacaccgca tccgacaaca ggtacaaacg ccacgataaa aaaatggcac
    38161 tgaaggttaa atacccgact aaatcagtca agtaaatagt tgaccaattt actcgggaat
    38221 gtcagacttg accctgctat gcaatacccc cacttttaca ataaaaaacc ccgggcaggg
    38281 gcgagtttga ggtgaagtaa gacatgagac tgacatctaa agggcgctat gccgtgaccg
    38341 caatgcttga cgttgcgctc aactctgaag cgggcccggt accgttggct gatatttccg
    38401 aacgtcaggg aatttccctt tcttatctgg aacaactgtt ttcccgtctg cgtaaaaatg
    38461 gtctggtttc cagcgtacgt ggaccaggcg gtggttatct gttaggcaaa gatgccagca
    38521 gcatcgccgt tggcgaagtg attagcgccg ttgacgaatc tgtagatgcc acccgttgtc
    38581 agggtaaagg cggctgccag ggcggcgata aatgcctgac ccacgcgctg tggcgtgatt
    38641 tgagcgaccg tctcaccggt tttctcaaca acattacttt aggcgaactg gttaataacc
    38701 aggaagtgct ggatgtgtct ggtcgtcagc atactcacga cgcgccacgc acccgcacac
    38761 aagacgcgat cgacgttaag ttacgcgctt aataaaaaga attcagaatc aggccggagt
    38821 gctaaatact ccgtaaacac ggtcgtacat ccagccggta gcctgattcc ttgcattgag
    38881 tgatgtacgg agtttataga gcaatgaaat taccgattta tctcgactac tccgcaacca
    38941 cgccggtgga cccgcgtgtt gccgagaaaa tgatgcagtt catgacgatg gacggaacct
    39001 ttggtaaccc ggcctctcgt tctcaccgtt tcggctggca ggctgaagaa gcggtagata
    39061 tcgcccgtaa tcagattgcc gatctggtcg gcgctgatcc gcgtgaaatc gtctttacct
    39121 ctggtgcaac cgaatctgac aacctggcga tcaaaggtgc agccaacttt tatcagaaaa
    39181 aaggcaagca catcatcacc agcaaaaccg aacacaaagc ggtactggat acttgccgtc
    39241 agctggagcg cgaaggtttc gaagtcacct acctggcacc gcagcgtaac ggtattatcg
    39301 acctgaaaga actcgaagcg gcgatgcgtg acgacaccat ccttgtttcc atcatgcacg
    39361 taaataacga aatcggcgtg gtgcaggata tcgcggctat cggcgaaatg tgccgtgctc
    39421 gtggcattat ctatcacgtt gatgcaaccc agagcgtggg caaactgcca atcgacctga
    39481 gccagttgaa agttgacctg atgtctttct ccggtcacaa aatctatggc ccgaaaggta
    39541 tcggtgcgct gtatgtgcgt cgtaaaccgc gtgtacgcat cgaagcgcaa atgcacggcg
    39601 gcggtcacga gcgcggtatg cgttccggca ctctgcctgt tcaccagatc gtcggaatgg
    39661 gcgaagccta tcgcatcgca aaagaagaga tggcgaccga gatggaacgt ctgcgcggcc
    39721 tgcgtaaccg tctgtggaac ggcatcaaag atatcgaaga agtttacctg aacggtgacc
    39781 tggaacacgg tgcgccgaac attctcaacg tcagcttcaa ctacgttgaa ggtgagtcgc
    39841 tgattatggc gctgaaagac ctcgcagttt cttcaggttc cgcctgtacg tcagcaagcc
    39901 tcgaaccgtc ctacgtgctg cgcgcgctgg ggctgaacga cgagctggca catagctcta
    39961 tccgtttctc tttaggtcgt tttactactg aagaagagat cgactacacc atcgagttag
    40021 ttcgtaaatc catcggtcgt ctgcgtgacc tttctccgct gtgggaaatg tacaagcagg
    40081 gcgtggatct gaacagcatc gaatgggctc atcattaatc ggtatcggaa tcaggagaat
    40141 ttataatggc ttacagcgaa aaagttatcg accattacga gaatccgcgt aacgtgggtt
    40201 cctttgataa caacgacgag aacgtcggca gcggcatggt gggtgcaccg gcctgtggcg
    40261 acgtaatgaa gttgcagatt aaagtcaacg atgaaggtat cattgaagac gcgcgtttta
    40321 aaacttacgg ctgcggttcc gctatcgctt ccagctccct ggtcaccgaa tgggtgaaag
    40381 ggaagtctct cgacgaagcg caggcgatca aaaacaccga tattgctgaa gaacttgaac
    40441 tgccgccggt gaaaattcac tgttctattc tggcagaaga cgcgatcaaa gccgccattg
    40501 cggactataa aagcaaacgt gaagcaaaat aagagttgag gtttggttat gtcgattaca
    40561 ctgagcgaca gtgcagcagc gcgagtaaat acctttctgg ctaaccgcgg caaagggttt
    40621 ggcctgcgtc tgggcgtgag aacctccgga tgttcaggta tggcttatgt actggaattt
    40681 gttgacgaac cgacgccgga agacatcgtg tttgaagaca aaggcgtgaa agtcgtggtc
    40741 gatggcaaaa gcctgcaatt tctggacggt acgcagctgg acttcgtaaa agaaggcctg
    40801 aatgaagggt ttaaattcac caacccgaac gttaaagatg agtgtggttg cggcgaaagc
    40861 ttccacgttt gatgcgcata ccgataaccc caccgtggtc gcctgcgcgt ggggtttgtt
    40921 ttacctgatt cgccgttatc gcggcggatc gcagccctga gaatgttatg gattacttca
    40981 ccctctttgg cttgcctgcc cgttatcaac tcgataccca ggcactgagc ctgcgttttc
    41041 aggatctaca acgtcagtat catcctgata aattcgccag cggaagcctg gcggaacaac
    41101 tcgccgccgt acagcaatct gcaaccatta accaggcctg gcaaacgctg cgtcatccgt
    41161 taatgcgcgc ggaatatttg ctttctttac acggttttga tctcgccagc gagcagcata
    41221 ctgtgcgcga caccgcgttc ctgatggaac agttggagct gcgcgaagag ctggacgaga
    41281 tcgaacaggc gaaagatgaa gcgcggctgg aaagctttat caaacgtgtg aaaaagatgt
    41341 ttgatacccg ccatcagttg atggttgaac agttagacaa cgaggcgtgg gacgcggcgg
    41401 cggatacggt gcgtaagctg cgttttctcg ataaactgcg gagcagtgcc gaacaactcg
    41461 aagaaaaact gctcgatttt taatttctgg aagctaaaca tggccttatt acaaattagt
    41521 gaacctggtt tgagtgctgc gccgcatcag cgtcgtctgg cggctggtat tgacctgggc
    41581 acaaccaact cgctggtggc gacagtgcgc agcggtcagg ccgaaacgtt agccgaccat
    41641 gaaggccgtc acctgctgcc atctgttgtt cactatcaac agcaagggca ttcggtgggt
    41701 tatgacgcgc gtactaacgc agcgcttgac accgccaaca ccattagttc tgttaaacgc
    41761 ctgatggggc gctcgctggc tgatatccag caacgctatc cgcatctgcc ttatcaattc
    41821 caggccagcg aaaacggcct gccgatgatt gaaacggcgg cggggctact gaacccggtg
    41881 cgcgtttctg cggacatcct caaagcactg gcggcgcggg caactgaagc cctggcaggc
    41941 gagctggatg gtgtagttat caccgttccg gcgtactttg acgatgccca gcgtcagggc
    42001 accaaagacg cggcgcgtct ggcggggttg catgttctgc gcttacttaa cgaaccgact
    42061 gctgcggcta tcgcctacgg gctggattcc ggtcaggaag gcgtgatcgc tgtttatgac
    42121 ctcggtggcg ggacgtttga tatttccatt ctgcgcttaa gtcgcggcgt gtttgaagtg
    42181 ctggcaaccg gcggtgattc cgcgctcggc ggcgatgatt tcgaccatct gctggcggat
    42241 tacattcgcg agcaggcgga cattcctgat cgtagcgata accgcgttca gcgtgaactg
    42301 ctggatgcca ccattgcagc caaaatcgcg ctgagcgatg cggactccgt gaccgttaac
    42361 gttgcgggct ggcagggcga aatcagccgt gaacaattca atgaactgat cgcgccactg
    42421 gtaaaacgaa ccttactggc ttgtcgtcgc gcgctgaaag acgcgggtgt agaagctgac
    42481 gaagtgctgg aagtggtgat ggtgggcggt tctactcgcg tgccgctggt gcgtgaacgg
    42541 gtaggcgaat ttttcggtcg tccaccgctg acttccatcg acccggataa agtcgtcgct
    42601 attggcgcgg cgattcaggc ggatattctg gtgggtaaca agccagacag cgaaatgctg
    42661 ctgcttgatg tgatcccact gtcgctgggc ctcgaaacga tgggcggcct ggtggagaaa
    42721 gtgattccgc gtaataccac tattccggtg gcccgcgctc aggatttcac cacctttaaa
    42781 gatggtcaga cggcgatgtc tatccatgta atgcagggtg agcgcgaact ggtgcaggac
    42841 tgccgctcac tggcgcgttt tgcgctgcgt ggtattccgg cgctaccggc tggcggtgcg
    42901 catattcgcg tgacgttcca ggtcgatgcc gacggtcttt tgagcgtgac ggcgatggag
    42961 aaatccaccg gcgttgaggc gtctattcag gtcaaaccgt cttacggtct gaccgatagc
    43021 gaaatcgctt cgatgatcaa agactcaatg agctatgccg agcaggacgt aaaagcccgt
    43081 atgctggcag aacaaaaagt agaagcggcg cgtgtgctgg aaagtctgca cggcgcgctg
    43141 gctgctgatg ccgcgctgtt aagcgccgca gagcgtcagg tcattgacaa tgctgccgct
    43201 cacctgagtg aagtggcgca gggcgatgat gttgacgcca tcgaacaagc gattaaaaac
    43261 gtagacaaac aaacccagga tttcgccgct cgccgcatgg accagtcggt tcgtcgtgcg
    43321 ctgaaaggcc attccgtgga cgaggtttaa tatgccaaag attgttattt tgcctcatca
    43381 ggacctctgc cctgatggcg ctgttctgga agctaatagc ggtgaaacca ttctcgacgc
    43441 agcgctgcgt aacggtattg agattgaaca cgcctgtgaa aaatcctgtg cttgcaccac
    43501 ctgccactgc atcgttcgtg aaggttttga ctcactgccg gaaagctcag agcaggaaga
    43561 cgacatgctg gacaaagcct ggggactgga gccggaaagc cgtttaagct gccaggcgcg
    43621 cgtcaccgac gaagatttag tggtcgaaat cccgcgttac actatcaacc atgcgcgtga
    43681 gcattaacag aggttagtat gggacttaag tggaccgata gccgcgaaat tggcgaagca
    43741 ctgtacgatg cgtatcccga tcttgatccg aaaacggttc gattcaccga tatgcatcag
    43801 tggatttgcg atctggaaga tttcgacgac gacccgcagg catccaacga gaaaatcctc
    43861 gaagcgattt tgttagtctg gctggacgaa gccgagtgaa tgaacgggct gcctgggggt
    43921 agcccgtttt gctgttaaac gaataaggat aactaaaatg acagaagcga tgaagattac
    43981 cctctctacc caacctgccg acgcgcgctg gggagaaaaa gcaacttaca gcattaataa
    44041 tgacggcatt accctgcatt tgaacggggc agacgatctg gggctgatcc agcgtgcggc
    44101 ccgcaagatt gacggtctgg gcatcaagca tgttcagtta agcggtgaag gctgggatgc
    44161 ggatcgctgc tgggcattct ggcaaggtta taaaggcccg aaaggcacgc gtaaagtgga
    44221 gtggccggat ctggacgatg cccagcgcca ggaactggat aaccgcctga tgatcatcga
    44281 ctgggtgcgt gacaccatca acgcaccggc agaagaattg ggaccatcgc aactggcaca
    44341 gcgtgctgtt gatctgatca gcaacgtcgc gggcgatcgt gtgacttatc ggatcaccaa
    44401 aggcgaagat ctgcgtgagc aaggttatat ggggctgcac actgtcgggc gcggttcaga
    44461 acgttctccg gtattgctgg cgctggatta caacccaact ggcgataaag aagcaccggt
    44521 gtacgcgtgc ctggtaggta aaggtatcac ttttgactcc ggcggctaca gcatcaaaca
    44581 gactgcgttt atggactcga tgaagtcgga catgggcggc gcggcaacgg ttaccggggc
    44641 gctggcattt gccattatgc gcggactgaa caagcgcgtg aagctgttcc tctgctgtgc
    44701 ggataacctg attagcggca atgcgttcaa gctgggcgat atcatcacct atcgcaacgg
    44761 taaaaaagtt gaagtgatga acactgatgc cgaagggcgt ctggtgcttg ccgatggtct
    44821 gattgatgcc agtgcgcaga aaccggaaat gatcattgat gcggcgaccc tcaccggggc
    44881 ggcgaaaact gcgctgggta atgattatca cgcgctgttc agttttgacg atgcgcttgc
    44941 cggtcgttta ctggcgagtg cctcacaaga gaacgaacca ttctggcgtc tgccgctggc
    45001 ggaattccac cgcagccagc tgccgtctaa ctttgccgaa ctgaacaata ccggaagcgc
    45061 ggcgtatccg gcaggcgcga gcacggcggc gggcttcctg tcgcactttg ttgagaacta
    45121 tcagcaaggc tggctgcata tcgactgctc ggcgacttac cgtaaagcgc cggttgaaca
    45181 gtggtctgcg ggcgctacgg gacttggtgt gcgcacgata gctaatctgt taacggcgta
    45241 aactaatatg ccggatgcgg cgtaaacgcc ttatccggcc tacaaattct gcaaattcaa
    45301 caaattgcga atcccttgta ggcctgataa gcgtagcgca tcaggcattt gcgccccgaa
    45361 cgttgaatga attgtggttc ttatgtccga aacaaaaaac gaacttgaag acctgctgga
    45421 aaaagcagca accgaaccgg cgcaccgccc ggcctttttc cgtactctac tggaatccac
    45481 cgtctgggtg cctggtacgg cggcgcaggg cgaggctgtg gttgaagata gtgcgcttga
    45541 tttacagcac tgggaaaaag aagacggcac cagcgtcatt ccttttttca cctcgttaga
    45601 agcgcttcaa caggcggttg aagacgaaca ggcatttgtc gtaatgcccg ttcgcacatt
    45661 gtttgagatg acccttggcg aaacgctctt ccttaatgcc aaactgccga ccggtaaaga
    45721 atttatgccg cgtgaaatca gtttgttgat tggtgaagag ggaaatccgc tgagcagcca
    45781 ggaaatcctg gaaggcggtg aatcgctgat attatcggaa gtcgcagagc cgccagcaca
    45841 aatgattgat tcactcacca ccttatttaa aaccattaag ccggtgaagc gtgcttttat
    45901 ttgttcaatt aaagagaacg aagaggcaca gcctaattta cttattggca ttgaagccga
    45961 tggtgatatc gaagaaatta ttcaggcgac gggaagtgta gcgaccgata cattacctgg
    46021 cgatgaacca atcgatattt gtcaggtgaa aaaaggggaa aaaggaatta gccactttat
    46081 taccgaacat attgcgccat tctatgaacg tcgctggggt ggttttttgc gtgactttaa
    46141 acaaaaccgg ataatctaaa atatgccgcc ctaaagggcg gcattcgggc ttacttgctg
    46201 ctctgaaggt tgttaaccac cagtaaattg tcgccggaga gactccggtt attatttact
    46261 tgggaaaaca gcgcaccaga aacaataagt acgatacaga agcaccgtat cttcattgtg
    46321 cggtccatgc gaagaccgca tcacgacagc accactggcg atattgccgc gatagcaagc
    46381 gctgagacgc gcttgctcta acggtcgcgg aacatccttt ccttaagcca gcgcccgctt
    46441 gcgagacggc ggaaagggcc gcagtggata atacactaac agaacaataa taactctctt
    46501 ttgcttgaca aaaagagagt tactggcgag tattgtctcc gctgagacgc gcttgcatta
    46561 ctacggtaa
//
LOCUS       DAZSNO010000016        46378 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZSNO010000016 DAZSNO010000000
VERSION     DAZSNO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46378
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.016"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(87..1946)
                     /gene="gss"
                     /locus_tag="ACHWN2_000844"
     CDS             complement(87..1946)
                     /gene="gss"
                     /locus_tag="ACHWN2_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR8930486.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2151..3017
                     /gene="yghU"
                     /locus_tag="ACHWN2_000845"
     CDS             2151..3017
                     /gene="yghU"
                     /locus_tag="ACHWN2_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR8930487.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3046..3127)
                     /locus_tag="ACHWN2_000846"
                     /pseudo
     CDS             complement(3046..3127)
                     /locus_tag="ACHWN2_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3140..3388)
                     /gene="hybG"
                     /locus_tag="ACHWN2_000847"
     CDS             complement(3140..3388)
                     /gene="hybG"
                     /locus_tag="ACHWN2_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR8930488.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3522..3863)
                     /gene="hypA"
                     /locus_tag="ACHWN2_000848"
     CDS             complement(3522..3863)
                     /gene="hypA"
                     /locus_tag="ACHWN2_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR8930489.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3856..4344)
                     /gene="hybE"
                     /locus_tag="ACHWN2_000849"
     CDS             complement(3856..4344)
                     /gene="hybE"
                     /locus_tag="ACHWN2_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR8930490.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(4337..4831)
                     /locus_tag="ACHWN2_000850"
     CDS             complement(4337..4831)
                     /locus_tag="ACHWN2_000850"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR8930491.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4831..6534)
                     /gene="hybC"
                     /locus_tag="ACHWN2_000851"
     CDS             complement(4831..6534)
                     /gene="hybC"
                     /locus_tag="ACHWN2_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR8930492.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6531..7709)
                     /gene="hybB"
                     /locus_tag="ACHWN2_000852"
     CDS             complement(6531..7709)
                     /gene="hybB"
                     /locus_tag="ACHWN2_000852"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR8930493.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7699..8685)
                     /gene="hybA"
                     /locus_tag="ACHWN2_000853"
     CDS             complement(7699..8685)
                     /gene="hybA"
                     /locus_tag="ACHWN2_000853"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR8930494.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8688..9806)
                     /gene="hybO"
                     /locus_tag="ACHWN2_000854"
     CDS             complement(8688..9806)
                     /gene="hybO"
                     /locus_tag="ACHWN2_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR8930495.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9995..10282)
                     /gene="yghW"
                     /locus_tag="ACHWN2_000855"
     CDS             complement(9995..10282)
                     /gene="yghW"
                     /locus_tag="ACHWN2_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR8930496.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10401..11288)
                     /gene="yghX"
                     /locus_tag="ACHWN2_000856"
     CDS             complement(10401..11288)
                     /gene="yghX"
                     /locus_tag="ACHWN2_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR8930497.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11445..12485
                     /gene="gpr"
                     /locus_tag="ACHWN2_000857"
     CDS             11445..12485
                     /gene="gpr"
                     /locus_tag="ACHWN2_000857"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR8930498.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12525..13019)
                     /locus_tag="ACHWN2_000858"
     CDS             complement(12525..13019)
                     /locus_tag="ACHWN2_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR8930499.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13210..14094
                     /gene="yghA"
                     /locus_tag="ACHWN2_000859"
     CDS             13210..14094
                     /gene="yghA"
                     /locus_tag="ACHWN2_000859"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR8930500.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPSIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14366..14791)
                     /gene="exbD"
                     /locus_tag="ACHWN2_000860"
     CDS             complement(14366..14791)
                     /gene="exbD"
                     /locus_tag="ACHWN2_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR8930501.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14798..15532)
                     /gene="exbB"
                     /locus_tag="ACHWN2_000861"
     CDS             complement(14798..15532)
                     /gene="exbB"
                     /locus_tag="ACHWN2_000861"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR8930502.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15784..16971
                     /gene="metC"
                     /locus_tag="ACHWN2_000862"
     CDS             15784..16971
                     /gene="metC"
                     /locus_tag="ACHWN2_000862"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR8930503.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17111..17770
                     /gene="yghB"
                     /locus_tag="ACHWN2_000863"
     CDS             17111..17770
                     /gene="yghB"
                     /locus_tag="ACHWN2_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR8930504.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17810..18709)
                     /gene="yqhC"
                     /locus_tag="ACHWN2_000864"
     CDS             complement(17810..18709)
                     /gene="yqhC"
                     /locus_tag="ACHWN2_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR8930505.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18903..20066
                     /gene="yqhD"
                     /locus_tag="ACHWN2_000865"
     CDS             18903..20066
                     /gene="yqhD"
                     /locus_tag="ACHWN2_000865"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR8930506.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20171..20998
                     /gene="dkgA"
                     /locus_tag="ACHWN2_000866"
     CDS             20171..20998
                     /gene="dkgA"
                     /locus_tag="ACHWN2_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR8930507.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            21198..22124
                     /locus_tag="ACHWN2_000867"
     CDS             21198..22124
                     /locus_tag="ACHWN2_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR8930508.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22175..22432
                     /gene="yqhH"
                     /locus_tag="ACHWN2_000868"
     CDS             22175..22432
                     /gene="yqhH"
                     /locus_tag="ACHWN2_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR8930509.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22475..24694)
                     /locus_tag="ACHWN2_000869"
     CDS             complement(22475..24694)
                     /locus_tag="ACHWN2_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR8930510.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24805..26217)
                     /gene="ftsP"
                     /locus_tag="ACHWN2_000870"
     CDS             complement(24805..26217)
                     /gene="ftsP"
                     /locus_tag="ACHWN2_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR8930511.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26292..27029)
                     /gene="plsC"
                     /locus_tag="ACHWN2_000871"
                     /gene_synonym="parF"
     CDS             complement(26292..27029)
                     /gene="plsC"
                     /locus_tag="ACHWN2_000871"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR8930512.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27263..29521)
                     /gene="parC"
                     /locus_tag="ACHWN2_000872"
     CDS             complement(27263..29521)
                     /gene="parC"
                     /locus_tag="ACHWN2_000872"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR8930513.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(30067..30549)
                     /locus_tag="ACHWN2_000873"
     CDS             complement(30067..30549)
                     /locus_tag="ACHWN2_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR8930514.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSELIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30602..30994)
                     /gene="ygiW"
                     /locus_tag="ACHWN2_000874"
     CDS             complement(30602..30994)
                     /gene="ygiW"
                     /locus_tag="ACHWN2_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR8930515.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            31146..31805
                     /gene="qseB"
                     /locus_tag="ACHWN2_000875"
     CDS             31146..31805
                     /gene="qseB"
                     /locus_tag="ACHWN2_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR8930516.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31802..33076
                     /gene="qseC"
                     /locus_tag="ACHWN2_000876"
     CDS             31802..33076
                     /gene="qseC"
                     /locus_tag="ACHWN2_000876"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR8930517.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(33122..33454)
                     /locus_tag="ACHWN2_000877"
     CDS             complement(33122..33454)
                     /locus_tag="ACHWN2_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFR8930518.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            33773..34354
                     /gene="mdaB"
                     /locus_tag="ACHWN2_000878"
     CDS             33773..34354
                     /gene="mdaB"
                     /locus_tag="ACHWN2_000878"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR8930519.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34385..34699
                     /locus_tag="ACHWN2_000879"
     CDS             34385..34699
                     /locus_tag="ACHWN2_000879"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR8930520.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(34747..36639)
                     /gene="parE"
                     /locus_tag="ACHWN2_000880"
     CDS             complement(34747..36639)
                     /gene="parE"
                     /locus_tag="ACHWN2_000880"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR8930521.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(36668..37249)
                     /gene="yqiA"
                     /locus_tag="ACHWN2_000881"
     CDS             complement(36668..37249)
                     /gene="yqiA"
                     /locus_tag="ACHWN2_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR8930522.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(37249..38076)
                     /gene="cpdA"
                     /locus_tag="ACHWN2_000882"
     CDS             complement(37249..38076)
                     /gene="cpdA"
                     /locus_tag="ACHWN2_000882"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR8930523.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(38101..38523)
                     /locus_tag="ACHWN2_000883"
     CDS             complement(38101..38523)
                     /locus_tag="ACHWN2_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR8930524.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(38524..39153)
                     /gene="nudF"
                     /locus_tag="ACHWN2_000884"
     CDS             complement(38524..39153)
                     /gene="nudF"
                     /locus_tag="ACHWN2_000884"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR8930525.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            39358..40839
                     /gene="tolC"
                     /locus_tag="ACHWN2_000885"
     CDS             39358..40839
                     /gene="tolC"
                     /locus_tag="ACHWN2_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR8930526.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40987..41658
                     /locus_tag="ACHWN2_000886"
     CDS             40987..41658
                     /locus_tag="ACHWN2_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR8930527.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            41664..42824
                     /locus_tag="ACHWN2_000887"
     CDS             41664..42824
                     /locus_tag="ACHWN2_000887"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR8930528.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(42862..43650)
                     /gene="ygiD"
                     /locus_tag="ACHWN2_000888"
     CDS             complement(42862..43650)
                     /gene="ygiD"
                     /locus_tag="ACHWN2_000888"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR8930529.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     LNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43793..44566
                     /gene="zupT"
                     /locus_tag="ACHWN2_000889"
     CDS             43793..44566
                     /gene="zupT"
                     /locus_tag="ACHWN2_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR8930530.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(44624..44794)
                     /gene="yqiD"
                     /locus_tag="ACHWN2_000890"
     CDS             complement(44624..44794)
                     /gene="yqiD"
                     /locus_tag="ACHWN2_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR8930531.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(45055..45708)
                     /gene="ribB"
                     /locus_tag="ACHWN2_000891"
     CDS             complement(45055..45708)
                     /gene="ribB"
                     /locus_tag="ACHWN2_000891"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR8930532.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45812..45960)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            46082..46372
                     /gene="ubiK"
                     /locus_tag="ACHWN2_000892"
     CDS             46082..46372
                     /gene="ubiK"
                     /locus_tag="ACHWN2_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR8930533.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11526 a        12398 c        11565 g        10889 t
ORIGIN      
        1 tattggaata tataaatatt gaatattttg attaatcccc gtactaatta ttcttcataa
       61 tcagtacggg gttgctacaa catgtattac tttttcacca caattaacgg ttcaatatca
      121 ctctcttttt tgatgaccag tgattcatca ccgcgcaaac acgtcccacc gtagttgccg
      181 ccaacggtga aggtacatac ctgaatgtat ttaccgtcca ctttcggcaa acaccacagt
      241 tgctgataga tgtttttctg ctcggcaaat ttaccgctgg ttttgtccag cacctcttca
      301 tgatggctga ccaggtcgat attgctacca cagcgaccgg cgatcggttt cactgcgtaa
      361 cctgttttca ccaattcatc attaacagtg aaatcggtat ccagcaggta acgatggtgc
      421 gggaacagcg accagaggat cggcagaatc gctttgttgc cggggatcac cgtccacagc
      481 ggctcaaaga ccagcacttc cgggcgcagc aatacgtcga taagacgcac ttcgttttgc
      541 ggatgaccgg tacggattgg caccgcagca aactcacggt cgctaacttc acgaatctga
      601 tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt taaccagtcg cccttcccca
      661 tcaatcagtt gcccggcagc atcccagccc agttcatcca gcccgcgcaa gatacgcgtt
      721 tcaaagcccg cctggtgcag cgcctgctcc ataaactgcg cgtgatagtt ttcctcgata
      781 tctttgtcct gcatgatatg gacaaacgga cgtgcacgac tgtgtttcca ggcaccagcc
      841 agttcgttaa tcagcccttc cgccggattg aagccgttgc ctttatagcc ctgctccgcc
      901 caacgttcga ggatcaagcc cgcttcggta tgacaggagg cggagtcggc gttgtattcg
      961 taaaccttca ggccacgctc atccatgcag aaatccatac gaccagtgat catatggtga
     1021 cggcgacgct gccaggagag acgcaaacgt ggccagagga ttttcggaat gtcgaacagc
     1081 gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt gaagatacat cagatgcagc
     1141 tcgttggtgg ctttaattag ctcctgctcg gcactctcgg taatggtgta gtaatgatac
     1201 ggatcctgat tgatcacctg accgttggcc tgcacatagg cgttttgtag cggatctttt
     1261 tcatccagcc atttaccgtc aaactggcct ttgttttcca gacgcgctcc actgattttc
     1321 agcagctcgc ctgcaatttc cggctgcggt aagctgtatt cagtatcttc cgtctggatc
     1381 atccagccca gaatggtggt gtcatcaaaa gtgtctttaa gggtatagca gccgttttcg
     1441 accaccatct ccagctcgcg cgtccactgt tgcccttgcg gcaacgggga atgaatcacg
     1501 ttctgttccg caatacggac tttgttgcca tgcaattggg taatgatggc gacatggcca
     1561 gtgtctttaa attcaccgcc tttatcccag ataagaagcg cacccgcgac cggcgcacgc
     1621 ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt cattaaccac ttctcgcagg
     1681 aagcgcagcg agaaaatctc ccacgccata cccacgtcag taaagaccac accgtaattc
     1741 agaaagagaa aacggcgagc aaattcaacg cattgccact tgtggcccat atattcgtcg
     1801 tcgatatagc tacggaatac ggcgtcatct tcgtattcct gcggatcgag agaactgtaa
     1861 tctgaagagt agattgctac cccacctggg gcgtagccca ataatgtccc gaacggggca
     1921 tcctggctgg tcgttccttt gctcatcact ttacctttaa caatacaacc taagcagttg
     1981 gcgtaattgt tgtgctctga ttacctgctc agcgacatta accggacaga ggtcagacta
     2041 atcatacacc tcatcgcagc ggctggcgca aagggttaaa aaattcacat tctgtacagc
     2101 aagtgacctg ctacactgct tcaacactac cactcagaag gcaactcact atgacagaca
     2161 atacttatca gcccgcgaaa gtctggacgt gggataaatc cgctggcggc gcgttcgcca
     2221 atatcaatcg cccgttttct ggtccgacgc atgaaaaaac gctgcccgtt ggcaaacatc
     2281 cattgcaact ttattcgctg ggaacgccga acggtcagaa agtaacgatt atgcttgagg
     2341 agctactggc gctgggcgtt actggtgcag agtacgacgc ctggctgatt cgtattggcg
     2401 atggcgatca attctccagc ggctttgtcg aagtgaaccc aaactcgaag atcccggcgc
     2461 tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga atctggttcg atcctgcttt
     2521 atctggcgga gaaatttggc tacttcctgc cgcaggatct ggcaaaacgg actgaaacgt
     2581 tgaactggct gttctggtta cagggtgccg cgccgttcct cggcggtggt tttggtcact
     2641 tttaccatta cgcgccggtg aaaattgagt acgccatcaa ccgctttacc atggaagcca
     2701 agcgcctgct cgacgtgctg gataagcaac tggcgcagca taagtttgtt gcgggcgatg
     2761 agtacaccat tgcggatatg gcgatttggc cgtggtttgg caacgtggtg ctcggcggcg
     2821 tgtatgatgc cgcagagttt ctggatgcgg gaagttataa acatgtgcaa cgctgggcga
     2881 aagaagtggg cgaacgtccg gcagtgaaac gtgggcgtat tgttaaccgc accaacgggc
     2941 cgctgaacga gcagttgcat gagcgccatg acgccagtga ttttgagaca aatacagaag
     3001 ataagcgtca ggggtaaggg ttggtgttcg tcgcagcaag ccatccaggc cggataaggc
     3061 gttcacgccg catccggcaa tcgtgcacaa tgcctgatgc gacgctgccg cgtcttatca
     3121 ggcctacaaa agctaatcat cacgcgctgg taatgtcgta atccatttgg cgcagtgcgt
     3181 ctaatgtggc tttggcttca tcttcgtcga tgatgctcat ggcaaatccg acgtgtacca
     3241 gcacccactg gcccagcaga tcggcagggt taccttcgca aatcagggcg atattcacgt
     3301 cgcgcttgat accacatact tcaacctgcg caagctggtg aatatcttca ccgacagcca
     3361 gcacctggcc tggaacgcca atacacattg ttaactccgg ttatttcgat tgccggacaa
     3421 acgcgaaaaa gaacctttag cgggtgttcc ctctcaccct aaccctctcc ccggaggggc
     3481 gaggggaccg tccgtgcgct ttttaggcgt tgtcctcagt cttattcaac ttcaatactt
     3541 ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac
     3601 tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat cccagcacca ggcctgggcg
     3661 ggtttataga cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca
     3721 aaactaaaac ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg
     3781 gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc
     3841 tggcaaagcg acaactcatg cattttcgcc cctcctgcgg ccaaacagca gggcgcgacg
     3901 ccctgcgtgt ggtacatccg gattggtgac tggcaacgaa aggatcattc gtgcgcagtc
     3961 atccgtcagg cgttggcctt cttcaatcga catgctgtgc gaaagcggcg acatcagcga
     4021 acaggagagg tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata
     4081 cggcagttgc agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc
     4141 ggggaagata actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc
     4201 gaacagcgta aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg
     4261 catcgaacgc cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg
     4321 gaaacctgct atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt
     4381 cgcgcagcgc agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg
     4441 ttaagccgat gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg
     4501 gaaactcacc ggtgaagcgc agggccgaca atacgtcggc caggccaagc tgatgcggag
     4561 agattttgtt ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg
     4621 gagtgttctt tttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt
     4681 cgccaagcag ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa
     4741 tgtatcgttg ctctaacgct tcgacaatcc gcacgccgat ggcttcatcg gtcagcaaaa
     4801 tattgccgac ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt
     4861 gccgtcggca tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt
     4921 acgcaccact tccagcggtt tattcggatc ggcaaccggt gtacccacca gcgactgctc
     4981 gtaaggaccg acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac
     5041 cgcctggtag ttgctgatga taccgtcttt aataaccatc cagtgagaga gcataccgcg
     5101 cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggcttcac
     5161 aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag
     5221 gatatcctgc aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg
     5281 cagctgtgcc acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt
     5341 cagtttgttc tgggtagatt cgcgacctgc cgccagtttc accagcatat tagccagcgg
     5401 acccacttct accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc
     5461 gtcagaccaa ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc
     5521 gtctttatac caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga
     5581 atgagaagtg atcggacgat acgaggacag atccgcattc tcaatgtagc ctcccgggaa
     5641 caggaagctg ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc
     5701 acctttaccg cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt
     5761 ataaacctgc tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg
     5821 ctcaaggttc aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccacagc
     5881 caggttctga atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg
     5941 ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag
     6001 tttcatcgcc gggtgacccc agtagccatt ggcgaaaata cccaactgac cgctggcaac
     6061 cagatctttg atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt
     6121 cgaaacgcct ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat
     6181 gtccacccag tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg
     6241 cgcagccaga atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc
     6301 actttctgcc gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg
     6361 ttgcacaatc atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg
     6421 ccacatggta ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat
     6481 gcgtaaatgc ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg
     6541 ctcgcctcat gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga
     6601 atgagtacga tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct
     6661 tcccaggtcg ggaagtaggc gtaaccgccg cccgggttga atgccaccaa cgaataggtc
     6721 agacgccagg ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catacgggaa
     6781 tcattacgca gcttcgccac acgcagaacg accagcggga agagcatcag caggacttca
     6841 atccagaaca tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag
     6901 ataagctcgc caaagcgcag cccgatgaaa atcgccagca acacactgat ggtgttggtc
     6961 agcttgacga acaggctctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagt
     7021 gaaccttcaa agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc
     7081 ggcaacattt catagctctg ccagagcgga tgcaccttgt agcccgccga gatcatcagc
     7141 gaccccattg aagactggtg catggtcggc agcagcgcac cgagcgcgat gatgaagaac
     7201 atcaccttgt tcagacgttg tagcgacacc ttccagccca gacgttcaaa cagtgccgga
     7261 gcaaactcca gagccatcac gccgatgtag atggtcatac agaccgccgt ctcgaacagt
     7321 accgagttca cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc
     7381 acgtcgatag tgatcgacaa gccacccagt gagtaaccaa acagactcgc caacagcgcc
     7441 ggacgcacca gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc
     7501 cagccgccac aggcaaagcc ggtgccaatc agcaggtcaa acgcgatcca cacgccccac
     7561 gggaagccgc cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc
     7621 aggagcatac agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg
     7681 ccgcccagcg gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt
     7741 ggtgttgcga cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc
     7801 tttatacagg gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt
     7861 cggcaggtcg agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc
     7921 ctctttctcg ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc
     7981 agatttcagc gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt
     8041 tttcgcctcc gccatcagct cttcacgcgt accgaaaatc accgcgcccg ccgggcacac
     8101 ttctacgcag ccaggcagac cgcctttatc gagacgttcc acacctttct ggttgcacag
     8161 ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtacgg
     8221 acaggcgacc atgcagtaac ggcaaccggt gcacacgtct ttgtcgtaat ggacaatgcc
     8281 ggttttcgga tcttttttca gtgcagagac cgggcacaca gagacacagt tcggatcgac
     8341 gcagtgcata cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac
     8401 ccctgtgccg ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt
     8461 gttcgaccag gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt
     8521 ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc
     8581 cggaattggc gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag
     8641 caatgccccg caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt
     8701 gagtcagcgt tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca
     8761 ccggcaacca gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg
     8821 ccctctttag cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca
     8881 agctgatgga tgcctttatg gaagccgata cctttttcgt tacagccata gcacgggtga
     8941 ccaatcgcca ccggccacac accgccaaca tcgcagaatt gcagcgttga gcagttgccg
     9001 taagtttctg gccctttaca gccgaggtgg tacaagcacc agccttcgcg gtggccttca
     9061 tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg
     9121 tgaatcagac ggccataggc gaaggtcgga cggtttttgt catccagttt cggcggtttg
     9181 ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc
     9241 ggaatattga taacggtttt gcctggcaga acttcttgca agctgactgc gccagttggg
     9301 ttaactccag ctgcggcaac accgccccac gcggagcagg aaccaatagc gataatggct
     9361 gctgagcctt ccgccgcttt gcggatgtga tccacaatcg gctcaccggc aaccatgcaa
     9421 taaataccgt tatcttttaa tgggatggaa ccatccacca ctaacacata ctgccctttg
     9481 tacttctcga gagcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc
     9541 acttcgtgat attccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt
     9601 gcacgaagca gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc
     9661 ggacgctgcg ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc
     9721 atggtggcgg ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa
     9781 tggatgaggg tgttatctcc agtcatttat agttattccg ttgcgagaac ctggcatata
     9841 ttttgcctca atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta
     9901 tggttatcga tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg
     9961 taattaatta aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca
    10021 taaaaaagcg caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt
    10081 tatccagtcc atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc
    10141 cggtcttttc gtacatcctg tgtgagtagc ctaatacaaa accgcgttta taatcggcac
    10201 agaattttgt cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc
    10261 ctttaccaaa atggttattc atcgttcacc tcattcatta tatatcgcca tatataacga
    10321 tattttaggc aaagaacgac ttttgcgcac gctatttttt catacttgtt taagacgtaa
    10381 tcccgtaaga gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc
    10441 caggcaagat cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga
    10501 ttaacccccg gatagatata agcctcataa accttattat tgtctttcaa cgccgcctcg
    10561 taaggaggcc agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc
    10621 gcttcaatct tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag
    10681 gccagttccg gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg
    10741 gtaataccca ctttacctgt cgcttgcgga tagcgttgca taaactcaat tgcggcaaag
    10801 aaatcattca tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca
    10861 tttcccggat aacctccaac ggaacttaat ccgtcaggtg ccagggcgat ataccccgcc
    10921 ttcgccactc gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc
    10981 acccccacgg ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc
    11041 tcgccgtgac catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta
    11101 aactctacct gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc
    11161 gtcatcccgc ccaccgcata cttcgccgca aggttgagga actcacgttt cgaaattttc
    11221 ccgtgaacgt aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa
    11281 cgcggcatcg ttgcattcct caactggtgt ttttttaagc aaagcataag cgcgtgtttt
    11341 tgcccagttt ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa
    11401 actagaaaca ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga
    11461 atcccgaacg ttacgggcag atgcagtacc gctattgcgg aaaaagtggt ttacgcctgc
    11521 ccgcattatc gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc
    11581 gtgcaatcct gcgtaaagcg tttgatttag gcattacgca ctttgattta gccaacaatt
    11641 acgggccgcc tccaggaagc gcagaagaga actttggtcg cctgctgcgc gaggattttg
    11701 ctgcttatcg cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc
    11761 cgtacggctc tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc
    11821 gtatggggct tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga
    11881 tggaagaaac cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg
    11941 ggatctcctc ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga
    12001 aaattccgct tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa
    12061 gcggcctgct ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg
    12121 ctcagggatt gctgaccgga aaatatctca acggcattcc gcaagattca cggatgcatc
    12181 gtgaagggaa taaagttcgt ggtctgacgc cgaaaatgct taccgaagcc aacctcaaca
    12241 gcctgcgctt attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt
    12301 taagctggtt gctgaaagat gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg
    12361 agcaactaga ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg
    12421 cgcagattga tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata
    12481 aatgacctgt taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga
    12541 gtcagtcggt caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg
    12601 acgtaccaca tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtaag
    12661 tgaatggaag aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc
    12721 attttcccca gccacttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa
    12781 ttctcataac cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc
    12841 agcgacagca acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg
    12901 tgaataatct cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag
    12961 tacacggggg caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata
    13021 cattcctgtc gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca
    13081 acagcaaatc aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc
    13141 ctggttccct tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg
    13201 agaaaaacga tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc
    13261 aaacagaaac aaccgacgcc aagcatccag gcgaagatga caccgatacc ggattgcggc
    13321 gagaaaacct atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc
    13381 gattccggaa taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg
    13441 atcagttatc ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa
    13501 tgcggacgca aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg
    13561 ctggttcacg aagcgcacaa ggcgttaggc gggctggata ttatggcgct ggtcgccggg
    13621 aaacaggttg ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt
    13681 gccattaacg ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa
    13741 ggtgcaagtg tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg
    13801 gactatgcgg ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc
    13861 gcggagaaag gtattcgggt gaatattgtc gcgccaggcc ctatctggac ggcactgcaa
    13921 atttccggcg gacaaacgca ggataagatc ccgcagtttg gtcagaaaac gccgatgaag
    13981 cgtgcggggc aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg
    14041 agctacgtca ccgcagaagt gcacggcgtg tgcggcggcg agcatttagg ttaaaaaaat
    14101 gcccggttgt gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa
    14161 aacgccttat ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc
    14221 ctgataagcg tagcgcatca ggcattgagc acctaatgcc ggatgcgtcg tgaacgcctt
    14281 atccggccta caaacgcacg aagattcaac atgttgcaat agattttgta ggcctgatga
    14341 gcgtagcgca tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac
    14401 ctatcttcag gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat
    14461 cgacggtttt gtccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat
    14521 tcaacgccgt aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt
    14581 ctgccttcac cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag
    14641 caggcaagtt caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca
    14701 gcagaaccaa catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat
    14761 cgtcgaggtt ttcgttaaga tgcattgcca tcggacatta tcctgcgcgt aatttttgtg
    14821 cgacacgaac cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca
    14881 acaatacctg cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa
    14941 atacgttata gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca
    15001 gagcttctgc gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac
    15061 caataaagct gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg
    15121 cgccgatggt tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc
    15181 gacgctccag acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg
    15241 acagttccag ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa
    15301 aatcagcggc gatatcgttg gcctggttta aggaacgtgc ttcagccagc agttgctgct
    15361 cgcgcttaag gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc
    15421 aggtgactac agaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt
    15481 actgatacat accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc
    15541 tccaggacgc aagtcacaaa atctgcgcat aataatatca aaacgacgtc gaattgatag
    15601 tcgttctcat tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc
    15661 agtaaaaaag tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag
    15721 gatgcgcccg cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc
    15781 gacatggcgg acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac
    15841 actcttggcg cggtaaatag cgtgattcag cgcgcttctt cgctggtatt tgagagtatg
    15901 gaagccaaaa agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc
    15961 ggaacgttaa cccatttttc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc
    16021 tgcgcgctat ttccctgcgg ggcggcagcg gttgctaatt cgattcttgc ttttgtcgaa
    16081 cagggcgatc atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc
    16141 aaaatcctca gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat
    16201 atcgttaagc atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc gggttccatc
    16261 actatggaag tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtgccggat
    16321 gccatcatta tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt
    16381 ggcatcgatg tttctattca ggccgccacc aaatatctgg ttgggcattc agatgcgatg
    16441 attggcactg ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa tgcctatctg
    16501 atgggccaga tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta
    16561 ggtgtgcgtt tgcgtcaaca tcatgaaagc agtctgaaag tggccgaatg gctggcagaa
    16621 catccgcaag ttgcgcgagt taaccaccct gccctgcctg gcagtaaagg acacgaattc
    16681 tggaaacgag actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc
    16741 aatgatgaag agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg
    16801 tggggcgggt atgaatcgtt gatcctggca aatcaaccag aacatatcgc cgccattcgc
    16861 ccacaaggcg agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat
    16921 gtcgacgatc tgattgccga tctggacgcc ggttttgcac ggattgtata acattgccac
    16981 ttttggacaa ttttgcagac attttattgt gaaaagtctt aaattgttgc gtccgggatc
    17041 aaggcgtccc ggacgattca ggagtacaat aggcagataa aggcttaaac gctgttccac
    17101 aggaaagtcc atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc
    17161 cgcgctggcg gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt
    17221 tttagaaaac ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc
    17281 aggcgcattg attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc
    17341 cgcagcaagt ctgggctgct ggctaagtta tattcagggg agctggttag ggaataccaa
    17401 aacggtgaaa ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat
    17461 gtttgaccgc cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac
    17521 gctgctgcca accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa
    17581 ctggttaagt ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat
    17641 gattccgttc gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat
    17701 tgccttgtta accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg
    17761 taacgcctga cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct
    17821 gcatcgcccg cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact
    17881 cacggctaaa ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca
    17941 tgccgtcatg gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca
    18001 acggcgaggt gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg
    18061 cttctgccgc cagttgctcg acgctcaggt tttcggtgta tttattctca attcgtttca
    18121 gcacgcggct aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc
    18181 cgcaaggtcc ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac
    18241 gcgcatccag tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt
    18301 ctgataacgt ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt
    18361 cgccaatgtc catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg
    18421 ccagcggcac ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca
    18481 gcaggtattc attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc
    18541 cggaaaagag aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct
    18601 cgccatacaa caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca
    18661 gtttattaac tttatccgcc agcaagcgac aaatctcttc acgtttcata tcgcgtaatt
    18721 tcttaggaat aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct
    18781 ggagaaatag gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac
    18841 aatgaaaaac atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag
    18901 taatgaacaa ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg
    18961 ctggtttacg cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca
    19021 gcgtgaaaaa aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc
    19081 tggaatttgg cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac
    19141 tggttcgcga acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg
    19201 gcaccaaatt tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc
    19261 tgcaaacggg cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc
    19321 cagcaaccgg ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca
    19381 agcaggcgtt ccattctgca catgttcagc cggtatttgc cgtgctcgat ccggtttata
    19441 cctacaccct gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg
    19501 tggaacagta tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca
    19561 ttttgctgac gctaatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg
    19621 tgcgcgccaa cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatt ggcactggcg
    19681 taccgcagga ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg
    19741 atcacgcgca aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca
    19801 agcgcgctaa gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgacg
    19861 atgagcgtat tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc
    19921 cgacccacct ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac
    19981 tggaagagca cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc
    20041 gccgtatata cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat
    20101 ttcgtccaga cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa
    20161 ggaggaacgt atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca
    20221 gctgggactg ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc
    20281 gttagaagtg ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt
    20341 cggcaaagcc ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct
    20401 gtggaacgac gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca
    20461 gcttgattat atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt
    20521 cgaagcatgg aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt
    20581 gtgcaacttc cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt
    20641 gataaaccag atcgaacttc atccgctgat gcaacaacgc cagctacacg cttggaacgc
    20701 gacacacaaa atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt
    20761 cgatcagaaa gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt
    20821 tatccgctgg catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg
    20881 tattgccgaa aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcaaaat
    20941 tgcaaaactc gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaacg
    21001 tgcaaattct cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc
    21061 ccctcgcgtc ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc
    21121 atcatgcaga gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac
    21181 ggagaggtga aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta
    21241 ccgtacacgc gcagcctcct tcacagaccg tagaacaaac agtccggcag atttatcaga
    21301 actataaatc agatgccagt acgccttatt tcggtgaaac cggagagcgg gcgataactt
    21361 cagcgcgtat tcaacaggcg cttaccctga acgacaatct tacactaccg ggcaatattg
    21421 gctggctgga ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgttagaaa
    21481 gcgtagcgat aacccaaact gacgtcgatc atgccgatgc cgttgtgcgc tttcgtatct
    21541 ttaaagatga taaagaaaag accacacaga cactgaaaat ggtggcggaa aatggtcgtt
    21601 gggtcattga tgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga
    21661 atgaaaaaac gctggccgct ttagcttcgt tgcagaaaga acagccagaa tcctttgttg
    21721 ctgaactctt tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact
    21781 cttaccgcca ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg
    21841 atgaagatat gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag
    21901 agtcgctatt ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc
    21961 gcattcatgt tcgttttacg ctgaccaatg gcaacaatga agagcaagag ctggttttac
    22021 agcggcgcga aggtaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac
    22081 ttaagcagat tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca
    22141 attagccgga acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc
    22201 tgttgttctg gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcaact
    22261 ggcgagcaat gtacaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc
    22321 actacgccca caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga
    22381 tgcacaggac tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa
    22441 tccccggcag catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc
    22501 gtttcttcgc accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag
    22561 caggcgtctg acgctgagtc gccatcggcg tatgtttagt caacgccgga cgggtattgc
    22621 ggttctggcg acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc
    22681 gacggctgcc aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc
    22741 agtttgccgg atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct
    22801 tcggtacgaa gacgccttcg ctcttataac caatcttcgc cagcggtttt ttcccggtgt
    22861 aatacatggt ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac
    22921 ggaaacgatg ctttttcagc cacagcgcca aattcaccat atcttcatca cgcgtaccgg
    22981 ggtgcgcaga gatgaaatac gggatcagat actgctcttt acctgcctgt ttcgagtagg
    23041 tatcgaacag ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgaca
    23101 acggcccttc ttcggtatgt tccggagcaa tcttcagata accgccgacg tgatgggtcg
    23161 ccaactcttt gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga
    23221 taaggatttt tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt
    23281 cgtggttagt gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag
    23341 tttgttcagc gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga
    23401 gatcggaaat cacgcccgta aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa
    23461 tgatcgaatc ttccgaacgg ctctgaataa tgcgcccttc gtgctcggta atagaacaga
    23521 aagaacagcc gccaaagcag ccgcgcataa tgttgaccga aaaacggatc atttcgtaag
    23581 ccggaatacg ggcattgcca taggccggat gtggcacgcg cttgtacggc agcgcaaaaa
    23641 cgctgtccat ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt
    23701 caccgtgttt ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc
    23761 gcgaagcatg ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa ggcagcaata
    23821 cgtaggtttt ttcccacggt ttctggcgcg gtggctgcac gattacagct ttcgcttcct
    23881 gctttttcgg cgcaacaggt ttgttatccg cacacggcaa atcttcgcca tacggatgcg
    23941 ggattgggtc gattttccct agggtatcaa gacgggtgga atccacaccg ctccagccag
    24001 gcagcgcctc ttttacgata attgcggtat tacgcacatc gcggatttca ctaatcggct
    24061 cgcccatcgc cagacgatgc gctacttcca ccagcggacg ttcaccgtta ccaaacatca
    24121 gcatgtcggc tttcgaatct accagaacgg aacggcgcac ggtatcggac cagtaatcat
    24181 aatgcgcggt acggcgcagg ctagcctcaa taccgccgag gatcaccggc acatctttcc
    24241 acgcctcttt acaacgctgg gtataaacca gcgtggcgcg atccggtcgc ttacccgcga
    24301 cattatctgg cgtgtaggca tcatcatgac gtaaacggcg atcggcggta tagcggttga
    24361 tcatcgaatc catgttgcca gcagtaacac caaaaaacag attcggttta cccagacgca
    24421 taaagtcgtc tttgctgctc cagtccggct gggcgatgat cccgacgcga aagccctgcg
    24481 cttccagcat acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc
    24541 cagtaaccaa aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca
    24601 tcggcaaaaa cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt
    24661 cgcgatccgg ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat
    24721 aatcaaaggc cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc
    24781 gccctcctcg ggcgagtatg aagattacgg taccggattg accaacagtt gcccaatcga
    24841 gccacggtcc gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga
    24901 aggctgaccg aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca
    24961 gcctctgtct tccgggaacg gcatcgcgcc attcacgtta cggatctgga acattacgcc
    25021 ttcaatatgg aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg
    25081 ctgcgcggtg acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc
    25141 acccagactg atatcgcgac tgcgaattgg cgaaccagcc atgatttcag ttggcagcaa
    25201 gcgcatcgga agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac
    25261 caaggtagaa accagaatac tggatggctc aaagaagccg cgaatacgat caacaatgct
    25321 cgccgcttca ccacaggtga tcgacacttc atcgccgttg ctcatatcca ccagaatctc
    25381 gcggcgctcg cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc
    25441 ctgatcgcca gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg
    25501 agagttcgac gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg
    25561 gctttgtaca ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt
    25621 gtattctggc gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc
    25681 cacaccataa tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat
    25741 tccggcaagg ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca
    25801 cagagtagct gcgttctggc gaataggcag tacgggtgcc cagtcagcgt ttggcgacat
    25861 catccgtgcc ggaccgccca tcagcgggcc tggtacctgt agcccggcca ccgtcattga
    25921 gacattttct gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg
    25981 gatagtcggc cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt
    26041 aaatgaccag tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag
    26101 tagcggcgga acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc
    26161 gcctgcacaa agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt
    26221 tgctccatgt aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac
    26281 agattgccga cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg
    26341 gcgatttttt gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg
    26401 ccatactgac tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt
    26461 cgattaagat taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc
    26521 gccgcaattg ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg
    26581 cgggttcctt ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact
    26641 tccgcaatgg tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt
    26701 aaccagtaca actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc
    26761 gtcggcggtt gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg
    26821 atatagatag cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg
    26881 ccgaacagtg gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta
    26941 cgcgggctga aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg
    27001 gtaataataa gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat
    27061 tgtacgtcac ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat
    27121 tgttaatcgc aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg
    27181 ttgaatacgc tgccggatga cgacttaacg tttcatccgg cgttccttgc tagcgggagg
    27241 aaacagcgcc ctccccggca tattactctt cgctatcacc gctgctggca cggcgaggag
    27301 agtcgatctc aacacgatcg atacgctgca aaccgcgcat caacgtaccg cggcgtccac
    27361 gttcgccagt gactttctgt agctcttccg gacgcagttt aattttgcgt ttcccaacat
    27421 gaatggtcag cgtgctttgc ggcggcagaa cgtacagttg cgccagacca tcctcgccac
    27481 gcgcggcttc tgccgatgga atgttgataa tcttgttgcc tttgcccttc gacagctgcg
    27541 gcagatcgct taccgggaac atcaacatac ggcctgcctg agtgattgcc agcagcatat
    27601 cggaagcatc ttcaatcacc accggcggca taacatgggc attttccggt aaggtgatca
    27661 aagccttacc cgcacggtta cgcgctacca gatcgttaaa ggtgcagacg aaaccgtaac
    27721 ccgcatcgga agccatcagc agtttctgat cgtcgctttc catcagcata tggtcaacgg
    27781 tcgccccagg cggcaacgtt aatttgccgg tgagtggttc gccctgacca cgcgccgacg
    27841 gcagcgtaat cgggtcgatg gcatagctac gaccggtgga atcaacaaac actaccggtt
    27901 ggttactctt acctttcacc gccgctttga agctatcacc cgctttataa ttcaggcccg
    27961 gcgcgtcgat atcatggcct ttagcgctgc gtacccagcc catctgcgac agcacaatgg
    28021 tgacaggttc agacggcagc atgtcgtgct cgctcatcgc tttcgcttct tcgcgttcct
    28081 gcaacggcga acgacgatcg tcaccgtagg cttgcgcgtc tgcctgcagt tctttcttca
    28141 gcaggttatt cattttacgc tcggaagcca aaatgccctg caactggtcg cgctcttttt
    28201 ccagctcact ctgctcaccg cgaatcttca tctcttccag tttggcaaga tgacgcagtt
    28261 tcagttcgag gatcgcttcc gcctgggttt ccgtaaggcc aaaccgcgac atcagcgccg
    28321 gtttcggttc atcttcgtta cgaatgatct caatcacttc gtcgatgttg agaaacgcca
    28381 ccagcaaacc ttccaggata tgcaggcgct tgaggacttt ctccagacga tagttcagtc
    28441 ggcggcgcac ggtatcgcga cggaacacca gccattcgga gaggatttcc agcaggtttt
    28501 tcaccgccgg acgaccatcc agaccgatca tattgaggtt aatgcgatag ctcttttcca
    28561 gatcggtggt agcgaagagg tggttcatca cctgatccat atccacgcgg ttggaacgcg
    28621 gcacaatcac cagacgggtc gggttctcgt ggtcagattc atcgcgcaga tcgtcaacca
    28681 tcggcagctt tttgttgcgc atttgcgcag caatttgctc cagtacgcgc gcacctgaaa
    28741 cctgatgcgg taatgcgctg ataaccaccg cgccatcttc tttcttccac accgcgcgca
    28801 tacgcactga accacgtccg ttctcgtaga ttttacgaat ctcggcgcgc gaagtgataa
    28861 tttccgcttc agtcggataa tccggcccct gcacgatatc cagcagctga tcgagcgtag
    28921 ttttcggctg gtcgattaat gcgattgccg cctgagctac ttcacgcagg ttatgcggtg
    28981 gaatatcggt cgccatgccg acggcaatac cggtggtgcc gttaagcaaa atgtttggca
    29041 gacgggcagg tagcattttc ggctcctgca aagtgccgtc gaagtttggc acccagtcag
    29101 ccgtcccctg ccccagctcg ctcaatagca gctcggaata tttcgacaac cgggattcgg
    29161 tgtaacgcat tgccgcgaac gatttcggat cgtccggcgc gccccagttc ccctgaccat
    29221 caaccagcgg ataacggtaa gagaacggct gcgccatcag gaccatcgct tcataacagg
    29281 cgctatcgcc gtgcggatgg tatttaccca gtacgtcacc gacggtacgg gccgattttt
    29341 taaatttggc gctggcattc aggcccagtt cagacatcgc atacacaatg cggcgctgaa
    29401 caggtttcag accatcacca ataaacggca acgcacggtc catgatcacg tacatggagt
    29461 agtttaagta ggcgttttcc gtaaattcat gtagcgcaag gcgctctgcc atatcgctca
    29521 ttaattctga ttcctcaact tattcgccag ccttcaaagg ggcaatattg ccggagatac
    29581 tacattatct gccgcgtcga gtcacaggag atcagcgtga atagcgccgc cggacgtgct
    29641 ccgacggcga taaaggcatc aatgtgcctt gatatacaac tctttgctgt agacttgatc
    29701 ggcatagagg gcagcacaag gttgcgccca tgccacacca ctcggcatgg gggtccgctc
    29761 cgggcgattc gagaagttga ggttatgcga gtccagggaa gcctaaagca tcaaagtagt
    29821 cgactgtaag tatacggttc agcccagttt agctgtgtct ccaccagtgc cccgcgcctc
    29881 ctgccataag tgcggcttgc tcatggatat ccccgagacg gcttacggcg caccggataa
    29941 taaatttcgc gtatacatgg tattccttac cggagaaatt ataagcccaa caacaatacg
    30001 cctgtgacat tgttcgctca aacttatcgc gagtgatttg gctcacactc cggtaaagat
    30061 tccccatcac gccaacggca cataaacatc cgtttccagt cgctgctctg tcaccccttc
    30121 ggcaagattg gtgtagtgaa acagaatcgg tgctttacgc attttctcgc cgcttgcagg
    30181 cagccagtgg cgaataatgc cccatatcgt gtgtgaaata tcgtccagct cgccaacgtg
    30241 gcgggccacg gcataacgtc caccggtaag ttcaccattg ctcacaccat aacgattatc
    30301 gggaatcagt tcgctaacgc tgccgcagat atcaaagcga aacgcttccg gtgcggtggt
    30361 ggcaggggcg tcccaggcga cgccaaaagt ctggctttgg ttaacaggag aaagccccgt
    30421 ctctttacgc cacatgataa atttcgccac actgtagttg agcaactcag ggctacagcg
    30481 gtgcggcaac atcgccacag ggattgatgg gaaatcgata atgtttacat ccagtgtcag
    30541 gtttgtcatt ttgcctccct gcgaaattat tcccgggaac gcgtcccggg agcggtaaca
    30601 attacggatt tactttgcgg atctgtttga cgtcaatttc aacagaattc cagtctttat
    30661 cgacttcacc ctgaatctca accgtatctt tcggcgtcac cgtcacaccg ttccagcgtt
    30721 tgtgatcgat atcaacattg atagtaccgc tggcatcttt aaacacgtag agatcgtcag
    30781 agatgcgttc aacgatattg ccgcgcaggg ttacccaggt gtcgtcacgc agggattttg
    30841 cgctttctac agtcgttacg ctgccgttcg gcccctgaaa tcctccggcc tgactttgcg
    30901 ttaccgatgg gccagaaaaa ccgccctgct ctgctgccat caccggtgca ctgcacaggg
    30961 ccattactgc gattactgct gcgaattttt tcatgtttat tactcccttt aatgtctgtt
    31021 tccgagcatt taacaagata gtccttaaca acttcttaag ggaaaaaaat aaaatttaat
    31081 gctgtacaga gcgcgttaca acacggttta ctggcagcaa atacggttat cgcagggatg
    31141 aaaaaatgcg aattttactg atagaagatg acatgctgat tggtgacggc atcaaaactg
    31201 gccttagtaa aatgggtttt agtgtcgact ggtttacaca aggtcgtcag ggaaaagagg
    31261 cgctttatag cgcaccttat gatgcggtga tcctggattt aaccttacca ggcatggatg
    31321 gtcgcgatat tttgcgcgaa tggcgagaga aaggtcagcg tgagccggta ctgaacctga
    31381 ccgcgcgtga tgcgttagcg gaacgtgtag aagggctgcg tctgggagct gacgattatc
    31441 tgtgtaaacc ttttgcgttg atagaagtcg ccgccaggct ggaggctctg atgcgccgaa
    31501 ccaacggcca ggccagcaac gagctgcgcc acggtaacgt catgctcgac cccggcaaac
    31561 gtatcgccac gctggctggc gaacccttaa cactgaaacc aaaagaattt gccctgctgg
    31621 aattactgat gcgtaacgct ggtcgggtac tgccgcgcaa actgattgaa gagaaactgt
    31681 atacctggga cgaagaggtc accagtaatg ccgttgaagt gcatgtgcat catctgcgac
    31741 gcaaactcgg tagtgatttt attcgtaccg tgcatggtat tggctacaca ttaggtgaga
    31801 aatgaaattt acccaacgtc ttagtctgcg cgtcaggctg acgctaatct ttttaattct
    31861 ggcctcggtg acctggctgc tttccagctt tgtcgcctgg aaacaaacaa cggataacgt
    31921 cgatgaattg ttcgacaccc aactgatgct gtttgccaaa cggttaagta cgctcgatct
    31981 caacgaaatc aacgcggcgg atcgcatggc acagacgcca aataaattaa aacacggtca
    32041 tgttgatgac gatgcgctga cctttgccat ctttacccac gacggcagaa tggtccttaa
    32101 tgatggcgat aacggagaag atattcccta tagctatcaa cgggaaggtt ttgctgacgg
    32161 gcaactggtc ggtgaagacg atccttggcg ttttgtctgg atgacctcac ctgatggcaa
    32221 atatcgcatc gttgttggcc aggaatggga ataccgtgaa gacatggcgc tggcgattgt
    32281 tgccgggcaa ttgatcccgt ggctggtcgc actgccgatt atgttaatca tcatgatggt
    32341 actactgggt cgtgaactcg cgccgctgaa caaactggcg ctggcactac gtatgcgtga
    32401 ccctgactcg gaaaaaccac taaacgcgac tggcgtaccc agcgaagtgc ggccactggt
    32461 tgagtcgcta aatcaactgt tcgcccgcac acatgcgatg atggttcgag aacgacgctt
    32521 tacctccgat gcagctcacg aactacgtag cccgttaaca gcgctgaaag tgcaaaccga
    32581 agttgcgcag ctctctgacg atgatccgca ggcgcggaaa aaagcactgc tccaattaca
    32641 ttccgggatc gatcgcgcta ctcgtctggt tgatcaactg ctcacgctat cgcggctgga
    32701 ctcactggat aaccttcagg acgtcgcgga gatcccgctt gaagatctcc tacaatcgtc
    32761 ggtgatggat atttaccaca cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa
    32821 tgcccacggc atcaaacgca ccggacaacc gctattgcta agtttgttgg tgcgaaattt
    32881 gctggataac ggccccggtg tgacaccaga ggcactggcg cgaattggcg aacgcttcta
    32941 tcgcccaccc ggacaaaccg ctaccggcag tggacttggg ctatcgattg tccagcgaat
    33001 cgccaagctg catgacatga atgttgagtt tgggaatgcg gaacaaggtg gatttgaggc
    33061 gaaggtaagc tggtaacttc tctgtctatg agagccgtta aaacgactct catagatttt
    33121 actaatagca aaatataaac cgtccccaaa aaagccacca accacaaacc agacaaataa
    33181 cgagcgagaa tacatatagc caaaactcga acatttcccg cataaagagt tttcttaaga
    33241 taagaataat aagtggcgta agaagaaaaa atgctgcaat cattgcttgc aatatacgat
    33301 cttcgcctga aacggaaggt atactgcaaa tattgcctcc cgactcaagc atccagtcat
    33361 acttattgaa agcaaaaaaa gaatctatta accaataagt aagcattata tatagcaatg
    33421 cttcaaatat tgttttaatt ttctgcttta acatatatca gtacgctcat aaaaatccat
    33481 ttataattat caacatgaca cgttgatatt ttcatctttt acttattcaa agccatattt
    33541 tcaatcccca aaaaaaccga accaaacgtg taaattattc cggtgcgagt aagaattatg
    33601 aggaatggct atcagtattg tcattttcag aaaatattta tcctgcatcg gtgagtcaga
    33661 gctagatcag acttttgcta aattcgcaaa agactttgca cattttgcta atttcacagt
    33721 accgctctgt gacgtactat agtcgacaaa cgtctcacct tgagattaaa aaatgagcaa
    33781 catcctgatt atcaacggcg cgaaaaaatt cgcccactcc aatggtcaac tgaacgacac
    33841 cctgaccgaa gtcgcggatg gcacactgcg cgacctcggg catgatgtcc gcatcgttcg
    33901 cgccgacagc gactacaatg tcaaagcgga agtacaaaac tttctctggg ctgatgtggt
    33961 gatctggcag atgccaggct ggtggatggg cgcgccgtgg acagtgaaaa aatatattga
    34021 tgatgtattc accgaaggtc acgggactct gtatgccagc gatggtcgta cccgcaaaga
    34081 tccgtcgaaa aaatacggtt ccggcagcct ggtacaggac aaaaaatata tgctttctct
    34141 aacctggaac gcaccaatgg aagccttcac cgaaaaagat cagttcttcc acggcgttgg
    34201 cgttgacggt gtgtatctgc cgttccataa agcaaaccaa ttcctcggta tggaaccgct
    34261 gccgacattt atcgctaatg acgtgataaa aatgcctgat gttccccgct atactgaaga
    34321 atatcgcaag catcttgtgg aaatttttgg ttaactagag ctcaggcttt agaaggagtt
    34381 aaccatgctt accgtaatcg cagaaatccg tactcgtcct ggtcaacatc accgtcaggc
    34441 ggtattggat cagtttgcta aaatcgttcc aaccgtactg aaagaagaag gttgccacgg
    34501 ctatgcgcca atggtggatt gcgctgctgg cgtgagtttc cagtctatgg caccggattc
    34561 tatcgtgatg attgagcagt gggaaagcat cgcgcacctt gaagcgcatc tgcaaacccc
    34621 gcacatgaag gcgtacagcg aagccgtaaa aggcgacgtg ctggagatga atatccgtat
    34681 tctgcagcca gggatttaat cctgccttgt ttgcccggcc atcctgaccg ggcaatgttc
    34741 tttcctttaa acctcaatct ccgccatgtc gcctttctct tgcaaccagt tgcggcgatc
    34801 ttccgagcgt ttcttcgcca gcagcatatc catcatcgcg tcagtacgct gatcgtcttc
    34861 atcatcgata gtcaactgca ccagacggcg agtgttcgga tcaagcgtgg tttcacgcaa
    34921 ttgcatcggg ttcatttccc ccagaccttt aaaacgctgg acgttcggct tgcctttctt
    34981 gcgttttaat tgctcaagta cgccctcttt ctcttcttcc gtcagcgcgt aataaacctc
    35041 tttcccgaga tcaatacggt agagcggtgg cagtgcgacg taaacgtgac cgtgtttcac
    35101 caacgcgcgg aagtgtttta cgaacaaagc gcagagcagc gtggcaatgt gcagaccatc
    35161 agagtccgca tccgcgagga tacagatttt gccataacga agctggctca gatcgtcgct
    35221 gtcaggatcg ataccgatcg ctaccgaaat atcatgcact tcctgcgagg ccagcacttc
    35281 gtcggaagaa acttcccagg tgttaaggat cttacctttc agcggcatga tcgcctgata
    35341 ttcacgatcg cgcgcctgct tggcagatcc gcctgcggag tcaccttcca cgaggaacag
    35401 ttcggtacgg ttaaggccct gcgcggtaca atcagccagt ttgccaggca gcgccgggcc
    35461 gctggtcagc tttttacgca ccactttttt agccgcacgc atacgacgct gggcgctgga
    35521 aatcgccatc tctgccagca actccgccgc ctgaacgttc tggttcagcc acaggatgaa
    35581 ggcatctttc accacgcccg aaacgaatgc cgcacactga cgcgaagaga gacgctcttt
    35641 ggtctgcccg gcaaactgcg gatcctgcat tttaaccgac agaacatagg cgcagcgatc
    35701 ccagatatct tccgccgaca gcttcacgcc gcgcggcaga atgttgcggt attcacagaa
    35761 ctcacgcatc gcgtccaaca ggccctgacg cagaccatta acatgggtac cgccctgcat
    35821 cgttgggata aggttgacgt agctttcggt cagcagttca ccgccttccg gcagccacag
    35881 tagcgcccag tccacagctt cagtatcgcc agcgaaatta ccgataaacg gtttttctgg
    35941 cagcgtcggt aaaccgttta ccgcttccgc caggtaatca ttcagaccgt cctgatagca
    36001 ccagcgctgt tcggtgttgt tgatctcatc tttaaaggta atctcaacgc cggggcacag
    36061 tactgctttg gccttcagca catgcgtcag gcgtgaaaca gaaaaacgcg ggctgtcaaa
    36121 gaaggtttca tccggccaga agtgcacgct ggtaccggta ttgcgtttac cgcaagtgcc
    36181 gacaacctgt aaatcctgca ccttttcgcc attttcaaag gcgatgttat aaacctgacc
    36241 atcgcggcgc acgttaactt ctacgcgctt cgacaaggcg ttaactaccg aaatccccac
    36301 gccatgcagg ccgccagaga actggtaatt tttgttagag aatttaccgc ctgcatgcag
    36361 acggcaaaga atcagttcaa ccgccggtac tccttcttcc gggtgaatat ccaccggcat
    36421 cccgcgccca tcgtcaatca cttctaacga ctggtcagca tgtaagatta catccacgcg
    36481 ttttgcgtga cccgccagtg cttcatcgac actgttatca ataacttctt gccccaaatg
    36541 gttagggcga gtggtatcgg tatacatccc cggacggcgg cgaaccggct caagcccggt
    36601 gagtacctca atggcatcag cgttataagt ttgcgtcatg gtttaagtta gcaattcgag
    36661 ttgatcgtca gagatggtgc agaccaagaa aatcgacgat cgggttgaaa taatcttcga
    36721 agcccgtgaa tgcgtggttg ccgccttcta tgacagtctg gcggcaggaa gcatagtacg
    36781 ccaccgcctg gcggtaatcc agcacttcat ctcccgtctg ttgcagcagc cagatcaaat
    36841 ccggcgcttc cagcgggtca atctgcatga ctttgagatc gtaaatatgg cgtgactcta
    36901 gcacatattg ctgcccggtg taggggttct cgttctgacc gagatagtcc gtcagcaatt
    36961 caaacgggcg taccgccggg tttaccacca ctgcgggcag cataaaacat tgcgacaacc
    37021 aggtggcgta atatcccccc agtgacgaac cgacaatacc cagcgaatcg ccgccatgtt
    37081 ccaggacaat ggattccagc agttctgccg cgtcggaagg atacggcggc aactgcggaa
    37141 tgatcatctc aacgtcaggg tgatgttccg ccagccagtt ttttaacaag ctcgcttttg
    37201 cagagcgcgg cgagctgttg aaaccgtgta aataaagaag cgtagacatc agtagccttc
    37261 tgaagcggta tcaggttgga aacgtgtgtc cgccaggcga tgcacctcgg tggtcagcgt
    37321 gccatcagca tgtaactcga gagtacgcca gccgggcgcg atagtatcca gcgtaaagtt
    37381 ggaacagtgc ggcttaaact gcacacaggt cgacggcgtt gccagcaggc ggcgaccgtt
    37441 ccagtcgaga tccagctcct gatgaatatg accgcacagc aagtatttga cgtgcggaaa
    37501 cttcgccagc acggtatcca gttcgcccgc gttacgcaga ctgtgttgat cgagccaact
    37561 acaacccgca ggtagcggat gatgatgcag cagcagcaac gtatggcgtt ctggcgcatc
    37621 ggccagttta cgttccagcc actcaagctg aaactcgctc agctcaccgt gcggcacgcc
    37681 aaacacctgg ctatccagca acaggatttg ccattgctca ccaataaaca cccgcttcgc
    37741 cggggagata cccgcatcct gtaacgcgct gtacatcgcg ggctggaaat cgtggttgcc
    37801 cggcagccag acgcagggcg cacgaaaact tgcgatgcct tcagcgaaat gctgataggc
    37861 cgcagaggat tgatcctgcg ctaaatcacc tgtcgcgaca atcaggtcga attcgtgctg
    37921 gtgtggacga atcgcctcca gcaccgcctg gtagctttcc caggtgttta cccctaacag
    37981 ggcttcgtgc ttttgtgcaa acaggtgagt atcggtaatt tgtaaaatcc ttcctctggc
    38041 ctcaccagcc agaggaaggg ttaacaggct ttccaaatgg tgtccttagg tttcacgacg
    38101 ctagtaaacc ggaatcgcca tcgctccatg tgctaaacag tatcgcaacc agtccgctaa
    38161 aaactggtta atttgatgct tttcgtcgcg ttgatgcaac tttttattag gataatcata
    38221 ccgcgctttg aagcgaaaaa tctgctggct tgaacacact tcagccacca tcgcgtcatg
    38281 atacagacgc accgtcattg acggaaggct ccagtaactg atcgcgggcg cagtctgttc
    38341 tattgtcacc agggtagtgt atcgggtcga ttccacaatc gtcagccgat attgtgcgtt
    38401 tgccacctga tagcttacag tttcgccggg tgcgtcattg cgcggtaaca aacggcgcaa
    38461 ttgtgaaaag ttcatctcgc acaggcgcat catttcagga aagtcaggtg tgtaacgctt
    38521 catttatgcc cactcatttt ttaacgcttg atgatgcagc tgcagccatt gcaaagcgat
    38581 gaccgacgct gcgttgtcga ttttcccctc ttctacccac tggtatgcct gttcccggct
    38641 taccacatga acgcgaatat cttcgttttc atcagccaga ccatgaatac cgcttgcggt
    38701 cgtggcgtcc acttcgccca ccataattga cgaacgctca ctggtgcccc ccgggcttgc
    38761 caggaaactt aacaccggtt tggtccgttt gactatcagt cccgcctctt caatcgcttc
    38821 gcgacgggca acatcttcca cactttcacc ctcttcaatc atcccggcaa ccatctccag
    38881 tagccagggg gtttcgctgg tgtcgtacgc ggcaatccga atctgctcaa tcagcacaac
    38941 ttcatcacgc actgggtcaa agggtagcaa gactgcggcg tgaccgcgct caaaaatttc
    39001 ccgccgtacc tcatgactca tttgcccgtt gaatagacga tgacgaaatc tataaagatc
    39061 taatgaaaaa aagccgcgat aaagtgtttc tcgtgcaata atttctacat cgtttttgcc
    39121 aaatgtaacg ggcaggttgt ctggcttaag cattgttaat gtcctagcac taatagtgaa
    39181 ttaaatgtga atttcagcga cgtttgactg ccgtttgagc agtcatatgt taaattgagg
    39241 cacattaacg ccctatggca cgtaacgcca accttttgcg gtagcggctt ctgctagaat
    39301 ccgcaataat tttacagttt gatcgcgcta aatactgctt caccacaagg aatgcaaatg
    39361 aagaaattgc tccccattct tatcggcctg agcctttctg ggttcagttc gttgagccag
    39421 gccgagaacc tgatgcaagt ttatcagcaa gcacgcctta gtaacccgga attgcgtaag
    39481 tctgccgccg atcgtgatgc tgcctttgaa aaaattaatg aagcgcgcag tccattactg
    39541 ccacagctag gtttaggtgc agattacacc tatagcaacg gctaccgcga cgcgaacggc
    39601 atcaactcga acgcgaccag tgcgtccctg cagttaactc aatccatttt tgatatgtcg
    39661 aaatggcgtg cgttaacgct gcaggaaaaa gcagcaggga ttcaggacgt cacatatcag
    39721 accgatcagc aaaccttgat cctcaacacc gcgaccgctt atttcaacgt gttgaatgct
    39781 attgacgttc tttcctatac acaggcacaa aaagaagcga tctaccgtca attagatcaa
    39841 accacccaac gttttaacgt gggcctggta gcgatcaccg acgtgcagaa cgcccgcgcg
    39901 cagtacgata ccgtgctggc gaacgaagtg accgcacgta ataaccttga taacgcggta
    39961 gagcagctgc gccagatcac cggtaactac tatccggaac tggcggcgct gaatgtcgaa
    40021 aactttaaaa ccgacaaacc acagccggtt aacgcgctgc tgaaagaagc cgaaaaacgc
    40081 aacctgtcgc tgttacaggc acgcttgagc caggacctgg agcgcgagca aattcgccag
    40141 gcgcaggatg gtcacttacc gactctggat ttaacggctt ctaccgggat ttctgacacc
    40201 tcttatagcg gttcgaaaac ccgtggtgcc gctggtaccc agtatgacga tagcaatatg
    40261 ggccagaaca aagttggcct gagcttctcg ctgccgattt atcagggcgg aatggttaac
    40321 tcgcaggtga aacaggcaca gtacaacttt gttggtgcca gtgagcaact ggaaagcgca
    40381 catcgtagcg tcgtgcaaac cgtacgttcc tccttcaaca acattaatgc ttctatcagt
    40441 agtattaacg cctacaaaca agccgtagtt tccgcgcaaa gctcattaga cgcgatggaa
    40501 gcgggctact cggtcggtac gcgtaccatt gttgatgtgt tggatgcgac caccacgctg
    40561 tacaacgcca agcaagagct ggcgaatgcg cgttataact acctgattaa tcagctgaat
    40621 attaagtcag ccctgggtac gttgaacgag caggatttgc tggcactgaa caatgcgctg
    40681 agcaaaccgg tttccactaa tccggaaaac gttgccccgc aaacgccgga acagaatgct
    40741 attgctgatg gttatgcgcc tgatagcccg gcacccgtcg ttcagcaaac atccgcacgc
    40801 actaccacca gtaacggtca taaccctttc cgtaactgat gacgacgacg gggcttcggc
    40861 cccgtctgaa cgtaaggcaa cgtaaagata cgggttatct gccgcattct tcccccttct
    40921 cgcttcaatt tcgaccagcc atcctctatt ctgatgggta tttaccactg gtcccggaag
    40981 acaaaaatga aacggacaaa atccatacgc cacgcatcgt tccgcaaaaa ctggagcgca
    41041 cgccatctga caccagtcgc tctcgcggtt gccactgttt ttatgctggc tggctgtgaa
    41101 aagagtgatg aaacagtgtc tctctatcaa aatgctgacg actgttcagc tgcaaaccca
    41161 ggcaaaagcg ccgaatgtac caccgcgtac aacaatgcgc tgaaagaagc cgaacgtact
    41221 gcgccgaaat acgccacccg tgaagactgt gttgctgaat ttggtgaagg tcagtgtcag
    41281 caagcaccag cccaggctgg catggcacca gaaaaccagg cgcaggctca gcaatccagc
    41341 gggagtttct ggatgccgct gatggccggt tacatgatgg ggcgtctgat gggcggcggc
    41401 gcgggatttg cacagcagcc gctgttctcc tcgaaaaacc cggccagtcc ggcttacggt
    41461 aaatataccg acgcgacggg taaaaactat ggcgcagccc agccaggccg caccatgacc
    41521 gtaccgaaga cggcaatggc accaaaaccg gcgaccacca ctaccgttac ccgtggcggt
    41581 tttggtgaat ctgttgccaa acaaagcact atgcagcgta gcgcaaccgg cacctcttct
    41641 cgttcaatgg gtggctgata ccgatggaaa gagtcagtat taccgagcgc ccggactggc
    41701 gtgagaaagc ccacgaatac ggtttcaatt ttcacaccat gtacggcgag ccgtactggt
    41761 gtgaagatgc ttactacaag ttgaccctcg cccaggttga aaagctggaa gaagtcaccg
    41821 ccgaactgca ccagatgtgc ctgaaagtgg tggaaaaagt gatcgccagc gatgagctga
    41881 tgaccaaatt ccgcatacca aaacacacct ggagttttgt gcgtcaatca tggctgactc
    41941 accagccatc gctttattcg cgtcttgatc tggcgtggga tggcactggc gaacctaaac
    42001 ttctggaaaa taacgccgat acgccaacgt cactatacga ggcggcgttc tttcagtgga
    42061 tctggctgga agatcagctt aacgccggta acttgccgga gggcagcgac cagtttaaca
    42121 gtctgcaaga aaaactgatc gatcgcttcg ttgagctgcg tgaacagtat ggcttccagt
    42181 tgctgcatct cacctgctgt cgcgacacgg tggaagatcg cggaaccatt cagtatttgc
    42241 aggactgcgc aaccgaagct gaaatcgcca ccgagttcct ctacatcgac gagataggtt
    42301 taggcgaaaa aggccagttc acggatttac aggatcaggt gatttctaac ctgttcaagc
    42361 tttatccgtg ggaatttatg ctgcgcgaaa tgttctccac caagctggaa gatgctggcg
    42421 tacgctggct ggaaccggcg tggaagagca ttatctccaa caaggcactt ctaccgctac
    42481 tgtgggagat gttcccgaat cacccgaacc tgctgcccgc ttattttgcg gaagatgatc
    42541 atccgcaaat ggaaaaatat gtggttaaac cgatcttctc ccgtgaaggc gcaaacgtgt
    42601 cgatcattga gaacggcaaa accattgaag cagcggaagg tccgtatggc gaagaaggga
    42661 tgattgttca gcaattccac ccgttaccga aattcggcga cagctatatg ctgattggta
    42721 gctggctggt gaacgatcaa cctgccggaa ttggcattcg tgaagaccgt gcgttgatca
    42781 cccaggatat gtctcggttt tatccacata tttttgttga ataagccacg ataccggatg
    42841 gcactcgcca tccggtaatt gttagcctat ctgcaccgac agcatactta aactgcccat
    42901 ttctatacca tcgaccggaa tggtaattgg ctcctgccca tcccacacac ctaacacata
    42961 caacaacggc agatagtgct ctggcgttgg gttcaataac gcgccacctt catggtcgag
    43021 ataattcacc agaggatgct gctctaccgg cccttgccac gtcagattcg ctttcacata
    43081 ctcattaaac gacgtcgccc acggatacgg tgaactatca ccgtgccact tcactgtgcg
    43141 caggttatgc accacgttac cgctggcgac caacattatt ccttcatcac gcagcgccgc
    43201 gagtttgcgc cccatttcga aatgccaggc ggcaggtttg ctactgtcga tactcaactg
    43261 caccataggg atatcggcgt caggatacat cttaatcagc acgccccacg agccgtggtc
    43321 aaagccccag gcttctttat ccagcgtcac cgggatcggc gctaacagct caaccagacg
    43381 ctgtgccagc gcaggcgaac ccggagcagg ataatgcgta tcgtacagcg cctgcgggaa
    43441 gccaccaaag tcatgaatcg tgggcggcgt ctccatcgcg gtcactcctg ttccacgggt
    43501 aaaccagtga gccgaaacca ccacaatcgc ttgcgggcgt ggcaatgtca tccccaactt
    43561 ctgccagctg cgggtataca aattatcttc cagcacgttc atcggactac cgtgacctaa
    43621 aaacaatgct ggcatacgtg ttgaagacat gatgatatcc ttaactaaag gtgtcatttt
    43681 gatatcctca caatacgctt gttcggcgga gtaagaaccc ggataacaat gatgatgatc
    43741 atcagttatt ttgacgatct gcctgaaggt gaagatttat aaggagttgt cgatgtcagt
    43801 acctctcatt ctgaccatac tggcgggggc agccacgttt attggcgcgt ttctcggcgt
    43861 tctcggacaa aaaccctcga accgcttact ggcgttttcg ctaggttttg cggcggggat
    43921 catgttgctc atctcattaa tggaaatgct tcctgccgca ctagcagctg aaggaatgtc
    43981 gccggtgttg ggttatggaa tgtttatctt cggtctgctt ggctactttg gcctggaccg
    44041 catgttgccg catgcgcatc cgcaggattt aatgcaaaaa tcggtgcagc tgttgccaaa
    44101 atcgatcaag cgcacagcca ttctgctcac tctcggcatc agtctgcata acttcccgga
    44161 agggattgcc acctttgtca cggcgagcag caacctggag ctgggatttg gcatcgcact
    44221 ggccgtcgcg ttgcacaata tccctgaagg tctggcagtg gcaggcccgg tttatgcggc
    44281 aacggggtct aaacgcaccg cgattctgtg ggcggggatt tctggactag cagaaattct
    44341 tggtggtgtg ctggcgtggt taatcctcgg tagcatgatt tccccggtgg tcatggcggc
    44401 aatcatggcg gcggttgcag gaattatggt ggcgctctcg gttgatgaat taatgccgct
    44461 cgccaaagag attgacccta ataataaccc cagctatggc gtactatgtg gaatgtcagt
    44521 gatgggattc agtttagtgc tgctacaaac ggcgggaatt ggttaaaaac aacatcagaa
    44581 gttgtccagc acatggacaa cttctgccag cttcacgaaa gccttattct accttttcgg
    44641 ctgtctcttc tctcgtactg tttaagtatt tgttccgcgc ttcaagcagt gcgtctctgt
    44701 cctggcgaaa cgcccgacaa taacgtttca aatgcaggaa ataacccacc acaaccagag
    44761 caatcaatac aatccagtac caggcgataa acatcctttt acccttaata tttaacaata
    44821 ctaaacattt agcgcataaa tttcacatat cctttttcgg atatattcat atggtcgcat
    44881 aataacggaa caattatatg attttgatca taatgaaaaa gaatgcctga tattaatgaa
    44941 attttctcat agaaagagaa gcggaattaa catcttgcat agcagggcaa aataaaaacc
    45001 gaagccagct acaacttcgg tttcttgatt aaggcagtaa ttaagcagcg gttttcagct
    45061 ggctttacgc tcatgtgcct gacggtatgc caccaggtct tcaatagtca cgagcgccat
    45121 attgtgttta ttggcaaact caatgcactc tggtgcacgc gccatcgtgc catcgtcatt
    45181 agtcagctca cacagcacac cagccggttt aaagcctgcc agcgtcatca aatcaatagt
    45241 cgcttcagta tgaccgccac gcgtcagtac accacctgcc tgcgcgcgaa gtgggaaaac
    45301 gtggcccggg cgattcagat cagaaggttt tgcgccatcg gcaatcgctg cgcgaacggt
    45361 cgtaatacga tcagcggcag aaacaccggt agtcacacct tcagccgctt caatggtcac
    45421 ggtaaaaccg gtgccatagg cgctggtgtt attttctacc atcattggca gatcaagttg
    45481 tttacggcga tcttcagtga tgcacaggca aacaataccg ctaccgtggc gaatggtcag
    45541 cgccatctgc tcaacggtca tggtttctgc cgggaagatc atatcgcctt cgttttcacg
    45601 gtcttcatca tcaagcacca ttacaccgcg tccttcacgc agcgcagcca gtgcattttc
    45661 aacacgttcg aaaggcgtac caaaagagga aagtagcgtc tgattcatgg taaaaaaacc
    45721 tcactaaaat tatggttacc agaatcaggg cagtcttagg agtggcggca tatagccaaa
    45781 ataacgtgag cgggtccatg cccgacagaa tcgttactct ctcccatccg gactctaacc
    45841 gtcggccccg gaattacacc ggatctgctg tcctttgagt tcgcacccaa agcgctcgcg
    45901 ggctttcaac tgagttgatt taccgccggt ggggaatttc gccccgccct gagaataagc
    45961 ggattcacta taacgctaat gattagcggc agcaacgcat agcttcacat aattctggtt
    46021 tatgacttac ccttatcgca ctacaatggt actcaacacc tatcagtaca gggaaactac
    46081 aatgattgac ccgaaaaaaa ttgagcaaat cgctcgccag gttcacgaat caatgcctaa
    46141 aggaatcagg gagttcgggg aagatgtgga gaaaaaaatc cgccaaaccc tacaagctca
    46201 gctgacgcgc ctcgaccttg taagccgtga agagttcgac gtccaaacgc aagtcctgtt
    46261 acggactcgt gagaaactgg cgctgcttga acaaagaatc agcgaactgg aaaatcgcag
    46321 tactgagatc aaaaagcagc cagatccaga gactctcccc ccaacgctgt aaacattt
//
LOCUS       DAZSNO010000017        44602 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZSNO010000017 DAZSNO010000000
VERSION     DAZSNO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44602
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.017"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(27..914)
                     /gene="rpnA"
                     /locus_tag="ACHWN2_000893"
     CDS             complement(27..914)
                     /gene="rpnA"
                     /locus_tag="ACHWN2_000893"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFR8930534.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
     gene            complement(1117..1353)
                     /gene="feoC"
                     /locus_tag="ACHWN2_000894"
     CDS             complement(1117..1353)
                     /gene="feoC"
                     /locus_tag="ACHWN2_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR8930535.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1353..3674)
                     /gene="feoB"
                     /locus_tag="ACHWN2_000895"
     CDS             complement(1353..3674)
                     /gene="feoB"
                     /locus_tag="ACHWN2_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR8930536.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3691..3918)
                     /gene="feoA"
                     /locus_tag="ACHWN2_000896"
     CDS             complement(3691..3918)
                     /gene="feoA"
                     /locus_tag="ACHWN2_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR8930537.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4356..6677)
                     /locus_tag="ACHWN2_000897"
     CDS             complement(4356..6677)
                     /locus_tag="ACHWN2_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR8930538.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6774..7250)
                     /gene="greB"
                     /locus_tag="ACHWN2_000898"
     CDS             complement(6774..7250)
                     /gene="greB"
                     /locus_tag="ACHWN2_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR8930539.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            7478..8197
                     /gene="ompR"
                     /locus_tag="ACHWN2_000899"
     CDS             7478..8197
                     /gene="ompR"
                     /locus_tag="ACHWN2_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR8930540.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            8194..9546
                     /gene="envZ"
                     /locus_tag="ACHWN2_000900"
     CDS             8194..9546
                     /gene="envZ"
                     /locus_tag="ACHWN2_000900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR8930541.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9622..11244)
                     /gene="pckA"
                     /locus_tag="ACHWN2_000901"
     CDS             complement(9622..11244)
                     /gene="pckA"
                     /locus_tag="ACHWN2_000901"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR8930542.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11623..13374
                     /locus_tag="ACHWN2_000902"
     CDS             11623..13374
                     /locus_tag="ACHWN2_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFR8930543.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            complement(13518..14387)
                     /gene="hslO"
                     /locus_tag="ACHWN2_000903"
     CDS             complement(13518..14387)
                     /gene="hslO"
                     /locus_tag="ACHWN2_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR8930544.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(14412..14813)
                     /gene="hslR"
                     /locus_tag="ACHWN2_000904"
     CDS             complement(14412..14813)
                     /gene="hslR"
                     /locus_tag="ACHWN2_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR8930545.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14824..15492)
                     /gene="yrfG"
                     /locus_tag="ACHWN2_000905"
     CDS             complement(14824..15492)
                     /gene="yrfG"
                     /locus_tag="ACHWN2_000905"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR8930546.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15557..17692)
                     /gene="igaA"
                     /locus_tag="ACHWN2_000906"
     CDS             complement(15557..17692)
                     /gene="igaA"
                     /locus_tag="ACHWN2_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR8930547.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            18012..18572
                     /gene="nudE"
                     /locus_tag="ACHWN2_000907"
     CDS             18012..18572
                     /gene="nudE"
                     /locus_tag="ACHWN2_000907"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR8930548.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18737..21289)
                     /gene="mrcA"
                     /locus_tag="ACHWN2_000908"
     CDS             complement(18737..21289)
                     /gene="mrcA"
                     /locus_tag="ACHWN2_000908"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR8930549.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21409..22188
                     /gene="hofM"
                     /locus_tag="ACHWN2_000909"
     CDS             21409..22188
                     /gene="hofM"
                     /locus_tag="ACHWN2_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR8930550.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            22188..22727
                     /gene="hofN"
                     /locus_tag="ACHWN2_000910"
     CDS             22188..22727
                     /gene="hofN"
                     /locus_tag="ACHWN2_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR8930551.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22711..23151
                     /gene="hofO"
                     /locus_tag="ACHWN2_000911"
     CDS             22711..23151
                     /gene="hofO"
                     /locus_tag="ACHWN2_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR8930552.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            23141..23547
                     /gene="hofP"
                     /locus_tag="ACHWN2_000912"
                     /pseudo
     CDS             23141..23547
                     /gene="hofP"
                     /locus_tag="ACHWN2_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            23459..24697
                     /gene="hofQ"
                     /locus_tag="ACHWN2_000913"
     CDS             23459..24697
                     /gene="hofQ"
                     /locus_tag="ACHWN2_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR8930553.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            25098..25619
                     /gene="aroK"
                     /locus_tag="ACHWN2_000914"
     CDS             25098..25619
                     /gene="aroK"
                     /locus_tag="ACHWN2_000914"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR8930554.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25676..26764
                     /gene="aroB"
                     /locus_tag="ACHWN2_000915"
     CDS             25676..26764
                     /gene="aroB"
                     /locus_tag="ACHWN2_000915"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR8930555.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26856..28148
                     /gene="damX"
                     /locus_tag="ACHWN2_000916"
     CDS             26856..28148
                     /gene="damX"
                     /locus_tag="ACHWN2_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR8930556.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            28255..29091
                     /gene="dam"
                     /locus_tag="ACHWN2_000917"
     CDS             28255..29091
                     /gene="dam"
                     /locus_tag="ACHWN2_000917"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR8930557.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            29109..29786
                     /gene="rpe"
                     /locus_tag="ACHWN2_000918"
     CDS             29109..29786
                     /gene="rpe"
                     /locus_tag="ACHWN2_000918"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR8930558.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29779..30537
                     /gene="gph"
                     /locus_tag="ACHWN2_000919"
     CDS             29779..30537
                     /gene="gph"
                     /locus_tag="ACHWN2_000919"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR8930559.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30530..31534
                     /gene="trpS"
                     /locus_tag="ACHWN2_000920"
     CDS             30530..31534
                     /gene="trpS"
                     /locus_tag="ACHWN2_000920"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR8930560.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31804..32709
                     /gene="yhfZ"
                     /locus_tag="ACHWN2_000921"
     CDS             31804..32709
                     /gene="yhfZ"
                     /locus_tag="ACHWN2_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR8930561.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32726..33088
                     /locus_tag="ACHWN2_000922"
     CDS             32726..33088
                     /locus_tag="ACHWN2_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR8930562.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            33172..34335
                     /locus_tag="ACHWN2_000923"
     CDS             33172..34335
                     /locus_tag="ACHWN2_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFR8930563.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            34336..35562
                     /locus_tag="ACHWN2_000924"
     CDS             34336..35562
                     /locus_tag="ACHWN2_000924"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR8930564.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            35559..36437
                     /locus_tag="ACHWN2_000925"
     CDS             35559..36437
                     /locus_tag="ACHWN2_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR8930565.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            36448..36801
                     /locus_tag="ACHWN2_000926"
     CDS             36448..36801
                     /locus_tag="ACHWN2_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR8930566.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36813..38117
                     /locus_tag="ACHWN2_000927"
     CDS             36813..38117
                     /locus_tag="ACHWN2_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR8930567.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            38129..39214
                     /locus_tag="ACHWN2_000928"
     CDS             38129..39214
                     /locus_tag="ACHWN2_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR8930568.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(39374..40105)
                     /gene="frlR"
                     /locus_tag="ACHWN2_000929"
                     /gene_synonym="yhfR"
     CDS             complement(39374..40105)
                     /gene="frlR"
                     /locus_tag="ACHWN2_000929"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR8930569.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(40205..40990)
                     /gene="frlD"
                     /locus_tag="ACHWN2_000930"
                     /pseudo
     CDS             complement(40205..40990)
                     /gene="frlD"
                     /locus_tag="ACHWN2_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            complement(40987..41817)
                     /gene="frlC"
                     /locus_tag="ACHWN2_000931"
     CDS             complement(40987..41817)
                     /gene="frlC"
                     /locus_tag="ACHWN2_000931"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFR8930570.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(41867..42889)
                     /gene="frlB"
                     /locus_tag="ACHWN2_000932"
     CDS             complement(41867..42889)
                     /gene="frlB"
                     /locus_tag="ACHWN2_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR8930571.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42910..44247)
                     /gene="frlA"
                     /locus_tag="ACHWN2_000933"
                     /pseudo
     CDS             complement(42910..44247)
                     /gene="frlA"
                     /locus_tag="ACHWN2_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(44542..44601)
                     /locus_tag="ACHWN2_000934"
     CDS             complement(44542..44601)
                     /locus_tag="ACHWN2_000934"
                     /inference="COORDINATES: protein motif:HMM:NF025344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4223 family protein"
                     /protein_id="HFR8930572.1"
                     /translation="MHPAISISKIIGGCGPTAQ"
BASE COUNT        10387 a        11322 c        12210 g        10683 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tactcgccgc agccaactct
       61 tcttctgaca gcccggtaaa tcgcatgatg tctgcaagag ggactccgga ttcaagcatt
      121 attttggcta tatgcagggc tttggattgt tccccctcct gccgcaatct ttccgcaata
      181 gtcattaaac tctccttgtg tttcggtgaa cgttcggcaa cgccgtcgat aaaatcgtta
      241 aaacgtacag cgtcgccagt ttgcagtatg taattaaaca gccctttgat ttgtctgtca
      301 ttagcgtatc cactacttaa taagcaggcc atttgctcta ccagccccat cagatcgcgt
      361 tgacgaatat gtttttgaat taactccagc agcgccatac gtcggtgctg catgatttca
      421 tcatcaggca tgacggtgac atcaatcagc ggaaatgcgg aggcataaag ctgccttgcc
      481 agtttgggat cggcgaaaca atccagccaa cacagcgaat agggataggg gctttcaata
      541 ccgtggtaaa acaacaatgg caccaccatc ggcaacgttt tgtatccagc atcaaggtga
      601 ttttgcattg cggcaatagc gtaacgcatc atgcgaaatg cgatcagttt gtttgaggtg
      661 ctttgatgtt caatcagaca atagatgtat cctggtcctt gttccgtttt caccgaccac
      721 agcacatcgg aatagctttc acgcagatcg tcatcaataa agctcctcga ctccagcttg
      781 agggttttca tatcacagag cgcgtgaatg ggtgccggta aatgaaacgc aagaaaatcg
      841 cgtgccgtct ccggttggcg taaaaagagt ttgaacagcg catcatgtgg ggtggaactc
      901 tgctttttac tcatggcgtt ccttcacctg ttgtttgaat gccgcgactc tacgcgccac
      961 tccacatctg tgcattagcg agttttcatc cctgcgagac gctttcagag gaacttacgt
     1021 tgcgctgcaa gaattgcatt tttatggaaa gccgcacgca aaaaagcccg gatgcaaacg
     1081 cataccgggc ttttcaaaac aggcgatgga gtatggttaa cgcagcgccc accactcgcg
     1141 cagacaggct ttgccttccg ggcagctttt acaactgcca gagaggcagc cgtcaggttc
     1201 ttcctgaatc cgcacggctt tgcccatgct ttccagctgt tgcagcatgg cgttaatcat
     1261 tggctgcgga gtgttcaatg tctggcttat ctgggtcgct tccatacggc cccgtaacgc
     1321 cagcaaatca cgtacctgaa taagtgaagc cattaatggc aatcaccggt ggtgctggct
     1381 gcgcagcaac tgcttaccga cttgcgggtt gccagcagtt cgatatccac ccggctacgc
     1441 gcgcggcgca gcagaccgat agctacgatg ttaaacagga ttaccgccag aatgcacacc
     1501 aggctgtaag ttgggtgctg actgtagctg gcgacctgat agaacaatgt tgccagtgag
     1561 taagcgatat tcagccccca caggatggag aagcccatcc agccacggct tgattcacgg
     1621 gcgatggctc ccatcaccga gatacatggt acatacagca ggacgaaaat caggtagctg
     1681 tatgcggcag ctgcgctacc gaatttctga tccatcacgc ccatcgcccc ggtacccatt
     1741 tcaccgttgc ctttgctagc ttcaatgggg ttcatcagta cgctaaggct gaaggtgtct
     1801 ttcaggctct gccaggtttc atctaccgca ctgaacagct cttcaccgag gttgaattcc
     1861 gccggattga actcttcgtc ctgaatattt tctgcggtgt agagggtgtt gagcgtaccc
     1921 accaccactt cttttgccat tgcgcccgta aacaggccaa ccgttgcctg ccagttatct
     1981 tcatgtacgc caatcggctt gaagacaggg gttatcaccc ggctgacgga agccagcgcc
     2041 gagtcgttga tgttatcgac gattttcccg ctcaacgaga agctgttgaa agcgctcagg
     2101 aaaatgctga cgatgataat cactttacca gcacgcagaa cgaagccctt cagacgctgc
     2161 caggtctgga taatcaggct tttaacgtgt ggtacatggt agaccggcag ctccatgaca
     2221 aacggcgtcg cttcaccgcg catgatggtg tacttgagca tcaggccagt cagcaccgcc
     2281 atcacgatac cgagcatata cagcgagaaa accgccaggg caccgttctg cccgaagaag
     2341 gcagccgcga atactgcgaa gatagccaga cgcgcgccgc aggacataaa cggtgccatc
     2401 atgatggtca tcagacgttc acgcggtgca tcaagcgtac gtgcgcccat taccgacggt
     2461 acgttacaac cgaaaccgac gatcagcggc acaaaggatt tccccggcaa gcccagcgcc
     2521 tgcatcaggc ggtccatcac aaacgctgca cgcgccatat agccggagtc ctcaaggaag
     2581 gagaggaaca ggtacatcat gccgatctgc ggcaccagtg gcagcacggt gttaataccg
     2641 ccacccaggc cctgggcgag gaagatagtc agccagtccg ggaagtggag cgtgtagcca
     2701 atccattgaa taccatgcac aaatagcgcc acggagccga cgttaaacag cggctgtaac
     2761 gccccgccga tgttgatagc cagcaggaac atcaggtaca tcacaaagag gaaaatcggc
     2821 agaccgagga aacggttgag cacgatttta tctaccgcag tggtgaaacg gctgggttct
     2881 gccgtcaggg tgttgcttac cacatcacag atggcagcaa tgcactggta acgcgcatcg
     2941 gcaatgtgca gcgccggatc atccatttca ttacgcagac gggcgagggt ggcatccaga
     3001 tgctgcgacg cttcaccggc gtaggcgcgg ctgtagatat cgccttccag catttgcagg
     3061 cccagccagc gacgttgttt cagcgggatg tcggaaggca tcacttttgc cagtgaatct
     3121 gcttcgttga gtagcggctg tgcgtaatgc accagttcca cattctcgtt agctttatag
     3181 cgatcaatcg ccagcttgag cgcttcaata ccgcgaccac gggttgaaac cagcgggatc
     3241 accgggcagc caagacgtgc cgatagagca tcaatttcaa tacgaatatt ttgcttctcg
     3301 gcaatgtcga gcatgttcag cgccacgatg cagggaatgc cgagttccag cagttgcagc
     3361 gtcaggtaca ggttacgctc aaggttagac gcatccacca cgttaatcag caggtcggcg
     3421 tcgccactca aaatgtagtg acaggcgatt tgctcatcga gcgaggtctg cgatgagatg
     3481 gtggtcagag aataggtgcc gggcaggtcc accagcgtga cctgatgatc ggtggtggag
     3541 aattgccctt ctttacgttc gacggtaacg ccagcccagt tacctacacg ctgacgtgcg
     3601 ccagtgagct ggttaaataa ggttgtcttg ccagaatttg gattaccaat taagccaatg
     3661 gttaattttt tcattgttgt tatcaccgta ttaacaggaa accgcttcca cttctaataa
     3721 ggccagatct tttttgcgta ataccaggct cacacgacgg gtttcgatat gaatggggtc
     3781 gccgagtgga gcgacgcgca ccacatcaaa agaggagcca ggtaacatgc ctagagaaag
     3841 cagtttttgg cgatatgccg ggctgatttc acgggaaaag ccagtgattt tccacgcagt
     3901 atctggagtg tattgcatag gtgcctactt gtttctcatt aactggataa ctacctcatg
     3961 gggcgtttga ccgcgacatg agaccagatg atttgccaac aaaggagaga ttaaataatc
     4021 aacaacacaa tgataatgag aatggtttct atcagcaata cttaaaatgt gtgcgaatgt
     4081 tggtttgaat aataatctga ctcaatgttg atgtggctca aggttatatc gcagcgaaaa
     4141 tataattcga atagtgagtt acccggactg gctaatgttt aattaaggtt tcagttgtta
     4201 atgaagtgat aaatattaac ggagtatatt gtgagtttgg ctgtggaata aaagtgccgg
     4261 agatattctc cggcactttt atgtcaggca aagcgttcgt tgccagatgc ggcgtgaacg
     4321 ccttatcgcg caggtttagg ggccagtccg ggcgtttaac gttttttacc catcgctgcc
     4381 gccagcgcat ccatcatcgc gctattaccg gcaggctgcg cttcacgacc gcgtggtttg
     4441 gctgccgggc ggttgttttg cgggcgttca ttaccgccgc cgcgacgaga gttggtttcg
     4501 ccaggctgct cgtccagacg catagtcagg gcgatacgtt tacgctgaag atccacttcc
     4561 agcactttca ccttcacaat gtcgcccgct ttcaccacgg tatgcggatc ttccacaaac
     4621 ttgttcgaca atgaagagat gtgaaccagg ccgtcctgat gcacgccaat atcgacaaac
     4681 gcgccaaagt tggtgacgtt ggtcactgcg ccttcgagga tcatacccgg ttgcaggtcg
     4741 ttcattgtct cgacgccatc ggcaaactga gcggttttaa attccggacg cggatcgcga
     4801 cccggttttt ccagctcttt gatgatgtca gttactgtcg gcacaccgaa tttttcatca
     4861 gtaaagtcag acgctttcag gttacgcagt tcgctgctgt tacccatcag atctttcagt
     4921 gcctgctgtg ttgctgccag aatgcgttcc accaccggat aggcttccgg atgaacggta
     4981 gaggcgtcca gcgggttatc accgtggtta atgcgcagga agcccgcgca ctgctcgaag
     5041 gctttcggcc ccagacggct gactttcagc agttgctgac ggttctggaa ctggccgttc
     5101 tcatcgcgcc aggccacgat gttttgcgcc atcatgcgcg tcaggcccgc cacgcgggtt
     5161 aatagcggaa cagaagcggt gttgagatcg acgccaacgg cgtttacgca gtcttctact
     5221 actgcatcca gtttgcgggc cagttgcgtc tggctgacgt catgctgata ctgacctacg
     5281 ccgatagatt tcggatcgat tttcaccagc tccgccagcg gatcctgcaa acggcgggcg
     5341 atagataccg caccacgcag cgaaacgtcg agatccggga actcctgcgc tgccagctcg
     5401 gaagctgagt aaaccgacgc gccagcttcg ctgacgatca ctttctgtgc ggtcactttc
     5461 gggaactgct tctgcacgtc gagatagaaa cgttcagttt cgcgggaagc tgtaccgttg
     5521 ccgatcgcca ccagttcaac gttatgtttt tcacacaagg cagcaacggt catcgctgct
     5581 tttgcggctt gtccggtgtg cgggtaaatg gtatcggtcg ccaccagttt gccagtggca
     5641 tcgaccaccg ccacttttac cccggtacgc agacccggat cgaggcccat cgttgcacgc
     5701 agtccggcag gggccgccat cagcagatcg tgcaggttac gggcaaagac gttgattgct
     5761 tcatcttccg cgcgttcgcg cacggtaccc atcagttcgg tttccagatg catcagcacc
     5821 ttgatgcgcc aggtccagct cactacgctt ttgcgccagc tatccgccgg ggcattgttc
     5881 aggcgcaggc caaggtgatc catgatgatt tgctcgcaat agctctcttt gggcggctca
     5941 tcgaactgcg gatcggcatt cagcgaaagc tggagtacgc cttcgttacg cccacggaac
     6001 atcgccagcg cgcggtgaga aggcaccgtg gacaacggtt cgtgatgatc gaaatagtcg
     6061 cggaatttcg ccccttcctc ttctttaccg ctcaccaccg tagaaaccaa atgcgcgttc
     6121 ttccacagat aatcacgcac tttcgccagc agcgcggcat cttcggcaaa ccgttccatc
     6181 aggatatagc gcgcgccatc cagcgcggct ttggtatctg ccacgccttt atcggcatca
     6241 acatattgtg cagcggcgac ttctggcgtg tgtgacggat cgctccacag caggtcagcc
     6301 aacggctcaa gccctgcttc aatggcgatt tgcccgcggg tgcggcgttt aggtttgtag
     6361 ggcaggtaga ggtcttcgag ttcggttttg cttagggtgg cgttgatggc cttcgccaga
     6421 tcatcggtga gtttgccttg ctcggaaatg gatttgagga tcgcctgacg tctctcttcc
     6481 agctcgcgca gatagctcag acgcgtttcc agattacgca gctgcgtgtc atccagaccg
     6541 ccggtgattt ccttacgata acgtgcgata aacggcacgg tattcccttc gtcaagcagg
     6601 cgaacggcag cgtcaacctg ttccgggcgc gcctgaattt cacccgcaat aatgcggcag
     6661 aacgaatcat tcatcatggt ttgagttcat cttttcggat caaaaatcag gggatagtta
     6721 tacggactgg ctggcaaaaa tgccagccat cggcaggagg ttaagactct tccttacggt
     6781 ttcacgtact cgatagcatt aacataccag ttcgcttccc cggcaggggt attcaccacc
     6841 gccagatcgc cgacttcttt tttcagcaat gcgcgggcca tcggggaatc gatagagatg
     6901 taatctttac ggccaaaaat ttcatcgtag ccgacaatac ggaaacggtg agtcacgcca
     6961 tcgtcgtttt caatctccac ccacgcgcca aaaaagactt tgccttcctg ctgaggggag
     7021 taatcgacga ttttgagatt ttccaggcat ttagtgagat agcgcacgcg acggtcgatt
     7081 tcacgcagac gctttttatt atactgatag tcagcatttt cgctgcggtc gcccagactt
     7141 gcggcccagg tcaccttttt tgtgacctcc gggcgttctt cacgccagag ataattaagc
     7201 tcttgtttga gtttttcata cccttcccgg gtaaccaggg gcgttttcat ctcgttgatt
     7261 ccctttgtct gtttgataat gcgcacattg ggtataacgt gatcatatca acagaatcaa
     7321 taatgtttcg ccgaataaat tgtatactta agctgctgtt taatatgctt tgtaacaatt
     7381 taggctgaaa ttcataccag atttagctgg tgacgaacgt gagctttttt aagaatacac
     7441 gcttacaaat tgttgcgaac ctttgggagt acaaacaatg caagagaact acaagattct
     7501 ggtggtcgat gacgacatgc gcctgcgtgc gctgctggaa cgttatctca ccgaacaagg
     7561 cttccaagtt cgaagcgtcg ctaatgcaga acagatggat cgcctgctga ctcgtgaatc
     7621 tttccatctt atggtactgg atttaatgtt acctggtgaa gatggcttgt cgatttgccg
     7681 acgtcttcgt agtcagagca acccgatgcc gatcattatg gtgacggcga aaggggaaga
     7741 agtggaccgt atcgtaggcc tggagattgg cgctgacgac tacattccaa aaccgtttaa
     7801 cccgcgtgaa ctgctggccc gtattcgtgc ggtgctgcgt cgtcaggcga acgaactgcc
     7861 aggcgcaccg tcacaggaag aggcggtaat tgctttcggt aagttcaaac ttaacctcgg
     7921 tacgcgcgaa atgttccgcg aagatgagcc gatgccgctc accagcggtg agtttgcggt
     7981 actgaaggca ctggtcagcc atccgcgtga gccgctctcc cgcgataagc tgatgaacct
     8041 tgcccgtggt cgtgaatatt ccgcaatgga acgctccatc gacgtgcaga tttcgcgtct
     8101 gcgccgcatg gtagaagaag atccagcgca tccgcgttac attcagaccg tttggggcct
     8161 gggctacgtc tttgtaccgg acggctctaa agcatgaggc gattgcgctt ctcgccacga
     8221 agttcatttg cccgtacgtt attgctcatc gtcaccttgc tgttcgccag cctggtgacg
     8281 acttatctgg tggtgctgaa cttcgcgatt ttgccgagcc tccagcagtt taataaagtc
     8341 ctcgcgtacg aagtgcgtat gttgatgacc gacaaactgc aactggagga cggcacgcag
     8401 ttggttgtgc ctcccgcttt ccgtcgggag atctaccgtg agctggggat ctctctctac
     8461 tccaacgagg ctgccgaaga ggcaggtctg cgttgggcgc aacactatga attcttaagc
     8521 catcagatgg cacagcaact gggcggcccg acggaagtgc gcgttgaggt caacaaaagt
     8581 tcgcctgtcg tctggctgaa aacctggctg tcgcccaata tctgggtacg cgtgccgctg
     8641 accgaaattc atcagggcga tttctctccg ctgttccgct atacgctggc gattatgcta
     8701 ttggcgatag gcggggcgtg gctgtttatt cgtatccaga accgaccgtt ggtcgatctc
     8761 gaacacgcag ccttgcaggt tggtaaaggg attattccgc cgccgctgcg tgaatatggc
     8821 gcttcggagg tgcgttccgt tacccgtgcc tttaaccata tggcggctgg tgttaagcaa
     8881 ctggcggatg accgcacgct gctgatggcg ggggtaagtc acgacttgcg cacgccgctg
     8941 acgcgtattc gcctggcgac tgagatgatg agcgagcagg atggctatct ggcagaatcg
     9001 atcaataaag atatcgaaga gtgcaacgcc atcattgagc agtttatcga ctacctgcgc
     9061 accgggcagg agatgccgat ggaaatggcg gatcttaatg cagtgctcgg tgaggtgatt
     9121 gctgccgaaa gtggctatga gcgggaaatt gaaaccgcgc tttaccccgg cagcattgaa
     9181 gtgaaaatgc acccgctgtc gatcaaacgc gcggtggcga atatggtggt caacgccgcc
     9241 cgttatggca atggctggat caaagtcagc agcggaacgg agccgaatcg cgcctggttc
     9301 caggtggaag atgacggtcc gggaattgcg ccggaacaac gtaagcacct gttccagccg
     9361 tttgtccgcg gcgacagtgc gcgcaccatt agcggcacgg gattagggct ggcaattgtg
     9421 cagcgtatcg tggataacca taacgggatg ctggagcttg gcaccagcga gcggggcggg
     9481 ctttccattc gcgcctggct gccagtgccg gtaacgcggg cgcagggcac gacaaaagaa
     9541 gggtaaataa acgggaggcg aaggtgcctc ccgttttgct ttctataaga tactggatag
     9601 atattctcca gcttcaaatc attacagttt cggaccagcc gctaccagcg cggcacccgc
     9661 aggggtgtcg gtgtatttat cgaagttgtc gataaacagt ttcgccaggg tttcggcttt
     9721 ttcctgccac tgttccggag aagcgtaggt gttacgcgga tcgagaatct tcgtgtctac
     9781 gcccggcagt tcggttggga tcgccaggtt aaacatcggc agagtgaagg tttctgcatt
     9841 atccagcgaa ccgttgagga tggcgtcgat aatggcgcgg gtatctttaa tcgagatacg
     9901 tttgccagtg ccgttccagc cagtgttaac cagataagcc tgcgcgcccg ccgcctgcat
     9961 acgtttcacc agcacttctg cgtactgagt cgggtgcagc gacaggaacg ccgcgccgaa
    10021 gcaagcggag aaggttggcg tcggttcggt gatgccacgc tcagtaccgg ccagtttggc
    10081 ggtgaagcca gagaggaagt gatactgggt ttgatcggca gtcaggcgag aaaccggcgg
    10141 caacacgccg aaagcatcag cagtcaggaa gataacctta gtcgcgtggc ccgctttgga
    10201 aaccggctta acgatgttat cgatgtgata gattggataa gaaacgcggg tgttctcggt
    10261 ttttgaacca tcatcaaagt cgatagtgcc atcttcacgc acggtgacgt tttccagcag
    10321 cgcatcacga cggatagcgt tgtagatttc aggttccgct tctttcgaca gcttgatagt
    10381 ttttgcgtag cagccgcctt cgaaattaaa cacgccgtca tcgtcccagc cgtgttcgtc
    10441 atcgccaatc aggcggcgtt tcgggtcggt ggaaagggtg gttttaccgg tgccggaaag
    10501 gccgaagaac accgcaacat cgcctttctc gccaacgttg gcggagcagt gcatagaagc
    10561 gatacctttc agcggcagca ggtagttcat catcgagaac atccctttct tcatttcgcc
    10621 gccgtaccag gtgccgccaa tcagctgcat gcgctcggtc aggttaaacg ccacgaagtt
    10681 ttcggagttc agaccctgtt ctttccactg cgggttagtg cacttcgcgc cgttcataac
    10741 gataaagtct ggtttgaaac ctgccagctc ttcatcgctc gggcgaataa acatgttttt
    10801 gacaaaatgc gcctgccagg ccacttcggt gatgaaacgg acggaaagac gagtatccgg
    10861 gttcgcacca cagaaagcgt cgacaacgaa cagacgtttg ccggaaagct gcctggtcac
    10921 caggcctttc agatgctgcc aggtttccgg agagagaggt ttgttgtcgt tcttaccttt
    10981 gcctttgtct gcccaccaga aagtatcgcg agtggtatcg tcacggacga tatacttatc
    11041 ttttggtgaa cgaccggtga agatcccggt atcgacggca acggcaccca gattagttaa
    11101 caccccgcgc tcataacctg tcaggctcgg atcgagctct tcctgataca gcaggtcgta
    11161 gcttgggttg taaacgatat catgtacgtc actgatacca taagcctcga gttcttgcgg
    11221 gggcaaacca ttgttaacgc gcatttcact gctccttagc caatatgtat tgcctgaata
    11281 gtaaagtctt tttgggggtg ttaaccgcga caaggctcat agatttacgt atctggagaa
    11341 attcatattt gacaaaaaac tccgtgattc ctgtcacgaa acggttgcta ttatcgcaga
    11401 aatagcattt taggtgtgga aaatgttctc aaaaggttaa atcttggttt atatggcggg
    11461 aatattgtga gtggaatcgc attcctgaat gaaattgatg caaattcaac cggtaaagta
    11521 tcgattcacc acgctaactt tttttgatta ttttctgaaa aaagcaaaat actactgccc
    11581 ggatgcgata attaacccac cttttgattc tggataacca acatggataa cgttgaactt
    11641 tcaccggcaa cacgctgggg gatgattgct accggattac ttcaggggat gattgctacc
    11701 ggattacttc aggggctggt ttgctacctg cttatcgcct ggcttgccgg aaaaaatcac
    11761 agttggattg tttatggcgt gcctgcgacc gtcgcgtttt catccgtttt actcttctcc
    11821 gtgatctctt ttaaacagaa gcgcctttgg ggatggctgg cgctggtgtt tattgccacg
    11881 acagggatga gcggttggct gaagtggcaa actgatggca tgaacccctg gagagctgaa
    11941 aaggcgattt gggattttgg ctgctatctg ctgttgatgg caatgttgtt gctaccgtgg
    12001 atacaacaaa gccttcgtat ccgcaatggc agtagccgct atagctactt ttaccagtca
    12061 gtatggcata acgtacttat attactggtg atttttctct ccaatggcct gacgtggctg
    12121 gtgcttttac tgtggggtga gttgtttaaa cttgttggca tcacgttttt caatacgctc
    12181 ttttttgcaa ccgactggtt tatgtatctt acatttggtc tggttaccgc actggcggtg
    12241 atcctcgcgc gtacacagtc acgtttaatc gactctattc aaaagttgtt cacgctaatc
    12301 gccacggggt tgctgccgtt agtatcattg ctaaccctga tgtttatcat caccctgccg
    12361 tttacgggcc tgagcgcgat ttctcgccac atctccgccg ccgggttgct gttaacgctg
    12421 gcctttttgc aattgatctt aatggctatt gtccgcgatc cgcaaaaagc gtcacttccc
    12481 tggacggggc cgttgcgttg cctgattaaa accgctttgc tggttgctcc gctgtatgtg
    12541 ttcgtcgcca cctgggggtt atggctgcgg gtcgctcagt acggctggac tgtcgaccgc
    12601 ttgcagggcg cgctggcgga gctggtgtta ctggtgtggt cgctggggta ttttgtcagt
    12661 atcgtctggc gtaatgggca aaatcccctt gttcttcagg gcaaagtgaa ccttgcggtt
    12721 tcgttattgg tgttggtgat actggtgctt cttaattcgc cggtgctgga cagtatgcgt
    12781 attagcgtga acagtcatat ggcgcgttat cagagcggca aaaacacgcc agaccaggta
    12841 actatctaca tgctcgaaca gagcggtcgc tatggacgtg cggcgcttga gtcgctgaaa
    12901 agcgatgccg ggtttatgaa agacccgaaa cgcgcgcggg atctgctgat ggcgttagat
    12961 ggagagcaac atcttcagga acaggtatcg gaaaaagtat tcgccgaaaa tgtgttaatt
    13021 gcccctggtt ctgttaaacc ggatgcgaca ttctggtcgg ccttaatcca ggatcgctat
    13081 aacgtgatga cctgtattga aaaagacgcc tgcgtactgg tcgagcaaga tctgaatagt
    13141 gatggtcagg cggagcggat cctgtttgct tttaatgatg acagagtcat tgtctatggc
    13201 tttgactcag acagaaaaga atgggacgcg cttgatatga gtttacttcc gaacgaaata
    13261 acgaaagaaa aattactcac agctgcgaag gatggaaaac tggggacgag gccgaaagcg
    13321 tggcgcgatc tgacggtaga tggtgaaacg ctggaaataa acttaagtaa gtgacaacgc
    13381 ttttatcgga tacgacgctg tatctgacca aaataatttg cgatttattt cataggcgct
    13441 ggtaagcgca gcacatcagg ccattaagtc gtaaaatccg gcagagccct ctctgccgga
    13501 catactcatt aatgaactta cggatctgcc ggagacgcgt tgttgcggat ttcagcaata
    13561 tccatcgcat tgaacagata gtggttaccg cagtaatcac aatgcatgtc aatttcgcca
    13621 tcttccgcca ggatgctatc aacttcttca tcaggcagcg ttttcagcgc atcggcgcaa
    13681 cgttcacgcg agcaggtgca tttgaactcc acatcctgcg gatcgtaaac cgtcacctct
    13741 tcttcgtgat acaaacgcca caacacttcg tttgccggta aggtcagcag ttcttcggtt
    13801 ttgatggttt cggttagcgt cgccaggtgg tcaaagtcgt cctgttgggt attttgcgca
    13861 ggcattacct gcaacaacat accgcctgca gccggtttgc cgtctacgtc gccggtgcga
    13921 ataaacaggc gcgtcggcag ctgttcagaa cgcataaagt aatcttctag gcaggccgcc
    13981 agggtatcac cttccagacc aactacgccc tgatagcgtt cgccttcgct cggggtaatg
    14041 gtgatcacca cgtaaccatt gccgaccagc gttttcaggt cggcattttc tggaatttcg
    14101 ccctgcacgc gcgccacacc gcgcatttgc tggttattgt taccgttaat aaccgccaga
    14161 ttcatcggac cgtcgccctg cagctgtacg gtgatatcac catcaaactt cagcgtagcg
    14221 gttaacaggc tggtcgcaac cagcagttct gccagcacgt ttttaacggg ctgcggataa
    14281 tcgtggttct caaggatctg ttgcagggtt tccgaaacgg ttaccagttc gccgcgaacg
    14341 gcaaaatttt caaacagata gcgatgtaat tggtcatgtt gcggcataat catctctctt
    14401 gcaggtgaca gttattcact gtcgccgtgt ttaaatcgta acaggtcgcg gcgctctttt
    14461 ttgtccgggc gtcggtccgg gtgcggcatg gttaaggcat taagtttacg tgccatcgcc
    14521 attttttcgc gtttctctac actttccgca gtctcttcat acagcaaggc tgcctcgctg
    14581 gcggggcgac gctgttcagt aatcgccttt acaatcaccg tgcgttcgtc atttccctgg
    14641 cgcagagtga gcgtggcatt cagctcgacg attttgctcg gcttgctgcg ctgcccgttg
    14701 taatgcacct taccgccttc aatcatttca cgggccagcg cgcgggtttt ataaaaacgg
    14761 gcagcccata gccatttatc cagtcgaacc tcaacagcag gtttctcttt catggcgtct
    14821 ccttcacatt agcgagggga tcaggcggcg gtagtcattc agtgacggat ggcgttgata
    14881 ctgtttctcg gcaatcccgg aatcaggatt agtcacgccg aggcagtaac gaataccaaa
    14941 ttgcgcggca gcatcgagaa tcgcttcgct gtcatcaata aacagcgttc tttcagcttt
    15001 cagacccgta gcttcggcca ccgcatgcca taaccgctga tcctctttcg gataaccaaa
    15061 tgtgtgggtg gaaagtaata aatcaaggtg tgcgtccaga cccgtatgct caagttttac
    15121 cgccaggttg tgcggatgag cattggtgag caaaattcgc tgcttaccgc tggctttcag
    15181 tgcctcaaga aacggaatgg tatcttcacg cagtacggcg cgcggtccca tctcggtggt
    15241 catcgcacag atatccagac ccagttgctc actccagtaa tcaagacagt accagtttag
    15301 cgtatgctgt acgtcgtgat attgctggcg catatattcc atcgcttcct gtggcgtaac
    15361 cccgtttttc gcgccccatg tttcaggcac cagcttttgc cagaaatagt tatcgaaggc
    15421 gaggtcgagc aacgtgccgt ccatatccag cagaacggta tctacgtcct gccaggcaat
    15481 gttgatatgc atgagggaaa tctccagagt gaagcaattt gcgcgacagg gtagcataac
    15541 ctgccgcgca aacgtgttat tcgataaggc tttctgaagg ggtgatcagt tgcgggttca
    15601 ggcagctttc ataataggcc tgaatctcca tcatgcgagt gcgatgacgc tggtaacgac
    15661 gccaggcctg tacgccattg taaatggcgc tacccagcat cgtcagtagc agcaatgtgg
    15721 tgctgagata gcgccacagg ccggaacgat ccgggatcgg atgaaggcca atatgctgcg
    15781 taccattggc gtcagtgaag atttttgtga cgataccttc ggcgttaaac ggcgtatgca
    15841 tcagcatttg tgccagtttc tggaaagcgt tccactgttc ttgcggcggg tagtcgtaaa
    15901 gtgatgccga aggccagggc tgatcaacaa aatcgctgcc ttcgtcgctg acaatcagga
    15961 atccgccggg ggccggactg tttagtgatt gtgccgctcg cgccgtttca tgcgtgataa
    16021 acggcgcggt ggaggttgcc accaggttat ccagcgattc agcactcacc gggcgtaata
    16081 acacattcac gccatcgagc ttcccggcgt tggcgcgttt taccagcgcg tcccagtctt
    16141 tactgttgcc gagattgacc agcgcatttt tcagtcgcac acagtcatct ttggcagaac
    16201 ataaatccgc tgtcttcagt acaatgtcgc caaaatcatc aagcaatacc atgccggatt
    16261 tttgaatcgc tgaacgtaat gaggcactga cgcgagattc atcttccggt ttagggtgca
    16321 gctggcgatt aactgcttca gtcaatgccg tcgctttgtt gaccagttca gattctggta
    16381 atggtaatga gcgggcgtca ttccagatga tctgcgagca gtcaaacggt aaaaaaggtg
    16441 aattggtttt cgcgctccag gttccggaag tgcgaatatt acacattccc gtaccgctaa
    16501 tacgcaatgt atcgcctacc cgcacgccag cgtcagccag ttgttttacg ctggtggctt
    16561 caatggtctg cgcgcctttc atccatgaga gagtgaattt cagcggcata tccagcggga
    16621 tccagaataa cagcataaac agcactagca gcgagcccgc agcgataatc gtactgcgca
    16681 gccagtgctg taacggaaag ttttttactt catcatgcag cgaaagatat cgcccctgac
    16741 gcactacgtg gcggtcgaga tagatatcaa tatcggtttg ttgaccgaga tcctgagcaa
    16801 tgtatggctg ccagtgtgcg ggatagacca ggtcgataat accgagtgaa atattgttga
    16861 tctgttcctg atcgttttcg ccaaacaatc cccaacgacg gggtgtaccg cgcagacaat
    16921 gaatttctcg cagggaggat tttgccgggg gcgcgaatag cccccacaga cctgcgccca
    16981 gcagcagtaa tgcgccgcct gccagccacg gaacaaatac atccggggta atcaggcaga
    17041 aaaagaacat caggaaggag gcgacgatca gcaacgcttc acgcagcccg cgcggacgac
    17101 tcagcgcata ttcttcatgc gtttctttgc gaatattgag tagctcgatt tgctcactct
    17161 cttccccgcg aatcgacgcc tgcgtggacg gaacaggttg caaggcatag ccgcgcgttt
    17221 cctgcatgta ctcctgcagc gtatgaccgt tgagtgaaat aaccaacggc agcgaatcgg
    17281 tatgaatcag ttcaacggta ttctcatcgt tgatgtactg ttcccagaag gggggcaggt
    17341 ggacttctac cgaatcgagg tagtaacgcc atttattcgg atcgtcggta gagatgccgt
    17401 aacgtgtgat agcgtgtgtc agcatcatga cgttgttgct ttcggcattc agcgccagag
    17461 agatcggtgc cgcgctagct tccgttgggc caggcacctg taatacctgc gtcaggctct
    17521 caagataatt ttcaacggcg ctacgttctt caggtgtgag tttacgcgtt tgcgcatccg
    17581 cgaaggcgtt ggtccagggc agctgacgcc gtctggatcg cacttttatc agccatcccg
    17641 caagtagtga gcaggccagc aaagcagcta aaaaaatcac aatggtgctc atgctttccc
    17701 catcttactt agtctgtcag gcgtggtcag tcgcaccgga cacaatacat gattctgcgg
    17761 ttggctatcg gcaagcgtgg agcttaactt gagcatcatt aagcacatca cagtaaccgc
    17821 tgatcatagc aaaccctctg aaggcggaat atcagcaaat tcctggagtt atttcaacct
    17881 attgactttt aatttgattt gaggacgatt atttattaag ttacataaat gtaaataaca
    17941 tgcaattcac attgccgaaa gcgtgtccga tatcgcacaa taaccgctga tttcacagca
    18001 tagatcccac gatgaacaaa tcattacaaa aacccaccat tctgaatgtt gaaactgtag
    18061 cccgttcccg actgtttacc gtcgagagcg tggatctgga gttcagcaat ggcgtgcggc
    18121 gtgtttatga acgaatgcgt ccaaccaacc gggaagcagt gatgattgtg ccgattgtgg
    18181 acgatcacct gatcctgatc cgcgaatacg cagtgggaac tgaatcctac gaattaggtt
    18241 tttcgaaagg attaattgat ccgggtgaaa gcgtctacga agccgctaac cgcgagctaa
    18301 aagaagaggt tggatttggt gcgaacgatc tgactttttt gaagaagctc agcatggcac
    18361 cgtcttactt ttccagcaaa atgaatatcg tggtcgcgca agatctctac ccggaatcgc
    18421 tggaaggcga tgagccagag ccgctaccac aggtgcgctg gccgctggcg catatgatgg
    18481 atttgctgga agaccctgac ttcaacgaag cgcgtaatgt cagcgcgctg ttcctcgtgc
    18541 gcgaatggtt gaaagggcag gggcgagtgt aaatgcgtga agtttgcctg atgcacaacg
    18601 cttatcaggc ttatggggtt cctgcaatat attgattttt cacgattttg ttagccggat
    18661 aacgcgttca cccgcatccg gcataaacaa atgcactttg tcagccaact gaaaaggcgc
    18721 cgaagcgcct ttttaatcag aacaattcct gtgcctcacc attatcgata atggtcgttc
    18781 ccacctcgtg caccgcctgt tgtgtcggct gcgtaccttc gatgaaatac tcttcgcggc
    18841 tgttgccacc attagctaac tgcccggtgc tgcgatcgat attcaccgtc acaatacccg
    18901 gaggcggcgt cagcggctgc tccggcacac cttcaagaac ggctttcata taagcgtccc
    18961 atgcaggctg ggcactcttg gcaccgcctt cgtaacctga gatctgatct ttaatcgctc
    19021 cggaagccgt tgtatgaccg agattacgac ggtgatcatc aaagccaatc cagaccgagg
    19081 tcacaacgcc cggaccgtaa cccgagaacc acgcatcttt cgaactgtta gtggtaccgg
    19141 ttttcccgcc gatatcgcga cgctgcaaat cacgacctgc acgccagccg gtaccctgcc
    19201 agcctggttc accaaagata ttggtgttca aagcactctt aatcaggaat gccagcggag
    19261 tgttgatgac gtgcggtgcg tactcctgcg cgccagtctt cgccactaac gcctgatttg
    19321 cctgctccag ctgcggcatt ggtacagaaa cattctgctg ctcgcgggag atagcgacat
    19381 cttcaacatc gttattttcc agcacgttcg atttctgcgt atcaccgtaa atcactggaa
    19441 tatcgcattc cgggcaggct actttcggtt tcgcttcgaa aatcacgccg ccctgatcgt
    19501 tttcaatttt gctgataaac cacgggtcca ctaggaagcc gccgttcgcc atgaccgcgt
    19561 agccgcgcgc cacctgcatt ggggtgaagg acgctgaacc cagcgccagc gattcggtgt
    19621 ggacaatgtt ttgcgccggg aagccgaagc gttgcagata ttctgcagcg tagtcgacgc
    19681 ccatcgcccg cattgcacgt accatcacca cgtttttcga ctgacccagc ccctgacgta
    19741 agcgaattgg accagcatac tgcggtggtg agttcttcgg ctgccagtca gaaccggcac
    19801 ctgcatccca gcgagaaatt ggcacatcgt tcaacatact tgccagcgtc agacctttat
    19861 ccatcgccgc ggtgtagagg aacggtttga tgttggaacc cacctgacgc agtgcctggg
    19921 tggcgcggtt aaacttgctc tgattgaaat caaagccacc gaccagcgcc ataacggcac
    19981 cattttgcgg attgatcgac accagcgccg agttcacttc cggcacttgt gccagccacc
    20041 atgcatcgcc aacctgacga acccagattt gctgacccgt ttgcagaaca tcggtcactt
    20101 tacgcggcgt cggtccctgc tgagtatccg aacggtaagg acgcgcccag cgaacgcctt
    20161 ccatactcaa tgcgacggtc gacccgtccg ccagcatcgc cgtcgctccc tgaggattgg
    20221 cgctggtgac tgcggcaggc agcagcggac cataggtcgg cagcgccttc agcgtatcgg
    20281 taatcttgtt gttatcccac gccgactcgc ccactttcca cagcacattt gccgggccgc
    20341 gatagccgtg gcgcatgtcg tagtccagca cgttattacg taccgcctgc tgcgcggcct
    20401 gctgcacttt gcgggtgatg gtggtgtaaa tgcgataacc gtcttcatag gcactttcgc
    20461 cataacggtt atacatctcc tggcgcacca tttcgctcag gtacggcgca gagaaagcaa
    20521 tctccggcgc gtgataatta gcgttaatcg cctcagtgcg tgtctgatcg aactgttgct
    20581 gggtgatata cccttcatcc agcatccgcg acagtacgac gttacgccgc gcgacggcac
    20641 gatccattga gtagagcgga ttaaaggtgg aaggcgcttt cggcaacccg gctatcaccg
    20701 ccatttcgtt cagcgtcagt tggtcgaccg ttttaccgaa atagacttgt gccgcagcac
    20761 cgacaccata ggcgcggtaa ccaaggtaaa tcttgttcag ataaagctcg aggatttcgt
    20821 ctttcgtcag caactgttca atgcgaatcg cgaggaagac ttccttaatc ttacgcatca
    20881 tcgtgcgttc tggactgagg aagaaatttc tcgccagctg ctgggtaatg gtactcgccc
    20941 cttgtgacgc gtggccggag aacagcgcca cacttgccgc acggaagatc cccaccgggt
    21001 caacgccgtg atgctcgtag aagcggctgt cttccgtcgc gataaaggct ttcaccatct
    21061 ccggtgggat ttgatccaac gtaaccggaa tacgacgttt ctcaccgtat tgagcaatca
    21121 gctcgccatc ggcgctgtaa atctgcatcg gaatttgcag gcgaacatct tttaatgtca
    21181 ccacatccgg cagttgtggc tcgatgtagc ggtataggcc ataaatcgag cctgctccca
    21241 gcagaatgca acagactgca aggatcaaaa aatactttac gaacttcact ggaaatttcc
    21301 catttagttt catttgggca gtttataaac aaacgcgcgg tagtataaag gcaagccaga
    21361 cgcattgata tacccgtcag agtgacgggt gataaggaga tcatcacaat gacatttaag
    21421 atctggcaaa ttggtttgca tttacaacag caagaagcgg cagcggttgc gatcgtgcgg
    21481 ggcgcaaaag aatgcttttt gcaacgctgg tggcggttgc cgctggcgca cgacattatc
    21541 aaagatgggc ggattgttga tgcgcagcag ctggctaaaa cgttgttacc gtggagtcgc
    21601 gaactgccgc agcgtcatca cattatgttg gcgtttcccg ccagtcgcac attacagcgg
    21661 tcatttccgc gcccgtcgat gtcccttggt gagcgggagc aaacggcctg gctgtcaggg
    21721 acgatggccc gcgagctgga tatggatccg gactccctgc gcttcgatta cagcgaagac
    21781 tcactcagcc ccgcttataa cgtgactgcc gcgcaaagca aagagctggc aacgctgctt
    21841 acgctggcag aaaggttgcg tgttcatgtt agtgcgatca ccccggatgc cagtgcatta
    21901 cagcgattcc tgcctttttt accttctcat cagcaatgtc tggcctggcg tgataacgaa
    21961 cagtggctgt gggcgacgcg ctatagctgg gggcgcaaac tggcggtggg gatgactagc
    22021 gcgaaggagc tggcggcagc gttatccgtt gatcccgaca gcgtcgcgat atgtggcgaa
    22081 ggcggatttg acccctggga ggccgtttct gttcgtcagc cgccgctacc gccgcctggt
    22141 ggagactttg ccatcgcgct ggggctggcg cttgggaagg cgtactgatg aatccgccaa
    22201 ttaatttttt gccctggcga cagcaacgcc ggaccgcttt tctgcgtttc tggttgctga
    22261 tgttcgttgc gcctctgctg ctggccgtcg ggataacgct aatactgcgt ctgacaggca
    22321 gcgccgaagc gcgcatagac gcagttttgc ttcaggcgga acaacaactc gcccgcagct
    22381 tacagataac gaagccacgt ttgctggagc aacagcagtt acgcgaacag cgttcgcaac
    22441 ggcagcgcca gcgacaattt acccgcgact ggcaatctgc gctggaagca ctggcggcgc
    22501 ttttacccga gcacgcctgg ctgacaacga taagctggca gcagggaacg ctggagatca
    22561 aggggctgac aacaagcatt accgcgttaa acgcactaga aacgtcactc cgccaggatg
    22621 cgtcttttca tctcaatcag cggggagcca cgcagcagga tgcgcaggga cgctggcaat
    22681 ttgagtatca gttaacaagg aaggttagcg atgaacatgt tctttgactg gtggttcgcc
    22741 acatcacccc gcctccgcca gttttgctgg gcattctggt tgctgatgtt agttacgctc
    22801 atttttctat catcgacaca ccatgaagag cgcgacgcat taattcgact acgggcaagt
    22861 catcaccagc agtgggccgc actgtatcgc ctggtagaca ccactccctt cagcgaggaa
    22921 aaaacgctgc ccttttcgcc actggatttt cagttatccg gcgcgcaact ggtttcctgg
    22981 catccatccg cgcagggagg tgagttggcg ttgaaaacgc tgtgggaagc agtgccgtcg
    23041 gcatttacac ggctggcaga gcgcaacgtc agcgtgagcc gtttttcgtt aagcgtggaa
    23101 ggtgatgatc ttttgttcac gctacaactg gagatgccgc atgagggtta aacgctggtt
    23161 gttggcaggt attgcattgt gccttttaac cggtatgcgt gaccctttta aatcgccgga
    23221 agatctatat gccggattag cgaacttagc cagtggcgct atcaggggat ggtagggcga
    23281 ggcgagcgca tcatcggtgt aataaaagac gggcaaaaga aatggcgacg ggtgcagcaa
    23341 aacgatgtgc tggaaaacgg ctggacaatt ttacagctga cgccagacgt actaacgctg
    23401 ggtaccggga caaactgcga accgccacaa tggttgtggc aacggcaagg agatacaaat
    23461 gaagcaatgg atagccgcac tactgttgat gctgataccc ggcgtacagg cggcaaagcc
    23521 gcaaaaagtg acgctgatgg tggatgacgt tccggtagct caggtgttgc aggcgctggc
    23581 tgaacaggag aagttgaacc tggtcgtgtc gccagacgtc agcggtacgg tgtcgttaca
    23641 tctaacagat gttccctgga agcaggcact acaaactgta gtgaaaagcg ccggactgat
    23701 aacgcggcag gaaggcaaca ttctctcagt gcattccatt gcctggcaga atgacaatat
    23761 cgcccgccag gaggcggagc aggcgcgggc gcaggcaaat ctgccgctgg aaaatcgcag
    23821 tataaccctg caatacgccg acgcgggaga actggcgaaa gcgggggaga agctactgag
    23881 tgccaaaggg agtatgaccg tcgataaacg caccaatcgc cttttgctac gagataacaa
    23941 aacggcgtta agcgcgcttg aacagtgggt agcgcaaatg gatctgccgg tcgggcaggt
    24001 tgagctgtcg gcgcatattg tcaccattaa tgaaaaaagt ttgcgtgagt taggcgtgaa
    24061 atggacgctg gccgatgcgc aacacgctgg tggcgttggg caagtcacca cgcttggtag
    24121 cgacctctcc gtagcgacgg cgacaacgca tgtcggtttt aacattgggc gcatcaacgg
    24181 acgcttgctg gatcttgagc tttccgcgct cgaacaaaaa cagcagctgg atattatcgc
    24241 cagtccgcgt ctgctggcct cacatcttca gcctgccagc attaaacagg agagcgaaat
    24301 tccatatcag gtttccagcg gggaaagtgg cgcgacgtcg gtggaattta aagaggccgt
    24361 cctggggatg gaggtcacgc ccacggtgtt acaaaaaggt cgcatccggc tgaaattaca
    24421 catcagccag aacgttccgg ggcaggtgct acagcaggcc gatggcgaag tgctggcgat
    24481 tgataagcag gagatcgaaa cgcaggtcga ggtaaaaagc ggagaaacgt tggcgctggg
    24541 cggcattttt acccgtaaaa ataaatcggg tcaggatagc gtaccgttgc ttggcgacat
    24601 tccctggttc gggcaattat ttcgtcatga cggaaaagaa gatgaacgac gcgagttagt
    24661 ggtgtttatc acgccacgac tggtttccag tgagtaaaca gccgtaaaag cggtaatgtt
    24721 tttatgctga acgtgtttca tctatttgac gcgcgcaggt atttagcata caaggagtac
    24781 tgatttgaga gttggtgctc ttcgctgcct gcgttccatg atgatgattt atcattcagg
    24841 cggcattttg ctgtcttttt tacgctaatc ttacccggtg atttatcgcc agagcggtgg
    24901 tagcaaggca gcgcgcttgc agcgaccaga tatgcagagg gatgggtgat ttattcagtt
    24961 gccaaacccg ctggagtatt gagataattt tcagtctgac tctcgcaata tcttatgagg
    25021 tttcagttca tgtcctgcgg cgctctctga gcgaagcggg tttatcatta acgaatagtc
    25081 ttagtagtac cgaaaaaatg gcagagaaac gcaatatctt tctggttggg cctatgggtg
    25141 ccggaaaaag cactattggg cgccagttag ctcaacaact caatatggaa ttttacgatt
    25201 ccgatcaaga gattgagaaa cgaaccggag ctgatgtggg ctgggttttc gatttagaag
    25261 gcgaagaagg cttccgcgat cgcgaagaaa aggtcatcaa tgagttgacc gagaaacagg
    25321 gtattgtgct ggctactggc ggcggctctg tgaaatcccg tgaaacgcgt aaccgtcttt
    25381 ccgctcgtgg cgttgtcgtt tatcttgaaa cgaccatcga aaagcaactt gcacgcacgc
    25441 agcgtgataa aaaacgcccg ttgctgcacg ttgaaacacc gccgcgtgaa gttctggaag
    25501 cgttggccaa tgaacgcaat ccgctgtatg aagagattgc cgacgtgacc attcgtactg
    25561 atgatcaaag cgctaaagtg gttgcaaacc agattattca catgctggaa agcaactaat
    25621 tctggcttta tatacactcg tctgcgggta cagtaattaa ggtggatgtc gcgttatgga
    25681 gaggattgtc gttactctcg gggaacgtag ttacccaatt accatcgcat ctggtttgtt
    25741 taatgaacca gcttcattct taccgctgaa atcgggcgag caggtcatgt tggtcaccag
    25801 cgaaaccctg gctcctctgt atctcgataa ggtccgcggc gtacttgaac aggcgggtgt
    25861 taacgtcgat agcgttatcc tccctgacgg cgagcagtat aaaagcctag ctgtactcga
    25921 taccgtcttt acggcgttgt tacaaaagcc gcatggtcgc gatactacgc tggtggcgct
    25981 tggcggcggc gtagtgggcg atctgaccgg cttcgcggcg gcgagttatc agcgcggtgt
    26041 tcgtttcatt caagtcccga cgacgttact gtcgcaggtc gattcctccg ttggcggcaa
    26101 aactgcggtc aaccatcccc tcggtaaaaa catgattggc gcgttctacc agcctgcttc
    26161 agtggtggtg gatctcgact gtctgaaaac gcttcccccg cgtgagttag cgtcggggct
    26221 ggcagaagtc atcaaatacg gcattattct tgacggtgcg ttttttaact ggctggaaga
    26281 gaatctggat gcgttgttgc gtctggacgg tccggcaatg gcgtactgta ttcgccgttg
    26341 ttgtgaactg aaggcagaag ttgtcgccgc cgacgagcgc gaaaccgggt tacgtgcttt
    26401 actgaatctg ggacacacct ttggtcatgc cattgaagct gaaatggggt atggcaattg
    26461 gttacatggt gaagcggtcg ctgcgggtat ggtgatggcg gcgcggacgt cggaacgtct
    26521 cgggcagttt agttctgccg aaacgcagcg tattataacc ctgctcacgc gggctgggtt
    26581 accggtcaat gggccgcgcg aaatgtccgc gcaggcgtat ttaccgcata tgctgcgtga
    26641 caagaaagtc cttgcgggag agatgcgctt aattcttccg ttggcaattg gtaagagtga
    26701 agttcgcagc ggcgtttcgc acgagcttgt tcttaacgcc attgccgatt gtcaatcagc
    26761 gtaacaacaa gaaaggtcag gccgcttatc aagcggtcta ttagcttcag gttaattgca
    26821 acgtggtaag cattaacctt ttagtggggt gttaaatgga tgaattcaaa ccagaagacg
    26881 agctgaaacc cgatcccagc gatcgtcgta ctggtcgttc tcgtcaatct tctgaacgtt
    26941 ctgagcgtac tgaacgtggc gaaccgcaga tcaattttga tgatattgaa cttgatgaca
    27001 ctgacgatcg ccgtccgact cgtgcgcaaa aagagcgcaa tgaggaaccg gaaatcgaag
    27061 aagaaattga cgaatccgaa gatgaaaccg tggatgaaga gcgcgtagag cgtcgtccgc
    27121 gtaagcgcaa aaaagcagcc agtaaacccg cttctcgtca gtatatgatg atgggcgtcg
    27181 gcattctggt tctactgctg ttgatcatcg gtatcggttc tgcgctaaaa gccccctcga
    27241 ccacttccag cgatcaaacc gcgtctggcg agaagagtat tgatcttgct ggcaatgcga
    27301 ccgatcaggc gaatggcgtg cagccagcgc cgggaaccac gtctgcggaa aatactcagc
    27361 aggatgtttc tctgccaccg atctcttcta cgccgactca agggcaaacc ccggtggcaa
    27421 cggatggtca acaacgtgtt gaagtgcagg gtgacctgaa caatgcgctg acccagccac
    27481 aaaatcagca acagctgaac aatgtggcgg tcaattccac gttgccgact gaacccgcaa
    27541 cggttgcgcc tgttcgcaat ggcaatgcat cgcgtgacac ggcgaaaacg caaaccgctg
    27601 aacgtccgtc cactacgcgc ccagttcgtc agcaggcggt gattgaaccg aaaaaaccgc
    27661 aagcaaccgt gaaaacggag ccgaagccgg tagcacagac gccgaagcgt actgaacctg
    27721 aaccagctgc ccctgtggcg agcacgaagg caccggctgc gacttctacg ccagcaccaa
    27781 aagagacggc gactacggct ccagtacaga cggcatcccc ggcgcaaacc acggcaacac
    27841 cagccgctgg agggaagacc gcaggtaatg ttggttcgtt gaaatcggca ccgtccagcc
    27901 attacactct gcagctgagc agttcctcta actacgacaa cctgaacggt tgggcgaaga
    27961 aagagaatct gaaaaactac gttgtctatg aaacgacgcg taatggtcag ccgtggtatg
    28021 tcctggtttc tggcgtgtac gcttcgaaag aagaggcgaa aaaagcggta tctacattgc
    28081 cagcagatgt tcaggccaaa aacccgtggg cgaaaccgct gcgtcaggta caggccgatc
    28141 tgaagtaatc aaggttatct cccgcaatgg tttatcgttg cgggagctgc ctgaagcgct
    28201 ggatgctgtc ggagctttct ccacagccgg agaaggtgta attagttaat cggcatgaag
    28261 aaaaatcgcg cttttttgaa gtgggcaggg ggcaagtatc ccctgcttga tgatattaaa
    28321 cggcatttgc ccaagggcga atgtctggtt gagccttttg taggtgccgg gtcggtgttt
    28381 ctcaacaccg acttttctcg ttatatcctt gccgatatca atagcgacct gatcagtctc
    28441 tataacattg tgaagatgcg tactgatgag tacgtacagg ccgcacgcga gctgtttgtt
    28501 cccgaaacaa attgcgccga ggtttactat cagttccgcg aagagttcaa caaaagccag
    28561 gatccgttcc gtcgggcggt actgttttta tatttgaacc gctacggtta caacggcctg
    28621 tgtcgttaca atctgcgcgg tgagtttaac gtgccgtttg gccgctacaa aaaaccctat
    28681 ttcccggaag cagagttgta tcacttcgct gaaaaagcgc agaatgcctt tttctattgt
    28741 gagtcttacg ccgatagcat ggagcgcgca gatgatgcat ccgtcgtcta ttgcgatccg
    28801 ccttatgcac cgctgtctgc gaccgccaac tttacggcgt atcacacaaa cagttttacg
    28861 cttgaacaac aagcgcatct ggcggagatc gccgaaggtc tggttgagcg ccatattcca
    28921 gtgctgatct ccaatcacga tacgatgtta acgcgtgagt ggtatcagcg cgcaaaattg
    28981 catgtcgtca aagttcgacg cagtataagc agcaacggcg gcacacgtaa aaaggtggac
    29041 gaactgctgg ctttgtacaa accaggagtc gtttcacccg cgaaaaaata attctcaagg
    29101 agaagcggat gaaacagtat ttgattgccc cctcaattct gtcggctgat tttgcccgcc
    29161 tgggtgaaga taccgcaaaa gccctggcag ctggcgctga tgtcgtgcat tttgacgtca
    29221 tggataacca ctatgttccc aatctgacga ttgggccaat ggtgctgaaa tccttgcgta
    29281 actacggcat taccgcccct atcgacgtac atctgatggt gaaacccgtc gatcgcattg
    29341 tgcctgattt cgctgccgct ggtgccagca tcattacctt tcatccagaa gcctccgagc
    29401 atgttgaccg cacgctgcaa ctgattaaag aaaatggctg taaagcgggt ctggtattta
    29461 acccggcgac acctctgagc tatctggatt acgtgatgga taagctggat gtgatcctgc
    29521 tgatgtccgt caaccctggt ttcggcggtc agtctttcat tcctcaaaca ctggataaac
    29581 tgcgcgaagt acgtcgccgt atcgacgagt ctggctttga cattcgtctg gaagtggacg
    29641 gtggcgtgaa ggtgaacaac attggcgaaa tcgctgcggc gggcgcggat atgttcgtcg
    29701 ccggttcggc aatcttcgac cagccagact ataaaaaagt cattgatgaa atgcgcagtg
    29761 aactggcaaa ggtaagtcat gaataagttt gaagatattc gcggcgtcgc ttttgatctt
    29821 gatggtacgc tggtcgacag tgctcctggt cttgctgctg cggtagatat ggcgctgtat
    29881 gcgctggagt tgcccgtcgc aggtgaagaa cgcgttatta cctggattgg taacggcgca
    29941 gatgttctga tggagcgcgc attgacctgg gcgcgtcagg aacgtgcgac tctgcgtaaa
    30001 acaatgggga aaccgcccgt tgatgacgac attccggcag aagaacaggt acgtattctg
    30061 cgtaaactgt tcgatcgcta ctatggcgag gttgccgaag aggggacgtt tttgttcccg
    30121 cacgttgccg atacgttggg cgcgttgcag gctaaaggcc tgccgctagg cttggtcacc
    30181 aacaaaccga cgccgttcgt cgcgccgctg ctcgaagcct tagatattgc caaatacttt
    30241 agcgtggtta tcggcggcga tgatgtgcaa aacaaaaaac cgcatccgga cccgctgtta
    30301 ctggtggctg agcggatggg aattgcccca caacagatgc tgtttgtcgg cgactcacgg
    30361 aatgatattc aggcggcaaa agcggcaggt tgcccatcag ttggcttaac ctacggatac
    30421 aactacggcg aggctatcga tctcagccag cctgatgtaa tttatcagtc tataaatgac
    30481 cttctgcccg cattagggct tccgcatagc gaaaatcagg aatcgaaaaa tgactaagcc
    30541 catcgttttt agtggcgcac agccctcagg tgaattgacc attggtaact acatgggtgc
    30601 gctgcgtcag tgggtaaaca tgcaggatga ctaccattgc atttactgta tcgttgacca
    30661 acacgcgatc accgtgcgcc aggatgcaca gaagctgcgt aaagcgacgt tggatacgct
    30721 ggccttgtat ctggcttgtg gtatcgatcc tgagaaaagc accatttttg ttcagtccca
    30781 cgtaccagaa catgcgcagt taggctgggc actgaactgc tatacctact tcggcgaact
    30841 gagtcgcatg acgcagttta aagataaatc tgcgcgttat gccgagaaca tcaacgctgg
    30901 tctgtttggc tatccggtgc tgatggctgc ggacatcctg ctgtatcaaa ctaatctggt
    30961 accggtgggt gaagaccaga aacagcacct ggaactgagt cgcgatatcg cccagcgttt
    31021 caacgcgctg tatggcgaga tctttaaggt gccggagccg tttattccga aatctggcgc
    31081 gcgcgtaatg tcgctgctgg agccgaccaa gaagatgtcc aagtctgacg ataaccgcaa
    31141 taacgttatc ggcctgctgg aagatccgaa atcggtagtg aagaaaatca aacgcgcggt
    31201 cactgactcc gacgagccgc cggtagttcg ctacgatgtg cagaacaaag cgggcgtttc
    31261 caacctgctg gatatccttt ctgcggtaac gggccagagc atcccagaac tggaaaaaca
    31321 gttcgaaggc aagatgtatg gtcatctgaa aggtgaagtg gctgatgccg tttccggtat
    31381 gctgactgaa ttgcaggaac gctatcaccg tttccgcaac gatgaagcct tcctgcaaca
    31441 ggtgatgaaa gacggcgcag aaaaagccag tgtacacgct tcccgtacgc tgaaagcggt
    31501 gtacgaagcg attggttttg tggcgaagcc gtaatatcaa aacgctcgga cggtcccctc
    31561 gcccctttgg ggagagggtt agggtgaggg gagcaggcaa gcgccagtgg gaattaccct
    31621 ttgctcgcat tgtcgaataa atcacttcgt catcactctg aaaccgggga aacccggttt
    31681 ttttatcctc ttttcgcaac aacccgaaaa aatgtgaagc gcctcgccgt ttccacatct
    31741 cgtcgtcgcc acttatcttc attttaatga agatataaat attcatttta ttgaaaatat
    31801 attatgcgtc gaacgtttat caaaaaagaa ggcgtcgtca tcacgacact ggcccgttat
    31861 ttgttgggtg aaaaatgcgg taatcgactg aaaaccatag atgagctggc aactgaatgc
    31921 cgttcatcgg ttggcctgac gcaggccgcg ttgaaaacgc tggaatcaag cggagcgata
    31981 cggattgaac gccgtgggcg caatggcagt tatcttgtcg agatggataa caaagcattg
    32041 ctgagtcatg tggatatcaa caacgtggta tgtgcgatgc ctttgcccta tacccgcttg
    32101 tacgaaggtt tagcgagcgg attgaaagcc cagtttgatg gcattccttt ttactatgcg
    32161 catatgcgcg gcgcggatat tcgcgtggag tgtttactta acggtgtcta tgacatggca
    32221 gtagtttccc gcctggcggc ggaaagttat ctcagccaaa acaatttgtg catcgccctc
    32281 gagcttggcc cgcatacgta tgttggtgaa catcaactga tatgccgcaa aggtgagtct
    32341 ggaaatgtta agcgagtagg tctcgatagt cgttcggcgg atcagaaaat catgaccgat
    32401 gtcttttttg gcgatagtga tgtggaacga gtcgatctct cttatcacga gagtttacaa
    32461 cgcattgtta aaggcgatgt cgatgcggtg atctggaacg tggtggcgga aaacgaactg
    32521 accatgctgg gattagaggc gacgccgcta acagacgatc cgcgattttt acaggccacc
    32581 gaagccgtgg tcctgacgcg agtcgatgat tacccaatgc aacaattgct gcgtgctgtt
    32641 gtagataaac acgccctgct tgcccatcaa caacgggtgg tgagtgggga acaggaaccc
    32701 agttattaaa cgaaaggcat ctgatatgga aaccagactc aacctgcttt gcgaggcagg
    32761 cgttattgat aaggacgtct gcaaaggcat gatgcaggtc gtcaacgtac tggaaaaaga
    32821 gtgccatctg ccggtgcgca gtgagcaagg aacgatggcg atgacacata tggcgagtgc
    32881 actgatgcgc agtcgccgtg gtgaagaaat agagccgctg gataacgagt tgctggcgga
    32941 actggcgcaa tccagccact ggcaagccgt tgtgcaattg catcaggagt tgttgaagga
    33001 attcgctctg gaagttaacc cgtgtgaaga aggctatttt ctggccaatc tttatggatt
    33061 atggatggct gctaacgaag aggtttgaag ctcccgacaa atccacgtcg cacaacctaa
    33121 atattcaaaa aaatgaatat ttaattcaag gaacagaaac gaggcaacaa gatgtttgta
    33181 gaagcattga aacgccagaa cccggcgctg atttccgccg cactaagttt gtggcagcag
    33241 ggcaaaatcg ccccggacag ctgggtgatc gacgtggatc agatactgga aaacggtaag
    33301 cggctcattg agacggcgcg gctttacggc attgaactgt atctgatgac caagcaattt
    33361 ggtcgtaatc cgtggctggc ggaaaaattg ctggcattag gctacagcgg cattgtggcg
    33421 gtggattaca aagaggcgcg agtcatgcgc cgcgctggtt tgccagtggc gcatcagggg
    33481 catctggtac aaatcccttg tcatcaggtt gctgacgccg ttgaacaggg caccgacatc
    33541 atcaccgtgt ttactctcga caaagcgcgg gaagtttctg cggcagcggt taaggccgga
    33601 cgggtccagc ctgtgctgct caaagtttat agcgacgatg attttcttta tccggggcag
    33661 gagagcggtt ttgtacagca ttcgctgcac gaggtgattg ctgaaattaa gaaactgccg
    33721 gggctgcatt tagccggact aacccatttc ccttgcctgc tttgggatga ggctgctggg
    33781 aaagttttgc caacaccgaa tcttcacacg ctggtacaag cacgggatca actggcgaaa
    33841 tctggtattg cgattgagca actgaacgta ccttcagcga ccagctgcac ttcgctgcca
    33901 ttgctggcgg aatacggtgc gactcatgcc gaacccggtc atgcgctgac cggtactatt
    33961 ccggcaaacc agcagggcga tcagcctgaa cgtatcgcga tgctctggtt aagtgaaatc
    34021 tcccatcatt tccgtggcga cagctactgc tacggcggcg gttactaccg tcgtggtcat
    34081 gcgcaacatg cgctggtgtt tacgccagaa aatcaaaaga ttactgaaac caatctcaaa
    34141 actgtggatg acagcagtat cgactacacc ctgccgctgg caggcgagta tccggtgagc
    34201 agtgcagtgg tgctctgttt tcgcacgcag atttttgtca cccgtagcga tgtggtgctg
    34261 gtgtccggta ttcagcgcgg cgaaccagaa atagttggtc gttatgacag cctcggcaac
    34321 cctctggagg cataaatggc gcgatttgtg gtgttagtga ttgatagctt tggcgtaggg
    34381 gcaatgaaag atgtcacgct ggtgcgcccg caagatgccg gggcgaatac ctgtggtcac
    34441 atcctgagcc agttgccgca tttgcagcta ccaacgctgg agaagctggg gctaatcaac
    34501 acattgggtt atgcgccagg cgatatgcag ccgtcagatt ccgcaacctg gggcgtggca
    34561 gagctgcaac atgaaggtgg cgataccttt atggggcatc aggaaatttt aggcacgcgc
    34621 ccgttaccgc cgctgcggat gccttttcgc gatgtgattg accgtgttga gcaggcatta
    34681 gtttccgctg gctggcaggt ggagcgccgt ggcgatgatc tgcaatttct gtcggtcaat
    34741 caggcggttg cgattggcga taatctcgag gcggatttag gccaagtcta taacattacc
    34801 gccaatctct ctgtgatctc ttttgacgac gcaatcaaaa ttggtcgtat cgtgcgtgac
    34861 caggtacagg tcggtcgggt cattacattt ggcggcctgt taaccaacag tcagcgtatt
    34921 ctcgatgccg cagaaagcaa agaagggcgc tttattggta ttaatgcgcc gcgttctggc
    34981 gcttatgaca acggcttcca ggtcgtgcat atgggctacg gtgttgatga aaaagtgcag
    35041 gtgccgcaaa aactgtatga agcgggcgtg ccaaccgtgc tggtgggtaa ggtggcagat
    35101 atcgtcagca atccttatgg tgtgagctgg caaaacctgg tggatagcca gcggattatg
    35161 gatatcaccc tcgacgaatt taacacccat ccgacggcgt ttatttgcat caatatccag
    35221 gaaaccgacc tcgctggtca tgcagaagac gtcgcacgtt atgccgaacg tttgcaggtc
    35281 gttgaccgta accttgcccg gcttgttgag gcgatgcagc cagatgattg cctggtcgtg
    35341 atggcggatc acggtaacga tccgaccatt ggtcacagcc accatacccg tgaagtggtg
    35401 ccagtgctgg tttatcagca agggctggtc gccacgcagc tcggtgtgcg caccacgctt
    35461 tctgatgtgg gagctaccgt gtgtgaattt ttccgtgcgc caccgccaca aaatggtcgc
    35521 tcttttcttt cctccctccg gtttgcagga gacaccctat gagttttgat ccgacgggtt
    35581 acaccctggc ccatgagcat ctgcatattg atctctccgg ctttaaaaac aacgtggact
    35641 gccgccttga tcagtatgcg ttcatttgcc aggagatgaa cgacctgatg acccggggtg
    35701 tacgtaatgt gattgagatg accaaccgtt acatggggcg caatgcgcaa tttatgctcg
    35761 atgtaatgcg cgaaaccggg atcaacgtgg tggcctgtac tggttattac caggatgcgt
    35821 ttttcccgga acatgtggtg acccgcagcg tgcaggaact ggcgcaggag atggtcgatg
    35881 aaattgaaca gggtatcgat ggcacggagc tgaaagccgg gatcatcgcg gagatcggct
    35941 ccagcgaagg aaagattacg ccgctggaag agaaggtatt tattgccgct gcgctggcgc
    36001 ataaccagac cggacgcccg atctccacgc atacgtcgtt cagcaccatg gggctggaac
    36061 aactggctct gttgcaagcc cacggagttg atctttcgcg cgtcaccgtt ggtcactgcg
    36121 atctgaaaga caacctcgac aacattttga agatgatcga tctcggcgcg tacgtgcagt
    36181 tcgacaccat tggtaagaac agttactacc cggacgaaaa gcgcattgcg atgctccacg
    36241 cgctacgtga ccgtgggttg ctgaaccgcg tcatgctgtc gatggatatt acgcgccgct
    36301 cccatttaaa agccaatggt ggttatggct atgactattt attgaccacc tttattccgc
    36361 aattgcgcca gtcaggattc agtcaggccg atgtggatgt gatgttacgt gaaaatccct
    36421 ctcaattttt ccaataagga cagactcatg aaaaagattg gcgttgcagg cttacagcgt
    36481 gagcagatta aaaaaaccat tgaagcgacg gctcctggct gttttgaagt tttcattcac
    36541 aacgacatgg aagcggcaat gaaggtgaaa tccgggcaac tagattatta cattggcgcg
    36601 tgtaataccg gtgcgggcgc ggcattgtcg attgccatcg cggtgattgg ctataacaaa
    36661 agttgcacca ttgccaaacc aggcattaaa gcgaaagacg agcatatcgc caaaatgatc
    36721 gctgaaggga aagtggcgtt tggcctttcc gttgagcacg tcgaacacgc gattccgatg
    36781 ttgattaacc atctgaaata aaaggcacga ctatggatct gtatattcag attatcgtgg
    36841 tggcgtgcct gacgggtatg acatcgcttc tggcgcatcg ctcggcggct gtttttcatg
    36901 acggcatcca cccgatcctg ccgcaactga ttgaaggcta tatgaaccgt cgcgaggcgg
    36961 ggagtatcgc ttttggtctg agcattggtt ttgtggcctc ggtggggatc tcttttaccc
    37021 tgaaaaccgg gctgctcaac gcatggttac tctttcttcc taccgatatc ctcggcgtac
    37081 tggcgataaa cagcctgatg gcgtttggtc ttggcgctat ctggggcgtg ttgatcctta
    37141 cttgcctgtt gccagttaac cagctgctga ccgcgctgcc ggtggatgta ttaggtagcc
    37201 tcggggaatt aagctcgccg gtggtttctg cttttgcact gttcccgctg gtggcgattt
    37261 tctaccagtt tggctggaag caaagtctgg tcgccgccgt ggttgttctg atgacccgtg
    37321 tggtagtcgt gcgctatttc ccacatctta accctgaatc catcgaaatc tttattggca
    37381 tggtgatgct gctggggatc gcgataactc acgacctgcg tcatcgtgat gaaaatgaca
    37441 tcgatgccag cgggctttcg gtgtttgaag agcgcacgtc gcggattatc aaaaacttac
    37501 cttatatcgc catcgtggga gcattgattg ccgccgttgc cagtatgaag atttttgctg
    37561 gtagcgaagt ttcgatcttc acactggaga aagcgtattc cgcaggcgtg acgccggaac
    37621 aatcgcaaac gctgattaat caggcggctc tggcagaatt tatgcgcgga ctggggtttg
    37681 tgccgttgat tgccaccacc gccttagcaa cgggtgtgta tgcagttgcg ggctttacct
    37741 ttgtttatgc ggtgggctat ctctcgccga atccgatggt tgcagcggta ttaggcgcag
    37801 tggttatttc ggcggaagtc ttgctgcttc gttcgatcgg caaatggctg ggacgctacc
    37861 cgtcggtgcg taatgcgtcg gataacatcc gtaacgccat gaatatgctg atggaagtgg
    37921 cgctgctggt cggttcgatt ttcgcagcaa ttaagatggc gggttatacc ggattctcta
    37981 tcgcggttgc catttacttc ctcaacgaat ccctgggccg tccggtacag aaaatggcgg
    38041 caccggtcgt ggcagtaatg atcaccggta ttctgctgaa tgttctttac tggcttggcc
    38101 tgttcgttcc ggcttaagga aggcacctat gaagacgttt cctttgcaaa gcctgactct
    38161 tgctgaagcg cagcaaaagc agtttgcgct ggtggacact atttgccgcc attttccagg
    38221 cagcgagttt cttgctggcg gtgatttagg gttaactcca agtctgaatc aaccgcgtat
    38281 tacccaacgg gtggagcagg tgctggctga tgcatttcac gcacaggccg cggcgctggt
    38341 gcagggcgcg gggactggcg cgattcgcgc cgggctggcg gctttgctca aaccggggca
    38401 gcgtcttctg gtgcatgacg cgcctgttta cccgacgaca cgggttatta ttgagcagat
    38461 ggggctgacg cttattactg ctgatttcaa tgacctgtcg gcactgaagc aggtcgtcga
    38521 cgagcaacaa cctgatgccg cgctggtgca gcatacgcgc cagcagccgc aggacagcta
    38581 tgtgctggca gatgtgctgg caacgttgcg cgcggcaggt gttccagcgt taaccgatga
    38641 taactatgcg gtgatgaagg tagcgcgaat cggctgtgaa tgcggcgcga atgtctcgac
    38701 attctcctgc ttcaagctgt ttgggccgga gggcgttggt gcagtggttg gcgatgccga
    38761 tgttattagt cggattcgcg ccacgcttta ctccggcggt agccagatcc agggctcaca
    38821 ggcgctggaa gtcttgcgtg gtctggtgtt cgcgccagtg atgcatgcgg tgcaggcggg
    38881 agtatccgaa cggttgctgg ctttgcttaa cggcggcgcg gtggcggaag tgaaaagcgc
    38941 cgtcattgcc aatgcgcagt cgaaggtgtt gattgtcgag tttcatcagc tgattgccgc
    39001 cagggtgctg gaagaagcgc aaaagcgtgg cgcgttgcct tacccggtcg gtgcagagtc
    39061 gaaatatgaa atcccgccgc tcttctatcg actttcagga acgtttcgcc aggcgaatcc
    39121 acaactcgaa cattgtgcga ttcgcattaa cccgaatcgc agcggtgaag agacggtgct
    39181 gcggattttg cgtgagagta ttgcgagtat ttaatgctgg atagcaccgt tcggcaatca
    39241 agctaaattc cgcactgaac ggtcccctcg cccctttggg gagagggtta gggtgagggg
    39301 aacgtgcggg atgtaaaatt cagtgcgcca gcatcgccgg atgcgatgct ggcgcatctt
    39361 atccggccta cggttaatgt cttttattat caatcgttaa ggtgatgcga ttggcgcggc
    39421 agaagagttc ggagacgtgc accggtttgt cattctgatc aaaggcgatt ttgctgatgc
    39481 gaaacaacgg ttcgcccagt tcgcatttca gccattttgc ctgcgggcgg gtggcggcaa
    39541 agatatcgat ggtctttttg tcgctgacca ctcgcgtgtc aaaacgctcc tgaaataact
    39601 gataggtgga gcttccttcg acgtaaatct catcaaagtc aggataacgc gacagcggga
    39661 tccatgaact atcaataaac agcggctctt tatcgagata catcacccgg cagagatgga
    39721 acacttcgct gtttccgggg atgttcagct tttcgcaaaa cggcgcggcg ctgacccgtt
    39781 cctgttcgat cactttctct ttcgtcgact tgccttgtga gacgccaaaa tcggtaaaac
    39841 cactgacagt aagcagggcg ttttcaactt tctggctttg tacaaaggtg ccttttccct
    39901 gccagcggat cagtacgccg tctgccacta agtcgctgat ggctttgcga atggtaatgc
    39961 ggctgacgtt atattgtgta caaagctcgt tttcggtagg gatctgttgc ccggcctggt
    40021 aaaccccctg cgcgatatca tccagcagcc gctggcggac ggtagcgtag aggagttgat
    40081 gagagtagcg gtccgtagct gacataatcg accctgaaat aaagaatgaa ttactcggat
    40141 tatagggtcg ttttccggca agtaacagca cgttggcgtg aagatgctca cgccaacgtt
    40201 atacctacca ggcaccgtgg tactgaatgg ttttcgccgc gcacgccgtt ccctgcgcca
    40261 tcgcctgcgg taatgtcatc cccgcagacc agccgcaaag gaatccggca atgaacgaat
    40321 ctccggcacc catggtgtcg ataaccgtca ccggttcagg agcctgacgc cagaactgcg
    40381 cgccatccta ggcaatgctg ccgttttcac ccagcgtgac aatcactgtt cctgcgccac
    40441 gggcaacaat cgctttcatc ttcagacgca gcgcttcgtc ttcttgcggt gcggaggcaa
    40501 aggcaaaatc gagatgcggc accagtgtct gccagagcgg gctgtcccac ttgtcggaga
    40561 agtcgaaagc ggtaagtttg cccgcagcgt gcagctgtgg gaatgcgtct tccgcatgtc
    40621 cccagattgc cgcgtgcaca atgtcatact gcgccagcca ggcgtaatcc tcttcactca
    40681 gggcaaagtc ggccatcacg ccttcggtgt agtcgccaaa aacgcgatca ttgtcgtgca
    40741 gttccacctg agtttgtgcg gtaacgccgt gtttcgtatg gacatggctg atatcgacgc
    40801 ccatgcgggc gagatcctgc ttcagctttg tgccgtagtc atcgtcaccc acccaggtaa
    40861 tgcatcccgg ctgtatgccg tagcgagtgc agtacaccgc cacattgacc gcattaccgc
    40921 cagaaaacgc tttattcagt tgcgggtaga tatcgacgca gttatcgccg attgtcgcca
    40981 gggttttcat ctctcatcct ccggcagcag cgcgcgaaag cgttccagcg cctggcgggc
    41041 atagagtctg ggctcgttca tatacatcgt caccagctcc accgtagagt aaccctcata
    41101 gccccgatca ataatatcgc gcatcagttc ccgcagcggc attttgcctt ctccaggaat
    41161 gtaatgcgtg tcgctggccc cgtcgctgtc gacaatatgc agatgacgta atttatcgcc
    41221 cagtttgtcg aaataactca tcaccggttc cgcctggaca tacggcgcgc aaatgtcgac
    41281 catgctgaac aggcgcggcg aaggcaccag cgccagcgca tgaagcacat cattagcatt
    41341 acacacgacg ttcgattcat acggcgttaa cggttcgagg atcagatcca tgccgatgtt
    41401 ttccgcgtac tcacacagtt cgcttaagtt ctctgccagc cgcccccaga taacgttagg
    41461 cggcgtgaga tagcccgcgt gggccgcaga aatcagcgta taacccgcgt tcatctcttt
    41521 tgccatatct atcgccagct tgatcatgtc caggctttca cgacgcatat gttcatcgcc
    41581 cagcatcatg ttatacggat agccgttggt ttctggcgtg taaccgataa tcggcatctg
    41641 atacgtctgc gccagcgcct tgatttgttt gatgccgccc gcttttaagt ccggcgcgaa
    41701 cgcgtgcggg cgaccgcccc aaatttcgat gccgtcgtaa cccagctcgc ttgcatcacg
    41761 aaatgcgtgt tcaataggca gccgctggtg gccgcaggta aacatacctg ttttcatcgg
    41821 gtaactcctt gcgtaaatcc ggcccgttgc cgggccggga gagggattaa tattccacca
    41881 gaccaccgta atagcggcgt tcatccgggt tgtgatcttt gtaaatagac aggtagtagc
    41941 agagccactc cattggcacg aacatcagga acggtgccag ccacgggtgc agcccttgcg
    42001 aaatttcggc gtaatcgatg acgatcacgt tgtcagtacg ctgtttaaca aagttaatgg
    42061 cgcgttcggt ggtgtggcga ctttcatcat tgccgagcag gaacaggaac ggaacgcccg
    42121 gttcgacaat ctccagcggg ccatggcgga actctccgct ctcaatcacg cagccgtgcg
    42181 tccaggtaaa ttccatcagc gttacaatgc cttctttgta acccagcgga cgcagcggac
    42241 ccgcagcaac ggtataaatc atcggccact ggctggccag ttcaccaagc tggcggcctt
    42301 tttcttccca ggtgcgtacc agatgaccga gcgcattcgg caactgcttg agatcgtttt
    42361 tgatcttgcc gatttccgcg ttcggcgcga ggcgggtgat catctccagc accacgctgt
    42421 agcagagcag caggtgaatt tcccagatac agtcggcctg ataatcaatg ctaaattccg
    42481 ccgccgaggt aatcgggctg tccgcgcgtt tggtgaacgc cgcagtgagt gctccgcagg
    42541 cccggcccag ctccagcgct ttgattacct cttccgtttt accgtagtca gaaacgccaa
    42601 tcaccgcgca acgatcgtcg aggcgatacg gggtgttatc gcagaactcc cagccggaaa
    42661 tggcgtagac ctgaagatcg gaaaagcgat ccgccagatg tttcgccgtt tgcgccgcgt
    42721 tgagtggcga gccgcaggca acgaaataaa tacgatcaat gtcgcgcttc accatctctt
    42781 ccacgatggc gtgcaccagc ggaacgtcat ggctgagaac tttttccact tcctgaacca
    42841 tattttcggt gaccagaaag tccacggtgc ttttatcaat atccaacatt ttattttctt
    42901 cagataacat tacgacaaag cgttgagctg gcggctacgc ttcgcccaga aggcgtaagc
    42961 aggcagacca gtagcaataa cgatggcggc gcagataagg ccgggaatcg gtgcccagac
    43021 aaaggttgag gcgaccagaa tgaggcttga cgcaatggcg agggtggtca tcagcccgaa
    43081 agccggagta cgccacagcg gtttgtaatc gtcgcgttta cgacaccaga tgatggagcc
    43141 gaaggtgagg gtatttttga aacacatcac cagggtgaaa taacccagca ggctggtgag
    43201 atcggaaacg aagatgaaga agatccccag cgccccttgc aggatgatgg agacatccgg
    43261 cgtgttgtat ttcggatgca catggccgaa gcatttaaag aacaggttgt ctttcgccat
    43321 cgcgtattcc agccgcggct ggtacatcac gcagctggaa agcgaaccaa gaatgacgat
    43381 catcgccgtg atggcaacaa agagcccagc ggtgctgccg agtgcgggga tctaggtcag
    43441 ggcgtcggaa atcggcgttt cagaattggc gagtttgtcg aagggcatca ggccggaaat
    43501 caccagcgcc agcagggtgt agagcaccag aaccagcaga caggaaccaa tcagcgctcg
    43561 tggcatggtt tttccggggt ttttaatttc gccggtcata taacagatag aggccatgcc
    43621 ggtatacgac caactggtgg cagagatccc cgccagcagc gccataaagc tgcccgttgc
    43681 gccaatcgca gtggtggtaa gggcggcaaa attctccgct ttaaaccaga agatcccaag
    43741 gccaatgacg atagtgaacg ggataatttt ggcgatggta attagcgtct gaaacgctgc
    43801 gccgccttca acggagcgca ggtgtagcaa cataaaggcg ataattaatc cggcggcgat
    43861 aaatttaccg agcaacggat cgataggcgt taaaaagcca agattgctga caatcgccag
    43921 cgccataatc gacaatgacg gcgcatcgtt ggcccagaag ctggcccagc cggagaggaa
    43981 agccagcggt cggcttccgg catttttcag ataaacataa tctgcgccat tttccggata
    44041 agcggtggat agttccgcat agacgcacat ttgcgggatc acaattaacc cgccaatgac
    44101 aaacgcgagc accgtaagcc acggcgtgcc cgctgctttt gccacttcac ccacagatac
    44161 aaaaataccg gagccgacgg ttgtcccgac ggcgattgca agaacggccc aaaatccgag
    44221 cttgcgttgg agttcctggc ttcccatagc gatacctttt tcttgtccaa tacgttgtag
    44281 gggagagttt gcagccattt aaaaggtaac atgacaatgc atgatgaata taacaacata
    44341 ttatgttatg tgatgtggag gaagatcaca aaatttcgca caggatcgcg ctgtggctaa
    44401 tggatgtagt tataaaattg aatttaaagt gaaaatattt ttacgggcgg gggcaagaag
    44461 gacatagaaa caaatacgcc ctcggaaaat ccagagggcg tcgggcaatt aaaccggtgt
    44521 tagccgattt ctgtcagaga cttactgtgc agtaggaccg caaccgccaa tgattttaga
    44581 aatagaaatt gccgggtgca gc
//
LOCUS       DAZSNO010000018        42552 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZSNO010000018 DAZSNO010000000
VERSION     DAZSNO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42552
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.018"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..798
                     /locus_tag="ACHWN2_000935"
     CDS             <1..798
                     /locus_tag="ACHWN2_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930573.1"
                     /translation="RSELSLINLLNSLNEVELMRKRLAFLMTAPEYACYNSQYCYEDK
                     VSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDIIISNTGEKFSEVGLTKYAQHL
                     ASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDTASANRVFHQHVPAFNTDHMIQ
                     YFINAKEWNNARELLIKEEMLGMHNLILLENICMQKNAECMAHITFTLNKLTTQPAIT
                     KIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYDRASGSASATQ"
     gene            complement(858..1349)
                     /gene="yajQ"
                     /locus_tag="ACHWN2_000936"
     CDS             complement(858..1349)
                     /gene="yajQ"
                     /locus_tag="ACHWN2_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR8930574.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1517..2428
                     /gene="panE"
                     /locus_tag="ACHWN2_000937"
     CDS             1517..2428
                     /gene="panE"
                     /locus_tag="ACHWN2_000937"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR8930575.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2391..2981
                     /gene="yajL"
                     /locus_tag="ACHWN2_000938"
     CDS             2391..2981
                     /gene="yajL"
                     /locus_tag="ACHWN2_000938"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR8930576.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3035..4483)
                     /gene="thiI"
                     /locus_tag="ACHWN2_000939"
     CDS             complement(3035..4483)
                     /gene="thiI"
                     /locus_tag="ACHWN2_000939"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR8930577.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQAAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4689..4931
                     /gene="xseB"
                     /locus_tag="ACHWN2_000940"
     CDS             4689..4931
                     /gene="xseB"
                     /locus_tag="ACHWN2_000940"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR8930578.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4931..5830
                     /gene="ispA"
                     /locus_tag="ACHWN2_000941"
     CDS             4931..5830
                     /gene="ispA"
                     /locus_tag="ACHWN2_000941"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR8930579.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5855..7717
                     /gene="dxs"
                     /locus_tag="ACHWN2_000942"
     CDS             5855..7717
                     /gene="dxs"
                     /locus_tag="ACHWN2_000942"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR8930580.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7772..8689
                     /gene="yajO"
                     /locus_tag="ACHWN2_000943"
     CDS             7772..8689
                     /gene="yajO"
                     /locus_tag="ACHWN2_000943"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR8930581.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(8800..9318)
                     /gene="pgpA"
                     /locus_tag="ACHWN2_000944"
     CDS             complement(8800..9318)
                     /gene="pgpA"
                     /locus_tag="ACHWN2_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR8930582.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9296..10273)
                     /gene="thiL"
                     /locus_tag="ACHWN2_000945"
     CDS             complement(9296..10273)
                     /gene="thiL"
                     /locus_tag="ACHWN2_000945"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR8930583.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(10351..10770)
                     /gene="nusB"
                     /locus_tag="ACHWN2_000946"
     CDS             complement(10351..10770)
                     /gene="nusB"
                     /locus_tag="ACHWN2_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR8930584.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10790..11260)
                     /gene="ribE"
                     /locus_tag="ACHWN2_000947"
     CDS             complement(10790..11260)
                     /gene="ribE"
                     /locus_tag="ACHWN2_000947"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR8930585.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11349..12452)
                     /gene="ribD"
                     /locus_tag="ACHWN2_000948"
     CDS             complement(11349..12452)
                     /gene="ribD"
                     /locus_tag="ACHWN2_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR8930586.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12456..12905)
                     /gene="nrdR"
                     /locus_tag="ACHWN2_000949"
     CDS             complement(12456..12905)
                     /gene="nrdR"
                     /locus_tag="ACHWN2_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR8930587.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13056..13595
                     /locus_tag="ACHWN2_000950"
     CDS             13056..13595
                     /locus_tag="ACHWN2_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR8930588.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            13893..14777
                     /gene="tsx"
                     /locus_tag="ACHWN2_000951"
     CDS             13893..14777
                     /gene="tsx"
                     /locus_tag="ACHWN2_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR8930589.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14954..15301)
                     /gene="yajD"
                     /locus_tag="ACHWN2_000952"
     CDS             complement(14954..15301)
                     /gene="yajD"
                     /locus_tag="ACHWN2_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR8930590.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15430..16401)
                     /gene="secF"
                     /locus_tag="ACHWN2_000953"
     CDS             complement(15430..16401)
                     /gene="secF"
                     /locus_tag="ACHWN2_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR8930591.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16412..18259)
                     /gene="secD"
                     /locus_tag="ACHWN2_000954"
     CDS             complement(16412..18259)
                     /gene="secD"
                     /locus_tag="ACHWN2_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR8930592.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18287..18619)
                     /gene="yajC"
                     /locus_tag="ACHWN2_000955"
     CDS             complement(18287..18619)
                     /gene="yajC"
                     /locus_tag="ACHWN2_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR8930593.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18642..19769)
                     /gene="tgt"
                     /locus_tag="ACHWN2_000956"
     CDS             complement(18642..19769)
                     /gene="tgt"
                     /locus_tag="ACHWN2_000956"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR8930594.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19824..20894)
                     /gene="queA"
                     /locus_tag="ACHWN2_000957"
     CDS             complement(19824..20894)
                     /gene="queA"
                     /locus_tag="ACHWN2_000957"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR8930595.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20987..21560
                     /gene="acpH"
                     /locus_tag="ACHWN2_000958"
                     /pseudo
     CDS             20987..21560
                     /gene="acpH"
                     /locus_tag="ACHWN2_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(21565..23382)
                     /gene="malZ"
                     /locus_tag="ACHWN2_000959"
     CDS             complement(21565..23382)
                     /gene="malZ"
                     /locus_tag="ACHWN2_000959"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR8930596.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23538..24911)
                     /gene="proY"
                     /locus_tag="ACHWN2_000960"
     CDS             complement(23538..24911)
                     /gene="proY"
                     /locus_tag="ACHWN2_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR8930597.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(24987..26306)
                     /gene="brnQ"
                     /locus_tag="ACHWN2_000961"
     CDS             complement(24987..26306)
                     /gene="brnQ"
                     /locus_tag="ACHWN2_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR8930598.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26713..28008)
                     /gene="phoR"
                     /locus_tag="ACHWN2_000962"
     CDS             complement(26713..28008)
                     /gene="phoR"
                     /locus_tag="ACHWN2_000962"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR8930599.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28066..28755)
                     /gene="phoB"
                     /locus_tag="ACHWN2_000963"
     CDS             complement(28066..28755)
                     /gene="phoB"
                     /locus_tag="ACHWN2_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR8930600.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28945..30147
                     /gene="sbcD"
                     /locus_tag="ACHWN2_000964"
     CDS             28945..30147
                     /gene="sbcD"
                     /locus_tag="ACHWN2_000964"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR8930601.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            30144..33287
                     /gene="sbcC"
                     /locus_tag="ACHWN2_000965"
     CDS             30144..33287
                     /gene="sbcC"
                     /locus_tag="ACHWN2_000965"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR8930602.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            33413..34594
                     /gene="araJ"
                     /locus_tag="ACHWN2_000966"
     CDS             33413..34594
                     /gene="araJ"
                     /locus_tag="ACHWN2_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR8930603.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(34737..35645)
                     /gene="mak"
                     /locus_tag="ACHWN2_000967"
     CDS             complement(34737..35645)
                     /gene="mak"
                     /locus_tag="ACHWN2_000967"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR8930604.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            35770..36681
                     /gene="rdgC"
                     /locus_tag="ACHWN2_000968"
     CDS             35770..36681
                     /gene="rdgC"
                     /locus_tag="ACHWN2_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR8930605.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(36759..37214)
                     /locus_tag="ACHWN2_000969"
     CDS             complement(36759..37214)
                     /locus_tag="ACHWN2_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930606.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37211..37546)
                     /locus_tag="ACHWN2_000970"
                     /pseudo
     CDS             complement(37211..37546)
                     /locus_tag="ACHWN2_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(37701..37985)
                     /gene="ppnP"
                     /locus_tag="ACHWN2_000971"
     CDS             complement(37701..37985)
                     /gene="ppnP"
                     /locus_tag="ACHWN2_000971"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR8930607.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38057..38734)
                     /locus_tag="ACHWN2_000972"
     CDS             complement(38057..38734)
                     /locus_tag="ACHWN2_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR8930608.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(38992..39183)
                     /gene="yaiA"
                     /locus_tag="ACHWN2_000973"
     CDS             complement(38992..39183)
                     /gene="yaiA"
                     /locus_tag="ACHWN2_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR8930609.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39233..39757)
                     /gene="aroL"
                     /locus_tag="ACHWN2_000974"
     CDS             complement(39233..39757)
                     /gene="aroL"
                     /locus_tag="ACHWN2_000974"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR8930610.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(39940..40398)
                     /locus_tag="ACHWN2_000975"
     CDS             complement(39940..40398)
                     /locus_tag="ACHWN2_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR8930611.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RECLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            40518..41327
                     /gene="proC"
                     /locus_tag="ACHWN2_000976"
     CDS             40518..41327
                     /gene="proC"
                     /locus_tag="ACHWN2_000976"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR8930612.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41344..42459)
                     /gene="adrA"
                     /locus_tag="ACHWN2_000977"
                     /gene_synonym="dgcC"
     CDS             complement(41344..42459)
                     /gene="adrA"
                     /locus_tag="ACHWN2_000977"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR8930613.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        10727 a        11361 c        10614 g         9850 t
ORIGIN      
        1 agaagtgaat taagcctcat taatttatta aattcattaa atgaggtgga attgatgaga
       61 aaacgtctgg cgtttctgat gacagcacca gaatacgctt gttataatag tcagtattgt
      121 tatgaagaca aagtcagcgc attaaaaatc ctgtatcgtc atcatgacaa tgagctaatg
      181 gacaaatatt tcacgactct tcttaaagag atcagtaatc aaccattaga agactgggat
      241 ataataataa gtaatactgg tgaaaaattt agtgaagtgg gtctaaccaa atatgctcaa
      301 catctcgcat ctttcgctga agaattttat ataaaagagc aaaaatcaca ttctgaaagt
      361 gaaatgagag gactattttc atcacttgcc gaattatata gttttggtaa tgatactgcc
      421 agcgcaaacc gagtgttcca tcagcatgtc cctgccttca atacagatca tatgattcaa
      481 tattttataa atgccaaaga atggaacaat gccagggaat tattgataaa agaggaaatg
      541 ttgggtatgc ataatctgat attgttggag aatatttgta tgcaaaaaaa tgcagaatgc
      601 atggcacaca ttacctttac gcttaataaa ttaacaacac aaccagcaat aacaaaaata
      661 gatagcgtgg gtaatgagca actttatcag attggcaaaa tctaccataa gttggaaatt
      721 aaacctgagc cagagcaaca ggttttaatt cagtcgcttt atgaccgggc atcaggaagc
      781 gcctctgcaa ctcaataaat gcatggtcaa aagggccacc acggtggccc tgacaatcag
      841 tagctatatc cagaaagtta atcgcggaag tttttgaact ggaacggctg accgagatcg
      901 ccaccgcgca ccatcgccat gacagcctgc aaatcatcac gagatttgcc agtcacgcgg
      961 atctcatcgc cctgaatttg cgcctgcact ttcagtttgc tgtctttgat catcttgacg
     1021 atttttttct gagtggcgct ctcaatgccc tgtttcagtt tcgcttccac aaaccaggtt
     1081 ttaccgctat gaacgatatt ttccggtaca tccagtgaac tgccttcaat gccgcgcttc
     1141 agcagcttgg cacgcagaat atccagcaac tgattgacct ggaagtcgga ctcgctcaac
     1201 actttgatgg ttttgctggc gtcgttcagc tcaaatgagg cttcaacgtt acggaagtca
     1261 aaacgggact ccacttcgcg actcgcgtta tcgaccgcgt tacgtgcttc ctgaagatca
     1321 acttcagaga caatatcgaa agatggcatc ttttcctctc ccttcatttt tgatgcgaag
     1381 cataatacct gcaaagttaa ataaccaaca gtcctgcacc aggacgtatc ccttaattgt
     1441 gcatagtcgc aacagcatgc acaattaagg gatagcctta ttgaacaccc ggagtggttg
     1501 cgggtgagga ggaacaatga aaattaccgt attgggatgc ggtgccttag ggcaattatg
     1561 gcttacagca ctttgtaaac agggtcatga agttcagggc tggctgcgtg taccgcaacc
     1621 ttattgtagt gtgaatctgg ttgagacaga tggttcgata tttaacgaat cgctgaccgc
     1681 caacgatccc gattttctcg ccaccagcga tctgctcctg gtgacgctga aagcatggca
     1741 ggtttccgat gccgtcaaaa gcctcgcatc cacactgcct gtaactacgc caatactgtt
     1801 aattcacaac ggcatgggca ccatcgaaga attgcaaaac attcagcagc cattactgat
     1861 gggcaccacc acccatgccg cccgccgcga cggcaatgtc attattcatg tggcaaacgg
     1921 aatcacgcat atcggtccgg cacggcaaca ggatggcgat tacagttatc tggcggatat
     1981 tttgcaaacc gtgttgcctg acgttgcctg gcataacaat attcgcgccg agctgtggcg
     2041 caagctggcc gtcaactgcg tgattaatcc actgactgcc atctggaatt gcccgaacgg
     2101 tgaattacgt catcatccgc aagaaattat gcaaatatgc gaagaagtcg cggcagtgat
     2161 cgaacgcgaa gggcatcata cttcagcgga agatttgcgt gattacgtga tgcaggtgat
     2221 tgatgccaca gcagaaaata tctcgtcgat gttgcaggat atccgcgcgc tgcgccacac
     2281 cgaaatcgac tatatcaatg gttttctctt acgccgcgcc cgcgcgcatg ggattgccgt
     2341 accggaaaac acccgcctgt ttgaaatggt aaaaagaaag gagagtgaat atgagcgcat
     2401 cggcactggt ttgcctcgcc cctggtagtg aagagactga agccgtcacc acgatcaacc
     2461 tcttggttcg cggcggtatc aaagtcacca ctgccagcgt cgacagcgat ggcaacctgg
     2521 caattacctg ctcgcgcggc gtgaagctgc tggcggatgc gccgctggtc gaagtggctg
     2581 atggcgaata tgacgtgatc gtgctgcctg gtggcattaa aggcacggag tgttttcgcg
     2641 atagcactct gctggttgaa accgttaaac agttccaccg ttccgggcgt atcgtcgcgg
     2701 ctatttgcgc cgcgccagcc accgtgctgg tgccgcacga tatctttccg attggcaata
     2761 tgaccggctt cccgacgctg aaagacaaaa ttcccgctga acaatggcag gacaagcgcg
     2821 tcgtctggga tgcacgggta aaattgctga ccagccaggg gccgggtaca gctatcgact
     2881 ttggtctgaa aattatcgac ctgctggttg ggcgcgagaa agcccatgaa gtggcatcac
     2941 aactggtgat ggcggcaggg atttataatt attacgagta gtgtcggatg cggcaaacgt
     3001 cgcatctgac cagatgcgac gtaaaaacta caaattacgg gcgatatacc ttcacattgt
     3061 taaagccttg ctcgcgcaga tagagcgcct gcagacggct catcaccccg cgctcacacc
     3121 acagcagcca ggttttgttc tggtcgagat cgccaaattt ggtgctcagt ttatagaacg
     3181 gcagagaaac cacatcaatc ccttcgactt tcagtggctt atcttcctgt tcatcgacag
     3241 aacggatatc gagaatcacg tcgttcgggc cgaagccgtt gacggtttcc acttccacca
     3301 cttcctgctc ggtctgctgg gcgatttcgc ggatatcaac gttattcgct tcctcaacca
     3361 ctttatcgag aatactgaag tcgaacttct cttcttccgc ttcaatcttc gatttaaccg
     3421 ctttcaccgt cgggcttttg gagatcacgc cacaatattc cggcatcgtg cgagcaaagt
     3481 cttcggtgcc aatctggcga gccaggttga tgatgtgctc tttgtcgtaa gaaatcagcg
     3541 gacgcaggat cagcgtgtca gagacgttat caatcaggcg caggttggtc agcgtctgac
     3601 tggacacctg gccgagcgct tcgccggtga ccagcgcctg tacgccgtaa cgttcagcca
     3661 ctttagatgc ggcacgcacc atcatacgtt tgaggataac gcccatctga ccgtcgtcga
     3721 ttttctcgag aatttccccg acaaccggtt cgaaattaat ggcgacaaaa cgcacgcggt
     3781 gggagctgcc aaagcggttc cacagataat gcgccgcctg acgcacgcca atttcatgcg
     3841 ctgcgccgcc gaggttaaag aagcagtaat gcacgcggca gccgcgacgc atcaacatat
     3901 aactggaaac accggagtcg aaaccaccgg aaatgagcga cagcacatct tcctgggtgc
     3961 caatcgggaa accaccaata ccttcgtagc gacctttaat cagcaggagg cgatcgtctt
     4021 ccacttccag atgaacagtt acatccgggt tagtcagctt cacgcgcgcg gattcaatat
     4081 gctgatttaa accgccgccg acgtaacgtt ccacatcaat cgagctaaaa tcatgtttgc
     4141 cacggcgctt cacgcgtacg cagaaggttt tgccttccag ctgatcgcga tactgaacca
     4201 acgctttctc aaaaatatcg tgcatgtcgg taaacggcac gtcttcgact tcgagaatat
     4261 ggtggatacc cggaatacgg gtcagggcgt cgcgaatagt cagacgctgg ttttcatctt
     4321 tcgcgcgaac ttcgatgtta tcccagtggc ggacgacagc gagcgtctca tcatagtgct
     4381 ttaaaacgtt acgaatgttc ccggtaagga tttttataaa gcgcaagcgc acagattggc
     4441 ttttgatggt gatttccggg aacaatttaa tgataaactt catggcggca atggttcgtt
     4501 ggcaagcctt aagcgacttg tatagggaaa aatacagcag cccacacctg cggctgcatc
     4561 caggcgcgga agtataccac taacatcgct ttgctgcgca catcacctta ccattgcgcg
     4621 ttatttgcta tttgccctga gtccgttacc atgacggggc gaaaaatatt gagagtcaga
     4681 cattcattat gccgaagaaa aatgaggcgc ccgccagctt tgaaaaggcg ctgagcgagc
     4741 tggaacagat tgtaacccgt ctggaaagtg gcgacctgcc gctggaagag gcgctgaacg
     4801 agttcgaacg cggcgtgcag ctggcacgtc aggggcaggc caaattacaa caagccgaac
     4861 agcgcgtaca aattctgctg tctgacaatg aagacgcctc tctaacccct tttacaccgg
     4921 acaatgagta atggactttc cgcagcaact cgaagcctgc gttaagcagg ccaaccaggc
     4981 gctgagccgt tttatcgccc cactgccctt tcagaacact cccgtggtcg aaaccatgca
     5041 gtatggcgca ttattaggtg gtaagcgcct gcgacctttc ctggtttatg ccaccggtca
     5101 tatgtttggc gttagcacaa acacgctgga cgcacccgct gctgccgtag agtgtatcca
     5161 cgcttactca ttaattcatg atgatttacc ggcgatggat gatgacgatc tgcgtcgcgg
     5221 tttgccgacc tgccatgtga agtttggcga agcaaacgcg attctcgctg gcgacgcttt
     5281 acaaacgctg gcgttctcga ttctaagcga tgccaatatg ccggaagtgt cggatcgcga
     5341 cagaatttcg atgatttctg aactggcgag cgccagcggt attgccggaa tgtgcggtgg
     5401 tcaggcatta gatttagacg cggaaggcaa acacgtacct ctggacgcgc ttgagcgtat
     5461 tcatcgtcat aaaaccggcg cattgattcg cgccgccgtt cgccttggtg cattaagcgc
     5521 cggagataaa gggcgtcgtg ctctgccagt actcgacaag tacgcagaga gcatcggcct
     5581 tgccttccag gttcaggatg acatcctgga tgtggtagga gatactgcaa cgttgggaaa
     5641 acgccagggt gccgaccagc aacttggtaa aagtacctac cctgcacttc tgggtcttga
     5701 gcaagcccgg aagaaagccc gggatctgat cgacgatgcc cgtcaggcgc tgaaacaact
     5761 ggctgaacag tcactcgata cctcggcact ggaagcgcta gcggactaca tcatccagcg
     5821 taataaataa acaataagta ttaataggcc cctgatgagt tttgatattg ccaaataccc
     5881 gaccctggca ctggtcgact ccacccagga gttacgactg ttgccgaaag agagtttacc
     5941 gaaactctgc gacgaactgc gccgctattt actcgacagc gtgagccgtt ccagcgggca
     6001 cttcgcctcc gggctgggca cggtcgaact gaccgtggcg ctgcactatg tctacaacac
     6061 cccatttgac caattgattt gggatgtggg acatcaggct tatccgcata aaattttgac
     6121 cggacgccgc gacaaaatcg gcaccatccg tcagaaaggc ggtctgcacc cgttcccgtg
     6181 gcgcggcgaa agcgaatatg acgtattaag cgtcgggcat tcatcaacct ccatcagtgc
     6241 cggaattggt attgcggttg ctgccgagaa agaaggcaaa aatcgccgca ccgtctgtgt
     6301 gattggcgac ggcgcgatta ccgctggcat ggcgtttgaa gcgatgaatc acgcgggcga
     6361 tattcgtcct gatatgctgg tggtcctcaa cgacaatgaa atgtcgattt ccgaaaatgt
     6421 tggcgcgctt aacaaccatc tggctcagct gctttccggt aagctttact cttcactgcg
     6481 cgaaggcggg aaaaaagttt tctctggcgt gccgcccatt aaagagctgc tcaaacgtac
     6541 cgaagaacat attaaaggca tggtggtgcc tggcacgttg tttgaagagc tgggatttaa
     6601 ctacatcggc ccggtggacg gtcacgatgt gctggggctt atcaccacgc taaagaacat
     6661 gcgcgacctg aaaggcccgc agttcctgca tatcatgacc aaaaaaggtc gtggttatga
     6721 accggcagaa aaagacccga tcactttcca cgccgtgcct aaatttgatc cctccagcgg
     6781 ttgtttgccg aaaagtagcg gcggtttgcc gagctattca aaaatctttg gcgactggtt
     6841 gtgcgaaacg gcagcgaaag acaacaagct gatggcgatt actccggcga tgcgtgaagg
     6901 ttccggcatg gtcgagtttt cacgtaaatt cccggatcgt tacttcgacg tggctattgc
     6961 cgagcaacac gcagtgacct ttgccgccgg tctggcgatt ggtggataca aacccattgt
     7021 cgcgatctac tccaccttcc tgcaacgcgc ctatgatcag gtgctgcatg acgtggcaat
     7081 tcaaaagcta ccggtcctgt tcgccattga ccgcgcgggc attgttggtg ctgacggtca
     7141 aactcaccag ggcgcttttg atctctctta cctgcgctgc ataccagaaa tggtcattat
     7201 gaccccgagc gatgaaaacg aatgtcgcca gatgctctat accggctatc actataacga
     7261 tggcccgtcc gcggtgcgct acccgcgcgg caacgcggtt ggcgtggaac tgacgccgct
     7321 ggaaaaacta ccaattggca aaggcattgt gaagcgtcgt ggcgagaaac tggtgatcct
     7381 taactttggt acgctgatgc cagaaacggc caaagtcgcc gaatcgctga acgctacgct
     7441 ggtcgatatg cgttttgtga aaccacttga tgaagcgtta attctggaaa tggccgccag
     7501 ccatgaagcg ctggtcaccg tagaagaaaa cgccattatg ggcggcgcag gtagcggagt
     7561 gaacgaagtg ctgatggccc atcgtaaacc agtacccgtg ctgaacattg gcctgccgga
     7621 cttctttatt ccgcaaggaa ctcaggaaga aatgcgcgcc gaactcggcc tcgatgccgc
     7681 cggtatggaa gccaaaatca aggcctggct ggcataatcc ctactccact cctgctatgc
     7741 ttaagaaatt attcatggca ggagtggcag catgcaatac aaccccttag gaaaaaccga
     7801 ccttcgcgtt tcccgacttt gcctcggctg tatgaccttt ggcgagccag atcgcggtaa
     7861 tcacgcatgg acactgccgg aagagagcag ccgtcccata attaaacgcg cgctggaagg
     7921 tggcataaat ttctttgata ccgccaatag ctattccgat ggtagcagcg aagagatcgt
     7981 tggtcgcgca ctgcgggatt tcgcccgtcg tgaagacgta gtcgttgcca ccaaagtgtt
     8041 ccatcgcgtt ggtgatttac cggaaggatt atcccgtgca caaattttgc gctctatcaa
     8101 cgacagcctg cgacgtctcg gcatggatta tgtcgatatc ctgcaaattc atcgctggga
     8161 ttacaacacg ccgatcgaag agacgctgga agccctgaac gacgtggtaa aagccgggaa
     8221 agcgcgttat atcggcgcgt catccatgca cgcttcgcag tttgctcagg cgctggaact
     8281 acaaaaacag cactgctggg cgcagtttgt cagtatgcag gatcactata atctgattta
     8341 tcgcgaagaa gagcgcgaga tgctgcctct gtgttatcag gagggcgtgg cggtgattcc
     8401 gtggagcccg ctggcgcggg ggcgactgac gcgtccatgg ggagaaacta ccgcacgact
     8461 ggtgtctgat gaggtgggga aaaatctcta taaagaaagc gatgaaaatg acgcgcagat
     8521 cgcagagcgg ttaacgggcg tcagtgaaga actcggtgca acacgagcac aagttgcgct
     8581 ggcctggttg ttaagtaaac cgggcattgc cgcaccgatt atcggaactt cgcgggaaga
     8641 acagcttgat gagctgctga acgcggtgga tatcactttg aagccataac agattgccga
     8701 gctggaaacg ccgtataaac cgcatgcggt cgtaggattt aaataagagt ccaggcctga
     8761 tgagacgtga caagcgtcac atcaggcatc ggtgcacaac tacgacagaa tacccagcgg
     8821 ccagtgatga ccgataaagt acaggatgcc tgcggaaatc accccggcga caatatcgtc
     8881 gatcatgatc cccatgccgc catgtacatt gcgatcaaac cagcggatcg gccacggctt
     8941 ccacatatcc agaatacgga aaatcacaaa cccggcggca acccactgcc agtcattggt
     9001 cggcagcgcc atgagcgtga tccacatacc aataaattcg tcccagacaa tgctgccgtg
     9061 atcgtgcacg cccatatctt tcgccgtttg atgacaaaga tagacgccga tacagatccc
     9121 caacatcacc accagcgagt agagctgcca gggtaaaaag gtcatcagat accagaaagg
     9181 gatcgccgcc agcgatccca tcgagccagg aacgattggg cttaatccac ttccgaatcc
     9241 gacagcaagc agatgccacg gattactcat cttcaggcga cttttcgcga catctttatg
     9301 gcgtgacaaa atggtcatat cctttccagt caaacgtgac aggttcgccg tcacgaataa
     9361 aacaaagccc ttcgatatcg gcggtcattt gcccgataca ggtaaacggt acgccaaggt
     9421 gtccgagagc aacatccagc gcgccacggt tcagttccgg caccgtgaaa cacaactcgt
     9481 aatcttcacc gccagagagc gcccagcgca gcgcctgttc cggttcaaca tggcgagaaa
     9541 gcgcatcaga aaacggcagc aatgccaggt caatacgtgc gccgcagtcg ctggctttca
     9601 cgatatgccc gagatcggaa atcagaccgt cagagagatc gatagctgaa tttgccagat
     9661 cgcgcagtgc ctgcccctgt aaaatacgcg gcgatggacg gagatgacgt ttgatcaagt
     9721 agtcagcatc tttagcatcg gcaacctgca aacggttttg caaaatcgcc agcccggcgg
     9781 cgctatcgcc cggtgtaccg gtcacataga tccagtcacc cggtttcgcc ccagagcgcg
     9841 ttaaggctcg tcccatcgga acaaagccgt ggatacccaa cgtcattgat aatggcccac
     9901 gcgtggtatc gccgccaatg agttgcatat cgtaataatt gagaagatca aacaaactgt
     9961 cgctgaagga ctcaagccac gcttcgtcta cgtccggtaa ggttaatgcc agcgtcagcc
    10021 aggccggatc ggcccccatc gctgccagat cgcttaggtt caccgccagt gctttataag
    10081 ccagatcagc aggatcgata tcagggagga aatggttgcc cgccaccagc gtatcagtgc
    10141 tgatcgccag ggtctgtttc tcggggatat tgagaagagc gcaatcgtcg ccgatgccca
    10201 gttcgacatc aagacgagaa cttcttacac ggtcaaaata acgggcaatc agggagaact
    10261 cgccacatgc catacgttat gcctcagcag gtaaaaaaga aaaggccggc gatcatggaa
    10321 cagtcttccg tgaatctacc ggcctggata ttactttttg ttagggcgaa tcacaggtgc
    10381 tgctttatcg agtacgccgt tgacgaactt atggctgtct tctgcgccga acgatttcgc
    10441 cagttcgatc gcttcgttaa tggccacttt gtatggcaca tcactacgtt tagacagttc
    10501 gtacagcgca atgcgcagta ctgctttttc tacctggccc agttcttcca gcaggcggga
    10561 caggtagggc ttcatcagtc cgtcgaggta tgcggtgtta gtcgccaccc cggccagcag
    10621 ctcacggaag tacaggacgt caacgtcttt tacatcctgt tcagccagga actggtattc
    10681 aacatcagcg atgtcgttct gggacaactg ccaggagtag agcgcctgga cggcacactc
    10741 acgagcgcgg cgacgagcag caggtttcac ggatttcccc ttactaattt caggccttga
    10801 tggctttcaa tacattaatc atttcaagcg cggtcagtgc agcttctgca cctttgttgc
    10861 cagctttggt gccagcacgt tcgatcgctt gttcaatgct ttcagtggtc agaaccccaa
    10921 aagcaaccgg aatttcgctg tcctgggcaa catgcgccag gccgttgctt gcaccaccag
    10981 cgacatattc aaagtgggca gtgccaccac gaataaccgt acccagcgca atcaccgcgt
    11041 cgtatttacc ggttttagcc agtgcacccg ccgccagcgg cagctcatag gcaccaggca
    11101 cccaaacaac ggtaatgttt tcatctttta cctgaccgat acgtttcagt gcgtcaattg
    11161 caccttccag caggctgtca ttgataaagt tgttgaaacg cgcgatggtg atggcgacgc
    11221 gagcgtccgg ggtagcaacg ttagcttcaa taatgttcat tttcttcctt cgggttcgag
    11281 tatggccccg caggggggcg gattttagca taatatttcg tgcgctgctt ccctttcgag
    11341 ccgggagatc atgcacccac taaatgcagg caaacatccg ggcctacatg acgtatctct
    11401 ttgaatttaa attggggggc gtcggctaat ttctcaagcc ctggcagcga gcataatcca
    11461 cgggcgtcgc tgcctaatag tttaggtgcg atatagacaa tcagctcatc gactaaaccc
    11521 gcctgtagca atacgccagc gagcgttggc cccgcttcca cccagatgct gttaatctgc
    11581 tgtttaccca gttgcatcat cagtacaacc agatccagat gacctttatg ctctggaatc
    11641 agcaaggtac gcaccgtttc cggccactca cgagaatctt cctgcgtacg cgcgaaccag
    11701 gtttcgccgg gctgctgcac aatgcgatgt tccggcgtca cgcgattttg gctatcaatc
    11761 acaatacgta ctggctggcg gagattttgt tgcgggtaga gcgcctgagt ttgttcatcc
    11821 agttcagacc aacgcactgt taaggctgga tcatccgcca gcaccgtggc gctgctggtt
    11881 aaaatggcat gactttgtgc gcgctgtcgt tgtacgtcgc gccgcgcctg aggcgaagtg
    11941 atccactggc tttcgccgct cgccatcgcc gtgcggccat caagcgatgc gccaagtttt
    12001 aactgaatat aaggaaagcc ggtgcgcatc cgcttgagaa agcctttatt caattgctcg
    12061 gcttcactca tcatcagccc gtggctgacg tcaatgccag cctgttgcag acggtaaagt
    12121 ccacgcccag cgacctgcgg gttaggatct tgcatcgctg cgaccacgcg cgctacgcca
    12181 gcggcgatga gtgcgtcaca gcacggtggc gtacggccat gatggctaca gggttcgagt
    12241 gtcacatacg ccgttgcgcc tttggctttt tcacccgcca tacgcaacgc gtgtacttcg
    12301 gcatgtggtt cacccgctcg ttggtggtaa ccttcaccga caatttcgcc atctttgaca
    12361 atgacgcacc cgacattcgg gttggggtgg gtggtaaaac gtcctcgttg cgccagcttt
    12421 agcgcccgcg ccatgtaata ctcgtcctgc acggcttagt cctccaggcg cgcgatctct
    12481 tcgccaaatt ctttgatatc ttcgaaactg cggtagacag aggcaaaacg gatataggcg
    12541 actttatcga gctttttcaa ttgctccatc accagattgc caatcatctt gctcggtact
    12601 tcgcgctcac cggtggcgcg cagctgcgat ttaatatgat tgattgccat ttcaacgtaa
    12661 tcggaactca ccggacgttt ttccagcgcc cgcagcattc cgctacgcaa tttctcttca
    12721 ttaaacggtt cacgcacgtc gttgcttttt acaacacgcg gcataaccag ctccgccact
    12781 tcaaaggtgg tgaaacgttc attacacacc agacactgcc gacggcggcg tacggatgaa
    12841 ccctcgccca cgagacgaga gtcaattacc ttagtgtcca cggcgaaaca gaatgggcaa
    12901 tgcatacggc gtcctgacca ggtggttaac ggaaatctat tttaccctga actgacgata
    12961 caacaaaggc gcaacgcttt tgagataaag gatcgatgct ttcgctgctt ttaccctcta
    13021 ccattaggct aattgcgacg agaaagggac gacctatgaa tactaacgtt tttcgactgc
    13081 tcctgctggg aagcctgttc agccttagcg cctgtgtgca gcaaagtgaa gtgcgacaga
    13141 tgaaacacag cgtcagcacg ctgaaccagg agatgacgca actcaatcaa gaaacagtca
    13201 aaatcactca gcaaaacagg ctgaatgcaa aatccagcag tggggtttac cttctgcctg
    13261 gagcgaaaac accggcaaga ctggaaagcc agatcggtac tttacgtatg tcgctggtga
    13321 atattacgcc tgatacagat ggcaccaccc ttacactacg tattcagggt gagtcgaatg
    13381 atcccttgcc cgcattcagc ggaactgttg aatatgggca aattcaggga acaatagaca
    13441 actttcagga aatcaatgtg cagaatcaat tgattaatgc ccctgccagc gtcctcgccc
    13501 ccagcgatgt tgatattccg ttacaattaa aggggatttc tgtggatcag ttaggctttg
    13561 tacgtatcca cgacattcaa cctgttatgc agtaaacgta tttcgggacg attttgtgcg
    13621 tcccgcaaca tcttccccgt cattttgtta ctctgcttac atcacctgga ttgatagtaa
    13681 aagtttgcaa caagggcgaa agtcagtaca atccccgccc gaatgtgtgt aaacgtgaac
    13741 gcaatcgatt acgtaaatga tagaactgtg aaacgaaaca tatttttgtg agcaatgatt
    13801 tttataatag gctcctctgt atacgaaata tttagaaacg caatttgcgc ctttttcact
    13861 cccgcaaggg attttcaaac agtggcatac atatgaaaaa aacattactg gcagccggtg
    13921 cggtactggc gctctcttcg tcttttactg tcaacgcagc tgaaaacgac aaaccgcagt
    13981 atctttccga ctggtggcac cagagcgtta acgttgtcgg aagctatcac acccgtttcg
    14041 gaccgcagat ccgcaacgat acctaccttg agtacgaagc attcgctaaa aaagactggt
    14101 tcgacttcta tggttatgcg gatgcgccgg tattcttcgg cggtaactcc gatgctaaag
    14161 gtatctggaa ccacggttct ccgctgttta tggaaatcga accacgtttc tccatcgaca
    14221 agctgaccaa tactgacctt agcttcggtc cgttcaaaga gtggtacttc gcgaacaact
    14281 acatttacga catgggtcgt aataaagatg gtcgccagag cacctggtac atgggtctgg
    14341 gtaccgatat cgacactggc ctgccgatga gcctgtccat gaacgtctat gcgaaatacc
    14401 agtggcagaa ctatggcgca gcgaacgaaa acgagtggga cggttaccgt ttcaaaatta
    14461 aatactttgt gccgattacc gatctgtggg gcggtcagct gagctacatc ggcttcacca
    14521 acttcgactg gggttccgat ttaggggatg acagcggtaa cgcaatcaac ggtattaaga
    14581 cccgtactaa taactctatc gcttccagcc atattctggc tctgaactac gatcactggc
    14641 actactctgt cgtagctcgt tactggcacg acggtggtca gtggaacgac gatgcagaac
    14701 tgaacttcgg caacggcaac ttcaacgttc gctctaccgg ctggggtggt tacctggtag
    14761 taggttacaa cttctgatta tgaaaatgcc gggatttatt cccggcattt ctgattgtta
    14821 acaacgtgtg ctttgttcat gccggatgcg gcgtaaacgc tttgtccggc ccacaaaagc
    14881 acgtaaattc aatatattgc tgaaataacg taggcctgat aagcgtagcg catcaggcaa
    14941 ttttacgttt taatcacttc ttcttgttca tcatcgcttt caggtcagcg aatgggttgt
    15001 acttcgcttc accgacatct ttctgcgcat cttcccctgc gataacggtc gtaccatact
    15061 gatccgcttc ggtatatttc gaatgctcat gatcgtggca atagagacac aacaattccc
    15121 agttactgcc atcttccggg ttattggtat ggtcgtgatc aatgtggtga acggtaagtt
    15181 cacgcaggtt agaataaaca aactcgcggg aacagcgacc gcagacccac ggatagattt
    15241 ttaatgcttt ttcgcgatag ccgctttcta accgtgcata gttttttggg atgatagcca
    15301 tgaaaagtta cctggatgaa atgtagggtt acgcgcagtt tcccaagatg cggggatttt
    15361 cgcaatctcc atacatcaaa aaaaatcccg atcttctgac cgggattttc aacatcaacg
    15421 ggaacttgat taaggcagaa ttgacggctg atccgcccct tctttttcca ctttctgctg
    15481 caacatgtgt tcgcgcttca tacccagttt cagagccaac gcagatgcta cgtagatgga
    15541 agatgcagtg ccgatggaaa caccgataag catggtcagc gagaagcctt ccagtaccgg
    15601 accaccgaag agatacagca tcaggataac catcaaggta gtaccggatg tgatcaaggt
    15661 acggtgcagc gtctgggtca aggacacgtt aaagatttcg taaggcgtac cgcgacggat
    15721 cttgcggaag ttttcacgaa ttcggtccga taccacgata ctgtcgttaa gcgagtaacc
    15781 gataaccgac atcaacgatg ccacaatggt caggtcaatc tcgatatgga ataacgacaa
    15841 aatacccagc gtaatgataa cgtcgtgcgt cagcgcaata accacccctg ccgccagtcg
    15901 ccactcaaag cggaaaccta cgtacacgag gatagacagc agcgctgcca tcaacgccat
    15961 cgcaccggtt tgcgcaaggt cagcccccac gctcggaccg acgaactcaa tacgcttcac
    16021 tgctgcattc tgattggtgg attcgttaat caccttcaga acttggctgc ccagcacctg
    16081 accgccggtt tcgccttcag caggcggcat acggaccatg atgtcgtggc tgctaccaaa
    16141 gttttgcagc attggctctt caaaaccggc tttttgcaat gcatcacgca ttacgtcaat
    16201 ttcagccggt ttttcgagcg taatttcgat aaccgtacca ccggtgaaat ccagccccca
    16261 gttaaagccg cgcacgccca taataacgat agcagcgatt aacagcagac cagagatgcc
    16321 gaaagcccag tagtcccagc gcataaagtc atagacttta cggccgtggt ttagttgttc
    16381 aacagtatat tcctgtgcca catcgcactc ctcagattga cagcttcttg acgcgcttgc
    16441 cgccatatag caggtttacg atggcacgcg taccgacaat cgcggtaaac atcgacgtcg
    16501 ccacaccgat accggtagta atcgcgaacc ctttaattgc cccggtaccc actgcgtaca
    16561 ggatgatgac tttaatcagc gtggtgatgt tcgcatcgaa gatagaactg aatgcgccac
    16621 gataaccttc atcaattgcc tgttgaacag tacgtccgtt gctcaactct tctttaatac
    16681 gttcgttgat cagtacgttc gcatcgaccg ctaccgcaag ggttaagacg atacccgcga
    16741 taccaggcat actcagtgtt gcgcctggca acagcgacat aataccgacg atcaagatca
    16801 agttggcaat cagagcactg gtcgcaatca gaccaaactt cttatagaag atgatcatga
    16861 acagaataga aaccagcaga ccggcaaggc aagcctccag cccctgctca atgttctgca
    16921 tacccagggt cgggccaatt gtacgttctt caacaatctg aatcggcgcg atcaacgcac
    16981 cggcacgcag cagcagtgac agctgacggg cttcgttcgg gttgttgatg ccggtgatac
    17041 ggaagctgtt acccagacga gactggatgt tggcgatgtt aatcacctct tcctgtttca
    17101 ccagaaccgc acgaccattt gcatctttct taccgctgtc tttgtactcc acaaacaggg
    17161 ttgccatcgg tttgccgatg ttgtccttgg tgaagttaga cataatgttg ccaccagcgc
    17221 tatcgagcga gatgttaacc tgcggctggt tgtattcgtc ctggctggaa gtggagtcgg
    17281 tgatatggtc accagtcaga attacgcgtt tgtacagcac aaccggctga ccttcgcggg
    17341 tctgtttcac ttcagagtcg cccggtacgc gaccggatgc cgcagcggcc tgatcaacat
    17401 tggtgtttac cagacggaat tccagcgttg ccgtcgcgcc cagaatctct ttcgcacgcg
    17461 cagtgtcctg aataccgggc agttcaacaa cgatacggtc agcaccctga cgctgaacca
    17521 ccggctccgc cacgccaagt tggtttacac ggttacgcag gatattaatg ttctgctgca
    17581 ccgcatattc acgcgcttca ctcagacgag catcgctcat taccgcacgc agctggttgc
    17641 tgccctggct gctaatcacc aggtccggat ggcgcttgct cagatacgca atggcttcat
    17701 cacgagcttt agcatcgcgg aaagtgatgc tcaggccgta gttgttttct ttacgaacag
    17761 tggtatacgg gatgcccttt tcgcgcaggt cactgcgtag gctatcgata ttttgttcct
    17821 ggagtttgcc aagcgcggtg tccatatcca cttccatcag gaagtgaacg ccgccacgca
    17881 ggtcaaggcc gagcttcatc ggctcagcat gaatagctgc cagccagcgc ggcgttgccg
    17941 gggcaaggtt aagcgccacg acgtatttgt cacccataac gcccattaat gcttcacgag
    18001 cgcgcaactg ggtgtcagtg gagtcgaagc gcgcaagaat agcgccctct tccagtgcca
    18061 cagacttagc agttattttt tcttcttgta acgttttctg gacctggatc agcgtttgct
    18121 cactggcggc gactccgcgc gcaccagtga tctgaacagc cggatcctca ccaaacaggt
    18181 tgggaagcgc atacagcaga ccgatgacaa tcaccacgat cagcatgacg tacttccaca
    18241 aaggataacg gtttaacacg gcaattccct tagggaaaaa ttttaattac agcgccttca
    18301 tggtgccttt cggcaggacg gcagctacga agtcacgttt aataactact tcagtggtgt
    18361 cattcagcgc gatagcaatg tagccgtttt ccgctacttt ggttacgcga ccaaccaggc
    18421 caccgttcgt cagaacttca tcacctttgg caatggagtc catcagcttt ttgtgttctt
    18481 tggtgcgctt ctgctgtgga cgcaggatca tgaaatagaa aatcagaccg aataccacca
    18541 gcatcaaaat caaagacatc gggctacctt gcgccggtgc acccgttgcc gctaccgcat
    18601 cagaaataaa aaagctcatt aaatttccct cattattaat attaatcaac gttcaaaggt
    18661 ggtacttctc gcccctgacg ctggtaaaaa tcagttacga agctctctaa tttaccctct
    18721 tcaatagcct tgcgtaaacc cgccatcaaa cgctggtagt aacgaaggtt atgaatggtg
    18781 ttgagtcgcg cgcctaatat ttcgttgcaa cggtcgagat gatgcaagta agcgcgtgaa
    18841 taattgcgac aggtgtagca atcacactca ggatcgagtg ggccagtatc gctcttatat
    18901 ttcgcattgc ggattttcac cacgccatcg gtcacgaaca aatgaccatt tcgggcgttg
    18961 cgggttggca ttacgcagtc aaacatatcg ataccgcgac gtacgccttc aaccaggtct
    19021 tccggtttac caacgcccat caggtaacgc ggtttgtctg ccggaatttg cggacacaca
    19081 tgctccagaa tacggtgcat atctgctttc ggctcaccca cagccagacc gccgacagcg
    19141 tagccatcaa agccgatatc taccagacct ttaacagata tatcacgtaa atcttcgtaa
    19201 atactgccct gaattatacc aaataacgca tttttgttac caagactgtc aaaacgctca
    19261 cggctacgct tcgcccaacg cagagacatc tccatagagc gttttgcgta atcccagtca
    19321 gcaggatacg gcgtacactc atcaaagatc atgacgatat ccgaaccaag atcgtactga
    19381 atctccatcg acttttccgg gtcgaggaaa atcgggtcgc cgttgatcgg gttacggaag
    19441 tgaacgccct gttcggtgat tttacgaata tcaccaaggc tgaagacctg gaagccgccg
    19501 gagtcggtaa gaatcggtcc tttccactgc ataaaatcgt gcagatcgcc gtgcagtttc
    19561 atgatttcct ggcccgggcg cagccacagg tggaaggtgt tgccgaggat aatttgcgcg
    19621 ccagtggctt caacttcttc cggcgtcatc ccttttacgg tgccgtaggt gccaacaggc
    19681 ataaaacaag gcgtttccac tacgccacga tcaaagacca ggcggccacg gcgtgcgcga
    19741 ccgtcggtgg tgtccagttc aaatttcatt ttttctccac tacgtcagaa aaacagtcca
    19801 acgtttaaac cagcgccgcg gaattactcc ccgacgcgct cattaattgc ctgcggattg
    19861 tacgtgataa acatcgcatc accgtaacta aaaaagcgat atttctcttc taccgccgct
    19921 ttataggcat tcatggtgtg ttgataaccg gcaaaggccg aaaccagcat aatcagcgtc
    19981 gactctggca agtggaagtt cgtcaccagc gcatcgacca ctttgtactg gaagcccgga
    20041 tagataaaga tttgggtatc gtcaaagaac ggttcgataa gatcgttttt tgccgcctgt
    20101 gccgcgcttt ccagcgaacg tactgaagtg gtgccaaccg caatcacccg gttaccgcgc
    20161 gctttcgccg ccagtaccgc gtctaccaca tcctgcggta cttcagcgta ttccgagtgc
    20221 atgatgtgat cttcaatggt gtcgacgcgc accggctgga aggtgcccgc accaacgtgc
    20281 aacgtcacaa acgccatctc cacgcctttg gcgcgtaatt tttccagcaa aggctcgtca
    20341 aaatgcagac cagcggtcgg ggctgcaacc gcgcccggtt tttcgctata aacggtttga
    20401 taaagttcgc ggtcagcgtc ttcgtccgga cgatcgatat acggcggcag cggcatatgg
    20461 ccgatgctgt tgaggatatc cagtaccgag cgttcatcat taaattcgac ttcaaacagt
    20521 gcgccgtggc gcgcggtcat tgttgcgttg atactttcgt catcgcccag caacagttct
    20581 gcgccaggtt ttggcgcttt cgaggcgcga atatgcgcaa gaatgcgttt gtcgtcgagc
    20641 atccgctcaa ccagcacttc aatcttgccg ccgctggctt tacgcccaaa cagacgcgcc
    20701 gggatcacgc gggtattatt aaaaaccaga agatcgccgg ggttgagctt atcgagtaaa
    20761 tcggtgaaag taccgtgcgt cagcgcgccc gtcggcccgt ccagcgacag taaacgacag
    20821 ctactgcgtt caggcatggg atagtgggca atcagggatt cgggcaattc aaaggagaaa
    20881 tcggtaacgc gcatgacact gactcagact aaaataagag tcgggtagtc tagtgccggg
    20941 gcgctctccc tgcaaccttt accccccttt ggatagaata taatcgatga attttttagc
    21001 tcacctgcat ttagcccatc tcgcggaaag ctcgctttct ggcaatttac tggctgattt
    21061 cgtacgcgga aatcctgaag aaagttttcc gcccgacgtc gtggctggca ttcatatgca
    21121 tcgacgtatt gactgacaat ctgccggaag tccgcgaagc acaggagtgg tttcgtagtg
    21181 aaacgcgccg cgttgcgcct attacgctgg atgtcatgtg ggatcacttt ctttcccgcc
    21241 actggtcgca gctgtcgccg gactttccgc tacaggaatt tgtctgttat gcccgcgagc
    21301 aagtgatgac gattttgccg gactcaccgc cacgttttat caatctgaac aattacttgt
    21361 ggtcagagca gtggctggtg cgctatcgcg atatggattt catccagaac gtgttaaacg
    21421 gcatggcaag ccgccgccca cgtctggatg ccctgcgtga ctcctggtac gatttagacg
    21481 ctcattatga cgccctcgaa acccgcttct ggcagtttta tccgcggatg atggcgcagg
    21541 cgtcacacaa ggcgttataa aacgttagtt catccatacc gtagccgaaa tggcaggcaa
    21601 agccagaatc ccgtcagtca gttgcccatg cccttctttg cattgccatt gcacggcatt
    21661 gagaaacggt gacgcgggta gcaccacttc actggcctcg ccacggttga ttgccaccag
    21721 tacacgttgc tgattcagca cgcggacaaa taccactacg ttatcttccg catacagcac
    21781 ctgacagccg ccacgacgca gcgcctgact tttcttacgc agcacaatca ttcgccggta
    21841 cagcgcgaat aacgccgtat cctgcttttc cacctgccag gggaacggtt tacggcaaaa
    21901 cggatcgttt ttaccatcca gtcccacttc atcaccgtaa taaatgcacg gtacaccagg
    21961 ccaggtgaac agccagacca ccgccagcgg caggcgcgca atatcccgac cgagcagcgt
    22021 tttaaatcgc gcagtatcgt ggctatcgag ctgattaaac atacgtaatt gttgttgatg
    22081 agaaagccct gcgcggtaat tatccatcca ggccatacag gtttgggcgt caatttgctg
    22141 cggatcataa gagatgtcgg tattggcaag aaatccccac aacgggaatg tgaagccacg
    22201 atagttcatg gcagcatctt ccacatcggc ctgtaaccat tgccgtgcat cgccaaaatg
    22261 ttcgccgaca atatacgctt ccggctgggt ttctttcgcc gcttcggtga tcccggcaac
    22321 gtgctgcata ttattgcgcg ccccacccgc ctcccccagc atatgcacca catccagccg
    22381 ccagccgtcc atattccacg gcgctttcag ccagtggcgg acaatactgt cttccccgcg
    22441 ataaatttca ttcaccagac tttccgactg ataatccagc ttcgtcaagc tggcatagcc
    22501 aagccagtcg agcgccgtgc catcatcact aaacgagtac cagtcgcgcc agggcgattc
    22561 agagttgtga caagctcccc ccgtgccaag attgtgcctg tcaaaccagg catgggaatc
    22621 gccactgtgg ttaaacacgc cgtccagcac cagccgcatt cccagctgct gcgtattgtg
    22681 tcgcaaacgc agcaacgccc catcaccgcc aaactgcgga tcgacatggc gataatcctc
    22741 ggtatcgtat ttatgtacgc tgggagcttt aaacaccgga ttgagataca gcgctgtcac
    22801 gccaagcttt ttcagatacg gcagtttttc gcttatcccg tccagatcgc cgccatagaa
    22861 cgttgatccg cccgcctgcg ccgtgaccgg ttcatcccag tcacgcaaga tgatctcttg
    22921 tccggctgca tgatggtaat agacatgatc ctgttcagct tcacgaggaa gactacgcgc
    22981 aaaacgatca gggaagatct gataaaaaat ctgatccgca gcccattgtg ggccgatatc
    23041 aggtacatcg acggcaaact gctccagtcg tgccggcggc attcggctga agccctgcgg
    23101 tgtaaaccaa cgctggcgat cgtgccacag cagtttgaaa ctgtaacgcc gccggggttg
    23161 tccgctggag agatcaatcg ccgcacgcca tgcggtgacg cctggctgcg gctgactgcg
    23221 ctgcttatgc attggtactg acatttcttc gttatcgtgt tctgtacgca gcataatgcg
    23281 ctgcggtggg tcttcgcccg tcagccacag tgtaatgagc agttgatctt tgctttgttt
    23341 aacaaatggg ggcaccggca ggtgccatgc atttaacatc ataattcccc tgtgatgaaa
    23401 atctgatcac cttgccatag cctaatgcag gatcactcat catctgaagg atatttgggg
    23461 gaggaggacg ggggcggata actttcggaa tagccggatc aggatttcct cctgaaccgg
    23521 cttacagaac gcaagtatta ttggttttca gccagctgac gatcgtggcg gcgtttaaac
    23581 atccagccaa tcaacagcac aacaatccac gcgaaaccga catacagcga gatacgcgta
    23641 tccgggtgat aaccaatcaa cccgataata aagagcagga aaatcagccc gccgatggtc
    23701 gttgctaccc cacccggcac tttaaatttc agcgccttaa cttcttctgg cggcaaacgg
    23761 cgacggaagg cgatttgcga cagcaggatc ataatccaca cccataccgt ggcgaaggtt
    23821 gccagcgaag cgatcaccag gaagacgttt tccggcatga tgtagttcag atacaccgca
    23881 aacagcagcg cggtagtcat caccagcacc gtaacccacg gaataccgcg acgcgacgtt
    23941 ttgctgaaaa ttttcggtgc gctgccctgc tctgccatac cgtggagcat acggcctacg
    24001 ccaaatacgt cactgttaat tgccgacagc gaagcggtca gcacaacaaa gttaagaatg
    24061 ctggcggcaa aggtaatgcc catatgctgg aacgtcagta cgaacgggct accggcagtg
    24121 ccaacctgat tccacgggta gatagacata atgacgaaca gcgtaccgac gtagaacacc
    24181 agaatacgca tcggcacgga gttaatcgca cgcggaatcg atttctcagg atctttcgct
    24241 tcaccggcgg taatcccgat aatttcgatc ccaccgtaag caaacatcac catttgcaac
    24301 gacattacca tgccaagcca gccgttactg aagaagccgc cgttgctcca caggttatga
    24361 ataccggtcg gttgcccgcc gttgccaatc ccccagatga tgatgccgaa accggcgaca
    24421 atcatgatga tgatggtggc gactttaaag aacgagaacc agaattccag ctcaccgaag
    24481 accttcacgc tcatcaggtt tacggcgcaa atgatcagca ccacgctcag tacccaaatc
    24541 cagtgcggca ccgtcgggaa caagacaccc atatagatac caaaagcggt cacatcggcg
    24601 atggcgacaa taaggatttc aaaacagtag gtccatccgg taatgtaacc tgccagcggg
    24661 ccaaggtttt cctgcgcata acgcgagaaa gagctggcgg ccgggttatg taccgacatt
    24721 tcccccagcg cacgcataat gatatacgcc gcgataccac cgataatata ggccaacaac
    24781 acgctcggac cggccatttt gatggcgtct gccgaaccgt aaaacagccc ggtgccaatt
    24841 gctgaaccca gtgccataaa gcgtatgtgg cgggtactta gcccacgctt tagcttattc
    24901 ttactttcca tcaattccta acccatcaac acaataaaaa accacggggc atcgcagccc
    24961 cgtggttaaa acaaatgttc agtgatttag tgagcgctgg aggtcacctg acgacctgcc
    25021 gcgcgatccc agataatggc cagaaccacc atcaccactg ttggcattaa ccacgccaga
    25081 ccttgttcgg ccagcggtaa acgctgcgcc caggacggta agatatcgct gaatgcagat
    25141 gctttgatcc cgtcgagaat accaaaaagc aggctgataa acatcggcgg agcaatcacg
    25201 cgggacgaat tatgccacca tgagcgtgta aaacttaata caaccagtgc gatacacggc
    25261 ggataaatag cggtcagcac cggtacggag atctggatca gctggcttaa gccgaggtta
    25321 gacaccacca tcgagaagcc gccgaggata aacaccagcg tacgataaga gagcggtacg
    25381 tactgggcaa agaattctgc acaagcacag gtcaggccaa ccgccgtgac cagacaggcg
    25441 atgaagatta acgccgccag caggaagcta ccgccgccgc caaaggtgtg ctgaacgtaa
    25501 gcatgcagaa tagcagcgcc gtttgcagac tgatcgacca gcgacgcgct gtctgaaccc
    25561 agacagaaca gcgccaggta cagcaaagtc agaccaacac ccgccatcag gccagcccag
    25621 acggtataac gggtcagcag acgcgcttcg gtaacgccac gagaacgcgc cgcgttaaca
    25681 ataacgatac caaacaccat tgcgcccagc gtatccatgg tcagatagcc gttaacgaag
    25741 ccgttagaaa atgcagcgtt ttgataagcc tcagtcgccg tgctgataga acccgccggc
    25801 cagacaatcg cgacaacaga caggatgacc agcgcgataa ttttcagcgg cgcaaggaag
    25861 ttgcccacgg tatccagcag cttgcccgga tagagcgaaa ccagaataac gatagcgaaa
    25921 tagaccaggc tgtagataaa cagcggcagc gcggaatcac ccgtcagcgg cgcaatcccc
    25981 acttcaaagg aaacggtagc tgtacgcggc gtagcgaaaa gcggccccac cgccaggtaa
    26041 caaaccgttg ccagcagtac gccagcgact ttaccgattg gcgtgctgag gctgtcaaca
    26101 ccgccgccaa cttttgccag cgccactacc gttaataccg gcaggccaac ggcagtaatg
    26161 aggaagccga atgccgcagt ccagacgtgt tcgcctgcct gtaaaccgac cattggaggg
    26221 aaaataatgt tacctgcgcc gacgaacaac gcaaatgtca taaagcccag agcgatgata
    26281 tcgcgcgatc ttaattgatg ggtcataaat catactgcct gtggatgtgg tgttgtaacg
    26341 ttaaattttt gtgccaatcc ctggggatga tacagcggta tatttaaaca attttagcgg
    26401 gaaatactcc caaccaggcg cggcgaagaa tagtttttcg atttaccgcg ttaatgcagt
    26461 cagtatgaca gcacctgaag cgcaatttaa acgcttataa cgttttaaag caaggaggga
    26521 tctaaaaacc agaggtatat agcgataaga aatcaccaac cagtataaaa cactatttcc
    26581 gtgaaaaaga catggctaat catgcgaaca aaaaatcacc aaccgttgta aaatctaacg
    26641 cccgtatttt gactgcaaag aaaataagcc agcatcggct ggcttatggc aataaaagat
    26701 gacaaaggcg gattaatcgc tgtttttggc aattaaacgt tccgggataa caaaactgaa
    26761 acgtgttcct tttcctactg tactctcaat attcaggcga ctttcgtgat gattcaccgc
    26821 atgtttcacg atcgctaacc ctaatccgct accgccggtt tgccgggaac gcgctttatc
    26881 aacgcgataa aaacgctcgg tcaggcgcgg aatatgctcc ggtgcaatgc ccggtccgtt
    26941 atcttcaacg ctaaattcgg caccgtgcgg cactcgctgc cagcgtacgg tgatatgcgt
    27001 gccttccggc gtatgattca cggcgttata gaccaggttc gaaatcgcac tgcgtagctg
    27061 atcttcgttg ccagacacct tgaggccgtt atctatctca aaggtaaatg tctgtttttt
    27121 ctgactcaga gtctgagcct cgcgctcaac aacgcgcagc atcatcggca catcaacctt
    27181 ttcattgagc aattgcgtcg gcgcggcttc aattttcgac agcgttagca actgcttcac
    27241 cagcccttcc atccgctggg tctgctcgcg catggtgtgc aacgcttttt cgcgtaccgc
    27301 gccttccagc ggctgctcat ccatcatctc caggtaaccc tgtaacacgg tcaatggcgt
    27361 acgtaactca tggctcacgt tggcaaaaaa gttacgccgc gccccttcca gttgatgcat
    27421 ttgcgtgaca tcacgcgcca ccatcagcaa ctgtttgtgg gtataaggca tgacgcgaat
    27481 ttccagatgc cgcccggtgt tgagcaccag attgagcggg cgagaaaaat cacgcgtttt
    27541 cagatattgc gtaaactccg ggtaacgcag taggttaagg atgttctgcc cgttatcttc
    27601 cggccagcgc aaaccaagaa tttgttgcgc cagaccgtta caccagaaaa taccgccctc
    27661 ttccgtggtc agcaccaccg cgtcgggcag cgactccgcg ccgctacgaa agcgtttaat
    27721 cagattgccc agttcacggc ggcgtttttt atttcgcagc tgcatctggt gtaagccgta
    27781 tagtagcggt tcccagctac cacgccccgg tggcggggtc atactgcgat ccacccacag
    27841 ccaccatgaa aggcgcaata aattccagaa atgccagata agcagtcctg ttaccgatgc
    27901 cagcaaaaac cagggcaggt aaccaaaaaa tgcacccagg atgaaagccg ggaggcagca
    27961 aagtagcagc tccagcacca gccttttcca cgacagccgt tccagcacgt aagatactcc
    28021 agttaagaaa tcataagccc tgctctgcgt ccgatgagca aggcgttaaa agcgggttga
    28081 aaaacgatat cctgtaccgc gcacggtctg caccatgcgg tcatgcccgc cgggctccag
    28141 tgctttacgc aggcgacgaa tgtggacatc gaccgtgcgg tcttccacat acacgttagt
    28201 tccccagacg tggtttaaca gctgctcgcg gctgtacacg cgctcaggat gcgtcataaa
    28261 aaagtgcagc agtttaaatt ctgtcggccc catctccagc ggctcttcgc ccgccatcac
    28321 tcggtgagat gtcgggttga gacttaatcc ctgcatctta atcacctctt ccaccgccat
    28381 tggcgaaata cggcgcatta ccgctttgat tcgcgccacc agctccttcg gcgaaaatgg
    28441 cttggtgata tagtcatccg cgccggtttc aaggccgcgc acgcgatctt cttcttcccc
    28501 tctggcggtc aacatcacca ctggaatatc ccgggtcatc gactcgcgct tgaggtgttt
    28561 gatgaactgg ataccggagc cgccaggtaa catccagtca aggagaatta aatccggcca
    28621 gggttcattc agttgattca cagcactgtc ataatcttcc gcttcgaccg gctgaaagcc
    28681 attttgttcg agcacgaagc agaccatttc gcgaattgga gcttcatctt ctacgaccag
    28741 aatacgtctc gccatgattt gccctgttgt aataaatagg ttgcgatcat taatgcgacg
    28801 tcattatgcg tcagatttat gacagattta tgaaaagctc gtcgcacata tcttcaggtt
    28861 attgatttcc gtggcgcaga aaaaagcaaa tggcacatct gtttgggtat aatcgcgacc
    28921 atgctttttc gccagggaac cgttatgcgc atccttcaca cctcagactg gcatctcggc
    28981 cagaacttct acagtaaaag ccgcgaagct gaacatcagg cttttcttga ctggctgctg
    29041 gagacagcac aaacccatca ggtggatgcg attattgttg ctggtgatgt tttcgatacc
    29101 ggctcgccgc ccagttacgc ccgcacgtta tacaaccgtt ttgttgtcaa tttacagcaa
    29161 actggctgtc atctggtggt actggcagga aaccatgact cggtcgccac gctgaatgaa
    29221 tcgcgcgata tcatggcgtt cctcaataca accgtggtcg ccagcgccgg acatgcgccg
    29281 caaatcttgc ctcgtcgcga cgggacgcca ggcgcagtgc tgtgccccat tccgttttta
    29341 cgcccgcgtg acattatttc cagccaggcg gggcttaacg gtattgaaaa acagcagcat
    29401 ttactggcag cgattaccga ttattaccaa caacactatg ccgatgcctg caaactgcgc
    29461 ggcgatcagc ctctgcccat catcgccacg ggacatttaa cgaccgtggg ttccagtaaa
    29521 agtgacgccg tgcgtgacat ttatattggc acgctggacg cgtttccggc acaaaacttt
    29581 ccaccagccg actacatcgc gctcgggcat attcaccgcg cacagattat tggcggcatg
    29641 gaacatgttc gctattgcgg ttcccccatt ccactgagtt ttgatgaatg cggtaagagt
    29701 aaatatgtcc atctggtgac attttcaaac ggcaaattag agagcgtgga aaacctgaac
    29761 gtaccggtaa ctcaacccat ggcggtgctg aaaggcgatc tggcgtcgat taccgcacag
    29821 ctggaacagt ggcgcgatgt atcgcaggag ccacctgtct ggctggatat cgaaatcact
    29881 actgatgagt atctgcatga tattcagcgc aaaatccagg cattaaccga atcattgcct
    29941 gtcgaagtat tgctggtacg tcggagtcgt gaacagcgcg agcgtgtgtt agccagccaa
    30001 cagcgtgaaa ccctcagcga actcagcgtc gaagaggtgt tcaatcgccg tctggcactg
    30061 gaagacctgg atgaatcgca gcagcaacgt ctgcagcatc ttttcaccac gacgttgcgt
    30121 accctcgccg gagaacacga agcatgaaaa ttctcagcct gcgcctgaaa aacctgaact
    30181 cattaaaagg cgaatggaag attgatttca cccgcgagcc gttcgccagc aacgggctgt
    30241 ttgccattac cggcccaacc ggtgcgggga aaaccaccct gctggacgcc atttgtctgg
    30301 cgctgtatca cgaaactccg cgtctctcta acgtttcaca atcgcaaaat gatctcatga
    30361 cccgcgatac cgctgaatgt ctggcggagg tggagtttga agtgaaaggt gaagcgtatc
    30421 gcgcgttctg gagccagaat cgggcgcgta accagccgga cggtaatttg caggtgcctc
    30481 gcgtagagct ggcgcgctgc gccgacggca aaattctcgc cgacaaagtg aaagataagc
    30541 tggaactgac agcgacgtta accgggctgg attacgggcg cttcacccgt tcgatgctgc
    30601 tttcgcaggg gcaatttgct gccttcctga atgccaaacc caaagaacgc gcggaattgc
    30661 tcgaggagtt aaccggcact gaaatctacg ggcaaatctc ggcgatggtt tttgagcagc
    30721 acaaatcggc ccgcactgag ctggagaagc tgcaagcgca ggccagcggc gtcacgttgc
    30781 tcacgccgga acaagtgcaa tcgctgacag cgagtttgca ggtacttact gacgaagaaa
    30841 aacagttaat taccgcgcag cagcaagaac aacaatcgct aaactggtta acgcgtcagg
    30901 acgaattgca gcaagaagcc agccgccgtc aacaggcctt gcaacaggcg ttagccgaag
    30961 aagaaaaagc gcaacctcaa ctggcggcgc ttagtctggc acaaccggca cgaaatcttc
    31021 gtccacactg ggaacgcatc gcagaacaca gcgcggcgct ggcgcatatt cgccagcaga
    31081 ttgaagaagt aaatactcgc ttacagagca caatggcgct tcgcgcgagc attcgccacc
    31141 acgcggcgaa gcagtcagca gaattacagc agcagcaaca aagcctgaat acctggttac
    31201 aggaacacga ccgcttccgt cagtggaaca acgaactggc gggttggcgt gcgcagttct
    31261 cccaacaaac cagcgatcgc gagcatctgc ggcaatggca gcaacagtta acccatgctg
    31321 agcaaaaact taatgcgctt gcggcgatca cgttgacgtt aaccgccgat gaagttgcta
    31381 ccgccctggc gcaacatgcc gagcaacgcc cactgcgtca gcgcctggtc gcgctgcatg
    31441 ggcagattgt tccccaacaa aaacgtctgg cgcagttaat ggtcactatc cagaatgtca
    31501 ctctggaaca gacgcaacgt aatgtcgcac ttaacgaaat gcgccagcgt tataaagaaa
    31561 agacgcagca acttgccgat gtgaaaacca tttgcgagca ggaagcgcgc atcaaaacgc
    31621 tggaagccca gcgtgcacag ttacaggcgg gtcagccttg tccactttgt ggttccacca
    31681 gccacccggc ggtcgaggcg tatcaggcgc tggagcctgg tgttaatcag tctcgattac
    31741 tggcgctgga aaacgaagtt aaaaagctcg gcgaagaagg tgcggcgctg cgcgggcaac
    31801 tggatgcatt aacgaagcag cttcagcgcg atgaaaacga agcgcaaagc ctccgacaag
    31861 atgagcaagc acttactcaa caatggcaag ccgtcacggc cagcctcaat atcaccttgc
    31921 agccacagga cgatattcaa ccgtggctgg atgcacaaga tgagcacgaa cgccagctgc
    31981 ggttactcag ccaacgacat gaattacaag ggcagattgc cgcgcataat cagcaaatta
    32041 tccagtatca acagcaaatt gaacaacgtc agcagcagct cttaacggca ttggcgggtt
    32101 atgcactgac attgccacag gaagatgaag aagagagctg gttggcgaca cgtcagcaag
    32161 aagcgcagag ctggcagcaa cgccagaacg aattaaccgc gctgcaaaac cgtattcagc
    32221 agctgacacc gattctggaa acgttgccgc aaagtgatga tctcccgcac agcgaagaaa
    32281 ctgtggcgct ggataactgg cggcaggttc atgaacaatg tctcgcatta cacagccagc
    32341 agcagacgtt acagcaacag gatgttctgg cggcgcaaag tctgcaaaaa gcccaagcgc
    32401 agtttgacac cgcgctacag gccagcgtct ttgacgatca gcaggcgttc cttgcggcgc
    32461 taatggatga acaaacacta acgcagctgg aacagctcaa gcagaatctg gaaaaccagc
    32521 gccgtcaggc gcaaacgctg gtcactcaga cagcagaaac gctggcacag catcaacaac
    32581 accgacctga cgggttggct ctcactgtga cggtggagca gattcagcaa gagttagcgc
    32641 aaactcacca aaagttgcgt gaaaacacca cgagtcaagg cgagattcgc cagcagctga
    32701 agcaggatgc agataaccgt cagcaacaac aaaccttaat gcagcaaatt gctcaaatga
    32761 cgcagcaggt tgaggactgg ggatatctga attcgctaat aggttccaaa gagggcgata
    32821 aattccgcaa gtttgcccag gggctgacgc tggataattt agtccatctc gctaatcagc
    32881 aacttacccg gctgcacgtg cgctatctgt tacagcgcaa agccagcgag gcgctggaag
    32941 tcgaggttgt tgatacctgg caggcagatg cggtacgcga tacccgtacc ctttccggcg
    33001 gcgaaagttt cctcgttagt ctggcgctgg cgctggcgct ttcggatctg gtcagccata
    33061 aaacacgtat tgactcgctg ttccttgatg aaggttttgg cacgctggat agcgaaacgc
    33121 tggataccgc ccttgatgcg ctggatgccc tgaacgccag tggcaaaacc atcggtgtga
    33181 ttagccacgt agaagcgatg aaagagcgta ttccggtgca gatcaaagtg aaaaagatca
    33241 acggcctggg ctacagcaaa ctggaaagta cgtttgcagt gaaataacta ttcagcagga
    33301 taatgaatac agaggggtga attatctctt ggccttgctg gtcgttatcc tgcaagctat
    33361 cactttattg gctacggtga ttggtagccg ttctggtggt tgtgatggtg gtatgaaaaa
    33421 agtcatttta tctttggctc tgggcacgtt tggtttgggg atggccgaat ttggcattat
    33481 gagcgtgctc acggagctgg cgcataacgt aggaatttcg attcctgctg ccgggcatat
    33541 gatctcgtat tatgcactgg tggtggtggt cggtgcgcca atcatcgcac tcttttccag
    33601 ccgctactca ctcaaacata tcttgttgtt tctggtggcg ttgtgcgtca ttggcaacgc
    33661 catgttcacg ctctcttcgt cttacctgat gctcgccatt ggtcggctgg tatccggctt
    33721 tccgcatggc gcattttttg gcgtcggagc gatcgtgtta tcaaaaatta tcaaacccgg
    33781 aaaagtcacc gccgccgtgg cggggatggt ttccgggatg acagtcgcca atttgctggg
    33841 cattccgctg ggaacgtatt taagtcagga atgctggcgt tacacctttt tattgatcgc
    33901 tgtttttaat attgcggtga tggcatcggt ctatttttgg gtgccagata ttcgcgacga
    33961 ggcgaaagga aatctgcgcg aacaatttca ctttttgcgc agcccggccc cgtggttaat
    34021 tttcgccgcc acgatgtttg gcaacgcagg tgtgtttgcc tggttcagct acgtaaagcc
    34081 atacatgatg tttatttccg gtttttcgga aacggcgatg acctttatta tgatgttagt
    34141 tgggctaggg atggtgctgg gaaatatgct aagtggcagg atttcaggac gttattcacc
    34201 actgcgcatt gcagcagtga ctgactttat cattgtactg gcactgctga tgctcttttt
    34261 ctgcggcggc atgaaaacaa cgtcgcttat ttttgctttt atttgttgcg cgggattatt
    34321 tgccctttca gcacctctgc aaatattgtt actgcaaaat gccaaaggag gagagttatt
    34381 aggtgccgca ggtgggcaaa tagcgtttaa cctcggtagc gccgtcggcg catattgcgg
    34441 aggtatgatg ctgacgctgg ggctggcata taattacgtg gcgctgcctg ccgccctgct
    34501 ttcgtttgct gcgatgtcat cgttgctgct gtatggtcgc tataagcgcc agcaagcggc
    34561 ggatagtccg gtgctggcga aaccactggg gtagattact gtcacggtgc gcgttgctta
    34621 ttgccggatg cggcgtgaac gccttatccg ccctacgcgg ttctggcaca ttttgtaggc
    34681 ctgataagac gcggcaagcg tcgcatcagg catgatgcgc caattgccta cgttttttac
    34741 tcttgtggcc ataaccacgc agcgccgcgt acgccgctgg aatcaccgtg cttcgcctta
    34801 ctcaccggcg tttcacattc gccgccgaag acaaattgtt taatcaactg cccaaccgtt
    34861 tgatataaac ggtctacatt gctcatcccg ccccccagga caatcacatc cggatcgaga
    34921 atattcacga catgtgccag cgattttgcc agccgcagct cgtagcgacg caatgccagt
    34981 tccgctaccg gatcgctttc ttcaaccagg cggataattt cactgccttt cagcgcatgt
    35041 ccgctcaaac gacgataatc cgtcgcgaat cccgtgcccg aaataaaggt ttcaatacaa
    35101 ccttgtttac cgcaataaca agggacttcc tcgcgataac gcagttcgtc ttcgtccatc
    35161 cacggtagcg gattgtgtcc ccactcacct gccgtgccat tgccgccgat atgcgtccgc
    35221 ccattgaatg ccacgcccgc gccgcatccc gtgccgataa tcacggcaaa taccgtctgc
    35281 gctcccgctg ccgcgccatc tactgcttct gaaaccgcca gacagttggc atcatttgcc
    35341 agccgcactt cccgctgcaa cctcgcgctt aagtctttat cgaatggctg accgttgagc
    35401 caggttgaat tgacattctt caccacaccg gtgtagggcg aaatcgaacc tggaatgccc
    35461 atacctaccg ttccgcgctg ccctgtcgcc tgctccgcca tatcaaccaa cgtggcgatc
    35521 gtttcaatag tctgccggta atcatcacgc ggcgtgggca gacgatggcg gtacaactgc
    35581 tcccctgcat cgcccagtgc aatcacttca gttttggtgc cgcctaaatc gatacctata
    35641 cgcactgtac tctccttatt tttttcaata tcaatagcgt agagacggac aaccggattg
    35701 gcaatgcaag gccgccgaca attcgttatc atgcccgcta aatttaacga caaggccgtg
    35761 gaaattatca tgctgtggtt caaaaattta atggtttacc gtcttagccg cgagatttcg
    35821 ctgcgtgcag aagagatgga aaaacagcta gcctcgatgg catttacccc atgcggcagc
    35881 caggacatgg cgaagatggg ctgggttcct ccgatgggat cgcacagcga tgcgttaacg
    35941 cacgttgcca atggtcaaat tgttatctgc gcgcgcaaag aagaaaaaat cctcccgtct
    36001 ccggtgatta aacaggcgct ggaagcgaaa atcgccaaac tggaagcgga acaggcgcgt
    36061 aagctgaaga aaaccgaaaa agattcgctg aaagacgaag tgctgcactc tctgctgccg
    36121 cgtgctttca gccgttttag ccagacaatg atgtggctcg acacggttaa cggtttgatt
    36181 atggtggact gcgccagtgc caaaaaagcg gaagatacgc tggcattact gcgtaaaagc
    36241 ctggggtcgt taccggttgt accattgagc atggaaaacc cgattgaact gacgctgacc
    36301 gaatgggttc gctccggtag tgcggcacag ggcttccagc tgcttgatga agccgagctg
    36361 aaatcgttgc tggaagatgg cggcgtgatc cgcgcgaaga aacaagatct gaccagcgaa
    36421 gagatcacca atcacattga agccggaaaa gtggtgacta aactggcgct cgactggcag
    36481 cagcgcattc agtttgtgat gtgcgacgat ggttcgctca agcgtcttaa gttctgcgac
    36541 gagctgcgcg atcaaaacga agatatcgac cgtgaagatt tcgcccagcg ttttgatgcc
    36601 gatttcatcc tgatgactgg tgaactggca gcgttaattc aaaacctgat tgaaggatta
    36661 ggtggcgaag cacaacgtta attgctgatt ttcctttaat gccggatgcg acgcctgccg
    36721 cgtcttatcc ggcgtacgaa gccacaccag gcatataatt attcgctacg gcgagcaata
    36781 atttttagcg cagcaatatt atgcgtttta cgccgtaact gatctaatcg cctcatccct
    36841 attcgcctgt cagcagcacg cagaatattc actaaccccc atttatcgct ggtaaattgt
    36901 tttgttaaca gctccgcttt tggggtatca atatattcac gcaataaatc agaaattgaa
    36961 caaatatccg tcaggtaagg ataccagcaa gtttcaccgt gaaaattacg cactccagcg
    37021 ttacctgcaa taaagtcttt cgggtaatcc cagatcacgt ttttatccaa cgtgatccaa
    37081 taacgcggta aatcggtact tccaccattt tggctgcgca tgggataacg ggtacagtga
    37141 atttgcaggt tgatcgttgg cgtcagtaag tcatagatct cccgctgtaa cttgctccat
    37201 ggacgttgtg tcattgtttt tcctcaagcc ggtgacgcgc ttcactgcga attttccgcg
    37261 ttgccccggt tttcactaaa tcacgtaact gcgaaagatc gggttgttcg acaaataaga
    37321 gtaatgatgg atcctctttt aaccacgccg ttttcacatc ggcagccagc tgtggattat
    37381 taatagccag cgaccgatcc tgaggctcag tcaatgtcgt taacaacgac gccggggtat
    37441 gtgcgttgcg taatgccgtt tggcgcaaat gatcattgcg actttgcagc aatgcacgcc
    37501 accattcatc atctgattta tcttttatcg tagccagttc cgcaaaaaat acctggagat
    37561 cttcactgat atttatatca accttgatga attatgaata tatcggaaat tgaaaaaatc
    37621 gcagcaaagg aaatattatt atgggttaaa gagaggatat ttcagaaaaa tgccctcccg
    37681 ttcaagggga aggcgaggaa ttacagatag cggcacagat aagaggtagg ttcggcaact
    37741 tgaagatgaa actcactgtg accgggaaca ttaaacaccg aaccggcttc atacacttgc
    37801 cagtcggtcg cgtcaggcag taacacattc agcgcgccac tgattaccgt catctcttcc
    37861 ggctcagcgg tgctgaaggt gtattcgcct tcaaccataa cacccacgct ggcgcgacca
    37921 gtgctgctgc tggaaaagcc gatcgatttc actttgccgg aaaagtactc attactttga
    37981 agcataaact ggccctgtct ggtagttgaa catctacttc actatagggg ccagaggcgc
    38041 tggctgtcac gcaaaattac accagtaatt ccgcagccag ccgtgcaatc aatacgttag
    38101 agagcaagac aggaacatcg agctgttttt gcagtaaatc gcgatgacgt tggtgaaatc
    38161 ctaaacaatc cagcatgatg acatcagccc cttttgccag taattctttc ccagcatcaa
    38221 tgattttttg ttctgaatca tgaatggggt tacccaatga aaataccggc gatttctgca
    38281 aaatttgcca tttttgcgcc tgaacgggca gcatctcctc aaccggaacg ataaccccca
    38341 cctgatgatc ttcaacaata gaggaaacca gtggaggcaa tattcgcgac ggctcaagaa
    38401 agatcgtatt acgcgcagtc atactactaa tgtttgctgt actcattaat aaaatgacgt
    38461 cataaccctg attatcgagc acttcaacca caccttgcag atctcgctcc actttacgac
    38521 gcgaaacatg ggccagctgg ttgtcattta ataatgtgag aatggtgtct tcgcctgctt
    38581 ctggcgcgta ctccgccatc acttcttcac gacttaactt ccccagcagg ctatgatggg
    38641 aaatattatc ttcgtcaatg tattccgtca ggagcggcaa aacctcctgc ataggtacaa
    38701 tgccgatggt caggatcgcc aacgacgcac tcattgttca tccaccttac ttttctttcg
    38761 tcgttaatta ccgggcaagt gtgaagcacc actgctgaca cttacttgtg ctacaaatga
    38821 caaaaagcgt agcagcagac gtgcacggca taatgtaaag attccaaatg attccagtaa
    38881 tggaatgtta ttgtttaata ttctaattat tagaaaaaca taaattatga aaaatgtgac
    38941 gcagatccct tgtagtggca ctcagaatcc cttccgagca gtctgatgcg gtcactcttt
    39001 gtcagggtcc tcatagcgtt ttttcgcatc catcgcttcc tgagccgtcg gatgttcact
    39061 gatcaacgag tctggtttag gatgatcggc tctgagttgg taccaggtta ccgtctgtcc
    39121 tggctttcct ttttcaatcg tcactatata tgcttcacga ggataaggcg gtttcgttgg
    39181 catcgttctt ccttatttca cgggatgaac gttaagtata ggcgctctaa aatcaacaat
    39241 tgatcgtctg tgccagggcg ctacgaattt cagaaatcac ctggctgggt tcgtttgttg
    39301 cgtcgatgat aatatgcgca acttcgcgat atagcgcatc gcgttcttcc agcacttcct
    39361 gaacttcttc gctcagcggt tttcccgtta aggttggccg taaatcttct tccggtgcag
    39421 cttgcagtcg gttaaccagg actgatactg gcgcacacaa ataaaccacg atcccgttat
    39481 tttgcatgaa gtgacgatta aattccgtca gaataatgcc gccgcctgta gcgataacgg
    39541 tggatgccgc agttaccgct tccagcgccg ccgtttctct ggcgcgaaat cccgcccact
    39601 cttccctttc gacgatctcc gcgaccgtca tattgagctg tgattgcaac cactgatcgg
    39661 tatcgacaaa ccgacggtta agcgaatcgg caagggccat tccgaccgtt gttttaccac
    39721 agccccgagg cccgatcaga aaaagaggtt gtgtcatcgt gggttttccc caataggtcg
    39781 caatgcggcg aaagccggtg tcatgagaat agcgatcata ccatcaaact agtacaattt
    39841 cgattgtaaa gaaaaaattc cacttaaagt aaaaatctca atacatccct tactatacca
    39901 ataaatattc aagaatgaag tgtaaataat aaattacatt tagccacgac tacgttgcac
    39961 ttccagccac cacttctcca gctccgcggc aaaggcctgg cggtcacgtt gtgaaaggct
    40021 atctggtccg ccggtctgga tcccactggc acgtaaggta tccataaaat cgcgcatcgt
    40081 caggcactca cgaatggtcg ctggcgtgta acgttcgccg cgcgaattaa gcgccgctgc
    40141 gcctttctcg atggcttcag cagccaaagg tatatctgcg gtgatcacca aatcgcccgc
    40201 ttcacactgc cggacaattt cgttatcggc aacgtcgaaa cctgccgcga cgcgcagcgt
    40261 acgaataaat cgcgatggcg gcacgcgtaa actctggttt gctaccagta ccagcggcat
    40321 ctgcatacgt tccgccgcgc gatacaaaat ctctttaatt acattgggac acgcgtcggc
    40381 atccacccaa attgtcataa agtcatcctt tgttaggtaa tcctctattg tgtcgcgctt
    40441 ttgccttccg gcatagttct gtttatgctt ctgccagcga ttatcaaaac aatgaatttc
    40501 acggcaggag tgaggcaatg gaaaagaaaa tcggttttat tggctgcggc aatatgggaa
    40561 aagccattct cggcggtctg attgccagcg gtcaggtgct tccagggcaa atctgggtat
    40621 acaccccctc cccggataaa gtcgccgccc tgcatgacca gttcggcatc aacgccgcag
    40681 aatcggcgca agaagtggcg caaatcgccg acatcatttt tgctgccgtt aaacctggca
    40741 tcatgattaa agtgcttagc gaaatcacct ccagcctgaa taaagactct ctggtcgttt
    40801 ctattgctgc aggtgtcacg ctcgaccagc ttgcccgcgc gctgggccat gaccggaaaa
    40861 ttatccgcgc catgccgaac actcccgcgc tggttaatgc cgggatgacc tccgtaacgc
    40921 caaacgcgct ggtaacccca gaagataccg ctgatgtgct gaatattttc cgctgctttg
    40981 gcgaagcgga agtaattgct gagccgatga tccacccggt ggtcggtgtg agcggttctt
    41041 cgccagccta cgtatttatg tttatcgaag cgatggccga cgccgccgtg ctgggcggga
    41101 tgccacgcgc ccaggcgtat aaatttgccg ctcaggcggt aatgggttcc gcaaaaatgg
    41161 tgctggaaac gggagaacat ccgggggcac tgaaagatat ggtctgctca ccgggaggca
    41221 ccaccattga agcggtacgc gtactggaag agaaaggctt ccgtgccgca gtgatcgaag
    41281 cgatgacgaa gtgtatggaa aaatcagaaa aactcagcaa atcctgatga ctttcgccgg
    41341 acgtcaggcc gccacttcgg tgcggttacg tccggctttc tttgctttgt aaagcgccaa
    41401 atctgccgat ttcaaccact cacgatagtg actcatttgt gggttcagcg gcgcaacccc
    41461 cacactaatc cgtaaagtta cctgtggcgt attcggcaag cgtaacgtgt ttagcccttc
    41521 acgcacccgt aacatggcgg taatggcgct ctcagctggc gtaccggaca tgattactgc
    41581 aaactcatcg ccgccaaacc gaccaatcac atcgctaccg cgcagggtaa tttgtaactg
    41641 tcgggtaagc gccacaatcg cttcatcgcc cacatcatga ccccaggtat cgttgatgct
    41701 cttgaaatgg tcgatatcga taatcagtaa cgttgcatcg cgattatgcc gccgacagtt
    41761 atcaaattca ttgcgtaaca tagtttccca atgacgtcgg ttatacacgc ccgtcatgcc
    41821 gtcgcgagta ctcatgacct gcaacctgcg tttatgttcc gccagtttgg ttgccgtctg
    41881 gtagctgacc cagccaaaca tcagaggata aacgacaata atgggaaggg agagccacca
    41941 ttccagcgga gcactattga acgacacggt aatgcccgta agctcgaggg tgacaaggca
    42001 ggaaaccacc atcaacacca gacccgcgac aaacagacgg gggccgcctg cccccatcaa
    42061 attcagacac agaatcatca acatagcggt ggaaggcagc acgtttacgc ccattacgcc
    42121 tacccacatt cccgctaata ctgcatcggt ttttaagttg taaatttccc ggctaagcgg
    42181 atcgacggcc ctgctcgcta tctgccaggc taaatgcggc cagacgaacg cccagccgac
    42241 caacaccagc caccaccagc ccggcggcgg gtgcgaaacc agcgttgaag caatcggtaa
    42301 gaacatgcca gccaggccaa ccgcacgggg tagtctgacg cgacgggcga agcgcagccc
    42361 ggaccgctga tgttcgtttt gaggagttaa aggaggttcc tccccatgcg ccgccgcttt
    42421 tttgaaaaag ttttcatcat tcattatttt tgggaacatt cagaaacaat tttcccaaat
    42481 tatagagacg gggctaaagg cagaagtatg aaatttcggg tgagccactt tcgtagctca
    42541 cccgttagca ct
//
LOCUS       DAZSNO010000019        40956 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZSNO010000019 DAZSNO010000000
VERSION     DAZSNO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40956
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.019"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(87..1337)
                     /gene="icd"
                     /locus_tag="ACHWN2_000978"
     CDS             complement(87..1337)
                     /gene="icd"
                     /locus_tag="ACHWN2_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR8930614.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1509..2162
                     /gene="rluE"
                     /locus_tag="ACHWN2_000979"
     CDS             1509..2162
                     /gene="rluE"
                     /locus_tag="ACHWN2_000979"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR8930615.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2172..2633
                     /gene="nudJ"
                     /locus_tag="ACHWN2_000980"
     CDS             2172..2633
                     /gene="nudJ"
                     /locus_tag="ACHWN2_000980"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR8930616.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2687..3793
                     /gene="mnmA"
                     /locus_tag="ACHWN2_000981"
                     /gene_synonym="trmU"
     CDS             2687..3793
                     /gene="mnmA"
                     /locus_tag="ACHWN2_000981"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR8930617.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3829..4470
                     /gene="hflD"
                     /locus_tag="ACHWN2_000982"
     CDS             3829..4470
                     /gene="hflD"
                     /locus_tag="ACHWN2_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR8930618.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4474..5844
                     /gene="purB"
                     /locus_tag="ACHWN2_000983"
     CDS             4474..5844
                     /gene="purB"
                     /locus_tag="ACHWN2_000983"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR8930619.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6013..6684
                     /gene="phoP"
                     /locus_tag="ACHWN2_000984"
     CDS             6013..6684
                     /gene="phoP"
                     /locus_tag="ACHWN2_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR8930620.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6684..8144
                     /gene="phoQ"
                     /locus_tag="ACHWN2_000985"
     CDS             6684..8144
                     /gene="phoQ"
                     /locus_tag="ACHWN2_000985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR8930621.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8941..9222)
                     /locus_tag="ACHWN2_000986"
     CDS             complement(8941..9222)
                     /locus_tag="ACHWN2_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930622.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9236..10897)
                     /locus_tag="ACHWN2_000987"
     CDS             complement(9236..10897)
                     /locus_tag="ACHWN2_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR8930623.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10881..11207)
                     /locus_tag="ACHWN2_000988"
     CDS             complement(10881..11207)
                     /locus_tag="ACHWN2_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR8930624.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11263..11445)
                     /locus_tag="ACHWN2_000989"
     CDS             complement(11263..11445)
                     /locus_tag="ACHWN2_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930625.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11429..11869)
                     /locus_tag="ACHWN2_000990"
     CDS             complement(11429..11869)
                     /locus_tag="ACHWN2_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR8930626.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11869..12165)
                     /locus_tag="ACHWN2_000991"
     CDS             complement(11869..12165)
                     /locus_tag="ACHWN2_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFR8930627.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12162..12500)
                     /locus_tag="ACHWN2_000992"
     CDS             complement(12162..12500)
                     /locus_tag="ACHWN2_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFR8930628.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12497..13708)
                     /locus_tag="ACHWN2_000993"
     CDS             complement(12497..13708)
                     /locus_tag="ACHWN2_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR8930629.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13710..14282)
                     /locus_tag="ACHWN2_000994"
     CDS             complement(13710..14282)
                     /locus_tag="ACHWN2_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFR8930630.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14322..15479)
                     /locus_tag="ACHWN2_000995"
                     /pseudo
     CDS             complement(14322..15479)
                     /locus_tag="ACHWN2_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15780..16004)
                     /locus_tag="ACHWN2_000996"
     CDS             complement(15780..16004)
                     /locus_tag="ACHWN2_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930631.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16045..16158)
                     /locus_tag="ACHWN2_000997"
     CDS             complement(16045..16158)
                     /locus_tag="ACHWN2_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR8930632.1"
                     /translation="MSRVGISEPHHAAGAIRLFLRHLKRYGALLAFRHGPA"
     gene            complement(16233..16307)
                     /locus_tag="ACHWN2_000998"
                     /pseudo
     CDS             complement(16233..16307)
                     /locus_tag="ACHWN2_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16317..16622)
                     /locus_tag="ACHWN2_000999"
     CDS             complement(16317..16622)
                     /locus_tag="ACHWN2_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930633.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16619..16897)
                     /locus_tag="ACHWN2_001000"
     CDS             complement(16619..16897)
                     /locus_tag="ACHWN2_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930634.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17186..18934)
                     /locus_tag="ACHWN2_001001"
     CDS             complement(17186..18934)
                     /locus_tag="ACHWN2_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFR8930635.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18931..19230)
                     /locus_tag="ACHWN2_001002"
     CDS             complement(18931..19230)
                     /locus_tag="ACHWN2_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR8930636.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19236..19469)
                     /locus_tag="ACHWN2_001003"
     CDS             complement(19236..19469)
                     /locus_tag="ACHWN2_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930637.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19471..19691)
                     /locus_tag="ACHWN2_001004"
                     /pseudo
     CDS             complement(19471..19691)
                     /locus_tag="ACHWN2_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19684..19905)
                     /locus_tag="ACHWN2_001005"
     CDS             complement(19684..19905)
                     /locus_tag="ACHWN2_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930638.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19895..20095)
                     /locus_tag="ACHWN2_001006"
     CDS             complement(19895..20095)
                     /locus_tag="ACHWN2_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR8930639.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20225..20404
                     /locus_tag="ACHWN2_001007"
     CDS             20225..20404
                     /locus_tag="ACHWN2_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930640.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20529..20858
                     /locus_tag="ACHWN2_001008"
     CDS             20529..20858
                     /locus_tag="ACHWN2_001008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930641.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20908..21096)
                     /locus_tag="ACHWN2_001009"
     CDS             complement(20908..21096)
                     /locus_tag="ACHWN2_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8930642.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21471..22700)
                     /locus_tag="ACHWN2_001010"
     CDS             complement(21471..22700)
                     /locus_tag="ACHWN2_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8930643.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22949..24070
                     /gene="roxA"
                     /locus_tag="ACHWN2_001011"
                     /gene_synonym="ycfD"
     CDS             22949..24070
                     /gene="roxA"
                     /locus_tag="ACHWN2_001011"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR8930644.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24119..25345)
                     /gene="pepT"
                     /locus_tag="ACHWN2_001012"
     CDS             complement(24119..25345)
                     /gene="pepT"
                     /locus_tag="ACHWN2_001012"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR8930645.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25595..26731
                     /gene="potA"
                     /locus_tag="ACHWN2_001013"
     CDS             25595..26731
                     /gene="potA"
                     /locus_tag="ACHWN2_001013"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR8930646.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26715..27572
                     /gene="potB"
                     /locus_tag="ACHWN2_001014"
     CDS             26715..27572
                     /gene="potB"
                     /locus_tag="ACHWN2_001014"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR8930647.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27569..28363
                     /gene="potC"
                     /locus_tag="ACHWN2_001015"
     CDS             27569..28363
                     /gene="potC"
                     /locus_tag="ACHWN2_001015"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR8930648.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28360..29406
                     /gene="potD"
                     /locus_tag="ACHWN2_001016"
     CDS             28360..29406
                     /gene="potD"
                     /locus_tag="ACHWN2_001016"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR8930649.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29562..30383)
                     /gene="cobB"
                     /locus_tag="ACHWN2_001017"
     CDS             complement(29562..30383)
                     /gene="cobB"
                     /locus_tag="ACHWN2_001017"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR8930650.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30399..31310)
                     /gene="nagK"
                     /locus_tag="ACHWN2_001018"
     CDS             complement(30399..31310)
                     /gene="nagK"
                     /locus_tag="ACHWN2_001018"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR8930651.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31339..32583)
                     /gene="lolE"
                     /locus_tag="ACHWN2_001019"
     CDS             complement(31339..32583)
                     /gene="lolE"
                     /locus_tag="ACHWN2_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR8930652.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32583..33284)
                     /gene="lolD"
                     /locus_tag="ACHWN2_001020"
     CDS             complement(32583..33284)
                     /gene="lolD"
                     /locus_tag="ACHWN2_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR8930653.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33277..34476)
                     /gene="lolC"
                     /locus_tag="ACHWN2_001021"
     CDS             complement(33277..34476)
                     /gene="lolC"
                     /locus_tag="ACHWN2_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR8930654.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34738..35811
                     /locus_tag="ACHWN2_001022"
     CDS             34738..35811
                     /locus_tag="ACHWN2_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR8930655.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35939..39385
                     /gene="mfd"
                     /locus_tag="ACHWN2_001023"
     CDS             35939..39385
                     /gene="mfd"
                     /locus_tag="ACHWN2_001023"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR8930656.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39528..40490
                     /gene="ldtC"
                     /locus_tag="ACHWN2_001024"
     CDS             39528..40490
                     /gene="ldtC"
                     /locus_tag="ACHWN2_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFR8930657.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40573..40785)
                     /locus_tag="ACHWN2_001025"
     CDS             complement(40573..40785)
                     /locus_tag="ACHWN2_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFR8930658.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         9649 a        10801 c        10111 g        10395 t
ORIGIN      
        1 atgctcgata tgctattaat aatatccaca taaataaaac aacgggcgtg ttatacgccc
       61 gtttaaatat ttaacacatg tagtgattac atattcttga tgatcgcatc accaaactct
      121 gaacatttca gcagtttagc gccttccatc agacgctcga agtcataggt tacggtcttg
      181 gcgttgatcg cgccttccat acctttaaca atcaggtctg cggcttcagt ccagcccata
      241 tggcgtaaca tcatctcagc ggagagaata atagagccag ggtttacttt gtcctgaccg
      301 gcgtatttcg gcgcagtacc gtgggtggct tcaaacaggg cgcattcgtc accgatgttt
      361 gctccagggg cgataccgat accgccaacc tgcgctgcca gagcgtcaga aatgtagtca
      421 ccgttcaggt tcatacaggc gataacatca tattcagccg gacgcagcag gatctgttgc
      481 aggaatgcat cagcaatcac gtctttaatg acgatctctt tgccggtatt cgggttttta
      541 actttcagcc acgggccgcc gtcgatcagt tcaccgccaa attcttcacg cgccagctgg
      601 tagccccagt ctttaaacgc gccttcggtg aacttcatga tgttgccttt gtgcaccagg
      661 gtcacagagt cacgatcgtt agcaattgcg tattcgatcg ctgcacgaac cagacgtttg
      721 gtgccttctt cagaacacgg cttaataccg ataccgcaat gttccgggaa gcgaattttc
      781 ttcacgccca tctcttcacg caggaattta atcactttct cggcgtcggc agagtcagct
      841 ttccattcga tacccgcata aatgtcttcc gagttttcac ggaagataac catatcggtc
      901 agttcagggt gtttaaccgg gcttggagtg ccctgatagt aacgtaccgg acgcaggcag
      961 atgtagagat ccagttcctg acgcagggca acgttgagag aacgaatacc gccaccaacc
     1021 ggagtggtca gcgggccttt aatggcaacg cgatattcac gaatcagatc aagggtttca
     1081 gcaggcagcc agacatcctg accataaacc tgtgtggatt tttcaccggt gtaaatttcc
     1141 atccaggaga ttttacgctc gcctttatag gctttctcga ctgcagcgtc gaccactttc
     1201 agcatggctg gggttacatc tacaccgatt ccatcacctt caatgtaagg gataatcgga
     1261 ttttcaggaa cgttgagttt gccgttttgc agggtgatct tcttgccttg tgccggaaca
     1321 actactttac tttccattca cctctccttc gagcgctact ggtttgctcg tctgccacca
     1381 cgttgcatat gcgtttgcgt cctgcgatac ggatgcttta gagcaatttt ttgttaatga
     1441 tttgtaattg acttgtcaat actaccctat tgtttgccgt catgaaagat gcgcgttatt
     1501 aggctataat gcggcaattc ataatctctg aaaataccat gcaaaaaact tcttttagaa
     1561 atcaccaggt taagcgattc agctcgcaac gttctaccag gcgtaaacat gaaaaccagc
     1621 ccacgcgtgt gatcctcttc aataaaccct acgatgttct tccgcagttc accgatgaaa
     1681 ccggacgcaa aacattaaaa gaattcatcc cggttcaggg cgtttatgca gcaggtcgcc
     1741 ttgaccgcga tagcgaaggg ttgctggtgc tgaccaataa cggcgcgttg caggcgcgtt
     1801 taacccagcc gggtaaacgc accggaaaaa tctattatgt gcaggtggaa ggtattccca
     1861 cacaagacgc acttgaagcc ttgcgcaatg gcgtaacctt aaatgatggc cctaccctgc
     1921 ccgcaggcgc ggagctggtt gacgaacccg cgtggttatg gccgcggaat ccaccaattc
     1981 gtgaacgcaa aagtattccc accagctggc tgaagatcac cttatatgaa ggacgtaatc
     2041 gccaggtgcg ccgcatgacc gcccatgttg gtttccccac gctgcgactg attcgctatg
     2101 cgatgggaga ttactctttg gataatcttg ccaatggcga atggcgagaa gtgacagatt
     2161 aaggatgcaa aatgtttaaa ccgcacgtta ccgttgcttg cgtggtgcac gcagaaggca
     2221 aatttttagt cgttgaagag acgattaatg gtaaagcgtt atggaaccaa cctgccgggc
     2281 atctggaagc cgatgaaacc ttagtggaag ccgccgcccg tgaactgtgg gaagaaaccg
     2341 gcatcagcgc gcagccgcaa cactttattc gtatgcatca gtggattgcg ccagataaaa
     2401 cgccgttttt gcgtttcctc tttgccattg agcttgagca aatatgcccg acccagcctc
     2461 atgacagcga tatcgactgc tgccgttggg tcagcgccga agaaatttta caggcgtcaa
     2521 atcttcgttc gccgctggtg gcggaaagta ttcgttgtta tcaaagcggg caacgttatc
     2581 cgctggagat gattggcgat tttaactggc cttttacaaa gggtgtcatc taaaaggctg
     2641 cgtgatagaa tacgccgcct tgaagttcaa tgtcgtgagt gatccaatgt ctgaaaccgc
     2701 aaaaaaagta atcgtcggca tgtccggcgg tgtcgattcc tccgtttctg cctggctgtt
     2761 gcaacaacag ggatatcagg tcgaaggcct gtttatgaag aactgggaag aagacgacgg
     2821 tgaggaatat tgcacagcag cagcggatct ggctgatgcc caggcggtct gcgacaagct
     2881 cggcattgaa ctgcacaccg ttaactttgc tgccgagtac tgggacaacg tcttcgaact
     2941 gttccttgcc gaatataaag ccggtcgcac gccgaatccg gatattctgt gcaacaagga
     3001 gatcaaattt aaagccttcc tcgaatttgc cgccgaagat ttaggtgccg attatatcgc
     3061 taccggtcat tacgtacgtc gggccgatgt cgatggcaag agccgcctgc tgcgtggtct
     3121 ggacagcaat aaagaccaga gctacttcct ttatacgctc agccatgagc agattgcgca
     3181 aagcctgttc ccggtaggcg aactggaaaa accgcaggtg cgtaagattg ctgaagatct
     3241 tggtctggtc accgcgaaga aaaaagactc taccggcatt tgcttcattg gcgaacgtaa
     3301 attccgcgag ttcctgggcc gttatctccc ggcgcaaccg ggcaaaatca ttaccgtcga
     3361 tggcgatgaa attggtgagc accaggggct gatgtatcac actctcggtc agcgtaaagg
     3421 tctgggtatc ggtggcacca aagaaggtac cgaagaaccg tggtatgtgg tggacaaaga
     3481 cgtcgaaaac aacattctgg ttgtcgctca gggccatgaa cacccgcggc tgatgtctgt
     3541 cgggttgatt gcccagcagc tgcactgggt cgatcgcgaa ccattcaccg gcactatgcg
     3601 ttgcacggta aaaacccgct atcgccagac cgacattccg tgcaccgtca aggcgctgga
     3661 cgacgatcgc attaaagtga ttttcgatga gccggttgcc gccgtgacgc cgggccagtc
     3721 tgccgtcttc tataacggtg aagtgtgcct cggtggcggt attattgagc agcgtctgcc
     3781 gctgccggtc tgattattat ctttacttaa aacaaggaag cagtgaacgt ggcaaagaat
     3841 tactatgaca tcaccctcgc cctggccggt atttgtcagt cggcacgcct ggtgcaacaa
     3901 ctcgctcacc aggggcattg tgatgccgat gcgctacacg tctcactcaa cagtattatt
     3961 gatatgaacc ccagctcgac gctggcggtt tttggcggta gcgaagccaa cctgcgcgtc
     4021 gggctggaaa ccctgctcgg cgtgctcaat gccagcagtt gccagggctt aaacgccgaa
     4081 ttaacccgct acacactcag cttgatggtg cttgagcgca aactctcctc agcgaaaggc
     4141 gcgctcgaca ctctgggcaa ccggatcaac ggcctgcaac gccagctcga acacttcgat
     4201 ttacagtccg aaacgctgat gagcgcgatg gctgctatct atgttgatgt gatcagcccg
     4261 cttggcccgc gcattcaggt caccggttcc cctgctgtac tgcaaagccc acaagtgcag
     4321 gcgaaagttc gcgcaaccct gctggcaggc attcgcgccg ccgtgctctg gcaccaggtc
     4381 ggcggcggac gtctacaact gatgttttct cgtaatcgcc tgaccactca ggcaaaacaa
     4441 attcttgctc atttaacccc ggagttgtga tctatggaat tatcctcact gaccgccgtt
     4501 tcccctgtcg atggacgcta cggcgataaa gtcagcgcgc tgcgcgggat tttcagcgaa
     4561 tatggtttgc tgaaattccg tgtacaagtt gaagtacgtt ggctgcaaaa actggccgcg
     4621 cacgcagcga tcaaggaagt tcctgctttt gctgccgacg caatcggtta ccttgatgca
     4681 attgtcgcca atttcagcga agaagatgcc gcacgcatca aaaccatcga gcgtaccact
     4741 aaccacgacg ttaaagcggt tgagtatttc ctgaaagaaa aagtggcgga gatcccggaa
     4801 ctgcacgcgg tttctgaatt catccacttt gcctgtactt cggaagatat caataacctc
     4861 tcccacgcat taatgctgaa aaccgcgcgt gatgaagtga tcctgccgta ctggcgtcaa
     4921 ctgattgatg gcattaaaga tctcgccgtt cagtaccgcg atatcccgct gctgtctcgt
     4981 acccacggtc agccagccac gccgtcaacc atcggtaaag agatggcaaa cgtcgcctac
     5041 cgtatggagc gccagcaccg ccagcttaac caggtggaga tcctcggcaa aatcaacggc
     5101 gcggtcggta actataacgc ccacatcgcc gcttacccgg aagttgactg gcatcagttc
     5161 agcgaagagt tcgtcacctc gctgggtatt caatggaacc cgtacactac ccagattgaa
     5221 ccgcacgact acattgccga actgtttgat tgcgttgcgc gcttcaacac cattctgatc
     5281 gactttgacc gtgacgtctg gggttatatc gcccttaacc acttcaaaca gaaaaccatt
     5341 gctggtgaga ttggttcttc caccatgccg cataaagtta acccgatcga cttcgaaaac
     5401 tccgaaggga atctgggcct ttccaacgcg gtattgcagc atctggcaag caaactgccg
     5461 gtttcccgct ggcagcgtga cctgaccgac tccaccgtgc tgcgtaacct cggcgtgggt
     5521 atcggttatg cgctgattgc gtatcaatcc accctgaaag gcgtgagcaa actggaagtg
     5581 aaccgtgacc atctgctgga tgagctggat cacaactggg aagtgctggc agaaccaatc
     5641 cagacagtta tgcgtcgcta tggcatcgaa aaaccgtacg agaagctgaa agagcttact
     5701 cgcggtaagc gcgttgacgc cgaaggcatg aagcagttta tcgatggtct ggcgttgcca
     5761 gaagaagaga aagcccgcct gaaagcgatg acgccggcta actatattgg tcgagctatc
     5821 acgatggttg atgagctgaa ataagcctca aatcagtgcc ggatggcgat gctgtccggc
     5881 ctgcttatta agattatcca ctttttcttt tttcacttta cctcccctcc ccgctggttt
     5941 atttaatgtt tacccccata aacacataat cgcgttacac tattttaata attaagacag
     6001 ggagaaataa aaatgcgcgt actggttgtt gaagacaatg cgttgttacg tcaccacctt
     6061 aaagttcaga ttcaggatgc tggtcatcag gtcgatgacg cagaagatgc caaagaagcc
     6121 gattattatc tcaatgaaca tttaccggat attgcgattg tcgatctcgg attgcctgac
     6181 gaggacggtc tgtcactgat tcgccgctgg cgtagcaacg atgtttcact gccgattctg
     6241 gtattaaccg cccgtgaaag ctggcaggac aaagtcgaag tattaagtgc cggtgctgat
     6301 gattacgtga ctaaaccgtt tcatattgaa gaggtgatgg cgcgaatgca ggcattaatg
     6361 cggcgtaata gcggtctggc ttcacaggtc atttcgctcc ccccgtttca ggttgatctc
     6421 tctcgccgtg aattatctat taatgacgaa gtgatcaaac tgaccgcgtt cgaatacacc
     6481 attatggaaa cgttgatacg caataatggc aaagtggtca gcaaagattc gttaatgcta
     6541 caactctatc cggatgcgga gctgcgggaa agccatacca ttgatgtact gatgggacgt
     6601 ctgcgcaaaa aaattcaggc acaatatccg caagaagtca ttaccaccgt tcgcggccag
     6661 ggctacctgt tcgaattgcg ctgatgaaaa aattactgcg tctttttttc ccgctctcgc
     6721 tgcgggtacg ttttctgttg gcaacggcag cggtagtact ggtgctttcg cttgcctacg
     6781 gaatggtcgc gctgatcggt tatagcgtca gtttcgataa aactacgttt cggctgttac
     6841 gtggcgagag caatctgttc tatacccttg cgcagtggga aaacaataag ttgcatgtcg
     6901 agttacccga aaatatcgac aagcaaagcc ccaccatgac gctaatttat gatgagaacg
     6961 ggcagctttt atgggcgcaa cgtgacgtgc cctggctgat gaaaatgatc cagcctgact
     7021 ggctgaaatc gaatggtttt catgaaattg aagcggatgt taacgatacc agcctcttgc
     7081 tgagtggaga tcattcgata cagcaacagt tgcaggaagt gcgggaagat gatgacgacg
     7141 cggagatgac ccactcggtg gcggtaaacg tctacccggc aacatcgcgg atgccaaaat
     7201 taaccattgt ggtggtggat accattccgg tggagctaaa aagttcctat atggtctgga
     7261 gctggtttat ctatgtgctc tcagccaatc tgctgttagt gatcccgctg ctgtgggtcg
     7321 ccgcctggtg gagtttacgc cccatcgaag ccctggcaaa agaagtccgc gaactggaag
     7381 aacataaccg cgaattgctc aatccagcca caacgcgaga actgaccagt ctggtacgaa
     7441 acctgaaccg attgttaaaa agtgaacgcg aacgttacga caaataccgt acgacgctca
     7501 ccgacctgac ccatagtctg aaaacgccac tggcggtgct gcaaagtacg ctgcgttctc
     7561 tgcgtagtga aaagatgagc gtcagtgatg ctgagccggt aatgctggag caaatcagcc
     7621 gcatttcaca gcaaattggc tactacctgc atcgtgccag tatgcgcggc gggacattgc
     7681 tcagccgcga gctgcatccg gtcgccccac tgctggacaa tctcacctca gcgctgaaca
     7741 aagtgtatca acgcaaaggg gtcaatatct ctctcgatat ttcgccagag atcagctttg
     7801 tcggtgagca gaacgatttt gtcgaggtga tgggcaacgt gctggataat gcctgtaaat
     7861 attgcctcga gtttgtcgaa atttctgcaa ggcaaaccga cgagcatctc tatattgtgg
     7921 tcgaggatga tggccccggt attccattaa gcaagcgaga ggtcattttc gaccgtggtc
     7981 aacgggttga tactttacgc cctgggcaag gtgtagggct ggcggtagcc cgcgaaatca
     8041 ccgagcaata tgagggtaaa atcgtcgccg gagagagcat gctgggcggt gcgcggatgg
     8101 aggtgatttt tggtcgccag cattctgcgc cgaaagatga ataaatatgt ccttttacca
     8161 ctacatcaag tcattgattc gatcaaacaa ttttcattga aaaagtgcca aaaatcgcaa
     8221 attgactaca ccattaacta caccgttcgg tgcactgtat gaaacaacgt ggaacaaata
     8281 gacacaagaa atatacaggc gggtaatctt tccaggggga agcgcaccaa ttcatgaggg
     8341 gcgttaatgt cgatatgggg atccccataa cggggctacc ggattttttt tccggttaac
     8401 ctttaatcag gctggtgggt tttacatacc cttgatcacc gtaatgatga tccagatgtg
     8461 caccttttcc accaatggcg gaaaagctcc aaccgtaacg atgatcgcta tggttgcctc
     8521 gatggtctgc gtggtcatta tgaccgttca gccagtgacc ttaatcagaa tttttcgcta
     8581 aaaaatgacg ttggccacgt catccggaaa gcacaaaatc acgtcgattt ttaatgaacg
     8641 gttaaaccgt ctttttgtca cggtgtgtca ctggttgtca ctctccgtca cgattgaggt
     8701 ggtggtcatt ctgcccgacc gcgcatcttt gcgctatcgg gaatatcaaa cagttacctc
     8761 gccgacttcc tcaaattgag gtttccgaaa atatcagagc gggttagcga ttaagctgac
     8821 gagtaattaa actgcgaaaa tttcgtagtt tctgaatata ttccggcctg ctggttaaca
     8881 gtcctcagat gtgagggatg tagccagcgc aattttgcgc tttgctttaa tctcagtgag
     8941 ttaccgccgt aaccgctccg gcttcttcca gtggtacgtg attttctcct tctcccgata
     9001 cagttcaacg cggcgattgt aggccagcat ttccagaacg cggatccgta tgtcgcgcat
     9061 atccgcatca ttaagctgga taccatcacg gcgcatcacc tcagcaacaa cacgcgcata
     9121 attttcggct gtcacgctgt ccggctgcgt ggtctgttcg tcagcctgct ggctgattcc
     9181 agagacgcgg cggattaatc gcagtatttc ggcttctgtc atgctgctga cctcattaca
     9241 ccccgctaaa ttcttccagt ttctggcggt ggctgtcgct tatatcaaaa gcaaaatcct
     9301 catgctctgc ctggaatgta ccaaacgcca tcagcgccgc tacgctcggg tctatcttgt
     9361 tcggtgattt tttcttgttc ggcttgatat tggcgttcgc gtcactctgc atcacgacgt
     9421 tactcatcga ccaggacaac accggatcgc cacgatgcac aatcaccctg cggttaacaa
     9481 aaacttcgaa cgatttcgcc gccggactga atctgaggta ggtttgcggg aacggctcca
     9541 cctcaaaacc agccccctgt aattgcgtcc tgagatgcgt ggcgttccac gtatcaaagc
     9601 ccaccagcct gatattaaat ttctccgcat cctgcatgat gtcatctctg atccggtcgt
     9661 aatcaatgca gtcacctggc gttgtgcgta tccagcccgc tttagcccac tggcgataga
     9721 cagcgcggtt tttattggcg gggttctgta gctggaactc cggcagataa tgacgggaaa
     9781 tcagcataat ggttttaccg accggaaagg catagcatac gctggaaata tcgctggttg
     9841 atgataagtc cagccccgcg taacactcct gaccgtgtaa atcttcctcc gtgaacgttc
     9901 cggcacactc agcccatgca ccattaccca tccacggggt agtcccctga caccagatat
     9961 taaatcgctt tgtcatcatc tccacccact gcgacggaat accccgcgct ttctggatgg
    10021 ttgaggccag tttttcacga tccacggaaa catcaatatt agggttcgcc tttatccaca
    10081 tcgccggatc atcaacctcg ttttcgtcgt ccagttcgta aatcagtaca aaaattgaat
    10141 cgttaacctc ttcgccgtcc aggatctggc agcaataatc atagtgctgt ttacaggctg
    10201 aaacgacgtt actgcccgat gtggtaatgg caaataacag cccttcggga cgtgcgccca
    10261 tccccagctc aagcgcggaa taaacgccgt tatcggggtg taggtggtat tcgtccacga
    10321 tggaaagact ggggtttgtc ccctcgatgg ttgccgcttt tgctgccagc ggctttaaca
    10381 ggctgttgct tttcgggtgt atcaccttat gcgcctgaat attcacccgc ctgcgtaacg
    10441 gtcgggataa aaggcacatc tgacgcgcat tatcaaacac gatccgcgcc tggtcacggc
    10501 tcaccgctgc ggtgtagata tcctgctgac cgttctccat aatcagaaac cagttagcca
    10561 gaatcgcggc ggtcgtggat ttcgcatttt tgcgcggcac ttcgataaag gcgctcgtgt
    10621 atttgcgccg tccggtggcc ttaaccttaa agccgaggat gcacgcaaag gcgaactgct
    10681 gccacggctc cagctcaatg ggtctgccac gcatcggtcc ttttacgtgc gggcacaccc
    10741 tggaaaaggc aataaaccgc tccacaacct cacgatcgaa cgtgtaaagg gggcttttaa
    10801 ggtccgaaaa gtaccgttta acagcctgtt ttaaccgttt acaggccgga attttgcccg
    10861 tttttacgtc ttctgcgtac ttattccagg cggtcaagct cgtcctcttc ttctgtttcc
    10921 ggtggatttt tacggcggct tatcgggtca aaaccgagca aggaggccat ttttatcatc
    10981 actctttcag cgtcggattt tgcgcttaat gcggggtttc tgctctcgcc gccctgactg
    11041 ttaacaatgc tgaacccgcg cgccgcaagg tctgcaacgg ctttccggta aatggagtag
    11101 ttgacgcaat acagttccag attgctccag tcggcggggg tcaggtctcc ccgttccgca
    11161 agctgccgcg atttttcccg ccactgcttc acggcgatat catccaggta ggcggggtcg
    11221 ggtttgtcct gaaaacgccc cccaccccgt catacagcct catcagcgat tgcggaaaca
    11281 ttccataacc tcgcggtcac ggtcggttaa tcgcttcgct gtgatgcgtt ctgcgcgtcc
    11341 tgacgcttta tctttatgcc ctgtttcctg tgtcttccat gcgtcacgct gccttataag
    11401 tccacggata aggcggtttt gttcccgctc attcatcagc gtcatacatc cagttattgc
    11461 ggttagcagc ccgttcttcc tcttcacgaa atccacctgc ggcacgcttg ctttttgttg
    11521 ccggatcaag ccatttcgtt ttctggttat gacacgcctg gcacaatggt tgatggttcc
    11581 attcgggcca gaagagaaca tcatcaccgc cattaatcgg gataatgtga tccaccacca
    11641 cggcgggcgt atatatcccc ttctcaaggc atcgcacgca taacgggttt ttactcagat
    11701 acatggcgcg gtatttgtcc cactgtctgg aatatccacg cgcgcggcgg tgtcctcgtc
    11761 tggcatcctc tgcacgccat gccgcccgcc tgtgctcctc acacttgccg gatttaacgc
    11821 gcctgttaca gcctggttct gtgcatcgtc ttaatggttg ccacggcatc agtacccccc
    11881 cacatcacgg taagccgtcc agagtgcgcc tatcgtcatg ggtacgcgtg tttttgcgtt
    11941 atccgcgaca atctggcgat tttcatacag gtgagcgata aacatcatgc agccaatctt
    12001 tatggctggc gtgaacgcca gcccgtcatc aaagcgcctg cctatgtgta tctggcacgc
    12061 ctcaagcgat gcggcaatgt atccgctgat taactggtct tcatcgtcgc catcgatgcg
    12121 gcaatggagt ttcacttctt ccagggtgat aagttcttct gtcatttttc cgcgccctca
    12181 cgacaaagaa tttcaaggcg tgtcctggcg gcatccggca gcggctgccc gatgatattc
    12241 agtacacgcc ccgccagcgg ccccgtactg acctttatcc ggctggtggc gttgatgtcc
    12301 ttccggtacc gtatccagat ccttacggta ccaatcgcca gctctgcgcc cgatgaaatg
    12361 gcctccttgc tgctgaccat gttcacgctt gcccagagtg tgtgaccgtc ctcccacgtt
    12421 tcgagtattt cgccggttat atccctggtc tgtttcaggg tctgaatcgt tatcctgtca
    12481 cgcagtcgcc ctatgttcat tccggttttt ctccctcgct gatttttact tcctgtttcc
    12541 atgcctggct gaactcatca ccaccatcac gcggtgacag cccttcacgt tcgcgggctt
    12601 cgttcggaca cataacgtcg gattttatgc cgcgctcata ggtggcaaag cgttcgccag
    12661 gtgtggcccg taacaggtcc gcgctgtcaa actccacctg ataccggata ccaggtacag
    12721 gcgaggccac cagcaaggcg ttttttatct gctgctcaaa gttcgccagc cacgggcgca
    12781 tcgtcatggt gagaaatgcg cggcttgcct cgctgaagtt gctgtaggtg ctgttgctgt
    12841 attcctgcaa aaaaatcggc gagacgttga acattcgggc gatgtcttca atggtgaagc
    12901 gacgggaggc cagccattcg gcatcctgat tactcatgcc cagctgctga tagctcatcc
    12961 ccccttcaag gatgggcgtt tttccggcgt ttctggcccc tttgtagcgt tccagtgcgg
    13021 ctaatgcctg tttgcccttc acgccgtcca gccattcgcc tgacgtgata acccctgccg
    13081 ccatcatgcc atcacgcatc acgctcgcgc cgtggcgttg ttgggccagc ccaagcccca
    13141 gcgattcgcg gcagatggtt acaggtgagc gccccataaa accgtcatcc gtggagtaac
    13201 gaaggtggag aacctcccac ggtaaatagt tgcggatgtt tccggtgtag gcgtcagtga
    13261 tgcagtagcg ccagttatga tcacctgtct gctccacgtt caccgactgc ggcgggtaag
    13321 gatgtaaagc cgccggaaaa ccatcacgcc cccactgaat aaccgcataa gcattaccac
    13381 ttaacaggca gtagcggatc atcattcgct taaactggta gggtgtctgc cacgcgttcg
    13441 ggcgctcgtt gaggatgtga tcgaccggat gagaatcaag ccattcgcgg gcctccttcc
    13501 ccttctcatt gcgtaccagg tacaggtaac acggcatggt agccaccgcg tcagagatga
    13561 ccgtgacggc gttcatgacg gcggggagtg attccgctgt cccagatgat acgtactcac
    13621 ccgcccccgt gttcgatgtg ccggccagcg ccataaattc atcaagcgtc atgctgcgct
    13681 gctctttttt tcgtctgaaa ggccacatat cacacccccg ctaaatccgc ccaccagcgg
    13741 cgattatccg cacgcggcat tttttcgggg tgctgctcat acagggaacg gcgggccagc
    13801 tccacgccgg aatcggggta agccggtacc gatgtaacgg taatttcgta cagttccgcc
    13861 accagcacgg tgcgcacgca tggatctgtt gtggtatccc atacatcctt acgggagcga
    13921 aagccaaagc tcatccccga gatgtcccca cgcttaacca gttcgataac gtcgcgccct
    13981 gtactggtat ccggtggggt cagttcaaag cgtaaccctg tttcgtcctc ttccagtttc
    14041 agcgtgccgg aacgggtgcg ccccagtaac atgctgtggt catgctcata caggccgcga
    14101 acgtcattac ccgccgcaag ccattcagta aacgcccccc gctggaattt ttcgtaaaac
    14161 tcaccccata acagttctga aagcttatcc cagcgaacaa cgtagcccgt cagcgtaccg
    14221 gctccgctgg tggtgatttc cgatgaccgg atttccatac tcttcataat tttttcaccc
    14281 ataaaacact aaaggggctt tttagcccct tctgtatgct gttaatcgtc gtcttccggc
    14341 agtgccagaa tcttgatcgc gttcgaatcc accacgccac cgcctaaata tttttgcgtg
    14401 aaaattttga tgaatcctgg ttctgtcagg ttgtccggtc tggtgcgaac acctgtttcg
    14461 tgatcaacaa tgtagtaacc gcgtttgaag tcacccaggg caataacgtt atcaggcata
    14521 aactccagat attcgaccgg aaggcccagc aacgtatcag gatcacccgc ctgtaaacgg
    14581 tcgcgccaga tataatcacc gttcgcgttc ttcaccttct gaagttttgc cgccgtcgtg
    14641 gagttaacca ctcagaccgc gtttttgcgg tattttttac gtaatgcaaa tttcaggtcg
    14701 atcagcgggt ccgcagatgt ccacgccaga gattcggaag gtttaattac ctgcaacgta
    14761 ccaaaatcac gctctttgtc gttcttctct gcacggggta cggataaaaa accttttgct
    14821 tttttgtcac cgtcgcccac aaccagatcg ctttcttcgg tttccgtgaa ggtgtcgcca
    14881 atctcacccg tcagccatga aaggatgtcc acatcggaaa aatccacgat ttcctgtgtg
    14941 gtgcgcgggt acgcatagac cggatacagc ttaatgctca cctcgttaat ctgcggggtg
    15001 ctggtctgtt cgcgtgcctt accctcttca ccgtggttaa cggtcgcacc gccagcggaa
    15061 acaagctgct taaactcgtt gctgctgatt ttcttcacgg tacagatgcg acgcatggtg
    15121 gattcatccg ccagcattcg catgatttcg gtgttcagtt cagggataac ggtataacca
    15181 ccatcagcgg gaacgcctgt acttaatgcg cgggtttcac cggtcagaat gtagttacgt
    15241 agttctgcgg ggtcagtggt ctggctgctt ttacctggct tgctgcgctc ttcgtctgca
    15301 atggcttcaa ggcgggaaat gtctttatcg agggattcag ctttagcgcg taattcgtca
    15361 aattttgcgc cctcagcatc gttaagactg cggttttctt tttcagcgtt ctccagcatg
    15421 tcgcgcatct gatttttaat ggcggttttc tgctggcgta attcgattat tctctgcata
    15481 aaaaaagtcc tggggttaag taaggaactc caggacgcgg caaacactca accgtttttc
    15541 ataaggaaat cagtaatcgc accgatcgtt ttcccgcctg gtaatgaata ttggcgagca
    15601 cattaacagg cgggaaagtg gccccagcgt cctggcacca cgggcgagaa taatcatgat
    15661 tcagttcggg taaaatatgc cgatccgctc agtgaacaac gtggaacaac cacgaacaaa
    15721 taatttacaa aaaataacaa aaagccggat tgctccggct gtatgtgctg tatgggtgct
    15781 cagtctctga tccctttcag aaattctatc aatgcatctg tctgttcagg atttactgcc
    15841 agcatttcac cggatagcgc gcaacgaaca aaaccatgat gatccttttc aatcagcgcc
    15901 cccgtttcca gaaatgcgcg gtaatcagtg atattcatcg tctccatgtt gtcagcatgg
    15961 tatttatcac gctgtttaag tatctcatca aatttcatcg gcattgcgtt ttcctctgtt
    16021 gtgtctgttt gtttcagata gtaactatgc cggaccgtga cgaaaagcca gtagtgctcc
    16081 ataccgtttc aaatgacgca aaaaaagccg gattgctccg gctgcgtggt gtggttctga
    16141 tattcctact ctgctaattt gctcagcccc atgtctgcgt aagtgcgatt tactgcattt
    16201 ctcaggtctg cgtagttctc cgttggcggc tccggtgtgc ccttgctgct ctgttttacc
    16261 agcgggtcgc ccaccagctg cccgtaagct gcaatttgtg ctgtcatatt gccacctcag
    16321 tgaagcgatc cggcattgtt ctttttcatc tcttccatca ttttttcagt gagcattacg
    16381 cactgaaacc ccatgttttt gatgtctacg ggtttcatgc ctttgtcagt ggatatcaca
    16441 ggcagaatat cctgatacac atcgcggtca gggtaactaa gcacacaaag ctcccatgat
    16501 gccacgggtg aggatccttc gtctgtttca acccaccagc ctggtgtagc tgcgcttatt
    16561 tgcagaatcg tgatccgtcc gttgtgcttg taaaattttt caccaaattt tggttcgctc
    16621 atgattccac ctctccggtt ttaacgttga ttgttgttac ctgttccgct tcggcaatct
    16681 cccgctctgt cagcgtggca aaatttgccg ccgtcgtggt catgaatgcg cttatcagtt
    16741 cgggatgtgc tttcgcgtat ccttcccccg cgtggcggtc tatcgttctg attgccacct
    16801 ttaaagcgtg ctctgtcatg tctaacgcgc gatatttcgg ttctgttctg tctctgcgtt
    16861 ttttgaatga tttattaaat ttccctgaag tgtgcatatt tatttttacc ccctcgttta
    16921 aaaagttttg agttgtgcct ccccttgtct accttatcta ccttagtggc cctcatgcca
    16981 gtaatggcgc tgctttcagc ggggtagagt gcttttatcc actatctacc ccgtgtctac
    17041 ctccctgtct gattcaggta aaatcaggta gagagggtag atagtgggta gacagtaaaa
    17101 aaaggctatc tacctaactt aatgcactga attaaatgta tttttcttta ctcaggtaga
    17161 cagggtagac agcaattaca aaaaattata aaaacgcgtc gcactcgtct gttgttattg
    17221 cgttagtctg cgttactccc ttaactttcc gcgtaatata ttcatgtccg taaactttcg
    17281 cggctggctt catagccttg ccaaagtcat ttacgtttag cggtttgctc ctgcctgcgt
    17341 acgccataaa cgccagatag acgcggtaaa ggctgtttct ggtcgtgtac ttcactgaat
    17401 cgccaccgcc gcccatcatc aggccgcgtg cttcctccag aaaattcagg aactggcaaa
    17461 actcaataac cggatccgtc tgttgcttta ttgccagagc ttcatcaccg tcacgctgtt
    17521 ccagtagtaa agcccgtgct ttctcagagt cggtaaagtt cgccagcaat cggcggataa
    17581 tgacagggat ttcagccgca atcttttccg gtagctccct gtctttttcg gcctcactaa
    17641 cgatattgtc gaaacggaaa atcacgcgac gacgtgccac acctccggcc cgttcggtga
    17701 atatcatcgg gttattgttg gtcgccagca ccaccgccct gattacagcc gtgaaacgct
    17761 tttcgtattt cgggttaatt tccacggggt caccgcccgt gattttcttg atgcccgttc
    17821 cttcgcctgt atatttcggc tggtcagcca ggacgataag acgactcccg acaacctgcg
    17881 cacgtccacc agcatcatca agcgatgtca tttcagcgct taccgtgttc tgtttccctg
    17941 ccagaaggct ggctatgtgt gtgaatgtac ttttaccgct cccgccgtct ccggtggcct
    18001 caataaacat ctgccagtcg taccggttcg ccataatcat gtacagcgcg gcacatatac
    18061 gcatcatctt gcgctggtct tttccggctg cgtgctcaag ccatttatga aagtttagcg
    18121 cgttatcgcg gatgttctcc cctggtgctg gtggcgtgta ctcaatgccg ttgtgcgtgg
    18181 tgatccagtt ctccggcgtg tgcggggaaa attcccccgt tttcaggtca agcgcaccat
    18241 tggcgaacgg cagcaaatcg ccggacggct cgcccattgg ttcggcaata acttttaacg
    18301 cttccacggc gttattgatt acgcgcttgc tgaaagtggc cctgtgctct gaatagatca
    18361 ccaccatttc gcggctaagt tccattgtgc tgaccggaca ccataccccg ccgcgccata
    18421 cgtgaacgat ttcactttcc tgatgcacac aaacgccatc aaagcgctcg gcaagcagct
    18481 gcgcgcgctc actgtctgcc atttgtgcca gttgcgtatt tttctgctcc ggcatggtaa
    18541 gccctgcggc aatattttca cgctcagcat tcagatagcg ccgccagttt tcacacttct
    18601 ggacgtgcat tccttcgggg taaaaatttg cgtcctgaat atctgccgcc gccagcttct
    18661 gaccaatctt tttggtctcc accaaatcca gttctccggc ctggtacagc cttacgcgtt
    18721 tttttccatc cggaacaatt ttcagtgcat caagttcggc aagctgattt ggcccaagcc
    18781 acacaggcgg cacattatcg ccggatgcgg ggccatcctg ctcctgccat tgtttcgcgt
    18841 gcgaccaggc atcactaccc gcaaagataa ttacctctgt gtctttgtgt tttattccgc
    18901 gtggctgttt ttttacgttc ggtgccagtt tcatttttta cccctgaata cgttaagcat
    18961 cttttttatt tcctgaatat tggcgcgtgc tttctccctg ctggttggtt tactgcgggg
    19021 cgctgcctgt accagcgtga aatcacgccg gaattgataa acaggcatca cgcaatcata
    19081 ttcgtaacct tcacggcggt aggtgatgcg ccgttctgcc acgcctttaa tcgttaccat
    19141 gccgccgtac tggtcgcggt aaatatcacc gcgcgtaaat ttagggtgag tgttgccact
    19201 ggcagttaag ccagaatatt taagtttcat tatttttatt ctccggtgtg ttgttcttta
    19261 tatctgtcgt gcaatagatc tatttcttgc agttccatta ttacaggctc aagaagcgtt
    19321 attaatgccg tggcaattct tgatttttgt ttgtcgcgtt cattgtcgcc aagtgtttca
    19381 agccatatgc gcaatatttc cagcatgttt tcactgtgag aaagtgcaag aaatgcgcgg
    19441 tctattgttt cgtggtaaat atcacgcata ttaatccccg tccgtcgttt ttcttaaaat
    19501 aacctctgtc acgaaatcag cataatcggc ggcgatattc agtacatcaa gcccgttgat
    19561 ttatattctc tcgtggaaag taagaaaaaa gccgctctaa taagttctgg cattgacgaa
    19621 agcgcatcag ccgcatcatc aggaacgccg gaaaattcct gtttcaggga attaaaacga
    19681 tcatcacgca taaccccccc cattttcaca atcggcaaca agaatatttt tagcgtcatt
    19741 cagtgaccgc gttgcgatgt atcggataca ttccagggcg aactgtgtcc ggtctttctg
    19801 ttcttctccc tgcgcaagct ctgccgtgca ttcaatatca ataagcgcgt gcatcagcgt
    19861 agtgagtgcg gcggctgctg cgttcggtgt ggttttattg tgcataggtt tccccctgac
    19921 gaatacgggc ggcgaataca gcaacacaac cggacgggca acggctacgc gcttcgcgtt
    19981 ccgtccaagc ggttacgtgg atgatttggg attctccggc actcagtgcc agaaaacgcc
    20041 acacaaaggc cgtttgtgtg tgtacaaggt gtggtatatg atttacggca accataacgg
    20101 ctcctagttt acgttgttgg ttagaagccc cgttactgct cctacagtgc ggggctttgc
    20161 tttttgtgtt gattgtgtgt aatgtgtcac tacacataaa tacattacct gaggtgtaat
    20221 taacgtgtca acacacaaaa acgaaaggcg tggtaatcct ccgttccagt tccgtttaga
    20281 tcctgactta cgggaaaaga tggaaaaggc acaacaacag gacggcgatg attctcttgc
    20341 tgcctggata aagcgaataa tccgcaaaga attacagcaa cgcggcatag aacccaaagg
    20401 ataaaaacta tcagcgccgt ggtgtgaggt actacggcgc attgctttac aggcgcacac
    20461 aatgaccaac gaagaatcaa ccgaaaaaac atcacctgca cgaaaaagac gacgcagaaa
    20521 gaaagagcat gaatcagaac gattcgcgcc gtgttctttt gcccttgaga agtaccttaa
    20581 agaatactca tcaaaggaga gaacttcgca gttatggcaa cgcactgaac cagactgatt
    20641 gcattgccca ccagcctgat agcggctatc atccccgtgc ttatgtttgg gatcacacac
    20701 acaaacggcg cagcgggtta tctgttcaga aaggcggctc catttcgggg ccgctttttt
    20761 tatgcctgaa aaaccccaat tttgtggttt tccagtttca ccagggcgaa cgaatccccg
    20821 cccacgttct ggcgtatatt caatcttcat ggttatagct ctgtgttcag atgattgatg
    20881 tgtggcggct gcgtgccgcc agcgtgatta atgaactgcc ttgcagctat ccttccaggc
    20941 cagaacctcg gataaagacc agccaacgga acgaccgcca agtttacgac gtgatgggaa
    21001 ttgtccggcc ttttccaggc ggtagcggca tgagcggcta aggcctgtta gcttttcgca
    21061 ttctttttca cgtataaacc gatcagtgct taacactatt gccccctttc gtttcttaaa
    21121 gagttatttc gtgttctatt gcgttgggat gtgtctgatt gtgtcaggat gattcagagt
    21181 tggcaaatgt tgaggccgta tggtttacag aaagaggaat aatcaggata aaatccttta
    21241 aattcatgtt aatacaaagg cataaaatat tgtttcatgc cttttttctc gctatttaaa
    21301 gagtgattcg ctagtgtata aaaaatcagt aacacattaa aaatcagcta cttataaatc
    21361 tgtacacttt ttcacctctt gttcgtagtt gttccgcatt gttgctcatt gttgcacgtt
    21421 gtatctgttc gcatatccag tatgcgcata ctgaaaaaac acgaaaaaaa ttattttctt
    21481 ctggctactg gtagcgtggt tacgttttca tgtgttcccg ccagtatccc taaccgctcc
    21541 gtccacatat ccagcgcatt gcgtttagca tccagataac gggaatgatt ataaactcgc
    21601 tgcattcctg gcatctggtg gcctgtaagc tgctccacga cgtgaggatc cacgcctaaa
    21661 tcgttcagca tcgttgtaaa ggtgcgccgg atgtcatgta gtgaccagtg agggtgattt
    21721 agcctcctgt gcgctaatct gccgtactgc gacacgcttg tttcctgttt cacttccccc
    21781 agcaataagc ccgtgtgcct gttctgctcc accagctgcg tgacgaacgg cagtattgct
    21841 tccggtatgg gccggaatat tgccaccttc gttttgctgt gttctttcgg cacggtccag
    21901 agcatttcgg taaaatccca ctcgctgatc tccgataacc tcagttctac cgtccggcat
    21961 ccgaacacaa tcaggaggcg gattaacgcg atgtagtagg gggagaatat ttttttgtcc
    22021 agtgcctgca ataattcgcc cagttctttg gtgcttaaga cacgctcgct tatatccggt
    22081 tttttcccaa cgtccgccac actcatatca tcaagaacgt tgctgattgc atagcgccgc
    22141 ctccggcaga acttaagcgc ctgtttgcac gtctgtagca agaatccggc agtaacaggc
    22201 gttcgctttg ccacctggtc aaaacaggcc agccagtgcc gtagctcgca tttatccagc
    22261 ggcatagcac caatgtgctg tattacgtga ttattaaggc gctttttcag ggcggcataa
    22321 tccacgcggt tttcctttgc gtacgactca agccagtagg tgagcgcatc gccaaccgtt
    22381 accggcttta acgcttcctg tacggtgtaa ttaagctcat gacgtggatt tttcccctct
    22441 gccagccatg cgcgacactg ggcggctttt tccctggctg atttcaggct cagatcagga
    22501 taatttccca gcttaatgcg ttccggtggt gccccccttc ccgttccggc cctgtaagtg
    22561 aaataccagg ttaaaaggcc actggttgaa tgcctgacgc tcaggtttcc accgtcatta
    22621 agaaaggctg ttttttgggc gggtgtgccg ttgattttcc tcagctgtgt atcgctcagt
    22681 ttgttaagtg ctctgctcat aattttgatt ccggtcatca caattaacta caccattaac
    22741 tacaccgatc gttgcacaaa gggctacaac gtgaaacaag ctggaacaag gaaaatcaca
    22801 aatctgttta taatcaaaaa cataatgaac aatctgaaac attatgaaac ggcaaaaaac
    22861 actaattgat aaaatatgtc catacttcac gcattacgtt aagcatccgt tataatcggt
    22921 tgcagatacc agcctgtgga tgcttaacat ggaataccaa ctcactctta actggcccga
    22981 ttttcttgaa cgtcactggc agaaacgccc ggtggtgtta aaacgcggct ttaataattt
    23041 tattgacccg atctctccag acgagttggc gggtctggcg atggaaagcg aagttgacag
    23101 tcgactggtc agtcaccagg atggcaaatg gcaggtcagc cacggcccgt tcgaaagcta
    23161 cgatcatctc ggtgaaacca actggtcatt actggtacag gcagtaaacc actggcatga
    23221 gccgaccgcc gcgctgatgc gaccgttccg tgaactaccg gactggcgta ttgatgatct
    23281 gatgatttct ttttctgtac ctggcggcgg cgtcggcccg catctcgatc agtacgacgt
    23341 gtttatcatt cagggtaccg gacgtcgtcg ctggcgagtg ggcgaaaagc tgcaaatgaa
    23401 acagcactgc ccacatccgg atctgttaca ggtcgatccg ttcgaagcca tcatcgatga
    23461 agagctggag cctggtgata ttctttatat tccgccagga ttcccgcatg aaggctacgc
    23521 gctggaaaat gcgatgaact attccgtggg ttttcgcgcg ccaaatacgc gggaattaat
    23581 tagcggattt gccgattatg tgctgcaacg tgaactgggc ggcaactact acagcgatcc
    23641 ggatgttcca cctcgtgctc atcctgcgga tgttctgccg caagagatgg ataaactgcg
    23701 tgagatgatg ctcgaattga tcaaccagcc ggaacacttt aagcaatggt ttggcgagtt
    23761 tatatcccag tcacgtcatg aactggatat cgcgccgccg gaaccgcctt atcagccaga
    23821 tgaaatctac gatgcgctga aacaaggtga tgtgctggtg cgcctgggtg gtctgcgcgt
    23881 attgcgcatt ggcgacgacg tgtatgccaa tggtgagaag atcgattccc cgcaccgtcc
    23941 ggcactggat gcactcgcca gcaacattgc gctgactgcg gagaattttg gcgatgcgct
    24001 ggaagatccg tcattcctcg cgatgctcgc ggcgctggtc aatagcgggt attggttctt
    24061 cgaagggtaa gtttggattt aagccggatg cagcatcaca tgccgcatcc cttttcgctt
    24121 acttccgttg cgccgttaac tcggcaatac ggacgatcac ctgcaccgct ttttccatac
    24181 cttccagaat cacaaactca tgcttaccat gatagttgta accgccagtg aacaggttcg
    24241 ggcaaggtaa tcccataaac gacaactgcg cgccgtcggt accgccgcgg atcggtttca
    24301 gttccggttc aatatcgcag tcacgcatcg cctgctgggc gatatcgaga atatgcggat
    24361 gctcaaccac tttctcgcgc atattgtagt aactgtcttc aatcaccaat tcaatgtagc
    24421 aatcaggatg taaccctttg cccacttttt tggcgatctc catcatttta cgtttacgcg
    24481 cttcaaactg tttacggtcg aaatcacgga tgatgtagtg catatcggcc cgttcaacgg
    24541 tgcctttcat gctcgccagg tgatagaaac cttcatagcc ttctgtcatt tccgggcttt
    24601 catccgccgg aacttccgca tgaatacgtg ccgccagcga cagcgcattt accatcactc
    24661 ctttcgccgt gcccggatga acattgttac cgacaatttt tatattgacc gatgcggcgt
    24721 tgaagttttc aaactccagt tcgcctacgc caccaccgtc aacagtgtaa gcccagcggg
    24781 catcgaaggc atcaacatca aaatgtttcg cccctttgcc cacttcttca tccggggtaa
    24841 aggcgacgcg aatatcacca tgcggaatgt ttttctgttg caataccgcc agcgcggtca
    24901 tgatttctgc aatacctgct ttgtcatcag cacctaacaa ggttttacca tcggtggtaa
    24961 tcaacgtctg acccagtagc tgatgcagca ccgggaacat aaccggtgat aaaacttcat
    25021 cgccgatacc cagcgcaata tcgccaccgc gatagttttc aacaatttgc ggattaacat
    25081 ttttgccgct gcaatccggt gaggtatcca catgagaaat aaagccaatc gccgggatat
    25141 cgccagggac gttagccggt aacgtcgcca tcaaagtgcc cttctcactt aaggtcacat
    25201 tgataagccc catttcttcg agctgctctt tcagcagatg caataacttc cattggcctt
    25261 ccgtgctggg aacctgtctc acccctgctt ttgattgggt atccagagac acgtagttca
    25321 aaaatcgctc aagtagttta tccatgtagt caccctcact ttttgtgaca acattattaa
    25381 gaagcaagaa aagacaaata ttgcgtcagg tcacttttac ccctgcaagc gggaatattt
    25441 atcagcatta ccttaatgaa tataaagcta agccagtaat tcacaacaaa gattggcgat
    25501 tcaagcggtt tgccgtagaa ttaccgccct cattaggagt caccaaggtg gttaaccaca
    25561 aaccccgcat cggtaagcca tccgttgcgt ttacatggga cagagtaaaa aattgaataa
    25621 acaaccgagt tcgctttcac cgctggtgca attggcggga attcgcaaat gctttgatgg
    25681 taaagaggtc attccccagc tggatctgac tatcaacaat ggcgagttcc tcacgctgct
    25741 tggcccttct ggctgcggta aaacaaccgt tcttcgcctg attgcaggtc tggaaactgt
    25801 tgattccgga cgcatcatgc tggataacga ggacatcacc cacgttccgg cggaaaaccg
    25861 ctatgtgaac actgttttcc aaagctatgc acttttcccc cacatgaccg tgttcgaaaa
    25921 tgtggccttt gggttgcgca tgcaaaaaac ccccgctgct gaaattacgc cccgcgtgat
    25981 ggaagccctg cggatggtgc agctggaaac cttcgctcaa cgtaaaccgc atcagctctc
    26041 tggtggtcaa cagcaacgcg tcgccattgc tcgagcggtg gttaacaagc ctcgtctgtt
    26101 gttgctggat gagtcgctct cagcgctgga ttacaaactg cgtaagcaaa tgcagaacga
    26161 gctgaaagcg ttacagcgta agcttggcat tacattcgtt ttcgtgactc acgaccagga
    26221 agaagcactc accatgtcgg acaggattgt ggtgatgcgc gatggtcgca ttgagcaaga
    26281 cggcacgccg cgtgaaatct acgaagagcc gaaaaacctg tttgttgccg ggttcattgg
    26341 cgaaattaat atgtttaacg ccacggtgat cgagcgactc gacgagcagc gcgtacgcgc
    26401 caacgttgaa ggccgcgaat gtaatatcta cgttaacttc gccgttgaac cggggcaaaa
    26461 actgcatgtt ctgctgcgcc cggaagactt acgtgttgaa gagattaacg acgacaacca
    26521 cgctgaaggg ctgattggtt acgttcgtga gcgtaactac aaaggcatga cgctggagtc
    26581 ggttgttgaa ctggaaaatg gcaagatggt gatggtcagc gaattcttca atgaagacga
    26641 tcctgacttt gaccactctc tcgaccaaaa aatggccatt aattgggtag aaagctggga
    26701 ggtcgtactg gctgatgaag aacacaagta agttccagaa tgtagtgatt gtcactattg
    26761 tcggttggct tgtgttgttt gtctttctgc ccaacctgat gatcattggc accagctttt
    26821 tgacccgcga cgacgccagt ttcgtcaaaa tggtctttac actggataac tacacgcgtc
    26881 tgctcgatcc gctctatttt gaagtgctat tgcactcgct gaatatggcg ctgatcgcca
    26941 ccctcgcctg cctggtgctg ggctatccat ttgcctggtt tctggcgaag ctgccacaca
    27001 aggtgcgtcc gctgctgctg tttctgctga ttgttccgtt ctggaccaac tcattgattc
    27061 gtatctacgg gctgaaaatt ttcctcagca ccaaaggcta tctcaacgag tttttgctct
    27121 ggctgggcgt tatcgacaca ccaatccgta tcatgttcac gcccagtgcg gtgattatcg
    27181 gtctggttta cattctgctg ccgtttatgg tgatgccgct gtactccagt atcgaaaaac
    27241 tggataagcc gttgcttgag gcggcgcgcg atctcggtgc cagcaagtta cagactttta
    27301 tccgcatcat tattccactg acgatgccgg ggattattgc cggatgtctg ctggtgatgc
    27361 tgccagcgat gggcctgttc tatgtgtccg acctgatggg cggtgcgaaa aacctgctga
    27421 tcggtaacgt catcaaggta cagttcctca atattcgtga ctggccgttt ggtgcagcca
    27481 ccagcattac gctgactatc gtaatgggcc tgatgttgct ggtttactgg cgcgcttctc
    27541 gtttgctgaa taagaaggtg gaactcgaat gatcggtcga ctgcttcgcg gcggttttat
    27601 gaccgctatc tacgcgtacc tgtatatccc aatcattatt ttgattgtga actcctttaa
    27661 cagctcgcgc tttggcatca actggcaggg ttttaccacc aaatggtata gcctgctgat
    27721 gaacaacgac agcctgttac aggcagcgca gcattcacta acaatggcgg tgttttcggc
    27781 gacgtttgct acgcttatcg gttcactgac ggcagttgcg ctgtaccgtt atcgctttcg
    27841 cggtaagccg ttcgttagcg gaatgctgtt tgtggtaatg atgtcaccag atatcgtgat
    27901 ggcaatttcg ctgctggtgc tgtttatgct gctgggtatt cagcttggct tctggtcgct
    27961 gctgttctcg catatcacct tctgcctgcc atttgtggtg gtgacggtgt attcgcgcct
    28021 gaaaggtttt gacgtgcgga tgctggaagc ggcgaaagat ctcggtgcca gcgaatttac
    28081 cattctgcga aaaatcattc tgccactggc aatgccagcg gtggcggcgg gctgggtgtt
    28141 aagctttacc ctgtcgatgg acgatgtggt agtttcttca ttcgtcaccg gaccaagtta
    28201 tgaaattctg ccgttaaaaa tttattcgat ggtcaaagtc ggcgtatcgc cggaagttaa
    28261 cgcgctggca accatattac tggtgctgtc gctggtgatg gttattgcca gccagcttat
    28321 tgctcgtgat aaaacgaaag gtaacacagg ggacgttaaa tgaaaaaatg gtcacgccac
    28381 ctgctcgcgg cgggtgctct ggcactgggc atgagcgccg ctcacgccga tgacaacaac
    28441 acgctgtatt tctacaactg gaccgagtac gtgccgccag gactgcttga acagttcacc
    28501 aaagaaaccg gtattaaggt tatctattcg acttacgagt cgaacgaaac catgtacgcc
    28561 aagctgaaaa cgtacaaaga cggtgcctat gacctggtgg ttccttcaac ctattacgtc
    28621 gataaaatgc gtaaagaggg gatgatccag aagatcgaca agtcgaagtt aacgaacttc
    28681 agcaatctcg atccagacat gctcaacaag ccttttgacc cgaataacga ctactccatt
    28741 ccgtatatct ggggtgcgac ggcgattggt gttaacggtg atgcggtgga tccgaaatct
    28801 gtcaccagct gggccgatct gtggaagcca gagtacaaag gcagcctgct gttgaccgac
    28861 gatgcccgtg aagtgttcca gatggcgctg cgtaagctgg gctactccgg taacaccacc
    28921 gatccgaaag agattgaagc tgcatataac gagctgaaaa aactgatgcc aaacgtcgca
    28981 gcgtttaact ccgataaccc ggctaacccg tacatggaag gcgaagttaa cctcggcatg
    29041 atctggaacg gttctgcttt cgttgcacgc caggcgggta cgcctattga cgtggtgtgg
    29101 ccgaaagaag gcggcatatt ctggatggac agcctggcga tcccggcaaa tgccaaaaac
    29161 aaagaaggcg cgctgaaatt gatcaacttc ctgctgcgcc cggatgtggc aaaacaggtt
    29221 gctgaaacta tcggttatcc aacgccaaac cttgcggcgc gtaagctgtt aagtccagaa
    29281 gtggcaaacg ataagacgct ctacccggat gctgaaacca ttaaaaatgg cgaatggcag
    29341 aatgacgttg gcgcagccag cagcatttat gaagagtatt atcagaagct gaaagcagga
    29401 cgttaattca gcaagcgggc ggtggtatta ccgcccgttt tttagttagc aaggatgtgg
    29461 ctaatgagtc aggacagtaa agttttatcc cgctctttcg tcggtattgt ggtccgcttg
    29521 taggcctgat aagcgtagcg catcaggcaa tgcttcctgc tttataatcc ctccagcaac
    29581 ttttcaacaa attctggcac cacctggctt gccgggccgt aatatttctc ggcaaattca
    29641 ttaccaacct gactcggttc aagattcagt tcaacggtgt gagcaccatg tagtttggct
    29701 tcgtgtacaa acccagccgc cggatagaca tgcccggaag taccaatggc aataaagaca
    29761 tccgccatcg acaacgccat ataaatttca tccatgccga gtggcatttc gccaaaccaa
    29821 acgacgtgcg ggcgcaaggg tgccgggaac tggcaacaat ggcatttatc ttctggcgta
    29881 acgtctccgg tccagtcgag aacctgacca ctttgcgaac aacgaacttt cagcaattcc
    29941 ccatgcatat gaatgacatt ggtattacct gcgcgttcat gcaggttgtc tatattctgc
    30001 gttaccagca aaaagcgatc gccgagagcg tcttgcagtt tagccagcgc aagatgcgcg
    30061 gcgttaggct gaatttctgg ctgctgcagc tgtcgacgac gggcgttata aaacgcttgc
    30121 accagttcag gatcgcgatc gaaaccttcc ggcgttgcca catcttcaac ccgatgttct
    30181 tcccacaggc catctgcggc gcgaaaggta cgaatacctg attccgcaga aattcctgcc
    30241 cctgtcagta cgagtactct tggtttttcc attgcttccg gcaccacttt atctctgaaa
    30301 aaaatacgct gacgcaaacg ctcgcgcagg cgacgtttat ttttacgaaa acgacttaac
    30361 cgatgacccc gacgcgacag catagcaacc tctgttgttt aatcggttag atgtaggaag
    30421 gccgcaccac gcattcctcc cgcatcaccg tggcgcgcgc gttcaatgcg cggaacacga
    30481 gctacaggta agagatgaca aggcaacctg tccgccagtt gcgttgtgat tgccgggaaa
    30541 ttcgataagc caccaccaat gacgaccagg tcagggtcaa caatggtcag gatatttccc
    30601 agagaaaccg ctaataaatc cagataacgc tcaacgtgcg cccttgcctg ctcatcgcct
    30661 tgatcataaa gcgcaataat ttcgggagcc tgcaacggtt gatgataata gtgttgatac
    30721 agccacgcaa aaccgcgacc agacagataa ttttcaatgc agccatgctg accacagccg
    30781 cagcggcgta acgggaaatc caaccccatc atggttaacg catcaaccgg cagacgcata
    30841 tggccaaact cgccggtaat gtagctttta ccagtaatcg gtttgccgtt gaaaaccagc
    30901 ccgccgccaa cgccggtgcc gagaattaac cccatcacca gtggatattg cgtaaattca
    30961 tcatcccagg cttctgaaag ggcaaaacag ttggcatcgt tatcaaggcg tacatcgcga
    31021 tcaagacgtg cgctcaggtc ggcacgcagc ggtttgccgc tggcagcggg gacattagcg
    31081 gcatacagct taccatcttc tgtttccggc attcccggaa taccgatgcc gacagaacct
    31141 ttacagccaa aacgttggtc agcttcagct accagctcac acactgcatc taaaaatgcg
    31201 tcatagctgt cacgcggtgt cggcacccgc ttttcccact gcaactgccg accgctatca
    31261 aacacgccaa gcgcaatttt tgttccacca atatcaaacc cgtaatacat tgccgctcct
    31321 tgttttaatg tactgccttt actggccgct aaggactcgc gcagggtcaa tattactggc
    31381 gcgccgcgcc ggataccaac ttgccaaaag actcagcaac aatgctgtga ccagtacgta
    31441 gaagacgtcc agccagtgca attccgatgg caggaagtca ataaaataaa tatcgctgga
    31501 gaggaactga tgaccgatca gcttttcaat ccactcaata atcggggtaa gttgcagtga
    31561 aacaactacg ccgataacca ccccacacag gctgccgaac agccctgcca gcaatccata
    31621 ccagacaaag atggcgcgaa ttaaaccatc tttcgccccc agcgttctta atactgcgat
    31681 atcgccactc ttgtctttca ccgccatcac taaggtggag acgatgttga aacaggccac
    31741 gccaatcacc agtaccatcg ccagatacat aatggcgcgg atcatctgga tatcgcgata
    31801 catatagccg taagtaccaa tccaactttt aatataaaca tagctgttgg tcacttcacc
    31861 cgcatcgcgt accagcttat tggcgttgaa aacatccgtc attttaaggg caatacctga
    31921 cacgctggaa cccatatcaa gatattgttg ggcatccgcc agcgggatca tggcaaaact
    31981 gtgatcgagt tgaccactca actgcaaaat accggcaatg tgcaaacgca cacgttttgg
    32041 ctgcatcagt ttatgctcgg gattcgagtt ggggatcatg atcgacaccc aatcgccctg
    32101 cttcactttc agcgcatccg ccacgccttt gccgatgata atttgctgtt cgcccgcttt
    32161 aaaattgcgc cacgcatccc cctgaacaaa cgagggtaat gcgctcagac gctgttcctg
    32221 ttgtgggtta acgcccttca cctggattgc gcgcagattc gctccacttt ccaccagacc
    32281 ggtgaaattg atatacggcg cagcggcggc aatacccggc actttctgca cgttatccag
    32341 tgcttcctgc cagttagtcc atggctgatt taccgcttcg atttcgccat gcggcaccac
    32401 cgccagaatg cggttattca gttcgcgttc aaagccgttc atcgcgctta agccgacgat
    32461 caataccgcc acgccgaggg caatgccaat ggtagaaatg acagagatta gcgacaccat
    32521 gccgccgcgt cgccgtccgc gactaaaacg caggccaatc aataacgata aaggcatcgc
    32581 cattactccg cccccatcag gctcagttcc gccgtcagac gtccatcacg catttccagt
    32641 tggcggctca tacgtttcgc cagttgcagg tcgtgagtga ccaccaggaa agcggtgccc
    32701 tgcaagcgat tcaactcccc aagcaactgg aagatgctgt cggcgttacg cgcatcgagg
    32761 ttaccggtag gttcatccgc cagtaccaga cgcggattat tgaccagcgc acgggcaatc
    32821 gctacacgct ggcgttcgcc gccagaaagt tcagatgggc ggtgattcgc acgatgctcc
    32881 agccccaccg cttttaacat ctccagtgca cagctgttga tttcagcggg ctttttcttg
    32941 ccaatcagca gtggcatagc cacgttttcc agggcagtaa aatccggcag caggtggtga
    33001 aactgataaa taaagcccag cttctggttg cgcagttctg ctttcgccgc tgaagacagt
    33061 ttgctcatcg gctgaccgtt aaagatcaca tcgccggagg ttggcgtatc cagcccgcct
    33121 aacaggtgca gcaaggtact tttaccggaa ccagagctac cgacaattgc catcatttca
    33181 ccttcgccca cgctgaaact gacattgagc agcacatcgg tttgcacact gccttcctga
    33241 tagcgtttgc acaggttgtc gcattgcaac aggatcttat tcataacgta aagcctcagc
    33301 gggttgtgtg gcggcagcgc gccatgaagg gtaaagcgta gacagcagcg cgatagccat
    33361 cgccaccagc gcaataacaa tgacctgtaa aggttcgata gccaccggca gcgccgcgcc
    33421 atcaagcagg acgccgatta tcggcatcag attatttaac tggctggcaa gcagtgcgcc
    33481 aagcgccgct ccgaggattg caccgataat cccggcgcta gccccttgca ccataaagac
    33541 catcatgatt tgtcgcggag ttaagccttg cgtttgcagg atcgcgactt cgccctgctt
    33601 ctccatcacc atcagcccca gcgaggtaat aatgttaaac gccgcaacag cgacaatcag
    33661 gctcagcagt aagcccatca tatttttttc catgcgtacg gcctggaaca gctcgccttt
    33721 acggtcacgc cagtcctgcc atttgctgcc ttcaggcagt ttttgctgac ttaaagagtc
    33781 aactctcagc ggctcatcca gccacaaacg ccagccggta atattggctg ccggataacg
    33841 catcagacgc gaggcatcct caatattcac cagcatttca tagccatcga cttcactgtt
    33901 ggcggcgaaa gtgccaatca cattgaacag gcgctgactt ggaatacgcc ccatcggcgt
    33961 gaactggctg gcagatggca ccatcacgcg gatttgatca ccgcgattga cgcccaactg
    34021 tgaggcaagt tgttcgccga ggatgacatt atatttcccc ggctcgaggt cagtttgttt
    34081 cacattgacc agatacggcg ttagtggatc tttttgcgcc gggtcgatac cgagcatcac
    34141 cccgaccgcc acgctgcgcg cgctttgcag taccacatca ccggtagtaa taggtgcgac
    34201 gcgattaacg ccgtccagtt tgactgccgt ttccgggagt tgctgcgggt taagagagcc
    34261 atgctcagaa gagagaattg cctgtggcat caggccaagg atgttgtttt gcagctcgcg
    34321 ctcaaagccg ttcatcactg acaatactgt gaccagcgcc atcaccccga gggtaatgcc
    34381 gatggtagaa agccaggaga cgaaacgacc gaagcgatcc actgcacgcc cacgcatgta
    34441 acgcaggcca ataaatagag cgacaggttg gtacatgaaa tccgtctggt tgctgtagca
    34501 aagacacgag tatataagcg aaagcaagat gggtaaacgt ctgaactgta agtgatcgtc
    34561 agggctaatc tgaaaagccc ccgtaaatca atcggctaaa ttgccatatt ttatcatcaa
    34621 tcttgccgcc tgatcttaat ctgaaaaatc atccaataca gacagatacc cattacacct
    34681 cgggtcataa ccagcaggat caaccgcatg taaattaaaa aagagcgtgt tgttctgatg
    34741 aaacaaaaag agctatggat taaccagatc aaagggttat gtatttgtct ggtggtgatt
    34801 tatcactcgg tcattacctt ttatccgcat atgaccactt ttcagcatcc gttatcggaa
    34861 gtcctgagca aatgctggat ctatttcaat ctttaccttg ccccctttcg tatgccggtt
    34921 tttttcttta tatccggcta tttgattcgc cgctatatcg acagcgtacc gtggggaaat
    34981 tgtctcgata aacggatctg gaacatcttc tgggtgctgg cgctttgggg cgtggtgcag
    35041 tggctggcgt taagtgcgct gaaccagtgg ctggcacctg agcgcgattt aagcaatgcc
    35101 tccaatgccg cttatgccga ttccaccggt gagttcctgc acgggatgat caccgccagc
    35161 accagcttgt ggtatctgta tgcgttaatt gtctatttcg tggtatgtaa aatttttagc
    35221 cgtctggcgc tgccactatt cgccttgttt gtactgctga gtgtggcggt taatttcgtt
    35281 cccacgccgt ggtggggaat gaacagtgtg atacgcaatt tgccttatta cagccttggc
    35341 gcatggtttg gcgcaacaat aatgacctgt gttaaagagg tgccgttgcg ccgccatctg
    35401 ctgatggctt ctttgcttac cgttctggcg gtcggtgcct ggttgtttac tatctcgctg
    35461 ctgttgtcgc tggtatcgat tgtggtgatc atgaagctgt tttatcagta cgagcaacga
    35521 ttcggtatgc gctccaccag cctgctgaat gtgattggct ccaacaccat tgctatctac
    35581 accacccatc gcattctggt tgaaatattc agcttaactc tgcttgcgca aatgaacgca
    35641 gcacgctggt cgccgcaagt cgaactgaca ctcctgctgg tttatccctt tgtcagtttg
    35701 ttcatctgta ctgttgcggg cttgctggta agaaaacttt cacagcgtgc attcagcgat
    35761 ctgttgttct ccccgccttc actgcccgct gccgtcagtt actctcgtta aaccatcacc
    35821 ccagccgggg cgattcaatt tgctaaacca tgtcgattac ggataatgaa cagcattgct
    35881 tatcagagat atgcccccat atgttgaggc atatcctaac gagaatctga caaccgttat
    35941 gcctgaacaa tatcgttata cgctgcccgt caaagcgggt gagcagcgtc tgctgggcga
    36001 gttaaccggc gcagcctgtg caacgctggt agcggaaatt gccgaacgtc acgccggtcc
    36061 ggtggtactc attgcaccag atatgcaaaa tgctctgcgt ttgcatgatg aaatcagcca
    36121 gttcaccgat cagatggtga tgaatctggc ggactgggaa actcttccct acgacagttt
    36181 ttcgcctcat caggacatta tctcctcgcg cctttccacc ctttaccagc taccgacgat
    36241 gcagcgtggc atactgattg ttccggtgaa tacgcttatg cagcgcgttt gcccacacag
    36301 ttttctccac ggtcatgcgc tggtgatgaa aaaaggtcag cgcctgtcac gagatgcatt
    36361 acgaacccaa ctggacagcg ccggttatcg ccatgttgac caggtgatgg agcacggcga
    36421 atacgccacg cgcggcgcgt tgctggatct cttcccgatg gggagtgagc tgccttatcg
    36481 tcttgatttc tttgatgatg aaatcgacag cctgcgggtg tttgacgtcg acagccagcg
    36541 cacgctggag gaagtagaag cgatcaatct gctgcccgcg cacgaatttc cgaccgataa
    36601 agcggcaatt gaactgttcc gcagccagtg gcgcgatacc ttcgaagtga agcgcgatcc
    36661 ggaacatatt taccagcaag tgagtaaagg cacattacct gctgggatcg agtactggca
    36721 gccattgttc ttcagcgaac cactgccacc gctgttcagt tatttccctg ccaatacctt
    36781 gctcgtgaat actggcgatc tggaaaccag tgccgaacgt ttccaggctg acacgctggc
    36841 gcgttttgag aatcgcggcg tcgatccgat gcgcccgctg ttgccaccac aatcgctctg
    36901 gctgcgggtg gacgagctct tctcagagct gaaaaactgg ccccgagtgc agctaaaaac
    36961 tgaacattta ccgacaaaag ccgcgaatgc caatttaggt ttccagaaac tgccagacct
    37021 ggccgttcag gcgcaacaaa aagcgccgct ggatgcgctg cgtaagttcc tcgagacttt
    37081 cgacggtccg gtggtgttct cggtagaaag tgaaggtcgc cgtgaagcgc tgggtgaact
    37141 gctcgcacga attaaaattg ctccgcaacg cattatgcgt cttgatgaag ccagcgaccg
    37201 tgggcgttat ctgatgattg gcgctgccga acatggtttt gtcgataagg cgcgtaatct
    37261 ggcgctgatc tgcgaaagcg atctgctcgg tgaacgtgtt gcccgccgtc gtcaggattc
    37321 tcgccgcacc atcaaccccg atacactgat ccgtaacctt gcggagctgc atattggtca
    37381 gccggtggtc catctggagc acggcgtcgg tcgctatgcc ggaatgacca cgctggaagc
    37441 tggcggcatt actggcgagt acctgatgct cacctatgcc aacgacgcca aactgtatgt
    37501 tccggtgtcg tcactgcatc tgattagccg ttacgcaggt ggcgcggaag aaaacgcccc
    37561 gctgcataaa cttggcggcg atgcgtggtc acgcgcgcgg cagaaagcgg cggaaaaagt
    37621 gcgtgatgtg gcggcggaat tgctggatat ctacgcgcaa cgcgccgcca aagagggctt
    37681 cgcgtttaaa cacgatcgtg agcagtatca gttattctgc gacagcttcc cgtttgaaac
    37741 cacgccggat caggcgcagg ccattaatgc ggtacttagc gacatgtgtc agccgctggc
    37801 aatggatcgt ctggtgtgcg gcgatgttgg ctttggtaaa acagaagtgg cgatgcgcgc
    37861 cgctttcctg gcagtagata accacaagca ggtagcggtg ttggtgccta ccacccttct
    37921 tgcgcagcag cattacgaca acttccgcga ccgtttcgcc aactggccgg tacgtatcga
    37981 aatgatctcc cgtttccgca gcgccaaaga gcagacgcaa atccttgcgg aagtggcgga
    38041 agggaaaatc gatattctga tcggtacgca caaactgctg caaagtgacg tcaagtttaa
    38101 agatttaggc ctgctgattg tcgatgaaga acaccgcttc ggggtgcgtc ataaagagcg
    38161 cattaaagcg atgcgcgcga acgtggatat tctgacgctt actgcaacgc cgatcccacg
    38221 tacgctgaat atggcaatga gcggaatgcg tgacctgtcg attatcgcca cgccgcccgc
    38281 ccgtcgtctg gcagttaaaa cctttgtccg tgagtatgac agcctggtgg tccgggaggc
    38341 gatcctgcgt gaaattttgc gcggaggaca ggtttattat ctctacaatg atgtggaaaa
    38401 cattcagaaa gccgccgaac ggctggcaga actggtgcct gaagcacgga ttgccatcgg
    38461 tcacgggcag atgcgcgagc gcgaactgga acgggtgatg aatgatttcc atcatcaacg
    38521 tttcaacgtg ctggtttgta ccaccattat cgaaaccggg atcgacatcc cgacagccaa
    38581 caccattatc attgaacgtg cggatcactt cggtctggcg cagctgcacc agttacgcgg
    38641 tcgcgtcgga cgttcacatc atcaggcata tgcatggctg ctgacgccgc atccaaaagc
    38701 gatgactacc gatgcacaaa aacgtcttga agcgattgcc tcgctggaag atctcggggc
    38761 aggttttgcg ctggcaacgc acgatctgga gattcgcggc gcgggtgaac tgcttggcga
    38821 agaacaaagc ggctcaatgg aaaccatcgg tttctcgctg tatatggagt tgctggaaaa
    38881 cgccgtcgat gcactgaaag ccggacgcga gccgtcgctg gaagatctca ccagccagca
    38941 aacagaagtc gagctgcgga tgccgtcgct attgccagat gatttcatcc ctgacgtgaa
    39001 cacgcgtctg tcgttctaca aacgtattgc cagcgccaaa acggaaaacg aactggaaga
    39061 gatcaaagtc gagcttatcg atcgcttcgg cctgctgcca gatccggcgc gtaccctgct
    39121 ggatattgcc cgtctgcgcc agcaagcgca gaaactgggg atcaggaagc tggaaggtaa
    39181 tgagaaaggc ggggtgatcg aatttgccga gaagaatcac gttaatccgg cctggttgat
    39241 tggtttgctg caaaaacagc cgcagcatta ccgccttgat ggtccgacgc gcctgaaatt
    39301 tattcaggat ttgagtgagc ggaaaacgcg tatcgaatgg gtacgccagt ttatgcgtga
    39361 actggaagag aacgcgatcg cttaatgtaa atcgggggcg tttctgcgcc cccatctgca
    39421 acatttacaa attctttgca ctttcctgca ctatccgaca ctgtcaccat ccataattca
    39481 ggcttatctg tttattacaa taaccttata tttattatgt atttttggtg atgatcaaaa
    39541 cgcgtttttc tcgctggcta acgtttttta cgttcgccgc tgccgtggcg ctggcgctac
    39601 cggcaaaagc caacacctgg ccgctgccgc cagcgagcag tcgtctggtt ggcgaaaaca
    39661 aatttcatgt ggtggaaaat gacggtggtt ctctggaagc catcgccaaa aaatataacg
    39721 tcggctttct cgctctgtta caagctaacc ccggcgttga tccttacgta ccgcgcgcgg
    39781 gcagcgtgtt aacgatcccg ttgcaaaccc tacttccaga cgcgccgcgc gaaggcattg
    39841 tgatcaacat tgcggagctg cgtctctatt actacccgcc gggtaaaaat tcggtaaccg
    39901 tgtatccaat aggtattggt cagttaggtg gtgacacgct gacaccgaca atggtgacca
    39961 ccgtttcaga caaacgtgca aacccaacct ggacgccaac ggcaaacatc cgcgcccgtt
    40021 ataaagcaca gggaattgag ttgcctgcgg tagtgccggc tggaccggat aacccaatgg
    40081 gccatcatgc gattcgtctg gcggcctatg gcggcgttta tttgcttcac ggtacgaacg
    40141 ccgatttcgg cattggcatg cgggtaagtt ctggctgtat tcgtctgcgg gatgacgata
    40201 tcaaaacact ctttagccag gtcaccccag gcaccaaagt gaatatcatc aacactccga
    40261 taaaagtctc tgccgaacca aacggtgcgc gtctggttga agtacatcag ccgctgtcag
    40321 agaagattga tgacgatccg cagctgctgc caattacgct gaatagcgca atgcaatcat
    40381 ttaaagatgc agcacaaact gacgctgaag tgatgcaaca tgtgatggat gtccgttccg
    40441 ggatgccggt ggatgtccgc cgtcatcaag tgagcccaca aactctgtaa atcctggata
    40501 aaaaaaatcc ccgcgacacg gcatgtcgcg gggaaaaaat cagcagtagc aggcattaat
    40561 gagggttaat gcttatttat aaattactgc tgttccatgg agggtattcg gaccggttac
    40621 agaagtaata cggaaagatt ttgcgcccat ctcatccgct ttttgcgcca gctgctcttc
    40681 cagcgatccc agatttgtcc ccgcgttagc actgatagta ccgacttttt gttggccttc
    40741 tggcgttgac tgaacttcga cagccgcaaa gttggcaaat gacatggagc ttaaaatcgc
    40801 cgcagcgatg aggtttttac gtttttcata atagtggcct tatgcagatg aatgacgtcg
    40861 caagaaatta cttaacgatc gataactaaa tgatagatgt gatctggatc acatacaaga
    40921 tattttttat aacaatcatt aattaattta aaaatc
//
LOCUS       DAZSNO010000020        40846 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZSNO010000020 DAZSNO010000000
VERSION     DAZSNO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40846
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.020"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..155
                     /locus_tag="ACHWN2_001026"
     CDS             <1..155
                     /locus_tag="ACHWN2_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8930659.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="ACHWN2_001027"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="ACHWN2_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR8930660.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="ACHWN2_001028"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="ACHWN2_001028"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR8930661.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLSIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="ACHWN2_001029"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="ACHWN2_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="ACHWN2_001030"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="ACHWN2_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR8930662.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="ACHWN2_001031"
     CDS             complement(5626..7134)
                     /locus_tag="ACHWN2_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR8930663.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHWN2_001032"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHWN2_001032"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR8930664.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="ACHWN2_001033"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="ACHWN2_001033"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR8930665.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="ACHWN2_001034"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="ACHWN2_001034"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR8930666.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHWN2_001035"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHWN2_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR8930667.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHWN2_001036"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHWN2_001036"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR8930668.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="ACHWN2_001037"
     CDS             complement(14283..15602)
                     /locus_tag="ACHWN2_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFR8930669.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHWN2_001038"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHWN2_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR8930670.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="ACHWN2_001039"
     CDS             16699..17034
                     /locus_tag="ACHWN2_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR8930671.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHWN2_001040"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHWN2_001040"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR8930672.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="ACHWN2_001041"
     CDS             complement(17790..19172)
                     /locus_tag="ACHWN2_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8930673.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHWN2_001042"
     CDS             complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHWN2_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR8930674.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="ACHWN2_001043"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="ACHWN2_001043"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR8930675.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="ACHWN2_001044"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="ACHWN2_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR8930676.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHWN2_001045"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHWN2_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR8930677.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHWN2_001046"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHWN2_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR8930678.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHWN2_001047"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHWN2_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR8930679.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="ACHWN2_001048"
     CDS             complement(25947..26768)
                     /locus_tag="ACHWN2_001048"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR8930680.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="ACHWN2_001049"
     CDS             complement(26868..26951)
                     /locus_tag="ACHWN2_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930681.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHWN2_001050"
     CDS             complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHWN2_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR8930682.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            27623..27727
                     /locus_tag="ACHWN2_001051"
     CDS             27623..27727
                     /locus_tag="ACHWN2_001051"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR8930683.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(27776..29029)
                     /locus_tag="ACHWN2_001052"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="ACHWN2_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30056
                     /locus_tag="ACHWN2_001053"
     CDS             29136..30056
                     /locus_tag="ACHWN2_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR8930684.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAELAKEPFVFFDPHVGTGLYDDI
                     LGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDA
                     VSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30191..31411
                     /gene="mlc"
                     /locus_tag="ACHWN2_001054"
                     /gene_synonym="dgsA"
     CDS             30191..31411
                     /gene="mlc"
                     /locus_tag="ACHWN2_001054"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR8930685.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31536..32231
                     /gene="bioD"
                     /locus_tag="ACHWN2_001055"
     CDS             31536..32231
                     /gene="bioD"
                     /locus_tag="ACHWN2_001055"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR8930686.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32184..33476)
                     /gene="clcB"
                     /locus_tag="ACHWN2_001056"
     CDS             complement(32184..33476)
                     /gene="clcB"
                     /locus_tag="ACHWN2_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR8930687.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISQTLHRDSIYRQHTAQHS"
     gene            complement(33635..34249)
                     /gene="dmsD"
                     /locus_tag="ACHWN2_001057"
     CDS             complement(33635..34249)
                     /gene="dmsD"
                     /locus_tag="ACHWN2_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR8930688.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34292..35146)
                     /gene="ynfH"
                     /locus_tag="ACHWN2_001058"
     CDS             complement(34292..35146)
                     /gene="ynfH"
                     /locus_tag="ACHWN2_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR8930689.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35148..35765)
                     /gene="ynfG"
                     /locus_tag="ACHWN2_001059"
     CDS             complement(35148..35765)
                     /gene="ynfG"
                     /locus_tag="ACHWN2_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR8930690.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35776..37814)
                     /locus_tag="ACHWN2_001060"
                     /pseudo
     CDS             complement(35776..37814)
                     /locus_tag="ACHWN2_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(37875..38909)
                     /locus_tag="ACHWN2_001061"
                     /pseudo
     CDS             complement(37875..38909)
                     /locus_tag="ACHWN2_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            38967..40556
                     /locus_tag="ACHWN2_001062"
     CDS             38967..40556
                     /locus_tag="ACHWN2_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR8930691.1"
                     /translation="MTDINHDNTAYPSSYFSGSPYLYGQVYTPSPDTRLTQINLLGVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNHTDYSTIGDSTSILKNEQGDRPVVFTQE
                     RQPEVTICSFSELVSSLLAEKVQTLKSSSYKDLSSSLNTVSRFLPEDMDLMSRSEWLA
                     VRDAMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDVDSKRRAFT
                     DEEMERLLVRVESEYQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGACGLDLKSFLEWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDATDVSLHSLRHWLATRMKERGANLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLAEVLKTALL"
BASE COUNT        10359 a         9903 c        10478 g        10106 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaagcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagagt acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgtgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gctgaactgg cgaaagaacc ctttgtcttt tttgatccgc
    29761 acgtcgggac tgggctgtat gacgatattc tcggactgat gcgacgttac catttgacgc
    29821 ccgtcatcac tcaggaggtg ggcgaggcaa tgaccatcat cggtctggtt tccgccggtc
    29881 tgggtgtttc aattttgcct gcgtcattta aacgtgttca gctcaacgaa atgcgctggg
    29941 tgccgattgc tgaagaggat gcggtttctg aaatgtggtt ggtctggccg aaacatcatg
    30001 aacaaagtcc agctgcgcgt aactttcgta ttcatctgct gaatgctctc aggtgaggga
    30061 aatttcagcg aaaaagcccg aaaaatgtgc tgttaatcac atgcctaagt aaaaatttga
    30121 cgacacgtat tgaagtgctt caccatagcc tacagattat ttcggagcgc gaaaatatag
    30181 ggagtatgcg gtggttgctg aaaaccagcc tgggcacatt gatcaaataa agcagaccaa
    30241 cgcgggcgcg gtttatcgcc tgattgatca gcttggtcca gtctcgcgta tcgatctttc
    30301 ccgtctggcg cagctggctc ctgccagtat cactaaaatt gtccgtgaga tgctcgaagc
    30361 acacctggtg cgagagctgg aaatcaaaga agcggggaac cgtggccgtc cggcggtggg
    30421 gctggtggtt gaaactgaag cctggcacta tctttctctg cgcattagtc gcggggagat
    30481 tttccttgct ctgcgcgatc tgagcagcaa actggtggtg gaagagtcgc aggaactggc
    30541 gttaaaagat gacttgccat tgctggatcg tatcatttcc catatcgatc agttttttat
    30601 ccgccaccag aaaaaacttg agcgtctaac ttcgattgcc ataaccctgc cgggaattat
    30661 tgatacggaa aatggtattg tacatcgcat gccgttctac gaggatgtaa aagagatgcc
    30721 gctcggcgag gcgctggagc agcataccgg cgtaccggtt tatattcagc atgatatcag
    30781 cgcatggacg atggcagagg ccttgtttgg tgcctcacgc ggggcgcgcg atgtgattca
    30841 ggtggttatc gatcacaacg tgggggtggg cgtcattacc gatggtcatc tgctacacgc
    30901 aggcagcagt agtctcgtgg aaataggcca cacacaggtc gacccatatg gtaaacgctg
    30961 ttattgcggg aaccacggct gcctcgaaac catcgccagt gtggacagta ttcttgagct
    31021 ggcacagctg cgtctcaatc aatccatgag ctcgatgtta catgggcaac cgttaaccgt
    31081 ggactcattg tgtcaggcgg cattgcgcgg cgatctactg gcaaaagaca tcattatcgg
    31141 ggtgggcgcg catgtagggc gcattcttgc catcatggtg aatttattta acccacaaaa
    31201 aatactgatt ggctcaccgt taagtaaagc ggcagatatc ctcttcccgg tcatctcaga
    31261 cagcatccgt cagcaggccc ttcctgcgta tagtcagcac attagcgttg agagtactca
    31321 gttttctaac cagggcacga tggcaggcgc tgcactggta aaagacgcga tgtataacgg
    31381 ttctttgttg attcgtctgt tgcagggtta acatttttta actgttctac caaaatttgc
    31441 gctatctcaa tttgggccag gaaagcataa cttagacttt caaggttaat tattttccta
    31501 gtttatattt gtgaagcata acggtggagt tagtgatgct gaagcgtttc tttattaccg
    31561 gtacagacac ttctgtaggg aaaacggtgg tttcccgcgc attgctacaa gcgttagcct
    31621 cccagggaaa aagggttgcg ggatataaac ccgtagcgaa ggggagcaaa gagacacccg
    31681 aagggctgcg taataaagat gccttggtgt tgcagagtgt ttcaaccatc gaactgcctt
    31741 atgaagcagt taatcctatc gcgttaagcg aagaagaaag tagcgtggcg cacagttgcc
    31801 caatcaatta caccctcatt tcaaacggcc tggcaaacct gaccgataaa gtcgatcatg
    31861 tcgtggtaga agggactggc ggctggcgca gtctgatgaa tgatttgcgt ccactctctg
    31921 aatgggtagt gcaggaacaa ctgccggtgt tgatggttgt cggtattcag gaaggttgca
    31981 ttaaccatgc actgctaaca gctcaggcga tcgccaacga cgggctgccg ctcattggct
    32041 gggtggctaa ccgaatcaac ccaggactgg cgcattatgc ggaaatcatt gatgtgctgg
    32101 gtaaaaaact tccggcaccg ctcattggtg aactgcctta tctgccgcgc gccgaacagc
    32161 gtgaactggg gcaatacatc cgcttagcta tgctgcgcag tgtgctggcg gtagatagag
    32221 tcacggtgta acgtctgcga aattaccgac gcaattacgc aggcaatcaa taaaccgggg
    32281 agtagctgat actccccggt catttcacat atcatcaacg tcgacataat cggcgcgtgc
    32341 gtggttgccg ccaacagtgt cgccatcccg gtcaatccga gtaaaagcgt aatttcttcg
    32401 ccatcaggga accataatcc caggctacga ccatacaaca tgccaatggc aagaccgata
    32461 aatagcgtcg gtgtaaagac accaccgggt gcgccggaac cgctactcgc cagcacggca
    32521 cacagtttac aaaggaagat cccggcaatg atcattaaca gtggtggggc ggttaaaaag
    32581 gattgtacgg tgctatagcc gttgccccac actgcaggtg tgaacaggga aagcagaccc
    32641 acgatcaacc cgcctagtgc cagttgccag ggcggcgcaa gtttgagact cacaaatcca
    32701 cgatgacagg cgttcattaa cgttaacaac agtggtccgc acagacctgc cagcacacct
    32761 gtactgataa tcagcgcata gtcacgagcc tgaaccgtca ctgagagttg tacgttgtag
    32821 agtaacgcgt cgctatgatt aatcagattg ctaaccagcc atgccacgat ggcggaaata
    32881 atcaccgggc cgagagaggc caacatcata gtgccaaaca gcacttcggc tataaataaa
    32941 ctgccagcaa gcggggcacg ataggccgca gccattcccg ccgcggcccc acaggcgatc
    33001 cataatttcc attcctggcg tggcgtaaaa cgttgggcaa aacaggaggc ggcaagggca
    33061 gctaaaagaa tcatcgcacc ttcgcgacca attgcactgc cgctggttac taccagcagc
    33121 gaggcaagcg atttaaccag gcttgctgcg taatcgaact gtccatcggt ttgcaacgct
    33181 tccatgtaat cggtcggcgc atgagggcgt tgttgggtaa atttctgcca gcccatcagc
    33241 aacaaacccg ccgccagtcc gccgagcgcc ggagttagca gccgtcgcca ggaggaaagg
    33301 tttgtcgctg cattgaccag actgccggag tcattattga ggaacaacca ctccagtagc
    33361 agcatcgcat gacgaaaccc ggcaacggca aagacagcga gaataccgac gactgttgcg
    33421 ataagcagac ggcggaacat agtgcgcaga tccggataga tgtgtagatg atgcatgagc
    33481 agaaatgggc aatgaaacac gggcgactat tttgcggcaa aagaggccaa ccgcaaaagc
    33541 agtgcgtgct ttaagagata aaaaaacgtg acattgtaac gtattattgc cgaatcgcgc
    33601 gtaatcgtca ccatccggca atattacggt gatcctatcg aaagagtggt ttaaccgcga
    33661 caggaatcaa cagttgtgat tgccattgcg ccagcgttaa gcgagccagt tcacccagtg
    33721 cacggtagaa agggtgttct gctttttcga taaaaacatc aagaaaacgt gttgaccacg
    33781 gaaaaaggtg ccatgccagc aattcttcgc attctgtctg gcgaccattc tccgccaacc
    33841 acgcagccat caacagcagt gaaccaaaat gatcttccgg ttcgttttgc ttcatttcaa
    33901 actgaatgcc tttctcgcgc atccactgac gaagtgccaa tgttgaatcg ccaaacagca
    33961 cagattcgcg atccagccag accgaccccc acggcggaga cggcagtgcc cacgggccga
    34021 caaacaaacg ctgccaggcc tgggcgtgag tctcttcaca ctgtgtctga aaagccgtca
    34081 ccagcggcgc taatgacgct tctggtagag gccactgagt ttcccagccg tcactggtga
    34141 gtaccgcaac cagaggtgcg gcttccgcac tctctggagc gtaataaaac agcgcgccca
    34201 acacgcgcgc cgcgacagaa aaattatctt gctgtgaaaa atgggtcatt cctaacatcc
    34261 tgaaatgtgc gggttgcccc gcaccaattt gttaacctgc aatagccatt cctactgtca
    34321 tgtgcaggcc ataaaagagc acacgcccga tcatctcacc aacaagtatg aggattagcc
    34381 ccagaacaag tccggcaacg tgaggttcac ggcgacgaat gagcggacaa agccagcatc
    34441 caagacctgc gcataacaat accacacgcc agacctgtaa ggaggcgtaa tctggtacca
    34501 gtgcgctggc ttgttgcacg gatgagtgaa tagacgccag agacaaacct tgcagcacaa
    34561 tcacctcggc acaagcgatc agcgccagaa cgctaatgat ggcaaatggt gtggtattaa
    34621 atgtaacgcg ggctgcccgc agaattgcgg cagcaagtat tggaccgctc aacaataccg
    34681 tcaggaagaa agccagcgta gtgtaaccgt tatgccaggt tggcacggtg tcgatttgat
    34741 acacgcaggt catcatccag acgaaaatga cgccgagcgc catgctgaca agtaaccaga
    34801 gtttccccaa cgcttgcggc attttaccga tgaccgccac cagccaccat agtccgccaa
    34861 cggcaaaaaa tatggaaccg gcagcaatct cgttactcag accagaagct ccgattcgat
    34921 tcagcgagtt aaacgctcgc agtggcgatc ccaggtgcat gacagaggca ataaacccga
    34981 tgcccatcaa cagccagagg aaaaacatgc cacgaacaat gtgctgacgg tctgcgtcat
    35041 ttttggcggc gaaccagcca attccgctaa caatcagcgc gcctactacg cattgaccca
    35101 atacggtaaa gataaccagt ggccattcat gccatccatt tcccatttta cacttcctcc
    35161 ggattagcca gataaccagt ggtatcgccg gtcgggcggc tgttggcgtt aggtttgata
    35221 acgatattgg gttttgtgaa atgcgcgcgc ggcagtggcg cgacggcagc cagagtgccg
    35281 tgcttctggc gcagctcttc aattggaccg aactcaagtg cacgcagcgg gcaggattcg
    35341 acgcatatgg gttgtttccc ctcggcgacg cgcgaataac aaccatcgca cttcgtcatg
    35401 tgcccttttt cagcattgta ctgtggtgcg ccgtacggac aagccatgtg gcagtagcgg
    35461 cagccgatac aaacatcttc atcaaccacc acaaagccat cttcacgctt atgcattgcg
    35521 ccgctggggc agacttttgt acatgcgggg tcatcgcaat ggttacagga gatggagaga
    35581 taataagcga acacgttctg gtgccagatg ccattatctt cctgccagtc gccgccagca
    35641 tattcataaa tacggcggaa actgacttcc gggccaagat ctttaaaatc tttgcacgcc
    35701 agttcgcagg ttttacagcc agtacagcgg ctggaatcga taaaaaatcc atattgtgtg
    35761 gtcatgggct actccttaaa ccttttcgat ctggacaaga ttgctgtgcg acgggtttcc
    35821 ctttgccagc ggtgaagggc ggtgagaggt cagaatattg atactgccgc cgtgatcgac
    35881 ccggtcacca aacatatccg ctttaagcca cgcaccttgc ccgatggcgg taacgccagg
    35941 cagaatacgc ggagtcactt ttgcggcaat cagcatttct ccattattgt taaatacccg
    36001 cacggtatcg ccatgacgga taccgcgtgc ctgagcatca atggggttga tccacacctc
    36061 ttgtgggcag gcctgctgta acacatcaat attgccgtag ctggagtggg tacgcgcttt
    36121 ggtttggcga cttcactcag catccagtag atgggtttgc gttcaaattt tgctgaggtt
    36181 gcgggttggg cgaggatcac atagcccata ttccctgcag attcatgaga gataaggtct
    36241 tcttgctctg ttggcatcag gtcaggcaac aggatatcgc aatacttagc cgaggccgtc
    36301 atgaagtggt caatgccaac aatcatctcg cacttgctgt catcctgaag cacctcatgg
    36361 gtgtgattga tgtcgccatg ttgattgatc aatgtgttac tggcgtagca ccataaaaac
    36421 ttgatgggga catccagttt ttcttttcca cgaacaccat cacgggtcgc ggtcattttc
    36481 gtaccatggt cgatggcatc tgtccatgta aaaacggaaa tctgcgtttt aacaggattc
    36541 tcgagcatcg ggaaccattc tacccccaga tcccagctac cttcgcgtac acctgagttg
    36601 ccgccgttta tgccgacgtt accggtaaga acggaaagca tggcaatagc gcgggacgtt
    36661 tgttcgccgt tggagtgtcg ttgtggcccc caaccctgac aaatataagc aggttttgct
    36721 gaaccgatct ctcgtgccaa ctggataatt ttttctgccg ggatgctggt gatttttgct
    36781 gcccattccg gcgttttagc tatgccgtca ggcccttcgc ccagaatata ggctttataa
    36841 tgcgcgttac gtggtgcgtt ggcgggcagc gttttttcat cgtaaccaac acaatatttg
    36901 tcgagaaatg gctgatcgac catgttttca gtaatcagta cccaggcaat cgcacaggcc
    36961 agtgcgccat cggtgccagg gcgaatgggc agccattcat cttcacgccc ggcagctgtg
    37021 tcgttatagc gtggatcgat gacgatcatg cgtgcgtttg aacgttcgcg ggcttgctcg
    37081 acgtagtaag tgacaccacc gccgctcatc cgcgtttctg ccgggttatt tccgaacata
    37141 acgaccagtt tcgtattggc gatatcatcc gggctgttgc catcattggc accgaacata
    37201 taactcattg cggcactgat ctgtgcggta ctgtagctgc catagcgact gagaaaaccc
    37261 ccgcaagagt tcatcagacg gtacgggacg ttcgagttgg tgatatttcc gccatctacg
    37321 cctgttccgt acaggacatg tacagcctca ttgccgtaat ctttcaggat ccgccgaaga
    37381 ttatcactga tggtatccag ggcttcgtcc caacttatcc gttcaaattt accttcaccg
    37441 cgctcgccga cgcgcttcat gggatatttc aacctgtcag gatgattcat ccgtcggcgg
    37501 atagagcgcc cgcgtaaaca tgcccgaacc tgatgattac cgtaaacgtc gtcacctgtc
    37561 gtatcagact ccacccagta cacggtgtca tctttcacat gcaaacgtag cagacagcgg
    37621 ctcccgcagt taacggtgca ggaactccag accgttttct cttctaccgg agcctctgcc
    37681 gcccggacca tttgggaaaa tggcagagtg aaagcactgc ttgccagcgc gagactgcca
    37741 agtgcggagg ttttcatcag acttctatgg ctgatttcag ccttcatgag cgcctctgtg
    37801 gtatggattt tcatcattac tcacttattg cttttcaaac aaaatgtcat gccagaattt
    37861 atggttgtag tgggttatat tttttcgatc tcgaccagat tagtgtgctg cgggtttccc
    37921 ttcgccagtg gtgaagggcg cagagtggtt agcgtattca cacatccgcc atggtcgatt
    37981 ttatcgccag acatattggg ctcgtgccag gctccctggc ccatagcgct aactccaggg
    38041 agaatgcgtg gtgttacttt ggctggtagc cgaacttcgc cacgatggtt aaacacccgc
    38101 accatatcgc cgttggcaat cccacgtttc tgtgcatcta tagggttgat ccacacctcc
    38161 tgacggcagg ctgccttcag gacatcaatg ttgccgtagg tcgagtgagt acgggatttg
    38221 taatggaaac caaacagttg cagtgggaag gttctacgtt caggggagtc ccagccttca
    38281 aaggttgagg cataaactgg cagtgggctt atcacttcat ctttttccag ttcccaggta
    38341 cgggcaattt ccgccagctt gctggaataa atttcaatct taccggaagg cgttttaagt
    38401 ggatttgcct cggggtcgtc acgaaatgct ttgtaggcga caaaatggcc attgggatct
    38461 ttacgcttat agatacccat ttttttcagt tcgtcgtaag acggtaacgc cggatctttg
    38521 gcaagcattt tggcgtacag atgttgtaac cattgttcct gcgtgcgacc ttctgtgaac
    38581 ttttgataga cgtcaggtcc aagacgtttc gcgacttcac tcaggatcca gtaaatcggt
    38641 ttacgttcga atttttcgct ggtgactggc tggaggaaaa tgagatatcc catgttaccg
    38701 gcgtagtcgt taggaataat atcttcctgc tcaacggtca tcaggtcttc cagcagaatg
    38761 tcggcatatt ttgccgatga ggtcataaag ttttcgatga ccacaatcat ttcgcatttc
    38821 gattcgtcct gcagaatttc atgcgttttg ttgatgtcag aatgctgatt aacgagggta
    38881 tttcccgcgt agttccagat ggactccact gtcagacgga tggacggact tgaagtagtt
    38941 gactcaacgc tacttaaggt cctgatatga ctgacattaa ccatgacaac actgcatatc
    39001 catccagtta tttctctggg tctccctatc tgtatgggca agtctacacc ccaagtcctg
    39061 acacaaggct gacacaaatc aatctactcg gcgtagctaa gcctcagtta gtccgtagag
    39121 ccaatggtcg atatactatc cgcttcagac tcaagggaca gaccacaccg tttctttcag
    39181 tatcgacccg cagcacagac aggagagtag ccacaatgcg tcagagagag cttgcagcca
    39241 cagctaaggc cttcatgttg gatagacctg aagtctcact acaggagctt acagagcatc
    39301 tccggtctat ggctgagcag ttcctgacag acgctagtga cgattactgg aatggacttg
    39361 aggtcgctac tctggtcgac gagaagtcca acctgaagga gctagccgct acgcaagcct
    39421 tgagtctgga ccagcagaag ggcataaggt tagcgctgga agtcctgacc gcagcacagc
    39481 agagagtcga cactggtgat acctctggtc tgattaagct gattgatgat aataaccata
    39541 ctgattactc aactataggc gactctacgt caattctgaa gaatgagcag ggggatagac
    39601 cagtagtttt cacacaggag cgccaacctg aggtcactat ctgtagtttt tctgagctag
    39661 tgtcctcgct gctggctgag aaggtccaga ccctgaagtc ctccagctac aaggacctct
    39721 cgtcctcact aaatactgtc tcccggttcc tgcctgaaga catggacctg atgtctcgct
    39781 ctgagtggct ggctgtcagg gacgctatgc tggcttctga ggtgagaccg tcgacgatta
    39841 acaagctgct gactaaggcg aagatgtgtc tggactatgg cctgatgaat gggcaacttg
    39901 agggccgcaa cccgattgag cgcatgaagt taactaagga tgtagactcc aagcgtagag
    39961 cattcactga cgaggagatg gagagacttc tggtccgtgt tgagtctgag tatcagttca
    40021 cacgacacac ggctcacacg acctctgagg cccgtagatg ggcgtctctg gtgtctgtag
    40081 ttactggcgc tcggtctgct gaggtctgcc acctgactaa acgtgacatt gtaaccttgg
    40141 acaatgggct ggtctgtatc gacatcaatg aagatgggga cggtaagtct gtgaagaaca
    40201 agcattcagt ccgacttgtg ccgttgaccg atggggcctg tggtcttgac ctgaagtcct
    40261 tcctcgaatg ggtagacatg cagcctgacg agggtcctct cttcgggatg actccaagcg
    40321 catactctag ctggtttaac tcaagggtcc tgactgaagc tctgggagac gctacggatg
    40381 tctctctcca cagtctgagg cactggctag cgaccagaat gaaagagcgt ggagccaatc
    40441 tggtggacgc tcagggcatc ttaggtcaca gcagtcagtc cattacgtat gacctgtacg
    40501 ggaaaggaca cgctgttggt aggctggctg aggtgctgaa gacagcttta ctttaagtag
    40561 gaagacccaa ggtgggagac catatacggc ttgatggttg agaccttggg tcactgaagt
    40621 ctgactgaag ttagggaggg tgataataat cataatccct cctgactata ggcccactgg
    40681 ttcgccttcg gctgtccgac tctaagtctg tctgtggtct tatttaaact ggttccctaa
    40741 ctcaggtcct ccaacttggt gtcttactta aactggttcc ctaactcagg cccctccaaa
    40801 tttcgttgtc tgtcagcgac ttaaatatcc cctcacctta gaagag
//
LOCUS       DAZSNO010000021        40543 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZSNO010000021 DAZSNO010000000
VERSION     DAZSNO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40543
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.021"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..956)
                     /locus_tag="ACHWN2_001063"
     CDS             complement(<1..956)
                     /locus_tag="ACHWN2_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFR8930692.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="ACHWN2_001064"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="ACHWN2_001064"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFR8930693.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHWN2_001065"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHWN2_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHWN2_001066"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHWN2_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR8930694.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="ACHWN2_001067"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="ACHWN2_001067"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR8930695.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="ACHWN2_001068"
     CDS             complement(5018..6346)
                     /locus_tag="ACHWN2_001068"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR8930696.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="ACHWN2_001069"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="ACHWN2_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="ACHWN2_001070"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="ACHWN2_001070"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR8930697.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="ACHWN2_001071"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="ACHWN2_001071"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR8930698.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="ACHWN2_001072"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="ACHWN2_001072"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR8930699.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="ACHWN2_001073"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="ACHWN2_001073"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR8930700.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="ACHWN2_001074"
     CDS             complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="ACHWN2_001074"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR8930701.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15311..16546
                     /gene="moeA"
                     /locus_tag="ACHWN2_001075"
     CDS             15311..16546
                     /gene="moeA"
                     /locus_tag="ACHWN2_001075"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR8930702.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16546..17295
                     /gene="moeB"
                     /locus_tag="ACHWN2_001076"
     CDS             16546..17295
                     /gene="moeB"
                     /locus_tag="ACHWN2_001076"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR8930703.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="ACHWN2_001077"
     CDS             complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="ACHWN2_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR8930704.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18267..19166
                     /locus_tag="ACHWN2_001078"
     CDS             18267..19166
                     /locus_tag="ACHWN2_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR8930705.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19172..21604
                     /locus_tag="ACHWN2_001079"
     CDS             19172..21604
                     /locus_tag="ACHWN2_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR8930706.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21750..22565
                     /gene="ybiV"
                     /locus_tag="ACHWN2_001080"
     CDS             21750..22565
                     /gene="ybiV"
                     /locus_tag="ACHWN2_001080"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR8930707.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22717..23982
                     /locus_tag="ACHWN2_001081"
     CDS             22717..23982
                     /locus_tag="ACHWN2_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR8930708.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRLLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24223..25815)
                     /locus_tag="ACHWN2_001082"
     CDS             complement(24223..25815)
                     /locus_tag="ACHWN2_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR8930709.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26034..26954
                     /gene="ldtB"
                     /locus_tag="ACHWN2_001083"
     CDS             26034..26954
                     /gene="ldtB"
                     /locus_tag="ACHWN2_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR8930710.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27012..28129)
                     /locus_tag="ACHWN2_001084"
                     /pseudo
     CDS             complement(27012..28129)
                     /locus_tag="ACHWN2_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
     gene            complement(28126..28593)
                     /gene="mntR"
                     /locus_tag="ACHWN2_001085"
     CDS             complement(28126..28593)
                     /gene="mntR"
                     /locus_tag="ACHWN2_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR8930711.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28779..28907
                     /gene="mntS"
                     /locus_tag="ACHWN2_001086"
     CDS             28779..28907
                     /gene="mntS"
                     /locus_tag="ACHWN2_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR8930712.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29179..30762
                     /gene="opgE"
                     /locus_tag="ACHWN2_001087"
     CDS             29179..30762
                     /gene="opgE"
                     /locus_tag="ACHWN2_001087"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR8930713.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30810..31325)
                     /gene="ompX"
                     /locus_tag="ACHWN2_001088"
     CDS             complement(30810..31325)
                     /gene="ompX"
                     /locus_tag="ACHWN2_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR8930714.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31378..31443)
                     /locus_tag="ACHWN2_001089"
     CDS             complement(31378..31443)
                     /locus_tag="ACHWN2_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930715.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31678..32565
                     /gene="rhtA"
                     /locus_tag="ACHWN2_001090"
     CDS             31678..32565
                     /gene="rhtA"
                     /locus_tag="ACHWN2_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR8930716.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32864..33367
                     /gene="dps"
                     /locus_tag="ACHWN2_001091"
                     /gene_synonym="pexB"
     CDS             32864..33367
                     /gene="dps"
                     /locus_tag="ACHWN2_001091"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR8930717.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33771..34517
                     /gene="glnH"
                     /locus_tag="ACHWN2_001092"
     CDS             33771..34517
                     /gene="glnH"
                     /locus_tag="ACHWN2_001092"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR8930718.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34656..35315
                     /gene="glnP"
                     /locus_tag="ACHWN2_001093"
     CDS             34656..35315
                     /gene="glnP"
                     /locus_tag="ACHWN2_001093"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR8930719.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35312..36034
                     /gene="glnQ"
                     /locus_tag="ACHWN2_001094"
     CDS             35312..36034
                     /gene="glnQ"
                     /locus_tag="ACHWN2_001094"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR8930720.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36295..38519
                     /gene="ybiO"
                     /locus_tag="ACHWN2_001095"
                     /pseudo
     CDS             36295..38519
                     /gene="ybiO"
                     /locus_tag="ACHWN2_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38516..39442)
                     /gene="rlmF"
                     /locus_tag="ACHWN2_001096"
     CDS             complement(38516..39442)
                     /gene="rlmF"
                     /locus_tag="ACHWN2_001096"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR8930721.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39718..39978
                     /gene="mcbA"
                     /locus_tag="ACHWN2_001097"
     CDS             39718..39978
                     /gene="mcbA"
                     /locus_tag="ACHWN2_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR8930722.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40243..>40543
                     /locus_tag="ACHWN2_001098"
     CDS             40243..>40543
                     /locus_tag="ACHWN2_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR8930723.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10095 a        10720 c         9866 g         9862 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
    15121 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
    15181 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
    15241 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
    15301 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
    15361 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
    15421 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
    15481 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
    15541 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
    15601 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
    15661 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
    15721 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
    15781 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
    15841 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
    15901 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
    15961 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
    16021 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
    16081 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
    16141 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
    16201 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
    16261 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
    16321 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
    16381 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
    16441 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
    16501 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
    16561 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
    16621 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
    16681 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
    16741 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
    16801 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
    16861 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
    16921 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
    16981 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
    17041 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
    17101 acctgcgtag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
    17161 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
    17221 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
    17281 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
    17341 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca
    17401 gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat
    17461 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact
    17521 tcgtcacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
    17581 gagtaattga ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
    17641 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
    17701 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
    17761 ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg
    17821 tcggaatccc ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa
    17881 ctttcaccac gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt
    17941 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
    18001 gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg
    18061 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
    18121 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
    18181 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa
    18241 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
    18301 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
    18361 gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc
    18421 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
    18481 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
    18541 gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga
    18601 ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg
    18661 gtgaaccctt catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag
    18721 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
    18781 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
    18841 agtggaccga cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg
    18901 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
    18961 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
    19021 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
    19081 aaaaaccgct tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag
    19141 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
    19201 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac
    19261 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
    19321 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
    19381 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
    19441 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
    19501 tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca
    19561 gacggtacag gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac
    19621 cggcatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt
    19681 cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
    19741 gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga
    19801 tatcatgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat
    19861 ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg
    19921 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
    19981 catccagttg attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga
    20041 tcagtatctc tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga
    20101 acacgccatc gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg
    20161 ctccggctcc cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg
    20221 cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat
    20281 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
    20341 aggaatgagt aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat
    20401 gccggcattc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
    20461 gcaggacgct tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg
    20521 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
    20581 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
    20641 ggcaggtaac ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta
    20701 ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
    20761 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
    20821 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg
    20881 caacagcctg gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca
    20941 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
    21001 gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc
    21061 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg
    21121 tggtccggtt ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt
    21181 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
    21241 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg
    21301 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
    21361 aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga
    21421 agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
    21481 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
    21541 cttcaccgcg ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct
    21601 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
    21661 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
    21721 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg
    21781 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
    21841 aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc
    21901 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
    21961 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
    22021 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
    22081 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
    22141 atcgcctgaa acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
    22201 tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg
    22261 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
    22321 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
    22381 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
    22441 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
    22501 atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca
    22561 gctgacctct ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat
    22621 cacatcaaat cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt
    22681 agcatagttt cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac
    22741 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    22801 cttggcgatg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    22861 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    22921 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    22981 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    23041 tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    23101 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    23161 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    23221 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    23281 cgcctccttc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    23341 ctggaacgct ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat
    23401 ctggcgaaat acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    23461 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat
    23521 atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta
    23581 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    23641 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    23701 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    23761 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    23821 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    23881 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    23941 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    24001 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    24061 taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata
    24121 ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc
    24181 agccgtcgtc accccgacag tatcggggtg acggataatc acttactcga tccctttact
    24241 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    24301 aatgcgggtc gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc
    24361 ctgatacagt tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt
    24421 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    24481 ccctttttcc ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag
    24541 caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact
    24601 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    24661 accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    24721 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc
    24781 accgacctcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    24841 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa
    24901 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    24961 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    25021 caactcagca atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcctg
    25081 ggtcgccgcc gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc
    25141 cagatccgcc atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    25201 catggtgctg tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag
    25261 gttgttggtc ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag
    25321 aagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    25381 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    25441 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    25501 caaagcatag atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat
    25561 caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    25621 gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat
    25681 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    25741 gccgaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    25801 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    25861 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    25921 tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca
    25981 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    26041 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    26101 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    26161 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg
    26221 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    26281 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    26341 ttaacagtgc agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc
    26401 tgccgatcgg cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg
    26461 agcataagaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg
    26521 ctgcgggcga accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    26581 atgcactcta catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg
    26641 gcctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg
    26701 agaaagtacc ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    26761 agccagacgg cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt
    26821 ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc
    26881 agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg
    26941 ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta
    27001 tttgccagac cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc
    27061 cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc
    27121 atacgtagcg caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt
    27181 accgcccatg ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc
    27241 acgttactga tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa
    27301 tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg
    27361 ataaacatcg ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc
    27421 gccagcagca caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc
    27481 agcgccgtca gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac
    27541 tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac
    27601 aacagcagga gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg
    27661 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    27721 agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc
    27781 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    27841 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    27901 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    27961 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    28021 ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat
    28081 aactgaaaaa acgatcgcct tgcagcgtgc gtaaaaaagg caggctcatt tggcaccgtg
    28141 tttctgggta aacaaacgaa aagcgtcgag cgtctcttca ctaacatggt gctccatgcc
    28201 ttccgcgtcg cgacgggcga tttccggact gacgcccaac accaacaaga aattttcgac
    28261 tatctgatgt cgctcgcggc tttcctgcgc cagcttctct ccttctgccg ttaaaaacac
    28321 gccacgccag gggatcattt caatcagccc catggttgcc agccgcttaa gcattttagc
    28381 caccgtcggt tgcgaaactc ccagacgagc agccatgtcc acctgacgag cttcccccac
    28441 ttccctgatc aagtcagaaa tcagctcaac gtaatcatca ataagctcgc gccgatgcgc
    28501 ctctctgacc tggcggaacc cttcaacgtg ctcttccacg ttcactaact gcgtcacttt
    28561 ttttgctatt ggcgtacctg cgcgacgact cattgtgctt cctcattatg gtgacgcata
    28621 catagcgttc tcatttcgtg cgcacattgt aaaccagagt tgcgaaggta caaaaaatta
    28681 acgttttagc aatagctata taatatagcc tgtgctatat ctgtatgtaa tgcaatcatc
    28741 cctcaaggat cgacgggatt agcaagtcag gaggtcttat gaatgagttc aagaggtgta
    28801 tgcgcgtgtt tagtcattct ccctttaaag tacggttaat gctgctctct atgttgtgcg
    28861 atatggtcaa caacaaaccg cagcaagata aaccttcaga taaatagcgg cgtcgcggta
    28921 cgccgcttca ctcctgcttt tatgcaggca taacgcgttt tggtctgaaa aaaaccactt
    28981 tttgtcggat ttgcaatccc cttcgcaaaa gatttgttcg tcagtagttg acctgaacgg
    29041 cggctcgctc tatcttcttg cagccctgcg tatattgcgg ctcgcggatg cggacccctt
    29101 tcccctcttc acgcactctt gcaggtattg acccttgacg ccagggtaag cacatggcgt
    29161 ttgttacgat agtggcatat gaatttaacc ctcaaagaat cgcttgttac ccgtagccgg
    29221 gtatttagcc cgtggactgc gttctacttt ttacagtcgc tattaattaa cctcggctta
    29281 ggttacccct tcagtttgct ctacaccgct gcgtttacgg ctattttgct tttgctatgg
    29341 cgaacattgc ctcgcgtaca aaaagttctg gtcggtgtca gttcgctggt ggcggcttgt
    29401 tatttccctt ttgctcaggc ctacggcgcg cctaatttca atacattgct ggcattgcac
    29461 tccaccaata tggaagagtc gaccgaaatc ctgacgattt ttccgtggta cagctacctg
    29521 gtcggcttat ttatttttgc gctcggcgta atagcaatca ggcgaaaaaa agagaatgaa
    29581 aaagcgcgct ggaatacctt cgacagcctg tgtctggtat tcagtgtggc gacatttttc
    29641 gttgctcccg tgcaaaacct ggcctggggt ggcgtattta aactgaaaga tactggctat
    29701 ccggtatttc gttttgctaa ggatgtcatc gtcaataata acgaggtgat tgaagagcaa
    29761 gaacggatgg caaaactttc cggaatgaaa gatacctgga cggtcactgc cgttaagccg
    29821 aagtatcaga cctatgtggt agtgatcggt gaaagcgcgc gtcgcgatgc cctcggtgcc
    29881 tttggcggtc actgggacaa taccccgttt gccagcagcg ttaacggttt gatatttgct
    29941 gactacattg ctgccagtgg ctccacgcag aaatcgcttg gcttaacgct caatcgcgta
    30001 gtcgatggca aaccacagtt tcaggataac tttgtcaccc tggcaaatcg cgcgggcttc
    30061 cagacctggt ggttttccaa ccagggccaa atcggcgaat acgatactgc tatcgccagc
    30121 atcgccaaac gtgcagatga agtgtacttc ctgaaagaag gtaattttga agcagataaa
    30181 aacacgaaag acgaagcgtt actggatatg accgctcaag tgctggcgca agagcactcg
    30241 caaccgcagc tgattgttct gcatctgatg ggctcgcatc cgcaggcctg cgacaggaca
    30301 caaggcaaat acgaaacctt tgtgcaatcg aaagaaacgt cgtgctatct ctataccatg
    30361 acgcaaacgg acgatttact gcgcaagctg tacgatcagt tacgcaacag cggcagcagc
    30421 ttctctctgg tttacttttc tgaccacggt ctggccttta aagagcgcgg caaagacgtg
    30481 caataccttg cccatgatga taaatatcag caaaatttcc aggtgccttt tatggtcatt
    30541 tccagcggcg ataaagcgca tcgggtgatt aaagcccgcc gctcagccaa tgacttctta
    30601 ggctttttct cgcagtggac gggaattaaa gcgaaggaaa taaatatcaa atacccgttt
    30661 atatctgaga agaaagccgg gccgatatac atcaccaact tccagttaca gaaggtggat
    30721 tacaaccatc tcggaaccga tattttcgac ccgaaacctt aaaaacaaaa atccgccccg
    30781 agaggcggat ttttatatca ccaaagtgat tagaagcggt aaccaacacc ggcaatccag
    30841 gtgcctacgt caacgctacg aatacggctc tgctcgtaag agaagtccag agcaacgttt
    30901 tccatcgggt tgaactgcag accagcaccg taggagaaac cgtagtcgct ggtgtcgtgt
    30961 ttgtaggtcg ggtattcagt ggtctggaat ttaccataac ccacacccac tacaccgtag
    31021 atgcttgccc agtcgttaat gcggtaagcc ggaccagcag tgatgccgta gtactggttt
    31081 ttgttgtagt caccagagct tgcagtacgg cttttctcgg tgtaagtgaa agaaccgatc
    31141 acacccagcg ggctgttgtc ttcttcatag cggtatttca ggttgaaacc gcccattttg
    31201 ttcatttggc cctgagcgtc gctctgtgcg taaccgccag ttacggtaga agtcgcagct
    31261 acggaagtac ctgcggtgaa agccagaact gcggccagtg ctgaaagaca tgcaattttt
    31321 ttcataacca cctcaaatgt gattcaaata agtcctaagt tttaaatata tcaaaaatta
    31381 atgggaaact cttcgcgatt tgtgatgtct aacgggccat ttcatgtaac agaacgtttc
    31441 catacaccgc tatccatcta aatttaaatc actttttcag agaactgcgt aagtattacg
    31501 catgttttcc ctgtcattca tccagattat tcctaatcac cagactaatg attccatcaa
    31561 tcctggcgca ttttagtcaa aacgggggaa aattttttca acaaatgctc aaccagcatt
    31621 gggtatatcc agtacactcc acgctttact taagtctaga tatttgtggg agaaaggatg
    31681 cctggttcat tacgtaaaat gccggtctgg ttaccaatag tcatattgct cgttgccatg
    31741 gcgtctattc agggtggagc ctcgttagct aagtcacttt ttcctctggt gagcgcaccg
    31801 ggtgtcactg cgctgcgtct ggcattagga acgctgatcc tcatcgcgtt ctttaagcca
    31861 tggcgactgc gctttgccaa agagcaacgg ttaccgctgt tgttttacgg cgtttcgctg
    31921 ggtgggatga attatctttt ttatctttct attcagacag taccgctggg tattgcggtg
    31981 gcgctggagt tcaccggacc actggcggtg gcgctgttct cttctcgtcg cccggtagat
    32041 ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc tgctaccgct ggggcaagac
    32101 gtttcccatg tcgatttaac cggctgtgcg ctggcactgg gggccggggc ttgttgggct
    32161 atttacattt taagtgggca acgcgcagga gcggaacatg gccctgcgac ggtagcaatt
    32221 ggttcgttga ttgcagcgtt aattttcgtg ccaattggag cgcttcaggc tggtgaagca
    32281 ctctggcact ggtcggttat tccattgggt ctggctgtcg ttattctctc gaccgctctg
    32341 ccttattcgc tggaaatgat tgccctcacc cgtttgccaa cacggacatt tggtacgctg
    32401 atgagcatgg aaccggcgct ggctgccgtt tccgggatga ttttcctcgg agaaacactg
    32461 acacccatac agctgctggc gctcggcgct atcatcgccg cttcaatggg gtctacgctg
    32521 acagtacgca aagagagcaa aataaaagaa ttagacatta attaaattta catttctgta
    32581 tggttatgca taaccatgca gaatttctcg ctacttttcc tctacaccgt atttatatat
    32641 cgaattatgc aaaagcatat ttattccgaa aattcctggc gagcagataa ataagaattg
    32701 ttcttatcaa tatatctaac tcattgaatc tttattagtt ttgtttttca tgcttgttac
    32761 cactattagt gtgataggaa cagccagaat agcggaacac atagccggtg ctatacttag
    32821 tctcgttaat tactgggaca taacatcaag aggatatgaa attatgagta ccgctaaatt
    32881 agttaaatca aaagcgacca atctgcttta tacccgcaac gatgtctccg acagcgagaa
    32941 aaaagcaaca gtagagttgc tgaatcgcca ggttatccag tttattgatc tttctttgat
    33001 taccaaacaa gcgcactgga acatgcgcgg cgctaacttc attgccgtac atgaaatgct
    33061 ggatggcttc cgcaccgcac tgatcgatca tctggatacc atggcagaac gtgccgtgca
    33121 gctgggcggt gtagctctgg ggaccactca agttatcaac agcaaaaccc cgctgaaaag
    33181 ttacccgctg gacatccaca acgttcagga tcacctgaaa gaactggctg accgttacgc
    33241 aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg aaagatgacg acaccgcaga
    33301 tatcctgacc gccgcgtctc gcgacctgga taaattcctg tggtttatcg agtctaacat
    33361 cgaataaatc catcgctgat ggtgcagaac tttagtaccc gataaaagcg gcttcctgac
    33421 aggaggccgt tttgttttgc agcccacctc aacgcactta tttagtgcat ccatctgcta
    33481 tccccagctg attaagtaaa ttttttgtat ccacatcatc acacaatcgt tacataaaga
    33541 ttgttttttc atcaggtttt acgctaaata atcactgtgt tgagtgcaca attttagcgc
    33601 accagattgg tgccccagaa tggtgcatct tcagggtatt gccctataaa tcgtgcatca
    33661 cgtttttgcc gcatctcgaa aaatcaatga gttgcaaaat tggcacgatt ttttcatata
    33721 tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa aggaaatgct atgaagtctg
    33781 tattaaaagt ttcactggct gcactgaccc tggcttttgc ggtttcttct catgccgcgg
    33841 ataaaaaatt agttgtcgcg acggataccg ccttcgttcc gtttgaattt aaacagggcg
    33901 ataaatatgt gggctttgac gttgatctgt gggctgccat cgctaaagag ctgaagctgg
    33961 attacgaact gaagccgatg gatttcagtg ggatcattcc ggcactgcaa accaaaaacg
    34021 tcgatctggc gctggcgggc attaccatca ccgacgagcg taaaaaagcg atcgatttct
    34081 ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa agctaacaat aacgatgtga
    34141 aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa gagcggtact ggctccgttg
    34201 attacgcgaa agcaaacatc aaaactaaag atctgcgtca gttcccgaac atcgataacg
    34261 cctatatgga actgggcacc aaccgcgcag acgccgttct gcacgatacg ccaaacattc
    34321 tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc ggtaggtgac tctctggaag
    34381 cgcagcaata cggtattgcg ttcccgaaag gtagcgacga gctgcgtgac aaagtcaacg
    34441 gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga aatctacaaa aaatggttca
    34501 gtactgaacc gaaataataa cgctacacct gtaaagcgca ctggcagttc cctctccccc
    34561 ctggggagag gattagggtg aggggcgcaa acccgctccg gggccattaa ttaccctgaa
    34621 tttgattatt tacaccacgg taacaggaac aacatatgca gtttgactgg agtgccatct
    34681 ggcctgccat tccgcttctg attgaaggtg ccaaaatgac cctgtggatt tcggtcctcg
    34741 gtctggctgg cggtctggta atcggattgc tggcaggttt tgcacgcacc ttcggtggtt
    34801 ggatagccaa ccacgtcgcg ctggtcttta ttgaagtgat ccgcggcaca cctatcgtcg
    34861 tccaggtgat gtttatctat ttcgccctgc cgatggcgtt taacgactta cgcatcgacc
    34921 catttactgc ggcggtggtc accatcatga tcaactccgg cgcgtacatt gcggaaatca
    34981 cgcgtggtgc ggtgctgtct atccacaaag gttttcgtga agcaggactg gcgctcggtc
    35041 tttcacgttg ggaaaccatt cgctacgtca ttttaccgct ggcactgcgt cgtatgctgc
    35101 cgccgctggg taaccagtgg atcatcagca ttaaagacac ctcgctgttt attgttatcg
    35161 gcgtggcgga actgacccgt caggggcaag aaattattgc cggtaacttc cgcgcccttg
    35221 agatctggag cgccgtggcg gtgttctatc tgattattac cctggtgctg agctttattc
    35281 tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa tttaaaaacg tctccaagca
    35341 ctttggccca acccaggtgc tgcacaatat cgatttgaac attgcccagg gcgaagtcgt
    35401 ggtgattatc gggccgtccg gttccggtaa atcgaccctg ctgcgctgca tcaacaaact
    35461 ggaagaaatc acctccggcg atctgattgt cgatggcctg aaggtcaacg atccgaaagt
    35521 tgacgagcgc ctgattcgcc aggaagcagg tatggtgttc cagcagtttt acctcttccc
    35581 gcatctgacg gcgctgggaa acgtcatgtt tggtccgcta cgcgtgcgtg gcgcgaacaa
    35641 agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa gtcggtctgg cagaacgtgc
    35701 acatcactac ccttccgaac tttctggtgg tcaacagcag cgtgtggcga ttgcccgcgc
    35761 gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg acttccgctc ttgacccgga
    35821 actgcgccat gaagtgctga aggttatgca ggatctggct gaagaaggga tgacgatggt
    35881 gatcgtgacc cacgaaatcg gttttgccga gaaagtagct tcgcggctga tctttatcga
    35941 caaaggccgg attgcggaag atggcaatcc gcaggtgttg atcaagaacc cgccgagcca
    36001 gcgcttgcag gaatttttgc agcacgtctc ttaataagac acattgcctg atgcgctacg
    36061 cttatcaggc ctacaggata tctggcaact tattaaaatt gcatgaactt gtaggacgga
    36121 taaggcgttc acgccgcatc cggcaaaaaa gcccgcacgt tgtcagcaac ctgcttaata
    36181 tcccttcctc cctttcaccc gaaagggagg cacaccagat tcctctcatt taaaatcgcc
    36241 cctcccccag catctatact tatctttttg ctctgttttc tcactggagg agtcatgcgg
    36301 tggatcctgt tcatcctctt ctgcctgctg ggcgcacctg cccacgcggt atccataccc
    36361 ggcgttacaa ccacaacgac aacggactca acgactgaac cggccccgga accggatatc
    36421 gaacaaaaaa agcggcctat ggcgcactgg cggatgtgct ggataacgac acctcgcgta
    36481 aagagttgat cgaccagttg cgtactgtcg ccgcgacgcc ccctgcggaa ccggtcccga
    36541 agatcgtgcc gccgacgctg gttgaagagc aaaccgtgct gcaaaaggtc accgaagtca
    36601 gccgccatta tggtgaagcc ctttccgccc gcttcgggca actttatcgc aatatcaccg
    36661 gctccccgca taagccattt aatccacaaa ccttcagcaa tgcgctgagc catttttcaa
    36721 tgttagcggt attagtgttt ggtttttact ggctgattcg cctgtgcgca ctgccgctat
    36781 atcgcaaaat gggtcagtgg gcgaggcaaa aaaatcgtga gcgcagcaac tggttgcagc
    36841 ttccggcgat gattattggg gcgtttatta tcgacctgct gttactggcc ctgacattat
    36901 ttgtcggcca ggtattaagc gacaacctga atgcaggcag tcgcaccatc gctttccaac
    36961 aaagtttgtt tctcaacgcc tttgccctga ttgaattttt caaagccgta ctacgcctga
    37021 ttttttgccc aaacgtggcg gagctgcgcc cgttcacgat tcaggacgag agcgcccgtt
    37081 actggggtcg tcgcctgagc tggttaagca gcctgattgg ctatggcctg attgtggccg
    37141 tgccgattat ctctaatcag gtgaatgtac agataggtgc gctggcgaac gtcatcatta
    37201 tgctgtgcat gaccgtctgg gcgttgtacc tgatcttccg aaataaaaaa gagattaccc
    37261 agcatatgct caactttgcg gagcattcgc tggctttttt cagcctgttt atccgcgcct
    37321 ttgcgctggt gtggcactgg ctggcaagcg cctattttat catgctgttt ttcttttcgt
    37381 tgttcgatcc gggcaacagt ctgaaattta tgatgggtgc aacggtgcgc agcctggcga
    37441 ttattggtat cgcagcgttt gtttccggta tgttttcccg ctggctggcg aaaaccatca
    37501 ccctctcgcc acatactcag cgtaactatc cggagctaca aaaacggctg aatggctggc
    37561 tttcggcggc gctgaaaacg gcgcgtattc tgaccgtctg cgtggcagta atgttgctgt
    37621 tgagcgcatg gggattgttc gatttctgga actggctgca aaacggcgcg gggcagaaaa
    37681 ccgtagatat cctgatccgc atcgcactca ttcttttctt ctcggcggtt ggctggacgg
    37741 tgctcgccag tttgatcgaa aaccggctgg cttcggatat tcatggccgc ccgctaccca
    37801 gcgcccgtac gcgtaccctg ctgacgctgt tccgtaacgc gctggcggtg attatcagta
    37861 ccatcaccat catgattgtg ttgtcggaaa tcggcgtcaa tatcgcgcca ttgctggcag
    37921 gtgccggggc attaggtctg gctatctcgt ttggttcgca aacgctggta aaagatatta
    37981 tcaccggggt atttattcag tttgaaaacg gcatgaacac tggagatttg gtgactatcg
    38041 ggccgttgac cggcacagtg gaacggatgt cgattcgctc cgtgggcgtg cgccaggata
    38101 ccggggcgta tcacatcatt ccgtggtctt cgataaccac ctttgctaac ttcgtccgcg
    38161 gtattggttc ggtagtggca aactacgatg ttgatcgcca tgaagatgct gataaagcca
    38221 atcaggcact gaaagatgcg gtaacggaat tgatggaaaa cgaagaaatt cgcgggctga
    38281 ttattggtga accgaacttt gccgggattg tcggcttaag caataccgcg tttacactgc
    38341 gtgtttcgtt caccacgctg ccactcaaac agtggacggt acgctttgcc ctcgacagcc
    38401 aggtgaaaaa acatttcgac ctggcgggcg ttcgcgcgcc agtgcagact tatcaggtgc
    38461 tgcctgctcc gggcgcgacc ccggctgaac cgctgccgcc gggggagcca acgctttaac
    38521 gttggcgatt aacaaaacgg cggcgctgct cgtcgttcat aaaggtccag gcaataaagc
    38581 gactctgctt ttgcccctgg gccatctctt tttttaccac cttcaccgcg cccacgtcgg
    38641 tcagagcacg atacaacggc ggtaagtttt caccacgaga taccagcgag gtaaaccaca
    38701 tcacctgctt cgcgaagcct ttgctctctt caatcatctt tttgataaag ttgacttcac
    38761 cgccttcaca ccacaactcc tgttgctgac caccaaagtt cagtgcatcg tctttgttca
    38821 gccccaggtt acggcgttta cgctcactac ctgcccgcgc cgcagcggcg gaatcgtgga
    38881 atggcgggtt acacaaggtc gcgtcgtatt gctcgttttt gtggatgatg ccgttaaaaa
    38941 tcgccccact ctctttttgc cgacgcagac gaatggcgcg gttaagcccc ggattagcac
    39001 tgataatcgc ctgcgcactg cttaacgcct ggctgctggt ttcactaccg gtaaaacgcc
    39061 agccatattc atgtacgcca atcagcggat aaatacagtt cgcaccaacg ccgatatcca
    39121 gaatgctggc attcgctgga attgttccgc ttgcttcagc cagtaaatcg gcaaggtgat
    39181 gaatataatc cgcccggccc ggtactggtg ggcagagaaa accgtcgggg atatcccagt
    39241 tcgctacggc gtaaaaatgg gccagcaacg ccttattgag cgccttcacc gccagcggat
    39301 tggcaaagtc tacgctttgc tcccctgcgg gtgtaagcgt gaggaattgc ctgagttcag
    39361 gattgacctg acaaagcgtg gcgagatcgt agcggctgtg atgacggttg cgcggatgca
    39421 accccggttt ctgggcggac atagcattct cctttaagca gcggcgtaag atacccgcat
    39481 aacgcgacag ggtaaatatc tggctgcgtt tatcattccc caaaacctta acatggctat
    39541 ggtttatggc aattcatcat aaaaatcagc attatgcatg tcattgattt gtcttatttt
    39601 gtaaaaatat atcgttcaaa aaataagcat ttctctgtca attttttgcg tcatttgcat
    39661 cgcgcaggcg acctacactt atcaggcact acccgttgta tttatgagga tgtcattatg
    39721 aaaaagtgcc tcacactact gattgccacc gtcctgagcg gaatctctct cacggcttat
    39781 gccgcgcaac cgatgagtaa cctggacagc ggtcagctac ggcccgccgg taccgtttcg
    39841 gcaactggcg catcaaacct aagcgatctg gaggataaac tggcagaaaa agcgcgcgaa
    39901 caaggcgcga aaggttatgt cattaattcc gcaggcggaa atgaccagat gttaggtact
    39961 gcaaccatct acaaataacc ccaccaacgt aactgcattc gcctctggat gcagttttcg
    40021 cctgttttgt aatcaaaaaa catcaaatgt gaacgcaatg taaataaatg tatttctttt
    40081 tcgcgcaatg ggtgatagaa aatcgcatca agtgataatg cttatcaaaa ttattatcac
    40141 ttgcacgaac actatcacgg gattaacagt ggcatcgcat ccgcagagat gctttctcgt
    40201 ggcagtgaaa atttcaacat ataagaaaaa gtcacctgca aaatggaaaa caatcgcaat
    40261 ttccctgcca gacaatttca ttcgctcacg ttctttgccg gtctttgtat tggtattacg
    40321 cctgtggcac aggcactcgc cgccgaaggg caagctaacg cggatgacac gctggttgtc
    40381 gaagcatcaa cgccttcgct ttatgcgcca caacaatctg ccgatccgaa attctcgcgt
    40441 ccggtagcgg atactacccg cacgatgacg gtgatttctg aacaagtgat taaagatcag
    40501 ggcgcaacca accttaccga cgcgctcaaa aacgtccccg gcg
//
LOCUS       DAZSNO010000022        40385 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZSNO010000022 DAZSNO010000000
VERSION     DAZSNO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40385
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.022"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            295..381
                     /gene="leuL"
                     /locus_tag="ACHWN2_001099"
     CDS             295..381
                     /gene="leuL"
                     /locus_tag="ACHWN2_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR8930724.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            474..2045
                     /gene="leuA"
                     /locus_tag="ACHWN2_001100"
     CDS             474..2045
                     /gene="leuA"
                     /locus_tag="ACHWN2_001100"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR8930725.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            2045..3136
                     /gene="leuB"
                     /locus_tag="ACHWN2_001101"
     CDS             2045..3136
                     /gene="leuB"
                     /locus_tag="ACHWN2_001101"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR8930726.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3139..4539
                     /gene="leuC"
                     /locus_tag="ACHWN2_001102"
     CDS             3139..4539
                     /gene="leuC"
                     /locus_tag="ACHWN2_001102"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR8930727.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4550..5155
                     /gene="leuD"
                     /locus_tag="ACHWN2_001103"
     CDS             4550..5155
                     /gene="leuD"
                     /locus_tag="ACHWN2_001103"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR8930728.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(5204..6382)
                     /gene="setA"
                     /locus_tag="ACHWN2_001104"
     CDS             complement(5204..6382)
                     /gene="setA"
                     /locus_tag="ACHWN2_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR8930729.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(6484..6615)
                     /gene="sgrT"
                     /locus_tag="ACHWN2_001105"
     CDS             complement(6484..6615)
                     /gene="sgrT"
                     /locus_tag="ACHWN2_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR8930730.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6704..8359
                     /gene="sgrR"
                     /locus_tag="ACHWN2_001106"
     CDS             6704..8359
                     /gene="sgrR"
                     /locus_tag="ACHWN2_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR8930731.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8393..8492
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8523..9506
                     /gene="thiB"
                     /locus_tag="ACHWN2_001107"
     CDS             8523..9506
                     /gene="thiB"
                     /locus_tag="ACHWN2_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR8930732.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9482..11092
                     /gene="thiP"
                     /locus_tag="ACHWN2_001108"
     CDS             9482..11092
                     /gene="thiP"
                     /locus_tag="ACHWN2_001108"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR8930733.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11076..11774
                     /gene="thiQ"
                     /locus_tag="ACHWN2_001109"
     CDS             11076..11774
                     /gene="thiQ"
                     /locus_tag="ACHWN2_001109"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR8930734.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11888..12652)
                     /locus_tag="ACHWN2_001110"
     CDS             complement(11888..12652)
                     /locus_tag="ACHWN2_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR8930735.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12738..13616)
                     /gene="araC"
                     /locus_tag="ACHWN2_001111"
     CDS             complement(12738..13616)
                     /gene="araC"
                     /locus_tag="ACHWN2_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR8930736.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13955..15655
                     /gene="araB"
                     /locus_tag="ACHWN2_001112"
     CDS             13955..15655
                     /gene="araB"
                     /locus_tag="ACHWN2_001112"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR8930737.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15666..17168
                     /gene="araA"
                     /locus_tag="ACHWN2_001113"
     CDS             15666..17168
                     /gene="araA"
                     /locus_tag="ACHWN2_001113"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR8930738.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17368..18063
                     /gene="araD"
                     /locus_tag="ACHWN2_001114"
     CDS             17368..18063
                     /gene="araD"
                     /locus_tag="ACHWN2_001114"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR8930739.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18138..20489
                     /gene="polB"
                     /locus_tag="ACHWN2_001115"
     CDS             18138..20489
                     /gene="polB"
                     /locus_tag="ACHWN2_001115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR8930740.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20654..23560
                     /gene="rapA"
                     /locus_tag="ACHWN2_001116"
     CDS             20654..23560
                     /gene="rapA"
                     /locus_tag="ACHWN2_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR8930741.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23572..24231
                     /gene="rluA"
                     /locus_tag="ACHWN2_001117"
     CDS             23572..24231
                     /gene="rluA"
                     /locus_tag="ACHWN2_001117"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR8930742.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24348..25163)
                     /gene="djlA"
                     /locus_tag="ACHWN2_001118"
     CDS             complement(24348..25163)
                     /gene="djlA"
                     /locus_tag="ACHWN2_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR8930743.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25418..27772
                     /gene="lptD"
                     /locus_tag="ACHWN2_001119"
     CDS             25418..27772
                     /gene="lptD"
                     /locus_tag="ACHWN2_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR8930744.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27825..29111
                     /gene="surA"
                     /locus_tag="ACHWN2_001120"
     CDS             27825..29111
                     /gene="surA"
                     /locus_tag="ACHWN2_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR8930745.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29111..30100
                     /gene="pdxA"
                     /locus_tag="ACHWN2_001121"
     CDS             29111..30100
                     /gene="pdxA"
                     /locus_tag="ACHWN2_001121"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR8930746.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30097..30918
                     /gene="rsmA"
                     /locus_tag="ACHWN2_001122"
     CDS             30097..30918
                     /gene="rsmA"
                     /locus_tag="ACHWN2_001122"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR8930747.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30921..31298
                     /gene="apaG"
                     /locus_tag="ACHWN2_001123"
     CDS             30921..31298
                     /gene="apaG"
                     /locus_tag="ACHWN2_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR8930748.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31305..32147
                     /gene="apaH"
                     /locus_tag="ACHWN2_001124"
     CDS             31305..32147
                     /gene="apaH"
                     /locus_tag="ACHWN2_001124"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR8930749.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32225..32704)
                     /gene="folA"
                     /locus_tag="ACHWN2_001125"
     CDS             complement(32225..32704)
                     /gene="folA"
                     /locus_tag="ACHWN2_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR8930750.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32895..34757)
                     /gene="kefC"
                     /locus_tag="ACHWN2_001126"
     CDS             complement(32895..34757)
                     /gene="kefC"
                     /locus_tag="ACHWN2_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR8930751.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREA
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34750..35280)
                     /gene="kefF"
                     /locus_tag="ACHWN2_001127"
     CDS             complement(34750..35280)
                     /gene="kefF"
                     /locus_tag="ACHWN2_001127"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR8930752.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35328..36722)
                     /locus_tag="ACHWN2_001128"
     CDS             complement(35328..36722)
                     /locus_tag="ACHWN2_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8930753.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(36781..37068)
                     /gene="fixX"
                     /locus_tag="ACHWN2_001129"
     CDS             complement(36781..37068)
                     /gene="fixX"
                     /locus_tag="ACHWN2_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR8930754.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37065..38351)
                     /locus_tag="ACHWN2_001130"
     CDS             complement(37065..38351)
                     /locus_tag="ACHWN2_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR8930755.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38402..39343)
                     /locus_tag="ACHWN2_001131"
                     /pseudo
     CDS             complement(38402..39343)
                     /locus_tag="ACHWN2_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(39358..40128)
                     /locus_tag="ACHWN2_001132"
     CDS             complement(39358..40128)
                     /locus_tag="ACHWN2_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR8930756.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9733 a        11147 c        10587 g         8918 t
ORIGIN      
        1 caaaacatat aattcaatac aaatcatcag gataggtttt gcaacgcgtg cattttgtcc
       61 cctttttcct cgttgattag atgcaaaaat ttatgctgaa atatgtcaac cgatgaaaag
      121 cgtcggtagt taagcagaaa ttaatatcgc ttactttaac caccgtagca caattagcta
      181 attttacgga tgcagaactc acgctggcgg gacgttttta ttgcgtcagg gttgacatcc
      241 gtttttgtat ccagtaactc taaaagcata tcgcattcat ctggagctga tttaatgact
      301 cacatcgttc gctttatcgg tctactacta ctaaacgcat cttctttgcg cggtagacga
      361 gtgagcggca tccagcatta agccagcacg cagtcaaaca aaaaacccgc gccattgcgc
      421 gggttttttt atgcccgaag cgaggcgctc taaaagagac aaggacccaa accatgagcc
      481 agcaagtcat tattttcgat accacattgc gcgacggtga acaggcgtta caggcaagct
      541 tgagtgtgaa agaaaaactg caaattgcgc tggcccttga gcgtatgggt gttgacgtga
      601 tggaagtcgg tttccccgtc tcttcgccgg gcgattttga atcggtgcaa accatcgccc
      661 gccaggttaa aaacagccgc gtatgtgcgt tagctcgctg cgtggaaaaa gatatcgacg
      721 tggcggccga atccctgaaa gtcgccgaag ccttccgtat tcataccttt attgctacct
      781 ccccaatgca catcgccacc aagctgcgca gcaccctaga cgaggtgatc gaacgcgcta
      841 tctatatggt gaaacgcgcc cgtaattaca ccgatgatgt tgaattttct tgcgaagatg
      901 ccgggcgtac acccattgcc gatctggcgc gagtggtcga agcggcgatt aatgccggtg
      961 ccaccaccat caacattccg gacaccgtgg gctacaccat gccgtttgag ttcgccggaa
     1021 tcatcagcgg cctgtatgaa cgcgtaccta acatcgacaa agccattatt tccgtacata
     1081 cccacgacga tttaggcctg gcggtcggca actcactggc ggcggtacat gccggtgcac
     1141 gccaggtgga aggtgcaatg aacgggatcg gcgagcgtgc cggtaactgt tcgctggaag
     1201 aagtcatcat ggcgattaaa gttcgtaagg atattctcaa cgtccacacc gccattaatc
     1261 accaagagat atggcgcacc agccagttag ttagccagat ttgtaatatg ccgatcccgg
     1321 caaacaaagc cattgttggc agcggcgcat tcgctcactc ctccggtatc caccaggatg
     1381 gcgtgctgaa aaaccgcgaa aactacgaaa tcatgacacc agaatctatt ggtctgaacc
     1441 aaatccagct gaatctgacc tctcgttcgg ggcgtgcggc ggtgaaacat cgcatggatg
     1501 agatggggta taaagaaagt gaatataatt tagacaattt gtacgatgct ttcctgaagc
     1561 tggcggacaa aaaaggtcag gtgtttgatt acgatctgga ggcgctggcc ttcatcggta
     1621 agcagcaaga agagccggag catttccgtc tggattactt cagcgtgcag tctggctcta
     1681 acgatatcgc caccgccgcc gtcaaactgg cctgtggcga agaagtcaaa gcagaagccg
     1741 ccaacggtaa cggtccggtc gatgccgtct atcaggcaat taaccgcatc actgactata
     1801 acgtcgaact ggtgaaatac agcctgaccg ccaaaggtca cggtaaagat gcgctgggtc
     1861 aggtggatat tgtcaccaac tacaacggtc gccgcttcca cggcgtcggc ctggccaccg
     1921 atattgtcga gtcctccgcc aaagccatgg tgcacgtact gaacaatatc tggcgtgccg
     1981 cagaagtcga aaaagagttg caacgcaaaa ctcaacacaa cgaaaacaac aaggaaaccg
     2041 tgtgatgtcg aagaattacc atattgccgt attgccggga gacggtattg gtccggaagt
     2101 gatgacccag gcgctgaaag tgctgaatgc cgtgcgcaac cgctttgcga tgcgcatcac
     2161 caccagccat tacgatgtag gcggcgcagc cattgataac cacgggcaac cactgccgcc
     2221 tgcgacggtt gaaggttgtg agcaagccga tgccgtgctg tttggctcgg taggcggccc
     2281 gaagtgggaa catttaccac cagaccagca accagaacgc ggcgcgctgc tgccgctgcg
     2341 taagcacttc aaattattca gcaacctgcg cccggcaaaa ctgtatcagg ggctggaagc
     2401 attctgtccg ctgcgtgcag acattgccgc taacggcttc gacatccagt gcgtgcgcga
     2461 actgaccggc ggcatctatt tcggtcagcc aaaaggccgc gaaggtagcg gacaatatga
     2521 aaaagcgttt gataccgagg tgtatcaccg ttttgagatc gagcgtattg cccgcatcgc
     2581 gtttgaatct gctcgtaaac gtcgccacaa agtcacctca atcgacaaag ctaacgtgct
     2641 gcaatcctct attttatggc gtgaaatcgt caacgagatc gccacggaat acccggatgt
     2701 cgaactggcg catatgtaca tcgacaacgc caccatgcag ctgattaaag atccatcaca
     2761 gtttgacgtt ctgctgtgct ccaacctgtt tggcgacatt ctgtctgacg agtgcgcaat
     2821 gatcactggc tcgatgggca tgttgccttc cgccagcctg aacgagcaag gttttggact
     2881 gtatgaaccg gcgggcggct cggcaccaga tatcgcaggc aaaaacatcg ccaacccgat
     2941 tgcacaaatc ctgtcgctgg cactgctgct gcgttacagc ctggatgccg atgatgcggc
     3001 ttccgccatt gaacgcgcca ttaaccgtgc attagaagaa ggcattcgca ccggggattt
     3061 agcccgtggc gctgccgccg ttagtaccga tgaaatgggc gatatcattg cccgctatgt
     3121 ggcagaaggg gtgtaatcat ggctaaaacg ttatacgaaa aattattcga cgctcacgta
     3181 gtgtacgaag ccgaaaacga aaccccgctg ttatatatcg accgccacct ggtgcatgaa
     3241 gtgacctcac cgcaggcgtt cgatggtctg cgcgcccacg gtcgcccggt acgtcagccg
     3301 ggcaaaacct tcgccactat ggatcacaac gtctctaccc agaccaaaga cattaatgcc
     3361 tgtggtgaaa tggcgcgcat ccagatgcag gagctgatta aaaactgcaa agaatttggc
     3421 gtcgagctgt atgacctgaa tcacccgtat caggggatcg tccacgtaat ggggccggaa
     3481 cagggcgtca ccttgccggg gatgaccatt gtctgcggcg actcgcatac cgccacccac
     3541 ggcgcgtttg gcgcactggc ctttggtatc ggcacttccg aagttgaaca cgtactggca
     3601 acacaaaccc tgaaacaggg ccgtgcgaag accatgaaaa ttgaagtcca gggcaaagcc
     3661 gcgccgggca ttaccgcaaa agatatcgtg ctggcaatta tcggtaaaac tggcagcgca
     3721 ggcggcaccg ggcatgtggt ggagttttgc ggcgaagcaa ttcgtgattt aagcatggaa
     3781 ggtcgtatga ccctgtgcaa tatggcgatc gaaatgggcg caaaagccgc tctggttgct
     3841 cctgacgaaa ccacctttaa ctatgtcaaa ggccgtctgc atgcgccgaa aggcaaagat
     3901 ttcgacgacg ccgttgccta ctggaaaacc ctgcaaaccg acgaaggcgc aactttcgat
     3961 accgttgtca ctctgcaagc agaagaaatt tcaccgcagg tcacctgggg caccaatccc
     4021 ggccaggtga tttctgtgaa cgacaatatt cccgatccgg cttcgtttgc cgatccggtt
     4081 gaacgtgcgt cggcagaaaa agcgctggcc tatatggggc tgaaaccggg tattccgctg
     4141 accgaagtgg ctatcgacaa agtgtttatc ggttcctgta ccaactctcg cattgaagat
     4201 ttacgcgcgg cagcggagat cgccaaaggg cgaaaagtcg cgccaggcgt gcaggcactg
     4261 gtggttcccg gctctggccc ggtaaaagcc caggcggaag cggaaggtct ggataaaata
     4321 tttattgaag ccggttttga atggcgcttg cctggctgct caatgtgtct ggcgatgaac
     4381 aacgaccgtc tgaatccggg cgaacgttgt gcatccacca gcaaccgtaa ctttgaaggc
     4441 cgccaggggc gcggcgggcg cacgcatctg gtcagcccgg caatggctgc cgctgctgct
     4501 gtgaccggac atttcgccga cattcgcaac attaaataag gagcacacca tggcagagaa
     4561 atttatcaaa cacacaggcc tggtggttcc gctggatgcc gccaatgtcg ataccgatgc
     4621 aatcatcccg aaacagtttt tgcagaaagt gacccgtacg ggttttggcg cgcatctgtt
     4681 taacgactgg cgttttctcg atgaaaaagg ccaacagcca aacccggact tcgtgctgaa
     4741 cttcccgcag tatcagggcg cttccatttt gctggcacga gaaaacttcg gctgtggctc
     4801 ctcgcgtgag cacgcgccct gggcattgac cgactacggt tttaaagtgg tgattgcgcc
     4861 gagttttgct gacatcttct acggcaatag ctttaacaac cagctgctgc cggtgaaatt
     4921 aagcgatgca gaagtggacg aactgtttgc gctggtgaaa gccaatccgg ggatccattt
     4981 cgacgtggat ctggaagcgc aagaggtgaa agcgggagag aaaacctatc gctttaccat
     5041 cgatgccttc cgccgccact gcatgatgaa tggtctggac agcatcggcc tgaccctgca
     5101 gcacgacgac gctattgcgt cttatgaagc aaaacaacct gcgtttatga attagacccc
     5161 ttgcccggtc aaatgaccgg gctttccgct atcgtccacg tcatcaaacg tctttaacct
     5221 ttgcggttaa aaataatgcg acaacagaaa taatcgcaat tacccagtag acagcaaagt
     5281 gcccccaact ttgtgcaatt gctccctgaa taacaccagc cagaattacc ccggtagaga
     5341 tagtgttggt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa tcctgaaacc
     5401 atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt tgcagcgtca
     5461 tcaacgccat acggctatga aagaaaatca atccagtgta aaacagcact cctgccacca
     5521 ctgcaatgac catcattcgc cgcttaccat aacgtttgac atagtagcca gccagaatca
     5581 ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt ttgtctggca
     5641 atcctaaatc gctactgatc cacaatggca tatcaataat gtacatagtg ttgcaggtcc
     5701 acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag tcaccttgca
     5761 ttgataaagc attttccgac ggcagttcta cccgcgccac agacagaagc ataaatgcaa
     5821 tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcact gtaaagccgt
     5881 aattcaacgc cagcataaag gccagcggtg gaccgattac ccatgccaga gaaagctgcg
     5941 cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgcg tattcccgcg
     6001 ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc acaccacagg
     6061 tgataagcgt cagataatga cgattaaatg caaacagtag cgcattgcca atagccatca
     6121 aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt tttgccaacc
     6181 agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg atccagaaag
     6241 gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt agcgccccgg
     6301 ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc atacggcgag
     6361 ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata aaaaaaacca
     6421 gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca tgatgcaggc
     6481 aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca tggttaatcg
     6541 ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa aatagtgctg
     6601 ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg acttaatata
     6661 gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat ctgctcgtct
     6721 gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg acacaacgct
     6781 caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc tgctcaacac
     6841 catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta aacgctcgcg
     6901 tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag acctgctgga
     6961 gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg tgcggcaaat
     7021 gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc gcgtgctcta
     7081 ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg aaacccatat
     7141 tgcccggcaa atcttcagtt cgctaacgcg cataaatgag gaaaatgggg aactggaagc
     7201 agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct ttttgcgtcc
     7261 aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg cctctttaaa
     7321 acgaatcaat acgctgccgc tctattcgca tattacggac attgtgtcgc cgacgccctg
     7381 gacgctggat atccatctca cgcagccgga ccgctggttg ccgttactgc tggggcaagt
     7441 tccggcgatg atcctgccgt gcgaatggga aaccctcagt aactttgcca gccatcctat
     7501 tggcaccggt ccgtatgcgg tgattcgcaa cagcaccaat caactgaaaa ttcaggcatt
     7561 cgatgacttc ttcggttacc gggcattaat cgacgaagtt aacgtctggg ttctgccgga
     7621 aattgccgac gagccagccg gtggtctgat gctaaaaggt ccacagggcg agaaaaaaga
     7681 gattgaaagc cgcctggagg aaggttgcta ctatttactg ttcgacagcc gcacccatcg
     7741 cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa ctaatctggt
     7801 ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac tgctcccccg
     7861 ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa gcctcaccct
     7921 gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc agcagattct
     7981 ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt ggcatgaagg
     8041 agagatcgag agcgatatct ggcttaatag cgccaacttt acgctgccgc tggatttttc
     8101 gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga tcgactggca
     8161 agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt gccagcaact
     8221 ggtcgccagt aaagcaatgg tgccgcttat ccaccactgg ctgatcattc agggacaacg
     8281 cagtatgcgc ggcctgcgta tgaataccct cggctggttc gattttaaat cagcgtggtt
     8341 tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa cgccgttctc
     8401 aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg atccggataa
     8461 cgccggcgaa gggatttgag gctccttctc aagtcctttg ccactctttt ttgaggtgca
     8521 aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt ttcgctaaac
     8581 ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct ggtccaaaga
     8641 tcaaaaaagc ttttgaagcc aactgtaact gcgaactgaa actggtggcg ctggaagatg
     8701 gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc gatgtggtgc
     8761 tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt gccaaaagcg
     8821 gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact ttcgtcccgt
     8881 ttgattacgg ctatttcgcc ttcgtctatg acaagaacaa actgaaaaac ccgccgcaaa
     8941 gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag gatccgcgta
     9001 ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc gatgacgccc
     9061 cacaagcctg gcaaaaactg gcgaagaaaa ccgtcacggt caccaaaggc tggagcgaag
     9121 cctacggcct gtttttaaaa ggtgaaagcg atctggtact gagttacacc acctctccgg
     9181 cttatcacat tctcgaagag aaaaaagata actacgccgc cgcgaacttt agcgaaggtc
     9241 actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg gagctggcgc
     9301 aaaaattcct ccagtttatg gtttctccgg cttttcagaa tgcgatccca accggtaact
     9361 ggatgtatcc ggtggcaaat gtcacgctgc ctgccgggtt tgaacaattg accaaaccgg
     9421 ccaccacgct ggagttcaca ccagctgaag tggcggcaca acgtcaggca tggattagcg
     9481 aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg taagcgccgc
     9541 cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga acgcgccgca
     9601 gggtaactgg gtggcagtct ggcaggacag ctacctgtgg catgtggtgc gcttctcctt
     9661 ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc tcgcccgcgc
     9721 cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg caatgacctt
     9781 gatcctcccg gtgctagttg ctgttttcgg cattcttagc gtctatggtc gtcagggctg
     9841 gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct acggcctgca
     9901 aggcattttg ctggcgcacg tattttttaa tctgccgatg gcgagccgct tattactcca
     9961 ggcactggaa aacattcccg gcgaacaacg tcagcttgcc gcccagcttg ggatgcgcgg
    10021 ctggcatttt ttccgcttcg tcgaatggcc gtggctacgg cgacaaatcc cgccggttgc
    10081 cgcgcttatc tttatgctct gtttcgccag cttcgccacc gtgctatcgc tgggcggcgg
    10141 tccgcaggca accactatcg agctggctat ttatcaggcg ctgagttacg actacgatcc
    10201 tgcccgcgcg gcgatgctgg cgctgctcca gatggtgtgt tgccttgggc tggtgctgtt
    10261 gagtcagcga ctaagtaagg ccattgcgcc cggcaccacg ctgctgcaag gctggcgcga
    10321 tccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc tggcgctgct
    10381 gctgttgctg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc agttgccgga
    10441 agtgctggca caaccggtgc tgtggcaggc gctgtggacc tcgttgcgta ttgcgctggc
    10501 ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc gcgaactgcg
    10561 ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt tgatcctcgc
    10621 catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca ctatcggcct
    10681 gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga tcccttatgc
    10741 gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagta tgttgtgtca
    10801 gtcgctgggc attgaaggct ggtcgcgctt aaaagtggtc gagctgcgcg ccctgaaacg
    10861 tccactggcg caggcgctgg cttttgcctg cgtgctgtcg attggtgatt ttggcgtagt
    10921 ggcgttgttc ggtaacgatg atttccgtac cctgccgttt tatctctacc agcaaattgg
    10981 ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac tctgttttct
    11041 gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact gatatcacct
    11101 ggctttatca ccatttgccg atgcgtttta gcttaacggt agaacgcggc gagcaggtgg
    11161 caatcctcgg gccaagcggc gcgggtaaaa gtaccctgct gaatttgatt gccggttttc
    11221 tgacgccagc cagcggttcg ctgactatcg atggtgttga tcacaccacg acgccgccgt
    11281 cacgccgtcc ggtgtcgatg ctgtttcagg agaacaacct gttcagccat ctgacggtcg
    11341 cgcagaacat cgggctgggg ctaaatccgg ggttgaaact gaacgcggca cagcaggaga
    11401 aaatgcacgc gattgcccgt cagatgggga ttgataattt aatggcgcgg ttaccgggcg
    11461 agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggtg cgcgaacagc
    11521 cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgttacgt caggagatgc
    11581 tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gctattgatg gtgtcgcaca
    11641 gtgtggaaga tgcggcgcgg atcgccacac gctcggtggt ggtcgccgac gggcgcatcg
    11701 cctggcaggg taagactgaa gaattgttga gcggtaaggc gagtgcttcg gcaatattgg
    11761 ggattacggg ttagtatgca ctgtcagatg cggcgtgaac accttatccg acctacggac
    11821 agtgcgtttt gtaggcctga taagacgcgg tcagcgtcgc atgaggcaac ccgcacaaga
    11881 caatccccta aaccccaacc actttacgca aaatatcgat atacaccggc atcaacgggt
    11941 ggcgaattaa caccaccagc gccaccacgc cgatggcaga aatcaacggc gtcagccaca
    12001 acaaacggcc gcgcgacaaa taatgactta aacgatcggt tgctttaccg ctgcgccata
    12061 accgccagca cagccagcca ccaacccaca aaaataccgc cgtcgccagc agcaaccatt
    12121 taaactcgcc gctctgcatt ccggctggaa tatcgatcgc cgcgcccgcc agaatcccag
    12181 gcaggaagta aaacggtggc cacagcaggc agccgataat attcggcgta atgaattttg
    12241 ccaccggcag atccagcatt cccgccacca ttggcaccag cggacgcgtc gggccaacaa
    12301 agcgaccgac cagaatggtg aacatgctgt gttgatgcaa cgcatgttca gttttatcga
    12361 gtaatgcttt gtttttcttc agaaatgacc agcgatgcaa cggcttttta aaacgccagc
    12421 ccagccagaa ggagatccag tcgcccagca gacaaccgac aatccctgcc agccaagcgt
    12481 gccagaaact taactcgccg ctgccaatca gcgctcccag ccccgccatc agcaccgtac
    12541 cgggtagaat caaaccgacc agcgccagcg actccagaaa ggccaccaac accaccgcca
    12601 tcaatgaata cacggtagat tgggtaataa agtgttccag caatgcttgc ataatgtgcc
    12661 tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt
    12721 gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa
    12781 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag
    12841 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc
    12901 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct
    12961 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg
    13021 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt
    13081 acacgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac
    13141 aattgctcaa gcagattcat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg
    13201 ttaatgattt gcccaaacag gtcgctgaaa tacggctggt gcgcttcatc cgggcgaaag
    13261 aaacccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga
    13321 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacaacc gtagtgatga
    13381 atctctcctg gcgggaacag caaaatatca cccggtcggc agacaaattc tcgtccctga
    13441 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc
    13501 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc
    13561 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt
    13621 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt
    13681 cactgcgtct tttactggct cttctcgcta acccaaccgg taaccccgct tattaaaagc
    13741 attctgtaac aaagcaggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat
    13801 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca
    13861 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt
    13921 tctccatacc cgtttttttg gatggagtga aacgatggcg attgcaattg gcctcgattt
    13981 tggcagtgat tctgtgcgag ctttggcggt ggactgcgcc agcggtgaag agatcgccac
    14041 cagcgtagag tggtatcccc gttggcaaaa agggcaattt tgtgatgccc cgaataacca
    14101 gttccgtcat catccgcgtg actacattga gtcaatggaa gcggcactga aaaccgtgct
    14161 tgcagagctt agcgtcgaac agcgcgcagc tgtggtcggg attggcgttg acagtaccgg
    14221 ctcgacgccc gcaccgattg atgccgacgg taacgtgctg gcgctgcgcc cggagtttgc
    14281 cgaaaacccg aacgcgatgt tcgtattgtg gaaagaccac actgcggttg aagaagcgga
    14341 agagattacc cgtttgtgcc acgcgccggg caacgttgac tactcccgct atattggcgg
    14401 tatttattcc agcgaatggt tctgggcaaa aatcctgcat gtgactcgcc aggacagcgc
    14461 cgtggcgcaa tctgccgcat cgtggattga gctgtgcgac tgggtgccag ctctgctttc
    14521 cggtaccacc cgcccgcagg atattcgtcg cggacgttgc agcgccgggc ataaatctct
    14581 gtggcacgaa agctggggcg gcttgccgcc agccagtttc tttgatgagc tggacccgat
    14641 cctcaatcgc catttgcctt ccccgctgtt cactgacacc tggactgccg atattccggt
    14701 gggcacctta tgcccggaat gggcgcagcg tctcggcctg cctgaaagcg tggtgatttc
    14761 cggcggcgcg tttgactgcc atatgggcgc agttggcgca ggcgcacagc ctaacgcact
    14821 ggtaaaagtt atcggtactt ccacctgcga cattctgatt gccgacaaac agagcgttgg
    14881 cgagcgggca gttaaaggta tttgcggtca ggttgatggc agcgtggtgc ctggatttat
    14941 cggtctggaa gcaggccaat cggcgtttgg tgatatctac gcctggtttg gtcgcgtact
    15001 cggctggccg ctggaacagc ttgccgccca gcatccggaa ctgaaagcgc aaatcaacgc
    15061 cagccagaaa caactgcttc cggcgctgac cgaagcatgg gccaaaaatc cgtctctgga
    15121 tcacctgccg gtggtgctcg actggtttaa cggtcgtcgc acgccaaacg ctaaccaacg
    15181 cctgaaaggg gtgattaccg atcttaacct cgctaccgac actccgctgc tgttcggcgg
    15241 tttgattgct gccaccgcct ttggcgcacg cgcaatcatg gagtgcttta ccgatcaggg
    15301 gatcgccgtc aataacgtga tggcgctggg cggcatcgcg cggaaaaacc aggtcattat
    15361 gcaggcctgc tgcgacgtgc tgaatcgccc gctgcaaatt gttgcctctg accagtgctg
    15421 tgcgctcggt gcggcgattt ttgctgccgt cgccgcgaaa gtgcacgcag acatcccatc
    15481 agcccagcaa aaaatggcca gtgcggtaga gaaaaccctg caaccgcgca gcgaacaggc
    15541 acaacgcttt gaacagcttt atcgccgcta tcagcaatgg gcgatgagcg ccgaacaaca
    15601 ctatcttcca acttccgccc cggcacaggc tgcccaggcc gttgcgactc tataaggaca
    15661 cgataatggc gatttttgat aattatgaag tgtggtttgt cattggcagc cagcatctgt
    15721 atggcccgga aaccctgcgt caggtcaccc aacatgccga gcacgttgtt aatgcgctga
    15781 atacggaagc gaaactgccc tacaaactgg tgttgaaacc gctgggcacc acgccggatg
    15841 aaatcaccgc tatttgccgc gacgcgaatt acgacgatcg ttgcgctggt ctggtggtgt
    15901 ggctgcacac cttctccccg gccaaaatgt ggatcaacgg cctgaccatg ctcaacaaac
    15961 cgttgctgca attccacacc cagttcaacg cggcgctgcc gtgggacagt atcgatatgg
    16021 actttatgaa cctgaaccag actgcacatg gcggtcgcga gttcggcttc attggcgcgc
    16081 gtatgcgtca gcaacatgcc gtcgttaccg gtcactggca ggataaacaa gcccatgagc
    16141 gtatcggctc ctggatgcgt caggcggtct ctaaacagga tacccgtcat ctgaaagtct
    16201 gccgatttgg cgataacatg cgtgaagtgg cggtcaccga tggcgataaa gttgccgcac
    16261 agatcaagtt cggtttctcc gtcaatacct gggcggttgg ctatctggtg caggtggtga
    16321 actccatcag cgacggcgat gttaacgcgc tggtcgatga gtacgaaagc tgctacacca
    16381 tgacgcctgc cacacaaatc cacggcgaaa aacgacagaa cgtgctggaa gcggcgcgta
    16441 ttgagctggg gatgaagcgt ttcctggaac aaggtggctt ccacgcgttc accaccacct
    16501 ttgaagattt gcacggtctg aaacagcttc caggtctgcc cgtacagcgt ctgatgcagc
    16561 agggttacgg ctttgcgggc gaaggcgact ggaaaaccgc cgccctgctt cgcatcatga
    16621 aggtgatgtc aaccggtctg cagggcggca cctcctttat ggaggactac acctatcact
    16681 tcgagaaagg taatgacctg gtgctcggct cccatatgct ggaagtctgc ccgtcgattg
    16741 ccgtagaaga gaaaccgatc ctcgacgttc agcatctcgg tattggtggt aaggacgatc
    16801 ctgcccgact gatcttcaat acccaaaccg gtccagcgat tgtcgccagc ctgattgatc
    16861 tcggcgatcg ttaccgtctg ctggttaact gtatcgacac ggtgaaaaca ccgcactccc
    16921 tgccgaaact gccggtggcg aatgcactgt ggaaagcgca accggatctg ccaactgctt
    16981 ccgaagcgtg gatcctcgct ggtggcgcgc accataccgt cttcagccat gcgctgaacc
    17041 tcaacgatat gcgccagttc gccgagatgc acgacattga aatcacggtg attgataacg
    17101 acacccgcct gccagcgttt aaagacgcac tgcgctggaa cgaagtgtat tacggatttc
    17161 gtcgctaagt agccacatca ggtatgtaac gcctgatgcg acgctgacgc gtcttatctg
    17221 gcctacacgc tgcgattttg taggccggat aagcaaagcg catccggcat tcaacgcctg
    17281 atgcgacgct ggcgcgtctt atcaggccta cgcgctgcga ttttgtagac cggataagcg
    17341 aagcgcatcc ggcacgaagg agtcaacatg ttaaaagatc tcaaacgcca ggtattagaa
    17401 gccaacctgg cgctgccaaa acacaacctg gtcacgctca catggggcaa cgtcagcgcc
    17461 gttgatcgcg agcgcggcgt ctttgtgatc aaaccttccg gcgtcgatta cagcgtcatg
    17521 accgctgacg atatggtcgt ggttagcatc gaaaccggtg aagtggttga aggtacgaaa
    17581 aagccctcct ccgacacgcc aactcaccgg ctgctctatc aggcattccc ctccattggc
    17641 ggcattgtgc atacgcactc gcgccacgcc accatctggg cgcaggcggg tcagtcgatt
    17701 ccagcaaccg gcaccaccca cgccgactat ttctacggca ccattccctg cacccgcaaa
    17761 atgaccgacg cagaaatcaa cggcgaatat gagtgggaaa ccggtaacgt catcgtagaa
    17821 acctttgaaa aacagggtat cgatgcagcg caaatgcccg gcgttctggt ccattcccac
    17881 ggcccgtttg catggggcaa aaatgccgaa gatgcggtgc ataacgccat cgtgctggaa
    17941 gaggtcgctt atatggggat attctgccgt cagttagcgc cgcagttacc ggatatgcag
    18001 caaacgctgc tggataaaca ctatctgcgt aagcatggcg cgaaggcata ttacgggcag
    18061 taatgactgt ataaaaccac agccaatcaa acgaaaccag gctatactca agcctggttt
    18121 tttgatggat tttcagcgtg gcgcaggcag gttttatctt aacccgacac tggcgggaca
    18181 ccccgcaagg gacagaagtc tccttctggc tggcgacgga caacgggccg ttgcaggtta
    18241 cgcttgcacc gcaagagtcc gtggcgttta ttcacgccga tcaggttccc cgcgctcagc
    18301 atattttgca gggtgaacaa ggctttcgtc tgacaccgct ggcgttaaag gattttcacc
    18361 gccagccggt gtatggcctt tactgtcgcg cccatcgcca attgatgaat tacgaaaagc
    18421 gtctgcgtga aggtggcgtt accgtctacg aggccgatgt gcgcccgcca gaacgctatc
    18481 tgatggagcg gtttatcacc tcaccggtgt gggtcgaggg tgatatgcac aatggcacta
    18541 tcgttaatgc ccgtctgaaa ccgcatcccg actatcgtcc gccgctcaag tgggtttcta
    18601 tagatattga aaccacccgc cacggtgagc tgtactgcat cggcctggaa ggctgcgggc
    18661 agcgcatcgt ttatatgctg gggccggaga atggcgacgc ctcctcgctt gatttcgaac
    18721 tggaatacgt cgccagccgc ccgctgctgc tggaaaaact caacgcctgg tttgcaaacc
    18781 acgatcccga tgtgatcatc ggctggaacg tggtgcagtt cgatctgcga atgctgcaaa
    18841 aacatgccga gcgttaccgt cttccgctgc gtcttgggcg cgataatagc gagctggagt
    18901 ggcgcgagca cggctttaaa aacggcgtct tttttgccca ggctaaaggt cggctaatta
    18961 tcgacggtat cgaggcgctg aaatccgcgt tctggaattt ctcttcattc tcgctggaaa
    19021 ctgtcgctca ggagctatta ggcgaaggaa aatctatcga taacccgtgg gatcgaatgg
    19081 acgaaattga ccgccgtttc gccgaagata aacctgcgct ggcaacttat aacctgaaag
    19141 attgcgagct ggtgacgcag atcttccaca aaactgaaat catgccattt ttactcgaac
    19201 gagcaacggt gaacggcctg ccggtggatc ggcacggcgg ttcggtggca gcgtttggtc
    19261 atctctattt tccgcgaatg catcgcgctg gttatgtcgc gcctaatctc ggcgaagtgc
    19321 cgccgcacgc cagccctggc ggctacgtga tggattcacg gccagggctt tatgattcag
    19381 tgctggtgct ggactataaa agcctgtacc cgtcgatcat ccgcaccttt ctgattgatc
    19441 ccgtcgggct ggttgaaggc atggcgcaac ctgatccaga gcacagtacc gaaggttttc
    19501 tcgatgcctg gttctcacgg gagaaacatt gcctgccgga gattgtgact aacatctggc
    19561 acgggcgcga taaagccaaa cgtcagggta ataagccgct ttcgcaggcg ctgaagatca
    19621 ttatgaatgc cttttatggc gtgctcggca ccaccgcctg ccgcttcttc gatccgcgcc
    19681 tggcatcgtc gatcaccatg cgtggtcatc agatcatgcg gcagaccaaa gcattgattg
    19741 aagcgcaggg ctacgacatt atctacggag ataccgactc gacttttgtc tggctaaaag
    19801 gtgcacattc ggaagaagag gcggcgaaaa tcggtcgtgc actggtgcag cacgttaacg
    19861 cctggtgggc ggaaacgctg caaaaacaac ggctgaccag cgcattagaa ctggagtatg
    19921 aaactcattt ctgccgtttt cttatgccaa ccattcgcgg agctgatacc ggcagtaaaa
    19981 agcgctacgc cgggctgatt caggagggcg acaagcagcg gatggtgttt aaagggctgg
    20041 aaaccgtgcg caccgactgg acgccgctgg cccagcagtt tcagcaggag ctgtacctgc
    20101 gtatcttccg caacgagcca tatcaggagt acgtccgcca aaccatcgac aaactgatgg
    20161 cgggtgaact ggatgcgcgg ctggtttacc gtaaacgcct tcgccgtccg ctgagcgaat
    20221 atcagcgtaa tgttccgcct catgtacgcg ccgctcgcct tgccgatgaa gaaaatcaaa
    20281 agcgcggtcg ccccttgcaa tatcagaatc gcggcaccat taagtacgta tggaccacca
    20341 acggcccgga gccgctggac taccaacgtt caccactgga ttacgaacac tatctgaccc
    20401 gccagctaca acccgtggcg gagggaatac tcccttttat tgaggataat tttgctacac
    20461 ttatgaccgg gcaacttggg ctattttgag caaaaaaaag agttcgccag ataccatttt
    20521 gatgcgtgac gaatgctttg ccatccagta ccatagcgcc ctttccattc ctggacctga
    20581 ataacaccac tacctcataa gcacggtagc gggtggttat tgcctgcaat taaagatata
    20641 gagccgaaca catatgcctt ttacacttgg tcaacgctgg atcagcgata cagaaagcga
    20701 attgggactt ggaaccgttg tcgcggtgga tgcgcgaact gtcactttac ttttcccatc
    20761 tactggtgaa aaccgtctgt acgcacgcag tgattccccc gtgacccgcg tgatgttcaa
    20821 ccctggtgat accattacca gccatgacgg ctggcagatg caagtcgaag aagtaaaaga
    20881 agaaaatggc ttgctgacct atatcggtac tcgcctggat actgaagagt ccggcgtagc
    20941 cctgcgtgaa gttttccttg atagcaaact ggtgttcagc aaaccgcagg accgtctgtt
    21001 tgccgggcag attgaccgta tggaccgctt tgcgctgcgt tatcgcgcgc gtaagtattc
    21061 cagcgaacag ttccgtatgc cgtacagcgg cctgcgcggt cagcgtacca gcctgatccc
    21121 gcatcagctc aacatcgctc atgatgttgg tcgccgccac gcgccgcgcg tcctgctggc
    21181 tgacgaagtg ggtttaggga aaaccattga agccgggatg atcctgcatc agcaactgct
    21241 ctctggcgct gctgaacgtg tgctgattat cgtcccggaa accttacagc atcagtggct
    21301 ggtagaaatg ctgcgccgtt tcaacctgcg ctttgctctg tttgatgatg agcgttatgc
    21361 cgaagctcag cacgatgctt acaacccgtt tgacaccgaa cagctggtga tttgctcgct
    21421 ggattttgcc cgtcgtagca aacagcgctt ggaacatctc tgtgaagccg aatgggacct
    21481 gctggtggtc gatgaagcgc atcacctggt gtggagcgaa gatgcgccaa gccgtgaata
    21541 tcaggccatt gaacaactgg cagagcacgt gccgggcgtt ctgctgctga ccgcgacccc
    21601 ggaacagctg gggatggaaa gccacttcgc ccgtctgcgt ctgctggacc cgaaccgttt
    21661 ccacgatttt gcgcagttcg ttgaagagca gaaaaattat cgtccggttg cggacgccgt
    21721 tgccatgctg ctggcaggta acaaactgag caatgacgaa ctgaacatgc tcggcgagat
    21781 gatcggcgag caggatatcg agccgctgtt gcaggcagca aacagcgaca gcgaagatgc
    21841 ccagagcgcc cgtcaggagc tggtttcgat gctgatggat cgccacggca ccagccgcgt
    21901 gctgttccgt aacacgcgta acggtgtgaa aggattcccg aaacgcgagc tgcacaccat
    21961 taagctgccg ctaccgacgc agtatcagac ggctattaaa gtctccggca ttatgggcgc
    22021 acgtaaaagt gcggaagatc gtgctcgcga tatgctctac ccggagcgta tttatcagga
    22081 atttgaaggt gataacgcca cctggtggaa cttcgatccg cgcgttgagt ggctgatggg
    22141 ctacctgacc agccatcgct ctcagaaagt gctggtgatc tgcgccaaag ctgccactgc
    22201 gctgcaactg gagcaggtac tgcgcgaacg tgaaggtatt cgcgctgcgg tgttccacga
    22261 aggtatgtcg attatcgaac gtgaccgcgc tgccgcctgg tttgccgaag aagacaccgg
    22321 cgcacaggta ctgctgtgct cagaaatcgg ttctgaagga cgtaactttc agttcgccag
    22381 tcacatggtg atgtttgacc tgccattcaa cccggatcta ctggagcagc gtattggtcg
    22441 tctggatcgt atcggccagg cgcacgatat tcagatccat gtgccttatc tggagaaaac
    22501 cgctcagtcg gtgctggtgc gctggtatca cgaaggtctg gatgcatttg agcacacctg
    22561 cccgaccgga cgcactattt acgatagcgt atacaacgat ctgattaact atctggcttc
    22621 accggatcaa accgaaggct ttgacgatct gatcaaaagc tgccgcgagc aacatgaagc
    22681 gctgaaagca cagctggaac agggtcgtga ccgcctgctg gaaatccact ccaacggtgg
    22741 cgaaaaagcc caggcactgg cagaaagcat tgaagagcag gatgacgata ccaacctgat
    22801 cgccttcgcc atgaacctgt tcgatattat cggtatcaat caggacgatc gcggcgacaa
    22861 catgatcgtg ctgacgccgt ccgatcatat gctggtgccg gacttccctg gcctgtcgga
    22921 agatggcatc accatcacct ttgatcgtga agtggcgctg gcgcgtgaag atgcacagtt
    22981 tattacctgg gagcatccgc tgatccgcaa cggtctggat ctgatccttt ctggcgatac
    23041 cggtagcagc acgatttcac tgttaaaaaa caaagcgttg ccggtaggta cgctgttggt
    23101 ggaactgatt tatgtggttg aagcccaggc tccgaagcag ttgcagctca accgcttcct
    23161 gccaccgacg ccggtacgta tgctgctgga taaaaacggc aacaacctgg cggcgcaggt
    23221 agagtttgaa acctttaacc gccagcttaa cgcggttaac cgtcacaccg gcagcaaact
    23281 ggttaacgcc gtgcagcagg atgttcacgc tatccttcaa ctgggtgaag cgcagatcga
    23341 gaaatctgcc cgtgcattga ttgatgcagc gcgtaacgaa gccgacgaaa aactgtctgc
    23401 cgagctgtct cgtctggaag ctctgcgtgc agtgaacccg aacattcgtg acgacgaact
    23461 gaccgccatt gagagcaacc gtcagcaggt aatggaaagc ctggatcagg caggttggcg
    23521 tctggatgcc ctgcgtttga tcgttgtaac gcatcagtaa cggagccgaa aatggggatg
    23581 gaaaactaca atccaccgca ggaaccctgg ttggttatcc tgtatcagga tgaccatatt
    23641 atggtggtca acaagccgag cggtttgttg tcagtgccgg gtcgtctgga agagcacaaa
    23701 gacagcgtga tgacgcgcat tcagcgtgat tatccgcagg cagaatcggt gcatcgtctg
    23761 gatatggcta ccagcggcgt gattgtagtg gcgctgacca aagccgcgga gcgggagtta
    23821 aaacgccagt tccgcgagcg cgagccgaaa aagcagtatg tggcccgcgt ctgggggcat
    23881 ccatcccccg cagaaggtct ggtggatctg ccgctgattt gcgactggcc aaaccgcccg
    23941 aaacagaaag tctgttacga aacgggtaaa cctgcgcaga cggaatatga agtggtggag
    24001 tatgcggcgg ataacacggc aagagtggtg ttaaaaccga ttaccgggcg ttcgcatcaa
    24061 ttgcgtgtgc atatgctggc gctggggcat ccgattctcg gcgatcgttt ttatgcatca
    24121 ccagaagcga gagcgatggc accgcgtttg ttgctacatg cagagatgtt gacgattacc
    24181 catccggcgt atggcaatag tatgacgttt aaagcgccag cggattttta atcgtcacgc
    24241 tggcgcagtt cggtaggcgg ataaggcgtt cacgccgcat ccggcaattg tgcgccgatt
    24301 gcctgatgcg acgcgggccg cgtcttatca ggcctacagt ttaaaactta tttaaaccct
    24361 ttctgctgct ttatcagctc atatgcctgc tgaatttcct gcgctttctg cttcgccatc
    24421 tccatcatct caggcggcaa acctttcgcc accagcttat cgggatggtg ttcactcatc
    24481 agcttacggt aggcacgttt gatggtggtc gcatcatccg tcggcttcac gcccagcaca
    24541 ttacaggcat cttccagcgt cgggccacgc tgcgcttgct gccagttacc accgccagat
    24601 tgctgctgat aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa aaactggtca
    24661 aactgggcgc gggagatccc taattcttca gcaatgacat acagcaccgc ccgttcattc
    24721 gggtgcagtg aaccatcagc aaacgccgcc tgaatctgga tctccagaaa catacgaatt
    24781 aagtcaaaac gaccaaagca gacactgcga aactggcgca tcttttcgcg cagcgggtaa
    24841 ttatctgatt ttcccacccg gaacgcgttt tgcgccgcag tacgggaagc gccatgaagg
    24901 ttcattcggt ccatcaactg gctggcgata tgaatatcag cctccgtgac gcgacctttg
    24961 gatttggtta aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca
    25021 cgctggttgg cgaaccacgc cattttacgg ctacgggctt tatcaaacat atggccaatt
    25081 aacaggccta acactacgcc ccaaaagccg ccgcccatca gtaaggccac ggccacgcca
    25141 atgatttttc cccaatactg catatattcc ccaaatcgac acacggatat cagggctatc
    25201 tcccacaata taaaggtgct tttaccgttt tccggctgcg gtcaattgtg ggacatcgcc
    25261 tataatttgc attatcatac ctgtcattca atgccgtgcc taacaccact gacgctattc
    25321 ggacaggatt aacactagcg tagagatgac gagtacgtta gtctctgacc gtttgtcacg
    25381 cgcaacgtta ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta tccccactct
    25441 cctggccacc atgattgcca ccgcccttta tagtcaacag ggactggcag ccgacctcgc
    25501 ctcacagtgc atgttgggcg tgccaagcta tgaccgtcct ctggtacagg gcgataccaa
    25561 tgacttaccc gtgactatca atgctgacca cgcgaaaggg gactacccgg atgacgccgt
    25621 gtttactggc agcgtggata tcatgcaggg taacagccgt ctgcaggccg acgaagtgca
    25681 gctccatcaa aaagaggcac caggacaacc ggagccggta cgtaccgttg atgcgctcgg
    25741 taatgtccat tacgacgata accaggtgat cctcaaaggg ccgaaaggct gggcgaatct
    25801 gaacaccaaa gataccaacg tctgggaagg tgattaccag atggtgggtc gccagggtcg
    25861 cggtaaagcg gacctgatga aacaacgtgg cgaaaaccgc tataccattc tggataacgg
    25921 tagctttacc tcctgtctgc cgggttctga cacctggagc gtggtaggta gcgaaattat
    25981 tcatgaccgc gaagaacaag ttgctgagat ctggaacgcc cgctttaagg tgggtccggt
    26041 accgatcttt tatagcccct atttgcagtt gccggtgggt gacaaacgtc gctctggttt
    26101 cttgatcccg aacgccaagt acaccaccac caactacttt gagttctacc tgccatatta
    26161 ctggaacatc gcgccaaata tggatgccac catcacgccg cattatatgc atcgtcgtgg
    26221 caacatcatg tgggagaacg aattccgcta cctctcccag gcgggcgctg gcttgatgga
    26281 actggactat ctgccttcag ataaagtcta tgaagatgaa cacccgaacg atgacagttc
    26341 acgtcgttgg ttattctact ggaaccactc cggggtcatg gatcaggtgt ggcgtttcaa
    26401 cgtcgactac accaaggtca gcgatcctag ctacttcaat gatttcgata acaagtacgg
    26461 ttccagtact gacggctacg caacgcaaaa attcagcgtt ggctatgcgg tgcaaaactt
    26521 caatgccacc gtttcaacca agcagttcca ggttttcagc gaacagaaca ccagtagcta
    26581 ctcggcagag ccgcagttag acgttaatta ctaccagaat gatgttggtc cgtttgatac
    26641 gcgtatttac ggccaggcag tgcactttgt taacaccaga gacgacatgc ctgaagcaac
    26701 ccgtgttcac ctggaaccga ccatcaattt gccgctctct aataactggg gcagcatcaa
    26761 taccgaagcg aagtttctgg caacccatta tcagcaaacc aatcttgact ggtataactc
    26821 cagaaacacg accaagctgg acgaatccgt taaccgcgta atgccgcaat tcaaagttga
    26881 cggcaaaatg gtctttgaac gcgatatgga aatgctggct ccgggttata cccaaacgct
    26941 ggaaccgcgc gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca tctataacta
    27001 cgactcgtct ctgctgcaat ctgactactc tggcctgttc cgggaccgga cttacggcgg
    27061 tcttgaccgt attgcctccg ctaaccaagt gacgaccggt gtcacatctc gcatatatga
    27121 tgatgctgcc gttgaacgtt ttaatatttc cgttggtcaa atctactatt tcacggagtc
    27181 tcgcactggc gatgacaaca taacatggga gaatgacgac aaaacgggct cactggtgtg
    27241 ggcaggcgac acctactggc gtatctccga gcgttgggga ttgcgtggcg ggattcagta
    27301 cgatacacgt ctggataacg tagcgaccag taactccagc attgaatacc gtcgggatga
    27361 agaccgtctg gtacagctga attaccatta cgccagcccg gaatatattc aggctacgct
    27421 gcctaagtac tattccactg ctgagcaata taagaatggt atttcgcagg taggtgctgt
    27481 cgccagcagg ccaattgccg atcgttggtc cattgttggg gcctactact acgacaccaa
    27541 tgcgaacaag caagccgact ctatgttagg tgtgcaatac agctcctgct gctatgcaat
    27601 tcgcgtcggt tacgagcgga agctgaacgg ttgggataac gataaacaac atgcggtata
    27661 tgacaacgca atcggcttta acatcgaact tcgcggcctg agctccaact acggtctggg
    27721 tacgcaagag atgctgcgtt cgaacattct gccgtatcaa aacactttgt gatttgttga
    27781 tttaccacgt aatccgcagt gcggttaatt gaaatggaaa aagtatgaag aactggaaaa
    27841 cgctgcttct cggtatcgcc atgatcgcga ataccagttt cgctgccccc caggtagtcg
    27901 ataaagtcgc agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt gatggattaa
    27961 tgcagtcggt aaaactgaac gctgcgcagg caaggcagca gcttcctgat gacgcgacgc
    28021 tgcgccacca aatcatggaa cgtttgatca tggatcaaat catcctgcag atggggcaga
    28081 aaatgggagt gaaaatctcc gatgagcagc tggatcaggc gattgctaac atcgcgaaac
    28141 agaacaacat gacgctggat cagatgcgca gccgtctggc ttacgatggt ctgaactaca
    28201 acacttatcg taaccagatc cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg
    28261 tgcgtcgtcg catcaccatc ctgccacagg aagtcgaatc cctggcgcag caggtgggta
    28321 accaaaacga cgccagcact gagctgaacc tgagccacat cctgatcccg ctgccggaaa
    28381 acccgacctc tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt gtcgatcagg
    28441 cgcgtaacgg cgctgatttc ggtaagctgg cgattgctca ttctgccgac cagcaggcgc
    28501 tgaacggcgg ccagatgggg tggggccgta ttcaggagtt gccggggatc ttcgcccagg
    28561 cattaagcac cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc gttggcttcc
    28621 atattctgaa agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc
    28681 atgctcgcca tattctgctg aaaccgtcgc cgatcatgac tgacgaacag gcccgcgtga
    28741 aactggagca aattgctgct gatatcaaga gtggtaaaac gacttttgct gccgcagcga
    28801 aagagttctc gcaggatcca ggttctgcta accagggcgg tgatctcggc tgggctacac
    28861 cagatatttt cgatccagcc ttccgtgacg ccctgacccg cctgaacaaa ggtcaaatga
    28921 gtgcaccggt tcactcttca ttcggctggc atttaatcga actgctggat acccgtaatg
    28981 tcgataaaac cgacgctgcg cagaaagatc gtgcataccg catgctgatg aaccgtaagt
    29041 tctcggaaga agcagcaagc tggatgcagg aacaacgtgc cagcgcctac gttaaaatcc
    29101 tgagcaacta atggttaaaa cccaacgtgt tgtgatcact cccggcgagc ccgccgggat
    29161 tggcccggac ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg
    29221 tgccgatgcc actctcctta ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg
    29281 cccttattcc cccaactccc ctgcacaacc gcaaactacg ggcacattaa cgctacttcc
    29341 tgtcgcgcta cgtgaatctg tcactgcggg gcagttagcg attgaaaatg ggcattacgt
    29401 ggtggagacg ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat
    29461 cacaggcccc gtgcataaag gcgtcattaa cgacgcaagc attccgttta ccggtcatac
    29521 cgagtttttc gaagagcgtt cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga
    29581 acttcgcgtg gcgctggcaa cgacgcattt accgctgcgc gatatcgcag atgctatcac
    29641 ccctgcactt ttgcacgaag tgattgctat tttgcatcac gatttgcgta caaaatttgg
    29701 tattgccgaa ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca
    29761 tatgggtacg gaagagatag acaccattat tccggtgctc gacgagctgc gggcgcaggg
    29821 gatgaaactc aacgggccgc tgcctgccga taccctgttt cagccgaaat atctcgataa
    29881 cgccgacgcc gtgctggcga tgtaccacga tcagggtctt cccgtgctaa aataccaggg
    29941 cttcgggcgc ggtgtgaaca ttacgctggg cctgcccttt attcgcacat cagtggacca
    30001 cggcaccgcg cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt ttattacggc
    30061 gcttaatctc gccatcaaaa tgattgttaa cacccaatga ataatcgagt ccaccagggc
    30121 cacttagccc gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt gatcgacagc
    30181 attgtttctg ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg ccccggtctg
    30241 gcggcattga ccgagccggt cggcgaacgt ctggaccagc tgacggtcat cgaacttgac
    30301 cgcgatctgg cggcacgtct gcaaacgcat ccattcttag gcccgaaact gacgatttat
    30361 cagcaggatg cgatgacctt taactttggc gaactggccg agaaaatggg tcagccgctg
    30421 cgtgttttcg gcaacctgcc ttataacatc tccacgccgt tgatgttcca tctgtttagc
    30481 tatactgatg ccattgccga catgcacttt atgttgcaaa aagaggtggt gaatcgtctg
    30541 gttgcaggac cgaacagcaa agcgtatggt cgattaagcg tcatggcgca atactattgc
    30601 aacgtgatcc cggtactgga agttccgccg tcagccttta caccaccacc caaagtggat
    30661 tccgccgtcg tgcgcctggt ccctcatgca acgatgcctc acccggttaa agatattcgc
    30721 gtgttgagcc gcatcaccac cgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc
    30781 ctcggcaacc tgtttagcgt cgaggtgtta accggaatgg ggatcgaccc ggcgatgcga
    30841 gcggaaaata tctctgtcgc gcaatattgc cagatggcga actatctggc ggagaacgcg
    30901 cctttgcagg agagttaacg atgatcaatt cgccccgagt gtgtattcag gttcaaagcg
    30961 tctacattga ggcacaatct tcacctgata atgaacgtta cgtttttgct tataccgtaa
    31021 ccatacgcaa tctggggcga gcgccagtgc agttgttggg gcgttactgg ctgatcacca
    31081 atggcaatgg ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc cagccactta
    31141 tcgcgcctgg cgaagagtac cagtacacca gcggtgcaat cattgaaacc ccgctgggca
    31201 ccatgcaggg tcactacgaa atgatcgatg aaaatggcgt ccctttcagc atcgacattc
    31261 ccgtattccg actcgccgtt cccacactca ttcattaaaa gaatatggcg acatacctta
    31321 ttggcgacgt tcatggttgt tacgatgaac tgatcgcatt gctgcataaa gtagaattta
    31381 cccccgggaa agataccctc tggctgacgg gcgatctggt cgcgcgcggc ccgggttcgc
    31441 tggatgttct gcgctatgtg aaatccttag gcgacagcgt acgtctggtg ctgggcaatc
    31501 acgatctgca tctgctggcg gtatttgccg ggatcagccg caataaaccg aaagatcgcc
    31561 tgacaccgct gctggaagcg ccggatgccg acgagctgct taactggctg cgtcgccagc
    31621 ctctgctgca aatcgacgaa gagaaaaagc tggtgatggc ccacgcaggg atcacgccgc
    31681 agtgggatct gcagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg ctgtcgagtg
    31741 actcctatcc cttctttctt gacgctatgt acggcgatat gccaaataac tggtcaccgg
    31801 aattgcgggg gctgggaaga ctgcgtttta tcaccaacgc ctttacccgt atgcgttttt
    31861 gcttcccgaa cggtcaactg gatatgtaca gcaaagaatc gccggaagaa gcccctgccc
    31921 cactgaaacc gtggtttgcg attcctggcc ctgtcgctga agaatacagc atcgcctttg
    31981 gtcactgggc atcgctggaa ggcaaaggta cgccggaagg tatttacgcg ctggataccg
    32041 gctgctgctg gggtgggtca ttaacctgcc tgcgctggga agataaacag tattttgtcc
    32101 agccgtcgaa ccggcataag gatttgggcg aagcggcggc gtcttaaaca cagcctgata
    32161 caggaaggcc ggataagacg cgaccggcgt cgcatccggc actagccgta aattctatac
    32221 aaacttaccg ccgctccaga atctcaaagc aatagctgtg agagttctgc gcatcggcgt
    32281 cgtggaattc gctgaatacc gattcccagt catccggctc gtaatccggg aaatgggtgt
    32341 cgccttccac ttctgcgtcg atatgcgtca gatacagttt ttgcgctttt ggcaggaact
    32401 gctcataaac gcgaccgccg ccaatcacca tgatttctgg tacgtcacca cacgccgcaa
    32461 tggcttcatc caccgatttc acccacgtta cgcgatcgtc cgtacccggt tgactgctga
    32521 ggataatatt tttgcgtcct ggcaacggac gaccgattga ttcccaagta tggcggccca
    32581 taatcacggg tttatttaag gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc
    32641 acggcatggc gttttccatg ccgataacgc gatctaccgc taacgccgca atcagactga
    32701 tcattgagat ttcccgataa aaaaattgtc gccactatac gtaaagcgta aaccgtcgtc
    32761 gactggtgcg aggatgatgt tgaggaaaat tttatattct gctggcgagt ccacgctctc
    32821 cccctggact cgccgcatta caatgaaaca aaaacaaaca gttagctgta aagtgtgatt
    32881 tacgtcactc tttattaaga tgagggtttc gtttccggtt catccgccat gttgccggta
    32941 tgtttacctt cttctgttcc ctgccagcca tgtcgttgaa ttaatgacag atgttcgcgg
    33001 tcctcggtga tgatctcact taacatcgcg ctggtgcgtt tatagaccgc cgcgcgggct
    33061 ttggtgtcat tctcaaccat tgccatctct tccaccatct gaatattaaa gcggcggaac
    33121 acatcggcac gttctcgcgc ttcatacggc cccagaccta aactttccag cgccagacgc
    33181 ccggttttca gcgcaccttc aaaggtttca cgctccggct tttcaacgcc tgcctgacgc
    33241 aaacgaatgt agtgatcgac atcgcgggcg cgggcaataa tctgcaaatg cgggaaatgt
    33301 tctttcacca tctccgtcag ttgcaggttg gtttgcggat cgtcaatagc gttaatcagc
    33361 acttccgctt tcgccgctcc ggcagattcc agtaaatcca tccgcgtggc atcgccataa
    33421 aacactttca taccaaattt acgcagcgtt tcgatatggt ccggatcgtg atcgagcacc
    33481 accattttca ccccgctgga gagcagtaaa cgtccggtaa tctgtccaaa acgaccgaat
    33541 ccggcgataa tcacgcgcgg ctgttcttca tcgatctcat cggcttcacg cgcctcctcc
    33601 gtagaagatt gctcaaggcg attgaggatc accagcagaa tcggcgttgc tgccatcgac
    33661 agcgccaccg ccagggtcag cgatttcgcc cactccggct ccagcacatt cgccatctgc
    33721 gccgcaccaa ataccacaaa ggcaaactca ctgccctgcc ctaacaacac cgcaaaccag
    33781 cgacgctgtt tatttggcac ctgcaacggt cgggcaatca gccacagcat ggcgattttg
    33841 atgatgagga aaccgagcag caaaatgaca atgcgcaatg ggttttcaat cagcgtgcca
    33901 aagtctatcg acatgccaac accgatgaaa aacagcccca acagcaaacc tttaaacggt
    33961 tcgatatcgc tttccaacgc atgacggtat tccgagcttg ccagcagtac gcccgccaga
    34021 aacgcgccca tcgccatcga caagccgacc tcttccagca gcaaaccaaa gccaaacaca
    34081 aggaataacg ccacggcact aaacgcttcc cgcaagccag agcgggcaac aaaacgcagc
    34141 gccggacgcg taacatagcg ccccagcaat accaccagta ccagcgcacc cgccactttt
    34201 aacgccgaga gagcaaatgc gcccatcgtc gtcgaggcac tgctcgccgc cagtagcggg
    34261 atcatcgcca ccagcgggat tgccgcgata tcctggaaca gcagcactgc aaaggcactg
    34321 cggcccattt gcgtcaccat cagattgcgt tcattcatcg cctgcatggc aatcgccgtt
    34381 gatgagagcg ccagcgtcat accgatcaat tccgcgacct gccagcgcag cccaagtaac
    34441 atacagaaca gccccagcag gccaccgcaa atcaccatct gcaatgcgcc accgccgaac
    34501 actgccgcac gcagcttcca cagcctttgt ggatcgagtt cgaggccgat aataaacagc
    34561 atcagcacca ccccaatctc ggcaaagtgc agaatagatt cggcatcggt caccagtcgc
    34621 agcccccacg ggccaataat gcagccggcg atcaggtagc caagtaccga tcccagacca
    34681 agacgtaccg caatgggtac aatcagcgcc gccgatccga ggtagatcag cgcctgaacc
    34741 agcgtatgac tatccatgat gggcctcctg ccattccagc agacgttgct tatagtggcg
    34801 cgcctgccct tcgagggttt cgtcgtcaca aataaaggtg cagtgcatgg caaacggtgg
    34861 cagccagttc agcccgcagt agattgccgt cgcctgtagc ggctgcgaca gcacatcaaa
    34921 gcccggatgc gcaccaattt caaaatggct ttccccgccg ccggtcgtca ccgcccacag
    34981 caaatgtttg ccatgcagcg ccgtgccgcc gtgaccgtaa gcccagccgt gcgacaaaac
    35041 tttatcgatc caaagtttaa gaagcggagg aatgctgtac cactgcatcg ggtgctgcca
    35101 gacgatcaga tcggcgcgag agagcgcctc ctgctcggcg gcaatatcga tattgaagtc
    35161 aggatagagt tgataaagag agcgaatttc gacgccttcc agcgtccttg cctgttcaag
    35221 catccgttta ttcgcatggg aatgatgcgg atacggatgc gcataaatga tgaggatcat
    35281 agttattagc ccgcgtgttt acgcctttgt tttcccacaa agtgtagtca tatattcacg
    35341 cggcttatag tcaataatat tgattcagaa aagagagaaa tctgaacaaa taatcccatt
    35401 ctctgcccgc ggatcgtcat attgctggtc tgcgccagtg acattcctcg agtctccggg
    35461 gcaaacgcta cggaaatcaa caagccaaac agcgagatac ccgcccccat tagcatcgtg
    35521 ttactgatac cgtaattatt gataaatatc ggcagtgccc aggtcgaaac aatggtgcca
    35581 atacgactta aggacataat cacgcccacg gcagaggcgc ggatatccgt cgggaagagt
    35641 tcattaggat agagccactg caaattaccc ggcccgccag agaaaaaggc atacaccgca
    35701 aaggccatca ctaccagcca gatccccata tccgggatta gccccaaaac cgccaacgcc
    35761 agcgtcatca tggcaaagct gccaatcaac aatggacgcc gtccggcagt gtttagccac
    35821 agcatcggcg gaatacagcc gagcataaag aacaggctaa tcaccacatt ccctagtgcc
    35881 gcgtttttgc caacccccaa tcccaacgaa ccaacgattt gcgggccaaa ggtgtaaatg
    35941 gcgaacattg ggatcacctg gcaggtccag atggcggcaa caaacagaac aaaaggaaaa
    36001 tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg gctgctcttc atcgaaagcc
    36061 accggttcgc caaacagttt tatcatcatc tcttcgcact cttttactcg ccctttgcgt
    36121 aataaccagc ggggagattc tggcagttcg aatcgaccaa tcaaaatcaa caaacaaggg
    36181 atcgccgcgc tacccagcat ccagcgccaa ccaccttcca catcataaag ccagtagccg
    36241 accagatcgg cgcaggtcgc gccgacatac cacatcgcgg caataaagct gatggaaaac
    36301 gcccgctgac gggtactgga gaactcggtg atcatcgaag tggcgatggg ataatctgca
    36361 ccgatgacaa tgccgatgag tacccgcatc accaacagtt cgacggggga tgaaacacac
    36421 atcgtcgcca ccgatatcac gccgatggcg atgatatcaa tgaggaacat tttgcgccgt
    36481 ccgactttat cggagatata gccaaacagc gatgtgccaa cgaacagccc ggcgagcgtt
    36541 cccgcgccca gcaagccaat ccagtcagcg tccagtttca gcgccggagt cagttgctcc
    36601 agcgccacgc caatcattac cagtacataa ccatccagaa acggaccacc gcttccccac
    36661 agcaaaatgc ggcggtgaat agaggagaat ttgagatcgt caaagtttct ggacggttgc
    36721 atgggttatt cctgtttttt tgcaatgaga gtcagtacgg cgttacgtat caaaccaaca
    36781 tcagccgtaa cggaactcca caccaaaggt gccgcgcggg tattcccact gttccagcgc
    36841 cgaccccagc cccagaatgc gacaggtgcc gcactccaga catccggcgt aatcgaagcg
    36901 cacactgccg tcatcctgct tcttgtacag acctgcgggg cacgctttca ccagcagctc
    36961 cagcacctgt ttatcagcat cggccttcac aacaatgtgc ggatgctctt catcgacatt
    37021 gaatttattg acgcccagtt tgacgtccac attgacggga gaagtcataa aacggtcact
    37081 cctttcatgc catccttgat cagattgatg aagcccactt tcttgccgtg gcggaggatt
    37141 ttcttgcgca tcagttccgg cgcactgccg tcaatggtga acaggtcacg cgccacgccc
    37201 accgccagtt ccgggttgcc gctaaacatg cgtgggttat caaggaacgc cggtagtttc
    37261 tggtacatgc gcatatcgcg cagcgggcca ctctccagat gctgacgata ttccgcgagt
    37321 ttttgcttac tgaaatcgtc gcttttcatc gctgaaagca cggtttttgc tgcagcttcc
    37381 ccggcggcaa tcgccagatc cataccgcga atggtaaaac cgaggttcat acacattccg
    37441 gcggcatcac cggcaatcaa tacgccgtca ccgaccagct ccggcagcat gttgatgcct
    37501 gcttccggca ctacgtgagc ggaatattcc accagcttgc cgcccgcgat cagcggtgca
    37561 acggccggat gctgtttgaa atcttccagc atttgcggca ccgatttttt cgcgtcatgc
    37621 agatgatgca gaccacaaac cagccccagc gacagggtat tttcattggt ataaaggaag
    37681 ccgccgccca tcaggccatc ggtgggtgat cccgcaaaca gacaagccgc gccctgatta
    37741 ccctgcaact gaaaacggtc ttcgattacc gacttcggta actcgatcag ttccttcacg
    37801 ccaaccgcca catccgtcgg tttaacgcgt tttgccatcc ccaacttttc ggcaaggatg
    37861 gagttcaccc catcagcaag gagcaccgtt ttcgcttcaa tcacatcacc atcggcttct
    37921 acaccgacga ctttgccatc gcgctgtacg aggttatcga cgcggatccc ggtaattaac
    37981 tgcgcgcccg cttcttcggc ctgctccatc aaccaggcat caaatttact gcgcaaaacg
    38041 gagtaagaac gctgggatgg cgaggtttcg tcaccgttgc agtagtccat agtcatcgct
    38101 gacttttccg tcataaacgc gagtttttca tgggtgatca ggcgttctac gggggcggaa
    38161 tcggcgaaac ctggaataat gtgttccaga ctgtgggcat agagacgccc gccagtaacg
    38221 ttcttggcac ctgcggaatt gccacgctcg ataactaaca cttgcgcccc ttcgcgggcg
    38281 agcaccagtg cggcaaccga accggcaagc cctgcaccga cgatgatggc gtcaaagata
    38341 tcttcggaca taagagctcc ctgtcagcgg cagagccgta atgcagccct gccagagtgg
    38401 atcaacgcgc taaagctgcg gtcagtgccg gaaggatctt cacggcatcg ccaacaatgc
    38461 cgtaatccgc gtactggaag atcggcgcat ttttatcttt attgatggcg aaaatggttt
    38521 gcgacgcgtt agcgccaacc atgtgctgga tctacccgga gatccccacc gccaggtaca
    38581 gttcaggttt cagcatcagg ttggagatac cgacatagcg ttcgtgctcc atccattttt
    38641 cgttttctgc caccggacga gaacaggcca actccgcacc tatcgccttg caaagctgtt
    38701 ctgccagcgc aatgttctct ttgctgccaa tgccgcgacc gacgctgacc accagacggg
    38761 ctttgtcgag atcgacgctg ttgctctggc gcgcctgggt tgccgtgcgg gtaatcgcca
    38821 cagccggagc ctgccactcc acggtgtgcg tttcgccagt gcgtgacgcg tctggctgag
    38881 ccgcatcgaa cgtgccgctg ctgatggtca gtaccgcata cggcgtggca atgcgttctt
    38941 cgccaatcgc cagaccgccg taaaccatgt gtttcactgt tgctttaccg tcctgtacgc
    39001 tgacggtgct ggcatcgtta gacaccgccg ctttaaggcg ataacccagt tttgccgcca
    39061 gtaatttgcc gcgacgggtg tttggcaaca gcaccagacc gtcagcgccg tgctggcgaa
    39121 tagtgtcagc catgacaccg gcgtaatctt cgatcatccg atcgtccggt ttgccgttta
    39181 atttccagac atgattagcg ccgagctgga ttgcctgtgc gccgtcggca tcattgagga
    39241 caaaggcatt gatttgatta gctaaagcct gcgcaccgtt catcagttcc ggcagacgag
    39301 agggggtatc gctgaatacc cagacttgag aaaacgtgtt catagcatcc cctgtaatta
    39361 aatgactttg cgaagatttt cagcaaatgc ggcgatctgt tcttcgccgt cgccttcaat
    39421 cacgatgcgc tgacgctcgc gctgtttcgg cgcggcaacc tgttgttcag accaggcgtc
    39481 cacggcgtta aaaccaatat ccgccgccga ccatacctgg actggctttt tcgccgcgcc
    39541 gagaatggct ttcatcgaag gaatttgtgg ggagttgata tcagtggaaa cagcaacaac
    39601 cgcaggcagc ggaatgctta aggtttcggt ttcatcttcc agttcgcgct caacggtgag
    39661 ggtatctgcc gtcagggaga taattttgct gacgccgtta actgccggaa tattgaggat
    39721 ttcgcccacc agcagaccaa cctgctgggc ataaaggtcg gaagaaccat cgccacagag
    39781 gatcagatca aagcctgctt tctgggcggc tgcagccagt gcgctcgccg tttgttgcgg
    39841 cagtgcctgc tcgaactggt catcaatcac cacaatcagt tcatccgggc cgcgcgatag
    39901 cacatcttta cgccctttgg cgttggtcag ggctttaccg cccacactta aggctgtcac
    39961 ctgcgcctct gctgcctgtt gctttagctg gcaagccgct tcaatagcgt tgagatcgta
    40021 ttggcttatt ttggcatcgg ctttgctgaa gtctaatgaa ccatcagcat tattgaccgc
    40081 aatatcctgt tcatcaggca cgcacttata gcaagtaata atcttcattg catctccaga
    40141 aatcatgaag gaaacgtcgt tcatatcggt ggtgttaatt acagatatca cgccctttaa
    40201 tttggaaata aaactttaat caccaatatt gaaagtcaga tgctattttt ttacccacaa
    40261 aagaatataa caaactgatt aataaccaaa attaacgaaa aacgccctct gttcactcga
    40321 aacaaaaatg tgataccaat cacagaatac agcttattga atacccatta tgagttagcc
    40381 attaa
//
LOCUS       DAZSNO010000023        39989 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZSNO010000023 DAZSNO010000000
VERSION     DAZSNO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39989
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.023"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(24..278)
                     /locus_tag="ACHWN2_001133"
     CDS             complement(24..278)
                     /locus_tag="ACHWN2_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR8930757.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="ACHWN2_001134"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="ACHWN2_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR8930758.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="ACHWN2_001135"
     CDS             1228..2694
                     /locus_tag="ACHWN2_001135"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR8930759.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHWN2_001136"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHWN2_001136"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR8930760.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHWN2_001137"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHWN2_001137"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR8930761.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="ACHWN2_001138"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="ACHWN2_001138"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR8930762.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="ACHWN2_001139"
     CDS             5709..7265
                     /locus_tag="ACHWN2_001139"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR8930763.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="ACHWN2_001140"
     CDS             7347..9161
                     /locus_tag="ACHWN2_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR8930764.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="ACHWN2_001141"
     CDS             9162..10256
                     /locus_tag="ACHWN2_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR8930765.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="ACHWN2_001142"
     CDS             10256..11281
                     /locus_tag="ACHWN2_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8930766.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="ACHWN2_001143"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="ACHWN2_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR8930767.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="ACHWN2_001144"
     CDS             complement(12876..13220)
                     /locus_tag="ACHWN2_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR8930768.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="ACHWN2_001145"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="ACHWN2_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFR8930769.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="ACHWN2_001146"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="ACHWN2_001146"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR8930770.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="ACHWN2_001147"
     CDS             15616..17376
                     /locus_tag="ACHWN2_001147"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR8930771.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="ACHWN2_001148"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="ACHWN2_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR8930772.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="ACHWN2_001149"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="ACHWN2_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR8930773.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="ACHWN2_001150"
     CDS             19113..19340
                     /locus_tag="ACHWN2_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR8930774.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="yejM"
                     /locus_tag="ACHWN2_001151"
     CDS             19360..21120
                     /gene="yejM"
                     /locus_tag="ACHWN2_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR8930775.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="ACHWN2_001152"
     tRNA            21195..21271
                     /locus_tag="ACHWN2_001152"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="ACHWN2_001153"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="ACHWN2_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFR8930776.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="ACHWN2_001154"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="ACHWN2_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR8930777.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="ACHWN2_001155"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="ACHWN2_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR8930778.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="ACHWN2_001156"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="ACHWN2_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR8930779.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="ACHWN2_001157"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="ACHWN2_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR8930780.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="ACHWN2_001158"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="ACHWN2_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR8930781.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="ACHWN2_001159"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="ACHWN2_001159"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR8930782.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="ACHWN2_001160"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="ACHWN2_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR8930783.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="ACHWN2_001161"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="ACHWN2_001161"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR8930784.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31429)
                     /gene="ccmA"
                     /locus_tag="ACHWN2_001162"
     CDS             complement(30806..31429)
                     /gene="ccmA"
                     /locus_tag="ACHWN2_001162"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR8930785.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="ACHWN2_001163"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="ACHWN2_001163"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR8930786.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="ACHWN2_001164"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="ACHWN2_001164"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR8930787.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="ACHWN2_001165"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="ACHWN2_001165"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR8930788.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="ACHWN2_001166"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="ACHWN2_001166"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR8930789.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="ACHWN2_001167"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="ACHWN2_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR8930790.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="ACHWN2_001168"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="ACHWN2_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR8930791.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="ACHWN2_001169"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="ACHWN2_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR8930792.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /gene="yojO"
                     /locus_tag="ACHWN2_001170"
     CDS             37391..37555
                     /gene="yojO"
                     /locus_tag="ACHWN2_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR8930793.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37669..38178
                     /gene="eco"
                     /locus_tag="ACHWN2_001171"
     CDS             37669..38178
                     /gene="eco"
                     /locus_tag="ACHWN2_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR8930794.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(38300..39924)
                     /gene="mqo"
                     /locus_tag="ACHWN2_001172"
                     /pseudo
     CDS             complement(38300..39924)
                     /gene="mqo"
                     /locus_tag="ACHWN2_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
BASE COUNT         9750 a        10810 c         9875 g         9554 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttaacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gagatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaaca
//
LOCUS       DAZSNO010000024        36685 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZSNO010000024 DAZSNO010000000
VERSION     DAZSNO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36685
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.024"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            85..441
                     /locus_tag="ACHWN2_001173"
     CDS             85..441
                     /locus_tag="ACHWN2_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR8930795.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            445..1572
                     /gene="dapE"
                     /locus_tag="ACHWN2_001174"
     CDS             445..1572
                     /gene="dapE"
                     /locus_tag="ACHWN2_001174"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR8930796.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1600..1800
                     /locus_tag="ACHWN2_001175"
     CDS             1600..1800
                     /locus_tag="ACHWN2_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR8930797.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1910..2608)
                     /gene="ypfH"
                     /locus_tag="ACHWN2_001176"
     CDS             complement(1910..2608)
                     /gene="ypfH"
                     /locus_tag="ACHWN2_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR8930798.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(2682..4697)
                     /gene="tmcA"
                     /locus_tag="ACHWN2_001177"
     CDS             complement(2682..4697)
                     /gene="tmcA"
                     /locus_tag="ACHWN2_001177"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR8930799.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(4712..5575)
                     /locus_tag="ACHWN2_001178"
     CDS             complement(4712..5575)
                     /locus_tag="ACHWN2_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR8930800.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5743..6456)
                     /gene="purC"
                     /locus_tag="ACHWN2_001179"
     CDS             complement(5743..6456)
                     /gene="purC"
                     /locus_tag="ACHWN2_001179"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR8930801.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(6669..7703)
                     /gene="bamC"
                     /locus_tag="ACHWN2_001180"
     CDS             complement(6669..7703)
                     /gene="bamC"
                     /locus_tag="ACHWN2_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR8930802.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(7720..8598)
                     /gene="dapA"
                     /locus_tag="ACHWN2_001181"
     CDS             complement(7720..8598)
                     /gene="dapA"
                     /locus_tag="ACHWN2_001181"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR8930803.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            8744..9316
                     /locus_tag="ACHWN2_001182"
     CDS             8744..9316
                     /locus_tag="ACHWN2_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR8930804.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            9316..9786
                     /gene="bcp"
                     /locus_tag="ACHWN2_001183"
     CDS             9316..9786
                     /gene="bcp"
                     /locus_tag="ACHWN2_001183"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR8930805.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            10039..10656
                     /gene="hyfA"
                     /locus_tag="ACHWN2_001184"
     CDS             10039..10656
                     /gene="hyfA"
                     /locus_tag="ACHWN2_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR8930806.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            10656..12673
                     /gene="hyfB"
                     /locus_tag="ACHWN2_001185"
                     /pseudo
     CDS             10656..12673
                     /gene="hyfB"
                     /locus_tag="ACHWN2_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            12684..13631
                     /gene="hyfC"
                     /locus_tag="ACHWN2_001186"
     CDS             12684..13631
                     /gene="hyfC"
                     /locus_tag="ACHWN2_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR8930807.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQNYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            13648..15087
                     /gene="hyfD"
                     /locus_tag="ACHWN2_001187"
     CDS             13648..15087
                     /gene="hyfD"
                     /locus_tag="ACHWN2_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFR8930808.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVDFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            15099..15749
                     /gene="hyfE"
                     /locus_tag="ACHWN2_001188"
     CDS             15099..15749
                     /gene="hyfE"
                     /locus_tag="ACHWN2_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR8930809.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            15754..17340
                     /gene="hyfF"
                     /locus_tag="ACHWN2_001189"
     CDS             15754..17340
                     /gene="hyfF"
                     /locus_tag="ACHWN2_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR8930810.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            17330..19044
                     /gene="hyfG"
                     /locus_tag="ACHWN2_001190"
                     /pseudo
     CDS             17330..19044
                     /gene="hyfG"
                     /locus_tag="ACHWN2_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            19054..19599
                     /gene="hyfH"
                     /locus_tag="ACHWN2_001191"
     CDS             19054..19599
                     /gene="hyfH"
                     /locus_tag="ACHWN2_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR8930811.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            19596..20354
                     /gene="hyfI"
                     /locus_tag="ACHWN2_001192"
     CDS             19596..20354
                     /gene="hyfI"
                     /locus_tag="ACHWN2_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR8930812.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            20347..20760
                     /gene="hyfJ"
                     /locus_tag="ACHWN2_001193"
     CDS             20347..20760
                     /gene="hyfJ"
                     /locus_tag="ACHWN2_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR8930813.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            20790..22802
                     /gene="hyfR"
                     /locus_tag="ACHWN2_001194"
                     /pseudo
     CDS             20790..22802
                     /gene="hyfR"
                     /locus_tag="ACHWN2_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            22824..23672
                     /gene="focB"
                     /locus_tag="ACHWN2_001195"
                     /pseudo
     CDS             22824..23672
                     /gene="focB"
                     /locus_tag="ACHWN2_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(23710..24771)
                     /locus_tag="ACHWN2_001196"
     CDS             complement(23710..24771)
                     /locus_tag="ACHWN2_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR8930814.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            24984..26447
                     /gene="bepA"
                     /locus_tag="ACHWN2_001197"
     CDS             24984..26447
                     /gene="bepA"
                     /locus_tag="ACHWN2_001197"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR8930815.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            26468..26827
                     /gene="arsC"
                     /locus_tag="ACHWN2_001198"
     CDS             26468..26827
                     /gene="arsC"
                     /locus_tag="ACHWN2_001198"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR8930816.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(26960..27661)
                     /locus_tag="ACHWN2_001199"
     CDS             complement(26960..27661)
                     /locus_tag="ACHWN2_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR8930817.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(27756..29045)
                     /gene="uraA"
                     /locus_tag="ACHWN2_001200"
     CDS             complement(27756..29045)
                     /gene="uraA"
                     /locus_tag="ACHWN2_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR8930818.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(29131..29757)
                     /gene="upp"
                     /locus_tag="ACHWN2_001201"
     CDS             complement(29131..29757)
                     /gene="upp"
                     /locus_tag="ACHWN2_001201"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR8930819.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            30081..31118
                     /gene="purM"
                     /locus_tag="ACHWN2_001202"
     CDS             30081..31118
                     /gene="purM"
                     /locus_tag="ACHWN2_001202"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR8930820.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            31118..31756
                     /gene="purN"
                     /locus_tag="ACHWN2_001203"
     CDS             31118..31756
                     /gene="purN"
                     /locus_tag="ACHWN2_001203"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR8930821.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            31928..33994
                     /gene="ppk1"
                     /locus_tag="ACHWN2_001204"
     CDS             31928..33994
                     /gene="ppk1"
                     /locus_tag="ACHWN2_001204"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR8930822.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            33999..35540
                     /gene="ppx"
                     /locus_tag="ACHWN2_001205"
     CDS             33999..35540
                     /gene="ppx"
                     /locus_tag="ACHWN2_001205"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR8930823.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(35579..>36685)
                     /gene="pdeF"
                     /locus_tag="ACHWN2_001206"
     CDS             complement(35579..>36685)
                     /gene="pdeF"
                     /locus_tag="ACHWN2_001206"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR8930824.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFISNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         8497 a         9701 c         9534 g         8953 t
ORIGIN      
        1 tatattttct gataatcatt tgcaggcaaa atgttttcac ccttaaatga gtatttattc
       61 tcataaatcg aaaaaggatt cattatggtt acactttacg gcatcaaaaa ttgtgacacc
      121 attaaaaagg ctcgccgttg gctggaagcc aataacatcg actatcgttt tcatgattac
      181 cgcgtcgatg ggctggacag cgaattattg aacggtttta tcaacgaatt aggctgggaa
      241 gcgttactca acacccgtgg tacaacctgg cgtaaactgg acgaaactac ccgcaataaa
      301 atcaccgatg cggcctctgc ggcggcatta atgactgaaa tgcctgcaat tatcaaacgt
      361 ccattgctct gcgcgcctgg taagcctatg ctgctgggtt tcagtgattc cagttatcag
      421 caatttttcc atgaggtgta gtctatgtcg tgcccggtta ttgagctgac acaacagctt
      481 attcgccgcc cttccctgag tcctgatgac gcaggatgtc aggctttgtt gattgaacgt
      541 ttgcaggcga tcggttttac cgttgaacgc atggattttg ccgatacgca gaatttttgg
      601 gcatggcgtg ggcaaggtga aacgttagcc tttgccgggc ataccgacgt ggtgccgcct
      661 ggcgacgccg atcgttggat caatccgccg tttgaaccca ccattcgtga cggcatgtta
      721 ttcgggcgcg gtgcggcaga tatgaaaggc tcgctggcgg cgatggtggt agcagcagaa
      781 cgttttgtcg cacaacatcc caaccatacg gggcgactgg catttctgat cacttctgat
      841 gaagaagcca gtgcccacaa cggtacggta aaagtcgtcg aggcgttaat ggcacgtaat
      901 gagcgtctcg attactgcct ggtcggcgaa ccgtcgagta tcgaagtggt aggtgatgtg
      961 gtgaaaaatg gtcgtcgcgg atcgttaacc tgcaacctca ccattcatgg cgttcagggg
     1021 catgttgcct acccacatct ggctgacaat ccggtacatc gcgcagcacc tttccttaat
     1081 gaattagtgg ctattgagtg ggatcagggc aatgaattct tcccggcgac cagtatgcag
     1141 attgccaata ttcaggcggg aacgggcagt aacaatgtta ttccgggtga actgtttgtg
     1201 cagtttaact tccgcttcag caccgaactg actgatgaga tgatcaaagc gcaggtactt
     1261 gccctgcttg aaaaacatca actgcgctat acggtggatt ggtggctttc cgggcagcca
     1321 tttttgaccg cgcgcggtaa actggtggat gcggtcgtta acgcggttga gcactataat
     1381 gaaattaaac cgcagctact gaccacaggc ggaacgtccg acgggcgctt tattgctcgc
     1441 atgggggcgc aggtggtgga actcgggccg gtcaatgcca ctattcataa aattaatgaa
     1501 tgtgtgaacg ctgccgacct gcagctactt gcccgtatgt atcaacgtat catggaacag
     1561 ctcgtcgcgt gatgagtggt tctgcaagag gaaataagca tggactggct ggctaaatat
     1621 tggtggattc tggtgattgt ctttttggta ggcgtcctgc tgaacgtgat taaagatctc
     1681 aagcgcgtcg accataagaa atttctcgcc aacaagccgg agcttccccc gcatcgtgat
     1741 ttcaacgata agtgggacga tgacgacgac tggccgaaaa aggatcaacc gaagaagtaa
     1801 gctgagtgct caaccgtagg ccggataagg cgttaacgcc gcatccggca atggtgaacg
     1861 atccctgatg cgacgctcac gcgtcttatc aggccaggta atctgtcttt tacatcattt
     1921 caatcacatc atcatcgcca ggcttaccgc cacttagcgc ttggtcgaaa taatgtttcg
     1981 gaatggtata acgcaagtga tccagggcaa attgcatact gcgattgtcg atcgcatggc
     2041 ctaaatcctc cacgatatcc agcgtcacgt caccaccggc actgattaac gcttcctgcg
     2101 ccgcaactgc atgcgccaga tcgataaccg gatcttcacc accatgaatc aagtgaattg
     2161 tcgtggctgt agacgcggtc tccggcaggc tcgcatagcg tccgttaaag gcaatgacgc
     2221 gcgaagcaag gcccggttcg gctttaatgc tctccagcgc cataatcgcg ccttgtgaga
     2281 aaccaattag cgccgtggcg ttagccccta ctccgctctg tttctgccag tagcgtaccg
     2341 tttcaataaa cgtcggcatg atggcatcca cacgcgcctg gcgattatct tccgtaatac
     2401 cctgcactga aaaccactga cgccccgccg gattaccgct cggctccgcg ccaccgacac
     2461 tcaccaccag cgcatcagga aacagcggtg caaaccagct gcctatttcc cccattgcca
     2521 ccgggttatc cccgacaccg tggaaaagca gtaacaactg ttgtgcaggt ttatccgggc
     2581 tttgaacaac aaaatggtca tgtttcatgg cgaactcctt aactgatgtc attaatttta
     2641 cgccgctgac tcacaatgac catgggagaa aattgaagga gtcagtgaaa aaattgccat
     2701 tgcgttatgc gatcgcgcag acgctcagtg cgaacctcat ttagtgcgaa cagcgcctgc
     2761 gcggcctctt cccgctggcg aaccagtaac atcttgcggc ctgaaagttt aagtgtggta
     2821 cataactgcg cgtcactggc gtttttctgt aaacgcccac gcagcgccgg taatgccagt
     2881 tcactggttt gtagcagacg caataagcaa cctaacgatg ttaatagcgg acgatgagcg
     2941 aaagcaaaac cggccagttc aagccagtcg tcgtcagaaa ggatggcatc gtttagtgga
     3001 tcaacaggaa gcatttcgcc attccactgc gcgagagctt gcgcatcgcg acgtaaacgg
     3061 taatgctcac gttcagccag ctgtttaccc gcatcactca tcggtaacag cgccatcgcc
     3121 gtatagcaac cgctgctggc ttcccgatga ttacccatcc gcaccagcac aaaaccgcag
     3181 cgttgccaga aacgccataa ctccccagtg taaccaaaac tcaccgaaag atagtcgagg
     3241 tcatgcgtat attgcaaagc tccagcaata agttgtcgcc ctgtgccttc ccgctgacga
     3301 gccggatgaa ctgctatccg gctaacccgc cttccgcgca atgtcgccgc cagtggattg
     3361 ctgccgtgcg ccgccagcga ctgggccacc agattacccc gcgggcgacg aaaacctgcc
     3421 cataccgcct gactgagttc ttgagataat ccgccctcat ccaccagcca caacgccccg
     3481 gcaatctcgt tttcgccagc cgcctgtaaa aaatgttgcc ctggtgcatc catcatccgg
     3541 cgtaaatcca gcggcgaggt ccggtagtgc gcaccagaca ataactgata aacctttaac
     3601 ggcgtttctg gctcgcttcg ccataacgtt tgttcaaatg cggaaatgac gatattgcct
     3661 tgtggtgtat gggtgaagtt ttcatcgtta aaaaccagtg cctcactaac cattttttcc
     3721 agcgggcatc cctgtgccca gcggatcggc tgttgcagtt caaaacggtg taaatgcgga
     3781 aagcgagcgc aaaatttcag caaaaaacca cgtccggtac cttcgtagcc ctgcaccgta
     3841 gtggttaaca acgttcgagg aaaacgcgat accagttgat gcaacaacgg cgcaggtatg
     3901 gctgcggctt cgtcgaccac cagccagtcg gcttgctcat cgctggctaa caaggcatcc
     3961 ggtgcaataa agcgaaactt ctcgcccgca aattgtgcca gtacatccgt tgccgctttt
     4021 gcgggcgcgg tgacaatcgc actgcccgca atacgagaaa tgagttgccc tgccaacgcc
     4081 gacttaccgc gcccacgcgc agccgttacc gctgccacgc ccggcggcat ggtcagtagc
     4141 tgctgtaaga gttgctgttg ttctggttgt ggtgcgccgg tcgcggggtg ccagtcagta
     4201 cggggagtaa aatgcgccaa cgagaacggc tggttttgcc gccagaggat agcgtcgtta
     4261 tccgccgtaa gtacgcgttt gagatgctgg acaaaatgcg gcgtcgcaat agggtcaggg
     4321 caatcactcc agcgcagcga gtcggcatca ggttggtttt cccactcttc ccatacaggg
     4381 agtaacaaaa ccagccagct tcccgctttc aacgttccgc taagtgctgc aaaggcagcg
     4441 gcatcaaagc cgtggcgggc gtcgaatacc gcatgccgga actcgcgccc aagtaaagtt
     4501 tgtagcgccg agggtgaaca gtggttttca gcatctagct gcggcgaaat ccacagccag
     4561 tcgccaggta aggcatcacg caacttaaga gcatgatcaa aacaccaacc ctcttccccg
     4621 ctcaacacca gcaagcggca gatcccttca cgtttcattt gcgctgttaa tgtgtaaagc
     4681 gcagtcagtt cagccatccc tgccccggaa attaaatgct tttaccaaaa gtattgcatt
     4741 gtgccggatc gccgctgtcg aaaccacgtt taaaccagct gtagcgttgc tgagaagtgc
     4801 catgtgtgaa actgtctggt actactcgcc cctgactttg ctgttgtaaa cggtcatcgc
     4861 cgatggcctg cgccgcattc agcgcctctt ccagatcgcc agtttccaga acgccttgct
     4921 gctgcatact atgcccccag acaccggcaa aacagtcggc ctggagttcc atacgcacag
     4981 ataagcggtt tacttccgcc tgcgtcgcgt tttgttgcag ttgacgaact ttcggctcga
     5041 tgcctaacag tttctgcaca tgatgaccga cttcatgggc gataacgtac ccctgggcaa
     5101 aatcgccatc cgcgccaagt ttgtctttca tgtcatcata gaaggagaga tcgatataaa
     5161 ctgtgccatc cgccgggcaa tagaacggcc ccataatgga ctggcccgcc ccgcagccgg
     5221 tgcgcgtcat tccacggtac atgaccagct tcggttgctg ataggtctta cccatctttt
     5281 cgaactgttg tccccaggtg tcttccgtgg ttgcctgaat caccgaggtg aattttgcgg
     5341 cttcgtcttc attagggcta attgaccgcg ttgattgttg ttgagaaacc ggctgcccgg
     5401 tcatcaaccc ggttaaatca acgccatagt agcctgcaac cagcacgact atcagtaaaa
     5461 tcagcccgcc tttaccgctc ggcagacgga aaccgggacc gcccattgac ggaccgccag
     5521 agctgttgcg cctgtcttca acattgtcac tttcacgtcg cccttgccaa cgcatagata
     5581 cctcaacaat atattcatta tagaaatgat cgtaggtggt tcagaggaag attaccacag
     5641 gaaagcggga tgaaatggcg gaggaatgag cgttcaatgc atatattccg agagcggttg
     5701 tcttgccgtg ccagctgcac ggcaagatga tgaacagaaa aatcagtcca gctgtacacc
     5761 caggcggcgg gcgacggctt cataggcttc gatcaggcca ccgaggctct ggcggaaacg
     5821 gtctttgtcc attttctcca gtgtttcttt gtcccacagg cggctgccgt ccggggagaa
     5881 ctcatcgccc agtaccactt cacctttgta cagaccaaac tccagcttga agtcgaccag
     5941 aatcagacca gcatcatcga acagtttttt cagcacgtcg ttcgctttgt aggtcagctc
     6001 tttcatacgc gccaggttct ctttgctcac ccagccaaag gtttcgcagt aagattcgtt
     6061 gaccatcgga tcgtgcatgg catcgttttt caggaacaga tcgaacagcg gcgggttcag
     6121 ctcaatacct tcttcgattc caagacgttt cactagagag ccagcagcac ggttacgcac
     6181 gacacactca accggcacca tatccagctt tttcaccaga cattcggtat cggagagcag
     6241 acgctccatt tgagtcggga tacccgcttc agccagtttg ctcataatga agtagttgaa
     6301 cttgttgttc accatacctt tgcgatcaaa ctgctcaatg cgcgcgccat cccctgctga
     6361 cgtatcattg cggaattcga gcaccaacag gtccgggttt tccgtgctgt atacggtttt
     6421 cgctttacca cgatacaact cagcttgctt ttgcatcttt atcactcctg ggtgtgaatt
     6481 aacgttttaa aatcttttgc tgtctggtgt gccggatgtt ttgtcggatg cggcgtgaac
     6541 gccttatccg aactacgtcc gacaaacaaa tttcgtgcga attaccgcta aaatcgcgtt
     6601 ttcctgccga cgcacacgtt tgcgtatcat atcagaaaaa agggccggat gattccagcc
     6661 ctgtattttt acttgctaaa cgcagcctgg aagacagcta ccagcgcgtc gttctgactc
     6721 tgagtcagag tatgaccttt cggatcgatg aactgtaggc tgctgcggtt atctaaatcg
     6781 ccaacctgca gtttatagtc accggatgcc aggcctggat cgctcgcgcc cagttcctgc
     6841 cagtcgctgt cagacagcgg cttataagtt acggccatgt tgccctgcga acgggtgctg
     6901 tcggtcactt tcatgcccac tttttccagc gccgctggca gacgttgcca aaccacattg
     6961 aacggcccgc gtacgaccag cattggtaaa ccggtgtcat cagctgcact ttgtacgtcc
     7021 atagtggtgg aggcacgatt ttgcgcagcg ttcgcggcgt cagtggcaga tttatccaga
     7081 ccggcggaaa taacgttcat catctccgtg ctgtaacgct gcatggaagc cgcgtctgca
     7141 accggtttgc ccgcctgttc caggttcagc agtttaaccg taaccgcctg ctgataaccc
     7201 tgcggcttaa cagagatttg ataacgacca cgatactgct cgtcttcgtc cagacggttc
     7261 cattgtaccc aatcggtggt cagtgtctga ccagcatcat cacgttgggt gatggtgtag
     7321 tttttcgcct gcagcacgct aaccacctgc ggccacagag tattgccacg accattttcc
     7381 accagcaatg aagcggtatc gcccgtgaac tgggtacgcg cgccagaaac cagtgccaac
     7441 ggctgggctg gtggacgaat gtccagcgcc ttaccgacag caccactacc gttggtcacc
     7501 gggattgcat aatcaccgga ggtcaccggc aaaatcattc cagccggggc atgaagctcc
     7561 gcaagcggtg ccgcttccag gtaggcttca tcaccactga cctgacgctt atagcgtgag
     7621 tcagaactac aggcagcgag taataaaaca agcgaaacac ccgcaacctt tgccaggcgc
     7681 gacttttgaa cagagtaagc catcaaatct ccctaaactt tacagcaaac cggcatgctt
     7741 aagcgccgct ctgaccgtct cacgaccact gtcggtgatt ggtgtcattg gcaggcgcag
     7801 cgtatcggtc gccacaagac ccagttcctt acatgcccat ttcaccggga ttggattggg
     7861 ttcgacaaat agtttgttgt gtaatggcat cagacgctga ttaataacgc gtgcctcggc
     7921 aaaatgccct tctgctgcca gtttgcacat ctgggccata tcacgcgctg cgacgttagc
     7981 cgtaacggaa ataactccat gaccgcccaa ttgcatgaag tccagcgcgc tcgcatcatc
     8041 gccgctcagc agaacaaaat catctgaaac cagctctttg atctggttta cacgcgttaa
     8101 gttccctgtt gcctctttga ttccgataat attttttact ttcgccagac ggcccaccgt
     8161 ttccgggagc agatcgcagc cagtacggga cggcacatta tacagaattt gcggcaggtc
     8221 agtatgctca gcgatggctt tgaaatgctg atacaaacct tcttgcgacg gacgattgta
     8281 gtaaggggtt accgtcaggc agccgacgat accactgtca ttgaagcgct gcgtcaggct
     8341 aatggcttcc gcagtagcgt tagcgccggt cccggcaatt accggaatgc gcccatcagc
     8401 cagatccagc gtcatcatca ccacatcagc atgttcgtca tgatttaagg tagcggactc
     8461 gccagtggtg ccaacagaaa cgatcgccga agtaccgctg gcgacatgat aatcaatcag
     8521 ttttttcaag ctagcccgac agacattacc tttttcatcc atcggagtaa caatcgcgac
     8581 aatacttccc gtgaacatgg gccatcctct gtgcaaacaa gtgtctcaat ggtacgtttg
     8641 gtatggcatt aaaagcaagc agacagaacc gttctgattg ttgtatgcat gattttttta
     8701 tgctttcctt aagaacaact caccccttaa aggaataacc agtttgacac tgtcatcgca
     8761 acattatctg gtgatcactg cgttgggtgc cgatcgccct ggaattgtga acaccatcac
     8821 ccgtcatgtc agtagttgcg gctgtaatat tgaagacagt cgcctggcga tgctgggaga
     8881 agagttcacg tttatcatgc tgctttccgg ttcatggaat gccatcactc tgattgaatc
     8941 aacgttaccg ttgaaaggtg ccgaactgga tcttttaatc gtgatgaagc gcacgacggc
     9001 gcgtccgcgt ccgccaatgc cagcatctgt ctgggttcag gtcgatgttg cagactcccc
     9061 gcatttaatt gaacgcttca cagcactttt cgacgcgcat catatgaaca ttgcggagct
     9121 ggtgtcgcgc acacaacctg ctgaaaatga acgggctgcg cagttgcata ttcagataac
     9181 cgcccacagc cccgcatctg cggacgcagc aaatattgag caagcgttca aagccctatg
     9241 tacagaactc aatgcacaag gcagtattaa cgtcgtcaat tattcccaac atgatgaaca
     9301 ggatggagtt aagtaatgaa tccactgaaa gccggtgata tcgcaccgaa atttagcttg
     9361 ccggatcaag acggagaaca agttaatttg accgacttcc agggacagcg tgttctggtt
     9421 tatttctacc cgaaagccat gacccccggc tgtaccgtac aggcctgcgg cttacgcgat
     9481 aacatggatg agttgaaaaa agcgggcgtt gatgtgctgg gtatcagcac cgataaaccc
     9541 gaaaaactct cccgttttgc ggaaaaagag ctgcttaact ttacgctcct gtctgatgag
     9601 gaccaccagg tgtgcgaaca attcggcgtc tggggtgaaa agtccttcat gggcaaaacc
     9661 tacgatggta ttcatcgcat cagcttcctg attgacgctg atggcaaaat cgaacatgtc
     9721 tttgacgatt tcaaaaccag caatcaccac gacgttgtgc tgaactggct gaaagaacac
     9781 gcctgattac tttgctccat tccgtgctgg ctgcgcttgc ggccagcata cctcacttct
     9841 cgtgatcaag atcacattct cgctttcccc tgcgacacgg gtgtcgaatc cattttttgc
     9901 tgaacgttaa tgaccatcat ttttgtaccg ttcagaatcc agttaataca taacttattg
     9961 aatagattga gttaatcaga atggcatcct ttatacaata tgaaatgcaa tgtttcatat
    10021 cattttcaag gagccgacat gaaccgcttt gtggtggccg aaccactgtg gtgtacagga
    10081 tgtaatacct gtctcgctgc ctgttcggac gtgcataaaa cgcaaggttt acagcaacac
    10141 ccgcgcctgg ccctggcgaa gacgtcaaca atcactgccc ctgtcgtgtg tcatcactgt
    10201 gaggaagccc cttgcctgca ggtctgcccg gtcaatgcca tctctcagag ggatgatgcg
    10261 atccaactca acgaaagcct ctgtattggc tgcaagcttt gcgccgtggt ctgcccattt
    10321 ggcgcaatca gcgcttcagg aagccgtccg gtgaatgccc atgcgcaata tgtttttcag
    10381 gctgaaggct cactcaaaga cggcgaagaa aacgtgccgc cccaacatgc gttgctgcgc
    10441 tgggaaccgg gtgtccagac cgtcgcggtg aaatgcgacc tgtgtgattt cttgccagaa
    10501 ggtccggcct gcgttcgcgc ttgcccgaat caggcgttac ggctgatcac cgacgatagc
    10561 ctgcaacgtc agatgaaaga aaaacagcgc cttgccgcaa gctggtttgc caatggcggg
    10621 gagtctcccc tttccctcac tcaggagcaa cactaatgga tgccctgcaa ttattaacct
    10681 ggtcgctgat cctctatctg tttgccagtc tggcttcgct gtttttactc ggtctggaca
    10741 gactggctat taagctttcc ggcatcacat cgctggtggg cggcgtgatt ggcatcatca
    10801 gcggaattac gcaattacat gctggtgtaa ctttagtcgc ccattttgcc accccttttg
    10861 actttgccga tttaaccctg cgaatggata gcctctcggc atttatggtg ctagttatct
    10921 ccttgctggt ggtggtttgt tcgctctatt cattgactta tatgcgcgaa tacgagggca
    10981 aaggcgcggc ggcgatgggc ttctttatga atcttttcat cgcatcgatg gttgccctgc
    11041 tggtgatgga caacgctttt tggttcatcg tgctgtttga aatgatgtcg ctgtcttcct
    11101 ggtttctggt cattgccagg caggataaaa cgtcgatcaa cgctggcatg ctctactttt
    11161 ttatcgccca cgccggatcg gtgctgatta tgatcgcctt cttgctgatg gggcgcgaaa
    11221 gcggcagcct cgattttgcc agtttcgcac gctttcactt tctccggggc tggcgtcggc
    11281 ggtgtttctg ctggcctttt tcggttttgg cgcgaaagcc gggatgatgc cgttgcacag
    11341 ctggttgccg cgcgctcacc ctgccgcacc atcgcacgct tcggcgttga tgtctggcgt
    11401 aatggtcaaa atcggtattt tcggcatcct gaaagtagcg atggatctgc tggcgcaaac
    11461 gggtttgcct ctgtggtggg gcattctggt gatggcgatc ggcgcaatct ccgcgctcct
    11521 gggcgtgcta tatgcgctgg cggaacagga tatcaaacgg ctgctggcct ggagtaccgt
    11581 cgaaaacgtc ggcattattt tgctggcagt cggtgtggcg atggacggtc tgtcactgca
    11641 cgacccgctg ctcaccgtgg ttggactgct cggcgcactg tttcatctgc tcaaccatgc
    11701 gctgttcaaa gggctgctat ttctcggcgc ggacgcgatt atttcgcgtt tgcataccca
    11761 cgacatggaa aaaatggggg cactggcgaa acggatgccg tggacagccg cagcatgcct
    11821 gattggttgc ctcgcgatat cagccattcc tccgctgaat ggttttatca gcgaatggta
    11881 cacctggcag tcgctgttct cactaagtcg tgtggaagcc gtagcgctac aacttgcggg
    11941 tcctattgct atggtaatgc tggcagtcac tggtgggctg gcagtaatgt gcttcgtcaa
    12001 aatgtacggt attactttct gtggtgcgcc gcgcagtaca cacgctgaag aggcacagga
    12061 agtgccaaat acgatgatcg tcgccatgct actgctcgcg gcactctgcg tattaattgc
    12121 gcttagtgcc agttggctgg caccgaagat aatgcatatt gcccatgcgt ttaccaatac
    12181 ccctcccgtc actgtcgcca gcggaatagc acttgtaccc ggcacgtttc atacacgggt
    12241 cactccttca ttactgttgc tgttactact ggcgatgcct ttgctgccag gcctttactg
    12301 gctgtggtgt cgttcgcgcc gcgcagcgtt tcgtcgcaca ggagatgcct gggcatgcgg
    12361 ctacggctgg gaaaacgcga tggctccgtc aggcaatggc gtgatgcagc cgctgcgtgt
    12421 ggtcttctct gcgttatttc gtctacgaca acagctcgac cctacgctga ggctaaataa
    12481 aggtcttgcg cacgtcaccg ccagggctca gagcacagaa cccttctggg atgagcgggt
    12541 gatccgcccc atcgtgagcg ccacccaacg gctggtcaaa gaaatacagc atctgcaaag
    12601 cggcgacttt cgtctctatt gcctgtatgt ggtcgccgca ctggttgtgc tgctaatcgc
    12661 tattgccgtc taaggaaatc accatgagac aaactctttg cgacggatat ctggtcattt
    12721 ttgcgttagc acaggccgtg attctgctga tgctaacccc actttttacg ggtatttccc
    12781 ggcagatacg cgcgcgtatg cactcccgcc gcgggccggg gatctggcag aattatcgcg
    12841 atatccacaa actgtttaaa cgccaggaag ttgcgccgac atcttcaggt ctgatgttcc
    12901 gcctgatgcc gtgggtatta atcagcagca tgctggtgct ggcgatggcc ttaccactat
    12961 ttattaccgt ttcccctttt gcgggcggcg gcgatctgat cacccttatc tatcttcttg
    13021 ccctgtttcg ttttttcttt gctctttccg ggctggatac cggaagtccg tttgcgggag
    13081 tcggtgccag tcgcgagttg acgctcggca ttctggtcga accaatgctt attctctcac
    13141 tgctggtatt ggcgctgata gcaggttcca cgcatatcga gatgatcagc aatacgctgg
    13201 ctatgggctg gaactcgccg ctaaccaccg tactggcgtt actggcctgt ggttttgcct
    13261 gcttcattga gatgggaaaa attccctttg atgttgctga agcagaacag gaattacagg
    13321 aaggcccgct gaccgaatat tccggtgccg ggctggcgct agcgaaatgg gggctggggc
    13381 tgaaacaggt cgtgatggca gcactgtttg tggccctgtt tctgcccttt gggcgcgcgc
    13441 aagaactttc tctcgcctgc ctgctgactt cacttgtcgt tacgctgctc aaggttttgc
    13501 tgatttttgt actggcctca atcgcagaaa acacgctggc acgcgggcgt tttttactca
    13561 ttcaccatgt gacctggctt ggcttcagcc ttgctgcgct tgcatgggtc ttttggttaa
    13621 ccggtctgta aggagcactg acggaatatg gaaaatcttg ctctgacgac gttaatgctg
    13681 ccttttatcg gcgcactggt cgtttcgttt tcgccacaac gtcgggccgc cgaatggggg
    13741 gttttgttcg ccgcgctgac cacgctgtgc atgttgtcgc tgatctccgc gttttatcag
    13801 gccgataaag ttgccgtcac gttgacgttg gtcaacgtgg gggatgtggc attatttggc
    13861 ctggtcattg atcgcgtgag tacgctgatt ctgtttgtgg tggtgtttct cggtttgctg
    13921 gtcacgatct actccacggg ttatctgacg gataaaaatc gcgaacaccc gcataacggc
    13981 acgaatcgtt attacgcatt tttgctggtg tttatcggcg cgatggcggg actggtactc
    14041 tcctcgacgc tgctcggtca gttgttgttt tttgaaatta cgggcggctg ctcctgggcg
    14101 ttgatcagtt attaccagag cgataaagcg cagcgttcag cactaaaagc gttgcttatc
    14161 acccatatcg gttcgttggg gttgtatctt gccgccgcca cgctgttttt gcagaccgga
    14221 acgtttgtgc ttagcgcgat gagcgagtta catggcgacg cacgttatct ggtttatggc
    14281 ggcatcctgt ttgccgcgtg ggggaaatcg gcccagctac cgatgcaagc gtggctaccg
    14341 gacgcaatgg aagcgccaac accgatcagc gcctatctcc acgccgcatc gatggtgaaa
    14401 gtgggcgttt acatttttgc ccgcgccatt atcgacggcg gcaatatccc gcatgtgatt
    14461 ggcggcgttg gcatggtcat gacactggtc accattcttt atggctttct gatgtatttg
    14521 ccacagcagg atatgaagcg gttgctggcc tggtcgacca tcactcaact tggctggatg
    14581 ttcttcggct tgtcgctctc catcttcggc tcgcggctgg cgctggaggg tagcatcgcc
    14641 tacatcgtca accacgcgtt cgctaaaagc ctgtttttcc ttgtagcagg tgcgctgagt
    14701 tacagctgcg gcacgcgctt gttgccgcgt ctgcgtggcg tattgcacac cctgccgttg
    14761 ccaggcgtgg atttctgcgt ggcagcgctg gcaattaccg gtgtgccgcc gttcaacggc
    14821 ttcttcagta aattcccgct gtttgccgcc ggttttgcgt tgtcagtgga gtactggatc
    14881 ctgctgcccg ccatgattct gctgatgatt gaatcggtcg ccagtttcgc ctggtttatt
    14941 cgctggtttg gtcgcgtcgt gccgggcaaa ccgagcgagg ccgtcgccga tgccgcaccg
    15001 ctgccagggt caatgcgcct ggtgttgatt gtactgattg tgatgtcgct gatttccagc
    15061 gtaatcgccg cgacctggtt gcagtaagga gatgatgaat gaccggttct atgatcgtaa
    15121 ataatctggc gggactgatg atgctgacat cgctgtttgt gattagcgtc aaaagctatc
    15181 gcctgtcatg cggattttac gcctgccagt cgctggtgct ggtgtctatt ttcgccactc
    15241 tctcgtgcct gttcgccgca gagcaactgc tgatctggtc cgccagcgcc tttatcacca
    15301 aagtgctgct ggtaccgtta atcatgactt acgctgcacg aaatattccc cagaacatcc
    15361 cggaaaaagc gttattcggt ccggcaatga tggcactgct cgcggcgtta attgtcctgc
    15421 tttgcgcatt tgtcgttcag cccgtgaagc taccgatggc taccgggctg aaaccggcgc
    15481 tggcggtagc gttaggtcat tttctgcttg gcctgctgtg cattgtcagc caacgcaata
    15541 tcctgcggca aatttttggt tactgcctga tggaaaacgg ctcccatctg gtgctggcgc
    15601 ttcttgcctg gcgagcaccg gaactggtgg aaataggtat cgctaccgac gccatcttcg
    15661 ccgtcattgt gatggtgtta ctggcaagaa aaatatggcg tacccacggc acgctggacg
    15721 tgaacaactt gaccgcgctg aagggataat gagatgagtt attctgtgat gttcgcttta
    15781 ctcctgctca cgccgctgct tttttcgctg ctctgttttg cctgccggaa acgggggctc
    15841 tctgcgactt gtacggtgac tgtattacat agcttaggga tcacactgct gctgattcta
    15901 gcactctggg tggtccaaac tgccgctgat gcaggagaaa tattcgctgc gggactgtgg
    15961 cttcatattg atggtctggg cggtttgttc ctcgccattc ttggtgtgat tggctttctc
    16021 accggtgttt actcgattgg ctacatgcgt catgaagtgg cacacggcga gctttcaccc
    16081 gttacactgt gcgattacta cggtttcttc catctgtttt tgttcaccat gctgctggtt
    16141 gttaccagca ataacctgat tgtgatgtgg gcggcgatcg aagccaccac cttaagctcg
    16201 gcgtttctgg taggcattta cggtcagcgt tcatcgctgg aatcagcatg gaagtacatc
    16261 attatttgta ctgttggtgt cgcttttggt ctgttcggta ccgtgctggt atacgccaac
    16321 gccgccagcg ttatgccgca ggcagaaatg gcgatattct ggagcgaggt tcttaagcaa
    16381 tcgtccttgc ttgacccaac attaatgctg ttggcctttg tgtttgtgct aattggcttt
    16441 ggtaccaaaa ccgggctatt tcccatgcac gcctggctgc cggatgctca cagtgaagcg
    16501 ccgagtccgg tcagcgccct gctctccgcc gtattgctga actgcgcgct gttggtgctg
    16561 attcgctatt acatcattat ttgccaggcc atcggcagcg atttccccaa ccggttgttg
    16621 ctcatcttcg gcatgttgtc ggttgccgtg gcggcatttt tcattctggt acagcgggac
    16681 attaagcgtc tgctggcgta ctccagcgtg gagaacatgg ggctggtcgc ggtggcgcta
    16741 ggcattggcg ggccgctggg aatttttgcc gcgctgctgc acaccttaaa ccacagtctg
    16801 gcaaaaacgc tgctgttctg cggttccggc aatgtactgc tcaagtacgg cacgcgcgat
    16861 ctcaacgtcg tctgtgggat gctcaaaatc atgccattta ccgccgtgct gtttggtggc
    16921 ggtgcgctgg cgctggcgct ggcagggatg ccgcccttca acatttttct tagcgaattt
    16981 atgaccgtta ccgcaggact ggcacgtaat cacctgctga ttatcgtcct gctgttattg
    17041 ctgttaacgc tggtgctggc gggcctggta cggatggctg cgcgggtgtt aatggcgaaa
    17101 ccgccgcagg ccgttaaccg gggtgatctc ggctggttga ccacctcgcc aatggtgatt
    17161 ctgctggtca tgatgctggc gatgggaacg catattccac aacctgtcat caggatcctg
    17221 gcgggcgctt ccactatagt cctctcaggg acgcacgacc tgcctgcaca acgtagcacc
    17281 tggcatgatt ttttgccttc aggcaccgca tctgtttcgg agaaacacag tgaacgttaa
    17341 ttcatcgtca aatcgtggcg aagcgattct cgccgccctg aaaacgcagt tccccggcgc
    17401 ggtgctggat gaagagcgac aaacgcctga acaggtcacc attacggtga aaatcaatct
    17461 gctgcctgac gttgtacatt atctttatta tcaacatgat ggctggcttc cagtcctgtt
    17521 tggcaacgac gagcggacac ttaacggtca ttacgcggtt tattatgccc tttcaatgga
    17581 aggggccgaa aaatgctgga tcgtggtgaa ggcactggtc gatgccgaca gtcgggagtt
    17641 tccgtcagtc acaccgcgcg tccctgccgc ggtctggggc gagcgagaaa ttcgcgatat
    17701 gtacgggctg attccggttg gcctgccgga tcagcgtcgc ctggtgttgc ccgatgactg
    17761 gccggaagat atgcatccgc tgcgcaaaga tgcgatggat tatcgactgc gccctgaacc
    17821 gacgacagat tccgaaacgt atgcgtttat caatgagggc aacagcgatg cgcgggtgat
    17881 ccctgtcggc ccgctgcata tcacctccta tgaaccgggt cacttccgct tgtttgtgga
    17941 tggcgagcaa attgtcgatg ctgattaccg cctgttttat gtccatcgcg gcatggagaa
    18001 actggcagaa acgcggatgg gctacaacga agtgaccttc ttatcggacc gcgtgtgtgg
    18061 gatttgcggt tttgcccaca gtgtggccta taccaactcg gttgaaaatg cactggggat
    18121 tgaggtgccg caacgagcac atactattcg ctcgattctg ctggaagtcg agcggctaca
    18181 cagtcatttg ctcaaccttg gcctctcctg ccattttgtt ggttttgata ccggctttat
    18241 gcaatttttc cgcgtgcggg aaaagtcgat gacgatggcg gaattgctga ccgggtcgcg
    18301 taaaacctac ggtctgaatc tgattggtgg tgttcgccgc gatattctca aagagcaacg
    18361 tctgcaaacg ctgaaactgg tgcgcgagat gcgcgccgac gtgtcggagc tggtagagat
    18421 gctgcttgct acgccgaata tggaacaacg cactcagggc attggcattc tcgaccgaca
    18481 aatcgcccgt gattatagcc ctgtagggcc gctgatccgc gcagtggttt tgcccgtgat
    18541 ttgcgctttg atcaccccta cgccgactac ggcaatattc caaaaacact gtttaccttt
    18601 accggcggcg atgttttctc ccgcgtgatg gtccgtgtca aagagacgtt tgattcgctg
    18661 gcaatgctgg aatttgccct cgacaacatg ccggataccc cactgctgac cgaaggcttt
    18721 agctataaac ctcacgcatt cgcgctgggc tttgttgaag cgccacgcgg tgaagacgtg
    18781 cactggagca tgctcggtga taaccaaaaa ttgttccgct ggcgctgtcg tgccgccacc
    18841 tacgccaact ggccggtgtt gcgttacatg ctgcgcggca ataccgtttc tgacgcaccg
    18901 ctgattatcg gtagccttga tccctgctac tcctgtaccg accgtgtgac gctggtagat
    18961 gtgcgcaagc gccagtcaaa aaccgtgctg tataaagaga tcgaacgcta cggcattgat
    19021 cgtaaccgtt cgccgctgaa gtaaggacag aagatgctga agttactgaa aactattatg
    19081 cgcgccggaa ccgcgacggt gaaatatccc ttcgcgccac tggaggtcag cccaggcttt
    19141 cgcgggaaac cggacctgat gcccagccaa tgtattgcct gcggtgcctg cgcctgtgct
    19201 tgtccggcaa atgcgctgac tatccagacc gacgaccagc aaaattcgcg cacctggcag
    19261 ctctatctgg ggcgttgtat ttactgcgga cgttgtgaag aagtgtgccc gaccagagcc
    19321 atccagctta ccaataactt tgaactgacc gtcacccata aagccgatct ctatacccac
    19381 gcgacgttcc atctacaacg ttgcagccgt tgcgaacgcc cgtttgcccc gcaaaaaacc
    19441 gtcgcactgg ctgctgaatt gttggcacag caacaaaatg ctccacaaaa ccgcgaaatg
    19501 ttatgggcgc aagcgagcgt ctgcccggag tgcaaacaac gcgcaacgct gatcaacgac
    19561 gatacagatg taccgctggt ggctaaggag cagctatgag tccagtgctt acgcaatatg
    19621 tcagccagcc catcacgctg gacgagcaaa cgcaaaagat gaagcggcat ttgctacagg
    19681 atatccgtcg ctcggcttac gtttatcgca tcgattgcgg cggctgcaac gcctgtgaaa
    19741 tcgaaatttt tgctgccatc acccccgtat tcgacgcaga acgttttggc attaaggttg
    19801 tttcatcacc gcgtcacgcc gatattttgt tatttactgg cgcagtcacc cgggcgatgc
    19861 gtatgcctgc acttcgggcg tatgagtctg cccccgatca taaaatctgt gtttcctacg
    19921 gcgcgtgcgg tgtcggcggc ggtattttcc acgatctcta cagcgtctgg ggcggcagcg
    19981 acaccattgt ccccattgat gtttggatcc ccggctgccc gccaacaccg gccgccacca
    20041 ttcacggttt cgccgtggcg ctcggtttgc tgcaacagaa gattcacgcg gtggattatc
    20101 gcgatcccac cggggtgact atgcaaccgt tgtggccgca gatcccgcca tcacagcgta
    20161 tcgccattga gcgagaagcg cggcggctgg cgggctatcg tcaggggcga gaaatttgcg
    20221 atcggctcct gcgccattta agcgacgatc ctacaggaaa tcgggttaac acctggttgc
    20281 gcgatgccga cgatccacgt ctcaatagta tcgttcagca actctttcgc gtactccggg
    20341 ggttacatga ctgaagagtg cggggaaatt gttttctgga cgctgcgaaa aaagtttgtc
    20401 gccagtagcg acgagatgcc ggaacacagc tctcaggtaa tgtattactc gctggctatc
    20461 ggtcatcacg ttggcgtgat tgattgtctg aatgtcgcct tccgctgccc actgacggaa
    20521 tacgaagatt ggcttgcact ggtcgaagag gagcaagccc gacgtaagat gctgggggtg
    20581 atgacttttg gtgagattgt tattgacgcc agccacaccg ccctgttgac ccgggcattc
    20641 gcgccactgg cagatgacgc gacgtctgtg tggcaagcgc gtagcattca attcattcat
    20701 ctgttggatg aaattgtgct ggaaccggct atctatctga tggccagaaa aattgcgtga
    20761 gaaggatttc tcattaataa ggactgttga tggctatgtc agacgaagcg atgtttgccc
    20821 cgccgcaagg aataacaatt gaagcggtaa acggaatgct cgcggagcgg ttagcgcaga
    20881 aacacggcaa ggcgtcttta ttacgcgcct tcatcccgct gccgccgccg ttcagcccgg
    20941 tacaacttat tgaactgcat gttctcaaaa gcaacttcta ttaccgctac catgatgatg
    21001 gcagcgatgt gacggcaaca acagagtatc agggcgagat ggtcgattat tcgcgttacg
    21061 ccgtccttct cggcagtagt ggaatggcgg agctacgctt tattcgtacc cacggcagcc
    21121 gttttactcc ccaggattgc acactgttta actggctggc acgcattatc accccggttc
    21181 tgcaatcatg gctcaatgat gaagcacagc aggtggcgct gcgtttgctg gagaaagatc
    21241 gcgatcatca tcgggtactg gtggatatca ctaatgcagt gctgtcacat cttgatctcg
    21301 acgatctgat cgctgacgtc gctcgtgaga tccatcattt tttcggtctg gcttcagtca
    21361 gtatggtact gggcgatcat cgaaagaacg agaagttcag cctgtggtgc agcgatcttt
    21421 ctgcctcaca ttgtgcgtgt ctgccacaca atatgcctgg cgacagtgta ttgctgacac
    21481 aaacgctaca aacccgacaa ccgaccttga cgcaccgtgc agatgatctg tttctctggc
    21541 aacgcgaccc gttattactc ttacttgcat ctaacggctg cgaatctgcg ctccttatac
    21601 cgcttacctt tggcaaccat acaccgggtg cattgttgct ggcgcatacc tcttccactc
    21661 tctttagtga ggaaaactgc cagctactac aacacatagc cgatcgcatc gctattgccg
    21721 ttggcaatgc cgatgcctgc cgtagaatga ccgatttgca ggaaagtttg cagcaagaaa
    21781 accaccagct tagcgagcag ctcctttcga atctgggcgt cggtgacatt atctatcaaa
    21841 gccaggcaat ggaagacctg ctccagcagg tagatattgt ggcgaagagc gacagtacgg
    21901 tgttgatttg cggtgaaacc ggaaccggca aagaggtgat cgccagagcg atccatcaac
    21961 ttagcccgcg acacgacaag ccgctggtca aaatcaactg cgctgccatc cccgccagtc
    22021 ttctggaaag tgagttattc ggtcatgaca aaggggcgtt tactggtgcg attaataccc
    22081 atcgtggtcg ttttgaaatt gccgatggcg gcacgttgtt tctcgatgaa attggcgatc
    22141 tgccgttaga acttcagcct aaactgctgc gcgtattgca ggaacgggag attgagcgtc
    22201 tcggcgggag tagaacgatc ccggtaaatg tcagagtcat tgccgccacc aaccgtgatt
    22261 tgtggcaaat ggttgaagat cgccagtttc gcagcgatct cttttatcgc ctgaatgtct
    22321 tcccactgga attgcctccg ctgcgcgacc gtccggaaga tatccctctt ttagcaaagc
    22381 atttcacgca aaaaatggcg cgccatatga atcgttcaat tgacgccatc ccgaccgagg
    22441 cgctacgcca gttgatgtcg tgggactggc cgggcaacgt gcgcgagctg gaaaacgtga
    22501 ttgagcgagc ggtactgctg actcgcggta acagtctgaa tttacatctt aatgtccgac
    22561 aaagccgttt actgccgacg ctaaatgaag attcagcgct tcgcagttca atggcgtagt
    22621 tgctgcaccc gacgacgcca gagaatgacg aagaagaacg tcagcgcatt gttcaggtat
    22681 tgcgagaaac caatggcatt gttgccgggc cccgtggcgc ggcgacacga ttagggatga
    22741 agcgcaccac gctgctgtca cgaatgcagc gtctgggaat ctcggttcgc gaggtgttgt
    22801 aatctgcttt tgcaggagta tgcatgagaa acaaactctc tttcgacttg cagttgagcg
    22861 ccagaaaagc ggcaatcgct gaacggattg ccgcccataa aattgcccgc agtaaagtgt
    22921 cggtcttttt aatggcgatg tccgctggcg tgtttatggc gatcggattt actttttacc
    22981 tttccgttat cgccgatgcc ccgtcttcat aggcattaac ccatctggtg ggcggccttt
    23041 gctttacact tggctttatt ttgctggcgg tttgcggcac cagcctgttc acctcgtcgg
    23101 taatgacggt aatggcaaaa agtcggggcg ttattagttg gcgaacttgg ctgattaacg
    23161 cacttctggt ggcctgcggt aatctggcag gtattgcctg tttcagtttg ttaatctggt
    23221 tttccgggct ggtgatgagt gaaaacgcga tgtggggagt cgcgatttta cactgcgccg
    23281 agggcaaaat gcatcataca tttactgaat ctgtcagcct cggcattatg tgcaatctga
    23341 tggtttgcct ggcgctgtgg atgagttatt gcggacgttc gttatgcgac aaaatcgtcg
    23401 ccatgatttt gtccatcacc ctgtttgtcg ccagtggctt tgagcactgt atcgccaatt
    23461 tgtttgtgat tccgttcgcc attgccattc gccatttcgc ccctacctcc ttctggcaac
    23521 tggcgcacag tagcgcagac aattttccgg cactgacggt cagccatttt attaccgcca
    23581 atctgctccc ggtgatgctg ggtaatatta tcggcggtgc agtgctggtg agtatttgtt
    23641 atcgggctat ttatttacgt caggaatcct gaaaaatcag cccggcgaaa cagttcgtcg
    23701 ggctgaagat tattcttgcg cgatttgccc atcgggccag gcgtggatca cggctttgat
    23761 cagcgtcgcc aatggaatgg cgaagaatac gccccagaat ccccacaaac caccgaagat
    23821 caccaccgat aaaataatca ccagcggatg caggttaacc gcttcggaga acaacaccgg
    23881 tactagcagg ttgccgtcca gcgcctgaat aatcagatac accgcgaagc agctccagaa
    23941 ttcagtacct gcgccaaact ggaatagcgc cacgccaacc accggaatgg tcaccacaaa
    24001 tgcgccaatg tacggaataa gaaccgagaa gccgaccagc actgccagca gcagcgaata
    24061 gttcagcccg aagagcaaga accccagcca ggtggcgatc cccaccacga tcatctccag
    24121 cactttgccg cggatatagt tggtgatttg ttgattcatc tccttccaca cctgtcctgc
    24181 cagcccacgg ttacgcggca gcacccggcg aacggcgttc agcatctgct ctttgtcttt
    24241 cagcaggaag aagaccatca atggcaccag caccagatag acggctatgg tcagcaaacc
    24301 gaccagcgag gcgagggaaa ttttcaccac cgaatcgccc atggtcagca tccgactgcg
    24361 catattttcg gccattgcat caataatgcc cgcgtccatt aacaccggat agcggcgcgg
    24421 caacgtggcg gcaaagtcag aaagcttatt gagcatcccc ggcatatcgc ggattaagta
    24481 gatgccctgt tgccaggcga taggcagtac cacgaacgcc atcagtagca atataccgac
    24541 aaaaaccacc aatacaatcg acgtcgccca gcggcgggag cagccaatag attgcaggcg
    24601 cacggttggc cattccagca aataagccag cacaatagcc accagcaacg gagcaagcag
    24661 gccactaaag aaaaagataa tgccaaatcc ggcaactaaa ataaccagca aggcaatcgc
    24721 ttccgggtcg ctaaaacggc ggcgatacca ttgcatcaac atttcgagca taaagacctt
    24781 ccctgaacct caagagcggg attgcgatcc gcaattgtat cgaaatgtca caaaaaagac
    24841 ttcgcttttt atgacggatt caggaaactg aaaagtcatt tgagtgggct aatcttcgcc
    24901 gttacactca aaggcggcga ggtgggaacg atatttcaca gtatcggtca aatgactatc
    24961 ttcagaaata caggatagag gttatgttca ggcagttgaa aaaaaacctg gttgcaaccc
    25021 tcattgctgc tatgaccatt ggtcaggtag ccccggcgtt tgccgacagc gcagacacct
    25081 tgccggatat gggaacctcc gcaggaagca cgctttccat tggtcaggaa atgcagatgg
    25141 gcgactatta tgtccgccag ctacgcggca gcgcgccgtt aattaatgac ccgctgttaa
    25201 cgcaatatat taattcgctg gggatgcgtc tggtttcgca tgccaattcg gttaagacac
    25261 cgtttcattt ttttctgatc aacaacgacg aaattaacgc ctttgctttc tttggcggca
    25321 acgtggtgct gcactctgcc ctgttccgtt attccgataa cgaaagtcaa ctggcttcag
    25381 ttatggcgca cgaaatctcc cacgtcaccc aacgtcacct ggcgcgagcg atggaagatc
    25441 aacaacgcag cgcgccgctg acctgggtcg gcgcgttagg ttctatttta ctggcgatgg
    25501 ccagtccgca ggcggggatg gcggcgctga ccggtacact ggcgggaacg cgccagggga
    25561 tgatcagttt cacccagcaa aatgaacagg aagcggaccg cattggtatt caggtgctgc
    25621 aacgctcggg attcgatcca caggcgatgc cgaccttcct ggaaaaattg ctcgatcagg
    25681 cgcgttactc ctcgcgcccg ccggaaattt tattgactca cccgttgccg gaaagtcgtc
    25741 tggcagatgc ccgcaaccgt gctaatcaga tgcgcccgat ggtggtgcag tcgtcggaag
    25801 atttctatct ggcgaaagcg cgcacactgg ggatgtataa ttccggacgt aaccagctca
    25861 ccagtgattt gctggatgaa tgggcgaaag gaaacgttcg tcagcaacga gcggcgcaat
    25921 atggtcgtgc tttacaggcg atggaagcca ataaatacga cgaggcgcgt aaaacgctgc
    25981 aaccgttact ggcggcagaa cctggcaacg catggtatct cgatctggct accgatatcg
    26041 atcttgggca aaacaaagcc aatgaggcaa tcaatcgcct gaaaaatgcc cgcgatttac
    26101 gcaccaatcc ggtgttgcag ctcaacctgg cgaacgctta tctgcaaggc ggtcaaccac
    26161 aagaagcggc caatattctg aatcgctaca cctttaataa taaagatgac agcaacggct
    26221 gggatttgct ggcacaggcg gaagccgcgc taaataaccg cgatcaggag ctggctgcgc
    26281 gagcagaagg ttatgcgctc gccggacgac tcgatcaggc catttcgctg ttgagtagcg
    26341 ccagttcgca ggtgaaatta ggcagcctgc aacaagcgcg ttacgatgcg cgcatcgacc
    26401 agttgcgcca gctgcagaaa cgctttaagc cttataccaa gatgtaattc gcattaaagg
    26461 aagaacaatg accaaacagg taaaaatcta ccataaccca cgctgttcaa agagccggga
    26521 aacgctgaac ctgctgaaag aaaacggcgt ggagccagaa gtggtgctct accttgagac
    26581 acccgccgat gcggcaacgc tgcgcgattt gctgaaaatg ctggggatga atagcgcccg
    26641 tgaattgatg cgccagaaag aggatcttta taaagaacta aacctggctg acagttcgct
    26701 tagcgaagag gcgctgattc aggcaatggt ggataatccg aagctgatgg agcgcccgat
    26761 tatggtcgcg aatggcaaag cgcggattgg tcgtccaccg gagcaggtac tggaaatcgt
    26821 gggttaagat gtgctccatc gctagttgaa gcacattgcc ggatgcgacg cttgccacgt
    26881 cttatccggc ctacgaatac tatggattaa cttcgcgtag gtcaggcgtt cgcgccgcat
    26941 ccgacaataa acaccttatc tacaacttca gaatttcttt cacaaacgga atggtcagct
    27001 tacgttgcgc ggtaatcgac gcacgatcca actgatccaa cgtcataaat agcgtgcgca
    27061 tttctctgtc gagccgcttc agcaagaaac gccccacatc ttccggcagt tcaaaaccac
    27121 gcaaacgcgc gcgtaactgt agcgcctgca acttatcttc atcagaaagt ggctgcaatt
    27181 tgtagatctg cccccagtcg agacgcgacg cgagatccgg taatcccaga ttcaactgcc
    27241 gcggtggacg atcgccggtg atcaacaacc gtgttttgcc cgattccaga attcgattgt
    27301 agagatcgaa aatcgccatc tcccacaact catcgcctgc aatacactca atgttgtcga
    27361 tacagaccag cgacaaatgc tccataccgt cgagcacttc cggaacaaac caggtgcgtt
    27421 tatccagcgg gacatagccc accgcatcgc cacgctgcga caattccgcg caagccgcgt
    27481 gcagcagatg gctgcgcccc gcgccttcgc gtgcccagag atagatgtaa ccgctatgtt
    27541 cctgacgcag cacgttttgc agcgcggcca gtaaagagga gttatccccc ggccagaaac
    27601 ttgcaaaggt ttcgtcgtca ggaagataaa gtggcaaaga gagctgtgcc ggtgtgttca
    27661 gagatacctc aaccaggatt tcacaaaatc gcgagaagtt taccacagaa tcccatgatg
    27721 tttgaaccgg gcagcaacac tgcccggtcg gtacattatt tgtctgttat atccgcgtct
    27781 tctgcgtcca gcaccacttc ttcagggcgc agcacactga tcagtttgaa aatcaaactt
    27841 aagccgatac cgacgatggt cgccagcgcc atacctttta actccgccgc gccgatgttt
    27901 accttcgcgc cactgacgcc gatgatcaaa atcacggaag tcaggatcag gttctgtgct
    27961 ttgttgtaat ccactttcga ttcgatcaaa acacgaatac cggaagcacc aatgacacca
    28021 taaagcagca gcgaaacgcc gcccatcacc ggcaatggga tcatctggat agcggcagcc
    28081 agtttaccga cgcaggaaag caggatagcg aaaatcgccg ccccgccgat aacccaggta
    28141 ctgtaaacac gggtgatcgc catcacgcca atgttttctc cgtaagtggt atttggcgta
    28201 gagccaaaga agccggagat cacggtcgac aggccgttag caaacatcga acggtgcaga
    28261 cctggatcgc gcagcagatc ttttttgacg atattagccg ttactaccag gtgccctacg
    28321 tgttcggcaa taaccactaa cgccgctggc agaatagtca gaatggcaaa ccactcgaag
    28381 cgcggcgtat agagggttgg cagtgcaaac cagtgagcat taataatcgg cgtggtatcg
    28441 acaattccca ttgcgaaaga gagcgcgtac cccaccagca cgccaattaa aatcgggata
    28501 attgccagga aaccacgaaa cagcacggaa cctaaaaccg tgaccgccag ggtggtaata
    28561 gagatgatga tggttttgga gtttggcgtt tgcccttcag ccgggagtaa acccgccata
    28621 ccggcagcta cgcccgccag ctccagaccg atgacggcaa cgattgcgcc cattgccgca
    28681 ggtggaaaca gcacgtccaa ccagccggtc cccgctttct tcacgataaa agaaaccagg
    28741 cagaacagca cgccgcacat aataaagccg cccagcgcga cttcataccc taacggcaac
    28801 agtaacaata ccggtgaaat aaaggcaaag ctggaaccaa gataagccgg aattttccct
    28861 ttacagatga agagatacag cagcgttcca ataccgttaa ataacagtac agtcgccggg
    28921 ttaatatgaa ataagacggg caccaggacg gttgcaccaa acatggcgaa caaatgttgc
    28981 aaactaagcg ggattgtctg taaaagtggc ggtctttcac tcaccccgat agcacggcgc
    29041 gtcatagtat tatcctctgt attatgtgtt ataggcgctt tactcaaaaa aaagccgact
    29101 cttaaagtcg gctttaatta tttttattct ttatttcgta ccaaagattt tgtcaccggc
    29161 atcgccgagg cccggaataa tgtatccgtg ctcgttcagt ccctggtcaa tcgatgcagt
    29221 atacagttcg acgtccgggt gcgctttttc cagcgcagcg ataccttctg gcgcagcgac
    29281 cagcaccaga actttgatgc tgctgcagcc cgcttttttc agcaggtcga tggtcgcgat
    29341 aacggaacca ccggttgcca gcattgggtc aacgatcagc gccatacgct catcgatgtt
    29401 agaaaccagt ttctggaagt acggtaccgg ctccagcgtt tcttcattac ggtacatacc
    29461 gacaacgctg atgcgcgcgc tcggaacgtt ttccagcaca ccgtccatca taccaagacc
    29521 cgcacgcaga attggcacaa cggtaatttt cttacctttg atctggtcga tttctaccgg
    29581 gccgttccag ccttcgatag ttactttttc cgtttcgagg tcggcggtcg cttcgtaagt
    29641 cagcaggcta cccacttcgg aagcgagttc gcgaaagcgc ttggtgctga tatcttgctc
    29701 acgcatcagt cccagcttgt gtttgacgag tgggtgtttg acttccacga tcttcatact
    29761 ctttctcctt tgaggggcag ccacaaaaaa atcgacggat tatacctcct ttcttcaggg
    29821 cggcaatatt attttcgttg actttagtca aaatgataac agtttgagat aaagttattt
    29881 tatattcaaa tggttatgaa agaagattat tccatccgaa aactaacctt tgccctggca
    29941 caagtcttct ttcgccgcgc gcccggggaa aagacgtgca aaaaggttgt gtaaaacagt
    30001 ctcgcaaacg tttgctttcc ctgttagaat tgcgccgaat tttatttttc taccgcaagt
    30061 aacgcgtggg gacccaagca gtgaccgata aaacctctct tagctacaaa gatgccggtg
    30121 ttgatattga cgcgggtaat gctctggttg gaagaatcaa aggcgtagtg aagaaaacgc
    30181 gtcgtccgga agtgatgggc ggtctgggcg gcttcggtgc gctgtgtgca ttgccgcaaa
    30241 aatatcgtga acccgtgctg gtttccggca ctgacggcgt aggtaccaag ctgcgtctgg
    30301 caatggactt aaaacgtcac gacaccattg gtattgatct ggtcgccatg tgcgttaatg
    30361 acctggtggt gcaaggtgcg gaaccgctgt ttttcctcga ctattacgca accggaaaac
    30421 tggatgttga taccgcttca gcggtgatca gcggcatcgc ggaaggttgt ctgcaatcgg
    30481 gctgttctct ggtgggtggc gaaacggcag aaatgccggg gatgtatcac ggtgaagatt
    30541 acgatgtcgc gggtttctgc gtgggcgtgg tagaaaaatc agaaatcatc gacggctcta
    30601 aagtcagcga cggcgatgtg ctgattgcac tcggttccag cggtccgcac tcgaacggct
    30661 attcgctggt gcgcaaaatt cttgaagtca gcggttgtga tccgcaaacc accgaacttg
    30721 atggtaagcc attagccgat catctgctgg caccgacccg catttacgtg aagtcagtgc
    30781 tggagttgat tgaaaaggtc gatgtgcatg ccattgcgca cctgaccggc ggcggcttct
    30841 gggaaaacat tccgcgcgta ttgccagata atacccaggc agtgattgat gaatcttcct
    30901 ggcagtggcc ggaagtgttc aactggctgc aaacggcagg taacgttgag cgccatgaaa
    30961 tgtatcgcac cttcaactgc ggcgtcggga tgattatcgc cctgcctgct ccggaagtgg
    31021 acaaagccct cgccctgcta aatgccaacg gtgaaaacgc gtggaaaatc ggtatcatca
    31081 aagcctctga ttccgaacaa cgcgtggtta tcgaataatg aatattgtgg tgcttatttc
    31141 cggcaacgga agtaatttac aggcaattat tgacgcctgt aaaaccaaca aaattaaagg
    31201 caccgtacgg gcagttttca gcaataaggc cgacgcgttc ggccttgaac gcgcccgcca
    31261 ggcgggtatt gcaacgcata cgctcatcgc cagcgcgttt gacagtcgtg aagcctatga
    31321 ccgggagttg attcatgaaa tcgacatgta cgcacccgat gtggtcgtgc tggctggttt
    31381 tatgcgcatt ctcagcccgg cgtttgtctc ccactatgcc gggcgtttgc tgaacattca
    31441 cccttctctg ctgccgaaat atcccggatt acacacccat cgtcaggcgc tggaaaatgg
    31501 cgatgaagag cacggtacat cggtgcattt cgtcaccgat gaactggacg gtggcccggt
    31561 tattttacag gcgaaagtcc cggtatttgc tggtgatacg gaagatgaca tcaccgcccg
    31621 cgtgcaaacc caggaacacg ctatttatcc actggtgatt agctggtttg ccgatggtcg
    31681 tctgaaaatg cacgaaaacg ccgcgtggct ggatggtcaa cgtctgccgc cgcagggcta
    31741 cgctgccgac gagtaatgcc cccgtagtta aagcgccagc tctgccgctg gcgtttttca
    31801 attcacctgt aaatcgcaag ctccagcagt ttttttcccc cttttctggc atagttggac
    31861 atctgccaat attgctcgcc ataatatcca ggcagtgtcc cgtgaataaa acggagtaaa
    31921 agtggtaatg ggtcaggaaa agctatacat cgaaaaagag ctcagttggt tatcgttcaa
    31981 tgaacgcgtg cttcaggaag cggcggacaa atctaacccg ctgattgaaa ggatgcgttt
    32041 cctggggatc tattccaata accttgatga gttctataaa gtccgcttcg ctgaactgaa
    32101 gcgacgcatc attattagcg aagaacaagg ctccaacgct cattcccgcc atttactggg
    32161 caaaattcag tcccgggtgc tgaaagccga tcaggaattc gacggcctct acaacgagct
    32221 attgctggag atggcgcgca accagatctt cctgattaat gaacgccagc tctccgtcaa
    32281 tcaacaaaac tggctgcgtc attattttaa gcagtatctg cgtcagcaca ttacgccgat
    32341 tttaatcaat cctgacactg acttagtgca gttcctgaaa gatgattaca cctatctggc
    32401 ggtggaaatt atccgtagcg atagcatccg ttacgcgctg ctggagatcc catcagataa
    32461 agtgccgcgc tttgtgaatt taccgccaga agcgccgcgt cgacgcaagc cgatgattct
    32521 tctggataac attctgcgtt actgccttga tgatattttc aaaggcttct ttgattatga
    32581 cgcgctgaat gcctattcaa tgaagatgac ccgcgatgcc gaatacgatt tagtgcatga
    32641 gatggaagcc agcctgatgg agttgatgtc ttccagtctc aagcagcgtt taactgctga
    32701 gccggtgcgt tttgtttatc agcgcgatat gcccaatgcg ctggttgaag tgttacgcga
    32761 aaaactgact atttcccgct acgactccat cgtccccggc ggtcgttatc ataattttaa
    32821 agactttatt aatttcccca atgtcggcaa agccaatctg gtgaacaaac cactgccgcg
    32881 tttacgccat atttggtttg ataaagccca gttccgcaat ggttttgatg ccattcgcga
    32941 acgcgatgtg ttgctctatt atccttatca cacctttgag catgtgctgg aactgctgcg
    33001 tcaggcttcg ttcgacccga gcgtactggc gattaaaatt aacatttacc gcgtggcgaa
    33061 agattcacgc atcatcgact cgatgatcca cgccgcacat aacggtaaga aagtcactgt
    33121 ggtggttgag ttacaggcgc gtttcgacga agaagccaac attcactggg cgaagcgcct
    33181 gaccgaagca ggcgtgcacg ttatcttctc tgcgccgggg ctgaaaattc acgccaaact
    33241 gttcctgatt tcacgtaaag aaaacggtga agtggtccgt tacgcacaca tcgggaccgg
    33301 gaactttaac gaaaaaaccg cgcgtcttta tactgactat tcgttgctga ccgcagatgc
    33361 gcgtatcacc aacgaagtac ggcgggtatt taactttatt gaaaacccat accgcccggt
    33421 gacatttgat tatttaatgg tgtcaccgca aaactctcgc cgtctgttat atgaaatggt
    33481 agaccgcgaa atcgccaacg cgcagcaagg gctgcccagt ggtatcaccc tgaagctaaa
    33541 taaccttgtc gataaaggcc tggttgatcg tctgtatgcg gcctccagct ccggcgtacc
    33601 ggttaatctg ctggttcgcg gaatgtgttc gctgatcccc aatctggaag gcattagcga
    33661 caacattcgt gccatcagta ttgttgaccg ttaccttgaa catgaccggg tttatatttt
    33721 tgaaaatggc ggcgataaaa aggtctacct ttcttccgcc gactggatga cgcgcaatat
    33781 tgattatcgt attgaagtgg cgacaccgct gctcgatccg cgcctgaagc agcgggtgct
    33841 ggacatcatc gacatattgt tcagcgatac ggtcaaagca cgttatatcg ataaagaact
    33901 cagtaatcgc tacgttcccc gcggcaatcg ccgcaaagta cgggcgcagt tggcgattta
    33961 cgactacatc aaatcactcg aacaacctga ataaccctat gccaatacac gataaatccc
    34021 ctcgtccgca ggagtttgct gcggtcgatc ttggttcaaa cagttttcac atggtcatag
    34081 cccgtgtggt agatggtgcc atgcagatta ttggccgcct gaaacagcgg gtgcatctgg
    34141 cggacggcct ggggccagat aatatgttga gtgaagaggc aatgacgcgc ggtttgaact
    34201 gtctgtcgct gtttgccgaa cggctacaag ggttttctcc tgccagcgtc tgtatagtcg
    34261 gcacccatac gctgcgtcag gcgctgaacg ccactgactt tctgaaacgc gcggaaaagg
    34321 tcattcccta cccgattgaa attatttccg gtaatgaaga agcccgtctg atttttatgg
    34381 gcgtggaaca tacccaaccg gaaaaaggtc gcaaactggt tattgatatt ggcggcggat
    34441 ctacggaact ggtgattggt gaaaatttcg aacctattct cgttgaaagc cgccggatgg
    34501 gttgtgtcag ctttgcccag ctttattttc ctggcggggt catcaataaa gagaattttc
    34561 agcgcgctcg catggcggca gcacaaaaac tggaaacttt aacctggcaa ttccgtattc
    34621 agggctggaa cgttgcaatg ggcgcttccg gtaccataaa agccgcccat gaagtgttaa
    34681 tggaaatggg cgagaaagac gggataatta ccccggaacg tctggaaaaa ctggtaaaag
    34741 aaattttacg tcaccgtaat ttcgcatcgc tgagtttacc gggtctttcc gaagagcgga
    34801 aaacagtctt cgttcctgga ctggcgattt tatgtggtgt gtttgatgct ttagccatcc
    34861 gtgaactgcg cctttctgac ggggcgttac gcgaaggcgt actgtatgaa atggaaggac
    34921 gtttccgtca tcaggatgtg cgtagtcgca ccgccagcag cctcgccaac cagtatcaca
    34981 tagacagcga acaggcccga cgagtgctgg ataccactat gcaaatgtac gaacagtggc
    35041 gggaacagca accgaagctg gcgcatccgc aactggaggc gctactgcga tgggccgcca
    35101 tgctgcatga ggtcgggttg aatatcaacc acagcggttt gcatcgccac tccgcttata
    35161 ttctgcaaaa cagtgacttg ccgggtttta atcaggaaca gcagctgatg atggcgacac
    35221 tggtgcgcta tcaccgtaaa gcgattaagc tcgacgatct gccgcgcttt accttgttta
    35281 agaagaaaca gttcctgcca ctgatacagc tattgcgcct tggcgtatta ctaaacaatc
    35341 aacgtcaggc aaccaccaca ccgccaacat tgacactgat tactgatgac agtcactgga
    35401 cactgcgttt cccgcatgac tggtttagtc agaatgcgct ggtactgctt gatctggaaa
    35461 aggagcaaga atactgggaa ggcgtggctg gctggcggtt gaaaattgaa gaagaaagta
    35521 ccccagaaat cgccgcttaa tgctttgcgg gccgacgaga aatgtcggcc cgcattattc
    35581 aggcactttc gcgaatgggt tcgatttcat tcagcgtatc aattaacggt tgcggcttac
    35641 caataagata accctgcata taatcgatcc ccaaagagag caccgcctcg cggatctctt
    35701 cgttttcaac gtactctgcc actacccgca ttttcttcat tcgcgccaga tggcaaatcg
    35761 acgccacaat ctgataatcc agactatttg acacaatatt gctgataaag ctgccgtcaa
    35821 ttttaagcag atcggcattc acatttttaa gccgtgcata gctggcgtag ccggtgccga
    35881 aatcatcaat cgcaatctgg cagcctaatt cctgaagatg ctgcaaggtg atttgcgcct
    35941 gcttaacatt ggtcagagca ttactttcgg tgacttcaaa aataagttgc cacgcttcaa
    36001 tctgatattt agccagcaac tgactgactt caacaggaaa acgagcctga cataccgagg
    36061 ttggagacag attaatagca aaacggtgag cgggcatctt cgctctgttt tcagccataa
    36121 attgcagcgt atgctcaatg acccacatgt cgatactcga cgataaacca aattcgtgcg
    36181 cgaccggcaa gaagctaccg gggccgatca gttcatcatt ctcacctttc atgcgcagaa
    36241 gaatttcatg gtaaacatca ccacgcatac cggtaatcgg ctgggccatc aggaaaaaat
    36301 ggttgtgttc cagcgcctgc tgtagtcgat tcatcatcgc gactttatct ttcaattcgc
    36361 gttgcaaata cattgccccg caacgctgca tattttccgg ggcgttggtc acgatggaaa
    36421 gttcggcgac cgtatttagc tctcccagca gcaggtagat atgattcact ggcgagcgca
    36481 catagcagta actgacgcca atctgcggtt gcatcggcat cccatcccaa aagaaacgaa
    36541 attgcttgag atgactatcc agtgcggtaa tgcgctcctg gtgcgattct gtattcagtc
    36601 gcagcgcgag atcgttaccc gaaagctgat aaacatcttc acccggttcc agcaagggtg
    36661 acagccagtg agaaagtttt tgctt
//
LOCUS       DAZSNO010000025        35312 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZSNO010000025 DAZSNO010000000
VERSION     DAZSNO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35312
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.025"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..971)
                     /locus_tag="ACHWN2_001207"
     CDS             complement(<1..971)
                     /locus_tag="ACHWN2_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8930825.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            1063..1149
                     /locus_tag="ACHWN2_001208"
                     /pseudo
     CDS             1063..1149
                     /locus_tag="ACHWN2_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1159..1239)
                     /locus_tag="ACHWN2_001209"
                     /pseudo
     CDS             complement(1159..1239)
                     /locus_tag="ACHWN2_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1554..2030)
                     /locus_tag="ACHWN2_001210"
     CDS             complement(1554..2030)
                     /locus_tag="ACHWN2_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR8930826.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(2089..3378)
                     /gene="bioA"
                     /locus_tag="ACHWN2_001211"
     CDS             complement(2089..3378)
                     /gene="bioA"
                     /locus_tag="ACHWN2_001211"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR8930827.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3465..4505
                     /gene="bioB"
                     /locus_tag="ACHWN2_001212"
     CDS             3465..4505
                     /gene="bioB"
                     /locus_tag="ACHWN2_001212"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR8930828.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4502..5656
                     /gene="bioF"
                     /locus_tag="ACHWN2_001213"
     CDS             4502..5656
                     /gene="bioF"
                     /locus_tag="ACHWN2_001213"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR8930829.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5643..6398
                     /gene="bioC"
                     /locus_tag="ACHWN2_001214"
     CDS             5643..6398
                     /gene="bioC"
                     /locus_tag="ACHWN2_001214"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR8930830.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6391..7068
                     /gene="bioD"
                     /locus_tag="ACHWN2_001215"
     CDS             6391..7068
                     /gene="bioD"
                     /locus_tag="ACHWN2_001215"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR8930831.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7647..9668
                     /gene="uvrB"
                     /locus_tag="ACHWN2_001216"
     CDS             7647..9668
                     /gene="uvrB"
                     /locus_tag="ACHWN2_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR8930832.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9860..10768)
                     /gene="yvcK"
                     /locus_tag="ACHWN2_001217"
     CDS             complement(9860..10768)
                     /gene="yvcK"
                     /locus_tag="ACHWN2_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR8930833.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      11033..11177
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11165..12154
                     /gene="moaA"
                     /locus_tag="ACHWN2_001218"
     CDS             11165..12154
                     /gene="moaA"
                     /locus_tag="ACHWN2_001218"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR8930834.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12176..12688
                     /gene="moaB"
                     /locus_tag="ACHWN2_001219"
     CDS             12176..12688
                     /gene="moaB"
                     /locus_tag="ACHWN2_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR8930835.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12691..13176
                     /gene="moaC"
                     /locus_tag="ACHWN2_001220"
     CDS             12691..13176
                     /gene="moaC"
                     /locus_tag="ACHWN2_001220"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR8930836.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13169..13414
                     /gene="moaD"
                     /locus_tag="ACHWN2_001221"
     CDS             13169..13414
                     /gene="moaD"
                     /locus_tag="ACHWN2_001221"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR8930837.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13416..13868
                     /gene="moaE"
                     /locus_tag="ACHWN2_001222"
     CDS             13416..13868
                     /gene="moaE"
                     /locus_tag="ACHWN2_001222"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR8930838.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            14005..14709
                     /locus_tag="ACHWN2_001223"
     CDS             14005..14709
                     /locus_tag="ACHWN2_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR8930839.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14914..15627
                     /locus_tag="ACHWN2_001224"
     CDS             14914..15627
                     /locus_tag="ACHWN2_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR8930840.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15663..16619)
                     /locus_tag="ACHWN2_001225"
     CDS             complement(15663..16619)
                     /locus_tag="ACHWN2_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFR8930841.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16619..17860)
                     /gene="clsB"
                     /locus_tag="ACHWN2_001226"
     CDS             complement(16619..17860)
                     /gene="clsB"
                     /locus_tag="ACHWN2_001226"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR8930842.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPRQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17857..18618)
                     /locus_tag="ACHWN2_001227"
     CDS             complement(17857..18618)
                     /locus_tag="ACHWN2_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR8930843.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18751..19161
                     /locus_tag="ACHWN2_001228"
     CDS             18751..19161
                     /locus_tag="ACHWN2_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR8930844.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(19123..20229)
                     /locus_tag="ACHWN2_001229"
     CDS             complement(19123..20229)
                     /locus_tag="ACHWN2_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8930845.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20240..21373)
                     /locus_tag="ACHWN2_001230"
     CDS             complement(20240..21373)
                     /locus_tag="ACHWN2_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8930846.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21366..23102)
                     /locus_tag="ACHWN2_001231"
     CDS             complement(21366..23102)
                     /locus_tag="ACHWN2_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8930847.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(23095..24090)
                     /gene="hlyD"
                     /locus_tag="ACHWN2_001232"
     CDS             complement(23095..24090)
                     /gene="hlyD"
                     /locus_tag="ACHWN2_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR8930848.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(24093..24764)
                     /gene="cecR"
                     /locus_tag="ACHWN2_001233"
     CDS             complement(24093..24764)
                     /gene="cecR"
                     /locus_tag="ACHWN2_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR8930849.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24993..26213
                     /gene="rhlE"
                     /locus_tag="ACHWN2_001234"
     CDS             24993..26213
                     /gene="rhlE"
                     /locus_tag="ACHWN2_001234"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR8930850.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(26207..26305)
                     /locus_tag="ACHWN2_001235"
                     /pseudo
     CDS             complement(26207..26305)
                     /locus_tag="ACHWN2_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26387..26869)
                     /gene="ybiA"
                     /locus_tag="ACHWN2_001236"
     CDS             complement(26387..26869)
                     /gene="ybiA"
                     /locus_tag="ACHWN2_001236"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR8930851.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26989..29139
                     /gene="dinG"
                     /locus_tag="ACHWN2_001237"
     CDS             26989..29139
                     /gene="dinG"
                     /locus_tag="ACHWN2_001237"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR8930852.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29167..30129
                     /gene="ybiB"
                     /locus_tag="ACHWN2_001238"
     CDS             29167..30129
                     /gene="ybiB"
                     /locus_tag="ACHWN2_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR8930853.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30270..31355
                     /gene="hcxB"
                     /locus_tag="ACHWN2_001239"
     CDS             30270..31355
                     /gene="hcxB"
                     /locus_tag="ACHWN2_001239"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR8930854.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            31422..31481
                     /gene="ybiE"
                     /locus_tag="ACHWN2_001240"
     CDS             31422..31481
                     /gene="ybiE"
                     /locus_tag="ACHWN2_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR8930855.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31584..31844)
                     /gene="ybiJ"
                     /locus_tag="ACHWN2_001241"
     CDS             complement(31584..31844)
                     /gene="ybiJ"
                     /locus_tag="ACHWN2_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR8930856.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(32109..32375)
                     /gene="ybiI"
                     /locus_tag="ACHWN2_001242"
     CDS             complement(32109..32375)
                     /gene="ybiI"
                     /locus_tag="ACHWN2_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR8930857.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPSVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32449..33126)
                     /gene="ybiX"
                     /locus_tag="ACHWN2_001243"
     CDS             complement(32449..33126)
                     /gene="ybiX"
                     /locus_tag="ACHWN2_001243"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR8930858.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33168..35177)
                     /locus_tag="ACHWN2_001244"
                     /pseudo
     CDS             complement(33168..35177)
                     /locus_tag="ACHWN2_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
     gene            35224..>35312
                     /locus_tag="ACHWN2_001245"
     CDS             35224..>35312
                     /locus_tag="ACHWN2_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930859.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLG"
BASE COUNT         8359 a         9042 c         9538 g         8373 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatccgtgg gccgtggtag tccggcgaag aggttaatct ttgcagggct
      181 tgcagagtgc gagtcgacaa tggattgtta taaatattaa catctgcata aatgtgcaga
      241 ttcatgatac tgtctggcaa tgtgttcagc tgattgttag ggatatatag tgattgcaga
      301 cgttgtggaa gttcgggcag tctgtgtagt tggttttccc caacgtttaa aagcctcaga
      361 ttttgaggaa ggggcggtag ctcttgtaat ctgttatcaa aagcacttag ttcctgtagt
      421 tcacatggta attctggtaa ggcagtaagc tggttgttgc caacagaaag tttttgtaga
      481 gccgggggaa gatcaggtag cgtctccagg ctattgagaa gggctgagag tgactgcaag
      541 gaagatggaa gagaaggcaa acatgttaaa actctgttaa gtgaaacatc aagagcgacc
      601 aggtgaggcg gaagagccgg gagcctgctc agtctgttat cgctggcttt aagtacagtt
      661 aaggacggag ggagttctgg cagagagcgt agctcattgc tagagatgtt aagttcttgt
      721 atgtgcgggg gcaggtatgg gagagagcgt aatctgagta aacttaaatt gagggctggc
      781 tcttgaaaag ccagacatat tttcagtaat cgaactgcct gtgttcggtc ttctgttgca
      841 gcaccttcct tggcccagtt atcccagatg cggtgataat ccgtgatatt ttgctcttct
      901 acggataaac gggatataca tcctaaacca caaaaggagc ggaaaaaaga aggattgtgg
      961 gcaggtttca tttattattg atcctcattg tttatatatt aatatattcg tgcaaatgga
     1021 gtgaggattc tttaaaaaaa cgcgcctgta aatagttaag aacaggaaga actgttgggg
     1081 atgtggctgg ccgaaaatga aggggtgaag ttctggctca atgtgctgac tgaactgaaa
     1141 aaacgcggtt ggtcgcccta ccttttcatc aagccagtag tctgcccacc acagcatcat
     1201 ttctctgcgc ttatcgagat actgagcatg gttgtaaatt cagctaaccc agtgcgttga
     1261 caaaaaatta gcgcaagagg acaaaaaatc accttgcgct aatgctctgt atcaggtcac
     1321 taataccatc taagtggttg attcatagtg actggatatg ttgtgttttg cagtattatg
     1381 cagtctattt tttaagctaa atgtattata atgcattgat attaatgatt tttactgttt
     1441 ctcggtcagc ttttttatac taacttgagc gaaacgggaa ggtaaaaaga caaaaagttg
     1501 tttttaatac ctttaagtga taccagatgg cattgcgcca tctggcagag tgattaacta
     1561 aacatcgcgg taatcgatgc gcttgccaga gagtggaaat gaacgttaaa cccgaccatc
     1621 gcgccgctgg ctccttcatc gacatcaata cgctctacat ccagcgcgtg aacggtaaaa
     1681 atgtagcgat gggtttcgcc tttcggcggc gctgcgccat cgtatccggt tttaccaaaa
     1741 tcggtacgcg tctgcaaaac gccgtctggc attgctacca gaccagagcc aaacccttgc
     1801 ggtaatacgc gggtatcagc gggtaaatta acaactaccc agtgccacca gccggagccg
     1861 gttggcgcat ccgggtcgta gcaggtgaca acaaaacttt tcgttcccgc aggaacatca
     1921 tcccacgcca gatgcggtga aatattatcg ccatcgtaac ccatgccgtt aaagacatga
     1981 cgatgcggca acttatcgcc atcgcgcaga tcgttactga tgagtttcat gaaccctcct
     2041 ttcttgtttg cagaaagtgt agccagaaac cctcacgctg acttcccgtt attggcaaaa
     2101 aaatgtttca tcctgtaccg cgcggttaac cgctgcggtc agacgctgca actgttgcgg
     2161 gagaataata tagggcggca tcaggtaaat cagtttgcca aaaggccgga tccatacccc
     2221 ctgttcgaca aagaattttt gcagcgccgc catattcacc ggacgagtgg tttcgaccac
     2281 accgattgcc cccagtacgc gcacatcggc aaccatttcg gcatcacggg ctggtgccag
     2341 ttgctcgcgc agctgcactt caatagccgc cacctgctgc tgccattcgc cggattcgat
     2401 aatcgctagg ctggcgtttg ctgccgcgca ggccagcgga ttgcccataa aagttggccc
     2461 atgcataaag cagccggctt cgccgttact gatggtttct gcaacctcgc gcgtggtaag
     2521 tgtggcggaa agggtcattg tgccgccggt taaggcttta ccgaggcaca aaatgtccgg
     2581 cgcgatttct gcatattcac aggcaaacag tttgccggta cgaccaaatc cggtggcgat
     2641 ctcgtcggca atcagcaaga taccttcgcg atcgcacatt ttgcggattc gttttaacca
     2701 ttccggatgg tacatgcgca tcccgcctgc gccctggaca atcggctcaa tgatcaccgc
     2761 tgcgatttca tgacgatgcg ccgccatcag gcgggcaaaa ccaaccatat cgcgttcatc
     2821 ccattcacca tccatgcggc tttgcggggc gggagcaaac aagttttctg gcagatagcc
     2881 tttccacagg ctgtgcattg agttgtccgg atcgcacacc gacatcgcgc caaaggtatc
     2941 gccatgataa ccattgcgga aggtcagaaa acgctggcgc gcttcgcctt tggcttgcca
     3001 gtactgcaac gccattttca tcgccacttc caccgctacg gaaccggagt ccgcgagaaa
     3061 aacgcactcc agcggttgcg gcgtcattgc caccagtttg cggcatagct cgatggcggg
     3121 cgcatgggtg ataccgccaa acatcacatg cgacatggca tcaatttgcg tcttcatcgc
     3181 cgcattaagc tgcgggtgat tgtagccgtg gatcgccgcc caccaggacg acataccgtc
     3241 aaccaggcgt ctgccgtcag acaaaattag ctcgcaacct tcggcgctcg ccaccggata
     3301 aaccggcaga ggggaggtca tggatgtgta tgggtgccag atatggcgtt ggtcaaaggc
     3361 aagatcgtcc gttgtcataa tcgacttgta aaccaaattg aaaagattta ggtttacaag
     3421 cctacaccga attaacaaca aaaaacacgt tttggagaag ccccatggct caccgcccac
     3481 gctggacatt gtcgcaagtc acagaattat ttgaaaaacc gttgctggat ctgctgtttg
     3541 aagcgcagca ggtgcatcgc cagcatttcg aacctcgtca ggtgcaggtc agcacgttgc
     3601 tgtcgattaa gaccggagct tgtccggaag attgcaaata ctgcccgcaa agctcgcgct
     3661 acaaaaccgg gctggaagcc gagcggttga tggaagttga acaggtgctg gagtcggcgc
     3721 gcaaagcgaa agcggcagga tcgacgcgct tctgtatggg cgcggcatgg aagaatcccc
     3781 acgaacgcga tatgccgtac ctggaacaaa tggtgcaggg ggtaaaagcg atggggctgg
     3841 aggcgtgtat gacgctgggc acgttgagtg aatctcaggc gcagcgcctc gcgaacgccg
     3901 ggctggatta ctacaaccac aacctggaca cctcgccgga gttttacggc aatatcatca
     3961 ccacacgcac ttatcaggaa cgcctcgata cgctggaaaa agtgcgcgat gccgggatca
     4021 aagtctgctc aggtggcatt gtgggcttag gcgaaacggt aaaagatcgc gccggattat
     4081 tgctgcaact ggcaaacctg ccgacgccgc cggaaagcgt accaatcaac atgctggtga
     4141 aggtgaaagg cacgccgctt gccgataacg atgacgttga tgcctttgat tttattcgca
     4201 ccattgccgt agcgcggatc atgatgccga cctcttacgt gcgcctttct gccggacgcg
     4261 agcagatgaa cgaacagact caggcgatgt gctttatggc aggcgcaaac tcgattttct
     4321 acggttgcaa actgctgacc acgccgaatc cggaagaaga taaagacctg caactgttcc
     4381 gcaaactggg gctaaatccg cagcaaactg ccgtgctggc aggggataac gaacaacagc
     4441 aacgtcttgg acaggcgctg atgaccccgg acaccgacga atattacaac gcggcagcat
     4501 tatgagctgg caggagaaaa tcaacgcggc gctcgatgcg cggcgtgctg ccgatgccct
     4561 gcgtcgccgt tatccggtgg cgcaaggagc cggacgctgg ctggtggcgg atgatcgcca
     4621 gtatctgaac ttttccagta acgattattt aggtttaagc catcatccgc aaattatccg
     4681 tgcctggaag cagggggcgg agcaatttgg catcggtagc ggcggctccg gtcacgtcag
     4741 cggttatagc gtggtgcatc aggcgctgga agaagaactg gccgagtggc ttggctattc
     4801 gcgggcgctg ctgtttatct ctggttttgc cgcgaatcag gcagtcattg ccgcgatgat
     4861 ggcgaaagag gaccgtattg ttgccgaccg gcttagccat gcctcattgc tggaggctgc
     4921 cagtttaagc ccgtcgcagc ttcgccgttt tactcataac gatgtcgctc atctggcgcg
     4981 actgcttgct tccccctgtc cggggcagca actggtggtg acagaaggcg tgttcagcat
     5041 ggacggcgat agcgcgccac tggcggaaat ccagcaggta acgcaacagc acaatggctg
     5101 gttgatggtc gatgatgccc acggcacggg cgttatcggg gagcaggggc gcggcagctg
     5161 ctggctgcaa aaggtaaaac cagaattgct ggtagtgact tttggcaaag gatttggcgt
     5221 cagtggggct gcggtgcttt gctcaagtac ggtggcggat tatctgctgc aattcgcccg
     5281 ccaccttatc tacagcacca gtatgccgcc cgctcaggcg caggcattac gtgcgtcgct
     5341 ggcggtcatt cgccgtgatg agggggatgc acggcgcgaa aaactggtgt cactcattgc
     5401 gcgttttcgt gccggagtac aggatttgcc gtttacgctg gctgattcat gcagcgccat
     5461 ccagccattg atcgtcggtg ataacagccg tgcgttacaa ctggcagaaa aactgcgcca
     5521 gcaaggctgc tgggtcacgg cgattcgccc gccaaccgta cccgctggta ctgcgcgact
     5581 gcgcttaacg ctaaccgctg cgcatgaaat gcaggatatc gaccgtctgc tggaggtgct
     5641 gcatggcaac ggttaataaa caagccattg cagcggcatt tggtcgggca gccgcacact
     5701 atgagcaaca tgcagatcta cagcgccaga gtgctgacgc cttactggca atgcttccac
     5761 agcgtaaata cacccacgta ctggacgcgg gttgtggacc tggctggatg agccgccact
     5821 ggcgggaacg tcacgcgcag gtgacggcgt tagatctctc gccgccaatg cttgcgcaag
     5881 cccgccataa agatgccgca gatcattatc tggcaggaga tatcgaatcc ctgccgttag
     5941 cgactgcgac gttcgatctt gcatggagca atctcgcggt gcagtggtgc ggtaatttat
     6001 ccacggcact ccgcgagctg tatcgggtgg tgcgccccgg aggcgtggtc gcgtttacca
     6061 cgctggtgca gggatcgtta cccgaactgc atcaggcgcg gcaggcggtg gacgagcgtc
     6121 cgcatgctaa tcgcttttta ccgccagatg aaatcgaaga gtcgctgaac ggcgtgcgtt
     6181 atcaacatca tattcagctc atcacactat ggtttgatga tgcgctcagt gccatgcgtt
     6241 cgctgaaagg catcggtgct acgcatcttc atgaagggcg cgacccgcga atattaacgc
     6301 gttcgcagtt gcagcgattg caactggcct ggccgcaaca acaggggcga tatcctctga
     6361 cgtatcatct ttttttggga gtgattgctc gtgagtaaac gttattttgt caccggaacg
     6421 gataccgaag tggggaaaac cgtcgccagt tgtgcacttt tacaagccgc gaaggcagta
     6481 ggttatcgga cggcaggtta taaaccggtc gcctctggca gcgaaaagac cccggaaggc
     6541 ttacgtaata gtgacgcgct ggcgttacag cacaacagca gcctgcagct ggattacgca
     6601 acggtaaatc cttacacctt cgcagaatcc acctcgccgc acatcatcag cgcccaagag
     6661 ggcaggccga tagaatcatt ggtaatgagc gccggattac gcgcgcttga acaacaggct
     6721 gactgggtgt tagtggaagg tgctggcggc tggtttacgc cgctttctga cactttcact
     6781 tttgcagatt gggtaacaca ggaacaactg ccggtgatac tggtagttgg tgtgaaactc
     6841 ggctgtatta atcacgcgat gttgactgcg caggcaatac aacacgccgg actgactctg
     6901 gcgggttggg tggcgaacga tgttacgcct ccgggaaaac gtcacgctga atatatgacc
     6961 acgctcaccc gcatgattcc cgcgccgctg ctgggagaga tcccctggct tgcagaaaat
     7021 ccagaaaatg cggcaaccgg aaagtacata aaccttgcct tgttgtagcc attctgtatt
     7081 tggttaaatt gcgagcgaga tcgcgtcttc gattgactgc aatttaacca attaaattct
     7141 aaaataatca cgaaaaaaat tttacttccg cctcatgcgg cgaatgtggg aattgcccag
     7201 gcggcggggg ataggggctg gagacagtta tccactattc ctgtggataa ccatgtgtat
     7261 tagagttaga aaacacgagg caagcgagag aatacgcggc ttgcacgcga attggcgtta
     7321 aagacggctc aaagaaatat cttttatttt ttaactggtt agataaatgc aatggcagtc
     7381 actgaacagg catctcttgc cataaaactg tcatcactca tcttgacaaa tgttaaaaaa
     7441 gccgttgctt tggggataac ccggtaaggc tggagtttta tctcgccaca gagtaaattt
     7501 tgctcatgat tgacagcgga gtttacgctg tatcagaaat attatggtga tgaactgttt
     7561 ttttatccag tataatttgt tggcataatt aagtacgacg agtaaaatta catacctgcc
     7621 cgcccaactc cttcaggtag cgactcatga gtaaaccgtt caaactgaat tccgctttta
     7681 aaccttctgg cgatcagcca gaggcgattc gacgtctcga agaggggctg gaagatggcc
     7741 tggcgcacca gacgttactt ggcgtgactg gctccgggaa aaccttcacc attgccaatg
     7801 tcattgctga ccttcagcgc ccaaccatgg tacttgcgcc caacaaaacg ctggcggccc
     7861 agctgtatgg cgaaatgaaa gagttcttcc cggaaaacgc ggtggagtat ttcgtctcct
     7921 actacgacta ctatcagccg gaagcctatg taccgagttc cgacaccttc attgagaaag
     7981 atgcctcggt taacgaacat attgagcaga tgcgtttgtc cgccaccaaa gcgatgctgg
     8041 agcggcgtga tgtgattgta gtggcgtctg tttccgcgat ttatggtctg ggcgatcctg
     8101 atttatatct caagatgatg ctccatctca cggtcggtat gattatcgat cagcgcgcga
     8161 ttctgcgccg actggcggag ctgcaatacg ctcgtaatga tcaagcattc cagcgtggta
     8221 ctttccgcgt tcgtggcgag gtgatagata tcttcccggc agaatcggat gacattgcac
     8281 ttcgcgtgga actgtttgac gaggaagtgg aacgattgtc gttatttgac ccgctgaccg
     8341 ggcagattgt ttccactatt ccacgtttta ccatctaccc gaaaacgcac tacgtcacac
     8401 cgcgcgagcg catcgtacag gcgatggagg agatcaaaga agaactggcc gccagacgca
     8461 aagtgctgtt ggaaaacaac aaactgctgg aagagcagcg gctgacccag cgtacccagt
     8521 ttgatctgga gatgatggac gagctgggct actgttcggg gattgaaaac tactcgcgct
     8581 tcctctccgg tcgtggaccg ggtgagccac cgccgacgct gtttgattac ctgcctgccg
     8641 atgggctgct ggtcgtcgat gaatctcacg tcaccattcc acaaattggc ggcatgtatc
     8701 gcggtgaccg ggcgcgtaaa gagacactgg tggagtacgg cttccgcctg ccatcagcgc
     8761 tggataaccg tccgcttaag tttgaagagt tcgaagcatt agcgccgcaa accatctatg
     8821 tttcggcgac gccgggtaat tacgagctgg aaaaatccgg cggcgatgtg gtggatcagg
     8881 tggtgcgtcc aaccggattg cttgacccga ttatcgaagt gcggccggtg gcgacacagg
     8941 ttgatgatct tctttcggag attcgtcagc gagcggcaat taacgaacgc gtgctggtca
     9001 ccacactgac caagcgaatg gcggaagatc ttaccgaata tctcgaagaa cacggtgagc
     9061 gcgtgcgtta tcttcactca gatatcgaca ccgtcgagcg tatggagatt atccgcgact
     9121 tgcgtctggg tgagttcgac gtattggtag ggatcaactt actgcgtgaa ggtctggata
     9181 tgccggaagt gtcgctggtg gcgatcctcg acgctgacaa agaaggcttc ctgcgttccg
     9241 aacgttcgtt gatccagacc attggtcgtg cggcacgtaa cgttaacggt aaagcgattc
     9301 tctacggcga taggagcact ccatcaatgg cgaaagcgat tggcgaaacc gaacgtcgcc
     9361 gtgagaaaca gcagaagtac aacgaggaac acggcattac gccgcaaggc ttgaacaaga
     9421 aagtggtcga tatcctggcg ctggggcaga acattgccaa aaccaaagcg aagggcagag
     9481 gaaaatcgcg cccgattgtt gagccggata atgtgccgat ggatatgttg ccgaaagcgt
     9541 tgcagcagaa aatccatgaa ctggaagggc tgatgatgca acacgcgcag aatctggagt
     9601 tcgaagaagc ggcacaaatt cgtgaccagt tgcatcagtt gcgtgagttg tttattgccg
     9661 catcgtaaca ggatagcgaa gaagactgat gacaaacgga aaacagtctg atgcgctacg
     9721 cttatcaggc ctacattttc tccgcaatat attgaatttg cgcggtttgt aggccggata
     9781 aggcgttcac gccgcatccg gcataaacaa cgcgcacttt gtcttcaata ggaagccgga
     9841 attttccttc ggatttccgt taacctaaag cctgtaacgc cttttccagc gcgttatgta
     9901 acaactggcg gtcatgacga tacggaatat cgctggcctc cagtacctcc tggatcacaa
     9961 tccgctcttt caccgccgag acatccactt ttggcccgac gatgaccgca tcaatgacct
    10021 ttttaccaac atactgctcc ataattgcca gcttgctttc cagcttcaaa ttagccgcag
    10081 gtaaactcaa ctcacgcccc agattgccga tataaaccat cggcgctggc gtgcggcgta
    10141 atgcctgggc gatttccttc agcagcagaa ttggcatcag gctggtataa aaactgccag
    10201 gcccaataat gatgagatcc gcttcattga tagcgtgaac cgcctcacgc gttgcgggta
    10261 cattaggcgt taacaataac tcttgaatcg gcgtagttaa ctggtcgata ttgacctcgc
    10321 cgtaaacttc atgcccctga tcgtcaatcg ccatcagatc aacaggatgc tctgacattg
    10381 gaatcaaatg cgtatccact ttcagcagat tacgaattaa attgatggct tccagaggcc
    10441 gcacgctaag gtgatccagc gcctttaaca tcaagtttcc gagattatga ccggaaagtt
    10501 cgccattgcc gccaaaacgg tattcaaaca tcgcggaggc gacgttcggt tccgttatca
    10561 gctggttgag gcagttgcgc atatcgcccc aagcaatgcc gccttctgaa cggcgaatac
    10621 gccccgtcga gccaccatta tccgtggtgg tgacgatacc cgttaaacga gaacccaaag
    10681 acgaaagtga tgagagaacg cgtcccagtc catgccctcc gccgagagca acgacacgat
    10741 caagatcagc cagcgtacga ttgcgcatat agtttcctgg agtcagatta tccgcgctac
    10801 agtagcgcaa attgcgggga aacagcaatt aacctgccaa tattgaggat acaaactatt
    10861 ttcttctatc tctaaaggac gatgcacgct atgcctccct gatgatgtat atcaaagtaa
    10921 aaccgccatt ttcccttatt ctgtagcgaa atagcacgat catgacgcta tatatatgat
    10981 tacatagcga aagtgtggat ggtaaaaatc tcatttacac gctagtatcg gcttaaccac
    11041 taaacactct agcctctgca cctgggtcaa ctgatacggt gctttggccg tgacaatgct
    11101 cgtaaagatt gccaccaggg cgaaggaaga aatgacttcg cctcccgtat ctggaaaggt
    11161 gtacatggct tcacaactga ctgatgtatt tgcgcgtaag ttttactact tgcgcctgtc
    11221 gattaccgat gtgtgtaact ttcgttgcac ctactgcctg ccggatggct acaaaccgag
    11281 cggcgtcacc aataaaggct ttcttaccgt cgatgaaatt cgccgggtta cgcgcgcctt
    11341 cgccagtctg ggcaccgaaa aagtgcgcct gacaggagga gagccgtctt tacgccgcga
    11401 ctttaccgat atcatcgccg ctgtgcggga aaacgacgct atccgccaga ttgcggtcac
    11461 caccaatggt taccgtctgg aacgcgatgt ggcgaactgg cgcgatgcgg gacttactgg
    11521 cattaacgtc agtgtcgaca gtttggacgc ccgccagttt cacgctatta ccgggcagga
    11581 taaattcaac caggtcatgg cagggattga tgctgcattt gaggccggtt ttgagaaggt
    11641 caaagtcaat accgtgctga tgcgtgatgt taatcatcac cagctcgaca cctttctgaa
    11701 ctggatccag catcgcccta tccagctgcg tttcatcgaa ctgatggaaa cgggcgaggg
    11761 cagcgagctc ttccgtaagc atcacatctc tggtcaggtt ctgcgtgacg agctactgcg
    11821 tcgcggctgg atccaccaat tacgtcaacg cagcgacggt cccgcgcaag tcttttgcca
    11881 tccagattac gccggagaga ttggccttat catgccgtat gaaaaagact tctgcgccac
    11941 ttgcaaccgc ctgcgcgttt cctccattgg taaactccat ctctgcctgt ttggtgaagg
    12001 cggcgttaac ctgcgcgatc tgctggaagg cgatacccag caacaggcgc tggaagcgcg
    12061 tatttcagcg gcgctgcggg agaagaaaca gacccatttc ctgcatcaaa acaacaccgg
    12121 tattacgcaa aacttatcgt acattggcgg ctaaaacgtc aaaaggagag atcagatgag
    12181 tcaggtaagc actgaattta tcccgacccg tattgctatt cttacggttt ctaatcgtcg
    12241 cggtgaagaa gacgatacct ccggtcacta tctgcgcgat tcggcgcaag aagcgggcca
    12301 tcacgttgtc gatgaagcca ttgtgaaaga aaaccgctac gctattcgcg ctcaggtatc
    12361 tgcgtggatc gccagcgacg atgtacaagt ggtattgatt acgggtggta ctggcctgac
    12421 ggaaggtgat caggctcccg aagcgctgct gccgttgttc gaccgtgagg ttgaaggttt
    12481 tggtgaagtg ttccgtatgt tgtcgtttga agagattggc acttccacgt tgcaatctcg
    12541 tgcggtagcg ggcgtcgcta acaaaacgct gattttcgcc atgccgggtt cgaccaaagc
    12601 gtgccgtacc gcatgggaaa atatcatcgc gccgcagctg gatgcccgta cgcgtccgtg
    12661 taatttccat ccacatttga agaaataagt atgtcgcaac tgacccatat caacgccgct
    12721 ggcgaagcgc acatggtgga tgtctccgcc aaagcggaaa ccgtgcgtga agcgcgggcg
    12781 gaagcctttg tcaccatgcg cagcgagacg ctggcgatga ttattgatgg tcgccaccac
    12841 aaaggcgacg tatttgccac tgcgcgtatt gccggtattc aggcggcaaa acgcacttgg
    12901 gatctgatcc cgctgtgtca cccgctgatg ctcagcaaag ttgaagtcaa tttacaggcc
    12961 gagccggagc acaatcgggt gcgtatagaa accttatgcc gcctgaccgg gaaaaccggt
    13021 gtcgaaatgg aagcgttaac cgcggcctcc gtggcggcgc tgaccattta tgacatgtgc
    13081 aaagcggtgc aaaaagatat ggtgattggt ccggtacgtt tgctggcgaa gagcggcggc
    13141 aagtcgggtg actttaaggt ggaagcggat gattaaagtt ctttttttcg cccaggtgcg
    13201 cgagttggtg ggaacagatg caaccgaagt ggctgcggat tacccaaccg ttgaagcgtt
    13261 acgccagcac ctggctgcgc agagcgatcg ctgggcgctg gcgctggaag atggcaaatt
    13321 gctggctgcc gtcaaccaga cgctggtgag ttttgaccat ccgctgactg acggcgacga
    13381 agtagctttc ttcccgccgg taaccggagg ttaagatggc agaaaccaaa attgttgtcg
    13441 gcccgcagcc gttcagcgta ggagaagagt acccgtggct ggcggagcgt gacgaagacg
    13501 gtgcggtagt caccttcacc ggaaaggtgc gcaaccataa cctgggcgac agcgtcaaag
    13561 cattaaccct cgaacactat ccggggatga ctgaaaaagc actggcagaa attgttgatg
    13621 aagcgcgtaa ccgctggccg ctggggcgcg tcacggtgat tcaccgcatc ggggaattat
    13681 ggccgggcga tgaaatcgtt tttgtcggtg tcaccagtgc gcatcgcagc agtgcgtttg
    13741 aagccgggca gtttattatg gattatctca aaacccgcgc accgttctgg aagcgcgaag
    13801 ccacgccgga aggcgaccgc tgggttgaag cccgggagag cgatctgcag gcggcaaaac
    13861 gctggtagtt ttttgttagc cggataaggc accgctgcgt ccggcaattt caccgctcag
    13921 tacatgcgtt tacgctgcgt cgatgcacag cctcatcatt ttgcagtatc cttaagatat
    13981 tccttatatc ttcaggagat cgtcatggac agattcccac gttctgattc aatcgtacaa
    14041 ccctgggctg gcttgcaaac ttatatggcg caagtctatg gctggatgac cgttggcttg
    14101 ttgctgaccg catttgttgc ctggtatgcg gctaattccg cggccgtgat ggagctgttg
    14161 ttcactaacc gtgtcttttt aatcggtctg atcatcgcgc aattagcatt ggttattgtg
    14221 ttatcagcga tgattcaaaa gctgagcgca ggtgtaacga cgatgctctt tatgctttat
    14281 tcggcgctga cgggtcttac gctttccagt atattcattg tctataccgc tgcttctatc
    14341 gccagtactt tcgtcgttac tgccgggatg ttcggcgcaa tgagcctgta cggttacacc
    14401 acgaagcgcg atttaagtgg ctttggcaat atgctgttta tggcgttaat cggcattgtg
    14461 ctggcatcgc tggtcaactt ctggctgaaa agcgaagcat tgatgtgggc agttacctac
    14521 atcggcgtga ttgtctttgt cggattgacg gcgtatgaca ctcagaaatt gaaaaatatg
    14581 ggtgagcaga ttgatacccg cgacacgtcg aacctgcgca aatattccat tcttggtgcg
    14641 ttaaccttgt atctggactt catcaacctg ttcctgatgt tgttacggat cttcggcaac
    14701 cgccgttaat tctttttcac catcactatc ccggccatgt gtcgggattt ttttcttact
    14761 aaaaacgaca cttaacaagt accaggtata aaatatgcct tatgagcgag cggaatcgtt
    14821 ttccgtatcc ataaatacaa aagtattttg tatgcgtggt tttaaattta ttgatggtga
    14881 atattaatgt tttttctaag gacaactaaa agaatggaat catactcgca aaacagcaat
    14941 aaattagatt ttcagcacga ggccaggata ttaaacggta tatggctcat taccgcttta
    15001 ggtttggtgg caaccgcagg actagcctgg ggagctaagt atctcgaaat tacggcaacc
    15061 aaatatgatt caccaccaat gtatgtcgcc atagggttat tattactttg tatgtatggc
    15121 ttaagtaagg atatcaacaa gataaatgcc gccatcgcgg gcgtaatata tctgttttta
    15181 ctctctttgg tggcgattgt cgttgcaagt ttagttcctg tatatgccat tatcatcgtg
    15241 ttcagcactg cgggcgcgat gtttttaatc agtatgctgg ccggtttatt atttaatgtt
    15301 gatcctggtt ctcaccgttt tatcattatg atgacgttga cagggttggc cctggtaatc
    15361 atcgtgaatg cggcattaat gagtgaacgg cccatttggg taataagttg cttaatgatt
    15421 gtgttatggc caggcattat ctcgcatgga cgaaataagc tccttgaact ggcaggaaaa
    15481 tgccatagtg aagagttgtg gagtccggtt cgttgcgctt ttacaggtgc attaacactc
    15541 tattactatt ttatcggctt ctttgggata cttgccgcga tagctataac gcttgtctgg
    15601 caaaggcata cgcgtttttt tcattagtcg taaatgcaga tactaaagaa cttaactaaa
    15661 cttcacatcg ccgcttcatt tttcgcccgt agcttcttcg cttggctttc cagtagcagg
    15721 taacagatca gcgccagcag cagcgggata aagtaataca ggacgcgata agcgagcagg
    15781 gcggcgataa tgctcccttt cgaggtatgc tccccagcca gtagtgcgat aaacaccgct
    15841 tccagcacac cgatccccgc cggaatatgc acgatgacgc cagcaatact actaaccagt
    15901 aacacgccca gtacaaagaa atagttcacg ctttgaccaa gtaacagcca gataatcgcc
    15961 cccattacca tccagttaac gctggaaatc agcatctgag cgagggcgaa tttccatgaa
    16021 ggcagcacca gcttttgccc tttgatggtc atatggcggt gcttcgcgaa agcgcaaaac
    16081 cacaaataaa ccgcgataat catcagtaag ccaatgccga gaatacgcag cgtagtttga
    16141 tcgacatacc agtaatccgg caactccacc acgcctgcgg taaagataat ccctgccagt
    16201 aaaatatagc ccagccagtt ggtggtaata ctgagcgagg aaatccgcgt aatagtgctg
    16261 cccggtaacc ctagtcggga gtacaaacga tagcgcatac caatgccgcc gacccaggta
    16321 ctgagcgtca ggttgaaggc gtagcagata aacgacacca gcatcacctg gcgcttcgcc
    16381 agtttgtgac cgcagtaaaa gcgggcgagc aggtcatagc agccgtaaat cagatagctg
    16441 acgaccacca gcccgaccgc actaagcagc gcaacgcgat tgtagtcgcg gatgaccttc
    16501 cagacctctt cccagtccac ttttttggcg tagaccacca gcaacacaat caccgcgata
    16561 aaaaacagcc aggtgaggat cttctttgct aagcgccagc gcgggtgtga tttactcatc
    16621 aggggtttac ccccgtgttt tcagtttcta cccgatcctg cgtttccatt gtcggttgtg
    16681 cggggggatc aacctgcgtc agacgtggtg tgtgtgccgg aagccagcca accagcgccg
    16741 ggaagtggcg taaaaagtgg aacgccagca cgcttttggt caggttccac caggtgcgtt
    16801 tgggcagcat ggtttcatcc acctgctgac aatctgcggc aataatgccg ttcagattat
    16861 cgcgcagcgt ctggttaaaa tgacgatcgt ggatgatgac atttgcttcg agattcagtg
    16921 acaaactgag cggatcgaga ttactggacc ctactgtcgc ccagtgatcg tccatcaatg
    16981 ccactttgcc gtggagcggg cggcggcggt actcaaaaac ctgaacgccg cctttaacca
    17041 gatagttata cagcaagcgc gcaccgactc tgacaatcgg catatccggt tcgccctgaa
    17101 tgatcagttt gatccgcacc ccgcgccgtg ccgctttacg caaggcgtgt aaaaatcgat
    17161 agccggggaa gaagtaggcg ttggcgataa tcacttcccg ctgcgcctga gtgagcattt
    17221 tcagataatg gcgttcaata tcatcgcgat gttcttcgtt atcgcgccag accagcaata
    17281 cctgcgcttc tcccggctgg cggttctctt ccgctttgtg atgacgtcgc caccagcgtc
    17341 gtgccgcgct ctgtctaggc aggttttcca gctcaaactg gagaatatct tcgacaatcg
    17401 gcccttcaag gcgtaccgcg taatcctgtt tagcctctgg accgtagctg gacatatgct
    17461 cggcggagta attcagcccg ccaataaagg cgatacgcgc gtcgatcacc acaattttgc
    17521 gatgcatccg gcgaaacaca ttggtgcgca taccaaaaag gcgagggcgg ggatcgtagt
    17581 agcggaacac tacgccagct gccgtcagtt cattgacaaa ctcatcgctg agatccggcg
    17641 aaccgtagcc atccagcaag acttccgctt taaccccgcg ttgcgctgct gccagtagtg
    17701 ccgcatgcag ctgtttgccg acgttatcct caaaccagat aaacgtttca agaatgatgc
    17761 gttcttgtgc ctcgccaatc gccttaaaca ccgcgggata atattgctcg ccgttttcca
    17821 gcaactggat cttattgcct tcgcgccagc tacatttcat aaatgaatct ccgcacttaa
    17881 aggggcatga tcagaaaggt gtcgccatgt ccgcagcggc aacgcggttg gcgcgctggc
    17941 gctggcattt ttgacgtaga tcctgtccag tcgtagtaga ggaaattgca ccggaaacgt
    18001 gcgcgccggg cgtccgtggg cgcgggtaaa aatctcatcc agtccggcct gcacttttaa
    18061 cggatgatta gctttttgcc gccagtcatt gaaatcaccc gccaccaata ccggttcgcc
    18121 gtccggtagc tcattcaccc attcggcgag catcgcaagc tgcgcctgac ggtgcgcctc
    18181 acgcaggccc agatgtacgc acatcacatg aatcgctttt ccggtcatcg gcggcacaat
    18241 gcggcagtag agcacgccgc gcttttccgc accatcgacc gaaacatcgc gattctcata
    18301 atgttcaatg ggataacgcg acagtacggc gttgccgtga tgcccttccg ggtatacggc
    18361 attgcgaccg taggcaaaat cgctccacat agtgtcggcg agaaactcgt agtgcgaggt
    18421 atcgggccag ttttccacat gcagcggatg aacttcgtgc gcgcccatca cttcctgcag
    18481 gcaaacaata tcggcgctga cggtacgcac ggcatcgcga agttccggca aaatgaagcg
    18541 tcggttaaac gcggtaaagc ctttgtgaat gttgatggtg agcaccttga acgaaaattg
    18601 ttgtgtttga tcgggcataa ttttcctgtc tttgcctctt atctcattga aatagtgtag
    18661 tcggcgtcac aaaaaggtgc ggtcttacgg aattttccgt aaagttcggt actctgagta
    18721 agtagagata aattcttcag gagagaagcc atgaagtggc aacaacgtgt tcgtgtcgca
    18781 acgggtctaa gttgctggca gattatgttg catttactgg tagtggcgct gctggtggtg
    18841 ggctggatga gtaagactct ggttcacgtc ggcgtgggat tatgcgcact gtattgtgtc
    18901 acggtagtga tgatgctggt gtttcagcgc caccccgagc aacgctggcg tgaggtggca
    18961 gacgtgctgg aagagctgac cacgacctgg tattttggcg cagcgctgat tgtgctgtgg
    19021 ctgttgtccc gcgtactgga aaacaacttt ttgctggcaa ttgcagggct ggcgatcctt
    19081 gctggcccgg cggtagtgtc tttgctggcg aaagataaga agttacatca ccttacgtct
    19141 aaacatcgcg tacgccgctg accctgtcgt ggccgttatc accagtagcg gccacaaact
    19201 attccacaca atatccagac tcgcatcctt caaataaatc tgcttggtaa tgtccgtaaa
    19261 gtggcgaata gggttaatcc acgtcagatt ttgcagccac actggcatgt tttccaccgg
    19321 cgaaacgtaa ccggaaagga gaatggcggg catcataaaa acaaacacgc cgataaacgc
    19381 ctgctgttgt gtcgagcaga gtgatgaaat caacagaccg aatcccacca gcgataaacc
    19441 gtaaatcacc atcgtaaagt agaacagcgc tagcgatccg gcgaagggga tttgatacgc
    19501 ccagatacca atcgccagca caatggtggc ctggaaggtg gcgacaatta acgccggtac
    19561 ggctttgccg atgaagatct gccaggtggt gagcggcgaa accagtagct gatcgagcgt
    19621 accttgttca cgttcgcggg cgacggaaag tgaagtgacg atcattacgc cgatagtggt
    19681 gatcatggcg atcagcgacg gcaccacaaa ccatttgtag tcgagattcg ggttatacca
    19741 gttgcgtacc accagctcgc tgttgttggg tttcggtttt ccttccagca gctcctgctg
    19801 ataatttttg acgatctgtt gcaggtagtt ggcggcaatt tgcgcactgt tggagttacg
    19861 cccgtcgagg atcaactgca acggcgcggt ctggaaggta tccagtttgc gcgagaagtc
    19921 agccgggaaa cgcaccagta gtaacgcctt ttgtgtgtcg atggtcgggc ggatctcctg
    19981 tgggcttttc agcagcagca catgtgtaaa ggcgctggcg cgggcaaaac gttgagtaag
    20041 ttccaccgaa tgctcgccgt tatcttcatc gtagatggcg atggtggcgt tagtcacttc
    20101 cagcgtggcg gcgaacggga acaggatcac ctgaattagc acgggtaaaa tcagaatcgc
    20161 gcgggtttgt ggttcgcgca gcaacgactg caactctttg cggattaacg tccataagcg
    20221 atgaaacatg ctcttctccc taatccagcc gacgtttggt tttcagccac gtcaggccga
    20281 taaacatcac cgccgaagcg atcaaaaaca gcacgtttac caccagcacc actggaatat
    20341 tcccggcgag gaacaggctt tgcagggtgc tgacgaaata acgagcggga ataatgtacg
    20401 tcaccgcgcg gatcaccgcg ggcatactgt cgatctgaaa aataaagccg gaaagcataa
    20461 tcgacggcag aaaagcggcg ttcagggcga cctgagcggc attgaactgg ttgcgggtaa
    20521 tcgtggaaat cagcagcccc attcccaggg tactgagtaa aaacaggctg gagataaaaa
    20581 acagaatcag cagcgaaccg cgatacggca cgccgagaat aaacactgac accagcatac
    20641 acagcaacat cgccagcatc ccgagaaagt aataagggat cagcttacac agcagcagtt
    20701 ccgtgcgggt aatctccgta gagagcagag cctccatggt gccgcgttcc cattctcgcg
    20761 ccaccaccag cgaggtgaga atcgcgccga tgaccgtcat gataatggtc accgcacccg
    20821 ggataataaa gtgctggctt atcgccgccg ggttaaacca gtagcgggtt tgtacatcaa
    20881 taagcggttc aaaagtctgc ccgttgtcct ccgctcgctg catttgccag atctgccaga
    20941 tcccttcgac atacccctgc acaaagttag cggtattcgg ctcactgccg tcggtgatca
    21001 cctgaatcgg tgcggtggcg ttggcgcgct ccatctgttc ggcgaaatcc acgggaataa
    21061 ccaccagacc gcgaattttc cccgactgca ttttggcgat cagttcctga cggttatcgc
    21121 tgatggtggc gtcgatgtag ggcgaaccgg tcatggtgtg ggtgaaatcc agcgccgctt
    21181 cgctacgctg ttccagcaaa atcccgaccc gcagcttgct ggagtcgagg ttaatgccgt
    21241 aaccaaaaat aaacagcagt agcagtggga tcactaccgc aatcagccag ctactcggat
    21301 cgcgaacgat ctgccgcgtc tctttgacgc acagcgcccg cacgcgatgc caggaaagga
    21361 tcgggttact cattgctatg ctccttatcc cagtcgtgga tcaactgaat aaaggcttgc
    21421 tccatggtgg gatcgggttg ctcatcgtta gctgactgtg ctttcaaatc gtccggcgta
    21481 ccgctggcga ttaatttccc acggtacacc aggccgatgc ggtcgcaata ttccgcttca
    21541 tccataaagt gggtggtgac catcaccgtg acgcctttct ctaccatgct gttgatgtgc
    21601 agccaaaatt cacggcgggt gagggggtca acgccggaag tcggttcgtc gagaaagaga
    21661 atatccggtt catgcatcag cgaacaggcc agcgccagcc gctgtttaaa acctaatggc
    21721 agttcatcgg tggcgtgtga ggcaatactt ttcaggccaa atgcctcgct catacgggag
    21781 attttttcgt tctgcgcccg accgcgtaag ccatacacac cagagaaaaa gcgtaaattc
    21841 tgttcgaccg tcaggttgcc gtagagcgaa aatttttgcg ccatatagcc gagatgctgg
    21901 cgcgctttac cggaactctc tttcagatcc atccccagca ccagcgcctg gccggaagtc
    21961 ggcaccagca aaccgcacat catcttaaag gtggtcgatt tacccgcgcc gtttggcccc
    22021 agcaaaccaa aaatctcccc acgtttaacg gcaaagttga cgtgatcggt ggcggcaaaa
    22081 tcaccaaatt tcttggtcag ttctttcgct tcgatcaccg tctcgccggg tgtgccttct
    22141 accgtatgca atattgcacc cagcggcgat tctgaggttc cggcaccgcc cagcaaatca
    22201 ataaacgcat cttcaaaacg cggcgtagtt tcgttgatgt tgatttccgg catcccgtcg
    22261 gcatggcgaa tatcgtctgg tgtggcctct tttttgagga tcagacgtac cgatttcccc
    22321 tgaatcatgc catcgctgac ctgcggcagt ttcaaggcgc gttgcaacag tttgcggttg
    22381 ccctcgtgtg gactggtcat cagaaagctg cgtccggcca tggtttgtgt cagggctttt
    22441 ggttctccct gatacagcaa ctcgccttcg ttcatcagta acacgtcacg gcactgctcg
    22501 gcttcgtcga gatacgaggt actccagagg attaacatcc cttcgcccgc cagctcatgc
    22561 accatctgcc acagttcgcg ccgtgagata gggtcaacgc cgacgccggg ttcatcgagc
    22621 agcaacactt tcggttcgcc caccagggta caggccaggc cgagtttttg tttcatccca
    22681 ccggagagct tgcccgccag gcgtccggta aacggcccaa gagacgtaaa ctccagcagg
    22741 cgagcaaaag tttgcttacg tgcctcgccg gtgacgctgc gcaaatccgc gtacagattg
    22801 aggttctcca tcaccgtgag atcttcatac agaccaaatt tctgcggcat atagccgagc
    22861 acggcgtgca gcgcgccgtc gtttttgatc ggatcaaagc caatcaccgt ggcactgccg
    22921 ctgtcgggtt tcagtaatcc cgccaacatc cgcatcagtg tggttttacc tgcaccgtcc
    22981 ggccccacca accccgtcac ataaccggcg tgaatggtac aatcgagcgg cgcgacggcg
    23041 ggcttgtcca tgcccggaaa gcgtttttcc aggccgttca gcgtgataac ggcatcattc
    23101 atgtcctgcc tcgtcaccga attgtaccgt cactggcatt ccctggcgta acgcatcatc
    23161 ggcgtcggtc accacaatac gcaggcgata gacgaggtcg gtacgcagat ccggtgtttc
    23221 gacggttttc ggggtaaatt cagcagtcgg cgaaacgaaa ccaatctgcc cgtgatacgg
    23281 cttgtccggg cgaccatcgg tataaagcag cactttgcgc cccggctggg cctggtcaag
    23341 attccgttca tcaacataag cgcgcaccca caccggacgc gttagtgaaa cggtaaacac
    23401 cgtgccacct tcattgagaa ccgtgcctgg ctctaccgcg cgcgttaaca gcgtgccatc
    23461 agacggtgct atcaacgttg agtcctgtaa attcagctcc gcctgcgcca gttgcgcctg
    23521 cgcctgttcg aggctggctt tcgcctgagc gatgtcctgt tcacggttac cggaacggta
    23581 ctgacgcaat ttatcctgtg ctgatttcag cgttgcctgt gcctggtcgc gcgaggagcg
    23641 ggcattttcc aggtcatttg ccgaaatggt gcggcttttc cacagtcctt gctggcggtt
    23701 atagaagttc tgcgcatagt cataggcggc ttgcgcctgt ttcaccgctg cggcggcctg
    23761 agcgatttct tcatcgcgat acccggcaag catcagatca tactgcgcct gtgccaccga
    23821 gacgcccgct ttcgcctgca tcagggcaat ctcatacggc ttgtgatcca gttcgcccag
    23881 cacctggccc gctttgatag catcaccttc gtccaccgcc agcgattcaa cgcgcccccc
    23941 aacacggaaa ctaagattca ccgtacgaat atccacgttg ccatacagcg tcaggccgtt
    24001 atcctggcgg ctttgatacc accagtagcc tccggcaacc acggcggcaa gtaccactac
    24061 cgccagtccg atcacgacag gttttttcat cactacaaac tcctttgcga taatccttgc
    24121 aaaatcaggt cgatatgaca ggtcaccgtc tggttgatca gttcggtctt ttcttcatcg
    24181 aacgcggtcc agccggtacg taacagaatt gtttctttgc caagacggaa cgccagaatc
    24241 tcaccaatca acgcatgggt atgaaggatc atgcgggtgt cattagcgtc gcagccggtc
    24301 caggcggcaa tcaggcgtgt caggtggctg tgtagcggac taatcacctg ttcgtgcacc
    24361 aggtggtagg ctgccgtggg agagagctgc tcacgggaga taaacttgct gaggttgacg
    24421 gtgtcatcct gggtgagcag cttaatcatg ttcctgcagg cgcgaaggat cagttcacgg
    24481 atggcagccc gatcaggctg tggttgggcg aacaagcgtt cggcttcctc ggcatgcgga
    24541 cggaactgct cgccaataaa atcggcaatc cactgggcgc aggcgaggta caaatcttct
    24601 ttcgaaccga agtagtaggt gatggcagcg atattctgcc cggcctgggc ggctatctcg
    24661 cgggtggtgg cgttcattcc atattcacca aactgcgcca gtgcggcagc aatcagctgt
    24721 tttttcgcct gttcaccctt gattgtcatg gcaggattat tcatcgcaca gtccattctt
    24781 aatcaaatga ttgattaaga ttatgactcc atatgggagt tgtccagtat ggctaagaat
    24841 tttagctacg ccagtcacag ggataattta tgcgctgcgt cacaaaaact gctacactcc
    24901 gctccctcat gacattgtgg tttttgtcat tttccttttc agtatctccc tgaaaactac
    24961 accggtaacg gtcggggcgg ttcggagtag ttatgtcttt cgattctttg ggtttaagcc
    25021 ctgatatcct gcgcgccgtt gccgagcagg gttaccgtga acccacccct attcagcagc
    25081 aggcgatccc tgctgtgctg gaaggccgcg acctgatggc tagcgcccag accggcaccg
    25141 gcaaaacagc gggctttacg ctgccgctgt tgcaacacct gatcactcgc cagccgcacg
    25201 ccaaagggcg tcgtccggta cgtgcgctca ttcttacccc gacccgtgaa ctggcggcac
    25261 agattggcga aaacgtccgt gattacagca aatacctgaa cattcgttcg ctggtggtgt
    25321 ttggcggtgt cagcattaac ccacaaatga tgaagttgcg cggcggcgtt gatgtgctgg
    25381 tggcaacgcc gggacgtttg ctggacctgg aacatcaaaa tgcagtgaag ctggatcagg
    25441 ttgaaatcct cgtcctcgat gaagctgacc gcatgctcga catgggcttt atccacgata
    25501 tccgtcgcgt gttaacaaaa ctgccagcga aacgccagaa cctgctgttc tctgcgacct
    25561 tctctgacga tattaaagcc ctggcggaaa aactgttgca caacccgctg gaaatcgaag
    25621 tggcacgccg caataccgcg tctgatcagg tgacccagca cgttcacttt gtcgataaga
    25681 aacgcaaacg cgaattgctg tcgcacatga ttggtaaagg gaactggcag caggtgctgg
    25741 tgtttacccg taccaaacac ggcgctaacc atctggctga gcagctcaat aaagatggca
    25801 tccgtagtgt ggcgatccac gggaataaat cgcaaggtgc gcgtacccgt gcgctggctg
    25861 attttaaatc gggtgacatt cgcgtactgg tggcgaccga tatcgctgcg cgcggtctgg
    25921 atatcgaaga gttgccgcac gtcgtcaact atgaactgcc aaacgtaccg gaagattatg
    25981 tccaccgtat cgggcgtacc ggtcgtgcgg ctgctaccgg tgaagcgttg tcgctggtgt
    26041 gtgttgatga acacaaactg ctgcgtgata tcgaaaaact gctgaaaaaa gagatcccgc
    26101 gcattgcgat tccgggctat gagccggacc cgtcaatcaa agccgaaccg atccagaacg
    26161 gtcgccagca acgtggcggc atcaggctga tgaacaaacg caaaactgcc tgatgcgcta
    26221 cgcttatcag gcctacgtga actctgcaat atattgaatt tgcatgcttt tgtaggccgg
    26281 ataaggcgtt tacgccgcat ccggcatttc acaacaagca cttgtcagca atttgagaac
    26341 acgggaaaaa atttatctgt tttaccgccc ataagccacc gagacgttac ttctctatag
    26401 ccaattgttc gcgcaactcc attaaaaggt agcccagtcg attcttgccc ttaccattac
    26461 caccgtctcc ccagtaagca tcgttttccg tatgctcaac cagttttgcg ggcgcggttg
    26521 ccagcaagag cacacgcagt tctgcatgct gttcgaattt agcgcgaagt gctttacgca
    26581 tcacctgttc tttaaccgac tcccagtttt tacgaagtgg cttggaacga ttacgcccca
    26641 tgcgagcggc gaccatgggg gaagaaaccc gacggatctc ttcgcggtat ttttcatcaa
    26701 ggaatttttg tgcctgaaaa tagtgttcgg aggtgggcca ggtattcccg tcaaccttga
    26761 tgggcctggc ggcaaagtta gaaaaatcac cgtagtcatc gctggtgctg tagaaattta
    26821 tgatggtgtc ttgcatgacg tgttggatcc tttgtgctcg aacgggcatt aaaccgcatt
    26881 atgttggtgg ttattgcgag ccgctttcca gaaacagaaa aaccattacc cctgaaaacc
    26941 gaaaaatgcc acaatattgg ctgtttatac agtatttcag gttttctcat ggcattaacc
    27001 gccgcgctta aagcgcaaat tgccgcctgg tataaggcgc ttcaggaaca gatccccgac
    27061 tttattcccc gtgcgccgca gcggcagatg attgcggacg tcgccaaaac gctggccgga
    27121 gaagaagggc ggcatctggc gattgaagcc cccaccggcg ttgggaaaac gctctcctat
    27181 ttgatccccg gcatcgccat tgcccgcgaa gagcaaaaaa cgctggtggt gagtaccgcc
    27241 aacgtggcgt tgcaggatca gatttacagc aaagatttac cgctgctgaa aaagatcatt
    27301 cccgatctta aattcactgc cgctttcggg cgtgggcgct acgtttgtcc acgtaatctg
    27361 acggcgctcg ccagcactga acccacgcaa caggatctgc tggcgtttct tgacgacgaa
    27421 ctgacgccaa ataatcagga agagcaaaaa cgttgtgcga agctgaaggg cgatctcgac
    27481 acttataaat gggatggtct gcgcgatcat acggatatcg ctatagatga tgatctctgg
    27541 cgtcgtttga gtaccgacaa agccagctgc ctcaaccgta actgttacta ctatcgtgaa
    27601 tgcccgtttt ttgttactcg tcgggagatt caggaagcgg aagtggtggt ggcaaaccat
    27661 gcgctggtga tggcggcgat ggaaagcgaa gccgtactgc ctgacccgaa aaatctgctg
    27721 ctggtgctgg acgaaggtca tcacctgcca gatgtggccc gggatgcgct ggagatgagc
    27781 gctgaaatca ccgtgccctg gtatcgacta cagctggact tgttcacgaa actggtcgct
    27841 acctgcatgg agcagtttcg cccgaagacc atcccaccgc tggcgatccc tgaacgtttg
    27901 aatgcgcatt gtgaagagtt gtatgagctt atcgcctcgt taaacaacat tctcaatctc
    27961 tacatgcctg ccgggcagga ggcagagcac cgttttgcga tgggcgaact gcctgatgaa
    28021 ttgctggaga tctgccagcg gctggcaaaa ctcaccgaga tgctgcgtgg tctggcggag
    28081 ttatttctta acgatttaag tgagaaaacc ggcagccacg atattgtgcg tctgcatcgg
    28141 ttgattttac agatgaaccg cgcgctgggg atgtttgagg tgcaaagcaa actctggcgg
    28201 ctggcgtctc tggcgcaatc ttccggtgcg ccggtgacca aatgggcgac gcgggaagag
    28261 cgcgaagggc agctacacct ctggtttcac tgcgtgggaa tacgtgtcag cgatcagctg
    28321 gaaaggctgc tgtggcgcag tattccgcac attattgtca cctccgcaac cttgcgttcg
    28381 ctgaatagtt tttcgcgttt gcaggagatg agtggtctga aagagaaagc gggcgaccgt
    28441 tttgtggcgc tggattcccc ctttaaccac tgcgaacagg gcaaaattgt tattccccgg
    28501 atgcgctttg agccttccat cgacaacgaa gagcaacaca ttgccgaaat ggcggccttt
    28561 ttccgtgaac aggtggagag caaaaaatat ctcggtatgt tggtactgtt tgccagcgga
    28621 cgggcgatgc agcgctttct cgactatgtg acggatttac gcctgatgtt gctggtgcag
    28681 ggcgatcagc cgcgttatcg cttagttgaa ctgcaccgca aacgcgttgc caacggtgag
    28741 cgtagcgtgc tggtgggctt acagtcattt gccgaagggc ttgatttgaa aggtgatctg
    28801 ctcagccagg tgcatatcca caaaatcgct tttccgccca tcgacagccc ggtggtgatc
    28861 accgaagggg aatggctgaa aagcctcaac cgctatccgt ttgaggtgca aagcctgccg
    28921 agcgcctcgt ttaacctgat tcagcaggtt gggcgactga ttcgaagcca cggttgctgg
    28981 ggcgaagtgg ttatctacga caaacgcttg ctgacaaaaa attatggcaa gcgactactg
    29041 gatgcattac cggtatttcc gatagagcaa ccggaagtcc ctgaaggtat agttaaaaag
    29101 aaagaaaaaa cgaaatcccc acgccgtcgg cggcgttaat gatgtgagtc aggtaaggag
    29161 tcgtaaatgg actatcgcaa aatcattaaa gagatcgggc gcgggaaaaa ccacgcgcgc
    29221 gatttagacc gggatactgc gcgcggtctg tatgctcata tgctcaatgg tgaagtccct
    29281 gaccttgagt tgggcggcgt attgattgcg ctgcgtatca aaggggaagg ggaagcagag
    29341 atgctcggct tttacgaagc catgcaaaat cacaccatca agctgacgcc gcctgcaggc
    29401 aagccgatgc cgattgtcat ccccagttac aacggcgcgc gtaaacaggc taacctgacg
    29461 ccattattgg cgattctgct gcataaactc ggttttcctg tggtggttca cggagttagc
    29521 gaagatccaa ctcgcgtgct gacagaaacc attttcgaac tgatgggtat tacaccaacg
    29581 ttgcacggcg ggcaggcgca ggcgaagctc gacgaacatc aaccggtgtt tatgcctgtc
    29641 ggcgcgtttt gcccgccgct ggaaaaacaa ctggcgatgc gctggcggat gggcgtgcgt
    29701 aacagtgcac ataccctggc gaaactggcg acgccatttg ccgaaggtga ggcactgtgt
    29761 ctttccagcg tttcgcatcc ggaatacatt ggacgcgtcg cgaagttctt tagcgatatc
    29821 ggcgggtggg cgttactaat gcatggtact gaaggcgaag tgtatgctaa tccgcagcgc
    29881 tgcccgcaga tcaatctcat tgaccgtgaa gggatgcggg tgctgtatga aaaacaggac
    29941 cctgctggta gcgagttact gccacaagca aaagatccgg aaaccacggc gcagtggact
    30001 gagcgttgcc tggctggcag cgaagcgatt cccgaatcgc tgaaaatcca gatggcttgc
    30061 tgcctggtgg ctacgggtga agcggcaact atcagcgatg gcctggcgcg cgttaatcag
    30121 gcattttaat ttttatctca gccggatgag ccacgtcatc cggcttttac cccaccgcaa
    30181 tatgaaattc ctgcatcttt attgaccttc ccgcgcccgg cgtgcagcat aaaaaaacaa
    30241 caaacacata acataaacag gagttaacca tggaaagtgg tcatcgcttt gatgctcaga
    30301 cgctgcacag ttttattcag gctgtatttc gtcagatggg tagcgaggaa caagaagcga
    30361 aattagttgc cgatcattta atcgcggcaa acctggcagg gcatgattct catggtattg
    30421 gcatgatccc aagctatgtg cgttcctgga gtcaggggca tctgcaaatt aaccatcatg
    30481 ccaaaatcgt taaagaggcg ggggcggcag tcacgctcga tggcgatcgc gcattcggtc
    30541 aggtcgcggc acacgaagcg atggcgctgg ggattgagaa agcgcatcag cacggtattg
    30601 ctgccgtggc gctacataac tcgcatcata tcggccgtat cggttactgg gcggagcagt
    30661 gttcagcggc ggggtttgtc tctatccact ttgttagcgt ggtcggtatt ccaatggtcg
    30721 cgccgttcca cggtcgcgac agccgctttg gcaccaatcc cttctgtgtg gttttccctc
    30781 gtaaagataa tttcccgctg ttgcttgatt acgccaccag cgccattgca tttggcaaaa
    30841 cccgcgtcgc ctggcataaa ggcgtccccg tgccgccagg ttgcctgatt gacgttaacg
    30901 gcgtgccgac gaccaatccg gcggtaatgc aggagtcgcc gttggggtcg ctgttgacct
    30961 ttgccgaaca taaaggctac gcccttgcag cgatgtgtga aattcttggc ggggcgcttt
    31021 ctggcggtaa aacgacgcat caggaaacgt tgcaaaccag ccccgatgcc attcttaact
    31081 gcatgaccac tatcatcatc aacccggaac tgttcggcgc gccggattgt aacgcgcaga
    31141 ccgaagcctt tgccgagtgg gtgaaagcct cgccgcatga tgacgataag ccgattttgc
    31201 taccgggcga gtgggaagtg aacacgcgtc gcgaacggca ggagcagggg attccattgg
    31261 atgcgggaag ctggcaggcc atttgtgatg cggcgcggca gattggtatg tcggaagaga
    31321 cgttgcaggc tttctgtcag cagttagcca gctaaaaaaa agcccgtcca gtggcggacg
    31381 ggcaaacaag ggtaacatag gatcaatgag ggttagagca tatgcgtctg tcggcaaaca
    31441 gacagggaaa tacttgtgct ggacgtagcg taaacgcctg atccgtccaa cggttcggat
    31501 tttgtaggcc tgataagacg cggtagcgtc gcatcaggca tctgttgccg gatgcggcat
    31561 aaacgcctta tccgtcctac gaattactta taaattaccg cagtaccgct taatttattg
    31621 ttgttggtgg cggaagtgat gctgtatcca ctggcaccgg ctgccgccgc tttctcagcc
    31681 agtttcgctt ccagggcatc gagggtggat gcgccatcgg cagaaaccac gccgatttta
    31741 ttcatgttct gtgcctggga tgccgttacc ggttccgccg cgaacacgcc aaatgacaga
    31801 gttgaaagag ccatagcagc aacaacagta ttgatagttt tcataattaa tctctcgcag
    31861 gtgatttttg tataagtgac gttgtttcgt cgatgtgatg agtatcacgt ttttttacga
    31921 gagataaaat cgaagagaat tgaccgcctt gttcaaataa attgattgat atctaacata
    31981 ctgataaacc atttatttgt tgtacttatg gacttgtttt atgccgcgtg gcagtcacag
    32041 cagcgttaaa ggcacggaat gccacgcaaa atacaaatta tactcgctat gcatctcgcc
    32101 agttgtagtt aacgtaactg tctgtctttc gaacctctgc gattatatcc tgtatacgct
    32161 ggtttttgta aatctttctc ctgctgacaa tgaatacata agcgcacgct aggaatggct
    32221 tcccgacggg cttgcgggat gggggcaccg cactcttcac attcatacag gctttcgcca
    32281 cgcggaattt caccccgagc gcgggcaatc gcatcttcaa ttgtactgtt gatctgttcg
    32341 ttgacggcgt catcgttagc ccaagcggat gccatatcgt cctccccata tcaatgcttg
    32401 tacagttaag tgtagctaat atagggacga acctgggcag ttcaagcgtc agatctccga
    32461 ccattcccgc agcagattat gataaagatt gagcagcgac aggatctctt cattttcacc
    32521 atagcggctt ttcagcgact gaatattttt gtccagttca aacagcatgg cgcgcttttt
    32581 atcatcgcgg atcatcgact ggatccacat aaatgatgcc actcgtacgc cgcgggttac
    32641 gggtgtcacg caatgcaggc tgctggaggg atacaacacc agatcacctg ccgggagttt
    32701 tacccgatgt tgtccaaagg tgtcattaac gaccagttcg ccgccgtcgt agctttgtgg
    32761 atcgcttaaa aacagcgtgg cagaaaggtc agtgcgcatc cagccgtttt gtggatggct
    32821 gcgtactgcg ccatccacat ggaaaccata ggtttcattg ttctgatagc gattaaacag
    32881 cggcgtggaa agggtacgcg gcaaggcagc ggcaaagaac aatgcatgtt ggttaaccgc
    32941 gttcagcacc tcattttgca gtgcggcgta taacgcgctg cgggtgtcta cttgctgatt
    33001 gttcttaact tgtgcacctt gtgctccggt ggtgacgcgt ccatccaccc attcggcttg
    33061 ttccagttgt tcgcgaaaat gggcgacgtc ctgtggcgat aacacgccgg gaatgtggta
    33121 catcatacaa gttctccaaa aagtggggcc tgcgccccac atctgaatca gaaatgcata
    33181 ttggctgtga gcaagaaggt tcttggctcg cccgggtgat aacggtagcc gctcttgttg
    33241 attgaggcga cgtaatcggt atcaaacagg ttgtaaacgt ttagctggaa gtcgagattg
    33301 cgattaaccc gataccccag tttggcatcg gcgacccagt aaccttctgt aaatgctggc
    33361 gttcccaccg cgccgtctga acctttatgt atactgccga tatagcgtgc gcccgcgcca
    33421 acagagatat catcggttgc ctgatattgg ctccataagg tgaaggcgtg ctccggggta
    33481 tacggcagcg atgaggaacc atcctgggca acatctttgc cgtttttgat ggttgctttt
    33541 tgctgggtat agccgccaat cacctgccac gcgggagtga tattccctgc cacggatatc
    33601 tcatagcctt cgacgcgttt cttaccgtat tgcgagtaag tcccgtcatc attttgctca
    33661 acttcatttt cgatatcagt acggaacagc gcggcggtga gcaacaggcg tttatccaga
    33721 acctgccatt tggtgccaat ctcgctggtg ttggcttttt gcggtttaaa atcggtgcgg
    33781 ttggcactgt taccactgcc agactgcgca agggcgaagt tgttgccgcc cggaggctgc
    33841 tgggaaacgg catagttaat atagacattg ccgttttccg tcaggtggta cagcgccccg
    33901 gctttccagt tcaccagatt gcccgacttg gcggtgtcga cggtggtgac tggagaacct
    33961 tttgccacac cagctgggca ggtgatggca ccgcgtccgc tgccgccgca ggcggtggca
    34021 ctgtcatatt cagtatgata attatccaga cggatcccgc cgttcagctc aaaatcacgg
    34081 gtgatttgca gcgtatcgaa ggcgtaaatt gcgaaggtat ccgtctgacc attggcgttt
    34141 gcgccgttgc gcgtcaggcc gccgggatga attctgctgt caggatgata aatatttacc
    34201 gccggtaacg tcaccggatt aacgccatag ttagtctgcg tttcacgggt aaattccacg
    34261 ccggtactga catcatgacc gatagaaccg gtatagaacg tcgaggtcag gttggtctgg
    34321 ttggtgagaa ttttattact cacatctttg gtattcgccg tgcgtgacta ggtccagcta
    34381 ttcacatcgc tggtgggctg agtaatattc gacgccccgc ccataatcgc cgtcatcagg
    34441 taatcctgct ttacgcgcga ccaacgggta gtattgcgaa tggtggtgtt atcgttgatg
    34501 tcgtgctcaa aacgcatggt ggcggtgtcg gtggtcgaat cgtcgtaatc ggaatccgtg
    34561 ccgtaaaagt tatgagtatc aacttttccg gaatgattca gggccgccgc tcccgcagat
    34621 ggggcagaat agcccggcaa accgatggtc ggaatgccgc cgtctggcgt gttgtgctga
    34681 gtgacatgca gataattaag atacaaacga tttgctgtac caaggccaaa agcgacagaa
    34741 ggggcgacgc cgtaacgctc atttttgact ttgtcgcgtc cggcatcgtg ggttttttcg
    34801 cccatcacat tcaggcgcac cgcggtagta tcaccaatga cctgattgac gtccagcgtg
    34861 ccgcggcgga accaggcgct gccaatactg gcggaggcgt caatgccgga atcattgcgc
    34921 ggctgcttgc tgatcatatt gatcgagcct gtcggtgcgc tgcgcccgta gtcggtgccg
    34981 gacggccctt taatcacttc gacctgctcg gtattgaagg tgtcgcgcga gacgctgccg
    35041 atatcgcgaa taccatcaat ataaatactg ttagaggtat cggcaccacg catataaatg
    35101 gcgtcgccag tggtggagtt accgttctca cccgcaaaaa acgcacccac gccggggtaa
    35161 tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc cggaaacgct
    35221 cacttgtttc cggatttttt tatgcacatt ggacaggctc ttgatctggt atcccgttac
    35281 gattctctgc gtaacccact gacttctctg gg
//
LOCUS       DAZSNO010000026        34903 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZSNO010000026 DAZSNO010000000
VERSION     DAZSNO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34903
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.026"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(587..730)
                     /gene="ypaB"
                     /locus_tag="ACHWN2_001246"
     CDS             complement(587..730)
                     /gene="ypaB"
                     /locus_tag="ACHWN2_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR8930860.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            858..3143
                     /gene="nrdA"
                     /locus_tag="ACHWN2_001247"
     CDS             858..3143
                     /gene="nrdA"
                     /locus_tag="ACHWN2_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR8930861.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3377..4507
                     /gene="nrdB"
                     /locus_tag="ACHWN2_001248"
     CDS             3377..4507
                     /gene="nrdB"
                     /locus_tag="ACHWN2_001248"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR8930862.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4507..4761
                     /gene="yfaE"
                     /locus_tag="ACHWN2_001249"
     CDS             4507..4761
                     /gene="yfaE"
                     /locus_tag="ACHWN2_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR8930863.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="ACHWN2_001250"
     CDS             complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="ACHWN2_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFR8930864.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5545..5649)
                     /locus_tag="ACHWN2_001251"
                     /pseudo
     CDS             complement(5545..5649)
                     /locus_tag="ACHWN2_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5644..5886
                     /locus_tag="ACHWN2_001252"
                     /pseudo
     CDS             5644..5886
                     /locus_tag="ACHWN2_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="ACHWN2_001253"
     CDS             complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="ACHWN2_001253"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR8930865.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="ACHWN2_001254"
     CDS             complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="ACHWN2_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR8930866.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8640..10268
                     /gene="glpA"
                     /locus_tag="ACHWN2_001255"
     CDS             8640..10268
                     /gene="glpA"
                     /locus_tag="ACHWN2_001255"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR8930867.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10258..11517
                     /gene="glpB"
                     /locus_tag="ACHWN2_001256"
     CDS             10258..11517
                     /gene="glpB"
                     /locus_tag="ACHWN2_001256"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR8930868.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11514..12704
                     /gene="glpC"
                     /locus_tag="ACHWN2_001257"
     CDS             11514..12704
                     /gene="glpC"
                     /locus_tag="ACHWN2_001257"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR8930869.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12897..13847
                     /locus_tag="ACHWN2_001258"
     CDS             12897..13847
                     /locus_tag="ACHWN2_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR8930870.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13888..14349)
                     /locus_tag="ACHWN2_001259"
                     /pseudo
     CDS             complement(13888..14349)
                     /locus_tag="ACHWN2_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(14413..15615)
                     /locus_tag="ACHWN2_001260"
     CDS             complement(14413..15615)
                     /locus_tag="ACHWN2_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR8930871.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15702..16244)
                     /locus_tag="ACHWN2_001261"
     CDS             complement(15702..16244)
                     /locus_tag="ACHWN2_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR8930872.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16523..16948
                     /gene="nudI"
                     /locus_tag="ACHWN2_001262"
     CDS             16523..16948
                     /gene="nudI"
                     /locus_tag="ACHWN2_001262"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR8930873.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16987..17589)
                     /gene="ais"
                     /locus_tag="ACHWN2_001263"
     CDS             complement(16987..17589)
                     /gene="ais"
                     /locus_tag="ACHWN2_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR8930874.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQNKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17897..19036
                     /gene="arnB"
                     /locus_tag="ACHWN2_001264"
     CDS             17897..19036
                     /gene="arnB"
                     /locus_tag="ACHWN2_001264"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR8930875.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19040..20008
                     /gene="arnC"
                     /locus_tag="ACHWN2_001265"
     CDS             19040..20008
                     /gene="arnC"
                     /locus_tag="ACHWN2_001265"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR8930876.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20008..21989
                     /gene="arnA"
                     /locus_tag="ACHWN2_001266"
                     /pseudo
     CDS             20008..21989
                     /gene="arnA"
                     /locus_tag="ACHWN2_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21986..22876
                     /gene="arnD"
                     /locus_tag="ACHWN2_001267"
     CDS             21986..22876
                     /gene="arnD"
                     /locus_tag="ACHWN2_001267"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR8930877.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22876..24528
                     /gene="arnT"
                     /locus_tag="ACHWN2_001268"
     CDS             22876..24528
                     /gene="arnT"
                     /locus_tag="ACHWN2_001268"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR8930878.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24525..24860
                     /gene="arnE"
                     /locus_tag="ACHWN2_001269"
     CDS             24525..24860
                     /gene="arnE"
                     /locus_tag="ACHWN2_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR8930879.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24860..25246
                     /gene="arnF"
                     /locus_tag="ACHWN2_001270"
     CDS             24860..25246
                     /gene="arnF"
                     /locus_tag="ACHWN2_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR8930880.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="ACHWN2_001271"
     CDS             complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="ACHWN2_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR8930881.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25617..26972)
                     /gene="menE"
                     /locus_tag="ACHWN2_001272"
     CDS             complement(25617..26972)
                     /gene="menE"
                     /locus_tag="ACHWN2_001272"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR8930882.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26969..27931)
                     /gene="menC"
                     /locus_tag="ACHWN2_001273"
     CDS             complement(26969..27931)
                     /gene="menC"
                     /locus_tag="ACHWN2_001273"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR8930883.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27931..28788)
                     /gene="menB"
                     /locus_tag="ACHWN2_001274"
     CDS             complement(27931..28788)
                     /gene="menB"
                     /locus_tag="ACHWN2_001274"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR8930884.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28803..29561)
                     /gene="menH"
                     /locus_tag="ACHWN2_001275"
     CDS             complement(28803..29561)
                     /gene="menH"
                     /locus_tag="ACHWN2_001275"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR8930885.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29558..31228)
                     /gene="menD"
                     /locus_tag="ACHWN2_001276"
     CDS             complement(29558..31228)
                     /gene="menD"
                     /locus_tag="ACHWN2_001276"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR8930886.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31317..32612)
                     /gene="menF"
                     /locus_tag="ACHWN2_001277"
     CDS             complement(31317..32612)
                     /gene="menF"
                     /locus_tag="ACHWN2_001277"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR8930887.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="ACHWN2_001278"
     CDS             complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="ACHWN2_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR8930888.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33051..33512)
                     /locus_tag="ACHWN2_001279"
     CDS             complement(33051..33512)
                     /locus_tag="ACHWN2_001279"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8930889.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33577..34494
                     /gene="rbn"
                     /locus_tag="ACHWN2_001280"
     CDS             33577..34494
                     /gene="rbn"
                     /locus_tag="ACHWN2_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR8930890.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34685..34873
                     /locus_tag="ACHWN2_001281"
                     /pseudo
     CDS             34685..34873
                     /locus_tag="ACHWN2_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8536 a         9339 c         8648 g         8380 t
ORIGIN      
        1 cgtaatgcct gaaaggcttt gcctgctggc gtgacaactc gctttgatat catatccctg
       61 gcataaatca atggccgccg cgacaccgtt agcagagaaa agagatgcaa tcattgacgg
      121 gagtagagat aaatactcct tgcgtagaaa gataatccgc atattaatct gaccatcatt
      181 ttatataaga cggcatattt aaacgcatta tacaatatgg gttatctttc ttttgtaaat
      241 tgtccaaccc gatgcaatgg ctattttgtt attatttgat gaataatatc agtgcgtcat
      301 aattcaagtt aataacctta agggatacca gttatattta aactaaatta aagtaatgaa
      361 taattttctt ataatataag gtaaattaac aaaatggctt cgcatttaac aataaccgaa
      421 tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat tgaccacaac
      481 tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa
      541 agattaataa accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg
      601 aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac
      661 ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca
      721 agagggtcat tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt
      781 tctaagcagc ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa
      841 aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag
      901 agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata
      961 acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga
     1021 cctctgacat ccacgaaact attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc
     1081 cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg
     1141 gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat
     1201 acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta
     1261 tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat
     1321 atctagtaca gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc
     1381 ttgttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc
     1441 gtttttatga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg
     1501 tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg
     1561 attccatcaa cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg
     1621 gcatcaacgc cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc
     1681 ataccggctg tatcccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg
     1741 gcggtgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa
     1801 gcctgctggt gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact
     1861 acggggtaca aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc
     1921 tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt
     1981 ttgaacgcct gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag
     2041 ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc
     2101 agaacgttga ccactgtaat acccatagtc cgtttgatcc gaccatcgcg ccagttcgcc
     2161 agtctaacct gtgcctagaa attgccctgc cgaccaaacc gctgaacgac gtcaacgacg
     2221 agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc
     2281 tggatgaact ggaagagctg gcgattctgg cggttcgtgc acttgacgcg ctactggatt
     2341 atcaggatta cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt acgctgggta
     2401 ttggtgtgat taacttcgct tactatctgg cgaagcacgg taaacgctac tccgacggca
     2461 gcgccaacaa cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt
     2521 ctaatgagct ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga
     2581 aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc
     2641 tgcattacga ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca
     2701 cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta
     2761 ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg
     2821 tggtgccgga ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta
     2881 acgatggcta cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg
     2941 ctaacaccaa ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc
     3001 tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc
     3061 gtgacggcgc tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg
     3121 aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg
     3181 gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg
     3241 ccggatgcag cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac
     3301 gcgccagcgt cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc
     3361 ccaacaggac acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa
     3421 agaaccgatg ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga
     3481 catcttcgaa aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga
     3541 cgtctcccgc gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat
     3601 cagcaacctg aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc
     3661 gctattgccg cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc
     3721 agaaacgatt cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc
     3781 tgttgtgttt gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc
     3841 cagctattac gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac
     3901 ccacaccgtt aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta
     3961 tctctgcctg atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg
     4021 ttcctttgcc tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat
     4081 tgcccgcgat gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag
     4141 cggcgcggat gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga
     4201 cctgtttgtc caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg
     4261 ttcgatgatt ggtctgaata aagatattct ctgccagtac gttgaataca tcaccaatat
     4321 ccgcatgcag gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg
     4381 gatcaacacc tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag
     4441 ttcttatctg gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca
     4501 gctctgatgg cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa
     4561 cacccttccc ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgccgc
     4621 gaaggttact gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc
     4681 gaaccgttag cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc
     4741 gatattgaaa tcgagttgtg atcgggtact agcgccagat acgacgctta cgcgtattat
     4801 ctggcctaca acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgcttta
     4861 gggatcggct ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga
     4921 cgcaagcggc gacgactttt ctccagatca agaaaaccag cttctgcctt accttctgtt
     4981 ttcacataga tatggcgaac atagcaacag ccatgctgac gattaacgct atgcattttc
     5041 ttaaacgcca gtgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat
     5101 actgcatgcc gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc
     5161 agcaacgcgc gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg
     5221 atgacaccgg cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg
     5281 ccgaacggat aacgtactga atgaaacaga tgatgcgtca tgcgctttac atacagcttt
     5341 ttgccgttgc gctcgacgca ttgcaccccg ctcataccat tacggcgata gttaggctct
     5401 tcaacccagt cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact
     5461 gccataccct atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagg
     5521 aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt gccgctacag gtgtgacacc
     5581 gcttttgccc ttcatttgca gcgcatagat tccaccagcc actaccacta atagtgcgcc
     5641 cagcagataa aatgtgaatt taatcagtcg gatacagcga tgaattttat tcgacaggga
     5701 ttaggcatcg ccttacaacc agagttaacg ctgaaaagca ttgcaggtga attgtgttcc
     5761 gttcctcacg aaccaacctt ctatcgacag atttcgttgc tgactaaaga aaagccggta
     5821 gaaggcagtc cactgttttt actacaaatg tgcatggaac aattagtggc gattggaaaa
     5881 atttgatatg agacaggatg gcgcatgaac gccatcctgc agtaacatta ctctttatta
     5941 agaaatttta ctgccttatc agggaaatca gtaaacagcc catttacacc cgctttgtta
     6001 tacagagcat catataactg attcacatca ggagtgtatt caggcagttt atctgaccgc
     6061 acggtataag gatgcactac cagtttattc tgctgggcat cttgcaccat gccagtgagt
     6121 ttgatattac ccggctgcga tgtctcctca atcaacatat ggtaatccgg accaatacca
     6181 tctgcatatt ccgccacctg tttcatggct cccggcttaa acatccagtc gtagttgtaa
     6241 ttaacccagc ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc
     6301 agctgtacca gattgagctc catgcccatt ttgggttcca gctcattctt aatacgcttc
     6361 agctcatcag catcaaaaca ttgcaaataa actttatcgt ctttaccggt gtaaccatat
     6421 ttcttcagca cttccagcgt ttttgccgca atatccttcc cttcctgatg atggaaccac
     6481 ggcgctttga tttctggata gataccgata tttttcccgg tagagtgatt taacccctga
     6541 acaaattcaa tctcttcttc aaaggtgtgc acccggaagt cggacttacc cattgggaaa
     6601 cgccccggat aagtctgtac ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc
     6661 agcgacttaa tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc
     6721 cgatccggga aacgatcggc aacatcagta acacgatcga gataatggtc atgcagaaca
     6781 accagatggt cgtctttggt catcaccaaa tcctgttcca gataatccgc tccctgcgca
     6841 tacgccatcg cttttgctgg cagcgtatgc tccggcaaat atccactggc accgcgatgg
     6901 gcgatgacta ttttttcgtt gctgtccgcc gccattgcac tgcttcccat gactatagtg
     6961 ctcatcatga tcgccatgct aaggtttttc agcttcaatt tcatgccatt agcctccgtt
     7021 gcgtttttgc agtaattgtt catggcgacg tttttcgcca atcatcacaa caatcaacaa
     7081 gataaccgcc agaatgctgc cgccaatcat taccataaag ccgccatccc agccgaagaa
     7141 gtccacggtg tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag
     7201 cccggtaaag cccgctgccg tacctgccgc ttttttcggt gccagttcca gcgcatgcag
     7261 accgatcagc atcacaggac cgtagatcag gaagccgata acaatcatac aaatcatatc
     7321 gacggttggg ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt
     7381 cataaagaag acgccggttg ccccacggtt accacggaag actttgtccg acatccagcc
     7441 gcacagcagg gtgcccggaa tacccgcata ctcatagaag aagtaggccc aggaggattt
     7501 atccagcgcg aaatgcttaa cctctttcag ataagtcggt gaccagtcga ggatgccgta
     7561 acgcagcaga taaacgaaca cgttggcgat ggcgatgtac cacagcagtt tgttcggcag
     7621 cacgtactgc atgaagattt gcttcgcggt cagctcctgt tccgcttttt cgttatagtc
     7681 gtccggataa tcatttttgt actcttcgat cggcggcaag ccacaggatt gcggggtatc
     7741 gcgcatcatc gcaaaggcga ataatgccac cagaatggcg cagaaagcag gcatatagag
     7801 cgccgcatgc cagtcattga accaggccat ccccagcagg aacagcagcg gcggaatacc
     7861 accaccgacg ttgtgcgcac agttccacac tgacacaatg ccgccacgtt ctttctgcga
     7921 ccaccagtgc accatagtac gaccacacgg cggccacccc atcccctgga accaaccgca
     7981 gaggaacaac agtacaaaca tcaccgcaat gctcgacgtc gcccatggca caaagcccat
     8041 aaacaacatc actgccgccg ccagaatcaa acctgcgggc aggaaaacgc gcggattcga
     8101 gcgatccgat accgaaccca tgatgaattt cgaaaatcca taagcaatcg agatccccga
     8161 aagggcaaaa cctaaatcac cgcgtgagaa tccctgctca accagataag gcatagcaag
     8221 cgcaaagttc ttacgaacca aatagtaagc cgcatagcca aagaatatcc ccaggaaaat
     8281 ttgccagcgc aatcgacgat aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg
     8341 tggcgctggt ttaaaaatac tcaacattga tagcctccgt ggcccgtggt cttatttatg
     8401 attaacagcc tgattcagtg agagaacctg ccgtttcttg agttgccgcg atgttaagaa
     8461 aacattcata aattaaatgt gaattgccgc acacattatt aaataagatt tacaaaatgt
     8521 tcaaaatgac gcatgaaatc acgttttact ttcgaatcat gagcgaatat gcgcgaaatc
     8581 aaacaattca tgtttttact atggctaaat ggtaaaaaac gaacttcaga gggataacaa
     8641 tgaaaactcg cgactcgcaa tcaagtgacg tgattatcat tggcggcggc gcaacgggag
     8701 ccgggattgc ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg
     8761 acatcgcaac cggtgccacc gggcgtaacc acggcctgct gcacagcggt gcgcgctatg
     8821 cagtaaccga tgcggaatcg gcccgcgaat gcattagtga aaaccagatc ctgaaacgca
     8881 ttgcacgtca ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg gaagatgacc
     8941 tctcattcca ggccactttt attcgcgcct gcgaagaagc agggatcagc gcagaagcta
     9001 tagacccgca gcaagcgcgc attatcgaac ctgccgttaa cccggcactg attggcgcgg
     9061 tgaaagttcc ggatggcacc gttgatccat ttcgtctgac cgcagcaaac atgctggatg
     9121 ccaaagaaca cggtgccgtt atccttaccg ctcatgaagt cacggggctg attcgtgaag
     9181 gcgcgacggt gtgcggtgtt cgtgtacgta accatctcac cggcgaaact caggcccttc
     9241 atgcacctgt cgtggttaat gccgctggga tctgggggca acacattgcc gaatatgccg
     9301 atctgcgcat tcgcatgttc ccggcgaaag gatcgctgct gatcatggat caccgcatta
     9361 accagcatgt gatcaaccgc tgccgtaaac cttccgacgc cgatattctg gtgcctggcg
     9421 ataccatttc gctgattggt accacctctt tacgtattga ttacaacgag attgacgata
     9481 atcgagtgac ggcagaagag gttgatattc tgctgcgtga aggggaaaaa ctggcccccg
     9541 tgatggcgaa aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg gttgccagcg
     9601 atgacgaccc gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac catgctgaac
     9661 gcgatggtct ggacggattt atcaccatca ccggtggcaa actgatgacc tatcggctga
     9721 tggctgaatg ggctaccgac gcggtatgcc gcaaactggg caacacgcgc ccctgtacga
     9781 ctgccgatct ggcactgcct ggttcacaag aacccgctga agttaccttg cgtaaagtca
     9841 tctccctgcc tgccccgctg cgcggttctg cggtttatcg tcatggcgat cgcacgcctg
     9901 cctggctgag cgaaggccgt ctacaccgta gcctggtatg tgagtgcgaa gcggtaactg
     9961 cgggtgaagt gcagtacgcg gtagaaaatt taaacgttaa tagcctgctg gatttacgcc
    10021 gtcgtacccg tgtggggatg ggcacctgcc agggcgaact ctgcgcctgc cgcgctgccg
    10081 gactgctgca acggtttaac gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct
    10141 tccttaacga acgctggaaa ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa
    10201 gcgaatttac ccgctgggtt tatcagggac tgtgtggtct ggagaaggag cagaaagatg
    10261 cgctttgata ctgtcattat gggcggcggc ctcgccggat tactctgtgg cctgcaactg
    10321 caaaaacacg gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgtt gcatttctca
    10381 tccggttcgc tggatttgct gagccatctg ccagatggtc aacccgtggc agacattcac
    10441 agtggactgg aatctttgcg tcagcaggca cctgcacatc cttactctct tctcgggcca
    10501 caacgcgtgc tcgatctcgc ttgccaggcg caggcattaa tcgctgaaag cggtgcgcaa
    10561 ttgcagggca gcgtagaact ggctcaccag cggattacgc cgctcggcac tttgcgtgca
    10621 acctggttaa gttcgccaga agttcccgtc tggccgctac ccgcgaagaa gatatgtgta
    10681 gtggggataa gcgggctgat ggatttccag gcgcatcttg cggcggcttc gttgcgcgaa
    10741 ctggatcttt cggttgaaac cgcagaaata gagctgccgg aactggatgt cctgcgcaat
    10801 aacgccaccg aatttcgcgc ggtgaatatc gcccgtttcc ttgataatga agaaaactgg
    10861 ccgctgttac ttgatgcgct tattcctgtc gccaatacct gcgaaatgat cctgatgccc
    10921 gcctgcttcg gcctggccga tgacaaactg tggcgttggt tgaatgaaaa actaccttgt
    10981 tcactgatgc ttttgccaac gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag
    11041 ttacagcgcc agtttgtgcg ccaaggtgga gtgtggatgc cgggcgatga agtgaaaaaa
    11101 gtgacctgta aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc cgatattccg
    11161 ctacgtccac gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg actagtagcg
    11221 gaacgtaacg gcattcgcga gccgattctc ggccttgatg tgctacaaac cgccacgcgg
    11281 ggtgaatggt ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc
    11341 actgatgaga cgctacgccc gtcgcaggca gggcaaacca ttgagaacct gtttgccatc
    11401 ggttcggtgc tgggcggatt tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc
    11461 gtcagtgctt tacatgccgc tcaacagatt gcccaacgcg caggaggcca acaatgaacg
    11521 acaccagctt cgaaaactgc attaagtgca ccgtctgcac caccgcctgc ccggtgagcc
    11581 gggtgaatcc cggttatcca gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt
    11641 tgaaagatgg cgcactgtat gacgaggcgc tgaaatattg catcaactgc aaacgttgtg
    11701 aagtcgcctg cccgtccgat gtgaagattg gcgatattat ccagcgcgcg cgggcgaaat
    11761 atgacaccac gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta
    11821 gcgtttccac gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc
    11881 agctgcttga tgcggcgtta aaaatcgatc atcgtcgcac gctaccgaaa tactccttcg
    11941 gcacgttccg tcgctggtat cgcagcatag cggctcagca agcacaatat aaagaccagg
    12001 tcgctttctt tcacggctgc ttcgttaact acaaccatcc gcagttaggt aaagatttaa
    12061 ttaaagtgct caacgcaatg ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg
    12121 gcgtaccgct aatcgccaac ggctttaccg ataaagcacg caaacaggca attacgaatg
    12181 tagagtcgat ccgcgaagct gtgggagtaa aaggcattcc ggtgattgcc acctcctcaa
    12241 cctgtacatt tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac aacaaaggct
    12301 tgcgcgatca tatcgaactg gcaacccgct ggctgtggcg caagctggac gaaggcaaaa
    12361 cgttaccgct gaaaccgctg ccgctgaaag tggtttatca cactccgtgc catatggaaa
    12421 aaatgggctg gacgctctac accctggagc tgttgcgtaa catcccgggg cttgagttaa
    12481 cggtgctgga ttcccagtgc tgcggtattg cgggtactta cggtttcaaa aaagagaact
    12541 accccacctc acaagccatc ggcgcaccac tgttccgcca gatagaagaa agcggcgcag
    12601 atctggtggt caccgactgc gaaacctgta aatggcagat tgagatgtcc acaagtcttc
    12661 gctgcgaaca tccgattacg ctactggccc aggcgctggc ttaaactccc ttctgatgcc
    12721 cggtaagcat gtggttaccg ggcatttttg cgtacacgat tccgtgccca atgtatgcgt
    12781 tgcaacgcag tgaaaattcc tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca
    12841 aaggcctggg ataaccgtaa tgtcgcgtca tcataaatat caggtgacgg acaaccatga
    12901 ccgaatcaac aacctcctcc ccgcatgatg cggtatttaa aacctttata ttcacacccg
    12961 aaaccgcacg ggattttctc gaaatacatt taccagaacc actgcgcaag ctttgcaacc
    13021 tgcaaacctt acgcctggaa cccactagtt ttattgaaaa aagtttacgc gcttactact
    13081 cggatgtttt gtggtccgtg gaaaccagcg acggtgacgg ctatatctac tgcgtgattg
    13141 aacatcaaag ctctgcagaa aagaatatgg cttttcggcc aatgcgctat gccactgccg
    13201 ccatgcagag tcacctagat aaaggctatg acagagttcc gctggtggtg ccattgctgt
    13261 tttatcatgg cgaaacatcg ccctacccgt actcacttaa ctggctggat gagtttgacg
    13321 atccgcaact tgcccggcag ttgtacaccg aagcttttcc gttggtggat atcaccatcg
    13381 tacctgacga tgagatcatg caacatcggc gtatagctct gctggaactg attcaaaagc
    13441 atattcgcga ccacgattta atcggcatgg tcgacaggat caccacgctt ttggttagag
    13501 gcttcactaa tgacagccag ctacaaacac tgtttaatta tctgctgcaa tgcggcgata
    13561 cctcccgttt cacccgtttt attcaggaga ttgccgaacg ttcaccacta caaaaggaga
    13621 gattaatgac tattgctgaa cggctacggc aggaaggaca tcaaattggc tggcaagaag
    13681 gtaaattagt aggtttgcag caaggtaaat tagaaggttt gcaggaaggt atgcatgaac
    13741 aagccattaa aattgctttg cgcatgctgg aacagggcat tgatcgtgac caagtgctcg
    13801 cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta acacaggccc
    13861 acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg gcccattttt
    13921 gcctgattta aacatcgcca gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac
    13981 gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat caggagccac
    14041 agccagaaaa ccagccgctt tacccgcagc acggatccgc cgaatgctgg tttcaataat
    14101 tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag aaagatccgc
    14161 tggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca ggttatccag
    14221 tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt gcgccatgta
    14281 attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac cacgctcacc
    14341 gtagggaggg ttggacaaat tttgccggag ggggaatgtg ccggtgcagt aaaccggcac
    14401 agagtggcga aatcagacgg ataaagtcat ggactcaaca acctcaatcc agccatgctc
    14461 actagcgatg tcctggccgt ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca
    14521 cacttcctga cggatagcca ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa
    14581 agtgccgtct ggcgtggcta gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc
    14641 cagtgccagc ccggcaaaat ggttggcccg ccgttctgta atccagtgcg cagtttgcgc
    14701 cagggtttct tcctgtgaag gaatcacttc acacgccagc aatggagcac ctgcgcgaga
    14761 aagttgcaaa gccaataaac caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg
    14821 gttttgcaat tcgcgactga tctgcgcggg cagtccttcg gtgccttcga aaatcacgct
    14881 ctgtccggca acacgtttaa cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc
    14941 cggtccggtg agtttcagtt cgatgatcgg cattgaggag cgatagccca ttgttacgcc
    15001 cggcggcagt tgtagagtgt ccaggctttg tgccagatcg ctttccgaac gaccaaaggt
    15061 agttaaacgc agacaaaccg gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag
    15121 gatttcgtgc tcgaccatca ccttaaattc tgacggtacg ccgggagtaa agaacatcag
    15181 gcaacgatta agctgcacgg caaaaccaca ggcggtgcct accgggttat tgataaattc
    15241 agcactggca ggcagctccg cttgtttacg gttgctcggt gccattactc gtccacgttc
    15301 gtggaaatag cgttccatct ctttgagcca ggcttcatgc agcaccaggc cttcaccttt
    15361 tgctgtcgca gcggcgagtg cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac
    15421 gatcagcaca tcggcgtgct gactacgttc gcgaagaatg gtgactaagt catcaaggtt
    15481 atcccccacc gtattgcggc gagataatgg cagcccctga tgaaagaaaa aatcggccag
    15541 ccaggcagcg ttagtgtcaa cgatttgccc gtataacact tcatccccgg tggatagcat
    15601 ttccactttt aacatggtta tctcctgctt ttgttatcga atcactataa cgcaaaggga
    15661 gataaggcgt agtaagaaac tgaatgccgc gccggaaggg atcagaaact ggcgttaacc
    15721 ccaacataca ggccgtcagc cacagcgttg tcgcggttac cgtctttacc cgacagattc
    15781 aggtagcgat aacccgcctc aatactgact ggacgcataa tggtgtaacg cgcgccagca
    15841 ttcgcttcct cataactttg aataccgctg gagagcgaat ccggagagta gtaatactcg
    15901 ccaaacaaac ggaagctgtt gccaattttc cactgcaaac cacctcctac cgctgcggca
    15961 taaccttcat cgccgtaatt cgggttggtg tacacgcctt ttccgccaac ggtcgccatt
    16021 aacggcccga gaggcagatt caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg
    16081 ttatgtgtcc agttaccgct taaagccagg cccgtcgatt cagtcccaaa accgacacca
    16141 atattggtgt attctttacc cgcctggccg ctgatgctca ttgcattgac cgatgcagaa
    16201 accaacagca ttccggccag acctgctagc gcaatttttt tcattatccc ctttcccaca
    16261 agcaattaat caaaattccc aaagctgcgg gattgtactg cctggcacag aggattcaat
    16321 gagcgaaaaa agggatctag tcacttattg taactaaata cttcttaaat aattaaacgc
    16381 cgaaaaaata aacacacttt accgatacac acaaaaataa taataagtgc agttatcatt
    16441 gcaaaagaga ttactgtcac tttcacatat ttctgagtta ccgtattctc atggtttctt
    16501 tttatttata aggaataccc ctgtgcgaca acggactatt gtatgccctt tgattcaaaa
    16561 tgatggtgct tatttgctgt gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg
    16621 ggcgctttcg ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga
    16681 aattcgcgaa gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag
    16741 cgatgatatt cgcaccaaga catatgcaga tggtcgcaag gaagagattt atatgattta
    16801 cctgattttt gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga
    16861 ctacgcgtgg gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa
    16921 aacgttacgt ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt
    16981 gaaaatttag tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat
    17041 gactaaacca tccagataat caggtttaaa tatcgcatca cgcttatttt tagcaatata
    17101 tgtcaggcaa tgattatggg tgaaaataac gatattttta tcaggcgctt tgttttgtaa
    17161 gtccttaatt gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag
    17221 ctttttaccc gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata
    17281 aagatcgaaa tcagagatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt
    17341 acctttaacc gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg
    17401 ttcagcatgg cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt
    17461 attgtcgata cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat
    17521 tgcagcgagc gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa
    17581 agctaacacg taaactccac ctatagacaa gcgcaaccag acaattaccg tgaaattgag
    17641 cgacatttct ggcgataatt agcagttggt gtaatattaa aaatcctacg atgtcggcaa
    17701 aatgcctcaa atttttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat
    17761 atttacgtct aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct
    17821 ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat
    17881 ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga
    17941 ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa
    18001 tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag
    18061 ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga
    18121 agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct tgctgggtgc
    18181 aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga
    18241 atcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagc
    18301 agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc
    18361 tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat
    18421 tttttcattt catgccatta aaaatattac ctgtgctgaa ggtggcctga ttgtaactga
    18481 taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc
    18541 ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa
    18601 gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagtca aattagagca
    18661 cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc
    18721 ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat
    18781 tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga
    18841 aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga
    18901 gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcgtt
    18961 gccgctgttc ccggatatga ctaccgccga tgccgaccgc gtcatcacag cccttcagca
    19021 actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat
    19081 tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg
    19141 tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc
    19201 cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgat
    19261 taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg ttactggcga
    19321 cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt
    19381 ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag
    19441 ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca ccactggcaa
    19501 agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat
    19561 gttgcactgc catgaacgca gcacctttat cccgattctg gcgaatatct tcgcccgccg
    19621 tgcaattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca aatacagttt
    19681 tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg
    19741 tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct
    19801 ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt
    19861 tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac tcggtgaata
    19921 tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat
    19981 ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg
    20041 ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc
    20101 cataccgata atcccggtga aaaagccttt tatggttcgg tggctcatct ggcggcggaa
    20161 agagacattc cggtttatgc gccggataac gttaatcatc cgctgtgggt ggaacgcatt
    20221 gcccaactgt caccagaggt gattttctct ttttattatc gccatcttat ttgcgacgag
    20281 atttttcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt accaaaatat
    20341 cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca
    20401 ttgcaccgaa tggtaaacgt gccgatgctg gggccattgt ggcgcaactg cgcgttgcca
    20461 ttgcgccaga cgatatcgct attacgctgc atcataaatt gtgccatgcc gcgcgccagc
    20521 tactggaaca gacattaccc gccattaaac acggtaatat tctggaaatc gcccagcgcg
    20581 aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga cagtttcctt gaatggcata
    20641 aaccggcatc cgtactgcac aacatggtac gtgccgttgc cgatccgtgg ccgggtgcct
    20701 tcagctatgt tggcaatcag aaattcaccg tctggtcgtc gcgtgttcat cctcatgcca
    20761 gcaaagcaca gccggggagc gtgatttctg ttgcgccact gctgattgcc tgtggcgatg
    20821 gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat tactatgcag ggctcgcaat
    20881 tagcgcagat gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct gcctgcaccg
    20941 cccgacgccg tacccgggta ctcatcctcg gggtgaatgg ctttattggc aaccatctga
    21001 cagaacgcct gctgcgcgaa gatcattatg aagtttacgg tctggatatt ggcagcgatg
    21061 cgataagccg ttttctgaat catccacatt ttcactttgt cgaaggcgat atcagtattc
    21121 attccgaatg gattgagtat cacgtcaaaa aatgtgatgt cgtcttgccg ctggtggcga
    21181 tagccacgcc gattgaatat acccgcaacc cgctgcgcgt atttgaactc gattttgaag
    21241 agaatctgcg cattatccgc tactgcgtta agtaccgtaa gcgaatcatc ttcccgtcaa
    21301 cttcagaagt ttatgggatg tgtagcgata aatacttcga tgaggaccat tctaatttaa
    21361 tcgtcggccc ggtgaataaa ccacgctgga tttattcggt gtcaaaacaa ttacttgatc
    21421 gggtgatctg ggcctatggc gaaaaagagg gtttacagtt caccctcttc ctcccgttta
    21481 actggatggg accacgactg gataacctta atgcagcgcg aattggcagc tcccgcgcta
    21541 ttacgcaact cattctcaat ctggtagaag gttcaccaat taagctaatt gatggcggaa
    21601 aacaaaaacg ctgctttact gatatccggg acggtatcga ggcgttgtac cacattatcg
    21661 aaaatgcggg aaatcgctgc gacggcgaaa ttatcaacat tggcaatcct gagaacgaag
    21721 cgagcattga ggaactgggc gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc
    21781 atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag tagctgctac tacggcaaag
    21841 gatatcagga cgtagagcat cgtaaaccga gcatccgcaa tgcccaccgc tgcctggact
    21901 gggagccgaa aattgatatg caggaaacca tcgacgaaac gctggatttc ttcctgcgca
    21961 ccgttgatct tacggataaa ccatcatgac caaagtaggc ttacgcattg atgtcgatgc
    22021 ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa atcttgagta agcataatat
    22081 tcaggccagc atttttttca gcgtcggccc ggacaatatg ggccgccatc tctggcgact
    22141 ggtgaagcca cagtttttgt ggaagatgct gcgctcaaac gcggcatcgc tttatggctg
    22201 ggatatttta ctggcaggta cggcctggcc aggtaaagag attggtcatg ccaatgccga
    22261 tatcattcgt gaagcggcta aacatcacga agtcggtctg cacgcctggg atcaccatgc
    22321 ctggcaagcc cgtagcggta actgggatcg gcaaacaatg atcgacgata ttgcacgcgg
    22381 tcttcgcact ctggaagaga ttatcggtca accggtaacc tgttctgccg ctgcgggctg
    22441 gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc catttgcgct acaacagcga
    22501 ttgtcgtggg gccataccgt tccgtccact gcttgaatca ggaaaccctg gcactgcgca
    22561 aattccggtg accttaccca cctgggatga agtgattggt cgggatgtga aagcagaaga
    22621 ttttaacggt tggttactca accgcatcct gcgagataaa ggcacgccgg tttataccat
    22681 tcatgcagaa gttgaaggct gcgcttatca gcataatttt gtggatctcc tcaaacgcgc
    22741 agctcaggaa ggcgtgacat tttgcccttt aagcgaactg ttatcagaga cgttgccgct
    22801 cggacaagtt gttcgcggaa atattgccgg acgtgaaggc tggctgggtt gccaacaaat
    22861 tgcgggtagt cgctgatgaa atcggtacgt taccttatcg gcatcttcgc gtttattgcc
    22921 tgctattacc tgttaccgat cagcacgcgt ctgctctggc aacccgatga aacgcgttat
    22981 gcggaaatca gtcgagaaat gctggcatcc ggcgactgga ttgtgcccca tctgttaggg
    23041 ctacgttatt tcgaaaaacc cattgccgga tactggatta acagcattgg gcaatggcta
    23101 tttggcgcga ataactttgg tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc
    23161 gcgctggtga cctggtttac tctgcgctta tggcgcgata aacgtctggc tctactcgcc
    23221 acagtaattt atctctcatt gtttattgtc tatgccatcg gcacttatgc cgtgctcgat
    23281 ccgtttattg cattctggct ggtggcggga atgtgcagct tctggctggc aatgcaggca
    23341 cagacgtgga aaggcaaaag cgcaggattt ttactgctgg gaatcacctg cggcatgggg
    23401 gtgatgacca aaggttttct cgcccttgcc gtgccggtat taagcgtgct gccatgggta
    23461 gcgacgcaaa aacgctggaa agatctcttt atttacggct ggctggcggt tatcagttgc
    23521 gtactgacgg ttctcccctg gggactggcg atagcgcagc gggagcctga cttctggcac
    23581 tattttttct gggttgagca tattcaacgc tttgcactag atgatgccca acatagagct
    23641 ccgttctggt actacgtgcc ggtcatcatt gccggtagcc tgccgtggct gggattactc
    23701 cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt ccgcaaccgt ctatttgttg
    23761 agctggacga taatgccgct gctgtttttc tccgtcgcta aaggcaaatt gcccacctat
    23821 attctttctt gctttgcacc tctggcaatg ctgttggcgc attacgcgtt gctggcagca
    23881 aaaaataatc ctctggcgct gcggattaat ggctggatta acatcgcttt tggcgtcact
    23941 ggcattattg ccacatttgt gatctccccc tggggaccaa tgaacacgcc ggtgtggcaa
    24001 accttcgaga gctataaagt cttttgtgcc tggtcgattt tttcgctatg ggcatttttc
    24061 ggctggtaca ccttaacaaa cgtcgaaaag acctggtctt tcgccgcgct ttgcccgctg
    24121 gggctggcgt tgctggtagg attttcaatt cctgacagag tcatggaagg aaaacatccg
    24181 caattttttg tcgagatgac acaagaatca cttcagccaa gccgctatat tcttactgat
    24241 agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac gcgatgacat catcatgtat
    24301 cgccagacag gtgagttgaa atacggcctt aattatccgg atgcgaaagg gagatttgtc
    24361 agcggtgatg agttcgcaaa ctggcttaat caacatcgtc aggaggggat tattacactc
    24421 gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg ccattccgcc cgcagatgcc
    24481 atcgttcgtc aggagcgtct ggtgctgatt cagtatcgtc ccaaatgatc tggctaacat
    24541 tagtctttgc cagcttgctt agcgttgccg ggcagttgtg tcagaaacag gcaacctgct
    24601 ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg gcgctggctt
    24661 gtcttggtct tgccatgatg ctctggctgc tggtcttgca gaacgtaccg gtaggcattg
    24721 cctacccgat gttaagtctg aattttgtct gggtgacgct ggctgcagta aaactgtggc
    24781 acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt cattattggt ggcattgtga
    24841 tcctcgggag tacggtgtaa tgtgcctgat atggggatta ttcagcgtca ttattgcctc
    24901 agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg ccgccgatga cgcacttatg
    24961 ggattttatc gcggctctgt tagcttttgg cctggatgcc cggatactgt tactcgggct
    25021 gctgggttat ctgctttccg ttttttgttg gtataaaacg ctacataaac ttgcgctaag
    25081 caaagcctat gccttgctca gtatgagtta tgtgctggta tggattgctt caatggtact
    25141 tcccggctgg gaagggactt tttcgctgaa agcactactg ggagtagcct gtattatgag
    25201 cgggttgatg ctgattttcc tgcccacgac aaaacaacgt tactgagttt tccctgccgc
    25261 tgtacactgc cgttcccact catcggggct ataactgctt gccgagagga ctttcaccgt
    25321 atgcagcttt tcacgattaa tacaataaag cgcattctgc aaaggcgaga gtaaatcccc
    25381 cactttcacg gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc cgccagcatc
    25441 gcagagcata agtacatgtc tgttatcttg tttattgcag cacgattttt tgtgccagcc
    25501 attccattgc attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata
    25561 aactctttat caacggcaat ggaatggcag aaatgagaag tgaaacggag ggttaattat
    25621 tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc
    25681 agctccggcg gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag cttatctttc
    25741 acccattcgc caagatcaac ggtttgctgg tcatactcca ccaccgctac cggccgatga
    25801 ccaaactcct tgtcggcaac ggaaacgata aacacctgca aaaccgcagg atgtgcagca
    25861 ataacacgct cgacttcttc cggctgaata ccctcaccgc cactgaagaa cagattgtct
    25921 aaacgtccga caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac
    25981 cagccttcgt cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata
    26041 ctggcagccc gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc
    26101 aaaccaacgt ctgccaggcc gtcggcttct ttcgcacata ccgtggaggc aaactcggtc
    26161 agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc
    26221 gggatagccg cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc
    26281 aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc
    26341 aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt
    26401 ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac
    26461 ggaatcagcg ataacacacc ttgcgcactg gcaagatggg cctgataagt atggacagcg
    26521 gctttcggca aaccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc
    26581 cacgcagcgg catgtacgcc ttcaaccagc tgaatgtgca gcgacgttaa tgccggaaac
    26641 gtgttttccc catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca
    26701 agcaacggtt gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac
    26761 gccagccagg cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc
    26821 tcaaccaccc cctgcgccgc aaagcccgta gctaactcat cgacgcgagc acaaagctcg
    26881 cgccagttga gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc
    26941 cagtgacgcc acggccagtc agagaagatc ataacaactg ctcctgtaca tcagcgtcca
    27001 ctaacggcag tgggctcccc ggccagcgac gaacctgctg cgcctgcatc agatccagcg
    27061 tgtccagtcc cggaatggtg tctggcgtta accaggcagc aatccgcgcc agttgcgtta
    27121 gacctaagct cgactcaatg gaagaactga tcaccgccgt cagccccagc gcatgcgccg
    27181 cctgtacctg ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg ataactaccg
    27241 cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg
    27301 caatggcaat gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct
    27361 cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctgac
    27421 ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct
    27481 ccagcaacag attcaccacc atgccgtcgc gcaccgcttc gtacaatccc actttgacct
    27541 tcgccacttt ctcgcccggc atatcggcaa gtttgaggat cagatcgtcc ggatcgccat
    27601 tacacagcgg tgccgcacgg tagttggctg cttgcggcaa cgtatctgtc agttctgcca
    27661 atgcacagct tacgccaaag gccacggaag gcatctgcgg tagctcgcaa tcgcctgcca
    27721 gccagttatt tacccaggca agcagcacgc tttgcgcctc ttcccagttt tcctggctga
    27781 agcccggcag tggggagatc tccccccacc cttcgcgctc gccctcacgc aggcaaacgt
    27841 acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac cacccccgcg tccatgggga
    27901 tctgccagcg gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc
    27961 aggctgacgt ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa
    28021 cagcatggtg gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt
    28081 cagcgcagct ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca
    28141 acggacggtt tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc
    28201 cagcgcctgt tttgcgtcgt actgacggca caggaaccag atttctcgcg cttttttctg
    28261 cccgacgata cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg
    28321 gccagtctga ccgaagatgg cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag
    28381 aacgtgacca ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt
    28441 acggatctga cgctggaagt ccagcacgtt caggtgatgt acgccggaat catctttata
    28501 gccgccgtaa tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc
    28561 gccagtcaga atgatcacgc cgatattgtc gtcataacgc gcatctgcca gtgcctggat
    28621 catctctttt accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat
    28681 ttttgcgata ccgtcggtgg atttttcata acgaatgtcc tcgaaacctt cggagcagtc
    28741 gtgccattca accggtgcgt aaggcattgc ttcatcagga taaatcatat tgtgtccttt
    28801 agtcagaaac gcaagatctg cgccagactt gcgattacgc cagcgggatt ttcccgatgc
    28861 gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccagggcg
    28921 cggaatttgc tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg
    28981 ttagcacgta aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt
    29041 gcgccattat tgttgctgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag
    29101 gcaaaaacag gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa
    29161 cgctgcgccc attggcgatc ggaacgctga cgttccgcac gttgttcagc attttgcagc
    29221 cccggatgcc cgccttcgac aataacccca caaagccccg ccagcccctg gcaagccgcc
    29281 atcatcgcca cccgtccgcc aagcgagtac cccaccagcc agaagtcaag gatgttgtaa
    29341 ctaaccaagg ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc
    29401 gccgaaccac catgacctag gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg
    29461 cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt
    29521 aaacctagtt ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca
    29581 gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac caccatttca atcaccgtgg
    29641 tggttggcgt gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag ttttgcggat
    29701 gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca
    29761 gatagaaacg ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt
    29821 tgttgttcac cacaattaat accagcggcg cagaaacctg acgcaataac gccagcgcgt
    29881 tgagatcgta aagcgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc
    29941 gctgaacgcc tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct cggttgctgt
    30001 acaccgggta acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt
    30061 taccaacaaa caattgcccc tgttcaggca gatagtcgct gatgcgatgc gccagttgcg
    30121 cttcgccaaa cgcatcacgg cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg
    30181 ggatttcaac gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa
    30241 tattggcaat taagcgacgt ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat
    30301 caacaatcca gtactcttct ggttcacagc ttgcctgcca ttgcaggagc cgtttccccg
    30361 tcaggctact tcccagttgc accacaattt gcgcctgctg cagctcgctg gtcgctttgg
    30421 cattgcctaa ccaaagatcg gcacacggca gcggctgccc ggtttgtgac agcacatcgc
    30481 caatcagcgg ccagccaaga gtttgcgccc acagggcaac ttttttgccc tcttccgcac
    30541 tcatgcgccc ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt
    30601 tttcactttc cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcac
    30661 ccagacgctg ttgccagcta agcccggtat cgtccatttc gccatacagc ggttcagcaa
    30721 acgggcagtt gatatggact ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag
    30781 aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg
    30841 ggtgagaggc gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct
    30901 ccggcgggcg atcggcggtt aagagaatca gtttttctcc ggttaacccg gcttcaatca
    30961 gtgccggata gagatttgcc accgccgtgc cggaggtcac aatcaccgcc accggctgct
    31021 tgctgacttt cgccagcccc agcgccagat gccccaaccc acgctcatcg aaatgggtgt
    31081 ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagcctg
    31141 gggcgataca gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc
    31201 agcgtcggtt aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt
    31261 ataaggagct acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat
    31321 tccatttgta ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc
    31381 tcggggtcgg aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta
    31441 atttttgctg agcgcaggga aacgcagaat tcgctttgtt gtaatgagag atagcccgca
    31501 gaaccggcgt accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga
    31561 tcgcgcggta agccagcaac tgctgccgtt ggctgcaact gatgtaaaca gatcacatca
    31621 tccgctttgt tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc
    31681 agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg ttggcagata
    31741 tcttccacca ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag ccactctcct
    31801 aactgctgcg cctgcttatc atcaggatga tttgctactg ttcccgccag cgcttcagta
    31861 cgcagcgctt tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt
    31921 tcgccatcaa aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc
    31981 atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc
    32041 actttgtcga gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag
    32101 cccgttttgt ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc
    32161 ttgatactca ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg ctgaagggag
    32221 ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg
    32281 cggggtaaaa gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag
    32341 tcagcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg
    32401 gtaatcgcgc ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc
    32461 gggtacgttt gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa
    32521 ggaatatcga taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt
    32581 agattttcca gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac
    32641 attatacggg gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt
    32701 gccagcaaca gtcctagtac cagcccgacg gccgcgccca caccaattcc ttgccagggt
    32761 ttttcgtgga cgtagtcatc agcacgataa acagcctgct tcgcccgata gtaataactg
    32821 tctgaagcct ggctaacccg ttttttcaca tcatccagcg ctttttctgc atgcgctttc
    32881 agctcaacat atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt
    32941 tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc
    33001 gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa gagttgttga ttacgcctga
    33061 attacctcgc gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca
    33121 gggataaaac cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca
    33181 ggcttatcag gccagtgatg cgtacatgtt tcgagtgttt tactcatcag ctgctgcccc
    33241 actttttcac cacgcaacgc ttcactgaca atcacccgac ctataacgac cggctcaaga
    33301 tcatcatcac ttttcagaat cctcgcatac gccaccagtt catcattttt ccaaccgaga
    33361 atatggcgat tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc
    33421 tcgacaacaa acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat
    33481 tcagagtgat gcagatcttg ccattcaatc ataatgctct ccttttagat gtatgcctta
    33541 tactagccgg aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact
    33601 tcagccggtg tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat
    33661 ccgacccaga gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg
    33721 cataccgcct ttaaccctgg aaaactggac aagattttta tcagtcacct tcatggcgat
    33781 catctttttg gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc
    33841 ttaacgattt atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc
    33901 ggctcatgga ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat
    33961 gatggcctgc gcaaagtaac cgcttatccg ctggaacacc cactggaatg ttatggctat
    34021 cgtattgaag aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc
    34081 gtgccgcctg gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat
    34141 ggaaggcaga ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct
    34201 attttcggcg ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc
    34261 atggtgcacg aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat
    34321 agctctacac gccaggctgc gacactagcc cgtgaggctg gagtcggcaa gctaatcatt
    34381 acccacgtca gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg
    34441 tcaattttcc cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactctgc
    34501 ttattattaa aaacagggtg aaacttgccc tgttatagca acccgcgcta catatatcca
    34561 cccacacagg catattgagc gatttttgat atttattcat ctccttacgc actatattca
    34621 gagcaataag ttgcctcttt tcatcttgat tcagcacaac ctttgcaact aaaaggagcc
    34681 catgatggtt acagttgtca gcaattattg tcaattatct caaacccaac tcagtcagac
    34741 atttgcagaa aaatttactg tgaccgagga attactgcag tctttaaaaa aaacagcgtt
    34801 atccggagat gaagaaagta ttgagttact gcataatatt gcgttaggtt atgatgaatt
    34861 tggaaaaaaa gctgggtaat gctgccaact tactgattta gtg
//
LOCUS       DAZSNO010000027        34688 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZSNO010000027 DAZSNO010000000
VERSION     DAZSNO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34688
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.027"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..2098
                     /locus_tag="ACHWN2_001282"
     CDS             <1..2098
                     /locus_tag="ACHWN2_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR8930891.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="ACHWN2_001283"
     CDS             2095..3183
                     /locus_tag="ACHWN2_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR8930892.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHWN2_001284"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHWN2_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFR8930893.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHWN2_001285"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHWN2_001285"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR8930894.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHWN2_001286"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHWN2_001286"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR8930895.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="ACHWN2_001287"
     CDS             8051..8602
                     /locus_tag="ACHWN2_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR8930896.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHWN2_001288"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHWN2_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR8930897.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACHWN2_001289"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACHWN2_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR8930898.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="ACHWN2_001290"
     CDS             complement(11683..12681)
                     /locus_tag="ACHWN2_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR8930899.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="ACHWN2_001291"
     CDS             complement(12760..13452)
                     /locus_tag="ACHWN2_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR8930900.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="ACHWN2_001292"
     CDS             complement(13529..14032)
                     /locus_tag="ACHWN2_001292"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR8930901.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="ACHWN2_001293"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="ACHWN2_001293"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR8930902.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="ACHWN2_001294"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="ACHWN2_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR8930903.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="ACHWN2_001295"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="ACHWN2_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR8930904.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACHWN2_001296"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACHWN2_001296"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR8930905.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACHWN2_001297"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACHWN2_001297"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR8930906.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACHWN2_001298"
                     /pseudo
     CDS             complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACHWN2_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(22127..23560)
                     /locus_tag="ACHWN2_001299"
     CDS             complement(22127..23560)
                     /locus_tag="ACHWN2_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8930907.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="ACHWN2_001300"
     CDS             complement(23623..24072)
                     /locus_tag="ACHWN2_001300"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR8930908.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACHWN2_001301"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACHWN2_001301"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFR8930909.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACHWN2_001302"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACHWN2_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR8930910.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="ACHWN2_001303"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="ACHWN2_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACHWN2_001304"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACHWN2_001304"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR8930911.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="ACHWN2_001305"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="ACHWN2_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32280..32903)
                     /gene="nfeR"
                     /locus_tag="ACHWN2_001306"
                     /gene_synonym="yqjI"
     CDS             complement(32280..32903)
                     /gene="nfeR"
                     /locus_tag="ACHWN2_001306"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR8930912.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33180..33925
                     /gene="nfeF"
                     /locus_tag="ACHWN2_001307"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             33180..33925
                     /gene="nfeF"
                     /locus_tag="ACHWN2_001307"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
     gene            complement(33979..34054)
                     /locus_tag="ACHWN2_001308"
     tRNA            complement(33979..34054)
                     /locus_tag="ACHWN2_001308"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34019..34021),aa:Ile,seq:cat)
     gene            34179..34685
                     /gene="mug"
                     /locus_tag="ACHWN2_001309"
                     /pseudo
     CDS             34179..34685
                     /gene="mug"
                     /locus_tag="ACHWN2_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8469 a         8920 c         9102 g         8197 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggcttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggt agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcaa ttgcaccgcg ccactggcgg atgcggcgat
    32281 taatccagtt gcgtaatatc aaaagcggcg cggtcgatca ccgcaatgat cttttttatt
    32341 tgtgcatcac tgatatcgct ctggttgacg cgtaaatcca gcaccgcttt aaaattatcc
    32401 agcgcccgct tcatttgcgg gttctggcgc agcgccgcgc caacgcaacg cgctttgatg
    32461 cgttcttcaa tcatctccac ttgttcgcgg ttttcttcca gccactgcgc gccttgttcg
    32521 gtcagcgcaa tctgcttctt accaccttcc tcttcgcgga tggtaatcag cgactgctcc
    32581 tgcagaaaat ccagcgtcgg gtagatgacg cccgggcttg gggtgtaatt cccctgggtt
    32641 agattctcaa tcgctttaat caattcgtaa ccgtggctgt catcgcgcga gagaatatcc
    32701 agaatcacca gacgcaattc accgtgacca aagaaacgtt gccgacgacc gccgccgcga
    32761 ccgtggcgac caccgcaaca ttgaccgtgt tcatgctggt gaccgtgtcc gcagtgctcg
    32821 tgttctgact tctcaccttt gcagcagccc tcatggcgtg gctggccttc atgtttacaa
    32881 cacccttcgt gatgatggct catatcagcc tccttttgct tgattagata tatctaattt
    32941 atatctgatg tgagcattac tgcaagtaca aaaatgattt ttaaacgtgg aagcacctca
    33001 catctaggcc ggataagacg ttcacgccgc atccggcagc attgctatgg cactgaatgc
    33061 cttcacatac ttcagacata tcagttctac aaatcgcttg catttatcat gattaacaat
    33121 cattatcatt tgcgaatttt atttagatat atctgattac catcacgaag gcgataacaa
    33181 tgaataacac cccccgctac ccgcagcgcg ttcgcaatga tctgcgcttc cgtgaactga
    33241 ctgtgctacg cgttgagcgc atcagcgccg gttttcagcg cattgtcctt ggcggcgagg
    33301 cgttggacgg ttttacatcg cgcggctttg acgatcacag caaactcttc tttcctcaac
    33361 ctgacgctca ctttgtgccg ccaacggtaa cggaagagag catcgtctgg ccggaaggac
    33421 cacgcccacc gtcgcgtgac tatacgccac tgtatgacga actacgccat gaactggcga
    33481 tcgatttctt tattcacgac ggtggggtcg ccagcggctg ggcgataaac ttacggtggc
    33541 aggtccgcgc ggttcgctgg tggtgccgga agattacgcg tatcagctgt atgtctgcga
    33601 tgaatccgga atgcccgcac tgcgccgccg cctggaaacg ttgagcaaac ttgccgttaa
    33661 accgcaagtt agtgcgctgg ttagcgtgcg ggataacgcc tgtcaggatt atctcgcgca
    33721 ccttgatggt tttaatatcg aatggctggc acatgatgag caggcggtag atgcgcgtct
    33781 ggcgcagatg caaatccctg ccgatgatta cttcatctgg ataaccggcg aaggtaaagt
    33841 cgttaagaat ttaagccgcc gctttgaagc ggaacagtat gacccacagc gggtacgtgc
    33901 agcggcttac tggcacgcaa agtaactgat atataaaaag aaaaaggctg acgatttctc
    33961 gtcagccttt gctatatctg gtggcccctg ctggacttga accagcgacc aagcgattat
    34021 gagtcgcctg ctctaaccac tgagctaagg ggccatggta gcggattata aagtaactcc
    34081 gtgccgcaat ccagccatta ccgcgcgcct gctgttttta taaacaatgt attttcaatc
    34141 cgttatactt ttcctggtga tgttagaaag gagtaaatat ggttgaggat attttggctc
    34201 cagggttacg ggtcgtgttt tgcggtatca accctgggct ttcatccgcc gggactggtt
    34261 ttccctttgc tcatccggca aatcgcttct ggaaggtgat atatcaggcc gggtttaccg
    34321 accgtcagtt gaagccgcag gaggcacagc atctgctgga ttatcgttgt ggcatcacca
    34381 aactggtaga ccgtccaacg gtgcaagcca atgaagtttc aaagcaggag ctgcacgcag
    34441 gtgggcgtaa gctgattgaa aaaattgaag attatcagcc gcaggcgttg gctattctgg
    34501 gcaaacaagc atatgaacag ggattcagct agcgcggtgc acagtggggg aaacaaacgc
    34561 tcaccattgg ttcgacgcag atttgggtgc tgccaaatcc cagcggttta agtcgcgttt
    34621 cactggagaa actggttgaa gcgtatcgcg agctggacca ggcgctggta gtgcgtgggc
    34681 gataaaaa
//
LOCUS       DAZSNO010000028        33649 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZSNO010000028 DAZSNO010000000
VERSION     DAZSNO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33649
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.028"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            376..815
                     /gene="ycgY"
                     /locus_tag="ACHWN2_001310"
                     /pseudo
     CDS             376..815
                     /gene="ycgY"
                     /locus_tag="ACHWN2_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
     gene            complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHWN2_001311"
     CDS             complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHWN2_001311"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR8930913.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHWN2_001312"
     CDS             complement(2911..4329)
                     /gene="dhaM"
                     /locus_tag="ACHWN2_001312"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFR8930914.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHWN2_001313"
     CDS             complement(4340..4972)
                     /gene="dhaL"
                     /locus_tag="ACHWN2_001313"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFR8930915.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHWN2_001314"
     CDS             complement(4983..6053)
                     /gene="dhaK"
                     /locus_tag="ACHWN2_001314"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFR8930916.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHWN2_001315"
     CDS             6281..8200
                     /gene="dhaR"
                     /locus_tag="ACHWN2_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFR8930917.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(8300..11167)
                     /locus_tag="ACHWN2_001316"
     CDS             complement(8300..11167)
                     /locus_tag="ACHWN2_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR8930918.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(12012..13103)
                     /gene="ychF"
                     /locus_tag="ACHWN2_001317"
     CDS             complement(12012..13103)
                     /gene="ychF"
                     /locus_tag="ACHWN2_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR8930919.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(13220..13804)
                     /gene="pth"
                     /locus_tag="ACHWN2_001318"
     CDS             complement(13220..13804)
                     /gene="pth"
                     /locus_tag="ACHWN2_001318"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR8930920.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            14082..14360
                     /gene="ychH"
                     /locus_tag="ACHWN2_001319"
     CDS             14082..14360
                     /gene="ychH"
                     /locus_tag="ACHWN2_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR8930921.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(14415..16094)
                     /gene="dauA"
                     /locus_tag="ACHWN2_001320"
     CDS             complement(14415..16094)
                     /gene="dauA"
                     /locus_tag="ACHWN2_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR8930922.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(16219..17166)
                     /gene="prs"
                     /locus_tag="ACHWN2_001321"
     CDS             complement(16219..17166)
                     /gene="prs"
                     /locus_tag="ACHWN2_001321"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR8930923.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(17317..18168)
                     /gene="ispE"
                     /locus_tag="ACHWN2_001322"
     CDS             complement(17317..18168)
                     /gene="ispE"
                     /locus_tag="ACHWN2_001322"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR8930924.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(18168..18791)
                     /gene="lolB"
                     /locus_tag="ACHWN2_001323"
     CDS             complement(18168..18791)
                     /gene="lolB"
                     /locus_tag="ACHWN2_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR8930925.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            19005..20261
                     /gene="hemA"
                     /locus_tag="ACHWN2_001324"
     CDS             19005..20261
                     /gene="hemA"
                     /locus_tag="ACHWN2_001324"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR8930926.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            20303..21385
                     /gene="prfA"
                     /locus_tag="ACHWN2_001325"
     CDS             20303..21385
                     /gene="prfA"
                     /locus_tag="ACHWN2_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR8930927.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            21385..22218
                     /gene="prmC"
                     /locus_tag="ACHWN2_001326"
     CDS             21385..22218
                     /gene="prmC"
                     /locus_tag="ACHWN2_001326"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR8930928.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            22215..22607
                     /gene="sirB2"
                     /locus_tag="ACHWN2_001327"
     CDS             22215..22607
                     /gene="sirB2"
                     /locus_tag="ACHWN2_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR8930929.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            22611..23420
                     /gene="sirB1"
                     /locus_tag="ACHWN2_001328"
     CDS             22611..23420
                     /gene="sirB1"
                     /locus_tag="ACHWN2_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR8930930.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            23456..24310
                     /gene="kdsA"
                     /locus_tag="ACHWN2_001329"
     CDS             23456..24310
                     /gene="kdsA"
                     /locus_tag="ACHWN2_001329"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR8930931.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24459..24566)
                     /locus_tag="ACHWN2_001330"
     CDS             complement(24459..24566)
                     /locus_tag="ACHWN2_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR8930932.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(24971..26071)
                     /gene="chaA"
                     /locus_tag="ACHWN2_001331"
     CDS             complement(24971..26071)
                     /gene="chaA"
                     /locus_tag="ACHWN2_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR8930933.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            26341..26571
                     /gene="chaB"
                     /locus_tag="ACHWN2_001332"
     CDS             26341..26571
                     /gene="chaB"
                     /locus_tag="ACHWN2_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR8930934.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            26729..27424
                     /gene="chaC"
                     /locus_tag="ACHWN2_001333"
     CDS             26729..27424
                     /gene="chaC"
                     /locus_tag="ACHWN2_001333"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR8930935.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(27468..27821)
                     /gene="ychN"
                     /locus_tag="ACHWN2_001334"
     CDS             complement(27468..27821)
                     /gene="ychN"
                     /locus_tag="ACHWN2_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR8930936.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            28006..29400
                     /gene="ychO"
                     /locus_tag="ACHWN2_001335"
                     /gene_synonym="iatA"
     CDS             28006..29400
                     /gene="ychO"
                     /locus_tag="ACHWN2_001335"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR8930937.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(29401..30051)
                     /gene="narL"
                     /locus_tag="ACHWN2_001336"
     CDS             complement(29401..30051)
                     /gene="narL"
                     /locus_tag="ACHWN2_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR8930938.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(30044..31840)
                     /gene="narX"
                     /locus_tag="ACHWN2_001337"
     CDS             complement(30044..31840)
                     /gene="narX"
                     /locus_tag="ACHWN2_001337"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR8930939.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            32179..33570
                     /gene="narK"
                     /locus_tag="ACHWN2_001338"
     CDS             32179..33570
                     /gene="narK"
                     /locus_tag="ACHWN2_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR8930940.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         8262 a         8320 c         8575 g         8492 t
ORIGIN      
        1 cattttatta atgcatttct cattcgggtg aaatttccgc tacctggacg cataaaaccg
       61 gaactctcgc caaaagtcgg gcactccgaa ggaattgacg cgttgcgctg ctctgtaacg
      121 gcatttaccg caagccattt atttcgccag cgacaatccg cagagaacca aacgcatcag
      181 gaaacgactg aacacaataa atactgtatt gatcaacata ttatactggc acctccagca
      241 cgcagtgagg atacactgta tcatcagcga atcttatcac tgcggagctg gttgaacgac
      301 tttctgagcg aaataagtcg gttcccggta acaacaacag aatccttttt taattattgt
      361 ttcgttgttt ttggtgtgat tcagaaaata aatattttag acagtagcca gtcaggcgtt
      421 aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt agaattgatc
      481 aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag
      541 gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga ttatgtgatt
      601 attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa caatattcgg
      661 tagcaattga tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa
      721 cacttacccg tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat
      781 acttcccgcc aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc
      841 tgccgacaac gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt
      901 gtgggttgtg cctctttggt tgagggttgc gtcgttgctg acttaacggt cggatgcgtc
      961 gccggaacat tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc
     1021 acgccattgg tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt
     1081 ccggtggtgc tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga
     1141 acattggtca ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt
     1201 aatccttctg tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc
     1261 ccacttttca ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc
     1321 gtcgccattt tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg
     1381 gccgcggtta actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct
     1441 tgttgatcgt tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg
     1501 tactggtttg ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc
     1561 attttaaaca tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt
     1621 aactgctgcg ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag
     1681 cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata
     1741 tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg
     1801 gtactatcct gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca
     1861 ccgtccatcc agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca
     1921 tcgccttcat gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg
     1981 cttaaatagt aactgcggtt gccgttggga atatgaccgt aagtgtttat ttcatgagca
     2041 aaattggcca ccatatccgc gattttatcc cagtgaccac tttcggcaag tcctaacatg
     2101 gtgaagtaat tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt
     2161 tccggcagcg gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc
     2221 ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg
     2281 ccttctttcg gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg
     2341 ttttgctgca tccgataatc agcaaggatc atcagcggat cgctgttagg cacggcatcg
     2401 gcaaaggttt tttggtccgg aaaaagtttg acgttttgca catcattaaa cagcggccct
     2461 aataaaatat caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca
     2521 gcgaaacaca aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg
     2581 ggggatttca tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag
     2641 aaaaccttag ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaacggtca
     2701 ggcattttag aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac
     2761 gtctgtttat tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata
     2821 aattggtaaa aaatataaca ggtagttaaa aaacatcagt gctgggtaaa ttgccggatg
     2881 acatcagaac gatgccatcc gaacggtggc ttaaccctga cggttgaaac gttgcgtttt
     2941 aacgtccagc gttagcgttt cttctggttg tatcgcatac agtttctcac cctgctggca
     3001 aatccagcca atccccagtt cacgggcgat tagggcgctg tgggataccg gacttccggc
     3061 gctaaggcag atacctttta caaccgccgg atccagttgc agtactgtgg aaggataaat
     3121 gttctccgcc agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt
     3181 caggtggacc agggtgcgat gcagaagatc gtccacatca atatagcgag cttgtagata
     3241 ttcatcatcc agttgctggt attgctggct aagttcttta agaacttgct gccaggcata
     3301 ttctgccgtg caatgttcat gctgaaggag ttcgcttgcc gccgccagca gttccggatc
     3361 acctaacagt gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggcttc
     3421 tgctttcgct gttaacgtca tcagatctaa taacgtgaag tcaatagcct ggcgtaatcg
     3481 atcttgttct tcttccgcgg tcagggttga ttttgcctgt accgtacata aaactggttg
     3541 ataataaaag gctttacccg aaacaggcag aacgggacgc agagtaggtg gagcgacttc
     3601 ctccgtttca ccaaagttat cttcagccag gtgacggaaa gcgatcagtg cctcttcagc
     3661 ttctggccct ttcgcaatca ggcgcagcgt atcgttatag cgaacttgta gtaacgcaat
     3721 ctggttaata ctctctggtg tgacgcattt gccgtttttt tccagcaaca tatcggcatt
     3781 aaatgtcgat aaggtataaa ccagccggga ggccggacgt acatgcaggc cgttacggtt
     3841 ttttatgacc accgccagag aacgggcttc ttcatcgtac ggaggacatg tgtcagagat
     3901 ttcagtgtcg gaggacggta aacccagttg ttcacgtttg gcttccagcg catgcatagc
     3961 gtcaaagata actttgtcga tatccgcccc cgaggccgcg ctgaccgttg ctgccagtgt
     4021 accttcgacc aacggcgcag cacacaaacg tacttttgcg gcgatctcgg gagccagcaa
     4081 ttccagcgca gtttcagcac tcaataatgc gctacccata tccatcatga ccagcacatg
     4141 gtcggcatca gcaacagatt cgatggcctc catcactttg acggcatcgg taccaatggg
     4201 attttgtgga tcgtcaattc ccgcggcaat ggcgatttta caactatcac tcattaacat
     4261 ctgacgggct aattcaccga caccttctcc cagtcggctg ctatgtgaaa ctatgaccag
     4321 gtttaccatc accaattcct tactcttttg cggctaacgc caacatttgc atcataaaca
     4381 tcaccgaggt cgcgccggga tcctggtgac caatactgcg ttcaccgaga taactggcgc
     4441 ggcctttgcg ggcttgcatc gtaatcgtac tttgtgcagc ggattcggcg atgctactgg
     4501 cagcttcgag cgccaccgga acagagagat tttgctcgct ggactgacgt aacgattcca
     4561 ccaccggcac ccacacatca cacatggttt tatcgccagg ttcggctttc ccgcgactga
     4621 ttacgccgtc cgcgccatcg cggaacatct gataaagctc ttccagtgtc aggctttgcc
     4681 gtgcctgggt cgcctgtgcg gcgcggataa agaaggtacc gaacagcgga ccactggcac
     4741 caccgacgct ggaaagcagc gtcataccgg tatttttgag aatgaaaccg atatctttat
     4801 ctgcgatagc agggagtttt tccaccactt tgctaaagcc tcggttcata tttagcccgt
     4861 ggtcagcgtc gccaatctcg cgatccagcc cggtaagata ctcgctctcg gtgttgaaaa
     4921 tatcgccaca acgagtgagc cagttaacaa tttgagttct gctcagtgac attgctttct
     4981 ccttatttac cccagttaag ggccggggtg tggaccgggg cgtcccagag agccagcgtt
     5041 tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca tatccagtga ggtgcagtac
     5101 gcgccaatta aattacgttc gatagtcaat cccgcttgct ggcaacgtgt ggtcaggcgg
     5161 ttatagacgc cgtacagctc agaaagcgga gttgcgccaa gattgttaac cagtgcaatc
     5221 acccgatcgc cagactggag cggttgtttg gtttgtggtt cttcctgcca gctgccttgt
     5281 tgataatccc agaaacgcaa agtgcgatgg tatgagccat gttccagcag ggtgtcgaac
     5341 atttcatcga cggtttgatc aagggaagag aaagggcggc ggtcaatacc tggctcacca
     5401 tgaatgccta cgccaaactc catctcatta tccgcaaggg taaaagaagg tttgcccgcg
     5461 gcaggaacgg tacaggcacc gagagcgata cctattgagt ggccttgatt attcaactta
     5521 cgccccagtt ccgcacaggc gtccagtgag tcgccacgct ccgccgctgc gcctacgagt
     5581 ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg cggtataaag actgtctttt
     5641 accgcaacgt cgtcatcaat gaccacagtg gtcactttta cgccgctatc gtgcagtaac
     5701 tcggtcgctg tttcaaagtt aagaatatcg ccggtgtaat ttttgataat caacagtaca
     5761 ccttcgccgc catcaatttg catggagcat tcaaagattt tatcgggcgt cggtgaggtg
     5821 aaaatttcgc ccggacaggc ccccgaaagc atcccctgac cgatataacc acagtgcatc
     5881 ggctcgtgtc cgctgccgcc acccgacagc agggcgactt ttcctgcaac aggggcatca
     5941 gctcgggtga catacaccgg atcctgatgc agtgtcagcg atggatgcgc tttcgccagt
     6001 cctgccagtt gttcgtccag tacgtcttgc acatcattga tcaatttttt cattattttg
     6061 ctccagcaat tacggtaggg catggatgat gttcaacgac acggcgacca ctgactgccg
     6121 atgaatccat tgtgcatcag gcaagggaaa agaaaattag cccaaattat gtttcatagt
     6181 gaaacatatg ctttaatgaa tgttccatat tgaaactttt acgtgtatta atacttaaaa
     6241 ttgcgagccg gaacaccttt tgtcataagg gatgcgggat atgagtggcg cttttaacaa
     6301 cgatggtcgg ggcatatctc ccttaattgc aacctcctgg gagcgatgca ataagctgat
     6361 gaaacgggag acatggaacg taccacatca ggcccagggc gtgacatttg cttctattta
     6421 tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg ctggaagatg cctgggaata
     6481 tatggcaccg cgagagtgtg cgctgtttat cctcgatgaa accgcctgca ttctcagccg
     6541 taatggcgat ccgcaaacct tgcagcagct aagtgcactg ggattcaatg acggcacgta
     6601 ttgcgccgag ggaattattg gtacttgtgc gctatcgtta gcggctatct ctggtcaggc
     6661 cgtgaaaacg atggccgatc aacatttcaa acaggcactc tggaactggg ccttttgtgc
     6721 aacgccgttg tttgacagca agggccgatt gacgggaaca atagcgctgg cgtgtccggt
     6781 tgagcaaact accgcagctg atttgccgtt gacgttggca atcgcccgcg aggtcggtaa
     6841 tttactgctg acggacagtt tgctcgctga aactaaccgt catttaaatc aacttaatgc
     6901 cctgttagaa agtatggatg atggcgtgat tagctgggac gagcagggta atttgcaatt
     6961 tattaatgcc caggcggcgc gggtcttgcg ccttgacgcg acggcaagtc agggacgggc
     7021 aatcactgaa ctcttaacgt tacccgccgt attgcaacaa gcaataaaac tggcacatcc
     7081 gctcaaacac gtagaagcaa cctttgaaag ccagcaccag tttattgatg cggtgataac
     7141 ccttaaaccg ataatagaaa cgcagggaac cagctttatt ttgttgctcc atcctgtgga
     7201 acagatgcgg cagttaatga ccagtcaatt aggaaaagtc agccatacct tcgctcatat
     7261 gccacaggac gatccgcaaa cccgccgctt gattcatttt ggtcgccagg cggcgcgcag
     7321 tagctttcct gtcctgcttt gtggagaaga gggcgtgggc aaggcactgc taagtcaggc
     7381 aattcataat gaaagcgagc gtgctgcagg tccttatatc gccgtcaatt gtgagttata
     7441 tggtgatgct gcgctggcgg aagaatttat tggtggcgat cgcacggaca atgaaaatgg
     7501 ccgtctgagt cggctggaac tggcacacgg cggcacgctg tttcttgaaa agattgaata
     7561 tctggcggtg gagttacagt ctgctttgct gcaggttatc aagcaggggg ttatcacgcg
     7621 actggatgcg cggcgtttaa taccaattga tgtcaaagtg attgcaacaa cgaccgcgga
     7681 cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgctgcatgc
     7741 atttgaaatt accatcccgc ctctgcgtat gcggcgtggc agcattccgg cgctggtgaa
     7801 taacaaatta cgcagtcttg aaaaacgctt ctctacgcgg ctgaaaattg atgacgatgc
     7861 cctcgctcgc ctagtttctt gtgcatggcc aggcaacgat tttgaacttt acagcgtcat
     7921 cgagaatctt gctctgagta gtgataacgg gcgcattcgc gtcagtgatt tacctgaaca
     7981 tctgtttacc gagcaggcga cagatgatgt cagcgccacc cgcctttcca ccagtctgtc
     8041 atttgcggaa gttgaaaaag aggcaattat taacgcagcc caggtcacag gcggtcgcat
     8101 tcaggaaatg tcggctttac ttgggatcgg ccgcactacg ctgtggcgga aaatgaagca
     8161 acatggcatt gatgcagggc agtttaagcg ccgggtatga aagacagaaa cgatttctga
     8221 tacatcagag tgatctgtat ttcattccgg cgcacgctaa caattttcag catcgtttaa
     8281 gggcttgtct atcccgcact taaaagctga agcgatatcc tccgttgact tgcttctgat
     8341 caaagaggtt accctgcgtg taatccgctt cgagatagaa tgtgtgctgt ttgttatact
     8401 gtgcactgac tcccacgccg ttattccagc cattaccttt gaaactgtac ttctcccggg
     8461 agtcgttcaa cagatattcg gtatcccctg aaaactcgcg gatagcgcca gtcttcacat
     8521 agacattcag ctggctgttg cctgcggtga tgtcataccc cagtatcatg ctggcacgcc
     8581 ccagcagcga ttcgtagtga ttcagatgta tattgaggcc attactcgcc ttcatagcca
     8641 tctcattctg gtggctgtat gtaagctggg tttgcggctc tatatagaac ccataaccag
     8701 taggggacag gttgaacctc tgcccggctt ccagggagat gctcattcca ttcgcagtgc
     8761 cgttggcgtt aacgccgttg ttctgactgt ccagtacgtg gaaactattt ttctggcgcg
     8821 atgcttttat aacgagatcg ctgtaaaaac cgttttgtgc catgtaactg gcgtacattc
     8881 ccatgtagtc tgaacgtgcg gtaccgtcgc ctccgctata gtccggcgat gcatgtgttg
     8941 agtcaatata cagaccgaca tacaacggca ttacatcaga gagacgttta tccccaccaa
     9001 actggatacc gctgtaaccc atgtcaaagc cgctcagttt gccactggca aaggagtcca
     9061 ggcttccccc ataactgcgc aaccagatat taccgtcttt actctgattt cgcaagtcac
     9121 ccatccgttg catcagcgta cggttttcaa catagttcaa taagtaaccg acattgagat
     9181 aattgccgcc agcatctgcg gtcgtggtgg gtttaggcgt gggagcgggt tcaggcgtag
     9241 gatcggggtt gactatggga ggctgagcgg gagctggtgt gggttcagga ttaggagtcg
     9301 gttccggaac tgtcccggaa gcgtaaagct cccagttagt gccattttta cgcacatcgt
     9361 acagatatcc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
     9421 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     9481 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ctattattaa
     9541 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     9601 tctctacgtt taatgtggta aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     9661 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     9721 tattcattgc aacgtccagt ttcccgccat tgacattatc gctgagggag gaacctgtcc
     9781 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaga acgctaccgt
     9841 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     9901 ttgcgatggc catctggtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     9961 tctggctgtt ggtcatggca taaatccctc tggctcctgc cgcgccggtg atagccaaac
    10021 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
    10081 gatcaaccgt aatatcggta ttttgcatgt tgataacagc cgttgccgtc tgggccgagg
    10141 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
    10201 ttgtcgcgtc tgaactactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
    10261 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
    10321 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgagcattat
    10381 cgccattggt tttaatggta ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
    10441 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtcgcc gtaaaacgcg
    10501 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
    10561 tcttgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
    10621 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
    10681 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
    10741 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
    10801 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
    10861 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
    10921 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
    10981 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
    11041 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
    11101 gcgaacggat ttttaattca gcgagacgat cggctttggt attcccattg tgttgtttga
    11161 tgcccatacc actcctatat agtacccagg ctatgggtct atggattttg tcattttgtt
    11221 taagtcaatg aataatatcc tcttatcatc atgatatctg tatggtgcaa tcatgatgag
    11281 cttctctgag aataattctc tattaccggg caaaatcagg aacacaggaa ttaatatata
    11341 tttaaccccc ccccttacaa aggtgagttt atatcgagtt attccctaag ttgtcgttac
    11401 ttattccaat tcgttactat gaatatggca aaaatattac aacaaacagc ggttaaaaaa
    11461 tttggtgaag attttttgta ataatcctaa ttagttatgg gattttcgct tgatgtatct
    11521 gcataacaaa aacaatatgc gccatttttg cattactaat aagaaaaagc aaaaaacatc
    11581 gatattcatt tttgttcaat ggattttctg gcctatcgtt attattgtgc tactggttat
    11641 cattttcatc ttgccaggat cgcacgtaat cacgttattc ggggacttta tctgcgggat
    11701 attacccttc aggtaagtcc ttttctcgcc agcatattgg agcgagatca atattgatca
    11761 gctgtgaaga gggctgatgt gtagtggaac aaaaactgac gttatatcac tgctaatgag
    11821 atatcagtca tctgaacact tatgtcttta attattctcg tggttcactg taggcaataa
    11881 gcacaaaagt gtaggatgtt acaagaatga ttaggactcg gtgaaatgaa aaatccacgc
    11941 aattgcgtgg attttatata cttttgcgct cttcatgaga tttagcgaaa cctcatgaga
    12001 caataaatta attagacgtt gaaaaggaag ttcatcacat cgccatcttt cacgatgtaa
    12061 tctttacctt ctgcacgcat tttgcctgct tctttcgcgc cttgttcacc tttgtaagta
    12121 atgaaatctt caaacgagat ggtttgtgca cggataaagc ctttttcaaa atcagtatgg
    12181 attttgcccg ctgcctgcgg cgcggttgct ccaaccggaa tggtccatgc acgcacttct
    12241 ttcaccccag cggtgaagta agtttgcagg ttcagcagct tataaccggc acggatcaca
    12301 cggttcaggc ccggttcttc cagcccaagc tcctgcataa actcgtcacg ttcttcgtcg
    12361 tccagttcgg caatgtctgc ttcaacagca gcacaaaccg gaaccacaac agaaccttct
    12421 ttcgccgcga tttcacgcac ctggtcaaga tatgggttgt tttcaaaacc atcttcgttg
    12481 acgttggcta tgtacattgt tggtttcagc gtcaggaagc tcaggtaacg aatagccgct
    12541 ttctcttcag cgcttaaatc cagcgcgcgc agcatacctg cgttttccag ctggggcagg
    12601 catttttcca ggaccgccag ctcagctttc gcgtctttat cgccaccttt ggctttcttc
    12661 tgtacgcgat gaatcgcacg ttcgcaggtg tcgaggtctg ccagcgccag ttcggtgttg
    12721 ataacttcaa tatcgtcagc cgggttaact ttgccggaaa cgtgaatgat gttgtcattt
    12781 tcaaagcagc gaacaacgtg accgatcgct tcggtttcac ggatgttggt caggaactgg
    12841 ttacccagac cttcgccttt cgatgcgcct tttaccagac cggcgatatc gacaaattcc
    12901 atggtcgtgg gaagcgtacg ttgcggtttt acgatttcag ccagttgatc caggcgaggg
    12961 tcaggcattg gtacgacgcc tgtgttcggc tcaatggtgc agaatggaaa gttggccgct
    13021 tcaataccgg ctttggtcag cgcgttgaac agggtagatt tcccgacgtt gggcaaaccg
    13081 acgataccgc atttgaatcc catgatttaa atcaccttaa tatcttaata atcaacctgt
    13141 tattgctaac agattgcaga aatggaaata actttgccta ttatacacgg cactcggcaa
    13201 aaatgccgca gacaacgact tattgcgctt taaaggcgtg caatcggttc gttgctttgg
    13261 tcaagccatc tgtaaaccac atttcagtac aacgcgccgc ttcgtcgatg gcttcatcaa
    13321 ttaacttctg ttcactaaca ggcggtttgc ctaacacaaa accgacaact ttatttttat
    13381 cgcccggatg accgattccg atgcgtaaac ggtgaaagtt agggttatta cccaatttac
    13441 tgatgatgtc tttcagtcca ttgtgaccac catggccacc gcccaactta aatttggcga
    13501 cgccaggagg cagatccagt tcgtcgtggg ccaccagaat ttcgtccgga ttaatgcgga
    13561 aaaaactggc catcgccgca acggctttgc cgctgagatt cataaatgta gtcgggacta
    13621 acaggcggac atcttcgctt ccaagggtga ctcgcgaagt ataaccaaag aatttagcct
    13681 cttcgcgcag cggagcgcgc aaacgctctg ccagtaagtc aacgaaccag gcaccagcat
    13741 tatgtcgcgt tgcggcgtat tcagcaccgg ggttcgccag gccgacaatc aatttaatcg
    13801 tcacgttttt ttgtcctgag tgtgtacata actggcgcgt agtttactgg ttgcggcccc
    13861 gcttgacaaa aaactgcgta tcaaatgcag ataacgtaat aattgcctga gtggactatt
    13921 agaaagtcaa ggtgttcagg cgtttatttg taaagttttg ttgaaataag ggttgtaatt
    13981 gtgattacgc ccgcacataa cccacagggt gttgtctata ctttacacat aaggaagagg
    14041 ggtattccct gttacaaccc agaaagttcc ggaggtgaca tatgaaacgc aaaaacgctt
    14101 cgatactcgg taacgtgctc atggggttgg gtctggtggt aatggtggtc ggcgtggggt
    14161 attcaatcct caaccagtta ccacagttta atatgcccca gtatttcgca catggtgcag
    14221 tgctaagtat tttcgtcggt gccattctct ggctggcggg tgcccgtgtt ggcgggcatg
    14281 aacaggtgtg cgaccgttac tggtgggttc gtcactatga caaacgttgc cgccgtagcg
    14341 ataatcgccg tcatagctaa caaataatgc agtttgctga ccagtcagtt tgcgctgact
    14401 ggtcaatctc gtacttataa atccgccatc gccgcgcgac gattcgggaa gaacgcgagg
    14461 cgtcccggga tcggttgaat gcctgcgcga gccatagttc gcagtggctg gaattccacg
    14521 ttgcacacgc gcagttcaca tccttcgggc agacgcttca caaaacgctg gaacgcatca
    14581 agaccaccag catcaagtac cggaacggca tcccacttca gaatcacaat ccgtttgcct
    14641 tcaagacgtg actccaggtc cgtgaataag ccttcagcag cagcaaaaaa cagcgggcca
    14701 ataacgcgca gaaccaggac atcgtctgga acatctacga ctaccggtgc caggcgagtc
    14761 atacgtgcga tacgacgcat aaacagcagc gatgccagca cgatccccac gctgatggca
    14821 ataaccatat caaacagcac ggtcagtgac atacacagca gcatgacgat gatgtcatct
    14881 ttcggcgcat gacgcagcaa gtcgaccact ttgtgcgctt cactcatgtt ccacgccacc
    14941 atcaacagca gggctgccat ggcggaaagc ggcagccagg agagcagtgg tgccagtacc
    15001 agcagggcga gaataaccag aatagagtgg atcaccgccg agatagggga agttgccccg
    15061 gcacggacgt tagcggcaga acgcgcgatg gcagcagtag cggtaatacc accaaagaat
    15121 ggagcgatga tattccccag cccctgaccg accagttcac tattcgcctt gtgcttagtc
    15181 ccggtcatac catccagtac cacagcgcag agcagagatt cgatcgcccc gagcattgcc
    15241 attgagaatg ccgcaggcag cagggtgcga atagaatccc aggttagcgt gaattctgaa
    15301 ttaggcagat cccacggcag caccagttgc ggcagcagtt gcggaatacc gttaccctga
    15361 gaaccatcgg ccaggacgta gtggaattgc gaaccgatgg tagcaacatg tccgccgagc
    15421 aggttaacaa tccccatcac cgcgcaacca gccagcaagg ccggaaggtg accgggtaaa
    15481 cgaatgccca gacgcggcca gaaaaccaga atgccaagcg tcacaatgcc aatggcagca
    15541 tcacccacat taatggtcgg cagcgccata aataatgcgc cgactttttg tagataatgt
    15601 tccgggacat gggccatttg cagaccgaga aaatctttaa tctgcatggt accgatggtg
    15661 atcccgatac ccgaggtgaa acctaaggtg acggaaaccg gaatatactc aatcagacga
    15721 ccaaagcgtg ccagccccat cagaatcaaa aagattcccg acaacaaggt cgcaaccagc
    15781 agtcctgcca gtccaaactg ttgcgaaacg ggatagagaa ttaccacaaa tgccgcagtc
    15841 ggaccggaaa cgctaaagcg tgacccaccc gttagagcaa tgacaatccc cgcaacagct
    15901 gcggtatata aaccgtactg gggggccaca ccactaccaa tagccaacgc catcgccagc
    15961 gggatagcaa taatcccgac ggttatcccg gcaatcaggt cacgggtaaa ccgtgcggca
    16021 gtatattttt ctttccagca aacgtcgatc agagcgcgga aaggcatcac atgtgaggaa
    16081 aatattttgt tcacaataat gtttcatccg tgagcgcatc atctgtcaac taaatggcag
    16141 gtgaaggagg cataggtcat acaaatggat attacagaca aaaaaacccg ccgcagcggg
    16201 tctttgagcc gggttcgatt agtgttcgaa catggcagag atcgattctt cgttgctgat
    16261 acgacgaatc gcttcggcca gcatacctga cagggtcaga gtacgcacgt tcggcagtga
    16321 tttgatttca tcgctcagcg gaatggtatc gcagacaacg acttcatcaa ttacagagtt
    16381 acgcaggttg ttcgccgcgt tgccagagaa gatcgggtga gtcgcgtacg caaatacacg
    16441 tttagcacca cgttctttca gcgcttcagc agctttacac agcgtaccgc cagtgtcgat
    16501 catatcatcg accagtacgc agtcacgacc tgcaacgtca ccgatgatat gcatcacctg
    16561 ggaaacgttc gcacgcggac gacgtttgtc gatgattgcc atatcggtat cgttcagcag
    16621 cttagcgata gcgcgggcac gcacaacgcc gccgatgtcc ggagaaacca caattgggtt
    16681 atccagattc agctgcagca tgtcttccag caggatcggg ctaccaaata cgttatcaac
    16741 cggaacgtcg aagaaaccct gaatctgttc agcgtgcaga tccactgtca gcacacggtc
    16801 aacaccgacg ctggagagga agtctgcaac cactttcgca gtgattggta cacgagcgga
    16861 acggacgcga cggtcctggc gcgcatagcc aaagtagggg ataacagcgg tgatacgacc
    16921 tgcggaagca cgacgcaggg catcaaccat aacgactaat tccatcaggt tgtcgttagt
    16981 aggggcacaa gtggactgga tgatgaaaat atcaccaccg cgtacatttt cgttaatttg
    17041 tacgctgact tcgccgtcgc taaagcgacc tacagcggcg tcgccgagtg aagtgtacag
    17101 gcggttggca atacgttgtg ctagtaccgg ggtggcgtta ccagcaaaaa gcttcatatc
    17161 aggcacgaga agaacctcag gcatgcgtcc attggtggaa agaatctgcc gaaaactgtg
    17221 cgggccaggc atgatccttt ccaggcggtg tattaaagag cgcgatgcaa cgtctggaac
    17281 aaggtgacgt tgtcaccgaa actcagcttg cccggcttaa agcatggctc tgtgcaatgg
    17341 ggaaagatta acgcctttcg ccacaaagcc attgagccat tccggggctt gctctagcac
    17401 ctggcgggct tcagactctg tatcaaattc agcaaagaca caggcccctg tcccagtcag
    17461 gcgtgacggg gcgtattcta acagccagga aagcaccgca tcaacctcgc gaaaacgttt
    17521 tcttgcgata acctcgcaat cattgctgaa ttcacatttt agcaacgttt ctattgacct
    17581 ttttggcgta ttgcgcggga gttcaggatc tttaaaaatc accggagtcg gaatacttac
    17641 accagggtgc gccaccagat accacttctc tggcggatcc accggcgtta gtatttcacc
    17701 aacgccttcg gcaaacgcgg catgcccccg aacaaagaca ggaacatctg cgcccagcgt
    17761 cagccccatt tccgccagct catccatgct tagcccgcat tgccagagat gatttaatgc
    17821 caccaggacc gtcgcggcat tggatgaacc accgccgaga ccgccgccca tcggcaaacg
    17881 cttgtcaatg ctgatattcg caccgcttcc cgtcggaaga cgcccgctgt ctgccgcagt
    17941 tttcatcaac aaccgcgctg cgcgaacgat caggttatct tcatgttcca cgccttcaac
    18001 gggcgttaac agacgaatat ccccatcgtc acgaagctca atgctgatgg tgtcgccgta
    18061 atcaagaaac tgaaacagcg tttgcagcgt gtggtaacca tccgcacgct gaccggtaat
    18121 gtataaaaac agattaagtt ttgccggaga gggccactgt gtccgcatta tttcactatc
    18181 cagttatcca tttttaactt gatgcgttga ccaccgtcgg tgagttccat attggctggc
    18241 atcgcaggtt gcgttttggt gtcataacca ccataaacaa ccttccagtt tttgccattc
    18301 tggctgtagg taatttcgct caggcggtac tggtcgtcca gtttgtagtc ggttgcatca
    18361 cccggtaaac ctaaaatcca ctggcgcaag ctgttgagcg gaattggcat tccggtcaat
    18421 ttgccaatca tctcttcggc gtcatcggcg gtataacgct gacctttatt gtcgactaac
    18481 tgcacgttac ccggttgagc attcagctcc agttccgtgc tgcccaatgg gttagtgagc
    18541 agcagacggt agcgatcctg gccggtttgc tgccagaaaa agcgggcgta cactttttgt
    18601 tggtcagaaa tataagcgaa cgcgccgcga gtctgatact gattaagatt gcgcacgtct
    18661 tgctgatgct gacgccattg tggcgaatcc gggcttttgc caggaccttt gggcgtggta
    18721 acggaacagg cagtgagcac aagagcagcc agcggtagca ggcggataag acgaaaatcg
    18781 ggcaggggca tagtgatgac aagtccttga gatacgttgc agttataacc cttaatgcta
    18841 gcgttaccgt ccgctatcgt ctatgttcaa gttgtcttaa ttgccagaat ctaacggctt
    18901 tcggcaatta ctccaaaagg gggcgctctc ttttattgat cttacgcatc ctgtatgatg
    18961 caagcagact aaccctatca acgttggtat tatttcccgc agacatgacc cttttagcac
    19021 tcggtatcaa ccataaaacg gcacctgtat cgctgcgaga acgtgtatcg ttttcgccgg
    19081 ataagctcga tcaggcgctt gacagcctgc ttgcgcagcc gatggtgcag ggcggcgtgg
    19141 tgctgtcgac gtgcaaccgc acggaacttt atcttagcgt tgaagagcag gataacctgc
    19201 aagaggcgtt aatccgctgg ctttgcgatt atcacaatct taatgaagaa gatctgcgta
    19261 aaagcctcta ctggcatcag gataacgacg cggttagcca tttaatgcgt gttgccagcg
    19321 gcctggattc actggttctt ggggagccgc agatcctcgg tcaggttaaa aaagcgtttg
    19381 tcgattcgca aaaaggtcat atgaaggcca gcgaactgga acgcatgttc cagaaatctt
    19441 tctctgtagc gaaacgcgtt cgcactgaaa cagatatcgg tgccagtgct gtgtctgtcg
    19501 cttttgcggc ttgtacgctg gcgcggcaga tctttgaatc gctctctacg gtcacagtgt
    19561 tgctggtagg cgcgggcgaa accatcgagc tggtagcgcg tcatctgcgc gaacataaag
    19621 tacagaagat gattatcgcc aaccgcactc gcgaacgtgc ccaaattctg gcagatgaag
    19681 ttggcgcgga agtgattgcc ctgagtgaga tcgacgaacg tctgcgcgaa gccgatatca
    19741 tcatcagttc caccgccagc ccgttaccga ttatcgggaa aggcatggtg gagcgcgcat
    19801 taaaaagtcg tcgtaaccaa ccaatgctgt tggtggatat tgccgttccg cgcgatgttg
    19861 agccggaagt tggcaaactg gcgaatgctt atctttatag tgtggacgat ctgcaaagca
    19921 tcatttcgca caacctggcg cagcgtaaag ccgcagcggt tgaggcggaa actattgtcg
    19981 ctcaggaaac cagcgaattt atggcgtggc tgcgagcaca aagcgccagc gaaaccattc
    20041 gcgagtatcg cagccaggca gagcacgttc gcgatgagtt aaccgccaaa gcgttagcgg
    20101 cccttgagca gggcggcgac gcgcaagcca ttatgcagga tctggcatgg aaactgacta
    20161 accgcttgat ccatgcgcca acgaaatcac ttcaacaggc cgcccgtgac ggggataacg
    20221 aacgcctgaa tattctgcgc gacagcctcg ggctggagta gcagtacatc attttctttt
    20281 tttacagggt gcatttacgc ctatgaagcc ttctatcgtt gccaaactgg aagccctgca
    20341 tgaacgccat gaagaagttc aggcgttgct gggtgacgcg caaactatcg ccgaccagga
    20401 acgttttcgc gcattatcac gcgaatatgc gcagttaagt gatgtttcgc gctgttttac
    20461 cgactggcaa caggttcagg aagatatcga aaccgcacag atgatgctcg acgatcctga
    20521 aatgcgtgag atggcgcagg atgaactgcg cgaagctaaa gaaaaaagcg agcaactgga
    20581 acagcaatta caggttctat tactaccaaa agatcctgat gacgaacgta acgccttcct
    20641 cgaagtccgt gccggaaccg gcggcgacga agcagcgctg tttgcaggcg atctgttccg
    20701 tatgtacagc cgttacgccg aagcccgccg ctggcgggta gaaatcatga gcgccagcga
    20761 gggtgaacat ggtggttata aagagatcat cgccaaaatt agcggtgatg gtgtgtatgg
    20821 tcgtctgaaa tttgaatccg gcggtcatcg cgtgcaacgt gttcctgcta cggaatcgca
    20881 gggtcgtatt catacttctg cttgtaccgt tgcggtaatg ccagaactgc ctgacgcaga
    20941 actgccggac atcaacccag cagatttacg cattgatact ttccgctcgt caggggcggg
    21001 tggtcagcac gttaacacca ccgattcggc aattcgtatt actcacttgc cgaccgggat
    21061 tgttgttgaa tgtcaggacg aacgttcaca acataaaaac aaagcgaagg cgctttcagt
    21121 gctcggtgct cgcatccacg ctgctgaaat ggcaaaacgc caacaggccg aagcgtctac
    21181 ccgtcgtaac ctgctgggga gtggcgatcg cagcgaccgt aaccgtactt acaatttccc
    21241 gcaggggcgc gttaccgatc accgcatcaa cctgacgctc taccgcctgg atgaagtgat
    21301 ggaaggtaag ctggatatgc tgattgaacc gattatccag gaacatcagg ccgaccaact
    21361 ggcggcgttg tccgagcagg aataatggaa tatcaacact ggttacgtga agcaataagc
    21421 caacttcagg cgagcgaaag cccgcggcgt gatgctgaaa tcctgctgga gcatgttacc
    21481 ggcagagggc gtacttttat tctcgccttt ggtgaaacgc agctgactga cgaacaatgt
    21541 cagcaacttg atgcgctact gacacgtcgt cgcgatggtg aacccattgc tcatttaacc
    21601 ggggtgcgag aattctggtc gttgccgtta tttgtttcgc cagcgacctt aattccgcgc
    21661 ccggatacgg agtgtctggt ggagcaggca ctggcgcggt tgcctgaaca accttgccgt
    21721 attctcgatc tcgggacggg taccggggcg attgcgcttg cgctggctag cgagcgcccg
    21781 gactgcgaaa ttatcgctgt agatcgtatg cctgatgctg tctccctggc acaacgtaat
    21841 gcccagcatc tggcgatcaa aaatatccac attctgcaaa gcgactggtt tagcgcgcta
    21901 gccgggcagc agtttgcgat gattgtcagc aatccgccgt atattgacga gcaggaccca
    21961 catcttcaac aaggcgatgt ccgctttgag ccgctcactg cgctggttgc ggcagacagt
    22021 ggaatggcag acatcgtgca tatcatcgaa cagtcgcgta acgcgctggt atccggcggc
    22081 tttctgcttc tggaacatgg ctggcagcag ggcgaagcgg tgcgacaagc atttatcctc
    22141 gcggggtatc atgacgtcga aacctgccgt gactatggtg ataacgagcg cgtaacgctc
    22201 ggccgctatt atcaatgaca agtttttcta cactgcttag tgttcatctt attagtatcg
    22261 cgctttctgt tgggctatta accttacgtt tctggctacg ttatcagaag catccacagg
    22321 catttgctcg ctggacgcgc attgtgccgc cggttgtcga tacggtgtta ttgttaagcg
    22381 gcattgcgtt gatggctaaa gcgcacatcc tgccattttc cgggcaggca caatggctga
    22441 ctgaaaagct gtttggagtt atcatttata tcgttttggg ttttattgca ctcgattatc
    22501 gtcgtatgca cagtcagcag gcgcgcatta ttgccttccc gctggcgttg gtggtgctgt
    22561 acatcatcat taaactcgcc accacaaaag taccgttact ggggtaagtc atgagatcgt
    22621 tagctgattt cgaatttaat aaagcgccat tgtgcgaagg catgatcctg gcttgcgaag
    22681 caatccgccg cgattttccc tcgcaagatg tttacgacga actggagcgt ctcgttagtc
    22741 tggcgaagga agaaatcagc cagcttctgc ctttagaaga gcagctggaa aaactgatcg
    22801 cgctgtttta cggcgactgg ggatttaaag cctcacgcgg cgtttatcgt ctttccgatg
    22861 cattatggct ggaccaggtg ttaaagaatc gacagggcag tgcggtatca ttaggtgcgg
    22921 ttttattatg ggtcgcgaat cgtctcgatt tgccgctgct gccggtgatt ttccctacgc
    22981 agctgatatt gcgcattgaa tgtccggatg gcgaaatttg gctgattaat ccttttaacg
    23041 gtgaatcgtt aagcgaacat atgctggacg gatggttaaa gggaaatatc agcccgtcgg
    23101 cggaactgtt ttatgaagac cttgatgaag ctgataacat tgaggtaatc cgcaaattgc
    23161 tggatacact caaagcctcg ttgatggaag aaaatcagat ggagctggcg ttacgcacca
    23221 gcgaagcttt attacaattc aaccctgaag atccctatga aattcgcgat cgtgggttga
    23281 tttatgcgca actggattgc gaacacgttg cgttgaacga tttaagttat ttcgttgaac
    23341 ggtgtccgga agacccgatc agcgaaatga tccgtgcgca aataaataac atcgcgcata
    23401 aacatattgt gctgcattaa ttaatcgaca ttttactcaa gattaaggcg atcctatgaa
    23461 acaaaaagtg gttagcattg gcgacatcaa cgtagcaaat gacctgccgt tcgtactgtt
    23521 tggcggtatg aacgtgctgg aatctcgcga tctggcgatg cgcatttgcg agcactacgt
    23581 aactgtgacc cagaaactgg gtatccctta cgtgttcaaa gcctcttttg acaaagccaa
    23641 ccgctcctcc atccactctt atcgtggacc gggcctggaa gaagggatga aaatcttcca
    23701 ggagttgaag cagacttttg gcgtgaaaat tatcaccgac gttcacgaac caagtcaggc
    23761 acagcccgtt gctgatgtcg tggatgtgat tcagttgccg gcgtttcttg ctcgccagac
    23821 tgacctggtt gaagccatgg cgaaaaccgg tgcggtaatt aacgtcaaga aaccacagtt
    23881 tgtcagcccg ggacagatgg gtaatatcgt tgataaattc aaagaaggcg gcaacgaaaa
    23941 agtgattctt tgcgatcgtg gtgctaactt cggctatgac aacctggttg tcgatatgct
    24001 gggcttcagc attatgaaga aagtgtctgg taactcgccg gtgattttcg acgtgaccca
    24061 cgcactgcaa tgccgcgatc cgtttggcgc agcttccggt ggtcgtcgtg ctcaggtggc
    24121 tgagctggca cgagccggta tggcggtagg tctggcgggg ctgtttattg aagcgcatcc
    24181 ggatccggaa catgcgaaat gtgatggtcc atccgcgctg ccgctggcta aactggaacc
    24241 gttcctcaag cagatgaaag cgattgatga tctggtgaaa ggtttcgaag aactggatac
    24301 cagcaagtaa tcttttttgc ttgaaaaata aagtcttagc gttctgcgtt aagacttttt
    24361 tcatgggtgc cagatacaaa aaaggccgca ggctgttacc cctgcggccg gtttcgggcg
    24421 catattgcca tcacggcagc ctgacgcccg ttttcacctt acttccggtt acgccaccag
    24481 ctgacaatcg ctgcggcaat aatccccgcc aggatcggtg ctgccaggtc gtgccagaaa
    24541 gtcatggcaa actgcgcgag cgtcatatag ccgccttgtt gtaatgacaa cattttgcgg
    24601 ctattcttga agtgtctggt ttcaagatta gcccccgttt tgttgtcagg ttttacctct
    24661 caacgtgcgg gggttttctc tttccagcaa ccaatgccac cagggataaa gcccccgcaa
    24721 cattgcgcct caccggataa ttccggcttg gtgtggatac tacgtctcaa ttcatcttca
    24781 cttcatccct gaaatgtttg caataaagag tacattccgg cttttcaaca gctgttgcag
    24841 tggtttcgtg caggttgtgg tacaggctcg caattctgat taacgagctg tccaggtagt
    24901 atgaagaaat acaataagca ggaatttatc ttatctctcc cgatttccgt catcggagga
    24961 gatataactg tcaggcaaat atcgtcatca aataggcggc aaacagtgcc agatgcgctg
    25021 cgccattgag cacgttagta cgtccggtgg agaaggagat atggcacagc actaaagagg
    25081 ccaccatcac caccatttct ggcgcaccaa gtgcaaactg caattcgtta cccgtcataa
    25141 aggcaattag cgtgacgaca ggtacggtaa gcgaaattgt tgctaacact gaaccaaaga
    25201 acagattcat cgcgcgctga acctggttgt tcaacactgc ttttaacgca cctaaacctt
    25261 ccggcgacag aatcaacagt gccaccagga agccagtaaa ggcaacaggg gcattcatgc
    25321 tgtcgagcaa tgtctccagc gagctggcgt tcattttggt gactgcaata acggcaatca
    25381 gatggataat caaccagata gcatgccaca ggctgctatg ggcagacgtt ttaccgtggt
    25441 gcgggtcgtc gtcatcactg tcatcttcgt gctcgtagac aaacaaactt tgatgcgttt
    25501 tggcctggat cagcaaaaat acgccataca ttgccgcaga aattaatgct accagtaacg
    25561 cctgaccggt tgaaaaattc gccgcaggca gagccattgg aaataccagt acgattatcg
    25621 ccagggggaa cagcgcaatt aaatactgct tgataccaaa cagattcata tattgggtgg
    25681 caaacttacg accgcccaac aataatgaaa agccaaccag cccaccggta acaatcataa
    25741 tgattgaata gagcgtatca cgcattagcg ttggcgcggc gtcgccggtt gccattaaag
    25801 ctgaaatcaa actgacttca agaataacca ctgaaaggct aagaataagc gaaccgtaag
    25861 gttctcccag gcgatgggct aatacgtccg catgacggac aacactaaag gcgctactta
    25921 aaataccaat aagcgcaaga agattgatgg caatgaccac tggtagtgtc tggctacttc
    25981 cccacaggaa cagcactacc agcaccagaa ccgggaaaat aagcgaagtc tccttgtggc
    26041 gggtttttac cgcctcttga gcatttgaca ttatggttat ccctttgcag atgaatttat
    26101 cgaaaatgta aaaaataggt aggaaaaata acagaaagtg tctggatatc ggtaacattt
    26161 tacgaatttt taccccgctg tcgatttttt actcattggg gcataaaaat aagtattacg
    26221 tttagacaat gtttgtttta gcgtttctta agaacagtct gacatgaaaa ttcttatgtt
    26281 ttggcaagag tagatattgt tgaccacact taatgttcaa ctttgtaaaa ggagtcaacg
    26341 atgccgtata aaacgaaaag cgatctgccg gaaagcgtaa agcacgttct accgtctcat
    26401 gcccaggata tctataaaga agcgttcaac agcgcatggg atcaatataa agataaagaa
    26461 gatcggcgtg atgacgccag tcgcgaagaa accgcgcata aagtggcctg ggctgctgtg
    26521 aagcatgaat atgccaaagg ggatgatgat aaatggcata aaaaatcgta aaaccggtag
    26581 cttagttaaa gctattcgtg cggtgttgcc ttgcaagtgg tccgtggatt gcatattgtt
    26641 ccgttagtgg tttcaaaatg agcggtaaaa atgtccggaa gacaccaaaa agttgtcgca
    26701 gggaagtatg cagtggcgga ggtgtaaggt gataacgcgt gatttcttga tgaatgccga
    26761 ttgtaaaacg gcatttggtg ccattgaaga atcactctta tggtcagcag aacaacgggc
    26821 ggcttcgctg gcggcgacgc tggcttgtcg acctgatgag ggaccggtgt ggatcttcgg
    26881 ttatggatcg ttgatgtgga atccggcact ggagtttacc gaatcatgca ccggtacact
    26941 ggttggatgg catcgcgcgt tttgcctgcg cctgaccgcc gggcgcggga ctgcgcacca
    27001 gccgggacgg atgcttgcac tgaaagaggg cggacgcacc acaggcgtcg cctatcgact
    27061 accagaagag acgctggagc aggaactaac cctgttgtgg aagcgcgaga tgattaccgg
    27121 ctgttatctg ccaacctggt gtcagcttga tcttgatgat ggacgcacag taaacgccat
    27181 tgtgtttatt atggacccgc gacatccaga atatgaatct gacactcgcg ctcaggtcat
    27241 cgcgccgttg attgcggcgg cgagcggtcc gctgggaacc aatgcacaat acctgttttc
    27301 actggaacag gagcttatca aactgggaat gcaggacgat gggctgaatg atttgctggt
    27361 atcggtaaaa aaactactgg cggagaatta tccggatggt gtgttaaggc cgggattcgc
    27421 ctgagtaaac ttccctcata gtggggcgtc agacgccccc ttaaacatta aaatgtgagc
    27481 actttatcgg ctgacagcgt ccattgcgcc agttccacaa gagtaccgat ttccacccca
    27541 tcaatcagag gaagtgtgct aatcccgcgc ccgtcggtac aggttttgca caatttcacc
    27601 ggtacattct gagcggtaag gatctccagc atttgctgaa tgttgtagcc ttcccctggt
    27661 ttttgcccgc gcaacccggc tgtgaccgca tcagacatca ggaacagacg cagatccaga
    27721 ttgctctcct gctctcgtaa cgcaatggcc agccgcaagc tgttaaacaa ggattcgctc
    27781 ccgtaaggtg cgccattggc aacgatcact attttttgca ttatttactc ctgtattcag
    27841 ggaattagac actcatcttc tatcttactg cttctgcagc gtctgaccaa tcggtcacat
    27901 ttttaaggat tttcctgaaa gcgcgagaaa atacgacaaa agttgccagt aatcgttatt
    27961 ctttaaggct atggtttttc attattaccg gaagttaccg acgttttgag ccgtttcgtt
    28021 cctcgcatta tcccgtttta tttactcttg cttgtagcag gcggtacagc taacgcacaa
    28081 tctaccttcg agcaaaaagc ggcaaatccc tttgataata acaatgatgg tctgccggat
    28141 ttaggcatgg ctcccgaaaa tcatgatggg gaaaaacact ttgctgaaat tgtgaaagat
    28201 ttcggcgaaa ccagtatgaa tgataacggg ctggatactg gcgagcaggc aaaagctttc
    28261 gcattgggaa aagtccgcga cgcgcttagt caacaggtta atcagcacgt agagtcctgg
    28321 ctatcaccgt ggggaaatgc cagtgttgac gtcaaagtgg ataacgaagg acatttcacc
    28381 ggcagtcgtg gaagctggtt tgtgccgtta caagataatg atcgttatct cacctggagc
    28441 cagcttggtc ttactcagca ggataatggg ttggtgagca atgtgggcgt tgggcaacgc
    28501 tgggcgcgcg gcaactggct ggtgggttat aacacttttt atgacaactt gcaggacgaa
    28561 aatcttcagc gagcgggctt tggtgccgaa gcgtggggcg aatatttgcg attatcggca
    28621 aacttttatc agccgtttgc tgcatggcat gaacagacag ccacgcagga acaacggatg
    28681 gcgcgcgggt acgacctgac agctcggatg cgcatgccgt tctatcaaca cctcaatacc
    28741 agtgtcagcc tagaacagta ttttggtgat cgtgttgatt tgtttaactc tggtacgggt
    28801 tatcacaatc ccgtcgcgtt gagtctggga ttaaattaca cccctgtgcc attagtcact
    28861 gtgacggccc agcataaaca gggggaaagt ggcgaaaatc aaaataacct cgggctgaat
    28921 cttaattacc gctttggtgt accgctcaaa aaacaacttt ctgcgggcga ggttgccgaa
    28981 agtcagtcgt tacgtggtag tcgctatgac aatccgcagc gaaataatct accgactctt
    29041 gagtaccgac agcgaaaaac gttaacggtg tttctggcga caccgccgtg ggatctaaaa
    29101 cctggcgaaa cagtgccgct gaaattacaa atccgcagtc gttacggtat tcggcaactg
    29161 atttggcagg gcgatacgca gatattaagt ttgacgccgg gcgcacaagc caacagcgcg
    29221 gagggctgga cgctgatcat gcctgactgg cagaacgggg aaggggcaag caatcactgg
    29281 cgattgtctg tggtggtgga agataaccag gggcagcgtg tctcctccaa tgagatcacg
    29341 ctaacgcttg tcgaaccgtt cgatgcattg tcaaacgacg aactgcgctg ggaaccgtaa
    29401 tcagaaaatg cgctcctgat gcacccatac cgctgcttcc acgcgagact tgagcttcat
    29461 tttcttcagc atgtgcttga cgtgcacttt tactgtgctt tcggtgatat ccaggcggcg
    29521 ggcaatcatc ttgttcggca aaccctgggc aatcagcttg agaatatcgc gctcgcgtgg
    29581 ggttaactgg ttaacatcgc gctcagtagt ggcacggtta gcgcgcaagc tggcggccag
    29641 aacaggcgtt aatgcttcgc ttaataccat ttcgccagca gctgcctgat gcaatgcttt
    29701 cagcagatct tccggttcca tatcttttaa cagatagcca tccgcgccgc gtttcagtgc
    29761 ggtgaccaca tcttcttcat ggttagagac gctgaatacc acaatgcgcc ctgagaggga
    29821 cttttcgcgc agtttatcca gcgtttccag accgttcatg ccgggcatat tgagatctaa
    29881 caggatcaga tcgggatcaa gagattccgc cagttcaata ccctgttcgc cattactcgc
    29941 ttcgccaacc acggtgatat ctggtgccat actgataagc tgttttacgc cagttcgcag
    30001 catcgggtga tcgtcaatca gcaggatagt agccggttcc tgattactca tgggtatctc
    30061 cttggacgtc tgtgaaagtt ttttcgggaa taaaggtgac caccacttcg gtgccacctg
    30121 attcacgacg gcggacgcgg caatcgcctc gtaaactttg cgcacggtcg cgcattatta
    30181 tcatgccgta gtgattgctg cggatggcat tttcaggcac gccacagccg ttatcctgga
    30241 cggtcagttt gacctgatta tcgttttgcg ccaccgtcac cacgacctca ctcgcttgcg
    30301 aatgtttgag ggcgttactt aatgcctcac gggcaatttg caacaagtgg attgcctgat
    30361 gtgaaggcac cagacgaggc ggcaattgat aatccagctt caccgggaag ccaaatttgg
    30421 cgctgtactc ttcgcaactc gcttccagcg ccggacgtaa tccaggctcg gtgagctgca
    30481 agcggaatgt ggtgagcaat tcacgcaact gcgcccagga tgcattcagt tcgttacgga
    30541 tctgacttaa cagttcgcgg ctgctttctg gcagcgcatc gccctgcatc tgtaaacaac
    30601 tcacctgcat cttcatgcaa gagagagatt gggcaataga atcatgcagt tcgcgcgcaa
    30661 tggtggcacg ctcttccatc acgatcaact gttgctgacg ttcctgatgg cgatccagcg
    30721 ccagcgtggc ggtaagttgt tcaaccaggg tatccaccag ttgttgttgg tcatggctaa
    30781 gatgacgccc ctgcggcagg gtcgccagca aaataccgta ctgcgtatga gagtcagcca
    30841 gccgccactt cagggtcgtg ccgcgatcac caacgggtaa tacgccgcgc gggcagagct
    30901 ggcagccttt atcatcacaa gtcatatctg gctggcaggt aaactcctga tgattctctt
    30961 catcatcagt gtcatacacc cgcagttcga tatcacgtag cagggttaaa ttctgtaagc
    31021 cgttgagtac cggtgacagg cgttcacaca gcggggcgcg ggaatgcaaa cggcggttag
    31081 cctgccataa aaaagagagg atctgatttt tatgctccag cccggcggtt ttctcctgaa
    31141 cccgctgctc aagtacggca taactttcgg ccagttctgc agacatattg ttcaacgcag
    31201 ttccaagcat cgccatttcg ttgcgcccgc tgatgtttgc gcgttgggta aaatcgcgat
    31261 ggctgacggc actcgccatt gccagcagtt gccgccacgg ttgtagcagt cgcgcccgca
    31321 accagataat agtgaacacc agtaaaagtg ccataaatac cgccattacc cgatggacca
    31381 gtaccactgt ctcaatacgc atttccgtgg tgcggtcaaa accagatacc agctgatcaa
    31441 gcccggcaac aaactggctg acatccgctg acaccgtttc tcggttttgt gcacgcatca
    31501 gcgcagggat cagttcatta cgccagtaat cttgtaagcc ctgtaattgc gccagttgtc
    31561 cgtctcgttc tgctgctcga gtcaactcgg cgctaaatgc cgtttgttcc atctctttaa
    31621 ttaagggctt gtctttctcg cttaatggca ctgccgccaa cagacggtaa ctttgcatgc
    31681 gcagcgatcc cgctttgttg atcgcatggg cgctgccctg aacgccttgc accagccagc
    31741 cagaaaccgc catccctgcc agtccaatag cagtagaaag caacacaata agcgcaacct
    31801 gattaaccag ggtgagcgga gagagacaac gtttaagcat gtaaacccct tccttcaggc
    31861 tttaaatgag caataacctt aatgaatgtg acgatatatt ctggaatggc agtattctcg
    31921 gctattggct gaagtatacc catagccgga aagagttact ccttatttgc cgtgtggtta
    31981 gtcgctttac atcggtaagg gtagggattc tacagcaccg tgaaaaatct cataattttt
    32041 atgaaatcac tgtactcact atgggtaatg ataaatatca atgatagata aagttatctt
    32101 atcgtttgat ttacatcaaa ttgcctttag ctccagacac taaggtggca gacatcgaaa
    32161 cgagtatcag aggtgtctat gagtcactca tctgcctccg aaagggctac tggagctgtc
    32221 ataacagatt ggcgaccgga agatcctgcg ttctggcaac aacgcggtca acgtattgcc
    32281 agccgcaacc tgtggatttc cgttccctgt ctgctgctgg cgttttgcgt atggatgttg
    32341 ttcagcgctg ttgcggtgaa cttaccgaaa gtcggtttta attttacgac cgatcagcta
    32401 tttatgttga ctgcgctgcc ttcggtttct ggcgcgttat tacgtgttcc atactccttt
    32461 atggttccta tcttcggtgg tcgtcgctgg acggcgttca gcaccggtat tctgattatt
    32521 ccttgcgtct ggctgggttt tgccgtgcag gatacctcca cgccttatag cgtcttcatc
    32581 atcatctctc tgctatgcgg ctttgctggc gcgaacttcg catccagtat ggcaaacatc
    32641 agcttcttct ttccgaaaca gaagcagggt ggcgcgctgg gtctgaatgg tggtctggga
    32701 aacatgggcg tcagcgtcat gcagttggtt gctccgctgg tggtatcact gtcgattttc
    32761 gcagtatttg gtagccaggg cgtcaaacag ccggatggga ctgagctgta tctggcgaat
    32821 gcgtcctgga tatgggtgcc gttccttgcc atcttcacca ttgcggcgtg gtttggcatg
    32881 aacgatcttg ctacctcgaa agcctccatc aaggagcagt tgccggtact caaacggggt
    32941 catctgtgga ttatgagcct gctgtatctg gcaaccttcg gctccttcat cggcttctcc
    33001 gcgggctttg cgatgctgtc aaaaacgcag ttcccggatg ttcagattct gcaatacgct
    33061 ttcttcgggc cgtttattgg tgcgctggcg cgttctgcag gtggtgcatt atctgaccgt
    33121 ctgggcggaa ctcgtgtcac gctggtgaac tttattctga tggcgatttt cagcggcctg
    33181 ctgttcctga ccttaccgac tgacgggcag ggcggaagct tcatggcgtt cttcgcggtc
    33241 ttcctggcgc tgttcctgac agctgggctg ggtagtggtt ccactttcca gatgatttca
    33301 gtgatcttcc gtaaactgac aatggatcgc gtgaaagcag aagggggttc tgacgaacgt
    33361 gcgatgcgtg aagcggcaac cggcacggca gcggcgctgg gtttcatctc tgcgattggc
    33421 gcgattggtg gcttctttat cccgaaagcg tttggtagct cgctggcatt aacgggttcg
    33481 ccagtaggtg caatgaaagt atttttgatt ttctatatcg cctgcgtagt gattacttgg
    33541 gcggtatatg gtcggcattc taaaaaataa atcttaatat ttgattatcc tttgcgcggc
    33601 atgatgccgc gcttttttta tgcgtcattt agttacaaca tattagtgt
//
LOCUS       DAZSNO010000029        33225 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZSNO010000029 DAZSNO010000000
VERSION     DAZSNO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33225
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.029"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..190
                     /locus_tag="ACHWN2_001339"
     CDS             <1..190
                     /locus_tag="ACHWN2_001339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8930941.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACHWN2_001340"
     CDS             complement(156..1391)
                     /locus_tag="ACHWN2_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR8930942.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACHWN2_001341"
     tRNA            complement(1538..1614)
                     /locus_tag="ACHWN2_001341"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="ACHWN2_001342"
     tRNA            complement(1657..1743)
                     /locus_tag="ACHWN2_001342"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="ACHWN2_001343"
     tRNA            complement(1764..1839)
                     /locus_tag="ACHWN2_001343"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="ACHWN2_001344"
     tRNA            complement(1898..1974)
                     /locus_tag="ACHWN2_001344"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="ACHWN2_001345"
     CDS             complement(2077..3462)
                     /locus_tag="ACHWN2_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8930943.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHWN2_001346"
     CDS             complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACHWN2_001346"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR8930944.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHWN2_001347"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACHWN2_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR8930945.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHWN2_001348"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACHWN2_001348"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR8930946.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHWN2_001349"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACHWN2_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR8930947.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHWN2_001350"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACHWN2_001350"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR8930948.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHWN2_001351"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACHWN2_001351"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR8930949.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHWN2_001352"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="ACHWN2_001352"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR8930950.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHWN2_001353"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="ACHWN2_001353"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR8930951.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHWN2_001354"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="ACHWN2_001354"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR8930952.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHWN2_001355"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="ACHWN2_001355"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR8930953.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHWN2_001356"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="ACHWN2_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR8930954.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHWN2_001357"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="ACHWN2_001357"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR8930955.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHWN2_001358"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="ACHWN2_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR8930956.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHWN2_001359"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="ACHWN2_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR8930957.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHWN2_001360"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="ACHWN2_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR8930958.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHWN2_001361"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="ACHWN2_001361"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR8930959.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="ACHWN2_001362"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="ACHWN2_001362"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR8930960.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHWN2_001363"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="ACHWN2_001363"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR8930961.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHWN2_001364"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="ACHWN2_001364"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR8930962.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHWN2_001365"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="ACHWN2_001365"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR8930963.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHWN2_001366"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="ACHWN2_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR8930964.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHWN2_001367"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="ACHWN2_001367"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR8930965.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHWN2_001368"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="ACHWN2_001368"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR8930966.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHWN2_001369"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="ACHWN2_001369"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR8930967.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHWN2_001370"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="ACHWN2_001370"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR8930968.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHWN2_001371"
     CDS             complement(31267..32913)
                     /gene="ilvG"
                     /locus_tag="ACHWN2_001371"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR8930969.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHWN2_001372"
     CDS             complement(32916..32966)
                     /gene="ilvX"
                     /locus_tag="ACHWN2_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR8930970.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHWN2_001373"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="ACHWN2_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR8930971.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8026 a         9209 c         8399 g         7591 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccagacag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatt
//
LOCUS       DAZSNO010000030        33179 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZSNO010000030 DAZSNO010000000
VERSION     DAZSNO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33179
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.030"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(144..284)
                     /gene="yjjY"
                     /locus_tag="ACHWN2_001374"
     CDS             complement(144..284)
                     /gene="yjjY"
                     /locus_tag="ACHWN2_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR8930972.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            380..1096
                     /gene="arcA"
                     /locus_tag="ACHWN2_001375"
     CDS             380..1096
                     /gene="arcA"
                     /locus_tag="ACHWN2_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR8930973.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1155..2507)
                     /gene="creD"
                     /locus_tag="ACHWN2_001376"
     CDS             complement(1155..2507)
                     /gene="creD"
                     /locus_tag="ACHWN2_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR8930974.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2565..3989)
                     /gene="creC"
                     /locus_tag="ACHWN2_001377"
     CDS             complement(2565..3989)
                     /gene="creC"
                     /locus_tag="ACHWN2_001377"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR8930975.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3989..4678)
                     /gene="creB"
                     /locus_tag="ACHWN2_001378"
     CDS             complement(3989..4678)
                     /gene="creB"
                     /locus_tag="ACHWN2_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR8930976.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4691..5164)
                     /gene="creA"
                     /locus_tag="ACHWN2_001379"
     CDS             complement(4691..5164)
                     /gene="creA"
                     /locus_tag="ACHWN2_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR8930977.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5375..6244
                     /gene="robA"
                     /locus_tag="ACHWN2_001380"
     CDS             5375..6244
                     /gene="robA"
                     /locus_tag="ACHWN2_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR8930978.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6241..6888)
                     /gene="gpmB"
                     /locus_tag="ACHWN2_001381"
     CDS             complement(6241..6888)
                     /gene="gpmB"
                     /locus_tag="ACHWN2_001381"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR8930979.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6940..7460
                     /gene="yjjX"
                     /locus_tag="ACHWN2_001382"
                     /pseudo
     CDS             6940..7460
                     /gene="yjjX"
                     /locus_tag="ACHWN2_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(7545..7871)
                     /gene="trpR"
                     /locus_tag="ACHWN2_001383"
     CDS             complement(7545..7871)
                     /gene="trpR"
                     /locus_tag="ACHWN2_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR8930980.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7961..9898)
                     /gene="sltY"
                     /locus_tag="ACHWN2_001384"
     CDS             complement(7961..9898)
                     /gene="sltY"
                     /locus_tag="ACHWN2_001384"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR8930981.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10109..11776
                     /gene="ettA"
                     /locus_tag="ACHWN2_001385"
     CDS             10109..11776
                     /gene="ettA"
                     /locus_tag="ACHWN2_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR8930982.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11883..12050)
                     /gene="nadS"
                     /locus_tag="ACHWN2_001386"
     CDS             complement(11883..12050)
                     /gene="nadS"
                     /locus_tag="ACHWN2_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFR8930983.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(12084..13316)
                     /gene="nadR"
                     /locus_tag="ACHWN2_001387"
     CDS             complement(12084..13316)
                     /gene="nadR"
                     /locus_tag="ACHWN2_001387"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR8930984.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(13337..14719)
                     /gene="radA"
                     /locus_tag="ACHWN2_001388"
     CDS             complement(13337..14719)
                     /gene="radA"
                     /locus_tag="ACHWN2_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR8930985.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14768..15736)
                     /gene="serB"
                     /locus_tag="ACHWN2_001389"
     CDS             complement(14768..15736)
                     /gene="serB"
                     /locus_tag="ACHWN2_001389"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR8930986.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15842..16486
                     /locus_tag="ACHWN2_001390"
     CDS             15842..16486
                     /locus_tag="ACHWN2_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR8930987.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16514..17529
                     /gene="lplA"
                     /locus_tag="ACHWN2_001391"
                     /pseudo
     CDS             16514..17529
                     /gene="lplA"
                     /locus_tag="ACHWN2_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            17561..17824
                     /locus_tag="ACHWN2_001392"
     CDS             17561..17824
                     /locus_tag="ACHWN2_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8930988.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(17985..18704)
                     /gene="deoD"
                     /locus_tag="ACHWN2_001393"
     CDS             complement(17985..18704)
                     /gene="deoD"
                     /locus_tag="ACHWN2_001393"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR8930989.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18761..19984)
                     /gene="deoB"
                     /locus_tag="ACHWN2_001394"
     CDS             complement(18761..19984)
                     /gene="deoB"
                     /locus_tag="ACHWN2_001394"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR8930990.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20036..21358)
                     /gene="deoA"
                     /locus_tag="ACHWN2_001395"
     CDS             complement(20036..21358)
                     /gene="deoA"
                     /locus_tag="ACHWN2_001395"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR8930991.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(21485..22264)
                     /gene="deoC"
                     /locus_tag="ACHWN2_001396"
     CDS             complement(21485..22264)
                     /gene="deoC"
                     /locus_tag="ACHWN2_001396"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR8930992.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22522..24063
                     /locus_tag="ACHWN2_001397"
     CDS             22522..24063
                     /locus_tag="ACHWN2_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR8930993.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            24035..24898
                     /locus_tag="ACHWN2_001398"
     CDS             24035..24898
                     /locus_tag="ACHWN2_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR8930994.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(25112..25894)
                     /locus_tag="ACHWN2_001399"
     CDS             complement(25112..25894)
                     /locus_tag="ACHWN2_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8930995.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(25891..26964)
                     /locus_tag="ACHWN2_001400"
     CDS             complement(25891..26964)
                     /locus_tag="ACHWN2_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR8930996.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27086..27247)
                     /locus_tag="ACHWN2_001401"
     CDS             complement(27086..27247)
                     /locus_tag="ACHWN2_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR8930997.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27364..27969)
                     /gene="osmY"
                     /locus_tag="ACHWN2_001402"
     CDS             complement(27364..27969)
                     /gene="osmY"
                     /locus_tag="ACHWN2_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR8930998.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28362..29951)
                     /gene="prfC"
                     /locus_tag="ACHWN2_001403"
     CDS             complement(28362..29951)
                     /gene="prfC"
                     /locus_tag="ACHWN2_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR8930999.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30042..30719)
                     /gene="yjjG"
                     /locus_tag="ACHWN2_001404"
     CDS             complement(30042..30719)
                     /gene="yjjG"
                     /locus_tag="ACHWN2_001404"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR8931000.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(30734..31180)
                     /gene="rimI"
                     /locus_tag="ACHWN2_001405"
     CDS             complement(30734..31180)
                     /gene="rimI"
                     /locus_tag="ACHWN2_001405"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR8931001.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31149..31562)
                     /gene="holD"
                     /locus_tag="ACHWN2_001406"
     CDS             complement(31149..31562)
                     /gene="holD"
                     /locus_tag="ACHWN2_001406"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR8931002.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            31665..32696
                     /gene="rsmC"
                     /locus_tag="ACHWN2_001407"
     CDS             31665..32696
                     /gene="rsmC"
                     /locus_tag="ACHWN2_001407"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR8931003.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            32973..33059
                     /locus_tag="ACHWN2_001408"
     tRNA            32973..33059
                     /locus_tag="ACHWN2_001408"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33007..33009,aa:Leu,seq:cag)
     gene            33088..33174
                     /locus_tag="ACHWN2_001409"
     tRNA            33088..33174
                     /locus_tag="ACHWN2_001409"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33122..33124,aa:Leu,seq:cag)
BASE COUNT         7817 a         9129 c         8475 g         7758 t
ORIGIN      
        1 caactagttg gattattaaa ataatgtgac gaaagctagc atttagatac gatgatttca
       61 tcaaactgtt aacgtgctac aattgaactt gatatatgtc aacgaagcgt agttttattg
      121 ggtgtccggc ccctcttagc ttgttatgtt gctgttaaaa tggttaggat gacagccgtt
      181 tttgacactg tcgggtcctg agggaaagta cccacgacca agctaatgat gttgttgacg
      241 ttgatggaaa gtgcatcaag aacgcaatta cgtactttag tcatgttacg ccgatcatgt
      301 taatttgcag catgcatcag gcaggtcagg gacttttgta cttcctgttt cgatttagtt
      361 ggcaatttag gtagcaaaca tgcagacccc gcacattctt atcgttgaag acgagttggt
      421 aacacgcaac acgttgaaaa gtattttcga agcggaaggc tatgatgttt tcgaagcgac
      481 agatggcgcg gaaatgcatc agatcctctc tgaatatgac atcaacctgg tgatcatgga
      541 tatcaatctg ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa
      601 tgttgcgttg atgttcctga ctggccgtga caacgaagtc gataaaattc tcggcctcga
      661 aatcggtgca gatgactaca tcaccaaacc gttcaacccg cgtgaactga cgattcgtgc
      721 acgcaacctg ctgtcccgta ccatgaatct gggtactgtc agcgaagaac gtcgtagcgt
      781 tgaaagctac aagttcaatg gttgggaact ggacatcaac agccgttcgt tgatcggccc
      841 tgatggcgag cagtacaagc tgccgcgcag cgagttccgc gccatgcttc acttctgtga
      901 aaacccaggc aaaattcagt ctcgtgctga actgctgaag aaaatgaccg gccgtgagct
      961 gaaaccgcac gaccgtactg tagacgtgac gatccgccgt attcgtaaac atttcgaatc
     1021 tacgccggat acgccggaaa tcatcgccac cattcacggt gaaggttatc gcttctgcgg
     1081 tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa cggcgctttt tagcgccgtt
     1141 tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg
     1201 gctttcattt tcggcagtga aaacgcatac cagtctatat tacgagtcac aaacatcatg
     1261 ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc ggcagagttg
     1321 agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg
     1381 cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg
     1441 gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa
     1501 aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg
     1561 agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta
     1621 gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg
     1681 gggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca
     1741 aaccagctgc tttgccattg cgcctgaaaa cctgactcgc tagcttcccg tttggctggt
     1801 agaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta
     1861 cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagtgc catattcagc
     1921 tgcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct
     1981 tgctctaacc cggtaccggg ttcaatggtc agtgccgttc cgttaacttc aggcgctttc
     2041 accacaccaa taccacgcgc atccccgacg ctaatcacaa taaagggctt gcctaagatg
     2101 atatttggcg cgttgagctc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa
     2161 tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg
     2221 ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg
     2281 gttttatcct cttcctgcac cgtataaagc tcggtcacag gaatagcgat aagcggccca
     2341 acgagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa
     2401 tcggcacgtt cgacaatcac ttgccgaatc agcattatcg gaatcaacaa cagcaatact
     2461 gcaccaaaca aggtggtcat tttccagaac aggggggatt tcaacatagt cttctccttt
     2521 gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg
     2581 tgaagtcgta gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg
     2641 ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca
     2701 tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata
     2761 ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc
     2821 gttatgcaac cgctctcggg agtaaaatcg atggcgttat ccagtaaatt ccccagcgcc
     2881 tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcataac atgcaaagtg
     2941 atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcgaaa taatgccgcc
     3001 acatcaacag cagtcagaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac
     3061 gtttccacca gcgcctgcat tcgcgcattt tgtgtcagaa tgttgtcagt aaaacgagcc
     3121 actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt
     3181 gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc
     3241 agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga
     3301 tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca
     3361 atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcaat ccccaacaaa
     3421 atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg
     3481 ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgcccataat cggcgcggca
     3541 acatacatca ccgaactttc gggatcggca ggattttgca acgtgctgcg cgcaccatac
     3601 tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgtcc aacggcttta
     3661 tttgccgaat cgaacaatac tttgccatgc gcatcggtca tatagacatg atattcattg
     3721 cgcactttgt taatgccacc aatattggcg cgaaacgggc gatgttgtag ctgattaaac
     3781 gcctgcgcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc
     3841 gcaagtaacg ttgccgtgtc gatcaacgtc ccctccgttg ctcttcgcac gcccggttta
     3901 acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag
     3961 cccagcagca accgcatgcc gatacgcatt acaagcccct caggctatat cccatgccgc
     4021 gatgagtatt aatcggtgaa agatcggggt tgatggcgcg cagcttggca cgcagcgttt
     4081 taatgtggat atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca
     4141 ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa
     4201 actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat
     4261 tcaattcaaa atgaccaata cggatgacgg gagacggcgt cgagaacttc ttcacccgac
     4321 gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt
     4381 cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga
     4441 acagtacagg taacgccgga tggagcgcca gtaactggcg gcacaattca aagccactga
     4501 tatcaggcaa accaacgtcg agaatcatga cgtcgggaac ctgctggcga gctttatcca
     4561 gcaccggcaa acctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga
     4621 ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa
     4681 atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg
     4741 aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat
     4801 aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct
     4861 cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac
     4921 aagaaatggc cgcatcggag gtatcttctg ccaggcccaa tcccccttta ataccaccgg
     4981 ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca
     5041 caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt
     5101 ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt
     5161 tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg taaataacat
     5221 attgattaag aacaaaatct caattctttt atttccgtca agccctaaaa catactctac
     5281 taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt
     5341 caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga
     5401 ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc
     5461 gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc
     5521 tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac
     5581 tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac
     5641 ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg
     5701 gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa
     5761 atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct
     5821 ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg
     5881 caacgcgccg accattccgc cggtgctcta cggcctgaat gaaacgcgtc cgagtcagga
     5941 taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg
     6001 ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta
     6061 tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc
     6121 aatgctcaat ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga
     6181 cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg
     6241 ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc
     6301 aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca
     6361 gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag
     6421 acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg
     6481 gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg
     6541 aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt
     6601 cagagaatcg atatgtcttt tttccagcac acccatgttt aattcacgca ggcgagaatc
     6661 aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc
     6721 taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat
     6781 cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc
     6841 ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg
     6901 tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt tgtctgtgcg
     6961 accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa
     7021 ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca accctttggc
     7081 agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca
     7141 gaggctgatt tttgggtggc gattgaagct ggcattgatg gcgacagcac tttcagttgg
     7201 gtggtgattg aaaacgccag ccagcgcggc gaggcgcgtt cggcaacttt gccgttacct
     7261 gcggtgattc tggaaaaagt tcgtgaaggc gaggcgcttg gtcccgtgag tcgcgttata
     7321 ccggtattga tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc gccgggaaac
     7381 tcactcgcgc cagcgtctat caccaggcgg tgatcctggc gctgagtccg tttcacaatg
     7441 ccgtgtatca ggcattgtag gacggataag gcgttcacgc cgcatccgac attcggtgca
     7501 cgatgcctga tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc gcttttcagc
     7561 aacacctctt ccagccactg acgcagctcc acgggcgcgg ctttcaggct gttagatcca
     7621 cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta actcacgctg actcatttcg
     7681 ccacgcaaca gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc
     7741 gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg
     7801 tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat
     7861 tgttgggcca taatatgtcg ccattgttag cgggggaagc aaaatgactc ccccggttgt
     7921 actcgctaaa gagtacgata gcatatcata aacgtgcgga tcagtaacga cgtccccatt
     7981 ccgtggcgct catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcatcataag
     8041 ccagcacgtt cttcacataa ccgcgcgtct cggagaacgg aatactctcg acaaatgcca
     8101 cagcgtcgat acgcccggcg ctgttgccaa gccaggttcg cacccgtcca ggtccggcgt
     8161 tataagctgc tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcaggt
     8221 aactggtgcc aatgttgatg ttcgtttccg gatccagcaa ttgcccaggg ctgctataac
     8281 caggaataga gaacatcttc accgtatggg tcgctgtacc aggcataatc tgcatcaggc
     8341 cgctggcccc taccggtgat ttcactttcg gattccaggc gctctcctga cgggcaatcg
     8401 ccatcgcata gctttgcggg atctctttcc cactggtgta acgtttgaaa agatcgttgt
     8461 aagccagcgg gaatcgctct tccagatgat cccaaagctt cccggcgatc gttgcctgaa
     8521 cgctaagatc ccaccattgg ttattgaaag cataccgagc cagttgagcc tgctctgttt
     8581 ttgacttgct cttcaccaga ttggcccact cgctacgcgc ggtattatcg agattccagt
     8641 acatcaactc gcgcacgcgc gccatctccg gcccctgagt cagggtgctg tcaacattct
     8701 gcggcgcttt atcaatcttc agctcatact cttcaccgat gcgttgtgct gcaaccatcg
     8761 ggtagaaacc acgctgttgt atgagttgat gcaaaatctc tttcgcttca gcttcacgtc
     8821 cgcgttccag caataaatcc gcctgccagt aacgccattc atctttctct tttgcttcca
     8881 tcggcagacg cgccagccag gtattcaggc cgcgacgatc gccggtgcca agcgccattc
     8941 gcacacggcg ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca tcgcgccatt
     9001 tcgcctgctc atcggtgaca tcattgccca tcaaacgcca ggcgacgata tcgcgcagct
     9061 cctgaatctg atcttcatta agctgctgcg cctgggcaag cgacgggatc atcagccgtg
     9121 cattttcggc atcctgccgc gccacactgg caaacgccac cgccgccatt tgacgggtaa
     9181 aatcggtcgc gccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg
     9241 aaatgattgc cgaggcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta
     9301 ccaggcctgt gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat
     9361 cttgtttacc tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct
     9421 tgccggttag ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc
     9481 atttcgcata gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg
     9541 ctaacaagcc acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg
     9601 tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt
     9661 gattcatcag gtcatcggtg atctggcggt attccaggta gggataaagc ggataatcct
     9721 tcagtccagg catcatttgt tcgaccacat ccatctgtcg attatcccag gcctgcttga
     9781 tttgcgcgta acggctacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg
     9841 tcagcagaca gacaccggca gccaacagcc gccaggtaac ttgtttggct ttttccacaa
     9901 gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct
     9961 aaccagtcat tgccgtttac gccacgttac ggacactttt ttacttttac tgcgaaggtg
    10021 atcgaccaac tgggattagg cggggaaaca ggctacactt cgcctttgaa aactcatcat
    10081 cacgataaac atagaggcga agtccaacgt ggctcaattc gtttatacca tgcatcgtgt
    10141 cggcaaagtt gttccgccga aacgtcatat tttgaaaaac atctctctga gtttcttccc
    10201 tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt aagtccaccc tgctgcgcat
    10261 tatggcgggc attgataaag acatcgaagg tgaagcgcgt ccgcagccag acatcaagat
    10321 tggttatctg ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg agtccattga
    10381 agaagcggtt tcagaagtgg ttaacgccct gaaacgcctg gatgaagtgt atgcgctgta
    10441 cgccgatccg gatgccgatt ttgacaagct ggccgctgaa caaggccgtc tggaagagat
    10501 cattcaggct cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg cggatgcgct
    10561 gcgtctgcca gattgggacg cgaaaatcgc taacctctcc ggtggtgagc gtcgtcgcgt
    10621 ggcgttgtgc cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg aaccgaccaa
    10681 ccacctggat gccgaatccg tggcgtggct ggaacgcttc ctgcacgact tcgaaggcac
    10741 cgtggtggcg attactcacg accgttactt cctcgataac gttgcaggct ggatcctcga
    10801 actggaccgc ggtgaaggaa ttccgtggga aggtaactac tcctcctggc tggagcagaa
    10861 agatcagcgt ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt cgatcgagaa
    10921 agagctggag tgggtacgtc agggaactaa aggccgccag tcgaaaggta aagcacgtct
    10981 ggcacgcttt gaagagctga acagcaccga ataccagaaa cgtaacgaaa ccaacgaact
    11041 gtttattcca cctggaccgc gtctgggcga taaagtgctg gaagtcagca acctgcgtaa
    11101 atcctacggc gatcgcctgc tgattgatga cctgagcttc tcgatcccga aaggggcgat
    11161 cgtcgggatc atcggtccga acggcgcggg taaatcgacc ctgttccgta tgatctccgg
    11221 tcaggaacag ccggacagcg gcaccatcac tttgggtgaa acggtgaaac tggcatcggt
    11281 tgatcagttc cgtgactcaa tggataacag caaaaccgtt tgggaagaag tttccggcgg
    11341 gctggatatt atgaagatcg gcaacaccga gatgccaagc cgcgcctacg ttggccgctt
    11401 taactttaaa ggggttgatc agggtaaacg tgttggcgaa ctttccggtg gtgagcgcgg
    11461 tcgtctgcat ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc tcgacgaacc
    11521 aaccaacgac ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc tggagttccc
    11581 gggctgtgcg atggttatct cgcacgaccg ttggttcctc gaccgtatcg ccacgcacat
    11641 cctggactac caggatgaag gtaaagttga gttcttcgaa ggtaacttta ctgagtacga
    11701 agagtacaaa aaacgcacgc tgggcgcaga cgcgctggag ccgaagcgta tcaagtacaa
    11761 gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgc gaacgcctta tccggcctac
    11821 aaaaccagca atttcaatac gttgtaaaac tgtaggcctg ataagacgcg gtaagcgtcg
    11881 catcaggcat tgagcaccga atgccggatg cggcgtgaac accttttccg gtctacaaca
    11941 gccttagcat cctcaatttc gtggcgggtc acccgtgcag gttttttcaa accttgttta
    12001 atctcgacag cctcttccag agaggttttc aactcatcaa aaaaactcat tttatacctc
    12061 gcctttgagc agtttcgtca cggttatcct tgctccccca tcatctcccg caccagctcc
    12121 acgcagcgca ggaaacggct gtcgtaatcg tcctcttcaa cccgcacgaa ttcgatattg
    12181 ttctcttcca gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg
    12241 aggctgcgta aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg
    12301 aaacggtatt catcaatcag cgcctgcacg aacggatgct cacgcccttc gtactttttg
    12361 cagaacgcct gggtagtgac aaaatcggtg tcaataaacg ccactttatt ggcgtatttc
    12421 actgcaaaat caatgtattg tgcgtgaccc aacgcgattt tatcgtagtc ggaatactgc
    12481 aatgcgatct catcaccgcc gaggtgtgaa aagacataat cgcggccata ttcccacgca
    12541 ctggtggtgt tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg
    12601 ccgccaagga tcgccacggt acgcacaaaa aacggcttca cttcggtagg aatatattcc
    12661 cagtagcgga acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc
    12721 ggatcgacca gcaccgtctc gatccccaga tgttccatat actgtggcgc atcggcttct
    12781 tccgaggtgt agatcaggtc cggctggatc cccttttcag ccataaactt tttgatgccg
    12841 ttgctccaca catcccagcc atgcggatac ggctccatgc cctcttcgtt gaaagcatga
    12901 atacggatat ttttctgata tttaaaggtt tgcaatagcc agcgcagacg atccggcacc
    12961 gtgggctgct gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa
    13021 cccataatga tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt
    13081 ccggtatgca gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgccgggga
    13141 aattcaagcc ccaaaaaacg gtgcaatgcc tccagctttt gcgcgctggg gcttttaatt
    13201 ttggcattca gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc
    13261 tgtagcgtgc agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct
    13321 ccctccgtat ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa
    13381 cgccaaaaat ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg
    13441 cccggcgaaa accgtgtttc gccgcttcag aaatccgctc ctggccactg ggcaccgggc
    13501 ggatctcccc tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt
    13561 cacgcaggct ggaaaccatc gccagcagta acgctaagtc ggcgctggtt tcggttacct
    13621 tcacgccgcc gaccacgttc acaaacacat cctgatcggc catttgcaga ccaccgtgac
    13681 ggtgcaagac agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc
    13741 gcggattcgc cattatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg
    13801 ttccttccca caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga
    13861 aaattgccga agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca
    13921 gctcattcac cgcgccgaag cggtttttat ggctgcgtaa ggtgcgaaaa cgggagtcgg
    13981 catcgccatc cagaagcacc gaacagtcga tacagtgttc cagcactttc gggccagcca
    14041 gcgaaccatc tttggttacg tgtccaacca tgacaatcgc cacaccgcgc gttttggcga
    14101 aacgcgtcag ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta
    14161 catccgccat atgcatcacc tggatcgagt caattaccat cagcttcggt tgctcttctt
    14221 cggcaatcag gcagatctgc tcgatgctgg tttccgacaa catattgaga ttgtcagtcg
    14281 gcaggccaag gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccggtgacat
    14341 acagcgtttt catctgctgg gccagtttgc acagcgtttg cagcaacagc gtggatttcc
    14401 ccgcaccagg gttaccgcca atcagaatgg cacttcctgg caccacgccg cctcctagta
    14461 cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg
    14521 agagtttctg gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac
    14581 gcgccaccgt tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg
    14641 cactgcactg cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc
    14701 gttttggagc ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatgacggg
    14761 caggcaatta cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag
    14821 cgtgacggat ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc
    14881 ccagccctgc tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct
    14941 gcgccagcgg gatttcatac tcctgcgcga ggcgagtcag agttttcgct ttgtactgcg
    15001 cgtctacgat gtcgccgatc acattgccgg taaatttacc gtccatgatc tccagttcat
    15061 tggctaccac ggctgtcagg cgcagcttgt cgcgcaggta ttcagcaaag aaagtaaagc
    15121 cgccggaggc aatcgccact ttccagccca gcgtttccag cttgagcacc agctgcgtta
    15181 agcctggcat cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt
    15241 tcagcgtcgc cacgcggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc
    15301 gttcggttac ctccgccacc atctcgcccg ttccggccag cttggcaatt tcatcaatac
    15361 attcaatctg gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt
    15421 gcgggatttt ccccagcggg gcgacatcca gctgcgcttc gtgggccagg cgtgtagccc
    15481 gtgcggtgag tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa
    15541 caatcaccat cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc
    15601 cacgaccata cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat
    15661 caccacttaa tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc
    15721 aggtaatgtt aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg
    15781 aggctacctt gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa
    15841 gatggctcgc acaaaactga aattccggct acatcgggca gtgattgtcc tgttctgtct
    15901 tgccttgtta gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca
    15961 gcgtaatcca cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt
    16021 tgcaccgctg atgcgtaccg actcaccgga tgaaaaacgc attcaggcga tcctcgatca
    16081 gttaacagat gaaagccgta tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat
    16141 cgcacgttct ggcgaaagcg tcgaagtgcg cgatcggctg gcgctcgacg gtaaaaaagc
    16201 aggcggctat tttaaccagc agattgtcga gccaattgcg ggtaaaaacg gaccgctcgg
    16261 ctatctgcgc ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa
    16321 caccactaac attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac
    16381 ccgcacgctg ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc
    16441 cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag
    16501 aaaggaaatc gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt
    16561 taacctggcg gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt
    16621 tctctggcgc aatgccgaca cggtagtaat tggtcgcgcg cagaacccgt ggaaagagtg
    16681 taatacccgg cggatggaag aagataacgt ccgcctggcg cggcgcagtg gcggtggcgg
    16741 cgcggtgttc cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta
    16801 cgataaaact atctccacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc
    16861 cgaagcgtcc ggacgtaacg atctggtggt gaaaaccgtc gaaggcgacc gcaaagtctc
    16921 aggctcggcc tatcgcgaaa ccaaagatcg cggcttccac cacggcacct tgctactcaa
    16981 tgccgacctc agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa
    17041 aggcattact tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggctcac
    17101 catgagcagg tttgcgaggc cataaccgag gcctttttcg cccattatgg cgagcacgtg
    17161 gaagcggaaa tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt
    17221 gcccgtcaga gtagctgaga gtggaacttc ggtcaggctc cggcattctc gcatctgctg
    17281 gatgaacgct ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc
    17341 acccgcgccc aggtatttac cgacagcctc aaccccgcgc cgctggaagc cctcgccggg
    17401 cgactgcaag gctgcctgta ccgcgcggat atgctgcaac aagagtgcga agcgctgttg
    17461 gttgacttcc cggaccagga aaaagagcta cgggagttgt cgacgtggat agcgggggcg
    17521 gtaaggtaac tcgctgatta aaatatccaa ggaaggaaat atgatcgatc tggaaaacca
    17581 ggaacgggag attatcaatt taatgttcag ccagcgtatt tcctggctag ccgccgtgcg
    17641 tatcaggcat aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct cgattaattc
    17701 tctaaaacag atcgaaaaga cggaacggct tagctcaaac atcaaaagca aaatggcaga
    17761 aatttatggt tgtccaccag agttgctgat atgcccttcc tggatgacgg ctgaacataa
    17821 atgaatagat tcgtgaatag atacttgaat gttttttatt ttctatccat atgaaaaata
    17881 atagtaaatc tataaatcag ttgctattga tgaatagata ctaaaaagcc ggagcagtcc
    17941 cccactccgg cttaacacga caatcgcctt tcagcgaaac acaattactc tttatcgccc
    18001 agcagaacgg attccagtgc gattttgatc atgtcgttga aggtggtctg acgctcagcg
    18061 gcagtggtct gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt cagggctttc
    18121 gcgccaaact ctgcagccac gccgtagata ccagccgctt ccatttccac gccgaggatg
    18181 ccgtattttt ccatcacgtc gaacatttcg ccgtccggag agtagaacag gtcagcggag
    18241 aacaggttac ccacgcgagc atcaacgccc agtgctttag ctgcatctac tgcgttacgc
    18301 accatgtcaa agtcagcgat agcggcaaag tcatggtctt taaaacggat gcggttaact
    18361 ttggaatcgg tgcaggcccc cataccgata acgacgtcgc gcagttttac gtgcggcaga
    18421 actgcgccac aggaaccaac gcggataatt ttcttcacgc cgaaatcggt gatcagttct
    18481 ttggtgtaga tggagcagga cgggataccc ataccgtgac ccattacgga aattttgcgg
    18541 cctttgtaag taccagtgaa gcccagcata ccgcgaacgt tgttcacttc acgggcatct
    18601 tcaaggaaag tttcagcaat atacttcgca cgcagcgggt cgcctggcat caaaactacg
    18661 tcagcgaaat cgcccatttc tgcattaatg tgtggggtag ccattgtttt atcctttttg
    18721 tgacataaca aaggcggagt caacgctccg cccaaatcca tcagaacatg gctttgccat
    18781 attccatatc agaagtacca aaatattttg ccagagtctg gccgatatcc gcgaaggttt
    18841 cacggtgacc cagtgagccc ggttttactt tcgggccata taccagtacc ggaatgtgtt
    18901 cacgcgtgtg gtcagtaccg gtccaggtcg gatcgcaacc gtggtcagcg gtgaggatca
    18961 ggatgtcgtc atcgcgcagc agagacatca gctccggcag acggcggtcg aacagttcca
    19021 gacccgcagc ataaccggcg acgtcgcggc ggtggcccca ggaagagtcg aagtcaacga
    19081 agttggtgaa gacgatggtg ttatcacccg cttctttcat ctctttgatg gtggcgtcaa
    19141 acagcgcgtc caagccagtc gctttcactt ttttggtgat accgcagttg gcgtagatgt
    19201 ctgcaatttt accgacagaa accacctggc cgtgtttttc atcaaccagt ttctgcagca
    19261 cggtcggtgc tggcggctca acagccaagt cgtgacggtt accggtacgc tggaagttac
    19321 cagctttgtc accgataaac ggacgagcga taacacgacc gatattgtag ccgccgttgg
    19381 tcagctcttc acgggcgatt tcgcacagtt cgtagagttt atccagaccg aaagtttctt
    19441 catggcaggc aatctggaac acggagtcag cggaggtata gaaaatcggc ttgccggttt
    19501 tcatgtgctc ttcgcccagt tgatccagaa tgaccgtacc ggaagagtgg cagttaccga
    19561 ggtaacccgg cagattagcg cgttcgacca gtttatccag cagctcttgc gggaagctgt
    19621 tttcgtgatc ggagaaatat ccccactcaa acagaaccgg gacaccggcg atttcccagt
    19681 gaccagacgg ggtatcttta ccggatgaca tttcgtgcgc ccatgcgtac gcgccgataa
    19741 cttcagcgtt gccgtccatt cccgccggaa tgaaaccggt agaaccttcg tgtgctttcg
    19801 ccagccccag acgggtcaga tttggcagat tgagcggccc tttacgacca ttatcagctt
    19861 cgcctttggc acaagcttct gcgatatgac ccagggtgtc agccccgacg tcaccaaagc
    19921 gttctgcatc ttctgtagcg ccgatgccga atgagtccag caccataata aatgcacgtt
    19981 tcatatgttc tccgtactca gtgcttcaaa tttaaatgat cagatcagta taccgttatt
    20041 cgctgatacg gcgatagaca gttggtgtgc tttccggtgc gttatcggca agtttaattg
    20101 ccgctttcac cgctttcgcc gcttcctgcc agctgttttc gtctttcgcg aggataaccg
    20161 ccagcggacg ctgaccgtct acctggtcgc ccagacgcgc catatcagta aagccgacgc
    20221 tgtaatcgat ggtgtcagat gcctgacgac gaccgccgcc cattgcaact actgccatcc
    20281 ccagcgcgcg ggtatccatt tcactgacaa aaccttcggt atcagcatag actgctttcg
    20341 tcagcatcgc tgtcggcaga tacttcgcgt agttctcaac gaagtcggtc gggccttttt
    20401 gtgccgctac catacgacca aagacttctg ccgctttacc gttgtccagc accgcctgca
    20461 atttcgcgcg cgcttcggcg tcatctttcg ccagtttgcc ggagatcaac atctccacgc
    20521 acagcgccat cgtgacatca aacagacgcg ggttacgata ctcacccgtc aggaactgca
    20581 ccgcttcacg aacttcaacc gcgttacctg cactggaggc cagtacctga ttcatgtcgg
    20641 tgagcagcgc ggtggtgcgc acgccagcgc cgttagccac gccaacaatc gcttcggcaa
    20701 gggcttcaga gagttcgtag gtcggcataa acgcgccgct acccactttc acgtccatca
    20761 ccagcgcgtc cagaccttcc gccagtttct tcgccaggat cgaggcagtg atcagcggga
    20821 tggagtccac ggttgcggta atatcacggg tcgcgtagaa acgtttatca gccggagcca
    20881 gtgaactggt ctgaccgata atcgccacgc cgacgtcttt aataatttcg cggaaacggt
    20941 tgtcatccgg gaaaatgtcg aagccaggga tggattccag tttgtcgagc gtaccgccag
    21001 tatgaccgag gccgcgaccg gagatcatcg gaatatagcc gccgcaggct gcgaccatcg
    21061 gccccaacat cagcgaagtg acatcgccga cgccgccggt cgagtgttta tcaacaatcg
    21121 ggccattcag atgcaggctt ttccagtcga gaacggttcc tgaatctcgc atcgccatgg
    21181 tcagcgagac acgctcaggc attgtcatat cgtggaagaa aatggtcatc gcgagggcgg
    21241 caatctgccc ttcggaaata gtgttgtcgc gaataccgtt gataaagaaa cgaatttctt
    21301 catcgctcag cgcatgacca tcacgttttt tacgaataat ttcttgtgcg agaaacaagg
    21361 taacctcccg aggaaaagag tatgaggcgg cttttgccgg atgcgtctac gccttatccg
    21421 gcctacgggt agatacgtct cgtaggcctg ataagcgcag cgcatcaggc gtaaagcatc
    21481 ttacttagta gctgctggcg ctcttaccgt cgccgtggcc cagcgctttc agcaggcttg
    21541 ccagcaggct ggaagcacca aagcggtagt gacgcgcatc agcccagtca gcaccgaaca
    21601 gttcatctgc gatggcgaga tatttctgcg catcttccgc agtacgcacg ccgcccgccg
    21661 gtttgaaacc aacggttttt tctacgccca tatcacggat cacttccatc atgatgcgag
    21721 cgctttccgg cgtcgcgttc acagccactt taccggtaga ggttttgatg aagtccgcac
    21781 ccgctttgat ggagatttca gacgctttac ggatcagcgc ttcgtctttc agttcgccgg
    21841 tttcgatgat cactttcagc agtacattcg ctgccgcgca agcctcttta caggctttca
    21901 ccaggtcaaa accaacctgc tcgtcacccg ccatcagcgc gcggtacggg aacaccacgt
    21961 caacttcatc agcaccgtag gcgattgccg cacgggtttc tgccagcgcg atttcgatgt
    22021 cgtcgttacc gtgtgggaag ttggttaccg tagcgatacg gatttccggg gtgccctgct
    22081 ctttcagagt tttgcgagca atcgggataa aacgaggata gatacagata gcggcggtat
    22141 tgccgaccgg agttttggcc tgatgacaca gagcaattac tttctcgtcg gtgtcgtcgt
    22201 cattcagggt ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct ttcagatcag
    22261 tcatttcatt ctcctggctt gtcgccaata aaattcacct tgcgagtttg ttagtattct
    22321 aacatctact ccgcaacaca cttcgataca catcacaatt aaggaaatct acttacaagt
    22381 ttgcatcact gtaatgcgat ctggttcaaa taattcactt tcaaatgaat gcgtcagtgg
    22441 tggcaaacgc atcaggatca aagtgaacat cacgaaactt cttacaatgg cgcatcacct
    22501 tcggcataaa aggaaaagaa gatgcccact tctcatgaaa atgcactgca acaacgttgc
    22561 cagcaaattg tcaccagccc agtgcttagc ccggagcaga agcgccattt tctggcactg
    22621 gaagcagaaa acaatctgcc ttacccacag cttcctgccg aagcccgccg cgcgctggat
    22681 gaaggtgtaa tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg ctatgtctta
    22741 cccgattacg ctcgttttct ggcgaacggt tccgaatggc tggagctgga aggcgcgaaa
    22801 gatcttgatg acgcactctc tctgctgacc attctttacc accacgtacc gtcggtcaca
    22861 tcgatgccgg tctacctggg gcaactggat gcgttgttgc aaccgtatgt tagaattcta
    22921 acacaaaacg agatcgatgt tcgaataaaa cgtttctggc gttacctcga cagaaccctg
    22981 ccagatgcct ttatgcacgc caatatcggc ccgtctgatt cgcccattac ccgcgcgatc
    23041 ttacgtgcag acgcgcaggt ttcaccgaac ctgaccttta tctacgatcc tgaaatcacc
    23101 cctgatgacc tgctgctgga agtggcgaag aacatctgtg aatgtagcaa accgcacatc
    23161 gccaacggtc cggtgcatga taaaattttc acaaaagggg gctacgggat tgtgagctgt
    23221 tacaactcac tgccgctggc gggtggtggc agcacgctgg tacgccttaa cctgaaagcc
    23281 attgccgagc gcagcgaatc gctggatgac ttctttacgc gcactctacc gcactactgc
    23341 cagcagcaga tcgccatcat cgatgcgcgg tgtgaattcc tctatcaaca atcacacttc
    23401 tttgagaata gcttcctggt gaaagaaggg ctgattaacc ctgaacgttt tgtgccaatg
    23461 tttggcatgt atgggctggc ggaagcggtt aacttgctgt gtgagaaaga agggattgcc
    23521 gcgcgctacg gtaaagaagc cgccgcaaat gaagtgggtt atcgcatcag cgcgcaactg
    23581 gcggagtttg tcgccaatac ccccgtgaaa tatggctggc aaaaacgcgc catgttacac
    23641 gcacagtcgg ggatcagttc cgatatcggc accacgccgg gcgcgcgttt gccgtatggc
    23701 gatgagccag atccgatcac ccatctgcaa actgtcgccc cgcatcatgc ttattattat
    23761 tccggcatca gcgacattct gacgctcgac gaaaccatca aacgtaaccc gcaggcactg
    23821 gtacagcttt gcctcggtgc ctttaaagcc ggaatgcgtg aatttaccgc caatgtcagc
    23881 ggtaacgatc tggttcgcgt taccggttat atggtgcgtt tgtcggattt agaaaaatat
    23941 cgcgccgaag gttcacgcac caacaccacc tggctgggcg aagaagccgc acgcaacact
    24001 cgtattctgg aacgccagcc gcgcgtgata agccatgaac agcagatgcg ctttagtcag
    24061 taagattatc cccttctcct gcgttgacgg gccaggcagt cgtctggctc tgtttttgca
    24121 gggctgcaat ctgcgctgca aaaactgtca caatccgtgg acgatgggac gttgcaatga
    24181 ctgtggggag tgcgtgccac agtgtccgca tcaggcgttg cagattgttg acggcaaagt
    24241 ggtgtggaac gctgtggtct gcgagcaatg tgatacctgc ctgaagatgt gtccgcaaca
    24301 tgccacacca atggcgcaat ccatgagcgt ggacgaagtg cttagccatg tgcgcaaagc
    24361 ggtgctgttt attgaaggga taacggtaag cggcggcgaa gccacgaccc aactgccgtt
    24421 tgtagtggcg ctgtttactg ctatcaaaaa cgatccgcaa ctgcgccatc tcacctgtct
    24481 ggtggacagt aacggtatgt tgagcgaaac cggctgggaa aaattactcc cggtgtgtga
    24541 cggcgcaatg ctcgatctca aagcatgggg tagcgaatgt catcaacaat taaccggacg
    24601 cgataatcaa cagattaagc gcagcatcta tttgctggca gagcgcggca agctggcgga
    24661 actgcgtttg ctgatgatcc caggccaggt ggattatttg caacacatcg aagaactggc
    24721 ggcgtttatc aagggacttg gtgatgttcc ggttcgcctg aacgcgtttc atgcccacgg
    24781 cgtgtatggc gaggcgcaaa gctgggcgag cgccacgccg gaagacgttg agccgttggc
    24841 tgatgcatta aaggtgcgcg gggtgagccg gttgatattt ccggcgctct atttgtgaca
    24901 tgccggatac ggcctacggt tcggcacaga cttgtaggcc tgataagacg cgtcaagcgt
    24961 cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga acgccttatc cggcctacgg
    25021 ttcggcacaa acctgtaggc atgataagac gcgttaagcg tcgcatcagg cattatgcgc
    25081 cgactgccgg atgctgccta cgaagggcaa actacccacg aacgttaaac accgcatacg
    25141 tgttattaag caacacttcg gcaatctcat ccgcgggttc cgggcgtaac tcgcaaagca
    25201 cggcaaacac acgggcagcc tgttccgggc ggttaggctg cccctgaaaa ccgttgagcg
    25261 gcatatccgg cgcatcggtt tccagcaaca acgatgccag cggtaatttt gcgatgacat
    25321 cgcgggtttt actggcgcgt ggataggtga tagtaccgcc tacgccgatt ttgtagccca
    25381 gctgtacaaa ccgttcagcc tgttgctggc tgccagaaaa tccgtggaca acaccagcac
    25441 gcggcaaatc gtggcgttta agatgcatcg ccagtttgtc gtgcgtgcgc cgtgaatgca
    25501 ggatcaccgg cagatcgtag cgtttcgcca gtttcagttg ttcgtcgagt aaccactgct
    25561 gcctctcaaa ttgcggatcg tcgccgaaga gatccagacc gatctccccc accgccacca
    25621 ccttcgccgg acgcctttcc agcgcctgct gtagctgctc aagagacaca tcgctatgtt
    25681 tttccaacat accaggatgc aagcccaata cggcatacag cggttgataa ttttccgcta
    25741 acgcctgcac acgggcaaaa ttttccgcct ctgtcgccgg aacaatgatc ttgcctacgc
    25801 ccgcttgtgc cgcgcgttgc aggctggcct cttcatcgcc aataaaaggc gggaaatcaa
    25861 aatggcagtg agtgtcgata aaacggcaaa tcaagccaga tcctcattgt taaatgtcgg
    25921 gtcgttcgcc tgcggagcgt cgctcacttc ggcaaccagc gtatcgttgg cgactggcgc
    25981 aggcggaatg acaatcgatt ccggcgtcac cactggcacc agatggcggg taagcggcgc
    26041 tttttcagtt aatagcttgc caaccgtggc gaggaaataa cgaccgcata aacgcccaag
    26101 tttatagtct tcacgcagcg ccggaatccg actgccaagc gcgatactat gtaatggctt
    26161 cggcggataa atttcggata tccgcagctt gcccggtggt ttctcaataa attgctgaat
    26221 gtcacgatag ctggtttcat gatgctgcac cagattgacc agcggctgca ggctactgtc
    26281 acccagccag cgttccatgc gtttgaacca ctgcggcgtg tagtacattt gtgacggcac
    26341 agtgcggatg acgaccaacg ttttagcgcc ctgccttgcc gcctctttaa ccggaatcgc
    26401 atcactgatc ccgccatcca ggtagttaat gccttccagc gacactccgc tacgataaaa
    26461 gccaggtatc gcactggagg cgcgaatcac atccagccag ttttgtttgg ttggtaaaaa
    26521 gtaattcggc gcgtagtcat cctgacgaca ggcgcacata taaaacgatt tgccgctgtc
    26581 aaacaaccgc gcggcggtat ccatttgcaa cggcatctgg cttgcggtgg cctccaccag
    26641 ccagtcgaga tcgataagat ttcctccacg gacaaagcgc aatggatcga aaaattcgcg
    26701 ttttgtggta tagcgcatga tgactttgcg cgcgtaaccg ggctgattgc agataaacgc
    26761 cgagaggttc tgcgccccgg cagatgtgcc gagataaaga tcgaaaggat taaactgcgc
    26821 gcgcataaac tcatccagca cgccagccgt gaaaattcca cgctgtccgc cgccttcgca
    26881 caccagagct attcgtccag gctggaacgg ccttagcgac aacggcgcaa tattaccaag
    26941 cgttacaggt attcgctgcc ccacttctgc cttcctgttt ttgttgtgaa agaaagacaa
    27001 agtaacgcaa tttattattc gctaaaaccg caaaagccag tccgctggac tggctttgga
    27061 taaaattttc ttaatggtct gaaatctagg gtcgttttcg gcccatgaac aaactcacta
    27121 ggaacagaat aatcccgacg acaaagacaa ttttagctgc gcctgcagcg gtaccggcca
    27181 gaccaccaaa cccaagtgcg gcggcgatta acgcgataac cagaaatatg atgccccaac
    27241 gaaacataag cgtctccttt accatagtta atgtcaccgc ttgatatgag cgtgaaaatc
    27301 actcaatgcg atatttttag tgtggtgcac atcgcgcctc ccgacaaagt tcgggaggac
    27361 gaattactta gttttcagat catttttaac gcttttcaca ccatctaccg ctttggcgat
    27421 actttcagca cggtcacttt gtgcctgaga atctacggta ccggagagct gaaccacgcc
    27481 atcggtggtt tcaactttca ctttacgtga agggacgata tcgtccgcca gcagtttggc
    27541 tttgatttca ctggtggtgg cggtgtcacc cgcgtagccc ttcaccgagc cttctttagc
    27601 gtcgcgaacg tgcagtttgt cgctgacaga ggtcacgcct tcaacgcctt tcgccacttt
    27661 cactgcctct tcggcctggg cctgactttc aacgaaaccg ctcagggtca cgactttttg
    27721 atcggttttg acagagatat cggtgctctt gatgttgtca tgatccacca gcgccgcctt
    27781 cactttcgcg gtgatggcgc tgtcatccat gaaattaccg actttattca tagagctatc
    27841 gactttttgc cctgcgcttt cattggtagt ctgcgcgttg ttttccgcgt aggcagagcc
    27901 ggtcgcgacg gcagaggtca acattacagc cagcagagtt ttcgaaatct tcagtcttgt
    27961 catagtcatc gatttattcc tgtatgtttg ctcgtaattt gagcctagca acacgaggtt
    28021 gcattgctga atagggagag acttcaccct ctacagaagt caatggtcgc catcacaaaa
    28081 gcgatgggtg atgaataacg accattacag cctctgaatc agttattaat atcggtagaa
    28141 tgacaatcga cggctttaga tactaatatc tacgcattga acggtattta acgccatcag
    28201 aaatgtcatc actttgttaa atatagatca caattttgaa accgctcggg atatcagcga
    28261 aaacataagc aaaagtgaat gttttaagaa cattccgtaa gcggctaata aggaagggaa
    28321 attgacaggg cgcagcggct accgcgccct ggaggcaaga attaatgctc gcgggtctgg
    28381 tggaactgaa cgtccggata acgttcctgt gccaggcgca ggttgaccat gctggtagcg
    28441 atgtaagcga ggttatcgcc gccatcaagc gccagttggc tttcgttctt acgcttgaac
    28501 tcttcgaatt tcttcgcgtc tgcacattct acccagcggg cagtggcaac gttgactgac
    28561 tcatacactg cttcaacgtt gtattcgctc ttcaggcgcg ctaccaccac atcaaactgc
    28621 agcacaccaa ccgcaccaac gatcagatcg ttgttggaga ttggacggaa cacctgcacc
    28681 gcgccctctt cggaaagctg taccagccct ttgagcagct gtttttgctt cagcggatct
    28741 ttcaggcgga tacgacggaa cagttctggt gcgaagttcg gaataccggt gaacttcatc
    28801 atctcaccct gggtaaaggt gtcgccgatc tgaatggtgc cgtggttgtg caggccgagg
    28861 atatcgcccg gatacgcttc ttcaacgtgc gaacggtcac ccgccataaa ggtcagcgcg
    28921 tcggagatca ccacatcttt cgcagtgcgc acctggcgca gtttcatgcc tttttcatat
    28981 ttaccggaca ccacacgcat aaacgccacg cggtcgcggt gtttcggatc catgttggcc
    29041 tgaattttaa atacgaagcc ggtaaattta tcttcgctcg cttctacggt acgggtatcg
    29101 gtctgacgcg gcatcggcgc aggcgcccac tccaccaggc cattcagcat atgatcaacg
    29161 ccgaagttac ccagcgcagt accgaagaat accggagtga tttcgcccgc aaggaacagc
    29221 tctttgtcga actcgttaga cgcgccttta accagttcca gttcgtcacg cagctgctgt
    29281 gccagatctt caccaaccgc agcatcgaga tccgggttat tcagcccttt aacaatgcgg
    29341 acttcctgaa tggtgtgccc tttaccgctc tgatagagat aggtttcatc tttataaagg
    29401 tggtaaacgc ctttaaacag cttgccgcag ccaattggcc aagtgatcgg tgcgcagcca
    29461 attttcagct cgttctcaac ttcatcgagc agttccatcg ggtcgcggat atcacggtca
    29521 agtttgttca taaaggtgag gatcggcgtg tcgcgcagac gggtaacttc catcagctta
    29581 cgggtacgat cttcaacacc ttttgcggcg tcgataacca tcaggcagca gtccaccgcc
    29641 gtcagggtac gataggtatc ttccgagaag tcttcgtgcc ccggggtgtc gagcaggtta
    29701 accaggcaat cgtgatacgg aaactgcatc acagacgtag taatggagat cccacgctgc
    29761 ttttccatct ccatccagtc cgacttagcg tgctggttgg aaccacggcc ttttactgta
    29821 ccggcggtct gaatggcctg tccgaacagc agcaccttct cggtgatggt agttttaccg
    29881 gcgtccgggt gagaaataat ggcaaaagtg cggcgcttcg ccacctcttt caaataagga
    29941 gacaacgtca taattctttc ttcttgagta agcggcatcg cgccgcgctt gttgaaaacg
    30001 aaaaattgcg gctattttac ccatcaacgg gggggaggca atcagtgttt acacaggagc
    30061 tgctccagtt cgtgcaacga agaaacggtc caggtgggcg cgatgccttc tggttgctcg
    30121 cgattgtgcg cattcagcca gcaggtcgca agcccggcgt tgatgccacc gagaatatcg
    30181 gactcggcag tgtcgccaac catcagcacg cgtgaacggt caggattgcc cgcctgttcc
    30241 agcgcataat cgaaaatttt cttattcggt ttggcaacgc caacttcttc ggaaatcacc
    30301 agcaaatcga agtaatcacg caggcccgtc cgttccagac gcacttgctg caaggcgcta
    30361 aaaccgttgg tgatgatgcc gattttagcg ttgctacgaa tggcgttaag cagagaaacc
    30421 gcgcccggca acggcgtaca gatttccgcc atcgcattaa taaaggcatc gttaagcaag
    30481 cctgcttcaa ctttcagccg ttcggcccag ctctcgaaac gcccgtgctg aagctgtaat
    30541 gaagtgatcg cgccgttttg ataatccacc cacagtggct tgttaacggc ctgatagtcc
    30601 tgaaaatctt cagcggtaaa ggtgacgctg taatcaagaa acatccgctg caggccggtg
    30661 aatgagtcaa aggtaaacag cgtttcatcg gcatcaaaga aaatccagtc ccacttcatt
    30721 attccacctt gtcttacata ctgattggca acgccatgat gatggcgtct tcgcgaccgt
    30781 ccgtggtggg gtagtaattg cggcgaatcg tcgcctcgtt aaagcctaaa ctttcgtaca
    30841 gggcaatggc ggcagcattt gaggcacgga cttccagcca tagtgtcgcc acgccgcgtt
    30901 tttccagttc gtcgatcaga tgttccagca gcgccctacc cagcccctga cgctgatagt
    30961 cgggatcgac cgcaatattg aacaatgtag cttcatccag caccacttgc gtaatcgcaa
    31021 acgccgccat tttgccgttt tgcgttaact gaaagttgag ataacgctcg ccctggttgc
    31081 tggcaaaggt tttttcactc cacggaaagg cgtgggcgcg ttgttcaatg tggtaagccg
    31141 ctggtaaatc agtcgtttcg agggaagaaa tcgtgttcat atgtgcaaat ttgttgccat
    31201 aacgcggcgc gtgccgttgg gtttgcccgt aattcggtga gcgccggtga tgccacctga
    31261 gcgccttcca atgatagcgg ttcgtcagta cccaaccgcc agctgttgca gcgactgcct
    31321 tgcggcagca tcgcgatttt ttctggcgtc agttgcagca cttgatcggg gctgacggtt
    31381 aatgcgcgca gaacatcgct cactaaagga tcagtcaggg cgggaagatc gtttgccacc
    31441 atcaccagac ggacgtgtgc cgggctcgca atggcaatct ccccctgcaa cgcgccaggg
    31501 cgacgcagcg accactgggt aatgcccagt tgctgtaact gccagtctcg tcgggatgtc
    31561 atagcgaatc gctcctgttg tcaggggcgc aaatatagca aattcgtcga taccgcgcca
    31621 acatatggct ataatcgccg ccagtatcaa ttgaggagca ttccatgtct gcatttaccc
    31681 cggcaagtga agtcttgctg cgtcacagtg atgatttcga acaaagccgt attctgtttg
    31741 ccggagactt acaggatgac ctgcccgcgc gtttagatac cgcggccagc cgtgctcata
    31801 cccagcaatt ccaccattgg caggtgttaa gctgccagat gggggataac gcccgtttta
    31861 gtctggtcgc cacggcgaat gatgtcgcag attgcgatac gctgatttac tactggccga
    31921 agaacaaacc ggaagcccag ttccagttga tgaatttact ttctctgctg ccagtgggga
    31981 cagatatttt tgtcgttggc gagaaccgca gcggcgtgcg cagcgctgag cagatgctgg
    32041 cagattatgc gccgttgaat aaagtcgaca gcgctcgtcg ctgtggcctc tattttggtc
    32101 gtctggaaaa acagccagta tttgatgcgg ataaattctg gggcgaatac agcgtcgatg
    32161 gcctgacggt caaaacgctg cctggcgtgt ttagccgcga cggtctggat gtcggtagcc
    32221 agttgctgct ctcgacgtta actccgcaca cgaaaggtaa agtgctggat gtcggctgtg
    32281 gcgcgggtgt actttcagtt gcctttgcgc gccattcgcc gaaaattcgt ctcaccttgt
    32341 gcgatgtctc tgccccggcg gtagaagcca gccgcgcaac acttgcagcc aacggtgttg
    32401 aaggtgaagt ctttgccagc aacgtctttt ccgaggtgaa aggttgtttt gatatgatca
    32461 tctccaaccc gccgttccac gatgggatgc aaaccagcct ggatgcggcg caaacgctga
    32521 ttcgtggcgc ggtgcgtcat cttaatagcg gcggcgagct gcgaattgta gcgaacgcct
    32581 tcctgcctta cccggacgtg ctggatgaga catttggctt ccatgaagtt atcgcgcaaa
    32641 ccgggcgctt caaggtatat cgcgccatta tgacccgcca ggcgaagaaa ggttgagtat
    32701 ctgatatctg tcgccgcata ccgtgtcgga tgcggcgtga acgccttatc cgacctacaa
    32761 aacctgcaca ttagcccttc gtaggccaga taagacgcgc cagcgtcgca tctggcatct
    32821 gcccccgcat gcctaccaaa cctgcacgct agtttcctga tggacatttt tccagcaatt
    32881 acacatcagg cgataattaa ctattgacga aaagctgaaa accactagaa tgcgcctccg
    32941 tggtagcaat tctttttaag aattgatggt atgcgaaggt ggcggaattg gtagacgcgc
    33001 tagcttcagg tgttagtgtc cttacggacg tgggggttca agtccccccc ctcgcaccaa
    33061 aaatcacgtt gatattgctc gcactgggcg aaggtggcgg aattggtaga cgcgctagct
    33121 tcaggtgtta gtgttcttac ggacgtgggg gttcaagtcc cccccctcgc accaaacga
//
LOCUS       DAZSNO010000031        31965 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZSNO010000031 DAZSNO010000000
VERSION     DAZSNO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31965
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.031"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(80..970)
                     /gene="rarD"
                     /locus_tag="ACHWN2_001410"
     CDS             complement(80..970)
                     /gene="rarD"
                     /locus_tag="ACHWN2_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR8931004.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1022..1489)
                     /gene="yigI"
                     /locus_tag="ACHWN2_001411"
     CDS             complement(1022..1489)
                     /gene="yigI"
                     /locus_tag="ACHWN2_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR8931005.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1654..2523
                     /gene="pldA"
                     /locus_tag="ACHWN2_001412"
     CDS             1654..2523
                     /gene="pldA"
                     /locus_tag="ACHWN2_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR8931006.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2656..4485
                     /gene="recQ"
                     /locus_tag="ACHWN2_001413"
     CDS             2656..4485
                     /gene="recQ"
                     /locus_tag="ACHWN2_001413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR8931007.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4549..5169
                     /gene="rhtC"
                     /locus_tag="ACHWN2_001414"
     CDS             4549..5169
                     /gene="rhtC"
                     /locus_tag="ACHWN2_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR8931008.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5231..5851)
                     /gene="rhtB"
                     /locus_tag="ACHWN2_001415"
     CDS             complement(5231..5851)
                     /gene="rhtB"
                     /locus_tag="ACHWN2_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR8931009.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5962..6984
                     /gene="pldB"
                     /locus_tag="ACHWN2_001416"
     CDS             5962..6984
                     /gene="pldB"
                     /locus_tag="ACHWN2_001416"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR8931010.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6992..7792
                     /gene="yigL"
                     /locus_tag="ACHWN2_001417"
     CDS             6992..7792
                     /gene="yigL"
                     /locus_tag="ACHWN2_001417"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR8931011.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7868..8767
                     /gene="bioP"
                     /locus_tag="ACHWN2_001418"
     CDS             7868..8767
                     /gene="bioP"
                     /locus_tag="ACHWN2_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR8931012.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8655..9608)
                     /gene="metR"
                     /locus_tag="ACHWN2_001419"
     CDS             complement(8655..9608)
                     /gene="metR"
                     /locus_tag="ACHWN2_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR8931013.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9844..12105
                     /gene="metE"
                     /locus_tag="ACHWN2_001420"
     CDS             9844..12105
                     /gene="metE"
                     /locus_tag="ACHWN2_001420"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR8931014.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12144..12959)
                     /locus_tag="ACHWN2_001421"
     CDS             complement(12144..12959)
                     /locus_tag="ACHWN2_001421"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR8931015.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13221..13982
                     /gene="udp"
                     /locus_tag="ACHWN2_001422"
     CDS             13221..13982
                     /gene="udp"
                     /locus_tag="ACHWN2_001422"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR8931016.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14123..15550
                     /gene="rmuC"
                     /locus_tag="ACHWN2_001423"
     CDS             14123..15550
                     /gene="rmuC"
                     /locus_tag="ACHWN2_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR8931017.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15645..16400
                     /gene="ubiE"
                     /locus_tag="ACHWN2_001424"
     CDS             15645..16400
                     /gene="ubiE"
                     /locus_tag="ACHWN2_001424"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR8931018.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16414..17019
                     /gene="ubiJ"
                     /locus_tag="ACHWN2_001425"
     CDS             16414..17019
                     /gene="ubiJ"
                     /locus_tag="ACHWN2_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR8931019.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17016..18656
                     /gene="ubiB"
                     /locus_tag="ACHWN2_001426"
     CDS             17016..18656
                     /gene="ubiB"
                     /locus_tag="ACHWN2_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR8931020.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18735..19004
                     /gene="tatA"
                     /locus_tag="ACHWN2_001427"
     CDS             18735..19004
                     /gene="tatA"
                     /locus_tag="ACHWN2_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR8931021.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19008..19523
                     /gene="tatB"
                     /locus_tag="ACHWN2_001428"
     CDS             19008..19523
                     /gene="tatB"
                     /locus_tag="ACHWN2_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR8931022.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19526..20302
                     /gene="tatC"
                     /locus_tag="ACHWN2_001429"
     CDS             19526..20302
                     /gene="tatC"
                     /locus_tag="ACHWN2_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR8931023.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20344..21126
                     /gene="tatD"
                     /locus_tag="ACHWN2_001430"
     CDS             20344..21126
                     /gene="tatD"
                     /locus_tag="ACHWN2_001430"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR8931024.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21123..21611)
                     /gene="rfaH"
                     /locus_tag="ACHWN2_001431"
     CDS             complement(21123..21611)
                     /gene="rfaH"
                     /locus_tag="ACHWN2_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR8931025.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21778..23271
                     /gene="ubiD"
                     /locus_tag="ACHWN2_001432"
     CDS             21778..23271
                     /gene="ubiD"
                     /locus_tag="ACHWN2_001432"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR8931026.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23317..24018
                     /gene="fre"
                     /locus_tag="ACHWN2_001433"
     CDS             23317..24018
                     /gene="fre"
                     /locus_tag="ACHWN2_001433"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR8931027.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24210..25373)
                     /gene="fadA"
                     /locus_tag="ACHWN2_001434"
     CDS             complement(24210..25373)
                     /gene="fadA"
                     /locus_tag="ACHWN2_001434"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR8931028.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25383..27572)
                     /gene="fadB"
                     /locus_tag="ACHWN2_001435"
     CDS             complement(25383..27572)
                     /gene="fadB"
                     /locus_tag="ACHWN2_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR8931029.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27762..29093
                     /gene="pepQ"
                     /locus_tag="ACHWN2_001436"
     CDS             27762..29093
                     /gene="pepQ"
                     /locus_tag="ACHWN2_001436"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR8931030.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29093..29707
                     /locus_tag="ACHWN2_001437"
     CDS             29093..29707
                     /locus_tag="ACHWN2_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR8931031.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29746..31197
                     /gene="trkH"
                     /locus_tag="ACHWN2_001438"
     CDS             29746..31197
                     /gene="trkH"
                     /locus_tag="ACHWN2_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR8931032.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31209..31754
                     /gene="hemG"
                     /locus_tag="ACHWN2_001439"
     CDS             31209..31754
                     /gene="hemG"
                     /locus_tag="ACHWN2_001439"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR8931033.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7424 a         8139 c         8741 g         7661 t
ORIGIN      
        1 gtgtgagtct cctgtcagca tggcaatatg cccactctca tacaagagtc ggcatatttt
       61 ttcagaatat atttattttt tatttggacg ttctacgctg cgtataaatc gcgtccatca
      121 caaaaattgc cagcgccacc caaataaagg tgaaagtcac catcttatcg gcacccggtt
      181 tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg tactgaaaaa
      241 agcctaacgt tgagagacgc aggcgcgtgg cagcggcagt aaaacacaac agcggtacgg
      301 tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt tgccccatat
      361 ggctggttga actgtcggca atagcaaaca ggtaaatcgc cgccacgggc agcagccaca
      421 tggtttcaat taacatgccg gtttgtgctt ctaccgcaat cttcttgcgc accaggccgt
      481 agaaggcaaa actaaatgcc agccccagcg cgataatagg tagcgaacca aaagtccaca
      541 gctggaccaa cacgccacat atcgccagaa tcaccgccag ccattgcatc cggcggaatc
      601 gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata aagtaaccaa
      661 ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc cagttgccac
      721 caatcagtac ggcagagact gccagcataa aaattttctg tggcgtctga atcagcgttt
      781 ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa aacgaccaga
      841 tcacgcgatg cgtcaggatc tcatcggcgg gcacgtagta aatcagcttg aagtatgctg
      901 gcgcaatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc cgcgtttgtt
      961 ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg cttttatgtc
     1021 ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct tcattgtgta
     1081 attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg gtagcagtaa
     1141 aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc cccatccgcg
     1201 atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag gtacttccca
     1261 cgcacaccag accggcggcg acatccagcg ccgacgcaat gactccgccg tgcaaaatgc
     1321 tttgcgccca gttacccacc atcatcggct gatttttaaa ggccagctgt gcgaactcct
     1381 tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga taaacaaaca
     1441 tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata tcatccttac
     1501 acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt ctactttagg
     1561 aagggataac taacggcaat ggagttaagt atgtaaaata gcccgcagaa aaacattcac
     1621 cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg ctggttgttg
     1681 ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa agaggtgcat
     1741 gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca tgacaatccg
     1801 ttcacgctct atccttatga caccaactac ctcatctaca cccaaaccag cgatctgaat
     1861 aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga agtaaagttt
     1921 cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccaaactc ggtgttgggt
     1981 gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc ttcaccgttt
     2041 cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta ccgttttgca
     2101 ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg gcgttcagac
     2161 ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa cggtaactgg
     2221 ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc ggatatcacc
     2281 aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc ggtgctcagt
     2341 gcgaaaggac agtacaactg gaacaccggc tacggcggcg cggagttagg cttaagttac
     2401 ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg cgaatcgctc
     2461 atcgactata acttcaacca gacccgtgtc ggtgtagggg ttatgctaaa cgacttgttt
     2521 tgatgaacgg ttgagtggtt ggcaaatctg gaatccagca tccaggatta ccctctcaga
     2581 gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt tttatttcag
     2641 gcaatcgggg tgaatgtggc gcaggcggaa gtgttgaatc tggagtccgg agctaaacag
     2701 gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga aattatcgac
     2761 actgtgcttt ccggtcgcga ttgcctggtt gttatgccca ctggtggcgg aaaatccctt
     2821 tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc accgctgatt
     2881 tcgttgatga aagatcaggt ggatcaacta caggccaacg gcgtggcggc ggcgtgcctt
     2941 aactcgacgc aaacccgcga acagcaactt gaagtgatga caggctgccg caccgggcaa
     3001 attcgcttac tgtatatcgc cccggaacgc ctgatgctgg ataactttct tgagcatctg
     3061 gcgcactgga atccggtgtt attagccgtc gatgaagcgc actgtatctc ccaatggggt
     3121 cacgatttcc gcccggaata tgccgcgctc ggtcagttgc gccagcggtt cccgacgctg
     3181 ccgtttatgg cgctgaccgc cacagccgac gacaccacgc gccaggatat cgtgcgcctg
     3241 ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa tattcgctac
     3301 atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca ggaacagcgc
     3361 ggtaagtctg gcattatcta ctgcaacagc cgggcgaaag tagaagacac cgctgcgcgc
     3421 ctgcaaagca ggggtattag cgcggcggcc tatcatgccg ggctggaaaa taatgtccgc
     3481 gccgacgtgc aggagaaatt ccagcgcgat gatctgcaaa ttgtggtggc gacggtggcg
     3541 ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga tattccgcgc
     3601 aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct gcctgcggaa
     3661 gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct ggaagagaag
     3721 ccgcaggggc agttgcagga tatcgagcgc cacaaactca atgcgatggg cgcgtttgcc
     3781 gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg gcgtcaggag
     3841 ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg ttcaaccgat
     3901 gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat gggttatgtg
     3961 gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca tgacaaactg
     4021 aaagtctatg gtatgggccg tgataaaagc catgaacatt gggtgagcgt gatccgccag
     4081 ctgattcatc tcggcctggt gacgcaaaat attgcccagc attctgccct acaactgaca
     4141 gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt gccgcgtatc
     4201 gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga tcgcaaactg
     4261 ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt cccgccgtac
     4321 gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat caccgccagc
     4381 gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg taaaccgttt
     4441 atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc agcataaaaa
     4501 agtgccagta tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat gttgatgtta
     4561 tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc cgatttcttt
     4621 tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg cgtgctgggc
     4681 attacctgcg gcgtaatggt ttgggctggg attgcgttgc ttggcctgca tttgattatc
     4741 gaaaaaatgg cctggctgca tacgctgatt atggtaggcg gtggcctgta tctctgctgg
     4801 atgggttacc agatgctacg cggtgcactg aaaaaagagg ctgtatctgc gcctgcacca
     4861 caggttgagc tggcgaaaag cgggcgcagt ttcctgaaag gcttactgac caatctcgcc
     4921 aatccgaaag cgattatcta ttttggttcg gtattctcat tgtttgtcgg cgataacgtt
     4981 ggcactaccg agcgctgggg cattttcgcg ttgattattg tcgaaacact ggcgtggttt
     5041 accgtcgttg ccagtctgtt tgccctgccg caaatgcgcc gtggttatca acgtctggcg
     5101 aagtggattg atggttttgc cggggcgtta tttgccggat ttggcattca tttgattatt
     5161 tcgcggtgat gccagacgcg tcttcagagt aagtcggata aggcgtttac gccgcatccg
     5221 acattatttt tcacgcatgc ctcgccgatg ctaacagcgc tcccaccagc ataaacaacg
     5281 agccgaaaat cttattcagc gccttcatct gctttggtcc tttaatccat agagcaatcc
     5341 tttgagcaag ggtggcgtaa ccgatcatca caataatatc gaccacaata gtggtgacgc
     5401 cgagcacgat atactgcatc agttgcggct gttgcggcat gatgaattgc ggaaatagcg
     5461 ccgccagaaa cacaatactt ttgggattgg tgagattcac aaaaactgcg cgctggaaca
     5521 aatgtcgacg cgattgagta gaggccagcg atttaagatc aattgcgcca gcggcgcgcc
     5581 actgctggat tcccagccaa atcaagtaag ccgcgcctgc ccactttaac acttcaaacg
     5641 caatcactga gcgggaaaat agcgtcccca accccacgcc aaccagcaca atatgaatcg
     5701 ccagtccggt ctgaagccca gcaatagacg ccaccgcgcc gcgataaccg tggttgagcg
     5761 aggtggtcat agtgttgatt gcaccagagc ctggcgacag gcttaaaatg atcgatgtca
     5821 gcaggtaggc aaaccaccat tctaaggtca tgatgaactc ccggtgtgtc tatttttgtg
     5881 ccacaatacg ctactgtcgc agcgttgtgt caggcacgct taaaaaaacg attttacgtg
     5941 gtttaagagg cagattaccc gatgtttcag cagcaaaaag actgggaaac aagagaaaac
     6001 gcgtttgctg cttttaccat gggaccgctg actgatttct ggcgtcagcg tgatgaagca
     6061 gagtttactg gtgtggatga cattccggtg cgctttgtcc gttttcgcgc acagcaccat
     6121 gaccgggtgg tagtcatctg cccggggcgt attgagagct acgtaaaata tgcggaactg
     6181 gcctacgacc tgttccattt ggggtttgat gtcttaatca tcgaccatcg cgggcaggga
     6241 cgttccggtc gcctgttagc cgatccgcat ctcgggcatg ttaatcgctt taatgattat
     6301 gttgatgatc tggcggcatt ctggcagcag gaggttcagc ccggtccgtg gcgtaaacgc
     6361 tatatactgg cacattcgat gggcggtgcg atctccacat tatttctgca acgccatcca
     6421 ggtgtatgtg acgccattgc gctaactgcg ccaatgtttg ggatcgtgat tcgtatgccg
     6481 tcatttatgg cacggcagat cctcaactgg gccgaagcgc atccacgttt ccgtgatggc
     6541 tatgcaatag gcaccgggcg ctggcgcgcg ttgccgtttg ctatcaacgt actgacccac
     6601 agcagacagc gatatcgacg taacttacgc ttctatgctg atgacccaac gattcgcgtc
     6661 ggtgggccga cctaccattg ggtacgcgaa agtattctgg ctggcgaaca ggtgttagcc
     6721 ggtgcgggtg atgacgccac gcaaacgctt ctcttacaag ctgaagagga acgcgtggtg
     6781 gataaccgca tgcacgaccg tttttgtgaa ctccgcaccg ccgcgggcca tcctgtcgaa
     6841 ggaggacggc cgttggtaat aaaaggtgct taccatgaga tcctttttga aaaggacgca
     6901 atgcgctcag tcgcgctcca cgccatcgtt gattttttca acaggcataa ctcacccagc
     6961 ggaaaccgct ctacagaggt ttaaatttct tatgtaccag gttgttgcgt ctgatttaga
     7021 tggcacgtta ctttctcccg accatacgtt atccccttac gccaaagaaa ctctgaagct
     7081 gctcaccgcg cgcggcatca actttgtgtt tgcgaccggt cgtcaccacg ttgatgtggg
     7141 gcaaattcgc gataatctgg agattaagtc ttacatgatt acctccaatg gtgcgcgcgt
     7201 tcacgatctg gatggcaatc tgatttttgc tcataacctg gatcgcgaca ttgccagcga
     7261 tctgtttggc gtagtcaacg acaatccgga catcattact aacgtttatc gcgacgacga
     7321 atggtttatg aatcgccatc gcccggaaga gatgcgcttt tttaaagaag cggtgttcaa
     7381 atatgcgctg tatgagccgg gattactgga gccggaaggc atcagcaaag tgtttttcac
     7441 ctgcgattcc catgaacaac tgctgccgct ggagcaggcg attaacgctc gctggggcga
     7501 tcgcgtcaac gtcagtttct ctaccttaac ctgtctggaa gtgatggcgg gcggcgtgtc
     7561 gaaaggccat gcgctggaag cagtggcgaa gaaactgggc tacagcctga aggattgtat
     7621 tgcgtttggt gacgggatga acgacgccga aatgctgtcg atggcgggga aaggctgcat
     7681 tatgggcagt gcgcaccagc gtctgaaaga tctgcatccg gagctggaag ttattggtac
     7741 taatgccgac gacgcggtgc cgcattatct gcgtaaactc tatttatcgt aatcgttctt
     7801 tatttggtca gttgtcaacc tgatacttcg ctacaatgga tactcgttaa tcaaagagtt
     7861 ttccattgtg gcgttactta tcatcaccac gattctgtgg gccttctcct ttagctttta
     7921 tggcgagtac cttgcggggc acgtcgatag ctattttgcg gtgctggtgc gcgttggctt
     7981 ggcggcactc gtttttctgc cgtttctgcg tacccgtagc aatagcctga aaacggtcgg
     8041 cctgtatatg ctggtgggcg cgatgcagct cggcgtgatg tatatgctga gtttccgcgc
     8101 ttatctctac ctgacggtct ccgaattgtt gctgttcacc gtactgacgc cgctctacat
     8161 cacgctgatt tatgacatca tgagtaagcg ccgtctgcgc tggggctatg cttttagcgc
     8221 cttgctggcg gtgattggtg ccgggattat tcgctatgat caggtcaccg accatttctg
     8281 gactggcttg ttgctggtgc aactctccaa tatcactttt gccatcggca tggtgggtta
     8341 caaacgcctg atggaaactc gcccgatgcc gcagtataac gcctttgcgt ggttctatct
     8401 tggcgcgttc ctggtggcgg taattgcatg gttcttgctg ggaaatgcgc agaaaatgcc
     8461 gcaaaccacg ttgcaatggg gcattctggt gtttcttggc gtggtggctt ccgggattgg
     8521 ctactttatg tggaactacg gcgcgacgca ggtggacgcc ggaacgctgg gcattatgaa
     8581 taatatgcac gttccggcag ggctgctggt aaacctggct atctggcacc aacagccgca
     8641 ctggccaacg tttattacag gcgcgctggt gatcctggcc tcactgtggg tgcatcgtaa
     8701 gtgggtcgct ccgcgctctt cacaaacggc agatgatcgc aggcgtgatt gcgcgctgag
     8761 cgaataaacg cttccgtaac tggctgacgc tgctcgccat cgcgcacggc ggcgtacagt
     8821 cggctccaca agccttcgcc cagggttttg gtcaccacca gaccctggcg ctcaaaactc
     8881 tctactaccc aatgcggtag cgcggcaata cccatccgcg cggcaaccat ttgaatcaac
     8941 aataaggtgt tatcgacgct tttcagtgac gggctgacgc ctgccggctg aagaaaatgc
     9001 cgccagacat ccagtcgact acgctgcacc ggataaatta atagcgtctc gctggcgaga
     9061 tcttccggtg taatacgcgt ttttgccgcc agtggatggt caggtgctaa caccaggcgt
     9121 acttcataat cgaacatcgg cgaataatgc aggccactgc gcggcaggat atcggaggtc
     9181 atcaccagat ccagctcgcc ctgttgcaac gcgggctgcg ggtcaaacgt cacgcccgat
     9241 ttaaaatcca tctctacctg cggccagttc ttatggaaat ttcctaaagc gggtgtcagc
     9301 cactgaatac agctatggca ctcaatggca atgcgcagac gcgtctgctg cggttcattg
     9361 caggcttgca gtgcctggct gatttgcggc agcacctggt ttgccagttg caacaggatt
     9421 tctccctgcg gtgtaaagcg tagcggctgg ctcttacgca caaatagccg gaagccaagg
     9481 cgttgttcca gatcgctaaa ctggtgagac agggcggatt gcgtctgatg caacgttgcc
     9541 gcagcggctg cgagcgagcc gcagttccgc aacgcttgta gcgttttcag gtgttttact
     9601 tcgatcatga aagtccttca cttcggcatg aataatttgc gcttgaggaa tatacagtaa
     9661 ccgccaatta tggatgtgta aacatctgga cggctaaaat cattcgtctt ttaaatttat
     9721 ggtgcgttgg ctgcgtttct ccaccccggt cacttacttc agtaagctcc cggcgatgaa
     9781 taaacttgcc gccttcccta aattcaaaat ccataggatt tacatataat tagaggaaga
     9841 aaaatgacaa tattgaatca caccctcggt ttccctcgcg ttggcctgcg tcgcgagctg
     9901 aaaaaagcgc aagaaagtta ttgggcgggg aactccacgc gtgaagaact gctgacagta
     9961 gggcgtgaac tgcgtgctcg tcactgggat caacaaaagc aagcgggtat cgacctgctg
    10021 ccggtgggcg attttgcctg gtacgatcat gtactgacca ccagtttgct gctgggtaat
    10081 gttccgccac gtcatcagaa caaagacggt tcggtagata tcgacaccct gttccgtatt
    10141 ggtcgtggac gtgcgccgac tggcgaacct gcggcggcag cggaaatgac caaatggttt
    10201 aacaccaact atcactacat ggtgccggag ttcgttaaag gccaacagtt caaactgact
    10261 tggacgcagc tgctggagga agtggacgag gcgctggcgc tgagccacaa cgttaagcct
    10321 gtgctgctgg ggccggttac ttacctgtgg ctggggaaag tgaaaggtga acagtttgac
    10381 cgccttagcc tgctgaacga cattctgccg gtttatcagc aagtgctggc agaactggcg
    10441 aaacgcggca tcgagtgggt acagattgat gaacccgcgc tggtactgga actaccacag
    10501 gcgtggctgg acgcatacaa acccgcttac gacgcgctcc agggacaggt gaaactgctg
    10561 ctgaccacct attttgaagg cgtaacgcca aatctcgaca cgattactgc gctgcctgtt
    10621 caggggctgc atgtggacct tgtacatggt aaagataacg ttgttgaact gcacaagcgc
    10681 ctgccttctg actggctgct gtctgcgggt ctgatcaatg gtcgtaacgt ctggcgcgcc
    10741 gatcttactg agaaatatgc gcaaattaag gacattgtcg gcaaacgtga tttgtgggtg
    10801 gcatcttcct gctcgttgct gcacagcccc atcgacctga gcgtggaaac gcgtcttgat
    10861 gcagaagtga aaagctggtt tgccttcgcc ctacaaaaat gccatgaact ggcactgctg
    10921 cgcgatgcgc tgaacagtgg tgacacggca gctctggcag agtggagcgc cccaattcag
    10981 gcgcgtcgtc actctactcg tgtacataat ccggcggtag aaaagcgtct ggcggcgatc
    11041 accgcccagg acagccagcg tgcgaatgtc tatgaagtgc gtgctgaagc ccagcgtgcg
    11101 cgttttaaac tgccagcgtg gccgaccacc acgattggtt ccttcccgca aaccacggaa
    11161 attcgtaccc tgcgtctgga tttcaaaaag ggcaatctcg acgccaacaa ctaccgcacg
    11221 ggcattgcgg aacatatcaa gcaggccatt gttgagcagg aacgtttggg actggatgtg
    11281 ctggtacatg gcgaggccga gcgtaatgac atggtggaat actttggcga gcacctcgac
    11341 ggatttgtct ttacgcaaaa cggttgggta cagagctacg gttcccgctg cgtgaagcca
    11401 ccgattgtca ttggtgacat tagccgcccg gcaccgatta ccgtggagtg ggcgaagtat
    11461 gcgcaatcgc tgaccgacaa accggtgaaa gggatgctga cggggccggt gaccatactc
    11521 tgctggtcgt tcccgcgtga agatgtcagc cgtgaaacca tcgccaaaca gattgcgctg
    11581 gcgctgcgtg atgaagtggc cgatctggaa gccgctggaa ttggcatcat ccagattgac
    11641 gaaccggcgc tgcgcgaagg tttaccgctg cgtcgtagcg actgggatgc gtatctccag
    11701 tggggcgtag aggccttccg tatcaacgcc gccgtggcga aagatgacac acaaatccac
    11761 actcacatgt gttattgcga gttcaacgac atcatggatt cgattgcggc gctggacgca
    11821 gacgtcatca ccatcgaaac ctcgcgttcc gacatggagt tgctggagtc gtttgaagag
    11881 tttgattatc caaatgaaat cggtcctggc gtctatgaca ttcactcgcc aaacgtaccg
    11941 agcgtggaat ggattgaagc cttgctgaag aaagcggcaa aacgcattcc ggcagagcgc
    12001 ctgtgggtca acccggactg tggcctgaaa acgcgcggct ggccagaaac ccgcgcggca
    12061 ctggcgaaca tggtgcaggc ggcgcagaat ttgcgtcggg ggtaaatcca aaccgggtgg
    12121 taataccacc cggtcttttc tcattacagc gacttcttcc caccatactg cttaaaccat
    12181 tccagcatac gctgccagcc atctttcgca gattcggcat gatagctcgg gcgataatca
    12241 gcgttgaatg catgcccggc atccgggtac acgataatct ctgctttcgc attagcagcc
    12301 cgcagcgcct ggcgcatcgt ttcaacgctc tcctgcggaa tgctgttatc ctgaccacca
    12361 tataagccga gaaccggcgc gttaagatcg gttgcgatat caacaggttg tttcggtgaa
    12421 ttcagcgact tgtcgcccgt cagtttgccg taccacgcca ctgcggcttt tagctgtgga
    12481 ttatgcgcgg catacagcca ggtgatacgt ccaccccagc agaatccggt gatcattaaa
    12541 cgatgaacat cgccgccgtt gcgggaagcc caactggcga catgatcgag atcggccagc
    12601 acctgcgagt caggcacttt tgctaccaga ccgctaagca acgtggggat atcggcaaaa
    12661 tcattcggat cgccttcgcg gaagtaaagt tcaggtgcga tagccagata cccctccagc
    12721 gccagacggc gacaaatgtc gcggatatgt tcatgcacgc caaaaatttc ctgcactaca
    12781 atgaccactg gcagtgggcc atcgctttgc tttggtctgg catggtaagc aggcatgtta
    12841 tccccttgtg aagggataga ggtgaagccc gccacaatcg cgtcgtccgg ggtctgaacg
    12901 atggtcgaag cgagaggcga tgcagcaggt gcaaatccag attgttgtgt tgttgccatg
    12961 gtattctccg tacccttata aaaatgttgc gcaatgttaa ctatagtcag catgcaacaa
    13021 atcacattgc ctgaatcggc tcatctttta tgcagtcctg cagaatgaag agtgatttat
    13081 gtgatatgaa tcacttttgg tgggtaaatt tatgcaacgc atttgcgtca tggtgatgag
    13141 tatcacgaaa aaatgttaaa cccttcggta aagtgtcttt ttgcttcttc tgactaaacc
    13201 gattcacaga ggagttgtat atgtccaagt ctgatgtttt tcatctcggc ctcactaaaa
    13261 acgatttaca aggggctacg cttgccatcg tccctggcga cccggatcgt gtggaaaaga
    13321 tcgccgcgct gatggataag ccggttaagc tggcatctca ccgcgaattc actacctggc
    13381 gtgcagagct ggatggtaaa cctgttatcg tctgctctac cggtatcggc ggcccgtcta
    13441 cctctattgc tgttgaagag ctggcacagc tgggcattcg caccttcctg cgtatcggta
    13501 caacgggcgc tattcagccg catattaatg tgggtgatgt cctggttacc acggcgtctg
    13561 tccgtctgga tggcgcgagc ctgcacttcg caccgctgga attcccggct gtcgctgatt
    13621 tcgaatgtac gactgcgctg gttgaagctg cgaaatctat tggcgcgaca actcacgttg
    13681 gcgtgacagt ttcttctgat accttctacc caggtcagga acgttacgat acttactctg
    13741 gtcgcgtagt tcgtcacttt aaaggttcta tggaagagtg gcaggcgatg ggcgtaatga
    13801 actatgaaat ggaatctgca actctgctga ccatgtgtgc aagtcagggc ctgcgtgccg
    13861 gtatggtagc gggtgttatc gttaaccgca cccagcaaga gatcccgaat gctgagacga
    13921 tgaaacaaac cgaaagccat gcggtgaaaa tcgtggtgga agcggcgcgt cgtctgctgt
    13981 aattctcttc tcctgtctga aggccgacgc gttcggcctt ttgtattttt gcgtagcgcc
    14041 tcgcaggaaa tgcctttcca actggacgtt tgtacagcac aattctattt tgtgcgggta
    14101 agttgttgcg tcaggaggcg ttgtggattt ctcaatcatg gtttacgcag ttattgcgtt
    14161 ggtgggtgtg gcaattggct ggctgtttgc cagttatcaa catgcgcagc aaaaagccga
    14221 gcaattagct gaacgtgaag agatggtcgc ggagttaagc gcggcaaaac aacaaattac
    14281 ccaaagcgag cactggcgtg cagagtgtga attgctcaat aacgaagtgc gcagcctgca
    14341 aagtattaac acctctctgg aggccgatct gcgtgaagta accacgcgga tggaagccgc
    14401 acagcaacat gctgacgata aaattcgcca gatgattaac agcgagcagc gcctcagtga
    14461 gcagtttgaa aacctcgcca accgtatttt tgagcacagc aatcgccggg ttgatgagca
    14521 aaaccgtcag agtctgaaca gcctgttgtc gccgctacgt gaacaactgg acggtttccg
    14581 ccgtcaggtt caggacagct tcggtaaaga agcacaagaa cgccataccc tgacccacga
    14641 aattcgcaat ctccagcaac tcaacgcgca aatggcccag gaagcgatca acctgacgcg
    14701 cgcgctgaaa ggcgacaata aaacccaggg caactggggc gaggtagtat tgacgcgggt
    14761 gctggaggct tccggtctgc gtgaagggta tgaatatgaa acccaggtca gcatcgaaaa
    14821 tgacgcccgc tcgcggatgc agccggatgt catcgtgcgg ctgccgcagg gaaaagatgt
    14881 ggtgatcgac gccaaaatga cgctggtcgc ctatgaacgc tattttaacg ccgaagacga
    14941 ctacacccgc gaaagcgcgc tacaggaaca tatcgcgtcg gtgcgtaacc atatccgttt
    15001 gctgggacgc aaagattatc aacagctgcc ggggctgcga actctggatt acgtgctgat
    15061 gtttattccc gttgaacccg cttttttact ggcgcttgac cgccagccgg agctgatcac
    15121 cgaagcgttg aaaaacaata tcatgctggt tagcccgact acgctgctgg tggcgttgcg
    15181 tactattgcc aacctgtggc gttatgagca tcaaagccgt aacgcccagc aaatcgccga
    15241 tcgtgccagc aagctgtacg acaagatgcg tttgttcatc gatgacatgt ccgcgattgg
    15301 tcaaagtctc gacaaagcgc aggataacta ccgccaggca atgaaaaaac tctcttcagg
    15361 tcgcggaaat gtgctggcgc aggcagaagc gtttcgcggt ttaggagtag aaattaaacg
    15421 cgagattaat ccggatttgg ctgaacaggc ggtgagccag gatgaagagt atcgacttcg
    15481 gtcggttccg gagcagccga atgatgaagc ttatcaacgc gatgatgaat ataatcagca
    15541 gtcgcgctag cccattaggg gtagttaagc cgggtagaaa tctagggcat cgacgcccaa
    15601 tctgttacac ttctggaaca attttttgat gagcaggcat tgagatggtg gataagtcac
    15661 aagaaacgac gcactttggt tttcagaccg tcgcgaagga acaaaaagcg gatatggtcg
    15721 cccacgtttt ccattccgtg gcatcaaaat acgatgtcat gaatgatttg atgtcatttg
    15781 gtattcatcg tttgtggaag cgattcacga ttgattgcag cggcgtacgc cgtgggcaga
    15841 ccgtgctgga tctggctggt ggcaccggcg acctgacagc gaaattctcc cgcctggtcg
    15901 gagaaactgg caaagtggtc cttgctgata tcaatgaatc catgctcaaa atgggacgcg
    15961 agaagctgcg taatatcggt gtgattggca acgttgagta tgttcaggcg aacgctgagg
    16021 cgctgccgtt cccggataac acctttgatt gcatcaccat ttcgtttggt ctgcgtaacg
    16081 tcaccgacaa agataaagca ctgcgttcaa tgtatcgcgt gctgaaaccc ggcggccgcc
    16141 tgctggtgct tgagttctcg aagccaatta tcgagccgct gagcaaagcc tatgatgcat
    16201 actccttcca tgtgctgccg cgtattggct cactggtcgc gaacgacgcc gacagctacc
    16261 gttatctggc agaatccatc cgtatgcatc ccgatcagga taccctgaaa gccatgatgc
    16321 aggatgccgg attcgaaagt gtcgactact acaatctgac ggcaggggtt gtggcgctgc
    16381 atcgtggtta taagttctga caggagaccg gaaatgcctt ttaaaccttt agtgacggca
    16441 ggaattgaaa gtctgctcaa caccttcctg tatcgctcac ccgcgctgaa aacagcccgc
    16501 tcgcgtctgc tgggtaaagt attgcgcgtg gaggtaaaag gcttttcgac gtcattgatt
    16561 ctggtgttca gcgaacgcca ggttgatgta ctgggcgaat gggcaggcga tgctgactgc
    16621 accgttatcg cctacgccag tgtgttgccg aaacttcgcg atcgccagca gcttaccgca
    16681 ctgattcgca gtggtgagct ggaagtgcag ggcgatattc aggtggtgca aaacttcgtt
    16741 gcgctggcag atctggcaga gttcgaccct gcggaactgc tggcccctta taccggtgat
    16801 atcgccgctg aaggaatcag caaagccatg cgcggaggcg caaagttcct gcatcacggc
    16861 attaagcgcc agcaacgtta tgtggcggaa gccattactg aagagtggcg tatggcaccc
    16921 ggtccgcttg aagtggcctg gtttgcggaa gagacggctg ccgtcgagcg tgctgttgat
    16981 gccctgacca aacggctgga aaaactggag gctaaatgac gccaggtgaa gtacggcgcc
    17041 tatatttcat cattcgcact tttttaagct acggacttga tgaactgatc cccaaaatgc
    17101 gtatcaccct gccgctacgg ctatggcgat actcattatt ctggatgcca aatcggcata
    17161 aagacaaact tttaggtgag cgactacgac tggccctgca agaactgggg ccggtttgga
    17221 tcaagttcgg gcaaatgtta tcaacccgcc gcgatctttt tccaccgcat attgccgatc
    17281 agctggcgtt attgcaggac aaagttgctc cgtttgatgg caagctggcg aagcagcaga
    17341 ttgaagctgc aatgggcggc ttgccggtag aagcgtggtt tgacgatttt gaaatcaagc
    17401 cgctggcttc tgcttctatc gcccaggttc ataccgcgcg attgaaatcg aatggtaaag
    17461 aggtggtgat taaagtcatc cgcccggata ttttgccggt cattaaagcg gatctgaaac
    17521 ttatctaccg tctggctcgc tgggtgccgc gtttgctgcc ggatggtcgc cgtctgcgcc
    17581 caaccgaagt ggtgcgcgag tacgaaaaga ccttgattga tgaactgaat ttgctgcggg
    17641 aatctgccaa cgccattcag cttcggcgca attttgaaga cagcccgatg ctctacatcc
    17701 cggaagttta ccctgactat tgtagtgaag ggatgatggt gatggagcgc atttacggca
    17761 ttccggtgtc tgatgttgcg gcgctggaga aaaacggcac taacatgaaa ttgctggcgg
    17821 aacgcggcgt gcaggtgttc ttcactcagg tctttcgcga cagctttttc catgccgata
    17881 tgcaccctgg caacatcttc gtaagctatg aacacccgga aaacccgaaa tatatcggca
    17941 ttgattgcgg gattgttggc tcgctaaaca aagaagataa acgctatctg gcagaaaact
    18001 ttatcgcctt ctttaatcgc gactatcgca aagtggcaga gctacacgtc gattctggct
    18061 gggtgccacc agataccaac gttgaagagt tcgaatttgc cattcgtacg gtctgtgaac
    18121 ctatctttga gaaaccgctg gccgaaattt cgtttggaca tgtactgtta aatctgttta
    18181 atacggcgcg tcgcttcaat atggaagtgc agccgcaact ggtgttactc cagaaaaccc
    18241 tgctctatgt cgaaggggta ggacgccagc tttatccgca gctcgattta tggaaaacag
    18301 cgaagccttt cctggagtcg tggattaaag atcaggtcgg tattcctgcc ctggtgagag
    18361 catttaaaga aaaagcgccg ttctgggtcg aaaaaatgcc agaactgcct gaactggttt
    18421 acgacagttt gcgccagggc aagtatttac agcacagtgt tgataagatt gcccgcgagc
    18481 ttcagtcaaa tcatgtacgt cagggacaat cgcgttattt tctcggaatt ggcgctacgt
    18541 tagtattaag tggcacattc ttgttggtca gccgacctga gtgggggctg atgcccggct
    18601 ggttaatggc aggtggtctg atcgcctggt ttgtcggttg gcgcaaaaca cgctgatttt
    18661 ttcatcgctc aaggcgggcc gtgtaacgta taatgcggct ttgtttaatc atcatctacc
    18721 acagaggaac atgtatgggt ggtatcagta tttggcagtt attgattatt gccgtcatcg
    18781 ttgtactgct ttttggcacc aaaaagctcg gctccatcgg ttccgatctt ggtgcgtcga
    18841 tcaaaggctt taaaaaagca atgagcgatg atgaaccaaa gcaggataaa accagtcagg
    18901 atgctgattt tactgcgaaa actatcgccg ataagcaggc ggatacgaat caggaacagg
    18961 ctaaaacaga agacgcgaag cgccacgata aagagcaggt gtaatccgtg tttgatatcg
    19021 gttttagcga actgctattg gtgttcatca tcggcctcgt cgttctgggg ccgcaacgac
    19081 tgcctgtggc ggtaaaaacg gtagcgggct ggattcgcgc gttgcgttca ctggcgacaa
    19141 cggtgcagaa cgaactgacc caggagttaa aactccagga gtttcaggac agtctgaaaa
    19201 aggttgaaaa ggcgagcctc actaacctga cgcccgaact gaaagcgtcg atggatgaac
    19261 tacgccaggc cgcggagtcg atgaagcgtt cctacgttgc aaacgatcct gaaaaggcga
    19321 gcgatgaagc gcacaccatc cataacccgg tggtgaaaga taatgaagct gcgcatgagg
    19381 gcgtaacgcc tgccgctgca caaacgcagg ccagttcgcc ggaacagaag ccagaaacca
    19441 cgccagagcc ggtggtaaaa cctgctgcgg acgctgaacc gaaaaccgct gcaccttccc
    19501 cttcgtcgag tgataaaccg taaacatgtc tgtagaagat actcaaccgc ttatcacgca
    19561 tctgattgag ctgcgtaagc gtctgctgaa ctgcattatc gcggtgatcg tgatattcct
    19621 gtgtctggtc tatttcgcca atgacatcta tcacttggta tccgcgccat tgatcaagca
    19681 gttgccgcaa ggttcaacga tgatcgccac cgacgtggcc tcgccgttct ttacgccgat
    19741 caagctgacc tttatggtgt cgctgattct gtcagcgccg gtgattctct atcaggtgtg
    19801 ggcatttatc gccccagcgc tgtataagca tgaacgtcgc ctggtggtgc cgctgctggt
    19861 ttccagctct ctgctgtttt atatcggcat ggcattcgcc tactttgtgg tctttccgct
    19921 ggcatttggc ttccttgcca ataccgcgcc ggaaggggtg caggtatcca ccgacatcgc
    19981 cagctattta agcttcgtta tggcgctgtt tatggcgttt ggtgtctcct ttgaagtgcc
    20041 ggtggcaatt gtgctgctgt gctggatggg gattacctcg tcagaagact tacgcaaaaa
    20101 acgcccgtat gtgctggttg gtgcattcgt tgtcgggatg ttgctgacgc cgccggatgt
    20161 cttctcgcaa acgctattgg cgatcccgat gtactgtctg tttgaaatcg gtgtcttctt
    20221 ctcacgcttt tacgttggta aagggcgaaa tcgggaagag gaaaacgacg ctgaagcaga
    20281 aagcgaaaaa actgaagaat aaattcaacc gcccgtcagg gcggttgtca tatggagtac
    20341 aggatgtttg atatcggcgt taatttgacc agttcgcaat ttgcgaaaga ccgtgatgat
    20401 gttgtagcgc gcgcttttga cgcgggagtt aatgggctac tcatcaccgg cactaacctg
    20461 cgtgaaagcc agcaggcgca aaagctggcg cgtcagtatt cgtcctgttg gtcaacggcg
    20521 ggcgtacatc ctcacgacag cagccagtgg caagctgcga ctgaagaagc gattattgag
    20581 ctggccgcgc agccagaagt ggtggcgatt ggtgaatgtg gtctcgactt taaccgtaac
    20641 ttttcgacgc cggaagagca ggaacgcgct tttgttgctc agctacgcat tgccgcagaa
    20701 ttaaacatgc cggtatttat gcactgtcgc gatgcccacg agcggtttat gacattgctg
    20761 gagccgtggc tggataaact gcctggtgcg gttcttcatt gctttaccgg cacacgcgaa
    20821 gagatgcagg cgtgcgtggc gcgtggaatt tatatcggca ttaccggttg ggtttgcgat
    20881 gaacgacgcg ggctggagct gcgggaattg ttgccgttga ttccggcgga gaaattgctg
    20941 atcgaaacag atgcgccgta tctgctcccg cgcgatctca cgccaaagcc atcatcccgg
    21001 cgcaacgagc cagcccatct gccccatatt ttgcaacgta ttgcgcactg gcgtggagaa
    21061 gatgccgcat ggctggctgc caccacggat gccaatgtca aaacactgtt tgggattgcg
    21121 ttttagagtt tgcggaactc ggtgttcttc acactgtgct taatctcttt attaataaga
    21181 ttaagcaata gcatggagcg agcctcacca tcgggttcgg tgaaaatggc ctgaaagcct
    21241 tcgaacgcgc cttcggtaat aatcacctta tcacccggat aagggggggc cggatcgaca
    21301 atgtctttcg gtttatatac cgatagctga tgaattaccg ccgatgggac tatcgctggc
    21361 gacgcgccaa agcgcacaaa gtggctgacg ccacgcgtag cgctgatggt cgtggtatga
    21421 atcacttctg gatcaaattc cacaaacagg tagttaggga ataacggttc actgactgcg
    21481 gtacgtttac cccgtacgat tttttccagg gtgatcatcg gagcgaggca attaaccgcc
    21541 tgtctctcaa ggtgttcctg ggcacgttga agttgcccgc gcttgcagta cagtaaatac
    21601 caggattgca taatgactct tatccgttta atcggggcgc aaggatagca aaagctttac
    21661 gctaagttaa ttatattccc cggtttgcgt tataccgtca gagttcacgc taatttaaca
    21721 aatttacagc attgcaaaga tgaacgccgt ataatgggcg cagattaaga ggctacaatg
    21781 gacgccatga aatataacga tttacgcgac ttcctgacgc tgcttgaaca gcagggtgag
    21841 ctaaaacgta tcacgctccc ggtggatccg catctggaaa tcactgaaat tgctgaccgc
    21901 actctgcgtg ctggtgggcc tgcgctgttg ttcgaaaacc ctaaaggcta ctcaatgccg
    21961 gtgctgtgca acctgttcgg tacgccaaag cgcgtggcga tgggcatggg gcaggaagat
    22021 gtttcggcgc tgcgtgaagt tggtaaatta ttggcgtttc tgaaagagcc ggagccgcca
    22081 aaaggtttcc gcgacctgtt tgataaactg ccgcagttta agcaggtgtt aaacatgccg
    22141 acaaagcgac tgcgtggtgc gccctgccaa caaaaaatcg tctctggcga tgacgtcgat
    22201 ctcaatcgca ttcccattat gacctgctgg ccggaagatg ccgcgccgct gattacctgg
    22261 gggctgaccg taacgcgcgg cccgcataaa gagcggcaga atctgggcat ttatcgccag
    22321 cagctgattg gtaaaaacaa actgattatg cgctggctgt cgcatcgcgg cggcgcgctg
    22381 gattatcagg agtggtgtgc ggcgcatccg ggcgaacgtt tcccggtttc tgtggcgctg
    22441 ggtgccgatc ctgccacgat tctcggtgca gtcacccccg ttccggatac gctttcagag
    22501 tatgcgtttg ccggattgct acgcggcacc aaaaccgaag tagtaaagtg tatttccaat
    22561 gacctcgaag tgccagccag tgccgaaatc gtcctggaag ggtatatcga tcctggtgag
    22621 atggcgccgg aagggccgta tggtgaccac acaggttact ataatgaagt cgataatttc
    22681 ccggtgttta ccgtgacgca tattacccag cgtgaagatg cgatttacca ttccacctat
    22741 accgggcgtc cgccagatga gcccgcggta ctgggcgtgg cgttgaacga agtgtttgta
    22801 ccgattctgc aaaaacagtt cccggaaatt gtcgattttt acctgccgcc ggaaggctgt
    22861 tcttatcgtc tggcggtagt gacgatcaaa aaacagtacg ccggacacgc gaagcgcgtc
    22921 atgatgggcg tctggtcgtt cttacgccag tttatgtaca ctaaatttgt gatcgtttgc
    22981 gatgatgacg tcaacgcacg cgactggaac gatgtgattt gggcgattac cacccgtatg
    23041 gacccggcgc gggatactgt tctggtagaa aatacgccta ttgattatct ggattttgcc
    23101 tcgcctgtct ctgggctggg ttcaaaaatg gggctggatg ccacgaataa atggccgggg
    23161 gaaacccagc gtgaatgggg acgtcccatc aaaaaagatc cagatgttgt cgcgcatatt
    23221 gacgccatct gggatgaact ggctattttt aacaacggta aaagcgcctg atgcgcgttt
    23281 gttttgccct atttatcgat ccgacagaga aagcgcatga caaccttaag ctgtaaagtg
    23341 acctcggtag cagctatcac ggataccgta tatcgtgtcc gcatcgtgcc agacgcggcc
    23401 ttttcttttc gtgctggtca gtatttgatg gtagtgatgg atgagcgcga caaacgtccg
    23461 ttctcaatgg cctcgacgcc ggatgaaaaa gggtttatcg agctgcatat tggcgcttct
    23521 gaaatcaacc tttacgcgaa agcagtcatg gaccgcatcc tcaaagatca tcaaatcgtg
    23581 gtcgaccttc cccacggaga agcgtggctg cgcgatgatg aagagcgtcc gatgattttg
    23641 attgcgggcg gcaccgggtt ctcttatgcc cgctcgattt tgctgacagc gttggcgcgt
    23701 aacccaaacc gtgatatcac catttactgg ggcgggcgtg aagagcagca tctgtatgat
    23761 ctctgcgagc ttgaggcgct ttcgttaaag caccttggtc tgcaagtggt gccggtggtt
    23821 gaacaaccgg aagcaggctg gcgtgggcgt actggtaccg tgttaacggc ggtattgcag
    23881 gatcatggta cgctggcaga gcatgatatc tatattgccg gacgttttga gatggcgaaa
    23941 attgcccgtg acctgttttg cagtgagcgt aatgcgcggg aagatcgcct gtttggcgat
    24001 gcgtttgcat ttatctgaga tataaaaaaa cccgcccctg acaggcggga agaacggcaa
    24061 ctaaaacttt attcagtggc atttagatct atgtcgcatc cggcaattgg tgcacgatgc
    24121 ctgatgcgac gctggcgcgt cttatcaggc ctacattggt gccggatcgg tagaccggat
    24181 aaggcgttca cgccgcatcc ggcaagtggt taaacccgct caaacaccgt cgcaataccc
    24241 tgacccagac cgatacacat cgtcgccaga ccaaactgaa cgtctttgcg ttccatcaga
    24301 ttcagcagcg tggtgctgat acgcgcaccg gaacaaccca gcggatgacc cagcgcgatc
    24361 gcaccaccat tgaggttgat cttctcgtca atctgctcca ttagcccgag atctttaatg
    24421 cacggcagga tctgcgcggc aaaggcttcg ttcatctcaa acacgccgat atcgctggca
    24481 gaaagccccg cttttttcag cgccagcttc gaggccggaa ccgggccgta acccataatc
    24541 gatgggtcac aaccaacgac cgccatcgaa cgcacacgag cgcgcggctt aagacctaat
    24601 tcatgggcgc ggctttcact catcaccagc atggcagctg cgccatcgga aagtgcagaa
    24661 gatgtgcccg ccgttaccgt accgtttact ggatcaaacg ccggacgcag cgtggcgagg
    24721 gcttccacgg tggtttccgg gcgaatcact tcgtcgtaat taaactgctt caggacgccg
    24781 tcggcatcgt gaccaccagt tgggatgatt tcatttttaa atgcggccga ctgcgtggcg
    24841 gcccaggcgc gggcgtgtga ccgcgcggca aaggcatcct gcatttcacg gctgataccg
    24901 tgcatacgcg ccagcatttc tgccgttaag cccatcatgc ccgccgcttt ggcgacattg
    24961 cggctcaggc cggggtgaaa atcgacgccg tgactcatcg gcacatggcc tatatgctcc
    25021 acgccgccaa ccagacatgc ctgcgtatcg ccagtcatga tcattcgtgc tgcgtcatgc
    25081 agtgcctgca tggatgaacc acacaagcga ttgacggtaa ccgccgggac agagtgcggc
    25141 acttctgcca gcagcgccgc gttacgggcg atgttaaaac cctgctccag cgtctgttgc
    25201 acacaacccc agtaaatatc gtcgagggcc gccgcttcca gcgccgggtt acgcgccagc
    25261 aggctacgca ttaaatgagc ggagagatct tccgcacgca cgttacgaaa agcaccgccc
    25321 ttcgaacggc ccatcggggt gcgaattgca tcgacaatga caacctgttc cattgtgact
    25381 ccttaagccg ttttcaggtc gccaaccgga cgggctggct caaccggagg atagtacggt
    25441 tcgttatgac gcgctttatt acgcagacct tccggcactt catacagcgg gccgaggtgc
    25501 tgatattgct gtgccatatc gaggtatttt gcgctaccga gggtgtccag ccagcggaac
    25561 gcgccgccgt ggaacggagg gaagcccagg ccgtagacca gcgccatatc cgcttccgcc
    25621 ggagtggcga taatgccctc ttccagacag cgcaccactt cgttgaccat cgggatcatc
    25681 atgcgggcga taatctcttc ttcgctgaaa tcgcgcttcg gttggctcac ttctgccagc
    25741 aggtcttcaa cggcggcgtc ttcttctttc ttcggcttac ctttgctgtc ttctttataa
    25801 cgccagaaac cgaggccgtt cttctgacca aagcggttgg catcaaacag cgcgtcgatg
    25861 gcatcgcggt aatctttctg catccgctgc gggaagcctg ctgccatgac agcctgagcg
    25921 tgatgcgcgg tatcaatgcc cacaacgtcc agcagatatg ccgggcccat cggccagcca
    25981 aactgttttt ccatcacttt gtcgatcttg cggaaatccg cgccgtcgcg cagcagctgg
    26041 ctgaaaccgg cgaaatacgg gaacagcacg cggttaacaa agaagccggg gcagtcgtta
    26101 accacaatcg gcgtcttgcc catcttgctc gcccaggcga caactttcgc gatggtttcg
    26161 tcggagcttt tctcgccgcg aataatttct accaacggca ttcggtggac cgggttaaag
    26221 aagtgcatcc cgcagaagtt ttccgggcgt tccagcgcgt tggccagttc gctgatagga
    26281 atggttgaag tgttagacgc cagcacggta tcctggcgta ctttttgttc ggtttctgcc
    26341 agtacggctt ttttcacttt cgggttttca acaaccgctt ctaccacaat atccacgcgg
    26401 tcaaatccgg cgtagtcgag cgttgggtgg atcgtggaga tcacgccagc cagtttcaga
    26461 ccatcgattt tgccgcgctc aagctgcttg ttcagcagtt tcgcggcttc ggtcatgccg
    26521 agggttaacg acttgtcgtt gatatctttc atgacaaccg gcacgccttt ccacgcggac
    26581 tggtaagcga taccgccacc catgatgcct gcgcccagca cagcagcctg tttcggggtt
    26641 tcaacgtctt tggtgagttt cttcgctttg ccttttacat attgatcgtt aaggaaaatg
    26701 ccgaccagtg cgcgagcttc gttggtatgc gccagcggga caaaactttt gttttccaga
    26761 tttaaggctt cttcacgacc aaaacgggcc gcagcttcaa tggtttttac tgcggtgatg
    26821 ggggccggat aatgtttccc cgccgtttgc gcgaccattc ctttagcgat ggtgaagctc
    26881 atggtggctt caatcttgct cagttttagc ggttcctgct tcggctgacg tttcgctttc
    26941 cagtcgaggt cgccgttaat ggcctggcgt aaaatcgcca tcgccccttc aatcagtttt
    27001 tctgctttga ctacgccatc caccagaccg attttcagcg cctgatccgc gccgacatct
    27061 ttaccggcgg caatgatttc cagtgcactg tcagcgccca gcatacgtgg catacgtaca
    27121 gaaccgccaa agcccggcat gatgcccagt ttggtttccg gcagaccgat gcgcagatcc
    27181 ggcgtcgcca gacgataatc ggtcgccagc acgcattcgc agccaccacc cagcgcatag
    27241 ccgttgacgg cagcaatggt cggcaccggc agatcttcca ggcgattaaa cacgctattg
    27301 gcaaagtgca gccactgact taactgttct tcaggaacga ggaacaggga caaaaattcg
    27361 gtgatatcag caccgacgat aaaggctgct ttgttcgaac gcagcagcag cccttttaga
    27421 tctgattgct gttccagcac gccgatggcc tcgccgaggc tggcgacggt cgcagtgtcg
    27481 agtttattaa ctgaacctgg ggcatcaaat accagttcgg caatgccatc ttccagccag
    27541 tcaaggtaca gggtgtcgcc tttgtaaagc atgtcagtct cctgaatccg caaggtaatc
    27601 tggtcgtacc agatgcatcg gagtgtgtgt tttgtgttaa aaatatgcaa ataaaagatt
    27661 aaagaaatgc cgatctgatc acgctcggca gaaatcacgc tctggatgaa cgatgtgcta
    27721 agatgcggag acttaaggtc aaaaaaacag aagggtaaaa aatggaatca ctggcctcgc
    27781 tctataaaaa tcatatagct accttacaag aacggactcg cgatgcgctg gcgcgcttca
    27841 agctggatgc attacttatt cactccggcg agctgttcaa cgtttttctc gacgatcatc
    27901 cctatccgtt taaagtgaac ccgcaattca aagcgtgggt gccggtaact caggtgccaa
    27961 actgctggtt gctggtggat ggcgtgaaca agccgaaact gtggttctat ctgccggttg
    28021 attactggca caacgtcgaa ccgctgccga cctccttctg gactgaagat gtggaagtga
    28081 tcgcgctgcc gaaagccgat ggcattggta gcctgctgcc tgctgcgcgc ggcaatatcg
    28141 gttatatcgg tccggtgccg gaacgtgcgc tgcaactggg tattgaggcc agcaacatca
    28201 acccgaaagg ggtgatcgac tacctgcatt attaccgctc cttcaaaacc gagtacgaac
    28261 tggcctgtat gcgtgaagcg cagaaaatgg cggtcaatgg tcatcgtgcg gcagaagaag
    28321 cgttccgttc tggcatgagc gagttcgata tcaatattgc ctatctgacc gcgaccggtc
    28381 atcgtgatac cgacgtacct tacagcaaca ttgtggcgct taacgaacac gctgcggtgt
    28441 tgcattacac caaactggac caccaggcac cggaagagat gcgcagcttc ctgctggatg
    28501 ccggggccga atataacggc tatgcggctg acctgacccg tacctggtcg gcaaaaagcg
    28561 acaacgacta cgcacagctg gtgaaagacg taaatgatga acaactggcg ctgatcgcca
    28621 ccatgaaagc aggcgtcagc tatgtggatt accacatcca gttccatcag cgcatcgcca
    28681 aactgctgcg taaacatcaa atcatcaccg atatgagtga agaagcgatg gtcgaaaacg
    28741 atctcaccgg accgtttatg ccgcacggta ttggccatcc gctgggcctg caggtgcatg
    28801 acgtcgccgg ttttatgcag gatgatagcg gtacacacct cgcggcaccg gcaaaatatc
    28861 cgtacctgcg ctacacccgt attctccagc cgggcatggt gttaaccatc gaaccgggta
    28921 tctacttcat tgaatcgctt ctggcaccgt ggcgtgaagg gcagttcagc aagcacttca
    28981 actggcagaa aattgaagca ctgaaaccgt tcggcggcat tcgtatcgaa gacaacgtgg
    29041 tgatccacga aaacaacgtg gaaaacatga cccgggatct gaaactggcg tgatggaaag
    29101 ctggttaatt cctgcggcac cggtcacggt cgttgaagag atcaaaaaga gccgcttcat
    29161 aacgatgttg gcgcataccg atggcgttga ggcggcgaaa gcgtttgttg aatcggtgcg
    29221 ggcagaacac cccgatgccc gccaccattg cgtggcgtgg gtggcgggtg cgccggatga
    29281 ttctcaacag ctgggtttct ctgacgacgg ggagccggcg ggaacggcag gtaaaccgat
    29341 gctcgcccag ctaatgggca gcggcgtcgg ggaaattacc gctgtggtag tgcgctacta
    29401 cggcggcata ttgcttggca ccggtgggtt agtgaaagcg tatggcggcg gcgtgaatca
    29461 ggcgctgcgc cagctaacga cccaacgcaa gacgccatta accgaatata ctttgcaatg
    29521 tgaatatagt cagttaaccg gcattgaagc gttgctgggg cagtgtgacg gcaaaattat
    29581 caacagtgat tatcaggcat tcgttctgct gcgggtggcg cttccggcgg cgaaagtggc
    29641 tgaattttcc gcaaagctgg cggattttag ccgtggttca ttgcaattgt tagcgattga
    29701 agaataatcc ccacttcgtt ttgcagaact aaggaagcgg cagagatgca ttttcgcgcc
    29761 attacccgaa tcgttggact actggtcatc ttattttcag ggaccatgat tatccctggg
    29821 ctggtagcac tcatctaccg ggatggagcg ggccgcgctt ttacccagac cttttttgtc
    29881 gccctcgcca ttggctctat gctgtggtgg ccgaaccgca aagagaaggg cgaactgaaa
    29941 tcccgtgagg ggtttctgat agtggtgctg ttttggaccg tgctgggtag tgtcggtgcg
    30001 ctccctttta tcttctcgga aagcccgaac ctcacgatta ccgatgcgtt ttttgaatct
    30061 ttctctggcc tgaccaccac cggggccact acgctggtgg ggctggattc gctccctcat
    30121 gccatcctct tttatcgcca gatgctgcaa tggtttggcg ggatggggat catcgtgtta
    30181 gcggttgcga tactgcctat cctcggcgtg ggtgggatgc agctctatcg cgcagaaatg
    30241 cccggcccgc tgaaagataa caaaatgcgc ccgcgaattg cggaaacggc gaaaactctg
    30301 tggttgattt atgtcttgct gaccgtcgcc tgtgcgctgg cgttgtggtt tgccggaatg
    30361 gatgcctttg atgccatcgg ccatagcttt gcgactatcg ctattggcgg cttctcgaca
    30421 catgatgcca gtatcggtta tttcgacagt ccgactatta acactatcat tgctatcttc
    30481 ctgctgatct ccggctgtaa ctacggtctg cacttttcac tgttaagtgg gcgtagtctg
    30541 aaggtttatt ggcgcgatcc ggaatttcgc atgtttatcg gcgtacagtt tacgctggtg
    30601 gttatttgta cactcgtact gtggtttcat aatgtctaca gttcggcgct gatgacaatt
    30661 aaccaggcgt ttttccaggt ggtatcgatg gcgacaaccg ccgggtttac gactgacagc
    30721 attgcccgct ggccgctctt tttgccggta ctgcttttat gttcagcttt tatcggcggt
    30781 tgcgccgggt caacgggcgg tggcctgaaa gtgatccgca tcctgctgct gtttaagcag
    30841 gggaaccgtg agctgaaacg actggtgcat ccgaacgccg tctatagcat taagctgggg
    30901 aatcgcgcac tgccggaacg tatcctcgaa gccgtttggg gatttttctc cgcctatgca
    30961 ttggtgttta ttgtcagtat gctggcgatt atcgccacgg gcgtggatga tttttctgcc
    31021 tttgcctcag ttgttgcgac attgaataac ctggggccag ggcttggcgt ggttgctgat
    31081 aactttacca gtatgaaccc ggtggctaaa tggatcctga ttgccaacat gctgtttggt
    31141 cgtctcgagg tctttacatt actggtgctc tttaccccga ctttctggcg tgaatgatgg
    31201 agtaatacgt gaaaacatta attcttttct caacaaggga cggacaaacg cgcgagattg
    31261 cctcctacct ggcttcggaa ctgaaagaac tggggatcca gacggatgtc gccaatgtgc
    31321 accgcattga agaaccacaa tgggaaaact atgaccgtgt ggtcattggt gcttctattc
    31381 gctatggtca ctaccactct gcgttccagg aatttgtcaa aaaacatgcg acgcgactga
    31441 attcgatgcc gagcgccttt tattccgtaa acctggtggc gcgcaaaccg gagaagcgta
    31501 ctccacagac caacagctac gcgcggaagt ttctgatgaa ctcgcaatgg cgtcccgatc
    31561 gctgcgcggt cattgccggg gcgctgcgtt acccacgtta tcgctggtac gaccgtttta
    31621 tgctcaagct gattatgaag atgtcaggcg gtgaaacgga tacgcgcaaa gaagttgtct
    31681 ataccgattg ggagcaggtg gcgaatttcg cccgagaaat cgcccattta accgacaaac
    31741 cgacgctgaa ataagcataa agaataaaaa atgcgcggtc agaaaattat tttaaatttc
    31801 ctcttgtcag gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa
    31861 acacgcagcc gggtcagcgg ggttctcctg agaattccga cagagaaagc aaaaataaat
    31921 gcttgacttt gtagcgggaa agcgtattat gaataccccg cgccg
//
LOCUS       DAZSNO010000032        30907 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZSNO010000032 DAZSNO010000000
VERSION     DAZSNO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30907
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.032"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            109..1767
                     /locus_tag="ACHWN2_001440"
     CDS             109..1767
                     /locus_tag="ACHWN2_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR8931034.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1764..2720)
                     /locus_tag="ACHWN2_001441"
     CDS             complement(1764..2720)
                     /locus_tag="ACHWN2_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR8931035.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2871..3986
                     /gene="macA"
                     /locus_tag="ACHWN2_001442"
     CDS             2871..3986
                     /gene="macA"
                     /locus_tag="ACHWN2_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR8931036.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3983..5929
                     /gene="macB"
                     /locus_tag="ACHWN2_001443"
     CDS             3983..5929
                     /gene="macB"
                     /locus_tag="ACHWN2_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR8931037.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6002..6226)
                     /gene="cspD"
                     /locus_tag="ACHWN2_001444"
     CDS             complement(6002..6226)
                     /gene="cspD"
                     /locus_tag="ACHWN2_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR8931038.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6376..6450
                     /gene="yljB"
                     /locus_tag="ACHWN2_001445"
     CDS             6376..6450
                     /gene="yljB"
                     /locus_tag="ACHWN2_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR8931039.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6549..6869
                     /gene="clpS"
                     /locus_tag="ACHWN2_001446"
     CDS             6549..6869
                     /gene="clpS"
                     /locus_tag="ACHWN2_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR8931040.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6900..9176
                     /gene="clpA"
                     /locus_tag="ACHWN2_001447"
     CDS             6900..9176
                     /gene="clpA"
                     /locus_tag="ACHWN2_001447"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR8931041.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9579..9666)
                     /locus_tag="ACHWN2_001448"
     tRNA            complement(9579..9666)
                     /locus_tag="ACHWN2_001448"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9630..9632),aa:Ser,seq:gga)
     gene            complement(9920..10138)
                     /gene="infA"
                     /locus_tag="ACHWN2_001449"
     CDS             complement(9920..10138)
                     /gene="infA"
                     /locus_tag="ACHWN2_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR8931042.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10423..11127)
                     /gene="aat"
                     /locus_tag="ACHWN2_001450"
     CDS             complement(10423..11127)
                     /gene="aat"
                     /locus_tag="ACHWN2_001450"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR8931043.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPDDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11169..12890)
                     /gene="cydC"
                     /locus_tag="ACHWN2_001451"
     CDS             complement(11169..12890)
                     /gene="cydC"
                     /locus_tag="ACHWN2_001451"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR8931044.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12891..14657)
                     /gene="cydD"
                     /locus_tag="ACHWN2_001452"
     CDS             complement(12891..14657)
                     /gene="cydD"
                     /locus_tag="ACHWN2_001452"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR8931045.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14780..15745)
                     /gene="trxB"
                     /locus_tag="ACHWN2_001453"
     CDS             complement(14780..15745)
                     /gene="trxB"
                     /locus_tag="ACHWN2_001453"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR8931046.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16289..16783
                     /gene="lrp"
                     /locus_tag="ACHWN2_001454"
     CDS             16289..16783
                     /gene="lrp"
                     /locus_tag="ACHWN2_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR8931047.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16918..20946
                     /gene="ftsK"
                     /locus_tag="ACHWN2_001455"
     CDS             16918..20946
                     /gene="ftsK"
                     /locus_tag="ACHWN2_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR8931048.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21101..21712
                     /gene="lolA"
                     /locus_tag="ACHWN2_001456"
     CDS             21101..21712
                     /gene="lolA"
                     /locus_tag="ACHWN2_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR8931049.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21723..23066
                     /gene="rarA"
                     /locus_tag="ACHWN2_001457"
                     /gene_synonym="mgsA"
     CDS             21723..23066
                     /gene="rarA"
                     /locus_tag="ACHWN2_001457"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR8931050.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23157..24449
                     /gene="serS"
                     /locus_tag="ACHWN2_001458"
     CDS             23157..24449
                     /gene="serS"
                     /locus_tag="ACHWN2_001458"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR8931051.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24689..27132
                     /gene="dmsA"
                     /locus_tag="ACHWN2_001459"
                     /pseudo
     CDS             24689..27132
                     /gene="dmsA"
                     /locus_tag="ACHWN2_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27143..27760
                     /gene="dmsB"
                     /locus_tag="ACHWN2_001460"
     CDS             27143..27760
                     /gene="dmsB"
                     /locus_tag="ACHWN2_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR8931052.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27762..28553
                     /locus_tag="ACHWN2_001461"
     CDS             27762..28553
                     /locus_tag="ACHWN2_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFR8931053.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28589..29215)
                     /gene="ycaC"
                     /locus_tag="ACHWN2_001462"
     CDS             complement(28589..29215)
                     /gene="ycaC"
                     /locus_tag="ACHWN2_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR8931054.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29530..30678
                     /locus_tag="ACHWN2_001463"
     CDS             29530..30678
                     /locus_tag="ACHWN2_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8931055.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30696..>30907
                     /locus_tag="ACHWN2_001464"
     CDS             30696..>30907
                     /locus_tag="ACHWN2_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8931056.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         7602 a         7602 c         8312 g         7391 t
ORIGIN      
        1 atttttcaga caattgccat gcttaaatgt gatgtcatca cgtattagca aggcctttcc
       61 cgttatactg cgagcgtaaa ggataagtca catatttctg gaggggatat gattcttgag
      121 cgcgttgaaa ttgtgggttt tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac
      181 aacgtcctga ttggggagaa cgcgtggggt aaatccagct tgctggacgc cttaaccctg
      241 ctgctatcgc cagaatcaga tctctaccat tttgagcgcg acgatttctg gttcccgccg
      301 ggagatatca acgggcgaga acatcatctg catattattt tgaccttccg cgaatcgctg
      361 ccaggccgac atcgggttcg ccgttatcgg ccgctggaag cgtgctggac gccatgcacc
      421 gatggctatc accgtatttt ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg
      481 atgacactgc gcagttttct cgataaagac ggacatccga ttgatgttga ggatattaac
      541 gatcaggcac gccatctggt gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt
      601 atgcgccgta ttcgtaacgg cacggtgcca aatgtcccca atgtggaagt caccgcgcgc
      661 cagctcgatt tcctcgcccg tgagttgtcc tcacatccgc aaaatctctc tgatggacag
      721 attcgtcagg gactttccgc aatggtgcag ttgcttgagc attatttctc tgagcagggg
      781 gctggacagg cgcgatatcg tttaatgcgg cggcgggcca gcaatgagca acgaagctgg
      841 cgctatctgg acatcatcaa ccggatgatt gaccgacctg gagggcgctc gtatcgggtt
      901 attttgctcg gcctgtttgc tactttattg caggcaaaag gcacattgcg actggataaa
      961 gacgcgcgtc cgttattgct aattgaagat ccagaaaccc gcctgcatcc gattatgctt
     1021 tccgttgcct ggcatctgtt gaatcttttg cctttacagc gtattgctac caccaactct
     1081 ggcgagctgc tttcgttaac tccagtggaa catgtttgcc gcctggtgcg tgaatcttct
     1141 cgtgttgccg cctggcgtct ggggccaagt ggcttgagca ccgaagacag ccgccgcatc
     1201 tcttttcaca ttcgttttaa tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg
     1261 gaaggggaaa cggaaacctg ggttatcaat gaactggcgc gtcagtgcgg acatcatttt
     1321 gatgccgaag ggatcaaggt cattgagttt gcccagtccg ggctaaagcc actggttaaa
     1381 tttgcccgcc gaatggggat tgaatggcat gtactggtcg atggcgatga agcagggaag
     1441 aaatatgccg ctacggtacg cagcctgttg aataacgatc gggaagccga acgagaacat
     1501 ttaacggcgt taccggcgct ggatatggaa cattttatgt atcgccaggg attttccgat
     1561 gtgttccacc gcgtggcgca aatcccggaa aatgtaccga tgaatctacg caaaattatc
     1621 tcgaaagcga tccatcgctc ttccaaaccc gatcttgcca ttgaagtggc aatggaggca
     1681 ggacgtcgtg gtgtggactc cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg
     1741 tggctggcgc gcggtcgcgc ggattaaccg cgaaacatcg tggccatttg tggctgaata
     1801 gcgtcgagca tctcatagcg ccgacggtat tcagcccgtt ttttactggc gatttcggca
     1861 atctcttttc gtgctatctg tgctggaagg cggtaatggc gttcagcatc acatacgccg
     1921 ccaaccgact cccagaacgc gttgtaatca gcatggatct tgccttcttt atcgcgataa
     1981 cgcaggctgc ggtaaatatg cgtttcattg ctgacggcaa taatctgctc tacctgcaaa
     2041 cgttgggcaa acagacaggc cgcttccatc acgaggcgtt tgggaaatag cccgtggcag
     2101 gctttcgtcg cattctggat ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg
     2161 ccaataaaca tcgttctttt cccctgatat tcacacaggg taaacgtgat ctctgccaga
     2221 ggaatacctt cgctgttgcg gaacaggatt gtgctgtcac cttctttatc cattgagatc
     2281 atcatggtca gctcaagcgt gaactgctca ccgtttttgc cttccagttt agccagttgc
     2341 agcccggggg tattcaaata taagctgaat tcttccgccg acatacaccc gcggagtaac
     2401 gcataatggt aacgtaacgc ctccagcaat tgcttacggc taagattcgc ggcaaggtaa
     2461 gggcgatgca gacgcacagg cagtcgcggc tggcgcgtta acaacacatt gagattaggc
     2521 cagtgggaaa gttcgttcat ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc
     2581 aaaaatttct ggcgaaaact acagcgatgc cagaatttac ccggccgaca ctgtccacgt
     2641 gccagactaa gaaaaagtga caggctgctg agagattcag atggcgtaaa ggtccgttca
     2701 gttagctgcg acatattcat gaaatcaatg gttatacatg acgtcgattt caccattgcg
     2761 tatcttaacc aaacatcaat agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat
     2821 ttcgttgaag ttaatgaccc ggattggcat atggagtatt cagaatattt atgaaaaagc
     2881 ggaaaaccgt gaagaagcgt tacgttattg cgctggtgat agtcatcgcc ggactgatta
     2941 cgttatggag aattcttaac gcacccgtgc cgacttatca gacactgatt gtgcgccccg
     3001 gtgatttaca gcaaagcgtg ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg
     3061 tgggcgcgca ggtcagcggt cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa
     3121 aaaaagacca gcttttaggg gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg
     3181 tcgaagcaac gctgatggag ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac
     3241 tggcgcgggt gacgtattcc cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc
     3301 aggatctcga taccgccgcg acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg
     3361 acgcgcaaat caagcgcaat caggcctctc tcgatacggc taaaaccaat ctcgattaca
     3421 cccgtatcgt tgccccgatg gctggggaag tgacgcaaat caccactctg caaggccaga
     3481 cggtgattgc cgcacagcaa gcaccgaaca ttctgacgct ggcagatatg agcactatgc
     3541 tggtaaaagc gcaggtttct gaagcggatg taatccacct gaagccgggg caaaaagcct
     3601 ggtttacggt gcttggcgat ccactgacgc gctacgaggg gcaaatcaag gatgtgctac
     3661 cgacgccgga aaaggtaaac gacgctattt tctattacgc ccgttttgaa gtacccaacc
     3721 ccaatggttt gctgcggctg gatatgaccg cgcaagtgca tattcagctc accgatgtga
     3781 aaaatgtgtt gacgatccct ctgtcggcgt taggcgatcc ggttggcgat aatcgttata
     3841 aagtcaaatt gttgcgtaat ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta
     3901 acgataccga tgttgagatt gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg
     3961 aggccaaacc aggagctgca caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag
     4021 ctatcctgcc ggtgatgagc aggttgaggt gctgaagggc atcagcctca atatttatgc
     4081 gggtgagatg gtcgcgattg ttggcgcttc gggttccggt aaatcgaccc tgatgaatat
     4141 tctcggctgc ctggataagg ccaccagcgg cacctatcgc gtagccggtc aggatattgc
     4201 cacgctggac gccgatgcgc tggcgcaact gcgccgcgag catttcggct ttattttcca
     4261 gcgttaccat ttgcttccgc atttaaccgt cgagcagaac gttgaagtac ccgccgtcta
     4321 tgccggtctt gagcggaaac agcgactgct tcgtgcccag gagttgctgc aacggctggg
     4381 gctggaagac cgtacagagt attatccggc acagctttcg ggtggtcagc aacagcgcgt
     4441 cagcatcgcg cgggcattga tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg
     4501 cgcactggac agccattctg gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg
     4561 cgggcatacg gtgattatcg tcacccacga tccgcaggtc gctgctcagg ccgagcgggt
     4621 gatcgaaatt cgcgacggcg aaattgtgcg caatcctccc gccattgaaa aagtgaatgt
     4681 tgctggcggg acggagcctg ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg
     4741 ttttaacgag gcgctgacga tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt
     4801 actgaccatg ctggggatta ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg
     4861 tgacgccgcc aaacaaatgg tgctggcgga tattcgttct attggtacga atactattga
     4921 tgtctatccc gggaaagatt ttggcgatga cgatccgcaa tatcagcagg cgctgaagta
     4981 cgacgactta atcgccatcc aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc
     5041 gcaaaacctg cgcctgcgtt ataacaatgt tgatgttgct gccagtgcca atggcgtgag
     5101 cggcgattat tttaatgtct atggcatgac cttcagtgaa ggaaacacct ttaatcagga
     5161 gcagctgaac ggtcgtgcgc aggtcgtggt tctcgacagt aatactcgcc gccagctttt
     5221 cccccataaa gcagatgtgg ttggcgaggt gattctggtc ggcaatatgc ccgccagagt
     5281 cattggtgtg gcggaagaaa aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg
     5341 gctaccttac agcacgatgt ccgggcgagt tatgggccag tcgtggctta actccattac
     5401 tgtcagggtg aaagaaggat ttgacagcgc cgaggcggaa cagcaactca cgcgtttact
     5461 ttcactgcgc cacggtaaga aggatttctt tacctggaac atggacggcg tcttgaaaac
     5521 tgttgaaaag accacacgta ctttacaact gtttctgacg ctggtggcgg tgatttcgct
     5581 ggtggtgggc ggtattggtg taatgaatat tatgctggtg tcagtgaccg agcggacgcg
     5641 ggaaattggc attcgcatgg ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct
     5701 gatcgaagcc gtactggttt gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt
     5761 aattgctttc accttgcagc ttttcttacc cggctgggag attggttttt caccgttggc
     5821 gctgctgctg gcgtttctct gctcgacggc caccgggatt ttatttggct ggttacccgc
     5881 acgaaatgcg gcacgactgg atccagtaga tgctctggca cgggagtaat ttttgagata
     5941 aaaatgccag ccgatcgggc tggcattttg cctttaggat gtacacaatg agacagaaga
     6001 gctatgcgac tgccgcttct acttcgacgg gcacaataac actggcgtga ttgccttttg
     6061 gcccctggtg gacatcaaac tgaacggatt gtccagcttt tagcgttctg taaccatcca
     6121 tctgaatggt ggaataatga gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac
     6181 caaacccttt ggcattgttg aaccacttaa cagtaccctt ttccatgctt cgacatcctt
     6241 cgcaaatctt atacaagtaa gatggaataa accggggtca gagagggggc tgttcaaaac
     6301 ctcgccaact ctagaaatac aatttagaga attagggcga gccgtcaagc atttgacagg
     6361 ggccaggggg caggtatgaa tcaaaaattt gaagcagtta acgctattga caggaatgtg
     6421 acagatgtcg ctgatgccaa cgatagatga tagttatcta tcatgtggag tagattggtc
     6481 aggcaaataa gctcttgtca gcggcagggc gttctgccga taaccgtaac cgaagatgat
     6541 aactgacaat gggtaaaacg aacgactggc tggactttga tcaactggcg gaagaaaaag
     6601 ttcgcgacgc gctaaaaccg ccatctatgt ataaagtgat attagtcaat gatgattaca
     6661 ctccgatgga gtttgttatt gacgtgttac aaaaattctt ttcttatgat gtagaacgtg
     6721 caacgcaatt gatgctcgct gttcactacc aggggaaggc catttgcgga gtctttaccg
     6781 ccgaggttgc agaaaccaaa gtggcgatgg tgaacaagta cgcgagggag aatgagcatc
     6841 cattgctgtg tacgctagaa aaagcctgaa tgcaggcata aaaattgggg gaggtgccta
     6901 tgctcaatca agaactggaa ctcagtttaa atatggcttt cgccagagcg cgcgagcacc
     6961 gtcatgagtt tatgaccgtc gagcacttgt tactggcgct gctcagtaac ccatctgccc
     7021 gggaggcgct ggaagcgtgt tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct
     7081 ttattgaaca aaccacaccc gttctgcctg ccagtgaaga ggagcgcgac acacagccga
     7141 cgctgagttt tcagcgtgta ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca
     7201 atgaggttac cggtgcaaac gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg
     7261 catatctgtt gcgtaaacat gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg
     7321 gcacgcgtaa agacgagccg acacagtctt ctgatcctgg cagccagcca aacagcgaag
     7381 aacaagctgg tggggaggaa cgtatggaga atttcacgac gaacctgaac cagcttgcgc
     7441 gtgtgggcgg aatcgaccca ctaattggtc gtgagaaaga gctggagcgt gctattcagg
     7501 ttctctgccg tcgccgtaaa aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa
     7561 ccgcgattgc agaaggtctt gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg
     7621 ctgactgtac gatttactct ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg
     7681 gcgactttga aaaacgtttt aaagcgttgc tcaagcagct ggagcaggac actaacagca
     7741 tcctgtttat tgatgagatc cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg
     7801 tcgatgcggc taacctaatc aaaccgttgc tctccagcgg taaaattcgc gtaattggtt
     7861 cgacaaccta tcaggagttc agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct
     7921 tccagaaaat tgatattact gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc
     7981 tgaaaccgaa gtatgaagcg caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg
     8041 cggtagagct ggcggtgaaa tacattaacg atcgtcatct gccggataaa gccattgatg
     8101 ttatcgacga agcaggcgct cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg
     8161 ttaatgtggc ggatattgag tccgtggtgg cccgtattgc acgcattcca gagaagagtg
     8221 tttctcagag tgaccgcgat accctgaaaa aactcagcga tcgcctgaaa atgctggtct
     8281 tcggccagga taaagccatt gaggcgctga ctgaagccat taagatggcg cgtgcaggtt
     8341 taggtcacga acataaaccg gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga
     8401 aaacagaggt gacggtacag ctttcgaaag cgttgggcat tgagcttctg cgctttgata
     8461 tgtccgagta tatggaacgc cataccgtca gccgtctgat tggtgcgcct ccgggatacg
     8521 ttggttttga tcagggcggt ttgctgaccg atgcggtaat caagcatcca catgcggtgc
     8581 tgctgctgga cgaaatcgag aaagcgcatc cggacgtgtt caatattctg ttgcaggtga
     8641 tggacaacgg tacgctgacc gataacaacg gacgcaaagc ggacttccgt aacgtggtgc
     8701 tggtgatgac cactaacgcc ggggtacggg aaactgagcg caaatccatt ggtcttatcc
     8761 accaggataa cagcaccgat gcgatggaag agatcaagaa gatctttaca ccggaattcc
     8821 gtaaccgtct cgacaacatt atctggttcg atcatctgtc aactgacgtg atccatcagg
     8881 tggtggataa attcatcgtc gagttgcagg ttcagctgga tcagaaaggt gtttctctgg
     8941 aagtgagcca ggaagcgcgt aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg
     9001 cacgtccgat ggcgcgtgtc atccaggaca acctgaaaaa accgctcgcc aacgaactgc
     9061 tgtttggttc gctggtggac ggcggtcagg tgacggttgc gctggataaa gagaaaaatg
     9121 agctgactta cggattccag agtgcacaaa agcacaaggc ggaagcagcg cattaatctg
     9181 attgtcaggt aggttggtga agtccgtaat ctcgtcaggg gttacggact ttttatttat
     9241 ggggtggaac ggtgaaaacc ctatttttgg aggtgaaggt aagttgttga taattagtgc
     9301 tgctggaagg taaggataaa aaagggtgct gcaggagaat gggatggttt tgctttatta
     9361 acaacgggct aaacgtgtag tatttgagtt cactgccgta caggcagctt agaaatcgac
     9421 ggggtgcggt aaaacctttg cgaacgcgtt cactgccgta caggcagctt agaaattcac
     9481 aggtaacata ctccacccgc ccaccatgtt cactgccgta cagacagata aaatgcgaaa
     9541 aaaaagctcg cactttcgta cgagctcttc tttaaatatg gcggtgaggg ggggattcga
     9601 acccccgata cgttgccgta tacacacttt ccaggcgtgc tccttcagcc actcggacac
     9661 ctcaccaaat tgttttgcta ccaaacctca tgggtggcaa cggggcgcta ctatagggag
     9721 ttggagtaaa acggtcaaga agaattttaa tgataattat tgtttgctca tactgtaaac
     9781 aacttgtgca gtatatctac atcgagacaa gttatggact tatacttcca aagtacttca
     9841 tacatatcac aaaataaaaa ggccggttaa accgaccttt tactcgttct ttctcttcgc
     9901 ccatcaggcg gtaaaacaat cagcgactac ggaagacaat gcggcctttg ctcaggtcgt
     9961 acggggtcag ttcaacagtc actttgtcgc ccgtcaggat gcggatgtag tttttgcgca
    10021 ttttaccgga gatgtgtgca gtaaccacgt gaccgttttc taactctacg cggaacatgg
    10081 tattaggcaa cgtttcaaga acggtacctt gcatttcaat attgtcttct ttggccatct
    10141 aatcctctgg ggtatcacta ccgtaatttg aaccggcaag ataatgccga agttctgtaa
    10201 ataagtaaag atttgcgcgc taaatcgcaa caaacaggtt cggcacatta ctccgaaaac
    10261 acacggctaa gccgcaccaa aagcgcaacg tataagggag cggtgagata aacgatgggc
    10321 gttacctgac gcgaaaaatt ccttatcggc agcggggtaa tgagcgtaac caactctgcg
    10381 accgcaatta taacactctg gggagaaatg tgccgaaaac attcattctt gtggtgaaaa
    10441 caagcaccgt ggtacccaga aattattcgg caatcgtccg aggcgcattt gattgagata
    10501 attaaggtaa tcccggcggg gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag
    10561 gacctggcag tcgataagct taccgccatg accgataaat tcctcacaga ataccagaag
    10621 cgccgtttta gacgcatttt ccatccggct gaacatggac tcgccacaaa atagcgttcc
    10681 ctgggccacg ccgtacatac cgccgacaag ctcatcttca cgccagactt caatggagtg
    10741 ggcatgaccg agttcgtgaa ggcgatggta ggcttcgacc acgccacgcg tgatccaggt
    10801 tccttcttcg cgatcgctgg cacagccttc aatgacctga ccaaaagcgt aattcatcgt
    10861 gacacgatag ggcgagcgtt tatgaaatcg cttcatacta cggctgatat gcagtgattc
    10921 tggccatagc accgcgcggg gatcgggcga ccaccagagg atggggtcgt ctggagaaaa
    10981 ccacggaaaa ataccacgct ggtaagccat taacaggcgc gcagggctaa gatcgccccc
    11041 aagtgccagc aggccgttag gctcacgtaa tgcgccttcc ggggaaggga aggctattga
    11101 atggcgagaa agctgaacca agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca
    11161 ataatagctt acaaaccctg cttgaactgg taataacgcc cctgtctggc aagcagttct
    11221 gcgtgagtac cttgctcaat aatttgcccg ttgtccatca ctattatttg ttggaaacga
    11281 gagagtccgc gaagtcgatg ggtgaccatt aacaccgttt tctcacgcat catttttgca
    11341 agcaattcaa ggatctggct ttcggttgtg gcatctaagc cttcggtagg ttcatccagc
    11401 aacaccagtg gcgcatcatg taacagcgta cgggcgatag ccagacggcg cagttcacca
    11461 ccggagagct ggcgtccgcc ttcacctaac caactgttga gacctgcatc ctcgagcagc
    11521 ttctccagac caacgcgacg caagatctcc gacagagcct catcactact gccaggcgag
    11581 gcgagtaaca gattatcacg cagtgtggcg ctaaacagat gcactcgctg aggaacaacg
    11641 ctgatggtct gtcgtagagc cgcttcattc aggctggcta tggggctatc gttaagcaaa
    11701 atctcgccct gttgcgggtc ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg
    11761 catccggttc gcccgagaat cgctatatgt tccccggcgt ttacctgaag agaaatccct
    11821 ttaagtgcct gttgggattg ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa
    11881 acgcgatcgg caacacgagt ttgggtatca gggaaggtga cctccggttt ttgatccgtt
    11941 aagtcagaga tacgtacggc agaggcaatg acttgcccca gatgctgaaa tgcacccgtt
    12001 actggtgcca gtgcttcaaa cgcggctaac gcacaaaaga caaacagggc aattaatgcg
    12061 ccgggttgag cattgccgcc aacgccgcca gacgccatcc acagcatcag gatcaccgct
    12121 aacgcgccaa tgagcagcat tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt
    12181 tgcgcttcca gccattgaat ttctgtattc tctagttgcg tgcgataacg atcgctggca
    12241 ccaaaaatgg tcagctcagc ttgcccttgc agccaggccg tcagttgttg gcgatactgt
    12301 ccgcgaagat gagtcagatt ttgcccggtg ctttttcccg cacgataaaa cagcggtggc
    12361 atcaggaaaa gcgtcagtaa cataacgctg cccagcgtaa aggcgagggt gaaatcaagg
    12421 aaacttaacc cgattgtcac caccataatc accacaaaag cgcccaccag cggcgagata
    12481 acgcgcaggt aaagatgatc gagcgtatca acatccgcca ccacgcggtt gagcaattcg
    12541 ccctgacgat agcgcgccag tccggcaggg gagaggggca acaatttgct gaaggtgtaa
    12601 atgcgcagat gctgcaacac gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa
    12661 cgcccggcag tacgggtgat tgcggcacca cgcacgcccg cagcgggtag catatagttg
    12721 aagctgtaca gtccggcaac ccccgcaacc gctgaggccg agaggaacca gccggaaagt
    12781 gtcaacagac cgatactggc gagcagcgtc acaattgcca gcacaatacc aagacttaac
    12841 atccatttat gacgtttata cagtgccaga tagggtagca aagcgcgcat ttaaatctcc
    12901 tcctgacgat gggccagtaa tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa
    12961 cgtccttgct caataatccg gccatcctgc ataacccaaa tgacatccca gtcagcaaga
    13021 tcttctaact ggtgggtgac cattaacgtt gtctggcgca gagaggcggc attcagcgcc
    13081 tccattacgc gctgttcact gtgagcatca aggctggcag cgggttcatc caacagtaat
    13141 agcgaacagg gatttagtaa cgcacgggcc accgccacgc gctgcgcctg ccccacggaa
    13201 aggcgggcag cctggtcgcc aacaggtgta tcaacgcctt gtggcaggag cggtagaaac
    13261 tcgctgaccc aggcgttatc cagcgctgct tgtaattctt gttcgctggc atcaggtcgc
    13321 gccagtagta cgttatcccg caattttgct gccggtaatt gtgggttttg cccaacccag
    13381 gagagatgtt tacgccatga ttctggtgat aaatcacgta attctatccc gttgattcgt
    13441 aatgatccct gatatgagag aaaaccagaa agcgcgttca gcagtgagct tttacctgaa
    13501 ccgctgcggc caaccaacac tgcacgttgg cctgctggca aagtaaagtt cagcggtccg
    13561 gccagcgttt taccttccgg cgacgtgata aacagatcct cggcctcaat ggtcagcgga
    13621 tcggtcaatg ctaattccgc ctcaccgcgc tgcggatggg cgagcggggt ttccataaac
    13681 gttttcagac tgtcagctgc accaacagcc tgggctttag catgataaaa cgtaccgaga
    13741 tcgcgtaatg gctggaaaaa ctctggcgca aggatcaggg ccagaaaacc tgcagccagc
    13801 gtcaccccag tatcgtagtg accaaaatcc agctcgccga gataggaaaa accaaagtag
    13861 accgccacca gagcaattga cagcgaggta aaaaattcga gaatgccgga ggataaaaac
    13921 gccagtcgta gcacttccat tgtccgttgg cggaaatctt ccgaagcaga acgaatactt
    13981 tcaatttcag cttcaccacg accaaaaata cgcaatgttt ccatgccgcg caggcgatcg
    14041 aggaaatgcc cacttaagcg agcaagagcg agaaagttac gccggttagc atcggcagcc
    14101 cccattccaa ccagcgccat aaacaacgga attagcggtg ctgtgcccag cagaatgagc
    14161 gccgcagccc agttagaggg gaagatcgcc accacaatca gcaacggcac cgacactgcc
    14221 agcgccattt gcggcagata gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt
    14281 accagcgtcg cccagctccc cgcaggttta ccctgaatcc acgctggccc tgcttgttgc
    14341 agacggtcga gaacctgacg gcggatggca aagcggatat gctgcccggc gtgataaccc
    14401 acccgttcgc gtaaccagac cacccatgcg cgcaatacaa aggtcagaac cagtaacgta
    14461 aagggaagca gcagggcttc acggggaata ttctccataa tcatatgttg cagaattcgc
    14521 gccatgaacc aggcctgggc aatgatcaat atgccgctta caaagcccag cagacgagaa
    14581 atattcagcc aacgttggga gatgacgctt tgctgtttta accagcgggt taactctttt
    14641 tgacgagatt tattcattgc acgcttagca ggtgagttat cagaattatt tgcagagcaa
    14701 tgttacaacg gggaaaaaat aaaggcgacc catagtcgca aggtgtcgcc ttctttactt
    14761 ttgttactga tttgtaaaat tattttgcgt cagctaaacc atcgaggtag cgttccgcat
    14821 caagtgctgc catgcagcca gtaccggccg aagtaatggc ctggcgataa atgtgatcca
    14881 tcacgtcgcc tgcggcaaag acgccaggaa tgctggtctg ggtggcatta ccatgaatac
    14941 ccgactgtac tttgatgtag ccgttttcca gttccagctg cccttcgaaa atcgcagtat
    15001 tcgggctgtg accgatagca acaaacagac cggcaacgtc gagtgactcg atgttatcgc
    15061 tgttttgcgt atcgcgcaga cgaacgccag tgacacccat ttgatcgccg gtcacttctt
    15121 ccagcgtacg gttggtgtgc agaatgatgt tgccgttctc cactttatcc atcagacgct
    15181 taataaggat tttttccgcg cggaaaccgt cacggcggtg aatcagatgc acttccgaag
    15241 cgatgttaga cagatacagc gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa
    15301 ctttctggtt gcgatagaag aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt
    15361 taaaggcttc ttcagagggc aggccgagat agcgtgcaga agctccggtg gcaataatca
    15421 gcgcgtcgca agtgtattcg ccgttatcgc cattcagacg gaacggacgg ttttgcagat
    15481 ccaccttgtt gatatgatca aaaatgatct cagtttcaaa cttggtggca tgttcgtgca
    15541 tgcgcgccat taataacgga ccggtcagat cgtttggatc accaggccag ttttccactt
    15601 ccgtggtggt ggtcagttgg ccgccttttt ccatgccggt aatcagcaca ggttgcaggt
    15661 tggcgcgcgc cgcgtagaca gcagcggtgt atcccgccgg gcctgaaccc aggataagca
    15721 gtttactgtg tttggtcgtg cccatgagat ccccatagtt gttggcagac aatgggcagg
    15781 attgtaggga atttacagac gtaaaaaaag agtatgacga ttttgttaac aatttgtgca
    15841 atcggcagca tcgataagca ggtcaaattc tcccgtcatt atcacctctg ctacttaaat
    15901 ttcccgcttt ataagccgat taaatgatga ataaacgccc ctgttaatga atatctggca
    15961 tgttgtacta aaaatcgatg ttttgctttg acaatcccct ggtgttttgc gaaaacattc
    16021 gaggaagaaa aaaacagtat tcttatatgc gcataaccat gtatgtaaat accatgttta
    16081 ccgtgctagt gaaatctacg tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc
    16141 cacaaggcaa cggtcttctc accgtagacc caggcattgc gcgccgtgaa tcttcatgat
    16201 ttcggtctat cgtgacgggt agcgactctg aacagtgatg tttcagggtc agacaggagt
    16261 agggaaggaa tacagagaga caataataat ggtagatagc aagaagcgcc ctggcaaaga
    16321 tctcgaccgt atcgatcgta acattcttaa tgagttgcaa aaggatgggc gtatttctaa
    16381 cgtcgagctt tctaaacgtg tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg
    16441 gctggaaaga caagggttta ttcagggcta tacggcgctg cttaaccccc attatctgga
    16501 tgcatcactt ctggtattcg ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga
    16561 acaattcaat accgctgtac aaaaacttga agaaattcag gagtgtcatt tagtatctgg
    16621 tgatttcgac tacctgttga aaacacgcgt gccggatatg tcagcttacc gtaagttgct
    16681 gggggaaacc ctgctgcgtc tgcctggcgt caatgacaca cggacatacg ttgttatgga
    16741 agaagtcaag cagagtaatc gtctggttat taagacgcgc taacacggaa caggtgcaaa
    16801 atcggcgtat tttgattaca ctcctgttaa tccatacagc aacagtactg gggtaacctg
    16861 gtactgttgt ccgttttagc atcgggcagg aaaagcctgt aacctggaga gcctttcttg
    16921 agccaggaat acactgaaga caaagaagtc acattgacaa agttaagtag cggccgccgc
    16981 cttctggagg cattgctgat ccttattgtc ctgtttgccg tctggttgat ggctgcctta
    17041 ctaagcttta acccttcgga ccccagctgg tcgcaaacgg cctggcatga acctatccat
    17101 aatttaggtg ggatgcccgg tgcgtggttg gcagatacgc tgttctttat ttttggcgtg
    17161 atggcttaca ccattcccgt cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag
    17221 tccagcgacg aatacattga ttattttgcc gtttcgctac gcatcattgg cgttttggcg
    17281 ctcatcctta cctcctgtgg tctggcggca atcaacgctg acgatatctg gtattttgcc
    17341 tccggtggcg tcattggcag cttactaagc actacgctac aaccactgct acacagtagc
    17401 gggggaacta ttgcgctgct ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg
    17461 tcatgggtga ccattgctga aaaactcggc ggctggattt taaacattct caccttcgcc
    17521 agtaatcgta cccgtcgcga tgatacctgg gtcgatgaag atgagtatga agacgacgaa
    17581 gagtatgaag atgaaaatca cggcaaacag catgaatcac gccgtgcccg tattcttcgc
    17641 ggcgcgctag cgcgtcgtaa acggttggcg gaaaaattca ttaatccgat ggggcggcaa
    17701 acagacgctg cgttgttctc cggtaagcgg atggatgatg acgaagagat tacctacact
    17761 gcacgcggtg tggctgccga cccggacgac gtcctatttt cgggcaatcg tgcaacgcag
    17821 ccagaatatg acgaatacga tccattatta aacagtgcgc caattaccga acctgtcgct
    17881 gtagcagctg ctgctaccac ggcgacacaa agctgggctg cgccggttga acctgtgact
    17941 cagacgccgc ctgttgcctc tgttgatgtt ccaccttcgc aacctacagt agcctggcag
    18001 cctgtaccgg gtccacaaac gggagagccg gttattgctc ctgcaccgga aggttaccca
    18061 cagcagtcac aatatgcgca gcctgcagtg caatataatg agccgctgca acaaccagta
    18121 cagccgcagc agccgtatta tgcacctgca gctgaacaac ctgcgcaaca gccgtattat
    18181 gcccctgcgc cagaacaacc ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc
    18241 acttttgctc cacagtctac ataccagact gagcaaactt atcagcagcc agccgctcag
    18301 gagccgttgt accaacagcc gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc
    18361 gttgtagaag agacaaaacc cgcgcgtccg ccgctttact attttgaaga agtggaagag
    18421 aagcgagccc gtgaacgtga acaacttgcg gcctggtatc aaccgattcc agaaccggtt
    18481 aaagaaccag aaccgatcaa atcttcgctg aaagcacctt ctgttgcagc agtacctcca
    18541 gtagaagccg ctgccgctgt ttctccgctg gcatctggcg tgaaaaaagc gacactggcg
    18601 acgggggctg ccgcaaccgt tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg
    18661 cgtccgcagg tcaaagaggg gattggcccg cagttgccac gaccgaaacg tatccgcgtg
    18721 ccaactcgtc gtgaattggc gtcttacggt attaagctgc cctcacagcg tgcggcggaa
    18781 gaaaaagccc gtgaagccca gcgcaatcag tatgattctg gcgatcagta taacgatgat
    18841 gaaatcgatg cgatgcagca ggatgaactg gcacgtcagt tcgcccagac acagcagcaa
    18901 cgctatggcg aacagtatca acatgatgtg cccgtaaacg cagaagatgc agatgctgcg
    18961 gcagaggctg aactggctcg tcagtttgcg caaactcaac aacaacgtta ttccggcgaa
    19021 caaccggctg gggcgaatcc gttctcgctg gatgattttg aattttcgcc aatgaaagcg
    19081 ttgctggatg atggtccaca cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag
    19141 ccgcaacaac cggttgcacc acagcagcag tatcagcagc cacaacagcc ggttgcgcca
    19201 cagcagcagt atcagcagcc acaacagcag gttgcgccgc agccacaata tcagcagccg
    19261 caacaaccgg ttgcgccaca gcagcaatat cagcagccgc aacaaccagt tgcgccgcag
    19321 ccgcagtatc aacagccgca acaaccggtt gcaccgcagc cgcagtatca gcagccacaa
    19381 cagccagttg cgccacagcc gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc
    19441 gacagccgtc cgttgcacaa accgacgacg ccactgcctt ctctggattt gctgacaccg
    19501 ccgccgagcg aagtggagcc ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg
    19561 gaagcgcgtc tggctgattt ccgtattaaa gccgatgtcg ttaattactc tccggggcca
    19621 gttatcactc gctttgaact taacctggct cctggcgtaa aagcggcgcg catttctaac
    19681 ttgtcacggg accttgcccg ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt
    19741 cctggcaaac cctatgtagg tctggagtta ccaaataaaa aacgacaaac cgtttatctg
    19801 cgcgaagttt tggataacgc caaattccgc gataatccgt cgccattaac cgtggtgctg
    19861 ggtaaagata tcgccggtga gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg
    19921 gttgcgggga ctaccggttc cggtaaatct gtcggtgtga acgcgatgat cctgagcatg
    19981 ctttataaag cacagccaga agatgtgcgt ttcatcatga tcgacccgaa aatgctggag
    20041 ctttcggttt atgaaggcat tccgcacctg ttaacggaag tcgttactga tatgaaagat
    20101 gccgccaacg cgctgcgctg gtgtgttaac gagatggagc gtcggtataa actgatgtct
    20161 gcgctgggtg tgcgtaatct ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg
    20221 atgcgtccga ttccagaccc gtactggaag ccgggtgaca gtatggatgc ccagcatccg
    20281 gtgctgaaaa aagaaccata cattgtcgtg ttggttgacg aatttgccga cctgatgatg
    20341 acggtaggta aaaaagtgga agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg
    20401 ggtatccacc tcgtactggc aactcagcgt ccatcggttg atgttattac tggtctgatt
    20461 aaagcgaata ttccgacccg tatcgccttt accgtatcca gtaagattga ctcacgtacc
    20521 attcttgatc aggctggcgc ggaatcactg ctgggtatgg gggatatgct ctactctggg
    20581 ccgaactcca cgttgccggt acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat
    20641 gccgtggtgc aggactggaa agcgcgtggt cgcccacagt atgttgatgg catcacctcc
    20701 gacagcgaaa gcgaaggtgg tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg
    20761 ttcgatcagg cggtgcagtt tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag
    20821 cgtcagttcc gcattggtta taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag
    20881 gggattgtca gcgaacaggg gcacaacggt aatcgtgaag tgctggcccc accgccgttt
    20941 gactaattaa tgcattgccg gataaggcgc ggtagcgtcg catccggcac tctatcaact
    21001 gaaaattcag tattttcttc tttcctcaag ctgattatta gcctggaata gagagtagag
    21061 ggaactcccg atcgggagtg acgtaatttg aggaataatg atgaaaaaaa ttgctatcac
    21121 ctgtgcatta ctctcaagct tagtagcaag cagcgtttgg gctgatgccg caagcgatct
    21181 gaaaagccgc ctggataaag tcagcagctt ccacgccagc ttcacacaaa aagtgactga
    21241 cggtagcggc gcggcggtgc aggaaggtca gggcgatctg tgggtgaaac gtccaaactt
    21301 attcaactgg catatgacac aacctgatga aagcattctg gtttctgacg gtaaaacact
    21361 gtggttctat aacccgttcg ttgagcaagc tacggcaacc tggctgaaag atgccaccgg
    21421 taatacgccg tttatgctga ttgcccgcaa ccagtccagc gactggcagc agtacaatat
    21481 caaacagaat ggcgatgact ttgtcctgac gccgaaagcc agcaatggca atctgaagca
    21541 gttcaccatt aacgtgggac gtgatggcac aatccatcag tttagcgcgg tggagcagga
    21601 cgatcagcgc agcagttatc agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa
    21661 atttaccttc accccgccgc aaggcgtcac ggtagatgat caacgtaagt agaggcacct
    21721 gagtgagcaa tctgtcgctc gatttttcgg ataatacttt tcaacctctg gccgcgcgta
    21781 tgcggccaga aaatttagca cagtatatcg gtcagcaaca tttgctggct gcggggaagc
    21841 cgttgccgcg cgctatcgaa gccgggcatt tgcattctat gatcctctgg gggccaccgg
    21901 gtaccggcaa aacaaccctc gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac
    21961 gtatttctgc cgtcacctct ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc
    22021 aaaaccgcaa tgcaggtcgc cgcactattc tttttgttga cgaagttcac cgtttcaaca
    22081 aaagccagca ggatgcattt ctgccacata ttgaagacgg caccatcact tttattggcg
    22141 caaccactga aaacccgtcg tttgagctta attcggcact gctttcccgt gcccgtgttt
    22201 atctgttgaa atccctgagt acagaggata ttgagcaagt actaactcag gcgatggaag
    22261 acaaaacccg tggctatggt ggtcaggata ttgttctgcc agatgataca cgacgtgcca
    22321 ttgcagaact ggtgaatggc gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg
    22381 atatggccga agtctatgat agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa
    22441 tcgccggtga acgtagcgcc cgctttgata acaaaggcga tcgcttttac gatctaattt
    22501 ccgcactgca taagtcggta cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc
    22561 gaattattac cgctggtggc gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt
    22621 ctgaagacgt cggtaatgcc gatccacggg cgatgcaggt ggcaattgcg gcctgggatt
    22681 gctttactcg cgttggcccg gcggaaggtg aacgcgccat tgctcaggcg attgtttacc
    22741 tggcctgcgc gccaaaaagc aacgctgtct acactgcgtt taaagccgcg ctggccgatg
    22801 ctcgcgaacg cccggattat gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa
    22861 tgaaggaaat gggctacggg caggaatatc gttatgctca tgatgaagca aacgcttatg
    22921 ctgccggtga ggtttacttc ccgccggaaa tagcacaaac acgctattat ttcccgacaa
    22981 acaggggcct tgaaggcaag attggtgaaa agctcgcctg gctggctgaa caggatcaaa
    23041 atagccccat aaaacgctac cgttaatgtt atcgttgcgg taatgttgtt actgtatccc
    23101 tgtggtcgca ggctgtggcc acatctccca tttaattcga taagcacagg ataagcatgc
    23161 tcgatcccaa tctgctgcgt aatgagccag acgcagtcgc tgaaaaactg gcacgccggg
    23221 gctttaagct ggatgtagat aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg
    23281 tcaaaacgga aaacctgcaa gcggagcgta actcccgatc gaaatccatt ggccaggcga
    23341 aagcgcgcgg ggaagatatc gagcctttac gtctggaagt gaacaaactg ggcgaagagc
    23401 tggatgcagc aaaagccgag ctggatgctt tacaggctga aattcgcgat atcgcgctga
    23461 ccatccctaa cctgcctgca gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg
    23521 aagtcagccg ctggggtacc ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc
    23581 tgggtgaaat gcactctggc ctcgactttg cagctgcagt taagctgact ggttcccgct
    23641 ttgtggtaat gaaagggcag attgctcgca tgcaccgcgc actgtcgcag tttatgctgg
    23701 atctgcatac cgaacagcat ggctacagtg agaactatgt tccgtacctg gttaaccagg
    23761 acacgctgta cggtacgggt cagctgccga aatttgctgg cgatctgttc catactcgtc
    23821 cgctggaaga agaagcagac accagtaact atgcgctgat cccaacggca gaagttccgc
    23881 tgactaacct ggtgcgcggt gaaatcatcg atgaagatga tctgccaatt aagatgaccg
    23941 cccacacccc atgcttccgt tctgaagccg gttcatatgg tcgtgacacc cgtggtctga
    24001 tccgtatgca ccagttcgac aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa
    24061 tggcggcgct ggaagagatg actggtcatg cagaaaaagt cctgcagttg ctgggcctgc
    24121 cgtaccgtaa aatcatcctt tgcactggcg acatgggctt tggcgcttgc aaaacttacg
    24181 acctggaagt atggatcccg gcacagaaca cctaccgtga gatctcttcc tgctccaacg
    24241 tttgggattt ccaggcacgt cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa
    24301 cccgtctggt tcataccctg aacggttctg gtctggctgt tggtcgtacg ctggttgcag
    24361 taatggaaaa ctatcagcag gctgatggtc gtattgaagt accagaagtt ctgcgtccgt
    24421 atatgaacgg actggaatat attggctaat acccaatttt tctgaatcta aaaagcgcct
    24481 ttagggcgct ttttttgtct ccctttgata ccgaacaata attactcctc acttacacgt
    24541 aatactactt tcgagtgaaa atctacctat ctctttgatt ttcaaattat tctatgtata
    24601 caagcctata tagcgagctg ctatagaaat aattacacaa tacggtttgt tactggaatc
    24661 aatcgtgatc aagcttgagt gagccattat gaaaacgaaa atccctgatg cggtattggc
    24721 tgctgaggag agtcgccgtg gtttggtaaa aacgacagcg atcggcggcc tggcaatggc
    24781 cagcagcgca ttaacattac cttttagtcg gattgcgcac gctgtcgata gcgccattcc
    24841 aacaaaatca gacgaaaagg ttatctggag cgcctgtaca gttaactgtg gtagtcgctg
    24901 cccgctacgt atgcacgtcg tggacggtga aatcaaatat gtcgaaacga caataccggc
    24961 gatgacaatt acgacggcct acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt
    25021 caccgtgtct acaatccgga ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt
    25081 gaaggcaaat tcgagcgcat tagctgggaa gaagcctacg acatcatcgc gaccaatatg
    25141 cagcgcctga tcaaagagta cggcaacgag tctatctatc tgaactatgg caccggtacg
    25201 ctgggcggca ccatgacccg ctcctggccg ccgggaaata ccctagtcgc gcgcctgatg
    25261 aactgctgcg gcggttatct taaccattac ggtgactact cttccgcgca aattgcggaa
    25321 ggtttgaacc atacctacgg cggctgggca gatggtaaca gcccgtcgga tatcgaaaac
    25381 agtaaattgg tagtgctgtt tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg
    25441 gtgacttact atcttgaaca ggcacgccag aaatctaatg ctcgcatgat catcatcgat
    25501 ccgcgctata ccgacaccgg tgccgggcgc gaagatgagt ggatccctat tcgtccagga
    25561 acagatgccg cactggttaa cggtctggcg tacgtcatga tcactaaaaa cctggtggat
    25621 caggcattcc tcgataaata ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg
    25681 ccgaaaaatg gtcattataa agcttatatt ctgggtgaag ggccagatgg cgtggctaaa
    25741 acaccggaat gggcctcgca aatcaccggt gttccggcag acaaaatcat caaactggct
    25801 cgtgaaatcg gcagtaccaa accggcgttt atcagccagg gatggggccc gcagcgtcac
    25861 gctaacggtg aaatcgcaac ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt
    25921 ggtattaatg gaggggacag cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt
    25981 atgccgacct tggaaaaccc gatccagacc agcatttcga tgtttatgtg gaccgatgcc
    26041 attgaacgtg gcccggaaat gacggcgctg cgtgatggtg tgcgcgggaa agataagctg
    26101 gatgtgccga tcaaaatgat ctggaactat gccggtaact gcctgattaa ccagcattct
    26161 gaaatcaacc gtacccatga aatcctccag gatgataaga agtgcgagct gattgtggtt
    26221 atcgactgcc acatgacctc atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc
    26281 gcttccgaac agatggactt tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt
    26341 ttcaacgatc aggtgattaa accgcgcttt gaatgtaaga ccatctatga aatgaccagc
    26401 gaactggcaa aacgtcttgg cgttgagcaa cagtttactg aaggccgtac ccaggaagag
    26461 tggatgcggc atctgtatgc ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa
    26521 gagttccgca agcaggggat ctttaaaaag cgcgacccac aagggcatca cgttgcttat
    26581 aaagccttcc gtgaagatcc gcaggcaaac ccactgacta cgccatcggg caaaattgag
    26641 atttattcgc aggcgctggc tgacattgcc gctacctggg aattgccaga aggcgatgtg
    26701 atcgatccac tgccgatcta cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa
    26761 cagtatccgc tgcagcttac aggtttccac tataaatctc gcgttcactc aacttacggc
    26821 aacgttgatg tgctgaaagc ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc
    26881 caaaaacgcg gtatccacaa cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt
    26941 catattgagg cgaaagtgac gccacgaatg atgccgggcg tggtcgcact gggtgaaggc
    27001 gcctggtatg acccggatgc aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc
    27061 actcaacgtc cgtctcctct cgctaagggg aatccgtcac atacaaacct tgttcaggtt
    27121 gaaaaggtgt aaggagtaac cgatgacaac ccagtatgga ttttttattg attccagccg
    27181 ttgcaccggt tgcaaaacct gcgagctggc ttgtaaagac tacaaagatt tgacgccaga
    27241 agtcagtttc cgccgcattt atgaatatgc tggcggcgac tggcaggaag ataacggtgt
    27301 ctggcaccag aacgtgtttg cctactatct gtcgatttcg tgcaaccact gcgaagatcc
    27361 ggcttgtact aaagtctgcc cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt
    27421 ggtcgatgaa gatgtatgca ttggctgccg ttactgtcat atggcttgcc cgtatggcgc
    27481 accgcaatat aacgaaacga aaggccatat gaccaaatgc gatggttgtt atgaccgtgt
    27541 tgcggagggt aaaaagccga tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg
    27601 ccctatcgac gagctgcgta aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg
    27661 agctcacttt accaaaccga atattgtgat caaacccaat gccaatagcc gcccgaccgg
    27721 ggataccact ggctatctgg caaacccgaa ggaggtgtga gatgggaagt ggatggcatg
    27781 aatggccgct gatgatcttc acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc
    27841 tggctttggc gctgctcaaa ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct
    27901 gcatgtttgg cttatgggta ctgatgggca ttggctttat cgcctctatg ctccatcttg
    27961 gttcaccgat gcgcgccttt aactcgctca accgggtagg ggcttcagca ctcagtaacg
    28021 aaatcgccag cggttcgatc ttttttgctg taggcggcat cggctggttg ctggcaatgc
    28081 tgaaaaagct gtcaccggca ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg
    28141 tcgtctttgt ctggatgatg gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt
    28201 acagcatctg gacgccgatg ggctgggcga tgcgtctgct gccagccatt tctgtactgg
    28261 cactggtggt gagtggcgtg atgtcagtga tgcagggcac agagctggca actattcata
    28321 gttctgtgca gcaggccgca gcgctggtgc cggactatgg tgcgctgatg tcctggcgga
    28381 tcgtgctttt ggccgttgcc ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc
    28441 ctgcggttcc gctactatcc gtctcattca ttctgcttct ggcaggggaa ttaatcggtc
    28501 gcggcgtatt ctatggtttg catatgaccg tggggatggc cgtcgcaagc taatcagtaa
    28561 caaccggggt ttcggccccg gttctctttt atttctgctt cgttaacgtg tcataactgg
    28621 tcatcaaatt acgataatca ggaatatggt tagagaacaa cgtcgccagt ccttcaatat
    28681 cattacgcca gtcacggtgc agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac
    28741 cagcttgcga catgcgatcc cacgccgaat gccgggtaat ttcattaaag gtccctgatg
    28801 catcagtcac gacaaagaca tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca
    28861 cgcaaacttc ggttaccaca ccggcaataa ttaactgttt tttacctgtc gctttgactg
    28921 cttttacaaa atcttcgtta tcccaggcgt taatattgcc cgggcgagca atgtaaggtg
    28981 catcggggaa ttgtgctttc agttctagaa ctaatgggcc gttaggaccg gtttcaaaac
    29041 tggtggtaag aatagttggt aagttgaaat acttagccag gtcacccagc gccagcacgt
    29101 tatttttaaa cttatcgggt tcgatatccc gtacaaggga aagtaaacca gcctggtgat
    29161 caacaagcaa aacggcagca tcatttttat caagacgaac atacggtttg gtcatcctct
    29221 tctcctttcg agagtcagtg gtacgtctga aatcggtcag acgcgatgca ttgctctgaa
    29281 agcatagacg ggaaatatga gtttgctgtg accatgaaat ttttcgactg aacgaagcgt
    29341 atgaaatttg tgttagatca ataaaaacaa tcagatgaga ttaatcatct cttcagcatt
    29401 agcgttatga atgagtaata taaatcgata ataccgccgc tgcgatatgc gcgtattttc
    29461 acccattgac aatgtttttg gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg
    29521 ttttaattca tgtccacgta tacccggcct gtcatgcttt tgctgtctgg cctgcttttg
    29581 ttgactctgg cgatcgcggt gttaaataca ctcgtgccgc tttggctcgc ccaggaacac
    29641 atgtccacat ggcaggtagg cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt
    29701 acattgctga cagggtatgt cattaaacgc attggcttta accgcagcta ttatctgtcc
    29761 tccttcattt ttgctgctgg ctgtgccggc cttggcctga tgattggttt ctggagctgg
    29821 ttggcgtggc gttttgtcgc gggcgtcggc tgtgccatga tttgggtggt tgttgagagc
    29881 gcgctgatgt gcagtgggac gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg
    29941 gtttattacg tgggaacgtt tttaggccag ttactggtca gcaaagtctc aaccgagctg
    30001 atgagcgtat tgccgtgggt tacaggtttg acattggcag ggatcttacc gctgttgttt
    30061 acgcgtgtcc tgaatcagca ggctgaaaac catgattcga cgtcaattac ggcaatgcta
    30121 aaactccgtc aggcgcggct tggcgtgaat ggctgcatta tctcaggaat cgttctggga
    30181 tctctatatg gcctgatgcc tctgtacctc aatcacaaag gggtgagcaa tgccagtatt
    30241 ggtttctgga tggcggtact ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt
    30301 ctggcggata agtttggtcg actgctggtt ttgcgtgttc aggtctttgt cgtcattctc
    30361 ggcagtattg cgatgcttag ccaggcggcg atggccccag cgttattcat cctcggtgcc
    30421 gctggcttta cgctatatcc ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat
    30481 caactggtgg cgatgaacca agccttactg ttgagctata ccgtgggaag tctgcttggc
    30541 ccgtcattta gcgctatgct aatgcagaat ttctccgata atttattgtt tatcatgatc
    30601 gccagcgtat cgtttatcta tttgctgatg ctgcttcgca acgccggtca tacgccgaaa
    30661 cccgttgctc acgtgtaaat gaattcaagc aaagtgtgaa cttactgttt cacactctgc
    30721 ttttttgttt cttctatctg acttgctttt ttccaaattt tattcgttta aaaataaaat
    30781 gtgcagcagg ttataatttt gcatttcgct atttccgcac ttcttatttg ccgcgcataa
    30841 tccctcgttt tacagatgcc cctttaattt tggcgaagga tttgtctatg gctgggaatg
    30901 ttcagga
//
LOCUS       DAZSNO010000033        30666 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZSNO010000033 DAZSNO010000000
VERSION     DAZSNO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30666
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.033"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..588
                     /locus_tag="ACHWN2_001465"
     CDS             <1..588
                     /locus_tag="ACHWN2_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFR8931057.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="ACHWN2_001466"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="ACHWN2_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR8931058.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="ACHWN2_001467"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="ACHWN2_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR8931059.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="ACHWN2_001468"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="ACHWN2_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR8931060.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="ACHWN2_001469"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="ACHWN2_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR8931061.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="ACHWN2_001470"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="ACHWN2_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR8931062.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="ACHWN2_001471"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="ACHWN2_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR8931063.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="ACHWN2_001472"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="ACHWN2_001472"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR8931064.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="ACHWN2_001473"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="ACHWN2_001473"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR8931065.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="ACHWN2_001474"
     CDS             complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="ACHWN2_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR8931066.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="ACHWN2_001475"
     CDS             complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="ACHWN2_001475"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR8931067.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="ACHWN2_001476"
     CDS             complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="ACHWN2_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR8931068.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="ACHWN2_001477"
     CDS             complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="ACHWN2_001477"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR8931069.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="ACHWN2_001478"
     CDS             complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="ACHWN2_001478"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR8931070.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="ACHWN2_001479"
     CDS             complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="ACHWN2_001479"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR8931071.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18720..18820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="ACHWN2_001480"
     CDS             complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="ACHWN2_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR8931072.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19444..20217
                     /gene="nudC"
                     /locus_tag="ACHWN2_001481"
     CDS             19444..20217
                     /gene="nudC"
                     /locus_tag="ACHWN2_001481"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR8931073.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20257..21321
                     /gene="hemE"
                     /locus_tag="ACHWN2_001482"
     CDS             20257..21321
                     /gene="hemE"
                     /locus_tag="ACHWN2_001482"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR8931074.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21478..22149
                     /gene="nfi"
                     /locus_tag="ACHWN2_001483"
     CDS             21478..22149
                     /gene="nfi"
                     /locus_tag="ACHWN2_001483"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR8931075.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22192..22782
                     /locus_tag="ACHWN2_001484"
     CDS             22192..22782
                     /locus_tag="ACHWN2_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR8931076.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22969..23241
                     /gene="hupA"
                     /locus_tag="ACHWN2_001485"
     CDS             22969..23241
                     /gene="hupA"
                     /locus_tag="ACHWN2_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR8931077.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23254..23949
                     /locus_tag="ACHWN2_001486"
     CDS             23254..23949
                     /locus_tag="ACHWN2_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR8931078.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="ACHWN2_001487"
     CDS             complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="ACHWN2_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR8931079.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24614..25990
                     /gene="zraS"
                     /locus_tag="ACHWN2_001488"
     CDS             24614..25990
                     /gene="zraS"
                     /locus_tag="ACHWN2_001488"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR8931080.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25987..27312
                     /gene="zraR"
                     /locus_tag="ACHWN2_001489"
     CDS             25987..27312
                     /gene="zraR"
                     /locus_tag="ACHWN2_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR8931081.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27309..28598)
                     /gene="purD"
                     /locus_tag="ACHWN2_001490"
     CDS             complement(27309..28598)
                     /gene="purD"
                     /locus_tag="ACHWN2_001490"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR8931082.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28610..30199)
                     /gene="purH"
                     /locus_tag="ACHWN2_001491"
     CDS             complement(28610..30199)
                     /gene="purH"
                     /locus_tag="ACHWN2_001491"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR8931083.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7191 a         7982 c         8440 g         7053 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggg cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggc
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cattgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggcagg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgtgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgt ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccaa gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaatatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat agacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacattgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggca tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcaaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag gggggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatattctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    11941 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacacct tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgtaaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13141 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13201 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13261 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13321 cataccggct gcaaaccctg agcggttaac acggcagcaa ccgcttccgg tctgcgatcg
    13381 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13441 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13501 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13561 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13621 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    13681 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    13741 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    13801 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    13861 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    13921 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    13981 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14041 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14101 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14161 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14221 cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc
    14281 atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt
    14341 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag
    14401 gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac
    14461 tgcggctgcc cggtccggac tgacgtgcca gcaggcctac ttctaccgcc agacgaaatg
    14521 ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt
    14581 cggcccccat ttccagcgcc tgcgtggcat ggctgggaac gccgatgcca gcatcgacaa
    14641 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    14701 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    14761 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    14821 gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    14881 gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    14941 tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga
    15001 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15061 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15121 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15181 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15241 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15301 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15361 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15421 caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc
    15481 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac
    15541 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag
    15601 gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc
    15661 gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg
    15721 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt
    15781 caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg
    15841 tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag
    15901 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    15961 gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16021 cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat
    16081 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16141 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16201 tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca
    16261 cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg
    16321 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16381 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16441 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16501 catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16561 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat
    16621 ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    16681 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    16741 acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga
    16801 catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct
    16861 gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga
    16921 ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    16981 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17041 cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag
    17101 cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca
    17161 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17221 atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17281 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc
    17341 ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg
    17401 caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17461 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17521 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17581 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    17641 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    17701 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    17761 tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    17821 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    17881 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    17941 caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18001 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18061 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18121 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    18181 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18241 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18301 gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc
    18361 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18421 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18481 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18541 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18601 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag
    18661 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    18721 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    18781 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    18841 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18901 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    18961 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19021 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19081 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19141 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19201 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19261 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19321 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19381 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19441 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19501 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19561 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag
    19621 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19681 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19741 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg tagccattgc
    19801 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19861 tcgctcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19921 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19981 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20041 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20101 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20161 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20221 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20281 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20341 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20401 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20461 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20521 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20581 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20641 cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20701 tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa
    20761 aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc
    20821 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    20881 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20941 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21001 accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc
    21061 ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21121 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21181 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21241 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21301 tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag
    21361 gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc
    21421 cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg
    21481 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat
    21541 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    21601 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    21661 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    21721 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    21781 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta
    21841 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    21901 ctctccagca aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    21961 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    22021 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    22081 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    22141 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    22201 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    22261 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    22321 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    22381 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    22441 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg
    22501 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    22561 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatggg cgatgaagag
    22621 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    22681 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    22741 gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    22801 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    22861 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    22921 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    22981 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    23041 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    23101 gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc gtactggccg caacccgcag
    23161 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    23221 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    23281 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    23341 gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    23401 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    23461 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    23521 acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac
    23581 gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    23641 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    23701 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    23761 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    23821 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    23881 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    23941 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    24001 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    24061 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    24121 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    24181 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    24241 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    24301 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    24361 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    24421 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    24481 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    24541 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    24601 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    24661 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    24721 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24781 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24841 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24901 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24961 ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    25021 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    25081 tggaatgtac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    25141 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    25201 tattctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    25261 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    25321 ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    25381 taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact
    25441 ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga
    25501 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    25561 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    25621 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    25681 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25741 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    25801 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    25861 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25921 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25981 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    26041 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    26101 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    26161 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    26221 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    26281 ggggcgcagg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    26341 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    26401 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    26461 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    26521 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    26581 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    26641 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    26701 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    26761 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    26821 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26881 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26941 atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    27001 aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt
    27061 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    27121 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat
    27181 attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    27241 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa
    27301 ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag
    27361 caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg
    27421 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca
    27481 tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg
    27541 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    27601 ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc
    27661 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27721 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27781 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27841 cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag
    27901 taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc
    27961 tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    28021 tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc
    28081 gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct
    28141 ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    28201 accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg
    28261 gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg
    28321 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    28381 tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg
    28441 atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta
    28501 cccggagcag caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    28561 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    28621 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    28681 cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg
    28741 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28801 ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28861 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca
    28921 cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac
    28981 gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca
    29041 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc
    29101 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    29161 aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat
    29221 ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    29281 cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct
    29341 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    29401 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg
    29461 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    29521 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    29581 ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt
    29641 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    29701 gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29761 cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat
    29821 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg
    29881 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29941 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    30001 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    30061 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    30121 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    30181 ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct
    30241 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    30301 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    30361 ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    30421 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30481 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgccttca tcgacacggc
    30541 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca
    30601 gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg cgctaaagcg
    30661 cgtcgc
//
LOCUS       DAZSNO010000034        30296 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZSNO010000034 DAZSNO010000000
VERSION     DAZSNO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30296
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.034"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="ACHWN2_001492"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="ACHWN2_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HFR8931084.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYILIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="ACHWN2_001493"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="ACHWN2_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR8931085.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="ACHWN2_001494"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="ACHWN2_001494"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR8931086.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="ACHWN2_001495"
                     /pseudo
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="ACHWN2_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="ACHWN2_001496"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="ACHWN2_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR8931087.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="ACHWN2_001497"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="ACHWN2_001497"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR8931088.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /gene="wza"
                     /locus_tag="ACHWN2_001498"
     CDS             complement(6041..7180)
                     /gene="wza"
                     /locus_tag="ACHWN2_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR8931089.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7489..7638
                     /locus_tag="ACHWN2_001499"
     CDS             7489..7638
                     /locus_tag="ACHWN2_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931090.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7839..9422
                     /locus_tag="ACHWN2_001500"
     CDS             7839..9422
                     /locus_tag="ACHWN2_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR8931091.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="ACHWN2_001501"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="ACHWN2_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR8931092.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="ACHWN2_001502"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="ACHWN2_001502"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR8931093.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="ACHWN2_001503"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="ACHWN2_001503"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR8931094.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..13623
                     /locus_tag="ACHWN2_001504"
                     /pseudo
     CDS             13381..13623
                     /locus_tag="ACHWN2_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13622..14563
                     /gene="mdtA"
                     /locus_tag="ACHWN2_001505"
                     /pseudo
     CDS             13622..14563
                     /gene="mdtA"
                     /locus_tag="ACHWN2_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14563..17685
                     /gene="mdtB"
                     /locus_tag="ACHWN2_001506"
                     /pseudo
     CDS             14563..17685
                     /gene="mdtB"
                     /locus_tag="ACHWN2_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17686..20751
                     /gene="mdtC"
                     /locus_tag="ACHWN2_001507"
     CDS             17686..20751
                     /gene="mdtC"
                     /locus_tag="ACHWN2_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR8931095.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20752..22167
                     /locus_tag="ACHWN2_001508"
     CDS             20752..22167
                     /locus_tag="ACHWN2_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8931096.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22164..23567
                     /gene="baeS"
                     /locus_tag="ACHWN2_001509"
     CDS             22164..23567
                     /gene="baeS"
                     /locus_tag="ACHWN2_001509"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR8931097.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPSPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23564..24286
                     /gene="baeR"
                     /locus_tag="ACHWN2_001510"
     CDS             23564..24286
                     /gene="baeR"
                     /locus_tag="ACHWN2_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR8931098.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24477..24809
                     /locus_tag="ACHWN2_001511"
     CDS             24477..24809
                     /locus_tag="ACHWN2_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR8931099.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24956..26317
                     /gene="yegQ"
                     /locus_tag="ACHWN2_001512"
     CDS             24956..26317
                     /gene="yegQ"
                     /locus_tag="ACHWN2_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR8931100.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26659..26976)
                     /gene="yegR"
                     /locus_tag="ACHWN2_001513"
     CDS             complement(26659..26976)
                     /gene="yegR"
                     /locus_tag="ACHWN2_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR8931101.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27382..28281
                     /gene="yegS"
                     /locus_tag="ACHWN2_001514"
     CDS             27382..28281
                     /gene="yegS"
                     /locus_tag="ACHWN2_001514"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR8931102.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28363..29136)
                     /locus_tag="ACHWN2_001515"
     CDS             complement(28363..29136)
                     /locus_tag="ACHWN2_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR8931103.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="ACHWN2_001516"
     CDS             complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="ACHWN2_001516"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR8931104.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7012 a         8046 c         7828 g         7410 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 aaaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagcatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctagcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgggtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgtgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 attccaggaa ggccagcagg tcaaagcagg cgatttactg gcagaaattg accccagcca
    13681 gttcaaagtt gcattagcac aaacccaggg ccaactggca aaagataaag ccacgcttgc
    13741 taacgcccgc cgtgatctgg cgcgttatca gcaactggca aaaaccaatc tcgtctcccg
    13801 tcaggagctg gatgcccaac aggcgctggt cagtgaaacc gaaggcacca ttaaggctga
    13861 tgaagcaagc gtcgccagcg cgcagttgca actcgactgg agccgcatca ccgcaccagt
    13921 cgatggtcgc gttggtctca agcaagttga tgttggtaac caaatctcca gtggtgatac
    13981 caccggaatt gtggtgatca cccagacgca tcctatcgat ttagtcttta ccctgccgga
    14041 aagcgatatc gctactgtag tgcaggcgca aaaagccgga aaaccgctga tggtagaagc
    14101 ctgggatcgc accaattcga agaaattaag tgaaggcacg ctgttaagcc tcgataacca
    14161 aatcgatgcc acaaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc
    14221 gctgtttccc aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt
    14281 agtgatccca acagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa
    14341 tagcgaaaac aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt
    14401 ggtgatccgt gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct
    14461 gaccgaaggg gcgaaagtgg aagtggtgga agcccagagc gccaccactc cggaagagaa
    14521 agccaccagc cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg
    14581 agcagcacag gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg
    14641 atggtggcga tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg
    14701 ccggaagtgg actatccgac cattcaggtg gtcacgctct acccaggtgc cagcccggat
    14761 gtcatgacct ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg
    14821 aaacagatgt cgtcgcaaag ttccggcggt gcgtcagtta tcactttgca gttccagcta
    14881 acattaccgc tcaatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac
    14941 ttgttgccga gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg
    15001 ccgatcatga cgctcgccgt cacctcaacc gccatgccga tgacgcaagt ggaagatatg
    15061 gtggaaaccc gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt
    15121 tccggcggtc agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc
    15181 ggcctgacca gcgaaaccgt gcgcaccgcc attaccggcg ctaacgttaa ctcggcaaaa
    15241 ggtagcctcg acggcccttc ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc
    15301 gccgaagagt atcgccagct aatcatcgcc taccagaacg gcgcgccaat tcgtctgggc
    15361 gatgtcgcaa ctgtagagca aggtgcagaa aacagctggc tcggcgcgtg ggcgaacaaa
    15421 gaacaggcca ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc
    15481 gacagcattc ggcagatgct gccacagctc actgagagtc tgccgaaatc ggtgaaggtg
    15541 acagtgcttt ccgatcgcac caccaatatc cgcgcatccg tcgatgatac tcagtttgaa
    15601 ttgatgatgg ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg
    15661 gcgaccatca ttcccggtgt tgctgtaccg ctgtcgttaa tcggcacttt cgcggttatg
    15721 gtgtttctcg atttttcaat caataacctg acactgatgg ctttaactat cgccaccgga
    15781 ttcgtggtcg atgacgccat cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc
    15841 gaaaaaccgt tggcggcggc gctcaagggc gcaggtgaaa tcggctttac cattatctcg
    15901 ctgaccttct cactgattgc ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg
    15961 cgactgttcc gcgaatttgc tattaccctg gcggtagcga ttttgatctc agcggtggtg
    16021 tcgctgaccc tgacaccgat gatgtgcgcg cggatgctca gccaggagtc gttgcgtaaa
    16081 cagaacagct tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt
    16141 cgtggactgg cgaaagtgct gaatcatccg tggctgacct taagcgtggc actcagcacg
    16201 ctgctgctta gcgtgctgct gtgggtgttc attccgaaag gtttcttccc ggtacaggac
    16261 aacggcatta ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc
    16321 cagcgacaac gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc
    16381 tcatttgttg gcgttgatgg cactaacccg tcgctgaata gtgcacgttt acaaatcaac
    16441 ctcaaaccgt tggatgaacg tgatgatcgg gtgcaaaaag tcatcgcccg tctgcaaacg
    16501 gcggtagata aagtgccggg cgtcgatctc ttcctgcaac caacgcagga cctgaccatc
    16561 gatactcagg tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg
    16621 ctcagtacct gggtgccaca gttgatggaa aaactccagc aactgccaca gctttctgat
    16681 gtctccagcg actggcagga caaagggctg gtggcgtatg tcaatgttga tcgcgacagc
    16741 gccagccgtc tggggatcag catggcggac gtcgataacg ccctgtacaa cgcgtttggt
    16801 cagcgactga tttccactat ttatactcag gctaaccagt atcgcgtggt gctggagcac
    16861 aataccgaaa ataccccagg cctcgcggcg ctggatacca ttcgcctgac cagcagcgac
    16921 ggtggcgtgg tgccgttaag ctcaattgcc aaaattgagc agcgttttgc gccgctttcc
    16981 atcaaccatc tggatcagtt cccggtaaca accatctcct ttaacgtgcc ggataactat
    17041 tcgctgggcg atgcggtgca ggcgattatg gacaccgaaa agacgctgaa tctgccggtg
    17101 gatatcacca cgcaattcta gggcagcacc ctcgccttcc agtcggcgct gggcagcact
    17161 gtctggctga ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat tctgtacgag
    17221 agctttattc acccgatcac cattctctcg acgctaccca ccgcaggggt cggcgcgctg
    17281 ctggcattaa tgattgccgg tagcgaactg gatgtgattg cgattatcgg cattattttg
    17341 ctgatcggta ttgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag
    17401 cgcgagcaag gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt
    17461 ccgatcctga tgaccaccct ggcggctctg cttggcgcgc tgccgctgat gttgagtacc
    17521 ggggtcggcg cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc
    17581 agccaggtgc tgacgctgtt caccacgccg gtgatttatc tgctgttcga ccgcctggca
    17641 ttgtggacca aaagtcgctt tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc
    17701 ctattcattt accgcccggt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc
    17761 atactgggtt tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt
    17821 atggtcagcg cctcgctgcc cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg
    17881 ccgctggagc gctcacttgg gcgcattgcc ggggttagtg aaatgacctc cagcagttcg
    17941 ctcggcagca cgcgcattat tttgcagttt gattttgacc gggatatcaa cggcgcagcg
    18001 cgtgatgtgc aggcggcgat caacgctgca caaagtttgc tgcccagcgg gatgcccagc
    18061 cgcccgacct atcgcaaagc gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca
    18121 tccgatactt attcgcaggg tgaactgtac gatttcgcct cgacgcagct ggctccgacg
    18181 atttcgcaaa tcgacggtgt cggtgatgtc gatgtcggtg gcagctcgct gcccgccgtg
    18241 cgcgtcgggc tgaccccgca ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc
    18301 actgccatca gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac
    18361 cgctggcaga tccagaccaa cgatgaactg aaaaccgccg ctgaatatca gccgctgatt
    18421 attcactaca acaacggcgg cgcggttcgt ctgggcgatg tggcgacggt gactgactca
    18481 gtgcaggatg tgcgcaacgc cgggatgacc aacgccaaac cggctatttt actgatgatc
    18541 cgcaaactgc cggaagccaa tattatccag acggtagaca gcatccgggc aaaattacca
    18601 gagttgcagg agaccattcc ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc
    18661 accattcgcg cctcgctgga agaagtcgag caaacgctga ttatctcggt ggcgctggtg
    18721 attctggtgg tctttttatt cctgcgctcg ggtcgcgcca ctattattcc cgccgttgcg
    18781 gtgccggttt cgctgattgg tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat
    18841 aatctttcgc tgatggcgct caccattgct actggtttcg tggtggatga cgccattgtg
    18901 gtgctggaaa acattgcccg tcatctggaa gcggggatga aaccactaca ggccgcgctg
    18961 caaggtactc gcgaagtcgg ctttacggtg ctgtcgatga gtctgtcact ggtggcggtg
    19021 ttcctgccgc tgctgttgat gggcggattg ccgggccgac tgttacgcga gtttgccgtg
    19081 acgctttctg tcgccattgg tatttcgttg ctggtttccc tgacgctaac gccaatgatg
    19141 tgcggctgga tgctaaaagc cagcaagccg cgtgagcaaa agcgactgcg tggttttggc
    19201 cgcatgttgg tagccctgca acaaggctac ggcaagtcgc tgaaatgggt gctcaatcat
    19261 acccgtctgg tgggcgtggt gctgcttggc accattgcgc tgaatatctc gatcccgaaa
    19321 accttcttcc cggagcagga caccggcgta ctgatgggtg ggatacaggc ggatcagagt
    19381 atttcgtttc aggcgatgcg cggcaaattg caggatttca tgaaaattat ccgtgacgat
    19441 ccggcagtgg ataatgtcac cggctttacc ggcggttcac gagttaacag tgggatgatg
    19501 tttatcaccc tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt
    19561 ctgcgcgtga agctggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaagat
    19621 attcgcgttg gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac
    19681 ctggcggcac tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa
    19741 ctggcggacg tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac
    19801 cgcgacacca tggcacggct gggaatcgac gtacaggccg ccaacagtct gttaaataac
    19861 gccttcggtc agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg
    19921 atggaagtgg atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc
    19981 aataacgaag gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc
    20041 ccactatcgg tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg
    20101 accggaaaat cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt
    20161 gtgccttcga cggtgcgcgg cagttttgcc ggcacggcgc aggttttcca ggagacgatg
    20221 aactcgcagg tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc
    20281 ctttacgaga gttacgtaca tccgctgacg attctctcca ccctgccctc ggcgggcgtt
    20341 ggagcgctgt tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg
    20401 atcatgctat taatcggcat cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt
    20461 gaagcccaac ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg
    20521 cgttttcgcc cgattatgat gaccaccctg gcggcgctgt ttggcgcgct gccgctggta
    20581 ttgtcgggcg gtgacggctc ggagctgcgg caacccctgg agatcaccat tgtcggcggt
    20641 ctggtgatga gccagctcct gacgctgtat accacgccgg tggtgtatct ctttttcgac
    20701 cgtctgcggc tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagat
    20761 cttcccgaca gcacccgctg gcaattatgg attgtggctt tcggcttctt tatgcagtcg
    20821 ctggacacca ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt
    20881 ccgttgcata tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc
    20941 gccagcggct ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg
    21001 ctgtttactc tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg
    21061 gcacgcgcgt tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg
    21121 atgaaaatcg taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt
    21181 caggtcggtc cgctgctcgg tccggcgctc ggcggtctgc tggtagaata cgcgtcgtgg
    21241 cactggatct ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgatg
    21301 ttaatgccga actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg
    21361 gcggttggca tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg
    21421 ccgctggcga ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg
    21481 cacgccagaa ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt
    21541 tcgctgggcc tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg
    21601 acaccggttt tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg
    21661 atcccgatgg tgcttggcag catgggaatg aagcgaattg tggtacaggt ggtgaatcgc
    21721 tttggttatc gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg
    21781 tttatgacta ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa
    21841 gggatggtca actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg
    21901 gacaatctgg cgagcagcgg aaacagcctg ctgtcgatga ttatgcaatt gtcgatgagt
    21961 atcggcgtca ccatcgccgg gctgttgctg gggctttttg gttcgcagca tatcagcgtc
    22021 gacagcggca ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc
    22081 gcccttccgg cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt
    22141 tcgcggcgaa aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt
    22201 ttctggcgat tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta
    22261 tcagttttga gcgtggcttt attgattaca tcaaacacgg caatgaacag cgattacagc
    22321 tgttaagtga tgcgcttggc gagcagtatg cgcagcatgg taactggcgc ttcctgcgca
    22381 acaacgatcg ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata
    22441 aacccagccc gggtatgccg ccgcacggct ggcgtaccca gttctgggtg gtcgatcaaa
    22501 acaacaaagt actggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca
    22561 ttctggtcaa cggtgcggaa gtgggcgcgg tgattgcctc ccctgttgag cggctgaccc
    22621 gcaatactga tatcaatttc gataaacaac agcggcaaac cagctggctg atcgtcgccc
    22681 tggcaacgtt actcgcggcg ctcgccacct ttctgctggc gcgcggtttg ctggcaccgg
    22741 taaaacgact tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa
    22801 cgcccaccag tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag ctcgccagca
    22861 cgctggagaa aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc
    22921 gtacgccatt agcggtgctg cgcggtgaac tggaagccat tcaggatggc gtgcgcaaat
    22981 tcacgccgga gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg
    23041 acgatcttca tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg
    23101 tagatttgat cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc
    23161 gtggcctgaa actgcaattt tccctgccag acagtatcac cgtatttggc gatcgcgacc
    23221 gtttaatgca gttattcaat aacttactgg aaaacagcct gcgctacact gacagcggcg
    23281 gcagcctgaa aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca
    23341 gtgcgccagg tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg
    23401 aaggttcccg caaccgcgcc agcggcggtt ccgggctggg gctggcgatt tgcctgaaca
    23461 ttgttgaagc ccataatggc cgcattattg ccgcccattc gccttttggc ggggtaagca
    23521 ttacagtaga gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat
    23581 cgacgaaaac acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct
    23641 cattgattat ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt
    23701 actggcgtat gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg
    23761 caccgatggc ctgacattat gccgggaaat tcgtcgtttt tccgatgttc cgatcgtgat
    23821 ggtgacggca aaaatcgaag agatcgaccg cctgctgggg ctggagattg gcgccgatga
    23881 ctacatctgc aagccgtaca gcccacggga agtggtagcg cgtgtcaaaa ccattttgcg
    23941 ccgttgtaaa ccgcaacgcg aacttcagca acaggatgcc gaaagcccgt tgattatcga
    24001 cgaaggtcgt tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt
    24061 tcgtctgctt aaaacgctct ctcacgaacc aggaaaagtg ttctcccgcg agcaattgct
    24121 caatcatctt tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa
    24181 aaatctgcgc cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta
    24241 cggcgttggt taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatta
    24301 ctttgttagc cggagatggc gttcagaccc gatccggcaa cattattcca cgcatggtca
    24361 gcaaactgac attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatca
    24421 gtaaacagca cctttagatg ctgttttcga taagcaattt caatcaagga gtcattatgg
    24481 ctggttggtt tgaactcagt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg
    24541 gcaatggtga gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg
    24601 gcatcgcgtc ggtgcgtagc aacagcccgc aagaagagcg ctatgagaaa aaaacggcaa
    24661 gcaacggcaa attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc
    24721 agatgtacgc caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca
    24781 caagccagac ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc
    24841 ggcgctttac cagatccccc ctacagcgct acaatgcccg cccttaaagt gggggcctcc
    24901 cctaaccgct tcatcaggtg aagcggatct gacctgtcat cagaacgaga gaattatgtt
    24961 taaaccggaa ctcctttccc cggcgggaac gctgaaaaat atgcgttacg ctttcgctta
    25021 tggcgcagat gctgtttatg cgggccagcc gcgttactcc ctacgtgtgc gcaacaacga
    25081 attcaaccac gaaaatcttc agctcggcat caatgaagcc cacgcgctgg ggaaaaagtt
    25141 ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg aaaaccttta tccgtgacct
    25201 gaaaccggtg gtggaaatgg ggccggatgc gctgattatg tccgatccag ggctgattat
    25261 gctggtgcgt gagcacttcc ctgaaatgcc gatccacctc tcggtgcagg ctaacgccgt
    25321 gaactgggcg acggtgaaat tctggcagca aatgggactg acccgcgtga tcctctctcg
    25381 cgagctgtcg ctggaagaga ttgaagagat ccataatcag gtgccggata tggagatcga
    25441 gatcttcgtt cacggcgcac tgtgcatggc ctactccggt cgctgcctgc tctctggcta
    25501 tatcaacaag cgcgacccga accagggcac ctgcaccaac gcctgccgct gggagtacaa
    25561 cgtccaggaa gggaaagaag atgacgttgg caacatcgta cacaagtacg agccgattcc
    25621 ggtgcaaaat gttgagccga cgctgggtat cggcgcgcca accgacaaag tgtttatgat
    25681 cgaagaagcc cagcgtccgg gcgagtatat gaccgcgttt gaagatgagc acggcactta
    25741 catcatgaac tcgaaagatc tgcgcgccat cgcccatgta gaacgcctga ccaaaatggg
    25801 cgtgcattcg ctgaaaatcg aaggtcgtac caaatctttc tactattgcg cacgcaccgc
    25861 acaggtttac cgcaaagcta tcgatgacgc cgctgcggga aaaccgttcg ataccagcct
    25921 gctggaaact ctggaaggtc tggcgcatcg tggctatacc gaaggtttcc tgcgtcgtca
    25981 tactcacgac gattatcaga actacgaata cggttattca gtttctgacc gccagcagtt
    26041 tgttggtgag tttaccggtg agcgcaaggg ggacctcgca gcggtagcgg tgaaaaataa
    26101 attctccgtt ggcgacagtc ttgagctgat gacgccgcaa ggcaacatta actttaccct
    26161 tgagcacatg gaaaacgcca aaggcgaagc tatgccggtc gcaccaggcg atggttatac
    26221 tgtgtggctc ccggtcccgc aggatcttga gctaaattac gcgctgctaa tgcgtaattt
    26281 ctccggggaa accacgcgta acccccacgg taagtgatta atttcgatta tttttcccgg
    26341 atggaaaatt cttagaaacc gatcacatac agctgcattt attaaggtta tcatccgttt
    26401 cgctgaaaaa cataatccat aaaatgctag ctgtaccagg aaccacctcc ttagcctgtg
    26461 taatctccct tacacgggct tattttttgt acataagctg taaagaagga ttgatccctg
    26521 ataaccgtct tccattgacc acatcgatag aatccccctt catagcacaa tgcctttcac
    26581 gtaacggtat cgtgctcgcc caggttccgg ctacgcacag ccagaacgcg catgtttgac
    26641 gcttacaaaa aaatattctc actctccaca tttgaatgtc agacgagcga cgccatgtaa
    26701 tcctgcacct tctgtcttca ggtcaactat ctgcattttt ttgccctgag taacacagaa
    26761 atgggctgca tcatttttta ctatattttc tgcaccagat attctgcccc tggctaaaga
    26821 agcttcggct tcggtgtagt attggttatc gagtttacgc tgaatattac ttttatatgc
    26881 aagaccaaat ttaccgatac ttgtttcatc attatgcaca gcacaaccag atataagaaa
    26941 aatactaatt aatgatatag cagctatctt tttcatctca ccttccccca ttaaatacca
    27001 acgacactct ctagtgttta aatataataa tggcatgatt attataattg aataggatta
    27061 taataaatgt tctgtacaac atttcctaca taagtaggaa ttacggacat tgaggccctt
    27121 cagggtaact ccatgggggc tttaatatat tatattgaag atgccactgt ttagttgaat
    27181 attaggtata tgctcttttt tgaaatttat cggtggcagc cgttagtatt cgctgtcccc
    27241 attgcaagct cctggtggta accactgaat cctccatact tgaactgact ttttatcctc
    27301 cgactttcat cctgttctga ctccaccttt tgttttctgc tctacactat ctacagacca
    27361 atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat tcttaatggt
    27421 aaaagtactg acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg
    27481 acgatccatg tgcgggtcac ctgggagaaa ggtgatgccg cacgatttgt agaggaggcc
    27541 cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat taatgaagtt
    27601 tctacggcgt tgattcagtg tgagggggat gacatacccg cgctgggaat tttgccatta
    27661 ggaaccgcca atgattttgc caccagtgta gggattcctg aggcactgga taaggcgctg
    27721 aaactggcaa ttgccggtaa tgccattgcg atagatatgg cgcaggtcaa caaacaaacc
    27781 tgttttatta atatggcgac aggcggattt gggacgcgta ttaccacaga aacgccggaa
    27841 aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat gcgcatggat
    27901 actctgcaac cggaccgttg tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc
    27961 ctggtcattg gtattggtaa cgggcgtcag gccggtggcg gtcaacaatt gtgcccgaac
    28021 gcgttaatta acgatggctt gctgcaactg cgcattttta ccggcgatga aattcttccg
    28081 gctctcgtat caaccttaaa atctgacgaa gataacccga atattatcga aggcgcttcg
    28141 tcgtggtttg atatacaagc cccacacgaa atcactttta atcttgatgg cgaaccgttg
    28201 agtgggcaaa acttccatat tgaaatactt ccggcggcgt tgcgttgtcg attaccacca
    28261 gattgtccat tattgcgtta atcaaactat tctctcttga accagggaga atccgtaatg
    28321 ccggatgcgg tgtaaacacc ttatccggca tacagaacaa tactacaccg taattaattt
    28381 tacccgtgtc ttctgcaatg ccaatttatc cacctctgac acagcatcat cggtaacaat
    28441 aatgtcaatt ttttcgattg gcagtacctg attaaaaccg cgacgattaa atttcgatga
    28501 atcgagaacg gcaatcactt tatttgcggc ggtgaccatc gccccgctaa tcgaataacc
    28561 ttcattaaag gtcgtaatac cattaaccgc atcaatacca tcagcaccga caaacattaa
    28621 atcggcatta atatcctgca atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt
    28681 atggcggacc gtgccaccac agacgaccag agtaatgtct ttattttcgg aaagggcaaa
    28741 cgctgccggg agactgttgg taatcaccgt gatattttta gcggtcatta atccttcggc
    28801 gataagcatt gtggtacttc cgctgtcgag aataacggtc atcccttcat ggatcatttt
    28861 gactgccgcc tgcgctattc tgttttttgg cgcgctggcg agctgaaatc gctctttaaa
    28921 cccgacttcc tgggtctcgg tttcactatt accggacatt attttcgccg caccgccatg
    28981 aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc
    29041 cgaggcagca aatactcccg ccagatcctg aacaagcacg gttccctgtt cattcactaa
    29101 ttggatgatc ttattccttc gctcgaatga attcatcgtc atttacccat attcaattgt
    29161 ggctagtgta aacgaagggg gaccattggt gaatgatctg ttttgcacaa taccgggcca
    29221 gcatcacgct ggcccgcggt ttcatggaat gagcaacact ttgccctgca tggcattacg
    29281 agcgatgtca cgcaccgcct gggtgaagct ttcaaagctt ccacggtgag cgattaatgg
    29341 ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg
    29401 ccaagggctg gaatagttca tccaactgcc gataaccgtc agctctttac gcaatatttt
    29461 gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac gtgcccacca gcgccagttg
    29521 ggcatgagga ccggcaatct ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc
    29581 gaggataagc tgattaaagc gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat
    29641 ttcgctactg ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca gtttttctga
    29701 gctaatgtcg atcgccgtca cactctttgc tcccagcgcg acagcgcact gaatagccag
    29761 caggccaatg gttccggcac caataataat aacgttttta ttctcacaac cttgcgctaa
    29821 atgaaaagca tgcaggccaa cggtaatcgg ctcaataaaa gccccatcct caataggcat
    29881 atccgtgggt agagcaaaga cattttttcg cttaacgaca atatattcag caaatccacc
    29941 atcacgccgt gagccaataa aatcatattt tgcgcactgg gaataaaacc ctttcaaaca
    30001 ctctggacaa gtaaaacagg gtaataacgg cacacaggca accgcatcgc cagggtgtaa
    30061 atcatcaaca ccggatccca ccgcatcaat atagccgcta aattcatggc ctaacgttat
    30121 tggataataa tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac ataagcccga
    30181 gctggcaatt tttacccgca cctcatcctg atgtttaatt tcaggaatga cgctttttgc
    30241 aacgcgcacg ataccatcag tatcattcac tactgatttc ataaaaactc ctgatt
//
LOCUS       DAZSNO010000035        29469 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZSNO010000035 DAZSNO010000000
VERSION     DAZSNO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29469
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.035"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            71..352
                     /locus_tag="ACHWN2_001517"
     CDS             71..352
                     /locus_tag="ACHWN2_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR8931105.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            318..1395
                     /locus_tag="ACHWN2_001518"
                     /pseudo
     CDS             318..1395
                     /locus_tag="ACHWN2_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1788..2129)
                     /locus_tag="ACHWN2_001519"
     CDS             complement(1788..2129)
                     /locus_tag="ACHWN2_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR8931106.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2142..3014)
                     /gene="copD"
                     /locus_tag="ACHWN2_001520"
                     /pseudo
     CDS             complement(2142..3014)
                     /gene="copD"
                     /locus_tag="ACHWN2_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3018..3392)
                     /gene="yobA"
                     /locus_tag="ACHWN2_001521"
     CDS             complement(3018..3392)
                     /gene="yobA"
                     /locus_tag="ACHWN2_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR8931107.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3531..3761
                     /gene="holE"
                     /locus_tag="ACHWN2_001522"
     CDS             3531..3761
                     /gene="holE"
                     /locus_tag="ACHWN2_001522"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR8931108.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3863..4519
                     /gene="yobB"
                     /locus_tag="ACHWN2_001523"
                     /pseudo
     CDS             3863..4519
                     /gene="yobB"
                     /locus_tag="ACHWN2_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            4543..5205
                     /gene="exoX"
                     /locus_tag="ACHWN2_001524"
     CDS             4543..5205
                     /gene="exoX"
                     /locus_tag="ACHWN2_001524"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR8931109.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5202..7262)
                     /gene="ptrB"
                     /locus_tag="ACHWN2_001525"
     CDS             complement(5202..7262)
                     /gene="ptrB"
                     /locus_tag="ACHWN2_001525"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR8931110.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7472..8131)
                     /locus_tag="ACHWN2_001526"
     CDS             complement(7472..8131)
                     /locus_tag="ACHWN2_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR8931111.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8458..8814)
                     /gene="yebF"
                     /locus_tag="ACHWN2_001527"
     CDS             complement(8458..8814)
                     /gene="yebF"
                     /locus_tag="ACHWN2_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR8931112.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="ACHWN2_001528"
                     /pseudo
     CDS             complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="ACHWN2_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9305..10483
                     /gene="purT"
                     /locus_tag="ACHWN2_001529"
     CDS             9305..10483
                     /gene="purT"
                     /locus_tag="ACHWN2_001529"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR8931113.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKSDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10539..11180)
                     /gene="kdgA"
                     /locus_tag="ACHWN2_001530"
                     /gene_synonym="eda"
     CDS             complement(10539..11180)
                     /gene="kdgA"
                     /locus_tag="ACHWN2_001530"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR8931114.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11217..13028)
                     /gene="edd"
                     /locus_tag="ACHWN2_001531"
     CDS             complement(11217..13028)
                     /gene="edd"
                     /locus_tag="ACHWN2_001531"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR8931115.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13263..14738)
                     /gene="zwf"
                     /locus_tag="ACHWN2_001532"
     CDS             complement(13263..14738)
                     /gene="zwf"
                     /locus_tag="ACHWN2_001532"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR8931116.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15076..15945
                     /gene="hexR"
                     /locus_tag="ACHWN2_001533"
                     /gene_synonym="yebK"
     CDS             15076..15945
                     /gene="hexR"
                     /locus_tag="ACHWN2_001533"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR8931117.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16073..17515
                     /gene="pyk"
                     /locus_tag="ACHWN2_001534"
     CDS             16073..17515
                     /gene="pyk"
                     /locus_tag="ACHWN2_001534"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR8931118.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17646..18617)
                     /gene="lpxM"
                     /locus_tag="ACHWN2_001535"
                     /gene_synonym="msbB"
     CDS             complement(17646..18617)
                     /gene="lpxM"
                     /locus_tag="ACHWN2_001535"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR8931119.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18737..20059)
                     /gene="mepM"
                     /locus_tag="ACHWN2_001536"
     CDS             complement(18737..20059)
                     /gene="mepM"
                     /locus_tag="ACHWN2_001536"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR8931120.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20075..21007)
                     /gene="znuA"
                     /locus_tag="ACHWN2_001537"
     CDS             complement(20075..21007)
                     /gene="znuA"
                     /locus_tag="ACHWN2_001537"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR8931121.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21086..21841
                     /gene="znuC"
                     /locus_tag="ACHWN2_001538"
     CDS             21086..21841
                     /gene="znuC"
                     /locus_tag="ACHWN2_001538"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR8931122.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21838..22623
                     /gene="znuB"
                     /locus_tag="ACHWN2_001539"
     CDS             21838..22623
                     /gene="znuB"
                     /locus_tag="ACHWN2_001539"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR8931123.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22770..23780)
                     /gene="ruvB"
                     /locus_tag="ACHWN2_001540"
     CDS             complement(22770..23780)
                     /gene="ruvB"
                     /locus_tag="ACHWN2_001540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR8931124.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23789..24400)
                     /gene="ruvA"
                     /locus_tag="ACHWN2_001541"
     CDS             complement(23789..24400)
                     /gene="ruvA"
                     /locus_tag="ACHWN2_001541"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR8931125.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24539..24604)
                     /gene="yobI"
                     /locus_tag="ACHWN2_001542"
     CDS             complement(24539..24604)
                     /gene="yobI"
                     /locus_tag="ACHWN2_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFR8931126.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24674..25276
                     /locus_tag="ACHWN2_001543"
     CDS             24674..25276
                     /locus_tag="ACHWN2_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR8931127.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(25278..25799)
                     /gene="ruvC"
                     /locus_tag="ACHWN2_001544"
     CDS             complement(25278..25799)
                     /gene="ruvC"
                     /locus_tag="ACHWN2_001544"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR8931128.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25834..26574)
                     /locus_tag="ACHWN2_001545"
     CDS             complement(25834..26574)
                     /locus_tag="ACHWN2_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8931129.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26603..27055)
                     /gene="nudB"
                     /locus_tag="ACHWN2_001546"
     CDS             complement(26603..27055)
                     /gene="nudB"
                     /locus_tag="ACHWN2_001546"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR8931130.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(27173..28945)
                     /gene="aspS"
                     /locus_tag="ACHWN2_001547"
     CDS             complement(27173..28945)
                     /gene="aspS"
                     /locus_tag="ACHWN2_001547"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR8931131.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            29255..>29469
                     /locus_tag="ACHWN2_001548"
     CDS             29255..>29469
                     /locus_tag="ACHWN2_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFR8931132.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDA"
BASE COUNT         7227 a         7571 c         7235 g         7436 t
ORIGIN      
        1 tattaaaaat caaccacacc cgccagtatt ctgtatattt actggcggtc atatcgtgag
       61 aggtatggca atgaatcttg tgacactcaa aacgtgggga aaactcagat atccggataa
      121 cccaccatca atatcaacgc tgagacgatg ggcaaggaat ggaaacattt atcctgcacc
      181 tgaactacac gggaggagtt acagggtggt tccggaggct ttctatatca acccaaataa
      241 ggttgatacc gatataacac accatcagcc taatgggcga caagggagag acagtccgtt
      301 aatggagaag ttaaaacatg cagcggaaaa aatacgatcc caatttgcct agaaacttaa
      361 catatcgaag gagagacaaa gcatattact ggcgcaaccc tctgacgaaa gaagaattta
      421 cactaggtaa aatttcaaga agagatgcag tagcgcaggc aattgaagca aatcattata
      481 tatacaaaaa ctactctcct gctgccctaa ttgaaaagct taaaggggtc gactcattta
      541 ctatggcagg ctggattgaa cgttacaaaa cgattcttat aaggagaaaa gcgtccagaa
      601 atacttataa aactcgggta aatcaactgg agacaataaa agaaaaatta ggaggggttt
      661 tactgacaga aataaccact cgccatatcg ccgagtttct tgatttgtag attgaaggag
      721 ggaaaaacac gatggcagga tcaatgcgtt ctgtgttatc tgatatgttc cgcgaagcca
      781 ttgttgaagg acgtatatct caaaatccag taacgccaac aagagcaccg aaaatagtag
      841 ttacaagaga acggctgaaa ctaaagacat acaactgcat cagggaggca gcagatcaac
      901 ttccggcatg gttcccatta gctatggact tagcccttgt aacaggacaa cgtcgcgaag
      961 acataacgaa tatgcggttc agtgatattt atgatgatcg tctccacatc aggcaaatta
     1021 agacaggaat gatgattgct atccccctgt cactcagcct tcctgtcgct ggtttacgac
     1081 ttggtgcagt agttgaacag tgccgcatgg taagtagggg ggattatcta atcagtgccg
     1141 ggattagaaa aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg
     1201 tcgcagccag aaaattaaca ggtatcactg ttcagtgaaa acccaccaac ttttcacgaa
     1261 atcagaagcc tggctggacg attgtacaaa gaaatatgtg gagaagaatt tgctcagcgt
     1321 ctacttggcc acacatcgga gaagacaaca aaaatgtatc ttgatgagag agaaaaaacg
     1381 tacttactgc tctgatttta acgtaaatgg attgttaaat gtattttggt tgtgatataa
     1441 ccaaaaaaga ccggaataca gaaattcgag taaatttcgg ggaatttcgg ggagacgttt
     1501 gcaactaatt gattttaaat acaattaaaa aaagaccgaa tacgattcct gtattcggtc
     1561 cagggaaatg gctcttggga gagagccgtg cgctaaaagt tggcattaat gcaggcttag
     1621 ttgccttgcc ctttaagaat agatgacgac gccaggtttt ccagtttgcg tgcaaaatgg
     1681 tcaataaaaa gcgcggtgct catcagctta aatgttaaaa accgcccgtt ctggtgaaag
     1741 aactgaggcg gtttttttat tggaaatcaa aaggctattt taggtaatta acagagtttt
     1801 tcagctcgtt ctataaacgg tgccagactc attttttcgc cgggattgtt aggatcatca
     1861 atctgaatca ccgaaatggg ttgggcattg gtcttcccac tggcaacttc cttttgtgcg
     1921 atatcgttta aaggatactg cacgagggta cttgggttga tgacatacaa agcattaccc
     1981 ggtcggcaag tcagcattac ctcttcgcga ttaaacgccc atttgtcttt acccacttca
     2041 aaacgactca cggttatcac ctgtggagcc gccagcgccg ctgcagaact ggtgagtaac
     2101 agaaacgcca gaatactttt tttcatcatt ttcgtaatcc atcaaaaagg ttcgagagtc
     2161 gcaagcaggc tgatgatcac cagcactacc gcacctatcg cccattcgag tttagtcatc
     2221 cacacaaacc agggtgccgc acgatcttca tcctgcctca tgcgcggtac aagaacataa
     2281 cgattagcca gcgcaatgac caccataatc atcaccagga tggctttgag caataagagt
     2341 tggccccagt aagtcgtaag cgtgggggga aatccggtaa tcaacaatgc attaagcacg
     2401 ccgcttgcca gtacgccgat caccgcaaaa tgcccgcacc aggaaaaacg catcagcgcc
     2461 tgaatagcct agtgtcgcca acggcctttg atgagttgca tacaccagag caccggcagt
     2521 aatcccccaa accacgccgc cgcacaaatc aggtgaatcg cgtgattagt ctgatggatt
     2581 ttcgctgtta ccccttcatt cagcgtcgca taccccactc ccgccagcag aataaattgc
     2641 gcggtagtga gcataaacag caaacgtggc atattccgcg gttgcataag ggcgacgatc
     2701 aacgttacga gggcgagaac aatttgccat aaccagacgc caccaaactg cgtttgcaaa
     2761 acagcctgcc aaatgtttgg cgaaaataca tctgtccatc ccgttcccat caacccgccc
     2821 tgaacggcga gcattgcagt ggcactgatc aaactccaga cggcagcgtg ctgttgtaaa
     2881 cgcagagaac gcttcgtcag caagcgacga atcattaagg gggccagcca ggcaccatac
     2941 attgcaaagc caaagaccag catcaacgag gtgaaatgga taaatcgcag cgcgatccag
     3001 gtaaacgcca gcatgattta tttcacgcta aaggtgtagt gtcctttcgt tttatgcccg
     3061 tccaccgaaa caacatgcca gtcaacggtg taggttccgg gtttcagcga atcggccagt
     3121 ggaacaatca gttgcttttg gtcctgctca ttccgcttcg ctggtaatgt tttaatattt
     3181 tcgttttttg gccccgtgat ttttgcacca ctgaatcctg tttcaacgcc ttccgagaag
     3241 tttaaagtga ttgcctgcgg ggcagctgtc acttgcgcgt ttgccgcagg atactgatgc
     3301 gttaaatgcg catgtgccca gacggaaggt gtcaccaacg aagtggtcag gattgccagc
     3361 gcgtagcgaa gggagcgtgc agttgaagcc atattatcta ttcctttttg taataacttt
     3421 tttacagagc ataaccttgt ctaatgtctg agtcgaggat catcaattcc ggcttgccat
     3481 ccaggctcac tcttagtaac ttttgcccgc gaatgatgag gagattaaga atgctgaaga
     3541 atctggctaa actggatcaa acagaaatgg ataaagtgaa tgtcgatttg gcggcggccg
     3601 gggtggcttt taaagaacgc tacaatatgc cggtgatcgc tgaagcggtt gaacgtgaac
     3661 agccagaaca tttgcgcagc tggtttcgcg agcggcttat tgcccaccgt ttggcttcgg
     3721 tcaatctgtc acgtttacct tacgagccca aacttaaata aaacttatac agagttacac
     3781 tttcttacat aacgcctgct aaattatgag tgttttctaa accgccccca taatttgcag
     3841 tcattttgaa aaggaagtca ttatgtcgtt ctggaaagtt gcagcagcgc aatatgagcc
     3901 tcgtaaaacc tcactcaccg aacaggtagc tcatcatctg gagtttgtga gggccgccgc
     3961 caggcaacaa tgtcagctgt tagtttttcc atcgctttct ttactggggt gtgattattc
     4021 gcgacgtgcc cttcctgccc cacccgatct ttcactgtta gacccgcttt gttatgccgc
     4081 aacgacctag cgaatgacca tcattgccgg ccttcccgtt gaatataacg atcgctttat
     4141 tcgtggaatt gcggtgttcg caccgtggcg aaaaacgcct gggaactacc atcagagtca
     4201 tggtgcatgt ctgggccgtc gttccagaac catcacggtg gtcgatgaac aaccgcaagg
     4261 tatggatatg gacccgacct gttcactgtt tacgacaggg caatgtctgg gagaacccga
     4321 cctgctggcg tcggcccgtc gcttacaatt tttttcacat cagtactcga ttgccgtgct
     4381 gatggcaaat gcccgtggta acagtgcact atgggatgaa tatggtcgtc tcatcgttcg
     4441 cgccgatcgt ggttcattat tgttagtcgg tcagcgttca tcacagggtt ggcaaggcga
     4501 tatcattcca ttacgctagg ctttttcggc ctggatcatg ccatgttgcg cattatcgat
     4561 acagaaacct gcggtttgca gggagggatc gttgagattg cctctgttga tgtcattgac
     4621 ggaaaaatcg tcaaccccat gagcctcctg gtgcgccccg atcgtcctat tagtccacaa
     4681 gcgatggcga ttcatcgcat caccgaagcc atggtcgccg ataaaccgtg gattgaagat
     4741 gtgatcccac actattacgg tagtgaatgg tatgtcgcgc acaacgccag ctttgaccgc
     4801 cgcgtactgc ctgagatgcc cggtgagtgg atttgcacta tgaaactggc ccgtcgtttg
     4861 tggccagggc tcaagtacag caatatggcg ttatataaaa cacgcaagct caatgtacag
     4921 acaccgctgg gcctgcatca tcaccgcgcg ttgtatgact gttatatcac cgccgcgttg
     4981 cttatcgata ttatgaacac ctccggctgg acggcagaac agatggccga tatcaccgga
     5041 cgtccgtcgt tgatgacaac cttcaccttt ggcaaatacc gtggcaaagc ggtttccgac
     5101 gttgccgaac gctatccggg ctatctgcgc tggttattta ataacctgga cagcatgagc
     5161 ccggagctgc gtttaacact gaaacattat ctggaaaata cttaatccgc aggcgtagcg
     5221 ggtaatgttc cctgcgccag cgcgaccaga aaagcatatt ccatcgctac gccttcgtac
     5281 gatttaaagc ggccagattt accgccatgg cctgagtcca tgtcggtaca gagcaataaa
     5341 agatggtcat cggttttcag ctcgcgcaat ttagcgaccc attttgccgg ttcccaatat
     5401 tgcacctgag aatcgtgtaa accggtcgtt accagtaaat gcggataagc ctgtgcggtg
     5461 acgttgtcat acgggctgta gcttttcatg tactcgtaat attgcggatc ctgcggattt
     5521 ccccactctt caaactcacc agtggtaaga ggaattgatt catcaagcat cgttgttaca
     5581 acatcaacaa acggtacctg ggcgataacg ccgtggaata attccgggcg ttgattaatt
     5641 gcaacgccca tcaacatgcc ccccgcactt ccgcccatcg cataacagag cgaaggagag
     5701 ccatagccca gttttaacaa tgcatcgcag gcatcaagat aatcattaaa cgtatttttc
     5761 ttcttcagaa atttaccgtc ttcgtaccat tgttgcccca gctcaccacc gccgcgaaca
     5821 tggacaatgg cgtagacaaa gccacgatct aacaaactca agcggctaaa actgaaatcg
     5881 gcatcaatac ttgcgccgta agaaccatag ccatacacca gcaacgggtt gtgtcctttg
     5941 cgaaaatgtt tgcgatgata gaccagcgaa accggaactt cgacgccatc acgggcgact
     6001 atccacaggt gttcactgcg gtaattcgcc gcatcaaaac caggcacttc tgtttgtttt
     6061 aatacacgac gctcaccggt atccatatcc agttcaaaca aagtgtctgg tgtggtcatg
     6121 gaagaataac cataacgcaa tcgcgcggtt tcaggttctg gattgtaggc aatccaggtc
     6181 acataggccg gatcatcaaa ggcaatgcca atgacttccc gggtcttgcg gttaatttgg
     6241 cgcaaactgg ttaacccgcg ctgacgctct tcaaccacca gccagtcggt aaacagcgta
     6301 aacccttcca gcatgatgtt ttcgcgtggc ggaattaact cttcccactg ttgctcatca
     6361 cgcatacggg tacggtataa gccaaagttt ttgccgtggc gattggaacg cagataaaaa
     6421 cgatgctggt agtgatcaag gctgtattcg tgatctttgc ggcgcggcag aaaaacaaac
     6481 ggctcggcat cggccatttc cgcgtccagc aggcgaactt cactggtggt ggcgctggcc
     6541 agatgaatga cgacatagtg cttcgacgtc gttttatgca ggctgacata ataggtatcg
     6601 tctttttctt cgtagatcag tgtatcttgc gatgctggcg taccgatggc gtgacgccag
     6661 acctgataag gcagcagcgt caccggatgc ttgcgaacat agtagaaagt ccaggagtca
     6721 tttgcccaga caaagctggg ttcaacgtta tccagcagtt ccgggtacca gttacctgtt
     6781 tccagattac gaaaacgaat gccgtactgg cgtcgggaaa gaaaatcttc tgccagcgcc
     6841 ataatggtgt tatcgggcgt aatcgccatt ccgcccatcg aataaaactc actatgagct
     6901 gcgcgcttgt tggcatcgag caatgtttcc cactcatccc actcttcgct gaatgccgat
     6961 tgacgctggt agatagcata ttcacagcct ggttcataaa tatgccgata gcggtagcca
     7021 ttttttatgt agggcgcaga aacttctctt tgcggaatgc ggtcgatgat ttcctttaag
     7081 atgcgatcct gcaaggcttg ttgtgaggcc atcacccgat gaccgtaact attttcttgt
     7141 tgcaggtagt ccaggacttt tggctgagaa cgcgtatcgt cccgcagcca gtagtaatta
     7201 tcgatgcgcg tatcgccatg aagcgtcatg gcgtggggaa tgcgggcggc ttttggtagc
     7261 atgttattgt tctttctggt tggaaacatc ttataagggt ggcaaaactc accggggatg
     7321 caagcgaaac agggtcgtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg
     7381 ttatgcagac agagaaaatc agcctgttca aatggctgtg cggttctgga tagcccgaaa
     7441 tagtcaactt caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt
     7501 cgagatcgcg ttcaatgcct tcacgaacat cctgggggat tttcagcgcg tcacccagtg
     7561 cattcaggta actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat
     7621 agatttccag cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg
     7681 gttgttcgat tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca
     7741 attgctggtc gattgccgca cgttcattgg catcaatatg accatcactt ttagcggcaa
     7801 agaccagcgc aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa
     7861 actgcggttc gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg
     7921 tacccgctac cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt
     7981 tacgtgctga tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctgacacta
     8041 acagtttgcc caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca
     8101 gggattgcag ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc
     8161 ctgtagcata gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata
     8221 agcggggcgg taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat
     8281 caggtgataa gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg
     8341 ctgattgccc gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg
     8401 gacggacaag gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta
     8461 acgccgctga tattccgcca ttcccgcttt gcagtccacg cagacctggt aatgaatatc
     8521 ggcactttta ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc
     8581 ctgcaaactg acccaggcca cgggatcggc ctgaccgaca attttttgat catctgccca
     8641 gcgcgccacg cgattttgtt gataatcacg ttttacgctc gcggcaattc cggcagcatc
     8701 cagaccttca cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac
     8761 tgatgcgcag gcagaaacca acaaccgccc taaaaacgcc cctctttttt tcatgttttt
     8821 ctccatagca caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct
     8881 tcaggccgcg tgtgaggcat cttcctcttc ggattcagca ccgacgacct gtggggacgg
     8941 taatttgccg gttttcagaa tggtgctcag cacctctttt tgttctgcca gccatagcga
     9001 aagggcttca cgttgctcgt cttccatttg cactggagaa tttgtcagct aggtgtcaag
     9061 cagatccgcc gtatcaagca ttttgtcata agcatcggct tcctttttgc tggtaaacga
     9121 cattttctct tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct
     9181 ctcataataa ctgaaatttt atacagtata tttcttttcg gttgagaaat caacatcagc
     9241 aataaagaca cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt
     9301 cgttatgacg ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg
     9361 ctccggtgaa ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat
     9421 tgccgtcgat cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat
     9481 taatatgctt gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat
     9541 cgtgccggag atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact
     9601 gaatgttgtc ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg
     9661 cctggcggca gaagagctgc agctgcccac ttccacttat cgttttgccg atagcaaaag
     9721 ccttttccgc gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag
     9781 ctcttccggc aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga
     9841 atacgctcag caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa
     9901 gtctgacttc gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc
     9961 accagtaggt catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat
    10021 gagcccgctt gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg
    10081 cggttatggt ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga
    10141 ggtctcccct cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga
    10201 gtttgccctg catgtacgtg ccttcctcgg acttcctgtt ggcgggatcc gtcagtatgg
    10261 tcctgcagct tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa
    10321 tgtgcagaat gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga
    10381 tggcagccgt catctggggg tggtactggc tacttcagag agtgttgttg acgccattga
    10441 acgcgcgaag cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaaacgc
    10501 tacaaaaatg cccgatccta gatcgggcat tttgactttt acagcttagc gccttctaca
    10561 gcttcacgcg ccagcttagt aatgcggtcg taatcgcccg cttccagcgc atctgccgga
    10621 accagccagg aaccaccgat gcacagcacg cttttcagtg ccaggtagtc acggtagtta
    10681 gccggagaaa taccacccgt cgggcagaaa cggacctggg agaacggacc cgcgatcgcc
    10741 tgcagggctt tcacgccgcc gttagcttca gccgggaaga atttgaactc tttcaaaccg
    10801 tagtccatac ccagcatcag ttcggaaaca gtgctgatcc ccggaatcag aggaatagtc
    10861 ccttcggtag cagctttcag cagcggctcg gtcagacccg ggctaattgc gaactgtgca
    10921 cccgcttcag tgacttctgc cagctgctgt ggattcagca ccgtaccggc acccacaatc
    10981 gcttcaggca cttctttggc gatagcacgg atagcgtcaa ctgcacactc ggtacgcaga
    11041 gtcacttcca gaacgcgcac cccaccagca accaacgctt ttgccatcgg caccgcgtgt
    11101 tccagttttt ttaccacgat aaccggtaca accgggccgg tggtcaggat tgattctgca
    11161 cttgttttcc agtttttcat cagagttttc tctcgcctga ttacaaattt gtcgtcttaa
    11221 aaagtgatac aggttgcgcc ctgttcggca ccggacagtt tttcacgcaa ggcgctgaat
    11281 aattcacgtc ctgttcccac gcgtgacgcg ctcaggtcag gaatgtgcgg ttcgcgagca
    11341 gccagttccg cttcgtctac cagcagtgtc agttcgcctg tctgtccatt cacacgaatg
    11401 atgtccccgt cgcgcacttt tgccagcagc ccgccatcgt aggcttctgg tgttacgtgg
    11461 atagctgacg gcactttacc tgaagcgccg gagagtcgtc catcggtaac taacgcaatt
    11521 ttgaaacacc ggtccaataa tacaccaagt ggcggcatga gtttatgtaa ttctggcatt
    11581 ccgttcgctt ttggcccctg atgacggaca acaacgacac aatcgcggtc cagcaaaccc
    11641 gcttcaaagg ccggcataac gtcatgctgg ctttcaaaaa caaccgctgg cgcttcaatc
    11701 acctggttct caaccggcac ggcagaggtt ttcataaccg cacggcccag gttaccgctt
    11761 aacacttttg tcccaccatg atgagagaaa ggttgttcga aggaagcgat cacattgctg
    11821 tcgagtgatt tttccgcccc ttcccgccag tccagttcac cattattcag ccatggttca
    11881 agggtataac gagacagacc aaagcctgcc accgtattga catcttcatg cagcaggcct
    11941 gctttgagca gttcacgcac cagaaccggt acgccacctg ccgcctggaa gtggttaata
    12001 tcggccggac cgttcgggta gagacgtgcc atcagcggta caacatcaga aaggtcagag
    12061 aagtcatccc agttaatctg aataccggcc gcgcgcgcca tcgccaccag gtgcatggtg
    12121 tggttagtgg aaccaccggt cgccagcagt gcaacgatac cgttcaccac cactttctca
    12181 tcgatcatct taccgatcgg catccattca ttaccattac cggtcatgcg tgtaacctga
    12241 cgcgcagcgg cagcggtcaa agcatcgcgc agcggagaat ccggatgaac aaaagaagag
    12301 cctggcaact gcatccccat aaattccacc accatctggt tggtgttggc agtaccgtag
    12361 aaagtacatg ttcccggcgc atggtaagac gcggcttctg actccagtaa ggccatgcgg
    12421 tccactttac cttcggcata aagctggcga atacgcactt tttctttatt tggcaaaccg
    12481 cttgccatcg gtccagacgg cacaaacacc gcaggcaaat gaccaaacga cagggctgcc
    12541 atcgtcagac ccgggacaat cttgtcgcac acaccgagga acagagcacc atcaaacatg
    12601 ttatgggaca gccccaccgc cgcagacatc gctatcactt cgcggcttag cagcgacaat
    12661 tccattccat cctgcccctg ggtgacacca tcacacatcg ccggaacacc gcccgcaacc
    12721 tgaccaaccg cattcgcttc atgcagggct ttacgaatga tttctggata gtgttcataa
    12781 ggctggtgcg cggagagcat gtcgttatag gaggtgatga tggcgatatt gttacgcaac
    12841 atgcttttca aagaggcttt gttttctggc tggcaggcag cgaaaccgtg tgccaggtta
    12901 ccgcatgcca actgcgaacg atgaacggtc gacgttttcg cttgttctat ccgggcgaga
    12961 taagcagagc gagtctcgcg cgaacgttca atgattcgat ttgttacgcg taacaattgt
    13021 ggattcataa aggctcctga aattgagttg tcagagcagg atgattcaca acgcgtttca
    13081 ttcagaggat ttatgagtga aacgcctgta accggagctc atagggcaaa aacgtttcag
    13141 tcagtgtaat aaaaaaagcc tcgtgggtga atccgcacga ggcctgaaag tgtaaaaatt
    13201 gttctacaat ctgtgcaaga tcatgttacc ggtaaaataa ccataaagga taagcgctga
    13261 tattactcaa actcattcca ggaacgacca tcacgggtaa tcatcgccac cgaggcaacg
    13321 ggtccccagg ttccggcctg atacggtttc ggcgcatcat tgtccatcgc ccacgcctca
    13381 gtaatggagt ctacccattt ccaggcttct tccacttcgt cgcgacgtac aaacagtgcc
    13441 tgaataccac gcatggtttc cagcagcaaa cgttcatagg catccgccag atgcgtctga
    13501 ttaaaggttt ctgaatagct cagatccagc ttggtgattt gcaggttatg tttgtggtca
    13561 aggccaggaa ctttattcag tacctggata tccacgcctt catcaggttg cagacggata
    13621 gtcagtttat tctgcggcag atcctgccac gactctttaa acagattcag ttcaggtgtt
    13681 ttgaaataga ccacgacttc agaacatttg gtcggcagac gtttaccagt acgcaggtag
    13741 aatggcacac cggcccagcg ccagttatca atgtcgacgc ggatcgccac gaaggtttct
    13801 gtattgctgc ttttgttcgc gccctcttct tccagatatc ccggcacttt tttgccctgg
    13861 gcgaagcccg cagtatattg cccgcgtacg gttttttcgc gtacgttgga gcggtcgatg
    13921 cggcgcaggg acttcagtac tttcactttt tcatcgcgga tgctgtctgc gctcaggtca
    13981 gatggcggag acatcgcaat catgcagaga atttgtagca ggtggttctg aatcatgtca
    14041 cgcatctgac cggctttatc aaaatagccc cagcgccctt cgatccccac ttcttctgcc
    14101 acggtaatct caacatgatc aatggtgcga ttgtcccagt tattcacaaa cagggagtta
    14161 gcaaaacgca gcgccaacag gttcagcacc gtttctttac caagatagtg gtcgatacgg
    14221 taaacctggc actcctcgaa gtattcgcca acctgatcat tgatttcctg cgaggtcgcc
    14281 agcgacgtcc ccagcggttt ctccatgact acgcgtgccg gtttagcatt cagttttgcc
    14341 tcgccaagcc ctttgcaaat tgcgccaaaa gtgctgggcg gcatggcaaa gtagttaata
    14401 gtgatacgat ttttttgatc cagcatcgcg ccgagacggt tgaatgcagc agtgtcattg
    14461 acatcgagat tacaaaaatc cagacgtgca ctcagggtgt cccataaacc ttcatcaatg
    14521 gtttctttca tgaaagtttc gagcgcctcg cggacaactt tggtatatgc cgctttatcc
    14581 cagtcagcac gccctacgcc gataatccgg gtgtccgggt tgagctgacc gactttttcc
    14641 agttgataca gggaaggcag caatttacga cgcgcaaggt cgcctttcgc gccgaaaatg
    14701 accaggtcac aggcctgggc tgtttgcgtt accgccatgt cattctcctt aagttaacta
    14761 acccggtact taagccaggg tatacttgta attttcttac ggtgcactgt actgctttta
    14821 cgagcttgcg aaaactgtaa acgcttatcc acccgtgcga ttacgggaaa agcgcgcaaa
    14881 gcgcggcaaa actgataaaa aaatcatcgt tttccattcg gtaaaaatct gacactgatc
    14941 atgttatgaa aaaaaataac aactttttta tctgcttttg tcattaacac ggcacacagg
    15001 cgtaatatct gacaaaactg catttcgatt tctttcagtg cggaaatcgt cattacccgt
    15061 gagtctcttt acatcatgaa tatgctggaa aaaatccagt ctcagctgga acatttgagc
    15121 aaatcagagc gcaaagttgc cgaggtcatt ctggcttcgc ccgataacgc gatccattcg
    15181 agtattgctg ctctggcgct ggaagccaat gttagcgaac cgacggtgaa tcgtttctgt
    15241 cgcagcatgg acacgcgcgg ttttcctgat tttaaacttc atctggcaca aagtctggcg
    15301 aatggcactc cctatgttaa tcgcaacgtc aatgaagatg acagcgttga atcatacacg
    15361 gggaaaatat ttgagtccgc aatggcaacg ctcgatcatg tccgtcattc actggataaa
    15421 tctgccatca accgcgccgt cgacttgctc actcaggcaa aaaaaatcgc ctttttcgga
    15481 ttaggctctt ctgccgccgt tgcccacgat gcgatgaata agttctttcg ttttaatgtt
    15541 ccggtggtgt actccgatga tatcgtgctg caacgcatga gttgtatgaa ttgtagcgac
    15601 ggagacgtgg tggtgctgat ttctcacaca ggaagaacaa aaaatctggt cgagctggcg
    15661 cagctggcac gcgaaaacga cgccatggtg attgccctca cctctgcggg taccccgctc
    15721 gcccgggaag caacgctggc aattaccctc gacgtaccgg aagatactga catttatatg
    15781 cccatggttt ctcgacttgc acagctgacc gtgatagatg tgctggcgac aggatttact
    15841 ttgcgacgcg gtgcaaaatt cagagataac ttgaagcggg tcaaggaagc gctgaaggaa
    15901 tcgcgttttg ataagcagtt acttaattta agtgacgatc gctaaaagcg actgtcactg
    15961 tcctaatctt atacgacatc cgaatgagat taatttatcg ccatcgcggc gttatttcat
    16021 tcggatttca tgttcaagca acacctggtt gtttcagtca acggagtatt acatgtccaa
    16081 aaggcttcgc agaacaaaaa tcgttaccac gttaggccca gcaacagatc gcgataataa
    16141 tcttgaaaaa gttatcgcgg cgggtgccaa cgttgtacgt atgaactttt ctcacggctc
    16201 gcctgaagat cacaaaatgc gcgcggataa agttcgtgag attgccgcaa aactggggcg
    16261 tcatgtggct attctgggtg acctccaggg gcccaaaatc cgtgtatcca cctttaaaga
    16321 aggcaaagtt ttcctcaata ttggggataa attcctgctc gacgccaacc tgggtaaagg
    16381 tgaaggcgac aaagaaaaag tcggtatcga ctacaaaggc ctgcctactg acgtcgtgcc
    16441 tggtgacatc ctgctgctgg acgatggtcg cgtccagtta aaagtactgg aagttcaggg
    16501 catgaaagtg ttcaccgaag tcaccgtcgg tggtcccctc tccaacaata aaggtatcaa
    16561 caaacttggc ggcggtttgt cggctgaagc gctgaccgaa aaagacaaag cagacattaa
    16621 gactgcggcg ttgattggcg tagattacct ggctgtctcc ttcccacgct gtggcgaaga
    16681 tctgaactat gcccgtcgcc tggcacgcga tgcaggatgt gatgcgaaaa ttgttgccaa
    16741 ggttgaacgt gcggaagccg tttgcagcca ggatgcaatg gatgacatca tcctcgcctc
    16801 tgacgtggta atggttgcac gtggcgacct cggtgtggaa attggcgacc cggaactggt
    16861 cggcattcag aaagcgttga tccgtcgtgc gcgtcagcta aaccgagcgg taatcacggc
    16921 gacccagatg atggagtcaa tgattactaa cccgatgccg acgcgtgcag aagtcatgga
    16981 cgtagcaaac gccgttctgg atggtactga cgctgtgatg ctgtctgcag aaactgccgc
    17041 tgggcagtat ccgtcagaaa ctgttgcagc catggcgcgc gtttgcctgg gtgcggaaaa
    17101 aatcccgagc atcaacgttt ctaaacaccg tctggacgtt cagttcgaca atgtggaaga
    17161 agctattgcc atgtcggcaa tgtacgcagc taaccacctg aagggcgtta cggcgatcat
    17221 taccatgacc gaatcgggtc gtaccgcgct gatgacctcc cgtatcagct ctggtctacc
    17281 aattttcgcc atgtcgcgcc atgaacgtac gctgaacctg actgctctct atcgtggcgt
    17341 tacgccggtg ccctttgata gcgctaatga cggcgtagca gctgccagcg aagcggttaa
    17401 tctgctgcgc gataaaggtt acttgatgtc tggtgacctg gtgattgtca cccaaggcga
    17461 cgtgatgagt accgtgggtt ctactaatac cacgcgtatt ttaacggtag agtaagtacg
    17521 ttgccggatg cggcgaaaac gccacatccg gcctatagtt caatgatagt tcaacagatt
    17581 tcgaatattc tgaagcaaac ttgaacttat catcaggcga aggcctctcc tcgcgagagg
    17641 cttttttatt tgatgggata aagatctttg cgcttatacg gctggatttc gcccggtttg
    17701 cgagttttca gcaattttag tatccaggtg tattgttctg gtcgcggacc aacaaaaatc
    17761 tcgacttctt cattcatccg ccgctcaatc gtatgatcat ccgcctctaa cagatcatcc
    17821 atcggtgggc gcacctgaat cgtcagacga tgcgtcttgc catcataaat cggaaacagc
    17881 ggtacaacgc gcgcacggca tactttcatc aaacgaccaa tcgcgggcaa cgtcgcttta
    17941 taggtggcaa agaaatccac aaattcgctg tgctctgggc catgatcctg atcgggtaaa
    18001 taatatcccc agtacccctg acgtaccgac tggatgaatg gtttaatacc gtcatttctc
    18061 gcatgcagac gaccgccaaa gcgacgacgc accgtgttcc agacataatc aaaaaccggg
    18121 ttgccctgat tatggaacat cgctgccatt ttctgccctt gcgaggccat cagcatggca
    18181 ggaatatcga cggcccaacc gtgcggcacc agaaagataa ctttctcgtt attacgccgc
    18241 atctcttcga tgatctccag cccttgccag ccaacgcgcg gctgaatttt ctccggcccg
    18301 cgtattgcca actcagccat cattgccatc gcttgcggcg cggtggcaaa catctcatca
    18361 acaatcgctt cgcgttcagc ttcactacgt tctggaaagc agagcgacag attgattaac
    18421 gcacgacggc gtgagctttt tcccagtcgt ccggcaaaac gtcccagccg tgccagaatg
    18481 ggatcacgga actttggcgg cgttaaagcg atacccgcca tcgctgctac acccagccat
    18541 gctccccagt agcgcgggtg gcgaaaggat ttatcaaact caggaatgta ttcgctatta
    18601 ttttttttcg tttccatgct tttccagttt cggataaggc aaaaatcaat ctggtgatag
    18661 tgtagcggca caacttgccc cgcaccaaat aaaaaaagcc ggtactgact gcgtaccggc
    18721 tgcgaatgga tgttaattaa tcaaaccgta gctgcggcac aatctctttg gcctgcgcca
    18781 ggaattcgcg acgatcggag ccggtcagcc cttcggtacg cggcagtttt gccgtcagcg
    18841 ggtttacggc ctgctggttt atccatactt catagtgcag atgcggccca gttgaacgtc
    18901 cggtattacc ggaaagcgcg atacggtcgc cacgtttcac cttctgtccc ggtttcacca
    18961 gaatcttgcg caagtgcata taacgcgtgg tgtagctgcg accatgacga atagccacat
    19021 aataacctgc tgcgccactg cgtttggcaa ccaccacttc accgtcaccc actgaaagca
    19081 ctggcgtacc ttgtggcatg gcgaaatcaa cacctctgtg tggtgcaacg cgacctgtca
    19141 ccggattagt tcgacgcggg ttaaagttag acgagatacg gaactgtttc gccgtcggga
    19201 atcgcaagaa tcctttcgcc agaccagtac cgttacgatc gtagaatttg ccatcctcag
    19261 cgcggattgc gtaataatct ttaccttctg aacgcaaacg tacgcccagc agctggcttt
    19321 gctcacgttt accatcaagc atttctcgag acattaatac cgcaaattca tcgccttttt
    19381 tcagtttgcg gaaatccatt tgccactgca tggctttaat cactgcgctc acttcggcgc
    19441 tggttaaacc ggcgtttctg gcgctggcaa caaagctccc tccgacggta cctttcagca
    19501 gattgttaac ccactctcct tgctgcattt cgctggtcat tttaaaaccg ttagcggcag
    19561 tacggtcata ggttcgggtt tcacgacgag acacttccca ggtgaggcgc tgcagttcgc
    19621 cgtccgcggt taatgtccag gagagttgtt gaccgatttt caggttacgc aattctttgt
    19681 cggcagcagc aagttgggtg atatcaccca tatcaatacc atactgattg agaatgctgc
    19741 ttagcgtatc gccagtggaa acaacatatt catgcacgcc cgcttcaccg gcgattttgt
    19801 catccagttc gtcctgggga atggcttcat cttcttgtgc agcttgatca atcggctcac
    19861 tggcttcagg taagagcgaa cgaatttcgt tctgttccag ctcaatggtt ttgacaattg
    19921 gcgtggcatc acggtgataa acatagggcc gccagacagc gacggccaga gtaagaacgg
    19981 tgagcgaccc caacataacg cggtgtggtc gcggtaaatt attaaacgcc agggcgacag
    20041 agcgggctat ctgttgcacg taatcacttc ctcattaatc tcctttcagg cagctcgcat
    20101 actggttggc taattgactc aggaattctg aatagcttgt tttacccagt ttgatattcg
    20161 tccccagggg atccaacgtt cccatacgaa cggatgtccc tcgtgcgacg ctctcaacga
    20221 ccgctggcct gaactgtggc tcagcaaaaa cgcaggttgc tttttgctca accaactgtg
    20281 ttcttatttc atgtaaacgc tgcgcgccag gttgaatctc agggttaacg gtaaaatgac
    20341 caagcggtgt cagtccgaac tgtttttcga aatagccgta agcatcgtga aaaacgaaat
    20401 aacctttccc cttgagcggc gcgagctcgt taccaacctg cgtttcggtt gaggccaatt
    20461 gtgcctcaaa atccttcagg ttggcgtcaa gtttggctcg actttgcggc ataagttcca
    20521 ctaattttcc atggattgca accgctgtag cccgcgctat ctctggggaa agccaaagat
    20581 gcatgttgaa atcgccgtga tggtgatctt cgtcactttt ttccgcgtgg tcgtgatcat
    20641 catcatcgcc gtgaatactt ttcatcagta gcggtttcac atcttcaagc tgcgcaatcg
    20701 ttacctgctt cgcttctggt aatttgctta ccggtttttg cataaacgct tccatctccg
    20761 ggccaaccca aacgactaag tccgcgttct gtaagcgttt tacatccgat ggacgcagtg
    20821 aataatcatg ttctgaagcg ccgtcaggca gtaaaacctc cgtttctgtt acaccatcag
    20881 caatggcaga agcgatgaac ccaacgggtt taagcgaagc gacaacggcg gcatctgcgg
    20941 cctgtgttgc acctccccag agagcggcgg ataatgctgc gaaaagaagc gtttttttat
    21001 gtaacataat gcgaccaatc atcgtaatga atatgagaag tgtgatatta taacatttca
    21061 tgactactgc aagactaaaa ttaacatgac aagtctggtt tccctggaaa atgtctcggt
    21121 ttcttttggc caacgccgcg tcctctctga tgtgtcgctg gaacttaaac ctggaaaaat
    21181 tttgacttta cttgggccaa atggcgcagg taagtcgaca ctggtacggg tagtgctcgg
    21241 gctggtaaca cccgatgaag gggttatcaa gcgcaacgga aaactgcgca tcggctatgt
    21301 accgcagaag ctgtatctcg acaccacgtt gccactgacc gtaaaccgtt ttttacgctt
    21361 acgccctggt acacataaag aagatatttt gcctgcactg aaacgtgtcc aggccgggca
    21421 cctgattaac gcaccgatgc aaaagctctc tggtggcgaa acgcagcgtg tactattagc
    21481 gcgagcattg ttaaattgcc cgcaattatt agtgctggat gaacccactc aaggcgtgga
    21541 tgtaaatggc caagtggcgt tatatgacct tattgaccag ttgcgtcgtg aactggattg
    21601 tggcgtttta atggtttctc acgatctgca tctggtgatg gcaaaaaccg atgaagtgct
    21661 gtgcctgaat caccacattt gttgttccgg cacaccggaa gttgtttccc tgcatccgga
    21721 gtttatttca atgtttggtc ctcgtggtgc tgaacaactg ggtatctatc gccatcatca
    21781 taatcatcgt cacgatttac agggacgaat tgttttgcgt cggggaaatg atcgctcatg
    21841 attgaattat tatttcccgg ttggttagcc gggatcatgc tcgcctgtgc cgcgggtccg
    21901 ctgggttcgt ttgtagtctg gcgtcgtatg tcttatttcg gtgatacgct ggctcatgcc
    21961 tcattacttg gtgtcgcgtt tggtttgttg ctggacgtga atccattcta tgcggtgatt
    22021 gccgttacgc tgctgctggc gggcggtctg gtatggctgg agaagcgtcc acagctggcg
    22081 atcgacacgt tattagggat tatggcgcac agtgccctgt cgctgggcct agtggtcgtt
    22141 agtctgatgt ctaacattcg tgttgatttg atggcttacc tgttcggtga tttactggca
    22201 gtgacgccag aagatctcat ctctattgcg attggcgtgg tcatcgtggt ggctattttg
    22261 ttctggcaat ggcgcaattt gctgtcgatg acgattagcc cggatctggc gtttgttgat
    22321 ggtgtgaaat tacagcaagt gaaattgttg ttgatgctgg tgacggcatt gacgattggt
    22381 gtagcgatga aatttgtcgg tgcgttgatt attacttcgt tgctgattat tcctgcagct
    22441 actgcgcgtc gctttgcccg tacgccggaa cagatggctg gtgtcgctgt tttggtgggg
    22501 atggtggcag taactggcgg tttaaccttt tccgcgtttt acgatacgcc ggcgggtccg
    22561 tcggtggttc tatgtgcggc actgttattt attatcagta tgatgaaaaa gcaggccagc
    22621 taatctatcg ctgaacacat ttgtcggatg cggcgcgagc gccttatccg acctacggtt
    22681 cgctatctct ggctggcctg ataagacgcg aacagtgtcg catcagacac actgccagtg
    22741 tcggatgcgg cgcgagcgac caatccgact tacggcattt ctggcggcgt tatgccaaag
    22801 tgattccacg cccgcgtcgt cgccatacgc ccgcgcggtg tacgctgcaa aaagccttgc
    22861 tgaatcaaat aaggttccag tacatcttca atggtttcac gttcttcgcc aatggctgcc
    22921 gccaggttat ccagacctac cggcccacca aagaacttat cgattaccgc cagcaacaat
    22981 ttgcggtcca tataatcgaa accttcagca tcgacattca acatatccag cgcctgagca
    23041 gcgatatctg ccgagatggt gccatcgtgc ttcacttcgg cgaaatcacg cactcgacgc
    23101 agcagacggt tggcaatacg tggcgtaccg cgagcgcgac gagctacttc ctgcgcaccg
    23161 tcatcactca tctcaagccc cataaagcgt gcactgcgac tgacgatata ttgcagatcc
    23221 ggcacctgat aaaactccag acgttgcaca ataccaaaac ggtcgcgcaa cggtgatgtc
    23281 agcgaacctg cgcgcgtggt tgcgccaatc agggtaaacg gcggcaaatc aattttaatg
    23341 gagcgtgccg ccggaccttc accaatcatg atatccagtt ggtagtcttc cattgccggg
    23401 tacagcactt cttcaacaac tggcgataaa cggtggatct catcaataaa cagcacgtca
    23461 tgcggttcaa ggttagtgag catcgcagcc aaatcgcccg ccttttccag caccggacca
    23521 gaagtcgtgc gtaaattaac gcccatttca ttggcgacaa tgttggcaag cgtagtttta
    23581 cccaaccccg gaggaccaaa aatcaacaaa tgatcgaggg catcgccgcg cagtttcgct
    23641 gctttgatga aaatctccat ctgtgaacga acctgcggct gaccaacata ctcttccagt
    23701 aatttagggc gaatggcgcg atctgctaca tcttccggca aagtggtacc ggcagaaatc
    23761 agacggtctg cttcaatcat cctttacctc ataacgcggc gcgtagggct tcgcgaatta
    23821 atgtttcact gctggtgtca gggcgagcga ttttgctcac catgcggctt gcttcttgtg
    23881 gtttatagcc tagcgccacc agcgcggcaa ctgcttcttg ttcagcatcg tcggtcgccg
    23941 ggctagcagg agacgtgagt accaggtcgg cggctggcgt aaagagatcg ccatgcaaac
    24001 ctttaaatcg gtctttcatt tcaacaatca agcgttcggc ggtttttttg ccaatacccg
    24061 gcagtttcac cagtgccccc acttcttcac gctcaacggc attaacgaac tgctgcgctg
    24121 acattccgga gaggatcgcc agggccaact tcgggccgac gccgttggtt ttgatcaact
    24181 ctttgaacaa cgtgcgttct tgtttattgt taaaaccgta cagcagttgc gcgtcttcac
    24241 gcaccacaaa gtgggtgaaa acgatcgctt cctgacccgc ttcagggagt tcataaaaac
    24301 aggtcatcgg catatgcact tcatagccta cgccgcccac ttcaattaac accagcgggg
    24361 gttgtttttc aatgatgatg cctctgagtc tgcctatcac atgacgctcc tgcgtaatga
    24421 atcaaagata atgctgtatg ataaaaaaat gctggataga tatccagcga aggatgaaga
    24481 aaacttgcga ggtgtcccga tgatctgaaa aatggcacag tataatttat tcaacaggtt
    24541 atattggaag caaatatttg aatattacat attcagtgaa gaaatgcgta ataaaaatat
    24601 acattgcgac tcctgaaaaa taattttttt atgctattac gtatatttgt atctatttca
    24661 atggaatgac aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt
    24721 tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa
    24781 tcacgtcggg atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt
    24841 tcccctttca accatcacca cgctatctcg ttttattaaa cgttcgtcta atcaacgcta
    24901 tgctataaag cgattagacg ctgggctgac agaacaacaa aaacaacgaa ttgttgaaca
    24961 ggttccctcc cggctacgca aactttacca cacgggtttt aaatacgaat cttcgcgcca
    25021 gttctgttca aaatttgtct ttgatattta taaagaggcg ctatgtattc cggtgggtga
    25081 aatagagacg tttggacaac tgttaaatag caatccgaat gcaaaactca ctttctggaa
    25141 attctggttc ttaggttcta ttccgtggga gcgtaaaacc gtcacgccag ccagtttgtg
    25201 gcatcatccg ggtttggtgt tgattcacgc ggagggagtt gaaacgcctc agccggaact
    25261 gaccgaggcg gtataactta acgcagtcgc cctctcgtca ggttcagccg cgattcgctc
    25321 atctgcatcg cattctgact aacgtggcag tgggtgatgg caatcgccag cgcatcggcg
    25381 gcatccgcct gtggattagc gggcagtttc agcaaggtgc ggaccatatg ctgcacctgg
    25441 cttttttcgg cactacccat acctaccacc gtttgcttta cctgacgtgc cgcgtattca
    25501 aataccggca attcctgatt caccgccgca acaatcgcca cgccgcgcgc ctggcccagt
    25561 ttcagggctg agtcagcgtt cttcgccata aagacttgtt caatggcgaa ataatcaggc
    25621 tggaactggg tgatgatttc cgtcacgccc gcatagatga gcttcagacg agacggtaaa
    25681 tcatccactt tggtgcgtat gcatccgcta cccaggtagg acagttgcct acctacctgg
    25741 cggatgacgc cgtagccggt cacgcgcgaa cccgggtcaa tgccgagaat aatagccatc
    25801 acgcgtctcc gttttgctgt ttagcaggca tcatcagaga gtcgctgcaa cctcatcaga
    25861 gatttcaccg ttatggtaaa cttcctgcac gtcgtcgcaa tcttccagca tatcgatcag
    25921 acgcatcagt ttcggcgcgg tttctgcatc catatcagct ttggtggacg ggatcatgga
    25981 aacttccgcg ctgtctgctt tcagacctgc cgcttccaga gcgtcgcgca ctttacccat
    26041 ttcttcccat gcagtgtaga catcaatcgc gccgtcatca tatgtcacaa cgtcttcagc
    26101 acctgcttcc agagctgctt ccatgatggt gtcttcgtcg cctttctcga aggagatcac
    26161 gccttttttg ctgaacagat aagctacgga accgtcagtg ccgaggttac cgccacattt
    26221 gctgaatgcg tgacgcactt cagcaacggt acggttgcgg ttgtcagaca gacattcaat
    26281 catgattgcc gtgccgccag gaccgtaacc ttcgtagatg atggtttcca tgtttgcatc
    26341 atcatcaccg cccacaccac gtgcaattgc gcggttcagt gtgtcacggg tcatgttgtt
    26401 agacagtgct ttatccaccg ccgcacgcag acgcgggtta gcgtccggat cgccaccgcc
    26461 cagcttagcc gcggttacca gctcacgaat gattttagtg aagattttac cgcgcttagc
    26521 atcctgcgca gctttacgat gtctggtgtt ggcccattta ctatgacctg ccataaaaat
    26581 atctccagat agccctgcct gttcaggcag cgttaattac aaactgttca atcgcctgcc
    26641 ggttgctcca ggacttagtg agcgccgccg cagcaggcgc atcaagccac ttgtaagcca
    26701 gatgttcagt gaaaacgatc tgccgctcgt gcggaagcgc aagacagaac catgattccg
    26761 tattacgcgt cacgcccggc gcatagcgat gacgtaaatg tgaaaaaatt tcaaactcta
    26821 ccgtgcgctg acagtcaatt aaggtcagtt gttcagcgac aacatcaatg gtaacctctt
    26881 cctttacttc gcgcatggca gcttgcggcg cggtttcacc ctcttccacg ctgccggtta
    26941 ccgactgcca gaaatcggga tcgtcacgcc gctgcaacat cagcacccgt ttcgtatctt
    27001 gtgcatagat gaccactaag atcgaaacgg gacgcttata cactttatcc ttcacgctgc
    27061 ctcttcgttg actgccttcg ctcatcccat tcacatagtt atctatgctc atgggagttc
    27121 actcagttgc cgccttgatg caacgcgaat gatttcgtgt atttgagtca tatcagttat
    27181 tctcagcctt cttcacaacc tgaatgctca gctcagccag tgcagtcggg ttagcaaagc
    27241 tcggtgcttc agtcatcaga cacgctgccg ccgtggtttt cgggaaggcg ataacgtcac
    27301 ggatattgtc ggtgccagtc agcagcatgg tcagacggtc aagaccgaat gccagacctg
    27361 cgtgcggcgg agtaccgtat ttcagggcgt cgagcaggaa gccgaatttc tcgcgctgtt
    27421 cctcttcgtt gatacccaga ataccaaaca ccgtctgctg catatcacca ttatggatac
    27481 gtactgaacc accgcccact tcgtaaccat tgatgaccat atcgtaagcg ttcgccaccg
    27541 cattttccgg tgcagctttc agttctgcag cagtcatatc tttcggtgag gtgaacggat
    27601 ggtgcattgc cgtcaggccg ccttcaccgt cgtcttcaaa catcgggaag tcgataaccc
    27661 acagcggtgc ccatttgctt tcgtcggtca gaccaaggtc tttacccact ttcaggagca
    27721 gtgcgcccat cccgtcggca acaattttct tgttgtcggc accgaagaaa atcatatcgc
    27781 catcttgcgc ggcagtacga tccaggatgg cttcgatgat ttctgcatta aggaacttcg
    27841 ctaccgggct gttgatacct tccagacctt tcgcgcgttc gttaactttg atgtaagcca
    27901 gacctttcgc gccgtagatt ttaacgaagt taccgtattc gtcgatctgc ttacgggtca
    27961 gcgatgcgcc acccggaaca cgtagagcgg caacacggcc tttcggatcg ttcgctggcc
    28021 ccgcaaatac tgcaaactca acagatttca gcagatcggc aacgtcggtc agttccatcg
    28081 ggttacgcag atccggttta tcagaaccat aacggcgttc tgcttccgca aaggtcatta
    28141 ccgggaaatc gcccagatcc acgcccttca cttccagcca caaatgacgc accagcgctt
    28201 ccatcacttc acgcacttgc ggcgcggtca tgaaagaagt ttccacatcg atctgagtaa
    28261 attcaggctg acgatcagcg cgcaggtctt cgtcacggaa gcatttaacg atctgatagt
    28321 aacggtcaaa accggacatc atcagcagct gtttgaacaa ctgtggggat tgcggcagtg
    28381 cgtagaattt acctttgtgc acacgagaag gcaccaggta gtcacgcgcg ccttccggcg
    28441 tggctttggt cagcatcgga gtttcgatgt cgaggaagcc gtggtcatcc ataaaacggc
    28501 gcaccaggct ggtgatttta gcgcgggttt tcaggcgttg cgccatttcc gggcgacgca
    28561 ggtcgaggta gcggtatttc agacgcgctt cttcggtgtt gacgtggtta gagtcaagcg
    28621 gcagaacatc tgcgcggttg atgatagtca gcgaggacgc cagcacttca atttcgccgg
    28681 tcgccatatc gcggttaata tttttttcgt cacgcgcacg tacggtgccc gtaacctgaa
    28741 tgcagaactc attacgcagt tcagaggcca gctttaacgc gtccgcacga tccggatcga
    28801 aaaatacctg cacgatacct tcgcggtcgc gcatatcgat gaagatcagg ctaccaagat
    28861 cacgacgacg gttgacccaa ccacacagag tcacctgctg ccccacgtgg gacaaacgga
    28921 gctgtccaca atattctgta cgcatgagat atcccttaac ttagctgccg gcggatgccc
    28981 cctgctgcgc aggtgaccaa gtcgcagcgt tagctgtatg tcacaactga atgaaaaaag
    29041 gcggctatta tactggaaat tctgccgcac cgtaagagcc tggcccgcgc tggaacgcct
    29101 cgttaccact ttatatcggg cctgaaacca gactctacgc cagtttgcta taaaggtgtt
    29161 gcccgaactc ataaaaatta acaaaatttg tcgttccgcc atcggctaat cgcattaagg
    29221 tgtgaggcac gattttgttt tttcaggagt cagcatgctt gaacttaatg ctaaaaccac
    29281 tgcgttggtg gtgatcgatt tacaagaagg catcttacct tttgccggtg gtccacatac
    29341 tgccgatgag gtagttaatc gcgccgggaa gctggcggcg aaatttcgcg ccagcggtca
    29401 gcccgtgttt ctggtgcgag ttggctggtc tgccgattac gccgaagcat taaaacagcc
    29461 ggttgatgc
//
LOCUS       DAZSNO010000036        29248 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZSNO010000036 DAZSNO010000000
VERSION     DAZSNO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29248
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.036"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="ACHWN2_001549"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="ACHWN2_001549"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR8931133.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="ACHWN2_001550"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="ACHWN2_001550"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR8931134.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHWN2_001551"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHWN2_001551"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR8931135.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHWN2_001552"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHWN2_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR8931136.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHWN2_001553"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHWN2_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR8931137.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHWN2_001554"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHWN2_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR8931138.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHWN2_001555"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHWN2_001555"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR8931139.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="ACHWN2_001556"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="ACHWN2_001556"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR8931140.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="ACHWN2_001557"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="ACHWN2_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR8931141.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHWN2_001558"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHWN2_001558"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR8931142.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHWN2_001559"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHWN2_001559"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR8931143.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="ACHWN2_001560"
     CDS             8212..9075
                     /locus_tag="ACHWN2_001560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR8931144.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="ACHWN2_001561"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="ACHWN2_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR8931145.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="ACHWN2_001562"
     CDS             10411..11028
                     /locus_tag="ACHWN2_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR8931146.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHWN2_001563"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHWN2_001563"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR8931147.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="ACHWN2_001564"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="ACHWN2_001564"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR8931148.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHWN2_001565"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHWN2_001565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR8931149.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="ACHWN2_001566"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="ACHWN2_001566"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR8931150.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMGVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="ACHWN2_001567"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="ACHWN2_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR8931151.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="ACHWN2_001568"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="ACHWN2_001568"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR8931152.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHWN2_001569"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHWN2_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR8931153.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="ACHWN2_001570"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="ACHWN2_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR8931154.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="ACHWN2_001571"
     CDS             21859..23568
                     /locus_tag="ACHWN2_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR8931155.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHWN2_001572"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHWN2_001572"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR8931156.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="ACHWN2_001573"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="ACHWN2_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="ACHWN2_001574"
     tRNA            27625..27719
                     /locus_tag="ACHWN2_001574"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="ACHWN2_001575"
     CDS             27991..29202
                     /locus_tag="ACHWN2_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8931157.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
BASE COUNT         7229 a         7833 c         7341 g         6845 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatgggtgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttat
//
LOCUS       DAZSNO010000037        28249 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZSNO010000037 DAZSNO010000000
VERSION     DAZSNO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28249
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.037"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..389)
                     /locus_tag="ACHWN2_001576"
     CDS             complement(<1..389)
                     /locus_tag="ACHWN2_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR8931158.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVP"
     gene            complement(460..2574)
                     /gene="fusA"
                     /locus_tag="ACHWN2_001577"
     CDS             complement(460..2574)
                     /gene="fusA"
                     /locus_tag="ACHWN2_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR8931159.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2671..3141)
                     /gene="rpsG"
                     /locus_tag="ACHWN2_001578"
     CDS             complement(2671..3141)
                     /gene="rpsG"
                     /locus_tag="ACHWN2_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR8931160.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3238..3612)
                     /gene="rpsL"
                     /locus_tag="ACHWN2_001579"
     CDS             complement(3238..3612)
                     /gene="rpsL"
                     /locus_tag="ACHWN2_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR8931161.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3738..4025)
                     /gene="tusB"
                     /locus_tag="ACHWN2_001580"
     CDS             complement(3738..4025)
                     /gene="tusB"
                     /locus_tag="ACHWN2_001580"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR8931162.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4033..4392)
                     /gene="tusC"
                     /locus_tag="ACHWN2_001581"
     CDS             complement(4033..4392)
                     /gene="tusC"
                     /locus_tag="ACHWN2_001581"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR8931163.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4392..4778)
                     /gene="tusD"
                     /locus_tag="ACHWN2_001582"
     CDS             complement(4392..4778)
                     /gene="tusD"
                     /locus_tag="ACHWN2_001582"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR8931164.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4778..5500)
                     /locus_tag="ACHWN2_001583"
     CDS             complement(4778..5500)
                     /locus_tag="ACHWN2_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8931165.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5667..6479)
                     /gene="fkpA"
                     /locus_tag="ACHWN2_001584"
     CDS             complement(5667..6479)
                     /gene="fkpA"
                     /locus_tag="ACHWN2_001584"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR8931166.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6700..6918
                     /locus_tag="ACHWN2_001585"
     CDS             6700..6918
                     /locus_tag="ACHWN2_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR8931167.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6967..7557)
                     /gene="slyD"
                     /locus_tag="ACHWN2_001586"
     CDS             complement(6967..7557)
                     /gene="slyD"
                     /locus_tag="ACHWN2_001586"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR8931168.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7652..7852)
                     /locus_tag="ACHWN2_001587"
     CDS             complement(7652..7852)
                     /locus_tag="ACHWN2_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR8931169.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7862..9599)
                     /gene="kefB"
                     /locus_tag="ACHWN2_001588"
                     /pseudo
     CDS             complement(7862..9599)
                     /gene="kefB"
                     /locus_tag="ACHWN2_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9599..10150)
                     /gene="kefG"
                     /locus_tag="ACHWN2_001589"
     CDS             complement(9599..10150)
                     /gene="kefG"
                     /locus_tag="ACHWN2_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR8931170.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10281..12194
                     /locus_tag="ACHWN2_001590"
     CDS             10281..12194
                     /locus_tag="ACHWN2_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8931171.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12194..13216
                     /locus_tag="ACHWN2_001591"
     CDS             12194..13216
                     /locus_tag="ACHWN2_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR8931172.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13210..13428
                     /locus_tag="ACHWN2_001592"
     CDS             13210..13428
                     /locus_tag="ACHWN2_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR8931173.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13482..14351
                     /locus_tag="ACHWN2_001593"
     CDS             13482..14351
                     /locus_tag="ACHWN2_001593"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR8931174.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14406..14810)
                     /locus_tag="ACHWN2_001594"
     CDS             complement(14406..14810)
                     /locus_tag="ACHWN2_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR8931175.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15112..15744
                     /gene="crp"
                     /locus_tag="ACHWN2_001595"
     CDS             15112..15744
                     /gene="crp"
                     /locus_tag="ACHWN2_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR8931176.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15795..17885
                     /locus_tag="ACHWN2_001596"
     CDS             15795..17885
                     /locus_tag="ACHWN2_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR8931177.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17952..19172)
                     /gene="argD"
                     /locus_tag="ACHWN2_001597"
                     /gene_synonym="dapC"
     CDS             complement(17952..19172)
                     /gene="argD"
                     /locus_tag="ACHWN2_001597"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR8931178.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19258..19821)
                     /gene="pabA"
                     /locus_tag="ACHWN2_001598"
     CDS             complement(19258..19821)
                     /gene="pabA"
                     /locus_tag="ACHWN2_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR8931179.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19853..20455)
                     /locus_tag="ACHWN2_001599"
     CDS             complement(19853..20455)
                     /locus_tag="ACHWN2_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR8931180.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20445..20612)
                     /locus_tag="ACHWN2_001600"
     CDS             complement(20445..20612)
                     /locus_tag="ACHWN2_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR8931181.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20717..21298)
                     /gene="ppiA"
                     /locus_tag="ACHWN2_001601"
                     /gene_synonym="rotA"
     CDS             complement(20717..21298)
                     /gene="ppiA"
                     /locus_tag="ACHWN2_001601"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR8931182.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21569..22750
                     /gene="tsgA"
                     /locus_tag="ACHWN2_001602"
     CDS             21569..22750
                     /gene="tsgA"
                     /locus_tag="ACHWN2_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR8931183.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23012..25555
                     /gene="nirB"
                     /locus_tag="ACHWN2_001603"
     CDS             23012..25555
                     /gene="nirB"
                     /locus_tag="ACHWN2_001603"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR8931184.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25552..25878
                     /gene="nirD"
                     /locus_tag="ACHWN2_001604"
     CDS             25552..25878
                     /gene="nirD"
                     /locus_tag="ACHWN2_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR8931185.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26004..26810
                     /gene="nirC"
                     /locus_tag="ACHWN2_001605"
     CDS             26004..26810
                     /gene="nirC"
                     /locus_tag="ACHWN2_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR8931186.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26829..28202
                     /gene="cysG"
                     /locus_tag="ACHWN2_001606"
     CDS             26829..28202
                     /gene="cysG"
                     /locus_tag="ACHWN2_001606"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR8931187.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6834 a         7563 c         7144 g         6708 t
ORIGIN      
        1 tacggaacgc ctacctgacg acccagcagg atgtgctcac gagtctgcgg catcgggccg
       61 tcagtcgcag caactaccag gatcgcgccg tccatctgag cagcaccggt gatcatgttt
      121 ttaacatagt cggcgtgccc cgggcagtct acgtgtgcgt agtgacgggt cggggtgtca
      181 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
      241 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      301 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      361 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      421 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      481 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      541 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      601 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      661 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      721 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      781 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      841 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      901 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      961 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
     1021 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
     1081 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
     1141 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1201 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1261 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1321 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1381 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1441 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1501 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1561 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1621 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1681 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1741 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1801 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1861 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1921 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1981 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     2041 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     2101 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     2161 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2221 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2281 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2341 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2401 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2461 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2521 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2581 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2641 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2701 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2761 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2821 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2881 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2941 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     3001 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     3061 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     3121 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3181 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3241 agccttagga cgcttcacgc catacttgga acgagcctgc ttacgatctt taacgccgga
     3301 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3361 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3421 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3481 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3541 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3601 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3661 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3721 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3781 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3841 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3901 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3961 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     4021 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     4081 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     4141 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4201 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4261 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4321 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4381 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4441 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4501 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4561 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4621 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4681 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4741 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4801 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4861 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4921 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4981 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     5041 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     5101 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     5161 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5221 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5281 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5341 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5401 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa agggcgttga tccagtaaat
     5461 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5521 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5581 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5641 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5701 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5761 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5821 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5881 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5941 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     6001 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     6061 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     6121 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6181 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6241 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6301 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6361 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6421 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6481 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6541 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6601 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6661 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6721 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6781 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6841 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6901 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6961 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     7021 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     7081 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     7141 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7201 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7261 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7321 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7381 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7441 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7501 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7561 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7621 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7681 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7741 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7801 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7861 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7921 aaatctcttc cagttcgcgt cgggtttccc tggcgcgaga aatttgtacg gtatcagcat
     7981 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     8041 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     8101 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     8161 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8221 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8281 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8341 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8401 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8461 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8521 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8581 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8641 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8701 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8761 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8821 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8881 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8941 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     9001 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     9061 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     9121 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9181 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9241 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9301 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9361 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9421 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9481 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9541 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9601 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9661 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9721 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9781 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9841 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9901 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9961 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
    10021 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
    10081 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
    10141 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10201 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10261 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10321 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10381 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10441 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10501 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10561 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10621 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10681 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10741 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10801 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10861 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10921 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10981 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    11041 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    11101 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    11161 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11221 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11281 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11341 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11401 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11461 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11521 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11581 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11641 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11701 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11761 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11821 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11881 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11941 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    12001 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    12061 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    12121 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12181 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12241 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12301 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12361 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12421 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12481 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12541 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12601 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12661 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12721 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12781 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12841 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12901 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12961 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    13021 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    13081 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    13141 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13201 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13261 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13321 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13381 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13441 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13501 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13561 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13621 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13681 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13741 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13801 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13861 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13921 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13981 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    14041 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    14101 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    14161 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14221 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14281 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14341 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14401 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14461 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14521 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14581 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14641 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14701 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14761 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14821 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14881 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14941 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    15001 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    15061 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    15121 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15181 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15241 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15301 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15361 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15421 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15481 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15541 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15601 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15661 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15721 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15781 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15841 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15901 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15961 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    16021 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    16081 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    16141 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16201 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16261 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16321 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16381 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16441 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16501 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16561 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16621 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16681 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16741 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16801 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16861 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16921 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16981 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    17041 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    17101 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    17161 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17221 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17281 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17341 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17401 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17461 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17521 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17581 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17641 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17701 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17761 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17821 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17881 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17941 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    18001 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    18061 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    18121 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18181 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18241 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18301 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18361 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18421 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18481 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18541 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18601 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18661 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18721 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18781 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18841 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18901 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18961 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    19021 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    19081 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    19141 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19201 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19261 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19321 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19381 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19441 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19501 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19561 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19621 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19681 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19741 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19801 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19861 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19921 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19981 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    20041 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    20101 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    20161 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20221 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20281 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20341 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20401 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20461 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20521 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20581 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20641 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20701 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20761 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20821 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20881 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20941 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    21001 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    21061 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    21121 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21181 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21241 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21301 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21361 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21421 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21481 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21541 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21601 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21661 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21721 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21781 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21841 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21901 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21961 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    22021 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    22081 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    22141 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22201 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22261 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22321 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22381 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22441 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22501 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22561 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22621 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22681 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22741 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22801 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22861 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22921 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22981 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    23041 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    23101 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    23161 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23221 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23281 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23341 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23401 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23461 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23521 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23581 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23641 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23701 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23761 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23821 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23881 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23941 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    24001 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    24061 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    24121 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24181 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24241 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24301 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24361 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24421 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24481 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24541 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24601 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24661 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24721 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24781 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24841 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24901 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24961 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    25021 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    25081 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    25141 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25201 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25261 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25321 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25381 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25441 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25501 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25561 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25621 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25681 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25741 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25801 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25861 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25921 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25981 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    26041 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    26101 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    26161 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26221 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26281 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26341 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26401 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26461 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26521 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26581 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26641 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26701 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26761 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26821 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26881 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26941 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    27001 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    27061 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    27121 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27181 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27241 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27301 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27361 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27421 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27481 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27541 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27601 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27661 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27721 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27781 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27841 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27901 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27961 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    28021 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    28081 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    28141 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28201 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttat
//
LOCUS       DAZSNO010000038        28190 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZSNO010000038 DAZSNO010000000
VERSION     DAZSNO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28190
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.038"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHWN2_001607"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHWN2_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR8931188.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="ACHWN2_001608"
     CDS             complement(2138..2584)
                     /locus_tag="ACHWN2_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR8931189.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="ACHWN2_001609"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="ACHWN2_001609"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR8931190.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHWN2_001610"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHWN2_001610"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR8931191.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="ACHWN2_001611"
     CDS             complement(5347..6009)
                     /locus_tag="ACHWN2_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR8931192.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="ACHWN2_001612"
     CDS             6182..6595
                     /locus_tag="ACHWN2_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR8931193.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHWN2_001613"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHWN2_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR8931194.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHWN2_001614"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHWN2_001614"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFR8931195.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="ACHWN2_001615"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="ACHWN2_001615"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR8931196.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHWN2_001616"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHWN2_001616"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR8931197.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHWN2_001617"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHWN2_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR8931198.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="ACHWN2_001618"
     tRNA            complement(9861..9948)
                     /locus_tag="ACHWN2_001618"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHWN2_001619"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHWN2_001619"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR8931199.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHWN2_001620"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHWN2_001620"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR8931200.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHWN2_001621"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHWN2_001621"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFR8931201.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHWN2_001622"
     CDS             14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHWN2_001622"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR8931202.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHWN2_001623"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHWN2_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR8931203.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="ACHWN2_001624"
     CDS             14984..15841
                     /locus_tag="ACHWN2_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR8931204.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="ACHWN2_001625"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="ACHWN2_001625"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR8931205.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="ACHWN2_001626"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="ACHWN2_001626"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR8931206.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHWN2_001627"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHWN2_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR8931207.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="ACHWN2_001628"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="ACHWN2_001628"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR8931208.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHWN2_001629"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHWN2_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHWN2_001630"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHWN2_001630"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR8931209.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="ACHWN2_001631"
     CDS             complement(22884..24023)
                     /locus_tag="ACHWN2_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR8931210.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="ACHWN2_001632"
     CDS             complement(24069..26165)
                     /locus_tag="ACHWN2_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR8931211.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="ACHWN2_001633"
     CDS             complement(26165..26911)
                     /locus_tag="ACHWN2_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR8931212.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHWN2_001634"
     CDS             complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHWN2_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR8931213.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="ACHWN2_001635"
     CDS             complement(27659..27964)
                     /locus_tag="ACHWN2_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931214.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT         6779 a         7190 c         7451 g         6770 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagacaaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa
//
LOCUS       DAZSNO010000039        27878 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZSNO010000039 DAZSNO010000000
VERSION     DAZSNO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27878
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.039"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..240
                     /locus_tag="ACHWN2_001636"
     CDS             <1..240
                     /locus_tag="ACHWN2_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931215.1"
                     /translation="KKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEM
                     FWNLQGDLNLTCWFHYKDGQWRVITYIFWPPGARF"
     gene            265..2478
                     /locus_tag="ACHWN2_001637"
     CDS             265..2478
                     /locus_tag="ACHWN2_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFR8931216.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            2501..2946
                     /locus_tag="ACHWN2_001638"
                     /pseudo
     CDS             2501..2946
                     /locus_tag="ACHWN2_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            2968..6526
                     /locus_tag="ACHWN2_001639"
                     /pseudo
     CDS             2968..6526
                     /locus_tag="ACHWN2_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            6746..6949
                     /locus_tag="ACHWN2_001640"
     CDS             6746..6949
                     /locus_tag="ACHWN2_001640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931217.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            6967..7287
                     /locus_tag="ACHWN2_001641"
     CDS             6967..7287
                     /locus_tag="ACHWN2_001641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931218.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(7353..7585)
                     /locus_tag="ACHWN2_001642"
                     /pseudo
     CDS             complement(7353..7585)
                     /locus_tag="ACHWN2_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(7695..8465)
                     /gene="yafV"
                     /locus_tag="ACHWN2_001643"
     CDS             complement(7695..8465)
                     /gene="yafV"
                     /locus_tag="ACHWN2_001643"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR8931219.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            8646..9092
                     /gene="ivy"
                     /locus_tag="ACHWN2_001644"
     CDS             8646..9092
                     /gene="ivy"
                     /locus_tag="ACHWN2_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR8931220.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9135..11579)
                     /gene="fadE"
                     /locus_tag="ACHWN2_001645"
     CDS             complement(9135..11579)
                     /gene="fadE"
                     /locus_tag="ACHWN2_001645"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR8931221.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            11819..12397
                     /gene="lpcA"
                     /locus_tag="ACHWN2_001646"
     CDS             11819..12397
                     /gene="lpcA"
                     /locus_tag="ACHWN2_001646"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR8931222.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            12603..13370
                     /locus_tag="ACHWN2_001647"
     CDS             12603..13370
                     /locus_tag="ACHWN2_001647"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR8931223.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(13341..14081)
                     /gene="dpaA"
                     /locus_tag="ACHWN2_001648"
     CDS             complement(13341..14081)
                     /gene="dpaA"
                     /locus_tag="ACHWN2_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR8931224.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            14465..14986
                     /locus_tag="ACHWN2_001649"
                     /pseudo
     CDS             14465..14986
                     /locus_tag="ACHWN2_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15162..15659
                     /gene="rayT"
                     /locus_tag="ACHWN2_001650"
     CDS             15162..15659
                     /gene="rayT"
                     /locus_tag="ACHWN2_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR8931225.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(15876..17588)
                     /locus_tag="ACHWN2_001651"
                     /pseudo
     CDS             complement(15876..17588)
                     /locus_tag="ACHWN2_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            17560..18345
                     /gene="lafU"
                     /locus_tag="ACHWN2_001652"
     CDS             17560..18345
                     /gene="lafU"
                     /locus_tag="ACHWN2_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR8931226.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            18416..19471
                     /gene="dinB"
                     /locus_tag="ACHWN2_001653"
     CDS             18416..19471
                     /gene="dinB"
                     /locus_tag="ACHWN2_001653"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR8931227.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            19523..19816
                     /gene="yafN"
                     /locus_tag="ACHWN2_001654"
     CDS             19523..19816
                     /gene="yafN"
                     /locus_tag="ACHWN2_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFR8931228.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            19819..20217
                     /gene="yafO"
                     /locus_tag="ACHWN2_001655"
     CDS             19819..20217
                     /gene="yafO"
                     /locus_tag="ACHWN2_001655"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFR8931229.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            20227..20679
                     /locus_tag="ACHWN2_001656"
     CDS             20227..20679
                     /locus_tag="ACHWN2_001656"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8931230.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            20925..21968
                     /locus_tag="ACHWN2_001657"
     CDS             20925..21968
                     /locus_tag="ACHWN2_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR8931231.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22030..22644
                     /gene="prfH"
                     /locus_tag="ACHWN2_001658"
                     /pseudo
     CDS             22030..22644
                     /gene="prfH"
                     /locus_tag="ACHWN2_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(22701..24158)
                     /gene="pepD"
                     /locus_tag="ACHWN2_001659"
     CDS             complement(22701..24158)
                     /gene="pepD"
                     /locus_tag="ACHWN2_001659"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR8931232.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            24420..24878
                     /gene="gpt"
                     /locus_tag="ACHWN2_001660"
     CDS             24420..24878
                     /gene="gpt"
                     /locus_tag="ACHWN2_001660"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR8931233.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            24970..26214
                     /gene="frsA"
                     /locus_tag="ACHWN2_001661"
     CDS             24970..26214
                     /gene="frsA"
                     /locus_tag="ACHWN2_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR8931234.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            26272..26673
                     /gene="crl"
                     /locus_tag="ACHWN2_001662"
     CDS             26272..26673
                     /gene="crl"
                     /locus_tag="ACHWN2_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR8931235.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(26712..27767)
                     /gene="phoE"
                     /locus_tag="ACHWN2_001663"
     CDS             complement(26712..27767)
                     /gene="phoE"
                     /locus_tag="ACHWN2_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR8931236.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         6820 a         7022 c         7442 g         6594 t
ORIGIN      
        1 aagaaatatg ggccgcctgc tcatgagttc gtgaggccac gatataaatt tgttggtgag
       61 tattatatcg gcttgaatca aacttatcgc ataacagatc ctgaatttca agatgttcct
      121 attaaggaaa tgttctggaa tttgcagggg gatttgaatc tgacatgttg gtttcattat
      181 aaagacgggc aatggagagt gattacttat attttttggc caccaggggc tagattttaa
      241 tccggtatta aaggagcaaa tatcatgagt tttgtatcta cgggtaataa accagttgga
      301 aacggtggac ccgtcatcac cacaccacca atagctggcg aaagtggcgg tatgagcaca
      361 ggttcagctg taaccgatgt atctggtgcc gccgaggaga tggcagaaca ggctgcggcg
      421 gacctgtttg gtgcgctgcc ggaaccgtca ggtctggtta aagccgcagt agctgctgcg
      481 caggctgccg ctgcagcagc agggatctcg gatatggctg gtgcagttca ggatgcagcg
      541 gcgtcgcttg cagccggtgc tccgggggcg cataacgtca cagtaagtgg tagtgcggtg
      601 ccgccgcaga tgcttttgtt tgcaggcatg aatggttcgg aagggcttgg gaatctgttt
      661 tcttacacag tacagcttaa aacgctggac gccctgaacc tggggtacgt ttcccccgct
      721 gctaacctgc cgcttcagct catggtgggc aaagacctgt gcgtcagtat cgaactggac
      781 ggcggcggaa aacggtatat cagcgggctg gtgacggcgg cgagggtggt cggccatgag
      841 agccgttcgg taacgtatga attacgcatt gagccatggc tgaagctgct gacccatacc
      901 agcgactaca aagcgttcca gaacaaaacc gtggtggata ttctcgatga agtactggat
      961 gaatattcct ttccggtgga aaagcggctg gtggaaaatt atccaccccg cgcctggcag
     1021 gtgcagtatg gtgaaaccga cttcgatttc atccagcgcc tgatgcagga atgggggatt
     1081 tactggtggt ttgagcacag cgagaacagc cacacgctgg tgctggtgga tgccatcaat
     1141 ggtcataaag cgtgccctga ctcgccgctg gtggagtggc accaggaagg gctgaaactg
     1201 gataaggaat ttatccacac cattacggcc agtgagaggc tgcgtactgg aaagtgggtg
     1261 atggacgatt ttgattttat gaagcctcgt tcgctgctta aaagtaccgt ggccagtccg
     1321 cgtgataccg gtcatgctga atacgagcat tacgaatggc ctggtgatta cttcactacg
     1381 ggcgaaggcg agatgctgac gcgcattcgt atggaagcgc agcgcagccc aggcagccgg
     1441 gcacatgggg ccggtcatat ccgtaccctc atgaccgggt acacctttac gttgatgaat
     1501 cacccgacgg cagaaattaa tcaggaatat ctgctggtac agaccactct gtttttacgg
     1561 gataatgcgc agcacagcgg tcaggatcag cattttacct atgtcaccac gtttgaactg
     1621 caccccacca gcgaagttta ccgcccacaa cgaaccctga gcaaacccca caccaaaggc
     1681 ccgcagagcg ccatcgtcac cggcccggcg gggcaggaaa tctggacgga taaatatggc
     1741 cgggtgaagg tgcagtttgg ctgggaccgt tacggaaaga atgacgaaaa cagttcctgc
     1801 tggatccgcg tcagctatcc gtgggcgggt aaaggattcg gcatgatcca gatcccgcgt
     1861 attggccagg aagtactggt ggatttcaaa aacggcgatc cggatttgcc catcatcgtg
     1921 gggcgcacct ataaccagga caccatgccg ccgtgggggc tgccgggcat ggcgtcgcag
     1981 agtgggatat tcagccactc attgcaggga gggccgacta acggaaatat gctgcgcttc
     2041 gatgataaaa cgggtgcgga agaggtgaag ttccatgcgg aaaaagacct caataccacg
     2101 gtaaaaaaca atgaaaccca cacggtcatg gtcgatcgca taaaaaccat tgtcaaaaat
     2161 gaaaccatca gcgtgggaga agaccgtaat gccaccatca caaaaaatga tggtctttcc
     2221 gtcaaactgg ctcaaaccat caatgtcgga acgacatatc gtctggatgt tggcgatcaa
     2281 tttacccttc gttgcggcaa tgcagcgctt gtattacaca aggatggctc gattgagttt
     2341 tgcggaaaac aactgttact gcacaccagc gatgttatgc aactcattgg cagtggcatt
     2401 gatatgaatc cggatggcgg cacagccatg actgcggatg atattgcccc gttacctacc
     2461 cccgacgttt ctgaataaaa aatataaaag gaatttttct atgaccagcc tttaccgtat
     2521 tcaggagggc tgctttgccc tgcctgaaac ttttcttgat cgcaccgtca atatcttcgt
     2581 cccatcgggc aatgaaaggg caactcccag tctgaatatt ttccgcgata cgctcaggcc
     2641 agatgaaaat ctgaccacct atattgaccg ccagatagcc ttgatgaaaa aaaaaaccta
     2701 ggccagcacc gggtactttc gcggggaacg gcactggcgg gaactggcga agctgccatg
     2761 acaggtgaac agattctcgc aactcatcgt tctggaaaaa cagaagtgta ccagcgtcag
     2821 gccgggttta tcaccgggcc agcaaaggta ctaatgttga cgctgaccac tcagcgtcct
     2881 tttgatgatc acaccgacca gctatggact gcatggttga ctagttttca gcctgctaaa
     2941 agctaaaaaa aacagggagg ttttgtcatg tatgaagccg cacgggtgga tgaccccatc
     3001 taccacacca gcgcccttgc cgggtttttg ataggggcca tcataggtat cgccattatt
     3061 gccgtcgcca gctttgcttt ttttacctgc ggctttttgg ccgggctgat tttgagtttt
     3121 ctggctgatc agatagcttc cggagtcctg caactgggtg aggctattgg gcgctcgata
     3181 cattccacag tgggaaaaat tctgaccggt tctgataatg tcagcaccaa cagtcgcccg
     3241 gccgcccggg ccgtactgag tacggtgata tgcgaagacc attccccgga aatccgcatt
     3301 gcccaaggtt ccggcaacat ctacatcaac agccagctgc ccgtaaggat gaccacactg
     3361 agtgtgatgc agtgattgag gacggctccc ccaatgtctt tctcggcggc ggtacgcaga
     3421 cggtactgga aatcagcccg gaaatccctg actggctgcg tcaggtggtg gatgtgctgt
     3481 atgtggtcgc cggaatgctt ggcgggctgg ctggcgcctg gcgtcaggcg gcaaagatgg
     3541 ggtcgaaatt tggtacaaaa tgtgcggcaa aatttattgg tggcgaaatg gtcgggatgg
     3601 ccatctctga cacagtcatg ggattgttca gcaatccggt agatgtcacc actgggcaaa
     3661 aaattttgct gccggagacg gattttaccc tgccaggacg cctgcctgtc acctgttcgc
     3721 gcttttacgc cagccacctg gaaactgaag gattgctggg cagaggatgg cggctgaact
     3781 gggagataaa cctgcgtgag gatgaaacat atatcacctt catcggtgta cagggtcggg
     3841 aactgagtta tccgaaagag atgctgatac cgggccacca gatattcgac ccggaagagc
     3901 agttttacct gagccgtctg catgacggat gctatgtgct gcattacacc gactgcagct
     3961 attacgtgtt tgatgaattt gatgaccacg gcgtcgcgcc gttacttttt atggagacgc
     4021 cgtaccggca gcgtattgct ttcgggcgtg agaacggcag gctggtgcgg gtggcctcca
     4081 gcagcggaca ccatctgtta ttgcaccgca cgatgacgca agcaggggaa cgcctgtcgc
     4141 atattgaact tttgaagggc gggcgtcccg gtaacctggt ggaatatcgt tacgacgata
     4201 acgggcaact gactggcgtg gtgaaccggg ctggtgtgac ggtgcgccag tttgcctatg
     4261 aaaatggcct gatgacagag catcgcaacg cgacgggctt tacctgtacc taccactggg
     4321 aagagattga aggtttcccg cgcgtggtgg aacacacgac cagcgacggc gaggattacc
     4381 ggttccacta tgattttgct ggcgggcaga cggtggtaac cggcaggccg gagcagaagt
     4441 ggcagtggtg gtttgatgaa gagacgtatg tgactgcgca ccggacgccg ggcggcggaa
     4501 tgtaccgctt cacgtacaac gaaaaccatt ttccggtagc ggtggaactc cccggcgaac
     4561 gcagggtaac gctggaatac gacaccctga gccgggtggt gaaagagacg gacccggcag
     4621 gccgggtgac acagacgcag tggaacggca gttttgctga aatcacgcgc cgggcgctgg
     4681 atgacgacca cgtatggaag gcggattaca acgaacacgg ccaggtgata cgggagacgg
     4741 acccggaagg gcgcgtcacg cggtacgggt atgatgatca ggggctgccg gagacggtct
     4801 gccaccccgg taaacagcag gaccgttaca cctggaacgc gctggggtta ctgagcagcc
     4861 accgtcgcat cacgggcagt gtgcagtcct ggcagtatac gcagcgcggg atgctggcgc
     4921 gccatacgga tgaagagaaa cgggagacgc gctggcagta caccccggaa gggctggttg
     4981 ccagtctgag caacggcaac ggggctcagt accggttcag ctacgacggc gacggacggc
     5041 tgacgggaga acagcgcccg gacggcctga taaggatgtt tgcgctgaat gcggacgggt
     5101 ttccggtcat catcccgacg cagggcacag agggcggggt gcgcaatgaa cagcaggagc
     5161 gtgatgcgct gggccgcctg ctgcgcagtg acacgcagca cagtacacgg accttcagct
     5221 acaaccgtct tgaccagata acggaagtga cgctcacccc cacggaagaa ggggaacggc
     5281 tgcaccacat gcaggcggac acggtgcgtt ttgcgtatga ccgcagcggg tggctgacgg
     5341 cagagcacag cgtgcacggc agtatcaaat accggcgcga tgcgctgggc aatcccaccg
     5401 atatcacgtt gccggacggg cagcatctca gtcatcttta ctacggcagc gggcatctgc
     5461 tccagacggc actggacggt atcacggtca gtgaatatga gcgtgacagc ctgcaccgtc
     5521 aggtgatacg cacgcagggg aaactggcga cgttcagcgg ctacaacgcc gacaaccggt
     5581 taagctggca gcgcagcctg ccaggtggca gccagaatcc acagcaggcg gtactccccc
     5641 ggcgcagaac gacagcgtga cggcgcggga ctatatctgg aacgcagacg gtgaagtcgg
     5701 cggcatcaat gacaaactgc gcggttgcct ggtgttcagc tatgaccgca gcggctggct
     5761 gaccagccgc acggggcaga tgtatgacca tgaccattat tattacgaca aagcgggcaa
     5821 cctgctgacg gatgagtatc agggagcggt gatggataac cgcctgccgg gttacgggcg
     5881 tgaccgctac cgctataacg aatggggcga actgacagag cgcagggacc agcagctgga
     5941 gtggaacgcg cagggccagt taacgcgggt catcagcagc aacagcgaga cgcggtatca
     6001 gtacgatgcg ctgggcaggc gcatcagcaa ggcgaccagc aatctccata ccgaccgggg
     6061 agaacgaagc cggacaacgt ttgtgtggga agggttccgg ctgttacagg agacaacgtg
     6121 gcagggaaag cggacatacc tgtacgatgc ggaacagccg tacacgccgg tggctgccat
     6181 cacggggcgc ggagagagcc agaaaatctg gtattaccac acggacctga cgggcacggt
     6241 acacgaggta accgcgccgg acgggacgct ggtgtgggcg ggctaccagg cgggatttgg
     6301 tgaaaaccgg ggcgacatca gcaacagcgg ggcgtacttt gagcagccgc agcggctgcc
     6361 ggggcagtac tttgacgaag aaacagggct gcattacaat ctgttcaggt attatgcgcc
     6421 ggagtgcggg aggttcgtaa gccaggaccc gataggatta aacggtgggt tgaacttata
     6481 tgcttatgcg cctaatccgt taggttggat cgatccgcta ggactgaatt ctttaggaat
     6541 agattcaaat aatatattta gaggagatgg taactataaa ggtggcggaa ttggacagcc
     6601 aaagagtggc acaatatcag cacaggacat tattgatcat ctctaagaaa aaggtgatgg
     6661 gcgtttaaca tcttttagta ctaaaaaaac atctagttcc aactctcgtg gtgcagattt
     6721 tttctcaaaa aaccatgtct tcaaagtggc agtttctgat ctcgatgatt tagttaaaaa
     6781 taatcagatc actgttttaa cacaagaaaa agtaaaagca atatttggtt tagaatcaag
     6841 caagaaaatt agaaaagaac ttggtaatat tcttcagaat atggctagga ataatgaggt
     6901 gttagttcat gggcaaattc ttggaaataa agttagccga tgtaattagt tagtggagag
     6961 gaagttgtgg acgaaattag aataagaatt gctggtaata cggctgcgcc ttgtttccat
     7021 gtgctaaaaa ataagggata taaagtaaca atggaaactc atgcattatc tgacgattgt
     7081 ttagattgtg tatatgattg gtatgcggaa aaaaatggac aattttttag tgcaacgagt
     7141 ccagaagagt tacttggatt agttgcaatg tgggaagttc gtggtaatga ctggagagct
     7201 aatttgaatg agataaatac ttatcaaaaa gccatagaag aatccccgtt ttttgataaa
     7261 aacggcaatg aaatagagtc tgactaaatt ttattgatat agcaacgata ataaccggaa
     7321 atttcttcca gatctttccg gctttttatt atttaacttt cgttcagcac actaaacatc
     7381 ccgtctcgca tccccttaac tggctgttta acctgataaa gttccctgcg cagttcctgt
     7441 acttcgttgt tatcccaagc aggatcaaac atccctccga gatcttcacg gtaacgcccg
     7501 tgccgatctc aaatcccgct tttctcagcc atttggcgct gattttgatc accggcgtgt
     7561 cgctgcggtt gggcagataa cccacggtac agcgacgctg tgttttggaa atggtgactt
     7621 ctggcgtaat attgtgttta agttaggggc tttcgtaatc tagcccagcc cccggcccta
     7681 aacacaaccc ccactcacca caacctaaac tcatccgcat cccgccatgc cggaaacttt
     7741 tctctatatt cccgcaatgc caccatcgac agctccgcat caatacgcgt tgccttatgc
     7801 gcgtcggcgg tagcgataat ctcgccttgc ggattaatca cccggctgtc accgcgataa
     7861 tggcagccgt tgccatcgct gccgacgcga ttgcatcccg ccacatacgc ctgattctca
     7921 atcgcgcggg ccgtcagcaa tgcctgccag tgcagagagc gcggagctgg ccagttggcg
     7981 acgtacaggg cgaggtcata atcgttgaga ttgcgcgacc acactggaaa acgtaagtcg
     8041 tagcacacca gcggcaaaat acgccagccg cgccattcca caatcactcg cgcattgccc
     8101 gctttataat gcagatgctc atctgccatg cggaacagat gacgcttatc ataaaaatgt
     8161 accgtgccgc ccggctcaac cagcaaaaag cggttaaccg aaccagactc cgtttgtaat
     8221 gcaacactgc ctgcaatcag cgcattgcac tgctgcgcct tagctgtcat ccagttcact
     8281 acgtcatctt gtgcaagcga cgaagctgcc gcttccatgg caaagccgct ggtaaacatc
     8341 tccggtagaa cgatcacatc gcgcccggta ataccttcca gttgacgatc aaaatggcgc
     8401 aggttggcag gaccatccac ccacaccaac ggttgctgca aaagcgtaat cttcaaacta
     8461 ggcacggtgt acaactcctt tatgcgaagg gttttataac tttaacacct tatcaggcag
     8521 ttgccttagc gcagaataaa ttgataacaa atgctgatat tggaaatatc tgatttgcaa
     8581 attatcgtgt tatcgccagg ctttaggagg ttaataacat gggcaggata agctcgggag
     8641 gaatgatgtt taaggcaata acgacagtcg ccgcactggt catcgccacc agtgcaatgg
     8701 cgcaggatga tttaaccatt agcagccttg caaagggcga aaccaccaaa gctgcgttta
     8761 atcagatggt gcaagggcat aagctacctg catgggtgat gaaaggcggt acttatactc
     8821 ccgcacaaac cgtaacgttg ggagatgaga cgtatcaggt gatgagcgcg tgcaaaccgc
     8881 atgactgtgg ctcgcaacgt atcgctgtga tgtggtccga gaaatctaat cagatgacgg
     8941 ggctgttctc ggctattgat gagaaaacgt cgcaagagaa actcacctgg ctgaatgtga
     9001 acgatgcgct ttcgattgat ggtaaaacgg tgctgttcgc ggcgttgacc ggcagcctgg
     9061 aaaaccatcc ggatggcttt aattttaaat aattagcgga taaagaaacg gagcctttcg
     9121 gctccgttat tcaattacgc ggcttcaact ttccgcactt tctccggcaa ctttaccggc
     9181 ttcgtcgcca gctcttccgg atcaaagtca tcaacgttaa tactgcgcag acggctttct
     9241 tccgctttca ccagaatagc ggcttcatct ttatcaatca gccccttcgt cagcgcgttg
     9301 tgcgccagtt catccagacg ggtaaatggc agatttttac ccagctcttt acagatccgc
     9361 tgatgaatcg ggtcggcggc aatcacatcc accaacgcct cttccagtaa accaaccggg
     9421 ttatgctcgc ttggcgtcag gtactgaccg cgaccaatac gtgaacgagt ggcgttcggc
     9481 acttgtaaaa tcttcgccat tttatgatcc agcttgtcag acggtgccag ataatgacgt
     9541 ccggtcggga agatcaccac attcagcagc ccggcaacca cgcggttcgg gaagttttgc
     9601 agtaaattgt ccattgcctg ttctgcctga tacagcgcat cctgtacgcc ccagtgcacc
     9661 agcggcagat cggcttcatt acggccttcg tcgtcataac gcttcagaac ggcagaggcg
     9721 aggtaaagct ggcttaaaat atcccccaga cgtgccgaga tacgctcacg acgtttcagg
     9781 ctgccgccca gcagtgccat cgagacatca gaaagcaggg cgaggttggc gctcaggcgg
     9841 ttcaggtgct gatagtagcg tttagtggca tcgccggttg gcgtgctgct ggttaaaccg
     9901 cgcgtcaggc ccagccagaa gctgcgaact ttgttgctac cgacgtgacc gatatgtttg
     9961 aacaacagtt tatcgaacgc gttgacgtca ttgttcttcg ccgcttccat ctcttccagc
    10021 acgtacggat ggcaacgaat cgctccttgt ccgaagatca tcatgctgcg ggtcagaatg
    10081 ttagcccctt caacggtgat ggcaatcggt gcgccctggt aagcacgcgc caggaagttg
    10141 ctttgcccga gcataatgcc tttaccgccg gtaatatcca tcgcatcaat aatcgactgc
    10201 tgcccgcggt gggtacagtg atacttaacg atagccgaca gcacggcagg tttttcgccg
    10261 agcataatgc cgtaggtaat cagcgatgcc gcagcatcca ttacataagc gttcccggca
    10321 atacgcgcca gcggctcttc aatcccttcc atcttaccga tagagatttt gaactgacgg
    10381 cgaatgtgag aatacgcgcc cgttgccagc gctaccgatt tcacgccgcc ggttgagttg
    10441 gaaggcaggg tgatgccgcg gcctaccgag aggcactcca ccagcatccg ccagccttgc
    10501 ccggccattt tcggcccgcc gatgatgtaa tcgatcggca cgaagacatc tttaccgcgc
    10561 gtcggtccgt tctggaacgg tacgttcagc gggaagtggc gacgaccaat ttccacgccc
    10621 ggcgtggtgg ttgggatcag cgcacaggta atgcctaaat cttctgcacc gccgagtaat
    10681 ttttccgggt cggagagttt aaacgccagc ccaagcacgg tcgcaatcgg tgccagcgta
    10741 atgtagcgtt tgttccaggt cagacgcatc cccagcacct gctggccctg ccattcgccc
    10801 atgcagacaa tcccggtgtc cggaatcgcg cccgcatcgg aacccgcttc cgggctggtc
    10861 agtgcaaagc aggggatctc ctgaccacgc gccagacgcg gcagatagtg atctttctgc
    10921 tcgtcagtgc cgtaatgttg caacagttcg cccgggccta atgagtttgg cacgccgacg
    10981 gtaatcgcca ggatcccgct cacgccagag agtttttgca gcacacgaga ctgggcataa
    11041 gctgagaact ccagtccgcc gtactctttt ttgataatca tcgcgaagaa gcgatgctct
    11101 ttaaggtacg cccacaactc cggtggcaga tccgccagct catgagtaat ctggaaatca
    11161 ttcgccatcc ggcaggcttc ttctaccggg ccgtcgagaa acgcttgctc ttcagcggtc
    11221 aggcgcggct gcggatagtt atgcagcttt ttccagtccg gcttgccctg gaacaagtcg
    11281 ccctcccacc aggtggtgcc cgcatcaatc gcttctttct cagtgcgcga catcggcggc
    11341 atcaccttac ggaaaccgcg aaataccggc gcggaaatca tcgacttacg cataggcgca
    11401 aagttaaatg gcacgaggat aatggtcaga ggcaccagta cccacgccga ccacagacca
    11461 gcaacgccga gggcggctgt ccaggcgagc aaaatcagac tgctgataaa taagctcacg
    11521 cggtgataga acaacgcgcc gagcaggaca accgtagcga gaatactcaa aatcatcata
    11581 acgaaaagcc ccttacttgt aggaggtctg accacttgtg atgatatggt tgtagtggat
    11641 gtaaaaacat ttagcaatgt gtttacaata taattacaac aaagctcaca ttgttgctgt
    11701 ttttatccgc acttcaggtc aaaaagtcct ggtcatagca cctgcccgta cttctcgctt
    11761 ttggcggtat ccggtacact gcattttgtc tattacattt atgctgaagg atatcctcat
    11821 gtaccaggat cttattcgta acgaactgaa cgaagcggcg gaaacgctgg ctaacttttt
    11881 aaaagatgac gccaatattc acgccattca gcgcgcggcg gtcctgttag cagacagctt
    11941 taaagccggt ggcaaagtgc tttcctgcgg caacggcggt tcccattgcg acgctatgca
    12001 ctttgccgaa gagttgaccg gtcgctaccg tgaaaaccgt ccgggctacc cggcgattgc
    12061 tatttctgac gttagtcata tttcctgcgt cggtaatgat ttcggtttca atgatatttt
    12121 ctcccgctac gttgaagcgg taggtcgcga aggcgatgta ctgctgggga tctccacctc
    12181 cggtaactct gcaaacgtga tcaaagcgat cgcagcggcg cgtgagaagg ggatgaaagt
    12241 gatcaccctg accggtaaag acggcggtaa aatggctggc acggcggata tcgaaattcg
    12301 cgttccgcac tttggttatg ccgaccgcat tcaggagatt cacattaaag tgatccatat
    12361 cctgatccag ttaattgaaa aagagatggt taagtaagtc tggcgtaggc cggataaggc
    12421 gtttacgccg catccggcac ttgtgcgctg atgcctgatg cgatgctgac gcgtcttatc
    12481 atgcctacaa atctgtaccc gaaccgtagg ccgaataatt cgtttacgcc gcatccgacc
    12541 tgaaaattct taaatcaatc ttcgccgggg tccatgcgct cccgctgttg tggaggttac
    12601 ccatgtgcga attgctcggg atgagcgcca acgtccctac cgatatctgc tttagtttca
    12661 ccggacttgt acagcgtggt ggtggaaccg ggccacataa agatggctgg ggcattacct
    12721 tttacgaagg taaaggctgt cgcacattta aagatccaca acccagcttt aattccccca
    12781 tcgccaaact tgtccaggac tacccgataa aatcctgttc ggtggtggct catattcgcc
    12841 aggctaatcg gggcgaggtg gcgctggaaa atactcaccc atttacccgc gagttatggg
    12901 ggcgtaactg gacttatgcc cataacggac aactgacggg ctacaaatca ctggaaaccg
    12961 gcaacttccg cccggtcggt gaaaccgaca gcgaaaaagc cttttgctgg ctgctgcata
    13021 aattaacgca gcgttacccg cgcacgccgg gcaacatggc ggcagtgttt aaatatatcg
    13081 cctcactggc ggatgaactg cgggcgaagg gcgttttcaa catgctgctt tcggacgggc
    13141 gctatgtaat ggcgtattgt tcgactaatt tacactggat cacccgccgc gcgccgtttg
    13201 gcgtggcaac gttgctggat caggatgtgg aaatcgactt cagctcgcag accacaccga
    13261 atgatgtggt cacggtgatt gcgacacagc cgctgacggg caatgaaacc tggcaaaaaa
    13321 ttatgccggg cgaatggcgc ttattttgcc tcggggagcg tgtagtttga tgccagttgt
    13381 ggctgcacca cttcgtggct taacggcttg ctgaccacgt aacggccatt ggccacagaa
    13441 acggtcggtg gcttacgggt ttgctcaaag tagtcgtagc ccggcttcag ctgctcccag
    13501 aagtccttaa agttggaata tttatggcgc ttcatattgg cgtcggtcat gcggaatggg
    13561 taaatactca cctgcacgct cggctgacca aacaccagcg caccagtaac gaactggaag
    13621 atctcatcaa taccctgatt ggtcattgcg tagcagccga tggatacaca atcgccgtgg
    13681 atcatcaggt atttcccttc ataaccatgc gcacggtcat aagcattggg gaatccaata
    13741 ttaatcgctt tgtagtaacg gctgtctggt tttaactgat tacgctggac gctataaaac
    13801 ccttccgggc ttttgaaatc gccctgacgc tgttttggcc ctaagccgcc ggaatattta
    13861 cagattttat aactgtcgag cagttgatat tgctcgccca ttttgacgta aagatcgagc
    13921 gtacgttctt ccttgaagat ctggatgtag acaggggatc ccatcaactg ctgcttatac
    13981 tctttgctca ctggcgtggt cgagctactg ctgcccagca aaccggcaaa cgaaacgcac
    14041 gggatcaaca acatcgcaag aattaatgcg attttacgca tactgcttat tccttgataa
    14101 aacggttaca cacgccagga cggcaaaatg gatcccaaat cggaatagtc tggatttgga
    14161 aggctcacat tatcaccaaa agagttttac gcaagcctgt cggcgctggg tttacaaatt
    14221 tagcacaggg gcagttttca atagtccgaa aagcgcatgt atcatttatt tcctaatatt
    14281 atgaaatctc aatagtaata tgcaaataag caattaataa tcccgtttac tcctccggtc
    14341 ttttaaaatt ctcaccttca ttcagccggt ttatgtcttt catgtcttcg ttcttgctcg
    14401 gcagattttt gcatccaggc gtgttttcgt tatgcgtttt gctcccttta ctcgccagcg
    14461 ccaccacaac cgggagcaga taaaacagca tccagaatgg tttcctgcgc cgctcaaggc
    14521 gagtgacaga cgctggcagg cgctggcgga aaacaaccac tttttaagca gcgatcacct
    14581 gcataacatc accgaagtgg cgattcaccg cctggagcag cagcttggca agccttacgt
    14641 ctggggcggc acgcggcccg atcaaggctt cgattgtagt gggttagttt tttatgccta
    14701 caacaagatc cttgaggcga aactcccgcg cacggccaat gagatgtacc actaccgtcg
    14761 ggcaacgatt gtggcgaata acgacctgcg ccggggagat ttgctatttt tccatatcca
    14821 cagccgcgag attgccgacc atatgggggt gtatctgggc gacggtcagt ttattgagtc
    14881 actgcgcacc ggggaaacca ttcgggtaag ccggttagcc gaacctttct ggcaggacca
    14941 ttttttaggc gcgcgcagga ttttgacgga agagatgatt ttgtaggccg gataaggcgt
    15001 ttacgccgca tccggcggtt gtgcacggat gcctgatgcg acgctgacgc gtcttatcat
    15061 gcctacaaat ctgtgcgttt ttgcgagccg ctttcccgat ataacacagc gtcattgtcg
    15121 gcctttcctt ttcctcttac cgttgagaaa aaggagtcgc tatgtcggaa tatcgtcgtt
    15181 attacatcaa ggggggaaca tggtttttca cggtaaattt acgcaatcgt cgaagccatc
    15241 ttctgaccac ccagttccag acgctccgta acgccattat taacgttaag cgagacagac
    15301 cttttgaaat taacgcctgg gtcgttttgc cggagcatat gcattgcata tggacattac
    15361 ccgaaagcga tgatgatttt tcctctcgct ggcgggagat taaaaagcag ttcacccatg
    15421 cttgcggatt gaaaaatatc tggcaaccgc gtttttggga gcatgccatt cgcaatacaa
    15481 aagattatcg ccatcatgtt gattatattt atataaatcc agagaaacat ggctgggtaa
    15541 agcaggtgag tgattggcca ttctcaacgt tccatcgcga tgtcgcgcga gggttatatc
    15601 ccatcgattg ggcgggagac ataacggatt taagtgccgg ggagcgtatc attttataac
    15661 tgtgcgctgg tgccggatgc gcttacgctt atccggccta caaatcgtgc tttaacggta
    15721 ggccggataa ggcgttcacg ccgcatccgg cagttatgcg cagatgcctg atgcgacgct
    15781 ggcgcgtctt atcatgccta aaaacttgtg cctgaacggt aggccggata aggcgtttac
    15841 gccgcatccg gcaattgtgc accgatgccg gattcttaca tcaacgcccc cataatcttc
    15901 aactccagct catccggcac ttcgttatac gacagcacat gcagccccgg cgcaaacaac
    15961 cttgcataac gcggcagcaa agggcgtagc tgcggcggca ccagcagcac cggatctttc
    16021 cccgccgctt tcatctgctc tttcacctgt ggcatcgtgc tctggaactg gttgagcata
    16081 ttcgggtcaa ccggcacgct gtcgagcatc actttgccgg cctgttgcgc ctgattcact
    16141 acgttggtca gcagattttc cagctcatta ttcagcgtat acaccgtcag ctcctgcttg
    16201 cgaacgaacg gatgagtaat gctgcgccgc agcgccaggc gcacatcggc cgccagcaga
    16261 atatgatctt tagtcaccgt gctactggcg accagcacgg tggcgatggt gacgatatcg
    16321 cgcagggaaa cgccttcggt cagcagcgcc cggtacactt tcagcaattg gctgtaattg
    16381 agcgccgcgc ttaaatcctc cgccaggcgc ggtgccatcg acgacaaacg gttatgcaac
    16441 tgcgtgatgt catcatagtt aaacaaatca ggaatatagc tgcgcacaat cttgttcaca
    16501 tgggtggcaa tcacactggc gctgtcgatc acctgatacc ccatattcag agccttcgct
    16561 ttctgcgcag gctggatcca ggtgaccggt atcccgtacg ccgggtcgtt ccccagcacg
    16621 ccgtcaatct cgccgtaggt ttcgctggag ggcagcgcca tcagtttatc cgccggaata
    16681 tccgcttcat cagcctcaat gccgttgatg aaaatagcgt actggctggg cttgaggcgg
    16741 aagttttccc gaatgcggat ctccggcagc agcacgccgt taccgtcgga aatcacctgc
    16801 cgaacgccgc gaatccgctg ggtgagcggg ttgccctgcg ctttgtccac cagcgccacc
    16861 agcttgtagc cgaggcttaa gctgatgggc tcgatcagcg gaatagtttc ccaactgacc
    16921 tgttgttcgc tggtttcagt gatggtgcgg gtcagcgttt cgaggctttt ctcttccgcc
    16981 tccgccgcct gcgggcgttt gctcatccgc cagccggtaa agccaagcag ggcgctgaac
    17041 agcaaaaacg gcagatgcgg catccccggc accaccgcca gcacgaacat gatcccggtg
    17101 gcggtgtaga gcaccgacgg gcttgccagc aactggtggc gcacgtcatg ggcgatatcg
    17161 ccgttgtcgc tgacgcgggt gacgataatc gccgccgcgg tggagagcag cagggaaggg
    17221 atctgcgcca ccaggccgtc gccgatggtc atcagcacgt attgctggaa ggcggcgtcg
    17281 gcgctcaggt tgtatttgaa gatgccgata cagacgccgc cgctcaggtt gatcgccaga
    17341 atcatcatcc cggcgatggc gtctccgcgt acaaacttcg atgccccgtc catcgcgccg
    17401 tagaaatcgg cctcgctggc aacatcttta cgccgggttt gcgcctgcgc ctggttgatc
    17461 aatccggcgt taagatcggc gtcaatcgcc atctgtttgc cgggcatcgc gtccagggta
    17521 aagcgggcag aaacctcgga aatacgctcg gcctctttgg tgacgacgat aaagttgatt
    17581 gtcaacagcg tcagtaaatc cgagcgtgaa agcattatcg ccgcgctgca cgggcagtcg
    17641 atttttaacg gtggtgggct gtcgccgtta aataaaatca gcccgtctca tccgccaaaa
    17701 cctgccaccg tggcggtgcc ggaagagacg gagaaaaaag cgcgtgatgt gaatgagaaa
    17761 acggcgctgc tgaagaagaa atccgccacc gagcttggcg agttggcaac cagtatcaac
    17821 accattgccc gtgatgcgca tatggaagcg aatctggaga tggagattgt tccccaggga
    17881 ttacgcgtgc tgattaaaga cgaccagaat cgcaatatgt tcgaacgcgg cagcgcgcag
    17941 attatgccgt tctttaaaac tttgctggtg gagctcgcgc cagtgttcga ctcgctctat
    18001 aacaaaatta ttattaccgg gcataccgat gcgatggcct acaaaaacaa tatctacaac
    18061 aactggaacc tttcgggcga tcgcgcgctt tcagctcgtc gggtgctgga agaggccgga
    18121 atgccggaag ataaagtgat gcaggtaagc gcaatggcgg accagatgct gctggacgcg
    18181 aagaatccgc aaagcgcggg caatcggcgt attgagatta tggtgctgac caaaagtgcg
    18241 tccgatacgc tgtatcaata ctttggtcag catggggata aagtggtgca gccgctggtg
    18301 caaaagctgg ataagcagca ggtgctttcg cagcgaatgc gctaaatgct gaatctttac
    18361 gcatttctca aaacctgaaa tcactgtata ctttaccagt gttgagaggt gagcaatgcg
    18421 taaaatcatt catgtggata tggactgctt tttcgcagcg gtggagatgc gcgacaatcc
    18481 cgctctgcgc gatatcccta ttgctattgg cggcagccgc gaacgtcggg gggtgatcag
    18541 caccgccaat tatcccgcgc gtaaatttgg cgtacgtagc gctatgccta ctgggatggc
    18601 gctcaaatta tgcccacatc tcaccttgct tccggggcgc tttgacgcct acaaagaagc
    18661 ctcaaatcat atccgtgaaa tcttctcgcg ctacacctcg cgcattgaac cgttgtcact
    18721 ggatgaggct tatctcgatg tcaccgatag cgtccattgc cacggttctg cgaccctcat
    18781 cgcccaggaa atccgccaga cgatcttcaa cgagctgcaa ctgacggcat ctgcgggcgt
    18841 ggcaccagta aagtttctcg ccaaaatcgc ctccgacatg aataaaccca acggtcagtt
    18901 tgtgattacg ccggcagaag ttccggcatt tttacaaacc ttaccactgg aaaaaatccc
    18961 cggcgtcggc aaagtctcgg cggcaaaact ggaagcgatg gggctgcgga cctgcggtga
    19021 tgtacaaaag tgtgatctgg tgattctgct taaacgcttt ggcaaatttg gccgcatttt
    19081 gtgggagcgt agtcagggga ttgacgagcg cgacgttaac agcgaacggt tacgaaaatc
    19141 cgtcggcgtg gaacgcacga tggcggaaga tattcatcac tggtctgaat gtgaagcgat
    19201 tatcgagcgg ctgtatccgg aacttgaacg ccgtctggca aaggtaaaac ctgatttact
    19261 gattgctcgc cagggggtga aattaaagtt cgacgatttt cagcagacca cccaggagca
    19321 cgtctggccg cggctgaata aagctgactt aatcgccacc gcgcgtaaaa cctgggatga
    19381 acgccgcggc gggcgcggtg tgcgtctggt ggggctgcat gtgacgttgc ttgacccgca
    19441 aatggaaaga caactggtgc tgggattatg atgtatacta ttatgtatat tctggtgtgc
    19501 attattatga gggtatcact gtatgcatcg aattctcgct gaaaaatcgg tcaatatcac
    19561 tgagttacgt aaaaacccag ctaaatactt tattgatcaa ccagttgcgg ttctttctaa
    19621 taatcgcccc gcaggatatc tcttaagtgc cagcgcattc gaagcgttaa tggacatgct
    19681 tgctgaacaa gaggagaaaa agcccataaa ggcgcgcttt cgtccaagtg ctgcaagatt
    19741 agaggaaatt acacgccgcg ctaaacaata tcttaatgat atgacggatg atgatttcaa
    19801 tgactttaag gaataaggat gcgggtattc aaaacaaaac ttattcgcct gcaacttaca
    19861 gcagaggaac ttgatgcgtt aacggcggat tttatttcct ataagcgtga cggtgttttg
    19921 ccagatatat ttggtcgcga tgcactctac gacgactcgt ttacctggcc attaatcaaa
    19981 tttgagcgag ttgctcatat tcatctggca aatgtgaata atccatttcc gccacagttg
    20041 cgccaattca gcagaacgaa tgacgaagcg catttggtat attgtcaggg tgcgtttgat
    20101 gagcaagcat ggttgctcat tgccattctg aagcctgaac ctcataaact ggctcgagat
    20161 aacaaccaaa tgcataaaat tggaaaaatg gcagaagcgt ttcgcatgcg tttttgaatt
    20221 tatattatga ataacataca aataagagat tatcagcctg gcgattttca gcaactatgc
    20281 gctattttca ttagagcggt tacgatgacc gtcagtcagc attattcact acaacaaatt
    20341 gccgcctggg cgcagattga cgaatctcgc tggaaggaga aactcgcgaa atcacaagtg
    20401 cgggttgcag ttattaatgc aaaactggtc ggctttatta cctgcgttga acactatatc
    20461 gatatgttat ttgttgaccc tgaatacacc cgccgtgggg ttgccagcgc tttgttaaaa
    20521 ccttttatta agtctgaatc cgaacttacg gtggacgcaa gcataaccgc aaaacccttt
    20581 tttgaacgtt atggttttca gacagttaag cagcagcgcg ttgaatgccg gggagcgtgg
    20641 tttattaatt tttatatgcg ttataaaccg caacattaaa tccagcttgc aatgaaaata
    20701 acgcccgcct agtatgtgcg cgttttctta ttccacaaac tgcaaggagg taaatcatga
    20761 caaatccttt atcaatgact ctttgcagac ctttccagga ttaattcttt ttttcttgcc
    20821 ctggattcgt ctgccatttc ctgatttttt aattgttact ctcaataatt caagtcgcag
    20881 cacatgcaac ttgaagtatg acgagtctat ttacggaatg gtatatgggc aattatattc
    20941 gtcccttatc cgatgcggta tttaccatcg catctgatga cctgtggatc gagagtttag
    21001 cgatccaaca attacacacc acggcaaatt tacccaacat gcagcgcgta gtggggatgc
    21061 cagatttaca ccccggacgc ggctacccga ttggcgcagc gttcttctcc gcaggccgtt
    21121 tttacccagc actggtcggc aatgatatcg gctgtggcat ggcgctatgg caaacagata
    21181 ttctcgctcg caaatacaac gccgataagt ttgaaaagcg attatcagct ttggatgacg
    21241 ttgctgaaga aagctggctg gaggaaaacc tgccgtcagc atttgcacag catccgtggc
    21301 gcagctcgct tggctctatc ggtggtggca atcatttcgc tgaactgcaa caagttgatc
    21361 aaatcatcaa cgctgaactg tttgcactgg caggtctgga tgcgcagcat ctgcaactgc
    21421 tggttcatag cggctcgagg gggctagggc agtctatttt acagcggcat attgcctcgt
    21481 tttcgcatca tggtttgcct gaaggcagtg acgacgcgct ggcgtttgcg cgttttaatc
    21541 gccacctgat cgctttgcgc ataatgcaac aggttaaggc caccggaagt ccggttctgg
    21601 atgtggcgca taactttgtt agcgcgtgtc gaatcggtga tcaacaaggg gtcttacatc
    21661 gtaaaggtgc cacgcctgat gactgtggtc tggtggttat tcccggttca cgcggtgatt
    21721 actccaggct ggttcagccc gtcaggagtg aggaaacatt gcattcgctg gcgcatgggg
    21781 ctgggcgtaa atgggggcgc accgagtgta aagggcgtct ggcagcgaaa tacacagcga
    21841 cgcaactctc gcgtactgaa cttggcagtc gggtaatttg tcgcgataaa caactcatct
    21901 tcgaagaagc gccacaagcc tataaatcgg ctgaaagtgt ggtgcaatgt cagggcaggc
    21961 cggattaatt attcctgtcg cgcggctgcg tccggtgcta acgctcaaaa acagtggagg
    22021 gaaaaaagga tgatcttgct ataactctcc tcagcgcagg ggccggaaga atgttgtctc
    22081 gcagtgaaaa aggcgctgga caggctaatt aaagaagctg cccgacaaga cgtcgcggta
    22141 acggtgctgg aaacagaaac aggccgctac tctgacacgc tgcgttcggc gctgatttct
    22201 ctggatggcg acaacgcatg ggcgttaagc gaaagctggt gtggcactat tcagtggatt
    22261 tgcccgagcc cgtatcgccc acatcacggg cgtaaaaact ggtttctggg cattgggcgt
    22321 tttaccgctg atgagcagga gcaatccgat gcgatccggt atgagacgct gcgttcgtcg
    22381 gggccgggcg gtcaacatgt caataaaacc gactcggcgg tacgcgccac gcatttggca
    22441 tccggtatta gcgtgaaggt tcagtcagag cgcagccagc atgctaacaa acggctggcg
    22501 cgattgctga ttgcctggaa gctggatcag cagcaatagg aaaatagcgc ggtgctgaaa
    22561 tcacagcggc gaatgttcca tcaccagatt gaacgtggca acccgcgacg aacgtttaca
    22621 gggatggctt ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca
    22681 tccggcagca aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagcagt
    22741 gtccagtaat gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg
    22801 atagttggcc cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat
    22861 tccaggcccg cgtggataat ctggatgttc ggcgtcttgt tgaacaggcg ctgataggtt
    22921 tcacgtacca gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct
    22981 ttcgcttcgg ttttcgcgcc agccagttta cccagcgaat ccagcatgct caccacgtag
    23041 tctttaccgc tgtcgattag tgaacggatc aggcagtgaa tttctacgtt attgtcagtc
    23101 atggtcacca caccgacgtt cagggaggtt tcgaccacac cttttgccac gtcggaattg
    23161 cggatcacac cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca
    23221 atcagggcag ctttatcgtt cgctacagag tccagcaaca aggccagatt tttctctttc
    23281 tctgccagct cgtttttcag gatctcctga taggtattca ccagagattt cagtgcgtcg
    23341 actttatcag ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt
    23401 gtgccgccgt tgaaatcgat aaggcgcagg tccagttctt ctgcatgacc cgccaggaag
    23461 cgcaccagca gtttgttggc attacccagg ccaacgtgga tttcaccgcc ggagtgaccg
    23521 cctttcagac cttttaaggt taacttgaag gtttcaaaac cagctggaac cgcttcacga
    23581 tctaaatgca ggttggaggt gaagtcgata ccccccgcac aacccatgta gatttcacct
    23641 tcttcttcgg agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagccg
    23701 aacgcaccgt ccataccggc ttcttcggtc atggtcagca gcacttccag cgggccgtga
    23761 accacgtttt cgtcagccag aaccgccagt gcagaggcca taccaatgcc gttatccgca
    23821 cccagcgtgg tgccgcgcgc tttaacccat tcgccatcaa tataaggctg gataggatct
    23881 ttcgtgaagt catgcacggt gtcgttattt ttctgcggca ccatatcgag gtgggcctgt
    23941 aagacgaccg gtttacgatt ttccatacct gcggtagcag gttgacgaat caggatatta
    24001 cctacctgat cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca
    24061 gcgagttgct cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc
    24121 cacagcggct gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac
    24181 ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc
    24241 ttgcgagata tgtattgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
    24301 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
    24361 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
    24421 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
    24481 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgg
    24541 taccgggggc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
    24601 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
    24661 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgct gttgcgattc
    24721 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
    24781 tggttgatga ctatgttgtt gatatcccgc aagatacctg gatcgaacag ccgtgggata
    24841 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcattgc
    24901 cgggcgttgt tctttttaac ttcaggcagg ttacaatagt ttccagtaag tattctggag
    24961 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
    25021 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
    25081 gctcttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
    25141 tggcgcggta ttgacccacg tgaaatcctc gacgtccagg cacgtattgt gatgagcgat
    25201 gctgaacgta ccgacgatga tttatacgat accgtgattg gctaccgtgg tggcaactgg
    25261 atttatgagt gggccaccca ggcgatggtg tggcaacaaa aagcctgtac ggaagaagat
    25321 ccgcaactca gtggtcgaca ctggctgcat gcggctacgt tgtacaacat tgccgcctat
    25381 cctcatctga aaggagatga cctggccgag caagcgcagg ctttatcaaa ccgcgcctat
    25441 gaagaggccg cccagcgtct gccgggcacg atgcggcaga tggagtttac cgtacccggc
    25501 ggtgcgccca tcaccggctt tttgcatatg ccgaaaggcg atggcccgtt cccgacagta
    25561 ttaatgtgtg gtggtctgga tgcgatgcag acggactatt acagcctgta tgaacgttat
    25621 tttgcgccgc gcggcattgc gatgctgact attgatatgc cgtcggtggg cttttcttca
    25681 aaatggaagc tcacccagga ctccagcctg ttgcatcagc acgtcttaaa ggcgctgcct
    25741 aacgtaccgt gggtggatca cactcgcgtc gcggcctttg gtttccgttt cggcgctaac
    25801 gttgccgtgc gtctggcata ccttgaatcg ccgcgtctga aagcggttgc ctgtcttggt
    25861 ccggtagttc ataccctgtt gagtgatttt aagtgccagc aacaggtgcc ggaaatgtat
    25921 cttgacgttc tggcgagtcg tttggggatg catgatgctt ccgacgaagc gttacgcgtg
    25981 gagctgaatc gctattcatt aaaagtgcaa ggattgctgg ggcgtcgctg cccaacgcca
    26041 atgttatcag gctactggaa gaacgatccg ttcagcccgg aagaggactc acgcttaatc
    26101 acctcatcat ctgctgacgg taaattatta gagatcccat ttaacccggt gtatcggaat
    26161 tttgacaaag gtcttcagga aatcaccggc tggatcgaaa aacgcttgtg ttaaaaattt
    26221 gctaaatttt gccaatttgg taaaacagtt gcatcacaac aggagatagc aatgacgtta
    26281 ccgagtggac acccgaagag cagattgatc aaaaaattta ccgcactagg cccgtatatt
    26341 cgtgaaggta agtgcgaaga taatcgattc tttttcgatt gtctggctgt atgcgtcaac
    26401 gtgaaaccgg caccggaagt gcgtgaattc tggggctggt ggatggagct ggaagcgcag
    26461 gaatcccgtt ttacctacag ctaccagttt ggtctgttcg ataaagcagg cgactggaag
    26521 agtgttccgg taaaagatac tgaagtggtt gaacgactgg agcacaccct gcgtgagttt
    26581 cacgagaagc tgcgtgaact gctgacgacg ctgaatctga agctggaacc ggcgaatgat
    26641 tttcgtgatg aaccggtgaa gttaacggcg tgagtgaaat gtgccggatg catcacatcc
    26701 ggcaatattc attaaaactg atacgtcatc ccaaccgcga cagtatcatc attattaata
    26761 ttcagcttat tatcgctatc cagttggttg attttataat caacaaacgc tgacatattt
    26821 ttgttgaaat aatacgtagc accgacgtcg atataattga ccagatcttc atcaccgatg
    26881 ccctcaatat ctttcccttt cgataagaca taacccagcg atggacgcag accaaagtca
    26941 aactggtatt gagcgaccgc ttcaaagttc tgtgtcttat tggcaaagcc gccagttatt
    27001 ggcgtcattt tgcgtgtttc agaatagaaa gttgccagat aaatattatt ggcatcgtat
    27061 ttcagacctg ttgcccatgc ttctgcacgc ttgcctgtgc cacggctttg caggttctgc
    27121 tcgttggtgc gatctgagtt ggtataggcc ccactaatgg cgaaatcgct gccgccaaag
    27181 tcatatgtca atgacgtgcc gaagccatcg ccgttttgct ttttaacgtc gcggttttcg
    27241 tttttccctt gatattgcag ggttaagttc aggccatcga taacgccgaa gaagtcggtg
    27301 ttccgatacg tcgccagacc gctggcgcgt ttggtcataa agttgtcggt ctgcgccgag
    27361 gaatcgccac caaattccgg gaacatatcg gtccaggctt ccacgtcata caacgcgccc
    27421 aggttacgac cataatcgaa agaacccaaa tctttatatt tcaacccggc aaaagcgaga
    27481 cgcgtttttt gctgtgcagt atcactctcc gctttatttc cggcaaactc cgcttcccac
    27541 cgaccataac cagtcagttg atcgttaatt tgagtttcac ctttaaaacc aaaacggata
    27601 taactctggt cgccatcttt actggcgtta tcactcatat aatgcatggc tttaactttg
    27661 ccatagacat ccagtttatt accgtcttta ttatatattt ctgcagcctg tacagatgca
    27721 gatgccacaa tgcccatcac cactaatgcc agagtgctct ttttcatttt cattcctgat
    27781 tttaattaac gcgcgaatat tcagcgggag agccccgttg aaaacaggaa agtttttaac
    27841 ctgagattgt taaagatata ttacagatta atgatatt
//
LOCUS       DAZSNO010000040        27601 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZSNO010000040 DAZSNO010000000
VERSION     DAZSNO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.040"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACHWN2_001664"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACHWN2_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR8931237.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACHWN2_001665"
     CDS             complement(1614..1937)
                     /locus_tag="ACHWN2_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR8931238.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHWN2_001666"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHWN2_001666"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR8931239.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHWN2_001667"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHWN2_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR8931240.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHWN2_001668"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHWN2_001668"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR8931241.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHWN2_001669"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHWN2_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR8931242.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACHWN2_001670"
     CDS             7537..8574
                     /locus_tag="ACHWN2_001670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR8931243.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENSCQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHWN2_001671"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHWN2_001671"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR8931244.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACHWN2_001672"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACHWN2_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR8931245.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHWN2_001673"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHWN2_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR8931246.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="ACHWN2_001674"
     CDS             10745..11626
                     /locus_tag="ACHWN2_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8931247.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHWN2_001675"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHWN2_001675"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR8931248.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELHPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="ACHWN2_001676"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="ACHWN2_001676"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR8931249.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHWN2_001677"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHWN2_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="ACHWN2_001678"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="ACHWN2_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR8931250.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHWN2_001679"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHWN2_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR8931251.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="ACHWN2_001680"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="ACHWN2_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR8931252.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="ACHWN2_001681"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="ACHWN2_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR8931253.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="ACHWN2_001682"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="ACHWN2_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR8931254.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="ACHWN2_001683"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="ACHWN2_001683"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR8931255.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="ACHWN2_001684"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="ACHWN2_001684"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR8931256.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="ACHWN2_001685"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="ACHWN2_001685"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR8931257.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="ACHWN2_001686"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="ACHWN2_001686"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR8931258.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="ACHWN2_001687"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="ACHWN2_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR8931259.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHWN2_001688"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHWN2_001688"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR8931260.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /gene="acpS"
                     /locus_tag="ACHWN2_001689"
     CDS             24893..25273
                     /gene="acpS"
                     /locus_tag="ACHWN2_001689"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR8931261.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="ACHWN2_001690"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="ACHWN2_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR8931262.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHWN2_001691"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHWN2_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR8931263.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="ACHWN2_001692"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="ACHWN2_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6808 a         6863 c         7235 g         6695 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaactc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggtgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaactgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAZSNO010000041        27361 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZSNO010000041 DAZSNO010000000
VERSION     DAZSNO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27361
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.041"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..574
                     /locus_tag="ACHWN2_001693"
     CDS             <1..574
                     /locus_tag="ACHWN2_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8931264.1"
                     /translation="AEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLCM
                     DLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLDN
                     LKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKG
                     SDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="ACHWN2_001694"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="ACHWN2_001694"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFR8931265.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="ACHWN2_001695"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="ACHWN2_001695"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR8931266.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="ACHWN2_001696"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="ACHWN2_001696"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR8931267.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="ACHWN2_001697"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="ACHWN2_001697"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8931268.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="ACHWN2_001698"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="ACHWN2_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR8931269.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="ACHWN2_001699"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="ACHWN2_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR8931270.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="ACHWN2_001700"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="ACHWN2_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR8931271.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="ACHWN2_001701"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="ACHWN2_001701"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR8931272.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="ACHWN2_001702"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="ACHWN2_001702"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR8931273.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="ACHWN2_001703"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="ACHWN2_001703"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR8931274.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="ACHWN2_001704"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="ACHWN2_001704"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR8931275.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="ACHWN2_001705"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="ACHWN2_001705"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR8931276.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="ACHWN2_001706"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="ACHWN2_001706"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR8931277.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="ACHWN2_001707"
     CDS             13934..14314
                     /locus_tag="ACHWN2_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR8931278.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="ACHWN2_001708"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="ACHWN2_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR8931279.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="ACHWN2_001709"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="ACHWN2_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR8931280.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="ACHWN2_001710"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="ACHWN2_001710"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR8931281.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDAFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="ACHWN2_001711"
     tRNA            complement(17415..17490)
                     /locus_tag="ACHWN2_001711"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="ACHWN2_001712"
     tRNA            complement(17494..17569)
                     /locus_tag="ACHWN2_001712"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="ACHWN2_001713"
     tRNA            complement(17621..17696)
                     /locus_tag="ACHWN2_001713"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
     gene            complement(17699..17774)
                     /locus_tag="ACHWN2_001714"
     tRNA            complement(17699..17774)
                     /locus_tag="ACHWN2_001714"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17739..17741),aa:Val,seq:tac)
     gene            complement(17910..17985)
                     /locus_tag="ACHWN2_001715"
     tRNA            complement(17910..17985)
                     /locus_tag="ACHWN2_001715"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17950..17952),aa:Lys,seq:ttt)
     gene            complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="ACHWN2_001716"
     CDS             complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="ACHWN2_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR8931282.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18951..19472)
                     /gene="pal"
                     /locus_tag="ACHWN2_001717"
     CDS             complement(18951..19472)
                     /gene="pal"
                     /locus_tag="ACHWN2_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR8931283.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="ACHWN2_001718"
     CDS             complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="ACHWN2_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR8931284.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20932..22173)
                     /gene="tolA"
                     /locus_tag="ACHWN2_001719"
     CDS             complement(20932..22173)
                     /gene="tolA"
                     /locus_tag="ACHWN2_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR8931285.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22238..22666)
                     /gene="tolR"
                     /locus_tag="ACHWN2_001720"
     CDS             complement(22238..22666)
                     /gene="tolR"
                     /locus_tag="ACHWN2_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR8931286.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22670..23362)
                     /gene="tolQ"
                     /locus_tag="ACHWN2_001721"
     CDS             complement(22670..23362)
                     /gene="tolQ"
                     /locus_tag="ACHWN2_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR8931287.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23359..23763)
                     /gene="ybgC"
                     /locus_tag="ACHWN2_001722"
     CDS             complement(23359..23763)
                     /gene="ybgC"
                     /locus_tag="ACHWN2_001722"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR8931288.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23913..24206)
                     /gene="ybgE"
                     /locus_tag="ACHWN2_001723"
     CDS             complement(23913..24206)
                     /gene="ybgE"
                     /locus_tag="ACHWN2_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR8931289.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24206..24319)
                     /gene="cydX"
                     /locus_tag="ACHWN2_001724"
     CDS             complement(24206..24319)
                     /gene="cydX"
                     /locus_tag="ACHWN2_001724"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR8931290.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24334..25473)
                     /gene="cydB"
                     /locus_tag="ACHWN2_001725"
     CDS             complement(24334..25473)
                     /gene="cydB"
                     /locus_tag="ACHWN2_001725"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR8931291.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25489..27057)
                     /gene="cydA"
                     /locus_tag="ACHWN2_001726"
     CDS             complement(25489..27057)
                     /gene="cydA"
                     /locus_tag="ACHWN2_001726"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR8931292.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         6887 a         7255 c         6704 g         6515 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa atgcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
    17761 tgagctaatc acccgacact acgctggata ctacaactaa ctatgttagt gggggtcgtg
    17821 caggatgact cgacttcgtc tcgcccttcg gcgccgttgc tgatgcaacg ttatccttca
    17881 cgttttacta ccgcttccac cttagcgatt ggtgggtcgt gcaggattcg aacctgcgac
    17941 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt
    18001 tcggtttgtt tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg
    18061 caacaaaaat ttcgatgcag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa
    18121 taatgcgact tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt
    18181 ttagcgccat cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt
    18241 gcggtgtcac ctttgtcctg catgatgacg ccgactttaa acatcgcatc agcagccttt
    18301 ggtgacttcg gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta
    18361 cccttgttgt agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca
    18421 gggtaatttt tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc
    18481 tgcaccagcg caatagctgc attgtaatcc gtgtttgcat cgccgctttt caccggcgcg
    18541 ccagcattcg cagtaccagc atcagctgtc ggcgtcgttg atgccgtcgc accgctttga
    18601 tcgctgctgg ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg
    18661 atctgctttt gccgctccac gacctgattc agttgatact gattttcctg aatctgacca
    18721 cgcagggaat caatatcgga ttgattatca gaaagttgtt gctggagttg ggttaaaagc
    18781 tggctgtgag cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag
    18841 ctgacactac tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt
    18901 aacgacagac tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc
    18961 agtaccgcac gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt
    19021 tctttaccgt aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac
    19081 atcttaacgg cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt
    19141 tcgtccgcgt gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca
    19201 tgtgcatcca gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa
    19261 acgatgttgt tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg
    19321 ttgccgttgc cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg
    19381 tcattgctgg cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc
    19441 atcagccctt tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa
    19501 ttattatcac agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag
    19561 acgcgctttg aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga
    19621 agagctgtag attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt
    19681 ggacgacaga acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc
    19741 accattggag ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt
    19801 ctgcgaacct tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac
    19861 ctgcggacga ccggcctggt cagaagtaaa tgccaggttc tggctatccg ggaaccaggt
    19921 cggttcggta ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc
    19981 cattacgtac aggttcagac taccggtttt cgacaaggcg aatgccagtt tgctgccgtc
    20041 tggcgagaat gcaggtgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc
    20101 atttgccagc gtctgaataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag
    20161 tttagaaccg tctggtgacc acgccggaga catcagcggc tgcggtgaac ggtgaacgac
    20221 aaactggttg taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt
    20281 ggtctgaaca acgtaggcaa tacgggtacg gaacgcacct ttaatgccgg tcagtttttc
    20341 aaacacttca tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta
    20401 cgagttctga gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac
    20461 attgtaagaa ccatccggat tcggagtgac ttgaccgaca actacagcgt caatgcccag
    20521 tgcggaccat gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc
    20581 gcgatctaac ggattaaatt taccgctgtt acgcaagtca gcagcaacga tgccgccaat
    20641 atcttcaggt gccgcaccag gccccgccca ctggaaagga acaacaccaa taggacgacc
    20701 ggaatctaca ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag
    20761 tatgagaaaa ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc
    20821 gatggcccac gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac
    20881 cccgtggcaa ccggtgcctg atgttgaccg tccgaacagt caacatcgcg attacggttt
    20941 gaagtccaat ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat
    21001 cttcgcaagt ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg
    21061 tttgatatcc agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc
    21121 tgcataggac gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata
    21181 gttattgata tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga
    21241 agcattgttc cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc
    21301 accgaaaata tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc
    21361 ttttgccgct gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc
    21421 tgctgcctta tcagcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc
    21481 tgccgctttc ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt
    21541 ggctttttca gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc
    21601 cgcttccgct ttcttcttca gtgccgcagc ggctacctcg gcttttttct gcgcttcggc
    21661 tgcggctttg gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc
    21721 tgcttcttcc gcagctttag catctgccgc cgctttcgct gccgccactt cagcttgctt
    21781 ctgctttaac tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc
    21841 taaccgctct ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag
    21901 ttcttcagca gcctgctgtt ccttcatctt gcgctgttca tcagaacgct tcgcgcttga
    21961 ttcctggctt tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc
    22021 gtcgatggac gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact
    22081 ccagatcagc gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt
    22141 gagcttgtcg ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg
    22201 ttacccgctc tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac
    22261 caaccgattt cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag
    22321 gcacatcttt tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg
    22381 acacttccgc caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca
    22441 cggtgtactg accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca
    22501 ccgcctgtga ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg
    22561 ctgtcgccat aaagatcagc aacagcacca gcagtacgtc cagcaacgga acaatgttga
    22621 tttcggactt gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt
    22681 tgctctcgct aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt
    22741 tgtcgtaatt cagttccagt ttgtttacgc gctggttgag acggttataa gccattaccg
    22801 ccggaattgc ggcgaacaga ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg
    22861 caaccatttg cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc
    22921 cccagaccgt accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg
    22981 gaatgtgcgt ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg
    23041 ccccttccac tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt
    23101 tgaacccgct gtaaaagatt tgttccgaac cagtcagatt atcccgtttc ccctgactct
    23161 cttgatagag gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt
    23221 cacgcgccgc tgcgttaaga atacgggttc gctggataat aatggcccag gatgcgattg
    23281 aaaaaccaat caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat
    23341 caaggatatt catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg
    23401 aggcttcatt ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt
    23461 attctcggcg ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat
    23521 ttcagtctgt atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat
    23581 tttacgtacc acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg
    23641 acgcagcatc tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac
    23701 accaccggca tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt
    23761 cactttacat cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg
    23821 cgcgattgag gtttgggaat gagggcaagt taagggcgcg taaaaattta tgggcctaga
    23881 gcggcccata attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca
    23941 atgagaacaa tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga
    24001 cgaaagccca cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc
    24061 cagatttcca gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct
    24121 gctaacagaa gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca
    24181 tacaaggtcg cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga
    24241 cgtgttcaag cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa
    24301 tccatgcgaa ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc
    24361 aatatcttct ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag
    24421 aatgatcggt accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga
    24481 agttgcatcc cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg
    24541 gaacattgcg ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa
    24601 cgcccacgcg gctttatcca tacgtgcagt caggatggtc agcagcagca gaaccacacc
    24661 cagtgccgga atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc
    24721 ttcacgaacc acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt
    24781 cacgacataa ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag
    24841 tgtcaccagc gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt
    24901 acgcatttgc agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc
    24961 cagcaggccg aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata
    25021 ttcatcaacg ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag
    25081 cggcggaacg aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc
    25141 ttcaatcttg gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag
    25201 gatcatcgcc acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa
    25261 gagtgcgccg cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga
    25321 gttaatcata attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac
    25381 ccccatgtcg aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca
    25441 ccagataaaa cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg
    25501 ctgagtagtc gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc
    25561 gaggcgtgca aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc
    25621 gcaaatcagc accattgaga agatgagatc gcctgcggtc agtgacgaat tcgccacagc
    25681 tgtcggcagc acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc
    25741 cgcttctaca gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt
    25801 tttctcgcca atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagcag
    25861 caggaagcca cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat
    25921 ggagtctttg gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata
    25981 gcgtttcagc agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac
    26041 cgtctggtcg gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt
    26101 acgaatgcgt tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac
    26161 ggaacgcgtt gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc
    26221 ctcttcctga tcaggaatgc cgaacaaagt aaaggcagca ggcgcaggtt gcgtttccca
    26281 ctcggcttca atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc
    26341 atcacccaga acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa
    26401 ggagcgttta gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag
    26461 gatgaacatc gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc
    26521 aaccgggtta agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt
    26581 gaaatcggac gcgattgggt tttgcatcca gccgttcgca accagaatcc acagtgcgga
    26641 caggtttgaa ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg
    26701 atcccaaccg aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc
    26761 ttcgattgcc agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt
    26821 agtcccgaac tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc
    26881 aaacaacttg ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta
    26941 gaccgtttcc ataatggcca ggaggaacgc cataccgagc gtcagtggca caaaaaggaa
    27001 gtggtacatc gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat
    27061 gactccttgc tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc
    27121 atgccccaat agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac
    27181 ggcgtaaaga aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt
    27241 acgccgcaaa atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg
    27301 gtgatcaatt tatagctaaa ttgccgcctt tcagccaatt tgatcgagaa caatttatct
    27361 c
//
LOCUS       DAZSNO010000042        27059 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZSNO010000042 DAZSNO010000000
VERSION     DAZSNO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27059
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.042"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..176)
                     /locus_tag="ACHWN2_001727"
     CDS             complement(<1..176)
                     /locus_tag="ACHWN2_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR8931293.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="ACHWN2_001728"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="ACHWN2_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR8931294.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="ACHWN2_001729"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="ACHWN2_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR8931295.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="ACHWN2_001730"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="ACHWN2_001730"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR8931296.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="ACHWN2_001731"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="ACHWN2_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR8931297.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="ACHWN2_001732"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="ACHWN2_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR8931298.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="ACHWN2_001733"
     CDS             complement(5180..5422)
                     /locus_tag="ACHWN2_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR8931299.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="ACHWN2_001734"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="ACHWN2_001734"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR8931300.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="ACHWN2_001735"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="ACHWN2_001735"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR8931301.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="ACHWN2_001736"
     CDS             complement(8339..9313)
                     /locus_tag="ACHWN2_001736"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR8931302.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="ACHWN2_001737"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="ACHWN2_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR8931303.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQILIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="ACHWN2_001738"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="ACHWN2_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR8931304.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="ACHWN2_001739"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="ACHWN2_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR8931305.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="ACHWN2_001740"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="ACHWN2_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR8931306.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="ACHWN2_001741"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="ACHWN2_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR8931307.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="ACHWN2_001742"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="ACHWN2_001742"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR8931308.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="ACHWN2_001743"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="ACHWN2_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR8931309.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="ACHWN2_001744"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="ACHWN2_001744"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR8931310.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="ACHWN2_001745"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="ACHWN2_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR8931311.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /gene="aaeX"
                     /locus_tag="ACHWN2_001746"
     CDS             23180..23383
                     /gene="aaeX"
                     /locus_tag="ACHWN2_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR8931312.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="ACHWN2_001747"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="ACHWN2_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR8931313.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="ACHWN2_001748"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="ACHWN2_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR8931314.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="ACHWN2_001749"
     CDS             26388..26660
                     /locus_tag="ACHWN2_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR8931315.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="ACHWN2_001750"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="ACHWN2_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR8931316.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6449 a         6788 c         7229 g         6593 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaactggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatctg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagagt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgggcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctca ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag atcctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ctggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag gacaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattatt
//
LOCUS       DAZSNO010000043        26638 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZSNO010000043 DAZSNO010000000
VERSION     DAZSNO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26638
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.043"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            229..336
                     /locus_tag="ACHWN2_001751"
     CDS             229..336
                     /locus_tag="ACHWN2_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR8931317.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            712..819
                     /locus_tag="ACHWN2_001752"
     CDS             712..819
                     /locus_tag="ACHWN2_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR8931318.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(906..2585)
                     /gene="bcsG"
                     /locus_tag="ACHWN2_001753"
     CDS             complement(906..2585)
                     /gene="bcsG"
                     /locus_tag="ACHWN2_001753"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR8931319.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2582..2773)
                     /gene="bcsF"
                     /locus_tag="ACHWN2_001754"
     CDS             complement(2582..2773)
                     /gene="bcsF"
                     /locus_tag="ACHWN2_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR8931320.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2770..4341)
                     /gene="bcsE"
                     /locus_tag="ACHWN2_001755"
     CDS             complement(2770..4341)
                     /gene="bcsE"
                     /locus_tag="ACHWN2_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR8931321.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4614..4802
                     /gene="bcsR"
                     /locus_tag="ACHWN2_001756"
     CDS             4614..4802
                     /gene="bcsR"
                     /locus_tag="ACHWN2_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR8931322.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4814..5566
                     /gene="bcsQ"
                     /locus_tag="ACHWN2_001757"
     CDS             4814..5566
                     /gene="bcsQ"
                     /locus_tag="ACHWN2_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR8931323.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5563..8180
                     /gene="bcsA"
                     /locus_tag="ACHWN2_001758"
                     /pseudo
     CDS             5563..8180
                     /gene="bcsA"
                     /locus_tag="ACHWN2_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            8191..10530
                     /gene="bcsB"
                     /locus_tag="ACHWN2_001759"
     CDS             8191..10530
                     /gene="bcsB"
                     /locus_tag="ACHWN2_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR8931324.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            10537..11643
                     /gene="bcsZ"
                     /locus_tag="ACHWN2_001760"
     CDS             10537..11643
                     /gene="bcsZ"
                     /locus_tag="ACHWN2_001760"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR8931325.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11838..12227
                     /locus_tag="ACHWN2_001761"
     CDS             11838..12227
                     /locus_tag="ACHWN2_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HFR8931326.1"
                     /translation="MISNAACKTTVWHSRQRYWKTRANGRRRQHFSGNDWRWAPAAYG
                     LLTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQVKDYAPSHFL
                     LYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            12309..14297
                     /gene="hmsP"
                     /locus_tag="ACHWN2_001762"
     CDS             12309..14297
                     /gene="hmsP"
                     /locus_tag="ACHWN2_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR8931327.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            14484..15770
                     /gene="dctA"
                     /locus_tag="ACHWN2_001763"
     CDS             14484..15770
                     /gene="dctA"
                     /locus_tag="ACHWN2_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR8931328.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15991..17487
                     /locus_tag="ACHWN2_001764"
     CDS             15991..17487
                     /locus_tag="ACHWN2_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR8931329.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(17583..18512)
                     /gene="kdgK"
                     /locus_tag="ACHWN2_001765"
     CDS             complement(17583..18512)
                     /gene="kdgK"
                     /locus_tag="ACHWN2_001765"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR8931330.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            18744..19511
                     /gene="pdeH"
                     /locus_tag="ACHWN2_001766"
     CDS             18744..19511
                     /gene="pdeH"
                     /locus_tag="ACHWN2_001766"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR8931331.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            19581..21641
                     /locus_tag="ACHWN2_001767"
     CDS             19581..21641
                     /locus_tag="ACHWN2_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR8931332.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21823..23145)
                     /locus_tag="ACHWN2_001768"
     CDS             complement(21823..23145)
                     /locus_tag="ACHWN2_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8931333.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(23556..24569)
                     /gene="yhjD"
                     /locus_tag="ACHWN2_001769"
     CDS             complement(23556..24569)
                     /gene="yhjD"
                     /locus_tag="ACHWN2_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR8931334.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(24618..25517)
                     /locus_tag="ACHWN2_001770"
     CDS             complement(24618..25517)
                     /locus_tag="ACHWN2_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8931335.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            26037..>26638
                     /locus_tag="ACHWN2_001771"
     CDS             26037..>26638
                     /locus_tag="ACHWN2_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR8931336.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6361 a         6682 c         7270 g         6325 t
ORIGIN      
        1 gtgacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aagggttccg
       61 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acatcacggg ggccattctt gaacctgaac
      181 aactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg tcatgcgggc gtcaggctgc
      361 cgtaatggca atttgcgccc ggaccaggcc gcaggggtga aactctgcgg cctttttcgt
      421 aagaaaaatg caacgattgc acgcggataa tattctgcgg aatgcgcagc acagagtgaa
      481 gacgtgacgg attccggaac agaaaaacag tcgtagaatc agcggcaagc cggaaaggtt
      541 ccggtgaggc gcaatgttgc gggggcttta tccctggtgg cattggttgc tggagagaga
      601 aaacccccgc acgttgcagg tatgcacctg acaacatcac gggggctaat cttgaaccca
      661 aacaactcaa gaatagccgc gaaacgttgg cattacaaca caggcggcta tatgacgctc
      721 gcagaactgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggcattctt
      781 gccagtatga tcgtgaactg gctgaataag cggaagtaac gtgtcatgcg ggcgtcaggc
      841 tgccgtaatg gcaatttgcg cccggatcag gccgcagagg ttaaactctg cggccttttt
      901 cgtgcttact gcgggtaagg cacccagtcg ccgccgttca ggcgaacgta cggtttatct
      961 tggtattgaa taactactgc atttgagttc tcggagaccg gtgctgtttg tggcaaccca
     1021 ctggtgagtt ttttccagtc aacattgtct tcggtgaaaa tcttgccatc aagaacgcga
     1081 accaccagat cggagatagc caggaagctg ctcggttggt caatgacaat cggtgccccc
     1141 tgatgtggtg ccttcatgcc gaagaatttc accccaacgg ggacgtctgt gatagacggg
     1201 ctagggatat cacgtaggcc agatacctgc attctgtcgc ccttcagcgc gccgccgtgt
     1261 tccggcacca cgaccaccat cactttacga cccgatttct ccagttcagt aaagaaggcg
     1321 tccagttcat caaagaattt ctgcgcccgc gctttgtaat ccgctgtttt gctgaccccc
     1381 ggataatggt tgccgtcatg cagtggaagc gtgttgtaga acgtggcact acggctgttt
     1441 ttatcttttt cggtaacgtc cagccagcgg ttaagcacgg cggtatcgtc ataaaccggc
     1501 gaaccatcaa agcccagcaa aataaccggc agatttgttt gatccatcaa ttcagtctgc
     1561 atgccgccat tttcgcgaac ttctttcaaa aaaccgccga actggccgtt atgtcccatc
     1621 atcaggtgct gggtaaagcc cagtttcgaa aggttatcaa acagatagca gtcgttattt
     1681 gccggttgat acagattagt gtgcgaagtc tgcccgcagc tggcgcgcag taaacggatc
     1741 gccgccgggc cactgtagga ggtggcggag ttaaagttct tgaaatcaat atcgaaatgc
     1801 gaccacagtg gatgcgacat caacccggcg gcttctatat ccgaccagga aagcgaacag
     1861 atgttaatca ccagtagttc aaatggctga gcatcagcgg gcagcgaaga cgggaaggtc
     1921 gatttacgtt tcgcctccgc gttatagaaa ttattcagcc aggcgttaag gttcgccgtt
     1981 gttggcggtg cagtttgtgc gggcatatca cccactaccg gtgcgccacc cgtcgccgca
     2041 acggttgccg ctgcgttacc acccgtcgtt gttacagtgg tcgtcggttg tccggctggc
     2101 cacaaggaga aacttggtcc cgccagggta agtacgttca gccatagcag tatggcaacc
     2161 acaaaaacgg taatgcgaat ccattgtgac aggaataacc aggccactaa taaaacaaaa
     2221 atggccccaa tcatctgcca gttaataaag cgtgtgacaa ggtcgattaa ataatcggta
     2281 ctgaaccccg ccacctgcga accctggctc attatgcttt ccgggccagg caaccagatg
     2341 tcatgccaga acaaagcaaa gccgatcggc agggcaatcc agtggcgcaa gcgatgcagg
     2401 ctgtaacgcg gaatgggcat cagcagaaac gcggcaaaca ccaaattgag gagcgggtgg
     2461 aagttaagat atcccgccca caacaggcca aacttaacca gaaaatagaa gttccagccg
     2521 gaaaggccgc gccagtattg ccagagggaa gaaggcatgg cggtattttg cgtaaattga
     2581 gtcatttttt ggttgccctg gctttttccg tgcggcgtaa cgtcccggcc ggtttaacat
     2641 aacgaggttt agcaaagaac aaacgtaagg tgtcgcgaat gcgtcgcaaa gagtgccgcg
     2701 ccagatagcc cagcgggaaa aatatcagtg cgcaaacgat aataatttca atgatatcgc
     2761 tgatggtcat catgatgagc gctccacagc atcatctaac agtcgcatgg gttctggtat
     2821 tcgtcgccag tggcgaccat cgtgctcggc attgatgacc ggtttagaac tttgcgttaa
     2881 aggcagcggc attccccatt gttctggggc aagcaggcgc atctgcacca gctcggcact
     2941 gatttgatca tcttcaaacc agaccatacg gtttgagaaa atgtcgccag taggcaatgg
     3001 gaaaatatga ttcaacgcgg tatccagatc gttaatccga cagaatgaga gaaacagcac
     3061 cagccgatta ccgccaatgg tcatgatatc gccggtacgg ttagggcgac atagcgtcag
     3121 ggcttgctca acgcggatac ccggtaccgg acgtagggca accagaacgc ctttaccgtg
     3181 ggcaggcaag agagggttat tcatcatgtt gttgacggca ttacagaaca catcccactt
     3241 ctggaaacca cgcagtttga gcggctgggt cattgacagc aaggtagtga tatcttccgg
     3301 tacatagcga ctaaacttct gcccttgcac gctttcgatc atcgtcagac aacgggagag
     3361 tggcgcattc cacggaataa ccatatttgc accgcaggcc aataacaaac gttcatcggt
     3421 ggcgcgcagg ctagcggtat tttcccgcac gaggattttc atcgcactac cgcgctggcg
     3481 acgcagggta tgaatgctgc gggccagtgg ctcgatttgc gcattttgct gtaaagaaaa
     3541 gaccaccgtc gccgcctgag cggtacgggc ttcattgaac aggacttcgt tattgttgaa
     3601 cagttgccag tgttccgata gcggcggcgc accttccagt acagcaacat tactcagaat
     3661 gcgtttttcg tcgctgcgtg gttggatctc cgcctcttcg ctttgaacta atgtccagat
     3721 accattttgt tgctgaacgc taagctgctg acgggcgctg accccttttt cgttgcacca
     3781 gaaggcaata tccagcaaat gttggtcacc ctgaaaacgc aaactggcaa gaccaaaaag
     3841 tgaacggtat tcctcaagca acaatgaaaa ttgtttatcg ttattatttc cgggattaat
     3901 taccaaaagc gaacaatggt ttaacctgct ccatttattc attttatcca accatgagcg
     3961 aagccgctcg gcaggaatat tttgccatgc gttatttgcg caaacaagaa taaaaaggta
     4021 attatgggga tcaatagaac actgcaaatc acgggccaaa tagtatagac ctttttcatg
     4081 atttagcatt gaaaataatt ttattttttc cggcccttga gaatcatcta attgaaagat
     4141 tttcgccgga tcgctgtcca tgctaatgac cgcgacgtgt gcggtttcag cctgggatgc
     4201 aattgtttga ttcgccagac tgatagcatc ttcatggcga tcgacgttaa accaccagac
     4261 gccgcctgct ggcatatgtc gcagctcatc ccataatgat gagataccga tagagaatac
     4321 agggtccaca atgtccctca tgtcaccatt ttgtctgatt atcagtttac tagcgaaagc
     4381 taagaaataa acctaccatt gaaattaaaa cttatcagat ttagcatgtg aatcaattta
     4441 gaaggtcaca agacgtgtga ttttagtctt ttcgttttta actttttctg ttgcgtaacg
     4501 gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac aataaaccgg cgaaatgctc
     4561 acggttagaa ttgtctcatg aatggtacgg ttatttcata gggatcaagc aaaatgaata
     4621 acaatgaacc agatactctg cctgatcccg cgataggcta tatcttccag aatgatattg
     4681 tggcgttaaa gcaggcattt tcactgcctg atattgatta tgccgatatt tcccaacgcg
     4741 aacagttggc cgcggcatta aaacgctggc cgttgctggc agagtttgcg caacaaaagt
     4801 aggggattgg tgaatggccg tactgggatt gcagggggtg cggggaggcg tggggacaac
     4861 aaccatcacc gccgcattag cctggtcatt acaaatgttg ggagaaaatg tcctggtggt
     4921 cgatgcctgc ccggacaact tgttgcgcct gtcatttaat gttgatttta cccaccgtca
     4981 gggctgggcc agagcgatgc tggatggcca ggactggcgt gacgctgggt tgcgctacac
     5041 ctcgcagctc gatttgctgc cttttggtca gttatccatt gaagaacaag aaaatccaca
     5101 gcactggcaa acccggctga gcgatatttg ctccggctta cagcaactaa aagccagcgg
     5161 gcgttaccag tggattttaa tcgacttacc gcgtgatgcc tcgcagataa cccaccagct
     5221 gctgagtttg tgcgagcact cgctggcaat cgtcaatgtg gatgccaact gccatatccg
     5281 actgcatcag caagcgctgc cggatggcgc acatattttg attaatgact tccgtattgg
     5341 cagtcaggtt caggacgata tttaccagct ttggttgcaa agccagcgcc gattactgcc
     5401 gatgctcatt catcgtgatg aagcgatggc tgaatgcctg gcggctaagc aaccagtagg
     5461 tgaatatcgc agtgatgcgc tggcggctga agagatactg acgctggcga actggtgcct
     5521 gttgaactac tccgggctga aaacgccagt cgggagtgca tcatgagtat cctgacccgg
     5581 tggttgctta tcccgccggt caacgcgcgg cttatcgggc gttatcgcga ttatcgtcgt
     5641 cacggtgcgt cggctttcag cgcgacgctc ggctgtttct ggatgatcct ggcctggatt
     5701 tttattccgc tggagcaccc gcgctggcag cgtattcgcg cagaacataa aaacctgtat
     5761 acgcatatca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca
     5821 tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt
     5881 caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
     5941 ttagccataa aacacagcat ctggatgaaa aaaaagagct cggtcatttg agtgccgggg
     6001 cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
     6061 ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag
     6121 cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc
     6181 tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc
     6241 cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc
     6301 tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga
     6361 aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca
     6421 atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga
     6481 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
     6541 gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg
     6601 cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc
     6661 gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc
     6721 agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta
     6781 aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg
     6841 acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg
     6901 gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc
     6961 gtggctatac ctccgcgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc
     7021 tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtataa atcttccgtc
     7081 tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg
     7141 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
     7201 tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc
     7261 tgccgcatat gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact
     7321 ctttctggag tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg
     7381 tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg
     7441 aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctactcaacc
     7501 tggtgggcgt tgcggtaggc atctggcgct acttctatgg cccgccaacc gagatgctca
     7561 ccgtggtcgt cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg
     7621 cggtatcggt agaaagcaaa caggtacgcc gatcgaaccg cgtggagatg acgatgcccg
     7681 cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat ttctccgacg
     7741 gtggtttggg gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc
     7801 tgttgcttaa acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg
     7861 gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc
     7921 agtgtacgtt tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata
     7981 agccgctgga aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg
     8041 cggagtttgc gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct
     8101 gggttgtatc gtttattccg cgccgcccgg agcggagcga aacggcacaa ccatcggatc
     8161 aggctttggc tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca
     8221 gtggctatgg ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa
     8281 ccactgatca atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg
     8341 caagtgatgc caggcgagca gggagctgat gcccccatcg tggcgcagaa cggtccttcg
     8401 cgtgatgtga agctgacctt tgcgcaaatc gcgccgccgc cgggcagcat ggtgctacgt
     8461 ggtattaacc cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag
     8521 gcgatgctca acttcgaata cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta
     8581 aaggtttatc tcaatgatga gctgatgggc gtgctgccag tgaccaaaga acagttgggt
     8641 aaaaaaacgc tggcgcaaat gcccattaat ccactgttta ttaccgactt caaccgtgtg
     8701 cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag caccacgctt
     8761 tggctggatg ttgggcggag cagtggactg gatctgacct atcagaccct gaatgtgaag
     8821 aatgacctgt cacacttccc ggtgccattc tttgacccgc gcgataaccg tactaacacc
     8881 ttgccgatgg tctttgcggg tgcgccggat gttgggctgc aacaagcatc tgccattgtc
     8941 gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga acttcccggt gctctataac
     9001 caattgccgg atcgcaatgc cattgtcttt gcaaccaacg acaaacggcc ggacttcctg
     9061 cgcgatcatc cggcggtaaa agccccggtg attgagatga ttaaccatcc gcagaatcct
     9121 tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa
     9181 ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa
     9241 ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc
     9301 acatttggcg aactgaaaac ctatgaagaa cagttacaat ccagcggcct tgagccagca
     9361 gcgattaacg tttcgctaaa cctgccgccg gatctctacc tgatgcgcag taccggcatt
     9421 gatatggata ttaattaccg ctacaccatg ccgccggtga aagacagttc gcggatggat
     9481 atcagcctga ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac
     9541 cgtctgctgc tgcgtattcc ggtattacaa ggtttgctgg atggcaaaac agatgtctct
     9601 attccggcgc tgaaactggg cgcgaccaac cagctgcgct tcgactttga gtatatgaac
     9661 ccgatgccag gcggttcggt agataactgt attaccttcc agccggtgca gaatcatgtg
     9721 gtgattggtg acgattccac catcgacttc tcgaagtatt accacttcat cccgatgccg
     9781 gatctacgcg cctttgctaa cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa
     9841 accatcaccg tgatgccgaa agcgcctaac gaagcacaga tggaaacgtt gctgaatact
     9901 gttggtttta tcggcgcaca gacgggcttc ccggcgatta atctgacggt gaccgatgat
     9961 ggcagcacca ttcagggcaa agatgccgac atcatgatca tcggtggtat cccggacaaa
    10021 ctgaaagacg ataagcagat cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca
    10081 ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg agagcgatcg cgcggcagaa
    10141 acccagtcaa cgctgacctc ttccggtgcg atggcggcgg tgattggctt ccagtcgccg
    10201 tataacgacc agcgcagcgt gattgcgctg ttggcagata gcccacgcgg ttatgaaatg
    10261 cttaacgatg cggtgaacga tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg
    10321 atccgcgagt ccggtatcaa cagcctacgt gttggcgacg tttattacgt aggtcatctg
    10381 ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg
    10441 gcgactatca gtgtgatatt gctggcatgg gtactgtggc gtctgctgcg aattattagt
    10501 cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggaatc
    10561 gtgacgatgc tgttgttggc tgcctttagt gttcaggcag cctgtacctg gcctgcctgg
    10621 gagcagttta aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg
    10681 cgcaaaatca ccacctccga agggcaaagt tacggtatgt tctttgccct ggcggctaac
    10741 gaccgtgcag ctttcgataa tattctcgac tggacgcaga acaatctcgc tcagggttct
    10801 ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg
    10861 ctggacagca attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg
    10921 gggcgtttgt ggaaagagca gcgttatacc gacatcggca gcgcgttgct aaaacgtatc
    10981 gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg
    11041 ggttttgctg aggataacag ctggcgtttt aaccccagct acatgccgcc gacgctggcg
    11101 cagtatttca cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacattta
    11161 ttgctggaaa ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa
    11221 ggctggcagc taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac
    11281 atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt
    11341 aaaccgatgg cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct
    11401 acggggaaag cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcctttttta
    11461 caaaaccgcg atgcgcaggc agttcagcgc cagcgcgtgg ccgataactt tcccggcagc
    11521 gatacctatt acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc
    11581 cgcttctcga caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac
    11641 taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa
    11701 ccatcagcag accctgcgga tggacagcgg caacactaat gccgtgcgcg ggctggcgaa
    11761 tatttaccgc cagcagtcgc cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag
    11821 ccagcggcgc agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca
    11881 ggcagaggta ctggaaaacc agggcaaatg ggcgcaggcg gcagcacttc agcggcaacg
    11941 actggcgctg ggccccggca gcgtatggat tacttaccga ctggcaggaa gaagctgcgc
    12001 ggcaatccaa cgatggcggc agcagtcagg gctttggcta cacggcgcgg gcattacttg
    12061 aacgacgtgt tacttccaac tggtttgttg gcacggcaat tgatatccag caggtgaaag
    12121 attacgcacc cagccatttc ctgctctacg tacgttattc cgccgccgga tggcagggtg
    12181 acatggattt accgccgcag ccgctgatac cttacgccga ctggtaagtt ttcagatagc
    12241 gcctctctta atgccgctgc gatcgggtat actcgggcgg caatctggga tttccggggg
    12301 gagacaattt gcgcgtaagt cgctcgttaa caatcaagca gatggcaatg gtggcagccg
    12361 ttgtcctggt gttcgttttt attttttgca ccgttttgct gttccatctg gtccagcaga
    12421 atcgctataa cacggctacg caactggaaa gcattgctcg ctctgtccgc gaacccttat
    12481 cttcagctat tttgaaaggc gatattcccg aagcggaagc tattcttgcc agcattaaac
    12541 cggcaggcgt ggtcagccgt gccgatgtag tgctgcctaa ccagttccag gcgctgcgta
    12601 aaagttttat tccagagcgt ccggtgccgg taatggttac tcgcctgttt gagctaccgg
    12661 ttcaaatctc gctgggcgtc tactcgctcg aacgtccggc aaatccgcag ccaatagcct
    12721 atctggtgct acaggcggat tccttccgta tgtataagtt cgtgatgagc actctctcaa
    12781 cgttagtgac catttactta cttttgtcgc ttatcctgac cgtcgccatc agctggtgca
    12841 ttaaccgcct gattttgcat ccgttacgca atattgctcg cgaacttaac gccatcccag
    12901 cccaggagct tgttggtcac caactggcat taccgcgtct gcatcaggac gatgaaatcg
    12961 gtatgttggt gcgcagttac aacctcaacc agcaattgct gcagcgccat tatgaagaac
    13021 agaacgaaaa tgcgatgcgc ttcccggtgt cggatttgcc gaacaaagcc ttgctgatgg
    13081 agatgctgga gcaggttgtc gcgcgtaaac aaaccaccgc gctgatgatc atcacctgtg
    13141 aaaccctgcg tgatactgcg ggcgtgctga aagaggcgca acgagaaatt ctgctgctga
    13201 cgctggtgga aaaactcaaa tcggtactgt cgccacgtat gatcctcgcg cagattagcg
    13261 gttatgactt tgctgtcatt gccaacggtg tacaggaacc gtggcacgca atcactttgg
    13321 gtcagcaagt actcactatc atgagcgagc gcctgccgat tgaacgtatt caactccgtc
    13381 cgcactgtag cattggcgtg gcgatgttct acggcgatct caccgccgaa cagctttaca
    13441 gtcgcgctat ttctgcggca tttaccgctc gccataaagg caagaatcag attcagttct
    13501 ttgatccgca gcagatggaa gcagcccaga agcggttgac ggaagagagc gatatcctta
    13561 atgcactgga aaatcatcag tttgcaattt ggttacagcc acaggtcgag atgaccagcg
    13621 gtaaactggt cagtgcggaa gtgttactgc gtatccagca accggatggc agttgggacc
    13681 tgccggatgg cttaatcgat cgcattgagt gctgtgggct gatggttacc gtcggtcact
    13741 gggtgctgga agagtcctgt cgattgcttg cagcctggca agagcgcggc attatgctgc
    13801 ccttgtcggt aaacctctct gcgctgcaac tgatgcaccc gaatatggtg gcggatatgc
    13861 tggaactgtt aacccgctat cgcattcagc cgggaacact gattctggaa gtgacagaaa
    13921 gccgacgtat tgacgaccct catgctgcgg tggcaatcct ccgtccgctg cgcaatgccg
    13981 gagttcgggt ggcgctggat gatttcggca tgggctacgc agggctgcgt cagctgcagc
    14041 atatgaaatc gttgccaatc gacgtactga aaatcgacaa aatgtttgtt gaaggcttgc
    14101 cggaagatag cagcatgatt gctgcaatta tcatgctggc gcagagcctg aacttacaaa
    14161 tgattgccga aggcgtggag actgaagcac aacgagactg gctggcaaaa gcgggcgttg
    14221 gtattgccca gggcttcctt tttgctcgcc cactccctat tgaaatcttc gaagagagtt
    14281 acctggaaga aaagtagcta ccccaaactg attacaaaac tttaaaaagt gctggtttgt
    14341 gcgagccagc tcaaactttt taaccttttt gtttcaatta tgatccaggt acatttctgt
    14401 gatgttgtct gggtgttatt ttaaggccgc aggtaccccc cccataacct tacaagacct
    14461 gtggttttac taaaggacac cctatgaaaa cctctctgtt taaaagcctt tactttcagg
    14521 tcctgacagc gatagccatt ggtattctcc ttggccattt ctatcctgaa ataggcgagc
    14581 aaatgaaacc gcttggcgac ggcttcgtta agctcattaa gatgatcatc gctcctgtca
    14641 tcttttgtac cgtcgtaacg ggcattgcgg gcatggaaag catgaaggcg gtcggtcgta
    14701 ccggcgcagt cgcactgctt tactttgaaa ttgtcagtac catcgcgctg attattggtc
    14761 ttatcatcgt taacgtcgtg cagcctggtg ccggaatgaa cgtcgatccg gcaacgcttg
    14821 atgcgaaagc ggtagcggtt tacgccgatc aggcgaaaga ccagggcatt gtcgccttca
    14881 ttatggatgt catcccggcg agcgtcattg gcgcatttgc cagcggtaac attctgcagg
    14941 tgctgctgtt tgccgtactg tttggttttg cgctccaccg tctgggcagc aaaggccaac
    15001 tgatttttaa cgtgatcgaa agtttctcgc aggtcatctt cggcatcatc aatatgatca
    15061 tgcgtctggc acctattggt gcgctcgggg caatggcgtt taccatcggt aaatacggcg
    15121 tcggcacact ggtgcaactg gggcagctga ttatctgttt ctacattacc tgtatcctgt
    15181 ttgtggtgct ggtattgggt tcaatcgcta aagcgactgg tttcagtatc ttcaaattta
    15241 tccgctacat ccgtgaagaa ctgctgattg tactggggac ttcatcttcc gagtcggcgc
    15301 tgccgcgtat gctcgacaag atggagaaac tcggctgccg taaatcggtg gtggggctgg
    15361 tcatcccgac aggctactcg tttaaccttg atggcacatc gatatacctg acaatggcgg
    15421 cggtgtttat cgcccaggcc actaacagtc agatggatat cgtccaccaa atcacgctgt
    15481 taatcgtgtt gctgctttct tctaaagggg cggcaggggt aacgggtagt ggctttatcg
    15541 tgctggcggc gacgctctct gcggtgggcc atttgccggt agcgggtctg gcgctgatcc
    15601 tcggtatcga ccgctttatg tcagaagctc gtgcgctaac taacctggtc ggtaacggcg
    15661 tagcgaccat tgtcgttgct aagtgggtga aagaactgga ccacaaaaaa ctggacgatg
    15721 tgctgaataa tcgtgcgccg gatggcaaaa cgcacgaatt atcctcttaa tctcaccact
    15781 tatgcccgca ctcccttccc cgagtgcggg tgtttgcgca taattgaccc ctgtgcctga
    15841 acttaccacc tgggtgagtt caggtttatt tcacttcttc cgtgacattt tcatgttctt
    15901 gcggtctaac acgaagtgtt tttaacgtca tttagactga ccgacaacgg gcagtttttt
    15961 tattaccagg atagttgatc aggggttcac atgcagggca taaaaattcg acttttagcg
    16021 ggcggtttgc tgatgatggc cactgctggc tatgtgcagg cagatgcgct ccagcctgat
    16081 ccagcatggc aacaggggac gctttccaac ggtttacagt ggcaagtgct gaccaccccc
    16141 cagcgtccca gcgatcgtgt tgaaattcgc ctgctggtta ataccggttc gctcgccgaa
    16201 agtacacaac agagcggtta cagtcacgcc atccctcgta ttgcgctaac gcaaagcggt
    16261 ggccttgacg cagcacaggc gcgttcattg tggcagcagg ggatcgaccc taaacgcccg
    16321 atgccgccgg taattgtctc ttatgacacc acgctgttta atctgagttt gcccaataac
    16381 cgtaacgatt tgctgaaaga agcgctctct tatctggcaa atgccactgg caaattgacc
    16441 atcacaccag aaaccatcaa ccacgcgctg caaagtcagg acatggtggc aacctggcct
    16501 gccgatacta aagagggctg gtggcgctat cgtctgaaag ggtcaacctt gttaggtcac
    16561 gatcctgccg atccgctgaa acaacccgtt gaagcggaaa aaattaaaga tttctatcag
    16621 aaatggtaca ccccggatgc aatgacgcta ctggtggtgg gaaacgtgga tgcgcgctcg
    16681 gttgtcgacc aaatcaacaa aacgtttggc gaactgaaag gcaaacgtga aacgccagct
    16741 ccggtgccga cgctttctcc gctgcgtgcg gaagcggtga gtattatgac tgacgcggtg
    16801 cgtcaggacc ggttatctat catgtgggat acgccgtggc agccgattcg tgaatcagcc
    16861 gcactgctgc gctactggcg tgcggacctg gcccgcgagg cgctgttctg gcatgttcag
    16921 caagcgttaa gcgccagtga cagcaaagac atcggtcttg gatttgactg ccgtgtgctg
    16981 tatctgcgtg cgcagtgtgc catcaacatc gaatcaccaa acgacaagct gaacagcaac
    17041 cttaatctgg tggcgcgtga actggcgaag gttcgcgata aaggtctgcc ggaagaagag
    17101 ttcaatgcgt tagtggcgca aaagaaactg gagctgcaga aactgtttgc cgcctatgca
    17161 cgggctgata ccgatattct gatgggccag cggatgcgtt cgttgcaaaa tcaggtcgtc
    17221 gatatcgcgc cggagcagta tcagaaactg cggcaggatt tccttaacag cctgactgtg
    17281 gagatgttaa atcaggatct gcgtcagcag ttgtcgaatg atatggcgtt aatactgctg
    17341 cagccgaaag gcgagccgga atttaacatg aaagcgttgc aggcggcctg ggatcaaatc
    17401 atggccccat ctaccgccgc tgcgaccacc tctgtcgcca cggatgacgt acatcctgaa
    17461 gtgacggata ttccacctgc acagtaatgc ctgatggtga tgagttagca caaccatcag
    17521 gtatttatgg atgagctgga tagaggggta acactttatc cagccttttg catatgctgc
    17581 gtttacgctg gcatcgcctc acgcgggata atcgcgccgc gatactgaat aacggtactt
    17641 gcggtcaggt gcccgcgttt cgccgcgttt tccgcactgc cgcccgtcaa acgtaccgcc
    17701 agataaccgg cgctgaaaga gtcgccagct gcggtggtat cgatcacttt ttctttcggc
    17761 agtttcaccg ccggaacatc aactaacgct tcaccagcaa tggacaccag gcaagaatcc
    17821 gccccgcgtt tcaccaccac ttctttcacg cccgcgttat gggtgcgcgc aatgacgtct
    17881 tccaccggct gttgacccca cagcgcgtct tcgtcgtcca gcgtcaggaa ggcgatatcc
    17941 gtgcattcca gcatttgctg gtacacctgc tgtgtctctt ctttgctggc ccacaggcgc
    18001 ggacgatagt tattgtcgaa aatcactttt ccgcctttgg cgcggcattc gcgcagcagg
    18061 gaaagcagct tttcgcggct ggtcgggctt aagatcgcca ggctaatccc gctcaggtag
    18121 agataatcga aattcgccag ctcttcgcaa atcgccgcag actgctcgct cgccagccag
    18181 aatttggcgg cggcttcgtt acgccagtag tagaacgtac gttcgccggt gctgtcggtt
    18241 tcaatgtagt aaaggcccgg aagacggttt tccatccgtt gggtcaggga agtatcaacg
    18301 ttctcgccgt gccaggcgtc cagcatctgc tggctaaaac tgtccgttcc cagcgccgtt
    18361 acgtaatgaa cggttaatgc cgcaggatcg acctgacggg cgatatagac ggaagtgttc
    18421 agggtatcgc cgccgaaacc gcgcttaacg tccgcgcctt tctcggaaag ctcaatcatg
    18481 cattcgccaa tcacggcaat ctttttggac atagtcgtga acctgatctg taaaaaatta
    18541 gcgttagttt gcgctgtggt ggtttgctgg tcaaccatat taaaacagtg ttccattatt
    18601 tttttgagcc agaacgtatt cctgaaagat tccgttgtgg agtgaggaaa tttaccccgg
    18661 ccaggcggta aagttctgcc cttacgcgcc gataatcttt gtcgagtccg ggcagcatca
    18721 cttttaaaca caggacatct ttgatgataa ggcaggttat ccagcgaata agcaaccctg
    18781 aagcaagcat cgagagcttg caggaacggc gtttttggtt gcagtgtgag cgtgcttaca
    18841 cctggcagcc gatctatcaa acatgcgggc ggttaatggc cgtggagcta ttaacggtgg
    18901 tcacgcatcc cttgaaccct tcgcaacgcc tgccgccgga tcgctatttt actgaaatca
    18961 ccgtcagcca tcggatggag gttgtgaaag agcagattga tttgctggcg caaaaagccg
    19021 acttctttgt agagcacggc ctgctggcat cggtcaatat tgatggccct acgctcatcg
    19081 ccctgcgtca gcaaccaaaa atcctgcgcc agattgagcg tcttccctgg ctgcgtttcg
    19141 aactggtgga gcatatccgt ctgccgaaag attcaacctt tgcctcgatg tgtgaatttg
    19201 gcccgctgtg gctggatgat tttggtaccg ggatggcaaa tttctctgca ctaagtgaag
    19261 tgcgttatga ctacatcaaa atcgcgcgag aactgtttgt gatgctgcgt cagtcgccgg
    19321 aaggccgcac actcttttct cagcttttac atctaatgaa tcgctattgt cgcggggtga
    19381 ttgtcgaggg cgtagaaaca ccggaagagt ggcgtgatgt tcagaactcg cccgcattcg
    19441 ccgcacaagg ctggtttctt tcacgcccgg caccgatgga aacgctgaat acggcggttc
    19501 tggcgctata agctgcctca ttttccgccg ggctggacta tctttaggac tggcacagga
    19561 agaatatgag gaagcgaacg atgagcaagg caggcaaaat aaccgctgcg atttcagggg
    19621 ctttcttgtt gttgattgtc gtggcgatca ttttgattgc aacttttgac tggaatcgac
    19681 tcaaaccgac catcaaccag aaagtctctg cggagttgaa tcgtccgttc gctatccgtg
    19741 gcaatctggg cgtggtgtgg gagcggcaaa aacaagaaac tggctggcgc agctgggtgc
    19801 cgtggcctca tgtacatgca gaagacatca ttcttggtaa tccaccggat attcccgaag
    19861 tcacgatggt gcatttgccg cgagtagagg caacgctggc accgctggcg ctgctgacca
    19921 aaacggtctg gctgccgtgg atcaagctcg aaaagcccga cgcgcgcctg attcgcctct
    19981 ctgaaaagaa caataactgg acgtttaatc ttgccaacga tgacaacaaa gacgcgaatg
    20041 caaagccgtc ggcatggtcg tttcggctgg ataatattct tttcgatcaa gggcggatcg
    20101 ccattgatga caaagtaagc aaagcggatc tggaaatttt tgttgatccg ttaggcaagc
    20161 cgctgccatt cagcgaagtt actggatcga aaggtaaagc ggataaagaa aaggtgggcg
    20221 attacatttt tggcctgaag gcgcagggac gttataacgg tgaaccgctc acgggtacgg
    20281 gaaaaatagg cggtatgctg gcgctgcgtg gcgaagggac gccgtttccg gtacaggctg
    20341 atttccgttc aggtaatacc cgtgttgctt ttgatggcgt cgtgaatgac ccaatgaaga
    20401 tgggcggtgt cgatttacgg cttaaatttt ctggcgattc actgggtgat ctctatgaac
    20461 tgacgggcgt tctgctgccc gataccccgc cgtttgaaac ggatggtcgg ctggtagcga
    20521 aaatcgacac tgaaaaatcg tcggtctttg attatcgcgg ttttaatggg cgaattggcg
    20581 atagcgatat ccacggttct ctggtctaca ccaccggaaa gccacgacca aaactggaag
    20641 gtgatgtcga gtcgcggcaa ttgcggctgg cggacctggg accgttgatt ggcgttgatt
    20701 ccgggaaagg ggcagaaaag tcgaaacggt ctgaacagaa gaagggcgaa aaaagcgttc
    20761 agcctgcggg caaagtgctg ccttatgacc gcttcgaaac cgataaatgg gacgttatgg
    20821 atgccgatgt tcgcttcaaa gggcggcgca ttgagcatgg cagtagcctg ccgattagcg
    20881 atctttctac tcatatcatc ctcaaaaatg ctgacctgcg cctgcaaccg ctgaaatttg
    20941 gcatggcggg cggcagcatt gcggcgaata ttcatctgga aggcgataaa aagccgatgc
    21001 aggggcgggc agatattcag gctcgtcgac tgaaactgaa agaactgatg cccgatgtgg
    21061 aactgatgca gaagacgctg ggggaaatga acggtgacgc ggaactacgc ggtagcggta
    21121 actcggtggc ggcgctttta ggcaacagta acggcaacct gaaactgttg atgaatgacg
    21181 ggctggtgag ccgcaacctg atggagattg ttgggctgaa tgtcggcaac tacattgtcg
    21241 gtgcgatatt tggtgacgat gaggtgcggg tgaactgcgc ggcggcgaat ctgaatattg
    21301 ccaacggcgt ggcgcgcccg cagatttttg ctttcgatac tgagaacgcg ttgattaatg
    21361 ttaccggcac ggcaagtttt gcttcggaac agctggattt gactattgat ccggagagta
    21421 aagggattcg gattatcaca ctgcgttcgc cgctgtatgt gcgtgggacg tttaaaaatc
    21481 cgcaggctgg ggtgaaagcc ggaccgctga ttgcccgtgg tgctgttgct gcggcactgg
    21541 caacgctggt aacaccggcg gcggcgttac tggcactgat ctcaccttcc gaaggggagg
    21601 ctaatcagtg tcggacgata ttgtcgcaga tgaagaagtg atgataattg ccggatgtct
    21661 gacatccggc aattaaacag atttagattt ttggcaaagt gtgctttgtt catgccggat
    21721 gcggcgtgaa cgccttatcc ggcctacaaa tccttgtgaa ttcaatatat tgtatggaaa
    21781 ttcagtcctg ataagcgtag cgcatcaggc ttttcacact tattacaacg actgatgtcg
    21841 cgtctcatgg gtcagcagca gggcgattaa cgtcaagcca gccatcgccg ccagatataa
    21901 ccccaccgca cctaacccgt agttagcctg caaccaggct gcgatatacg gcgcaacgga
    21961 agccccgaga atcgacgata cgttgtaaga gaacgatgct ccggtgtaac gcacttctgt
    22021 cggaaacagc tctggtaaca gcgcacccat cggcccgaag gtcagcccca tcagacttaa
    22081 ccccagcagc aggaaggcaa aaaccagaat cgggttgcca gaaccgagca gcgggttaaa
    22141 ggcgaacagc gcgaacagga tgatcagcgt ggtgatgatc accatgcttt tacggcgacc
    22201 aaaggcatca gccagtaatc cggcgactgg caccatcacg ccaaaaccaa taactgccat
    22261 catcagcatc cacaacactt cgttacgcgg caggccaagc ccaactggcg cggcggcggt
    22321 actgaaggtc atagagtaga ccgtcatgat gtaaaacagc gtataggttg ccagcataat
    22381 gaacgtaccc agtacggtta cgcgaacatg tttggtcagc agcgtaccca gcgggatctt
    22441 cacctgtttt ttcgctttag cgactttctc aaacaccggc gactcatgca gcgacacgcg
    22501 aacatacagg ccgataatga ccagcaccgc cgagaagata aatggcacac gccagcccca
    22561 gctcataaac tgctcatcgg tcagtagcca ggaaagcagc aagaaagtgc cgttggcaaa
    22621 gaagaagcca atcggtgcgc ctagctgtgg gaaggagcca tacagtgcac gtttgcgcgg
    22681 tggggcgttt tcagtcgcca gcagcgccgc gccgccccat tcaccgccta agcccagacc
    22741 ctgaccaaat cgagccagcg ccagcagcag tggggcgaaa ataccaatcg tggcatagcc
    22801 cggcagcaga ccaatcacca cggtcgaaat ccccatcgtt agcaacgagg cgaccagcgt
    22861 cgctttacgc ccaacgcgat cgccaaaatg accaaaaacg gcagagccaa tggggcgcgc
    22921 gacgaaggcg atggcgaagg tggcgagcga ctgtagcgtt gctgccgttg gatcgccctg
    22981 cgggaagaag atatgcggaa acacaataac ggccgcagtg gcgtaaatgt aaaagtcgaa
    23041 gaactcaatg gccgtgccaa tgagagaggc gacaaggact ttattacgcg agttgatcgg
    23101 cgtgtattct tgttcgtggt cgagtgttgt ggctgttgct tgcataatct tttcttattt
    23161 tttggctaac gaatagccat attacgcaca gcaaaagtga tatttcaatg tgtaacaaat
    23221 cgcggaaatg gtcaaaaacc aggcaaaaag gggataatgt ttaggccagt gatttttctt
    23281 ctatgaaatg cttcacataa ttttataaat agtggataaa actggtttca ggttaaataa
    23341 aagttaccaa ctggatttat atgctggggt tatgagttgg agtgaaattg tttgctccca
    23401 aatagatggc tgaagaggct ctggggaaga gtttcaagag gttatgtgcg cattatagcc
    23461 ggatgcgatg ctggcgcatc ttatccggcc tacggggagc caggccgaat aaaatcgtat
    23521 gccggataag gcgtttacgc cgcatccggc ctattttaag gctgcgtttt ccctggcatt
    23581 cgcgggtcgt ctttatattc ggcggtggca atccacgccg cgcaaaacag cgtcaaacgg
    23641 gcgaagaagt agaaaaatgc catcagcccc agcacggaac caaatgctgc gccagagggg
    23701 gatttcatca gcgatggcag ggtgtaggtc atcacgattt taatcacttc aaaaccaata
    23761 gcagccagaa atgttccgcg aatcagggct tttttgcgcg gacggtggcg cggcaaccgc
    23821 cagaagatcc agaagaaaag cagatagttg gcgaagatgg aaatcgccag gccaatcaat
    23881 cgccacgtcg gtttcagcca ctcaatgctg ttcaggtgca gggcgctaat aatcatttgc
    23941 tgcgccgaac cggcaaccga ggtgatcgaa agcgtcacaa tcagcgcaat caacaaacca
    24001 atcagtgaaa taaaatcacg cagatatttt acccagaact tctcctgatc ttgcggcgag
    24061 cgttcccaga catcgcgcga ctgggcacga atcgcttcac gcaggttacc catccagttg
    24121 atgccggaat aaagtgccac cgccaggccg acaagcccta cagtcgtacg ctgctgaacg
    24181 gcggtgttga tggtgttttt caacgtggcg gctagtgtcg gatcgctgat gttttgcaga
    24241 attttgtcga agatatcctg tagcaacatc ggatgggagg ccagcacaaa gccccctgcg
    24301 gcaaacgaca ccatcaaaat cgggatcatc gacaaaaatg agaaataggt gatagccgca
    24361 ccaaactggt tacccagccg atcgttaaag cgttctgtcg cgcgaataag gtgcgcaata
    24421 accggttggc gttggacttt ttcggcagtg gtggtgacgg tttccagcgc ctggctaact
    24481 ttgccgcctt tctcgctatt atccaactgc ttaatcggct cgtgctccag atcctgggtg
    24541 ggacgtttga tctcgttttc ctgcgtcatc cgctcttttg tccttctgtc gtggtcattc
    24601 ttaaaaagta tagtcggtca gtccaggtgc tctttcatta cgccagccag ccactgcata
    24661 aacaggttta cacgccggga aagctcccga cgctgcgggt aaaccagcga aagggagagc
    24721 ggctcggcac ggtagcctgg caacacttca ataagccgcc cggcacgcag ggcttcgcgc
    24781 acggcaatgc gcgggatctg aataatcccc agaccggcaa gaccggcggc gagatagttt
    24841 tcgctactgt ttaccgtcaa catgccgcca gacttaaacc actggacgcc attaacgctg
    24901 gcaacctcaa aacctaacgg atgtacaccc aggtgcggtg tataacgcac tattgcgtgt
    24961 gaagtcagat catcggggct ttgcggataa ccaaagcgcg tcagatagtg cggactggca
    25021 cagttgacca tggttagttt gccgagggga cgggcgataa cgccatcttc cggtaacgcg
    25081 ccagtgcgta tcacgcaatc aaaaccatca tgaagaatat ctaccggacg gttatggcta
    25141 ctcagttcca gctcaattcc cggatgctga tagagaaatt ccgacagacg cggcagtaac
    25201 aggtttttcg cgattcctga cgggatgtcg atgcgtaatt taccgctgat actggtggca
    25261 tcctgttgaa acagaccgtc cagttcgctg agattactca acacatcttt tgcccgttga
    25321 taataggtca tgccttctgg cgtgagtttg acccgtcgcg tagtgcgctg gagaagctgg
    25381 gtgccaagtt gatgttccag tgcctgtatc tggcgcgaaa cacttccctt tggcaaagca
    25441 aagaaatccg ccgcgcggga aaaactttcc agctccgcga ctttgatgaa caactgcatt
    25501 gcgtgaattt tatccatttc tttgaccgat tgttgttcac agcgaaacag taacgcgcat
    25561 atcgcattgt ttattgtttt ttctaacatc taatgccggg tttcacaatg ttgtcatgac
    25621 gaatgtggcg atttctcagc aggaaaaggg cggaatagct ttatgagaaa ggtgaatccc
    25681 cttaaacaga gcgtattttt cagtgattcc ctttaaagtg aatagaggac gtattggcta
    25741 tttgtgctta tttttgtctc attgccattt gttattttgg cttgtagtcg cggaatttca
    25801 tttttaattg catgcgtact aaccaccaat ataaggaaat cctcaaaatt gttttaaata
    25861 gtgccctcga catgtaattt caggcgttta gctgtaacta tttatatcgg taactatttt
    25921 ctacgcgtca tctctgtaga atggccaccg ccaaatggcg aattcttatt ttttctcgcg
    25981 ggtaataccc ttccactact taagtctttt taacgtattc aggtggagat tcagttatgc
    26041 aaatagtcat gtttgacagg cagtcaatat ttattcatgg aataaaaatc agtttacagc
    26101 agcgtattcc aggagtgagt attcaggggg tcagtcaggc agacgagtta tggcaaaagc
    26161 tggaaagtta ccctgaagcc ttagttatgc tcgatggcga tcaggatggt gagttttgct
    26221 actggttgct gcaaaaaacc gtggtgcagt ttcctgaggt taaggtgtta atcacggcga
    26281 cggattgcaa taaacggtgg ttacaggaag ttatccattt taatgtgctg gccattgtgc
    26341 ctcgcgattc aaccgtcgag acatttgcgt tggcggtaaa tagtgcggcg atggggatga
    26401 tgtttctgcc tggagactgg cggacgacgc cggaaaagga cattaaagat ctcaaatcgt
    26461 taagcgcccg ccagcgggag attttaacca tgttagcggc gggcgaatca aataaggaga
    26521 tcggcagagc gctgaatatc agtaccggaa cggtaaaagc gcatctggaa tccctgtatc
    26581 gccgtctgga agtgaaaaat cgcacccagg cggcaatgat gttaaatatc tcctcctg
//
LOCUS       DAZSNO010000044        25706 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZSNO010000044 DAZSNO010000000
VERSION     DAZSNO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25706
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.044"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..905)
                     /locus_tag="ACHWN2_001772"
     CDS             complement(<1..905)
                     /locus_tag="ACHWN2_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFR8931337.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFLEGG
                     FALHGNSKIFGAVGISLAMYFTAAP"
     gene            1200..1874
                     /locus_tag="ACHWN2_001773"
     CDS             1200..1874
                     /locus_tag="ACHWN2_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR8931338.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(1913..3574)
                     /locus_tag="ACHWN2_001774"
     CDS             complement(1913..3574)
                     /locus_tag="ACHWN2_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR8931339.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(3770..4198)
                     /gene="ibpB"
                     /locus_tag="ACHWN2_001775"
     CDS             complement(3770..4198)
                     /gene="ibpB"
                     /locus_tag="ACHWN2_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR8931340.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(4310..4723)
                     /gene="ibpA"
                     /locus_tag="ACHWN2_001776"
     CDS             complement(4310..4723)
                     /gene="ibpA"
                     /locus_tag="ACHWN2_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR8931341.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(4717..4789)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            5028..5360
                     /locus_tag="ACHWN2_001777"
     CDS             5028..5360
                     /locus_tag="ACHWN2_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR8931342.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(5362..6600)
                     /locus_tag="ACHWN2_001778"
     CDS             complement(5362..6600)
                     /locus_tag="ACHWN2_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR8931343.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTWTPDSQWLVFDVRPSGAS
                     FTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFH
                     HRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLD
                     PALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGW
                     VGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVV
                     QRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKL
                     THNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSP
                     DGQWLAWMEGGQLWITETDR"
     gene            6701..7801
                     /gene="cbrA"
                     /locus_tag="ACHWN2_001779"
     CDS             6701..7801
                     /gene="cbrA"
                     /locus_tag="ACHWN2_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR8931344.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(7762..9099)
                     /locus_tag="ACHWN2_001780"
     CDS             complement(7762..9099)
                     /locus_tag="ACHWN2_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8931345.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALCFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(9174..10322)
                     /gene="dgoD"
                     /locus_tag="ACHWN2_001781"
                     /pseudo
     CDS             complement(9174..10322)
                     /gene="dgoD"
                     /locus_tag="ACHWN2_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(10319..10936)
                     /gene="dgoA"
                     /locus_tag="ACHWN2_001782"
     CDS             complement(10319..10936)
                     /gene="dgoA"
                     /locus_tag="ACHWN2_001782"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR8931346.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(10920..11798)
                     /gene="dgoK"
                     /locus_tag="ACHWN2_001783"
     CDS             complement(10920..11798)
                     /gene="dgoK"
                     /locus_tag="ACHWN2_001783"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR8931347.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(11795..12484)
                     /gene="dgoR"
                     /locus_tag="ACHWN2_001784"
     CDS             complement(11795..12484)
                     /gene="dgoR"
                     /locus_tag="ACHWN2_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR8931348.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            12762..13418
                     /gene="yidX"
                     /locus_tag="ACHWN2_001785"
     CDS             12762..13418
                     /gene="yidX"
                     /locus_tag="ACHWN2_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFR8931349.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(13464..14276)
                     /gene="yidA"
                     /locus_tag="ACHWN2_001786"
     CDS             complement(13464..14276)
                     /gene="yidA"
                     /locus_tag="ACHWN2_001786"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR8931350.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(14390..14788)
                     /locus_tag="ACHWN2_001787"
     CDS             complement(14390..14788)
                     /locus_tag="ACHWN2_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR8931351.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(15028..17442)
                     /gene="gyrB"
                     /locus_tag="ACHWN2_001788"
     CDS             complement(15028..17442)
                     /gene="gyrB"
                     /locus_tag="ACHWN2_001788"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR8931352.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(17471..18544)
                     /gene="recF"
                     /locus_tag="ACHWN2_001789"
     CDS             complement(17471..18544)
                     /gene="recF"
                     /locus_tag="ACHWN2_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR8931353.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(18544..19644)
                     /gene="dnaN"
                     /locus_tag="ACHWN2_001790"
     CDS             complement(18544..19644)
                     /gene="dnaN"
                     /locus_tag="ACHWN2_001790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR8931354.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(19649..21052)
                     /gene="dnaA"
                     /locus_tag="ACHWN2_001791"
     CDS             complement(19649..21052)
                     /gene="dnaA"
                     /locus_tag="ACHWN2_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR8931355.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            21349..21429
                     /locus_tag="ACHWN2_001792"
     CDS             21349..21429
                     /locus_tag="ACHWN2_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931356.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            21659..21799
                     /gene="rpmH"
                     /locus_tag="ACHWN2_001793"
     CDS             21659..21799
                     /gene="rpmH"
                     /locus_tag="ACHWN2_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR8931357.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            21816..22175
                     /gene="rnpA"
                     /locus_tag="ACHWN2_001794"
     CDS             21816..22175
                     /gene="rnpA"
                     /locus_tag="ACHWN2_001794"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR8931358.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            22139..22396
                     /gene="yidD"
                     /locus_tag="ACHWN2_001795"
     CDS             22139..22396
                     /gene="yidD"
                     /locus_tag="ACHWN2_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR8931359.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            22399..24045
                     /gene="yidC"
                     /locus_tag="ACHWN2_001796"
     CDS             22399..24045
                     /gene="yidC"
                     /locus_tag="ACHWN2_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR8931360.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            24151..25515
                     /gene="mnmE"
                     /locus_tag="ACHWN2_001797"
     CDS             24151..25515
                     /gene="mnmE"
                     /locus_tag="ACHWN2_001797"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR8931361.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6236 a         6932 c         6543 g         5995 t
ORIGIN      
        1 tccggtgcgg cagtgaagta catcgctaaa gaaatgccca cggcaccaaa gatttttgag
       61 ttaccgtgca gggcaaaacc gccttccagg aacaacgatt tcagtggctc ggcgctcaga
      121 ctgaactctt gcagatgctg cgcccagtac atctgaatgc cgccttcaac agctgccgga
      181 ccaaagataa acggtccgta gatgaagtgg tgtaaaccgg ttgggatcag aatacgttcg
      241 aggaaggtgt aaacccacac cccaagcgca cccgccgaac gcaggaatgc ttgcagagat
      301 tcaatcccca tttgtacttt tggccagccg agcaatgtca gccaggcaca ggggatcatc
      361 accaggaagg cgataatcac cacataagac gtcccctgga aaatgccgag aaaaaccggc
      421 agttttttat cgaacagacg gttatgcagc gccgtcacaa tgccggaaat gataattgcg
      481 ccgataatgc tggtatcgag ggttttaatc ccggccatca ttgtcagacc gctacctgcc
      541 accgcgtcct gagtgaaatc gacgccgaag tagcttcccc aggtcattcc catcgcattg
      601 atgaaatagt tccaggtcag gaaactcacc atcaccgcca gacaagcacg tccctgcgct
      661 tgcttagcaa ggccaatggg taaaccgaca gcaaaaatca gcggcatatt acggaatacc
      721 gtccaaccgc cctcttcaat aatgtgtacg atttgcgcga ataaactgtt cggatcggtc
      781 agtgattccc cgacaaacat cgggttttgc agcaagatgg caagacccac cacaatcccg
      841 gcgaagggaa acagcagcac tggcgtgaac atcgcgccgc caaagcgttg aatttgactg
      901 agcatttcca catcctcttt tctcaattct gtaagtagga agcctgattg gttatgcggc
      961 agacctggag cgaggatagg aagatgcgat cggcagaaca tgacgaccca atgggattcg
     1021 tgatcgcttt catgcttttc gttgggcggt agagatctac gttttcgcga aaaagtagac
     1081 atctgtgagc acaggtacac agcatcgaac acatctttaa aaaaaagatt tttttcaatc
     1141 gattaagcag aacttgtggg cgcattaccc gtgcttgcag gcaaaaaaga gagctagaga
     1201 tgatctacaa aagcattgcg gagcggttaa gaattcgact taattccgcg gatttcacgc
     1261 taaacagcct tcttcccggt gaaaaaaagc tggcggaaga gtttgcggta tcgcggatga
     1321 ctatccgtaa agccattgac ctgctggtag cgtgggggct ggtggtccgc cgccacggca
     1381 gcggcactta cctggtgcgc aaagatgtgc tgcatcaaac cgccagcctg accggactgg
     1441 tggaggtgtt aaaacggcag ggaaaaacgg tcaccagcca ggtgctgatt tttgaaatca
     1501 tgcctgcgcc tccggccatt gccagccagt tacggattca aatcaacgag cagatctact
     1561 tctcccgtcg cgttcgtttt gtggaaggga aaccgctaat gctggaagac agctatatgc
     1621 cggtaaaact gttccgtaac ctttcgctgc aacatctgga agggtcgaag tttgaatata
     1681 ttgaacaaga gtgcgggatt ttgattggcg gtaattatga aagcctgatg ccggtgctcg
     1741 ccgatagact gctggcgcgg caaatgaagg tagcggaaca cacgccactg ctgcggatca
     1801 cctcgttgtc atatagcgag agcggggagt ttttgaatta ttcagtgatg ttcagaaatg
     1861 ccagcgaata ctaggtggag taccatttac ggcgactcca cccggaaaaa agttaaccga
     1921 tactccagaa gagcaccgcc agtaattggg gggtgataat gcgcaggaac atcactaacg
     1981 gatagacagt ggcgtaagag agcgccgccg caccactggt tggatgaaga ttattagcaa
     2041 acgccagcgc cggaggatcg gtcatggaac ctgccagcat cccgcacatg gtcaggtaat
     2101 tcattttggc taacatccgc gccagaatgc caacagtaat cagcggaacg gcggtgatca
     2161 gggcaccata accaatccag cttagccctt cgccattgac cagggtattc acaaaatccc
     2221 caccagattt cagaccaacg accgagagga acagcacgat ccccagctcc cgcagcgcga
     2281 ggttggcact tggcggcata aaccagtaca gcttgccgat actgccgata cgcccgagga
     2341 tcaacgccat aatcagcgga ccgcccgcca gccccagttt caacgcggcc gggaatcctg
     2401 gcacaaagac gggaatagaa cctaacaata cgccaagccc gatgccaata aacactggca
     2461 gcatctgaac ctgttgcagt ttttgctgcg cattccccag cacattggca acggcatcaa
     2521 ttgcggacgg acgccccacc aggttgagga tatcgccgaa ctgcaggctg atatcgccac
     2581 tggcgaccag ttcgaccccg gcacggttca ggcgcgagat aacaacgtca tagcgttctt
     2641 taaagtgcag gtcgcgaata cgttttccga gcacgttttc attggtgacc accacacgct
     2701 cgacgcgcaa atcagtgcct ttcgtagaca gcgaggtatc gacctcctga ccaatcacca
     2761 gttgcgcatt atgtaaatcc gctggctgcc ccaccagatg cagcaaatcg cccagttgga
     2821 taatggtatc tggcgaaggt acttttaggg tttcttctcg tttcagacgc gagcagataa
     2881 ttttgtcgcc gttgagaatc ggtacatctt taatggctaa atcatgcagg ttagggttct
     2941 caacgcgaat attgatagtc ttgatcagcg cgccgccatt ggtgcgtgaa gactcgtgct
     3001 gctgagcttc tgtctcgaca ttgacgcgga aaataacccg caacatccac atggtgaaca
     3061 aaatcccgca aatgccgaat ggatacgcca tcgcgtaact catccccatc tgatcgacca
     3121 tttccattgg tgtacccagg tcgcgcaaga tctgctgccc tgcccccagc gctggcgtat
     3181 tggttaccgc accggagaaa atccccagca ctaccggcag tggaatatca aacagtttat
     3241 gcaggatggc ggtaaccaga ccaccgatga tgacgatcag aacagcaaac aggttgaggc
     3301 gtaatccgga gacgcgcaat gaggcaaaga agcccggccc tacctgaatc ccgatggtat
     3361 aaacgaacag gatcaggcca aattcctgaa taacatgcag catatcgcta cttaatgtca
     3421 tccccgcctg agaaacaaaa tggccgacga tgatcccacc aaacagcacg ccgccaatac
     3481 ctaatcctat gccgcgaaat ttgacgttgc cgataaacaa accgacgact gccaccaaag
     3541 ccagaatact gaccgttaat gctatatcac tcatcgtcca tttctcttga gataacattt
     3601 taattgctac ggattatggc tgaatcggtg aagcagattt gccttatagc gcacattatc
     3661 acgggaaaga acgtgccgaa atatcttaaa cagtcgaaat ttaacgcatt gttgaagaag
     3721 aaagacaaaa aaagccccac cagaatggcg gggcaaagag aatagctagt tagctattta
     3781 acgcgggacg ttcgctgata gcgatacgct gcgctgcgat gggttcaggc tcattacgaa
     3841 ttaaatcaat atgcagtaaa ccgtttacga aggttgcgcc agagacttcc atattttcag
     3901 ccagcgtaaa actcaggcta aatggctgat tcataagccc ttgatgcagc cattttttct
     3961 cttcttttgg ctgctccggc gtgcctttta cgctcaggcg cgtgccttcc agttgaatct
     4021 ctaaatcttc ctgacggaaa cctgccagcg caagggtaat gcggtagtgg ttatcgtcgc
     4081 ttttctcaat gttgtacggc gggaagctct ggctttcacc ggcgttttgc agtgcgttgg
     4141 ccagtttgtc aaaaccgatc cattgacgca tcagtgggga taaatcgaag ttacgcatag
     4201 tcatttctcc ttctaagaag cgagtaagta cctgcaaatc cgaagattcg catatgctcc
     4261 ctgacggcga gcatggagat gtcaggccgc gccaggcggc cttagggaat tagttgattt
     4321 cgatacggcg cggttttttc gcttccggaa tcacgcgttc gagatcgata tacagcaaac
     4381 catttaccag gttagcccca cgaacatgaa tgttctcagc taactggaat ttgcgttcaa
     4441 agttgcgttc agcgatgccc tggtacagat aggtgcgctc tttttgttcg tcggcgtgag
     4501 cacctttcac caccagcaga ttatcctggg cggtaatttc cagttcgctc tcagcaaaac
     4561 cagccacagc gatagcaatg cggtaatggt tttcgtctac cagttcaacg ttatacggag
     4621 ggtagccgcc attactctgg ctctggttgt tttctaagtg gttaaacaaa cggtcaaatc
     4681 caatagcaga acggtaaagc ggggataaat caaagttacg cataatcaat agctcctgaa
     4741 atcagcgaga atgtaagacc ttccacaatg gacaggtcag gtagccagaa cacccatcag
     4801 gcgcgtcctc atcgactacg atgtaaaatg ggtctggaaa tgaacttttc aagccttatc
     4861 agcggacttt tttttgcagt ttatggtcta ttgcatagac tgagggggca gcacgaaacg
     4921 ttaaagtgcg atagccgcca cagtgcgacg taatgatgat gttattttca gcaagggatc
     4981 gctataactc atcaggccaa cacccacgat caacagatga atttattatg ataagaaatg
     5041 tgttgttagc gttcatgata tgcagcggaa tgacattact cggaggatgc tccagcgtga
     5101 tgtcccatac cggggggaaa gaaggaacgt atcccggcac ccgcgccagc gccaccatga
     5161 ttggcgacga tgaaaccaac tggggcacta agtcgctggc gattctcgat atgccgttta
     5221 ccgctgtaat ggacacgctt ttactgccgt gggatgtgtt ccgcaaagac agctcggtgc
     5281 gctcgcgcgt tgaaaaaagc gaagccaacg cgcaagccac caatgcggtt attccaccgg
     5341 ccagaatgcc tgataactga tttagcgatc agtttcggtg atccacagct ggccaccttc
     5401 catccacgcc agccattgac catccggcga gaagaccacg gcgtccgcag aaggtggatt
     5461 ggcgtggttt tcggttaaat actcaacctc gccgctttgc gcatgggcac aaacaattcg
     5521 attatccagc acaaagccca accattctcc tgacggatgc cagttaaatg cagactgaat
     5581 atccgttttg ttatgggtta acttgcgcgg ttcgccgccc tgtggcgaga taagccacag
     5641 ttgcacaatg ccgttatcat cacgcattaa aaacgcgatt tgcgtaccct gcggattaca
     5701 gcgcacccag tggcgcggga cgttgactaa ccccggataa gcccgatgat gggtaaaggt
     5761 taaacgtcgc tgcacgacgc cacgcggtgg cgcgggtagg gttgtttccg ttccacttaa
     5821 cggcgcatca cctgccgatt tccagctagc ttcatcttgc ggtaactcaa cgataaatag
     5881 ctccggcact ttctcgccct ttggcgaaag tgtgtcgcca ataaacgcca gcgcgtgatt
     5941 tcctacccat ccttcttcat aagcacgatt gatttcatca ctgccaggct gcggcgtggg
     6001 cgtggtttta ctcaccagca cacaccagtg gctaccgctg tattcacgcg gatgttgctt
     6061 ttgtacgttg accgggccaa acggtgcagc aaccccgacg tttcgcaaat ccagcgccgg
     6121 atcgagttga tgcattacat ggtcgttata ggtaaagctc cccctttcac cgttcgggct
     6181 aaagacatgc acatggctgc caccgcgcag cgctcctggc gtatacggcg cggtaatatc
     6241 cattgcatcg agattgctca ccttgccgcc ttcagcaatg actccgcgtc gatgatggaa
     6301 gtcgtaatgc catgtttcat caggattttc agggccgtga ataaagacat atttctctga
     6361 cttcggatga acggtcacca cgccgacgtg tgcgccctgt gacgcgcgat agataacctc
     6421 gacctcgcct gtatggatat tcacacgctc aatggtctcg ccggtaaacg acgccccaga
     6481 aggacgcacg tcaaatacca gccactggct gtcgggcgtc caggtattgg tattggtatt
     6541 ggtattggta ttggtattgg tgagcaggtg attacgggga gcaaaggtta tctgtttcat
     6601 ggcgcggtcc tgatacaaaa cggttcccgc catcatactt cacaaggact tcactttcag
     6661 acaatagtct tttgccatcg tgatgaatgt gagtgaaaag atggaacatt tcgacgtggc
     6721 gattattggc ctcggcccgg cagggtcggc gttggcacga aagttagccg gcaaaatgca
     6781 ggtgatcgcg ctggataaaa agcaccagtg cggtactgaa ggtttcagca aaccttgcgg
     6841 cggtctgctg gcaccggacg cgcagcgatc ttttattcgc gatggactga cgcttcctgt
     6901 cgatgtgatc gccaatccac agattttcag cgtcaaaact gtcgacgtcg ccgcatcgct
     6961 cacgcgtaac taccagcgaa gctatatcaa tattaatcgc catgctttcg acttgtggat
     7021 gaaatcgctg atccccgcca gcgttgaggt ttatcacgat agcctgtgcc gaaaaatctg
     7081 gcgtgaggat gataaatggc atgtcatttt tcgtgcagac ggctgggagc aacatattac
     7141 cgcccgctat ctggtcggtg ccgatggcgc aaactcgatg gtgcggcgac atctctaccc
     7201 ggatcatcaa atccgtaaat atgtcgctat ccagcagtgg ttcgcagaga aacatccggt
     7261 gccgttctac tcctgcatct ttgataatgc gataactgac tgttactcat ggagtatcag
     7321 caaagatggt tattttatct ttggcggtgc ctatccaatg aaagacggtc agacgcgttt
     7381 cacgacgctg aaagagaaaa tgagcgcctt tcagttccag tttggcaaag cggtgaaaag
     7441 tgagaaatgc acggtgttgt ttccctcacg ctggcaggat tttgtctgtg gtaaggacaa
     7501 cgcctttctg attggtgaag cggcgggatt tatcagcgcc agctcgctgg aggggattag
     7561 ctatgcgctg gatagcgcag agattctgcg tgcggtgttc ctgaaacagc cggagaagag
     7621 caacgccgct tactggcgcg ccacacacaa actgcgttta aaactcttcg gcaagatagt
     7681 aaaaagccga tgcctgaccg caccggcttt aagaaagtgg attatgcgca gtggtgtggc
     7741 gcatattcca cagttgaaag attatccaac gcgcttcaca tcacccacca gcaggatgta
     7801 agagagcgcg ccaatcaacg cgacggcgga gatataaacc agtgcaggcg cgaaaccgta
     7861 accctgcgcc aggtacccca ccaccagcgg aacggtgatg ccgcccagac caccggcgaa
     7921 gttaaacacg ccgccggtta aaccaatcag gcgcatcggt gccagagaag agaccagcga
     7981 ccaggtaatc gaagcaaaac cgttgccgaa gaatgccagc gccatcaggc acataatcat
     8041 catcggatcg ttggtgtagt tagcgcccat aatgcaggtg gagatcagca agccgcagat
     8101 aatcggcgtt ttacgcgcaa agcccagtga aaagccctta cgtaccagca gatccgcgac
     8161 ccagccagag agcaggacgc cgacaaacgc cgcgaggaat ggcaccgtgg tcataaagcc
     8221 tgctttcagc gccgtgattc ctttttcctg ggttaaatag ttcgggaacc aggttaagaa
     8281 aaaacacagt gcagaagcca ccgcaaattg cccaagataa acgccgatca gtttacgatg
     8341 gaacaccagt ttccagtctt tggctgttaa cggctgacgc gcctctttct tcaccggcgc
     8401 atcaccatcc accagaccgc cgccatcacg aatgtaatcc agttcagctt tgctgatacc
     8461 tttggtcagg cgcggcggct gataaacctt aaaccaaatc agcgaccaga taatgccgat
     8521 accaccagtg acaatgaaca cccagtgcca gctcaacatc tcctgaatcc agatcagcag
     8581 cggagtcaaa aacgccagac cgacaaactg accagacgta taaaaaccaa cggcggaagc
     8641 gcgttcatgt tccgggaacc agctggtcac catccggtta ttggtcggga aagcgggcgc
     8701 ttcgaaaata ccggttatcg cacgcagacc aattaatgac attaatcccg tggcaaagcc
     8761 ttggaataaa gtcgccactg accaaccaaa tatcgcaata aaataagtca cgcgagaacc
     8821 tacgcgatct aaaaaccaac cgccggggat ctggcatagc gtataaagcc aggcgaaggc
     8881 cgaaaataca tagcccattt ccgctttcgt aatgccgaac tcttcctgaa tatgggcgga
     8941 agccacggcc aggttagcgc ggtcaacata acaaatgacc accgtaataa agatcatcac
     9001 cagcgtcaga taacgccgac gccccggctt tgctgcatta acgggaatat ccatagcgag
     9061 ctttctccag attttgggca tagcgaagcc gctcaccatg ccctgtaatt tacagagggt
     9121 tatatttttg tattgctgtt ttagtgcccg atgaggggct tacgtggcag gaattaccac
     9181 tctgctacgc tgttatcttc atgacgccag agcggattac gccagtccgg ggcattttta
     9241 ctgaactcaa taactttagc ttcgtcgatt tccacaccta agcccggttt cgttaacggt
     9301 ttaaagaagc cgccaaccat gctgaagtct tctttgtttt tcacaaagtc gagtaactcc
     9361 gcgcctttgt tgtaatgaat gcccatactt tgttactgaa gtaccgcgtt ataggaaaca
     9421 aagtcgatat gcaggcaagc cgccagtgca atcggtccga acggacagtg cggcgcaagg
     9481 gtcacatcat aggcttctgc cattccagca attttgtagc attcggtaat accgcctgca
     9541 tgggagagat ccggttgcag aatcgaaata ccgcctgcct ccagcacgcg tttaaaatcg
     9601 aaacgtgaga acatgcgctc acccgccgcc agtggaatat gcgtttgtgc cgccaatttc
     9661 gggtagtatt cggcttgttc cgccagcacc ggctcttcaa taaacagcgg gcgatacggc
     9721 tccagttctt taatcagcac tttcgccatt ggcgcgctga cgcgaccgtg gaaatcaaga
     9781 ccaaactcaa tctgattgcc aaaagcttca cgaatttgtg ccacggtgtt gactgccgca
     9841 tctaccgcgc gggagttatc aattagcccc agttcttcac aaccgttcag tttgaaggta
     9901 tcgaagccga tttcgcgcag ggttttaatg ccgtcgataa catccgccgg acgatcgccg
     9961 ccgacccaac tgtaggcttt aattttgtcg cgaaccaggc cgcccatcag ttgccagact
    10021 ggcgcattca gcactttgcc tttgatatcc cataacgcct ggtcgatccc ggcgatggcg
    10081 ctcatcagga ttggaccgcc acgataaaat ccggcgcgat acatcacttg ccataagtcg
    10141 ttgatgcgcg aaggatcctg accaatcaaa tagtcaccca gctcgtgaac tgccgcttcc
    10201 accgtacggg cgcggccttc aatcacgggc tcgccccaac cgaccacgcc ttcatcggtt
    10261 tcaattttca ggaacatcca gcggggaggt aaacgatacg tggtaatttt ggtgattttc
    10321 attgcactgc ctctcgatac gccttaacaa atgctgctgc ctgctgcgcg gtgcgttcta
    10381 cggactgccc ggcgcgataa agatcgctgc ctaagcccgc cccaacacaa cctgcgtcta
    10441 tccactgcgc caggttttct ggcgtcacgc cgccaacggc aaagactgcg atgtccggtg
    10501 gtaataccgc ttttaacgct ttgatgtatt gcggaccaaa agccgatgac ggaaatattt
    10561 tcagcgcctg cgcgcccgct tcgagcgcgg taaaggcttc ggtcgccgtc gcgcagccgg
    10621 ggcaaacggt catgccgtag cccaccgcgc ggcggatcac ttcactatgg atattgggcg
    10681 taacgatgag ctgacagccc atcctggcga gcgcatcgac ctgttcaggt ttcagtaccg
    10741 tacctgcgcc aatcaacgcc ttgtcgccat acgcatcaac gatggcggga atgctctgct
    10801 cccattgtgg ggaattcagc gggatttcaa ccgcgtcgaa cccggcgtca atcaccgcgc
    10861 caacatgcgc cagcgcctcg tcgggcgtaa tgccgcgcaa aatggcgatc agcgggagtt
    10921 tagtttgcca ctgcatgagc gatgctcctt ataccagcct gaaatgccgt gtcgcccgcc
    10981 accgccgcca cgtcgcaacc catcgcctga aaggcttgct ggtagcgcgc ggtcagcgat
    11041 gttccggcga caagggtgat ggcgtgttga tgggtcacat agtcgcgcat actggcaacc
    11101 tctgcgccaa tcaacaaacc agagagaaat tcgctgacct gttcgcgggg aagtgttccc
    11161 agcacatgcg aggcgcgaac ttcaaaaagc tgcggcaata tggcgggcgc attaaggccg
    11221 cgctcaaggc cagccgcgaa ggcatcggca gagttttcct gcggcggcaa acctgcgcca
    11281 atcaatgagt gatttaacag taaatgatgt aattcaccgg tcatcacggt gcgaaaatcg
    11341 ttgatttgct ggctatcggc ctgcacccat ttgcaatgcg taccgggcat aacataaaga
    11401 gaggaaggag ccagagctcg cgcgccgatc aattgtgttt cttcgccgcg catcacattg
    11461 tggttatcgt catgagagac gcataatccg ggaataatcc agatattgtc gccaacagac
    11521 gttaattgtt cgccaataga cgaaaaacgg gcaggaacag ataaatacgg agcaactttc
    11581 cagccgacat tgctgccgac cattcctgcc atcaccactg gcgtattttc ttcacgccag
    11641 tcggtcgtga cttctgctaa cactgcagcc ggagattttc cgttcaggcg cgtgacgcct
    11701 gcttctgatt gcctgctctc caggcagtgg tcgccctgat aaagccaggc gcgcagattg
    11761 gtcgatcccc agtcaattgc gatgtagcga gctgtcatgt gatttccttt aaccttcgtg
    11821 tcgagctggc aatcatggta agcgccgcct gctctgccgc atcgccgtcc tgatgccgta
    11881 tcgcatcgaa cagcgcctta tgttcctgga gcgtttgcgg catgttggcc tcatcgccca
    11941 tccaggttcg ttcaaaaacc gcccgctgca gcgaactgat cgcaatgcta agttgctgta
    12001 acaccgggtt atgcaccgac tgcagcaccg cctcgtggta gcgaatatcc gcttcgttaa
    12061 acgcttcgcg gtcctgattg ttggcaatca tctcgttcag cgccgattca atctgcgcca
    12121 gatcgctgga ggtcgcgcgc tctgctgccc aacgggcaat cgccggttcc accagatttc
    12181 gcacttcgct catggcactg ataagccgtg ggtcgtagtc gttttccagc acccattgca
    12241 gtacgtcagt gtcgaggtaa ttccactggt tacgcggtgc cacaaacgcc ccgcgataac
    12301 gtttcatttc aatcagccgc ttcgccatca gcgaacggaa cacctcacgg atgatgttgc
    12361 gcgaggttgc aaactcttca cagagctccg cctcagccgg aagtggcgag ccaggtacgt
    12421 atttgccgtg aacgatctgt ttacccagcg taatgacaat gcgatcggtt ttattgagag
    12481 tcatggagag tccttgtgct ctgtatgttc ttctctactt taccccgatc gatgcataac
    12541 gcggcaactt tgtagtacca gcgtgatgac gttcgcgttt gccgcgcgcg taatgtagta
    12601 caaacttata ttattgcgct acaatttaga tcaaaaaaag aacaacacat aaaaaaatga
    12661 catgcgtcgg gcagaaatct gaaaagggat atcaggcgct aaacaggagg gaaagaagag
    12721 tatgctttca acggcttagc tactcgttta aaggattaat catgaagttg aattttaagg
    12781 gattttttaa ggctgccggt ttattcccac tggcgctgat gctttcaggc tgtatctcgt
    12841 atgctctggt ttcccatacc gcaaagggta gttcaggaaa gtatcaatcg cagtcagaca
    12901 ccatcactgg gctatcgcag gcaaaagata gtaatggaac aaaaggctat gtttttgtag
    12961 gggaatcgct ggattacctt atcactgatg gtgccgatga catcgttaag atgctcaatg
    13021 atccagcact taaccggcac aatattcagg ttgccgatga cgcaagattt gttttaaatg
    13081 cggggaaaaa gaaatttacc ggcacaatat cgctttacta ctactggaat aacgaagaag
    13141 aaaaggcact ggcaacgcat tatggttttg cctgtggtgt tcaacactgt accaggtcac
    13201 tggaaaacct aaaaggcaca atccatgaga aaaataaaaa catggattac tcaaaggtga
    13261 tggcgttcta ccatccattt aaagtgcgat tttatgaata ctattcaccc agaggcattc
    13321 cggatggtgt ttccgcagca ttactgccag tgactgttac actggacatc attactgcac
    13381 cgctgcaatt tctggttgta tatgcagtaa accaataatc agtaagcggg caaacgcgtt
    13441 tatgctgttt gcccgcccac agattaattc agcacatact tctcaatagc aaacgccacg
    13501 ccatcttcaa ggttggattt tgtgacaaag ttcgccactt ctttcaccga aggaatagcg
    13561 ttatccatcg ccacaccgac gcctgcatat tcaatcattg cgatgtcgtt ttcctgatcg
    13621 ccaatcgcca tgatttcttc cggtttaata cctaacacgt cggccagtga tttcacaccc
    13681 gtacctttgt taacgcgttt atcgaggatt tcgaggaagt acggcgcact tttcagcacg
    13741 gtatatttct ctttcacttc ctgcggaata cgcgcgatag cctggtcgag gatggcgggt
    13801 tcatcaatca tcatcacttt caggaactgg gtattggggt ccattttctc cgcttcgcag
    13861 aacaccagcg gaattgtggc aacgaaggat tcatgcaccg tgtagtagct gatatcgcgg
    13921 ttagcggtgt acagcgtggt gcggtccagg gcgtggaaat gagaaccgac ttcgcgagag
    13981 agtttttcca ggaaacgata gtcgtcatag ctgagagcgg tttgcgccac ggtgctacca
    14041 tcagcggcct tctgtaccag cgcgccgtta taagtaatgc agtagtcgcc cggctgttcc
    14101 atatgcagct ctttcaggta gttgtgcaca cctgcatacg gacgacccgt cgttagcacg
    14161 acattcacgc cacgggcgcg agctgcggca atcgcatttt taacggcggg tgaaatggtg
    14221 tgatcgggca gcagaagggt gccatccata tcgatagcaa tgagtttgat agccatgagt
    14281 tcccaaggta gattagttcc tgacccatgc taacgcgatt ccgctcaaaa atcagtacaa
    14341 cacccgaggg aaaaggggat gcaacgcggg ttgcatgctc cccatttgct tagcggaaga
    14401 gtttcccttt cagcagttcc atgcctgcgg aaagcagatc gttattggct tgtgctgaca
    14461 cttcaccttg cggtgagagc gcatcaataa tcttcggcaa ttgctctgcc agtaaactgg
    14521 aagccgtact ggtatccacg ccaagttttt gcccgagatc ggacaccgca tttgtgccga
    14581 gcgccgactc cagttgctcg ccgctaactg attgatttct ctgttgatta ctcaaccagg
    14641 ttgagagaat ggcccctaag ccgccacttt gcagtttttc cagcagcacc tgaatgccac
    14701 cctgctcctc aacccaactt aaaatcgcct gatatttccc cgcatcgcct ttcagaaagg
    14761 caccgacaac ttcatcaaaa agccccatga taatcacctg taaagcgtta cgtgttgacc
    14821 caaaaagtat agatctgcgg gatgataatt gcggattgca gaaataaaaa gggcggagat
    14881 gatctccgcc cttttcttat agcttcttgc cggatgcggc gtgaacgcct tacccggcct
    14941 acaaaatcat gcaaaatcaa tatattgcag gagtctcgta ggactaataa gcgtagcgca
    15001 tcaggcacgc tcgcatggtt aacgccatta aatatcaata ttcgccgctt tcagggcgtt
    15061 ctcttcaata aacgcacgac gcggttctac ggcgtcgccc atcagcgtgg tgaacaactg
    15121 gtcagcggca atcgcatcct taacggtaac gcgcagcata cgacggcttt ccgggtccat
    15181 ggtggtttcc cacagctgtt ccgggttcat ctcgcccaga cctttataac gctggatgga
    15241 gaggccacga cgggactctt tcaccagcca gtccagcgcc tgctcgaagc tggctaccgg
    15301 ctgacgacgc tcgccacgtt cgataaacgc atcttcttcc agcaagccac gcagtttctc
    15361 acccagcgtg cagatacgac gatattcgcc accggtgata aactcgtgat ccagcggata
    15421 gtcagtatcc acaccgtggg tacgcacgcg aacaatcggc tcgaacaggt tttgttcggc
    15481 attggtgtgg acatcaaact tccactggct gccgtgctgt tctttgtcgt tcagttcgct
    15541 gaccagcgcg ttcacccagc gggtaacggt ctgctcatca gaaaggtcag cttccgtcaa
    15601 cgtcggctga tagataagct ctttcagcat tgctttcgga taacgacgct ccatgcggtt
    15661 gatcattttc tgcgtcgcgt tgtactcaga taccagtttc tctaacgctt cgccagccaa
    15721 tgccggtgca ctggcgttgg tgtgcagcgt tgcgccgtcc agcgcgatag agatctggta
    15781 ctgatccatc gcttcgtcgt ctttaatgta ctgttcctgc ttgcctttct tcactttgta
    15841 cagcggcggc tgagcgatgt agacgtgacc gcgttcaacg atttccggca tctgacgata
    15901 gaagaaggtc aacagcagcg tacgaatgtg cgagccgtcg acgtccgcat cggtcatgat
    15961 gatgatgctg tgataacgca gtttgtccgg gttgtactcg tcacgaccga taccacagcc
    16021 aagcgcggtg ataagcgtcg ccacttcctg agaagagagc atcttatcga agcgcgcttt
    16081 ctcgacgtta aggattttac ccttcagcgg cagaatcgcc tggttcttgc ggttacgccc
    16141 ctgcttcgca gagccgcccg cggagtcccc ttccaccagg tacagttcgg aaagcgccgg
    16201 atcgcgttcc tggcagtctg ccagtttgcc cggcaggcca gctaagtcga gcgcaccttt
    16261 acggcgggtc atttcacgcg cgcgacgcgc cgcttcacgg gcacgggcag catcgataat
    16321 tttgccaacc acgattttcg catcagttgg gttttccagc aggtattccg ccagcagttc
    16381 gttcatctgc tgttcaaccg ccgatttcac ctcagaagaa accagtttgt ctttggtctg
    16441 ggaggagaat ttcgggtccg gcacttttac ggaaacgacc gcaatcaggc cttcacgcgc
    16501 atcgtcaccg gtggcgctga ctttggcttt tttgctgtag ccttctttgt ccatgtaggc
    16561 attcagggta cgggtcatcg ccgcacggaa gcctgccagg tgagtaccgc cgtcacgctg
    16621 cggaatgttg ttggtaaagc agtagatgtt ttcctggaag ccatcgttcc actgcagcgc
    16681 cacttcaacg ccaataccgt ctttttcggt ggagaagtag aagatatttg ggtggatcgg
    16741 cgttttgttc ttgttcagat attcaacgaa cgctttgatg ccgccttcat agtggaagtg
    16801 gtcttctttg ccgtcacgct tgtcgcgcag acgaatggaa acgccggagt tgaggaacga
    16861 caactcacgc agacgtttcg ccagaatttc atattcgaac tcggtcacat tggtgaaggt
    16921 ttcgaggctg ggccagaaac gcaccatggt gccggttttt tcagtctcgc cggtaaccgc
    16981 cagcggagcc tgcggtacac cgtgttcgta gatctgacgg tgaattttac cctcgcgctg
    17041 aataaccagc tccagttttt gcgacagggc gtttactacc gaaacaccaa cgccgtgcag
    17101 accgccggac actttatagg agttatcgtc aaatttaccg cctgcgtgca gaacggtcat
    17161 gatcacttcc gccgccgata cgccctcttc cgggtgaata ccggtcggaa tgccgcgccc
    17221 gtcatcctgt acagagacag agttatcggc gtgaatggtg acgataattt ctttacagtg
    17281 acccgcgagc gcttcgtcga tagcgttatc taccacctcg aataccatgt ggtgcagacc
    17341 ggtgccgtca tccgtgtcgc cgatatacat acccgggcgc ttacgcaccg catccagccc
    17401 tttcaggact ttgatactgg aggagtcata agaattcgac atcaacgttt ctcgctcatt
    17461 tatacttggg ttaatccgtt attttaccct tttccacggt aaacatcttc gaattttcgt
    17521 ccgacatgtc tataacgtgt tcagcactga tcgcgctgac aaagacctgt gattgcgtcg
    17581 cttttaagcg gctggcaagc aacccgcgac gctcatcatc aagctcagag gcaaaatcat
    17641 ctatcaggta gagacaccgc cgcccgcttt cacgagtgag gaactctcct tgcgccagac
    17701 gtaaggcgca catcaacagc ttaagctgcc cacgcgataa ggtatcttcc accggcgcac
    17761 cgtcggcgcg aatgcgtaag tccgctttat gcgggccatg cgcggtgtag gttagctggc
    17821 gatcgcgttc aaaattacgt tccagcacct cagcgtactc tgtctctttc tcccagccgc
    17881 gctggaaaga gaaagtcaga gaaaactcag ggagaaattg cttacaggta tcatccatat
    17941 cggccgcgat accggcgcta tactccgcgc gccaggtgct gatttgctcc gccagcggga
    18001 tcagttcttt atcccacggg cgtagctgtt cgtaacgagt cacctggcgc agcgccgcat
    18061 tgcgctgctt gagcaatcgc ttgagattgc tccaggcggt gaaaaatcca ggttcgttgt
    18121 gaaagcatcc ccagtcgagg aatgctcttc tgtatttggg gccgccgttg agtaaagtaa
    18181 acccttctgg cgttatcaac tgcatcggca tcaggtgcgc cagttccgcg actttatgcc
    18241 cgtccgtacc gtcaatacgg actttgctgt cgccctgttt gtctttggtt aagccaatcg
    18301 ccgtctcgcg ctcttcgccc tgtaatcgcc cgtggagaac aaatgcctcc tgctcatggc
    18361 gaatgacgcg accaatctgc aaactgcgaa acgcccgacc atggccgagc gtatagatgg
    18421 cttccagcac gctggttttg ccactgccgt tggcacctac cagaaagtta aagccgggag
    18481 ataaagcgag atccgcggtt tcaatattgc ggaaatcgcg gatcaacaag cgggtgaggg
    18541 acattacagt ctcattggca tgacaacata ggcagccgac tgtgatgcgg catcttcaat
    18601 ctgcacgctg gaaaccgaat cggtcagcat catgcggacg ttttcgcatt tcagcgcgtt
    18661 cagaacatcc agcacatagc tgacgttgaa gccgatttcc atctccgcac cgctataggt
    18721 aacgtcgagg atctcttccg cttcttcctg ttccgggttg ttggcagtaa ttttcagctg
    18781 gttttcgctg acatacaggc gcacaccgcg gaatttctcg ttagagagaa tcgccgcgcg
    18841 agcaaacgcc tgcttgagca gatcgcagcc tgcttccaga tgtttgtccg ggttcttcgg
    18901 cagaacgcga cggtaatccg ggaagcgacc atccaccagt ttcgaggtga aaataaagtc
    18961 gccaacgtgg gcacgaatgt tattgctgcc aatctgcaca cgcagcagat tgtcgccgcc
    19021 gtcgagcata cgcatcagtt caatcacgcc tttacgcggt acgatcaccg aatggcttgg
    19081 caaagattga ccaattggca ttgaacagac cgccagacgg tggccgtcgg ttgccacggt
    19141 gcgcagttct tcaccttcgg tttcaaacag cataccgttt aagtaatagc gaacatcctg
    19201 atgcgccatc gaaaactggg ttgcttcaat cagacgcttc atcgtcgcct gcggcagggt
    19261 aaattcgact tcgctctgcc agtcatcgag gttcgggaaa tccgccgctg gcagggtaga
    19321 cagcgaaaaa cggctacgcc cggagcgcac cagcatccgt tcaccttcca gctgcacggc
    19381 aatttccgcg ccttcaggca gaccacggca gatatcaaag aatttgcgcg ccggaacggt
    19441 cgtcgctcct ggctcgtgtg gctgaaccag cgcaacgcgt gccaccattt ccatctcgag
    19501 atcagtaccg gtcagcgaca acgtaccgtc agcaacctgt aacagcagat taccgagaat
    19561 cggtagcgta ggacgaccac ctaacggacc gctcacctgt tgtagcggtt ttaataaatg
    19621 ctcacgttct acggtaaatt tcataggttt acgatgacaa tgttctgatt aaatttgaaa
    19681 aatcttcttt gatatcgtgg ctctcttcac gcaactgctc gatcttacgg caggcatgaa
    19741 gcaccgtcgt gtggtcacgc ccaccaaacg catcgccaat ctctggcaga ctgtggttag
    19801 tcagctcttt cgctagcgcc atcgccatct ggcgcggacg cgccaccgag cgggatcgac
    19861 gcttggaaag gagatccgcg actttgatct tgtagtactc cgccaccgtc ttctgaatat
    19921 tgtcgatggt gaccagtttt tcctgcaatg ccagcaagtc gcgcagcgcc tcacgcacga
    19981 agtcgatggt gatcgcccgt ccggtaaagt tggcgttagc aataacgcgg ttcagcgccc
    20041 cttccagctc acgtacgtta gatcgtagac gcttggcgat aaagaacgcc acttcacccg
    20101 gcaaacgaat gtcattttcg tcggcctttt tcatcaggat cgccacacgg gtttccagct
    20161 ctggcggttc gatcgccaca gtcagtcccc aaccgaagcg ggatttcaaa cgatcctcaa
    20221 cgccgttgat ctctttcgga tagcgatccg aggtgagaat gatctgttga ttaccttcca
    20281 gcagggcgtt gaaggtgtgg aaaaactctt cctgagatcg ttctttatta gcaaaaaact
    20341 gaatatcgtc gatcagcagt gcatctacgg aacggtagta gcgtttaaac tcttcgatcg
    20401 cgttgttttg cagggcttta accatgtcct gaacaaagcg ctcggagtgc atataaacca
    20461 ctttggcatt cggcttgcgc gccataatgc cgttacccac cgcatgcagc aggtgagttt
    20521 tacccagacc cgtgccgcca taaaggaaca acgggttata ggcaccgcca gggttatccg
    20581 ccacctggca agccgccgcg cgcgccagtt ggttagattt accttcaacg aagttatcaa
    20641 acgtgtgttt gacgtttacg ttagaacgat aggtcggttc tgccggggcc gggacgttat
    20701 cccaacctga gcgcgtagaa ggcgcagcac gttgcggctg cgtttgcgcc acctgtgcag
    20761 gggccgcgac gttgctcgtc actgccgctt gtggcgtttg cgtcaccggt ttggtgccga
    20821 cttcaaaacg cagctgtggg gcatccgctc cgcagaaact ggttagcagt ccattgatat
    20881 tattaaggta cttgtcccgt acccaatcga ggacaaaacg gtttggcgcg tacagggcca
    20941 gcgtgttatc gctcagttcc gcctgcaatg ggcgtatcca catactgaat tctgtggctg
    21001 gtaactcatc ctgcaatcgg gcaagacact gctgccaaag cgaaagtgac acggcggact
    21061 ccactcgaac aaaagtcgat aatgactaag gctgaaacat tcatgattgt tgacgtacgt
    21121 cgaaaagacc ctgcttgagg gtgacgcacg aaccgctgtc tgcggttata tgcccgatca
    21181 agatcctgca aaacgatcgg gaccgcggat catagcctaa actgcgcaag agatcttctg
    21241 tttctcacag attttcccta tttatccaca ggactttcca gaactcgcta agtgtaaacg
    21301 atcctgccgc gaggcgggca cgatttacgc cgcatattgg aaaatttaat gaccatagac
    21361 aaaaattggc ttaatcgatc taataaagat ccaggacgat ccttgcgctt tacccatcag
    21421 cccgtataat cctccacccg gcgcgccatg ctggtttcca ctggtgtgag gtcgtacatt
    21481 ttccctgcga aaaggtgcgg aaaagcgcgg taaataagga aagagaattg actccggtgt
    21541 gtacaattat tacaatccgg cctctttaat cacccatggc ttcggtgtcc atcgtttcat
    21601 ttttcggcgg atatccaata aagccattga atttattcaa gtttaggtag aaatcgccat
    21661 gaaacgcact tttcaaccgt ctgtactgaa gcgcaaccgt tctcacggct tccgtgctcg
    21721 tatggctact aaaaatggtc gtcaggttct ggcacgtcgt cgtgctaaag gccgcgctcg
    21781 tctgaccgtt tctaagtaat aaagctaacc cctgagtggt taagctcgca tttcccaggg
    21841 agttacgctt gttaactccc agtcaattca cattcgtctt ccagcagcca caacgggctg
    21901 gcacgccgca aattaccatt ctcggccgcc tgaattcgct ggggcatccc cgtatcggtc
    21961 ttacagtcgc caagaaaaac gttcgacgcg cccatgaacg caatcggatt aaacgcctga
    22021 cgcgtgaaag cttccgtctg cgccaacatg aactcccggc tatggatttc gtggtggtgg
    22081 cgaaaaaagg ggttgccgac ctcgataacc gtgctctctc ggaagcgttg gaaaaattat
    22141 ggcgccgcca ctgtcgcctg gctcgcgggt cctgatagcc ctcattcggg tctatcaacg
    22201 cctgattagt ccgctactcg ggccgcattg tcgtttcact ccaacctgtt caagctacgg
    22261 aattgaggca ttgcgcaggt ttggagtgat aaaaggcagt tggttgacgg tgaaacgcgt
    22321 attaaaatgc caccctttac accctggtgg tgacgatccc gtcccgcccg gaccatttga
    22381 taccagagaa cactaacgat ggattcgcaa cgcaatcttt tagtcatcgc tttgctgttc
    22441 gtgtctttca tgatctggca agcctgggag caggataaaa acccgcaacc tcaggcccaa
    22501 cagaccacgc agacaacgac caccgcagcg ggtagcgccg ccgaccaggg cgtaccggcc
    22561 agtggccagg ggaaactgat ctcggttaag accgacgtgc ttgatctgac catcaacacc
    22621 cgtggtggtg atgttgagca agctctgctg cctgcttacc cgaaagagct gaactctacc
    22681 cagccgttcc agctgttgga aacttcaccg cagtttattt atcaggcaca gagcggtctg
    22741 accggtcgtg atggcccgga taacccggct aacggcccgc gtccgctgta taacgttgaa
    22801 aaagacgctt atgtgctggc tgaaggtcaa aacgaactgc aggtgccgat gacgtatacc
    22861 gacgcggcag gcaacacgtt taccaaaacg tttgtcctga aacgtggtga ttacgctgtc
    22921 aacgtcaact acaacgtgca gaacgctggc gagaaaccgc tggaaatctc ctcgtttggt
    22981 cagttgaagc aatccatcac tctgccaccg catctcgata ccggaagcag caacttcgca
    23041 ctgcacacct tccgtggcgc ggcgtactcc acgcctgacg agaagtatga gaaatacaag
    23101 ttcgatacca ttgccgataa cgaaaacctg aacatctctt cgaaaggtgg ttgggtggcg
    23161 atgctgcaac agtatttcgc gacggcgtgg atcccgcata acgacggtac caacaacttc
    23221 tataccgcta atctgggtaa cggcatcgtc gctatcggct ataaatctca gccggtactg
    23281 gttcagcctg gtcagactgg cgcgatgaac agcaccctgt gggttggccc ggaaatccag
    23341 gacaaaatgg cagctgttgc tccgcacctg gatctgaccg ttgattacgg ttggttgtgg
    23401 ttcatctctc agccgctgtt caaactgctg aaatggatcc atagctttgt gggtaactgg
    23461 ggcttctcca ttatcatcat cacctttatc gttcgtggca tcatgtaccc gctgaccaaa
    23521 gcgcagtaca cctccatggc gaagatgcgt atgctgcagc cgaagattca ggcaatgcgt
    23581 gagcgtctgg gcgatgacaa acagcgtatc agtcaggaaa tgatggcgct gtacaaagct
    23641 gagaaggtta acccgctggg cggctgcttc ccgctgctga tccagatgcc aatcttcctg
    23701 gcgttgtact acatgctgat gggttccgtt gaactgcgtc aggcaccgtt tgcactgtgg
    23761 atccacgacc tgtcggcaca ggacccgtac tacatcctgc cgatcctgat gggcgtaacg
    23821 atgttcttca ttcagaagat gtcgccgacc actgtgaccg acccgatgca gcagaagatc
    23881 atgaccttta tgccggtcat cttcaccgtg ttcttcctgt ggttcccgtc aggtctggta
    23941 ctgtactata tcgtcagcaa cctggtaacc attattcagc agcagctgat ttaccgtggt
    24001 ctggaaaaac gtggcctgca tagccgcgag aagaaaaaat cctgattcgg tgagttttcg
    24061 ctaaaataag ggcggtcagt tgaccgcctt ttttcttttc gtagggcgga taagcaccgc
    24121 gcatccgcca cacaaagcaa caggaacatc atgagcgata atgacactat cgtagcccag
    24181 gccacgcctc cgggacgtgg cggcgttggc atcctgcgca tctccggctt caaagcccgt
    24241 gaagttgccg aaaccgtgct gggtaaactg cctaagccgc gctacgccga ttatcttccg
    24301 tttaaagacg ccgacggcag cgtgctcgat caggggattg cgctatggtt ccctggcccg
    24361 aactcgttca ccggcgaaga tgtgctggaa ctgcaaggtc atggcggtcc ggtgatcctc
    24421 gacctgctgt taaaacgcat tctgaccatt cccggcctgc ggattgctcg ccctggtgag
    24481 ttttccgaac gcgcgtttct taacgataaa cttgacttag cccaggccga ggcgattgcc
    24541 gatcttatcg acgccagttc ggaacaggcg gcccgttcgg cacttaactc gctgcaaggc
    24601 gcattctccg cacgggttaa tcatctggta gaagccctca cccacttgcg catttacgtc
    24661 gaagcggcaa ttgatttccc tgatgaagag atcgatttcc tctccgacgg caaaattgaa
    24721 gcccagctta atgacgtgat ggccgatctc gatgcagtgc gtgctgaagc acgtcagggt
    24781 agtttgttgc gcgaagggat gaaagtagtg attgccggac gtcctaatgc cggtaaatcg
    24841 agcctgttaa acgcgctggc gggccgtgaa gcggcaatcg taaccgatat cgccggaacc
    24901 acgcgtgacg tgctgcgtga gcatatccac attgacggaa tgccgctgca tatcatcgac
    24961 actgccgggc tacgtgaggc cagtgacgaa gtggaacgta ttggtatcga gcgcgcgtgg
    25021 caggaaattg aacaggccga ccgcgtgctg tttatggtcg atggcaccac gacagacgcc
    25081 gttgatccgg cagagatctg gccggaattt atcgcccgtc tgcccgcgaa actgccgatc
    25141 accgtggtac gcaataaagc cgatatcacc ggcgaaacgc tgggaatgag tgaagtgaac
    25201 ggtcacgcgt taattcgtct ctcggcaagg actggtgaag gcgtggacgt gctgcgtaac
    25261 catctcaaac agagcatggg ctttgacacc aatatggaag gcggcttcct ggcgcgccgt
    25321 cgccacttac aggcgctgga acaggcagcg gagcatttgc agcaaggtaa agcgcaactg
    25381 ttgggagcct gggcaggtga actgctggcg gaagagttgc gcctggcaca gcagaactta
    25441 agcgaaatca ccggggaatt tacttcagac gacctgctgg ggcggatttt ctccagcttc
    25501 tgtattggta agtaaccgcg cttacgaagc cgtattctga ctgtcagatg cggcttcgct
    25561 tcattgttag cactcctgtt atgcctcaac ccttttttaa acattaaaat tcttacgtaa
    25621 tttataatct ttaaaaaaag catttaatac tgctccccga acgattgtga ttcgattcac
    25681 atttaaacaa tttcagaata gacaaa
//
LOCUS       DAZSNO010000045        25663 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZSNO010000045 DAZSNO010000000
VERSION     DAZSNO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25663
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.045"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHWN2_001798"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHWN2_001798"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR8931362.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="ACHWN2_001799"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="ACHWN2_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR8931363.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="ACHWN2_001800"
     CDS             2630..3391
                     /locus_tag="ACHWN2_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR8931364.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="ACHWN2_001801"
     CDS             complement(3442..4326)
                     /locus_tag="ACHWN2_001801"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR8931365.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHWN2_001802"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHWN2_001802"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR8931366.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="ACHWN2_001803"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="ACHWN2_001803"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR8931367.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="ACHWN2_001804"
     CDS             6202..6474
                     /locus_tag="ACHWN2_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR8931368.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="ACHWN2_001805"
     CDS             6844..7920
                     /locus_tag="ACHWN2_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR8931369.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="ACHWN2_001806"
     CDS             7947..9326
                     /locus_tag="ACHWN2_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8931370.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="ACHWN2_001807"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="ACHWN2_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="ACHWN2_001808"
     CDS             10409..11245
                     /locus_tag="ACHWN2_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFR8931371.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="ACHWN2_001809"
     CDS             11250..12197
                     /locus_tag="ACHWN2_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR8931372.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHWN2_001810"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="ACHWN2_001810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFR8931373.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="ACHWN2_001811"
     CDS             13324..14082
                     /locus_tag="ACHWN2_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR8931374.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="ACHWN2_001812"
     CDS             14200..15558
                     /locus_tag="ACHWN2_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8931375.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHWN2_001813"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACHWN2_001813"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR8931376.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHWN2_001814"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACHWN2_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR8931377.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHWN2_001815"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACHWN2_001815"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR8931378.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="ACHWN2_001816"
     CDS             19503..20054
                     /locus_tag="ACHWN2_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR8931379.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="ACHWN2_001817"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="ACHWN2_001817"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR8931380.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="ACHWN2_001818"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="ACHWN2_001818"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR8931381.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="ACHWN2_001819"
     CDS             23307..24347
                     /locus_tag="ACHWN2_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR8931382.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..>25663)
                     /gene="gdhA"
                     /locus_tag="ACHWN2_001820"
     CDS             complement(24464..>25663)
                     /gene="gdhA"
                     /locus_tag="ACHWN2_001820"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR8931383.1"
                     /translation="SLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYK
                     GGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTL
                     MTELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRP
                     EATGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSG
                     TVVDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQ
                     NELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGL
                     EMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADA
                     MLAQGVI"
BASE COUNT         6519 a         5971 c         6625 g         6548 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctagctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatttgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagtaa tga
//
LOCUS       DAZSNO010000046        24654 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZSNO010000046 DAZSNO010000000
VERSION     DAZSNO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24654
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.046"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..386)
                     /locus_tag="ACHWN2_001821"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="ACHWN2_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="ACHWN2_001822"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="ACHWN2_001822"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR8931384.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="ACHWN2_001823"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="ACHWN2_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR8931385.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHWN2_001824"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHWN2_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR8931386.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="ACHWN2_001825"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="ACHWN2_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR8931387.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="ACHWN2_001826"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="ACHWN2_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR8931388.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="ACHWN2_001827"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="ACHWN2_001827"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR8931389.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="ACHWN2_001828"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="ACHWN2_001828"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR8931390.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHWN2_001829"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHWN2_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR8931391.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="ACHWN2_001830"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="ACHWN2_001830"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR8931392.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="ACHWN2_001831"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="ACHWN2_001831"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR8931393.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="ACHWN2_001832"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="ACHWN2_001832"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR8931394.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="ACHWN2_001833"
     CDS             10748..11212
                     /locus_tag="ACHWN2_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR8931395.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHWN2_001834"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHWN2_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR8931396.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFVWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /gene="selO"
                     /locus_tag="ACHWN2_001835"
     CDS             12236..13672
                     /gene="selO"
                     /locus_tag="ACHWN2_001835"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR8931397.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHWN2_001836"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHWN2_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR8931398.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHWN2_001837"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHWN2_001837"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR8931399.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHWN2_001838"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHWN2_001838"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR8931400.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="ACHWN2_001839"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="ACHWN2_001839"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR8931401.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACHWN2_001840"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACHWN2_001840"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFR8931402.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="ACHWN2_001841"
     CDS             complement(20505..20798)
                     /locus_tag="ACHWN2_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR8931403.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="ACHWN2_001842"
     CDS             complement(20795..22084)
                     /locus_tag="ACHWN2_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR8931404.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="ACHWN2_001843"
     CDS             complement(22140..23078)
                     /locus_tag="ACHWN2_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR8931405.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="ACHWN2_001844"
     CDS             complement(23098..23862)
                     /locus_tag="ACHWN2_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR8931406.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24182..>24654
                     /locus_tag="ACHWN2_001845"
     CDS             24182..>24654
                     /locus_tag="ACHWN2_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFR8931407.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         6103 a         5949 c         6485 g         6117 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgtctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg ggggcgacgg agcaagctgt tttccttgcc aacaatcttg caaaaaaaat
    24001 gcgattgcta aaacaaaagt gcaatttgcg gcaatatagt tattccatga ccgtacaatc
    24061 ttgcattcag tgtaaataat cttgtttagc tctcgctaaa cgcggttgta taaccgcaat
    24121 acacgccgta ttttatccgg catatattgt ttttgcttgc tcatggaata gggaggttgt
    24181 catgtatcaa cgctgttttg ataatgccag tgaaacgctg tttgtcgccg gtaaaacgcc
    24241 acggctttca cgttttgcat ttagcgatga tccaaaatgg gagtctggac atcacgttca
    24301 tgacaatgaa accgagctga tttacgtcaa gaaaggggtt gcaaggttta ccatcgattc
    24361 ttcgttatat gtcgcacatg cggatgacat tgtggtgata gaacgcggca ggctgcatgc
    24421 ggtggcctct gacgttaacg atccggcaac gacgtgtacc tgtgcgctgt acggctttca
    24481 gtttcagggg gctgaggaaa atcagttact gcaaccgcat tcttgtccgg taattgccgc
    24541 ggggcaggga aaagaagtca taaaaacctt atttaatgag ctaagtgtga ttttgccgca
    24601 aagtaaaaat agccaaacat cttcgttatg ggatgcatta gcctatacat tagc
//
LOCUS       DAZSNO010000047        24580 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZSNO010000047 DAZSNO010000000
VERSION     DAZSNO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24580
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.047"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(2..94)
                     /locus_tag="ACHWN2_001846"
     tRNA            complement(2..94)
                     /locus_tag="ACHWN2_001846"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHWN2_001847"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHWN2_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR8931408.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHWN2_001848"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHWN2_001848"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR8931409.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHWN2_001849"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHWN2_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR8931410.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="ACHWN2_001850"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="ACHWN2_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR8931411.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="ACHWN2_001851"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="ACHWN2_001851"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR8931412.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="ACHWN2_001852"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="ACHWN2_001852"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR8931413.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="ACHWN2_001853"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="ACHWN2_001853"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="ACHWN2_001854"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="ACHWN2_001854"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR8931414.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="ACHWN2_001855"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="ACHWN2_001855"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR8931415.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="ACHWN2_001856"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="ACHWN2_001856"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFR8931416.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="ACHWN2_001857"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="ACHWN2_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR8931417.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="ACHWN2_001858"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="ACHWN2_001858"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR8931418.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="ACHWN2_001859"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="ACHWN2_001859"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR8931419.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="ACHWN2_001860"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="ACHWN2_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR8931420.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="ACHWN2_001861"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="ACHWN2_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR8931421.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="ACHWN2_001862"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="ACHWN2_001862"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR8931422.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="ACHWN2_001863"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="ACHWN2_001863"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR8931423.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="ACHWN2_001864"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="ACHWN2_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR8931424.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="ACHWN2_001865"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="ACHWN2_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR8931425.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20948..21019)
                     /locus_tag="ACHWN2_001866"
                     /pseudo
     CDS             complement(20948..21019)
                     /locus_tag="ACHWN2_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="ACHWN2_001867"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="ACHWN2_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR8931426.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="ACHWN2_001868"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="ACHWN2_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR8931427.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..>24580)
                     /locus_tag="ACHWN2_001869"
     CDS             complement(23682..>24580)
                     /locus_tag="ACHWN2_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFR8931428.1"
                     /translation="ALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRD
                     IEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLL
                     GPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCV
                     IGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPS
                     RSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSAL
                     LFRRMALVELAVLVVIIVSRGA"
BASE COUNT         5797 a         6915 c         6306 g         5562 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga
//
LOCUS       DAZSNO010000048        24112 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZSNO010000048 DAZSNO010000000
VERSION     DAZSNO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24112
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.048"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(25..597)
                     /locus_tag="ACHWN2_001870"
     CDS             complement(25..597)
                     /locus_tag="ACHWN2_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR8931429.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="ACHWN2_001871"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="ACHWN2_001871"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR8931430.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="ACHWN2_001872"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="ACHWN2_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR8931431.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="ACHWN2_001873"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="ACHWN2_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR8931432.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="ACHWN2_001874"
     CDS             complement(3559..5340)
                     /locus_tag="ACHWN2_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR8931433.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="ACHWN2_001875"
     CDS             complement(5333..7105)
                     /locus_tag="ACHWN2_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR8931434.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="ACHWN2_001876"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="ACHWN2_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR8931435.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="ACHWN2_001877"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="ACHWN2_001877"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR8931436.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10356
                     /locus_tag="ACHWN2_001878"
                     /pseudo
     CDS             8665..10356
                     /locus_tag="ACHWN2_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10421..11116
                     /gene="queC"
                     /locus_tag="ACHWN2_001879"
     CDS             10421..11116
                     /gene="queC"
                     /locus_tag="ACHWN2_001879"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR8931437.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="ACHWN2_001880"
     CDS             complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="ACHWN2_001880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR8931438.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11660..12031)
                     /locus_tag="ACHWN2_001881"
     CDS             complement(11660..12031)
                     /locus_tag="ACHWN2_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR8931439.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="ACHWN2_001882"
     CDS             complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="ACHWN2_001882"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR8931440.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14245..14535)
                     /gene="hupB"
                     /locus_tag="ACHWN2_001883"
     CDS             complement(14245..14535)
                     /gene="hupB"
                     /locus_tag="ACHWN2_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR8931441.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14726..17080)
                     /gene="lon"
                     /locus_tag="ACHWN2_001884"
     CDS             complement(14726..17080)
                     /gene="lon"
                     /locus_tag="ACHWN2_001884"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR8931442.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="ACHWN2_001885"
     CDS             complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="ACHWN2_001885"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR8931443.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="ACHWN2_001886"
     CDS             complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="ACHWN2_001886"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR8931444.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19538..20836)
                     /gene="tig"
                     /locus_tag="ACHWN2_001887"
     CDS             complement(19538..20836)
                     /gene="tig"
                     /locus_tag="ACHWN2_001887"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR8931445.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="ACHWN2_001888"
     CDS             complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="ACHWN2_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR8931446.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21802..22380
                     /locus_tag="ACHWN2_001889"
     CDS             21802..22380
                     /locus_tag="ACHWN2_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8931447.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22424..23899
                     /gene="ampG"
                     /locus_tag="ACHWN2_001890"
     CDS             22424..23899
                     /gene="ampG"
                     /locus_tag="ACHWN2_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR8931448.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5649 a         6647 c         5820 g         5996 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacag taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatggcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa ctggccgaac gctgtttttg
     8761 cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaggcgggat ggctggagtg
     8821 gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca cgccggaatc
     8881 gctacgcaat gcgatgatgg aacaggcact ggaaaccgga aagcagcaag atgtgctgga
     8941 gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccattta tgggcggaca
     9001 atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg aaccgctaca
     9061 accaggcttt ttgcccggcc gtgccgagca gcatctcgcc gggcagatat tttccggcct
     9121 gacccgcttc gataataata ctcagcgccc gattggcgat ttagcgcatc actgggaaac
     9181 ctctactgac aggttacgct gggactttta tcttcgttca accctacact ggcataacgg
     9241 cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac aactgccagc
     9301 actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc agtgtctgac
     9361 cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct attgcagcca
     9421 tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa cacaattcac
     9481 agcggagctg gtgcgcctgg agagccatga ttattaccat ttgcgtcatc cgctgcttaa
     9541 agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa ccagttgtcg
     9601 gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca gccaagtcag
     9661 tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc gactctccct
     9721 ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac aaacgttaga
     9781 agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga ctattccgca
     9841 ctggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt atcacctacc
     9901 gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg aaggctgtga
     9961 actcacaatt atttttcata acgcaaaaac tgggacgaca cgaccctact ggcacacgca
    10021 gacctcatga tgggcgacag attaattggc gaagcaccgg aatatactct ggagcaatgg
    10081 ctacgttgcg atccactatg gccacatgtt ttcgacgctc cagcatatgc acatctgcaa
    10141 tcgacgctgg acgcggtgca agtaatgcct gatgaggaaa accgatttaa tgccctgaaa
    10201 gcggttttta gccagttaat ggcagacgcg acgctgacgc cgctgttcaa ctatcactat
    10261 cgcattagtg cccctcccgg cgtgaacggt gtgcgactga caccgcgcgg ctggtttgaa
    10321 tttaccgaag cctggcttcc cgcgccgtcg caatgaatag ctggtccggg ttaatcgcag
    10381 gcgttaccat aacgctttta ttgttcaagc aggattatct atgaaacgtg ctgtcgttgt
    10441 gttcagtgga ggccaggatt ccaccacctg tctggtgcag gcattacaac aatatgatga
    10501 agtccattgc gtgacgttcg attacggtca gcgacatcgc gcagaaatcg acgtggcacg
    10561 cgaactggcg ctgaaactgg gagcacgcgc gcataaggtt cttgatgtaa cgctgctcaa
    10621 cgagctggcg gtcagcagcc tgacacgtga cagcattccg gtgcctgatt atgaacctga
    10681 agccgatggc atcccgaata cgtttgtccc agggcgtaat attttgttcc tgacgctggc
    10741 ggcaatatat gcgtatcagg taaaagcaga agccgtaatt accggcgtct gtgaaacgga
    10801 tttctccggc tacccggatt gccgcgatga gtttgtgaaa gcactaaacc atgccgtcag
    10861 tttgggcatg gcgaaagata ttcgttttga aacgccgctg atgtggattg ataaagcgga
    10921 aacctgggcg ctggcagatt attacggcaa actggattta gtccgtaacg aaacgttgac
    10981 ctgctataac ggcattaaag gcgacggttg cggtcattgt gcggcatgta atttacgcgc
    11041 caacggtttg aatcattatc tggccgataa accgacggtg atggcagcga tgaagcagaa
    11101 aaccgggttg aggtaattat tccgggtgtc gccggatgcg gcttgagcat ccggcaccac
    11161 aaaacgttta cttaaccatc tgctccagct tttcgcgcaa ttccccttcc agagctaatg
    11221 ctttctgcgt tttaagatca atacaaacaa acgtaataag cgcatccgct accacctgcc
    11281 cttccggctc cagtgtaatg acctggttta agatgccgct tttaccgttt aattgctgca
    11341 actgactggt aatagttaac aggtcgctta ataccgctgg acgacgatag ttaatattga
    11401 tattgaccac gacgaaggcg atgttatggg ccgtcatcca ctgaaaactg tcgctatttt
    11461 ccaccccatc ccagcgggct tcttcgagaa attcaaggta gcgggcgttg ttgacgtgct
    11521 ggtaaacgtc gagatgatat ccacgaactt tgatttgtgt ttgcatagcg caataacctt
    11581 acgttgttgt tataagtaca gaggtcataa ctggtatgac ctcttcagtc tggcaaaaaa
    11641 ttgccactat gcaaattaat tacagggtta ataccgccag attacgttcc accagcgaat
    11701 tgcccatccc cggcacctgc tttagatcct ccaccgtttt aaacggaccg tactcttcgc
    11761 gataactgac aatcgcctgc gctttcttca ggccaacgcc attcatcgcg cgggctagct
    11821 cttccgcgct ggcattatta atgctgaccc gggtgccttc ttcgtcactg gctttcgccg
    11881 gtactgctgc tttactttgt gctgcaggag cttccgcttt ggtttctacc gccgtcggtt
    11941 tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc agggacagtg
    12001 taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg ttaacagtgg
    12061 aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa tggaaaaggc
    12121 cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg cggcttcagg
    12181 attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc aggttactca
    12241 tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc atcgcttttt
    12301 tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga accacattac
    12361 cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt ggcagtgcaa
    12421 acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc tcgccaaatt
    12481 tcagaccggc agcctgcata gcttccgcac ctttgccggc tttcaaatca accagcagtt
    12541 tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt gccttaactt
    12601 gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg acgcgcagca
    12661 cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccgac gcgccgtttt
    12721 cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc aactcttccg
    12781 gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca gcttgctctg
    12841 caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc tgcagcgcat
    12901 agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca cgtacttcgt
    12961 ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg aaaccgaccg
    13021 aagatttgat gacaccagac agttggcctt tttctttcag accagcattt ttcagttcgt
    13081 cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca gagataatat
    13141 cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc tcatcaagta
    13201 ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta cgctgcggct
    13261 gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg ctaaccggtt
    13321 gctgcatcgt tgcggcaacc agcttgatgt aactcacgcg gaattgttcc ggcgtcatga
    13381 aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca ggctgcttcg
    13441 ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc gcgaccagtg
    13501 ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaacg ccgttaatca
    13561 gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg gtcatcccca
    13621 tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc tggaaggctg
    13681 gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc agctcacgag
    13741 cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga cgcagggttt
    13801 tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt tgctgcatac
    13861 gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc tggtcattca
    13921 cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg gtcaatatga
    13981 acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca gccgtgcgta
    14041 agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct cacgcaacgt
    14101 atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag cccacaagac
    14161 acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc ttgaacttcg
    14221 tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt tacctgcacg
    14281 gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg ggttgcggcc
    14341 agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg ctacatcatc
    14401 cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac ggccagccgc
    14461 agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt tattcactct
    14521 tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa cgcgcagcag
    14581 ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc cgatccgcta
    14641 tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca gccctgtttt
    14701 tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg ccagacggtt
    14761 cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga atgtccagat
    14821 cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac ggaattagca
    14881 ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca ccgatcggca
    14941 gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga accgggttac
    15001 cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga ccatctttcg
    15061 gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa tcagggttga
    15121 tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata gactcctgca
    15181 tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca catgcggttt
    15241 caatggtcag caagtcaccg cccacttccg tccacgccag accgattacc tgaccgacac
    15301 ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag tcatgcaggt
    15361 tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc ttaaccgctt
    15421 tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca cgggtgtagt
    15481 aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt ttcagtgcat
    15541 tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt tcatcttcgg
    15601 tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga atgttcatgg
    15661 agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc aggtagtggt
    15721 cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag gccggatcgc
    15781 ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcgga tttttcacgc
    15841 ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa gtacggcggt
    15901 gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca tatttacgcc
    15961 cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc ggccctacca
    16021 ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg tactcaagga
    16081 tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc tgcgcctgac
    16141 gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc cagtcgatat
    16201 aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc ttctgcaact
    16261 ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt ttgcgcttca
    16321 gggcttcgtt ttcgtccggc acgtcgtcca tttcaccgag ttctttctga atagctttca
    16381 tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta acgcggttgc
    16441 gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc atcagatatt
    16501 ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc agtttcagcg
    16561 gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta ttcagcgacg
    16621 tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac tggctgattg
    16681 cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc agatactccg
    16741 ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg cgctgtaacc
    16801 cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat atagaggcca
    16861 cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct tctttctgcg
    16921 cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg atagattttt
    16981 cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg cgcagcggca
    17041 atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct cttagtttaa
    17101 tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa catgtacgtc
    17161 agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa ttaaagggga
    17221 gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta ttcaccagat
    17281 gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc atcaattacc
    17341 gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta catggtatcg
    17401 agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg cgccatcgct
    17461 ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc ttccagatta
    17521 aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat ctgaatcaga
    17581 gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat gaactcaggg
    17641 ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc gccttcgctt
    17701 gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc ggtttctaca
    17761 cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa caggatctta
    17821 gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg cggtggaaca
    17881 gcagctaccg taccttcgat cagtttcaac agtgcctgct gtactccttc accggaaacg
    17941 tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc atcgatgtag
    18001 acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag cttctgaatg
    18061 atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc gtcggccatg
    18121 gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt accggaaccg
    18181 gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct ggtatcgccg
    18241 ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt cgcctgttcc
    18301 tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg tagcgcactg
    18361 cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt acataaatca
    18421 acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac ttcatgctgg
    18481 cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg tttatctgtc
    18541 atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa tgccattctc
    18601 aggcgcaagc cgcttacagc gttgtgccgc cctagataag tatagcggca cagttgcgcc
    18661 tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc accgcttcag
    18721 gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat gattgacccg
    18781 tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca cgggcatgaa
    18841 tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc atcacgcgcg
    18901 aattcggcag gcaaaaacgt ttaccttttg cccctgcggt caacaagaaa gcgcccatcg
    18961 aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc atggtgtcat
    19021 agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga tagatatctt
    19081 tttctgggtt ttccgcttcc aggaacagca tctgcgccac aatcaggtta gccatgtggt
    19141 cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa tagatatcaa
    19201 aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc atatggggtg
    19261 caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa ttgaaaaaaa
    19321 cctgctgtac cgattgtaac cttatggacg gattatcagc tagtccctgt gttatgggta
    19381 cggcatcacc atatttcaag cataacaatc ttttgttgtt acgctaacac cgaaagaggc
    19441 ttttcgcacg gctccatgca aaattcatga caaaaaaaag cccaccacct ggcggtgacg
    19501 ggcctttgtg cgaatttagc gcgttatgct gcgtaaatta cgcctgctgg ttcatcagct
    19561 cgttgaaagt ggtttctttt tcagtcactt tcgctttcgc cagtacagct tcaacagcct
    19621 gttcttccag agcaacattg cgcatgttgt ccatcagttc tttgtttttg ctgtagaact
    19681 cgataacttc tttcggatct tcgtacgcag aagccatctc ttcgatcagg cctttcacgc
    19741 gctcttcgtc agctttcagc tcgttggtgc ggataacttc gcccagcagc aggccaacaa
    19801 ctacgcggcg tttagcctgt tcttcgaaca gttcgcgcgg cagttccaga gcttgttttt
    19861 cgttgccacc gaaacgctgt gcagcctggc gacgcagaac gtcgatttcg ctgtcgatca
    19921 gcgcagccgg tacgtcgatg tcgttagctt ttaccagacc ttcgatcgcc tgagacttaa
    19981 cgcggttacg gatggcgctc ttcagctcgc gctccatgtt tttacgcact tcagcgcgca
    20041 gaccttctac ggaaccatct tcaacgccga aacgtttgat gaattctgca gtcagttccg
    20101 gcagttcacg ctcttcaact ttcttcaggt tgatagcgaa tttcgctgct ttacctttca
    20161 ggttttctgc gtggtattct tccgggaagg tcacgtcgat ggtgaactct tcgccagctt
    20221 tgtggccttt gataccgtct tcaaagcccg ggatcatacg accctggccc atcgccagta
    20281 cgaaatcaga cgctttaccg ccttcgaact cttcgccgtc tacagaaccg gtgaagtcga
    20341 tggttacgcg gtcttctgct tcaacagcgc cgtctttttc tttccaggtc gcctgctgtt
    20401 tacgcagagt atccagcatg ccgtcaacgt cagcgtcggt cacttcaacg atcggttttt
    20461 caacttcgat cgcttccaga ccctgcagtt caacttccgg ataaacttca aactctacag
    20521 agtaagtgaa gtcttcaccc agcttgtatt cgcccggaac ataagtcggt gcgccagccg
    20581 gattgatttt ttctttaatg atggcgtcaa tgaagttacg gctcatcagg tcacccagaa
    20641 cgtcctggcg tacagacgcg ccataacgct gagcaacgat attcattggc actttgcctt
    20701 tgcggaagcc gtcaatacgt acttttttcg caacgttgac cagctcgctt ttaacagcgg
    20761 tctcgatgct gtcagcagcg atagtaatcg ttacacggcg gccaaggcct tgagtggttt
    20821 caactgaaac ttgcatcttg ttacctcaaa aaatcacagt gctcggtcaa ctcttacccc
    20881 gcaagcacag gttgttggct tcgcggaacc gggatgttct tataatcaat cacattccct
    20941 gttaccagaa tcatcccgaa agcattcaaa taggacgcgg cattatagcg gcatcacttt
    21001 tatgagtcga gaacggttat tgcgcgctgt cgcgtatttt ttcacaattc ccgggcaatt
    21061 ttcgtctgaa tgctgggcaa aaagcgcaaa atccagacaa aacaaaacgg cccgcaggcc
    21121 gtttttcata aaatctttag caacatactg gaaaagcgcc gacagttgca aatgcgtttt
    21181 tacgcgatgc ttcctgctcc acgacaggga ggagaggcaa agacggtgtc ctgcaaccct
    21241 tcccactcct taatagtgta agtatgcaga gccagcgcat gaacggtagt agagagttcc
    21301 tccgctaaag tactgtaaat cattcgatga cgattcagaa aacgttcacc cgtaaaacga
    21361 tcgctgacca gcacaacttt aaaatggctt tcagagccgg ctgggacatt gtgacgatag
    21421 ctttcatcca ctacttcgag gaatacgggt tggaacgccg cccttaattt ttcttctatc
    21481 cgctcacgta tcatcatgaa atatctcctc cgacaacgct gagatgtcat ccatcccttt
    21541 aaatactagc cgcttttacc gtttccattg cagcttaaca acaaatattt aggtttcgtc
    21601 ctggtttttc cgtcaagcgt atgaagttta ctgatttaaa gattgaaaca caggcctcgc
    21661 atcagcgttt cttttaccag gggtgattat ttcgtcaaca tgatgaattt acatgcctta
    21721 cccacttccc ctcgccgttg gcaatgttat gatggcggga atttttacca aacacgctta
    21781 agagtctctg agagcccgaa catgttcaaa aaaatcctct tcccgttagt tgctctgttt
    21841 atgcttgcag gatgcgcaaa accgccaaca actattgaag tttccccgac gattacactg
    21901 ccacagcagg atccaagcct gatgggcgtc accgtaagca ttaatggtgc cgatcagcgt
    21961 accgatcagg cgctggcaaa agtcacccgc gataatcaaa tcgttaccct gaccgcctcc
    22021 cgcgctctgc gtttcctgct gcaagaagtg ctggaaaaac agatgaccgc gcgtggttac
    22081 atggttggtc cgaatggccc ggttaatctg caaatcatcg ttagccaact gtatgctgac
    22141 gtgtcccagg gcaacgtgcg ctacaacatc gctaccaaag cggatatcgc catcatcgct
    22201 accgcgcaga atggcaacaa aatgaccaaa aactatcgtg ccagctacaa cgttgaaggt
    22261 gcgttccagg cctccaacaa aaatatcgcc gatgcggtta acagtgtgct gaccgatacc
    22321 atcgctgata tgtctcagga caccagcatt cacgaattca tcaagcagaa cgcgcgttaa
    22381 tttctgccct ctggcccggt gcaagccggg cctgtagacg cccatgtcca gtcaatattt
    22441 acgtattttt caacagccgc gttcagccat attgctgatc ctgggttttg cttccgggct
    22501 accgctcgcc ctgacatccg gcaccttaca ggcctggatg acggtcgaaa atatcgatct
    22561 caaaaccatt ggtttcttct ctctggtagg ccaggcttac gtttttaaat tcctctggtc
    22621 accgctgatg gaccgctaca cgcccccatt ttttgggcgg cggcgcggtt ggctgctcgc
    22681 cacgcaaatc ctgttattag tcgccattgc ggcgatgggt tttctcgaac caggcaccca
    22741 actccgctgg atggcggcgc tggcggtggt gatcgctttt tgctctgcct cccaggatat
    22801 tgtcttcgat gcgtggaaaa ccgatgtgct cccggcagaa gaacgtggtg cgggcgcggc
    22861 aatcagcgtg ctgggttacc gtttagggat gctggtttcc ggcggcctag ccctgtggct
    22921 ggcagataaa tggttgggct ggcagggcat gtactggttg atggcggcgc tgttgatccc
    22981 ctgtattatc gcgacgttgc ttgcaccaga accaaccgac accattcctg tgcccaaaac
    23041 gctggaacaa gcggttgttg cacctctgcg agatttcttt ggtcgcaata atgcctggct
    23101 tattttgctt cttatcgtgc tgtataagct gggcgacgca ttcgccatga gcctgacaac
    23161 cacgtttttg attcgcggcg tcgggtttga tgcgggtgaa gtaggcgtgg taaacaaaac
    23221 gcttggctta ctggcgacca ttgttggcgc attgtacggt gggattttga tgcagcgcct
    23281 gtcgctgttc cgggcgctgc tgattttcgg cattttacaa ggtgcgtcta acgctggtta
    23341 ctggctgctg tcgattactg ataagcatct ctacagcatg ggcgcagccg tctttttcga
    23401 aaacctctgt ggcgggatgg gcacatcagc ctttgtcgcg ctgttaatga cgctgtgtaa
    23461 taagtcattt tccgctactc aatttgccct gctctcagcg ctttccgctg tagggcgagt
    23521 ttatgtcggc cccgtggcgg gttggtttgt tgaagcacac ggctggtcga cattctatct
    23581 attctccgtc gccgctgccg taccggggct tattttgctg ctggtttgcc gccagacgct
    23641 tgaatataca cgagtaaatg acaactttat ctcccgtacc gaatatccgg caggttatgc
    23701 ctttgccatg tggacactgg cggcgggcgt cagcctgttg gccgtgtggt tacttctgtt
    23761 gacgatggac gcgctggatt tgacgcactt ctctttcctg cctgctctgc tggaagtcgg
    23821 ggttttagtc gccctttctg gcgtcgtgct tggtggtttg ctggattatc tggcgctacg
    23881 aaaaacgcat ctgacgtaat ctgtaaatat tatttagctg tagtaatcac tcgccgtaat
    23941 tattacggct aaataaacat cagcatcgct tattaatatt tgttcttttg tgcggcttag
    24001 cgtttggaaa ttattgacgc catttataaa ttctattttt cttacacgat tcagctaatg
    24061 agtctttatt ttctcaccac ccagttgtca ctttaatgat aattatttgt ta
//
LOCUS       DAZSNO010000049        23209 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZSNO010000049 DAZSNO010000000
VERSION     DAZSNO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23209
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.049"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..246
                     /locus_tag="ACHWN2_001891"
     CDS             <1..246
                     /locus_tag="ACHWN2_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR8931449.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHWN2_001892"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHWN2_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR8931450.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHWN2_001893"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHWN2_001893"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR8931451.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHWN2_001894"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHWN2_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFR8931452.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHWN2_001895"
     CDS             complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHWN2_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR8931453.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHWN2_001896"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHWN2_001896"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR8931454.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="ACHWN2_001897"
     CDS             complement(4442..5650)
                     /locus_tag="ACHWN2_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8931455.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHWN2_001898"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHWN2_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR8931456.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="ACHWN2_001899"
     CDS             complement(6445..8130)
                     /locus_tag="ACHWN2_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR8931457.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="ACHWN2_001900"
     CDS             8400..8777
                     /locus_tag="ACHWN2_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR8931458.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHWN2_001901"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHWN2_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR8931459.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="ACHWN2_001902"
     CDS             9224..9511
                     /locus_tag="ACHWN2_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR8931460.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHWN2_001903"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHWN2_001903"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR8931461.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="ACHWN2_001904"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="ACHWN2_001904"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR8931462.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="ACHWN2_001905"
     CDS             11268..11744
                     /locus_tag="ACHWN2_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR8931463.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="ACHWN2_001906"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="ACHWN2_001906"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR8931464.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14436
                     /gene="potG"
                     /locus_tag="ACHWN2_001907"
     CDS             13303..14436
                     /gene="potG"
                     /locus_tag="ACHWN2_001907"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR8931465.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14446..15399
                     /gene="potH"
                     /locus_tag="ACHWN2_001908"
     CDS             14446..15399
                     /gene="potH"
                     /locus_tag="ACHWN2_001908"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR8931466.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15396..16241
                     /gene="potI"
                     /locus_tag="ACHWN2_001909"
     CDS             15396..16241
                     /gene="potI"
                     /locus_tag="ACHWN2_001909"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR8931467.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16301..16789
                     /locus_tag="ACHWN2_001910"
     CDS             16301..16789
                     /locus_tag="ACHWN2_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR8931468.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16830..17957
                     /gene="rlmC"
                     /locus_tag="ACHWN2_001911"
     CDS             16830..17957
                     /gene="rlmC"
                     /locus_tag="ACHWN2_001911"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR8931469.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="ACHWN2_001912"
     CDS             complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="ACHWN2_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR8931470.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19155..19823)
                     /gene="artM"
                     /locus_tag="ACHWN2_001913"
     CDS             complement(19155..19823)
                     /gene="artM"
                     /locus_tag="ACHWN2_001913"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR8931471.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="ACHWN2_001914"
     CDS             complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="ACHWN2_001914"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR8931472.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20546..21277)
                     /gene="artI"
                     /locus_tag="ACHWN2_001915"
     CDS             complement(20546..21277)
                     /gene="artI"
                     /locus_tag="ACHWN2_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR8931473.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21295..22023)
                     /gene="artP"
                     /locus_tag="ACHWN2_001916"
     CDS             complement(21295..22023)
                     /gene="artP"
                     /locus_tag="ACHWN2_001916"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR8931474.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22241..22756)
                     /locus_tag="ACHWN2_001917"
     CDS             complement(22241..22756)
                     /locus_tag="ACHWN2_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8931475.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDTRNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         5694 a         5735 c         6001 g         5779 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc tccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg tatgctggca
    13501 ggtttcgaac aaccttctgc cggacagata atgcttgatg gcgtcgattt gtcacaggtt
    13561 ccgccttacc tgcgccccat caatatgatg tttcagtctt acgcgctgtt tccgcatatg
    13621 accgtggaac agaacatcgc ttttggcctg aaacaggaca aactaccgaa agcggaaatt
    13681 tccagtcggg tcaatgagat gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa
    13741 ccgcatcagc tttctggtgg tcagcgacag cgtgtggccc tggcccgaag ccttgccaag
    13801 cgcccgaaac tattactgct cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac
    13861 cggatgcagc ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc
    13921 acccacgatc aggaagaggc gatgaccatg gcggggcgca tcgccattat gaatcgtggg
    13981 aaatttgtcc agattggcga accggaagag atctacgagc atccgactac ccgctatagc
    14041 gccgaattta ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat
    14101 ggcttggtgc ttgattcgcc ggggctggtg catccactga aagtcgacgc ggatgcctcg
    14161 gtggtcgata acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa
    14221 gagccgcccg ccaatggttg taacttcgcg gtgggggagg tgatacacat tgcctatctc
    14281 ggcgatcttt cggtgtatca cgttcgtctg aaaagtgggc agatgattag tgcccagcta
    14341 caaaacgccc atcgtcatcg taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc
    14401 tgggaagtgg acagctgtgt ggtgctgacg gtttaaggag caaagatgaa tacacttgaa
    14461 cctgctgccc agtcgaaacc gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg
    14521 aagcatgggc gcaaactggt cattgcgttg ccatatatct ggttgatctt gctgtttctg
    14581 ctgccatttc tgattgtctt taaaataagc ctggcggaga tggcgcgcgc tattccacct
    14641 tataccgaac tgatggagtg ggctgacggg caactgtcca tcactcttaa tctcggtaat
    14701 tttctgcaac tgaccgacga tccgctctat ttcgatgctt atctccagtc gctacaggtg
    14761 gctgcgattt cgacattttg ctgtttactg atcggctatc cgctggcgtg ggcggtggcg
    14821 cacagtaagc cttcgacccg taatatttta ttactactgg tgatcctgcc gtcgtggacc
    14881 tcgtttctga tccgcgttta tgcttggatg ggaatattaa aaaacaacgg tgtgctgaat
    14941 aattttctgc tgtggctggg ggttatcgat caaccgctga ccattcttca taccaatctg
    15001 gcagtttata ttggcattgt ttacgcctac gtgccgttta tggtgctgcc gatttatacc
    15061 gcgttgattc gtattgatta ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg
    15121 ctgaaaacgt tctttactgt gatcgtgccg ctgactaaag gtgggattat tgccggatcg
    15181 atgctggtgt ttatcccggc tgtgggcgag tttgtgatcc cggaactgct cggtggcccg
    15241 gacagcatca tgatcgggcg cgtgctatgg caagagtttt tcaataaccg cgactggccg
    15301 gtggcctcgg cggtagcgat catcatgttg ctgctgctaa ttgtgccgat aatgtggttc
    15361 cacaaacacc agcaaaaaag cgtgggagaa cacggatgaa taatttaccg gtagttcgtt
    15421 cgccctggcg gattgtgatt ttgctgctgg gctttacctt tctctacgcg ccaatgctga
    15481 tgctggttat ctattcgttt aacagctcga agctggtgac ggtgtggggc ggctggtcaa
    15541 cgcgctggta tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc
    15601 tgacaattgc tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt gcggcggtgg
    15661 tgctggtgcg ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc
    15721 cgctagtaat gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg
    15781 ctcatgccat tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca
    15841 ctttctgtac ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct
    15901 cgatagaaga agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta
    15961 cgctaccgat gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc
    16021 ttgatgatct ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc
    16081 tggtcttttc cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga
    16141 tcctcggcgc ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa
    16201 aacagcggat acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa
    16261 cctggctaca taatgccgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg
    16321 attttttaag aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt
    16381 ggcggcgctc gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct
    16441 gggcgtgcgc ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct
    16501 ggtcttttta agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt
    16561 gaaagggcgt cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta
    16621 cttgtgggcg gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc
    16681 aaaacgtgaa atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat
    16741 gctgctgttc gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc
    16801 gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct
    16861 gtcgttcctg tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg
    16921 atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc
    16981 cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac
    17041 tgctcggtat gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc
    17101 cagcctcatt tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa
    17161 cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc
    17221 agagtgatgg cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac
    17281 tgcgtaaggc gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca
    17341 atattcagcc ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac
    17401 aacaggcact ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct
    17461 tccagactaa tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac
    17521 agctgccggt taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact
    17581 gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg
    17641 caaagcagtc agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca
    17701 ctcagtttgc cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc
    17761 gcggcattgg taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct
    17821 actccagctg taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca
    17881 tcgaacgggt acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc
    17941 tgctggtgaa gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt
    18001 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat
    18061 acattgcaga gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc
    18121 atcagtctca attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct
    18181 ttaattgctg ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca
    18241 gcgataccca ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc
    18301 agttgcggat tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca
    18361 atacgaccat ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc
    18421 acttccgggt gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg
    18481 cgtttgcctt tcaggtcggc aaacgttttg taggtatctt ttttggaaat cacgacggct
    18541 gagttttcat agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc
    18601 ataccggaga ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac
    18661 gcgtgattag taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg
    18721 atatcaaagc cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct
    18781 gaaacgccaa aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa
    18841 gcggcaagaa ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc
    18901 cgtgtctgcg gcatgggata acaatgccat caagtgaatt tatatgcaat aaacatgatt
    18961 aaataattta aatgaaataa aaaaagacgg ataacttagt gagttgtccg tcttcattat
    19021 aagaatttat gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc
    19081 gtgctgatgt ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa
    19141 tgtacgcagc tacattaatt ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc
    19201 atcatcagcg tcagcaggcc gttgacgacc aggtaaataa tccctgccgc accgaacacc
    19261 attacatcgt aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta
    19321 atggtgtaag ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag
    19381 agcgagcgtt taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc
    19441 agggcgctac aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc
    19501 tgcgtggtat acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct
    19561 gacaacaaat gccacagtgc cggatactcc tgcaacgtcg gaaactggcc cggcccgtaa
    19621 taaatcagga agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc
    19681 accagccaca ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga
    19741 atcagtgcca caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc
    19801 agctcgggta aatactcaaa cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt
    19861 caatgcgttt gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca
    19921 caatgtacca ggtaaatggt tcctgggtac gagtagcaat gcttttggtt tgtaacatta
    19981 aatcattcac actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt
    20041 taccgaggcc aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag
    20101 ccgatttcga cagccccagc gcctgaccag attcccactg gcccaccggt acagctttca
    20161 acgcgccgcg cagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac
    20221 cacaaaggaa cggactcacg tcgaagttct caatgtccat ctgcaccggg atctgcacga
    20281 acccaagatt gatagtgaag ccatccgaaa gcgtcagcag taactgcgag gagccaaaat
    20341 agataaacag cactaccaga atttctggca ggccacgcag aatggttacc agcgctgaac
    20401 ctgcccacgc gacaggacgc catttcgctg actcccatac cgcaaagaac atcgccagcg
    20461 ccagcccgac aatcaatgca caaacggcaa ggccgacggt catcccggcg gcgcttgcta
    20521 aaggaaaaaa ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt
    20581 aagtgccatc tttcttcact ttttccagcg cagtgttgag tttctgctgc agttcagtgt
    20641 ttccctgacg taccgcgatg ccgaggccag taccgaaata atctttatcg gtcactttat
    20701 cgcccactgc cgccagtttt gggttatctt tcagccactc agtgaccact gcggtgtcac
    20761 caaatacgct gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat
    20821 acggaacggt ggtgatttcc gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt
    20881 tctgaacgcc gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac
    20941 ccacaaacag ggcagagttg tcatagtacg gggtagtaaa cagtacctgc ttttcacgct
    21001 ccggagtgat atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga
    21061 tcaggctgtc aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg
    21121 cctgcgccag gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg
    21181 gaggataggg ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa
    21241 aacctgcaat taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg
    21301 agagagatag tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg
    21361 ttctacgata tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc
    21421 aacttcgtgg gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct
    21481 gacgatttgt gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac
    21541 ctgcggttcc atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag
    21601 atgcagcggg taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc
    21661 acgggccagc gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt
    21721 ttgctgcacg gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg
    21781 acgcaaatcg cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc
    21841 aatgttgagc gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct
    21901 tttacccgcg ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt
    21961 gatatcgaac agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact
    22021 cattgacact cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttgac
    22081 agaataatta tgcaatattt atgctttaaa agttaaaagc aaagcgcatt attcaataaa
    22141 catagcacaa aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa
    22201 atgccggata atttacgggg tttattggtt gatcaaggcg ttagcgattc tcgatggact
    22261 gacggagcgt acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga
    22321 cccaacactg gccttcctga atcattaaca cttcatcctg ccagccctgg tcaccctgtt
    22381 tgagatccac ccgcaatgga atgttacggg tatcacgatt agggatagtc gatgcactgg
    22441 caacgtgggc gctgtctggc aaggtggttc gactggagaa cggatcgctg cttaacaatt
    22501 cacgatgact attatcgcgg ctggcatcgc taagttgggt tgccagtttg tcactcaaat
    22561 aggggcgcag ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg tagaattgtt
    22621 gagccacact gtcaggccct ccctggacac aggaaccggt gcgggggccg ttatctttat
    22681 aagctggcgt gacggtggta caggcactca gcagcagtgc acatggaata agtagagata
    22741 gtgaacggta gcgcataatc atttccttat tagcttgctg tctgcgtcac actacgacca
    22801 aacgctatca gattatttaa tacattgttt ttaatacaat tctgaaacaa tctgaacacc
    22861 tgattgggtt tgagtccatt gctggcgacc cactcaaggt tgctcgtagc tgacttactt
    22921 aaattaccca aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt tatatggctg
    22981 aaatgatcat ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc ctcctgtaga
    23041 ggttaagagc atctgcaaga agccagtttc acatttataa cgcgtgataa atgaatgcgg
    23101 ttattgtcta atcattacaa aatttttcac atgcgataag agatcctgcg attaagatga
    23161 ttgccaaaat tacgttgtat ttttgaaaat gagcgaaatt tgatagtcc
//
LOCUS       DAZSNO010000050        22691 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZSNO010000050 DAZSNO010000000
VERSION     DAZSNO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22691
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.050"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            12..1034
                     /locus_tag="ACHWN2_001918"
     CDS             12..1034
                     /locus_tag="ACHWN2_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFR8931476.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1034..2014
                     /locus_tag="ACHWN2_001919"
     CDS             1034..2014
                     /locus_tag="ACHWN2_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR8931477.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2011..2769
                     /locus_tag="ACHWN2_001920"
     CDS             2011..2769
                     /locus_tag="ACHWN2_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8931478.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            2779..3588
                     /locus_tag="ACHWN2_001921"
     CDS             2779..3588
                     /locus_tag="ACHWN2_001921"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8931479.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            3588..4442
                     /gene="modD"
                     /locus_tag="ACHWN2_001922"
     CDS             3588..4442
                     /gene="modD"
                     /locus_tag="ACHWN2_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFR8931480.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            4468..6422
                     /locus_tag="ACHWN2_001923"
                     /pseudo
     CDS             4468..6422
                     /locus_tag="ACHWN2_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(6472..6726)
                     /locus_tag="ACHWN2_001924"
     CDS             complement(6472..6726)
                     /locus_tag="ACHWN2_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR8931481.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6927..7661
                     /locus_tag="ACHWN2_001925"
     CDS             6927..7661
                     /locus_tag="ACHWN2_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR8931482.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7663..8274)
                     /gene="emtA"
                     /locus_tag="ACHWN2_001926"
     CDS             complement(7663..8274)
                     /gene="emtA"
                     /locus_tag="ACHWN2_001926"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR8931483.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8374..9288
                     /gene="ldcA"
                     /locus_tag="ACHWN2_001927"
     CDS             8374..9288
                     /gene="ldcA"
                     /locus_tag="ACHWN2_001927"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR8931484.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            9383..11119
                     /locus_tag="ACHWN2_001928"
     CDS             9383..11119
                     /locus_tag="ACHWN2_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR8931485.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            11730..13144
                     /locus_tag="ACHWN2_001929"
                     /pseudo
     CDS             11730..13144
                     /locus_tag="ACHWN2_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13312..14382)
                     /gene="dadX"
                     /locus_tag="ACHWN2_001930"
     CDS             complement(13312..14382)
                     /gene="dadX"
                     /locus_tag="ACHWN2_001930"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR8931486.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(14392..15690)
                     /gene="dadA"
                     /locus_tag="ACHWN2_001931"
     CDS             complement(14392..15690)
                     /gene="dadA"
                     /locus_tag="ACHWN2_001931"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR8931487.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16020..17553
                     /locus_tag="ACHWN2_001932"
                     /pseudo
     CDS             16020..17553
                     /locus_tag="ACHWN2_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
     gene            complement(17605..18324)
                     /gene="fadR"
                     /locus_tag="ACHWN2_001933"
     CDS             complement(17605..18324)
                     /gene="fadR"
                     /locus_tag="ACHWN2_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR8931488.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            18545..20086
                     /gene="nhaB"
                     /locus_tag="ACHWN2_001934"
     CDS             18545..20086
                     /gene="nhaB"
                     /locus_tag="ACHWN2_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR8931489.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20232..20762
                     /gene="dsbB"
                     /locus_tag="ACHWN2_001935"
     CDS             20232..20762
                     /gene="dsbB"
                     /locus_tag="ACHWN2_001935"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR8931490.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(20808..22076)
                     /gene="umuC"
                     /locus_tag="ACHWN2_001936"
     CDS             complement(20808..22076)
                     /gene="umuC"
                     /locus_tag="ACHWN2_001936"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR8931491.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22076..22495)
                     /locus_tag="ACHWN2_001937"
     CDS             complement(22076..22495)
                     /locus_tag="ACHWN2_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR8931492.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         5413 a         5982 c         5692 g         5604 t
ORIGIN      
        1 ggatgttcac catgcccata accagacgga cgtttgcgca ggcacttgca tctaccctgc
       61 tcctgcagtc tctaccgtcc ttttcacaaa cagttaatcg ttttgcttcg caatctcttc
      121 ccgaagccca aaacattaca cgcatcgtca gcgcaggcgc gcccgccgat ttgctcttgc
      181 tggctgtcgc gccagaaaaa atggtcggtt tttcctcttt tgattttgcc cgtcaggcat
      241 taattccatt gccagagcac attcgccagt tacccaggct gggacgactc gccgggcgcg
      301 ccagcacact atcgctggaa gggcttatgg cgttacatcc cgatttggtc gttgattgcg
      361 gcaatacgga tgaaaccttg atctcccagg cacggcaggt tagcgaacag acacaaatcc
      421 cctggttatt gcttaacgga aaactggcgc aatcagcaga acagttaaca acgcttggca
      481 aaacgttagg cgaagagcac cgcgccgcag aacaagccaa tctcgccagc cacttcgttg
      541 gtgaagctca ggcattcgcc acctcacccg ccgctaacct ccgcttttat gctgcgcgcg
      601 gtccccgagg gctggaaacg ggcttacagg gttcgttgca taccgaggcg gcggaattat
      661 taggtttgca caacgtcgcg caaatagccg atcgccacgg cctgacacag gtttccatgg
      721 aaaatctcct gcgctggcag ccggatatta ttctggttca ggaggccgtt actgcagatt
      781 ttattcgtcg tgatcctctc tggcagggcg tgaaagccgt tgcggaacaa cgcatcttat
      841 ttttaagtgg cttgcccttt ggctggctcg atgccccgcc gggaatcaac cgtcttctgg
      901 gattacgcag acttcacgcc tggctggatc ccgccatcaa tcgccagttt aaaagtgaca
      961 tgcagcatta cgcccaactg ttctggcatt gttcactcag tgatgccgac tatcaaaaat
     1021 tggtggcgag ctaatgagaa tcgtaaacgg atgtattttg ctgctggcag ctatcagtat
     1081 cacttttgcc gccgtaagtg gggcttatca tctcgatatg cagcaactgc tggcgctaat
     1141 tcttcgtcaa gaaaatgagt ctgtgcagga acaaattgtc ttctggcaaa tccgcgtgcc
     1201 gcgtattctt gctgcgctgt tactgggcgc agcgctggca ggagccggaa ccacctatca
     1261 gggaatgttg cgtaatccct tagtttcacc ggatatttta ggcgtctccg ccggtgccgg
     1321 acttggtgcc tgcgcggcaa tcctgtgggg attacccatt gtttacatcc agctgtacgc
     1381 attttgcggc gggctgatgg tggtcgcggg cgtctggttg attacgcgtc gggtgacccg
     1441 ccacgatccg atcttaaccc tggtgctggt aggcattgcg ttaggaacgt tgtgcggagc
     1501 ggggatatcg ctaattaaaa cgctggccga tccttacacc cagttaccgt caattacctt
     1561 ctggttgctg gggggactgt ccaccgtgac cttacgcgat ctctgctatg ctgcaccgat
     1621 cattctcata ggctccttgc ccctgttttt tctccgctgg cgcatgaatt tactcacgct
     1681 ttccgacgac gaagcccgtt cgctgggact gaacgtgacg cgtctgcgct ttggattgat
     1741 tgtctgcgcc acactaatca ccgccagcac ggtagcaata gcgggcataa ttggttggat
     1801 cggtctggtc gttccgcaca ttgcccgctt gttgaccggt cataaccatc agcagctgtt
     1861 gcctatggca atgtgtactg gcgctattct gctcctgctt accgatacgc tggcgcgcag
     1921 cattggaaca acagaaatcc cacttggcat tctgaccgct tttgttggcg caccgttttt
     1981 cctgcttttg ttgttacgcg gaggtcgcca gtgactttgc ttgccgttcg tcatgcgtct
     2041 ctgggttact cccgccatcc agtgctacgc gacgtgtcct tcaccctgtc gcaaggaaca
     2101 atttgttgtc tgcttggcgc gaatggttgc ggcaaaacca cactcatgcg ctccattctt
     2161 ggcgttatac cgttattaaa aggcgagatc ctactggata gcctctctgt gcagacactc
     2221 agccatcgcc agcgcgcaca ggctattgcc tgggtgccgc aggcgcacga cggtattttt
     2281 gctttcagtg tgctggatat ggtgttgatg ggcctggccc ccaccatagg cgctttttcc
     2341 gttcccggta agcaagaacg cctgaaagcc acggagcaac tggaaaagct gggcattttg
     2401 catctggcga cacgacgctg gaatacgctt agcggcggtg aacgtcagct ggctttaatc
     2461 gcccgtgcac tcgtccaaca accgcgcttg ttgttactgg atgagcccgc ctccagcctc
     2521 gatttcggtc atcagatcca acttctggac acgctggcgc agctcaaaaa taacggcatg
     2581 acaatgttaa tgtcgactca ccatacgctc catgccaacg ccattgccga cagcattatc
     2641 caagtagagc cagatggacg agtaacacaa ggcttaccga ccgaacagtt aacaacgaac
     2701 aaactggcgg ccctgtatcg ggtttctgcc gaccaaattc atcatcacct ctctgcaatt
     2761 agccgctaag gaacaatcat gctcattgat gatattgatt ttgcagacct ttatctgcag
     2821 caactcaaac tggctcaccg cacagaaaaa acgcccgatc actgggatca acgggcggag
     2881 aaaatggcgg aaaattgcgc ctcgccaacg gacagctacc tgcaacaact gatcgctaaa
     2941 attgacctgc aaggcgctca gaccttattc gatatgggct gcggaccagg caccgtatcg
     3001 ctggcgctgg ctgacaaact cactacggtt tatggcgttg attacagtca ggggatgctg
     3061 aacgttgccg cccgccgcgc cgccgcactg aaggctgaca atgtccactg gatccagcgc
     3121 gcctgggaag aggactggag tgacctgcca cgctgcgata ttgccgtggc ttcccgctcc
     3181 actctggtgg ctgatatgcg ccatgcaatg agcaaactga ataatcaggc acggcttcgc
     3241 gtctatacaa ctcatctggt cagcacctcg ttcgtctctc cagccattca gcgggcggct
     3301 ggtcgggaag tgatcgaatt acccaactat atctttgcgc tcaacgtcct gtatcagatg
     3361 ggaatttatg cccacgttga ttttatccgt ggacaaaatt gccaacagga taacagcacc
     3421 tgggaacgtt ttgagcagaa cgtcagctgg agtcttggcg cgcttaacga cgatgagcgt
     3481 gagcggttat atcactggta tcaacaacaa gatgctcgtg ccctcgcccc tgccagccgc
     3541 gactgggcgc tcatctggtg ggatagcgta ccgcaggagg cactgtgatg attttccttt
     3601 ctcaggcaca aatcgatgcg ttactgctgg aagatatcca gggaggtgac ctgaccacgc
     3661 gagcattaaa tattggacac cagcctggct atatagcgtt ttttcaccgt cagggcggct
     3721 gcgtcagcgg cgtttctgtc gcgtgtaaga tgttaactac gctgggatta actattgatg
     3781 acgcggtcag cgacggttca caagcgaacg cgggtcaacg gctaatccgt gcgcagggta
     3841 atgccgcagc acttcatcag ggatggaagg cagtccagaa tgtgctggag tggagttgcg
     3901 gtgtttctga ttatctcgct caaatgctgg cattgcttcg tgaacgttat cctgacggca
     3961 atatcgcctg cacccgaaaa gcaattccgg gcacccgctt actggcttcg caggcaattc
     4021 tggctgccgg aggactaatt catcgtgccg gatgtgcgga aaccatatta ttgtttgcca
     4081 accaccgcca ttttcttcat gacaatcagg gctggtcagg cgcaatcgct cagttacgtc
     4141 gccacgcacc cgagaagaaa attgttgttg aagccgacac gccgaaagag gctatcgccg
     4201 cgttacgcgc acaaccagac gtacttcagc tcgacaaatt tagtccgcaa caggcaactg
     4261 aaattgcgca aatagcgccg tcgctggctc ctcactgcac gctggctctg accggcggaa
     4321 ttaatctgac gacactcaaa aattacctcg actgcggcat tcgccttttt attacctccg
     4381 cgccttatta cgcggcacca gctgacatta aagtcagtct gcaacccaca gcctctattt
     4441 aattctttgt taaacggaat ttattctatg agactcaaaa aacgttatct ctgcacagca
     4501 ttatcacttg cctttaccca gcaggccgta gcggctcagg agagcgacac gctgaccgta
     4561 tggtccagtc cggtatcatc gacgacgacc accgttctcg atcaacccac catgaaggcc
     4621 ttggataaac agaatgtcgc tcaggcatta agtgtcgtcc ccggcgtggt gctgcaaaag
     4681 tcaggcagcc gcaacgaaga acaggtaaaa gttcgtggct ttgatagtcg gcaggtgccg
     4741 gtctatttcg acggtgtgcc catttatgtc ccctatgacg gcaacctcga tctggcgcgg
     4801 attctgacca acaatctggg ggcagtcgaa gtttccaaag ggtattcgtc gctgcttcat
     4861 gggcctaatc agatgggcgg agccattaat atcaccaccc tgaagaagca agtctgggat
     4921 atcgccaggg atggagccgt agtcaggaca atgcctacga tatgcatgct tcatttgccg
     4981 ccagcagcga tcttgggtat ttgcaagtca gcggtagcca gctaaagcag gattttctcg
     5041 gcctgccgca tggtgtaaat aatgatattg caggcaaaca cggcaagatg attaattcat
     5101 cggctgatga taaacgcggc attgtgaagc tcggttttac accgcgcgaa aacgatgaat
     5161 acacgctgac ttacattaag caggatggtg aaaaagataa cccgccatac agcggaaatt
     5221 gtggtcaaaa atcacgctac tggcagtggc cagagtatga caaagaaagt ttttattatc
     5281 agggagcgac ccaactaaac gaccgtttta ccctgaaaag tcggctgtat cgcgacacct
     5341 ttgaaaatac gctgatgatg tacaactcgc tggctgattt gaaaaataaa aaaggcagct
     5401 acagccatta ttccgattac agcgacggtg ccggattaca actggcagcc gatgtgcgtg
     5461 aaaacgatct gctgtcgttt gccgttaact ggaaagatga cgtacaccgg gaaaaaggtg
     5521 cgccgcacgc cgcttacgat cgctatgaag atcgtacctg gtcgctcgcc agtgaatatc
     5581 aatgggctgc tgctgataat gtcgatgtcg tggcgggaat cagctatgac tggcgcgata
     5641 gcgtagaagc gaaaaaacac gagaaagatg gcagcatcac ccattatgac gacaacaatc
     5701 agtcagcttt taactggcag gtgatgggga aataccactt tgccaatgaa gacactctgg
     5761 cgctttcgta ctatgaccgc acacgctttc cgacgctgaa agaacgctat accacatcca
     5821 aacctgcgta taaccagata gcgattgtta acccgcagct caagccggaa cgcgctcgcg
     5881 gggtggattt aacctggaat ggtgccttca cgcgcgactg gggatttgag gtcagcgttt
     5941 actataaccg ggtgagtgat gccatcctct cgcacaatat caatgccgat accattcaaa
     6001 atcagaacag cggcacggtg gattacagcg gtctggatgc cggtattaag gggaaaatca
     6061 gcaatatact ggatgtagga ttgagctacg ctctgatcca cgctgacgcc aaacgcaaag
     6121 acatcggcaa gataaccgat cttccaacgc agacaatgac cgcatggatg acactcaaac
     6181 catgggagcc gttaagcgta acgctgtcgg aagaggcgcg ttcctccagc tacagcaaca
     6241 gtgacggttc acaaaaagcc gccggttttg cggtgaccca cattcgagcc gattacacct
     6301 taggtcatgg cttcagcgtc aatgcgtcgg tcaataacct gtttgatacc caatatgcct
     6361 acagtgaagg gtttattgaa gaaggtcgca atttctgggc aggtgtcgag tatacgttct
     6421 gatgtgaaag cggatagcgg catctgtatc gctatccgcc ttatgaaaat cttattctct
     6481 tcgcaggagg cggaatacgc ccagcaccag gatagcgccc accaccgcca ccaggaagct
     6541 gtgcaaatta aaaccgctga tggagccgtc aatgccaaac atggtcgcca gccagccgcc
     6601 gaccaccgca ccgactatcc cgagaataca ggtcaggaaa aatccaccac catcacgccc
     6661 cggcatgatt agcttgccga taatgccggc tatcaggcca aaaataatcc aggcaataat
     6721 tcccatgttc aggccctcgt ttcgatgaat cggtcgcaag aaatgcttac gtcaacgctc
     6781 taagtatagg caagggcggg atcgatgctg ggcgtttgtc ggctgattta agaacaacat
     6841 gtaaaaaata ttcacgcatt tgtattaagt tttgttaact atgaccgata aaccaaagac
     6901 agtttgtcag tcaggagttt ttccgcgtga gtcattacca tgagcagttc ctgaaacaaa
     6961 atccgttagc cgtcctgggc gtgttacgcg atttgcacaa agccgcaatt cctttgcgtc
     7021 tcagttggaa tggcgggcag ctgatcagca aaatactggc aataaccccg gataaactgg
     7081 tgctggattt cggcagtcaa gccgaagaca acatcgccgt gctaaaggca cagcacatta
     7141 ccattaccgc cgaaactcag ggtgcgaaag tcgagtttac tgttgaacaa ctacagcaga
     7201 gtgaatactt gcagcttccg gcatttatta ccgtaccgcc tcccacctta tggtttgtac
     7261 aacgacgccg atatttccgc atctccgccc cactccatcc gccttatttt tgccagacca
     7321 aactggcgga taacagtacg ttacgtttcc gcttgtatga tttgtcgtta ggcggcatgg
     7381 gcgcattact ggaaacagca aagcctgccg aattacaaga aggtatgcgc ttcgctcaaa
     7441 ttgaagtcaa catggggcaa tggggtgttt ttcactttga cgcccagtta atctccacca
     7501 gcgagcgcaa agtgattgat ggcaagaatg aaaccatcac cactccccgt ctgagcttcc
     7561 gttttcttaa cgtcagcccg acggtggagc ggcaattaca gcggattatt ttctctctcg
     7621 agcgagaagc ccgggaaaaa gcggacaaag tgcgcgactg atttacatcg cgtccagtgc
     7681 ctgctcaagt ttgtagatat agcgcggagc ctgcggcgca ggatgatttc gcgctacgtg
     7741 atcgaggaac tcgtcagcat ccaaatcgtt gattttgctg atcgcctttt tccgatctga
     7801 cgagaaagtc cgtagcagcg cacctgcccc gttagcgtat gacaccacca gcgcatattg
     7861 cagtaccttc ggatcttcaa tgcctgccag cgggccggtt tccagaatat tcaggtaagc
     7921 cgcccccatt gaaatattac gctctggatt tttcagctcg ctggtcgtcg gctcaccact
     7981 ccagcccata cggcgataaa catcacgtcc ggaggttgaa gcttttaact gcatcaaacc
     8041 aatggcattc gatttactca ccgcgttcgg attaccaccc gattcgatag cgataatcgc
     8101 cgtgatcaat tgtggatcga cgccccaggc tgcaccggct ttctggctta ttggcatcca
     8161 ctgcatcgca cgttgcaccg gaactttcgc gttccacggc gggttcgtat agtcatgctt
     8221 tgatgaacaa cccgctaata acacaatcaa aaaggcaaac catctcaatt tcacttcttc
     8281 tatccttata gcctggaatc gccgttgagg tgacaacggc acacctaagc ggcatgatat
     8341 acatttcgtt tctgttgtca gcaaggaatt gccatgtctc tgtttcactt aattgcccca
     8401 tcgggttaca gcattaaaca gcacgccgcg ctgcgtggta tccagcgcct taccgacgcg
     8461 gggcatcaag tcaataacgt cgaggttatt gcccgtcgtt gtgagcgttt tgcgggtacg
     8521 gaaacggagc gtctggaaga tctcaattcc cttgccagac tgactacccc caacaccatc
     8581 gtgctggctg tacgcggcgg ttacggtgcc agtcgtttac tggcagatat tgactggcag
     8641 gcactggtgg ctcgccaaca acatgacccg ttactcattt gcggacatag cgattttacc
     8701 gccattcagt gcggtcttct ggcgcaaggc aatgtcatca cctttagcgg ccccatgctg
     8761 gtagcaaact ttggtgcgga tgaactgaac gcctttaccg agcaccattt ctggctggcg
     8821 ttacgcaatg aaaccttcac cattgaatgg caaggggaag gcccgacatg tcagaccgaa
     8881 ggcacgttgt ggggaggcaa tcttgcgatg ctgatttcac tgattggtac accgtggatg
     8941 ccaaaaattg agaacggtat tctggtgctg gaagatatta acgtgcaccc tttccgcgtc
     9001 gaacgtatgc tgttgcagct ctatcatgcg ggaattttgc cacgtcagaa ggcgattatt
     9061 ctcggtagct ttagcggcag cacgcccaat gattatgacg caggttacaa cctggagtca
     9121 gtgtacgcgt ttttgcgttc ccgcctgtcg attccactca ttaccggcct cgattttggt
     9181 catgaacaac gcacggttac gctgccgttg ggcgcacatg ccattctgaa taacacccgg
     9241 gaaggcgctc agttgacgat tagcggtcat cctgttctta aaatgtaaaa aaatcacgct
     9301 tcagggctaa gtaaaacgct gtctctgccg ctaatatggt agggtttata taagagacag
     9361 cgtaatcagg agtaaccgac cattggatgc cacaacaata attagccttt tcatcttagg
     9421 atccatcctt gtcaccagca gtatattact tagttcattt tcttcccgtc ttggcattcc
     9481 tattctggtt atttttctgg cgatcggcat gctggcggga gtcgatggcg tcggcggtat
     9541 cccgtttgat aattacccct tcgcctacat ggtaagtaac ctggcgctgg cgattatttt
     9601 gctcgacggc gggatgcgca ctcaggccag ttcctttcgt gtggcgttag gaccggcgct
     9661 gtcgctggcg acgctgggcg tacttatcac ctctggttta accggcatga tggcggcgtg
     9721 gctgtttaat cttgatttga ttgaaggctt attaatcggc gctatcgtcg gctccaccga
     9781 tgctgcagcg gtcttttctc tgctgggtgg taaggggctt aacgaacgtg ttggctcgac
     9841 gctggaaatt gaatccggca gtaatgatcc aatggcggtc tttctgacga ttaccctgat
     9901 tgcaatgatc cagcagcatg aaagcagtgt cagttggatg ttcgtggtcg atattctgca
     9961 gcaatttggc ctcgggattg tcatcggtct tggcggtggt tatttactgc tgcaaatgat
    10021 taatcgcatc gccctgcctg ccggattata tccattgctg gcattaagcg gcggtatttt
    10081 aatttttgct ttaaccacgg cgctggaagg cagcggtatt ctggctgttt atctgtgcgg
    10141 ttttctgctg ggtaatcggc cgattcgcaa ccgctacggc atcctgcaaa atttcgacgg
    10201 cctcgcctgg ctggcgcaaa tcgccatgtt cctggtgctg gggctattgg ttaacccaag
    10261 cgatctgctg cctattgcca ttccggcgct cattttgtcg gcatggatga tattcttcgc
    10321 ccgtcccctt tcggtatttg ctggattgct ccctttccgc ggtttcaatc tgcgtgagcg
    10381 cgtgtttatc agctgggtgg gattacgcgg cgcggtgcca atcatcctag ccgtgttccc
    10441 gatgatggcg gggctggaga atgcgcgact gttctttaat gtcgccttct ttgtggttct
    10501 ggtttcactg ctcttgcagg gaacatcact ctcgtgggcg gcgaaaaaag ccaaagtggt
    10561 ggttccgccc gtcggacgtc cggtgtcacg cgttggcctg gatattcatc cggaaaatcc
    10621 gtgggagcag tttgtttatc aattgagtgc tgataaatgg tgtgtaggtg cggcactgcg
    10681 cgatttgcat atgccaaaag agacgcgtat tgcagccctg tttcgtgata accagttgct
    10741 tcatcctacc ggcagcaccc gactgcgcga aggcgatgta ttgtgtgtaa ttggtcggga
    10801 acgcgatctc ccggcgctcg gtaaactgtt cagccagtcg ccaccggtcg cgctggatca
    10861 acgcttcttt ggtgacttca ttctcgaagc cagcgccaaa tatgctgatg ttgcgctgat
    10921 atatggcctg gaagacgggc gagaatatcg tgataaacag caaacgctgg gtgaaattgt
    10981 ccagcagttg ctaggcgcgg caccggttgt cggtgaccag gtagagtttg ccgggatgat
    11041 ctggacggtg gccgagaaag aagataatga agtgttgaag attggcgttc gggtggctga
    11101 agaggaagct gaatcttaaa tttaatgcag catctatcgc cggatgcgat gctttactga
    11161 acgcagcctg agcctgtaat aaacttgtca tttcctgatt ttgatatgct catcccaaca
    11221 tcagaagaag gttaaattat ggatgacaaa ccattgcagg ctctggctaa tgaactcaaa
    11281 acaccggctc aagaaagtca gcgtaagggc tttttccgac tgtaacaggt aataaaaacc
    11341 agccccgcat taccggtcaa ccatcagggg cccctgcctg acatcgcctg aacatattcc
    11401 cccacgcaac ctgccgcact gacatagccc acacaatagg cgtttcatcc gcctgtagca
    11461 ccggatgcat gagcgatttc ttatagttat tcctttgctt aataacgatt atttccgggt
    11521 cgttcactct cgtattttct ttaatacgtc agagctacct aggccgtaat acagcatgta
    11581 ccagacccga cacggcattt ctgaccagga aaaaagtcaa cgatgagtag tgatatcagc
    11641 gcaaatgacg tcctgtggct cctttgcggt gaccacattc acgtctccgg cgctgccaga
    11701 ggatatttat ttcaaggaaa cgtagcatta tggctagctt taaagacatc tgcccattcg
    11761 cccccgccat tcccccatct tccgttaacc caggcgtacg acgcccgcca gggcggctct
    11821 tctcaagaat caaattttgc agaacatgaa gtatctcagc ccgcaaaaat tcagctgggg
    11881 tgatgccccc tggcagatca ttgacctcag tatcgcgggc aaagtgaaca tacaggtgga
    11941 caacaatacc atcatcacgt tggggactcg tctgaatcag caacataacg agttcatgat
    12001 ggtcgccaaa tggtgcgaat gggctataca gcaggatggt ttgcaagaaa acctgcaaaa
    12061 aaacctgtac gaaatcctgg aagaaaacca gcaaaacaaa cagtcagaaa ttccccaaga
    12121 agacctgaaa gaaagtctgg aagaaatcaa ggaaaatatc ctggaagaaa acctgccagc
    12181 aagccggatt gaaaaccggg cagaagcatt gcgcagaatg aaggaatgtc tcattacaag
    12241 acggagtatg ctcaatctta gcaacctggg attgacttca ctccctgaga atttgcctcc
    12301 acatctgatt gaattttact gcagtaaaaa cgtgttgacc gcgttaccga aggtgatgcc
    12361 aaagtggctg ctggtgcttg actgtacaga taacgtcttg atcttgttac cgaaggtgca
    12421 gccctcaaaa ctgatggtac tgaagtgcta tgaaaacagt attatctggc tgcctgagct
    12481 gtcgactaac ctgagagtga ttaactgttc tgaaaacttc ttgcaatttt taccgccgtc
    12541 gatgccccag tacctgtata aactatcctg tgccggcaac aatattaact caatacctga
    12601 tgagatgctg gagaacttga ctcgcctgaa agtatttgac tgttctagta acgatttgat
    12661 ctcttcacca cggctgccgc ccaaactaat aatttactac tgtggagaaa acaagtttaa
    12721 aacggtacag gtgccgcagc cccagagcct gaaggtgttt gactgtaatg gtaacccgtg
    12781 ggacaaagac aatttaccga cgctgctcaa agccgtcgag ggcctgaaaa aacagcaggg
    12841 cctgaaggac cttttggact ttttgcacaa ggaaggctag gttgacctgg aaggactcga
    12901 ggagctggag gaccttatgg atctggagtt cctggatgac ccggaactcc tggagcgcgt
    12961 gaaggtacag gaggacctgg agctcctgga tcaacagttg ggcctgttga gtctggaaag
    13021 acaacaggac tcgcagcctg ttaatcaaca atctgaacat gaacccgaat ctgcatcaag
    13081 ggtgaagcgt gatttatctg aagtcgactc cgagtcaaca atgaagcgta agcgttttat
    13141 gtagaacgcc tgtgcggcga gttcaggttc cggggaaata gtgtccattc ttttaatgga
    13201 cactatcatg ggcaatggct ctgtcacatt aacaactaca gttgctgacc agccggatgg
    13261 atgatttttt cgcaaccaga agacgctgcc tccgatccgg cttacaacaa gttacactgt
    13321 cacaaccggg acgcgtaacg ccagtgcgca catcaactca tagcccaccg ttccggcagc
    13381 ggcggcgaca tcatcaattt tgatctcctt gccccacagc tcaaccggcg taccaatccc
    13441 cgcctgcggg caaggcgtta aatcaaccgc cagcatatcc atcgagacgg tccccaccgt
    13501 catggtgcgc acgccgtcca ctaaaacagg agcaccggta ggcgcatggc gcggataacc
    13561 gtcggcgtat cctgcggcga caatgccgat tcgctgttca tcgcgcgcag tatagcgacc
    13621 gccgtagccc acacgctcgc ccgcttttag cgtctggaca ccaataatct cactgcttag
    13681 cgtcatcacc ggacgtaatc cggtattggc gatatcacgc cactgaccgg acggcgaagc
    13741 gccatacaaa ataatgccag gccgaaccca gtcaaaatgc gcttccgggt gccacagagt
    13801 cgccgccgaa ttggacaacg aacgccgaca ctccagcccc tccgccgcct gctcaatacg
    13861 cgccatcgcg ccggaaattc catcaggatg ttccgcttca gcaaaatgcg acatcagggt
    13921 catttcgcca acattcgcca ttgcccgcaa ctgctgccag acggtaagca cgcgatcgga
    13981 ctggaagccc aaccgattca tcccactgtt cactttaaga taaatatcca acggtgcttt
    14041 tagccgcgca ttttgcagtg ctttgagctg ccagttgctg tgtacgcagg tggtcaggcg
    14101 gtgctggtca taaatctcca gatcctgagc atggaaaaat ccttccagca tcaggatcgg
    14161 ccccttccag ccgcgctcac gtaacgttat tgcctcttcc aggttaagta atgcaaagcc
    14221 atcggtggcc ccgagcgcgc tccagatacg ctcaatacca tgcccgtaag cgttcgcttt
    14281 taccaccgac cagacgcgcg cgtgcggcgc ggcctggcgg acaatggaca gattctgttt
    14341 taatgcctgc agatcgaggc tggcctgtat cggacgggtc atctcgtttc cttagctgtg
    14401 tgcgccatgt aaatggcccg gacgtgatgg cgtaaatcca cggctgtagc gcgctacgct
    14461 tagatcctca tatgggatcg ctggcgtgcg accagagagc agatcgctta acaactgacc
    14521 ggaaccgcaa gccatcgtcc agccgagcgt gccgtgaccg gtattcagcc acagattttt
    14581 aaagcgtgta ctcccgacaa ccggcgtacc gtctggcgtc atcgggcgca aacccgtcca
    14641 gaaagtcgcc tgctcgacat gaccgccgcg tggatagaga tcgcgaacca ccatttccag
    14701 cgtttcacga cgcggttgca acagctcggt gttaaaacca acaatttccg ccattccgcc
    14761 aacacgaatg cggttatcga aacgggtaat ggcgattttg taggtttcat caagaatggt
    14821 ggatactggc gcaccatctt cctgcgcaat tggaatagtc agcgagtagc ctttcagcgg
    14881 atacaccgga atatcaacaa tgcctttgag catcgccgtc gagtaagaac caaacgccat
    14941 cacatacgca tcggccttaa tcacttcatc gccaaacttc acgccgtaga tttgctcgcc
    15001 gtcgcaaagc agttggtcaa cgggcgtatt aaagcggaat ttaacccccg cctgctctgc
    15061 catccgcgcc agattctggg taaacagctg gcagtctccg gtttcatcat tgggtaactg
    15121 caggccgccc gtcagtttgt gcgctacttc tgccagcgcg ggatccactt ccgccaggcg
    15181 gctggattcc agcagctgat acggtacgcc ggcatcttcc agcacggcga tatcgcgggt
    15241 cgcattttca tactgttgtt cggtacggaa cagttgcagc gtcccaccct gacgcccttc
    15301 atactgaata ttggtttcgg cgcgcaatgc tttcaggcaa tcacggctgt attccgccag
    15361 acgcaccatc cgccctttgt tttccatgta gtggctggtg tcgcagttac gtaacatttg
    15421 ccacatccat ttcaactgaa actgcgtacc gtcgagacga accgccagcg gcgcatggcg
    15481 ctggaacatc catttaatcg cttttaaagg cacacctggt gctgcccacg gtgcagcata
    15541 tccgggggag atttgcccgg cattcgcggc actggtttcc agggcggccc ccggctcccg
    15601 atcaatgacg gtgacctcat gtcctgcctg atttaagtac caggcgctgg caacgcctac
    15661 cacaccactt cccagtatga caactcgcat agccactccg ataacggtag atacagtaaa
    15721 agaataataa tctaattaca tcttgataac tcagttgaaa atattattca acatacggct
    15781 tttttatggt gagctgactc acatctccct gactctgcgg caatatggcg tctttagtat
    15841 ctcctgctga acgccctttt tatccagcat aaaattccgt tcagaagcgg attagtggca
    15901 ctctgataat aagaaatcgc aaagaaaaaa attctccggc gacgcggttt ttaacaggtg
    15961 ttctatgctt gaaatgaggt gctccacaca gagagcgccg acaacaatga ggtgcgcgta
    16021 tggcgacgat cgattctatg aataaggaca ccacacgttt gagcgatgga cccgactgga
    16081 cgttcgacct gctggatgtt tatctggcag agatagaccg ggtggcgaaa ctctaccggc
    16141 tggataccta cccgcaccag attgaagtga taacctcaga acagatgatg gatgcctatt
    16201 ccagcgtcgg catgccaatt aactatccgc actggtcatt cggtaaaaag tttatcgaga
    16261 ctgaacggct gtataagcac ggtcagcaag ggctggccta tgaaatcgtc attaactcta
    16321 acccgtgtat cgcttacttg atggaagaga acaccattac tatgcaggcg ctggtgatgg
    16381 cacacgcctg ctatggacat aactctttct ttaaaaacaa ttgcttattc cgcagttgga
    16441 ccgacgccag ttcgattgtc gattatctga tttttgcccg taaatatatt accgagtgcg
    16501 aagaacgtta tggcgttgat gaagtagaac ggcttctgga ctcgtgccac gcgctgatga
    16561 actacggcgt ggaccggtac aaacgcccgc aaaaaatctc gctgcaagaa gagaaagccc
    16621 ggcagaaaag tcgcgaagag tatctacaaa gtcaggtcaa tatgctctgg cgtaccctgc
    16681 cgaagcgcga ggaagagaaa acggttgctg aagcgcgccg ctatccgtcc gaaccacaag
    16741 aaaacctgct ctattttatg gagaaaaatg cgccactgct ggaatcatgg cagcgtgaaa
    16801 tcctgcgtat tgtgcgtaag gtgagccagt atttttatcc gcaaaaacag actcaggtga
    16861 tgaacgaagg ctgtgcgacc ttctggcact ataccatcct taaccatctg tatgatgaag
    16921 ggaaagtaac ggaacgtttt atgctggagt ttttgcacag ccacaccaat gtggtcttcc
    16981 agccccccct ataacagccc gtggtacagc ggcatcaacc cgtatgccct cgggttcgcc
    17041 atgttccagg atattaaacg gatttgtcag tcgccaacgg aagaagacaa atactggttc
    17101 ccggatatcg ccggttccga ctggctggaa acgctgcatt tcgcgatgcg tgatttcaaa
    17161 gatgagagtt ttatcagcca gtttctgtca ccgaaagtga tgcgtgattt ccgcttcttc
    17221 accgtgctgg atgatgatcg gcataattat ctggagattt ccgctattca taatgaagaa
    17281 ggttatcggg agatccgtaa ccggttatcg tcgcaatata acttaagtaa tctggagccg
    17341 aatattcaga tctggaacgt ggatttgcgc ggcgaccgtt cgctgacgct gcgttatatt
    17401 ccacataatc gcgcaccgct ggatcggggg cgcaaagaag tcctgaagca tgtgcatcgc
    17461 ctgtggggat ttgatgtgat gctcgaacag caaaacgaag acggcagcat cgagttgctg
    17521 gaacgttgcc cgccaagaat gggaaatctg taaaaacaac aaaaaacccc tcgtttgagg
    17581 gggttgctct ttaaacggaa gggattatcg cccctgaatg gctaaatcac ccggcagatt
    17641 tttctgcatc cggtgccaaa tctcgccact ctcatgccca tagcgacgca ctgtttcgta
    17701 cacctgatcg tgcgcgcctt cactgcacaa cgccgacagt ttgtggtaga agcccagcgc
    17761 cagactgcgc gcttccggat tggcgaaata gtgacgacca atgcgcgtat acagcccttt
    17821 catcccgtta aggatcagac cgtaaatcgg gttgccggaa gcaaacgcca ggccgcggaa
    17881 tatgttgtaa tccagctcgg caaaggcatc ggcgtgatcg gccacttcat tagcggtagc
    17941 cagcacttcc tgcgctttat cgggatgctg acgaaacgcg gtgcgaataa aaatagtgga
    18001 aatattggta cgcaccgaca gcaaattatc aataagctgc ggcacacttt cgtgatccag
    18061 tcgcgccagt gtttcaagga tatttaaacc ggaagtttcc cagaaattat tcaccttcgt
    18121 cggcttgcca tgttgaatgg tcaaccagcc atctcgtgcc agacgctgta acacttcacg
    18181 taacgtagta cgcgttacgc caattaattc tgaaagttca cgttctgcgg gcaaaatagt
    18241 cccgggaggg aagcggttat tccagatact ttcaataatg tactcttccg cgaaacccgc
    18301 cgggctttgc gccttaatga ccatagtgag atttccataa cacagcaaaa caaagttgga
    18361 ctcatcatac cagagggcta actacgctga tagcagacaa aaagaagaaa aagggatcaa
    18421 ggcttcattt tccgcatctc tttccccttg actatcaaga cttgcctcac tcctgcctga
    18481 ccgttaagct ttgcgtttaa acataaaaac atcattgatt atttgtaagg taagggaaac
    18541 catcatggag atctcctggg gccgcgcgct atggcgcaat tttttgggcc agtcccccga
    18601 ctggtacaaa ctcgccctca tcattttctt aatcgtaaac ccgttaattt tcctcatcag
    18661 ccctttcgtc gcgggctggt tgctggtagc ggaatttatt ttcactctgg cgatggccct
    18721 gaaatgctac ccgctactcc ccggtggtct gttggctatc gaagcggtat tcatcggcat
    18781 gaccagcgcg gaacacgtcc gtgaagaggt ggcggcaaat cttgaagtct tgctgttatt
    18841 gatgtttatg gtggcgggta tctattttat gaaacagctg ttgctgttca tatttacccg
    18901 tttgctgtta agcattcgct ccaaaatgct gctgtcgctc tctttttgcg tggcggcagc
    18961 gttcctctcc gcgttcctcg atgccttaac cgtcgtggcg gtggtgatca gcgttgcagt
    19021 cggtttttat ggtatttatc atcgcgtagc ctcttcccgt accgaagaca ccgacctgca
    19081 agacgatagt catatcgaca agcattacaa cgtggttctg gaacagttcc gtggctttct
    19141 gcgtagcctg atgatgcatg ccggtgtcgg caccgcatta ggcggcgtaa tgaccatggt
    19201 gggcgaacca cagaacctga tcatcgctaa agcggctggc tggcattttg gcgatttctt
    19261 cctgcgcatg tcgccggtga ccgttccggt tctgatttgt ggcctgttaa cctgcctgct
    19321 ggtagagaag ctgcgttggt ttggctacgg tgaaacgctg ccggagaaag tccgcgaagt
    19381 gttgcaacag tttgacgatc aaagccgcca ccagcgtacc cgtcaggata aaatccgtct
    19441 gattgtccag gcgattattg gcgtctggct ggtgactgcg ctggcgttgc atctggcgga
    19501 agttggcttg attggtttgt cagtcattat tctggcaaca tcattgaccg gtgtcaccga
    19561 tgaacatgct atcggtaaag ccttcaccga atctctgcca ttcaccgcac tgttgacggt
    19621 gtttttctcg gtagtcgcgg tgattatcga ccaacaactg ttttcgccga ttattcagtt
    19681 tgtgttgcag gcatctgaac atgctcagct gtcgctgttc tatattttca acggtctgct
    19741 gtcatccatt tcggataacg tcttcgtggg gacgatttat atcaacgaag cgaaagcggc
    19801 aatggaaagt ggcgctatca cgttgaagca atacgagctg ctggcggtcg ccattaatac
    19861 cggtaccaat ctgccctctg tcgctacgcc gaacggtcag gctgcgttcc tgttcctgct
    19921 gacctctgca ctcgcgccat tgattcgcct ctcttatggc cgcatggtgt ggatggccct
    19981 gccttacacc ctcgtactga cactcgtcgg cttgctctgc gtcgagttta cgcttgcccc
    20041 tgtaaccgaa tggtttatgc aaatgggctg gatagcaacg ctttgataac aacttaccgg
    20101 gcatataaat gcccggtttg ccttttcgcc cgataattgt ccaattgcgt cctatttttg
    20161 ctgcctcctg gtggcgcagc gaatgaattg gtttaaactg cgcactctat gcatattgca
    20221 gggaaatgat tatgttgcga tttttgaacc aatgttcaca aggccggggc gcgtggctgt
    20281 tgatggcgtt tactgctctg gcactggaac tgacggcgct gtggttccag catgtgatgt
    20341 tactgaaacc ttgcgtgctc tgtatttatg aacgctgcgc gttattcggc gttctgggtg
    20401 ctgcgctgat tggcgcgatc gccccgaaaa ctccgctgcg ttatgtagcg atggttatct
    20461 ggttgtatag tgcgttccgc ggtgtgcagt taacttacga gcacaccatg cttcagctct
    20521 atccttcgcc atttgccacc tgtgatttta tggttcgttt cccggaatgg ctgccgctgg
    20581 ataaatgggt gccgcaagtg tttgtcgcct ctggcgattg cgccgagcgt cagtgggatt
    20641 ttttaggtat ggaaatgccg cagtggctgc tcggtatttt tatcgcttac ctgattgtcg
    20701 cggtgctggt ggtgatttcc cagccgttta aagcgaaaaa acgtgatctg ttcggtcgct
    20761 aatccattcg gcgctcctgc gggagcgctt ttttcctgcc gctatattta tttgacccgc
    20821 agtaaatcag aacttcgcgt tgtataacgt ggtgaaagca tagctcgctt catctgccat
    20881 tgttgttgga tcccctgccc ggcaaaatag agtgttcctc tgccctcttt agcattcaga
    20941 gtatccatta ccgccatcaa ttgctcactc ccggggcgcg gtgcgttgtc atcgaataaa
    21001 ttgagctgcg cgactccctg actgaagaaa tcccccagca tcacgcccgc tttttggtag
    21061 cgatggcccg cttgccagat ggcatccaga gatcgcgtag cagcgttaat gatatccctg
    21121 ctgtcctgag tgggcgtcag cagttttacc gacgcgctat tgccgtaata aggttcattg
    21181 agcgcaaatg gtgacgtctt aataaatgtg gagataaacc gacaatattg atgctcactg
    21241 cgaagttttt ccgccgcccg ggcagcgtaa ctacaaatgg cctgccgcat cgacgtataa
    21301 tccgtgatgc gttcaccaaa cgagcgggaa cagataattt cctgcttcgt cggtgcaaac
    21361 tcttccagtt gcaaacaggg ttcgccgcgc agttcacgca ccgttctttc gagcatgaca
    21421 ttaaaatgtt tacggataaa ccggatatct gtatccgcca aatcgagaac ggttttgatc
    21481 cccatcgcgt ccagtttttt gctgatccgc cgtccaatcc cccagacgtc atccacgggg
    21541 agagcagaca ttaatttacg ctggcgttcc agatttgata aatccaccac cccacccgtc
    21601 tgccgctgcc atttttttgc cgcatgattg gcaagcttag ccaacgtttt ggtctgggcg
    21661 atccccacac caacagtaag atgggtacgt tgtagcaccg ttgcgcgaat ttctctgcca
    21721 aaatcagtca gatcgcgaca attacgcaca cctgtcagat cgcagaatgc ttcatcaata
    21781 ctgtaaatct cgacgcgggg cgatagttct tccagcgtcg acatcacccg attgctcatg
    21841 tctgcgtaaa gctcataatt gctgctaaag caaaccacac cacagcgacg aaacagatct
    21901 ttttgtttga accagggatc gcccatttta acgcccagcg cctttgcctc agcgtttcgg
    21961 gcgataacgc aaccgtcatt attcgatagc acaaccaccg gtttacccca taaatcaggg
    22021 cgaaacaccg tctcacagct ggcataaaac gcgtttacat cacagagggc aaacatcagc
    22081 gcatcgcctt aacgacgtgg atcaccacac caaagacatc cagcgtatct tcgctactga
    22141 tggtaatggg cgagtacgca ctgttcatgg gaataagctg taccgtcggg cgtagttgca
    22201 attttttcac cgtaaactcg ccgtcaacag cagcgatgac aatatcacca tggctggcgg
    22261 taatagcact atcgacaatc agtaaatcac cgtcactaat tccaccatca atcatagaat
    22321 caccacttgc tttgacgaag taagtcgcgc tgggatgctg gatcaacagt tgattcagat
    22381 cgatgcgctg ttcaacgtaa tctgctgccg gtgaaggaaa gccacactga acaagatcgc
    22441 taaatagcgg aaaagtcaca atttcgcgga gatccgcagg cttgataaac aacataataa
    22501 tctgcctgaa gttatactgt ttttatatac agtggtctgt tcttgccagc agatcaatac
    22561 tgattcaggc tatcaatatt tgtcgctgca tagactgctg atttttcgtt ctcttatcct
    22621 gtgctcacgt ggccttctgg cgacgacgct catccagcag aaatgaaaaa tatcacccgg
    22681 caaaaaaata g
//
LOCUS       DAZSNO010000051        22412 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZSNO010000051 DAZSNO010000000
VERSION     DAZSNO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.051"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            82..2682
                     /locus_tag="ACHWN2_001938"
     CDS             82..2682
                     /locus_tag="ACHWN2_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFR8931493.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNRGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3742
                     /locus_tag="ACHWN2_001939"
                     /pseudo
     CDS             2673..3742
                     /locus_tag="ACHWN2_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3754..4294
                     /locus_tag="ACHWN2_001940"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="ACHWN2_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="ACHWN2_001941"
     CDS             4302..4817
                     /locus_tag="ACHWN2_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8931494.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="ACHWN2_001942"
     CDS             4783..5520
                     /locus_tag="ACHWN2_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR8931495.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHWN2_001943"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHWN2_001943"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR8931496.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="ACHWN2_001944"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="ACHWN2_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR8931497.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHWN2_001945"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHWN2_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR8931498.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHWN2_001946"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHWN2_001946"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR8931499.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="ACHWN2_001947"
     CDS             10827..12734
                     /locus_tag="ACHWN2_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8931500.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHWN2_001948"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHWN2_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR8931501.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHWN2_001949"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHWN2_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR8931502.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHWN2_001950"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHWN2_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR8931503.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="ACHWN2_001951"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="ACHWN2_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR8931504.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHWN2_001952"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHWN2_001952"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR8931505.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="ACHWN2_001953"
     CDS             complement(17402..19162)
                     /locus_tag="ACHWN2_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8931506.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="ACHWN2_001954"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="ACHWN2_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR8931507.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHWN2_001955"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHWN2_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR8931508.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHWN2_001956"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHWN2_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR8931509.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22412
                     /gene="sxy"
                     /locus_tag="ACHWN2_001957"
     CDS             22005..>22412
                     /gene="sxy"
                     /locus_tag="ACHWN2_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR8931510.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5721 a         5257 c         5874 g         5560 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accgtggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 cgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgtac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ctaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tg
//
LOCUS       DAZSNO010000052        22264 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZSNO010000052 DAZSNO010000000
VERSION     DAZSNO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22264
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.052"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            240..866
                     /locus_tag="ACHWN2_001958"
     CDS             240..866
                     /locus_tag="ACHWN2_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR8931511.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            887..2988
                     /locus_tag="ACHWN2_001959"
                     /pseudo
     CDS             887..2988
                     /locus_tag="ACHWN2_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3001..3639
                     /locus_tag="ACHWN2_001960"
     CDS             3001..3639
                     /locus_tag="ACHWN2_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR8931512.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3703..4371
                     /locus_tag="ACHWN2_001961"
     CDS             3703..4371
                     /locus_tag="ACHWN2_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR8931513.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4368..5153
                     /gene="phsC"
                     /locus_tag="ACHWN2_001962"
     CDS             4368..5153
                     /gene="phsC"
                     /locus_tag="ACHWN2_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR8931514.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5157..5969
                     /gene="ydhT"
                     /locus_tag="ACHWN2_001963"
     CDS             5157..5969
                     /gene="ydhT"
                     /locus_tag="ACHWN2_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR8931515.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5981..7403)
                     /locus_tag="ACHWN2_001964"
                     /pseudo
     CDS             complement(5981..7403)
                     /locus_tag="ACHWN2_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7529..7834)
                     /locus_tag="ACHWN2_001965"
     CDS             complement(7529..7834)
                     /locus_tag="ACHWN2_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR8931516.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(7942..8018)
                     /locus_tag="ACHWN2_001966"
     tRNA            complement(7942..8018)
                     /locus_tag="ACHWN2_001966"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7982..7984),aa:Val,seq:gac)
     gene            complement(8023..8099)
                     /locus_tag="ACHWN2_001967"
     tRNA            complement(8023..8099)
                     /locus_tag="ACHWN2_001967"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8063..8065),aa:Val,seq:gac)
     gene            8407..9663
                     /locus_tag="ACHWN2_001968"
     CDS             8407..9663
                     /locus_tag="ACHWN2_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR8931517.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9704..11077)
                     /gene="mdtK"
                     /locus_tag="ACHWN2_001969"
     CDS             complement(9704..11077)
                     /gene="mdtK"
                     /locus_tag="ACHWN2_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR8931518.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11292..11933
                     /gene="ribE"
                     /locus_tag="ACHWN2_001970"
     CDS             11292..11933
                     /gene="ribE"
                     /locus_tag="ACHWN2_001970"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR8931519.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(11973..13121)
                     /gene="cfa"
                     /locus_tag="ACHWN2_001971"
     CDS             complement(11973..13121)
                     /gene="cfa"
                     /locus_tag="ACHWN2_001971"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR8931520.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13412..14623)
                     /gene="punC"
                     /locus_tag="ACHWN2_001972"
     CDS             complement(13412..14623)
                     /gene="punC"
                     /locus_tag="ACHWN2_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR8931521.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            14736..15668
                     /gene="punR"
                     /locus_tag="ACHWN2_001973"
     CDS             14736..15668
                     /gene="punR"
                     /locus_tag="ACHWN2_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR8931522.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(15665..16690)
                     /gene="purR"
                     /locus_tag="ACHWN2_001974"
     CDS             complement(15665..16690)
                     /gene="purR"
                     /locus_tag="ACHWN2_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR8931523.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            16989..17078
                     /gene="cydH"
                     /locus_tag="ACHWN2_001975"
                     /gene_synonym="ynhF"
     CDS             16989..17078
                     /gene="cydH"
                     /locus_tag="ACHWN2_001975"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR8931524.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            17244..18413
                     /locus_tag="ACHWN2_001976"
     CDS             17244..18413
                     /locus_tag="ACHWN2_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8931525.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18559..19140)
                     /gene="sodB"
                     /locus_tag="ACHWN2_001977"
     CDS             complement(18559..19140)
                     /gene="sodB"
                     /locus_tag="ACHWN2_001977"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR8931526.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19268..20095)
                     /gene="mepH"
                     /locus_tag="ACHWN2_001978"
     CDS             complement(19268..20095)
                     /gene="mepH"
                     /locus_tag="ACHWN2_001978"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR8931527.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20430..20777
                     /gene="grxD"
                     /locus_tag="ACHWN2_001979"
     CDS             20430..20777
                     /gene="grxD"
                     /locus_tag="ACHWN2_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR8931528.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20891..21301)
                     /gene="tnpA"
                     /locus_tag="ACHWN2_001980"
                     /pseudo
     CDS             complement(20891..21301)
                     /gene="tnpA"
                     /locus_tag="ACHWN2_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            21324..>22264
                     /locus_tag="ACHWN2_001981"
     CDS             21324..>22264
                     /locus_tag="ACHWN2_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR8931529.1"
                     /translation="MKRLQAFKFQLRLGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEY"
BASE COUNT         5758 a         5213 c         5778 g         5515 t
ORIGIN      
        1 taatttcttg ctttgtaatt ttaattgata acgatcaatg ttaagggctc agaataatca
       61 cattattttt tctcctaacc ataaaggatt agtttatcgg cgttaaatct tttctcatac
      121 actatcgggt tagggaaaat atctggtgtt ggggacagag tcagcagcgt attagttgaa
      181 tgcttaatat caggctgctg aattgtcagc actgtgtttg cgaagaggat gcgaaataaa
      241 tgaacccggt tgatcgtcca ctattagata ttggcttaac gcgattagaa ttcttgcgta
      301 tatcaggaaa aggccttgct ggtctaacaa ttgcccctgc gttactgtca cttttgggtt
      361 gcaaacagga agatattgat agcggcacag tagggttgat aaatacaccc aaaggggtgc
      421 tggtgaccca gcgagcgcga tgcacgggtt gccaccgctg tgaaatctct tgtaccaact
      481 tcaacgatgg ctcagtaggg acattcttct cccgtatcaa aatccatcgc aattatttct
      541 ttggcgacaa cggggttggc tctggcggcg gcctgtatgg cgatctcaac tataccgcgg
      601 acacctgccg tcaatgcaaa gagccgcaat gcatgaacgt ctgcccgatt ggtgcgatta
      661 cctggcagca gaaagaaggc tgtattaccg tcgatcataa acgttgtatt ggctgtagcg
      721 cctgtactac ggcatgcccg tggatgatgg ccaccgtaaa taccgaaagt aaaaaatcct
      781 cgaaatgtgt gttatgcggt gaatgcgcaa acgcctgccc aacaggggcg ttaaaaatta
      841 tcgagtggaa agatattact gtgtgaatct tgcaaaggaa aaaatcatgg ctaacggttg
      901 gacaggtaat atattaagag tcaatctcac gacaggaaat attaccctcg aagattccag
      961 taagtttaaa agttttgtcg gtggcatggg cttcggctac aaaattatgt atgacgaagt
     1021 accgctaggc acgaaacctt tcgatgaagc gaataaatta gtctttgcta ccggcccatt
     1081 acctggatct ggtgccccct gtagttctcg cgtaaatatc acctcacttt ctacttttac
     1141 caaaggaaat ttagtcgcga tgcccatatg ggtggctttt ttgcagcgca aatgaaattc
     1201 gctggatacg acgtcattat tatcgaaggg aaggcgaaat caccggtatg gctgaagatt
     1261 aaagatgaca aagttagcct ggaaaaagcc gatttcttat ggggaaaagg gacgcgcgca
     1321 acgacggaag aaatttgtcg attgaccagc ccggaaacct gtgtggcggc tattggtcag
     1381 gctggggaaa accttattcc tctctctggc atgttgaata gccgtaacca cagcggcggt
     1441 gcgggaactg gcgcaataat gggttcgaaa aacctgaaag cgattgcggt tgaagggacg
     1501 aaaggggtca acattgccga tcgtcaggag atgaagcgtc tcaatgatta catgatgact
     1561 gaacttattg gtgcgaataa caaccatgtc gtgccaagta cgccacaatc gtgggcagag
     1621 tattcagatc ccaagtcacg ctggacagca cgtaaagggc tgttttgggg cgcggctgaa
     1681 ggtggtccga ttgaaacggg tgaaattccg ccaggcaatc agaatacggt cggctttcgt
     1741 acctataaat ccgtttttga cttaggaccg gcggcagaga aatacacagt aaaaatgagc
     1801 ggctgccact cttgcccgat ccgttgtatg acccaaatga atattcctcg ggtgaaagag
     1861 tttggcgtgc ccagcacagg tggtaacact tgtgtagcaa actttgtcca taccaccatc
     1921 ttcccgaacg ggccgaaaga ttttgaagat aaagacgatg gtcgtgtgat tggtaacctg
     1981 gttggtctga atttgttcga tgactacggc ctatggtgta actacgggca gttgcatcgc
     2041 gactttactt attgttacag caaaggtgtg ttcaagcgtg ttctgccagc tgaagagtat
     2101 gcagaaattc gctgggatca actggaagcg ggtgacgtta acttcattaa agatttttac
     2161 taccgtctgg cgcatcgtgt gggtgagctg agtcacctgg ctgatggttc atatgccatc
     2221 gcagaacgct ggaatttggg taaagagtac tggggctacg cgaaaaataa actctggtcg
     2281 ccgtttggct atccggttca ccatgccaat gaagcgtcag cgcaggtcgg ttccattgtt
     2341 aagtgtatgt tcaaccgtga ctgcatgacg catacccata tcaactttat tggttccggc
     2401 ttgccattga aactgcaacg tgaagtggcg aaagaacttt ttggttctga agatgcttac
     2461 gatgaaacca aaaactacac gccaatcaat gacgcaaaaa ttaaatatgc aaagtggtcg
     2521 ctgttgcggg tctgtttgca taacgccgtc actctgtgca actgggtctg gccaatgacc
     2581 gtttcgccgc tgaaaagccg taattaccgg ggcgatctgg cgcttgaagc caaattcttc
     2641 aaagcgatca ccggcgaaga gatgactcag gaaaaattag atttagctgc agagcgtatc
     2701 tttacgttgc atcgtgccta cacggtaaaa ccgatgcaaa ccaaagatat gcgtaatgaa
     2761 cacgatctta tctgttcctg ggttttcgac aaggatccgc agatcccggt ctttactgaa
     2821 ggtactgaca aaatggatcg tgacgatatg catgcttcgc tcacgatgtt ctacaaagaa
     2881 atgggctggg acccacagct tggttgtcca acccgcgaaa cattgcagcg tctggggctg
     2941 gaagatattg ccgccgatct ggcagcacac aatctactgc ctgcgtaagg aatgggtaag
     3001 atgaaccatc aggatgaatt gccgttagcg aaagtcagcg aagttgacga ggcgaagcgg
     3061 caatggttgc aggggatgcg tcaccccgtt gatacggtaa ccgaaccaga acctgctgaa
     3121 atcctggctg agttcattcg ccagcattct gcggctgggc agctggtggc gcgcaccgtg
     3181 tttctctcgc caccctattc ggttgcagaa gaggaactgt cagttttgct ggaaagcatt
     3241 aagcagaacg gagactacgc ggatatcgct tgtatgacgg gaagtcagga tgattactat
     3301 tactcaacgc aggccatgag tgaaaactac gcggcgatgt ctttgcaagt tgtggagcag
     3361 gacatttgtc gcgccattgc acacgccgtt cgttttgagt gccagacgta tcctcgtcct
     3421 tacaaagtcg ccatgctgat gcaagcgcca tattacttcc aggaagcgca gattgaagcc
     3481 gcgatcgccg cgatggatgt tgcacctgaa tatgccgata tacggcaggt ggagtcatcg
     3541 acggcggtgt tgtacttgtt tagcgagcga tttatgacct atggcaaggc ttatggctta
     3601 tgcgaatggt tcgaggtaga gcagtttcag aacccctgaa atttcaggga gatgtcatgg
     3661 atagggtgga cagttgcagc cactgcatta ggggataatc agatgtcctt cactcgacgc
     3721 aaatttgttc tggggatggg aacagtaatc ttttttactg gttctgcgtc ttctctgtta
     3781 gcgaacacga ggcaagaaaa ggaagttcgg tatgccatga ttcatgacga gtcacggtgc
     3841 aatggctgta atatttgcgc ccgcgcatgc cgtaaaacta accacgtccc cgctcaggga
     3901 agccgtttat cgatagcaca cattccagtc accgataacg acaacgaaac gcagtatcac
     3961 ttttttcgcc agtcatgtca gcactgcgaa gatgctccgt gcatcgacgt atgcccgacg
     4021 ggggcatcgt ggcgtgatga gcaggggatc gtgcgggtag aaaaatcgca gtgcatcggc
     4081 tgcagttatt gcattggcgc ttgtccgtac ctggttcgct accttaaccc tgtcactaaa
     4141 gtggcagata aatgtgattt ctgcgctgag tcccgactgg caaaaggctt tccgcccatt
     4201 tgcgtcagcg cctgtccgga acacgcattg atctttggtc gcgaagatag cccagaaatt
     4261 caggcgtggt tgcaggataa taaatactat caatatcagc tacctggtgc cggaaaaccg
     4321 catctgtatc gccggttcgg tcaacatttg attaaaaagg aaaatgtatg aacccgtcgc
     4381 aacatgctga acagtttcag agccagttag cgaactatgt gccacagttc actcccgaat
     4441 tttggccggt gtggttgatc attgccggag tattgctggt tgggatgtgg ttggtgctgg
     4501 ggctgcacgc cttgcttcgt gctcgtggcg tgaagaaatc agccaccgat catggtgaga
     4561 aggtttatct ttacagcaaa gcggtcagat tatggcactg gtcgaatgcg ttactctttg
     4621 tattgttgct ggcaagtggg ctgataaatc actttgcgat ggtgggcgca actgcggtta
     4681 aaagtctggt tgcggtgcat gaagtttgcg gatttttgtt actggcatgc tggctcggct
     4741 ttgtgctgat caatgccgtt ggggataatg gtcaccacta tcgcattcgt cgtcaggggt
     4801 ggctggaacg agcggcaaaa caaacgcgat tttatttgtt tggcattatg cagggggaag
     4861 aacatccttt cccggcaaca acacagtcta aatttaatcc cttacagcag gtcgcctatg
     4921 ttggtgtcat gtatggattg ctgccgttgt tactattgac ggggctgttg tgtctctatc
     4981 cgcaagccgt gggagatgtg tttcctggcg taagatactg gttattgcag gcgcattttg
     5041 ctctggcatt tataagcctc ttttttatct tcggtcatct ttatctttgc accacggggc
     5101 gtacgccaca cgaaaccttt aaaagcatgg tcgatggcta tcaccggcac taacgaatga
     5161 tcattacacg agcagacctg cgagaatggc gtatcggagc ggtgatgtat cgctggttcc
     5221 tgcgccattt cccacgcggc ggtagttatg ccgatattcg tcatgcactg attgaggaag
     5281 gatatacgga ctgggcggag agtctggttg agtatgcgtg gaaaaaatgg ctggcggatg
     5341 aaaacttcgc gcatcaggaa gtttcctcta tgcaaaaact ggccacagac cccggagaga
     5401 taccattttg cagtcagttt gcccgcagcg acgatcatgc tcgcatcggc tgttgtgagg
     5461 ataacgcccg aatcgcaacc gctgggtatg ccgcgcaaat tgccagtatg ggttatagcg
     5521 tgcggattgg aagtgtcggc tttaacagtc acattggcag ctccggtgaa cgcgcacggg
     5581 ttgctgtaac cggtaattct tcgcggataa gcagcgctgg agatagtagt cggattgcga
     5641 ataccggaat gcgagtacgc gtttgtacat tgggtgaacg gtgtcatgtc gccagtaatg
     5701 gtgacctggt gcaaatcgcc agctttggcg caaacgccag aattgccaac agcggcgaca
     5761 atgtacacat cattgccagc ggtgaaaatt caacggtagt gagcacgggc gtggtcgact
     5821 caattattct cgggctaggg ggaagcgccg cactggcata tcatgacggc gaaagagttc
     5881 gcttcgccgt cgccattgaa ggtgaaaaca atattcgaac cggagtccgc tatcgtctta
     5941 atgaacagca tcagtttgtt gagtgctgat agctttacct ttaatcaaac gaaagacgcc
     6001 gacgagcacg ggatgcaggc tttacgaccg cccgagccat cgcctcacca atttctgcac
     6061 atgccgtaag tccctgaacg aaaggctggt cgtgcatcaa ccagggcaac gcgccgaacg
     6121 ctacgcgtcc acgaatatct tcgggttgct gtaacgtata atcttcgcca acatcaggga
     6181 tttcagatcc ccgcttcacg taaggccagt agtcgacgaa tagactctga agggattgcc
     6241 gcataattat cgatgaaaac cctggcaagg cctttactga accgtttatg gtcgtgttca
     6301 ttcagatgag gaacaatttc ctgtacggct tcatgcaggc gcagaatgac ataacgccag
     6361 ggaacagtat gtttttctcg tttattgcgt tcgacttcct ggagattttt ttctgcccag
     6421 tcaaatgggt cgcgttgttt gcgctcggca aaccaggctt gagcaaagga atcgacattc
     6481 aggctctcta aggctatgcg ttggctccag tctggatcag caaacttgat ttcctctact
     6541 atcaatctaa atacccgatc caaaaggccc tcttcacctt tttgaatctc agcatttaat
     6601 gcctgatcgg tgacgatgtg taagggctcg tagggaatag ggcaatagaa atcggcttcg
     6661 ggtaaaatac ccgtgcgcga catcaacgta atatttagct tttcacttgc gttatcgcgg
     6721 tgaaaaacga cgtgttgttt atcatcttca atgaacgaac catgctgaat agccactgcc
     6781 attgccgcat ccagtccgct caaggatgtt cccataatac ccacgttaca cgcatcgacc
     6841 tttgcttcca tcaggcctga ccacgggctg ggaaaatacg ttctggttgc ttcttcttca
     6901 tcaggccaga cgtgacccgt ggcaatcacc gctaaatcaa acgtctcgct gggtaaatcc
     6961 tgatttgtag cgagcatgac gccagcattt gtaatttgca gatcggtaac ctggcatgat
     7021 tcataaacag ccactgcaaa tttttgctgt cgtgcctggt ctactaatcg taaaaattga
     7081 tcgcggaaat attcgcccag cagaattcgc ggtaaaaact gacgatcgtg caaggtttct
     7141 tttttaacgc cataacgctg gagatggctg gcttcttgct tttgtagcca ttcgagatac
     7201 gtacaattaa tcggcggtat ttcaatactg gcaatatttg ccagcatcat ttttgagttt
     7261 tcctcatcac tgtatggcat cccgacaccg gcctcgtcag cctgctcgaa gatagaaata
     7321 gaaagtggag tttgttgctg tagaagcgag aataaggtgt agatccccgt aggcccggca
     7381 cccacaatag caattttttt cattgttact tacctgtacg cattctttcg aatttagtgt
     7441 tatcagataa gtttggttga gatttttaga atttgagaaa tttcagacag ggatgaaaag
     7501 ggcgctaacc gcccttgatt aaattctgtc aggcgagttt tgcctgattg atttgactaa
     7561 gtggctcatt gacatcgaaa actttggcga caacttcctc aacgccgaga tttttcagtc
     7621 gggcagtatg tttttccaga taggcaaggg cgcttttttc atcagtgaaa aggtagatcc
     7681 caccggcttc gtggttcttt tcactttctg tccatacctt ccacagaaaa ccaggttcct
     7741 gattaatcga ctcagcaagt ggtttaagct gctcagccat tgcgtcgcca aatgggccat
     7801 taaaagcaaa atgaagttgt aacaaagttg ccatagcgat tctccttctg ataacgcata
     7861 cgaagacagt gtaaatcttt taacgtattg gatacagaga agaaacagag ggaaaaagga
     7921 aaagaccaga ttaagaaaat ctggtgcgtc cgagtggact cgaaccaccg acccccacca
     7981 tgtcaaggtg gtgctctaac caactgagct acggacgcag gatggtgcgt tcaattggac
     8041 tcgaaccaac gacccccacc atgtcaaggt ggtgctctaa ccaactgagc tatgaacgca
     8101 acgttgtagg tgacaacggg gacgaatatt agcggcagag tggaaaggtg gcaagaggca
     8161 aaacgtaatt ttctgcgcta tttcgaccgt ttgcagagct tttaagcaaa ttggctatat
     8221 tttgttgatt tgcaagggcg atttttattc aggatcgcat ttacatctga tacaacccaa
     8281 taattcgtgt atcagagttt aaacagagtt gtttgccacc atctcaaatc tgtcgtggat
     8341 aaatcctccg tagcctttac acttaggtag caataaaggc acacagatgc agcaaaggaa
     8401 taaatcatgg gatctgatgc gaaaaacttg atgagcgacg ggaatgtgca aattgttaag
     8461 accggcgagg tccttggcgc gacgcaactt actgaaggcg agttgattgt tgaagctggc
     8521 ggaagagccg aaaataccgt ggtcacgggg gctggctggt tgaaagtggc aaccggtggg
     8581 atcgccaaat gcacacagta cggtaacaat ggcacgctat cggtcagcga cggtgccatt
     8641 gccacagata ttgttcagtc cgagggaggc gcaattagtc tctctacgct cgctacggtt
     8701 aatggccgcc atcccgaagg tgaattcagc gttgatcagg gttatgcctg cggtttgttg
     8761 ctggaaaatg gcggtaacct gcgtgtactg gaaggacatc gcgcggaaaa aatcattctc
     8821 gatcaagagg gcggcctgtt ggttaatggg acaacctcag cggtcgtggt agatgaaggt
     8881 ggtgaattgt tggtgtatcc aagtggagaa gccagcaatt gtgagattaa tcagggcggc
     8941 gtttttatgc tggccgggaa agccagtgat acgttgcttg ctggtggcac catgaataat
     9001 ctcggtggtg aagactctga cactattgtt gagaatggat ccatttatcg tctggggacg
     9061 gatggccttc agctctacag ttccggtaag acgcaaaacc tgtcggtgaa tgtggggggg
     9121 cgggctgaag tgcatgccgg tacgctggaa aatgcggtaa tacaaggtgg aacagtgatc
     9181 ctgttgtcac ccaccagcgc ggacgaaaat tttgtcgtag aggaagatcg cgcaccggtt
     9241 gaactgaccg ggagtgttgc attactggac ggcgcttcaa tgattattgg ttatggcgca
     9301 gagctgcaac aatcaacgat tactgtacag cagggcggtg tgttgattct cgacggcagt
     9361 acggtaaaag gtgacagcgt cactttcatt gttggtaaca tcaatctgaa tggcggaaaa
     9421 ctgtggctga tcactggtgc ggcaacgcat gtgcaactga aagtgaaacg cctgcgcgga
     9481 gagggagcga tttgcctgca aaccagtgcg aaagaaattt cacctgactt catcaatgtg
     9541 aaaggggaag ttaacgggga tatacgcgtt gagataacag atgccagtcg gcaaactctg
     9601 tgtaacgctc tgaaattaca gccagacgaa gacgggattg gcgcaacgct ccagcctgcg
     9661 taaaaacaca ggcgcaaccc gtgaaggttg cgccttgtct ttattagcgg gacgctcgtt
     9721 gcagaatgat ggctgacggc atacgttgca ggaaccgcat acgcagcatc atcataatgg
     9781 ctgccgatgt caggccgata ataaagccta tccagaagcc tgctggcccc ataggttcaa
     9841 cgaccagatc ggtcagtgcc agaatatagc cgcttggcaa gcccagcacc cagtaagctg
     9901 taaaggtgat atagaaaatg gaacgcgtat ctttataacc acgcaaaatc ccactgccaa
     9961 tcacctggat tgagtcagaa atctgatata ccgccgccag caacatcaaa tgcgcagcca
    10021 gcgttacaac ctcgggattg tcgttgtaca acagggcgat ttgctcccgc agtgaaaccg
    10081 tgaaaatggc cgtcagggtt gccatacaga cacccaccat aagcccggtc cgcgcagcgg
    10141 tttgcgcatc cagcgttgag ccctgaccca gacgataacc tacgcggata gttaccgctg
    10201 ccgccagcga cattggaagc acaaacatca gtgaactaaa gttcagggca atctggtgtc
    10261 ctgcgacatc aacaataccg agcggagaca ctaacagagc cacgacggca aacagtgtca
    10321 cttcaaagaa cagtgccagc gcaatcggca aaccgagttg aatcagtcgt ttcataaccg
    10381 caggctcggg cttagcggtg cctttttcgt tacgaatatc gcgcatggag cgggcgcgtt
    10441 taatgtaaga aaccatggca aggaacatga cccaatacac cgccgcagta gccacgccac
    10501 aaccaacgcc accgagctca ggcataccga aatgaccata aataaagata tagttcaccg
    10561 ggatgttcac cagcaggccg ataaagccca ttaccatacc cggcttggtt tttgccagac
    10621 cttcacattg gttacgggca acctggaaga acagatatcc cggcgcgccc cacaacaacg
    10681 cacgcagata acccacggct ttgtccgcca gagccggatc gatgttttcc atgtagcgga
    10741 taatgtaacc tgcattccac agcaccagca taatgagaac ggaaacaaaa cctgccagcc
    10801 agaaaccttg tcgcacctga tgcgcaatgc gctcacgtcg accggaacca tttaattgcg
    10861 cgataaccgg cgttaatgcc agcagcagtc cgtgaccaaa gaggatcgcc ggaagccaga
    10921 tagaagtacc gatagcgacc gccgccatgt cggtggcact atagccgccc gccatcacgg
    10981 tatcgacaaa acccatcgca gtttgggcga tttgcgcgag aatcaccggg attgctaatg
    11041 ctaataacag acgcgcttca ctgatatact tctgcacgtg aacacctttt atttgtagtt
    11101 atatgagaga ctaaaaagcc gcctgaatgg gcagcaaaaa gaagaagcag gggaaattca
    11161 gtctattgta gcgaggattt attaattctc cagtgaaaaa atagccattc ttaccccttc
    11221 actggtaagg gcgttttcca cctgctattg tgctgaacag aatgtcttaa ctgatttcag
    11281 gagttgtaag tatgtttacg gggattgtac agggcaccgc aaaactggtg tcgattgacg
    11341 agaaaccaaa ttttcgtacg catgtggtgg agttacccga ccacatgctg gacggcctgg
    11401 aaaccggtgc ttccgtggcg cataacggtt gctgcctgac cgtgacggaa attaacggca
    11461 accatgtcag ttttgacctg atgaaagaaa cgttacgcat taccaatctt ggcgatttaa
    11521 aagtggggga ttgggtaaac gttgagcgtg cggcgaaatt cagtgatgaa attggcggac
    11581 acttaatgtc aggtcatatt atgaccaccg ctgaagtggc gaaaatatta acctcggaaa
    11641 ataatcgcca gatctggttt aaagtccagg atagtcagtt gatgaaatat atcctgtaca
    11701 aaggatttat tggcatcgac ggtattagcc tgaccgtcgg cgaagtcacg ccaacgcgtt
    11761 tttgcgtcca tttaattccg gaaacactgg aacgcacgac tcttgggaag aaaaaacttg
    11821 gcgcacgcgt caacattgaa atcgatccac aaactcaggc agtggtagat acggtagaac
    11881 gtgtgctggc ggcacgagaa aatgccatga atcaaccagg tacagaagcc tgatctgaac
    11941 tcgcccccga agagggggcg atagaatagc ctttagcgag cgactcgaag gccgttttca
    12001 acaccgcgtg agaacaccac ctgccagagc tgaatatcac gggcgcggaa agcacctgcg
    12061 caggcattca gataataggt aaacatccgt ttaaagcgtt cactgtagtt atccgcaatt
    12121 tctggccatg cggcgaggaa tcgttcatac cacgccatca acgtagtatc gtaatcagca
    12181 ccgaagttat gccagtcttc catcacaaag tggggttcgc tggattgagc aatctggcgt
    12241 acagagggca ggcaaccgtt cggaaaaata tatttattaa tccagggatc aacattcaga
    12301 tcggtttttt tcgaaccgat agtatggagc aggaatatac cttccggttt caaattacga
    12361 tccaccaccg caaaataggt atcgtaattt ttcggtccga cgtgctcgaa catccccaca
    12421 gaaacaatac gatcaaactg gtcgttcagg tcacgataat cttgcagcaa aatggtgaca
    12481 tccaggcctt tacagcgttc ctgagccatt ttttgctgtt cggcagaaat ggtgacgccg
    12541 accacgctta cgtcatagtt agatgccatg tagtgcgcta gtccgcccca gccgcagcca
    12601 atatccagta cgcgcatccc tggttttaac tgcaattttt cacaaatcat tttgagcttc
    12661 gcctgctggg cagattccag attatcggca tctttccagt aagcgcagga atattgcatg
    12721 aagggatcaa gcatgcggct gaacaagtca ttacccaaat cgtaatgctc cttgccgact
    12781 atccaggcac gttttttact ctgcagattg aagagacgag cgccggcaat acgcagcgtg
    12841 tctttgaaat gatgggggag ttggttctcg agacctgcgc gtaagacttt gctaaaaaac
    12901 atatccagtc ggtcacattc ccaccagcca tccatataac tttcgcctaa ccccaaagag
    12961 ccttcctgca gaacgcgttt aaaaaaatcg gggtttttca cacgaatatc cgccggggca
    13021 gaaccgttaa tggctatacc ggcacggcta agtaattcgt tggcgatacg gtaccagttg
    13081 tcatccggta cactgacttc ttctatacac gatgaactca tagtttctcc atcactggtc
    13141 ggtgatcaga accttaaaac agcgtagacg ctttttgggc tttgtgagaa atctcgcaga
    13201 aaaaaccgcg agtctataat ccgacgtaga acagagggaa aggaggaacc cttgccgaaa
    13261 tgaccatcca tagtgagtcg ggaacgatcc tgtccccgta aaaacataat atttatcgta
    13321 gctttaacaa ctcgaaatta gtataggccg ctaatttcaa tcatacaaca aaattttgtt
    13381 agcagcctaa gtataagtat atcgatatag atcagtgtga ttcgctatga gcgacttcgg
    13441 cattgccatg attcgggcag tcaacttctt cacaacgttg catcatgtaa cccagcgcga
    13501 ccagcactac tgttgataac atcacgctgg tggtggtgag caatggcgtg ctgatactaa
    13561 taagccagga aactaccaga cttgcgagga agcacagacc cagttgcaga gtgttctgca
    13621 acgctgcggc gcgaccggtt gcatgtggga agggacgcag cgcctgggcg acaacaatag
    13681 ggtagatcgc gccattggca atcgccatca cacagaatgg gatcaggatt tcgaccagcg
    13741 acacatggct aatgaagccc gcagcccagg tcgcaatgac gctgacagca aacagcacca
    13801 gcaaccacgg taataactgc ttgccttgcc atttctgcag tgcggcgcga cagccataac
    13861 caccaatcag aaacgcgata gtttgcggga cataacttaa accaataact gccgggctgt
    13921 agcccatttc actaaggatg aacggtgaac cggtcagcca tgcaaaaaaa ctggctgaac
    13981 aggctgcgta tatcagcacg ttgccgcgat aggttttaga acgtagcagg tcggtaaagg
    14041 tcagaccatc ctgactattg ttacgggcct tcgtcgtggg tttgagccag aaaataggca
    14101 gaatcagcac cacggtaatg gcaaacaggg tggcgaaaat cgcctgccag gaaaaatgga
    14161 ccagcagcca gcttcctaac aggggagcca gtgccggaga cagacccacc agcggcatga
    14221 tggtcgcaaa aatacggttg actttctgtg aaggataata atctgtcact aatgcttgcc
    14281 agataaccgc cgcggcgcag acccccacag cctgtacaaa acgcaatacc agcagcgtgg
    14341 cggcgttttc tacccacagc atccccagac tacctaacgc aaaaattgtc aggccgatta
    14401 ataacaccgg tttacgacca taacggtcgg agagtggccc ccacagaagc tgggctgcgg
    14461 caaaaccggc aaggaacaga ctaaggctgg cactgacagc agacgcaggc gtttgcaggt
    14521 cggcctgtat ggcggcgaaa gcaggcagat acatatcggt tgccagaaaa ccgagtacgc
    14581 tcaaacccgc cagccagact aaaaatcttt tcccaggttg catgtatatt ctctaaatct
    14641 ctcaattaaa caccgccgca aagtgtaggg agtgaaatta gtgttgtgaa acggtaatat
    14701 ttgatgactg gtttcaaaaa ttttgcaggc agactatgtg gtcagaatat tcactcgaag
    14761 ttgttgatgc ggtagcgcgt aatggtagtt ttagcgctgc ggcacaggag ctgcatcgtg
    14821 ttccttctgc ggtcagctat accgtgcgtc agctggaaga gtggctggcg gtaccgctct
    14881 ttgaacgacg gcaccgtgat gtggagctga ccgctgctgg agcgtggttt ctcaaagaag
    14941 ggcgctctgt tgtcaaaaaa atgcagatca cccgccagca atgtcagcag atagcgaacg
    15001 gctggcgcgg tcagttagct atcgcagtgg ataatattgt caggccagaa cgtacacggc
    15061 agatgatcgt tgatttttat cgccattttg atgatgtcga acttcttgtc tttcaggaag
    15121 tgttcaacgg tgtctgggat gcgctttccg acgggcgcgt ggaactggca attggcgcaa
    15181 cacgcgcgat tccggtaggc ggtcgttatg ccttccggga tatggggatg ctaagctgga
    15241 gttgcgttgt tgccagtcac cacccgctgg cgttgatgga tggcccgttc agcgatgata
    15301 cgttgcgcaa ctggccgtcg ttggtgcgcg aagacacctc gcgaacgcta cctaaacgta
    15361 ttacctggtt gctggataac caaaaaagac ttgtcgtacc tgactgggaa tcatcggcaa
    15421 cctgtatctc ggcaggatta tgcataggga tggtgccaac acatttcgcc aaaccgtggc
    15481 ttaatgaggg gaagtgggta gcacttgaac tggaaaaccc cttcccggat tcggcatgtt
    15541 gtctgacatg gcagcaaaat gatatgtcgc ctgcgttaac ctggctgctt gagtatctca
    15601 gcgatagtga gacgctgaat aaggagtggc tgcgggagcc ggaagagact cccgcaacgg
    15661 gtgattaacg acgatagtcg cggaacgggc cgtcagccac ggagcggcgt tcaatcaaac
    15721 gcggatgcac ttcaatggac tgcggttctt cacgtttgtt gacgatacga tccaacagca
    15781 tgttgaacgc tgtttcaccc agcgaatctt ttggctgatg gatcgtggtc agcgccggcg
    15841 taaaatagcg cgcgttgcgc acgttatcat aaccgatcag cgaaacatcc tgcgggacgc
    15901 gcaggcccat ttcatcagca gcacaaagtg cgcccattgc catgatatcg ccaccacaga
    15961 agacggcagt agggcgatgc ggctgcgaca ggatttgctg catggcgcga taaccggatt
    16021 caggttcaaa gtcaccctgc acaatccagc tttccggcac cttgatcatc gcttcttcca
    16081 tcgccttcat aaaaccggca aggcggcctg cgccggtgtt acgttccagc gggccgggga
    16141 tgacgccgat ttcgcggtga ccgcgttcaa tcagataacg cccggccatg tagccgcctt
    16201 cgaacgcgtt atcaatgacc gcatcggtga agtcagcttt tgcttcaccc cagtccatca
    16261 ccaccattgg gatatggcga tactcttcca gcatcgccag caacggctct gggtactcag
    16321 aacacatcac cagcagacca tcgacgcgtt tttgcgccat catcgacaga taagcccgct
    16381 gtttctcaag attgttccac gcattaccca gaatcagggt gtaacctttc tggaagcaat
    16441 ttttttcaac tgcttcaatg atctcggcaa aataggccgc ttcgctgctg gtcgccagca
    16501 aaccgataga cttggtgtgg ttaaccttca ggctacgcgc caccgcgcta ggggagtagt
    16561 gtaattcttt aatcgctgcc cacacggcgt tgcgcgtttc ttcagcgacg aaacgtgttt
    16621 tgttgatcac gtgtgacaca gttgtagtgg aaacgtttgc tcgtttcgct acatctttta
    16681 ttgttgccat tccatttcac tccagaccct aacttcagct cctgcaaaat cgcaaggtaa
    16741 acgtttgcct ttacacacct tacgcacaac aaaagtgttg cggtacgccg gaaaaacgac
    16801 acggagcgtc aggaaggggt caatggccag tacgcaaata gtgtaagcgt ggaattttgg
    16861 ccgatcttga cgaaaagggg aagtggtaaa aacacttatc attataaatg acgggagatt
    16921 ttttcatcac agtgtgtaaa aatgcgtaat atcacttttt gtgtggtaat aaaaaaggag
    16981 aaaacttgat gagcaccgac cttaaatttt cactggtaac aacggttatc gtcctcggtt
    17041 tgatcgtagc cgtgggtttg actgccgcgc tgcactgatt tcctctcgag ggagcgcgtt
    17101 ctccctcgca tctccttcct gtttgttagc aaaatggcaa aagtgttttc tctttttgtt
    17161 attttcattt ttttgtgatt gctgtcatgc tttcgccact ttaatgaata tcgcgtaatg
    17221 acgcgaagag gtggagataa ggcatgaaaa ttaactatcc gttgctggcg ctggcgattg
    17281 gcgcgtttgg tatcgggaca acggagttct cgccaatggg cttgttgccc gtcattgcgc
    17341 gcggtgtgga tgtctcgatt cccgctgccg gaatgttaat cagtgcctat gcagttggcg
    17401 taatggtggg cgcgccgctg atgacgcttc tactttctca tcgtgcccgc cgcagtgcgt
    17461 tgattttcct gatggcaatt ttcacgctag gcaacgttct ttccgccatc gcgccggatt
    17521 atatgaccct gatgctttca cgtattttga ccagcctgaa tcacggagca ttttttggtt
    17581 tgggttcagt cgtggccgca agcgtggtgc caaaacataa acaggccagc gcagttgcca
    17641 ctatgtttat ggggttaacc ctggcaaata tcggtggcgt gccggcggcg acctggttgg
    17701 gtgaaaccat cggctggcgg atgtcatttc tggcaacggc ggggctggga gtgatttcaa
    17761 tggtaagtct gttcttctca ttacctaaag gtggtacagg ggcacgacct gaagtgaaaa
    17821 aagagctggc ggtattaatg cgtccgcagg tgctgtctgc attgctgacg acggtactgg
    17881 gagctggtgc aatgtttacc ctctacacct atatctctcc ggtactgcaa agtattaccc
    17941 acgcaacacc ggtgttcgtc acggcaatgc tggtgctgat tggtgtcgga ttctctatcg
    18001 gtaactatct cggcggcaaa ctggcagatc gttcagttaa cggcacgttg aaaggctttt
    18061 tgttgctgct gatggtgatt atgctggcaa tcccgttcct ggcccgcaat gagttcggcg
    18121 cagctattag catggtggtg tggggcgcag caacctttgc ggttgtacct ccgttacaga
    18181 tgcgcgtgat gcatgtcgcc agtgaagcgc caggtctgtc ttcatcagtc aatattggtg
    18241 cctttaatct tggaaatgcg ctgggagcag ctgctggtgg tgcggtaatt tccgctgggc
    18301 tgggatacag ctttgtgccg gtgacggggg cgattgtcgc gggactggca ttattgctgg
    18361 tgtttatgtc agccagaaaa caacctgaaa cagtttgcgt tgctaacagc taattaaaca
    18421 aaaacgcaga aggataacct tctgcgttac agttgctgac aacgtgcgat ttatcttgcc
    18481 gaatgcgctg ttaacgcctc atccgcgttg gcatggtaag cgaagcgcat caggcaatgc
    18541 tgcatttgcc atcagttatt atgcagcgag atttttcgct acgaattccc agttcaccag
    18601 cgcccagaag tgctccagat agccaggacg tgcattgcga tagtcgatgt aataagcgtg
    18661 ttcccagaca tcaacggtca gcagcggagt cgcatcggtg gtcagcggag tacccgcgtt
    18721 agaggttgaa acgatagcca gtttgccatc gctgtttttc accagccagg tccagccaga
    18781 accaaagttt ttgatcgctg catcagtaaa ctgcgctttg aaatcggcaa agctgccaaa
    18841 agatgcggcg atagcttcag cgacttttcc agtcggttcg ccaccggcgt tcggtgccag
    18901 gcagttccag tagaaagtat ggttccagac ctgagctgcg ttgttgaata cgccaccttc
    18961 agagctgcga ataatctctt ccagtgattt accttcaaac gcggtacctt taatcaggtt
    19021 gttcaggtta gtgacataag tctgatgatg cttgccgtag tgatactcga tagtttccgc
    19081 agaaatgtgc ggtgccagag catctttagc atatggtagt gcaggtaatt cgaatgacat
    19141 tgctactctc ctttattatt aatttgcata cgtacaatag ccttattgtg cgtatggata
    19201 gggtagcaaa ttaaaaggtg agacaaaaga tgaacttacc ctgttgccgt aacaacaggg
    19261 taaagtttta gcgaagtgtt tttggggtca ttacccgacg agcgccaaca tagtggcgct
    19321 gccagtagtc ttcactgaga gaagtgattt gaatttcctg acctgtgcgc ggtgactgaa
    19381 taaatttgcc gttgccgaca tacacgccga catgatcggc tgtaccgcgt ccctgagtac
    19441 ggaaaaagac caggtcgccg tttttcagct cactacgttc gataggtcct gcatcacgca
    19501 ggtgatacat ttcattcgcc gtacgcggaa tacgaatttt caccaaatct ttataagcgt
    19561 aataaaccag gccgctgcaa tcaaaaccgg tacgcggtga gctgccgccc caacgatatg
    19621 gcttaccaat ttgctgcatc agtttattca ttgccacttt cgtggctttt tgcaccttcg
    19681 ctttgtgcgc gtcggcaagg gttcctgacg cggcattttt gactttcaca caatgcgatt
    19741 tatagccctt acgtttggta cacttttcgg taaagctggc tgaggccgtt ttattgacgg
    19801 cgtgtttgct gcgagttttg gcgtttttag aagcggtaat ggaagatttt ttaacagaag
    19861 aagcggtttt gcttttggtc atacttttac tggcggcttt tttcgctgtt ttctggctgg
    19921 ctttctgggt ctttttggtg gttgccgtgc ttttcttttt atctgctttt ttggtgatat
    19981 gagtggtagc agaactctcc ctggcttgct ttgaagcatg ggccataggc gttaaaggca
    20041 gggtggtaaa aagtaaagca cagagcgtga tcgaaatacg gtttatccgc gccactgagc
    20101 gtccctctgt taaggcaggt ctatcatgcc tgcgtctgat ttacaaaact tgatgattct
    20161 aatgcataga agaagagaaa gttaatagct tttattgtta aaaaatacgt ttcgttggcg
    20221 agcgcaaaaa aatgcagcag tgaaagctaa aaatggttat aaatacgcca gtttgccgcg
    20281 catcagagaa gaaacgaata tttgaaatgc aacaaataga gcaaagggat aaaatagcaa
    20341 aagcgtgatg aaaatgttat tttccgatgc cgcactgacc cgctacaatg tcagattact
    20401 gccgtaaaga agtttgagga agcaagacaa tgagcaccac tatcgaaaaa atccaacgcc
    20461 agattgctga aaacccgatc ctgctgtaca tgaaaggttc accgaaactg ccgagctgcg
    20521 gtttctctgc ccaggcagtc caggcgcttg ccgcatgtgg cgaacgtttt gcctatgttg
    20581 atattctgca gaatccggac attcgtgcgg aactgccgaa atatgctaac tggccgacct
    20641 tcccgcaact gtgggttgac ggcgagctgg tcggcggttg tgatatcgtg atcgaaatgt
    20701 atcagcgtgg cgaactgcag cagctgatca aagaaactgc cgctaaatac aagtctgaag
    20761 agccggacgc ggaataatcc gtcctgcaat actgacggat gcgaacgggc atccgtcagt
    20821 cctttatccg gtgcgccgta aaacctcgtc cttcagggcg gggatataag gcgcggtttt
    20881 ccacctgacc aggtgtttgc tgttgttcaa tgtactggcg gatgacggat attggcgcac
    20941 cttcgcagct actggcaaaa tagccaggac tccacaaaac gcctttgtag taatacctga
    21001 ctgcaatatc tggtcgatct cgtcgcagta acctattcga tatgcctttg aggctgttta
    21061 ccagactgga tatcgccagt ttgggaggat agttaattaa caaatggacg tgatctggtt
    21121 cgccatccat ttcaaccagt tcagctgcaa aatcagcaca tacatttgaa aagtaagtgc
    21181 gtagtttttc tgtcgtgtca tggtcaaaaa tctggcgtcg gtatttggtg acaaagacca
    21241 ggtgaacatg catcaggaaa acacaatgcc ttccacgccg gatatcggtt tcttttttca
    21301 cagaccaaag tatcatttga cctgtgaaac gattacaggc atttaaattc cagttaagac
    21361 tcggtggtca acaggagcgt gaaatgaggc gcttcgcagg cgcatgtcgt ttcgttttca
    21421 atcgtgctct ggcgcttcag aatgagaatc atgaggccgg taataaatac atcccttacg
    21481 ggaaaatggc ttcctggctg gttgagtgga aaaacgccac tgaaacgcaa tggcttaaag
    21541 attcgccctc acagccattg caacagtcac tgaaagacct tgagcgggct tacaaaaact
    21601 tcttccagaa gcgggctgct tttccccgat tcaaaaagcg gggacagaat gctgcattcc
    21661 gctacccgca ggacgttaag ctcgatcagg aaaacagccg tatttttctg ccgaaacttg
    21721 gctggatgcg ctaccggaat agccgtcagg tcacgggagt tgtgaaaaat gtcactgtca
    21781 gccagtcctg cggtacgtgg tacatcagta ttcagacaga aagtgaagta tcaactccgg
    21841 ttcacccttc agcatcaatg atcgggctgg atgctggcgt ggctaaactc gccacgctgt
    21901 cagatggcac agtctttgag cctgtaaaca gttttcaaaa aaaccagaag acgctggcga
    21961 gacttcagcg acagttaagc cggaaggtca aattcagcaa caactggcag aagcagaaac
    22021 gcaaaataca gcgactgcat tcctgtatcg caaatatccg cagggactac cttcacaaag
    22081 tcacaacgac cgtcagcaaa aaccacgcaa tgatagtcat tgaggatttg aaggtcagca
    22141 acatgtcaaa gtcagcagcg ggtacggtca gccagccggg gcgcaatgtc cgggcaaaat
    22201 caggtttaaa ccgttcgata ctggatcagg gctggtatga aatgcgccgc cagcttgagt
    22261 acaa
//
LOCUS       DAZSNO010000053        22019 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZSNO010000053 DAZSNO010000000
VERSION     DAZSNO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22019
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.053"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..169)
                     /locus_tag="ACHWN2_001982"
     CDS             complement(<1..169)
                     /locus_tag="ACHWN2_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR8931530.1"
                     /translation="MLARDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQI"
     gene            complement(179..730)
                     /gene="ybcL"
                     /locus_tag="ACHWN2_001983"
     CDS             complement(179..730)
                     /gene="ybcL"
                     /locus_tag="ACHWN2_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR8931531.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(899..1231)
                     /gene="emrE"
                     /locus_tag="ACHWN2_001984"
     CDS             complement(899..1231)
                     /gene="emrE"
                     /locus_tag="ACHWN2_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR8931532.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1576..1890)
                     /gene="fliE"
                     /locus_tag="ACHWN2_001985"
     CDS             complement(1576..1890)
                     /gene="fliE"
                     /locus_tag="ACHWN2_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR8931533.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2104..3763
                     /gene="fliF"
                     /locus_tag="ACHWN2_001986"
                     /pseudo
     CDS             2104..3763
                     /gene="fliF"
                     /locus_tag="ACHWN2_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            3756..4751
                     /gene="fliG"
                     /locus_tag="ACHWN2_001987"
     CDS             3756..4751
                     /gene="fliG"
                     /locus_tag="ACHWN2_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR8931534.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4744..5430
                     /gene="fliH"
                     /locus_tag="ACHWN2_001988"
     CDS             4744..5430
                     /gene="fliH"
                     /locus_tag="ACHWN2_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR8931535.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5430..6803
                     /gene="fliI"
                     /locus_tag="ACHWN2_001989"
     CDS             5430..6803
                     /gene="fliI"
                     /locus_tag="ACHWN2_001989"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR8931536.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6825..7166
                     /gene="fliJ"
                     /locus_tag="ACHWN2_001990"
                     /pseudo
     CDS             6825..7166
                     /gene="fliJ"
                     /locus_tag="ACHWN2_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            7163..8290
                     /locus_tag="ACHWN2_001991"
     CDS             7163..8290
                     /locus_tag="ACHWN2_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR8931537.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8395..8859
                     /gene="fliL"
                     /locus_tag="ACHWN2_001992"
     CDS             8395..8859
                     /gene="fliL"
                     /locus_tag="ACHWN2_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR8931538.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8864..9868
                     /gene="fliM"
                     /locus_tag="ACHWN2_001993"
     CDS             8864..9868
                     /gene="fliM"
                     /locus_tag="ACHWN2_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR8931539.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9865..10278
                     /gene="fliN"
                     /locus_tag="ACHWN2_001994"
     CDS             9865..10278
                     /gene="fliN"
                     /locus_tag="ACHWN2_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR8931540.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10281..10646
                     /gene="fliO"
                     /locus_tag="ACHWN2_001995"
     CDS             10281..10646
                     /gene="fliO"
                     /locus_tag="ACHWN2_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR8931541.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10646..11383
                     /gene="fliP"
                     /locus_tag="ACHWN2_001996"
     CDS             10646..11383
                     /gene="fliP"
                     /locus_tag="ACHWN2_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR8931542.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11393..11662
                     /gene="fliQ"
                     /locus_tag="ACHWN2_001997"
     CDS             11393..11662
                     /gene="fliQ"
                     /locus_tag="ACHWN2_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR8931543.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11671..12456
                     /gene="fliR"
                     /locus_tag="ACHWN2_001998"
     CDS             11671..12456
                     /gene="fliR"
                     /locus_tag="ACHWN2_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR8931544.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            12741..13364
                     /gene="rcsA"
                     /locus_tag="ACHWN2_001999"
     CDS             12741..13364
                     /gene="rcsA"
                     /locus_tag="ACHWN2_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR8931545.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13408..13596)
                     /gene="dsrB"
                     /locus_tag="ACHWN2_002000"
     CDS             complement(13408..13596)
                     /gene="dsrB"
                     /locus_tag="ACHWN2_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR8931546.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13759..13986
                     /gene="yodD"
                     /locus_tag="ACHWN2_002001"
     CDS             13759..13986
                     /gene="yodD"
                     /locus_tag="ACHWN2_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR8931547.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14284..15099
                     /locus_tag="ACHWN2_002002"
                     /pseudo
     CDS             14284..15099
                     /locus_tag="ACHWN2_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(15096..16790)
                     /gene="dgcQ"
                     /locus_tag="ACHWN2_002003"
                     /pseudo
     CDS             complement(15096..16790)
                     /gene="dgcQ"
                     /locus_tag="ACHWN2_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(16961..17143)
                     /locus_tag="ACHWN2_002004"
     CDS             complement(16961..17143)
                     /locus_tag="ACHWN2_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR8931548.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(17222..18139)
                     /locus_tag="ACHWN2_002005"
     CDS             complement(17222..18139)
                     /locus_tag="ACHWN2_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR8931549.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18312..19232
                     /gene="yedA"
                     /locus_tag="ACHWN2_002006"
     CDS             18312..19232
                     /gene="yedA"
                     /locus_tag="ACHWN2_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFR8931550.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19221..19691)
                     /gene="vsr"
                     /locus_tag="ACHWN2_002007"
     CDS             complement(19221..19691)
                     /gene="vsr"
                     /locus_tag="ACHWN2_002007"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR8931551.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19672..21090)
                     /gene="dcm"
                     /locus_tag="ACHWN2_002008"
     CDS             complement(19672..21090)
                     /gene="dcm"
                     /locus_tag="ACHWN2_002008"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR8931552.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21157..21658)
                     /locus_tag="ACHWN2_002009"
                     /pseudo
     CDS             complement(21157..21658)
                     /locus_tag="ACHWN2_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(21737..21994)
                     /gene="drpB"
                     /locus_tag="ACHWN2_002010"
     CDS             complement(21737..21994)
                     /gene="drpB"
                     /locus_tag="ACHWN2_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR8931553.1"
                     /translation="MIRGDSLWAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLG
                     STAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         5401 a         5801 c         5592 g         5225 t
ORIGIN      
        1 ttatctgatt attatcaaca gttagactat gcgtctccca attgataact ttttttccct
       61 gacggatacg acataaagct ggcataagag gcttaaccct gtgaatctca tgatgtttat
      121 gcatccgtat ttcttcaatc tttaggttag acttatctct tgccagcata ctcttaccct
      181 attttatctc ataaactggt gttatctcag cggttgcaat tttattggca ttaagcatat
      241 aaccaactaa cgctccgctg gaattagaat ccacaggaat cttatcagtt tttagagccc
      301 atactttaaa ctggtaatga tggggtttat ctcctttggg aggacatgct ccaccaaatc
      361 ctgcataacc aaaatcattt cggccttgaa cagcaccagt cggaagtttt gtaccatcac
      421 gtcttcctgc atcagtgggc aaatatgtta cagttgctgg aatattagca acagtccagt
      481 gccaccatcc actgcctgta ggagcatctg gatcatacac agttacggca aagcttttgg
      541 tgccttcagg agcaccagac caggttaatg agggcgatgt attaccacct tcacacccaa
      601 atccagaaaa tacatgagac gttgtaagtt gctctcctgt ttttatttca ttactagtga
      661 cctgaaatgc tgcggcctgc gcagaaaatg ttatgaatgc caatacactt gaaacgataa
      721 gttttttcat aaaaacctca ttgttgtgac ctatcgttat tttatttgat gtgccttgat
      781 ctcattatgc ataaaggcgc aatgatcgcg caaatgcaat cataattata aaggtgcccc
      841 gaacattagc cataatatgc acaaatcaga ttcctgttat ctcaaggaga attatttttt
      901 aatgcggtgc acttcgtgac aacaagttaa tgactaacac accggcacaa atcaacatca
      961 tgcctatgat agctggcagg tccagtcgtt gaccaaaaaa tccccaggaa agcaaactaa
     1021 tcagaacgat accgactcct gaccagatag cataagcaat ccctgtaggt atataagcca
     1081 gcgtctgagc taataaccag aatgatgcac aatagcaaat aattgtgcca acagagggcc
     1141 ataaccgtgt aaacccctct gaaaacttca ttaaggttgt accaatgacc tctgcaagta
     1201 ttgcgccacc aagataaata taagggttca tagcatattc tttcctgttc atactggaga
     1261 gaattgtact acagtttgaa cacaactcac cgttttcatc ctggtgtatg tgtatccaag
     1321 gggctctggc gtggcaagag ataaaggcag gaacaataat gatcagaagg aaccccaggc
     1381 taacgaatga gttcactctt cctgggagca aagcgaagac aaaacatgtt atcgcgttta
     1441 cggatcgtgg atggcagaaa acaaccagga tcaggtatcc attttaaacc agaaattgag
     1501 tgagtttgcc ccatccatgc cccacgctgt gggatcggat ataattaaac aagcatagtc
     1561 aacaggttac acaacctaca cctgcatgga catcacttcc tgatacgccg ccaccagctt
     1621 attacgcacc tgaatcccca tttgcataga aactgaggct ttttgcatat cggtcatcac
     1681 atcgtttagc gccacgccgg gttcaccgag ggtgaatttt tctgcctgcg tgcgggcgac
     1741 tgtttgtgta tcgcttatgc gatcgagcgc ggcgtgcagc tgcccggcaa aactaatggt
     1801 cggttgcggc agtgattcct gcgcacgcgc actcatcgcc gtagcctgta actggctgat
     1861 aaccccttca atcccctgta tcgctgacat tttcgtctcc cggataattt ctggtaacaa
     1921 agcctaccag taagtcaata agacaaaggc gctaaatagc aacaaaaaaa ctggtttatt
     1981 ggcggataga aaaaacgaaa gcacaaataa tgggagcgtc aatttttcga gtgtgctgac
     2041 ccgagagtga gtcttgttcc actttgccaa taacgccgtc cataatcagc cacgaagtgc
     2101 gcgatgaatg cgactgcagc ccagacaaaa tctcttgagt ggcttaatcg cctgcgtgcg
     2161 aatccgaaaa ttccattgat tgttaccggt tccgcggcag tggcggtcat ggtcgcactg
     2221 atcctgtggg cgaaagcccc cgactaccgc acattattca gcaatctttc cgatcaggat
     2281 ggtggcgcaa ttgtcagcca actgacgcaa atgaatattc cttaccgctt cagcgaagcc
     2341 agcggcgcta ttgaggttcc ggcagataaa gttcacgaac tgcgcctggc gcaacaaggt
     2401 ttgcccaaag gcggcgcggt cggtttcgaa ctgctcgatc aggaaaagtt tggtatcagc
     2461 cagttcagcg aacaggtgaa ttatcagcgg gcgctggaag gcgagctttc tcgtaccatc
     2521 gaaactatcg gcccggtaaa aggggcgcgc gtacatctgg caatgccgaa accgtcttta
     2581 ttcgtccgtg aacaaaaatc cccttctgca tcggtgacgg taaatctgtt acccggccgc
     2641 gcactcgatg aagggcaaat tagcgctatt gtacatctgg tttccagcgc ggttgctggt
     2701 ctgccgcctg gaaacgtcac gctggtggat cagggcgggc atctgttaac ccagtccaat
     2761 accagcgggc gcgatcttaa tgacgctcag ttgaaatatg ccagcgatgt cgaaggccgt
     2821 attcagcagc gtattgaagc gatcctgtca cctattgttg gtaacggtaa tattcacgcc
     2881 taggtcacgg cgcaactgga cttcgccagt aaagaacaaa cggaagaaca gtatcgccct
     2941 aacggtgatg aatctcatgc ggcgcttcgt tcacgccagc ttaatgagag cgagcaaagc
     3001 ggttccggtt atccgggcgg cgtaccgggg gcgttgtcga atcaaccggc acctgcgaat
     3061 aacgcgccaa tcagcacgcc tccgacaaat caaaataacc gccagcagca ggcgagcacc
     3121 accagcaata gtgggccgcg tagcacacag cggaatgaaa ccagtaacta cgaagtcgat
     3181 cgcaccattc gtcataccaa aatgaacgtg ggcgatgtgc aacgtctgtc agtcgcggta
     3241 gtggtgaatt acaaaacctt gccagacggt aaaccattgc ctctcagcaa cgaacagatg
     3301 aagcaaattg aagctctgac ccgcgaggcg atgggctttt ctgaaaaacg cggtgattcg
     3361 ctcaatgttg ttaactcgcc gttcaatagc agtgacgaaa gcggcggagc actgccgttt
     3421 tggcaacagc aagtgtttat cgatcagttg ctggctgccg gtcgctggtt gctggttgct
     3481 ggtactgctg gtggcgtggt tgctgtggcg gaaagcggta cgcccgcagc taacacgtcg
     3541 cgccgaggcg gtgaagactg tgcagcaaca ggcgcaggcc cgcgaggaag tggaagatgc
     3601 ggtggaagtc cgtctgagca aagacgaaca actccaacaa cggcgcgcta accaacgact
     3661 gggggcagaa gtcatgagcc agcgtatccg tgaaatgtct gataacgatc cgcgcgtggt
     3721 ggcgctggtc attcgccagt ggataaataa cgatcatgag taacctgaca ggcaccgata
     3781 aaagcgtcat cctgctgatg accattggcg aagaccgggc ggcagaggtg ttcaagcacc
     3841 tctcccagcg cgaagtgcaa accctgagcg ctgcaatggc gaacgtcacg cagatctcca
     3901 acaagcagct aaccgatgtg ctggcggagt ttgagcaaga agctgaacag tttgctgcac
     3961 tgaatatcaa cgccaacgat tatctgcgtt cggtattggt caaagctctg ggtgaagaac
     4021 gtgccgccag cctgctggaa gatattctcg aaacccgcga taccgccagc ggtattgaaa
     4081 cgctcaactt tatggagccg cagagcgccg ccgatctgat tcgcgatgag catccgcaaa
     4141 ttatcgccac cattctggtg catctgaggc gcgcccaggc cgccgatatt ctggcgttgt
     4201 tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc cacctttggc ggcgtgcagc
     4261 cagccgcgct ggccgaactg accgaagtac tgaatggctt gctcgacggt cagaatctca
     4321 agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat tatcaacctg atgaaaacgc
     4381 agcaggaaga agccgttatt accgccgtgc gtgaattcga cggcgagctg gcgcagaaaa
     4441 tcatcgacga gatgttcctg ttcgagaatc tggtggatgt cgatgatcgc agcattcagc
     4501 gtctgttgca ggaagtggat tccgaatcgc tgctgatcgc gctgaaaggg gccgagcagc
     4561 cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc cgccgatatt ctgcgcgacg
     4621 atctcgccaa gcgtggtccg gtgcgtctgt cgcaggtcga aaacgaacag aaagcgattc
     4681 tgctgattgt gcgccgtctt gccgaaactg gcgagatggt gattggcagc ggcgaggata
     4741 cctatgtctg ataatctgcc gtggaaaacc tggacgccgg acgatctcgc gccaccaccg
     4801 gcagagtttg tgccaatggt cgagtcggaa gaaaccatta ttgaagaggc cgaacccagc
     4861 cttgagcagc aactggcgca actgcaaatg caggcccacg agcagggtta tcaggcgggg
     4921 attgccgaag gtcgccagca aggtcacgaa cagggctatc aggaaggact ggcccagggg
     4981 ctggaacaag gtctggcaga ggcgaaggct caacaagcgc caattcatgc ccggatgcag
     5041 caactggtca gcgaatttca aactaccctt gatgcgcttg atagtgtgat agcttcgcgc
     5101 ctgatgcaga tggcgctgga ggcagcacgt caggtgatcg gtcagacacc aacggtggat
     5161 aactcggcac tgatcaaaca gatccaacag ttgttgcagc aagaaccgtt attcagcggg
     5221 aaaccacagc tgcgcgtaca cccggatgat ctgcaacgtg tggatgatat gctcggcgca
     5281 actttaagtt tgcatggctg gcgcttgcgg ggcgatccca ccctccaccc tggcggatgt
     5341 aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg gcaagaactc
     5401 tgccgtctgg cagcaccagg agtggtgtaa tgaccacgcg cctgactcgc tggctaacca
     5461 cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc tacgggcgat
     5521 taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg ctcggcgcaa
     5581 cctgtgtcat tgagcgccag aacggcagcg aaacgcacga agtagaaagc gaagtcgttg
     5641 gctttaacgg tcaacggctg tttttaatgc cgctggagga agtggaaggt gtcctgcccg
     5701 gcgcgcgtgt atatgccaaa aacatttcgg cagaagggct gcaaagcggc aagcagttgc
     5761 cgctcggtcc ggcgttatta ggtcgtgttc tggacggtag cggtaaaccg ctcgatggcc
     5821 tgcccttccc cgatacgacg gaaaccggtg cgctgattac cccgccattt aacccgttgc
     5881 aacgtacacc gattgaacat gtgctggaca ccggcgtgcg cccaatcaat gccctgctta
     5941 ccgtcggacg cgggcagcgt atggggctgt ttgccgggtc gggcgttggt aaaagtgtgc
     6001 tgctggggat gatggcacgc tacacccgcg ccgatgtcat tgtcgtgggt ttgattggtg
     6061 aacgtgggcg cgaagtaaaa gattttattg agaatatcct cggtgccgaa ggacgtgcac
     6121 gctcagtggt gattgccgct ccggcggatg tttctccgct cctgcgaatg caaggtgccg
     6181 cctatgccac gcgcattgcc gaagattttc gcgatcgtgg tcagcatgtg ttgctgatta
     6241 tggactccct cacccgctac gcgatggccc agcgtgaaat tgcgctggcg attggcgaac
     6301 cacctgccac taaaggttat ccaccgtcgg tgtttgccaa attaccggca ctggtcgagc
     6361 gtgccggtaa tggcattagc ggcggcggct cgattaccgc gttttatacc gtgctcaccg
     6421 aaggcgatga ccagcaggac cccattgctg actccgcgcg ggcaatcctc gacggtcaca
     6481 ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc ggctatcgat attgaagcgt
     6541 cgatcagtcg cgcaatgacg gcgttgatca gtgagcaaca ttacgcgcga gtgcgcacct
     6601 tcaaacagct gttgtcgagt tttcagcgta accgcgatct ggttagcgtc ggcgcgtatg
     6661 ccaaaggcag cgatccgatg ctcgataaag ccatcgccct gtggccgcag ctggagggct
     6721 atttgcaaca aggcattttt gaacgcgcgg actgggaagc gtctctccag gggctggagc
     6781 gtattttccc gacagtgtca taacccagga gataacggca gatggcggaa gaacagctca
     6841 aaatgttgat cgattatcag aatgaatatc gcaataacct caatagcgat atgagtgccg
     6901 ggatgaccag caaccgctgg atcaactatc agcagtttat ccagacgctg gaaaaagcca
     6961 ttactcagca tcgccagcaa cttaatcagt ggacgcagaa agttgacatt gccctgaaca
     7021 gctggcgaga aaagaaacaa cgtttgcagg cctggcagac actgcaggaa cggcaatcca
     7081 cggcggcact gcttgcagaa aaccgcctcg atcagaaaaa gatggatgag ttcgcccagc
     7141 gcgccgccat gaggaaacct gaatgattcg cttagcgccc ttgattaccg ccgacgttga
     7201 caccaccaca ttgcctggcg gcaaagccag cgatgctgca caagattttc tcacgttgtt
     7261 gagcgaagca ttagcaggcg agacaacaac tgacaaagcg gccccccagt tgctggtagc
     7321 aacagataag cccacgacaa aaggcgagcc gctggtcagc gatattcttg ccgatgcgca
     7381 acaagcggat ttactgatcc ctgtggatga aacacttcct gtcatcaacg acgaacaatc
     7441 cacatcaaca ccgttaacca ccgctcagac gatgacgttg gctgcggtgg ctgacaaaaa
     7501 tacgacaaaa gacgaaaaag cggatgatct taatgaagac gtcaccgcaa gtctgagcgc
     7561 cctttttgcg atgttgccgg gttttgacaa tacgcccaaa gtgactgatg caccgtcaac
     7621 cgtgttaccg gcagagaaac caacgctctt cacaaaactg acttctgcgc aactcactac
     7681 agcacagcct gatgacgccc ccggcacacc agctcagcca ttaacaccgc tggtagcaga
     7741 agcccagagt aaagcggaag tcatcagcac gccttcgccg gtgaccgctg acgccagccc
     7801 gctaatcact ccacaccaga cacagccact gcccaccgtc gccgcgcctg ttttgagtgc
     7861 accgctgggt tctcacgaat ggcaacaatc attgagccag catatttcgc tgttcacccg
     7921 ccaggggcaa caaagtgcag agttgcgtct gcacccacag gatttaggtg aagtgcaaat
     7981 ttccctcaaa gtggatgata accaggcgca gatccagatg atttcaccgc atcagcacgt
     8041 acgcgccgcc ctggaagcag cgctgccggt actgcgtacg cagctggccg aaagtggcat
     8101 tcagttaggg caaagcaaca ttagtggcga aagctttagt ggtcagcagc aggccgcttc
     8161 ccagcaacag caaagccaac gcacagcaaa ccatgaacct ctggcggggg aagacgacga
     8221 tacgcttccg gttcccgtct ctttacaagg gcgtgtaaca ggtaacagcg gcgttgatat
     8281 tttcgcctaa cgtcagaggt agcaccgtaa tccgcgtctt ttccccgctt tgttgcgctc
     8341 aagacgcagg ataattagcc gataagcagt agcgacacag gaagaccgca acacatgact
     8401 gattacgcga taagcaagaa aagcaagcga tcgctttgga tcccgattct ggtattcatt
     8461 accctcgcgg cctgtgccag cgcaggttac agctactggc attcgcatca ggttgccgct
     8521 gacgacaaag cgcagcaacg cgtcgtgccc tccccggtct tctatgcgct ggataccttt
     8581 acggtcaatt tgggcgatgc ggatcgcgtg ctttatatcg gcataaccct gcgactgaaa
     8641 gatgaagcaa cccgctcgcg gctgagtgag tatttgccgg aagtccgtag tcgcttgctg
     8701 ttactgtttt cacgtcagga tgctgccgta ctggcgacag aagaaggcaa gaaaaacctg
     8761 attgccgaga ttaaaaccac actttccacc ccgcttgttg ccgggcaacc gaaacaggat
     8821 gtcaccgacg tgctgtatac cgcttttatt ctgcgataac gacatgggcg atagtattct
     8881 ttctcaagct gaaattgatg cgctgttgaa tggtgacagc gaagtcaaag acgaaccgac
     8941 agccagtatt agcggcgaaa gtgacattcg tccgtacgat ccgaataccc aacgacgggt
     9001 tgtgcgcgaa cgtttgcagg cgctggaaat cattaatgag cgctttgccc gccattttcg
     9061 tatggggctg ttcaacctgc tgcgccgtag cccggacatt accgtcgggg ccatccgcat
     9121 tcagccgtac catgaatttg cccgcaacct gccggtgccg accaacctga acctaatcca
     9181 cctgaaaccg ctacgcggca cagggctggt agtgttctcg ccgagtctgg tgtttatcgc
     9241 cgtggataac ctgtttggcg gcgatggacg cttcccgacc aaagtggaag gtcgcgagtt
     9301 tacccatact gaacagcgcg tcatcaaccg tatgttgaag ctggcgctgg aaggctatag
     9361 cgacgcctgg aaagcgatta atccgctgga agttgagtac gtgcgttcgg aaatgcaggt
     9421 ggaatttacc aatatcacca cctcgccgaa cgacattgtg gttaacacgc cgttccatgt
     9481 ggagattggc aacctgaccg gcgaatttaa tatctgcctg ccattcagca tgatcgagcc
     9541 gctacgggaa ttgttggtta acccgccgct ggaaaactcg cgtaatgaag atcagaactg
     9601 gcgcgataac ctggtgcgcc aggtgcagca ttcacagctg gaactggtgg ccaactttgc
     9661 cgatatctcg ctacgcctgt cgcagatttt aaaactgaaa cccggcgacg tcctgccgat
     9721 agaaaaaccc gatcgcatca tcgcccatgt tgacggcgtc ccggttctga ccagtcagta
     9781 cggtactctc aacggtcagt atgcgttacg gatagaacat ttgattaacc cgattttaaa
     9841 ttctctgaac gaggaacagc ccaaatgagt gacatgaata atccggccga tgacaacaac
     9901 ggcgcaatgg acgatctgtg ggctgaagcg ttgagcgagc aaaaatcaac cagcgaaaaa
     9961 agcgctgccg acgcggtgtt ccagcaattt ggcggtggtg atgtcagcgg aacgttgcag
    10021 gatatcgacc tgattatgga tattccggtc aagctgaccg tcgagctggg ccgtacgcgg
    10081 atgaccatca aagagctgtt gcgtctgacg caagggtcag tcgtggcgct ggacggtctg
    10141 gcgggcgaac cgctggatat tctgatcaac ggttatttaa tcgcccaggg cgaagtggtg
    10201 gttgttgccg ataaatatgg cgtgcggatc accgatatca ttaccccgtc cgagcggatg
    10261 cgccgcctga gccgttagtg atgaataacc atgctactgt gcaatcttcc gcgccggttt
    10321 ctgctgagcc actgctgcag gtgagcggcg cactcatcgc cattattgcc ctgatcctcg
    10381 ctgctgcctg gctggtaaaa cggctgggat ttgcccctaa acgcactggc gttaacggtc
    10441 tgaaaattag cgccagtgct tcgctgggcg cgcgtgaacg ggtcgtggtg gtcgatgtgg
    10501 aagatgcacg gctggtgctc ggcgttaccg cagggcaaat caatctgctg cataaacttc
    10561 ccccttcagc accaacggaa gagataccgc agaccgattt tcagtcggtc atgaaaaatt
    10621 tgcttaagcg tggcgggaga tcctgatgcg tcgtttgttt tctgtcgcac cggtccttct
    10681 ctggctgatt acgcccctcg ccttcgcgca actgccgggt atcaccagcc agccgctacc
    10741 tggcggtgga caaagctggt cgctcccggt gcagacgctg gtgttcatca cctcgttgac
    10801 gttcattcca gcgattttac tgatgatgac cagtttcacc cgcatcatta ttgtttttgg
    10861 tttattgcgt aacgcgctgg gaacgccctc cgcgccacct aaccaggtat tgctggggct
    10921 ggcactgttt ttgacctttt ttattatgtc gccagtgatc gacaaaattt atgtcgatgc
    10981 gtaccagcca ttcagcgaag agaaaatctc aatgcaggag gcgctggaaa aaggggcgca
    11041 gccgctgcgt gagtttatgc tgcgtcagac ccgcgaggca gatttagggt tgtttgccag
    11101 actggcgaat accggcccgt tgcaggggcc tgaagctgtg ccgatgcgca ttttgctccc
    11161 ggcctacgtg accagcgagt tgaaaaccgc atttcagata ggcttcacga ttttcatccc
    11221 ttttttgatt atcgacctgg tgattgccag cgtgttgatg gcattgggga tgatgatggt
    11281 tccccccgcc accattgctc tgccctttaa actgatgctg tttgtactgg tggatggctg
    11341 gcaattgctg gtcggttcgc tggcgcagag cttttacagc tagagaggca aaatgacacc
    11401 tgaatcggtc atgatgatgg gaactgaagc gatgaaagtc gcgctggcac tggcagcccc
    11461 gctgttgctg gtagcgctgg tcacgggcct tatcatcagt attttgcagg cagccacgca
    11521 gattaacgaa atgacgctgt cgtttattcc gaaaatcatc gccgtattta tcgccatcat
    11581 tattgccgga ccgtggatgc tcaatctgtt gctggattac gtccgcacct tgttcactaa
    11641 cctgccgtat atcatcgggt agcccgtaca atgatgcagg agacaagcga tcaatggctt
    11701 tcctggttaa gcctgtactt ctggccgtta ctgcgcgtgc tggcgctcat ttctaccgcg
    11761 ccaattctga gcgaacgcag cgtgccgaaa cgggtaaaac tgggtctggc aatgatgatc
    11821 acgttcgcca ttgccccatc attacctgcc aacgatgttc ctgttttttc gttctttgct
    11881 ctgtggctgg ccgtacagca gatcctgatc ggcattgcgc ttggttttac catgcaattt
    11941 gcctttgccg ctgtgcgaac cgctggcgaa attatcggtc tgcaaatggg gctgtcattt
    12001 gcgacatttg tcgatcctgg cagccatctc aatatgcccg ttttagcgcg catcatggat
    12061 atgctggcat tactgctgtt cctgacattt aacggtcatt tatggttgat ttcactgctg
    12121 gtcgatacct ttcacaccct gccgattggt agcgaaccgt tgaacagcaa tgcgtttctg
    12181 gcacccacca aagcagggag tttgattttc cttaacgggc tgatgctggc gttaccgctc
    12241 attactctgt tgctgaccct gaatctggca ttaggtttac tgaatcgtat ggccccgcaa
    12301 ttatccattt ttgttattgg atttccatta actctgactg tcggcatctc tttaatggcg
    12361 gcattaatgc cgttaattgc gcctttttgc gaacatttat tcagtgaaat ttttaatttg
    12421 ctggctgata ttattagtga attaccatta atataattcc gtaacgttta tcatgctacc
    12481 ctaaggatta tccgaaaaat aatatctgcg aacatcttcc aggatactcc tgtagcgaaa
    12541 tatttgtttt aagctcactc acatatcgca acatttactt taagacaatt ccaggcaaat
    12601 tatacaacac tttacgggat agtaagtccg cctgaaaaat tgcgagagtg gcgcattagg
    12661 tgacccatgt tgttccgttt agtcatgatg aaatattcag gtaaggggaa ttatcgttac
    12721 gcattgagtg agggtatgcc atgtcaacga ttattatgga tttatgtagt tacacccgac
    12781 taggtttaac cgggtatctg ttgagtagag gggttaaaaa aagagaaatc aacgacattg
    12841 aaaccgttga tgaccttgcc atagcttgtg attcacagcg tccttcagtg gtgtttatta
    12901 atgaggactg tttcatccac gatgcttcta acagtcagcg catcaagcac atcattaatc
    12961 aacatcccaa tacgttattt atcgttttta tggcaattgc caatgttcat tttgatgaat
    13021 atctattggt cagaaaaaat ttattgatca gttctaaatc gattaaaccg gaatctctcg
    13081 acgatatcct tggcgatatt ctgaaaaaag agacaacgat aacctcgttt ttaaatatgc
    13141 cgacgttatc attgagccga accgaatcga gtatgttgcg aatgtggatg gcaggtcagg
    13201 gaaccattca aatctccgac caaatgaata tcaaagccaa gaccgtttca tcgcataaag
    13261 gtaatattaa acgtaagatc aaaacgcata ataaacaggt tatctaccat gtcgtccgac
    13321 tgacggataa tgtgactaat ggtatttttg tcaacatgcg ctaacacatt ctgactggtg
    13381 gtttcccacc agtcaggctg aataagatta ctctgctttc tcaacaaaga taccgtcctg
    13441 atgccctgct tcattaaaga accagatgcc gagcgggtag tcttccagcg caaccaggta
    13501 cattgtgcct tcactaaact cctcaactgc cagtaccact ccaggacgac gcggaccgcc
    13561 atccgttttg actgttaccc gatcattcac cttcatcgtt ttcctcctgt ggctttgtgc
    13621 cagtgtagaa caatttcgtt tttctggcag cgccaagcgc gcgcgagtgc tgattttctc
    13681 gacggtctat acttaagaga tgccaggcgg acttaacgac tggcggcaac aacagagtaa
    13741 cggttgcgag gaaagatgat gaaaaccgca aaagtgtaca gcgataccgc aaaacgtgag
    13801 gtcagcgtcg atgtcgatgc cctgctggcg gcgatcaatg aaattagcga aagcgaagtt
    13861 catcgcagcc agaacgattc tgaacacgtt agcgtcgatg gacgtgaata tcatacatgg
    13921 cgtgaattgg cggatgcctt cgaactggat attcatgact tcagcgtctc tgaagtgaat
    13981 cgttgaatgc acaataaaaa aatcccgacc ctgagggtgt cgggatgaaa cttgcttaag
    14041 caagaagcac ttaaaaaatt cgttacacca ggaaatctga tgtgttcatc accttatctg
    14101 caattttttt cgctgacaag aaaatattcg cgctatgaat gattaacgct acttatgaat
    14161 agaaatgtga cgcgcgtcac cccttggtgg ctctggtgtc aacgggatcg ctcccgaaaa
    14221 aatcatcttt gattatttgt gctgttttga gaatcatcct ggtaagggga gtactgcgcg
    14281 accatgtttt caattcaaca accactactg gtttttagcg atcttgatgg caccctgctg
    14341 gacagtcata gttatgactg gcaaccggca gccccctggc tcagccgttt acacgaagcc
    14401 aatattcccg tcattctctg tagcagtaaa actttagcgg aaatgctgta cttgcaaaaa
    14461 atgttggggc tacaaggctt accgctgatt gcagagaatg gcgcagtgat ccagcttgct
    14521 gaacaatggc aggatataga cggttttcca cgcatcatct caggtattag ccatggcgaa
    14581 atctgccagg ttttaaatac gctacgcgag aaagaacatt ttaaattcac gacttttgat
    14641 gatgtcgacg atgcaaccat tgccgaatgg acgggattaa gccgtagcta ggcggcgctg
    14701 acgcagctgc atgaggcgtc ggtaacgcta atctggcgcg acagtgacga gcgtatggca
    14761 caatttatcg ctcgtctgaa cgaactgggc ttgcagttta tgcagggtgc gcgcttctgg
    14821 cacgtactgg atgcctctgc cggaaaagat caggctgcca actggattat cgcgacctat
    14881 caacaattgt caggcagacg cccaaccaca cttggcctgg gcgatgggcc aaacgatgcg
    14941 cctttactgg aggtaatgga ttacgcggtg attgtgaaag ggctaaaccg tgaaggggta
    15001 catctgcatg atgaggatcc ggcccgcgtc tggcgaacgc agcgtgaagg accggaaggc
    15061 tggcgtgaag ggctggacca ttttttctcc gcccgttaag cgttatcgct cgcgaatacc
    15121 cgattgcgcc cggcctgttt tgcgaggtaa agccgacggt cggccaatga ctggagttgt
    15181 tcaaaatcat aatcaccggt ttcctcgctg ctacttaccc ccagcgaggc actgatgcgt
    15241 atcgttgtac tcttggcgat cagcatctct ttttcattta acttcaggcg aatacgttct
    15301 gcgacctccg cagcctgcgt cagattcgcg cctggcagaa tcacacaaaa ctcctcacca
    15361 ccgacccgcc cggcaacgtc ctgcgcacgc aaggaactgc taattaatcc ggcagcatga
    15421 gaaagaacac ggtcgcccgc ctgatgacca aagcggtcat taatcgcttt aaaatggtca
    15481 agatcgacct gaatgacaga aaaaggatgt tggtgcgtct gacacaattt agcgagcgga
    15541 cgggcttttt cgaacagtgc gccacggtta tataaacgcg ttaaggtgtc gtgccacgcc
    15601 tgccactgca acgagctttg cagaacatac atgttgctga ccatccggcg aatcacatac
    15661 caggagagga gtaacatggt ggtaaagagc gcccacagca gggttaatgc aatgctgata
    15721 ctgccgaaat cgccgcgcac gccttcgctt agcgtatgga cgcgcgccag cacaccgtcg
    15781 aaatggtcca gacgttccca gctaacatag cgactgtcca tacgaatgcc gccccgcgtg
    15841 tcatgttcca ttgcctgcgc cagcaaggcc agttcacgag gatcaaaaat attccctgtt
    15901 ggatggtcag gattggaaga ggtcaaaaat ctcagcttac tgtcatagag ctgatactca
    15961 ccatcgaggt ttttatcgat ggcgtttctt aaaaattgct gcatagtacg cacgggaata
    16021 ctcatcccca gcacgccata ccagtagtta ttactgtcta ccggaacact gacggtaacc
    16081 tgcggttcag tattgctggc gtgttccggt tgcgaagtga accagcgtac cgcgcggtga
    16141 cgattttctc gttgcgaatg gccgataaac cagggttggg tgacatagcc gtaataacgc
    16201 gttggtacat tgcgcgtaaa caaggtcggc tgcgtcgaaa cgtaaaatcc agcacgcgag
    16261 acatacatcg cctgttcaac catcgacgag gagttgtgcg ccagtcgcag caagtaacca
    16321 acttccagtg cagcggtaat ttcattgtcg aggctttcat tttcgcgaga caggaaattc
    16381 ccctcgctga ctaatgcatc cgatacgcca ttgactggca gggtgcgccg tcggttgagt
    16441 tcgatttgcc aggcgtgctc gtcgcgatgc tgttcgaact cggttacggc attacgcaga
    16501 gaggtgaaat ccagtggcgc cacgagagct tcgcgcatgc cattacgcaa aaagatcagt
    16561 ttgtcgacgt tatactgcaa atgcttatcg agcgcgttgg ctacgttttc cagatgatta
    16621 cgctagctgg agatataggc atcttccagc accaccactt cacgccaggt gagcaaggtg
    16681 gaaaaaagca ataccacgat aaagcagaga ttaacgacat gaccgggccc caggcggcgt
    16741 gcgagttttt tcaaccagct ctggttttcc atttttgtct cgtgctgcac cctgacgact
    16801 cccaacctgc ttttttatga ttctggcata acccggcaat agcgtaccgc aaaacagcga
    16861 taattttata atggtggcat gataagacgc gactatcatt cattgcaggc attgggacac
    16921 caggccggat gtgatccggc ctggtgttaa tgataccctt tcaaacttct tccgcagaac
    16981 gactcccctc ccccggaaca agatcggttt catgaaaagc ttcccgcttg accccgtaac
    17041 cgtcatacca acgacactca accataccgc tggagtatcc agtgacaatc atccgcgggc
    17101 cgccctcttt aaccgtaact tcctcactaa ccataaagct catactcgcc tccttttttc
    17161 gagtgaaact tgttcacctt agttgaagat ggcgaatttt gcataatagc cagtgcgagt
    17221 attaatgtgc ctgaccgcgc attttcgcta ctgctttcac tcccagcacc acgatgccgc
    17281 cgatgataaa tccaagaatc agatttaaaa cagtcggtaa tatcatcgcc accactgcac
    17341 tttgctgccc ggcgatatgt tcaatagcgt gatgcagcgg cgcaatacca tgtaccacaa
    17401 tcccgccgcc gacgaggaac atcgccagcg tgccgacaat cgataatgct ttcatcagcc
    17461 aaggcgcgat aatcaataac cctttaccta atgcctgcat cagcgcgcta gatttttccg
    17521 ccagccaata ccctaggtca tcaatcttaa cgataacccc taccagaccg tagacgccca
    17581 cggtcactac cagcgcgatg cctgaaagca ccagcacctg attaagcaac ggcgcctctg
    17641 ccacaatccc cagcgtgatg gcgacgattt ccgcagacaa aataaaatcg gtacgaatcg
    17701 cccctttaat tttgtccttt tcaaacttca gcggatcctg cgccgccagc ttctccagac
    17761 gctgctggct ctgcgctgga tcttctttat gtttacgcgc ctccagcata tgcagcactt
    17821 tctctacccc ttcaaagcag agaaacgcgc caccaatcat caacagtggc gtaatcgccc
    17881 acgggataaa cgcactgatg atcagcgcca gcggcaccag aatcacttta ttgatcagcg
    17941 atcctttcgc cacgccccag accacgggaa gttcccggtt ggcccgcacg cctgaaactt
    18001 gttgcgcatt gagcgataaa tcatccccta atacaccggc ggttttcttc gccgccagtt
    18061 tgcccatcac agagatatcg tccagcagtg tggcgatatc atcgagcaac gtcagtaaac
    18121 tactcccggc caaaagcatt ccctctcatt tttattgttg aagggtgtaa gtatgaagca
    18181 aaaacggcag ctctgatact tgcttcacag gtcaacattt atttaacaat caagtgcaat
    18241 tatatctctc gccagcagaa tgattttcac gtttactatg aaccaccttt ttatttccgc
    18301 cgtgagggat tatgcgtttc cgccagttgt taccgctttt tggcgcgctg tttgcgttgt
    18361 atatcatttg gggctcaacc tattttgtca ttcggattgg cgtgggaagc tggcctccgt
    18421 taatgatggc gggcgttcga ttccaggcag ccggtatttt attgctggca tttttgctac
    18481 tgcgcggaca caaacttccc ccgctacgtc cgctgctcaa tgccgcgctg attggcctgt
    18541 tattgctggc tgtcggtaat ggcatggtga cggttgccga acatcaaaat gttccttccg
    18601 gcatcgccgc cgtagtgatt gcaaccgtgc ccctctttac cctgtgcttc agtcgcctgt
    18661 ttggcattaa aacgcgcaaa ctggaatggg tgggtattgc cattgggctt gccggaatca
    18721 tcatgctcaa tagcggtgga aatttaagcg gcaatccgtg gggcgcgatt ctgattttaa
    18781 tcggctcgat tagctgggcg tttggctcag tttatggctc gcgcattacc ttacctgtag
    18841 ggatgatggc gggtgcgatt gagatgctgg cggcaggcgt ggtgttaatg atcgcatcga
    18901 tgattgcggg tgaaaaactg acggcgctcc cttccctgtc aggcttcctt gcggtcggct
    18961 atctggcgct gtttggttcg attatcgcca ttaacgctta tatgtattta atccgtaatg
    19021 tcagtccggc tctcgccacc agctacgcct acgttaaccc ggtggtcgcg gtcttgctgg
    19081 gtacgggact gggtggcgaa acgctgtcga agattgaatg gctggcgctc ggcgtaattg
    19141 ttttcgcggt ggtactggtc acgttgggaa aatatctctt cccggccaaa cccgttgtcg
    19201 cgccagttat tcaggatgca tcaagcgagt aaatgaatcc cctgcgtgtc gatctgcgcg
    19261 ctggcacctt cgccgcagat ccactcttcc agacgctcgg taagcgccgc atccgtcagc
    19321 ttctcgcgcc tacgtaacgc acactcccag acaatcagta cgcgccagcc gagctcctgc
    19381 aagcgactga tatcgcggcg atcgcgttca acatttttac ctatcttctc cagccagaac
    19441 tcagttcgag tcgcaggcac tttaaacaga tagcagtgat gatgatgcca gaagcagccg
    19501 tgggtaaata tcacgcagcg atattcatca acgacaaaat ccgggcgtcc gggcagactg
    19561 gcgtcctgaa cgcgaaacgc aaggccctgc ccggttaaca aactggcgag gcgcttctca
    19621 atcgccgtat cacgcgtggc aatcgcgcgc atatttttgc tgcgggtggc cttatcgtga
    19681 acgtcgacca tgttgtgcct cttgctgacg caacgccacc gcctgtttga tttttggctc
    19741 aagcagtttt gccaccgcgg caaagaccgg cacgaccacc gagttgccga actggcgata
    19801 ggcctgagtg tccgaaaccg gaatgcggaa tttcgcttct cccggcgctt caaaacccat
    19861 taagcgcgcg cattcacgag gcgttaaccg acgtggacga tgttgctgat tcagcggatc
    19921 gtcaaagtct ttctcacccg tggccatatc ccaaccgcga tcgattaaaa tttccgcgcc
    19981 atctttgtaa taacgagcag acagcgtacg ggtgacgctt tgcggattgt tcggataaac
    20041 cattccataa ccgaagccgt taccgcgcgc ctgatgtttt ttcgcatatc gatagaggta
    20101 cttccacagc accggcgtca ggatatattt cgcctcaacc atcgggtcca gcagctgcgc
    20161 cagcgtcact cgctgtgcag ggaaacattc gctgatatca cgcagagtaa aatcggcttt
    20221 cagattcaga tcgcgacgaa aacccaccag cacgatgcgt tcacggtgct gcggcaggaa
    20281 atgtttacca tcgatgattt tcggatcgtc cggcccgtta tcttctgcat cggcaacgtc
    20341 atagcccagt tcgtccagcg tctgcatgat gatgcggaac gtttttccct tatcgtgact
    20401 cttcaggttt ttgacgtttt cgagcacaaa catcgccgga cgacgcgcgt cgataatgcg
    20461 caccacatca aagaacagcg tgccctgggt atcgcaggca aaaccgtgcg cccgcccgag
    20521 cgagttcttt ttcgatacgc cagccagcga aaatggctga caagggaaac cggccagtaa
    20581 aacatcgtgt tcaggaatgt gttgacgaat atgttccgcc gccgcctcat cactcacgcc
    20641 ttcttgatga ctgagggtga tgtcgcggat atcttcatta aaatgatgcg tcgccgggtc
    20701 gcaataatgg ttggctttat aagtgcgtac cgcatgtttg ttccattcgc tggtaaacac
    20761 gcactgtcca ccaatcgatt caaaaccacg acggatgccg ccaattccgg caaatagatc
    20821 gataaagcga aacgcataat gcggatgatg ttccggtggt ttgggtaaca acgtttgcag
    20881 atgggcaaac tctttctcac ttaaacggtg ccatgccgag tcattcgcca gcgcacgttt
    20941 taaaattgcc gcgctccagt gattctcacc cacaccatta agctgcgcca ccagcatttt
    21001 aacatcataa atttgcagca gtttctccag cattgcctgt gcggcattcc cggtgctgta
    21061 tgaatcggtt actgatatat tttcctgcat agatttaacc ggccatctga gatggccgac
    21121 aggttaacat aattactgcc gtttagggcc acgcgctcag ccagcgggtg aaaacgcatc
    21181 tatcaccttg tgatcgatac cttcattctc ccccgccagt tcggcaccga gcttcgccat
    21241 caaacagcgc caagctcaat gcccctgaaa cggcaaatac gcgcgccagg ccaatcgccc
    21301 ccagggcttc cagccgatcc gcatcctgga cgattttagc ttccgttgtt aagggggcga
    21361 tttgcgcgct gaaactgtga gcggcaatgg catgacaaac ggcctcaatt ttctccgccg
    21421 gaaattgcac gaactcttca cggagcaggc gacgcgtctc ttctgccgcc aggattgaag
    21481 aacgctgccg ttgcggatga tttttcgcca ggctcacgat atcgtgaaaa taacaggcgg
    21541 ttaaaatcac cagcatatcg acatcgtcat ctgccgccag tttctgtgcc gtagcccaga
    21601 cacggcgaaa atgacacagg tcatgcgccg cgtcctgatg ttgatgatgg tttttcaacc
    21661 agttctcgaa ctatgcctgc cagtgttgta actccatacg cgctccgttg ccaaagagtc
    21721 tcaaccttag cagtttttat tcatagcgtc tgcaacgtgc gggacgaggt cgggtgtacc
    21781 atgcaatgcc gcctaacaag gcaccaacca gggccataaa cagaaatccg accaatgctc
    21841 ctaaccactt ccccgcggta gatcctaaat cactttcaaa tcccgacacg ggggcatcgg
    21901 ggatccggct catcacccag atgtatcgcg ccattgccca gacgaaatag ccacaccatg
    21961 tatagaaagc ccacaatgaa tcgccacgga taatctagac acttccgagc cgttgataa
//
LOCUS       DAZSNO010000054        21499 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZSNO010000054 DAZSNO010000000
VERSION     DAZSNO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21499
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.054"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(38..2107)
                     /gene="glyS"
                     /locus_tag="ACHWN2_002011"
     CDS             complement(38..2107)
                     /gene="glyS"
                     /locus_tag="ACHWN2_002011"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR8931554.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2117..3028)
                     /gene="glyQ"
                     /locus_tag="ACHWN2_002012"
     CDS             complement(2117..3028)
                     /gene="glyQ"
                     /locus_tag="ACHWN2_002012"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR8931555.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3123..3422)
                     /locus_tag="ACHWN2_002013"
     CDS             complement(3123..3422)
                     /locus_tag="ACHWN2_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR8931556.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3597..4592
                     /gene="wecH"
                     /locus_tag="ACHWN2_002014"
     CDS             3597..4592
                     /gene="wecH"
                     /locus_tag="ACHWN2_002014"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR8931557.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4634..5071)
                     /gene="yiaA"
                     /locus_tag="ACHWN2_002015"
     CDS             complement(4634..5071)
                     /gene="yiaA"
                     /locus_tag="ACHWN2_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFR8931558.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5117..5458)
                     /gene="yiaB"
                     /locus_tag="ACHWN2_002016"
     CDS             complement(5117..5458)
                     /gene="yiaB"
                     /locus_tag="ACHWN2_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR8931559.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5627..7081)
                     /gene="xylB"
                     /locus_tag="ACHWN2_002017"
     CDS             complement(5627..7081)
                     /gene="xylB"
                     /locus_tag="ACHWN2_002017"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR8931560.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7153..8475)
                     /gene="xylA"
                     /locus_tag="ACHWN2_002018"
                     /pseudo
     CDS             complement(7153..8475)
                     /gene="xylA"
                     /locus_tag="ACHWN2_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8841..9833
                     /gene="xylF"
                     /locus_tag="ACHWN2_002019"
     CDS             8841..9833
                     /gene="xylF"
                     /locus_tag="ACHWN2_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8931561.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9911..11452
                     /gene="xylG"
                     /locus_tag="ACHWN2_002020"
     CDS             9911..11452
                     /gene="xylG"
                     /locus_tag="ACHWN2_002020"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFR8931562.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11430..12611
                     /gene="xylH"
                     /locus_tag="ACHWN2_002021"
     CDS             11430..12611
                     /gene="xylH"
                     /locus_tag="ACHWN2_002021"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFR8931563.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12689..13867
                     /gene="xylR"
                     /locus_tag="ACHWN2_002022"
                     /pseudo
     CDS             12689..13867
                     /gene="xylR"
                     /locus_tag="ACHWN2_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(13975..14799)
                     /locus_tag="ACHWN2_002023"
     CDS             complement(13975..14799)
                     /locus_tag="ACHWN2_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFR8931564.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15119..17148
                     /gene="malS"
                     /locus_tag="ACHWN2_002024"
                     /pseudo
     CDS             15119..17148
                     /gene="malS"
                     /locus_tag="ACHWN2_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17326..18579
                     /gene="avtA"
                     /locus_tag="ACHWN2_002025"
     CDS             17326..18579
                     /gene="avtA"
                     /locus_tag="ACHWN2_002025"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFR8931565.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18730..19203)
                     /locus_tag="ACHWN2_002026"
     CDS             complement(18730..19203)
                     /locus_tag="ACHWN2_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR8931566.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19305..20153)
                     /gene="yiaJ"
                     /locus_tag="ACHWN2_002027"
     CDS             complement(19305..20153)
                     /gene="yiaJ"
                     /locus_tag="ACHWN2_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFR8931567.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20353..21345
                     /gene="yiaK"
                     /locus_tag="ACHWN2_002028"
     CDS             20353..21345
                     /gene="yiaK"
                     /locus_tag="ACHWN2_002028"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFR8931568.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRD
                     AKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21357..>21499
                     /locus_tag="ACHWN2_002029"
     CDS             21357..>21499
                     /locus_tag="ACHWN2_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFR8931569.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5402 a         5489 c         5277 g         5331 t
ORIGIN      
        1 agcctgccag atggcaggct ttttaataac ggcgttatta ctgcaacagc gaaatatccg
       61 caacgcgcag gaacagttcg cgcagtttct ccagcatggt cagacggttg agacgcaatt
      121 ctttgtcatc aaccatgacc atcactttat cgaagaaagc atcaaccggc tcacgcagct
      181 ctgccagttc gaccagcgca tcctggtaac gaccttccgc aaagtacggc tccagcttgt
      241 cacgtagcac cacaacctgc atcgccagtt taatttcttc cggctctttc agggtagagg
      301 cattcacgcg atcgctcagc acttcgtcag atttcgccag gatgttagat acacgtttgt
      361 tcgccgccgc cagtgctgca gctgcttcca gggtacggaa gtgcgatacc gctttcatgc
      421 gggcatcgaa atcagccgga cgagtcggac gacgcgccag taccgcctgg atggtgtcaa
      481 cggtataacc ttcgtcctga taccaggcgc ggaagcgacc gagcataaag tcgataacat
      541 catcaactac gttggcatta gtcagcttat cgccatacag acgcaccgct tcttcggtca
      601 gcgtttgcag atcaaggttg aggttcttct caacgataat tcgcagcacg ccaagtgcgg
      661 cacgacgcag cgcaaacggg tctttgtcgc ctttcggatg ctgaccgata ccgaagatac
      721 ccgccagagt atccatctta tcagcaatcg ccagcgcaca agctaccggg ttggacggca
      781 ggtcatcacc ggcaaagcgc ggctgatact gctcattcag cgcgacggct acgtcttccg
      841 cttcgccatc gtgacgcgcg tagtgcatcc ccataacgcc ttgggtgtcg gtgaactcga
      901 agaccatgtt ggtcatcagg tcacacttag acagcaggcc cgcacgggtt gcgtggttaa
      961 cgtcagcgcc aatctgttcg gcgatccagc cagccagcgc ctggatgcgg tcagtcttgt
     1021 cacgcagcgt acccagctgt tgctggaaca gcacggtttg caggcgcggc aggttatctt
     1081 ccagacgttt tttgcggtcg gtgttgaaga agaactcggc atccgccaga cgtggacgaa
     1141 cgactttctc gttaccagag ataatttgct gcggatcttt cgattcgatg ttggcaacga
     1201 agataaagtt cggcagcagt ttgccgtcgt tcgcatacac cgggaagtac ttctggtcac
     1261 ctttcatggt gtaaaccagg gcttcagccg gcaccgcgag gaatttctct tcgaattttg
     1321 cggtcagaac gaccggccac tccaccagcg aagccacttc ttccagcagg ctttcgctta
     1381 agtcagcgtt accgccaatc ttacgcgctg cttcttcggc atcggcttta atcttcgctt
     1441 tacgttcttc gtaatcggcg atgactttcc cgcgctcacg cagaatttcc ggatactgat
     1501 cggcgttgtc gatggtgaat tccggttcgc ccataaagcg gtggccgcga atcacgcgat
     1561 cggactgaat gcccagaatg gttgccggaa tgactttgtc gcccagcagc agggtcacgg
     1621 tgtgcaccgg acgcacgaag tgcacgtcgc ttgcacccca gcgcatcagt ttcgggatcg
     1681 gcagtttcgc cagcgaagtc gcaaccatat tcggcagcag tgcttcggtg ctttcgccct
     1741 tcacatgggc gcgatacagc agccattcgc ctttatcggt agtcagacgc tcagcctggt
     1801 caacggtaat accacaacca cgcgcccaac cttctgccgc tttgctcggt tttccttcag
     1861 cgtcgaacgc ctgggcaatc gccgggccgc gtttttcgat ttcacgatct ggttgtgctt
     1921 ccgccaggtt agctactttc agcgccagac gacgcggagc agcaaaccat tgaacggtgc
     1981 cgtgtgcgag gcctgcgtta tccagctccg cagtaaagtt cgcagcaaag gactctgcca
     2041 ggctgcgcag tgcttttggt ggcagctctt cagtgccgat ttccaccaga aaagttttct
     2101 cagacatagc cgcctcttac ttatctttgt tgcacatcgg gaagccgagg gcttcacggg
     2161 aagcgtaata tgcttctgcc actgctttgg tcagggtgcg aatgcgcaga atgtagcgct
     2221 gacgctcggt gacggagatg gctttacgcg catccagcag gttgaagctg tgggcggctt
     2281 tcagaatacg ctcgtaggct ggcagcggca gcggattttc cagcgccagc agctgctgcg
     2341 cttctttctc gtactgctca aagcaggtga acaggaagtc cacatccgcg tattcgaagt
     2401 tgtaagtgga ctgctccact tcgttctgat ggaacacgtc gccgtaggtg gttttaccca
     2461 gcgggccgtc gctccagact aggtcgtaaa cgctgtctac gccctgaatg tacatggcca
     2521 gacgttccag accgtaggtg atctcgccgg taaccggttt acactccaga ccaccaacct
     2581 gctggaagta agtgaactgc gtcacttcca tgccgtttag ccacacttcc cagcccagtc
     2641 cccaggcccc cagagtcggg ttttcccagt tatcttccac gaaacggatg tcgtgaatag
     2701 tcgggtccat gcccagctct ttcagagaac cgaggtacag ctcctgaata ttgtccggcg
     2761 atggcttaat gaccacctgg aactgatagt agtgctgtaa acggttgggg ttttcgccgt
     2821 agcgaccatc ggtcgggcga cgagaaggct gaacataagc agccgccatc ggttctggcc
     2881 ccagcgcgcg cagacaggtc attgggtgag aggttcccgc gccgacttcc atgtccaatg
     2941 gttgaacaat ggtgcagccc tggcgagccc agtaatcctg taaggtcagg atcaagccct
     3001 ggaaggtcct ggtatcaaac ttttgcatat tatttcgtgc tggatacgtg tggatttaaa
     3061 ggaagggatc agtatacccg ctggatggaa gatatacagt acgaaacggg aaaaagcagg
     3121 gcttaacgca tggaaagatg caaaaaatgg ccatctgcgt caaaagaaca gacaaactgc
     3181 tctttacgga aggtataacc gcgcatttca tagcttccct ggaactgttc gaaggcggtg
     3241 acgtcgattt tttgctcgcc ggtgttataa cgttttgccg cctgatcctt gcataacgct
     3301 tccatattca acgaacgcag aggatcaacc cttacccgtt gtgctttctg cacgggtgac
     3361 ggtgtagaac aacccactag caccataagc gccattgccg gaaagaaagc gttcatcatc
     3421 attaaattac cgtctggttt gcaggtgagt cttattattt atatcggtat agtggctgat
     3481 atcttacgaa tttccttaag cctctatagg ctgtagtgat aaaactcaga ttattccagt
     3541 gcgccaacca aatgatttat cggtcagact ttttttaacc agatacagag acacagatgc
     3601 agcccaaaat ttactggatt gataacctgc gagggatagc gtgtttaatg gtggtgatga
     3661 ttcacaccac tacctggtat gtgaccaatg ctcatagtgt tagccccgtc acatgggata
     3721 tcgccaatgt tctgaattct gcctctcgtg tcagcgtgcc gctatttttc atgatttccg
     3781 gctatctctt ttttggcgaa cgcagcgccc agccgcgcca tttcttgcgt atcggcttat
     3841 gtctgatttt ttatagcgca atcgcactgc tctacattgc gctgtttacc tccatcaata
     3901 tggagttagc gctgaaaaac ctgctgcaaa agccagtgtt ttaccacttg tggtttttct
     3961 tcgcgattgc ggtgatttat ctggtttcac cgctgattca ggtgaagaac gtcggcggaa
     4021 aaatgttgct ggtgctaatg gtggtgattg gcattatcgc taacccaaac acagtgccgc
     4081 agaaaattga cggttttgaa tggctgccaa ttaacttata tatcaatggc gatacttttt
     4141 actacattct gtatggcatg ttgggccgcg ctatagggat gatggacaca cagcataaag
     4201 cactgtcgtg ggtgagcgcc gcgctgtttg cgacgggggt ttttattatc tctcgcggga
     4261 cattatatga attgcagtgg cgcggaaatt ttgccgatac ctggtatctt tactgtgggc
     4321 cgatggtttt tatctgcgca atcgcgctat tgactctggt taaaaacacg ctggatacgc
     4381 gtaccattcg cggacttggc ttaatctccc gccattcatt gggtatatac ggattccacg
     4441 ccttgattat ccatgcgctg cgcacccggg gaattgagct taaaaattgg ccaatactgg
     4501 atattatttg gatcttttgc gcgacgttgg cagcgagttt gttactttct atgctggtac
     4561 aacgaatcga cagaaacaga ttagtgagtt aagtaaaagc ccgttcacat tggactgacc
     4621 gggcttacgt gaattattcg ctgtattctt cctgggtaac ctgtgtttct tttggcgggt
     4681 ttattccggc atcacgaata ttcttctgca ccgctactgc accaaacaag cttaagaaga
     4741 aagccagtcc ataaaaacct ttttcactga gtaataatgt cgcattccac agacctacca
     4801 tcagtagagc aacagagata ataaagacag tcaggcaggt catataataa atggaagtgg
     4861 tcggtatgcc ttcatacttg tcccgaacgg tcttttgata agacgcagca gaaaacagtc
     4921 ctaacaccag tacggcaaaa taataccctt tttcatttaa ctgcatctct gcattccata
     4981 gccctaacag gtaggtaacg ataccaccaa cgagagctat ccatgacaca atactaaagg
     5041 ccggtgaata ggttgatatt ttgttgtcca tcacagtatt cctttcattc ctgaatgtgt
     5101 aagagcttta tgttgctcat accgagcgaa acaaacgcat ttgaccaaac aagcagacaa
     5161 aaaaggcgat ggggtagatc accatttcat aagcattgat aggtgcattt aaaacaccga
     5221 taaacaagag accgatagtg attaacgcaa ctaactggca gatatgggta aaaaaattat
     5281 ccagttgccc gagtttttct gcgcgaagat atgcatagtt accaaaagtt gctgtcatta
     5341 acacgccaag aaaatagcct tttccactta gcaatgggca tgagatccat agcccaacca
     5401 gataaaccag cgccccgaca acaaataata gttttgccac agtttttgag gttttcataa
     5461 cgatctccat atctaccagc gatacattac gagtaaccaa cgaaagacaa aactgaaaaa
     5521 tgccattaac aaatgatttt cagaataaat tcatactaaa tattaattaa ttagtgagat
     5581 atatagatgt gaattatccc ccacccggtc aggcagggga taacgtttac gccattaatg
     5641 gcagaagttg ctgatagagg cgacggaacg tttcacgtcg tggctgataa gcggcataac
     5701 gctgcgcatc tggtagatgc gactgttcta acggtagctg cggcaacaac tcaatgagcg
     5761 atttctctgg attcgccgca atctgcgcca gccttgctgc gcccagtgct ggccccacat
     5821 ccccccccgt acggtaatcg agctgctgac cgctgatatc cgccagcatc tgacgccagt
     5881 actcactacg cgccccgcca ccaatcaacg taacactttg cggtttaatg ccgcaggcat
     5941 gcacgacatc catgccatct gccagcgcat aacccacgcc ttccagcact gctcgcgcca
     6001 gttcattggg gccatgttga tgagtcaaac caaagaaaac ccccttcgcc tggggattat
     6061 tgtgtggtgt acgctcgccg gaaagataag gcagaaacca aactggctcg gcactttcat
     6121 cagcctgttg agctgcagcg attaaagctg ggacattgca caggccggtt aatttcgcgg
     6181 cccaatccag acacgatgct gcactcagca ttacagacat taaatgccaa cgctgcggta
     6241 gcgcatggca aaagctgtgt acagcgcttt ctggcttgct taagaaccct tcgctgacag
     6301 caaaatagac ccccgacgtc cccagcgata acattgcctg attagcatca accattccca
     6361 caccaactgc accagctgca ttgtcgccac cgcctgcgac aactggcacc gtcgccatac
     6421 cccacgcttt cgcaacttca ggtaacaaag caccagtaat ttcgctgcct tcgtataatg
     6481 cgggcatctg gtcacgagat aagtcgcaag cctgcagcat gacgtcactc cagtcacgct
     6541 ttgcgacatc cagccacatg gtgccagctg cgtcagacat atcgctggca aactcccccg
     6601 tcatacgcag acgcaagtaa tctttcggta ataatacttt gtcgatttga cggaatatct
     6661 ccggctcatg ccgctgaacc catagcaatt taggcgcagt aaatccgggc atcatcaggt
     6721 tgccggtaat cactcgtgat tgcggaactc tcgcttccag caaagtgcac tcttgcgcac
     6781 agcgcccgtc gttccacaaa atggcagggc gcaatacccg ttgttgagca tccagtaagg
     6841 ttgctccatg catctggccg gcaataccca atgctttaac gtcctgcaga gaatgctgat
     6901 cgcccagagc tttcattgcg cgatcagttg cctgccacca ctgttccggg tcttgttccg
     6961 accaaagtgg atgcgggcgc gaaacggtca gcttttccgt ttgcgaagca accacctcac
     7021 cctgctcgtt gagcaaaata acttttacgc ccgaggtgcc aagatctatc ccgatataca
     7081 tatcgatcgt tccttaaaaa aatgcccggt atcgctaccg ataaccgggc caacggactg
     7141 cacagttagc cgttatttgt cgaacagata atgatttacc agattttcca gttgctcctg
     7201 gcgaccactc tgatgcaccg gagacaaatt atgttcctga gcatatttgg ctaaatctgc
     7261 cagtgacatt tggcctttca ggatttgctg gcccaattcg ctattccagc cggaataacg
     7321 ctgcgcgatg cgtttatcca gctcgccatc ttcaatcatg cgcgctgcaa ttttcagcgc
     7381 cagtgccatc gtatccatcg cgccgatatg accgtaaaac agatcatatt tatcagtact
     7441 ttgacgacgt actttggcat cgaagttcag accaccggtg gtgaaaccgc ctgctttgag
     7501 aatttcatac atcaccagcg cattctcttc cacactgttc gggaactggt cggtgtccca
     7561 gcccagttgc gcatcgccac ggttggcgtc gacagaaccg aacaggccaa gcgcaatggc
     7621 ggtggctatt tcatgatgga aagagtgacc tgccagcgtc gcgtggttag cttcaatgtt
     7681 cagtttaatc tctttttcca gaccaaactg tttcaggaag ccatagaccg tcgcggcatc
     7741 gtaatcatat tgatgtttag tcggttcttg cggtttcggt tcgataagca acgtgcccta
     7801 gaagccgatt ttatgtttat gctcaaccac catctgcata aagcggccca gttgttcacg
     7861 ctcctgacgc aagtcggtat ttaacagcgt ttcgtaacct tcacgaccac cccacaggac
     7921 atagttttca ccgcccaatt tatgggttgc ttccatcgct gtaacaactt gcgttgccgc
     7981 ccagctgaag acttcaggat ctgggttcgt cgccgcaccc gcgccatagc gagggtttgt
     8041 aaagcagtta gcggttcccc acagcagctt cacgccgctc tcttcttgct tggctgccag
     8101 gacatcaacc atttgcgcaa aattattgat gtactctttt aacgacgcgc cctcagggga
     8161 aacatccaca tcgtggaagc aataaaatgg cacatgtaac ttgtggaaaa actcaaatgc
     8221 gacatctgct ttacgcttcg ccaacgccag tgcctcacca ggctgctgcc acggacgatt
     8281 aaacgccccc acaccaaaca tgtccgcccc gttccagcag aaggtgtgcc agtagcaggc
     8341 ggcaaaacgc aagtgctctt ccatacgctt acccaacacc agttcgtcgg gattgtagtg
     8401 acggaatgct aacgggtttg aggattttga gccttcataa cgaacgcgat cgagctggtc
     8461 aaaataggct tgcatattga actccataat caggtaatgc cgcgggtgat ggatgatgtc
     8521 gtaatattgg gcactccctt tcaattgctc aattatgtta tttcacactg ctattgagat
     8581 aattcacaag tgtgcgctcg ctcgcaaaat aaaatgggat gatgaaaatg ggtaattcct
     8641 cgaagataaa aatgcaataa gtacaattgc gcaacaaaag taagatctcg gtcataaatc
     8701 aagaaataaa ccaaaaatcg taatcgaaag ataaaaatct gtaattgttt tcccctgttt
     8761 agttgctaaa aattggttac gtttatcgcg gtgattgtta cttattaaag ctgtcctcta
     8821 actacagaag gccctacacc atgaaaataa agaacattct actcaccctt tgcacctcac
     8881 ttctgcttac caacgttgcg gcacacgcca aagaagtcaa aataggtatg gcgattgatg
     8941 atctccgtct tgaacgctgg caaaaagatc gagatatttt tgtgaaaaag gcagaatctc
     9001 tcggtgcgaa agtatttgta cagtctgcaa atggcaatga agaaacacaa atgtcgcaga
     9061 ttgaaaacat gattaaccgg ggtgtcgatg ttcttgtcat tattccgtat aacggtcagg
     9121 tattaagtaa cgttgtaaaa aaagccaaac aagaaggtat taaagtatta gcttacgacc
     9181 gtatgattaa cgatgcggat atcgattttt atatttcttt cgataacgaa aaagtcggtg
     9241 aactgcaggc aaaagccttg gtcgatattg ttccgcaagg taattacttc ctgatgggcg
     9301 gctcgccggt agataacaac gccaagctgt tccgcgccgg acaaatgaaa gtgttaaaac
     9361 cttacgttga ttctggaaaa attaaagtcg ttggtgacca atgggttgat ggctggttac
     9421 cggaaaacgc attgaaaatt atggaaaacg cgctaaccgc caataataac aaaattgatg
     9481 ctgtagttgc ctcaaacgat gccaccgcag gtggggcaat tcaggcatta agcgcgcaag
     9541 gtttatcagg gaaagtagca atttccggcc aggatgcgga tctcgcaggt attaaacgta
     9601 ttgctgccgg tacgcaaact atgacggtgt ataaacctat tacattgctg gcaaatactg
     9661 ccgcagaaat tgccgttgaa ttgggcaatg gtcaggagcc aaaagcagat acctcactga
     9721 ataatggcct gaaagatgtc ccctcccgcc tcctgacacc gatcgatgtg aataaaaaca
     9781 acatcaaaga tacggtaatt aaagacggat tccacaaaga gagcgagctg taagcgttac
     9841 gccccggcgc ggagcggggg cgtgatttct ctccatgccg cgtgaatgaa ttggcttagg
     9901 tggagtcgtt atgccttatc tacttgaaat gaagaacatt accaaaacct tcggcagtgt
     9961 gaaggcgatt gataacgtca gcttgcggtt gaatgctggc gaaatcgtct cactctgtgg
    10021 ggaaaatggg tctggtaaat caacgctgat gaaagtgctg tgtggtattt atccccatgg
    10081 ctcctacgaa ggcgaaatta tttttgcggg agaagagatt caggcgagtc acatccgcga
    10141 taccgaacgc aaaggtatcg ccatcattca ccaggaattg gccctggtga aagaattgac
    10201 cgtgctggaa aatatcttcc tgggtaacga aataacccac aatggcatta tggattatga
    10261 cctgatgacg ctacgctatc agaagctgct cgcacaggtc agtttatcca tttcacctga
    10321 tacccgcgtt ggcgatttag ggcttgggca acaacaactg gttgaaattg ccaaggcact
    10381 taataaacag gtgcgcttgt taattctcga tgaaccgaca gcctcattaa ctgagcagga
    10441 aacgtcggtt ttactggata ttattcgcga tctacaacag cacggtatcg cctgtattta
    10501 tatttcgcac aaactcaacg aagtcaaagc gatttccgat acgatttgcg ttattcgcga
    10561 cggacagcac attggtacgc gtgatgctgc cggaatgagt gaagacgata ttatcaccat
    10621 gatggtcgag cgagagttaa ccgcgattta ccctaatgaa ccacatacca ccggagatga
    10681 aatattacgt attgaacatc tgacggcatg gcatccggtc aatcgtcata ttaaacgagt
    10741 taatgatgtc tcgttttccc tgaaacgtgg cgaaatactg ggtattgccg gactcgttgg
    10801 tgccggacgt accgagacca ttcagtgcct gtttggcgtg tggcccggac aatgggaagg
    10861 aaaaatttat attgatggca aacaggtaga tattcgtaac tgtcagcaag ccatcaccca
    10921 ggggattgcg atggtacccg aagacagaaa gcgcgacggc atcgttccgg taatggcggt
    10981 tggtaaaaat attaccctcg ccgcactcaa taaatttacc ggtggcatta gccagcttga
    11041 tgacgcggca gagcaaaaat gtattctgga atcaattcag caactcaaag ttaaaacgtc
    11101 gtcccccgac cttgctattg gacgtttgag cggcggcaat cagcaaaaag cgatcctcgc
    11161 ccgctgtctg ttacttaacc cgcgcatcct cattcttgat gaacctacca ggggtattga
    11221 tattggcgcg aaatatgaga tctacaaatt aattaaccaa ctcgtccagc agggtattgc
    11281 cgttattgtc atctcttccg aattacctga agtgctcggc cttagcgatc gtgtactggt
    11341 gatgcatgaa gggaaactaa aagccaacct gataaatcat aacctgactc aggagcaggt
    11401 gatggaagcc gcattgagga gcgaacatca tgtcgaaaag caatccgtct gaagtgaaat
    11461 tggccgtacc gacatccggt ggcttttctg ggctgaaatc actgaatttg caggtcttcg
    11521 tgatgattgc agctatcatc gcaatcatgc tgttctttac ctggaccacc gatggtgcct
    11581 acttaagcgc ccgtaacgtc tccaacctgt tacgccagac cgcgattacc ggcatcctcg
    11641 cggtaggaat ggtgttcgtc ataatttctg ctgaaatcga cctttccgtc ggctcaatga
    11701 tggggctatt aggtggcgtc gcggcgattt gtgacgtctg gttaggctgg cctttgccac
    11761 ttaccatcat tgtgacgttg gttctgggac tgcttctcgg tgcctggaac ggatggtggg
    11821 tcgcgtaccg caaagtccct tcatttattg tcaccctcgc gggcatgttg gcatttcgcg
    11881 gcatactcat tggcatcacc aacggcacga ctgtatcccc caccagcgcc gcgatgtcac
    11941 aaattgggca aagctatctc cccgccagta ccggcttcat cattggcgcg cttggcttaa
    12001 tggcttttgt tggttggcaa tggcgcggaa gaatgcgccg tcaggctttg ggtttacagt
    12061 ctccggcctc taccgcagta gtcggtcgcc aagctttaac cgctatcatc gtattaggcg
    12121 caatctggct gttgaatgat taccgtggcg ttcccactcc tgttctgctg ctgacgttgc
    12181 tgttactcgg cggaatgttt atggcaacgc ggacggcatt tggacgacgc atttatgcca
    12241 tcggcggcaa tctggaagca gcacgtctct ccgggattaa cgttgaacgc accaaacttg
    12301 ccgtgttcgc gattaacgga ttaatggtag ccatcgccgg attaatcctt agttctcgac
    12361 ttggcgctgg ttcaccttct gcgggaaata tcgccgaact ggacgcaatt gcagcatgcg
    12421 tgattggcgg caccagcctg gctggcggtg tgggaagcgt tgccggagca gtaatggggg
    12481 catttatcat ggcttcactg gataacggca tgagtatgat ggatgtaccg accttctggc
    12541 agtatatcgt taaaggtgcg attctgttgc tggcagtatg gatggactcc gcaaccaaac
    12601 gccgttcttg attttgataa aaattttctc aaagccggtt acgtattacc ggttttgagt
    12661 ttttgcatga ttcagcagga aaagaaccat gtttactaaa cgtcaccgca tcacattact
    12721 gttcaatgcc aataaagcct atgaccggca ggtagtagaa ggcgtagggg aatatttaca
    12781 ggcatcacaa tcagaatggg atattttcat tgaagaagat ttccgcgccc gcattgataa
    12841 aatcaaggac tggttaggag atgtcgtcat tgccgacttc gacgacaaac agatcgagca
    12901 agcgctggct gatgtcgacg tccccattgt tggggttggc ggctcgtatc accttgcaga
    12961 aagttaccca cccgttcatt acattgccac cgataactat gcgctggttg aaagcgcatt
    13021 tttgcattta aaagagaaag gcgttaaccg ctttgctttt tatggtcttc cggaatcaag
    13081 cggcaaacgt tgggccactg aacgcgaata tgcatttcgt cagcttgtcg ccgaagaaaa
    13141 gtatcgcgga gtggtttatc aggggttaga aaccgcgcca gagaactggc aacacgcgca
    13201 aaatcggctg gcagactgac tacaaacgct gccaccgcaa accgggatta ttgccgttac
    13261 tgacgcccga gcgcggcata ttctgcaagt atgtgaacat ctacatattc ccgtaccgga
    13321 aaaattatgc gtgattggca tcgataacga agaactgacc cgctatctgt cgcgtgtcgc
    13381 cctttcttcg gtcgctcagg gcgcgcggca aatgggctat caggcggcaa aactgttgca
    13441 tcgattatta gataaagaag aaatgccgct acagcgaatt ttggtcccac cagttcgcgt
    13501 cattgaacgg cgctcaacag attatcgctc gctgaccgat cccgccgtta ttcaggccat
    13561 gcattacatt cgtaatcacg cctgtaaagg gattaaagtg gatcaggtac tggatgcggt
    13621 cgggatctcg cgctccaatc ttgagaagcg ttttaaagaa gaggtgggtg aaaccatcca
    13681 tgcaatgatt catgccgaga agctggagaa agcgcgcagt ctgctgattt caaccacctt
    13741 gtcgatcaat gagatatcgc aaatgtgcgg ttatccatcg ctgcaatatt tctactctgt
    13801 ttttaaaaaa gcatatgaca caacgccaaa agagtatcgc gatgtaaata gcgaggtcat
    13861 gttgtagcga tgatgagaat tgtcggcgtc acatcaggga actcagcaca gccacctgat
    13921 gcgagcaaaa gccttatccg accgacaaat cagataaggc tcgaggaaat gaaattacat
    13981 atgcgccgcg attaaccgtt ggttatcctg gtacattgcg aacaggtagt tgttataact
    14041 cttccctttg gtcgagtagc ccttcagctt gtgaatcatc gctgtggcag tcacttcctg
    14101 atccgcttta cgcagctgtg cacgcgattt acggaacgaa gagtaagccg ggtgcgtatt
    14161 caggttagtg acataggcgc tcaccgattc tttgacagaa ctaaactgtg agtacccttt
    14221 cactttaccc ggcgcattgg tacaacgtcc tttcatgcat ttcatgccga acagattgtt
    14281 attgttgcgc gccagcttcg acgttcccca accgctttct gctgcagcca tcgtcgccac
    14341 catactggta gggataatgt ctacgcgttc aagcaaggta ttccacggga tttttcgcgt
    14401 attaccagac cacttcacct tgtagcgttt ggcgatgtct ttcagacgcg cacgctcagc
    14461 aggtgcccat tggccctgat actgttttga aatcagccag ttacgttctg cagtaatggc
    14521 tgcattttgg ctggtaatgt aaggcattac ggtccggaga aacgcctttt tccttggtgt
    14581 tccggaaggg tattttcgca aatcaggaag tgaactactc tttgcactat tgcgagaata
    14641 ctcttgttta ctgcttacct gtttattact agctttagtt aagtgggact tttgactcgc
    14701 tgttgttgtg tgcgtcttcg ctaacacctc actcgaaaac accagagtga gtaacataag
    14761 aatcatcgcc ccatatcgtc gtatgggagt caaaatcatc aggtctcctg gtcggattta
    14821 atcattccaa caccttatat ttttcacaaa tttgagagtt gaatctcaaa tcatatcaaa
    14881 aatagctgtc aagagcaccc caaggaatag tccaaatctg aaactatgtc acgagttaac
    14941 gattcagatt ggcgctaaat cgcagaaaat gtgggggtta tcgcaaaatt cagccgtttt
    15001 ttgcgcgcga tcgctcaccc ttgcttctca tcctgtggac ttaccgctca gggatgagtt
    15061 ttgtctggct tatcgctggc aaactgtctg aaatcgcagc aataaggact catccaccat
    15121 gaaactcgcc gcctgttttc tgacactcct tcctggcttc gccgttgccg ccagctggac
    15181 ttctccgggg ttccctgcct ttagcgaaca gggaacggga acatttgtca gccacgcgca
    15241 gttgcccaaa ggtacgcgtc cactcacgct aaattttgac caacagtgct ggcagcctgc
    15301 agatgcgata aaactcaatc agatgctttc cctgcaacct tgtagcaaca cgccgcctca
    15361 atggcgattg ttcagggacg gcaaatatac gctgcaaata gacacccgct ccggtacgcc
    15421 aacattgatg atttccatcc agaacgcgcc gaaccggtag caaacctggt ccgtgaatgc
    15481 ccgaaatggg atggattacc gctcacgctg gatgtcagcg ccactttccc ggaaggagcc
    15541 gccgtacggg attattacag ccaacaaatt gcgatagtga agaacggtca aataacgtta
    15601 caacccgctg ctaccagcaa cggtttactc ctgctggaac gggcagaaac tgacacatcc
    15661 gcccctttcg actggcataa cgccacggtt tactttgtgc tgacagatcg tttcgaaaac
    15721 ggcgatccca gtaatgacca gagttacgga cgtcataaag acggtatggc ggaaattggc
    15781 acttttcacg gcggcgattt acgcggcctg accaacaaac tggattacct ccagcagttg
    15841 ggcgttaatg ctttatggat aagcgcccca tttgagcaaa ttcacggctg ggtcggcggt
    15901 ggtacaaaag gcgatttccc gcattatgcc taccacggtt attacacaca ggactggacg
    15961 aatcttgatg ccaatatggg caacgaagcc gatctacgga cgctggttga tagcgcacat
    16021 cagcgcggta ttcgtattct ctttgatgtc gtgatgaacc acaccagcta tgccacgctg
    16081 gcggatatgc aggagtatca gtttggcgcg ttatatcttt ctggtgacga agtgaaaaaa
    16141 acgctgggtg aacgctggag cgactggaaa cctgccgccg gacaaacctg gcatagcttt
    16201 aacgattaca ttaatttcag cgacaaaaca ggctgggata aatggtgggg aaaaaactgg
    16261 atcagaacgg atatcggcga ttacgacaat cctggattcg acgatctcac tatgtcgctg
    16321 gcctttttgc cggatatcaa aaccgaatca actaccgctt ctggtctgcc ggtgttctat
    16381 aaaaacaaaa cggatactca cgctaaagtc atcgaaggct ttacacctcg cgattactta
    16441 acccactggt taagtcagtg ggtccgcgac tatgggattg atggttttcg ggtcgatacc
    16501 gccaaacatg ttgagttgcc cgcctggcag caactgaaaa ccgaagccag ctccgcgctt
    16561 cgcgaatgga aaaaaactaa ccccgacaaa gcattagatg acaaaccttt ctggatgacc
    16621 ggtgaagcct ggggccacgg cgtgatgcaa agtgactact atcgccacgg cttcgatgcg
    16681 atgatcaatt tcgattatca ggagcaggcg gcgaaagcag tcgattgtct ggcgcagatg
    16741 gatacgacct ggcagcaaat ggcggagaaa ttgcagggtt tcaacgtgtt gagctacctc
    16801 tcgtcgcatg atacccgtct gttccgtgaa gggggcgaca aagcagcaga gttattacta
    16861 ttagcgccag gcgcggtaca aatcttttat ggcgatgaat cctcgcgtcc gttcggtcct
    16921 acaggttctg atccgctgca aggtacacgt tcggatatga actggcagga tgttagcggt
    16981 aaatctgccg ccaacgtcgc gcactggcag aaaatcagcc agttccgcgc ccgccatccc
    17041 gcaattggcg cgggcaaaca aacgacactt tcgctgaagc agggctacgg ctttgttcgt
    17101 gagcatggcg acgataaagt gctggtcatc tgggctgggc aacagtgact tttccggctt
    17161 cccgttcgtc agtacctcgg gaagccgcca accaggataa aatgtcagcc ctaatcagcg
    17221 ttgcgggata aagcaccgct cactcttcaa cagaccgatt tgcaccccag caaatgtagc
    17281 gttattgtta ccttccttgc tacagagttc gacagatatc ccgctatgac attctccctt
    17341 tttggtgaca aatttacccg ccactccggc attacgctgc tgatggaaga tctgaacgac
    17401 ggtttacgca cgcctggcgc gattatgctc ggcggcggta atccggcgca gatcccggaa
    17461 atgcaggact acttccagac gctactgacc gacatgctgg aaagtggcaa agcgactgat
    17521 gcactgtgta actacgacgg tccacagggg aaaacggagc tactcacact gcttgccgga
    17581 atgctgcgcg agaagttggg ttgggatatc gaaccacaga atattgcact aacaaacggc
    17641 agccagagcg cgtttttcta cttatttaac ctgtttgccg gacgccgtgc cgatggtcgg
    17701 gtcaaaaaag tgctgttccc gctggcaccg gaatacattg gctacgctga cgccggactg
    17761 gaagaagatc tgtttgtctc tgcgcgtccg aatattgaac tgctgccgga aggccagttt
    17821 aaataccacg tcgattttga gcatctgcat attggcgaag aaaccgggat gatttgcgtc
    17881 tcccggccga cgaatccaac aggtaatgtg attactgacg aagagttgct gaagcttgac
    17941 gcgctggcga atcaacacgg cattccgctg gtgattgata acgcttatgg cgtcccgttc
    18001 ccgggtatca tcttcagtga agggcgcccg ctatggaatc cgaatatcgt actgtgcatg
    18061 agtctttcca agctggggct gcctggctcc cgctgcggca ttatcatcgc caatgaaaaa
    18121 atcatcactg ccatcaccaa tatgaacggc attatcagcc tggcacctgg cggtattggt
    18181 ccggcgatga tgtgtgaaat gattaagcgt aacgatctgc tgcgcctgtc tgaaacagtc
    18241 atcaaaccgt tttactacca gcgtgttcag gaaactatcg ccatcattcg ccgctattta
    18301 ccggaaaatc gctgcctgat tcataaaccg gaaggagcca ttttcctctg gctatggttt
    18361 aaggatttgc ccattacgac cgagcagctc tatcagcgcc tgaaagcacg cggcgtgctg
    18421 atggtgccgg ggcacaactt cttcccggga ctggataaac cgtggccgca tacgcatcaa
    18481 tgtatgcgca tgaactacgt accagagccg gagaaaattg aggcgggggt gaagattctg
    18541 gcggaagaga tagaaagagc ctgggctgaa agtcactaaa aattgcctga tgcgctacgc
    18601 ttatcaggcc tacatgattt ctgcaatata ttgaatttgg aagaatttgt aggccggata
    18661 aggcgtttac gccgcatctg gcataaacaa agcgcactat tccggcgttg agaaacgccg
    18721 gaaaacgttt caaacaggct gctgctgtag ccgatgccgc tgcactttct cgacatccat
    18781 gcactgcaac gcctttgtcg ggcaggcttc aacacaggcc ggccccgtct cccgatgcca
    18841 gcacagatcg cacttaatcg ccctcgcctt tttgcgcgaa gaaacgacct ccatcgcacc
    18901 aaacgggcaa gccagcatac agcttttaca gccaatgcaa cgtgtttgtt caacgaaaat
    18961 atgcccatgt tcgcggctta tcgcgtcaac agggcagaca ttcgcgcacg gtgcatcttc
    19021 acactgatga caggctactg gcgtggtcca gctgtggtct ttaatgacac gaatacggga
    19081 aataaactcg tctggtgaca acgcagcgca atcctgattc tcctgatgcg acactgcgca
    19141 agccacttca caggtacggc aaccgataca tttcgtcgca tccgcaataa taaaccggtt
    19201 catgatcatt cctttgcact aaaacagcag agtgccagag agcgcgtccc tactgctttg
    19261 atccaacgaa ggaaatgtta aagttttggc acttgtccaa agcgttatgt gatcgcgccc
    19321 aggtcatccc tgacagtaaa tcccagttca ttagaaatag cctgcgcggt ttcacgcagt
    19381 ggtttcagga gatttttctc tcccacctgt ttcagacgtg atgtcgaaag cgaaatcgac
    19441 acggcgtacg gcacccgccc atgaatatca aacaccggaa cagcaataca ggagacgccg
    19501 agttcgtttt cttctctgtc catcgccgct ccgctttcac gaatgtgcgc cagttcgtcg
    19561 aacatcgcgg gcagctcggt aatggtattg cgggttaacg gctggatctc atgctgatgg
    19621 ttttcccagt atgacttcac gtagtccggg tgaccaaacg ccatgtagat cttgcccatt
    19681 gcggaacagt agagcggcat atgctggcca atataggcac gggttcgcag catcccggtt
    19741 gtgggttcca gcttataaat caaaatagcg tgatcgtctt cgcggctgga gaagttaatg
    19801 gtttcaccag tggcgatgtt cagtgcctca agatgcggag cggcgatatg aatgatattc
    19861 agcgaagaca gcgccttctg cccgacggca ataaatttgg tagtcaggcg gtaactccct
    19921 gcggcgggcg cggtggtcac atagccgcag gactgtaatc cctgcaataa gcgatggacg
    19981 gtactcttat ttaaaccagc cagctccgaa agatgcgcca acggacaacc gtttggatag
    20041 ttgctcaaaa tctcaatcag catcaacccg cgaaacagac tctggcttcc ggctggacgc
    20101 tctttttcct gcgccatctc gttctctttt tttcccatca cttctttccc cattttgtcg
    20161 cgtcctgatg gtagcgcaaa gtgtgccgta gttcacgatc tcgacagata atttataacc
    20221 acttgatttt tatgtctttt gaaattcatc aatcagattg ctttgttaaa aagtgatcga
    20281 tatatttgaa atctagttcc gcatattgaa attttaagcc aaaaaagcga tcaaaaaaca
    20341 aggaagccta ggatgaaagt gacatttgag cagttaaaag cagcctttaa tcgggtctta
    20401 atttcacgcg gcgttgacag cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc
    20461 accgaatccg gcgtttattc tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg
    20521 gaaaacggcg atatcattcc tgatgcccaa cccaaacgta taaccagcct cggcgcaatt
    20581 gaacagtggg atgcccagcg ttcgatcggc aacctgacgg cgaaaaagat gatggatcgc
    20641 gccattgaac tggctgccga tcacggtatt ggtctggtgg cacttcgtaa tgccaaccac
    20701 tggatgcgcg gcggcagcta cggctggcag gcggcggaaa aaggctatat tggcatttgc
    20761 tggaccaact ccatcgccgt aatgccgccg tggggcgcaa aagagtgtcg catcggcact
    20821 aacccgctga tcgtcgccat tccttccacg ccgatcacca tggtcgatat gtcgatgttc
    20881 tcttacggca tgttagaagt taaccgcctg gcaggccgtc agctcccggt cgatggtggc
    20941 tttgatgatg agggcaattt gaccaaagaa cctggcgtta tcgagaagaa tcgccgcatt
    21001 ttgccgatgg gctactggaa aggttctggc atgtcgattg tgctggatat gatcgctact
    21061 ctcctttccg acggcgcatc ggttgccgaa gtcacccagg acaacagcga cgaatacggc
    21121 atttcacaaa tttttattgc cattgaagtg gacaagctta tcgacggtcc cacccgcgat
    21181 gccaagctgc aacgtatcat ggattacgtt actaccgctg aacgcgctga cgaaaaccag
    21241 gccatccgct tacccggcca cgaatttact accctgctgg ccgaaaaccg ccgtaacggc
    21301 atcactgttg atgacagcgt gtgggccaaa atccaggcgt tatgaggaga taagtcatga
    21361 tttttggaca tatcgcgcag cccaatccgt gccgtttgcc cgccgccatt gaaaaggcgc
    21421 tcgattttct gcgcgctacc aacttcaacg tcctggagcc gggcgttgtc gaaatcgacg
    21481 gcaagaatat ttatgcgca
//
LOCUS       DAZSNO010000055        21341 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZSNO010000055 DAZSNO010000000
VERSION     DAZSNO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21341
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.055"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..793
                     /locus_tag="ACHWN2_002030"
                     /pseudo
     CDS             1..793
                     /locus_tag="ACHWN2_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="ACHWN2_002031"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="ACHWN2_002031"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR8931570.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="ACHWN2_002032"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="ACHWN2_002032"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR8931571.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="ACHWN2_002033"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="ACHWN2_002033"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR8931572.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /gene="relA"
                     /locus_tag="ACHWN2_002034"
     CDS             complement(4991..7225)
                     /gene="relA"
                     /locus_tag="ACHWN2_002034"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR8931573.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="ACHWN2_002035"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="ACHWN2_002035"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR8931574.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="ACHWN2_002036"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="ACHWN2_002036"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR8931575.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="ACHWN2_002037"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="ACHWN2_002037"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR8931576.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="ACHWN2_002038"
     CDS             complement(12980..14320)
                     /locus_tag="ACHWN2_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR8931577.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="ACHWN2_002039"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="ACHWN2_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR8931578.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="ACHWN2_002040"
     CDS             complement(16108..16557)
                     /locus_tag="ACHWN2_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR8931579.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="ACHWN2_002041"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="ACHWN2_002041"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR8931580.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="ACHWN2_002042"
     CDS             complement(17357..17686)
                     /locus_tag="ACHWN2_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR8931581.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="ACHWN2_002043"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="ACHWN2_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR8931582.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="ACHWN2_002044"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="ACHWN2_002044"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR8931583.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..21245
                     /gene="ppnN"
                     /locus_tag="ACHWN2_002045"
     CDS             19881..21245
                     /gene="ppnN"
                     /locus_tag="ACHWN2_002045"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR8931584.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5249 a         5690 c         5091 g         5311 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctagt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc ataccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccaaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16861 cgtcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 tcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20521 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc actggtcaac gaattgatca
    20581 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20641 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20701 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20761 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20821 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20881 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    20941 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21001 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21061 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21121 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21181 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21241 cgtaatctcc cctcccccgg tgtaatgccg atcagttaag gatcagttga ccgatccagt
    21301 ggctgtgtaa gaatccggaa acgctcactt gtttccggat t
//
LOCUS       DAZSNO010000056        20779 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZSNO010000056 DAZSNO010000000
VERSION     DAZSNO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20779
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.056"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..579
                     /locus_tag="ACHWN2_002046"
     CDS             <1..579
                     /locus_tag="ACHWN2_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR8931585.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="ACHWN2_002047"
     CDS             597..1235
                     /locus_tag="ACHWN2_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8931586.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHWN2_002048"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHWN2_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR8931587.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHWN2_002049"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHWN2_002049"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR8931588.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="ACHWN2_002050"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="ACHWN2_002050"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR8931589.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHWN2_002051"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHWN2_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR8931590.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="ACHWN2_002052"
     CDS             complement(5552..6613)
                     /locus_tag="ACHWN2_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR8931591.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="ACHWN2_002053"
     CDS             complement(6610..8007)
                     /locus_tag="ACHWN2_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR8931592.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="ACHWN2_002054"
     CDS             8163..9161
                     /locus_tag="ACHWN2_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8931593.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHWN2_002055"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHWN2_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="ACHWN2_002056"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="ACHWN2_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHWN2_002057"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHWN2_002057"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR8931594.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHWN2_002058"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHWN2_002058"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR8931595.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15286)
                     /locus_tag="ACHWN2_002059"
                     /pseudo
     CDS             complement(14232..15286)
                     /locus_tag="ACHWN2_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(15296..16071)
                     /locus_tag="ACHWN2_002060"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="ACHWN2_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(16089..17141)
                     /locus_tag="ACHWN2_002061"
     CDS             complement(16089..17141)
                     /locus_tag="ACHWN2_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR8931596.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="ACHWN2_002062"
     CDS             complement(17172..18014)
                     /locus_tag="ACHWN2_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR8931597.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="ACHWN2_002063"
     CDS             complement(18001..18882)
                     /locus_tag="ACHWN2_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR8931598.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="ACHWN2_002064"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="ACHWN2_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(20200..>20779)
                     /gene="ycjM"
                     /locus_tag="ACHWN2_002065"
     CDS             complement(20200..>20779)
                     /gene="ycjM"
                     /locus_tag="ACHWN2_002065"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFR8931599.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5070 a         5668 c         5053 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttact
//
LOCUS       DAZSNO010000057        20712 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZSNO010000057 DAZSNO010000000
VERSION     DAZSNO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20712
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.057"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACHWN2_002066"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACHWN2_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR8931600.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACHWN2_002067"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACHWN2_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR8931601.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACHWN2_002068"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACHWN2_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR8931602.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACHWN2_002069"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACHWN2_002069"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR8931603.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="ACHWN2_002070"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="ACHWN2_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR8931604.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="ACHWN2_002071"
     CDS             complement(8217..9110)
                     /locus_tag="ACHWN2_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR8931605.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACHWN2_002072"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACHWN2_002072"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR8931606.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACHWN2_002073"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACHWN2_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR8931607.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACHWN2_002074"
     CDS             complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACHWN2_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR8931608.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACHWN2_002075"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACHWN2_002075"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR8931609.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACHWN2_002076"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACHWN2_002076"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR8931610.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="ACHWN2_002077"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="ACHWN2_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACHWN2_002078"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACHWN2_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR8931611.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACHWN2_002079"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACHWN2_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR8931612.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACHWN2_002080"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACHWN2_002080"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR8931613.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACHWN2_002081"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACHWN2_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR8931614.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4980 a         5727 c         5032 g         4973 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct ctgccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaatgt gc
//
LOCUS       DAZSNO010000058        20691 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZSNO010000058 DAZSNO010000000
VERSION     DAZSNO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.058"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            81..350
                     /locus_tag="ACHWN2_002082"
     CDS             81..350
                     /locus_tag="ACHWN2_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFR8931615.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="ACHWN2_002083"
     CDS             344..709
                     /locus_tag="ACHWN2_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR8931616.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHWN2_002084"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHWN2_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR8931617.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="ACHWN2_002085"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="ACHWN2_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR8931618.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHWN2_002086"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHWN2_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR8931619.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="ACHWN2_002087"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="ACHWN2_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR8931620.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHWN2_002088"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHWN2_002088"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR8931621.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHWN2_002089"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHWN2_002089"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR8931622.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEVPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="ACHWN2_002090"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="ACHWN2_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHWN2_002091"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHWN2_002091"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR8931623.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACHWN2_002092"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACHWN2_002092"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR8931624.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /gene="rtcR"
                     /locus_tag="ACHWN2_002093"
     CDS             14849..16447
                     /gene="rtcR"
                     /locus_tag="ACHWN2_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFR8931625.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="ACHWN2_002094"
     CDS             complement(16429..17187)
                     /locus_tag="ACHWN2_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR8931626.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACHWN2_002095"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACHWN2_002095"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR8931627.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACHWN2_002096"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACHWN2_002096"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR8931628.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="ACHWN2_002097"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="ACHWN2_002097"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR8931629.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20306..20587)
                     /locus_tag="ACHWN2_002098"
     CDS             complement(20306..20587)
                     /locus_tag="ACHWN2_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931630.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIS"
BASE COUNT         4893 a         5694 c         5213 g         4891 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgcat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct catggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aagtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggca cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctagt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DAZSNO010000059        20562 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZSNO010000059 DAZSNO010000000
VERSION     DAZSNO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20562
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.059"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="ACHWN2_002099"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="ACHWN2_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR8931631.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHWN2_002100"
     CDS             complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACHWN2_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HFR8931632.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHWN2_002101"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHWN2_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR8931633.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="ACHWN2_002102"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="ACHWN2_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="ACHWN2_002103"
     CDS             1631..2341
                     /locus_tag="ACHWN2_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR8931634.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHWN2_002104"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHWN2_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR8931635.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4129)
                     /locus_tag="ACHWN2_002105"
                     /pseudo
     CDS             complement(3469..4129)
                     /locus_tag="ACHWN2_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            4282..6615
                     /gene="bisC"
                     /locus_tag="ACHWN2_002106"
     CDS             4282..6615
                     /gene="bisC"
                     /locus_tag="ACHWN2_002106"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR8931636.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6584..7024)
                     /locus_tag="ACHWN2_002107"
     CDS             complement(6584..7024)
                     /locus_tag="ACHWN2_002107"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR8931637.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7021..7584)
                     /gene="tag"
                     /locus_tag="ACHWN2_002108"
     CDS             complement(7021..7584)
                     /gene="tag"
                     /locus_tag="ACHWN2_002108"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR8931638.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7742..8440
                     /locus_tag="ACHWN2_002109"
     CDS             7742..8440
                     /locus_tag="ACHWN2_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR8931639.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8669..9877
                     /locus_tag="ACHWN2_002110"
     CDS             8669..9877
                     /locus_tag="ACHWN2_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8931640.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10201..11892
                     /gene="eptB"
                     /locus_tag="ACHWN2_002111"
     CDS             10201..11892
                     /gene="eptB"
                     /locus_tag="ACHWN2_002111"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR8931641.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11984..12060
                     /locus_tag="ACHWN2_002112"
     tRNA            11984..12060
                     /locus_tag="ACHWN2_002112"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12018..12020,aa:Pro,seq:cgg)
     gene            12804..14411
                     /gene="dppA"
                     /locus_tag="ACHWN2_002113"
     CDS             12804..14411
                     /gene="dppA"
                     /locus_tag="ACHWN2_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR8931642.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14719..15738
                     /gene="dppB"
                     /locus_tag="ACHWN2_002114"
     CDS             14719..15738
                     /gene="dppB"
                     /locus_tag="ACHWN2_002114"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR8931643.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15748..16674
                     /gene="dppC"
                     /locus_tag="ACHWN2_002115"
     CDS             15748..16674
                     /gene="dppC"
                     /locus_tag="ACHWN2_002115"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR8931644.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALV
                     LVAIFGPSIGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFIN
                     IFPNCLAPLIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWV
                     VTFPGLAILLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16685..17668
                     /gene="dppD"
                     /locus_tag="ACHWN2_002116"
     CDS             16685..17668
                     /gene="dppD"
                     /locus_tag="ACHWN2_002116"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR8931645.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17665..18669
                     /gene="dppF"
                     /locus_tag="ACHWN2_002117"
     CDS             17665..18669
                     /gene="dppF"
                     /locus_tag="ACHWN2_002117"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR8931646.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18699..19970)
                     /locus_tag="ACHWN2_002118"
     CDS             complement(18699..19970)
                     /locus_tag="ACHWN2_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR8931647.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20446..20553
                     /locus_tag="ACHWN2_002119"
     CDS             20446..20553
                     /locus_tag="ACHWN2_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR8931648.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4924 a         5090 c         5409 g         5139 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggatctt
     4561 attcaccaac aacataaacg cattcgtgag gcttatggtc cggcatcgat ttttgctggt
     4621 tcctacggct ggcgttcaaa cggcgtgctg cataaggcct cgacattatt acaacgctat
     4681 atggcgctgg caggcggtta taccgggcat ctgggggatt attcgaccgg tgcggcacag
     4741 gcgatcatgc cgtatgtcgt aggtggcagc gaagtttatc aacagcaaac cagttggccg
     4801 ctggtgctgg aacatagcga tgtcgtggtg ttgtggagtg ctaacccact caatacgctg
     4861 aaaattgcgt ggaatgcatc cgatgagcag gggctttctt acttttccgc actgcgtgac
     4921 agtgggaaaa agctgatctg cattgatcca atgcgatcgg aaaccgtcga tttctttggc
     4981 gataaaatgg agtgggtggc accgcacatg ggcaccgatg ttgcgctgat gctggggatc
     5041 gcccatacgc tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg ttgcaccaca
     5101 ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg acggaatagc gaaaaacgcc
     5161 gaatgggcgg cagagatttg tggtgttggc gcagcgaaaa tccgcgagct ggcggctatt
     5221 ttccaccaaa ataccaccat gctgatggca ggctggggaa tgcagcgcca acagtttggt
     5281 gagcaaaaac actggatgat cgtcacgctg gcagcaatgt tggggcaaat cggcacaccc
     5341 ggcggcggtt ttggtctttc ttaccatttt gccaatggtg gtaaccccac gcggcgttct
     5401 gcggtgctct cttccatgca gggcagcttg ccgggtggct gcgatgcggt ggataaaatc
     5461 cctgttgccc gcattgttga agcactggaa aaccctggtg gcgcatatca acacaacggt
     5521 atgaaccgac atttcccgga tattcgtttt atctggtggg cgggcggtgc caactttact
     5581 catcatcagg ataccaatcg tctgatccgt gcctggcaaa aaccggagct ggtggtgatc
     5641 tctgaatgct tctggacggc ggcggcgaaa cacgcggata ttgttctgcc tgcgactacc
     5701 tcttttgagc gtaatgatct caccatgacc ggtgattaca gtaatcagca tctggtgccg
     5761 atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt ttttgccgag
     5821 ttgagtgaac gttgggagaa gggcggctat gcgcggttta cggaaggaaa aagtcagctg
     5881 caatggctgg aaacgtttta taacgttgcc cggcagcgcg gggcaagcca gcaggttgaa
     5941 ttgccgccat ttgctgagtt ctggcaagcc aaccagttaa ttgagatgcc ggaaaacccg
     6001 gacagcgagc ggtttattcg cttcgctgat ttttgccgcg atccgctggc gcatccgtta
     6061 aaaaccgcca gcggcaagat tgaaatcttc tcacagcgta ttgccgatta cggttacccg
     6121 gattgccctg ggcatccaat gtggctggag ccggacgaat ggcagggcaa tgccgaacca
     6181 gaacagttgc aggtactttc tgcccatccg gcgcaccgcc tgcacagcca gctgaattac
     6241 agttctctgc gcgaattgta cgcggtagca aaccgtgagc ctgtcaccat tcatcctgac
     6301 gatgctcagg cgcgcggcat aaccgaaggg gatatggtgc gggtgtggaa ctcccgtggg
     6361 cagatccttg ccggagcggt cattagcgag ggaattaaac ctggcgtgat ttgcattcac
     6421 gaaggggcat ggccggatct ggatttaacc gctgacggta tttgtaaaaa cggcgcagtg
     6481 aacgtgctga ccaaagatct ccccagctcg cggctgggga atggctgtgc gggtaatacg
     6541 acgctggcat ggctggaaaa atacaacggt ccggaactga cacttacagc gtttgaacca
     6601 ccggccagct cataatccag gtgggtagtt gggtttcatc ctgccatgcg caatcgacaa
     6661 tgtgaaaacc ctgcgcacgg taaaaatcta tcgccggttg atttttttga taaacctcca
     6721 gcatcagatg gggatagcgc tgctgcacat actgcatcag cgccttacca ataccgcgtc
     6781 tgacggcctt cggtgcgaca aacattgctg ccagaaatcg gccttccata atgctgacaa
     6841 aaccgagaag cttaccgtcc tcttcccaga cccagttttg cgcgttggca agataggcat
     6901 cccgcaccag cggaatgcag tcacgccagt aattcgcttt tataaaggga tgcccccagg
     6961 ttgtactttc cagccacagt tcgaggatcg cggggagttc tgaacgttgc gcttcccgaa
     7021 tcatggttta tttcccagat agcaacagca gccaaccaca tgatcattca ccagcccaca
     7081 tgcctgcata aaggagtaac agattgtggt gccgacaaac ttaaaaccac gttttttcag
     7141 tgccttagat agggcgtcgg aggcggacgt agatgtggga atttcgctca acgttgtggc
     7201 ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca aagtcgggaa acggttcgcc
     7261 gttctgttcc atttgcaggt acgcccgcgc attaccaata attgcctgaa ttttccctcg
     7321 atggcggata atcccggcgt cctgtaccag tctttcgaca tcctcttcct gcattgctgc
     7381 gaccttcacc ggatcgaact gatgaaagta ggcgcgatag ttttcgcgtt ttttgaggac
     7441 ggtgatccac gataacccag cctgctgccc ttcaaagcag atcatttcga acagtttttt
     7501 actgtcagtt tcaggcacgc cccactcatt atcatggtag gcaatataaa gcgggccctg
     7561 actcacccag ccgcaacgtt ccatactttc cctcgctatg tacccgatcg taaaaatttc
     7621 agccgctttt cttgacgcat cggtgaaaaa gtcaatattt aatacagggc tactatgccc
     7681 gatatccttc atacaatgac aataatattg ccgaattcgg ctcttccctg gagtcgtgtt
     7741 aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga gcgtggtaat
     7801 cagtgccttt tttctcaaca cagcttacgc ctggcaacaa gaatatatcg ttgatacgca
     7861 acccggacat tccacagagc gttacacctg ggatagtgat catcaacctg attacaacga
     7921 tattttgtcg caacgtattc aaagtagcca aagggcgctg ggactggaag tcaatctggc
     7981 ggaagaaact cctgtggatg tgaccagcag tatgagtatg ggctggaatt ttcctttata
     8041 tgaacaggtt acaaccggcc cggtcgcggc attacattac gatggcacaa ccacctcgat
     8101 gtataacgag tttggcgaca gtactaccac gctgaccgat ccgttatggc atgccagcgt
     8161 gagtagctta ggctggcgtg ttgactcccg gcttggcgat ctccgaccct gggcgcaaat
     8221 cagctataac cagcaatttg gcgagaatat ctggaaggcg caatcaggcc tgagccggat
     8281 gacggcgaca aaccagaacg gcaactggct ggatgtcacc gtaggcgctg atatgttgct
     8341 caatcaaaat attgccgcct atgctgcgct aactcaggca gaaaatacca ctaataatag
     8401 cgactatctg tatacgatgg gggttagcgc cagattttaa cgtaacagtc acaattgaaa
     8461 ccattgaata acaacagttg tggcgatagt gggtgctaac ttaccaaata ataaatttgg
     8521 tgaataattg tcgcgttatt cattcctgaa ctaaggcatt tcattccgtt ctgatggcat
     8581 ttcatgccgt ttttccccaa gcataaaatg cacttcgtta tggttgtcgg cagagatttt
     8641 tcctttttat tactgcagga atactgccat gacaccttca aattatcagc gtacccgctg
     8701 gctgacactc atcggtacta tcattaccca gtttgcgctg gggtcggttt atacccggag
     8761 cctgtttaat ggcgcgcttt ctgccaagct ggatgcgccg gtaagccagg tcgctttctc
     8821 tttcggcttg ttaagtctgg ggctggcaat ttcgtcttct gttgcgggca aattgcagga
     8881 acgttttggc gttaaacgcg tcaccgtggc ttccggcatt ttgctgggat taggcttctt
     8941 cctgaccgcg cattccaaca acctgatgat gctgtggtta agcgccggtg tgctggtcgg
     9001 tctggcggat ggcgcgggtt acctgctgac gctctctaac tgcgtgaagt ggttcccgga
     9061 gcgtaaaggt ctgatctccg cgttcgctat cggttcttat ggtctgggta gcctgggttt
     9121 caaatttatc gacacgcagc tgctcgaaac ggtgggtctg gaaaaaacct ttgtgatttg
     9181 gggagcgatt gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag acgcaccaaa
     9241 acaggaagtg aaaaccagca atggtgtggt ggagaaagat tacacgctgg cagagtcgat
     9301 gcgtaaaccg cagtactgga tgttagcggt aatgttcctg actgcctgca tgagcggcct
     9361 gtacgtgatt ggggtagcga aagatatcgc ccaaagtctg gcacaccttg atgtggtttc
     9421 cgcagccaat gcagtaacgg ttatttccat cgctaacctt tcaggtcgtc tggtgctggg
     9481 cattctgtct gacaaaattg cccgtatccg tgttatcacc attggtcagg tgatttcgct
     9541 ggtgggtatg gcggccctgc tgtttgcacc attaaatgca gtgacgttct ttgcagcgat
     9601 tgcctgcgtg gcatttaact ttggcggcac tattaccgtc tttccatcac tggtcagtga
     9661 gttcttcggc ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg gtttcggtat
     9721 cggtagcatt tttggttcga ttatcgcctc actgtttggc ggtttctatg tgaccttcta
     9781 cgtgattttc gccctgctga ttctgtcatt ggcgctttct acgacgattc gtcagccaga
     9841 gcagaaaatg ttgcgtgagg cgcatggctc cctttaatgc tcagccgcga gctactgggc
     9901 ttcagcttcc gtgtgcatca aacgcatcaa cgaaatgtat aaacaatgac tcgtaaaatt
     9961 tgggccagtt aactggccct ttttactgct ttttgtaacg ctaaagtctc ttttcaaact
    10021 tgcatttttg taaatttgtg cttcatgcac actctttccc cacacttttt cgctttgctg
    10081 tggtctactt attcgcgcgt gtagatttta cttatctgac tacctccgca cttttcccct
    10141 gccgggcctg aaaagccgct aagcagggtg ttatcacctg tttgtccagg gtttgtttgc
    10201 atgagataca tcaaatcgat tacacagcag aagctgagct ttttgcttgc aatctatatt
    10261 ggccttttta tgaatggcgc ggttttttat cgccgcttcg gcagctatgc gcacgatttt
    10321 accgtctgga aaggcgtttc tgctgttgtt gaactggccg ccaccgtact ggtgaccttc
    10381 tttttactac gtcttctatc gctgtttggc cgccgcagct ggcgtattct ggcgtcgctg
    10441 gtggtgctct tttccgcagg tgccagctat tacatgacct tccttaatgt ggtcattggt
    10501 tatggcatca tcgcttccgt catgaccacc gatatcgacc tgtcaaaaga agttgttggt
    10561 ctgaacttta ttctctggtt aatcgccgtt agtgcattgc ctcttatcct tatctggaat
    10621 aaccgctgtc gctacacctt gctccgacaa ctgcgaaccc cagggcagcg tattcgcagc
    10681 ctggtggtcg tcgtactggc gggtattatg gtttgggcac cgattcgttt gctggatatc
    10741 cagcagaaga aagtggagag ggcgaccggc gttgatttgc cgagttatgg cggtgtcgta
    10801 gcgaactctt atctgccatc aaactggctt tctgcgttgg ggctgtatgc ctgggcgcgg
    10861 gtcgatgaat cttccgataa taattcattg cttaatccgg cgaagaagtt tacataccag
    10921 gctccacaaa acgttgatga tacttatgtc gtgtttatca tcggtgaaac cacgcgttgg
    10981 gaccatatgg gtattttcgg ctatgagcgt aataccacgc cgaaactggc ccaggagaaa
    11041 aatctggcgg cgttccgtgg ttactcctgt gataccgcaa ccaaactctc actgcgttgc
    11101 atgtttgtac gtcagggggg cgcggaagat aatccgcagc gcacattaaa agaacagaac
    11161 attttcgcgg ttctgaagca gttaggattc agttctgacc tctacgctat gcagagcgaa
    11221 atgtggttct acagcaacac gatggcagac aacattgctt atcgtgagca gattggtgcg
    11281 gagccacgta atcgtggcaa gccggtagat gatatgttgc tggtagacga aatgcagcaa
    11341 tcgctagggc gcaacccgga tggtaagcat ctgatcattc tgcataccaa aggttcgcat
    11401 tttaactaca cccagcgtta tccgcgtagc ttcgcgcagt ggaagccgga atgtattggt
    11461 gttgatagcg gctgtaccaa agcgcagatg atcaactcct atgacaactc ggtgacctat
    11521 gtggatcact ttatctccag cgtgattgat caggttcgcg ataagaaagc gattgtgttc
    11581 tacgcagctg accacggtga gtcaattaat gaacgcgagc acctgcacgg cacgccgcgt
    11641 gaactggcac cgccggagca gttccgcgta ccgatgatgg tctggatgtc agataaatat
    11701 ctggaaaatc cggccaatgc gcaggcgttt gcgcagctga aaaaagaagc cgacatgaaa
    11761 gtgccacgcc gtcacgtaga gctgtacgac accatcatgg gttgtcttgg ctatacttca
    11821 ccggatggtg gaattaacga aaacaacaac tggtgtcaca tcccgcagac aaaagtggca
    11881 gcggctaact aagcggcctg ctgactttct cgccgatcaa aaggcatttt gctattaagg
    11941 gattgacgag ggcgtatctg cgcagtaaga tgcgccccgc attcggtgat tggcgcagcc
    12001 tggtagcgca cttcgttcgg gacgaagggg tcggaggttc gaatcctcta tcaccgacca
    12061 aattcgaaaa gcctgctcaa cgagcaggtt tttttgcatt tgtagctcat gaggatgaga
    12121 acctccgggg gcagggaggc tcgactcgac gtaggcctga taagacgcgc cagcgtcgca
    12181 tcaggcaacg gcaccgcact tattgtcgga tgcggcgcga ccgccttatt cgaccaacac
    12241 accacacatt ttcctgccaa ctttgcgccc catatctcac tatccattaa cggatttgtg
    12301 acagaaacca ttgtcttttt ttatatttgc ttaaatcttt tttgcgttac ttatggctgg
    12361 cgctggcatt ttccgctgtg cgttttagcg ttcatggtat taatcgctca gatatccacc
    12421 ttttcctcag atttttttca ttaaatgcat aaaacttaat caccaaatgt tgctaaatat
    12481 taagctgatt gttctggtta tgttggagta gcacaaattt ctctgctgca aatagctgtt
    12541 tgaagttttt ttaatctttg tttgtggatt ctcaccgttg gtgtaaatcc cggttggctg
    12601 tattgacgtt ttcacattct gttgacagat tgtaggtcac gaggggcatt ttatggagga
    12661 tccgcactgt tacactgatg ttaattagta cggcatcccc acctcataac gttgacccga
    12721 ccgggcaaaa aacaaaaaag gtcaggcagc gacaacccac tgcaaagggt taaaacaaca
    12781 aacatcacaa ttggagcaga ataatgcgta tttccttgaa aaagtcaggg atgctgaagc
    12841 ttggtctcag cctggtggct atgaccgtcg cagcaagtgt tcaggctaaa actctggttt
    12901 attgctcaga aggatctccg gaagggttta acccgcagct gtttacctcc ggcaccacct
    12961 atgacgcctc ttccgtcccg ctttataacc gcctggttga atttaaaatc ggcaccaccg
    13021 aagtgatccc gggcctcgct gaaaagtggg aagtcagcga agacggtaaa acctatacct
    13081 tccatctgcg taaaggtgtg aagtggcacg acaataaaga attcaaaccg acgcgcgaaa
    13141 tgaacgccga tgacgtggtg ttctcgttcg atcgtcagaa aaacgcgcaa aacccgtacc
    13201 ataaagtttc tggcggcagc tacgaatact tcgaaggtat gggcttgccg gagctgatca
    13261 gcgaagtgaa aaaggtggac gataacaccg ttcagtttgt gctgactcga ccggaagcgc
    13321 cgttcctcgc tgacctggca atggacttcg cctctattct gtcaaaagaa tatgctgacg
    13381 cgatgatgaa agccggtaca ccggaaaaac tggacctcaa cccaatcgga accggtccgt
    13441 tccagttaca gcagtaccaa aaagattccc gtatccgcta caaagcgttt gatggctact
    13501 ggggcaccaa accgcagatc gatacgctgg tcttctctat tacccctgat gcttccgtgc
    13561 gttacgcgaa attgcagaag aatgaatgcc aggtgatgcc gtacccgaac ccggcagaca
    13621 tcgctcgcat gaagcaggat aaatccatca acctgatgga aatgccgggg ctgaacgtcg
    13681 gctacctctc gtataacgtg cagaaaaaac cgctggatga cgtgaaagtt cgccaagcac
    13741 taacctacgc ggtgaacaaa gacgcgatca tcaaagcggt ttatcagggc gcgggtgtat
    13801 cagcgaaaaa cctgatcccg ccaaccatgt ggggctataa cgacgacgtt caggattaca
    13861 cctacgatcc tgaaaaagcg aaagccttgc tgaaagaagc gggtctggaa aaaggtttct
    13921 ccatcgacct gtgggcgatg ccggtacaac gtccgtataa cccgaacgct cgccgcatgg
    13981 cggagatgat tcaggcagac tgggcgaaag tgggcgtgca ggccaaaatc gtcacctacg
    14041 aatggggtga gtacctcaag cgtgcgaaag atggcgaaca tcagacggta atgatgggct
    14101 ggactggcga taacggggac ccggataact tcttcgccac cctgttcagc tgcgccgcct
    14161 ctgaacaagg ttctaactac tcaaaatggt gctacaaacc gtttgaagat ctgattcaac
    14221 cggcgcgtgc taccgacgac cataacaaac gcgttgaact gtacaagcaa gcgcaggtgg
    14281 tgatgcacga tcaggctccg gcactgatca tcgctcactc caccgtgttt gaaccggtac
    14341 gcaaagaagt caaaggctat gtggttgatc cattaggcaa acatcacttc gaaaacgtct
    14401 ctatcgaata attaaaagcc atacaagact gatggcaaag gcaaaactgc ctgatgtgct
    14461 ccgcttatca ggcctacgaa aattctgcaa tgtattgaat ttgcacgatt ttgtaggccg
    14521 gataaggcgt taacgccgca tccggcataa acaaagcgca ctttgtcaac aatctgtata
    14581 cccggtggcg ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attctctgtc
    14641 cgacaagggc gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgagtca
    14701 ctatagagaa tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc
    14761 acgtttatcg gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatccg
    14821 gtgatgatca tggcaggcga acgtgggatc tcccctgagc gtcacgtgca gctgctggct
    14881 gaactcggct tagataaacc gatgtggcag cagtatctcc attacatttg gggcgtaatg
    14941 cacggcgatc taggcatttc aatgaaaagc cgaattccag tatgggaaga gttcgtgccg
    15001 cgctttcagg ccacgctgga acttggcgtc tgcgcgatga tttttgctac ggcagtcggt
    15061 attccggttg gtgtgctggc tgcggttaaa cgcggttcca ttttcgatca cactgcagtt
    15121 ggcctggcgc tgaccggcta ctcgatgccg atcttctggt ggggcatgat gctgatcatg
    15181 ctggtttcgg tgcactggaa cctgacgccc gtctccggtc gcgtgagcga tatggtgttc
    15241 ctcgatgact ccaatccgtt aaccggtttt atgctaatcg acaccgccat ctggggtgaa
    15301 gacggcaact ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt gctgggtact
    15361 attccgctag cggtcattgt gcgtatgaca cgctcctcga tgctggaagt gctgggcgag
    15421 gattacatcc gcaccgcgcg cgccaaaggg ctaacccgca tgcgggtgat tatcgtccat
    15481 gcgctgcgta acgcgatgct gccggtagtg accgttatcg gcctccaggt gggaacattg
    15541 ctggcggggg cgattctgac cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg
    15601 attgacgcac tgcaacgccg cgactatccg gtagtgcagg gcggcgtatt gctggtggcg
    15661 acgatgatta tcctcgtcaa cttgctggtc gatctgctgt acggcgtggt gaacccgcgt
    15721 attcgtcata agaagtaagg ggacatcatg tcacaggtta ctgaaaataa agtgattagc
    15781 gcaccggtgc cgatgacccc gttacaggag ttctggcact attttaaacg caacaaaggc
    15841 gcggtcgtcg ggctggttta cgtcgccatc gtgctgttca tcgcgatctt tgccaactgg
    15901 attgcgcctt ataacccggc ggatcagttc cgcgatgcgc tgctcgcccc gccagcctgg
    15961 caggaaggcg gcagtatggc acacttgctg ggcaccgatg acgtcggtcg tgatgtgctg
    16021 tcgcggctga tgtacggcgc gcgtttatcg ctgctggttg gctgtctggt ggtggtgttg
    16081 tcgctgatta tgggcgttat tctcggcctg atcgccggtt acttcggcgg cctgatcgcc
    16141 ggttacttcg gcggcctggt tgataacatt atcatgcgcg tggtcgatat catgctggcg
    16201 ctgccaagtc tgctgttggc actggtgctg gtggcgattt tcggcccgtc gattggtaac
    16261 gccgcgctgg cgctgacctt cgttgccttg ccgcactatg tgcgcttaac ccgcgccgcc
    16321 gtgctggtgg aggtgaatcg cgattacgtc accgcctctc gcgtggcagg tgccggggcg
    16381 atgcgccaga tgtttattaa catcttcccg aactgccttg cgccgctgat tgttcaggcg
    16441 tcgctcggtt tctctaacgc cattctcgat atggctgctc ttggtttcct cggcatgggg
    16501 gcacagccgc caacgcctga gtggggcacc atgctctccg acgtgttgca gttcgcgcaa
    16561 agcgcctggt gggtcgtgac cttccctggt ctggcgatcc tgttgacggt gctggcattt
    16621 aacctgatgg gtgacggtct gcgtgacgcg ctcgatccca aactaaagca gtaagaggtt
    16681 cgagatggcg ttattaaatg tagataaatt atcggtgcat ttcggcgacg aaagcgcacc
    16741 gttccgcgcc gtagaccgca tcagctacag cgtaaaacag ggcgaagtgg tcgggattgt
    16801 gggtgagtcc ggctccggta agtcggtcag ttcactggcg attatggggc tgattgatta
    16861 tccgggccgc gtaatggcag aaaaactgga gtttaacggc caggatttgc agcgtatctc
    16921 agaaaaagag cgccgcaacc tggtgggtgc cgaagtggcg atgatcttcc aggacccgat
    16981 gaccagcctt aacccgtgct acaccgtggg tttccagatt atggaagcga ttaaagtgca
    17041 tcagggcggc aacaaaagta cccgccgtca gcgggcgatt gacctgctga atcaggtcgg
    17101 tattcccgat ccggcttcgc gtctggatgt ttacccgcat cagctttccg gcggcatgag
    17161 ccagcgcgtg atgatcgcca tggcgattgc ctgtcggcca aaactgctga ttgccgatga
    17221 accgaccacc gcgctggacg tgaccattca ggcgcaaatc atcgaactac tgctggagct
    17281 acagcagaaa gagaacatgg cgctggtgtt aattacccat gacctggcgc tggtggcgga
    17341 agctgcgcat aaaatcattg tgatgtatgc aggtcaggta gtagaaacgg gcgatgcaca
    17401 cgccatcttc catgcgccgc gtcacccgta tactcaggca ttgctgcgtg cgctgccaga
    17461 atttgctcag gacaaagaac gtctggcgtc gttgccaggt gttgttcccg gcaagtatga
    17521 ccgcccgaac ggctgcctgc ttaacccgcg ctgcccctat gccactgaca gatgtcgcgc
    17581 tgaagaaccg gcgctgaata tgctcgctga tgggcgtcag tccaaatgcc attacccact
    17641 tgatgatgcc gggaggccca cactatgagt acgcaagagg ccacctcgca acaaccgctg
    17701 ttgcaggcta tcgacctgaa aaaacattat ccggtgaaga aaggcatgtt cgtgccggaa
    17761 cgtctggtga aggcgctgga cggcgtttcg tttaaccttg aacgtggcaa aacgctggca
    17821 gtagtgggcg aatctggctg cggtaaatcg accctcggtc ggttgctgac gatgatcgaa
    17881 acgcccaccg gcggcgagct gtattatcag gggcaggatc tgctcaagca cgatccgcag
    17941 gcgcagaagc tgcgtcggca gaaaatccag atcgtcttcc agaatcccta cggttcgctg
    18001 aatccgcgta agaaagttgg gcaaattctc gaagagccgc tgcttattaa caccagctta
    18061 agcaaagaac agcgtcggga aaaagccctg tcgatgatgg caaaagtcgg cctgaaaacc
    18121 gagcactatg accgctatcc gcacatgttt tccggcggac aacgtcagcg tatcgctatt
    18181 gcccgtggtc tgatgctcga cccggatgtg gtgattgccg atgagccggt ttctgcgctg
    18241 gacgtctcag tgcgtgcgca ggtgctgaat ctgatgatgg atttgcagca ggagttgggg
    18301 ctttcttatg tctttatctc ccacgacctg tcggttgtgg agcacattgc tgatgaagtg
    18361 atggtgatgt acctaggccg ctgcgtggag aaaggaacga aggaccaaat cttcaataac
    18421 ccgcgccatc cgtacaccca ggcgctgctc tccgcgacgc cacgcctgaa cccggacgat
    18481 cgtcgtgaac gcatcaaact caccggtgaa ctgccaagcc cgctgaatcc tccgccgggt
    18541 tgcgccttca acgcccgctg tcgtcggcgc ttcggcccct gcacccagtt gcagccgcag
    18601 ctaaaagact acggaggtca actggtagct tgttttgctg ttgatcagga tgaaaatccg
    18661 cagcgttaac tcacaaaacc ggggccatgc gctccggttt actcaatcag cttcaacagt
    18721 ggcgacaagc atagcaaaat gccgtaaagc agaatcagcc aggctttaaa tccgcgaagt
    18781 ttttccagtt gtgcgacttt atagatcagg aaaaacggaa tgaggcacga cacaatgcca
    18841 tataacgggc ttcccaactg aaagaacacc agcactgata cacgaaacga aacccaaatc
    18901 gtcaacgtaa tgacgataaa agcgcagatc gccagagtca gcatgcgtga gttaattttc
    18961 ttggtatcaa taattcggct taacagattg agaataatgc ctttaatagc ctcgtggaaa
    19021 ccgagataaa tgccaaagaa tgcggtcagt acggcaaaga tattcagcac cgtagaggtg
    19081 atatgaatga tatgtccagg gatcacctgc gcggccagtg ccagtgccga gatattttgt
    19141 tcaaaggcag aaacggcttc ttcgtggcta attgagaagg taaacgaaaa ggcaaaaaac
    19201 aggatcaccg cgatgagcgt gatataacta attcggtggg tacgcagcgc gagccgggtt
    19261 gccagtactt tatctgcttc ccgtttacga taggcaatat tcattgggtt aagtacctga
    19321 ataaataccg cagaaaagaa gcaaaatgga atggtaagca ggacatcgcg gaaaaagacg
    19381 gaggcttgcg ggaaggcggt tatattggcg aaattccagt gcgggatcat cgcaaaaccg
    19441 aacacgacaa taatccctac tttgaccacc accattggcc cagaaatctt aaacagtaat
    19501 cgttcaccac cagacgcaat cgctatcagt acggcgaaaa tagcgacttt ataaagtaga
    19561 gattgtgaaa gatcggcatc ggttaaaccg aaggttttca ggtacgaggc gctgtcgaaa
    19621 accacggaga gagagtagat aaaaatcccg tggataatca tcaaaaagta gataaccccg
    19681 aggaaaattc cccagttctt ccccaggtaa tgactgataa tatcggtgta gtcattacag
    19741 gaatcgcttt cagaaagggt ttttaaataa atgtcctgca ctacccaggt gacaggataa
    19801 gcaatgatcg ctgcggtaat aaatacccaa attcccttca agccaatttg tactggcatc
    19861 agcacggttc cggcaccaat cgccatgcct atgcataata aaacccagcc gaagtcgtag
    19921 cgtgtaaacg gcaatttctg attgtgtttc gatagtgtgt tgtgctgcat aaagtcctgt
    19981 ccagaaagaa gtcggcctcg caaaaatgaa taaacccagt ttctcttgcg caacaggtgg
    20041 agaagtcgaa tacgcagtat gtaaaaaatg aggcaacaga ttcactgggg aggtcgcgag
    20101 gaggcgcaat tctacaaaat tgcgcatttc actaccagtc tggagtcagc ccggatatga
    20161 actgcaacta ttgcaaataa agaaattaag ctggaaggca tcgcgcaatg aggaagattg
    20221 ccgggtgctg acagtaaaaa gcaatcgtag aatcagcgtc aagccggaag gattccggtg
    20281 aggcgcaatg ttgcgggggc ttaatccctg gtggcattgg ttgctggaga gagaaaaccc
    20341 ccgcacgttg caggtatgta cctgacaaca taacgggggc taatcttgaa cctagaccac
    20401 tcaagaatag ccgcgaaacg ttgtcattac aacacaggcg gctatatgac gctcgcagag
    20461 ctgggcatgg ccttctggca tgatttagcg gctccggtca ttgctggcat tcttgccagt
    20521 atgatcgtga actggctgaa caagcggaag tagcgtgtca tg
//
LOCUS       DAZSNO010000060        20521 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZSNO010000060 DAZSNO010000000
VERSION     DAZSNO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.060"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="ACHWN2_002120"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="ACHWN2_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR8931649.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="ACHWN2_002121"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="ACHWN2_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFR8931650.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="ACHWN2_002122"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="ACHWN2_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR8931651.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACHWN2_002123"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACHWN2_002123"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR8931652.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACHWN2_002124"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACHWN2_002124"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR8931653.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="ACHWN2_002125"
     CDS             4449..4814
                     /locus_tag="ACHWN2_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR8931654.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="ACHWN2_002126"
     CDS             4964..5482
                     /locus_tag="ACHWN2_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR8931655.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="ACHWN2_002127"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="ACHWN2_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="ACHWN2_002128"
     CDS             6713..7195
                     /locus_tag="ACHWN2_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR8931656.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="ACHWN2_002129"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="ACHWN2_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR8931657.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="ACHWN2_002130"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="ACHWN2_002130"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR8931658.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="ACHWN2_002131"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="ACHWN2_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR8931659.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="ACHWN2_002132"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="ACHWN2_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR8931660.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="ACHWN2_002133"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="ACHWN2_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR8931661.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="ACHWN2_002134"
     CDS             10939..11730
                     /locus_tag="ACHWN2_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR8931662.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="ACHWN2_002135"
     CDS             11751..13037
                     /locus_tag="ACHWN2_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR8931663.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="ACHWN2_002136"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="ACHWN2_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR8931664.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="ACHWN2_002137"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="ACHWN2_002137"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR8931665.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="ACHWN2_002138"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="ACHWN2_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR8931666.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="ACHWN2_002139"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="ACHWN2_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR8931667.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="ACHWN2_002140"
     CDS             complement(16985..17275)
                     /locus_tag="ACHWN2_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR8931668.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="ACHWN2_002141"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="ACHWN2_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR8931669.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="ACHWN2_002142"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="ACHWN2_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR8931670.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="ACHWN2_002143"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="ACHWN2_002143"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="ACHWN2_002144"
     CDS             19137..20378
                     /locus_tag="ACHWN2_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8931671.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQLMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5357 c         4815 g         5049 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acaccccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attgcaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccaa gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgttgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtatttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagct tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DAZSNO010000061        20288 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZSNO010000061 DAZSNO010000000
VERSION     DAZSNO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20288
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.061"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="ACHWN2_002145"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="ACHWN2_002145"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR8931672.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="ACHWN2_002146"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="ACHWN2_002146"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR8931673.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3000)
                     /locus_tag="ACHWN2_002147"
     CDS             complement(1729..3000)
                     /locus_tag="ACHWN2_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8931674.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLSEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3183..4388)
                     /locus_tag="ACHWN2_002148"
     CDS             complement(3183..4388)
                     /locus_tag="ACHWN2_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8931675.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="ACHWN2_002149"
     CDS             complement(4488..4844)
                     /locus_tag="ACHWN2_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR8931676.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="ACHWN2_002150"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHWN2_002150"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="ACHWN2_002151"
     CDS             complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="ACHWN2_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR8931677.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6774..9830
                     /locus_tag="ACHWN2_002152"
     CDS             6774..9830
                     /locus_tag="ACHWN2_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR8931678.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9827..10237
                     /gene="menI"
                     /locus_tag="ACHWN2_002153"
     CDS             9827..10237
                     /gene="menI"
                     /locus_tag="ACHWN2_002153"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR8931679.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10337..10525
                     /locus_tag="ACHWN2_002154"
     CDS             10337..10525
                     /locus_tag="ACHWN2_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR8931680.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11073..11441
                     /gene="sufA"
                     /locus_tag="ACHWN2_002155"
     CDS             11073..11441
                     /gene="sufA"
                     /locus_tag="ACHWN2_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR8931681.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11450..12937
                     /gene="sufB"
                     /locus_tag="ACHWN2_002156"
     CDS             11450..12937
                     /gene="sufB"
                     /locus_tag="ACHWN2_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR8931682.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12947..13693
                     /gene="sufC"
                     /locus_tag="ACHWN2_002157"
     CDS             12947..13693
                     /gene="sufC"
                     /locus_tag="ACHWN2_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR8931683.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13668..14939
                     /gene="sufD"
                     /locus_tag="ACHWN2_002158"
     CDS             13668..14939
                     /gene="sufD"
                     /locus_tag="ACHWN2_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR8931684.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14936..16156
                     /gene="sufS"
                     /locus_tag="ACHWN2_002159"
     CDS             14936..16156
                     /gene="sufS"
                     /locus_tag="ACHWN2_002159"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR8931685.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16169..16585
                     /gene="sufE"
                     /locus_tag="ACHWN2_002160"
     CDS             16169..16585
                     /gene="sufE"
                     /locus_tag="ACHWN2_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR8931686.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16734..17738
                     /gene="ldtE"
                     /locus_tag="ACHWN2_002161"
     CDS             16734..17738
                     /gene="ldtE"
                     /locus_tag="ACHWN2_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR8931687.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACHWN2_002162"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACHWN2_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR8931688.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="ACHWN2_002163"
     CDS             complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="ACHWN2_002163"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR8931689.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(19768..19962)
                     /locus_tag="ACHWN2_002164"
     CDS             complement(19768..19962)
                     /locus_tag="ACHWN2_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931690.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT         5317 a         4861 c         5177 g         4933 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccaacca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcctgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtatccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaaagg tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgacag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgtactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
     5221 atatcaatta gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat
     5281 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg
     5341 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     5401 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga
     5461 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     5521 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga
     5581 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     5641 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     5701 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     5761 gtgccgccag cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     5821 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     5881 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     5941 tggtgccaat ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt
     6001 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     6061 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     6121 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     6181 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     6241 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     6301 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     6361 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     6421 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     6481 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     6541 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     6601 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     6661 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
     6721 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
     6781 cacagatttc cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
     6841 agcaacaagg ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga
     6901 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
     6961 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
     7021 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
     7081 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
     7141 aggccggggt gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg
     7201 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
     7261 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
     7321 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc
     7381 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
     7441 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
     7501 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
     7561 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
     7621 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
     7681 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
     7741 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat atgcctgaca
     7801 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
     7861 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg
     7921 tgattggctg gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca
     7981 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
     8041 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
     8101 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
     8161 tccgtccggc actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
     8221 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
     8281 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
     8341 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
     8401 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
     8461 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
     8521 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
     8581 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
     8641 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
     8701 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
     8761 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt
     8821 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
     8881 gttatttacg cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga
     8941 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
     9001 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac
     9061 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag
     9121 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
     9181 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
     9241 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
     9301 cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg
     9361 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
     9421 aacgtggcga gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac
     9481 atccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta
     9541 ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
     9601 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
     9661 accatgaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
     9721 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
     9781 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
     9841 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
     9901 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
     9961 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    10021 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    10081 caatgctaat cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt
    10141 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    10201 gtgctgttcg tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac
    10261 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    10321 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    10381 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    10441 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    10501 tgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    10561 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    10621 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    10681 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    10741 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
    10801 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    10861 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    10921 tgcctgttaa cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt
    10981 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
    11041 atacgctgaa agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc
    11101 cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg
    11161 agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct
    11221 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    11281 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca
    11341 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    11401 cccagaatga atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa
    11461 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaact ataaagaagg
    11521 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    11581 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    11641 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    11701 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    11761 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    11821 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    11881 cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat
    11941 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    12001 cggcaccgtg gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc
    12061 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    12121 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga
    12181 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    12241 gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    12301 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    12361 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
    12421 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    12481 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    12541 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    12601 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    12661 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    12721 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    12781 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    12841 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    12901 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    12961 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
    13021 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    13081 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    13141 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    13201 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    13261 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    13321 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    13381 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    13441 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    13501 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    13561 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
    13621 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    13681 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    13741 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    13801 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca
    13861 ttgcgggaga tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc
    13921 ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg
    13981 gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag
    14041 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    14101 gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag
    14161 aggtgaacac tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg
    14221 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta
    14281 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    14341 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    14401 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    14461 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    14521 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    14581 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    14641 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    14701 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    14761 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    14821 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    14881 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    14941 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    15001 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    15061 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag
    15121 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    15181 ccgttcggcg gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc
    15241 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    15301 gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    15361 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    15421 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    15481 actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    15541 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    15601 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    15661 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    15721 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    15781 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    15841 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    15901 tctcactctc tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    15961 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    16021 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    16081 ggccatgtat aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca
    16141 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    16201 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    16261 ccagcgtctg ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg
    16321 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    16381 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    16441 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    16501 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    16561 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    16621 tgccggatgg tcaatgttta cactgaccgg cacaggagtg ttttggtttc aggtgaacat
    16681 aagcaccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    16741 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    16801 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    16861 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa
    16921 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    16981 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    17041 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    17101 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    17161 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    17221 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    17281 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    17341 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    17401 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    17461 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    17521 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    17581 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    17641 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    17701 aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt gattttttat tgcttctggt tatcgattaa ataaaaaaag
    18301 cgcccatcag ggcgcttcga tatacaaatt aattcacaaa agcaatatta caggacgtga
    18361 acagatgcgg tgttagtagt gccgctcggt accagtgcac cagaaaccat aactacgacg
    18421 tcacctttgt gtgccagacc gctctgcaga gccagttctt tacccagacg gtagaaatca
    18481 tcagtagaag tgatctcttt aaccagctgc ggcacaacgc ctttgctcag taccaactga
    18541 tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg catccgggaa gtatttacgt
    18601 actgcgcgag cagatttacc gccctgggta gcaaccacga tcagcggagc atccagtttt
    18661 tcagcagttt caacggcacc acggcatacc gcttcggtga tgcgcagttt acggttgtca
    18721 ttgttgaact cgagacggct gttcatcacg cggtcggtac gttcgcagat ggtcgccatg
    18781 atagaaaccg cttccagcgg gtatttacct tttgcggatt caccagacag catcactgcg
    18841 tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg
    18901 tttttgatca tggaatccag catctgggtc gcagtgataa cgactttacg tgcacggata
    18961 catttttcga tcatcatctt ctgggcgaag ataacttctt ctaccgggat ttctacaccc
    19021 aggtcgccac gcgcaaccat gatgccgtca gaggcttcga ggatttcgtc gaagttgttg
    19081 aggccttcct ggttttcgat tttggagatg atgtggatgt tttcgccgcc gtgcgctttc
    19141 aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa taaaggaagc agcaacaaag
    19201 tctacgcctt tttcgcaacc aaagatcagg tcctgtttgt ctttttcagc cagtgctggc
    19261 agagcaatgg aaacgccagg caggttcaca cctttgtttt cgcccaggtc accgttgttc
    19321 agcactttac agataacttt gttaccttca atggcggtaa cttccatacc gatcagacca
    19381 tcgtcaacca gtacggtgtt gccaacagac aggtcagtag tgaaaccttc atacgttacc
    19441 gcaaccattt cgctgttgcc gataacagat ttatcagtgg tgaaagtaaa ggtctgacca
    19501 gctttcagag aaacatcgtt accgccttcc agtttcatgg tgcggatttc cggacctttg
    19561 gtatcaagca ggatagcggc ggttttacca gttttgctca tcacgttgcg cagattctga
    19621 atgcgctgac cgtgttctgc atagtcacca tgagagaagt tcagacgcat aacgttcatg
    19681 ccagcgtcca gcattttagc taacatctct tcagattcgg ttttcggtcc gatggtgcaa
    19741 acaattttgg tctttttcat gacagtctta gtctttaagt tgagaaggat gggagaaact
    19801 tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta cgaaagtggt ggtgcctcgc
    19861 tctaaggata ggtgacatcg aaaaagcgtg cagaggaatg tgtgcttgtg tctcagccca
    19921 acgtgagtgt ctgtttttga gtcgttgagt tctacagtcc aaagtgctga aaccattcaa
    19981 gagtcaattg gcgcgcatta tacgctgaaa ttaatccaaa aggaaaacaa aaacagcgtt
    20041 tcaaaaaata tacaacttgt tagacaataa attgttatca aagatttaac gttccaggga
    20101 aaaggttacg caaaaacttc gctgtttgcg caacgtctgg tgccgaagtt gtcagtgtga
    20161 ggataagtga aagttcatcc ggtgtataaa tatatcatcg ctcaattgct acagttaaaa
    20221 taatttatcg cgttatttgt ctgattttgt ttgatttttg tttaattggt tagattgata
    20281 tatgctga
//
LOCUS       DAZSNO010000062        19702 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZSNO010000062 DAZSNO010000000
VERSION     DAZSNO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.062"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHWN2_002165"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHWN2_002165"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR8931691.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHWN2_002166"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHWN2_002166"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR8931692.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHWN2_002167"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHWN2_002167"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR8931693.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="ACHWN2_002168"
     CDS             6038..6658
                     /locus_tag="ACHWN2_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR8931694.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="ACHWN2_002169"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="ACHWN2_002169"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR8931695.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHWN2_002170"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHWN2_002170"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR8931696.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHWN2_002171"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHWN2_002171"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR8931697.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="ACHWN2_002172"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="ACHWN2_002172"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR8931698.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10068..11069)
                     /locus_tag="ACHWN2_002173"
     CDS             complement(10068..11069)
                     /locus_tag="ACHWN2_002173"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8931699.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     ELLYRSMKFVSARNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHWN2_002174"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHWN2_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHWN2_002175"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHWN2_002175"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR8931700.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHWN2_002176"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHWN2_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR8931701.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHWN2_002177"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHWN2_002177"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR8931702.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHWN2_002178"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHWN2_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR8931703.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHWN2_002179"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHWN2_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR8931704.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHWN2_002180"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHWN2_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR8931705.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4682 a         5507 c         5034 g         4479 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttttc gg
//
LOCUS       DAZSNO010000063        19540 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZSNO010000063 DAZSNO010000000
VERSION     DAZSNO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19540
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.063"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..356
                     /locus_tag="ACHWN2_002181"
     CDS             <1..356
                     /locus_tag="ACHWN2_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR8931706.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="ACHWN2_002182"
     CDS             complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="ACHWN2_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR8931707.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="ACHWN2_002183"
     CDS             complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="ACHWN2_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR8931708.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3111..3263
                     /gene="mokC"
                     /locus_tag="ACHWN2_002184"
     CDS             3111..3263
                     /gene="mokC"
                     /locus_tag="ACHWN2_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR8931709.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="ACHWN2_002185"
     CDS             complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="ACHWN2_002185"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR8931710.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="ACHWN2_002186"
     CDS             complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="ACHWN2_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR8931711.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6879..7283
                     /locus_tag="ACHWN2_002187"
     CDS             6879..7283
                     /locus_tag="ACHWN2_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR8931712.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7309..8022
                     /gene="msyB"
                     /locus_tag="ACHWN2_002188"
     CDS             7309..8022
                     /gene="msyB"
                     /locus_tag="ACHWN2_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR8931713.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8171..8737
                     /gene="satP"
                     /locus_tag="ACHWN2_002189"
     CDS             8171..8737
                     /gene="satP"
                     /locus_tag="ACHWN2_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR8931714.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8772..9359)
                     /gene="mog"
                     /locus_tag="ACHWN2_002190"
     CDS             complement(8772..9359)
                     /gene="mog"
                     /locus_tag="ACHWN2_002190"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR8931715.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9474..10427)
                     /gene="tal"
                     /locus_tag="ACHWN2_002191"
     CDS             complement(9474..10427)
                     /gene="tal"
                     /locus_tag="ACHWN2_002191"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR8931716.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10706..12136
                     /locus_tag="ACHWN2_002192"
                     /pseudo
     CDS             10706..12136
                     /locus_tag="ACHWN2_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            12206..12982
                     /gene="yaaA"
                     /locus_tag="ACHWN2_002193"
     CDS             12206..12982
                     /gene="yaaA"
                     /locus_tag="ACHWN2_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR8931717.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13135..13431)
                     /locus_tag="ACHWN2_002194"
     CDS             complement(13135..13431)
                     /locus_tag="ACHWN2_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR8931718.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="ACHWN2_002195"
     CDS             complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="ACHWN2_002195"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR8931719.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="ACHWN2_002196"
     CDS             complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="ACHWN2_002196"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR8931720.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="ACHWN2_002197"
     CDS             complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="ACHWN2_002197"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR8931721.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKNISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSLSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18354..18473)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="ACHWN2_002198"
     CDS             complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="ACHWN2_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR8931722.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18688..19374)
                     /locus_tag="ACHWN2_002199"
     CDS             complement(18688..19374)
                     /locus_tag="ACHWN2_002199"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR8931723.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         4721 a         5147 c         4759 g         4913 t
ORIGIN      
        1 attatcagtt tcatcatata agatttgact gcatccaggg cgagcaaggt gaattagttt
       61 ttgaacccag tgacgttgaa tattactttg aaccagtgac gatacaggag tcaggaacca
      121 gcatattaaa aaatgagtta gaaaacagtg aggacggtgc gggtcaaata ggctttcaac
      181 tctccaatga tggtacgaat gaaatccagt atggtaaaag caattactac tcttttcagc
      241 atccccatga aggaagtaat cagattcctc tatttattcg cccgcgtaca tatggaaaca
      301 atgtctcaag cgggcaaata atgagccgag tcaaaatcgt ggtaatgtac aactaatcta
      361 tctcccccac cttaactttg ggggagctgc cgattaacgc accgctggac taaaaagcgc
      421 agaataatcc gtattgcaga ttcgctgtac cgccgggtgc tgaatcatcc gctcagcaaa
      481 aatagcatgg tactcttcca tcacattctc gacgcgacca atttctacga cagttttatc
      541 ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag attgcattgt gcatcgcacc
      601 aaaagctttc atcaaagcgg catcatcaaa ctcgccgagg atttctacgt ttaatccctg
      661 ggagttaaac cagttaagca atttgcgccc taacattgaa cgtcgcccag gaatcaaaag
      721 tcgccgttct tccagacaag ccgggaacgg tttttctggt ggtggatttg tacaccagaa
      781 actcacgcca cattcgccaa ttctcacgga gaacaggcct tcctgctgcg tagagtctat
      841 cggacagtca gaaatgatca tatccagttt atgctgactt aattgctcca gcagcatttc
      901 gtgggtggat tcgaagcagc gaagatgaat gggctcgcct tctaccactg cggcgttaag
      961 tacgctactg accaggcgtt tggaaagtgc atcagccacg ccaacgtcaa acaataaatt
     1021 ggattctttg cgatagttca caatatccag catttcctgg cttaaggtga acattttatc
     1081 ggcatagcga tagaccagtt ctcccagctc gctgggttcg agaccacgtc ccttgcgttt
     1141 aaataatttg ccttgcaggc gctcttccag cgctcgaatc tgtccggtaa tggtttgtgg
     1201 agttaaataa agcgcctccg ctgcgccaac cacggaacct tctttataga catgccagaa
     1261 gtaatacaag tggttgtaat tgatatgaga catgctcatt tctctccctg ataacaatga
     1321 aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc aaactgatgg acgcaaacga
     1381 acgcgtaacc agctgtatcc aattaccgcc gaagagatag aaccgaccag gataccgagt
     1441 ttcgcccagt taatcagttc tggatctacg ctaccaaagg ccaggctggc aataaagata
     1501 gacatagtaa aaccgatacc gcacaggatc cccaccacca taatttgctg ataagtcgtt
     1561 ccctcaggca gatgcgccag tttcaaacgc agcgccaacc agcagaacag actaatcccc
     1621 agcggtttgc caatcagcaa gccagcgatg atccccaatg gcagaatgga ggtcaagcca
     1681 tccagcgtga cgccttgcag tgaaacgcca gcattagcaa atgcaaacag cggcaaaatc
     1741 agatacgcca cccacgggtg caacacatgc tccagtcgct tcgctggaga acgcccatgc
     1801 ttctctttca aaggaataaa gaagccgaca attacccccg ccagagttgc gtgaaccccc
     1861 gatttcaaca ccgcagtcca caacaccacg ccaacaagaa tatagacgcc cgtgcggcgt
     1921 acaccacaca gattcaatac cgcgagtacc gcaattgcta cagccgcgac gccaagggag
     1981 gccatcgata agtcattagt gtagaacaat gcgatgataa tgatggcccc aagatcgtcg
     2041 ataatagcca gagccatcaa aaagatcttc agcactaacg gaacacgact tcccaacagc
     2101 gccagtacac caagtgcaaa agcaatgtca gtagccgccg ggatcgccca cccttcgcgg
     2161 gtaatcggat cggcatagtt aaaagccaga tagagtaatg ccggcacaat catcccacca
     2221 atagcggcga taactggaaa tgcggcctgg cgtaagctgg ctagcgatcc ttgcatcagt
     2281 tcacgtttaa cttccagacc gactaacagg aaaaataccg ccatcagcgc atcatttatc
     2341 cataacagca tgtttttgtt gatttcgagt gaaccaaccc ggagctgaac cggcgtctcc
     2401 agaaagtcgt gataccatcc actggttgcg ccgctgttgg ccataatcat cgccaggata
     2461 gcggcaatga taagaataat gcctcccgag gcatcactgc taaagaatcg atgcagatgt
     2521 ttcacttttt atttctcttt caggtgaata gatcgttttt actattttac cccgcacgaa
     2581 tcatcgttaa aatcagatta tagtgtagga ataattcgtt ttttacgagc cattgaagaa
     2641 tttgcgccat cagcggtgag atgcgttttt gacggctgta agcgctcacg agtgacccta
     2701 agcccagata atcatagctt aaggtgagga ttcctgaggg attaattagg aaaagtaata
     2761 attaatttgt tgtcagtcgg cgatgcttgt taggccaata tttcaaaaat aatccagctt
     2821 gtcgtcaata attgcgatga agagataaat taaagttaaa taattaaatc attaaaaaat
     2881 tacgccacaa aaatgcgagg cgtcttcagg attagaatcg ataaaacaaa atgggttaaa
     2941 gagagcgata taatagcggc gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg
     3001 cagatagaga aaagccccga gtgatatttt accatcaacc cgaggcctcc tatatgctga
     3061 acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc
     3121 ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg
     3181 taacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt
     3241 tcacggctta cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg
     3301 tgctcgcata tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg
     3361 gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga
     3421 gcgcgggctg ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag
     3481 ctgtttctgc ttttcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc
     3541 accctgtgcg ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc
     3601 ggtctgggtt tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat
     3661 ttcgccaccc agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc
     3721 ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg
     3781 tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac
     3841 ccctgccggg attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca
     3901 tttgttgcac ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg
     3961 tacagcgaag aatccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt
     4021 ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt
     4081 cggaatgcgg atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt
     4141 atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc
     4201 gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac caccgccgcc
     4261 catgcctcct tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga
     4321 gtcggtcaga acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc
     4381 accctggtta cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttctgatttc
     4441 acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt
     4501 ttttaaattg cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca
     4561 gtataattac ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt
     4621 cgacaacatc gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct
     4681 gggcatgttg ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca
     4741 ttttcgcttc gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg
     4801 cagactcgat agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct
     4861 tacgggtgct gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact
     4921 tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt
     4981 tcagaccaga agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg
     5041 cggaaacgtg caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc
     5101 cgcgcggtgc cgggttgata ccatctaggt tgaactgacc cagagatttg ttatcagccg
     5161 cacgtttacg ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg
     5221 tagagaacac ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca
     5281 tcacaccgcc catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt
     5341 cttttacgtc accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt
     5401 ccgggttaac gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca
     5461 ttggcatacg agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc
     5521 cagcatcctg cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca
     5581 ggctttccag tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg
     5641 cagtgatgta tggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt
     5701 tttctgccgc ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct
     5761 gatctttctt gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac
     5821 ccaggtgggt atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt
     5881 cgatttcgat aatagaaata tcgaaagtac caccccccag gtcataaacc gcgatagtac
     5941 ggttgccagt gcctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga
     6001 tacgttttac ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat
     6061 cgttaaagta tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt
     6121 cagcggtttt cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct
     6181 ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga
     6241 tggaaacatc acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg
     6301 caaacagggt gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag
     6361 tttcaccatc ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct
     6421 ccagcacgcg aggaatggtg ccatccataa tcgctacaca agagttggta gtacccaggt
     6481 cgataccaat tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt
     6541 gtgagtctgt aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag
     6601 actcactcac tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat
     6661 gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa
     6721 ttgacagcaa ttttattcat ataaagaatg aatcgcagcc aaggcgttca tcaaaaaatt
     6781 gtaataaaaa gaaaagatta cgtgcctgaa tcttctcttt atcagcagta aactagtggg
     6841 tattcatccc cctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt
     6901 ccgccagcct ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac
     6961 tcctcggcgt cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg
     7021 tttgtcgcat tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagtg
     7081 gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag
     7141 aaataaaaga agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac
     7201 gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc
     7261 tgaaaattat cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat
     7321 tacctgaatg attcagatct ggattttctt cagcattgta gtgaggaaca gttggcaaat
     7381 ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc gcctctccag catactgatg
     7441 cgcaacgaac tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag
     7501 ctgattgccg gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga
     7561 cacggtaaat tgtatcgggc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc
     7621 gacaaagaga tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat
     7681 acctggaaga aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg
     7741 gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt
     7801 gtgtcgcatt tgctttccag ccaactgacc cgcattttac gcacccacgc agcaatgagc
     7861 gtacttgggc atggtttgct gcgcggcgcg gggctgggag gccctgtggg cgcagcatta
     7921 aatggggtta aagcggtcag cggcagcacc tatcgcgtga cgattccagc cgtactgcaa
     7981 atcgcctgcc tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa
     8041 attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc
     8101 gccctgaaaa cactacagtt attcagggaa attatttcac cattcattcg atgatgattt
     8161 ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt
     8221 cggcatgacc accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat
     8281 tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga
     8341 gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct
     8401 gacgctggtt gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca
     8461 gttccttggt gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac
     8521 tctgaaaggc gcgcgcgtcc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct
     8581 gctggcgatc ggtaacattg ccggtaatgc cgcgatcatc cactttgccg gctggattgg
     8641 tctgatctgc ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt
     8701 tggtcgcacc gttctgccga ttggtgaatc ccactaattg tcttgagatc cccccgctcg
     8761 gggggatttt tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc
     8821 acttctggtg ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg
     8881 gcaaaaatac catgcaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct
     8941 ttaatagact tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacgccc
     9001 acctgacgcg aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca
     9061 ccaaagccag gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga
     9121 cgcgccgggc cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc
     9181 tcacacaacg tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca
     9241 aacggcgtgg ttagcgccga tgtcagccat tcttccagcg ctggaatgcc tttatcctga
     9301 taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc
     9361 aagatttttc cgtttaaaca atctcgttaa acagaatgat acactgcgaa gggagtgaca
     9421 gacaggcttc agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc
     9481 agatcgccga tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc
     9541 gccagtttat ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg
     9601 atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct
     9661 tcgctctccg ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc acagcctgcc
     9721 agttccagaa tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg
     9781 tgctctttgt agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga
     9841 gcgtactctt tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaacggc
     9901 gagatcagga acacgcccgc ctccgcacaa gcacgagcct gagcgaagga gaacagcagg
     9961 gtcaggttac agttgatacc ttctttttcc agctgttctg cagcacggat accctgccag
    10021 gtagaagcca gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg
    10081 atcaggcgtt ttgctttcgc aattgacgct tcagtgtcat aggaaagacg cgcatcaact
    10141 tcagttgaga tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt
    10201 ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg
    10261 acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa
    10321 gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg
    10381 gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc
    10441 tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt
    10501 cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact
    10561 gcgttgatat cgcccaccat ttttatacaa aacctcatgt atgctacgca gaagttatca
    10621 agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc
    10681 gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct
    10741 ttggggatcg gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac
    10801 cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca
    10861 tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt
    10921 gggtagcggc aacctggccg gcgttgcgct ggcaattacc gccggtggac caggtgccgt
    10981 cttttggatg tgggttgctg cgtttatcgg catggcgacc tcgtttgccg aatgttccct
    11041 tgcacaactt tataaagaac gtgacgtcaa tgggcagttt cgtggcggac cggcatggta
    11101 tatggcgcgc gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat
    11161 cgcctacggc ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt
    11221 ttcttttgat tttcctccgc tggtgacggg cattattctc gctgtctttg ctctgctggc
    11281 aatcactcgt ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc
    11341 gataatctgg gtactgacca gcctggtgat ttgcgtaata aatatcgggc aacttcccca
    11401 cgtcatttgg tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc
    11461 gggatatacc ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga
    11521 ggtgggaatg ggatcgacgc caaacgcggc agcggcagct gcatcctggc ctccgcatcc
    11581 ggcagcacaa gggattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac
    11641 ggcaagcgcc atgctgatat tactggcggg taacggcaca acctacatgc cgctggaagg
    11701 tattcagctt atccagaagg cgatgcgggt gctaatgggt tcctagggtg ctgaatttgt
    11761 taccctcgtg gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga
    11821 aaacaatctc ttctttttac gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg
    11881 caccttcgca acggtcatcg gcggcacctt gctaagcctt ccgctgatgt ggcaactggc
    11941 agatatcata atggcctgca tggctattac caatttgacc gccattttac tgctctcgcc
    12001 tgtggttcat accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt
    12061 gtttgatccg ttgcgttatc cggatatcgg tcgccagctt tctccggacg cgtgggatga
    12121 tgtttcgcag gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg
    12181 cataaatttc ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga
    12241 ttaccaaagc ccgttgacca ccacgcgcta tacgctgccg gagctgttag acaattccca
    12301 gcagttgatc catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgcat
    12361 cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat gactggcagc cagatttcac
    12421 gccggcgaat gcccgccagg cgattctggc gtttaaaggt gatgtctaca ccggcttgca
    12481 ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc
    12541 cggcttgtat ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg
    12601 gatccgtctt gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac
    12661 caacaagctg aacgaggcgc tcgcagcaca aggcgataat gtggtgatta atctggcgtc
    12721 agatgagtat tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccggt
    12781 gttcctcgat gagaagaacg gcaaatttaa gatcatcagc ttctacgcta agaaagcacg
    12841 cggtctgatg agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactgactgg
    12901 ttttaatagc gaaggttact tctttgatga agattcctcc agcaatggcg aactggtgtt
    12961 taaacgctac gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg aacgccttat
    13021 ccggcctaca aaagcatgca gattcaatat attgcagaat taacgtaggc ctgataagac
    13081 gcgccagcgt cgcatcaggc gctgaatgcc ggattgttac ccggcatttg tcatttagcg
    13141 gtgatgtttg cctggaccat gaccgccgtg atgatcatga ggagctttct tatggtggcg
    13201 cggcggtggc ggcggtccgt gtgggtgcca gcgattgcct cgccattcat aatgttgttt
    13261 ccaccagccg tggtcgcgcc agtgacctcc atcccagtaa tagccacgat tatcacgatc
    13321 gcctatctgt aattttactg acgggactaa cgtaatttcc gcagcctgtg ctgccatggg
    13381 agcgaccaga accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc
    13441 cttttatcat tgcccgttat gggtcgatat ccggagatta cgcgatgaga acgacggttg
    13501 ttattctccg caatcctaat ctctaagtta ccgcatttat tgataatttc tcctttttcc
    13561 ctgtcgtttt tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg
    13621 cctgattgag ataatgaata gattttactg atgattcatc atcaatttac gcaacgcaga
    13681 aaaatcggcg ggcagattat gtgaaagcaa gggtaaatca gcacgttctg ccagctcttt
    13741 tggcagatcc aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg
    13801 cgcggtgccg aggaacaagc catattcgcc tggattcaac tggtcacgca gcgcacgata
    13861 cgctacggca gcgtgcggct ccgaggtgta gcccagttct tttaactcac gcattgtctg
    13921 ttgcgtggtt tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg
    13981 gcggaacaac tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga
    14041 taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt
    14101 atcgttcacg ttggtcgcag caataaaacg tttcaccggc agaccgaatg acttcgccag
    14161 cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg
    14221 cgcctcctgc ggcagctgcg caacagcttc aaagtagtag caaatctgcg ccagcaaacg
    14281 gctgatgtta atcgagttag ccgagtttaa ccccagcgcc actttcagtt cttcatcatc
    14341 aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc
    14401 gatattgccg cccaacgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg
    14461 atagaggata accactttca cattcggcaa accgtagaaa gcatgagcca ctgcagctcc
    14521 ggtatcaccg gaggtcgcgg tcagaatggt cactggctta tcatccgcaa tatgggtcag
    14581 catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa
    14641 ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc
    14701 gcgcacgcgc tcttccagga tttcctgtgg gatttcatca ccaataaacg ccgagaggat
    14761 cttcgcactg cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa
    14821 ttccggcagg tcgtgcggaa aaaacagccc ctgatttttg cccaacccct gggttacggc
    14881 ttgcgcaaag ctgacctgct cgttgtgatc tttcagattg tagagtttca tttagttttc
    14941 cagtactcgt gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg
    15001 caggtagttc ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacatag
    15061 agcgaacaag gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac
    15121 cgcctgccgc gcctgccgga agccaggcag taaccgttca cggtagggtt cagcgataac
    15181 atctttcatc agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc
    15241 agccagatgt cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc
    15301 ccgggcttct gccgtcgaga ctttaattcc cggatacgcc agcacccaca gccactcatc
    15361 aaaccctggc acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc
    15421 aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc cttccagctc
    15481 gcccatcaaa gccagcaaac gagtgtcatt aagcggcttg ccgcaatgtt cattcatcgc
    15541 catcagcgcc gcgaccaccg aacaggcgct ggagcctaag cccgaaccga tcggcatatt
    15601 cttttccaga gtcatcgcca ctggaatttg cttgccaagc tcctggcaaa aacgctccca
    15661 gcactgataa acgatatttt cccgtggttc tgacggcagc ttatcggcaa agcgtccgag
    15721 tttgttgaga ctgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc
    15781 atcaacgggt gccaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga
    15841 agccggggca taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca
    15901 gatcggcaaa gacaccggca gcggtaacgt cattgcccgc accatatccg cgcagcacca
    15961 acggcagcgg ctgataatag tggctataaa aggccagggc gttttcgcca tttttcactt
    16021 tgaacagcgg atcattccca tccacttcgg caatcttcac gcggcagaca ccatcttcat
    16081 caatattgcc aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg
    16141 caaagagatc gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt
    16201 taaactctgc gggcagcaca ggttcaattt caatatccgc cagctccagt tcacgtcccg
    16261 tttcacgagc gagaatcaat agtttacgcg ccacatccat accagaaaga tcatctcgcg
    16321 ggtccggttc ggtataaccc atttcccgcg ccagcgtggt cgcctcggag aggctcatgc
    16381 cttcgtctaa cttgccgaag atataagaaa gcgaacctga aagaatgccg gagaacttca
    16441 ccaattcatc acctgcattg agcagatttt gcaggttctc aataaccggt aatccagccc
    16501 caacgttggt gtcatagagg aatttacgcc gcgatttttc cgccgcataa cgcaactgat
    16561 gatagtaatc catcgacgag gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt
    16621 cgcgcaggaa gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga
    16681 ccgggttcag cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct
    16741 ctttggcttg tgcaagttct tcctgccagt tttccagatt aaggccatgc acactagtga
    16801 gcagagcctt cgagttggca acgccgcaga cacgtaagtc gatatgttta ttcttcagcc
    16861 agctttgctg acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca
    16921 caaacacttc gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag
    16981 tggtcgcatc atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa
    17041 tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac
    17101 gcaaggtgcg cataccatca cctaccaccg agataatggc cagccgttcc gtcaccgcca
    17161 gcggctccag taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag
    17221 ctcgcacaca gtcgctttgt ggaacgcaga aactgatact gtattcggaa gatgattgcg
    17281 taatcagcac cacggaaata cgggcgcgtg acattgcagc aaagacgcgc gccgccatgc
    17341 caaccatccc tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg
    17401 aaatgccctt gaccggtaat tcgtcttcat cacggctggc accaatgagc gtacctggtg
    17461 cttgaggatt tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa
    17521 tggtgcgggg gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg
    17581 acatcgactt caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt
    17641 caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg
    17701 agtagtcgga accgttacgt ccaagcacca ccagttcgcc tttttcatta ccggcggtga
    17761 aacctgccat caacaccatg tgatcagccg gaatgcggct tgccgcaata cggcgggtgg
    17821 actcagcaat atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg
    17881 gatcgataac ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgatcg
    17941 acattttctc gccacggcaa atcagcgcag cgttgatgct atccgggcac tgccccaaca
    18001 aactaatgcc atgcaggaca tgttttattt gggcaaattc ctgatcgacg aaagttttca
    18061 attgcgccag cgggaacccc ggctgggcgg cggcgagtcc cgtcaaaagt tcggcaaaaa
    18121 tacgttcggc atcgctgata ttgggtaaag catcctggcc gctaatgttt ttttcaatca
    18181 tcgccaccag gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct
    18241 gcctggcatt gctttccaga atatcggcaa cacgcagaaa acgttctgca tttgccactg
    18301 atgtaccgcc gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa
    18361 aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc
    18421 gttacctgtg gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt
    18481 tgtgtactct gtaattttta tctgtctgtg cgctatgcct atattggtta aagtatttag
    18541 tgacctaagt caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgcta
    18601 tcagacactc tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa
    18661 gctgaaaaat acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag
    18721 caaacggtgc aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca
    18781 gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc
    18841 gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat
    18901 taatgttgct aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata
    18961 atccgccacc atcggcacac cagtaagcac gtcagccaac gctaactctt cgttagtcag
    19021 cccggaatct tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc
    19081 taacagcggc accagttcta ctggcgtggc gtagtaatga tatttcgccc gactacgcgc
    19141 agtggtggcg acagtgaaat cgacatcgtg taacgattca gccagtgtcg ggaaaacttt
    19201 aatattatca ataatatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg
    19261 tgcctgacta tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc
    19321 tgccccaata ttttctgctc tggcgggtgc gaccaggata atcgttatac gcatattgcc
    19381 actcttcttg accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg
    19441 aatttacaaa ttcttaacgt aagccgcaga aaaagccctt tacttagctt aaaaaaggct
    19501 aaactatttt ctgactgcac taacggttta attgttaaaa
//
LOCUS       DAZSNO010000064        19325 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZSNO010000064 DAZSNO010000000
VERSION     DAZSNO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.064"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..122
                     /locus_tag="ACHWN2_002200"
     CDS             <1..122
                     /locus_tag="ACHWN2_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR8931724.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="ACHWN2_002201"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="ACHWN2_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR8931725.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="ACHWN2_002202"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="ACHWN2_002202"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR8931726.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="ACHWN2_002203"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="ACHWN2_002203"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR8931727.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="ACHWN2_002204"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="ACHWN2_002204"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR8931728.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHWN2_002205"
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHWN2_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR8931729.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHWN2_002206"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHWN2_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR8931730.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHWN2_002207"
                     /gene_synonym="manB"
     CDS             6135..7505
                     /gene="cpsG"
                     /locus_tag="ACHWN2_002207"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR8931731.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHWN2_002208"
     CDS             7560..8954
                     /gene="wcaJ"
                     /locus_tag="ACHWN2_002208"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFR8931732.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHWN2_002209"
     CDS             8956..10434
                     /gene="wzxC"
                     /locus_tag="ACHWN2_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFR8931733.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHWN2_002210"
     CDS             10614..11894
                     /gene="wcaK"
                     /locus_tag="ACHWN2_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFR8931734.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHWN2_002211"
     CDS             11891..13111
                     /gene="wcaL"
                     /locus_tag="ACHWN2_002211"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFR8931735.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHWN2_002212"
     CDS             13122..14516
                     /gene="wcaM"
                     /locus_tag="ACHWN2_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFR8931736.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14691..15584
                     /gene="galF"
                     /locus_tag="ACHWN2_002213"
     CDS             14691..15584
                     /gene="galF"
                     /locus_tag="ACHWN2_002213"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFR8931737.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHWN2_002214"
     CDS             15957..17042
                     /gene="rfbB"
                     /locus_tag="ACHWN2_002214"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR8931738.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHWN2_002215"
     CDS             17042..17941
                     /gene="rfbD"
                     /locus_tag="ACHWN2_002215"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFR8931739.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHWN2_002216"
     CDS             18000..18878
                     /gene="rfbA"
                     /locus_tag="ACHWN2_002216"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR8931740.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            18883..>19325
                     /gene="rfbC"
                     /locus_tag="ACHWN2_002217"
     CDS             18883..>19325
                     /gene="rfbC"
                     /locus_tag="ACHWN2_002217"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFR8931741.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGIL"
BASE COUNT         4803 a         4681 c         5270 g         4571 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctag gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gcactgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat acccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgagc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgatttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacctgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgtgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacctacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tggtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt
     6001 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
     6061 cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt cagggccaac tattgcctga
     6121 aaaagggtaa cgacatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
     6181 taggcgaaga actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc
     6241 tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct caccagcgaa accttaaaac
     6301 tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt gctggatatc ggtatgtccg
     6361 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
     6421 ctgccagcca taatccgatg gattacaacg gcatgaagct ggtgcgcgag ggggctcgca
     6481 cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct ggctgaagcc aacgactttc
     6541 ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat caacctgcgt gacgcttacg
     6601 ttgatcacct gttcggttat atcaatgtca aaaatctcac gccgctcaag ctggtgatca
     6661 actccgggaa tggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagccc
     6721 tcggcgcgcc cgtggaatta atcaaagtac acaacacacc ggacggcaat ttccccaacg
     6781 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac
     6841 acggcgcgga tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg
     6901 aaaaagggca gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg
     6961 aaaaaaatcc cggcgcgaag atcatccatg atccgcgtct ctcttggaac actattgatg
     7021 tggtgactgc tgcaggcggc acgccggtaa tgtcgaaaac cggacacgcc tttattaaag
     7081 aacggatgcg caaggaagac gccatctacg gtggcgaaat gagcgcccac cattacttcc
     7141 gtgatttcgc ttactgcgac agcggcatga tcccgtggct gctggtcgcc gaactggtgt
     7201 gcctgaaagg aaaaacgctg ggcgaactgg tgcgcgaccg gatggcggcg tttccggcaa
     7261 gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc gattaaccgc gtggaacagc
     7321 attttagccg tgaggcgctg gcggtggatc gcagcgatgg catcagcatg acctttgccg
     7381 actggcgctt taacctgcgc acctccaata ccgaaccggt ggtgcgcctg aatgtggaat
     7441 cgcgcggtga tgtgccgctg atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg
     7501 agtaatgtcg gatcttccct taccccactg cgggtaaggg gctaataaca ggaacaacga
     7561 tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc atcgttaatc tctatggtgc
     7621 aacgcttttc agatatcacc atcatgtttg ccgggctatg gctggtttgc gaagtcagcg
     7681 gactgtcatt cctctacatg cacctgttgg tggcgctgat tacgctggtg gtgttccaga
     7741 tgctgggcgg catcaccgat ttttatcgct catggcgcgg tgttcgggca gcgacagaat
     7801 ttgccctgtt gctacaaaac tggaccttaa gcgtgatttt cagcgccgga ctggtggcgt
     7861 tcaacaatga tttcgacacg caactgaaaa tctggctggc gtggtatggg ctgaccagca
     7921 tcggactggt ggtttgccgt tcatgtattc gcattggggc gggctggctg cgtaatcatg
     7981 gctacaacaa gcggatggtc gccgtagcgg gggatttagc cgccggacaa atgctgatgg
     8041 agagtttccg taatcagccg tggttagggt ttgaagtggt gggcgtttac cacgacccga
     8101 aaccgggcgg cgtttctaac gactgggcgg gtaacctgca acagctggtc gaggatgcaa
     8161 aagcgggcaa gattcataac gtctatatcg cgatgcaaat gtgcgacggc gcacgagtga
     8221 aaaaactggt ccatcaactg gcggatacca cctgttcggt gctgctgatc cccgacgtct
     8281 ttaccttcaa cattctccat tcacgtctcg aagagatgaa cggcgttccg gtggtgccgc
     8341 tgtatgacac gccgctttcg ggggttaacc gcctgctaaa acgtgcggaa gacattgtgc
     8401 tggcgacgct tattctgctg ctgatctccc cggtgctgtg ctgtattgcg ctggcggtga
     8461 aactcagttc accagggccg gttattttcc gccagactcg ctatggtatg gatggcaagc
     8521 cgatcaaagt gtggaagttc cgttccatga aagtgatgga gaacgacaaa gtggtgaccc
     8581 aggcgacgca gaacgatccg cgcgtcacta aagtggggaa ctttctgcgc cgcacctcgc
     8641 tggacgaatt gccgcagttt atcaatgtgc tgaccggggg gatgtcgatt gtcggtccac
     8701 gtccgcacgc agtagcgcat aacgaacagt atcgccagct cattgaaggc tacatgctgc
     8761 gccataaagt gaaaccgggc attaccggct gggcgcagat taatggctgg cgcggcgaaa
     8821 ccgacacgct ggagaaaatg gaaaaacgcg tcgagttcga ccttgagtac atccgcgaat
     8881 ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt attcaagggc ttcgttaaca
     8941 aagcggcata ttgatatgag cttacgtgaa aaaaccatca gcggcgcgaa gtggtcggct
     9001 attgccacgg tgatcatcat cggcctcggg ctggtgcaga tgaccgtgct ggcgcggatt
     9061 atcgacaacc accagttcgg cctgctgact gtgtcgctgg tgattatcgc gctggcagat
     9121 acgctttctg acttcggtat cgctaactcg attattcagc gaaaagaaat cagtcacctt
     9181 gaactcacca cgctgtactg gctgaacgtc gggctggggc ttgtggtatg tgtggcggtg
     9241 tttttgttga gtgatgtcat cggcgacgtg ctgaataacc cggacctggc accgttgatt
     9301 aaaacattgt cgctggcgtt tgtggtgatc ccccacgggc aacagttccg cgcgttgatg
     9361 cagaaggagc tggagttcaa caaaatcggc atgatcgaaa ccagcgcggt gctggcgggc
     9421 ttcactttta cggtagttag cgcccatttc tggccgctgg cgatgaccgc gatcctcggt
     9481 tatctggtca atagtgcggt gagaacactg ctgtttggct actttggacg caaaatttat
     9541 tgccccggtc tgcacttctc gctggcgtct gtggcaccga acttacgttt tggtgcctgg
     9601 ctgacggcgg acagcatcat caactatctc aacaccaacc tttcaacgct ggtgctggcg
     9661 cgtattcttg gcgcgggcgt ggcaggggga tacaacctgg cgtacaacgt ggccgtcgta
     9721 ccgccgatga agctgaaccc aatcatcacc cgcgtgttgt ttccggcgtt tgccaaaatt
     9781 caggacgaca ccgaaaagct gcgtgttaac ttctacaagc tgctgtcgat agtggggatt
     9841 atcaactttc cggcgctgct ggggctgatg gtggtgtcga ataacttcgt accgctggta
     9901 ttcggcgaaa agtggaacag cattattccg gtgctgcaat tgctgtgtat tgtgggtctg
     9961 ctgcgctccg tgggtaaccc gattggttcg ctgctgatgg cgaaagcgcg ggtcgatatc
    10021 agctttaaat tcaacgtatt caaaacattt ctgtttattc cggcgattgt tattggtggg
    10081 cagatggcgg gcgcaatcgg cgtcacgctc ggtttcctgc tggtgcagat tatcaacacc
    10141 attctgagtt acttcgtgat gattaaaccg gttcttggtt ccagttatcg ccagtacatc
    10201 ctgagtttgt ggctgccgtt ttatctctcg ttgccgacgc tggtggtcag ttatgcgctg
    10261 ggcattgtgc tgaaagggca actggcgctg gggatgctgc tggcggtgca aatagccgcg
    10321 ggcgtgctgg cgtttgtggt gatgattgtg ctgtcacgcc atccgctggt ggtggaagtg
    10381 aagcgtcagt tttgtcgcag cgaaaaaatg aaaatgcttt tacgggcggg gtgaattgct
    10441 gttttcggtt ccgacaacgg ttgccggatg cggcgtaaaa cgccttatcc gtcctacggt
    10501 tctgtgctcg ggacctgtag gcctgataag acgcgccagc gtcgcatcag gcaaaaaacg
    10561 gcaacgaaca caacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    10621 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    10681 tacttgatgc catcaacatc ctcaatccac ataccgaagt ggacgtgatg agccgctatc
    10741 cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg ttcctgcaga
    10801 tgacacaaca caatagcgcg gcgggcgttg tcgggcgcat taaaaaagtc ctccgtcgcc
    10861 gctaccagca tcaggtattg ctctcacgcg tcaccgacac tggcaagctg cgcaatatcg
    10921 ccatcgccca gggattcacc gacttcgtgc gcctgctgtc aggttacgac gccattattc
    10981 aggtcggcgg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcgcttt
    11041 gcacatttat ggcgaaaaag ccgctgttta tgattggtca cagcgtcggc cccttccagg
    11101 atgagcaatt taaccaactg gcgaactatg tctttggtca ctgcgacgcg ctgatcctgc
    11161 gcgaatcggt cagccttgat ttgatgaaac gcagaaatat caccaccgca aaagtggaac
    11221 atggcgtcga taccgcgtgg ctggtcgatc accacaccga agacttcacc gccagctatg
    11281 ccgttcaaca ctggctggac gttgccgcac aacagaaaac agtggcaatt accctgcgcg
    11341 aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa aaagcctttg
    11401 ccggggtggt caatcgcatt ctcgatgaag gctatcaggt gattgcgctc tccacctgta
    11461 cgggtatcga cagctataac aaagacgacc gcatggtggc gctcaatctg cgccagcaca
    11521 tcagcgatcc tgcccgttac cacgtagtga tggatgaact caacgatctg gaaatgggca
    11581 aaattctcgg agcctgtgaa ctcaccgtcg gtacgcgcct gcactccgcc attatctcaa
    11641 tgaactttgc tactccggcg attgccatca actatgaaca taaatccgcc gggattatgc
    11701 agcagctggg actgccagag atggcgattg atattcgtca tttactggac ggcagcctgc
    11761 aagcgatggt tgcggatacc ttaggccagc ttccggtgct gaacgctcga tttaacgaag
    11821 ccgtcagtcg cgaacgtcag acgggaatgc agatggtgca gtctgtgctt gagcgcatcg
    11881 gggaggtgaa atgaaggtcg gcttcttctt attgaaattc ccgctgtcgt cggaaacctt
    11941 cgtcctcaat caaattaccg cgtttattga tatgggcttt gaagtggaga ttgtcgcttt
    12001 gcaaaaaggc gatacagaga acacccacgc ggcatggacg aaatacaacc ttgtcgccag
    12061 aacccgctgg ttacaggacg aacctgcggg caaagtagcg aaactgcgcc accgcgccag
    12121 ccagacctta cgtggcattc atcgtaaaaa tacctggcat gcgctcaacc tcaaacgcta
    12181 tggtgccgag tcgcggaacc tgattttgtc tgccatttgt ggtcaggtcg caacaccgtt
    12241 tcgcgccgat gtgttcatcg ctcattttgg cccggcgggg gtaaccgcag caaaactacg
    12301 cgaactgggt atcattcgcg gcaaaattgc caccatcttc cacggtattg atatctccag
    12361 tcgggaagtg ctcaaccact acactcccga atatcaacaa ctgtttcgcc gtggcgacct
    12421 gatgttaccg ataagcgatc tgtgggccgg aaggctgcaa aaaatgggct gccccagaga
    12481 aaaaatcgcc gtatcgcgta tgggcgtgga catgacgcgc tttagcccgc gtccggtgaa
    12541 agcgcccgca acgccgctgg aaatcatatc tgtcgcacgc ttaaccgaga aaaaaggcct
    12601 gcatgtggcg attgaagcct gccgtcagtt gaaagagcag ggcgtggcat ttcgctatcg
    12661 catcctcggc attggcctgt gggaacgacg cctgcgcacc atcatcgaac aatatcaact
    12721 ggaagatgtg gtagagatgc cgggctttaa accgagccat gaagtgaagg agatgctcga
    12781 cgacgcggat gtcttcctgt tgccatcggt aacgggcgcg gatggcgata tggaaggcat
    12841 tccggtggcg ctaatggaag cgatggcggt cggtattccg gtggtttcta ctctgcatag
    12901 cggaattcct gaactggtgg aggccgataa atccggctgg ctgctgcctg agaacgatgc
    12961 tcgcgcactg gcacaacgtc tggcggcgtt tagccaactg gataccgacg aactggctcc
    13021 ggtcgtcaaa cgtgcgtgcg aaaaagtcga acacgatttt aaccagcagg tgattaatcg
    13081 agaactcgcc agcttgctgc aggctttata gaggagaacg tatgccattt aaaacactct
    13141 cccgacgcac cttcctgacg gcaagctcgg cgcttgcctt cctccatacc cctttcgctc
    13201 gcgcacttcc cgcccgacaa agcgttaaca ttaacgacta caacccacat gactggatcg
    13261 cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc ggattcgttt
    13321 gtgacaatat caacaccggc atcttcatcc ctcccggcaa aacgttacac atccttggaa
    13381 gcctgcgcgg caacggcaga gggcgatttg tcttacagga cggcagccag gtgacagggg
    13441 gagagggcgg cggtatgcat aacattaccc tggatgtgcg tggttctgac tgcaccatca
    13501 aagggctggc gatgagcggc tttggcccgg taatgcagat ttatattggc ggcaaaaaca
    13561 aacgggtcat gcgcaacctg accatcgata acctcactgt cagccacgct aattacgcca
    13621 tcttacgcca gggatttcat aaccagatta tcggtgccaa cattaccaac tgtaagttca
    13681 gcgacttaca gggcgacgcc attgaatgga acgtggcaat taacgacagt gatattttga
    13741 tatctgacca tgtcatcgag cgtatcaact gtaccaacgg caaaatcaac tggggcatcg
    13801 gcataggcct tgcgggaagc acttatgata acaactaccc ggaagaccag gcagtgaaaa
    13861 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat gttgaaaatg
    13921 gtaaacattt tgttattcgt aatatcaatg cccgcaatat cacgccggat ttcagtaaga
    13981 aagcgggcat tgataacgcg acagtcgcta tttacggttg tgacaatttc gtgattgata
    14041 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaaat
    14101 atctctcgat accgcaaaat ttccgagtga ataatattca actggataat acccatcttg
    14161 cttataaatt gcgcggcatc caaatctccg ccgggaatgc cgtctccttt gtgtcgctaa
    14221 ctaacattga gatgaagcgt gcctcgctgg agttacacaa caagccgcaa catcttttta
    14281 tgcgaaatat caatgtgatg caggaatcct cagttggacc agcattgagc atgaacttcg
    14341 acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg ctgtctcttg
    14401 caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac aggattaatc
    14461 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg gagtagattt
    14521 gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca atccacaacg
    14581 aagcggcgta acatcacaag taattcagta atcaattcag ggtaattgat gctggcgaaa
    14641 aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata atgacgaatt
    14701 taaaagcagt tattcctgta gcgggtctcg ggatgcatat gttgcctgcc actaaggcga
    14761 tacccaaaga gatgctacca atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    14821 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
    14881 aaaaccactt cgacacctct tatgagttag aatcactcct tgagcagcgc gtgaagcgtc
    14941 aactgctggc ggaagtacag tccatctgtc cgccgggcgt gatcattatg aacgtgcgtc
    15001 agggcgaacc tttaggttta ggccactcca ttttgtgtgc gcgacctgcc attggtgaca
    15061 acccatttgt cgtggtactg ccagacgttg tgatcgacga tgccagcgcc gacccgctac
    15121 gttacaacct tgctgccatg attgcacgtt tcaacgaaac gggccgcagc caggtgctgg
    15181 caaaacgtat gccgggtgac ctctctgaat actccgtcat ccagactaaa gagccgctgg
    15241 accgtgaggg taaagtcagc cgcattgttg aatttatcga aaaaccggat cagccgcaga
    15301 cgctggactc agacatcatg gccgtaggtc gctatgtgct ttctgccgat atttggccgg
    15361 aactggaacg tactcagcct ggtgcatggg gacgtattca gctgactgat gctattgccg
    15421 agctggcgaa aaaacaatcc gttgatgcaa tgctgatgac cggcgacagt tacgactgcg
    15481 gcaaaaaaat gggctatatg caggcgtttg tgaagtatgg cctacgcaac ctgaaagaag
    15541 gggcgaagtt ccgtaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    15601 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcataa gttttgctca
    15661 ttgaattttc ctgccgttgc cgtttttata aagcctataa aaacaagaag ttacatagct
    15721 catattattc aaatttttcc aggattttcc ttgtttctac ggctgtttgg taagacaatt
    15781 aacgtttgaa tttttcggat ttggcgcgag tggctaacgc tcgccacatc gttgagatgc
    15841 atgcagtgca ctggtagctg ttaagccagg ggcggtagcg tgtattaata cctctattaa
    15901 tcaaatctgg agcagtctat ttcacagcat gctctatagc tatatggaat aaaaaagtga
    15961 agatacttgt tactggtggc gcagggttta ttggttctgc tgttgttcgt cacataataa
    16021 ataatacgca agatagtgtt gttaatgtcg ataaattaac atacgccggg aacctggaat
    16081 cactcgctga tgtttctgat tctgaacgtt atgcttttga gcatgcagat atctgcgatg
    16141 ctgtagccat gtcgcgtatt ttcgcacagc accagccaga cgcggtgatg cacctggcgg
    16201 cagagagcca cgttgaccgt tcaataacgg gccctgcggc atttattgaa accaatattg
    16261 tggggactta tgttctttta gaagcggcgc gcaattactg gtctgctctg aatgatgaaa
    16321 agaaaaaaag tttccgcttt catcatattt ctactgatga agtttatggt gacttacccc
    16381 atccggatga agcaaataat aacgaagcgt taccgctatt tacggaaacg acagcttacg
    16441 cgccaagtag cccgtattct gcgtctaaag cttccagcga tcatttggta cgtgcctgga
    16501 agcggactta tggtttacca accattgtga ctaattgctc gaacaattat ggtccttatc
    16561 acttcccgga aaaacttatt ccattggtta ttctgaatgc tctggaaggt aaggcattac
    16621 ctatttatgg taaaggggat caaattcgcg actggttgta tgtagaggat catgctcgtg
    16681 cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacata
    16741 acgaaaagaa aaacatcgac gtagtgctca ctatttgtga tttgttggat gagattgtac
    16801 cgaaagagaa atcttatcgt gagcaaatta cttatgttgc cgatcgcccg gggcatgacc
    16861 gccgttacgc cattgatgcc gataaaatta gccgcgaatt gggctggaaa ccgcaagaaa
    16921 cgtttgagag cgggattcgt aaaacggtgg aatggtatct ggctaataca aattgggttg
    16981 agaatgtgaa aagcggtact tatcagtcgt ggattgaaca aaactatgag ggccgtcagt
    17041 aatgaatatc ctgcttttcg gcaaaacagg gcaggtgggt tgggaactgc agcgtgctct
    17101 ggctcctctg ggtaacttga ttgcgctcga tgttcactcc actgattatt gtggagattt
    17161 cagcaacccc gaaggtgtgg ctgaaaccgt caaaaaaatt cgccccgatg ttattgttaa
    17221 tgctgctgct cacactgcag tagacaaagc ggaatcagaa ccgaatttcg cgcaattact
    17281 taacgcgaca tgcgtcgaag cgattgcaaa agcagctaat gaagtcggag cctgggttat
    17341 acactactcc actgattatg ttttcccagg caacggtgac acgccatggc tggaaacgga
    17401 tgcaacagca ccgctaaatg tctacggtga aaccaagcta gctggggaaa aagcgttaca
    17461 agaacattgc gcaaagcatc ttattttccg taccagctgg gtatacgctg gtaaaggaaa
    17521 taactttgcc aaaacgatgt tgcgcctggc aaaagaacgc gaagagttgg ctgtaataaa
    17581 cgatcaattc ggcgcaccaa cgggggctga attactggct gattgcaccg ctcatgccat
    17641 tcgcgtggcc gcaaataaac cagaagttgc tggtttgtac catctggttg ctggtggtac
    17701 aacaacctgg cacgattatg ccgctctggt atttgaagaa gcgcgcagag cggggatcaa
    17761 ccttgctctt aacaaactta atgccgtgcc gacaacggcc tatcccacac cagcacgtcg
    17821 tccccataat tctcgcctca ataccgaaaa atttcagcag aactttgcgc ttgtcttgcc
    17881 tgactggcag gtgggcgtga aacgtatgct caacgaatta tttacgacca cggcaattta
    17941 acaaattatt gcatctcgct catgatgcta gagcgagatg aattaaaagg aatggtgaaa
    18001 tgaaaacgcg taaaggtatt attctggctg gtggttccgg cactcgtctt tatcctgtga
    18061 caatggctgt gagcaaacaa ctgctaccga tttatgataa accgatgatt tattatccgc
    18121 tttcaacgct tatgttggcg ggtattcgag atattctgat tatcagtacg ccgcaggata
    18181 caccacgttt tcaacaattg cttggtgatg gaagccagtg ggggcttaat ctgcaatata
    18241 aagtacaacc gagtccggat ggtctggcgc aagcatttat cattggtgaa gaatttatag
    18301 gtggtgatga ttgtgcgctg gtacttggtg ataatatctt ctatggtcac gacctgccga
    18361 aattaatgga taccgctgtt aacagagaga gtggcgcaac ggtttttgcc tatcacgtaa
    18421 atgatcctga gcgctacggt gtggtggaat ttgatgataa cggtacggca attagtctgg
    18481 aagaaaaacc gcaggaacca aaaagtaact atgcggttac tgggctttat ttctatgaca
    18541 atgacgttgt agaaatggcg aaaaacctta aaccatctgc acgtggcgaa ctggaaatta
    18601 ccgatattaa ccgtatttat atggaccaag gacgtttgtc tgtcgctatg atggggcgtg
    18661 gttatgcatg gctggacacg gggacacatc aaagtcttat tgaagcaagc aacttcattg
    18721 caacaattga agagcgtcag gggctgaaag tttcctgccc ggaagaaatt gctcaccgta
    18781 aaggctttat tgatgctgag caagtgaagg tattagccga gccgctgaag aaaaatgctt
    18841 atggtcagta tctgctgaaa atgattaaag gttattaata aaatgaacgt aattaaaact
    18901 gaaattcctg atgtgctaat ttttgaacca aaagtatttg gtgatgagcg gggctttttt
    18961 atggaaagct tcaatcagaa agttttcgaa gaggctgtag gacgaaaggt tgagtttgtt
    19021 caggataacc attctaaatc aactaagggt gtgttacgcg gactgcacta tcagctggaa
    19081 ccttatgctc aaggaaaact ggtacgctgt gttgtcggtg aagtttttga cgtagctgtt
    19141 gatattcgga aatcatcacc aacatttggc aagtgggttg gtgtaaattt gtcggcggag
    19201 aataagcgtc aattatggat accggaagga ttcgctcatg ggttttgcgt tcttagtgat
    19261 gaagcagaat ttgtctacaa aacaaataat ttctattcaa aaatgcaaga gcgtgggatt
    19321 ttatg
//
LOCUS       DAZSNO010000065        19061 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZSNO010000065 DAZSNO010000000
VERSION     DAZSNO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19061
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.065"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1025)
                     /locus_tag="ACHWN2_002218"
     CDS             complement(<1..1025)
                     /locus_tag="ACHWN2_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8931742.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="ACHWN2_002219"
     CDS             complement(1127..2107)
                     /locus_tag="ACHWN2_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR8931743.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="ACHWN2_002220"
     CDS             complement(2115..2825)
                     /locus_tag="ACHWN2_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR8931744.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHWN2_002221"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHWN2_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR8931745.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="ACHWN2_002222"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="ACHWN2_002222"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR8931746.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="ACHWN2_002223"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="ACHWN2_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR8931747.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="ACHWN2_002224"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="ACHWN2_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR8931748.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="ACHWN2_002225"
     CDS             9402..9512
                     /locus_tag="ACHWN2_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR8931749.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHWN2_002226"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHWN2_002226"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR8931750.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHWN2_002227"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHWN2_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR8931751.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11931..12097)
                     /locus_tag="ACHWN2_002228"
                     /pseudo
     CDS             complement(11931..12097)
                     /locus_tag="ACHWN2_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12394..13026
                     /gene="yihA"
                     /locus_tag="ACHWN2_002229"
     CDS             12394..13026
                     /gene="yihA"
                     /locus_tag="ACHWN2_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR8931752.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHWN2_002230"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHWN2_002230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR8931753.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="ACHWN2_002231"
     CDS             16558..17490
                     /locus_tag="ACHWN2_002231"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR8931754.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..18961)
                     /locus_tag="ACHWN2_002232"
     CDS             complement(17531..18961)
                     /locus_tag="ACHWN2_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR8931755.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         4661 a         4807 c         4564 g         5029 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcacttgcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacgctcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg cggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt
    18181 tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt
    18241 ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt
    18301 tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    18361 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    18421 aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg
    18481 caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    18541 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    18601 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    18661 gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga
    18721 gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt
    18781 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat
    18841 cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg
    18901 tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca
    18961 tataaatatt ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt
    19021 ccagcggcag gatgcattat cagaatgtat ttatatttat t
//
LOCUS       DAZSNO010000066        18804 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZSNO010000066 DAZSNO010000000
VERSION     DAZSNO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18804
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.066"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(139..3654)
                     /locus_tag="ACHWN2_002233"
     CDS             complement(139..3654)
                     /locus_tag="ACHWN2_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR8931756.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="ACHWN2_002234"
     CDS             complement(4107..5369)
                     /locus_tag="ACHWN2_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8931757.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="ACHWN2_002235"
     tRNA            complement(5567..5642)
                     /locus_tag="ACHWN2_002235"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="ACHWN2_002236"
     CDS             complement(5748..6455)
                     /locus_tag="ACHWN2_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR8931758.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6608..6691
                     /gene="yqgH"
                     /locus_tag="ACHWN2_002237"
     CDS             6608..6691
                     /gene="yqgH"
                     /locus_tag="ACHWN2_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR8931759.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="ACHWN2_002238"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="ACHWN2_002238"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR8931760.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="ACHWN2_002239"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="ACHWN2_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR8931761.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="ACHWN2_002240"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="ACHWN2_002240"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR8931762.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="ACHWN2_002241"
     CDS             complement(11639..11914)
                     /locus_tag="ACHWN2_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR8931763.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="ACHWN2_002242"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="ACHWN2_002242"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR8931764.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="ACHWN2_002243"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="ACHWN2_002243"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR8931765.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="ACHWN2_002244"
     CDS             13874..14200
                     /locus_tag="ACHWN2_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR8931766.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="ACHWN2_002245"
     CDS             14384..15103
                     /locus_tag="ACHWN2_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR8931767.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="ACHWN2_002246"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="ACHWN2_002246"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR8931768.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="ACHWN2_002247"
     CDS             16442..17404
                     /locus_tag="ACHWN2_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR8931769.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18804)
                     /locus_tag="ACHWN2_002248"
     CDS             complement(17505..>18804)
                     /locus_tag="ACHWN2_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFR8931770.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4899 a         4585 c         4644 g         4676 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cgatataact atcc
//
LOCUS       DAZSNO010000067        18721 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZSNO010000067 DAZSNO010000000
VERSION     DAZSNO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18721
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.067"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            372..575
                     /locus_tag="ACHWN2_002249"
                     /pseudo
     CDS             372..575
                     /locus_tag="ACHWN2_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            594..3980
                     /locus_tag="ACHWN2_002250"
     CDS             594..3980
                     /locus_tag="ACHWN2_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR8931771.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            3983..4363
                     /locus_tag="ACHWN2_002251"
     CDS             3983..4363
                     /locus_tag="ACHWN2_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931772.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(4418..4705)
                     /locus_tag="ACHWN2_002252"
     CDS             complement(4418..4705)
                     /locus_tag="ACHWN2_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFR8931773.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            4834..5343
                     /locus_tag="ACHWN2_002253"
     CDS             4834..5343
                     /locus_tag="ACHWN2_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR8931774.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            5340..6758
                     /gene="phrB"
                     /locus_tag="ACHWN2_002254"
     CDS             5340..6758
                     /gene="phrB"
                     /locus_tag="ACHWN2_002254"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR8931775.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(6908..8389)
                     /gene="dtpD"
                     /locus_tag="ACHWN2_002255"
     CDS             complement(6908..8389)
                     /gene="dtpD"
                     /locus_tag="ACHWN2_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR8931776.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            8660..9403
                     /gene="ybgI"
                     /locus_tag="ACHWN2_002256"
     CDS             8660..9403
                     /gene="ybgI"
                     /locus_tag="ACHWN2_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR8931777.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            9426..10082
                     /gene="pxpB"
                     /locus_tag="ACHWN2_002257"
     CDS             9426..10082
                     /gene="pxpB"
                     /locus_tag="ACHWN2_002257"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR8931778.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            10076..11008
                     /gene="pxpC"
                     /locus_tag="ACHWN2_002258"
                     /pseudo
     CDS             10076..11008
                     /gene="pxpC"
                     /locus_tag="ACHWN2_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            10998..11732
                     /gene="pxpA"
                     /locus_tag="ACHWN2_002259"
     CDS             10998..11732
                     /gene="pxpA"
                     /locus_tag="ACHWN2_002259"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR8931779.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            11768..12559
                     /gene="nei"
                     /locus_tag="ACHWN2_002260"
     CDS             11768..12559
                     /gene="nei"
                     /locus_tag="ACHWN2_002260"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR8931780.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(12556..13602)
                     /locus_tag="ACHWN2_002261"
     CDS             complement(12556..13602)
                     /locus_tag="ACHWN2_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR8931781.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(13754..14830)
                     /locus_tag="ACHWN2_002262"
                     /pseudo
     CDS             complement(13754..14830)
                     /locus_tag="ACHWN2_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(14827..15558)
                     /locus_tag="ACHWN2_002263"
     CDS             complement(14827..15558)
                     /locus_tag="ACHWN2_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR8931782.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            complement(15573..18032)
                     /locus_tag="ACHWN2_002264"
     CDS             complement(15573..18032)
                     /locus_tag="ACHWN2_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8931783.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            complement(18083..18649)
                     /locus_tag="ACHWN2_002265"
     CDS             complement(18083..18649)
                     /locus_tag="ACHWN2_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8931784.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
BASE COUNT         4635 a         4477 c         5162 g         4447 t
ORIGIN      
        1 gttaataaaa agcttataga tttatagttg ataattaaaa cgcatccgat atttcagctt
       61 tttctatgca agatacaatt ttataaggtg tccattttta tatggaggcg gtagcgtgtc
      121 tgttactagt ggttttattt gcatagtgaa tatattggga ttgttaaata cataactatg
      181 tatccgctct ggtcgttgta aatattgcta attgcttaat attattagtg attatgactg
      241 gaaagataaa gtgtgctgta aagcggtgat gggtattaca ttcatgatta aaaaaacaga
      301 acctgtcaat aaataattcc tttattaaaa cggactattt acattatgac ggaatattat
      361 ctcaacgagg cggtttggaa atacagagcc agtttctccg gggtaacgag caggtgtacc
      421 agtgtcaggt ggcatttgta ttgccgggtg agcgggtcat gatggccttc acctacgcca
      481 gaaccacacc gctgaccccg gcagatatga cgcgctgggc tgaaattaaa aagaatctgc
      541 gcttcaggat gcggcaggaa gtcaggacga attaatcata cagggaacgg gacatggaag
      601 aggcattctg ggcggcaaga cagggtgatg ccctgctgca tacgtcgttt atggcagatg
      661 tgctgggggc ggtgctggag gtggccgcaa atgtcgttat tgatgccctc attgtcggca
      721 caacctcgtt actggcgacg tcagtgggga tcacgatggg gtgcacggcc gtcgtgctga
      781 ccggttttgt ggcgggtgtg gcgatggtgt acaccggcgt gacagaaaag gtcagcgaag
      841 cctgttcagc cctcgcaaat atgcttttcc cgcctcagat tgaaggttac atcctcaccg
      901 gctctggtga cacatggatc aacagcaaac ctgccgcgcg ggcagcggcg actgcggcat
      961 cgcggcagga catagaggcg caggaagcgc aggcgaaggc agaacaggaa gaagcgcagc
     1021 gtcaggagga tgccagaacg ttccgggatg tggccgggga atatctggaa atggcggggg
     1081 ccatagccct ggcggttaac ccggtgacag ggcctgtgct gatgtcccgg gctctgcacg
     1141 accagctcag tacggaagaa gggcgttctg aaatggtgga tggcgtgaca cattttgtgt
     1201 cggaactatg gcagcccact gtggccagtg cggccccggg atccaccccg acccctgacg
     1261 ataaaatcga ctgccataaa catccttcct cactgacgca gtttctcgca caaaaaaaag
     1321 ccgcctttct ggatgacccc ctgggaacgg cccttaacct gctgaatccg ggcggtatgc
     1381 tggaaaccgc ctttcaggga gtgaatgcgg ttattggcag catcagtaat ctgttcaaag
     1441 gcgatgacga gccgcccgca gcggaatata tcgcggaagg cgcgcgggat gtgcgtatca
     1501 acagccagcc ggcgacacgc agcggggcac gctgtacctg cgaggccagg gtggtgaatg
     1561 agccggggaa cggggcgttt gtgtcaccgg atgtgcgcat tggcggtccg ttactggtgg
     1621 tcagggatat cagaagcggc aggtcgcaga taaccctggt ggcaaccgtg gcgctgatgt
     1681 ttcttcgccc gggcaaaatg ctttctaaaa tcgcctgttt tgctgccagt gtcgggatga
     1741 gcatgctgac gcagaaggcc accagtgccc ttccgcatcc ggtcaatgcc gccaccgggg
     1801 ccaaatacct ggccgatgac gatgacttcg atttcagtct gccgggccac tttccgctgg
     1861 actggcagcg cgtgtacagc agtcgtgacg aacgtacgga aggaatgttt ggtcagggct
     1921 ggagtgtaat gtatgaagtc agcctcgtgt gcacaccggg tagcgcagac gaaaactgca
     1981 tgacctttgt gtcgggcatg gggcgacgct tggatatgga agccgtactg ccggggggcg
     2041 ggttttacag ccccggtgaa gggctggcgg taaggcgtgg tgagcagggg cactggctta
     2101 tcagcagcga tgacgggcag tgttttctgt ttgaggctga cccgcaccac ccgcagcgcc
     2161 agcggctgaa aatgctgggc gaccgtaaca gcaactgtct gaatctgtac tacgacgacc
     2221 ggggccgcat tgtggaaatc agcggcgaac agcagcgtcc gtgcatccgt ctgtattacg
     2281 aactggcggc gcatccccgg cgggtgacac agatttatca gcatttccct gagactgcac
     2341 ctttactgct gcgtcgttac agctatgacg aggccggcca cctgaacggc gtgtacgaca
     2401 gcacggggca cctgctgcgt gagtttgctt acgacgaaaa ccactgcatg acgctgcacc
     2461 gccagccggg cggcgaaggg tattattacc agtggggctg gtatgaaggt ccggatgacg
     2521 cggcatggcg ggtgacgggg caccacacgg acagcggtga acagtaccgg ctggcgtggg
     2581 acctggcgca gcgcaggctg tgcgtgacgg acggactggg gcgcacacgt tatcaccagt
     2641 gggatgcgca gaatcaggtg acggcgtacc aggacgaagc cggacaggtg acgacattcc
     2701 gctggagcga cgaagaacga ctgttgctgg ggatgaccga ccctcagggc ggcaaatggc
     2761 gctatgtgta tgacctgcag gggcatatca cggagacgca cgacccgctg gggcgggtgg
     2821 agcaggcgca gtggcacccg gtgtggcacc agccggaaac ggaggtggac gcggcgggaa
     2881 acctgctggg gaggcgcgcg ggggaaaggg ccacggcaga aaacagcgtg gtgccgttca
     2941 accggctgat gtcttaccgt ggtgtgcact accgttatga cgaatttggc cgtgcggtgg
     3001 aaaaggaggg ccggagcgga acgcagagtt accgttatga tgcggaacac cggatggtgg
     3061 aagtgacgac agcgcggggg acataccggt atgtgtacga tgcactgggg aggcggacgg
     3121 aaaaacagca catcagccct gacggtaagc cgtacaaccg gacaaaattt ttatgggacg
     3181 gaatgcggct ggcgcaggag agcaggccgg agggaacagg cagcctgtac atctacaggg
     3241 accaggggag ttatgaaccg ctggcgcggg tggataaggc gggcaaagag ggcccgaacc
     3301 ggatactgta ttttcacacg gatgtgaacg gcgcgccgga agagatgacg gacagcgacg
     3361 ggaaaatcgt gtgggagacg gggtatcagg tctggggtaa cacgatacag gagaaggacc
     3421 acggcggggt ggagcagaac ctgcgctacc agggacagta cctcgacagg gaaacggggc
     3481 tgcactacaa tctgcacagg tactatgatc cggatgtcgg gcggttcatg gtaactgacc
     3541 cgatagggtt gagaggcggt ttaaatctct acagttatgc acctaatcct ctaaaatatg
     3601 ctgatccact tggattaact ccatgtgctg ttagtaacca aaaagcgaat cgtttattag
     3661 atagtagtga aaccaaagtt actgttaggt ctagaagtga cgctgaacaa ttgtttatgg
     3721 atagatattt aggtcataat tataaaaata tgacaggtga gtcaggcccc agcactaaga
     3781 acttaatgga atatcttact gaaaataaaa caaaagcagg ttcatatcat tgggatgata
     3841 taaaagatcc ttctgtaaca aaaccttcat atagagtgtc aggtcatgga ccaggaaatc
     3901 ctgatggtga tttaccacat ttacaagttc atcaacatgg tggaagtgtt agacatatat
     3961 ttttcccgtg ggagacataa taatgaacaa tctcaataca ttattaagtg gaaaggtttt
     4021 gctcaaagaa attagtccat taatgcctgg atatagaaca tggattgaaa tttcattgat
     4081 cgatgaatta aaaccatctt atccttttag attagatgaa tatgctatga tagggcattc
     4141 accttatgcc aatgaatgta ataaagatga agctaaattt aaactacgaa tatcatcatt
     4201 tttagcttct gacatagata atgaatacga tccatcatat gattatgttg gaaaatatga
     4261 aattatcgct tcacttaatg agttaaaaac taggttaagt actcttcatg taaacttgga
     4321 gcaatttata aattcatcgg aagatgatga gtatccatta taataagaca gaataaaaag
     4381 ctaggaaggt tttaaatctt cccagctttt ttaatattta actttcgttc agcacactaa
     4441 acatcccatc ccgcagttcc tgcacctcgt cactgtcagc gatgatggtc aggcccccct
     4501 ccgaaatctt caccgtgaca ccccgtccgg tatcaaatcc ggcctctttc agccagtcgc
     4561 ccttaaggtg cagactggga tgttgtgaat aataccgggc tataccggtt ttgcggtcct
     4621 ggtggcgggc tctgacatag ccgactttat agcggcgctg tgttttggaa atagtgactt
     4681 ctggcgtaat atcgtgttca gccatgatta actaccttga ttagttgctt gcggggagcg
     4741 cgctttcgta atcttgccct gctagcggat taacttcaca aacatcattt accaacgcct
     4801 acacttactc ctgtataccg ttcaggagca gtaatgaatc tacaacgatt tgatgacagc
     4861 accctaatcc gtatcttcgc ccttcatgag ttacatcgac tgaaagaaca tggcttaacg
     4921 cgcggggcgc ttctcgatta tcacagccgc tataaactcg tctttctggc ccattctcag
     4981 cccgagtacc gcaaacttgg cccgtttgtg gctgatattc atcagtggca aaatctggat
     5041 gatttttaca accagtaccg ccaacgcgta attgttttgc tttctcatcc cgccaacccg
     5101 cgcgatcaca ccaacgtttt gatgcacgtt cagggttatt ttcgcccgca tattgattcc
     5161 acagaacgcc agcagctggc tgcgcttatc gacagttatc gccgtggcga gcaaccactt
     5221 cttgcgccgc tgatgcgtat caaacactat atggcgcttt atcctgacgc ctggctttca
     5281 gggcagcgtt atttcgaact ttggccgcgt gtgattaact tgcgccattc aggagtttta
     5341 tgactaccca tctggcctgg tttcgccagg atttacgtct gcacgataat ctcgcactgg
     5401 ctgccgcctg ccgcaattcg tctgcacgcg tgctggcgct gtatatcgct acaccacgca
     5461 agtgggagac gcataacatg tcgccgcgtc aggctgaact tatcaatgct caactgaatg
     5521 ggctacaaat cgcgctggcg gaaaaaggta ttcctttatt gttccgtgaa gtggatgact
     5581 ttgtcgccag tgtcgaaata gttaaacagg tgtgcgcgga aaacagcgtt actcatcttt
     5641 tttataacta tcagtatgag gtgaatgagc gagcgcggga tgtggacgtt gaaagagcgc
     5701 tgcgtaacgt ggtgtgtgaa ggatttgatg acagcgtgat cctgccgccg ggggcggtga
     5761 tgaccggcaa tcacgagatg tacaaagtct ttacgccttt taagaatgcc tggctgaaac
     5821 ggctgcggga agggatgccg gagtgcgtcg ctgcaccaaa agttcgtagt agcggatcga
     5881 taaagcccgc gccatccatt acgctgaatt atcctcgtca gtctttcgat actgcgcatt
     5941 tcccggtgga agaaaaagcg gcgattgcgc aattacgcca gttttgcgag aacggtgctg
     6001 gagagtatga gcaacaacga gattttccgg cagtggaagg caccagccgt ttgtccgcca
     6061 gcttggcaac gggcgggtta tcgcctcgcc agtgtttgca tcgcttgttg gcggaacagc
     6121 cgcaggcgct ggacggtggg gccggtagtg tctggcttag tgagctgatc tggcgcgagt
     6181 tctaccgtca tctgatgacg tattacccct cgttgtgtaa acatcgtcca tttattgcct
     6241 ggacggatcg tgtgcagtgg caaagcaatc ccgcacattt acaggcctgg cagaaaggca
     6301 aaacgggata cccgattatt gatgccgcta tgcgtcagct taacagcact ggctggatgc
     6361 ataaccggct acggatgatt acagccagtt ttctggtgaa agatttgttg atcgactggc
     6421 gcgaaggcga gcgatatttc atgtcgcagc tgattgatgg tgatttggca gctaataacg
     6481 gtggctggca gtgggcggct tctacgggta ctgatgctgc gccgtatttt cgtattttca
     6541 atccgataac tcagggcgag aaatttgacc gtgagggcga gtttattcgt cgatggttac
     6601 cggagctgcg cgatgtacca gggaaagcgg tgcatgagcc gtggaagtgg gcgcagaaag
     6661 caggtgtgaa gctggattat ccgcaaccga tagtcgagca caaagaggcg agagtacaaa
     6721 cgttggcagc gtatgaggcg gcgcggaagg ggaaataaca gacgcgtcag gcaatcgagc
     6781 ccagatgccg gatgcggcgt gaacgcctaa tccagcctac aaatttgtgc aaattcaata
     6841 aattgcagga aatacgtagg cctgataaga cgcgtcaagc gtcgcatcag gcatcggtgc
     6901 tggcctatta tgactccagc gccagcgcgc ggtttctgaa tttcagcgcc tgatacagcc
     6961 aaatcatcag taccagtcct acacacgcca gtgcgcccca ggtaatttga tcaaatactt
     7021 caatatatgc attgatggag tagttgatcg ccccggaagc atcaaacgaa gcctgcgatg
     7081 tctgatcggc aatcacgccc gccagatagt tcgcgatcgc gccagaaagc agcatgtaga
     7141 tgccggttaa tacgccggtc acgcccggaa tttcaatgcg cgtaatttgc gacatggcaa
     7201 ccgggtcgat aaacagttcc gcaaagccca tcaccgcgag gcctaacacc atcagtggca
     7261 gagaagagtg accatacatt gcggaccagc gggcgcttaa ggtcagaatg cagaatccgg
     7321 cgctcatcag gccaaggcca agagcaaatt ttccccagat gcgcacggta cgattacctg
     7381 cgacgctttc tttaaccacc cacgccagga acacaccgca cagcataact gcgaaggcat
     7441 taatcgactg gaacatcgcg gtcggaacgg tataaccaaa catatcgcgg ttaacgaagc
     7501 ggtcgatata aaggctaatc gagctaccgc cctgttgtgc gaaggcccag aacaacatac
     7561 tgaagaaggt gagcgtcaca atcagcccca gctccttccg ctgtttctgg ttttctgctt
     7621 tgcgataaat ttttgccagt acacccaggc caataatagt cgcgacaatt aaggcgtata
     7681 ccgaccactc tttccagaac agcacggtaa tcagtgcagg cgttgccacc agcagaacca
     7741 gcagccatcc ccagttcggc aggagaaagt ttgtcgcacg cagtactttt ttgttaacgc
     7801 cgcgggtatg agtgaaatga cgattgccac ataagaaaat gaccagaccc gcgatcattc
     7861 caaccgccgc caggccaaag cccatcgccc agctgtactc ttcctgggcg taaccacagg
     7921 cgataggtgc gataatagac cccacgttac ccgccgcata catcagcgag aaaccgccat
     7981 cacgacgcgg atcggttggc tcatacagct cgccgagcag acagcttacg ttagatttaa
     8041 acaggccata gccgcagacg ataatcgcca gggacagata gaggaatgac ggatggatct
     8101 cactggcacc cagcaccaca tgaccgatcg ccatcaacaa cgcccccagc atcaccgcca
     8161 tgcgattgcc gagaacttta tccgccaaaa agccaccgag gattggcgtg acatacacca
     8221 gcgaacagta ggcgctaaat aactcgtagg cgtgagtatc gttgtatttt agttgattgg
     8281 tgagatagag aatcagcagg gcacgcatgc cgtaaaagct aaaatattcc cagatttgca
     8341 gcgcaacgac atagtaaata gcgcgcggtt gtgatgcgtg tttattcata ataattccac
     8401 aattgacggc aacgtcacgt aatagttgcg cggcgtgctt gtgtttcctt aaagttaaaa
     8461 ctttgataca gatctgatta tttatgcaat atgctgtctg attgcataaa tatacattag
     8521 ctgaagcgtg atgatataaa caatttgcgt cttttggcgt cgggtttgtt tcttaatatg
     8581 tcaaaagatt ggctatcgac gaacaggcga tgtaacgcta tgttaacggt gtagctatca
     8641 gaatacggaa ggcgcgaaga tgaaaaacac cgaactggaa caactgatta acgaaaaact
     8701 gaacagcgcg gcgattagtg attacgcgcc gaatggtttg caggtggaag gcaaagagac
     8761 ggtgcaaaaa attgttaccg gtgtcaccgc cagccaggct ttgctcgatg aggcagtgcg
     8821 tctgggcgcg gatgcggtca ttgtgcatca tggctacttc tggaaaggag agtctccggt
     8881 cattcgcgga atgaagcgta accgtttaaa aacgttgctg gcgaatgata tcaacctgta
     8941 tggctggcat ttgccgcttg acgcacatcc tgagctgggc aataacgcac agctggcggc
     9001 gttactgggg atcacggtca tgggcgaaat tgagccgttg gtgccgtggg gagagctgac
     9061 catgcctgtg ccgggaatgg aactagcctc ctggattgaa gcgcgtctgg gacgtaagcc
     9121 gttatggtgt ggcgataccg gacctgaggt ggtccagcgc gtcgcctggt gcacgggcgg
     9181 cgggcaaagt tttatcgata gcgccgcgcg ttttggcgtg gatgctttta ttactggcga
     9241 agtttctgaa cagaccattc attcagcccg cgagcaggga ttgcattttt atgctgcagg
     9301 tcaccatgcc actgaacgcg gtggtattcg cgcattgagc gagtggctga atgaaaatac
     9361 cgatcttgat gtgaccttta ttgatattcc taatcctgca taacgaataa tcagagggat
     9421 cgaaagtgca acgagcgcgt tgttatctga taggtgaaac ggcggtagtg ctggaactgg
     9481 aaccgccggt gacgctggct agccagaaac ggatctggcg actggcgcag cgtctggtgg
     9541 atatgccgaa tgtggttgaa gccattcccg gcatgaacaa tatcacggtg attttgcgta
     9601 atcctgagtc gctggcgctg gatgccatag agcgtttgca acgctggtgg gaggagagcg
     9661 aggccctgga acctgagtct cgctttattg aaattccggt ggtttacggt ggtgcaggcg
     9721 gaccggattt ggcggtggtc gcggcgtatt gcgggttgag cgaaaaacag gttgttgaat
     9781 tgcactcctc cgtggaatac gtggtctggt ttttaggttt tcaaccgggc ttcccgtatc
     9841 tcgggagttt gccggaacaa ctacacacgc cacggcgcgc tgaaccgcgc ttgctcgttc
     9901 cggcaggttc tgtcgggatc ggcgggccgc agactggtgt ttatccgctg gcaacgccgg
     9961 gtggctggca gttgattggt catacctcac tcagcctgtt tgatccggcg cgtgacgaac
    10021 ccatcttatt acgtccggga gacagcgtgc gatttgtacc gcagaaggag ggaatatgct
    10081 gaagattatt cgtgcgggca tgtacaccac tgtgcaggat ggcggtcgtc acggtttttg
    10141 ccagtcgggt atcagccact gtggcgcact ggatatgcct gcgctgcgca ttgctaacct
    10201 gctggtgggt aatgacgcca atgcccccgc gctggagatc acgctcggtc agttaacagt
    10261 tgagttcgaa actgatgggt ggtttgccct gacgggtgca ggttgcgaag cgcggttgga
    10321 tgataacgcc gtctggaccg gctggcgatt gccgatgaaa gcaggccagc gtttaacgct
    10381 taaacgcccg cagcacggga tgcgcagtta tctggcggtc gcgggtggta ttgatgttcc
    10441 gccggtaatg gggtcatgca gcaccgatct caaagtgggg attggcgggc tggaaggccg
    10501 tttactgaag gatggtgact gactcccgat tggcaaatcg aagcgtgatt ttatggaagc
    10561 gcagggcgtt aaacagctgc tgtggggcaa ccgcattcgc gccttgccgg ggccggaata
    10621 tcatgagttc gatcgcgcct cgcaggatgc attctggcgt tcgccctggc agcttagctc
    10681 gcaaagtaac cgaatgggct atcgtttaca ggggcaaatt ttaaaacgca tcaccgatcg
    10741 cgaactgtta tctcacggtt tgttaccggg cgtggtgcag gtgccgcata acgggcagcc
    10801 gattgtgttg atgaacgacg cacagaccac cggtggttat ccacgtattg cctgtatcat
    10861 tgaggctgat atgtaccatc tggcgcaaat tccgctcggt cagccgattc attttgtcca
    10921 gtgttcactg gaagaggcac tgaaagcgcg gcaagatcag caacgttatt ttgaacaatt
    10981 agcgtggcgg ctgcacaatg aaaattgacc tgaacgccga tctgggcgaa ggctgcgcca
    11041 gcgacgcaga gctattaacg ctggtttcct ctgccaatat tgcttgtgga tttcatgcag
    11101 gcgatgcgca aaccatgcag gcttgcgtgc gtgaagcaat aaaaaatggt gtcgcgattg
    11161 gcgctcaccc gagttttccc gacagggaaa attttggtcg cagcgccatg cagctgccgc
    11221 cggaaaccgt ttacgcccag acgctgtatc aaattggcgc gctggcaacg attgcccgtg
    11281 cgcaaggcgg cgtaatgcgt catgtcaaac cgcacggcat gttgtacaac caggcggcga
    11341 aagaagcaca actggcagac gccatcgcca gagcggtata cgcttgcgat ccggcattgg
    11401 ttctcgttgg gctggcagga agcgagctga ttcgtgcagg caagcaatat ggcctgacaa
    11461 cgcgcgagga agtgtttgcc gatcgcggtt atcaggctga cggctcgctg gtgccgcgaa
    11521 gtcagccagg cgcgttgatt gaaaacgaag aacaggcgtt ggcgcaaacg ctggagatgg
    11581 tgcaacacgg cagagtcaaa agtatcaccg gcgaatgggc aacggtcgcg gcgcaaacgg
    11641 tctgcctgca tggcgacggc gagcacgctc tggcgttcgc ccgccgatta cgagctacat
    11701 ttgccaaaaa ggggattgtt gtcgcagcat aaccccgctt aataaagaat gaaaaaagga
    11761 tatcaccatg cctgaaggcc cggagatccg ccgtgcagcg gataacctgg aggcggcgat
    11821 caaaggcaaa ccactaactg atgtctggtt tgccttcccg cagttaaaaa cttatcaatc
    11881 acaacttatc ggtcaacacg ttacccatgt ggaaacgcgt ggtaaggcgt tgttaactca
    11941 ttttcccaac ggtttaacgc tctacagcca taatcagctt tacggcgtct ggcgcgtggt
    12001 tgataccggc gaagagccgc agaccacgcg agtattgcgg gtaaaactgc aaacggctga
    12061 caaaaccatt ctgctttata gcgcctcgga tattgagatg ttgaggccgg aacaactgac
    12121 cacgcatccg tttttacaac gcgttggtcc cgatgtgctg gatccgaatc tgacgccgga
    12181 ggtggtaaag gaacggttat tgtcgccgcg ctttcgtaac cgtcagtttg caggattact
    12241 gctcgatcag gcgtttctgg ctgggcttgg caattatttg cgggtggaga tcctctggca
    12301 ggttgggttg actggaaatc ataaagcgaa agatctcaat gcggcgcaac tggatgcact
    12361 cgcacacgcg ttactggaga ttcctcggtt ttcttacgct acgcgggggc aggtggatga
    12421 gaataagcat catggggcgc tgtttcgctt taaggttttt catcgtgatg gcgaactgtg
    12481 cgaacgctgt ggcggcatca ttgagaaaac cacgttgtcc tcgcgcccgt tttactggtg
    12541 tccaggctgc cagcactagg ccgaccgctt cggcgcatag gttgaaataa accgcgcaat
    12601 ggctggcccc gtcagcaaaa tactgaacag gcgcagggtt tgcatcgcca taataagcgc
    12661 catatcggca ttgctccctg cggcgatgac cgccacggta tcaagcccgc cgggactggt
    12721 ggcgaggtag gcagtcataa aatcaatatg cataaaccgg gtcagtcccc acgccatacc
    12781 tgcacaaata gccagcagag caaaaatcga cagcaggatt tgcggtagcg ggcgtagtgc
    12841 ccgcagtaag atttgtttat cgaaaccaag accaatccgc cagccaattg ccatatacgc
    12901 catcgccagc agccattccg gtagttcgat ggtgatgagc tgaccagact ggagcactgc
    12961 gcccgccagc atcgggatga gcatcgtgcc ggaaggaaga cgcagcatgc atcccgccgt
    13021 accggcaacg actgcgagca aaatcgtaag caggagatta atgctcactg gcgggaacca
    13081 gacgatttgc tggttaaccg cttcagcgtt atcacccagc atcatgcggg tgaccagaac
    13141 agcggccccc gcgacgaaca gcactcgcag atattgcata aacgccacca ggcgaatatc
    13201 tgcgccgtaa tcttgcgcca tagcgaccat tgccgctgcg ccgcctggag aagaacccca
    13261 ggcaccggta tttccaggca gtgagctata gcgcaccaat aaccaaccca cgatggcgct
    13321 ggaaagcagc gtcaccagta aaatagcaag cacgatcggc cagttggcgg ccagcgtggt
    13381 gagaatcgaa ccagtgaggt tttgcgcaat catgcagcca agaatggcct gcgcggcgag
    13441 aaaagcggag cggggaagtt gcagagtaat tccgcgcata ctgaagatga tcccggcgat
    13501 catcggccca agtaatagcg ccgccgggag atgtaccgcg aggaaaccaa tagaaaggag
    13561 aagtgatagc acacataaca ttccccactg caaaactggc atccttcgct ccctcattat
    13621 tttctctttg gtgtatcgat acagcataaa gaaagccggg ctaggaaaaa ataacagcaa
    13681 tcaaggaaaa ggggaaaatc agcaattttc tgaaagagat gccctttccg gctgcaaagg
    13741 gcatcatggc aaatcagtta tagttaattt taaaaagcac cactgtatcg aagggcccag
    13801 cctcgatggg ggtacctgca atggacgaaa gttcagctgt ataggttttt tcgtagacca
    13861 acatgtcgct ggtgaaatcg gcgtattcag catatttatt gaaggtataa ggcgaggcgt
    13921 tttcatccag caaacgtagc ttcagaccat taccaatcaa caatgcttta tcttcttcaa
    13981 ccaacgtttc ttcggtataa aaggaggaac tgactttaaa tccgtcggtg cattggtcat
    14041 tttgtgattt tgtcgtctta atactgaatg ttttcgacat tgtgtggcga cgaatatcca
    14101 gcaaattaaa tttaccaaaa tcaatcatct gtgtttccgg tgaaatggaa aaattgacgc
    14161 tgcaatccag cacgcgaatg ttttccagac cagtaatatg atatttcagg ttttgggcag
    14221 tagggtcttc gttaacgcta ccggcaccgt caaattgcac gacgatatag tcgctaagcg
    14281 tactttgata atcatgcggc ggcatttctc gtattttgac gtataaccgc atgcgagcga
    14341 gaaaggttcg cgacatgtga atattagcgg gatcacccga gcaaattttg ttctgccatc
    14401 ccatcagggg tataaatgtc aatgttctta ctgtcgatac actggttagt gtcgatgcgg
    14461 ctttttccct ggttggcgtc ataatcaacg ccattgtaag ttactccgag ttgataataa
    14521 cgatcctgca cccccgggta ggggtttacc catacgtaga catgttcgct ttcaaagttg
    14581 ccgttggtat tgttgtcaca atagacggga attttaatat cgtcagattc ccagatctta
    14641 ttttaatatc gtcagattcc cagatcttat cgccgagttt ggcattgcct ggtacagcaa
    14701 aaggttgaat cgcttctgat ttttctaccg agccaccgga tgaaccaaag tagcagttca
    14761 gggcaagcgc agacttcaac gacagaaata acagacagat gacgagcaac actcccttgc
    14821 cagcactcat ttcttcgcct ccacggcttg taaatggcat tgtccgctgc agttgagcgt
    14881 cgtcatgcgt aacgcgccgt aatcatccat ataaccgaga taaaactggc tgccgctata
    14941 atttccggtg tttgtgttga ccgtactaaa aggcgcaacc atcacggttt tgaagccggg
    15001 gagaacgcct ttttcatttc gtcccaggta ggcaatggtc agataatacg cggtaggatt
    15061 tttcagtgtc agttggttgc cctgctggct gacctgtaac tgcagttcga ccttttctcc
    15121 tgctttcttg cgtaatgcag ccggtcgcca gaacaccttg atacgactct gaatggcgac
    15181 ttgcagaacg gcatggtcgc tgtttttttc cggggcggga ggaatttcac gcatattgta
    15241 gaaaaatagc gtttcgcgat cgcccggtaa ttttgtggtc gaggcttgct ttactatccg
    15301 tacctgtgtt gttgctttcg gttccagacg ttgtatcggc ggcagggcga ccagaagatc
    15361 atcgcttttc tccccttttt cattttcgag ccaggaataa gcgaggtagg ggagtttatc
    15421 gctacgatta tccacccgca gactggtagc tttatcatta gcgttaaaca caatacgagt
    15481 gcgatcgggt tgtagtgcag cgtggctgca cagactggca accaacaaca aaaggggtag
    15541 tcctttcata aatgtcattt tcatttcctt aattaatgac aaggtaaaat caggcgattt
    15601 gcggtgtctt ccatatgttc aggaagatgg agcgagcaat gctggctatc accccagacc
    15661 acggtaaact tttgattttc agcgactccg cttaaccagg catgtccttc ctcgccaacc
    15721 atccccacgc taatgccgct gtcatcctgg cggatatccg ctccgagggg aggaaactga
    15781 ccgctggcgt tgcgaatgat gacgttaacg tctttaccgg aacgggaagc cagtgatttg
    15841 taacctatcg cgccttcaat ccacgtttct ttgataacgt tttctgcaac tgttacgccg
    15901 tcgggtaagt cattcatgtt caccgccacg gtggaaggct ggtaactgga aatcaacggc
    15961 accaccgcaa tgccaaaatg gttggtgtag tcgagattgc cctgaaccgg aatatctgcc
    16021 acgccatcgg tgctgaccat caggcgtggt tcattggtgg agctgcggcg atgaaacgct
    16081 gcaccatatt gggttgcggt gaaagaaccg ctccagctgc tgctgacgga actgtaatca
    16141 ttggcggcat aggtaccaga aatatcccac tcacccgctg aactcaggtg ctgatagtta
    16201 ccgctcacct gggctccatt gtcaggacgg tcggattgca gtccggcaga catgccccag
    16261 ctattacgtt catccagcgt atcgttccac gacatgcggt gtgtggtgct gtggctactg
    16321 ttttgcatgt catagccaac ccgaccaccg ttaccgaagg ggagcgaaat cgacaggtaa
    16381 atctggttgt cgcgatcttt atcttcgtaa tgggttgtat tgaatgacgt agaaatagag
    16441 atatctctcc agtcaccaat atcaacatta aaacctgccg tgatgttggc ggtcgtggag
    16501 gcgtctgcat tccaccaggt ttgatgtagc aggttggcgt aaagattgag gtttagtggg
    16561 gtaatcggtt ggcccacaga taagctgatc gtctgttttt cgtcctgcgc atcgctgtcg
    16621 ttgtatttgt gatccagata gttggcgtag ctgtgaaaat gacgatcgga gaaacgataa
    16681 gcggcgaggg aaatcgtgct gttagtggca tccacttgtt tgctgtaatt aaaacggtag
    16741 cttaagcccc gctcgtcctg ctgagtatca aaatggctac tggcccacgt gacatcaaac
    16801 gacagcgcac caagccacag catattttgc ccaataccca ttgcggcaga atggtagtca
    16861 tcaccagaaa gcagcaggcc gctgtacagc gaggtgtttg acagcatccc ccaggaaact
    16921 tcattgctaa aaaaggtttc attttcagtt tgatgtgaca tggagggccg tggctgaccc
    16981 gcggccagtt tatagcgaac ctgtccctga cgagtcagga agggcgtcga tgctgctgaa
    17041 acctggaaat tgttcacccg accatcttct tccgtcactt tgacatccag tgtgccctga
    17101 acagactgat taaggtcgtc aatgataaat gggcctggtg ggactttttt ctggtaaatg
    17161 acgcggcctg attgactgat cgtcaccgtg gcattggtct gtgcaatacc gctaatttgt
    17221 ggggcgtagc cgcgtagttc ccatggcaac attcgatcgt cacttgccag tgccgcgccg
    17281 gtataagaaa aaccgtcgaa aatattggga ctaaaatccg tttcgccgag ggttaatttt
    17341 gaacctaatt gcggtaacgg gcgaaaaaga taggtacgtg atatttcgcc tgactgttca
    17401 tggttatcat cgctatcagt atgattcaac tgataatcac tgcgtaagcg ccatgccccc
    17461 gtgttgattc cggctgttcc ataggcgtta agattagtgc tgctgctgcc atcctgtggg
    17521 cggtagctgc tggcaaacaa gttgtaatcc atcaggacac cggcgacacc ttctttccat
    17581 gtggagggtg gggtccagtt ttctgagtgc cacgccagcc aggcttgcgg aatactaata
    17641 ttcagttgct gattggcttg atcgaaattg aagagcattt caggtcggga gctaaaatcg
    17701 acgcactgat ttatcaatgg taacgactga cggacttcag gttttaagcc aaatttatcg
    17761 accagtaaat cattgatgca cggaatggtt ttatcgtcat ttttgtgcca gttaatcttt
    17821 tgctcgttac tgatttggtt attatttacc gcaacgctaa caaaatactc accgggagca
    17881 atgactcctt tttcctttaa taatgaaata tcaatgcggt cgcgcattga tttatcgaga
    17941 acattcagat tgaattcgac cgccaatgcg caaggcatcg ccacgaccag gcaggataca
    18001 aaagagagtc gataaatatt cacggtgtcc atacctgata aatattttat gaaaggcggc
    18061 gatagcgccg ccaaagtaat acttatttat aatccagcac gtaggttgca ttcgccgtaa
    18121 ctgcgcctgg cgttacaggt gtagcctgat caatttgttc catccaggca aagaaattca
    18181 gcgtttgttc gctggaggtc ggagccaggt cgatatctgg agtagcggtg cctaaaacga
    18241 tattgctttg atctttattc attaaacgta cgccgacgcc ggtggcttca cccgtgctgg
    18301 tgttgttgag aatcggatct gcgccggtgg ttttcgctga gctatcaaag gtgactgcaa
    18361 ctttagaaat cttgccaccg gaaccgttgc tggagttttc cagatcacag ttgactaaga
    18421 gcaggtcgac agcaacggca tcgctgtgat gtgactgatt aatgtggctg gtggtcacct
    18481 gaccgagttc aacctcttta tcaatatcat cctgatgtat ttcacacggt gcttcaataa
    18541 cttcaccttt aaagtgaatt tcaccggatc cttcatcagc cgcataaaca ggtgctgtga
    18601 acagcagaga tacggccagt gcggccaatg ttttttgtcc tttaaacata actgagtcct
    18661 ttaacagaaa agaaaaaagt atatcggtgc aagaatatct tatttgattg tcgaatgttt
    18721 t
//
LOCUS       DAZSNO010000068        18701 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZSNO010000068 DAZSNO010000000
VERSION     DAZSNO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18701
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.068"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            137..586
                     /gene="alaE"
                     /locus_tag="ACHWN2_002266"
     CDS             137..586
                     /gene="alaE"
                     /locus_tag="ACHWN2_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR8931785.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(623..967)
                     /locus_tag="ACHWN2_002267"
     CDS             complement(623..967)
                     /locus_tag="ACHWN2_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR8931786.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1119..1448
                     /locus_tag="ACHWN2_002268"
                     /pseudo
     CDS             1119..1448
                     /locus_tag="ACHWN2_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            1696..1941
                     /gene="nrdH"
                     /locus_tag="ACHWN2_002269"
     CDS             1696..1941
                     /gene="nrdH"
                     /locus_tag="ACHWN2_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR8931787.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1938..2348
                     /gene="nrdI"
                     /locus_tag="ACHWN2_002270"
     CDS             1938..2348
                     /gene="nrdI"
                     /locus_tag="ACHWN2_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFR8931788.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2321..4465
                     /gene="nrdE"
                     /locus_tag="ACHWN2_002271"
     CDS             2321..4465
                     /gene="nrdE"
                     /locus_tag="ACHWN2_002271"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR8931789.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4475..5434
                     /gene="nrdF"
                     /locus_tag="ACHWN2_002272"
     CDS             4475..5434
                     /gene="nrdF"
                     /locus_tag="ACHWN2_002272"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR8931790.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5789..6991
                     /gene="proV"
                     /locus_tag="ACHWN2_002273"
     CDS             5789..6991
                     /gene="proV"
                     /locus_tag="ACHWN2_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR8931791.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6984..8048
                     /gene="proW"
                     /locus_tag="ACHWN2_002274"
     CDS             6984..8048
                     /gene="proW"
                     /locus_tag="ACHWN2_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR8931792.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8106..9098
                     /gene="proX"
                     /locus_tag="ACHWN2_002275"
     CDS             8106..9098
                     /gene="proX"
                     /locus_tag="ACHWN2_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR8931793.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9290..10469
                     /locus_tag="ACHWN2_002276"
                     /pseudo
     CDS             9290..10469
                     /locus_tag="ACHWN2_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            10593..11330
                     /gene="ygaZ"
                     /locus_tag="ACHWN2_002277"
     CDS             10593..11330
                     /gene="ygaZ"
                     /locus_tag="ACHWN2_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR8931794.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11320..11655
                     /gene="ygaH"
                     /locus_tag="ACHWN2_002278"
     CDS             11320..11655
                     /gene="ygaH"
                     /locus_tag="ACHWN2_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR8931795.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11746..12276
                     /gene="emrR"
                     /locus_tag="ACHWN2_002279"
     CDS             11746..12276
                     /gene="emrR"
                     /locus_tag="ACHWN2_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR8931796.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12403..13575
                     /gene="emrA"
                     /locus_tag="ACHWN2_002280"
                     /pseudo
     CDS             12403..13575
                     /gene="emrA"
                     /locus_tag="ACHWN2_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            13592..15130
                     /gene="emrB"
                     /locus_tag="ACHWN2_002281"
     CDS             13592..15130
                     /gene="emrB"
                     /locus_tag="ACHWN2_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR8931797.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15194..15709)
                     /gene="luxS"
                     /locus_tag="ACHWN2_002282"
     CDS             complement(15194..15709)
                     /gene="luxS"
                     /locus_tag="ACHWN2_002282"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR8931798.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(15859..17415)
                     /gene="gshA"
                     /locus_tag="ACHWN2_002283"
     CDS             complement(15859..17415)
                     /gene="gshA"
                     /locus_tag="ACHWN2_002283"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR8931799.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(17488..17916)
                     /locus_tag="ACHWN2_002284"
     CDS             complement(17488..17916)
                     /locus_tag="ACHWN2_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR8931800.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(17913..18479)
                     /gene="yqaB"
                     /locus_tag="ACHWN2_002285"
     CDS             complement(17913..18479)
                     /gene="yqaB"
                     /locus_tag="ACHWN2_002285"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR8931801.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4435 a         4840 c         4742 g         4684 t
ORIGIN      
        1 taactaaata aaaatttttc actaattgat tagtcatagg cagcgatata cgctatgcaa
       61 aaatgcagat ggcaatgaga tccactgctt tcatctccat taacatccca ttacgctttt
      121 attaaggagc attagcatgt tctcaccgca gtcacgcttg cgtcatgcag ttgcagatac
      181 gttcgcgatg gttgtttact gttctgtcgt gaacatgtgt attgaagttt tcctctccgg
      241 aatgagcttc gaacagtctt tttattccag attggtagcg attccggtga acatcttaat
      301 tgcatggcca tacggtatgt accgcgatct gtttatgcgc gcggcacgca aagttagccc
      361 gtcgggctgg ataaaaaatc tggctgatat cctggcttat gtgacgttcc agtcaccggt
      421 gtatgtggcg atcttgttag tggtgggcgc agactggcat cagattatgg cggcggtcag
      481 ttcaaacatc gttgtttcga tgttgatggg ggcggtttat ggctacttcc tcgattattg
      541 ccgccgactg tttaaagtca gccgttacca gcaggtaaaa gcctgactct tcttttcgcg
      601 actggcatcg ccagtcgcgt ctttaactgg cttcgccaaa taaaccattc aaataacgtt
      661 caagcgcaac acgcgaacta aagccatgcg ggataccgta atgctcgtgt cgctcgcctg
      721 ctaaatagag cggaaattgc tggtaatgat cgcaagtttt aatatcggaa gcgggttcag
      781 ccagtgttga actgcgttct tttaatgttg gatgaattac cagagcggta cgtcccattc
      841 gcgcttcgcg attaacatag acataatttt ccccacggcg gtaaccatac gctttctggg
      901 ttaccacatc gacagtaaaa ccgacttttt caagtacgcg cgccacctcg tctggtcgta
      961 aatacatatt tgatcctcgt tattttcaac cgcgggccta ccttacctga ttgcgcattc
     1021 acaacgcttt cagaaaagtc cataaagccg cgatgaacct cagttaagaa aattatggtc
     1081 tacactgaaa attacatcga attctaatgg gggatcatat gtttaaccga ccgaaccgca
     1141 acgacgttga tgatggcgtg caggatatcc agaatgatgt caatcaatta gctgacagcc
     1201 tcgaatctgt attgaagtcc tggggcagcg acgccaaagg ggaagctgaa gccgcacgca
     1261 gtaaagctca ggcgttgctg aaagaaacca gggcgcgaat gcatggtcgt actcgcgtcc
     1321 agcaagccgc gcgcgatgcc gttggctgag cggattcttt tgttcgtgaa agaccctggt
     1381 gtagcgtggg tacagcagct gcggtaggta tttttatcgg cgcactgtta agcatgcgca
     1441 aatcgtagtg caaaaatgat aataaatacg cgtctttgac cccgaagcct gtcttcgggg
     1501 tttctttttg cctggtgaat cacaaaaatc cccctccccc gtcacgctca tatccagggt
     1561 aatttcgatc actatttgct atatattgtg tagttgaatc ttttttcaac tacatctagt
     1621 atctctgtat caacagagag acaacccgac gcgtatcatc gcgccgtatc ttcattttaa
     1681 acggaaatac gaatcatgcg cataactatt tatactcgta acgattgcgt tcagtgccac
     1741 gccaccaaac gggcgatgga aaaccggggc tttgattttg aaatgattaa tgtcgatcgc
     1801 gttcctgaag cagcggaagc gttgcgtgct cagggctttc gtcagttgcc ggtagtgatt
     1861 gctggcgatc ttagctggtc tggtttccgt ccggacatga ttaaccgtct gcatccagcg
     1921 ccacacgcgg ccagtgcatg agccagctcg tctacttctc cagcagctcc gaaaacacgc
     1981 agcgttttat cgaacgttta ggtctgcccg cggtgcgcat cccgctcaat gagcgggaac
     2041 ggattcaggt agacgagcct tacatcctga tcgtgccctc ttacggcggc ggcggtacgg
     2101 ctggcgcggt gccacgacag gtaattcgct ttttaaacga cgagcacaac cgggcgttgc
     2161 ttcgcggcgt tattgcttct ggtaatcgca actttggtga ggcgtatggc cgcgccggag
     2221 atgtgattgc ccggaaatgc ggcgtgccgt ggctgtaccg ttttgaactc atgggtacgc
     2281 aaagcgatat cgaaaacgtt cgtaaaggag taaccgaatt ttggcaacga caaccgcaga
     2341 atgcctgacg caggaaacga tggattacca cgcgctgaat gcgatgctta atctttacga
     2401 tagcgcaggt cgcattcagt tcgataaaga ccgccaggcc gttgacgcct ttattgcgac
     2461 gcatgtgcgt ccgaacagtg tgaccttcag tagccagcag cagcgcctga actggctggt
     2521 caacgaaggt tactatgatg aaagcgttct taatcgctac tctcgcgact ttgtcattac
     2581 gctgtttgcc cacgcacaca ccagcggttt tcgtttccag acattcctcg gggcatggaa
     2641 gttttacacc agctatacgt tgaagacatt cgacggtaaa cgttatctgg aagattttgc
     2701 cgatcgagtc acgatggtgg cgctgacgct ggcacaaggc gatgagacgc tggcgttgca
     2761 actgaccgat gaaatgctgt caggacgctt tcagccagcc acgccaacat tcctcaactg
     2821 cggtaagcag cagcgcggcg aactggtttc ctgttttttg ctgcgtattg aagacaatat
     2881 ggagtcgatt ggtcgggcgg taaattccgc actgcagctg tcgaaacgcg gcggcggcgt
     2941 agcatttttg ctgtcgaatc tgcgggaagc gggcgcgcca attaaacgta ttgaaaatca
     3001 atcttctggc gtaattccgg tgatgaaaat gttggaagac gcattttcct atgccaacca
     3061 actcggcgcg cgtcaggggg caggtgcagt ctatttacat gctcatcatc ccgatattct
     3121 gcgttttctc gacacgaaac gggaaaatgc cgacgaaaaa atccgcatta aaacactgtc
     3181 gctgggcgtg gtgatcccgg atatcacttt ccatctggca aaagagaatg cgcagatggc
     3241 gctgttttcg ccctatgacg ttgagcgagt ttatggcaag ccgtttgccg atatcgccat
     3301 tagcgaacac tatgacgaac tggttgccga tgaacgcatc cgcaaaaaat acctcaacgc
     3361 ccgtgatttc ttccagcgac tggcagaaat ccagtttgag tccggctatc cctacatcat
     3421 gtatgaagac acagtaaacc gtgctaaccc tatcgccggg cgcataaata tgagtaatct
     3481 ctgctcagaa attttgcagg ttaacagcgc ctcagagtat gacgagaatc tcgactatgc
     3541 ccgcacaggc catgatattt cctgcaattt aggttcgttg aatattgcgc acaccatgga
     3601 ttcccccgat ttttcccgca cggtagagac tgccgtgcgc ggtttaacgg cggtgtcaga
     3661 tatgagtcat atccgcagcg tgccgtccat cgaagccgga aatgccgcct cgcacgccat
     3721 cggactgggg cagatgaatt tacatggtta tctggcgagg gaaggcatcg cttatggttc
     3781 gccggaagcc ctggatttca ccaatctcta tttctatacc atcacctggc acgcactgcg
     3841 tacctcgatg ttgctggcgc gcgaacgcgg tgaaaccttc gccgggttca aacagtcacg
     3901 ctatgccagt ggtgaatatt tcagccaata tctgcaaggt aactggcagc cgaaaacggc
     3961 gaaagttggc gaactgtttg cccgcagctg tattacgtta cctacccgtg agatgtgggc
     4021 gcaactgcgc gacgacgtga tgcgctacgg catatacaac cagaatctcc aggcggtgcc
     4081 gccaaccggg tctatctctt atatcaacca tgctacgtcg agtattcatc cgattgtggc
     4141 gaaagtagag atacgcaaag agggcaaaac aggacgcgtt tactaccctg ccccgtttat
     4201 gactaacgaa aatctggcgc tgtatcagga cgcttacgaa attggcgcag aaaagatcat
     4261 cgacacctac gcggaagcga ctcgccatgt cgatcagggg ctgtcgctga cgctgttttt
     4321 ccccgatacc gccaccactc gcgatatcaa caaagcgcag atttacgcct ggcgcaaagg
     4381 tatcaaaacg ctctattaca tccgcctgcg tcagatggcg ctggaaggca ctgaaattga
     4441 aggctgcgtc tcctgtgcac tttaaggaat atctatgaaa ctctcacgta tcagcgccat
     4501 caactggaac aagatatctg acgataaaga tctggaggtg tggaatcgcc tgaccagcaa
     4561 tttctggcta ccagaaaagg tgccgctgtc gaacgatatt cccgcctggc agacattaac
     4621 tgtcgtagaa caacaactga cgatgcgcgt ttttactggc ctgacgctgc tcgacacgct
     4681 gcaaaatgtt atcggcgcgc cttcgctgat gcccgatgcg ctcacgcctc atgaagaggc
     4741 ggtattatcg aatatcagct ttatggaagc ggttcatgcc cgctcttaca gttcgatttt
     4801 ctcgacgctg tgccagacca aagatgtcga tgccgcctac gcctggagtg aagaaaacgc
     4861 accgttgcag cgaaaagctc agattattca gcaacattat cgcggtgatg atccgctgaa
     4921 aaagaaaatc gccagtgtgt ttcttgaatc ttttttgttc tattccggtt tctggctgcc
     4981 gatgtatttt tccagccgcg gaaagctgac caataccgcg gacctgatcc gtctgattat
     5041 ccgcgatgaa gcagtccacg gttactacat aggctataaa tatcagaaaa acatggaaaa
     5101 gatatctctg ggacaacgtg aagagttgaa gagtttcgcc ttcgatttgt tgctggaact
     5161 ctacgacaac gagttgcaat acaccgatga gctgtacgcc gaaaccccgt gggctgacga
     5221 tgtgaaagcg tttctctgtt acaacaccaa taaggctttg atgaatctgg gctacgaacc
     5281 gttatttccc gcagaaatgg cggaagtgaa tccggcaatc ctcgccgcgc tttcgccgaa
     5341 tgccgatgaa aatcacgatt tcttttccgg ttcaggctcc tcttatgtga tggggaaagc
     5401 ggttgaaaca gaagatgaag actggaattt ctgagggtgt tattttcaaa aatatcacta
     5461 cccgcagcag ggaaataatt cccgccaaat agctttttat cacgcaaata ttctgtagtg
     5521 atctacactg atactctgtt gcattattcg cctgaaacta caatattcag gcgttttttc
     5581 gctatctatg acaaaaaata tcaacttttc tcgatttgct ctcagccctt atatcacgga
     5641 aaattccggc gatttgctcg catcaatatt catgccacat ttgccctcag gggttgcctc
     5701 agattctcag tatgttaggg tagaaaaaag tgactattcc cattgggtaa tatatcgaca
     5761 taaacaaata aagaaatcat tctattgcat ggccattaaa ttaaaaatta aaaatcttta
     5821 taaaatattt ggtgagcatc cacagcgagc gttcaaatat atcgaacaag gactttcaaa
     5881 agaacaaatt ctggaaaaaa ctgggctatc gcttggcgta aaagacgcca gtctggccat
     5941 tgaagaaggc gagatatttg tcatcatggg attatccggc tcgggtaaat ccacaatggt
     6001 acgccttctc aatcgcctga ttgaacccac ccgcgggcaa gtgctgattg atggtgtgga
     6061 tattgccaaa atatccgacg ccgaactccg tgaggtgcgc agaaaaaaga ttgcgatggt
     6121 cttccagtcc tttgccttaa tgccgcatat gaccgtgctg gacaatactg cgttcggtat
     6181 ggaattggcc ggaattaatg ccgaagaacg ccgggaaaaa gcccttgatg cactgcgtca
     6241 ggtcgggctg gaaaattatg cccacagcta cccggatgaa ctctctggcg ggatgcgtca
     6301 acgtgtggga ttagcccgcg cgttagcgat taatccggat atattattaa tggacgaagc
     6361 cttctcggcg ctcgatccat taattcgcac cgagatgcag gatgagctgg taaaattaca
     6421 ggcgaaacat cagcgcacca ttgtctttat ttcccacgat cttgatgaag ccatgcgtat
     6481 tggcgaccga attgccatta tgcaaaatgg tgaagtggta caggtcggca caccggatga
     6541 aattctcaat aatccggcga atgattatgt ccgtaccttc ttccgtggcg ttgatattag
     6601 tcaggtgttc agtgcgaaag atattgcccg ccggacaccg aatggcttaa ttcgtaaaac
     6661 tcctggcttc ggcccacgtt cggcactgaa attattgcag gatgaagatc gcgaatatgg
     6721 ctacgttatc gaacgcggta ataagtttgt cggcgcagtc tccatcgatt cgcttaaaac
     6781 cgcgttaacg cagcagcaag gtcttgatgc ggcgctgatt gatgcgccgt tagcagtcga
     6841 tgcacaaacg cctcttagcg agttgctctc tcatgtcgga caggcaccct gtgcggtgcc
     6901 cgtggtcgac gaggaccaac agtatgtcgg catcatttcg aaaggaatgc tgctgcgcgc
     6961 tttagatcgt gagggggtaa ataatggctg atcaaaataa tccgtgggat accacgccag
     7021 cggcggacag tgccgcgcaa tccgcagacg cctggggtac accgacgact gcaccggctg
     7081 acggcggtgg tgctgactgg ctgaccagta cgcctgcgcc aaacgtcgag cattttaata
     7141 ttctcgatcc gttccataaa acgctgatcc cgctcgacag ttgggtcact gaagggatcg
     7201 actgggttgt tacccatttc cgtcccgtct tccagggcgt gcgcgttccg gttgattata
     7261 tcctcaacgg tttccagcaa ttgctgctgg gtatgccagc gccagtggcg attatcgttt
     7321 tcgctctcat cacctggcag atttccgggg tcggaatggg cgtggcgacg ctggtctcgc
     7381 tgattgccat cggcgcaatc ggtgcctggt cgcaggcaat ggtgactctg gcgctggtgt
     7441 taactgccct gctgttctgt atcgtcatcg gtttgccgtt ggggatatgg ctggcgagaa
     7501 gtccgcgagc ggcgaaaatt attcgtccac tgcttgatgc catgcagacc acgccagcgt
     7561 ttgtttatct ggtgccaatc gtcatgctat ttggtatcgg taacgtgccg ggcgtggtgg
     7621 tgacgatcat ctttgcgctg ccgccgatta tccgtctgac cattctgggg attaaccagg
     7681 ttccggcgga tctgattgaa gcctcgcgct cattcggtgc cagcccgcgc cagatgctgt
     7741 tcaaagttca gttaccgctg gcgatgccga ccattatggc gggcgttaac cagacgctga
     7801 tgctggccct ttctatggtg gtcattgcct cgatgattgc cgtcggcggg ctgggtcaga
     7861 tggtacttcg cggtatcggt cgtctggata tggggcttgc caccgttggc ggcgtcggga
     7921 ttgtgatcct cgccattatc ctcgatcgcc tgacgcaggc cgttgggcgc gactcacgca
     7981 gtcgcggcaa ccgtcgctgg tacaccactg gccctgttgg tctgctgacc cgcccattca
     8041 ttaagtaact ctgcacttgc ccggtgacgc cgggcattat caccctgcca aaaaaaggaa
     8101 taacaatgcg acatagcgta ctttttgcga cagcgtttgc cacgcttatc tctacacaaa
     8161 cttttgctgc cgatctgccg ggcaaaggca ttactgttaa tccagttcag agcaccatca
     8221 ctgaagaaac cttccagacg ctgctggtca gtcgtgcgct ggagaaatta ggttataccg
     8281 tcaacaaacc cagcgaagtg gattacaacg ttggctacac ctcgcttgct tccggcgatg
     8341 caaccttcac cgccgtgaac tggacgccac tccatgacaa catgtacgaa gctgccggtg
     8401 gcgataagaa attttatcgt gaaggggtat ttgttaacgg cgcagcacag ggttacctga
     8461 tcgataagaa aaccgccgac caatacaaaa tcaccaacat cgcacaactg aaagatccga
     8521 agatcgccaa actgttcgat accaacggcg acggtaaagc ggatttaacc ggttgtaacc
     8581 ctggctgggg ctgcgaaggt gcgatcaacc accagcttgc cgcgtatgaa ctgaccaaca
     8641 ccgtgacgca taatcagggg aactacgcag cgatgatggc cgacaccatc agtcgctaca
     8701 aagagggcaa accggtgttt tattacacct ggacgccgta ctgggtgagt aatgagctga
     8761 agccagggaa agatgtggtc tggttgcagg tgccgttctc cgcactgccg ggcgataaaa
     8821 atgccgatac caaactgccg aatggcgcga attacggctt cccggtcagc accatgcata
     8881 tcgttgccaa caaagcctgg accgagaaaa atccggcggc agccaaactg tttgccatta
     8941 tgcagttgcc agtggccgat attaacgccc agaacgccat tatgcatgac ggcaaagcct
     9001 cagaaggcga tattcagggc cacgttgatg gctggatcaa agcccaccag cagcagttcg
     9061 atggctgggt gaatgaggcg ctggcagcgc agaagtaatt tttattcggg cggataaggc
     9121 gttttcgcct catccgccgc tctgtacaca atgcctgatg cgacgctgac gcgtcctatc
     9181 atgcctacaa accacatcgc acatttcaac aagctatctt tcatcccata ttcatcaaca
     9241 tccgctatta ttgatttcca gcttaatcat cacctgatga acaaaaataa tgactaaacc
     9301 taatcatgag cttagcccgg cgctgatcgt gctgatgtct atcgccaccg gtctggcggt
     9361 agccagtaac tattacgccc agccattgct cgacaccatc gcgcgtaact tttccctttc
     9421 cgccagttcg gcaggcttta ttgttaccgc cgcgcagttg ggctatgccg caggtctact
     9481 gtttcttgtt cccctcggtg atatgtttga acgccgccgc ctgattgtct cgatgacctt
     9541 actggcagcg ggcggtatgt taatcactgc cagcagtcag tcactggcga tgatgatcct
     9601 cggtacggca ttaaccggtt tattctcagt cgtggcacaa attctggttc cgctggcagc
     9661 gacgctggct tcaccggata aacgcggcaa agtggttggc accattatga gcggtctgct
     9721 gttggggata ttgctggcac gaaccgttgc cgggttactg gcaaacctcg gcggctggcg
     9781 caccgtcttt tgggttgctt cggtgttaat ggcactgatg gcgctggcat tatggcgtgg
     9841 tctgccacaa atgaaatcag aaacccacct caactaccca cagttgttgg gttccgtttt
     9901 cagcatgttc atcagcgata agatcctgcg cacccgcgcg ttgctgggct gcatgacctt
     9961 tgccaacttc agcattctct ggacctcaat ggcctttttg cttgccgctc caccttttaa
    10021 ctacagcgat ggtgacattg gtctgtttgg gcttgcggga gctgccggag cgttgggcgc
    10081 tcgtccggcg ggcggttttg ccgacaaggg caaatcgcac cacaactttc ggtctgcttc
    10141 tgctgttact ttcatggctg gcaatctggt ttggtcacac ttccgtactg gcgttgatta
    10201 tcggcattct ggtgctggac ctcaccgtac agggcgtgca tatcactaac cagacggtaa
    10261 tttatcgaat acatcctgat gcgcgtaatc gcctgaccgc aggttacatg accagctact
    10321 ttattggcgg tgccgccggt tcgctaattt cagcctcagc ctggcaacat ggcggttggg
    10381 ctggcgtttg tctggctggc gcgacgattg ccctggttaa cttactggtc tggtggcgag
    10441 gttttcatcg tcaggaagcc gcaaattaag caatcagcaa ccgttttcgt ggctttacac
    10501 ttataagggt gttaagaagc ccatcagtct gataaggtta agatattcat tcagtctatt
    10561 tataatatta acaatcgtta agcgtacact ctatggaaag ccctactcca cagcctgctc
    10621 ctggttcggc gaccttcatg gaaggatgca aagacagttt accgattgtt attagttata
    10681 ttccggtggc ctttgcgttc ggtctgaatg cgacccgtct gggattctct cctctcgaaa
    10741 gcgttttttt ctcctgcatc atttatgcag gcgcgagcca gttcgtcatt accgcgatgc
    10801 tggcagccgg gagtagtttg tgggttgctg cactgagcgt catggcaatg gatgttcgcc
    10861 atgttttgta tggcccgtca ctgcgtagcc gtattattca gcgtctgcaa aaatcgaaaa
    10921 ccgccctatg ggcgtttggc ctgacggatg aggtttttgc cgccgccacc gcaaagctgg
    10981 tacgcaataa tcgccgctgg agcgagaact ggatgatcgg cattgccttc agttcatggt
    11041 catcgtgggt atttggtacg gtaatagggg cattctccgg cagcggcttg ctgcaaggtt
    11101 atcccgccgt tgaagctgca ttaggtttta tgcttccggc actctttatg agtttcctgc
    11161 tcgcctcttt ccagcgcaaa caatctcttt gcgttaccgc agctttagtt ggtgcccttg
    11221 caggcgtaac gctattttct attcccgtcg ccattctggc aggcattgtc tgtggctgcc
    11281 tcactgcgtt aatccaggca ttctggcaag gagcgcccga tgagctatga ggttctgctg
    11341 cttgggttac tggttggcgc ggcgaattat tgcttccgct atttgccgct gcgcctgcgt
    11401 gtgggtaatg cccgcccaac caaacgtggc gcggtaggta ttttgctcga caccattggc
    11461 atcgcctcga tatgcgctct gctggttgtc tctaccgcac cagaagtgat gcacgataca
    11521 cgccgtttcg tgcccacgct ggtcggcttc gcggtactgg gtgccagttt ctataaaaca
    11581 cgcagcatta tcatcccaac actgcttagt gcgctggcct atgggctcgc ctggaaagtg
    11641 atggcgatta tataacccac aagaatcatt tttctaaaac aatacattta ctttatttgt
    11701 cactgtcgtt actatatcgg ctgaaattaa tgaggtcata cccaaatgga tagttcgttt
    11761 acgcccattg aacaaatgct aaaatttcgc gccagccgcc acgaagattt tccttatcag
    11821 gagatccttc tgactcgtct ttgcatgcac atgcaaagca agctgctgga gaaccgcaat
    11881 aaaatgctga aggctcaggg gattaacgag acgttgttta tggcgttgat tacgctggag
    11941 tctcaggaaa accacagtat tcagccttct gaattaagtt gtgctcttgg atcatcccgt
    12001 accaacgcga cgcgtattgc cgatgaactg gaaaaacgcg gttggatcga acgtcgtaaa
    12061 agcgataacg atcgccgctg cctgcatctg caattaacgg aaaaaggtca cgagtttttg
    12121 cgcgaggttt taccaccgca gcataactgc ctgcatcaac tctggtccgc gctcagcaca
    12181 acagaaaaag atcagctcga gcaaatcacc cgcaaattgc tctcccgtct cgaccagatg
    12241 gaacaagacg gtgtggttct cgaagcgatg agctaacgcg tcatctcgct caaaaatcca
    12301 gatttataaa agaaaaaatg accggccagc atcgcaacat gctggccttt ttggcaagca
    12361 ggtcggctca gccgatgagt taagaagatc gtggagaaca atatgagcgc aaatgcggag
    12421 actcaaaccc cgcagcaacc ggtaaagaag agcggcaaac gtaagcgtct gctcctcctt
    12481 ctcaccttgc tctttataat tattgccgta gcgataggga tttattggtt tttggtactg
    12541 cgtcatttcg aagaaaccga tgacgcatac gtggcaggga atcagattca aattatgtct
    12601 caggtgtctg gcagcgtgac gaaagtctgg gccgataaca ccgattttgt aaaagaaggc
    12661 gatgttctgg tcactctcga cccgacagat gctcgccagg cgtttgaaaa agccaaaact
    12721 gcactggctt ccagcgttcg ccaaacccac cagctgatga ttaacagcaa acagttgcag
    12781 gcgaatattg aggtgcaaaa aatcgccctc gcgaaagcac aaagcgacta caaccgccgt
    12841 gtgccgctgg gcaatgccaa cctgattggt cgcgaagagc tgcaacacgc ccgcgacgcc
    12901 gtcaccagtg cccaggcgca actggacgtc gctattcaac aatacaatgc caatcaggcg
    12961 atgattctgg ggactaaact ggaagatcag cctgccgtgc aacaggctgc caccgaagta
    13021 cgtaacgcct agctggcgct ggagcgtacc cgtattgtca gtccgatgac cggttatgtt
    13081 tcccgtcgtg ccgtgcaacc gggtgcgcaa attagcccaa cgacgccgct gatggcggtc
    13141 gttccagcca ccaatatgtg ggtggatgcc aactttaaag agacgcagat tgccaatatg
    13201 cgcatcggtc agccggtcac catcaccacc gatatttacg gcgatgatgt gaaatacacc
    13261 ggtaaagtgg ttggtctgga tatgggcaca ggtagcgcgt tctcactgct tccggcgcaa
    13321 aatgcgaccg gtaactggat caaagtcgtt cagcgtctac ctgtgcgtat cgaactggac
    13381 cagaaacagc tggagcaata cccgctgcgc attggtttgt ccacgctggt gagcgtcaat
    13441 accactaacc gtgacggtca ggtactggca aataaagtac gttcaacacc ggtcgcggta
    13501 agtaccgcgc gtgaaatcag cctggcacct gtcaataaac tgatcgacga tatcgtaaaa
    13561 gctaacgctg gctaatccag aggtgcgtgt gatgcaacag caaaaaccgc tggaaggcgc
    13621 gcaactggtc attatgacga ttgcgctgtc actggcgaca ttcatgcagg tgctgaactc
    13681 caccattgct aacgtggcga tccccactat cgccgggaat ctgggctcat cgctcagcca
    13741 aggaacgtgg gtaatcactt ctttcggggt ggcgaatgcc atctcgatcc cgcttaccgg
    13801 ctggctggca aagcgcgtcg gggaagtgaa actgttcctt tggtccacca tcgcctttgc
    13861 tattgcgtct tgggcgtgtg gtgtctccag cagcctgaat atgctgatct tcttccgcgt
    13921 gattcagggg attgtcgccg ggccgttgat cccgctttcg caaagtctat tgctgaataa
    13981 ctacccgcca gccaaacgct cgatcgcgct ggcgttgtgg tcgatgacgg tgattgtcgc
    14041 gccaatttgc ggcccgatcc tcggcggtta tatcagcgat aattaccact ggggctggat
    14101 attcttcatc aacgtgccga ttggcgtggc ggtggtgttg atgacactgc aaaccctgcg
    14161 cggacgtgaa acccgcaccg aacggcggcg gattgatgcc gtggggctgg cactgctggt
    14221 tattggtatc ggcagcctgc agattatgct cgaccgcggt aaagagctgg actgtttttc
    14281 atcacaggaa attatcattc ttaccgtggt ggcggtggtg gctatctgct tcctgattgt
    14341 ctgggagctg accgacgata acccgatagt cgatctgtcg ttgtttaagt cgcgcaactt
    14401 caccatcggc tgcttgtgta tcagcctcgc gtatatgctc tacttcggcg ctattgttct
    14461 gctgccgcag ttgttgcagg aggtctacgg ttacacggcg acctgggcag gtttggcctc
    14521 tgcgccggta gggattattc cggtgattct gtcgccgatt atcggccgct tcgcgcataa
    14581 actggatatg cggcggctgg taaccttcag ctttattatg tatgccgtct gcttctactg
    14641 gcgtgcctat acctttgaac caggtatgga tttttgcgcg tcggcctggc cgcagtttat
    14701 ccaggggttt gcggtggtct gcttctttat gccgctgacc accattacgc tgtctggttt
    14761 gccaccggaa cgactggcgg cggcatcgag cctctctaac tttacgcgaa cgctggcggg
    14821 gtctatcggc acgtcgataa ccacgaccat gtggaccaac cgcgagtcga tgcaccatgc
    14881 gcagttgact gagtcggtaa acccgttcaa cccgaatgcc caggcgatgt acagtcaact
    14941 ggaagggctt gggatgacgc aacagcaggc atcaggctgg attgcccagc agatcaccaa
    15001 tcaggggctg attatttccg ccaatgagat cttctggatg tcagccggga tattcctcgt
    15061 actgctgggg ctggtgtggt ttgctaaacc gccatttggc gcaggtggcg gcggaggcgg
    15121 tgcgcactaa gtacaactaa gccagttcat ttgaactggc ttttttcaat taattgtgaa
    15181 gatagtttac tgactagatg tgcagttcct gcaacttctc tttcggcagt gccagttctt
    15241 cgttgctgtt gatgcgtacc tcacgttcca gaatgctacg cgcaatatcc tgcgcttcct
    15301 gcaacgagtg catctggtaa gtgccacact ggtagacgtt cagttccggg atctgattct
    15361 gatcctgcac tttcagcacg tcttccattg ccgctttcca ggcatcagca acacgctgct
    15421 catctggcgt accaatcaga ctcatataaa aaccggtgcg gcagcccatt ggcgagatat
    15481 cgataatctc tacaccatta ccgttaagat ggttacgcat aaaaccagca aacaggtgct
    15541 ccagggtatg gatccctctt tctggcatca cttctttgtt cggcacgcag aagcgcagat
    15601 cgaacacggt gattgcgtcg ccatgcgggg tgttcattgt tttcgccacc cgaactgcag
    15661 gcgcttccat ccgggtatga tcgactgtga agctatctaa caacggcatt tagccacctc
    15721 cggtaatttt tttaaaaatt ttctgaactc tttcttccca ggcgagtctg agtatatgaa
    15781 agacgcgcat ttgttatcat catccctgaa ttcagagatg aaattttggc cactcccgag
    15841 tggccttttt cttttctgtc aggcgtgttt ttccagccac accgcaaacg gttcggtatc
    15901 agcggtttcc atttcctgct gacggcgttc agatgcctcg cgctcggcta caaaatcctc
    15961 ttcgcgcaga atttccagcg gctcttcacg cagcagatta cggtaggctt ctgcaaacgc
    16021 tttgcctgtt ccgccaatac cagtatcaat catagacctt aagatacggg cagagaaagt
    16081 cagatcggga ttatcgaagc aggcaaccag ttcatcacac actttctgat acgcttcgcc
    16141 gccgttaatg ctatccagcg tttgcgcgac gcgtttcaga tcgcggaaca gatctttacc
    16201 cacctgcagt aacgggaact gcgcggtttc gcagccgata cccagcgtca ggcccggttt
    16261 gcgaccttca aggatcaccc ggttccagtt aacgcgcgta caggcaagtt cgctactgct
    16321 catttccggt gcatcagcca gcgcacacca gaccataaac aggtcgagga atcgcacctg
    16381 ctgttcatct acaccaatcg gcgagaacgg gttgatgtcc agcgaacgca cttcaatata
    16441 ttcaatgccg ccacgtaaca gcgcatcaga aggtgactcg ccgctgcggg taacgcgttt
    16501 tggacgaatc ggcgcataca gttcgttttc aatctgcagc acgttgctat tgatttgcag
    16561 cctcttaccg tctttctcaa taccaatctt cgcgtactct tccgatggcg ttttgattgc
    16621 ctgtttaagg cccgctacgt actcgtaaag atcgttgaag gtaataccaa gattgctttg
    16681 cgatttattg gtatagccga gatcgctcaa acgaagagag gtcgcatacg gcaggtaata
    16741 cataccgcac tcggttttct caaacggcag cgacgttggt tttccttgca ggaaagaaga
    16801 acaaatcgcc ggagatgcac caaacagata aggaataacc caaccgaaac gatagtaatt
    16861 gcggataacg cggaaatagc ccgcagaaat tttctctttg gcatcagcgc ccgagatatc
    16921 accgcacttc gcttgccaga atgccattgg caaagagaaa ttgtagtgca cgccggaaat
    16981 ggtttgcatc agcgcgccgt agcgattttt cagcccttca cgatacagcg ttttaaagcg
    17041 tccggtgtta gaagtgccgt actgtgccag ttcgatgtcc tgaccttctg cgatgtagca
    17101 tggcatactt aacggccaca tccgctcatc gcccatattg cgcgccgtat aacgatgcag
    17161 atcgcgcata aaggtcagca tatgttcaat atcaccatcc actggtgtaa tgaattccag
    17221 caatgcttcc gcaaaatcgg tagtaatcca tttgtgcgtc agtgcggaac ctaatgcttc
    17281 aggatgacct gttgttgcca gtgtgccatc agcattaaca cgcaaagttt cgcgctccag
    17341 cccacgctgt atccccttta acgcctgagg atgtttttcc agccaggcca gcgcctgtga
    17401 tacgtccggg atcaaattga cctcccgcct gtcaaaatcg ttttaattag cataactgta
    17461 atggtgacca tatgtgcagg cctacaatta gtgccaccac atcatgccct gaacggtcgc
    17521 tgctgcaact gcaacatagc gtaacgcttt accaaggcac aaaaaaaaga ttactggccc
    17581 ccaggagatg cgcatccatc ccgctaacag acacagtaaa tcgccaacca cgggcatcca
    17641 gcttaataat agcgtgactg caccatagcg tttcagccag ccggtagctt tctcttgcca
    17701 gcgcgatgtt ttacgcaatg gaaagaaacg cccaaggata acgttagtta accctccaag
    17761 gctattaccc attgttgctg ttaaaactaa aacccaggga tgactgatcc cggaaagcaa
    17821 cattgccacc agcacgactt cggagttgcc gggtaatagt gtagcgctga gaaaactact
    17881 ggcaaacaac gagaaaagcg ataacgcttc actcacagca agcgaacatc cacagcgtcc
    17941 atgcctgctg cacgggccgc ctgaataccg aaatcggcat cttcaaagac cacacactgc
    18001 gtcggttgca cgcccatacg ctgcgcgcac aacaaaaatg tgtctggcgc gggtttatgg
    18061 tgtttgacgt gatcagcagc gacgacggcg tcaaaataac ggcgtaatcc caggtgcgcc
    18121 agcaatgcct cagcgatggc gctttcactc cccgttccta cagccattgg gcgacgacca
    18181 tgccaacttt tcaccacttc aacaagagga agcggctcga cactatccag cagcatactt
    18241 cttactgctt ctgttttttc acgcgctaac gcatgcgggt cgagatcggc ctgattcagc
    18301 tcaataattg cctgagcaat acgccaggtg ggcgatccat taagcgcaat catcgcctga
    18361 atatcgtact gaagaccgta gtgccctaat acttcgcgcc acgctttacg gtgcgtaggc
    18421 gccgtatcca ggattgtgcc atccatatca aaaattaaac ctgcataacg ctcgtacatg
    18481 gtcttctcgc aagtcgaaaa atatgacgct actttagcgt aattgcttga ttttgtcgct
    18541 gatggcgaag gagaagaaaa ttcaggaaat ggataaagta gttatttaga aatgagatat
    18601 ttttgaagga aattttttgg agaatatggt gcatccggga ggattcgaac ctccgaccgc
    18661 tcggttcgta gccgagtact ctatccagct gagctacgga t
//
LOCUS       DAZSNO010000069        18596 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZSNO010000069 DAZSNO010000000
VERSION     DAZSNO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18596
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.069"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1740
                     /gene="adeD"
                     /locus_tag="ACHWN2_002286"
     CDS             <1..1740
                     /gene="adeD"
                     /locus_tag="ACHWN2_002286"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR8931802.1"
                     /translation="HHISRAEYQELLAVSRGDAVADYIIDNVSILDLINAGEISGPIV
                     IKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRG
                     LTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLE
                     QMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGLGGKELNAYI
                     AAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNSPQCMLCTDD
                     RNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADI
                     VLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQPVSASDFALQ
                     FTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGG
                     FGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLP
                     IAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFD
                     GEKFAFTTLEVTE"
     gene            complement(1786..3177)
                     /gene="uhpT"
                     /locus_tag="ACHWN2_002287"
     CDS             complement(1786..3177)
                     /gene="uhpT"
                     /locus_tag="ACHWN2_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR8931803.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3315..4634)
                     /gene="uhpC"
                     /locus_tag="ACHWN2_002288"
     CDS             complement(3315..4634)
                     /gene="uhpC"
                     /locus_tag="ACHWN2_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR8931804.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4644..6146)
                     /gene="uhpB"
                     /locus_tag="ACHWN2_002289"
     CDS             complement(4644..6146)
                     /gene="uhpB"
                     /locus_tag="ACHWN2_002289"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR8931805.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6146..6736)
                     /gene="uhpA"
                     /locus_tag="ACHWN2_002290"
     CDS             complement(6146..6736)
                     /gene="uhpA"
                     /locus_tag="ACHWN2_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR8931806.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6811..7101)
                     /gene="ilvN"
                     /locus_tag="ACHWN2_002291"
     CDS             complement(6811..7101)
                     /gene="ilvN"
                     /locus_tag="ACHWN2_002291"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR8931807.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7105..8793)
                     /gene="ilvB"
                     /locus_tag="ACHWN2_002292"
     CDS             complement(7105..8793)
                     /gene="ilvB"
                     /locus_tag="ACHWN2_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR8931808.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8899..8997)
                     /gene="ivbL"
                     /locus_tag="ACHWN2_002293"
     CDS             complement(8899..8997)
                     /gene="ivbL"
                     /locus_tag="ACHWN2_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR8931809.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9562..9651
                     /gene="tisB"
                     /locus_tag="ACHWN2_002294"
     CDS             9562..9651
                     /gene="tisB"
                     /locus_tag="ACHWN2_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR8931810.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9775..9849)
                     /gene="ysdE"
                     /locus_tag="ACHWN2_002295"
     CDS             complement(9775..9849)
                     /gene="ysdE"
                     /locus_tag="ACHWN2_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR8931811.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9931..11115
                     /gene="emrD"
                     /locus_tag="ACHWN2_002296"
     CDS             9931..11115
                     /gene="emrD"
                     /locus_tag="ACHWN2_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR8931812.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11123..11620)
                     /locus_tag="ACHWN2_002297"
     CDS             complement(11123..11620)
                     /locus_tag="ACHWN2_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR8931813.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11617..11979)
                     /locus_tag="ACHWN2_002298"
     CDS             complement(11617..11979)
                     /locus_tag="ACHWN2_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR8931814.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11969..12316)
                     /locus_tag="ACHWN2_002299"
     CDS             complement(11969..12316)
                     /locus_tag="ACHWN2_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR8931815.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12425..12838
                     /gene="yidI"
                     /locus_tag="ACHWN2_002300"
     CDS             12425..12838
                     /gene="yidI"
                     /locus_tag="ACHWN2_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR8931816.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12885..14327)
                     /locus_tag="ACHWN2_002301"
                     /pseudo
     CDS             complement(12885..14327)
                     /locus_tag="ACHWN2_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(14362..16077)
                     /locus_tag="ACHWN2_002302"
     CDS             complement(14362..16077)
                     /locus_tag="ACHWN2_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFR8931817.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            16244..17137
                     /locus_tag="ACHWN2_002303"
     CDS             16244..17137
                     /locus_tag="ACHWN2_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR8931818.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17134..17948)
                     /locus_tag="ACHWN2_002304"
                     /pseudo
     CDS             complement(17134..17948)
                     /locus_tag="ACHWN2_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17948..>18596)
                     /locus_tag="ACHWN2_002305"
     CDS             complement(17948..>18596)
                     /locus_tag="ACHWN2_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR8931819.1"
                     /translation="TEPLEFTFLFISPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLI
                     DQVLPQNWIPMFSNHADMMLTQIAIGLCFTLLYFVVFRTLILQFNMCTPGREDAEVKL
                     YSKAEYKASRGQTTAAEPKKELDQAAGILQALGGVGNISSINNCATRLRIALHDMSQT
                     LDDEVFKKLGAHGVFRSGDAIQVIIGLHVSQLREQLDSLINSHQSAENVAITEAV"
BASE COUNT         4503 a         5078 c         4792 g         4223 t
ORIGIN      
        1 catcacatta gccgggctga ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt
       61 gccgattata ttattgataa tgtctctatt ctcgacctga tcaatgccgg agaaatttcc
      121 ggcccaattg tgattaaagg acgttacatt gccggtgttg gcgcagaata cgctgatact
      181 ccggctttgc agcggattga tgctcgcggc gcaacggcgg tgccagggtt tattgatgct
      241 cacctgcata ttgaatccag catgatgacg ccggtcactt ttgaaaccgc taccctgccg
      301 cgcggcctga cgaccgttat ttgcgaccct catgaaatcg tcaacgtgat gggagaagcc
      361 ggattcgcct ggtttgcccg ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc
      421 agctcttgcg tgcccgctct ggaaggctgc gatgttaacg gtgccagttt tacccttgaa
      481 cagatgctcg cctggcggga ccatccgcag gttaccggcc ttgcagaaat gatggactac
      541 cctggcgtaa ttaacgggca gaatgcgctg ctcgataaac tcgatgcatt tcgctacctg
      601 acgctggacg gtcactgtcc gggtttgggt ggcaaagaac ttaacgccta tattgctgcg
      661 ggtattgaaa actgccacga aagttatcag ctggaagaag gacgccggaa attacaactc
      721 ggcatgtcgt tgatgatccg cgaagggtcc gctgccagca atctcaacgc actggcaccg
      781 ttgatcaacg aatttaacag cccgcaatgc atgctctgta ccgatgaccg taacccgtgg
      841 gagatcgccc atgaagggca tatcgatgcc ttaattcgcc gcctgatcga acaacacaat
      901 gtgccgctgc atgtggcata tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg
      961 aatcacctcg gcttactggc acccggcaag caggccgata tcgtcctgtt gagcgatgcg
     1021 cgtaaggtca cggtgcagca ggtactggtg aaaggcgaac cgattgatgc gcaaacctta
     1081 caggcggaag agtcggcgag actggcgctg tctgccccgc catatggcaa taccattgcc
     1141 cgccagccag tttccgccag cgactttgcc ctgcaattca cgcccggaaa acgctatcgg
     1201 gtcattgacg tcatccataa cgaattgatt acgcactccc gctccagcgt ctacagcgaa
     1261 aatggttttg atcgcgatga tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg
     1321 ctggctccgg cctgtggttt gctcggcggc tttggcctga atgaaggcgc gctggctgcg
     1381 acagtcagcc atgacagcca taatattgtg gtgatcggtc gcagtgccga agagatggcg
     1441 ctggcggtca atcaggtgat tcaggatggc ggcgggctgt gcgtggtacg taacggccaa
     1501 gtacaaagtc atctgccgtt acccattgcc gggctgatga gcaccgacac ggcgcagtcg
     1561 ctggcggaac aaattgacgc cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat
     1621 gagccgttta ttcagatggc gtttctttct ctgccagtga tccccgcgct aaaactaacc
     1681 agtcaggggc tatttgatgg cgagaagttt gccttcacta cgctggaagt cacggaataa
     1741 taaaaaaagc ccggcgtcat gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa
     1801 ctgctgaatt tttttctcgc ggcggatttt gcgttcttcc attaccgcca ctatcgccat
     1861 caggcagata caaccaatcg cggcgatatc cagcgcggcg aaggtgcctg cccagccggt
     1921 aaggccgaat accggcgtcc catcggcaat cattcccaga cctaacttgg caaagctgtc
     1981 accaatcagg taagcaaagg tgcctttaat accatcggca gcgccaatcg cttttttagg
     2041 tacaaagcca acagcagcca caccaatcaa caattgcggg ccaaagacca ggaaacccaa
     2101 cgcaaagaga gaagccagat agatatattc gttactggca tgttgataca caccgagcgt
     2161 ggcgataatc agcgccagcg cgatgcaggc caccaggcca cggcgaccgt tcgccaggtc
     2221 agagagccag ccccacagca gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc
     2281 ctgaatcgcc accgctttag agagtttcag ttcctggaac gcgtatacgg ttgaccactg
     2341 gtcgatacca atacgtacca catagaggaa aatgttggcg aagcacagca gccagatcac
     2401 tttgtttttc agcacatact caacaaagat ctgccacttg gtcatatcgg tagattctgt
     2461 ctctttgtcc tcttcgctga tctcctcgcc gaacagttct tcagctttgc cgaggccata
     2521 agattccggg gagtcgctgc cgtaacgcag gccgataaaa ccgacaatca gcgcgataat
     2581 cgacgggaag ataaacatgc cgatgacatg gccatcgaac aggtaatttg ccccgaacag
     2641 cgccacacct gctgcgcctg caccgccaag gttgtgagaa atattccaga aaccgaggaa
     2701 tgtcccgcgt ttacgacgcg gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc
     2761 ggtactctgg aaaaagccgc ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga
     2821 gccgctgccc atactggcac tgaagcccag catacaaatg gcagagagga taagcatgaa
     2881 cggcaggaat tgtttggtgt ttttgccgtc ggcgtagtat gaaaccaacg ttttacccac
     2941 gccataagtg atggagaaac ccaggccgat catccccagc tgcgtcatgc tcaacccgta
     3001 ggtcgaaatc atatcgttct gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag
     3061 gtagccgata aagaccacca ggtaggattg catgaacggt ttgaaccaca ttttgcgccg
     3121 cacttcgagc ggaaggtcca gggtcggctt gcgaacttgg tttaggaaag ccagcatggg
     3181 ttactcctga aatgaatacc tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg
     3241 cctgagatag cgtccaagaa aaacctggaa aatttcttag ttttgccgga tatgaagtga
     3301 aaaggtgaga tgcatcacgc ttcacgcggt gtctgggcgt tcaaaaaggg cagtaacagc
     3361 agtgcggaaa tcccggcggc gatagagata accacaaaaa atccgctcca gtgccaggta
     3421 tcgagcactt tcgccagcgg ccaaccagca agcgacgccc ccagataagc aaacaagccg
     3481 acaaaccccg tcgccgcccc tgccgcctct ttgtgggaac actctgccgc cgccatacca
     3541 attaacatct gcgggccaaa gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc
     3601 acgtagctgg caaatggcat cagccacagc gagccgactg aaagcaaaat tccggcggcg
     3661 aaaatcaaat tcatcggccc acggttgccg ttaaacaatt tgtccgagcc ccaaccggct
     3721 accagcgcac cgataaatcc gcccagttca aacatcgtca ctgccgtatt cgccgtgacc
     3781 agatcgacgc ccagcgtctc ggacatatac aaattgcccc agtcgttgat cgccgcccgg
     3841 accacataga ccagcacata gcaaaacgaa agcagccaga tatacggatt cagcaacaca
     3901 tatttggtga ggatctcttt acgcgtcaac cctgcccctt cttgttgttg agcaatttcc
     3961 agcgcgtcgt gccgccattc accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgt
     4021 agccgccagc agagaaaaat ccccacgact atcgccatac aaccagcaat catcatcccg
     4081 gcacgccagc cgtaatgcag cgcgctcgct gccatcacaa tgggaatgag tgcgccgccg
     4141 acgttatgcg ccgtgttcca taatgcccac caaccgccgc gctcggtacg tgaataccag
     4201 gccgttaaca gacgcgcaca caccggtgaa ccccagccct ggaaaaaggc gttcagcacc
     4261 cagagcacgg caaacgccca tagcgacgtc gagaagccaa acagaatgtt catgatgccc
     4321 gtggcgataa gccctatccc cataaaataa cgggcatttg agcgatcgct gacaatgccg
     4381 gagacaaact tcgacacgcc gtaagtaatg taaaacaggg tcgctaacag gccgatatcg
     4441 ctacggctga gcacgccgtt agcaaggatt tctggtaccg cggcgttaaa acttttccgc
     4501 gtgaagtaaa acagcgcgta acccagccag atggtcagca gaatatgccg acgccagtag
     4561 cgataacggg catcaatttc atgtttatca gtcattaatg gcgcatcggc aggcgctttc
     4621 agaaacggca acatcatcga accttagaca taacgttgag gtagagaaac gctgacacgc
     4681 gtgccgtgca gacaggaaat gtgtaatgtg ccgcccagcg ccgttacgcg ctcgcgcatt
     4741 ccggtgaggc caaaaccttg ttgcccggaa cccggcggca aaccgctgcc atcgtcttca
     4801 atcaccagca tcaaccgttc atcctgctgc cagccttgca gcgtaaccgc gctggcatcg
     4861 gcatgtttca caatgttgtt cagcccttcc tggcagacac gaaacagcgt cacgcgctgg
     4921 ttttcgctta atgctgattc atcgatacgc cattcgagat ggctgacaat gccgcgccct
     4981 tccagctcca tttcccgcat cagtgagcgg atggcctgct ccagggtgag atcatccagc
     5041 tggcgcggac gtaaccgtcc taacaaacgg cgcaccgcgt cgtaaacgcc cagcgacagt
     5101 tgttcgatga gctgcccgct ctgcttcaca ctggcgttat ctgccgccag ccgctgaaca
     5161 atgcccgcct gagtacgaat agcagtgatg gtctgaccga tatcgtcgtg cagctcacgc
     5221 gccacgtcgc ggcgcacgct ctcttcggtt tctaacaaac gttcagccag atgctgattg
     5281 cgcgccagtt ccttttgtag cgactggtta agttcacgca accgctggat gcctgcgccc
     5341 agtaacaacc ctgtcagact ttgcaccagc aacgagagta ataaatccac cagatgatcg
     5401 cgccaggttt gactggcgat cagcgcgatg gcgttcatca acgtcgcaat cagcgctcct
     5461 tgccagccat agtgccaggc cagcgcgata atcggcagcg ccagacagaa tggcgtaaag
     5521 cgcgacagtt cgtccggcaa tcccaactgg agccagagac tgataacaaa cagcagcaag
     5581 taccagacca gatgccgccc gcgccagttg atgggctgag aaaccagtga cgggccgagc
     5641 ggcagccagg tgttattggc gagatagtgc cagaacacca gacatatcgg ggccagcgtc
     5701 agaccgccag ttaaagtcag caacagcgca ttccacgact ctttgccgtg ccaaagccag
     5761 ggcagcgact gcaacaacgc cgccgccgtt aacgccgcac cctgtaacag caaggtgcgc
     5821 cagtcacgct gatggcgata gcgcgagatc agcgctacgg gcagtaacgt cagcaaacta
     5881 ccgatcatca ataacggaaa atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc
     5941 agccactccg cacccagcaa tacgggccag tatccgcgcg ggcattgcag cattagcccc
     6001 agacgcagac caaacggaaa taacagcacc gccatatcag ggcgctcaac cagatgcagg
     6061 ctgatactcc ataggcaaaa ccatgcggca gagaagataa aaaagcaggc aataacggta
     6121 attaagcggg agaacaacgt cttcatcacc agccatcaaa catgcggcgc gccagctcta
     6181 cgtcgttact gacgcccagt ttttccatca gattggcgcg atggacgtgt accgttttcg
     6241 gtgacaagcc cagttcggcg gcaatctctt tcaccgccat tccttgcgcc agtttttccg
     6301 ccacctggcg ttcacgtttg gtcagcgggt cctgacgacc ggatgccagt ttaatggcaa
     6361 tatccggcgt cagataacag ccgcccgtgg caaccgtatg caccgcagca atgagttcat
     6421 ccgggctaca gcgtttggaa agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca
     6481 gcgcaggact gtcgtgaacg gagagcataa tcgtcgccat acctttcggc agctggctta
     6541 gcagctccag accggagata tcgggcatgg agatatcgca aatacacacc tgcacaccgc
     6601 gccccggcag ccccgccagc gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat
     6661 caggttccag ccccagcagc tgcgcaaagc cggagcggac gatgaggtga tcgtctataa
     6721 gggcaacggt gatcatggtc ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga
     6781 taagcgcgat gttgttcaag ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa
     6841 catcgtcgga tcggactgat tacgctgcac tttcacgaca tcttccagct tagcgatttg
     6901 gcttatcatc tgctccagac gctggtcgtc attgaccagt agccagatat ggcttttgtc
     6961 gctgtcctga atcggcagac aaagaatgcc ttcaacgtta aaagcgcggc gggcaaaaag
     7021 gccacaaacg tgggtcatta cgcccggatg gttgcgaacg gtgagctcca gaattacgtt
     7081 gttatgagtt gtgttttgca tggcttattc ccccaccatt tcagtattcg ccgcacctgg
     7141 cggcaccatc gggtaaactt tttcttcggc atcaatgcgc acatggatca gcgccgggcc
     7201 agggcgattg atgatttcct gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca
     7261 ggtttcgagg ccgaatccgg cggcaatctg cataaagttg attttgcccg gataggtggc
     7321 ggcaaaaacg ccttgctcgt agaacagact ctgttgctga tgcaccagcc ccagcgcttc
     7381 gttgttcatc agaatgattt tgacatccag ctgattttca ctggcggtcg ccatctcctg
     7441 aatattcatc atcaggctgc cgtcgccgga gaaacacaac actttgcgat ccgggttcgc
     7501 cagcgccgcg ccaatcgccg caggcaggcc aaaacccatc gtgcccagcc caccggaggt
     7561 cagccactgg cgtgggcgat tgagcggata agcttgcgcg gtccacatct gatgctgacc
     7621 cacatcggtg gtgataatcg cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc
     7681 gtaatggctt aatggatcgc acgctttcgg gattggacac gggaattcgc gctgcaaatc
     7741 cgctaccaac tggtgccact ctgcacgcgg ttgcgcttcc acccgcggga tcaattgcgc
     7801 cagcacgtca tcaacatccg cctgaatcgc cacatgcggc tgcttgattt tacccagctc
     7861 tgcgcgatcg atatcgacat gaataatttt ggcattcgga cagaactgct cggttttgcc
     7921 aatcgcccgg tcatcaaaac gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat
     7981 atagttggta ctgcgcacgc cgtgcatccc cagcataccc agcgacaacg gatgcgcttt
     8041 tggcagcatg cccagcgcca ttaaagtcat ggtggtaggc agttgcgctt tctccgccag
     8101 ttcacgcacc cgcgcaggcg cattgatcac accaccaccc agataaagca ccgggcgttt
     8161 ggcagcgtta atcattgtag ctgcgtcacg aatgctttct tcgctaaagg cgggtgcaac
     8221 ggctttttct gccaccgcgg gctgagcttc aatctcaaaa accgccgttt gtacatcctt
     8281 aggaatgtct atccacaccg ggctagggcg gcctgattgc gcaatgcgga aggcatcgct
     8341 catgacctgt gggagctctt cgatatgtct gaccagatag ttgtgtttgg tgatggggat
     8401 agagatgccg taggtgtcca cttcttggaa ggcgtcggtg ccgatcatcg aggcgggaac
     8461 ctgaccagtg atgcaaatca gcgggatgga gtccagccgc gcatcagcaa tggcggtcac
     8521 caggttagtc gcacccggcc cgctacaggc catacagacc gccggtttac cgtcggtgcg
     8581 cgccattccc tgagcgataa accccgcgcc ctgttcatgg cgagccagaa tatggcggat
     8641 ttgcgtgctt tggcttaagg cgtcgtaaac aggcaggata gaaccgcccg gaatgcccgt
     8701 cacaatctta atgccctgct gttccaggaa atgaacgata aattctgcgc cggtaaagcg
     8761 cttacgcgtc gatgttgtgc ccgaacttgc catgctccag tccttttctt ctgggccgac
     8821 tttccgggag gtgcttaaac gaaaaacccc gcccggtttg cgccggcggg gttttggaat
     8881 cgtgtgttgt tccagtccct acggcgcatt gccgacgacc accaccacac gcacgacgac
     8941 cactgcggcg gatggcgcag ttggtagtag ttttgcgttg aacatggaag tagtcattgg
     9001 ggaccttgtt ggttttgtgt ttaacaatat ttatacaagc acagctttac aggggagaca
     9061 atggaaaatt tttcagcaag ggaaaattga ggggttgatc acgttttgta ctgaattgca
     9121 gataacaaaa aaccccgccg gagcgaggtt tcgtcagccg cctgcggctg gtaaccgcaa
     9181 agcacactgt attatgtcaa cactgaaagt atacgtgttc cgcgcagaac gcgcaatttc
     9241 ggcacgaatt ttgacgtatt tagtgcatag ttgagtatcg ttcacagttt gcgttttgtc
     9301 caaatattac tgtttattta tacagtaagc ttctataata tcactgtacg caatgtgtta
     9361 tgcgggggcc gcatcgttac ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca
     9421 gcgccttaac cccgcgtgag cacactgtgt tatgtcaaca agcacaacgt ttctccttga
     9481 gataccgcgt gcacaacagc tggcaacagg cagcggaaag gtacgtcagc tggcagtgct
     9541 cctgaaccac aggagacgcg tatgagcctg gtggatatcg ccattcttat cctcaaactc
     9601 attgttgcag cactgcaact gcttgatgct gttctgaaat acctgaagta attcagattc
     9661 aagtcgcacc aaaggggagc gggtaaccgc tcccctttta tatttagcgt gcgggttgga
     9721 gtcggatgcg atgctgacgc atcttatccg ccctaccatc cctcccggca acatttattg
     9781 ccgcttttgt ttacatattc tgccgctaaa caattcccca ttcctggcgt atatctggct
     9841 aacattcatc aatgtgatag attcctctcc cgcatttatg ggaatgcgta gtgacttatt
     9901 ctaattattt ttataaaagc atccgtgata atgaaaaggc aaagaaacgt caatttgtta
     9961 ttgatgttgg tattactcgt ggccgtcggt cagatggcgc aaaccattta tattccagct
    10021 attgccgata tggcgcgcga tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc
    10081 gcttatctgc tgacttacgg tgtctcacag ctgttttatg gcccgatatc cgaccgtgtc
    10141 ggtcgccgac cagtgatcct cgtcggaatg tccattttta tgctggcaac gctggtcgcg
    10201 gtcacgacct ccagtttgac ggtattgatt gccgccagcg cgatgcaggg gatgggcacc
    10261 ggcgttggcg gcgtaatggc gcgtactttg ccgcgtgatt tatatgaacg gacacagttg
    10321 cgccatgcta acagcctgtt aaacatgggg attctcgtca gtccgttgct cgcaccgcta
    10381 atcggcggtc tgctggatac gatgtggaac tggcgcgcct gttatctctt tttgttggtt
    10441 ctttgtgctg gtgtgacctt cagtatggcc cgctggatgc cggaaacgcg tccggtcgat
    10501 gcaccgcgca cgcgcctgct taccagttat aaaacgcttt tcggtaacag cggttttaac
    10561 tgttatttgc tgatgctgat tggcggtctg gccgggattg ctgcctttga agcctgctcc
    10621 ggcgtgctga tgggcgcggt gttagggctg agcagtatga cggtcagtat tttgtttatt
    10681 ctgccgattc cggcggcatt ttttggggca tggtttgccg ggcgtcctaa taaacgcttc
    10741 tcaacgttga tgtggcagtc ggttatctgc tgcctgctgg ctggcttgct gatgtggatc
    10801 cccgactggt ttggcgtgat gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt
    10861 ttcggtgccg ggatgctgtt tccgctggcg accagcggcg cgatggagcc gttccccttc
    10921 ctggcgggca cggctggtgc gctggtcggc ggtctgcaaa acattggttc cggcgtgctg
    10981 gcgtcgctct ctgcgatgtt gccgcaaacc ggtcagggca gcctggggtt gttgatgacc
    11041 ttaatgggat tgttgatcgt gctgtgctgg ctgccgctgg cgacgcggat gtcgcatcag
    11101 gggcagcccg tttaagcgca cgtcaccgca gcatcgtcat cagctccatg ggagaacgat
    11161 gctgctttat cagatcacgc atcacccgca tatgcggtgc ggagtaagaa taaaacgcct
    11221 gatagcccgc acaaagcacg ctgtctggcg tctcctgccg agcgtgcggg cacaggcgat
    11281 gccagtcgca cgcctggcag tcacggcgcg gttgcgggtt ttcgctgcca caccattttt
    11341 gtaaggtggc gtcaaaaagc tcaatcgaga tacgactgat atctgcccgc acccaacact
    11401 caaacagggc aattaaaaat ttcccccaca gctctgctgt caccgaagcg gccgtcagat
    11461 cgccccggtc atcaaattca agatagggaa tgagctgtac ggagcggatg ccccgctgtt
    11521 tgaaattgtg ataaaaatcg gcaggttgca gagcatcctt gtactctaca accagcttat
    11581 gcctgtcctc ttcagcatca atgacctgac ttcctgtcat gcgactctct caataaatac
    11641 gataagttga tgtatatgcg ttacagcaaa cagtattgcg agagataaca ccgcgaggga
    11701 gatcaaaaat ttgtcacgga ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat
    11761 taaattgcga ttacgggtgt agtgccagag gatcagcgcc acgatggcga ggatgccaat
    11821 cgaaatccag aataacatgc ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa
    11881 cgcgccgtag cccagcatgg tacgaaacca cgccagtgat gtgcgctccg gctgcagccc
    11941 tgggtcggca atgcgtctgg ctttacggct atccggcata caacaccagt cccataacga
    12001 tcaccgccac aaccatcaat attaagctga tgattaataa gctgttggtg tagggcaggt
    12061 cctctttcag acgcatcgct ttttcattgc gtaaccagcg caggtagcca tacattgcta
    12121 acccgccgga aaacagacac aacaacaacg ccagcagttc acgaatgacg ggcgtggcga
    12181 aatctggcgc aagttgatca agcccaaccc ccgccgccag aaatcctagt gcggtacgga
    12241 tccacgccag aaaagtacgc tcattagcca gcgagaagcg gtaatcgggc gcttctccaa
    12301 ggcgggaaat tttcattgtt gctcctttgc aatttcacta acgctctatc tttagcggtt
    12361 ttttttaaga atttcgtccg cccgtttcct gatatgcttt tgccatccgc tacaaggagg
    12421 tcagatgggt atcatagcgc aaaataaaat cagttcattg ggcatgctgt tcggtgccat
    12481 cgctttaatg atggggatta tccacttttc attcggccct ttttccgcac caccgccaac
    12541 gcttgaaagc atcgtggcgg acaaaacggc tgaaattaaa agaggtcttc tcgccggaat
    12601 aaaaggcgaa aaaattacca ctgtggagaa aaaagaggac atggatatcg ataaaatcct
    12661 cgatcaaagc ggtatcgcac tggccattgc tgcgctactt tgcgcattta ttggcggtat
    12721 gcgtaaggaa aatcgctggg gcattcgcgg cgcgctggtt tttggtatcg gcatcgtgtg
    12781 cagcattttg ctgatattcc tgattttttc cttcctgacc ggcggttcgc tggtttaagc
    12841 agcctgaata atgcccgccg ggtgatcatc agcgggcatt atcgtcagaa tttctgtttt
    12901 ttctcctcca ccttcactcc ttgcgtcggt aggccggtgt catagtcgcg taccactggc
    12961 gaatagccat cttgtgggcg tgggcgaaac gcccccatcc agcgcggtcg tgcatcttta
    13021 cgccacggac gcagactcca ttggtaactg cggaacggat cgcgaatttt gtccatgtaa
    13081 tccaataagg cgtcatgcat tttgctgcga acgtctgcaa aacggatatc atcgatcagg
    13141 ttatgcattt catttgggtc attacggcga tcgtaaagtt catcactggt gaagaggttg
    13201 agcaccagtt taaagtcatc cgtcacccag caacgcaccg gaataaaacc gccaaagctg
    13261 tcatgctcaa tctcgtagcg gttaaattcc accatcacgc cgcgtggctc tttcacggca
    13321 aggatatttt cccccggcag aatctctggt ttttcaatat ctgccagcgc catcattgtc
    13381 ggcagtaaat caatatgact gactggcgta tcgacctgtc gccgctcccc ttgcggcgaa
    13441 cggatgatca gcggaatacg ggtgatgtcg tcatacatcg ccgctccttt actgatcagc
    13501 ttatgtgcgc ccatcatttc accgtgatcg gaggtataaa tgacccacgt attttcacgt
    13561 tgctctggcg ttaaggcatt gatgacacgt ccgatttggt catcaacaaa gtcattgcag
    13621 gcaaaataga gcggatggtg ataaagcccg tcatcaccga ctggcgatgg catcgcctgc
    13681 gcccataagc ggtgatgttc cggtttgttc gccaggtcat cctgtgattt ctcgccaagt
    13741 tcgtagtaaa aatcagcgta tttctctaaa tactccaccg gacaggtgaa cgggtgatgc
    13801 ggctcatcat acgaaaccac catcaggaag ggttcctcgg cgcgcacggg ctattgcaga
    13861 aaatctaccg ctcgattgct gatgcggtgc gcccaggtga aggtttcgtc aatatggttc
    13921 gcctgtaaat cttcgacgct gtttaggcca ttgcgccaca ggctgatctc tttttccgtc
    13981 agttcgctaa gatagttcgc cccatcaaac cagtaatcag catcccactc cggcggacac
    14041 tcgccagtgc cgaaatagtc atgaccgtcg agatgccatt tgccgatgta acaggtgtga
    14101 tagccggcat ccttaaagta gcgccccata gtggagatgt ttttgcctgg cgcgacgttg
    14161 ttggtccacg ggccggactg gttagcgtag ataccggtga atagtccggc ccgtgcaggc
    14221 gtacaaaccg gtgaacaagt gtaggcggaa ttaaagcgaa taccttccgc cgccagacta
    14281 tcaatatttt gcgtattcag cggtttaccg ctatagcaac cgaccatatt ggtggcctgg
    14341 gtatcggtca attggggcgt ttcataggct atccttcgcg tcgggagtaa aggttacggc
    14401 tgggtcgtga cgatgccacc agctgtcaaa aatcaggtag acaatcacta cggcagcgat
    14461 gaaataactg atcatcgcta accagcgcgt accgtagccg ccgaattcag ccagcccgac
    14521 atagacgcca atcatcgcga acaggatacc aattgacgcg atcttgacgt ttttccacgg
    14581 tttcatgtcc accgcaaacg catctttgaa ggtgaacggc gtggcgcgcg gtttgataaa
    14641 accgatcacc agcatcacga ccacgttgat gcagaacgta caggccagca catagaggaa
    14701 atggaagtcg aacttcacca gatagttgat ggtgatgtag ctgattatgc caatccccat
    14761 cgccactttt gccgccagcg ccgggatgcg cggaaagaaa aagcccataa tgatgatggt
    14821 aaccagcggc acgttgtaaa tgccgttgag ctgtttcatc cagctataca gcccctgcgg
    14881 cgcgttcgcg atccacggcg ctaccaaaac cgaaacgata gcgataaaga aaccaaattt
    14941 gcgcccgacg gtgaccagct gctgcggctc ggcattctgg ttaatgatgc gacggtaaat
    15001 acccatactg aataatgtac tggcgctatt cagaaagccg ttgaaggtac tgatcaccgc
    15061 accaaataac accgcgccga agaaaccctc cagtggcact ggcagaacgt tattgaccag
    15121 cgtcgggtag gccatgtcgg ctttcggtag atcctgatac agatgaaacg caatcaaccc
    15181 tggcagcacc agtaccagcg ggtcgagcat tttcagcacc gccgttaaca gcgccccctt
    15241 ttgcccttcc gccaggcttt ttgacgccag cgtgcgttgc acgatgccct gatttgtaca
    15301 ccagtaaaag gtgttcacca gaattaaacc ggtaaatgcc gcgccaatcg gcaagggatc
    15361 ggtcgggcca ccgactgagt ttaatttctc ggcgtgaacg gtggtaattt gctcaatgcc
    15421 ctgcataaag ctgcccttgc ctatcgcgat caggccaaat accggcacca tcaacccgcc
    15481 gataaccagc ccaataccgt tgatggagtc cgccactgcc attgcgcgca gtccgccgat
    15541 caccgcatac aaaattcccg ccagaccaag caaaattacc agtagccaga tagccgcacc
    15601 gtgagaaatc tgtagcgatt ccccgacgtg aaacaggctg ttgagcgcca acgcgccgga
    15661 gtagagaaca atcggcagaa agcagacgcc ggtggcaatg aggaagcaga agtcgatgat
    15721 aatacgcgtc gttttatcat aacgttcctc cagaaaatcg gggatggtgg caatgccgcg
    15781 cttcagatag cgcggtaaaa agattagcgc gaggaagatc agcgtcaccg ctgaagtcac
    15841 ttcccagccc atcaccgaca tgccgctttt gtaggcctgc ccggaaagac caaccagttg
    15901 ttccgtggaa aggttggtta acattaacga agcggcaata accggcgctt ttagtgaacg
    15961 tccggcaaga aaatagcctt gttgcgatcc ggtatctgtt ttgcgaacct tccaccaggt
    16021 gatcaccgcc accagcagcg taaaaccgac aaaactcaag atttgtaacg aattcatctc
    16081 atagccctta tttatgttta tgtcccggac gcatgaatat agggagaggt aaaatgcgtc
    16141 gggtcaggca gtcagattct taaaatacaa acgtcgtatc cctgaacgga ttgttttctg
    16201 ttaagttcag gttgtgagca taattcgccg gagggatatt aaaatgaatg gaaaattgca
    16261 aagttcagat gtaaaaaacg aaactccgta caatattccc ttattgatta acgaaaatgt
    16321 gatctccagc ggaatttctc tgatctcgct ctggcatacc tacgccgacg agcattaccg
    16381 ggtgatctgg ccgcgggaca agaagaaacc gcttatcgcc aactcatggg ttgcggtgta
    16441 taccgtacaa ggatgtggga aaattctttt aaagaatggc gaacaaataa ctctgcatag
    16501 caactgtatt atatttttaa agccaatgga tattcactct tatcactgtg aaggtttagt
    16561 ctgggaacag tactggatgg aatttacccc caccagtatg atggatattt ccgttggtca
    16621 gcaaagcgtt atttataatg gcgaaattta taatcaggaa ctcaccgaag ttgctgagtt
    16681 aataacttca ccagaagcaa taaaaaataa tctggcagtc gcttttctga cgaaaattat
    16741 ttatcagtgg atttgtctta tgtccgcaga tggtaaaaaa gatccacaac ggcggcaaat
    16801 tgaaaaatta attgccactt tacatgccag tctgcaacaa cgctggagcg tagctgatat
    16861 ggctgccacg atcccctgta gcgaagcctg gttgcgtcgt ctgtttttac gctataccgg
    16921 caagacgccg aaagaatatt acctcgatgc gcgtctggat ctggcgctat cgctattaaa
    16981 gcaacaagga aactcggttg gcgaagtcgc tgatacgctc aacttcttcg actcctttca
    17041 tttcagcaaa gcctttaaac ataaatttgg ttatgcgccg tcagccgtgc taaagaatac
    17101 ggaccagcac ccaacggatg ccagtccaca caattaacgt aactctggcc agtacgcttt
    17161 gttggcctcc agcaactcat ccagaatagc tttggcgacc gaggcgctcg gtaccgtttt
    17221 cgacaacgta atcgcctgcc acaggtgctg atatgaacgc tgttcccagg cgtccacgac
    17281 cagtttttcc accgccactt gctgactcat taaccctttc tgaaactgcg ggatatcgcc
    17341 gaccaccagt ggttcaggcc gtagtagcgc acgcgcatct gtttgcgatc ttgcagccca
    17401 acaatttgcg ccatccggct ttcaataccg attggcatgt cacagatgtt gaggattttc
    17461 gcattcgggc gcagacgacg cgtggcttct gcgacaattg ctgccgggtt ggagtagttg
    17521 agcatctagg cgtttggtga atatttttcc atataatcca ccagttccag gacgccgcca
    17581 atggaacgca tgccgtacgc tattccgccg ggtccgcaag tttcctgacc aacaacaccg
    17641 tggcgcagtg ggattttttc atccagttcg cgcatcgggt acttgccgac gcggatgtgc
    17701 gccatgacaa aatcaacgtc gctgaacgcc acttcaggat cggtggtgta actaaacgca
    17761 atgtccggcg ctttttcttt aaggatgact ttacaggctt cggcaatcac ttcctgccgc
    17821 gcgccatcgt tatcataaaa tttcagtgca cgaagcggga aacggtcctg attcgccagg
    17881 agcatcaaca cgatgcctgg cgtaaaggtg cttccaccgc ctgcgacaac cactgagaat
    17941 ttggtcatta tactgcctcc gtaatggcaa cattttctgc tgattgatga gaattaatta
    18001 agctatcgag ctgttcacgc aattgggata catgcagacc aatgatcacc tgaatggcat
    18061 cgccactacg gaagacgccg tgcgctccca gctttttaaa gacttcgtca tccagcgttt
    18121 gtgacatgtc atgcagtgca atacgtagac gcgtcgcgca attgttaatg ctggagatat
    18181 tgccgacccc gcccagggct tgcaggatac cggcagcctg atccagctct ttttttggct
    18241 ctgcagcggt ggtttggcct cgcgaggctt tgtattcggc ttttgagtag agtttcactt
    18301 ccgcatcttc acgtcccggc gtgcacatgt tgaactgcag aatcagggtg cggaaaacca
    18361 cgaagtacag cagggtaaag cacaacccaa tggcgatttg ggtcagcatc atatccgcgt
    18421 ggttgctgaa catcgggatc cagttttgcg gtaaaacctg gtcaatcaga cctccgccca
    18481 tgttgcccac cacaccaaag agatacatta cggtcgacat tgaggccgcc agcacggcgt
    18541 gtaccgcaaa cagcaacggt gaaatgaaca ggaaggtaaa ttccagcggt tcggta
//
LOCUS       DAZSNO010000070        18000 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZSNO010000070 DAZSNO010000000
VERSION     DAZSNO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18000
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.070"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..2264
                     /locus_tag="ACHWN2_002306"
                     /pseudo
     CDS             1..2264
                     /locus_tag="ACHWN2_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2265..3914
                     /locus_tag="ACHWN2_002307"
     CDS             2265..3914
                     /locus_tag="ACHWN2_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR8931820.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3919..4695
                     /locus_tag="ACHWN2_002308"
     CDS             3919..4695
                     /locus_tag="ACHWN2_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR8931821.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4970..7819
                     /gene="rcsC"
                     /locus_tag="ACHWN2_002309"
     CDS             4970..7819
                     /gene="rcsC"
                     /locus_tag="ACHWN2_002309"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR8931822.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACHWN2_002310"
     CDS             complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACHWN2_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR8931823.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACHWN2_002311"
     CDS             complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACHWN2_002311"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR8931824.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12096..13217
                     /gene="ompC"
                     /locus_tag="ACHWN2_002312"
     CDS             12096..13217
                     /gene="ompC"
                     /locus_tag="ACHWN2_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR8931825.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13329..14384
                     /gene="apbE"
                     /locus_tag="ACHWN2_002313"
     CDS             13329..14384
                     /gene="apbE"
                     /locus_tag="ACHWN2_002313"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR8931826.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14458..15522
                     /gene="ada"
                     /locus_tag="ACHWN2_002314"
     CDS             14458..15522
                     /gene="ada"
                     /locus_tag="ACHWN2_002314"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR8931827.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15522..16172
                     /gene="alkB"
                     /locus_tag="ACHWN2_002315"
     CDS             15522..16172
                     /gene="alkB"
                     /locus_tag="ACHWN2_002315"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR8931828.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            16248..17891
                     /gene="yojI"
                     /locus_tag="ACHWN2_002316"
     CDS             16248..17891
                     /gene="yojI"
                     /locus_tag="ACHWN2_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR8931829.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         4350 a         4382 c         4840 g         4428 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct atgaccaggc
      181 gctctccagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     1081 ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataatat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaaggggggg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtta gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaatgt gcaggtccgc tggcgtgaac cggcaaaaac ggctcaacaa agtaacattc
     1801 cggtgaccgt tgaacgccag ttgtatcggc ttatccccgg tgaagaagag atgagcttta
     1861 ctctgcagcc ggtgaccagc aatgagattg acagcgatgc gctgtatctc gatgaaatta
     1921 cgcttaccag cgagcaggat gcagttctgc gctatggtca ggtggaagta ccgctcccgc
     1981 cgggagctga cgttgagcgc acaacatggg gcatttcagt caataaaccc aacgccgcga
     2041 aacagcaggg gcaattgctg gaaaaagcgc gtaatgaaat gggcgaactg gcctatatgg
     2101 tgccggtgaa agaactgacg ggaacggtca ctttccgcca tttgctgcgc ttctcgcaaa
     2161 aagggcaatt cgttctgcct cctgctcgtt atgtgcgttc ctatgcacct gcgcagcaaa
     2221 gtgtcgcggc agggagtgaa tggaccggga tgcaggtgaa ataagtgaac tggcgcagaa
     2281 tcgtctggct attggcgttg gttactctgc caacgcttgc ggaggagacg ccgttacaac
     2341 tggtgctgcg cggtgcgcag cacgatcaac tgtatcagct gtcgtcatca ggcgtgacca
     2401 aagtctcagc attacctgac tcgctcacca cgccgttagg gagtttgtgg aaactgtatg
     2461 tttacgcctg gctggaggat acccatcaac cggagcagcc ctaccagtgt cgcggaaact
     2521 ccccggaaga ggtttattgt tgtcaggctg gggagagcat aacgcgcgat accgcgctgg
     2581 tacgttcctg tgggctctat tttgcgccgc agcggttgca tattggcgca gatgtgtggg
     2641 ggcaatactg gcaacagcgt caggcacctg cgtggttagc gtctcttaca acgctgaagc
     2701 cagaaacctc ggtaacggtt aaatcgctgc ttgattcatt agcgacgtta cctgcgcaaa
     2761 acaaagctca ggaagtctta ctggatgtgg tgctggacga ggcgaaaatc ggtgtcgcat
     2821 ccatgctcgg tagtcgggta cgggtgaaaa cctggagctg gtttgccgat gataaacagg
     2881 aaatccgtca gggtggtttt gctggctggt taaccgatgg tacaccgctg tgggttactg
     2941 gcagtggcac cagcaaaacg gtactaaccc gttatgcgac ggtgcttaat cgggttttgc
     3001 cagtgccgac gcaggttgcc agtgggcaat gtgtagaggt ggagctgttt gcccgctatc
     3061 cgctgaagaa aatcacagcg gaaaagagta cgacggcagt aaaacccagc gtacttaatg
     3121 ggcgctatcg tgtgacgttt actaatggta accacattac gtttgtcagc cacggtgaga
     3181 cgaccttact gagcgaaaag ggcaaactga aattgcagtc gcatctcgat cgtgaagagt
     3241 acgttgccag agttctggat cgcgaggcga aaagtacgcc acctgaggca gcgaaagcca
     3301 tgactgtggc gatacgtact tttttacagc aaaacgccaa tcgggaaggt gattgcctga
     3361 ccatccccga cagcagcgcc acgcagcgtg tttctgcttc gccagcgacc acgggggcgc
     3421 gaacgatgac cgcctggacg caggagctca tctatgcagg cgatccggtt cattatcatg
     3481 gcagtcgcgc taccgaaggg acactttcct ggcggcaagc cacggcccag gcggggcagg
     3541 gggagcgtta cgaccagata ctcgcgtttg cttatcccga caacagcctt agccgctggg
     3601 gagctccacg ctcaacctgc cagttattac ccaaagcgaa agcctggctg gcgaaaaaaa
     3661 tgccgcagtg gcggcgtata ttacaaggcg agacggggta caacgaacca gacgtgtttg
     3721 cggtctgtcg tttagtctca ggtttcccct ataccgatcg gcagcagaaa cggctgttta
     3781 ttcgtaactt ctttacgctt caggatcggc tggatttaac ccatgagtat ctgcacttgg
     3841 cctttgatgg ttatcccacc gggcttgatg agaactatat cgaaacgctg acccgacaat
     3901 tattaatgga ctaatgctat gcgaaagatt tttcttccgt tgttactggt ggcactttcg
     3961 cctgtcgctc acagtgaagg tgtgcaggaa gtcgagattg acgcaccgct ctctggctgg
     4021 catccggcag agggcgaaga tgccagtttt tcacaatcga tcaattaccc ggcgtcgtcc
     4081 gtcaacatgg cagatgatca aaatatctca gcgcagatcc gcggcaaaat aaagaattat
     4141 gctgcggcgg gtaaagttca gcagggccgg ctggtggtca acggtgccag tatgccgcag
     4201 agaattgaat ccgatggttc atttgcacgc ccttatattt tcactgaagg cagcaacagc
     4261 gtgcaggtca tcagcccgga tgggcaaagc cggcaaaaaa tgcagtttta ctcaacgccg
     4321 ggtacaggaa ctattcgtgc acgtttacgg ctggttctct cgtgggatac ggacaatacc
     4381 gatctcgatc tccatgttgt cacgcccgat ggcgaacacg cctggtacgg taacaccgtg
     4441 ctgaaaaaca gtggtgcact ggatatggat gtcacgacgg ggtacggacc cgagattttc
     4501 gccatgccag caccagttca cgggcgctat caggtgtata tcaactacta tggtgggcgc
     4561 agcgaaacgg aattgacgac cgcccaactg acgctgatca ccgatgaagg atcggtcaat
     4621 gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac gctggtgaaa
     4681 agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt cagataagac
     4741 tgctgggcga tctgccgacg ccaggatata accattaaca agcgtcggca gattggtcat
     4801 ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt aaggcaatgg
     4861 tttctaagcg atacgcatta ttgcaatggt gacagaacat tgcggtaaca cgcttttacc
     4921 gctaccttaa ccacactcca tcggtcacct gaggcggagc ttctcccctt tgaaatacct
     4981 tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat tggcgttagt
     5041 gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt tacatcagcg
     5101 agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc gctttattca
     5161 acgcacctct gatgtgatga aagagcttaa gtacatcgcc gaaaatcgct tatcggcaga
     5221 aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg cgtttgaacc
     5281 gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt ctctggagtc
     5341 attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg atctcaaccg
     5401 ggtattttta atcggcagtg ataacctctg catggccaat ttcggtctgc gtgatatgcc
     5461 agtggaacgc gataccgcgt tgaaagcgtt gcatgaacgc atcaataaat atcgaaatgc
     5521 accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc gcccttgcgt
     5581 cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg cgcttttggg
     5641 tgtcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc cgatgggggt
     5701 taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag aaagtaaaat
     5761 taagggcgat cctcgctgga tgcaggaacg ctcctggttt ggctatacgg aagggttccg
     5821 ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt attcggtgcc
     5881 ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt tgctgaatgt
     5941 gcttgccgga gctgcattgt ttactctcgc gcggatgtac gagcgacgta ttttcattcc
     6001 ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca agattgtcgc
     6061 ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata ttttaagtaa
     6121 cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac gactgacgca
     6181 aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca ataccaatct
     6241 gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt gtgtgctggt
     6301 ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac aagcagcgga
     6361 acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc tgcgaacgcc
     6421 gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac cgaaaggcgt
     6481 cgatcgtctg gtgacggcaa tgaacaactc ttccagcctg ttgttgaaaa ttatcagcga
     6541 tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc gtgagttttc
     6601 accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg tacgcaagca
     6661 gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg gcgacccgat
     6721 gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca ccgataccgg
     6781 ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc tctatccgtg ttcgcgatac
     6841 cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct tccaggttgg
     6901 aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt gtgaaaaact
     6961 gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg gcagccagtt
     7021 taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg tggaagggtt
     7081 gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt tcctggaaac
     7141 cagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac cgactcccga
     7201 agatgtgtta atcactgacg aggtagtgag taaaaaatgg cagggcagag cggtagtgac
     7261 cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt gggtacacag
     7321 tgtggctgct ccgcatgagc taccggcatt gttggcgcgt atttatttga tcgagatgga
     7381 gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg ataacgacga
     7441 tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg cagatcagtt
     7501 gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc gtcgatgcgc ttaatgtact
     7561 tagtaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata tggatggtta
     7621 ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg gagtaactgc
     7681 taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca gctgcctctc
     7741 gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg agagggtcag
     7801 gaaatcgcgg gattcgtagg acggataagg cgtttacgcc gcatccggca ttcagtgcaa
     7861 atgccagata agacactgac gcgtcttatc tggcctactt taatgtaatc tgttgaaata
     7921 atgggaatcg taggccggat aaggcgttta cgccgcatcc ggcaatcggt gcaaatgcca
     7981 gataagacac taacgcgtct tatctggcct acagatgatt agtctttatc tgccggactt
     8041 aaggtcacag aagagagata attcagcagg gcgatatcgt tctcgacgcc cagcttcatc
     8101 atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt tttagcgatc
     8161 tcggtcacta ggaagccttc cgcaaacagg cgcagaactt cactctcttt tggcgagaga
     8221 cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac gctttccggg
     8281 gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt cggtgcacct
     8341 tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat cgccgggttg
     8401 ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat gtacttgatt
     8461 aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat caacacatgc
     8521 gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc gccgacaaca
     8581 ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat cggatggtca
     8641 tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca gcaagctctt
     8701 gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct tctcacgaat
     8761 cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac
     8821 gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt
     8881 atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc
     8941 atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt cctgtgccag
     9001 ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac
     9061 gcacaattga ccaggcccaa tttctcgtac gccagactca tcatcgctta aaagcaagcc
     9121 agaggcagta agattagacg gattatccgt taaaaagata tcataatctt gactaattaa
     9181 tctttcatcg ggtgtgatac aggttgcacc ccaattttct aacaggcgag tgacaatatt
     9241 ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta aacgctcttc
     9301 ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt agcgtgtacc
     9361 aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgtgcca gttgatcgct
     9421 cagccagaat gccagcgggt ccgcctttcc ataacgatcg ttttgggtct ggttgataaa
     9481 cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt ccagaatgcg
     9541 gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga tttttcccaa
     9601 ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta aggcatcgcg
     9661 atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt
     9721 acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct gcacggagaa
     9781 cagcaccgtc tcacttttcc agctatcatc cgcaagcatg ttcgctaact gtatttcttc
     9841 gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg gggcgttgag
     9901 tttagccgca ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt
     9961 cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt
    10021 cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc
    10081 gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat
    10141 cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcagatgcg gcagcaaatg
    10201 atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc
    10261 gagcggcaac agtgagacta tctcttcatt gattgcccgt aaaatgcgca attcgttatt
    10321 gaccgccgtg ctggggacgc tttctgtact gcggctggag aaatggcgga atgtggtata
    10381 gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg
    10441 caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca tatcggtaga
    10501 gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt
    10561 cccttctttc tcattatcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact
    10621 gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt
    10681 tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac cgttctgcca
    10741 ggctaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg cctgttgtaa
    10801 catttctgca cgacgtgaat caacaatgtc actgacggtc gattctttaa atccggaggt
    10861 gagatcttta aggggtaggg ttgagatcag caccagactg ttatcctgac cattcaggta
    10921 atacatcgac cacggtacat tttctgcacc ccacaatgta tccagataag tggacatccg
    10981 ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt ccgttttgcg
    11041 gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc
    11101 ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc tgcctggtca cgtaacgcca
    11161 ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca atctggtaac ttttatcttt
    11221 cagccaagcg ttaacggcac tttgtaccat tacccccatc gtcaccagta acacaatgat
    11281 caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt
    11341 tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca
    11401 gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc
    11461 cgtgatattt atcatcctgt atggggggaa atagatgtac aagcgccatt ttgctgtaca
    11521 aataacctac aaaaagccca acgaaattaa ttgcaaataa acaaagattg ctggaaatta
    11581 tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct gtggtagcac
    11641 agaataatga aaagtgtgta aagaagggta aaaaaaccga atgcgaggca tccggttgaa
    11701 ataggggtaa acagacattc agaaatgaat gacggtaata aataaagtta atgatgatag
    11761 cgggagttat tctagttgcg agtgaaggtt ttgttttgac attcagtgct gtcaaatact
    11821 taagaataag ttattgattt taaccttgaa ttattattgc ttgatgttag gtgcttattt
    11881 cgccattccg caataatctt aaaaagttcc cttgtattta cattttgaaa catctatagc
    11941 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
    12001 tggggagaat ggacttgccg actgattaat gagggttaat cagtatgcag tggcataaaa
    12061 aagcaaataa aggcatataa cagagggtta ataacatgaa agttaaagta ctgtccctcc
    12121 tggtcccagc tctgctggta gcaggcgcag caaacgctgc tgaagtttac aacaaagacg
    12181 gcaacaaatt agatctgtac ggtaaagtag acggcctgca ctatttctct gacgacaagt
    12241 ctgtagatgg cgaccagacc tacatgcgtc ttggcttcaa aggtgaaact caggttactg
    12301 accagctgac cggttacggc cagtgggaat atcagatcca gggcaacagc gctgaaaacg
    12361 aaaacaactc ctggacccgt gtggcattcg caggtctgaa attccaggat gtaggttctt
    12421 tcgactacgg tcgtaactac ggcgtagttt acgacgtaac ttcctggacc gacgtactgc
    12481 cagaattcgg tggtgacact tacggttctg acaacttcat gcagcagcgt ggtaacggct
    12541 tcgcaaccta ccgtagcacc gacttctttg gtctggttga cggtctgaac ttcgctgttc
    12601 agtatcaggg caaaaacggt agcccggaag gtgaaggcat gaccaacaac ggtcgtgaag
    12661 ctctgcgtca gaacggtgac ggcgttggcg gttctattac ttatgattac gaaggcttcg
    12721 gtatcggtgc tgcagtttcc agctccaaac gtactgatga ccagaacttc ggtctgaacc
    12781 gttatgatga gcgttacatc ggtaatggcg accgcgctga aacctacacc ggtggtctga
    12841 aatacgacgc gaacaacatc tacctagctg ctcagtacac ccagacctac aacgcaactc
    12901 gcgtaggcaa cttgggttgg gcgaacaaag cacagaactt cgaagctgtt gctcagtacc
    12961 agttcgactt cggtctgcgt ccgtccctgg cttacctgca gtctaaaggt aaaaacctgg
    13021 gtgtcatcaa tggtcgtaac tacgacgacg aagatatcct gaaatatgtt gatgttggtg
    13081 cgacctacta cttcaacaaa aacatgtcca cctacgttga ctacaaaatc aacctgctgg
    13141 acgacaacca gttcactcgt gacgctggca tcaacactga taacatcgta gctctgggtc
    13201 tggtttacca gttctaatct cgatggatat cgaacaaagg gcctgcgggc ccttttttca
    13261 ttgttttcag cgtacaaaca cagttttttg gtgtactctt gcgaccgttc gcatgaggat
    13321 aatcacgtat ggaaataagc tttacccgcg tggcactgct ggttgccgcg ctcttctttg
    13381 ttggttgcga tcaaaaacca caacccgcca aaacccacgc tactgaagtt accgttcttg
    13441 aaggcaaaac catgggtacc ttctggcgtg ccagcatccc gggcattgat gccaaacgca
    13501 gtgccgaact taaagaaaag attcagaccc agctggacgc cgacgatcag ctgctttcga
    13561 cctataaaaa agattccgcg ctgatgcgct ttaacgactc gcaaagtttg tcgccgtggc
    13621 cggtaagtga agcgatggcc gatatcgtca ccacctcgct gcgcattggg gcaaggaccg
    13681 atggtgcgat ggatattacc gtcgggccgc tggtgaatct gtggggcttt ggcccggaac
    13741 aacagccggt tcaaattccg agccaggaac agatcgatgc gatgaaagcc aaaaccggct
    13801 tacagcacct gacggtcatc aatcagtcgc atcagcaata tctgcaaaaa gacctgccgg
    13861 atttatatgt cgatctctcc accgtcggtg aaggttatgc ggcggatcac ctggcacgct
    13921 tgatggagca ggaagggatt tcccgctatc tggtgtcggt gggcggcgcg ctgaacagcc
    13981 gtggtatgaa cggtgaaggc cagccgtggc gggtagcgat tcaaaaacca accgataaag
    14041 aaaacgcggt tcaggctgtg gtggatatca acggtcatgg gattagcacc tctggcagct
    14101 accgtaacta ttacgaactg gacggcaaac gtctttccca tgttatcgat ccgcaaaccg
    14161 ggcgtcccat cgaacacaat ctggtatccg tgacggtgat tgctccgacg gcgctggaag
    14221 ccgatgcctg ggatactggc ttgatggtac tcgggccgga gaaagccaaa gaggttgttc
    14281 gccgggaagg gctggcggtt tatatgatca ccaaagaagg cgatagcttt aaaacctgga
    14341 tgtcaccaca gtttaaaagc ttccttgtca gcgaaaaaaa ttaaagcgca agattgttgg
    14401 tttttgcgtg atggtgaccg ggcagactaa aggctatcct taaccaggga gctgattatg
    14461 aaaaacgcca catgcttaac tgacgatcaa cgctggcaat ctgtcttagc ccgcgacccg
    14521 aatgccgacg gcgaattcgt tttcgccgtg cgcaccacgg gtatcttttg ccgtccttct
    14581 tgccgcgcca gacatgcctt gcgggaaaat gtctccttct acgcaaatgc cagcgaggca
    14641 ctcgccgccg gatttcgccc ctgcaaacgt tgtcagccag acaaagccaa tccccggcaa
    14701 catcggttgg ataaaatcac ccacgcgtgt cgactgctgg aacaggaaac gcctgtaacg
    14761 ctggaagcct tagccgacca ggtggcgatg agtccatttc atctacatcg gttgtttaaa
    14821 gctactaccg gaatgacgcc taaagcctgg caacaggcct ggcgcgctcg ccgtttgcgc
    14881 gaatcgctgg cgaaagggga gagcgtgacg acgtctattc ttaacgccgg attccccgac
    14941 agcagcagtt actaccgcaa agccgatgaa acgctgggca tgacggctaa acaattccgt
    15001 cacggtggcg aaaatctggc ggtgcgttac gcgctggctg attgtgagct gggtcgttgc
    15061 ctggtggcag aaagcgagcg ggggatttgc gcgatattgc tgggcgatga tgacgcgaca
    15121 ttaatcagcg agttgcagca gatgtttccc gctgccgaca acgcgcctgc cgatctgacg
    15181 tttcagcaac atgtgcgtga agtgatcgcc agcctcaatc aacgcgatac gccgctgacg
    15241 ttaccgctgg atattcgcgg cactgctttt cagcaacaag tctggcaggc actgcgcacg
    15301 ataccttgtg gtgaaaccgt cagttatcag caactggcta acgccatcgg caaaccgaaa
    15361 gcggtacggg ccgttgccag cgcctgtgcc gccaacaagc tggctatcgt aataccctgt
    15421 catcgggtgg tccgtggtga tggcacactt tccggttacc gctggggcgt gtcgcgtaaa
    15481 gcgcaactgc tgcgccgcga agctgaaaat gaggagaggt aatgttggat ctgtttgccg
    15541 atgctgaacc gtggcaagag ccactggcgg ctggtgcggt aattttacgg cgttttgctt
    15601 ttaatgctgc ggagcaacta atccgcgata ttaatgacgt tgccagccag tcgccgtttc
    15661 gccagatggt cgctccaggc ggatatacca tgtcggtggc gatgaccaac tgtgggcatc
    15721 tgggctggac gacccatcgg caaggttatc tctattcgcc cattgatccg caaacaaata
    15781 aaccgtggcc cgctatgcca caaagctttc atgatttatg tcaacgtgcg gctacggcgg
    15841 cgggctatcc agatttccag ccagatgctt gtcttatcaa ccgctacgct cctggcgcga
    15901 aactgtcgct gcatcaggat aaagacgaac cggatctgcg cgcgccaatt gtttctgttt
    15961 ctctgggctt acccgcgatt tttcaatttg gtggcctgaa acgtaatgat ccgctcaaac
    16021 gtttgttgtt ggaacatggc gatgtggtgg tatggggcgg tgaatcgcgg ctgttttatc
    16081 acggtattca accgttgaaa gcggggtttc atccactcac caccgactgc cgctacaacc
    16141 tgacattccg tcaggcaggt aaaaaagaat aaaaataaga attattattg ctgtgcgcgc
    16201 gaagatgttt aaactgcggg ctgtaattca ttgtccgggt tttctgcatg gaacttcttg
    16261 tacttgtctg gcggcagtat cgctggccat ttatcagtgt gatggcgcta agcctcgcca
    16321 gtgcggcatt aggcattggc ttaattgctt ttatcaatca gcgccttatc gaaacggcgg
    16381 ataccagtct gctggtgtta ccggagtttc tgggattatt gctgctgttg atggcagtca
    16441 ctctcggatc gcaactggcg ctcaccactt tggggcatca cttcgtttac cgactgcgta
    16501 gtgaatttat caagcggatt ctggataccc acgtcgagcg cattgaacaa ctcggtagcg
    16561 cctcgttgct ggcgggatta accagcgatg tgcgcaatat caccattgct tttgtgcgtc
    16621 tgccggaact ggggcagggg atcattctca ctatcggttc atcggcgtat ctgtggatgc
    16681 tgtcgggcaa aatgttgctg gtaacggcta tctggatggc gatcaccatc tggggcggtt
    16741 ttgtgctggt ggcgcgggtg tacaaacata tggcgaccct acgtgaaacc gaagacaagc
    16801 tgtacacgga ttttcaaact gtacttgaag ggcgcaaaga gctgactctg aaccgggaac
    16861 gcgccgagta tgtgtttaac aacctctaca tccccgatgc gcaagagtat cgccatcata
    16921 ttattcgcgc agacaccttc catcttagtg ccgtgaactg gtcaaacatc atgatgctgg
    16981 gcgctatcgg cctggtgttc tggatggcga acagtctcgg ttgggctgat accaacgttg
    17041 ctgcgaccta ttcgttgacg ctgttattcc tgcgtacgcc gctgctttcg gcggttggcg
    17101 cattgccgac gctgctgacg gcgcaggtgg cgtttaacaa gctgaacaaa ttcgcgctcg
    17161 cgcctttcaa agctgagttt ccgcgcccgc aggcgtttcc caactggcaa acgctggagc
    17221 tgcgtaacgt gacgtttgct tatcaggata acgcgttttc cgttggtccg attaatctca
    17281 ccatcaaacg tggcgagctg ctgtttctga ttggcggtaa cggtagcgga aaatcgacgc
    17341 tggcgatgtt gttgacgggc ttgtatcagc cacaaagcgg cgaaatcttg ctggatggca
    17401 aacctgtcag cggcgaacaa ccggaagatt atcgcaaact gttttcggca gtgtttaccg
    17461 atgtctggct gtttgatcaa ctgctggggc cggaggggca acccgctaac ccgcaactgg
    17521 ttgagaagtg gctggcgcag ctgaaaatgg ctcataagct tgagttaagc aacgggcgta
    17581 ttgttaacct gaagttatca aaagggcaga aaaaacgcgt ggcgctgttg ctggcgctgg
    17641 cagaagaacg cgatattatc ctgctggatg aatgggcggt ggatcaggat ccacacttcc
    17701 gtcgtgagtt ttatcaggtg ttgctgccgc tgatgcagga gatgggtaaa actattttcg
    17761 ctatcagtca tgatgatcat tactttatcc acgccgaccg cctgctggaa atgcgcaatg
    17821 ggcaacttag cgagctgacg ggcgaagagc gcgatgccgc ttcgcgtgat gccgttgccc
    17881 ggacggcata atattacgac gctgacctgg cggactgctg ccgtcaggtc aatatgctaa
    17941 aaatcccttc atttctaata accctataat taatcaacga aattataatg tttctaaaat
//
LOCUS       DAZSNO010000071        17924 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZSNO010000071 DAZSNO010000000
VERSION     DAZSNO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17924
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.071"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHWN2_002317"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHWN2_002317"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR8931830.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="ACHWN2_002318"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="ACHWN2_002318"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR8931831.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHWN2_002319"
     CDS             complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHWN2_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR8931832.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHWN2_002320"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHWN2_002320"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR8931833.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="ACHWN2_002321"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="ACHWN2_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHWN2_002322"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHWN2_002322"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR8931834.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHWN2_002323"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHWN2_002323"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR8931835.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHWN2_002324"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHWN2_002324"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR8931836.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHWN2_002325"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHWN2_002325"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR8931837.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHWN2_002326"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHWN2_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR8931838.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHWN2_002327"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHWN2_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR8931839.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHWN2_002328"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHWN2_002328"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR8931840.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHWN2_002329"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHWN2_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR8931841.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHWN2_002330"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHWN2_002330"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR8931842.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHWN2_002331"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHWN2_002331"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR8931843.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHWN2_002332"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHWN2_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR8931844.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
BASE COUNT         4189 a         4556 c         4814 g         4365 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctca
//
LOCUS       DAZSNO010000072        17864 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZSNO010000072 DAZSNO010000000
VERSION     DAZSNO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17864
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.072"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..613)
                     /locus_tag="ACHWN2_002333"
     CDS             complement(<1..613)
                     /locus_tag="ACHWN2_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="TraN; TrhN; involved in stabilizing mating pairs
                     during plasmid conjugation; F plasmid TraN appears to
                     recognize OmpA in the recipient cell; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFR8931845.1"
                     /translation="MKEKINTFFRSLNFNYSVKPCNLCAIVFFLSFSVFADSNSDYRA
                     GSDFAHQIKGQGSSSIQGFKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWA
                     TGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEF
                     TNYTCERDTMVEEYCTRTASITGDWKYTDEYREVTIPHSQFRFS"
     gene            complement(849..1229)
                     /locus_tag="ACHWN2_002334"
     CDS             complement(849..1229)
                     /locus_tag="ACHWN2_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931846.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(1226..1864)
                     /gene="trbC"
                     /locus_tag="ACHWN2_002335"
     CDS             complement(1226..1864)
                     /gene="trbC"
                     /locus_tag="ACHWN2_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFR8931847.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(1873..2178)
                     /locus_tag="ACHWN2_002336"
     CDS             complement(1873..2178)
                     /locus_tag="ACHWN2_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931848.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(2208..3200)
                     /gene="traU"
                     /locus_tag="ACHWN2_002337"
     CDS             complement(2208..3200)
                     /gene="traU"
                     /locus_tag="ACHWN2_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFR8931849.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(3197..3829)
                     /gene="traW"
                     /locus_tag="ACHWN2_002338"
     CDS             complement(3197..3829)
                     /gene="traW"
                     /locus_tag="ACHWN2_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFR8931850.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(3826..4212)
                     /gene="trbI"
                     /locus_tag="ACHWN2_002339"
     CDS             complement(3826..4212)
                     /gene="trbI"
                     /locus_tag="ACHWN2_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR8931851.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(4209..6839)
                     /gene="traC"
                     /locus_tag="ACHWN2_002340"
     CDS             complement(4209..6839)
                     /gene="traC"
                     /locus_tag="ACHWN2_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFR8931852.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(6965..7312)
                     /locus_tag="ACHWN2_002341"
     CDS             complement(6965..7312)
                     /locus_tag="ACHWN2_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931853.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(7340..7558)
                     /locus_tag="ACHWN2_002342"
     CDS             complement(7340..7558)
                     /locus_tag="ACHWN2_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931854.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(7657..8130)
                     /locus_tag="ACHWN2_002343"
     CDS             complement(7657..8130)
                     /locus_tag="ACHWN2_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931855.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(8123..8536)
                     /locus_tag="ACHWN2_002344"
     CDS             complement(8123..8536)
                     /locus_tag="ACHWN2_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931856.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(8529..8750)
                     /gene="traR"
                     /locus_tag="ACHWN2_002345"
     CDS             complement(8529..8750)
                     /gene="traR"
                     /locus_tag="ACHWN2_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFR8931857.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(8885..9400)
                     /gene="traV"
                     /locus_tag="ACHWN2_002346"
     CDS             complement(8885..9400)
                     /gene="traV"
                     /locus_tag="ACHWN2_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFR8931858.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(9397..9717)
                     /locus_tag="ACHWN2_002347"
     CDS             complement(9397..9717)
                     /locus_tag="ACHWN2_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFR8931859.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(9704..10294)
                     /gene="traP"
                     /locus_tag="ACHWN2_002348"
     CDS             complement(9704..10294)
                     /gene="traP"
                     /locus_tag="ACHWN2_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFR8931860.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(10284..11711)
                     /gene="traB"
                     /locus_tag="ACHWN2_002349"
     CDS             complement(10284..11711)
                     /gene="traB"
                     /locus_tag="ACHWN2_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFR8931861.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(11711..12439)
                     /gene="traK"
                     /locus_tag="ACHWN2_002350"
     CDS             complement(11711..12439)
                     /gene="traK"
                     /locus_tag="ACHWN2_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFR8931862.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(12426..12992)
                     /gene="traE"
                     /locus_tag="ACHWN2_002351"
     CDS             complement(12426..12992)
                     /gene="traE"
                     /locus_tag="ACHWN2_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFR8931863.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(13014..13325)
                     /gene="traL"
                     /locus_tag="ACHWN2_002352"
     CDS             complement(13014..13325)
                     /gene="traL"
                     /locus_tag="ACHWN2_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR8931864.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(13340..13699)
                     /gene="traA"
                     /locus_tag="ACHWN2_002353"
     CDS             complement(13340..13699)
                     /gene="traA"
                     /locus_tag="ACHWN2_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR8931865.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(13733..13960)
                     /gene="traY"
                     /locus_tag="ACHWN2_002354"
     CDS             complement(13733..13960)
                     /gene="traY"
                     /locus_tag="ACHWN2_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFR8931866.1"
                     /translation="MIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            complement(14080..14727)
                     /locus_tag="ACHWN2_002355"
     CDS             complement(14080..14727)
                     /locus_tag="ACHWN2_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TraJ family protein"
                     /protein_id="HFR8931867.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFSCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(14919..15302)
                     /gene="traM"
                     /locus_tag="ACHWN2_002356"
     CDS             complement(14919..15302)
                     /gene="traM"
                     /locus_tag="ACHWN2_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFR8931868.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            15635..16225
                     /locus_tag="ACHWN2_002357"
     CDS             15635..16225
                     /locus_tag="ACHWN2_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFR8931869.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(16522..17343)
                     /locus_tag="ACHWN2_002358"
     CDS             complement(16522..17343)
                     /locus_tag="ACHWN2_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR8931870.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(17454..17750)
                     /locus_tag="ACHWN2_002359"
     CDS             complement(17454..17750)
                     /locus_tag="ACHWN2_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931871.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            complement(17774..>17864)
                     /locus_tag="ACHWN2_002360"
     CDS             complement(17774..>17864)
                     /locus_tag="ACHWN2_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR8931872.1"
                     /translation="VMVNTGGNKRQVPAKRQNRGSRNSTDDGG"
BASE COUNT         4511 a         4705 c         4035 g         4613 t
ORIGIN      
        1 tactgaaccg gaactgtgag tgcggtatcg tcacttcacg atattcatcc gtgtatttcc
       61 agtccccggt gatactggca gtccgtgtgc agtactcctc caccatcgtg tcccgctcac
      121 aggtatagtt tgtgaattca ctgcggttta tctcctgcgc actgcactgc tgtccggtat
      181 ttcccacaat gctgtcagcc ctgttcacca catccctgcc ggtctgaatg aacggtgcat
      241 ccggtgacag aatgtctttc ggcttgttca tgaaggactc tgtgatggtt tttccggttt
      301 caccggtcgc ccattccgtg gtgccgtcat ttttcaggcc gccatccccg ccggcagtca
      361 cgccaccgta atattttgtc tcgtccggat tcgcgttata gccggggata ctctcctgtg
      421 gcttaaagcc ctgaatactg ctgcttccct gccctttgat ctggtgagca aaatcagaac
      481 cggcccggta atcactgttg ctgtcagcaa acacagaaaa gctcagaaaa aaaacaattg
      541 cacaaagatt gcacggcttt acggaataat taaaattcag agatctgaaa aaggtattta
      601 tcttttcttt cataacctga accatactgg agaatgaagt gatgtcatct caacttcaga
      661 actgtgttga ttgtggtaaa gcattaagtc gaacagccac aacgtgcggg aattgcagct
      721 caacggaccc ttttggaaaa aaacggcgca gcgatcgctt ttaccagtgg tttgtagggc
      781 tgtttattac ggctattatt attgctggcg gattatggta catggggctt cttgacgtac
      841 cgggattact actcagataa cgacttcagt aacgatatca gtgttctgaa atcggcacat
      901 aacgagtgac gacaataccg gttataccag taatgagggg tgtcgggctg aagcctgaaa
      961 cagagctgaa acttactgtt aaaagccccc ttcaccgacc atccccatgc cagacgtggt
     1021 attgctgatt cagcatcctg ccgcagtcgc tgctcaagaa gggcagccat ttttaccgga
     1081 caggcaaagg caatataagg aggcagcttt gtaacaggga aaccatgtcc ctgacaggaa
     1141 gcatatgtgc ggaagccgtg aatgttcatc gcggccacca gggcacggat ctccggatca
     1201 atctgataat acccggcagg taaagtcatt tttgtttatc tcccgggtta ttcagcaacg
     1261 cccgagccac ctgccggcag tcccccgtgg cggccacttt ctccagcgcc tggccgaccc
     1321 gcaggttacc tcggataatg tcgtatccct gactgcagaa caccaccagt gccggtacac
     1381 tgcgaatgcc gtactgtgaa aacagcgtcg ggtcgatctg aacaccatca gtcgcgccgt
     1441 ctttcaccag ggacagcacg gcttcagcgg tggtcttcag gtcattattc accatgcccc
     1501 gcagtgtggc gggtataccg tagtgccggg tttcacccag catccgtttc agcccctctt
     1561 cgggaatgga aaacgacaca aaatacaggg caccctgccg gggtttcccg tcctgcgaag
     1621 cctgctgttt acgaaccagc tcatccagaa aatggttatc tgaacgctga agggggtttt
     1681 ccagcacctg tttctccgcc caggctttca gctgatggtc aggtttctct cgcagttgtc
     1741 tgcttaaatt ttcctgctgc ttcaggaact ggcggttttc aggtgtgttc acattttctg
     1801 aagcatgaac agcaagatgc atccctgctg tcagagcaaa aagtgcaaat attttccggt
     1861 tcatgatatt tctcactttc tgctatttac aggcatgaac tcatcagaaa aaagataatt
     1921 gagaaaaaca gccataaacg cacatgtaaa tgtcaccttc agatataatc tgacaagtgt
     1981 cacttgttct gatatatctg ccttcttcca ttcatgaata atttcagcga gaaaactgtc
     2041 aggtgcaaaa taaaataata taacgggcac cattacaaga ccaaaaggaa ataattttaa
     2101 tgttctgctt atcagcctga gcagcacagc acttattttc ctgaggtgct gaatatattt
     2161 actgtccatt gttgccattt tcatttcctc ttattcatta aaatgattta caggaataca
     2221 cagttacgtt tacgccacat caggtagccg aagtttttct ttgtgttggg cggatttttt
     2281 cccgtctccc agcgcatcac actgcgcccg aacggatggc actgcccgct gtccggatac
     2341 atattcacca tctgataacg ccagcgttct ttgggcagga ttggggacgg atattcatta
     2401 cagacggcgt tatttttccc gatggtttcc ataatcatgc cctgacggtg tagtttgaac
     2461 gccatgcgtt cactgaccag cagggaggac tgcaacggac tggactcatt actcacccag
     2521 ccattgaacg ggtacatact tccctgcgaa ccggcacacc agaacagaac atcgagaggc
     2581 atattaaagg cgctggcaat ggcatctgct gcacaggccc cctgtgctat cggattggca
     2641 aagatgacag cttccggatt gagaatggtg gtcaggctgc tgtccgtcca ggtggggtca
     2701 atttcagaaa gataagcgat atccaggtca ccaccttcca gacagcccag cgatgtgatg
     2761 atgttcagcc agtacgtcag cgggtatttg taccagtgaa cgtgatagaa cgccccgttc
     2821 acctgcttct catccttttt cgccgttccc tgtgccgttt taccaaaagc cggcaggctg
     2881 aagcccaggt taaccatgca tcccggtgac cgggtgacgt ccgtcagcgc catcggctcc
     2941 cagtaaccaa tggccagccc gatacgcctg aacagcggcg gcggtgccgg acaaatctga
     3001 atgggcatcg acgggttcgc cgtgtcgggg accttaccct gactgacttt gacactgccc
     3061 agcgagagcg ggaaaataca gctccagcag atatccgtga tcgggtttac aaaacgcccc
     3121 tcacaggcag aatccgcaga cgctgcaggg acatgaccgg cgagaaggaa taacatcagc
     3181 agccacaggc ttcgcttcat tttctgccct cctctgccgg aataaattcc accttcagga
     3241 agcgatcgcc gggaacggcg ctcacccgtg ccgggacctg atcaatgccg aggcgctggc
     3301 agagcacacc gttctggtca aagtaaacac ggctgtccag agacttctgc atctccggga
     3361 tactgccctg cacgaggata attttgctct ccagtgtggg cggcgtctgg cgtttcatcc
     3421 aggccacctg cgccggatca tcgccattga tgaaatacag cgtctggtta aaaggcacat
     3481 actgcagcgg attcatcact tcaccctgac gggcaaacac ccgcccttcg ttatcccgga
     3541 tatccgcagc cagtctgacg gacgggtcaa acaaccggct gccgtatttc tccgtgcgtc
     3601 cgataccggg cacggcaggg ggccgcagac tgttgcggat cacccgctcc ttaaaggcat
     3661 ccattttccg gcccatctca ccggactgct ccagtgccgt caggcgctgc atgatcaccg
     3721 tcagcatgtc cggctccttt accggccaca gatcgcccca ggtgccaaga tcggcggcgg
     3781 ccacactctg cccccatatc agcagggcaa tcagcccccg gcatctcatg gttccgctct
     3841 cattcgccgg gcaatatcct gctggatttc ccgggtgata tccggagccc cctgtaccac
     3901 ggcaggcgac accagaatga ccgcgtggtg tttctgctgc cacgcctgca ggctggcttc
     3961 cagtgccgtg ttaaaacgcg ctgaaagggc ttttgactgt gcttctgaca gttgtttctg
     4021 actggcgctg tcaaaaaatg catccacagt ctgtttcatg ttgaaggcgg cagtcaccgg
     4081 tgcgttcagt ctgacaatgc cgtaactgac ggctgcgttc agtaacacca ttgccaggca
     4141 tcccggcacc gtccaccacc agtggctgcg tcggggggct gtaacatcag tcgttttttg
     4201 cgttgtggtc atgcaacact cctgtatttc tcatgttctt ccagccaggc ttccagcgaa
     4261 gccatctccg gccctgactt cttccacgcc agctgccaca ccgcctcatg aatactcagg
     4321 ccctccctgc gtttctgctg cacaaactca aaatccgggc cgtcagagct gtacatggcg
     4381 cggcttaacg ggtccacaaa cagacggtgc caggaggaat ggttttccac ctgcagcagg
     4441 aaggaactga accactggtc tttggccgca ccaaacttgc caatcatgtc gcgctgcagt
     4501 ggctggaact gatccgggaa taactggtta tatttcgcga actccttcgc actctgtttg
     4561 aggataattt tgtaggagga gttaccccat gccgcacggg ccgcactgga ggccttgtca
     4621 gagtcaaagt cgacgatgtt ctgtgtgatg gtgatatagg caccggtatg gcggcgacag
     4681 gtgcggtaac ctttttgaat aaactcaccg accttgcgat ttttgaagtc cagcagacgc
     4741 cagccttcat caatgacgtt cagtttcttg agagtacgcg gcgtgcggta catcctgttc
     4801 tcgatgtaga taatcaggga gaacatcacc gcaaccagca gtgacggacg gtcttccagt
     4861 ccgcccagct ccagcaccac cattttcgca tcatcccgca gggacggctc atcggagtta
     4921 aaatactggc cgtaagtgcc gttggcagtg tactggtcaa gcagcacaat catttcgtcc
     4981 agacggctgc ggatggtcgg tgacccggca tactgctcgc tgtcactggc gtttttcagg
     5041 aaatccacca cgtcatcaat acgtgccctg ttctctttgg ccagccagga ggccctgacc
     5101 gcctgcagca gcagaccttc atgcacttca tccaggttac cgttggggct ggccatcact
     5161 gacaactggt cacggacacg ctccgctgac tggtcaatat cggtgatgtt cgcaaacggg
     5221 ttaaaacgca gggtttcacc gtccagatat acgcccccca tgttctcaca cagggacttg
     5281 tagccatccc ccatgtcgaa caccacggca aagcccccgg agtccagcac gctgcggata
     5341 agtggctgta tcagtccggt tttaccggcc ccggaggtgc cacagaccgc catgttgtag
     5401 ttggtgttat tcatcccctt aaagaagata tcgataaacg ccagctggtt acggtaggtg
     5461 ggcgccagca gacctgccgg tgtcaggggg ttatccgcca ctaacggcat caggttggcc
     5521 acattaaagc tctctgcgcg ctgaaccaca ccggcctctt tcagctgttt aaacagccct
     5581 ttcccggcca taaagggcag gcaggtcagg aaattgcgca tgtggttaaa gcgcggtgaa
     5641 atcagctcaa aaccgttttt acggaagctg ttcaggatgt cctgctccac ttccagtgcc
     5701 gtttcattat tgtccttgca gaaggctgtg atgttgagga agtaggacac gacagaagac
     5761 tggccggagc ccagccgctg gcgcagttcc ccccactcct tcgcctcttt ctccacggac
     5821 ggaaaccatt tggcatatga ggtcttcgac ttcttctcca ggtccatgta cttcaggttg
     5881 gcttcgctgt gggttttcac ctggtcttcc accaccagag tcagcgtcag gatgaacggg
     5941 caggaaatgg acatttccgg gttcagcaga ttgctgtagt tgtcggccat gttccacagg
     6001 aaggcgattt ccgggttacg ggccagatgg aaattcagga tgcgggccgt gctgttcctg
     6061 ccgttctcac gcaggcccag cgtcaggtaa tcagcccgga cattcaggtc aaaactgtcc
     6121 tccacacact gataattcag atcagaatac gggtccagct gacgtctttt cgggtacaga
     6181 gagtccggat tatggttaat catctccccg acaatctcga tgaaagcctg tgcatccact
     6241 gtctgtgtgg tgatttttgc gccgtgaaaa gaggcacgga tgattttcac cagattttcc
     6301 atttccagaa tgtcagcccg gctttttttc ttcgagggag aacagtacga gataaacacc
     6361 cggtaatgac gcagggtcag gggcagattc atcccctccg gcagcgggaa ctgtgtcgct
     6421 gccgctttca tataataggc acgggtaatg gcgttaaacc gttcagcctg ttcacctgac
     6481 caggagaact cacgcagccc gtattcaatc ctgtcaccaa ccagctgact ggacatcaga
     6541 tggatacaca acggaatacc gcgcggcagt ttggtgcgca gcatgtgatc gagtgcctcc
     6601 acaatggact tattcgcccc attgatggga atggcttcca gcataaagcc catcgtggtg
     6661 tcattcatga acagaccgga ttcctggttg taatcacggt acggcagcag acggctgaac
     6721 tgcgggaagc tcatttcgcc cagaacttca ttggcctttg cggattcgtc aggcagtttc
     6781 agtgctgtga cgagggagtt aaccgcctga gtgacggcct caagtgggtt attactcata
     6841 tgagtattcc ttagtgcgtt taaaaaagga aaatatcagt ctgtggccgt ggatatatca
     6901 cgcagtgcca ttcctgaaac cacagcacaa ataagagatg gaggctttta acaggaaacc
     6961 aggattaacg ttcctttact gaatgagcat catttaatgc ctgaatttgt ttttcgcgaa
     7021 tcttattgcg ctcattttct cttacccgtt tcacctctgc catttcgttt ctttttctgc
     7081 attctgatgc gattttattt gccttaccaa gtgttaaagg ttcagactga taattaagtg
     7141 tctcctgaaa tgcttctgct gcacagggag tttcctgtaa aatctctgtc agttgttctg
     7201 gtgttgcagg ttgttgcgca aggtaaacga tgtaaaaagg aatacataca atcatagcca
     7261 taacgccaac aaatataaaa gccagaacag attttaaata tatcgacttc atttcatttt
     7321 ccttcgaagc agtaagcact cattgcccac caccatttag tgtttccatc acgctgttga
     7381 aaggttcatt tgttttaact tcaatacact cccctgtatt tccaaagccc ctgccgccat
     7441 gtgcttcgat tgtccgaaga acctgcacgc atacgtccgt atttggcatg ggtgtaatat
     7501 tccagcttcc gccaccgtta atatttttta tcagcacaag aaaaataaag gtatgcattc
     7561 tttattatct ccgcttgtat attaatcact gaatatccct tcagttaact tatgaggtca
     7621 taaaacactg cagattttca ttcagattta ctctcttcag caaactttac tctcttcagc
     7681 aaacttccgg atggtatcct gttcacgctg acgggtggca gactcctgca ttgcatccac
     7741 tttctcctgc cagagacgcc ggatatcctt ttcatcccga ccagtcagtt ttttaccgga
     7801 cagcagacgg ttaagcagtt cctgtctcgc atccggtacg tcttcagtca gacgcataag
     7861 ctgaagcagg ctctgacggc tgagaaccat gagtgattcc ggcaggaggg tgaagaagaa
     7921 aataaaaaag cataccgcac cacagcaggc aaggaccgca aacaggattt tcagatatat
     7981 accatcaacg ccgaacgcag caataataca gggaatccat gcaatacacg cacaatacgc
     8041 caggatgtta tctattcggt tatgtctgcg atatatctgt tgatgttcat ccagaataga
     8101 atctattttt tcagcttctg atttatgcat agtgtttcct ctgcgtttcc tgccagagac
     8161 gccggatatc ctgttcatcc cgaccagtca gttttttacc ggacaacaaa cggttaagca
     8221 gttcctgtct cacatctggt acgtcttcag tcagacgcat aagccggagt aattcctgac
     8281 gactgagtac catgcgtgat tcctccggta cgctgtaata attaaaaata cgactgaata
     8341 ccccacaaca gcaggcaacg gacaggcaca gggatttcag atacaggcca gaaacacaga
     8401 atgtggcaac accacaaaga atagctataa ctaaaaatcc cttacccagt gccatacaca
     8461 aagaaccctg ttttctggat atccgctcat gctcatccag aataaaatct attttttcat
     8521 tttctgattt atgcatagtg tttcctctgc ctttcctgat acgcctgaca ttcaacgcac
     8581 aacgtcacac cgggaaatat tttccgccgg gcttccggaa taggatttcc gcatgcttca
     8641 cagagataaa caggaatacc atgaacattt atttcctggc gtatccggtt tattcctgtc
     8701 atggtcagtt gttctgtcac tgaatatgct tcatcggctt catcactcac ggaatattcc
     8761 tcccgacatg ttctgacacg ctaccgccca tcccttttca cgcacagggc actcgtttta
     8821 caggcggatt ttgaatacag aaagcccgcc ggcattgccg gctgatatac aacttcaggg
     8881 ccagttaatt aatacgtggt tttccccacg cagacggttt aataacaaaa aacacgctgg
     8941 atggctgatg ataaacatcc tggttatcaa tataaggcgc tatccataat gctgccgtct
     9001 gctcacccgt tcttagcgga cgaggaggtg ttaccggcgt aactgaactg accggaacaa
     9061 ccgttttcac ttcccgggca gcggtaaaca gcgggcgtgg tgccagcagt ttctgctccg
     9121 gatgtgctgt caccgcaggt ctgctgccgg acggagtggt cgcggtgacg gtctgcaccg
     9181 gcattgtccg gaagttccct tcagccaggc gcggcagtga tgccgcaacc ggctttgctt
     9241 ctgaggactg ctccagtttt ttggccttct cattggcctg ctccatcgtc atacaggtat
     9301 cggatgtggt ggcgttacac tcaaattccg tatttgttcc tgcgcagccg gaaagtaata
     9361 aggttcccag cagaggaata aataaagaaa tctgtttcat tgcccttctc cttttttatt
     9421 actccccaca acagcagaaa ttaactctgc tgtcagattt gttttccctg cacagggatg
     9481 tccggagacc actgtatttc ccggagaaga aaataagctc atcgacattc ccttcttcat
     9541 cacaccgaca gcagccatat taaatttgtg cggcaatgat tcagaactga acaccttcgg
     9601 agcaggaaga actatttttg tggtgcagtt actgagaaca gcatgcataa aatcatctga
     9661 tacgttttta tccggcacaa cgcgggctgt aataacatta atattacgca tgttcatttt
     9721 tatctccgtt gcttctgttt gttcggtcat cagggaaata aacatggcat ttcctgattt
     9781 ttcccggcag ggaagcgtct tccgtataaa cgtccttcag atattcatct gcggttacca
     9841 cccggataca tttcattttt ccgggcagca ccggatattt cccggtgatc gctgccagcg
     9901 agacatacat cagtgccagt gttgcccata atgcggtgac cagttgcatc agtgaccggt
     9961 cagtctccct gacataatct gcagcatcag ccgcgcggta atggcagata tcctgtattt
    10021 ctgatctgcc tgaagaatca gaggaagaaa accagcattc actgaccggg aactgtccgg
    10081 caggtgctgt ttgtcttacg aagttaatct ccttaaccag taatttcccc ggcgtggtgt
    10141 tgtccttcca taaaacaaac agagccatca gggccattgt ggccagtggc agaatgacgg
    10201 tgtacttcag acaccagaac agtcctcgca gaacccgggc gacaacatac cgggcggcat
    10261 ggcacgcctg acgggacgac atattatttg ccatcgttgc ctcctccctg gctgccggac
    10321 ataaactgct gcggattcag gttgcccagc tgcttcagca tctggtcagt attaaagccg
    10381 ttaagatgac tttcagctga tggcggaaca ggaaccggac tttccggatt atcttcttcc
    10441 gctctgttct gcgtgcgctc cagcgccatc tcttccacgg ttttcagctg gaagccgtcc
    10501 tggaacacca cggtcacttc gttgcccgca ccaatcggta tcaccgggtg atactgttcg
    10561 gcacgtttga tgtagtagtc actgagcgtc tgtgcggctt tcgatgtgcc gccaccgata
    10621 cccattttca ggacatcacc agccccgtaa gcggctgtgg cacccagccc gacagccggc
    10681 tgggaggcgc gctccattcc ctgaccgata ccgtcaacaa atcccgcgcc ccatgcccag
    10741 ccgaggattt tgccgttacg catcaccact tcgcccttga taccgttttt accccggaag
    10801 ctgacatgcc ccttaatcgg catatcaata gttttgccgt ccttcaggca actgatattg
    10861 cgggtgcgta caatggcacg ctcactggac acatcccccc aggcttccag ccccacaaaa
    10921 catcccgttg cgtcatacgt cttgctgttc ggcatttcca ccaggccggt gatacgcaac
    10981 tgcatcggca ccgtggattc attaccggtg actgaggcat tggcatccgc cccttcaatc
    11041 agcatggctt tcgcaaaact tcctgacgga atgtacggca gcgatggtcc ctgttttccc
    11101 tcattacggg taaacacctt acgctgtatc cggttaggca ccggcacaga ctggtacgtc
    11161 acctgtggtg gtggcgtgac accattcccg ggataaaacg ccgtcggcgg tggaacagca
    11221 acgctgccct gcggcgggaa ggatacgggg gtgtttcctg gctgtggttc gccttccggc
    11281 ccgggcggtg aggcaggcat ctgaggtaca ggctcaccgg tcgccgtgac gggattagca
    11341 cccagggctt ttacctgctc tgccagggcg gcattgtcct gtcccagctt ttcaatacga
    11401 cgctggtcat caccgcgctg tttgttcagg acatccagtt cacgacggat ttcctcatac
    11461 tgcttctgca tctgcgctgc cgtcacctgc atctctgtgg tggcatgctg acgcaccttg
    11521 tcatcaaagg tcgtatccac cacacctgtc atatccggca caggctcctg ttcagccacc
    11581 gcctcaccgt taccggacat gtccacatca gacagataca gtaccccacc aatcgcggag
    11641 gctgcaccga caaccacaat ccccagccac aggtactgct tgcgtttcac aatggtattg
    11701 atactggcca tcagttgccc tccccgtcac ggatgacata cacatccatg cgcgcacctg
    11761 ccagtaactg gctggcaggc tggctgaaca tcacggcacg ggttcccggc tgccagaagt
    11821 cactttcccg gatattcagg gcagacagcg ttttattttc cacggcaaag cggaccatct
    11881 tcaggtgatt ccccgtccat acggcatctg ccactgaaga cagccccgcc ggggcctgca
    11941 gggtttcctt tgtcacagga acctgatacc agcctgcagg taattttccg ccacggacag
    12001 cctggctgat ggtcacaagc agggattcgt aaggcgtgga cgtttcccac gctccggctt
    12061 cttctccggt tccgcgcagg tcactgacca gctgaatggt acgccccgcg ccttcacggg
    12121 gaacagcctg aatggaaagg ttcagaccac gctctgtttc cagaatgaac gtaaagggct
    12181 ttttgttgac ggtggccacc accacaccgc cggaggcggt ctgctcatta ttggtcaggg
    12241 caccatccag actgttcacg gcgataatcc ggtcaccggg aacggtaaac agattcgggc
    12301 tggtattgct gatggcaata ctggcctgac caccgttcac cattggcacc acagtggggg
    12361 ccagcgtacc gtttgcggcc atcacgctgc aggaaaaaaa caggccgccg aatattattg
    12421 ccgtattatt ttttctcatc gtctgtttcc ccgaaattat ccagccaggt caccccgttt
    12481 tcccgcttca gaataaggat gtaatgtttg atatcagtaa aaggttcgga gttaccaatc
    12541 catgttttcc gaattccacg gatttccaca cgaccatact gaggccagac acggacactg
    12601 gtctggaaaa aagcagaatt cacgttatct tttttaatga gtttcgcttc ttcagccaga
    12661 ataactttca tctggttctg tgcacccggg cggatatatt gcagaagcgc ctgatgtgaa
    12721 gcatcgacga tttccggtga gacattcaga cggagggcaa taaatgacag cgccatctgc
    12781 tgcaaataag aggcatcggc actgttctgt gacacggcaa agggcacatt aaatcccatt
    12841 ggggtcacgg cagtgcgttg ctcattctga agacggtagt tattcacccc ctgaatgagg
    12901 ttaacagaga ggctgagaac aataagcact gacataaata taaaggcgat ggccattaca
    12961 cgactggtac ttaaacgggc accgtgttcc atataacaat cctggtatca gttctattta
    13021 atccactgcc ggaaacacga atcgggaaca ttatgaaaaa taccgcgcag cagggctgtt
    13081 ggcatatacc agtaaatcag gtcacgtaac caggaactgc cccgcccttt tttcagtttt
    13141 ttaatcccga aataaaccag aaccgctgca ccaataccga acagatattt cgatgttgtg
    13201 ataccccagc caacacagat tgctgcgggg atcagttcat ccagtggcag gccaaaccag
    13261 cggctctggt tggtcagtgt ttccgggaaa cgatatttct taagtttatt ctcgtctccc
    13321 gacatcgttt tatttcctgt cagaggccaa cgacgctcat acccacagca ataaatacag
    13381 agatgatggc aaagccgacc aggaacttga cgtttttggt catcatgtac atgaccgcac
    13441 cgaccagcac ctcagccaga acaacccatt taacgatact ggagtcctta ccgaaggtcg
    13501 ccttaaccgt atcgttacct ttagccatca ggtcgccttt accagcagcc atagctaact
    13561 cagggaacaa catcatcaga acagcaaccg ggatcactgc acgaaccagg cgaagcatat
    13621 tcagacgagt gaatttagaa aaaaacgatt tattttcaac gggcgcagaa gcaccctgaa
    13681 cacttaaaac agtattcata tgattcctct gattaatatt gacagatgaa aactaaagtt
    13741 ctttaaatgt tgactcgttc tcttcgatca cctccctgaa tatttcttca ttataaaaat
    13801 ctggataacg ttgcaaatga tcctgcaacc tcaccaatgc ttcgtttgtt ttcgttcttc
    13861 cactacgctc tctggctcca agtaataatt tattcgtttc ctcatcaagg gtaagtaaaa
    13921 cttttctact tggcgcagat cttaaagctc ctttaatcac cctccctccc ataaataaaa
    13981 gagatttagt caaaatattc acataagacg cacacaaaaa taaacaacaa aatagtaaat
    14041 tacgttaaca aaaaccggca cttttagtgc cgggaatgct tacatttctt tacagtgaag
    14101 gctgttaata ttactagcga agtattgata tagataaaat aacacatcag agattatagc
    14161 atatcgcaac cactcatccc tgtctgagaa accatagtgt tttttaaaat tcataaatat
    14221 ttttttagat tggtcaacag agatatccat tctctgagaa gaatactcat gactataacc
    14281 caaaagatac ggtattatct tattaaagcg atatttgtca ggttcacaac taaactcctt
    14341 gccctttata aaaaagccac taactacgta tctttcaaag aatctccaga caaacaaatt
    14401 caagttattc acttccgtac tttcaaccac cacatcccat gatacatcat tcaatattac
    14461 accattaata atactcactc cattcgagga cgagaaagca tcaacttcag cagtatgaaa
    14521 taaaatttgc gtatcaatat caataccaga aaaccatatt tcagaatcac tattctgaca
    14581 ctttattaag tgtaaaaaaa cagagtttga gtctatgaat tttcctgact ctgtgcggac
    14641 acagactgga tattttgcgg ataaaataat ttccggcagg cctgattgac gtatgagtgc
    14701 tctttctcta gtatccatcg cacacatagg aacctcctca aaggattcta tggacagtcg
    14761 atgcagggag ggacaagctc cctgcatcgg cgattttcaa gttcaaattt taacgtggca
    14821 ttaaccacgt aattaatata ctaattattc tgattattgt catcagtttg aaccattaaa
    14881 aagtctgata tttgaacaaa gtcaaaattt atttcccttt attcatcatc attttttgga
    14941 aagaaccttt ccatctcagc cgacaccttc tctctgatat cttcaaccat attggcatat
    15001 tcaaactttg gatttcctga gacatgagga ctgagagact caatacccaa aattttcgct
    15061 actgatgact gtgtttttac aacgcattca agaagtagtt tattaaactc ggcctgatta
    15121 aatgcagact ctttgcgctc catctgagcc tcatatacac gaagacccag ttcaagaagc
    15181 attgaagccg ttgctgaaaa actgacatct ttttctctcg ccccctcctg tcgacgtttt
    15241 tcaataatcg cgtttatttt ttcataagca tcattgctga tatacagatt caccttagcc
    15301 attagatatt acctttttta aacataaaac aaagttaatt tcgaaacact agaattcata
    15361 gtgcaaacag ggacgcaccg ctagcagcgc ccctgacggt atcctataaa aaaacacacc
    15421 gcgccgctag cagcacccct aatataaaat aatgtttttt ataaaaatag tcagtaccac
    15481 ccctacaaag cggtgtcggc gcgttgctgt agctgcgtta acgacgctgc tttaaaaaaa
    15541 tcagatttaa acaatataaa tccacaaata caactcaatg atattaaaga taaatcagca
    15601 aaaacttgtt tttgcgtagt gtgtggtgct tttggtggtg agaaccacca acctgttgag
    15661 cctttttgtg gagtgggtta aattatttac ggataaagtc accagaggtg gaaaaatgaa
    15721 aaaatggatg ttagcaatct gcctgatgtt tataaatggg atctgcgaag ccgccgattg
    15781 ctttgatctt gcaggtcggg attacaaaat agacccggat ttactgagag cgatatcatg
    15841 gaaagaatcc cgttaccggg ttaatgccat cggtattaat ccggtaacgg gatatggcag
    15901 cggactgatg caggtagatt cccagcattt taacgaactg gcccgctatg gaattaagcc
    15961 ggaacatctg acaacagatc cctgtatgaa catttatacc ggtgcttatt atctggcaat
    16021 agcctttaaa aaatggggcg tctcctggga ggccgttggt gcatacaatg ccggattcag
    16081 gaagaccgaa cgccagaacc agagacgtct tgcctacgca tcagaggttt accggattta
    16141 tacctggata aagagcagta aaggcatccg ggttccgacc acgaagaaat cactttccca
    16201 aattaacagt gtgcagaaaa attaatattg tatacattgt caaataccgg ttatgccgcg
    16261 acagcggcat ggaaggccac acacaaaaat atcatatgag tgataatgct gactggccga
    16321 agccccgcaa agcgggatcc catcttatat atatccggta actgcgcagc ctcctgccag
    16381 accatttttc gcgggcacaa tatttacaga ctaccgaaaa gccagttgat gaaattgagg
    16441 catatcagac gacacagcaa tgccgcagga tgataatata agtaaagccc cgaaaaattt
    16501 ttcggggctt tacttatatt atcatgactt ccactccctg aactgttcgg caatcatcca
    16561 taatgctttg ttgattcgga tatcaccgtc aatgcctgta atggctcttg ttctggtatt
    16621 tttaccggag gcacttcgcc ccgataatcc accttttatc atattctcct gaacccgctg
    16681 ccaggttgtc cagagatcgt tctgtttatc ctcccaacgg cggggagtaa ttatttgttc
    16741 tggcgtcact ggcgttttat tttcatcttc gtacctggcc atcaatgcag ctctgccaaa
    16801 taaatgttgt tcgtcactgt taagatgtat ctctttcatt gtctccatat tttcagtgac
    16861 tttatcaaag acaccgagca cttcatacgc tccctcaata acctgcccga caatatcacc
    16921 tttatgtgga acgcggattt cgccaaaatt attcccacac accaggccat ttgtgcagac
    16981 aaaacgaaaa attcctggga tcatctgata actggatgaa ccatcatgtg aattaagcag
    17041 gataatttca ggaacttctt tcccgttaat atgcccttcc ctgcgaagac gtaacatatg
    17101 tttactgtat tcgcggcgtc ccaaatcacg aacccgactc tgacaggcaa agaatggctg
    17161 gaaaccttca tcacgtaact tattgatgat attgatggtt gggatatatg tataacgctc
    17221 actccgggat tcatgtttat caccagaaaa gacagatgga acaaactgca ttaattcgtc
    17281 atccgttaac gggcgctcac ggcgaataga attatatcga ccaaaacggg aagctaatcg
    17341 cataacagta actcctttat gaaggcataa aattaataaa agttttttcg cttaatatca
    17401 tcccccctcg gttatcgtta acaaaaagac aacaattccg aggggaagga ccatcaaatc
    17461 aggtcagttc gggactgacc aacctgccga gtgaaaagca gtaacagcgc gatcaactca
    17521 tcagcgtttt ttctgacaag aggagtatga ttctccaggc tatccagtaa gtcatgcagc
    17581 tcattattga cctggtggag caccttaaag agtgcaccag aatcccggca gctctgatat
    17641 gcaagatttg cctgaacagc acgattctgc atgatcccga tttgtgcgat tgcgtctttc
    17701 tgctgatcgc ctgttggctg ataagtggtg ttaaaagaaa atgcgctcat aattcctcct
    17761 gtatctgatg ttttcagccc ccatcatctg ttgagtttcg ggagccgcgg ttttgacgtt
    17821 ttgcggggac ctgccttttg ttccctccgg tattcaccat cacc
//
LOCUS       DAZSNO010000073        17815 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZSNO010000073 DAZSNO010000000
VERSION     DAZSNO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17815
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.073"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..509
                     /locus_tag="ACHWN2_002361"
     CDS             <1..509
                     /locus_tag="ACHWN2_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR8931873.1"
                     /translation="AAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSV
                     SINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDK
                     AIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSS
                     LVMRVAQD"
     gene            590..1264
                     /locus_tag="ACHWN2_002362"
     CDS             590..1264
                     /locus_tag="ACHWN2_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR8931874.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1280..3760
                     /locus_tag="ACHWN2_002363"
     CDS             1280..3760
                     /locus_tag="ACHWN2_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR8931875.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3776..4809
                     /locus_tag="ACHWN2_002364"
                     /pseudo
     CDS             3776..4809
                     /locus_tag="ACHWN2_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4891..5229)
                     /gene="rcnB"
                     /locus_tag="ACHWN2_002365"
     CDS             complement(4891..5229)
                     /gene="rcnB"
                     /locus_tag="ACHWN2_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR8931876.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5448..6278)
                     /gene="rcnA"
                     /locus_tag="ACHWN2_002366"
                     /pseudo
     CDS             complement(5448..6278)
                     /gene="rcnA"
                     /locus_tag="ACHWN2_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6400..6672
                     /gene="rcnR"
                     /locus_tag="ACHWN2_002367"
     CDS             6400..6672
                     /gene="rcnR"
                     /locus_tag="ACHWN2_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR8931877.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6764..6860
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6895..7683
                     /gene="thiM"
                     /locus_tag="ACHWN2_002368"
     CDS             6895..7683
                     /gene="thiM"
                     /locus_tag="ACHWN2_002368"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR8931878.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7680..8480
                     /gene="thiD"
                     /locus_tag="ACHWN2_002369"
     CDS             7680..8480
                     /gene="thiD"
                     /locus_tag="ACHWN2_002369"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR8931879.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8545..9362
                     /locus_tag="ACHWN2_002370"
                     /pseudo
     CDS             8545..9362
                     /locus_tag="ACHWN2_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            9414..10160
                     /locus_tag="ACHWN2_002371"
     CDS             9414..10160
                     /locus_tag="ACHWN2_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR8931880.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10134..11099)
                     /locus_tag="ACHWN2_002372"
     CDS             complement(10134..11099)
                     /locus_tag="ACHWN2_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR8931881.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11096..12099)
                     /locus_tag="ACHWN2_002373"
                     /pseudo
     CDS             complement(11096..12099)
                     /locus_tag="ACHWN2_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12139..13191
                     /gene="fbaB"
                     /locus_tag="ACHWN2_002374"
     CDS             12139..13191
                     /gene="fbaB"
                     /locus_tag="ACHWN2_002374"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR8931882.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13499..14353
                     /gene="gatY"
                     /locus_tag="ACHWN2_002375"
     CDS             13499..14353
                     /gene="gatY"
                     /locus_tag="ACHWN2_002375"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR8931883.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14382..15644
                     /gene="gatZ"
                     /locus_tag="ACHWN2_002376"
     CDS             14382..15644
                     /gene="gatZ"
                     /locus_tag="ACHWN2_002376"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFR8931884.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGHALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15654..16106
                     /gene="gatA"
                     /locus_tag="ACHWN2_002377"
     CDS             15654..16106
                     /gene="gatA"
                     /locus_tag="ACHWN2_002377"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR8931885.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16137..16421
                     /gene="gatB"
                     /locus_tag="ACHWN2_002378"
     CDS             16137..16421
                     /gene="gatB"
                     /locus_tag="ACHWN2_002378"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR8931886.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16425..17780
                     /locus_tag="ACHWN2_002379"
     CDS             16425..17780
                     /locus_tag="ACHWN2_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR8931887.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4630 a         4085 c         4547 g         4553 t
ORIGIN      
        1 ttgctgccgc tgtcttttct tcttttttta tgagcgctgg agtatttgct gcagacgttg
       61 ataccggaac attaaccatt aaaggaaata ttgcagaatc tccgtgtaaa ttcgaagcgg
      121 gtggtgattc agtaagtatt aatatgccga ctgtaccaac cactgtcttt gaaggtaaag
      181 ctaaatattc tacctatgat gatgcagtcg gtgtaaccag cagcatgtta aaaattagct
      241 gcccgaaaga agttgctggt gtaaaactct cgttgatcac aaacgacaaa ataaccggta
      301 acgataaggc gatagccagt agcaacgata ccgtaggtga taacagcgat gtcctagatg
      361 tttctgcacc ttttaacatt gagagttata aaacagcgga aggtcaatat gctattccgt
      421 ttaaagcaaa atacctgaaa ctgacagata actcagtgca atcaggtgat gtgttatctt
      481 ctctggttat gcgtgtggcg caggattaat aaataaaatt gcaagtttct ccaggtaata
      541 aacatggccg ctatccccat ggcggctttt taatttaaga ggcattaaga tgaaaggatt
      601 attatcttta ctcatttttt ctatggtcct tcctgcacat gccggaattg ttatctacgg
      661 gacgcgcatt atttacccgg cagaaaataa agaagttatg gtgcagttga tgaaccaggg
      721 aaatcgttct tcgctgctgc aggcgtggat tgatgatggc gatacgtcgt taccaccaga
      781 aaaaattcag gttcctttca tgttaacgcc accagtggca aaaatagggg caaattccgg
      841 gcagcaagta aaaatcaaaa ttatgccgaa taaactgccc actaataaag aaagcatttt
      901 ttatctgaat gttctgtata ttccaccaaa tagtccagag caagaaggca agaatgcact
      961 gaagtttgcg atgcaaaaca gaattaagtt gttttaccgg ccagcgggta ttgctccggt
     1021 aaataaagcg acctttaaaa agttactggt aaatcgcagt ggcaatggat tggtgataaa
     1081 aaatgactca gctaattggg tgacgatttc ggatgtcaaa gctaataatg ttaaagtcaa
     1141 ttatgaaact attatgattg cccccttaga aagtcagagt gttaatatca aaagtaataa
     1201 tgcaaataac tggtatctga ccattatcaa tgaccacggc aactatatta gtgacaaaat
     1261 ttaattgcag gagctgccta tgttgagaat gaccccactt gcatcagcaa tcgtagcgtt
     1321 attgctcggc attgaagctt atgcagctga agaaaccttt gatacccatt ttatgatagg
     1381 tggaatgaaa gaccagcagg ttgcaaatat tcgtcttgat gataatcaac ccttaccggg
     1441 gcagtatgac atcgatattt atgtcaataa gcaatggcgc gggaaatatg agattattgt
     1501 taaagacaac ccgcaagaaa catgtttatc aagagaagtt atcaagcggt taggcattaa
     1561 tagcgataac ttcgccagcg gtaagcaatg tttaacattt gagcaacttg ttcagggtgg
     1621 gagctatagc tgggatatcg gggtttttcg tctcgatttc agtgtcccgc aggcttgggt
     1681 ggaagaactg gaaagtggct atgttccacc ggaaaactgg gagcggggta ttaatgcgtt
     1741 ttatacttct tattatgtga gtcagtatta cagcgactat aaagcgtcgg gtaacaacaa
     1801 gagtacatat gtacgtttta acagcgggtt aaatttactg gagtggcaac tgcattctga
     1861 tgccagtttc agtaaaacaa ataacaatcc aggggtgtgg aaaagcaata ccctgtatct
     1921 ggaacgtgga tttgcccaat ttctcggcac gcttcgcgtg ggtgatatgt acacatcaag
     1981 cgatattttt gattctgttc gcttcagcgg tgtgcggttg tttcgtgata tgcagatgtt
     2041 gcctaactcg aaacaaaatt ttacgccacg ggtgcagggg attgctcaga gtaacgcgct
     2101 ggtaactatt gaacagaatg gttttgtggt ttatcagaaa gaggttcctc ctggcccgtt
     2161 tgcgattaca gatttgcagt tggccggtgg tggagcagat cttgatgtta gcgtgaaaga
     2221 ggcggacggc tcggtaacca cctatctggt gccttatgca gcggtgccaa atatgctgca
     2281 acccggcgtg tcgaaatatg attttgcggc gggccgtagc catattgaag gggcgagcaa
     2341 gcaaagtgat tttgtccagg caggttatca gtatggtttt aataacttat tgacgctgta
     2401 tggcggcacg atggttgcta ataattacta tgcgtttacc ctcggaacgg gttggaacac
     2461 gcgcattggc gcaatttccg tcgatgccac gaagtcgcat agtaaacaag acaacggcga
     2521 tgtgtttgac gggcaaagtt atcaaattgc ctacaacaaa tttgtgagcc aaacgtcgac
     2581 gcgttttggt ctggcggcct ggcgttattc gtcgcgtgat taccggacat ttaacgatca
     2641 cgtttgggca aacaataaag ataattatcg ccgtgatgaa aacgatatct atgacattgc
     2701 cgattattac cagaacgatt ttggccgtaa aaatagcttt tctgccaata tgagccagtc
     2761 attgccagaa ggctggggtt ctgtgtcctt aagtacgtta tggcgagatt actgggggcg
     2821 tagcggcagc agtaaggatt atcagttgag ttattccaat aactggcggc ggataagcta
     2881 taccctcgcg gcaagccagg cgtatggcga gaatcatcat gaagagaaac gttttaatat
     2941 ttttatatca attccctgtg attggggtga tgacgttacg acgcctcgtc ggcaaatata
     3001 tatgtctaac tcaacgacgt ttgatgatca gggttttgcc tcaaataata cgggattatc
     3061 aggaacagta gggagtcggg atcagttcaa ttatggtgtc aacctgagtc atcaacatca
     3121 gggaaatgaa acgacagctg gggcgaattt gacctggaac gcgccggttg cgacagtgaa
     3181 tggcagttat agtcagtcga gtacttatcg acagactgga gccagtgttt cagggggcat
     3241 tgtcgcctgg tcgggtggcg ttaatctggc gaaccgtctt tccgaaacgt ttgctgtgat
     3301 gaatgcgcca ggaattaaag atgcttatgt caatgggcaa aaatatcgca caacaaaccg
     3361 taatggagtg gtggtatacg acggaatgac accttatcgg gaaaatcacc tgatgctgga
     3421 tgtgtcgcaa agcgatagcg aagcagaatt acgtggcaac cggaaaattg ccgcccctta
     3481 tcgcggcgcg gttgtactgg ttaattttga taccgatcag cgcaagccct ggtttataaa
     3541 agcgttaaga gcggatggtc aaccattaac gtttggttat gaagtcaatg atatccatgg
     3601 tcataatatt ggcgttgtcg gccagggaag ccagttattt attcgcacca atgaaatacc
     3661 gccatcggtt aatgtagcaa ttgataagca acaaggactt tcatgcacaa tcaccttcgg
     3721 taaagagatt gatgaaagta gaaattatat ttgccagtaa tagtcaggtg gttctatgga
     3781 aattcgcata atgctattta tattaatgat gatggttatg cctgtgagct atgcggcatg
     3841 ttatagtgag ttatctgttc agcacaattt ggttgttcag gggaattttg cacttactca
     3901 aacacaaata gcgacatatg agcataattt taatgattcg tcatgcgtga gtacaaatac
     3961 tatcacccct atgagcccat cggatattat tgttggactt tataacgata ccattaaatt
     4021 aaatttacat tttgaatgga ccaataaaaa caacatcacg ttgtcaaata atcagagcag
     4081 tttcaccagt ggttattcgg taacggtgac acctgcggcc agtaatgcaa aagtgaatat
     4141 ttctgcgggg ggcggtggtt cagtgatgat taatggtgtt gcgacattat ccagtgcttc
     4201 atcatcgaca cgcgggagtg ccgcagtaca atttctactg tgtttattag gtggcaagtc
     4261 atggggtgca tgtgtaaata gctacagaaa tgcattggca caaaatgcag gtgtctattc
     4321 cttcaatctg acattgtcat acaacccgat aaccacaacc tgcaaacagg acgatttatt
     4381 aattacttta gacagtattc ccgttttaca attaccagcc acaggtaaca aagcaacaat
     4441 aaatagtaaa aaaagggata ttattctgcg ttgtaaaaat ttattaggtc aacaaaatca
     4501 aacatcacgg aaaatgcagg tgtatttatc aagttctgac ttgttaacca acagcaacac
     4561 aatactgaaa ggtgcggaag ataatgcgta ggatttattc ttgaaagtaa tggttcgcca
     4621 gtcacacttt taaatatcac taacagcagt aaaggataca caaatttaaa agaaattgcg
     4681 gcaaagtcaa aacttacaga tacaacggtt tcaattccga taacagccag ttactacgtc
     4741 tacgatacaa acaaagttaa atctggcgca ctggaggcaa ccgcattaat caacgtgaaa
     4801 tacgactaat aacaagcaaa acgagcaagt ggctaataat aaaaaataac aaggtgtctg
     4861 catttccccc tcatgatgag ggggcttttt ttagcgatga taaaaaatct caccgtcgta
     4921 ggctttaatg attttaccgt cggtgtcgct gatcaacacg tacgcgccac ccatataggt
     4981 ccagtgcgtc ccggcatcag gcgcgggcag gttacgtaac tgccactgct taatgttgta
     5041 ctcatcggtg cggtacatct ctgggacggt atcacctggt ttgaaatgct taaagtcagc
     5101 aatgaaacta cttaactcat acttagaaat tcccgaggta ttggtcgaac ccgcggtggc
     5161 tggtgcggcc caggcggcac tggtaaccag caaaagcaca cccagcaaca tcttattttt
     5221 aatggtcata aactctccac tttggggatc tgtaatatca gtcgcatatc tttcctgtca
     5281 tacaatataa ctgacaagcg atattttgtt agtgatccca tgatttcgta aaaagtgtaa
     5341 atgaaaggat gccctaaaag catccttagc gagcagagag tcagcggaaa tagctgattt
     5401 aagaatcatc ctgggctgaa gataacatgc aagcctaaag gattgagtta tcgcattatg
     5461 cccatgaagc catgtacgcc catatacaca ccgactaagc caatcaacag actggaaaaa
     5521 tagggggcgc gtttagcgag agtgttaaat ccgctccagc gttttgcgac ctgctgaacg
     5581 ctgattgctg cgccaacgcc tacggtgaca agcgttaacg ccaggccaat gctgaaactg
     5641 acgaccagtg ttgcgcccag tgtcagggct ttcaactgaa tgcatatcaa cagcacggta
     5701 attgctgccg ggcaggggat aaagccaccg gttaagccaa ataacaaaat ttgccagttg
     5761 atgacctctc taccatcaaa gcgtcgttta atgtcattgg catgggctcg tgcatgggca
     5821 tcctgatact cgccatgttc gtgatggtga tgatgtcctt ggtcgtggtg atgttcgtga
     5881 tcgtgatgat ggtgatgatg ctcataatca tgcccgtgca tattctccag ccagttgcgt
     5941 tcgccgcgct aggtacgcca gaacatccag aacgcggtgc caataatgat cactgcggaa
     6001 atcagctgga gccacggttc tgctgattga gcagtaaagc gcttactgat caccatcccg
     6061 ccaaaggcaa tcaaccagac caccgcggta tgcgaaatgg ttgctgccag tccgagcatc
     6121 accgcctgtt taatggtgcc tttgatggcg atgataaacg ccgccatcat cgtttttgag
     6181 tgccctggtt ccaggccatg aagcgcacca agtaagatgg cgctggggat gaagaaccag
     6241 gcgtttcctt gctgaagaag agttgtaaat tcggtcatga taataattct tagtattaat
     6301 tcggcaatct gattctactc ccccccagta cctgatacta cccccccagt agattaatag
     6361 tgctatgatt tttcatattc ttgttaacca ggtgttgcca tgtctcatac aatccgtgat
     6421 aaacagaaac tgaaagcgcg tgccagtaag attcagggcc aggtcgtggc gctcaagaaa
     6481 atgctcgacg agccgcacga atgcgctgca gttttacaac agattgctgc tatccgtgga
     6541 gcggtaaacg gtctgatgcg ggaagtgatt aaaggtcatc tgacggaaca catcgttcac
     6601 cagggggatg agctaaaacg tgaagaagat ctggatgtcg ttctgaaggt gctggattca
     6661 tatatcaaat aatttattaa cgcgattgta aaactgccgt ttttcctcgt ttacaacggg
     6721 ggcgctggac attaccatcc tcctctgcga tttatcatcg caaccaaacg actcggggtg
     6781 cccttctgcg tgaaggctga gaaatacccg tatcacctga tctggataat gccagcgtag
     6841 ggaagtcacg gaccaccagg tcattgcttc ttcacgttat ggcaggagca aactatgcaa
     6901 gtcgacctgc tgagttcagc gcaatctgcg cacgcgttac acctttttca ccaacattcc
     6961 cctcttgtgc actgcatgac caatgatgtg gtgcaaacct ttaccgccaa taccttgctg
     7021 gcgctcggtg catcgccagc gatggttatc gaaaccgaag aggccagtca gtttgcggct
     7081 atcgccagtg ccttgttgat taacgttggc acactgacgc agccacgcgc acaggcgatg
     7141 cgtgctgccg ttgagcaagc aaaaagctct caaacacgct ggacgcttga tccagtagcg
     7201 gttggtgcgc tcgattatcg ccgccatttt tgtcatgaac ttttatcttt taaaccggca
     7261 gcgatacgtg gtaatgcttc ggaaatcatg gcattagctg gcattgctaa tggcggacgg
     7321 ggagtggata ccactgacgc cgcagctaac gcgatacccg ctgcacaaac actggcacgg
     7381 gaaactggcg caatcgtcgt ggtcactggc gaggtggatt atgttaccga tggacatcgt
     7441 atcattggta ttcacggtgg cgatccgtta atgaccaaag tggtaggaac tggctgtgca
     7501 ttatcggcgg ttgtcgctgc ctgctgtgcg ttaccaggcg atacgctgga aaatgtcgca
     7561 tctgcctgtc actggatgaa acaagccgga gaacgcgcag tcgccagaag cgaggggcca
     7621 ggcagttttg ttccacattt ccttgatgcg ctctggcaat tgacgcagga ggtgcaggca
     7681 tgaaacgaat taacgctctg acgattgccg gtactgatcc gagtggtggt gcggggattc
     7741 aggccgatct taaaaccttc tcggcacttg gcgcttatgg ttgctcagtt attactgcac
     7801 tggtggcgca aaatacccgt ggcgtacagt cggtgtatcg cattgagcct gattttgtcg
     7861 ccgcccagct cgattcggtg ttcagcgatg tgcgaatcga taccactaaa atcggtatgt
     7921 tggcggaaac cgatattgtt gaagcggtgg cagaacggtt gcaacgttat cagatccaaa
     7981 acgtggtact cgacaccgtt atgctggcaa aaagcggcga cccgctgctt tcaccttcgg
     8041 cggttgctac gctgcgcagt cgattattgc cacaggtttc attaataacg ccaaacttgc
     8101 ccgaagctgc cgccttgctc gacgcgccac acgcgcgcac cgaacaggaa atgctggaac
     8161 aagggcgatc gctgttggcg atgggctgtg gcgcagtgct aatgaaaggt ggtcatctgg
     8221 atgatgagca aagcccggac tggctgttta cccgcgaggg tgaacaacgg tttaccgcac
     8281 cgcgcattat gaccaaaaac acccacggca ctggttgtac actctctgcg gcgttggctg
     8341 cactacgccc gcgccataca aactgggctg acaccgtaca ggaggcaaaa atctggcttt
     8401 catcggcgtt agcccaggcc gacacgctgg aagttggtca cggtattggt ccggttcacc
     8461 acttccacgc ctggtggtga gcaccgtact atagctgctt tggttattgt cagcctttgg
     8521 cactgacatt cagggatgaa accaatgcag ctaagaatta ccagtcgaaa aaaactcacc
     8581 gctctattat gcgctcttgg gctaatttcc atcgttgcaa tttatccgcg tcagacggtg
     8641 aattttttct attcgacagc agttcagatt acagactaca tccacttcta cggttatcgc
     8701 ccggttaaat ctttcgctat tcgcattcct gccagttaca ccattcacgg attagatgtt
     8761 tcgcgctggc aggagcgtat cgactggcag cgggtggcaa aaatgcgcga caacgatatc
     8821 cgcttacagt ttgcttttat taaggctacg gaaggcgaaa agctggtgga cccctatttt
     8881 tcgcgtaact ggcaattaag ccgcgaaaat ggcctgctgc gcggggcgta tcattatttc
     8941 tccccgtcgg tatccgcttc agttcaggcg cgattatttc tgcaaacggt ggatttctca
     9001 caaggcgatt tccctgccgt gctggacgta gaagaacggg gaaaattatc ggcaaaagaa
     9061 ttacgcaagc gggtaagtca gtggctaaaa atggtcgaaa aacgcacggg aaaaaagccg
     9121 attatttact caggagccgt tttttatcac accaatctgg cgggctattt caatgaatat
     9181 ccgtggtggg tggctcacta ttatcaacgt cgtccggaca atgacggcat ggcctggcgc
     9241 ttctggcagc attccgaccg tggacaggta gatggcatca atggtccggt ggattttaat
     9301 gtgtttaatg gcacggggat gagttgcagg cattcgttga tgggattaaa gaaacgcctt
     9361 aaataagaag cagtgatata ctcccaccag actccctggc aggaaaaaga caaatggaac
     9421 aagcgcatac ccagctgatt gcccaactga acgagcgtat tttagcggcg gataacacgc
     9481 cgctttatat taagtttgcc gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc
     9541 atggcaatat tttgcccggt gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta
     9601 cggtgcgcaa ggcgatgcag gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt
     9661 acggcacgca gatcaacaac atcttcgaat actcgttgaa agaagcgaga ggtttttctc
     9721 agcaggtggt attgcgcggg aaaaagcccg atacgctatg ggttaataag cgtgtcgtga
     9781 aatgtcccga agaagttgcg cagcagctgg cggtcgaagc gggaagtgat gtctttttgc
     9841 ttaagcgtat tcgctatgtc gatgaagaag cggtatcgat tgaggaatcg tgggttccgg
     9901 cgcatttaat tcatgatgtt gatgccatcg gaatttctct ttatgactat ttccgcagcc
     9961 agcacattta cccacaacgt acgcgttccc gcgttagcgc ccggatgccg gatgccgagt
    10021 ttcagtcaca tattcagtta gatagcaaaa taccggtgct ggtgatcaag caagtggcgc
    10081 ttgaccaaca gcaacggcct attgagtaca gcattagcca ctgtcgcagc gatctatacg
    10141 tttttgtgtg cgaggagtag ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg
    10201 acaacccacg acgccactgc attgcccagt aatacggcat ccgccagtgg cagaccagag
    10261 gccagcccgg caagtacgcc acctgcatga ctgtcgcccg ccccaatggt gtctacaact
    10321 tgcgttggaa atgccggaat gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct
    10381 ttatcgagac gaacgatcaa cggcgcggca aatttctcct gccattgctt gccaagtgtt
    10441 gttatctctg cggataaagc aaaacgttcg gcggcaatct cagcctcttg acgattgagt
    10501 gacactaaag gtcgacaggc catgatccgc gccagtaatg cgtccgggat atcgccaata
    10561 cgtgggcaaa aatcgataaa cggcgtcacg tcttgcagct tttccagcca ttccactaac
    10621 aattcgccgc agggcgaggc cagttgataa ccggaaaaat agagcaggtt gccaggcgca
    10681 acggttaatc gtgccaacca ttggcgattc cattgatttt ccacaccgct gaatgacata
    10741 aaagtgcgtt cgccatccgg ctcaaccagt gccagacacc agccgttatc gctttcggcg
    10801 ttatcgatca ggctgattaa cccctctttc gccatccggt tgcgaataat ctccgcccac
    10861 acgccctgac cgagcggcaa ggcattaccc gcttcgatgc cgaggcgctt taacgccacg
    10921 gcaatattca gtgcgcagcc gccaacgtta acgctctgct gtttcagttc gatatcgcac
    10981 ccacgccagg ggagggcata agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc
    11041 atcaccggct gacgcgtggt taattcaggc agcagtgtgt gtaatcgagc gccgctcata
    11101 ccgcctcccg ttgttgacga tatttcgcca gcgctgtggc atagcggttg aaatcaagct
    11161 gatttaccgc atccagctcc gcctttaacg caggatcgac agcgttaatg ccatgcaacg
    11221 cgccgcaaat cgccgtcgcc atagcaccta tggtgtctgt gtcgccgcca agattggcgc
    11281 acaggacggc gcagcggttt gggtcggttt gtgccagttc aactagcgca atggcgcacg
    11341 gaacggactc aatagtgctg gtgcctgcgc caacgacctg gtaaagctgt tcgctggcgg
    11401 attcggtgcc gtcggcatta cgcacaattt tcagcgcaat ctccagacgt gccgccagtg
    11461 aggcgctgaa cgtggtggtg cgtttttgct gtgcatgtcg cgcaattgaa ggcaatgaat
    11521 caacaatcgc tgaccagcgt tctccgtcaa tggcacgaga aatcgcccat gcgatgacta
    11581 ccgcgcctgc aaccgccaga tcggatttat gggtcgggct ggacgccagc gccacatcat
    11641 caataaagga atcaacatca cgcgctggaa gcaaacaacc taatggcgag acgcgcatcg
    11701 ctgcgccgtt ggttacgccg ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg
    11761 cgttaagcgc aatcttcgag gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca
    11821 acgcccagtc gagaatatta cgcccaatca gattcggatc gatcttgcct tcacattcca
    11881 gtaacgcatc cgccagacac aggccatcga ggtatcgtcg gtgaattcgg cgcggttaaa
    11941 ataacaggcc gcgttattct cctttggtcc aggcagaaaa cggtcaatcc agccaaagtg
    12001 tgctttaacg cggctgcgtg gccaaagctc ggagggcatc cccatcgcat cccctaacgc
    12061 ctgcccataa agagcaccga gaatacgttc tgttttcatt taacttcccc ttgaatcgga
    12121 atttacggga gcatagtaat gacagatatt gcgcagttgc ttggcaaaga cgccgacaac
    12181 cttttacagc accgttgtat gactattcct tctgaccagc tttatctccc cggacatgac
    12241 tacgtagacc gcgtgatgat tgacaataat cgcccgccag cggtgttacg taatatgcag
    12301 acgttgtaca acaccgggcg tctggctggc acaggatatc tttctattct gccggttgac
    12361 cagggcgttg agcactctgc cggagcttca tttgctgcta acccgctcta ctttgacccg
    12421 aaaaacattg ttgaactggc gatcgaagcg ggctgtaact gtgtggcatc aacttacggc
    12481 gtgttggtgt cggtatcgcg gcgctatgcg catcgcattc catttctcgt caaacttaat
    12541 cacaacgaga cgctaagtta cccgaatacc tacgatcaaa cgctgtatgc cagcgtggag
    12601 caggccttca acatgggcgc ggtggcggtt ggtgcgacta tctattttgg ctcggaagag
    12661 tcacgtcgcc agattgaaga aatttctgcg gcctttgaac gcgcgcacga gctgggcatg
    12721 gtgacagtgc tgtgggccta tttgcgaaac tccgccttta agaaagatgg cgttgattac
    12781 catgtttccg ccgacctgac tggtcaggcg aaccatctgg cggcaaccat aggtgcagat
    12841 atcgtcaaac aaaaaatggc ggaaaataac ggcggctata aagcgattaa ttacggttat
    12901 accgacgatc gcgtgtacag caagttaacc agcgaaaacc cgattgatct ggtgcgttat
    12961 cagttagcta actgctatat gggccgggct gggttgataa actccggcgg tgctgcaggc
    13021 ggtgaaactg acctcagcga tgcagtgcgt actgcggtta tcaacaaacg cgcaggcgga
    13081 atggggctga ttcttggacg taaggcgttc aagaaatcga tggctgacgg cgtgaaactg
    13141 attaatgccg tgcaggacgt ttatctcgat agcaaaatta ctattgcctg atacgcctca
    13201 tacgaacagc accaccccgt aggacggata agacgtttac actgcatccg gcaacctatg
    13261 cctgatgcga cgcggaagcg tcttatcatg cctacatagc actgccacgt atgtttacac
    13321 cgcatccggc ataaaaacac gcgcactttg ctacggcttc cctatcggga ggccgttttt
    13381 ttgcctttcg ctcctcgaac aattttcata ttgttgtttt tgcgatcatt atctcgacat
    13441 ttaaaagcaa ataatttcat tatattttga aatcgaaagc aaacgacaag atatgaaaat
    13501 gtacgtggta tcgacaaagc agatgctgaa caacgcacag cgcggcggtt atgcggttcc
    13561 ggcattcaat attcacaatc tcgaaacgat gcaagtggtg gtggaaaccg ctgccaacct
    13621 gcatgcgccg gtcatcatcg ccggaacgcc tggcacattt actcatgctg gtacagaaaa
    13681 tctgttggcg ctggtcagcg cgatggcgaa gcaatatcac catccactgg caattcatct
    13741 cgaccatcac acgaaatttg acgatatcgc tcagaaggtt cgttctggcg tgcgctcagt
    13801 catgattgac gcctcgcatt tgccttttgc gcaaaatatt tcacgggtca aagaggtggt
    13861 ggatttttgc catcgctttg atgtcagcgt cgaggcggag ctggggcaac ttggcggcca
    13921 ggaagatgat gtgcaagtca atgaagtcga tgcgttgtac accaaccccg cacaggcgcg
    13981 tgaatttgcc gaggcaaccg ggattgattc cctggcggtc gccatcggca cggctcacgg
    14041 tatgtatgcc agcgcaccgg tgcttgattt ttctcgactg gagaacattc gccagtgggt
    14101 gaacttaccg ctggtgctgc atggcgcgtc aggattatcg actaaggata ttcagcaaac
    14161 catcaaactg gggatatgca aaatcaacgt tgcaacggag ctgaaaaatg ccttcttgca
    14221 gtcgttaaaa aattacctga ccgagcaccc cgaagcgacc gatccccggg attatttgca
    14281 gtcggctaaa tccgcaatgc gcgatgtggt gagcaaagtg attgccgatt gtggctgcga
    14341 gggcagggca taacgcacct gccatttaac aaggaaaaaa catgaaaacg ttaattgccc
    14401 ggcataaagc tggtgaacat atcggcatat gttcagtctg ttctgcccat ccgttggtta
    14461 tcgaagcggc gctggcattt gatcgcaaca gcacgcgcaa agtgctgatt gaagcaacgt
    14521 caaaccaggt caatcaattt ggcggttata ccggaatgac accggcagac tttcgcgaat
    14581 ttgtttttac gattgccgat aaagttgggt ttgcacgcga acgcattatt ctcggcggcg
    14641 atcatctggg accaaactgc tggcagcaag aaaatgcgga tgcggcgatg gaaaaatccg
    14701 tcgagctggt aaaagcatat gttcgtgctg gcttcagtaa aattcatctt gatgcgtcaa
    14761 tgtcctgcgc gggggatacc ataccgttag caccagaaac ggttgcggaa cgagctgctg
    14821 tgctttgctt tgctgcggaa agtgtggcga cagattgcca gcgtgagcaa ctgagctatg
    14881 tcattggcac tgaagttccg gttccgggcg gtgaggccag cgccattcag tcagtacaca
    14941 tcacccatgt tgaagatgcc gccaatactt tacgtacgca tcaaaaggcc tttattgccc
    15001 gtgggctgac agaggcgtta acacgtgtga ttgccatcgt ggtgcagccg ggtgtggaat
    15061 ttgatcacag caatattatc cattatcagc cgcaggaagc gcaggcgctg gcgcaatgga
    15121 tagaaaacac ccgaatggtt tatgaagcac attctaccga ttaccagacc cggacggctt
    15181 attgggaatt agtccgcgat cactttgcaa tattgaaagt cggtcacgca ttaacctttg
    15241 ctttacgcga ggcgatattt gcactggcac aaattgagca ggaacttatc gcccccgaaa
    15301 atcgcagcgg ttgcctggcg gtaattgaag aagtgatgct cgacgaaccg caatactgga
    15361 aaaaatatta tcgcacgggt tttaacgatt cattactgga tattcgttac agcctgtcgg
    15421 atcgtattcg ttattactgg ccgcatagtc ggattaaaaa tagcgttgaa acgatgatgg
    15481 tgaatctgca aggcgtggac atcccactgg gcatgattag tcagtatctt cccaaacaat
    15541 ttgaacgcat tcagtccggg gaattatcag caatgccgca tcagcttatt atgaataaaa
    15601 tttatgatgt tttgcgcgcc tatcgctacg gctgtgcgga ataaggacgg tatatgacta
    15661 acctgtttgt tcgtagcgga atttcttttg tcgatcgtag cgaagtttta acctatatcg
    15721 gtaatgagat gctcgcgaaa ggtgtggttc atgatacctg gccacaggca ttaattgcca
    15781 gagaagcaga attccctacc gggataatgc ttgagcagca cgccattgca ataccgcatt
    15841 gtgaggcgat tcatgctaag tcgtcagcca tttatctgtt aaggccaaca aataaagttc
    15901 attttcagca agcggatgat gataacgacg tggcggtatc gttggttatt gcgttgattg
    15961 tggaaaatcc gcagcagcaa ttgaaacttt tacgctgttt atttggcaag ttacaacagc
    16021 ccgatatcgt cgagacacta attactcttc ctgaaactca gttaaaggaa tacttcacaa
    16081 agtatgtttt agattcagac gaataaatcc ctctgtaaca ataaaaagga ctatttatga
    16141 aacgcaagat tattgtcgct tgcggaggcg cggttgcgac ctctacgatg gcggcggaag
    16201 aaattaaaga gttgtgtcag agtcataata ttcctgttga attaatccag tgtcgggtta
    16261 atgaaataga aacctatatg gatggtgtgc atttgatatg taccactgcc agggtggatc
    16321 gtagttttgg cgatattccg ttagttcacg gcatgccttt tgtttctggt gtcggtatcg
    16381 aagcattaca aaataaaatt ctgactatct tacagggatg acctatgttt tcagaagtca
    16441 tgcgttatat tctcgacctc ggccctacag tgatgctgcc ggttgtcatc attatttttt
    16501 ctaaaatatt aggcatgaag gcaggcgatt gctttaaagc gggtctgcat atcgggattg
    16561 gctttgttgg cattggcctt gtgattggct taatgctgga ttccattggt ccggcggcga
    16621 aagcgatggc ggaaaatttc gacctgaatc tgcatgtggt cgatgtcggc tggccgggct
    16681 cttcaccaat gacctgggcg tcgcaaattg cgctggtggc gattccgatt gcgatattgg
    16741 ttaacgtggc gatgttactg acccgtatga cgcgggtggt aaatgttgat atctggaata
    16801 tctggcatat gaccttcacc ggcgcgttgc tgcacctggc aaccggttca tggatgatag
    16861 ggatggcggg tgtggtaatt cacgcggcgt ttgtttataa gctcggcgac tggtttgccc
    16921 gcgatacccg aaatttcttt gagctggaag gtattgctat tccgcacggt acgtcggcgt
    16981 atatggggcc gattgcggtg ctggtcgatg ctatcatcga gaaaatccca ggcgttaacc
    17041 gaattaaatt tagcgccgac gatattcagc gcaaatttgg cccttttggc gaacctgtca
    17101 ccgtgggttt tgtgatgggg ctgattatcg gcatcctcgc gggttacgat gtcaaaggtg
    17161 tattgcagct ggcggtaaaa acggcggcag ttatgctgct gatgccacgg atgattaaac
    17221 ccatcatgga tggtttaacg cccatcgcta agcaggcccg tagtcgttta caggcgaagt
    17281 tcggcggtca ggagttcctg attggcctgg atcccgcatt actgctgggg catacggcgg
    17341 tggtatcggc aagcctgatt tttattccgc tcaccatttt aattgctgtt tgtgttccgg
    17401 gtaatcaggt actgccgttt ggcgatcttg ccactatcgg cttcttcgtg gcgatggcgg
    17461 tcgcggtgca tcgtggaaat ctgttccgca ccttaatctc gggtgtcatc attatgagca
    17521 tcaccctgtg gatcgcgacg caaactattg gtttgcacac ccaaccggcg gctaatgctg
    17581 gggcgttaaa agccgggggt atggtggctt caatggatca gggcggttct cccattacct
    17641 ggttactgat tcaggttttc tccccgcaaa atattcccgg tttcattatt atcggcgcaa
    17701 tttatctgac cggtattttc atgacctggc gtagagcgcg tggttttact aaacaagaga
    17761 aagccgttct cgcagaataa tttttacctg agggggaggt tatccccctt aacaa
//
LOCUS       DAZSNO010000074        17730 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZSNO010000074 DAZSNO010000000
VERSION     DAZSNO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17730
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.074"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(86..1369)
                     /gene="gltA"
                     /locus_tag="ACHWN2_002380"
     CDS             complement(86..1369)
                     /gene="gltA"
                     /locus_tag="ACHWN2_002380"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR8931888.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1575..1682)
                     /locus_tag="ACHWN2_002381"
     CDS             complement(1575..1682)
                     /locus_tag="ACHWN2_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931889.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2063..2467
                     /gene="sdhC"
                     /locus_tag="ACHWN2_002382"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2063..2467
                     /gene="sdhC"
                     /locus_tag="ACHWN2_002382"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR8931890.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2461..2808
                     /gene="sdhD"
                     /locus_tag="ACHWN2_002383"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2461..2808
                     /gene="sdhD"
                     /locus_tag="ACHWN2_002383"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR8931891.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2808..4574
                     /gene="sdhA"
                     /locus_tag="ACHWN2_002384"
     CDS             2808..4574
                     /gene="sdhA"
                     /locus_tag="ACHWN2_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR8931892.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4590..5306
                     /gene="sdhB"
                     /locus_tag="ACHWN2_002385"
     CDS             4590..5306
                     /gene="sdhB"
                     /locus_tag="ACHWN2_002385"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR8931893.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5607..8408
                     /gene="sucA"
                     /locus_tag="ACHWN2_002386"
     CDS             5607..8408
                     /gene="sucA"
                     /locus_tag="ACHWN2_002386"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR8931894.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8423..9640
                     /gene="odhB"
                     /locus_tag="ACHWN2_002387"
     CDS             8423..9640
                     /gene="odhB"
                     /locus_tag="ACHWN2_002387"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR8931895.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            9734..10900
                     /gene="sucC"
                     /locus_tag="ACHWN2_002388"
     CDS             9734..10900
                     /gene="sucC"
                     /locus_tag="ACHWN2_002388"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR8931896.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10900..11769
                     /gene="sucD"
                     /locus_tag="ACHWN2_002389"
     CDS             10900..11769
                     /gene="sucD"
                     /locus_tag="ACHWN2_002389"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR8931897.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11872..12594)
                     /gene="mngR"
                     /locus_tag="ACHWN2_002390"
     CDS             complement(11872..12594)
                     /gene="mngR"
                     /locus_tag="ACHWN2_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR8931898.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12764..14680
                     /gene="mngA"
                     /locus_tag="ACHWN2_002391"
     CDS             12764..14680
                     /gene="mngA"
                     /locus_tag="ACHWN2_002391"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR8931899.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14698..17331
                     /gene="mngB"
                     /locus_tag="ACHWN2_002392"
     CDS             14698..17331
                     /gene="mngB"
                     /locus_tag="ACHWN2_002392"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR8931900.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         4314 a         4409 c         4801 g         4206 t
ORIGIN      
        1 ttacaatttt ttgtgtttag gcatataaaa attaacccgc catttgaacg gcgggttaaa
       61 atatttacaa cttagcaatc aaccattaac gcttgatatc gcttttaaag tcgcgttttt
      121 catatcctgt atacagctga cgcggacggg caatcttcat accgtaactg tgcatttcgc
      181 tccagtgggc gatccagcca acggtacgtg ccattgcgaa aatgacggtg aacatggaag
      241 acggaatacc catcgctttc aggatgatac cagagtagaa atcgacgttc gggtacagtt
      301 tcttctcgat aaagtacggg tcgttcagcg cgatgttttc cagctccata gccacttcca
      361 gcaggtcatc tttggtgccc agctctttca gaacttcatg gcaggtttca cgcattacgg
      421 tggcgcgcgg gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat
      481 catttttatc tttcgcacga cgaacaaatt ccggaatgtg tttaacggag ctaatttctt
      541 ccagcatttt cagcgccgct tcgttagcac caccgtgcgc aggtccccac agtgaagcaa
      601 tacctgctgc gatacaggca aacgggttcg cccccgaaga gccagcggta cgcacggtgg
      661 aggtagaggc gttctgttca tggtcagcgt gcaggatcag aatacggtcc atagcacgtt
      721 ccagaatcgg attaacttca tacggttcgc acggcgtgga gaacatcata ttcaggaagt
      781 taccggcata ggagagatcg ttgcgcggat aaacaaatgg ctgaccaatg gaatacttgt
      841 aacacatcgc ggccatggtc ggcattttcg acagcaggcg gaacgcggca atttcacgat
      901 gacgaggatt gttaacatcc agcgagtcgt gatagaacgc cgccagcgcg ccggtaatac
      961 cacacatgac tgccattgga tgtgagtcgc gacggaaagc gtggaacaga cgggtaatct
     1021 gctcgtggat catggtatga cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag
     1081 tcggtttttc accattcagc aggatgtaac aaacttctag gtagttagaa tcggtcgcca
     1141 gctgatcgat cgggaaaccg cggtgcagca aaataccttc atcaccatca ataaaagtaa
     1201 ttttagattc gcaggatgcg gttgaagtga agcctgggtc aaaggtgaac acaccttttg
     1261 aaccgagagt acggatatca ataacatctt gacccagcgt gcctttcagc acatccagtt
     1321 caacagctgt gtccccgttg aggttgagtt ttgcttttgt atcagccatt taaggtctcc
     1381 ttagcgcctt attgcgtaag actgccggaa cttaaatttg ccttcgcaca tcaacctggc
     1441 tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct gggtacagag
     1501 ctctgggcgc ttgcaggtaa agaatccatt gatgacgaat aaatggcgaa tcaagtactt
     1561 agcaatccga attattaaac ttgtctacca ctaataactg tcccgaatga attggtcaat
     1621 acttccacac tgttacataa gttaatctta ggtgaaatac cgacttcata acttttacgc
     1681 attatatgct tttcctggta atgtttgtaa caactttgtt gaatgattgt caaattagat
     1741 gattaaaaat taaataaatg ttgttatcgt gacctggatc actgttcagg ataaaacccg
     1801 acaaactata tgtaggttaa ttgtaatgat tttgtgaaca gcctatactg ccgccaggtc
     1861 tccggaacac cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg
     1921 aagcagtgtt ttgcatgacg cgcagttatg gaaaggacgc tgtctgaccc gcaagcagac
     1981 cggaggaagg aaatcccgac gtctccaggt aacagaaagt taacctctgt gcccgtagtc
     2041 cccagggaat aataagaaca gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca
     2101 aagacctgtt aatctggacc tacagaccat ccggttcccc atcacggcga tagcgtccat
     2161 tctccatcgc gtttccggtg tgatcacctt tgttgcagtg ggcatcctgc agtggcttct
     2221 gggtaccagc ctctcttccc ctgaaggttt cgagcaagct tccgcgatta tgggcagctt
     2281 cttcgtcaaa tttatcatgt ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg
     2341 tattcgccac atgatgatgg attttggcta tctggaagaa acattcgaag cgggtaaacg
     2401 ctccgccaaa atctcctttg ttattactgt cgtgctttca cttctcgcag gagtcctcgt
     2461 atggtaagca acgcctccgc attaggacgc aatggcgtac atgatttcat cctcgttcgc
     2521 gctaccgcta tcgtcctgac gctctacatc atttatatgg tcggtttttt cgccaccagt
     2581 ggcgaactga catatgaagt ctggatcggt ttcttcgcct ctgcgttcac caaagtgttc
     2641 accctgctgg cgctgttttc tatcttgatt catgcctgga tcggcatgtg gcaggtgttg
     2701 accgactacg ttaaaccgct ggccttgcgc ctgatgctgc aactggtgat tgtcgttgca
     2761 ctggtggttt acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag
     2821 tcagagaatt tgatgcagtt gtgattggtg ccggtggcgc aggtatgcgc gcggcgctgc
     2881 aaatttccca gagcggccag acctgtgccc tgctctctaa agtcttcccg acccgttccc
     2941 ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg taatacccat gaagataact
     3001 gggaatggca tatgtacgac accgtaaaag ggtcggacta tatcggtgac caggacgcga
     3061 ttgaatatat gtgtaaaacc gggccggaag cgattctgga actcgaacac atgggcctgc
     3121 cgttctcgcg tctcgatgat ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa
     3181 acttcggcgg cgagcaggcg gcacgcactg cggcggctgc tgaccgtacc ggtcacgcac
     3241 tgttgcacac gctttatcag cagaacctga aaaaccacac caccattttc tccgagtggt
     3301 atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt gggttgtacc gcactgtgca
     3361 tcgaaaccgg tgaagtggtt tatttcaaag cccgcgctac cgtgctggcg actggcggag
     3421 cagggcgtat ttatcagtcc accactaacg cccacattaa cactggcgac ggtgtcggca
     3481 tggctatccg cgcaggcgta ccggtgcagg atatggaaat gtggcaattc cacccgaccg
     3541 gcattgccgg tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc
     3601 tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa gacctggcgg
     3661 gccgtgatgt ggtggcgcgt tccatcatga ttgaaatccg tgaaggtcgc ggctgtgatg
     3721 gtccgtgggg gccacacgcg aaactgaaac tcgatcacct gggtaaagaa gttctcgaat
     3781 cccgtctgcc gggtatcctg gagctttccc gtaccttcgc tcacgtcgat ccggtgaaag
     3841 agccgattcc ggttatccca acctgtcact acatgatggg cggtattccg accaaagtta
     3901 ccggtcaggc gctgactgtg aatgagaaag gcgaagatgt ggttgttccg ggactgtttg
     3961 ccgttggtga aatcgcttgt gtatcggtac acggcgctaa ccgtctgggc ggcaactcgc
     4021 tgctggacct ggtggtcttt ggtcgcgcgg caggtctgca tctgcaagag tctatcgccg
     4081 agcagggcgc actgcacgat gccagcgagt ctgatgttga agcgtctctg gatcgcctga
     4141 accggtggaa caataatcgt aacggtgaag atccggtggc gatccgtaaa gcgctgcaag
     4201 aatgtatgca gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg aaagggcttg
     4261 agcagttgaa agtgatccgc gagcgtctga aaaatgcccg tctggatgac acttccagcg
     4321 agttcaacac ccagcgcgtt gagtgcctgg aactggataa cctgatggaa acggcgtatg
     4381 caacggctgt ttctgccaac ttccgtaccg aaagccgtgg cgcacatagc cgcttcgact
     4441 tcccggatcg tgatgatgaa aactggctgt gccactccct gtatctgcct gagtcggaat
     4501 ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg cccggcattc ccgccgaaga
     4561 ttcgtactta ctaatgcgga gacaggaaaa tgagactcga gttttcaatt tatcgctata
     4621 acccggatgt tgatgatgct ccgcgtatgc aggattacac cctggaagcg gaagaaggtc
     4681 gcgacatgat gctgctggat gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt
     4741 tccgccgctc ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga
     4801 atggtctggc ctgtattacc ccgatttcgg cactcaacca gccgggcaag aagattgtga
     4861 ttcgcccgct gccaggttta ccggtgatcc gcgatttggt ggtagacatg ggacaattct
     4921 atgcgcaata tgagaaaatt aagccttacc tgttgaataa tggacaaaat ccgccagctc
     4981 gcgagcattt acagatgcca gagcagcgcg aaaaactcga cgggctgtat gaatgtattc
     5041 tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat aagtttatcg
     5101 gcccggcagg cttgttagcg gcatatcgtt tcctgatcga tagccgtgat accgagactg
     5161 acagccgcct cgacggtttg agcgatgcat tcagcgtatt ccgctgtcac agcatcatga
     5221 actgcgtcag tgtatgtccg aaggggctga acccgacgcg cgccatcggc catatcaagt
     5281 cgatgttgtt gcaacgtaat gcgtaaactg taggcctgat aagacgcgca agcgttgcat
     5341 caggcaacca gtgccggatg cggcgtgaac gccttatccg gcctacaagt cattacccgc
     5401 aggcctgata agcgcagcgc atcaggcgta acaaagaaat gcaggaaatc tttaaaaact
     5461 gcccctgaca ctaagacagt ttttaaaggt tccttcgcga gccactacgt agacaagagc
     5521 tcgcaagtga accccggcac gcacatcact gtgcgtggta gtatccacgg cgaagtaagc
     5581 ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc tggttggact
     5641 cttcttacct ctctggcgca aaccagagct ggatagaaca gctctatgaa gacttcttaa
     5701 ccgatcctga ctcggttgac gctaactggc gttcgacgtt ccagcagtta cctggtacgg
     5761 gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata tttccgccgc ctggcgaaag
     5821 acgcttcacg ttactcttca acgatctccg accctgacac caatgtgaag caggttaaag
     5881 tcctgcagct cattaacgca taccgcttcc gtggtcacca gcatgcgaat ctagatccgc
     5941 tgggactgtg gcagcaagat aaagtggccg atctggatcc gtctttccac gatctgaccg
     6001 aagcagactt ccaggaaacc ttcaacgtcg gttcatttgc cagcggcaaa gaaaccatga
     6061 aactcggcga gcttatttcc gccctcaagc aaacctactg cggcccgatt ggtgccgagt
     6121 atatgcacat caccagcacc gaagaaaaac gctggatcca acagcgtatc gagtctggtc
     6181 gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag cgaactgacc gccgctgaag
     6241 gccttgagcg ttacctcggc gcaaaattcc ctggcgcaaa acgcttctcg ctggaaggcg
     6301 gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca cgctggcaac agcggcaccc
     6361 gcgaagtggt tctcgggatg gcgcaccgtg gtcgtctgaa cgtgctggtg aacgtgctgg
     6421 gtaaaaaacc gcaagacttg ttcgacgagt tcgccggtaa acataaagaa cacctcggca
     6481 caggcgacgt gaaataccac atgggcttct cgtctgactt ccagaccgat ggcggcctgg
     6541 tgcacctggc gctggcgttt aacccgtctc accttgagat tgtaagcccg gtcgttatcg
     6601 gctctgttcg tgcccgtctg gacagacttg atgagccgaa cagcaacaaa gtgctgccaa
     6661 tcaccatcca cggtgacgcc gcagtgaccg ggcagggcgt ggttcaggaa actctgaaca
     6721 tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg tatcgttatc aacaaccagg
     6781 ttggtttcac cacctctaac ccgctggatg cccgttctac accgtactgt actgatatcg
     6841 gtaagatggt tcaggcaccg attttccacg ttaacgcgga tgatccggaa gccgttgcct
     6901 ttgtgacccg tctggcgctc gatttccgta acacctttaa acgtgatgtc ttcatcgacc
     6961 tggtatgcta ccgccgtcac ggccacaacg aagccgacga gccgagcgca acccagccac
     7021 tgatgtatca gaaaatcaaa aaacatccga caccgcgcaa aatctacgct gacaagctgg
     7081 agcaggaaaa agtggcgacg ctggaagatg ccaccgagat ggttaacctg taccgcgatg
     7141 cgctggatgc tggcgattgc gtagtggcag agtggcgtcc gatgaacatg cactctttca
     7201 cctggtcgcc gtacctcaac cacgaatggg acgaagagta cccgaacaaa gttgagatga
     7261 agcgcctgca ggaactggct aaacgtatca gtacggtacc agaagcagtt gaaatgcagt
     7321 ctcgcgttgc caagatttat ggcgatcgcc aggcgatggc agccggtgag aaactgttcg
     7381 actggggcgg tgcggaaaac ctcgcttacg ccacgctggt tgatgaaggc attccggttc
     7441 gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca ccgccacgcg gtgatccaca
     7501 accagtctaa cggttccact tacacgccgc tgcaacacat ccataacggt cagggcgcgt
     7561 tccgtgtctg ggactccgta ctgtcagaag aagccgtgct ggcgtttgaa tatggttatg
     7621 ccaccgcaga accacgcacc ctgaccatct gggaagcgca gttcggtgac ttcgccaacg
     7681 gtgcgcaggt ggttatcgac cagttcatct cctctggcga gcagaaatgg ggccggatgt
     7741 gtggtctggt gatgttgctg ccgcacggtt acgaagggca ggggccggag cactcctccg
     7801 cgcgtctgga acgttatctg caactttgtg ctgagcaaaa catgcaggtt tgcgtaccgt
     7861 ctaccccggc acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg atgcgtcgtc
     7921 cgctggtcgt gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt tccagcctcg
     7981 aagaactggc gaacggcacc ttcctgccag ccatcggtga aatcgacgag cttgatccga
     8041 agggcgtgaa gcgcgtagtg atgtgttctg gtaaggttta ttacgacctg ctggaacagc
     8101 gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc tacccgttcc
     8161 cgcataaagc gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat tttgtctggt
     8221 gccaggaaga gccgctcaac cagggcgcat ggtactgcag ccagcatcat ttccgtgaag
     8281 tgattccgtt tggggcttct ctgcgttatg caggccgccc ggcctccgcc tctccggcgg
     8341 tagggtatat gtccgttcac cagaaacagc aacaagatct ggttaatgac gcgctgaacg
     8401 tcgaataaat aaaggataca caatgagtag cgtagatatt ctggtccctg acctgcctga
     8461 atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa cccggcgacg cagtcgtacg
     8521 tgatgaagtg ctggtagaaa tcgaaactga caaagtggta ctggaagtac cggcatcagc
     8581 agacggcatt ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt ctcgtcagat
     8641 ccttggtcgc ctgcgtgaag gcaacagcgc cggtaaagaa accagcgcca aatctgaaga
     8701 gaaagcgtcc actccggcgc aacgccagca ggcgtctctg gaagagcaaa acaacgatgc
     8761 gttaagcccg gcgatccgtc gcctgctggc tgaacacaat ctcgacgcca gcgccattaa
     8821 aggcaccggt gtgggtggtc gtctgactcg tgaagatgtg gaaaaacatc tggcgaaagc
     8881 cccggcgaaa gagtctgctc cggcagcggc tgctccggcg gcgcaaccgg ctctggctgc
     8941 acgtagtgaa aaacgtgtcc cgatgactcg cctgcgtaag cgtgtggcag agcgtctgct
     9001 ggaagcgaaa aactccaccg ccatgctgac cacgttcaac gaagtcaaca tgaagccgat
     9061 tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca tccgtctggg
     9121 ctttatgtcc ttctacgtga aagcggtggt tgaagccctg aaacgttacc cggaagtgaa
     9181 cgcgtctatc gacggcgatg acgtggttta ccacaactat ttcgacgtca gcatggcggt
     9241 ttctacgccg cgcggcctgg tgacgccggt tctgcgtgat gtcgataccc tcggcatggc
     9301 agacatcgag aagaaaatca aagagctggc agtcaaaggc cgtgatggca agctgacggt
     9361 tgaagatctg accggtggta acttcaccat caccaacggt ggtgtgttcg gttccctgat
     9421 gtctacgccg atcatcaacc cgccgcagag cgcaattctg ggtatgcacg ctatcaaaga
     9481 tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg atgatgtacc tggcgctgtc
     9541 ctacgatcac cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa cgatcaaaga
     9601 gttactggaa gagccgacgc gtctgctgct ggacgtgtag tagtttaagc ttcacctgca
     9661 ctgtagaccg acgtggatag ggtgtcggtc tacggtttaa aagataacga ttactgaagg
     9721 atggacagaa cacatgaact tacatgaata tcaggcaaaa caactgtttg cccgctatgg
     9781 cttaccagca ccggtgggtt atgcctgtac tactccgcgc gaagcagaag aagccgcttc
     9841 aaaaatcggt gccggtccgt gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa
     9901 agcgggcggt gtgaaagttg taaacagcaa agaagacatc cgtgcttttg cagaaaactg
     9961 gctgggcaag cgtctggtaa cgtatcaaac agatgccaat ggccaaccgg ttaaccagat
    10021 tctggttgaa gcggcgaccg atatcgctaa agagctgtat ctcggtgccg ttgtagaccg
    10081 tagttcccgt cgcgtggtct ttatggcctc caccgaaggc ggcgtggaaa tcgaaaaagt
    10141 ggcggaagaa actccgcacc tgatccataa agttgcgctt gatccgctga ctggcccgat
    10201 gccgtatcag ggacgcgagc tggcgttcaa actgggtctg gaaggtaaac tggttcagca
    10261 gttcaccaaa atcttcatgg gcctggcgac cattttcctg gagcgcgacc tggcgctgat
    10321 cgaaatcaac ccgctggtca tcaccaaaca gggcgatctg atttgcctcg acggcaaact
    10381 gggcgctgat ggcaacgccc tgttccgcca gcctgatctg cgcgaaatgc gtgaccagtc
    10441 gcaggaagat ccgcgtgaag cacaggctgc acagtgggaa ctgaactacg ttgcgctgga
    10501 cggtaacatc ggttgtatgg ttaacggcgc aggtctggcg atgggtacga tggacatagt
    10561 taaactgcac ggcggcgaac cggctaactt cctggacgtt ggcggcggcg caaccaaaga
    10621 acgtgtaacc gaagcgttca aaatcatcct ctctgacgac aaagtgaaag ccgttctggt
    10681 taacatcttc ggcggtatcg ttcgttgcga cctgatcgct gacggtatca tcggcgcggt
    10741 agcggaagtg ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca acgccgaact
    10801 cggcgcgaag aaactggctg acagcggcct gaatattatt gcagcaaaag gtctgacgga
    10861 tgcagctcag caggttgttg ccgcagtgga ggggaaataa tgtccatttt aattgataaa
    10921 aacaccaagg ttatctgcca gggctttacc ggtagccagg ggactttcca ctcagaacag
    10981 gccattgcat acggcactaa aatggttggt ggcgtaactc caggtaaagg cggcactacc
    11041 cacctcggcc tgccggtgtt caacaccgtg cgtgaagctg ttgctgctac tggcgcttcc
    11101 gcgtctgtta tttacgtacc agcaccgttc tgcaaagact ccattctgga agctatcgat
    11161 gcaggcatca aactgattat caccatcact gaaggcatcc cgacgctgga tatgctgacc
    11221 gtgaaagtga aactggacga agcaggcgtt cgtatgatcg gcccgaactg cccaggcgtt
    11281 atcaccccgg gtgaatgcaa aattggtatc cagccgggtc acattcacaa accgggtaaa
    11341 gtgggtatcg tttcccgttc cggtacactg acctatgaag cggttaaaca gaccacggat
    11401 tacggtttcg gtcagtcgac ctgtgtcggt atcggcggtg acccgatccc tggctctaac
    11461 tttatcgaca ttctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat cgtgatgatc
    11521 ggtgagatcg gcggtagcgc tgaagaagaa gcggcagcgt acatcaaaga gcatgttacc
    11581 aagccagttg tgggttacat cgctggtgtg actgcgccga aaggcaaacg tatgggccac
    11641 gcgggtgcga tcattgccgg tgggaaaggg actgcggatg agaaattcgc tgctctggaa
    11701 gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt
    11761 ctgaaataaa tatctgtaat aagaaatagc cctcgccgct tccctctaca ggaatggcga
    11821 agggctgtcg gtttcgacat ggttggccat cgtatgatgg cctttttgtg cttatcgcga
    11881 tgattttcgc tgcgctatca gggtaaattt atagtcatcg gtattaaaag cgttgcggct
    11941 atattcaaac acccgaccat caactaaata tccacgcgat actttttcaa gaatcggctt
    12001 tgtctggctg atattaagca gacggctcat ctcttcggtt ggcatcagag gaatgatttc
    12061 ctgttcgcta cgatcgataa ccatcttctt cacttcttca ataaagtgat atttcgaatt
    12121 ttccatgacc tgccaggtga gatccgggaa caacgcaagc ggcatccagg tttcttccag
    12181 cgccattggc ttttgcttgc gatagcgcac gcgcttcaca tgccaaacac gatcctgcac
    12241 ggtgatttgt agctgttgct gaagaaaatc gtcagccgga atcacttcaa atatcagaac
    12301 ttcactgtgt gtatcgacgt gacggtccga cagtttttca tcaaaactgg ttaactgaaa
    12361 aatatcgtaa ttgacccgct cttctttgac gtaagtcccg ctgccctgaa tgctttcgag
    12421 gatctgttgc tcgactaact ggcgcaaagc ctgacgcacc gtaacccggc tgacgccaaa
    12481 ctctgtttgt aatgccgatt cggtaggtaa cgcatcgcca ggtttaagct cgccacgcgc
    12541 aatttgctca cgaatgcgat cagcaatctg tcggtataag ggtttgtgtc ccattattta
    12601 gcatctcatt aatacgaatt tagccattat gcccgataaa ctcatcctgt aaacaataca
    12661 aataaaatgc aaataatttc aatcaagtga aattgattac gcaatggtat tgttttgtcg
    12721 ggcacactgg cgcgactata aaaacgatca agtgaggatc atgatgaacc tgacgactct
    12781 gacccaccgc gatgcgttgt gtctgaatgc gcgctttacc agccgtgaag aggccattca
    12841 cgcgttgact caacgtcttg ctgctctggg gaaaatttcc agtactgagc aatttctgaa
    12901 agaagtgtat cgccgtgaaa gccttggccc gaccgcctta agtgaagggt tggctgtgcc
    12961 gcatggcaaa actgctgcgg taaaagaagc ggcgtttgcg gtcgccacac tcagcgagcc
    13021 gcttcagtgg gaaggcgtgg atggcccgga agcggttgat ttagtggtgt tgctggcgat
    13081 tcccctcaat gaagcgggta ctacgcacat gcaactgctg acagcgctga ccacgcgcct
    13141 ggcggatgat gagattcggg cgcgtataca gtcggcgacg acgcctgatg aattgctctc
    13201 ggcgctggat gacaagggag gcacgcaacc ttctgcctct ttttccaacg cgccaactat
    13261 cgtttgcgta acggcctgcc cggcgggtat tgctcacacc tacatggctg cggagtatct
    13321 ggaaaaagcc ggacgcaaac tcggcgtgaa tgtctacgtt gaaaagcaag gtgctaacgg
    13381 tattgaaggg cgtttaacgg cggatcaact caatagtgca actgcctgta tttttgcggc
    13441 tgaagtcgcc atcaaggaga gtgagcgttt taacggcatt cctgcgcttt ctgtgcctgt
    13501 tgccgagccg attcgccatg cagaagcgtt gatccaacaa tcgcttaccc tcgagcgtgg
    13561 cgatgagacg cgtaccttac agcaagatac gcaaccggtg aaaagcgtca aaacggagct
    13621 gaaacaggca ctgttgagcg ggatctcttt tgccgtaccg ttgattgtcg cgggggggac
    13681 ggtgctggcg gtcgcggtat tactgtcgca aatcttcggg ctacaagatc tgtttaatga
    13741 agaaaactcc tggctgtgga tgtaccgcaa actgggcggc gggctgctcg gaattttgat
    13801 ggtaccggtg ctggcagcct ataccgccta ttcgctggca gataaaccgg cgttagcgcc
    13861 aggctttgcg gctggacttg ccgccaacat gatcggctcc gggtttctcg gcgcggtcgt
    13921 tggcggattg atagccggtt acttgatacg ctggatgaaa aatcacttgc gtcttagcag
    13981 taaattcaat ggcttcctga ctttttatct ctacccggtg ctcggtacat tgggagcggg
    14041 cagtctgatg ctgtttgtgg tgggggaacc tgtcgcctgg atcaataact cgcttaccgc
    14101 ctggctgaac ggtctgtcag gaagtaacgc gctgttgctg ggtgccattc tcggttttat
    14161 gtgttccttt gaccttggag ggccagtgaa taaagccgct tatgcattct gcctgggcgc
    14221 aatggcgaac ggcgtttacg gcccgtatgc cattttcgcc tccgtcaaaa tggtttcggc
    14281 atttaccgta accgcttcca cgatgctcgc accgcgcctg tttaaagagt ttgaaattga
    14341 gaccgggaaa tccacctggc tgttagggct ggcaggtatt accgaagggg cgatcccgat
    14401 ggcgattgaa gatccgctgc gggttattgg ttcgtttgtg ctgggctcta tggtaacggg
    14461 cgctattgtc ggtgcgatga atatcggcct ttcgacaccc ggtgctggca ttttctcgct
    14521 ctttttactt catgataatg gcgcgggcgg tgttatggcg gcaattggct ggtttggtgc
    14581 ggcattggtg ggggctgcaa tctcgactgc aattctcctg atctggcggc gtcacgcggt
    14641 taagcatggc aactatctga ctgatggcgt aatgccataa acaaaaagga aacgacgatg
    14701 aaagcggtat ctcgcgttca catcaccccg catatgcact gggatcggga gtggtatttc
    14761 accaccgaag agtcacgcat tctgctggtc aataatatgg aagagatcct gtgccgactg
    14821 gaacaggaca acgaatacaa atattacgta ctcgacgggc aaacggcgat cctcgaagat
    14881 tatttcgcgg tgaaaccgga aaacaaagac cgcttgaaga aactggtaga agccggcaag
    14941 ttgattatcg gcccctggta tacccagacc gataccacga ttgtttctgc ggaatccatc
    15001 gtccgtaatc tgatgtacgg aatgcgtgac tgcctcgcgt ttggcgagcc gatgaaaata
    15061 ggttatttac ccgattcctt tggcatgtcc gggcaactgc cgcatatcta caatggattt
    15121 ggcattacct gcaccatgtt ctggcgtgga tgttctgagc gccacggtac ggataaaacc
    15181 gagtttttgt ggcaaagcag tgacggtagc gaagtgacgg cgcaggtgat gccgttgggt
    15241 tatgccatcg gtaagtactt acctgcggac gaaaacggat tacgcaaacg cctcgacagt
    15301 tattttgacg tgctggaaaa agcgtctgta accaaagaga ttttgctgcc gaatgggcat
    15361 gatcagatgc cattgcagca aaatatcttc gaagtgatgg agaaactgcg cgaaatttat
    15421 cctcaacgaa agtttgtgat gagccgcttt gaagaggtat ttgagaagat cgaagcgcag
    15481 cgagataatc tggcaaccct gaaaggggaa tttattgatg gcaaatatat gcgcgtgcat
    15541 cgcaccatcg gttccacgcg catggatatc aaagttgccc acgcacgtat tgaaaataag
    15601 attgttaatc tgctggaacc gctggcaacg ctggccagga cgttgggttt tggataccac
    15661 cacggcttgc tggagaaaat gtggaaagag atcttaaaaa atcatgccca cgacagcatc
    15721 ggctgctgct gtcgtgacaa agttcatcgc gaaatcgtcg cccgcttcga actggctgaa
    15781 gacatggcgg ataatctgct gcgtttctat atgcgtaaaa tcgccgacaa catgccgcag
    15841 agcgacgccg acaaactcgt cctgtttaac ctgatgccct ggccgcggga agaagtgatc
    15901 aacaccactg tgcggctgcg cgccagccag tttaatctac gagacgatcg cggtcagcct
    15961 gtaccgtatt ttattcgcca tgcccgtgag atcgatccag gcctaatcga tcggcaaata
    16021 gttcattacg gtaattacga tccctttatg gagtttgata tacagatcaa ccagattgtc
    16081 ccttctatgg gctatcgcac gctttatatc gaagcgaatc agcctggcaa cgtgattgcg
    16141 gcaaaaagtg acgctgaagg gatactggaa aatgctttct ggcaaattgc gctcaatgag
    16201 gatggaacgc tacaactggt agataaagat agcggtgtgc gctatgaccg ggtgttgcaa
    16261 attgaagaaa gctctgatga tggtgatgaa tatgactatt cacccgcaaa agaagagtgg
    16321 gtaattactt cggcgaatgc gaaaccgcaa tgcgagatta ttcatgaggc ctggcagagc
    16381 agggctgtta tccgctatga aatggcggtg ccgcgtaatc tgcaacaacg cagcgcaaga
    16441 caatccactg gcagagtaga tgtcgagttg gttgtcactc ttagtcataa caacaggcgc
    16501 attgatgtgg atatcaacct tgataaccag gctgacgatc atcgccttcg cgtcctgatc
    16561 cctacatctt ttaacaccga tagtgttctg gcagataccc agtttggttc gctaacgcgc
    16621 cccgtgaacg acagtgcaat gaacaactgg cagcaagaaa gctggaaaga agcgccggtt
    16681 ccggtatgga atatgctcaa ctatgcagcc ttacaggaag ggcgtaacgg catggctgtc
    16741 tttagcgaag ggctacgtga atttgaagtc atcggtgaag agaagaaaac ctttgccatt
    16801 acgttgctgc gcggcgtggg cttactgggg aaagaagatc tgcttttaag gcctaggcgg
    16861 ccttcgggaa ttaaaatgcc agtcccggac tcacaactac gtggtctgct ttcttgtcgc
    16921 ctaagtttat tgagttatac cggtacgcca accgccgctg gtgtagctca gcaggcgcga
    16981 gcatggctga ctccagtaca gtgttacaac aaaatcccat gggatgcgat gaagctcaac
    17041 aaagccggat tcaacgtgcc ggaaagttat agtttgttga aaatgccccc agtgggatgc
    17101 ctgataagcg cacttaagaa agctgaagac cgacaagaag tgattttacg gctgtttaat
    17161 ccggctgaat cagcaacctg tgatgcgact gttgctttca gtcgcgaggt gatttcttgc
    17221 tcagaaacga tgatggatga acacattacc accgaggaaa atcaaggttc aaatctatcg
    17281 gggccttttt tacccggcca gtcacggacg ttcagttacc ggcttgcctg aatagcaatc
    17341 aaaccgaagc cacatatgcg cggccagatt gttgacaaag ggcgctttgt tcatgccgga
    17401 tacggcatga acgctttatt cggtctacaa aagcaggcaa attcaatata ttgcagagat
    17461 gatgtagaca ctggcaagcg tagcgcatca ggtaattttg tgtttatctt cactctcaag
    17521 ctacgtatat gtggctttat ttttaacaaa ataataacca cgggtgagtt aattataata
    17581 taattataag ttaattaaat gttaatatcg gcggggggga gatttatgcc tttattaata
    17641 atcctgaaac tctgcgtcgt attagccagt gaccaaaaaa agaattaagg tcaaccgtgc
    17701 tgtttttgct tcgtctcttt ttatctttaa
//
LOCUS       DAZSNO010000075        17688 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZSNO010000075 DAZSNO010000000
VERSION     DAZSNO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17688
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.075"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            84..767
                     /locus_tag="ACHWN2_002393"
     CDS             84..767
                     /locus_tag="ACHWN2_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR8931901.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            811..5473
                     /locus_tag="ACHWN2_002394"
                     /pseudo
     CDS             811..5473
                     /locus_tag="ACHWN2_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5841..7496
                     /gene="lldP"
                     /locus_tag="ACHWN2_002395"
     CDS             5841..7496
                     /gene="lldP"
                     /locus_tag="ACHWN2_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR8931902.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7496..8272
                     /gene="lldR"
                     /locus_tag="ACHWN2_002396"
     CDS             7496..8272
                     /gene="lldR"
                     /locus_tag="ACHWN2_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR8931903.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8269..9459
                     /gene="lldD"
                     /locus_tag="ACHWN2_002397"
     CDS             8269..9459
                     /gene="lldD"
                     /locus_tag="ACHWN2_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR8931904.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9655..10128
                     /gene="trmL"
                     /locus_tag="ACHWN2_002398"
     CDS             9655..10128
                     /gene="trmL"
                     /locus_tag="ACHWN2_002398"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR8931905.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10181..11002)
                     /gene="cysE"
                     /locus_tag="ACHWN2_002399"
     CDS             complement(10181..11002)
                     /gene="cysE"
                     /locus_tag="ACHWN2_002399"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR8931906.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11082..12101)
                     /gene="gpsA"
                     /locus_tag="ACHWN2_002400"
     CDS             complement(11082..12101)
                     /gene="gpsA"
                     /locus_tag="ACHWN2_002400"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR8931907.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12101..12568)
                     /gene="secB"
                     /locus_tag="ACHWN2_002401"
     CDS             complement(12101..12568)
                     /gene="secB"
                     /locus_tag="ACHWN2_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR8931908.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12631..12882)
                     /gene="grxC"
                     /locus_tag="ACHWN2_002402"
     CDS             complement(12631..12882)
                     /gene="grxC"
                     /locus_tag="ACHWN2_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR8931909.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13024..13455)
                     /locus_tag="ACHWN2_002403"
     CDS             complement(13024..13455)
                     /locus_tag="ACHWN2_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR8931910.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13700..15244
                     /gene="gpmM"
                     /locus_tag="ACHWN2_002404"
                     /gene_synonym="pgmI"
     CDS             13700..15244
                     /gene="gpmM"
                     /locus_tag="ACHWN2_002404"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR8931911.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15278..16537
                     /gene="envC"
                     /locus_tag="ACHWN2_002405"
     CDS             15278..16537
                     /gene="envC"
                     /locus_tag="ACHWN2_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR8931912.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16541..17500
                     /locus_tag="ACHWN2_002406"
     CDS             16541..17500
                     /locus_tag="ACHWN2_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR8931913.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17487..17688)
                     /locus_tag="ACHWN2_002407"
                     /pseudo
     CDS             complement(17487..17688)
                     /locus_tag="ACHWN2_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4414 a         4603 c         4639 g         4032 t
ORIGIN      
        1 tgtaaagcaa catagctcac caataataca atggacaact ctaccggcat tgagcgctgg
       61 tcggaggaaa taaggatttt tacatgaact tgaagaagat ttttttcagt gctgtcactg
      121 tttcagttct ctgcgcatta acgggatgtg actacattga agaaggcaag ccagaaagta
      181 gtttactgaa acaacaagaa gaacataata ataaaattgt gctacttgaa aaacaacaag
      241 cacagctaaa gagtcaactc gaaactattc aaaaacaaca aactggcata ataaacagta
      301 caaagacttt aactcacgta attaaatccg ttaaagacca acaaaatact tttattttta
      361 ccgagtttaa cccagcaaaa acgaagtatt tcatcctgaa caacggttcg gtggctttag
      421 cgggtcgagt gttatccatt gacgccacag agaatggtag tgttattcat atctcactgg
      481 tcaacttatt aagtacacct atctcaaaca ttggttttaa tgcgacatgg ggtggtgaaa
      541 aacctgtcga tgccaaagag tttgcccggt ggcaacaatt acttttcaac acatcaatga
      601 aatccacatt gaaattatta ccaggtcaat ggcaagacat taatttgacc ctgaagggtg
      661 tatcgcccaa taacctggga tatctgaagt tagccatcaa catggaaaat attcagttcg
      721 acaatcttcc ctctgctgaa aatcggcaga aaagaagcaa aaaataaatg cagttagtac
      781 gcttataaca aggaatagcg agcatcatta atgaacaaaa tatttaaagt tatctggaac
      841 cctgcgacag ggaattatac tgttaccagc gaaacggcaa aaagccgtgg caagaaatct
      901 gggcgcagta agctgttaat ttctgcgctg gttgcgggtg gaatgttgtc gtcgtttggg
      961 gcattggcga atgccgggaa tgacaacggt cagggtgttg attacggtag tggatcagct
     1021 ggcgacggct gggttgctat aggcaaaggg gcgaaagcaa atacttttat gaacaccagt
     1081 ggttccagta ctgctgtggg ttatgacgct atagctgaag gccaatatag ctctgccatc
     1141 gggtcaaaaa cccatgcgat tggtggtgca tcaatggcct ttggggttag tgcaatatca
     1201 gaaggcgata gaagtatagc actgggtgcc tcttcgtatt cattgggcca atactcaatg
     1261 gccctcggcc gttattcaaa agcattgggt aaattgtcta ttgctatggg ggactcttcc
     1321 aaagcggaag gagcaaacgc cattgccctg gggcgtagca gtgtagctag cggtactgac
     1381 agcctcgcat ttggcagaca atcacttgcc agcgcagcga acgctattgc gataggtgct
     1441 gagaccgaag ccgctgaaaa tgcaactgct attggcaata atgcgaaggc aaaagggact
     1501 aatagcatgg caatggggtt cggaagcctt gccgataaag tcaatactat cgcattagga
     1561 aatggcagcc aggctctggc agataatgca atcgccatag gccagggcaa caaagctgat
     1621 ggcgtggatg ccatcgctct gggtaatggt agccagtcga gaggcttaaa caccattgcc
     1681 ttaggcacag ccagtaatgc aactggtgat aagagtcttg cgcttggtag taatagcagt
     1741 gccaacggta ttaactctgt cgcgctgggc gcagattcca ttgcggattt agacaatacc
     1801 gtctctgtcg gcaatagttc attaaaacgc aagatcgtta atgtgaaaaa tggcgcgatc
     1861 aagtctgaca gttacgatgc cattaatggt tcacagcttt atgccattag cgactcggta
     1921 gcaaaaaagg cttggaggag gggctgcagt agatgttgat gacggtactg ttacagcacc
     1981 aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg gctgcgctcg ctgtacttga
     2041 tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac agcgctgctc atggtactag
     2101 tagcccaact gccagcgtaa tcaccgatgt tgcggatggc acgatttcag cctccagtaa
     2161 ggatgcggtt aacggttccc aactgaaagc taccaatgac gatgtcgaag ccaacaccac
     2221 caatatcacc aacctgacgg attccgttgg tgaccttcag gctgatgccc tgctctggaa
     2281 cgaaactaaa aaggcattca gtgcagctca cggccaggat accaccagca aaatcaccaa
     2341 cgttaaagat gccgacctga cggctgacag cactgatgct gttaacggct ctcagctgaa
     2401 aaccaccaac gatgctgtgg cgacgaatac caccaatatc gccaataaca cttccaatat
     2461 tgccactaac accaccaaca tctctaacct gactgagacg gtgactaatc ttggtgagga
     2521 tgcgctgaaa tgggataagg acaatggtgt attcacggca gctcatggca ccgagaccac
     2581 cagcaaaata accaacgtta aagatggcga cctgacgact ggcagcaccg atgccgttaa
     2641 cggctctcag ctgaaaacca ccaacgatgc cgtggcgacg aataccacca atatcgccac
     2701 taacaccacc aacatctcta atctgactga gacggtgact aatcttggtg aggatgcgct
     2761 gaaatgggat aaggacaatg gtgtcttcac tgcagctcat ggcaacaata ccgccagcaa
     2821 aatcaccaat atcctggacg gcacagtcac tgcaaccagt tccgatgcca ttaacggtag
     2881 ccagctttat gacttaagca gcaatatcgc cacctacttc ggcggcaatg cttctgtgaa
     2941 tactgacggt gtgtttaccg gtccaaccta caaaatcggt gaaacaaatt attataacgt
     3001 cggcgatgca ctggctgcga ttaactcctc atttagcacg tctctcggcg atgctctgct
     3061 ttgggatgcc accgcaggta aattcagtgc caaacacggt actaatggtg acgcaagcgt
     3121 gatcactgat gtcgcagatg gtgaaatttc agactccagt tctgacgcag taaacggctc
     3181 acaactccac ggcgtgagca gttatgttgt tgatgcgctg gggggtggtg ccgaagtcaa
     3241 tgcagacggc accatcactg cgccgacgta caccattgct aatgctgatt acgataatgt
     3301 cggtgatgcc ctgaatgcta tcgataccac tcctgacgac gctctgctct gggatgcgga
     3361 cgccggtgaa aatggtgcat ttagcgccgc tcacggaaaa gataaaactg ccagtgtaat
     3421 cactaacgtc gctaacggtg caatctctgc tgccagcagc gacgcgatta acggctcaca
     3481 actctatacc accaataagt acatcgctga tgcgctgggt ggtgacgcag aagtcaacgc
     3541 tgacggcacc atcaccgcac cgacttacac cattgcgaac gccgagtaca acaacgtcgg
     3601 tgacgccctg gatgcgcttg atgataacgc cctgctgtgg gataagactg ccaatggcgg
     3661 tgctggagcc tacaatgcca gccatgacgg taaagccagc atcatcacta atgtcgctaa
     3721 tggcagtatt agtgaggaca gtaccgatgc agtgaacggt tctcagttga atgcgacgaa
     3781 tatgatgatt gagcagaaca cccaaattat caatcagctc gctggtaaca ccgacgcaac
     3841 ctatatcgaa gaaaatggcg ctggtatcaa ctacgttcgt actaacgaca acgatttagc
     3901 ctttaacgat gcaagcgcct ctggtgtcgg cgctacagct gtaggttata acgctgtcgc
     3961 gtctggtgcc agcagcgtag ccattggtca gaacagcagc agcaccgttg ataccggtat
     4021 tgcgctgggt agcagctccg tttccagccg tgtgattgcc aaaggttctc gtgacactag
     4081 cgtaacggaa aatggcgtgg tgattggtta tgacaccact gacggcgaac tgctaggtgc
     4141 attgtcaatt ggtgatgacg gtaaataccg ccaaatcatc aacgtagctg atggttcaga
     4201 agcccatgac gccgttacgg ttcgccagtt gcagaacgct attggagcgg tcgccactac
     4261 gccaaccaag tactttcacg ccaactcaac ggcagaagac tcactggccg ttggtgaaga
     4321 ctcgctggca atgggtgcga aaactgtcgt taatggtaat gcaggtattg gtatcggttt
     4381 gaacactctg gttctggctg atgcgatcaa cggcattgct atcggcagta acgcacgcgc
     4441 aaaccatgca aacagtatcg caatgggtaa cggttctcag accacccgtg gtgcacagac
     4501 tggctacacc gcctacaaca tggacgcacc gcagaactct gtaggtgagt tttctgtcgg
     4561 tagtgaagac ggtcaacgtc agatcacaaa cgtcgcagct ggttcagcgg ataccgatgc
     4621 ggttaacgtg ggtcagttga aagtcactga tgagcgcgta gcgcaaaata cccagagcat
     4681 tactaacctg aacaatcagg tcactaatct ggatactcgc gttactaata tcgaaaacgg
     4741 tattggcgac attgtcacca ccggtagcac caagtacttc aagaccaaca ccgatggcgt
     4801 agatgccaac gcccagggta aagatagcgt tgctattggt tctggttcca ttgctgccgc
     4861 tgacaacagc gtcgcactgg gtaccggttc cgttgcagag gaagaaaata caatctctgt
     4921 aggttcttct actaaccaac gccgtatcac caacgtagct gccagtgtta atgccaccga
     4981 tgcggttaac gtgtcgcagc tgaaatcttc tgaagcaggc ggagtgcgct acgacaccaa
     5041 agctgatggt tctatcgact atagcaatat caccctcggt ggtggcaacg gcagtacgac
     5101 tcgtatcagc aacgtctccg ctggcgtcaa caacaacgac gcggtgaact acgcgcagtt
     5161 gaagcaaagc gcgcaggaaa cgaagcaata caccgatcag cggatggttg agatggataa
     5221 caaactgtct aaaactgaaa gcaagttgag cggtggtatc gcttctgcaa tggcaatgac
     5281 cggtctgccg caggcttata caccgggtgc cagcatggct tctattggtg gcggtactta
     5341 caacggtgaa tcggcagttg ctttaggtgt atcgatggtg agcgccaatg gtcgttgggt
     5401 ctacaaatta caaggtagta ccaatagcca gggtgaatac tccgccgcac tcggtgccgg
     5461 tattcagtgg taatgatcca ttaagttagt gtgactaagg gcaaacgcca aattgcctga
     5521 tgcgttacgc gcatcaggca agataaggcg ctccgggtca acaatctaag ggcaattctc
     5581 tgatgaggat tgcccttttc tttaccagac atctcccccc acaagaattg gccctaccaa
     5641 ttcttcgctt atctgacctc tggttcacaa ttccccaatt aaaactcaca tcaatattgc
     5701 caatacataa catttagtta accattcatt gtcattatcc ctacacaaca caattggcag
     5761 tgccactttt acacaacgtg tgacaaggag atgagcaaca gactcattac acgatgtgcg
     5821 tggactctag gagacctgca atgaatctct ggcaacaaaa ctacgatccc gccgggaata
     5881 tctggctttc cagtctgata gcatcgcttc ccatcctgtt tttcttcttt gcgctgatta
     5941 agctcaaact gaaaggatac gtcgccgcct catggacggt agcaatcgcc cttgccgtgg
     6001 ctttgctgtt ctataaaatg ccggtcgcta acgcgctggc ctcggtggtt tatggcttct
     6061 tctatggttt gtggcccatc gcgtggatca ttattgcagc agtgttcgtc tataagatct
     6121 cggtgaaaac cgggcagttt gacatcattc gctcgtctat tctttcgata acccctgacc
     6181 agcgtctgca aatgctgatc gtcggtttct gtttcggcgc tttccttgaa ggagccgcag
     6241 gctttggcgc accggtagca attaccgccg cattgctggt cggcctgggt tttaaaccgc
     6301 tgtacgccgc cggactgtgc ctgattgtta acaccgcgcc agtggcattt ggcgcgatgg
     6361 gcattccaat cctggttgcc ggacaagtaa caggcatcga cagctttgag attggccaga
     6421 tggtggggcg tcagctaccg tttatgacca ttatcgtgct gttctggatc atggcgatta
     6481 tggacggctg gcgcggtatc aaagagacgt ggcctgcggt cgtggttgca ggcggctcgt
     6541 ttgccatcgc tcagtacctt agctctaact tcattgggcc ggagctgccg gacattatct
     6601 cttcgctggt atcactgctc tgcctgacgc tgttcctcaa acgctggcaa ccagttcgcg
     6661 tattccgctt cggtgacttg ggggcgtcac aggttgatat gacgctggcc cacaccggtt
     6721 acaccgcggg tcaggtgtta cgtgcctgga caccgttcct gttcctgacc gctaccgtaa
     6781 cactgtggag catcccgccg tttaaagccc tgttcgcatc gggtggtgcg ctgtatgagt
     6841 gggtgatcaa cattccggtg ccgtacctca ataaactggt tgcccgtatg ccaccagtgg
     6901 tcagcgaggc tacagcctat gccgccgtgt ttaagtttga ctggttctct gccaccggca
     6961 ccgccattct gtttgctgca ctgctctcga ttgtctggct gaagatgaaa ccgtctgacg
     7021 ctatcagcac cttcggcagc acgctgaaag aactggctct acccatctac tccatcggca
     7081 tggtgctggc gttcgccttt atctcgaact attccggact gtcatcaaca ctggcactgg
     7141 cactggcgca caccggtcat gcattcacct tcttctcgcc gttcctcggc tggctggggg
     7201 tattcctgac cgggtcggat acctcatcta acgccctgtt cgccgcgctg caagccaccg
     7261 cagcacaaca aattggcgtc tctgatctgt tgctggttgc cgccaatacc accggtggcg
     7321 tcaccggtaa gatgatctcc ccgcaatcta tcgctatcgc ctgtgcggcg gtaggcctgg
     7381 tgggcaaaga gtctgatttg ttccgcttta ctgtcaaaca cagcctgatc ttcacctgta
     7441 tggtgggcgt gatcaccacg cttcaggctt atgtcttaac gtggatgatt ccttaatgat
     7501 tgttttaccc agacgcctgt cagacgaggt tgccgatcgt gtgcgggcgc tgattgatga
     7561 aaaaaacctg gaagcgggca tgaagttgcc cgctgagcgc caactggcga tgcaactcgg
     7621 cgtatcacgt aattcactac gcgaggcgct ggcaaaactg gtgagcgaag gcgtgctgct
     7681 cagtcgacgc ggcggcggga cgtttattcg ctggcgtcat gacacatggt cggagcaaaa
     7741 catcgtccag ccgctaaaaa cgctgatggc cgatgatccg gattacagtt tcgatattct
     7801 ggaagcccgc tacgccattg aagccagcac cgcatggcat gcggcaatgc gcgccacacc
     7861 tggcgacaaa gaaaagattc agctttgctt tgaagcaacg ctaagtgaag acccggatat
     7921 cgcctcacaa gcggacgttc gttttcatct ggcgattgcc gaagcctcac ataacatcgt
     7981 gctgctgcaa accatgcgcg gtttcttcga tgtcctgcaa tcctcagtga agcatagccg
     8041 tcagcggatg tatctggtgc caccggtttt ttcacaactg accgaacaac atcaggctgt
     8101 cattgacgcc atttttgctg gtgatgccga cggggcgcgt aaagcaatga tggcgcacct
     8161 tagttttgtt cacaccacca tgaaacgatt cgatgaagat caggctcgcc acgcacggat
     8221 tacccgcctg cccggtgagc ataatgagca ttcgagggag aaaaacgcat gattatttcc
     8281 gcagccagcg attatcgcgc cgcagcgcaa cgcattctgc cgccgttcct gttccactat
     8341 atggatggtg gtgcatattc tgaatacacg ctgcgccgca acgtggaaga tttgtcagaa
     8401 gtggcactgc gccagcgtat tctgaaaaac atgtccgact taagcctgga aacgacgctg
     8461 tttaatgaga aattgtcgat gccggtagca ctggcaccgg tgggtttgtg tggcatgtat
     8521 gcgcgtcgtg gcgaagttca ggcagccaaa gcggcggacg cgcatggcat tccgtttact
     8581 ctctcgacgg tttccgtttg cccgattgaa gaagtcgcgc cagccatcaa gcgcccaatg
     8641 tggttccagc tttatgtact gcgcgatcgc ggctttatgc gtaacgcgct ggagcgagca
     8701 aaagcagcgg gttgttcgac gctggttttc accgtggata tgccgacacc gggcgcacgc
     8761 taccgtgatg cgcattcagg tatgagcggc ccaaatgcgg caatgcgccg ctacttgcag
     8821 gcggtgacgc atccgcaatg ggcgtgggat gtgggcctga acggtcgtcc acatgattta
     8881 ggtaatatct cagcttatct cggcaaaccg accggactgg aagattacat cggctggctg
     8941 gggaataact tcgatccgtc catctcatgg aaagaccttg agtggatccg cgatttctgg
     9001 gatggcccga tggtgatcaa agggatcctc gatccggaag atgcgcgcga tgcagtacgt
     9061 tttggtgctg atggaattgt ggtttctaac cacggtggcc gccagctgga cggtgtactc
     9121 tcttccgctc gtgcgttgcc cgctattgca gatgcggtga aaggtgatat cgccattctg
     9181 gcggatagcg gaattcgtaa cgggcttgat gtcgtgcgta tgattgcgct cggtgccgac
     9241 accgtactgc tgggtcgtgc tttcctgtat gcgctggcaa cagcgggcca ggcgggtgta
     9301 gctaacctgc taaatctgat cgaaaaagag atgaaagtgg cgatgacgct gactggcgcg
     9361 aaatcgatca gtgaaattac gcaagattcg ctggtgcagg ggctgggtaa agagttgcct
     9421 acggcactgg ctccgatggc gaaagggaat gcggcatagt cgtttgcgcc ccctcaccct
     9481 aaccctctcc ctcagggaga ggggaccgtt cggcgctgta tgtactccct cactctgaaa
     9541 cgacaccgca ctcttttttt ctccctcgcc cctccgggga gggccggggt gaggggaaaa
     9601 ggccgcacgg ttcaccaatt ttctgctatc ctgcccccgc actaaggggg cagtatgcta
     9661 aacatcgtac tttacgaacc agaaattccg ccaaatactg gcaacatcat ccgtctttgc
     9721 gctaataccg gctttcgtct acatatcatc gaaccgatgg gatttgcctg ggacgacaag
     9781 cgcctgcgcc gcgcggggct ggactatcac gagtttaccg ccgttacgcg tcatcatgac
     9841 tatcgcgcgt tcctcgaagc agaaaatccc cagcgcctgt ttgccctcac cacgaaaggt
     9901 acgcctgctc acagcgccgt aagctatcag gatggcgatt atctgatgtt cggcccggaa
     9961 acacgcggcc tgccagcgag cattcttgat gccctgcccg ctgaacaaaa aattcgcatt
    10021 ccgatggtgc cggacagccg cagcatgaat ctgtccaatg cggtgtcggt agtggtgtat
    10081 gaagcctggc ggcagctggg atatccggga gcgttgttga gagattagtt actgtatgcc
    10141 ggatagatga ttacatcgca tccggcacga tcacaggaca ttagatccca tccccatact
    10201 caaatgtatg gttaataccg ttgaaatgct ggtccatatc cattgatggc ttatcgctgt
    10261 ctggtttacc gacaatacga gccggaacgc cagcggcggt tgtatgcggc ggcaccggtt
    10321 gcagcaccac ggaacctgcg ccaatcttcg cgccgcgacc aacttcaata ttgccgagga
    10381 ttttcgcgcc cgcgccaatc atcacacctt cacgaatttt cgggtgacgg tcaccaccag
    10441 atttacccgt accgccaagc gtcacagatt gcagaatcga tacgtcgttt tcaattaccg
    10501 ccgtttcacc cacaacgatg cccgtggcgt ggtcgagcat aatgccacgg ccaatttttg
    10561 ctgccgggtg aatatcgacc tggaacgtca cagaaacctg gttttgcaga aagattgcca
    10621 atgcgcgacg cccctgattc cacaaccagt gaccgatgcg ataggcctgc aaggcatgaa
    10681 aacccttcag gtataacaac ggggttgagt atttatcgac tgccgggtcg cgggtacgca
    10741 ccgcctgaat atcacaggcc gcagaggcga tcatttccgg gtcagcggcg taggcttctt
    10801 caaccacttc acggatagca atagcaggca taattggcga tgacagcttg ttcgccagca
    10861 tgtagctcag tgcactgcca aggttttcgt gcttgagtag cgtcgcgtgg taaaaactgg
    10921 ccagcattgg ctcacagtcc gccagcgttc tggcttcggc tttaatattg ttccagacaa
    10981 tttccagttc ttcacacgac attgcttact ccacacgatg agataatgac cggcccgttc
    11041 tgcgcgggcc gggtcatagc ggtaacaaag gttccctggg gttagtgact gctgcgctcg
    11101 tccttgcgtg cacgacctaa taatgtcaat gctgcctcgc gcgcgttttt tccgcaatat
    11161 aatacttgat aaatttcctc ggttattggc atttcaacgc cgaagcgatg cgccagttcg
    11221 cggacttctt tcgtattgcg gtagccttcc accacctgac caatcttctc ctgcgcgctt
    11281 tgtacatcca tgccctgacc gagcatcata ccaaaacggc ggttacgcga ctggttgtcg
    11341 gtacaggtaa gcaccagatc gcccagcccc gccatgccca taaaggtggc aggatcggca
    11401 cccagcgccg caccaagacg cgacatttca gccagcccac gggtgatcag cgccgtacgc
    11461 gcattcgcac caaaaccgat accgtcagac atccccgcgc caatggcaat gacgtttttc
    11521 accgcgccgc caagctgcac gccaatgaaa tccgggttgc tgtaaacgcg gaaacttttg
    11581 ccgcagtgca gcagctgctg gagatcatcg gcaaaggtct gatcggtcga ggccagcgaa
    11641 atagctgtcg gtaaacctgc cgccagttct ttcgcaaacg ttgggccaga gataaccgcc
    11701 agcggaattt gatcgcctaa ggcctcacgc gccacgtcct gtaacagacg tccggtttcc
    11761 gcttccagcc ctttggtcgc ccacaccaga cgcgcatcag gacgcatcag cggtttaatc
    11821 tggcgcagca cttcaccaaa gacatggctg ggtacgacga cgagaatatt acgactggct
    11881 gccagcgcag tggcgagatc gctttcaaga tggagcgtat cgggaaaagg cacatcgggg
    11941 agaaacgcgg cgttacagcg gtcgcgttca agcgttgcga tatgttcagg gtcatggccc
    12001 cagaggacaa cctcgtggcc atttcttgcc agggtaatgg caagagcggt gccgtacgag
    12061 ccggcaccga tcacagtcat tgaagcatta cgttggttca tcaggcatcc tgatgttctt
    12121 cagtaccttc gccagcctgc tgctgcaaat agttcatgaa cagcgcatcg aagttaaccg
    12181 gcgcaaggtt cagttgcggg aatgtaccgc gggataccat gctggtgatg cactcacgag
    12241 catacgggaa cagaatgttc gggcagtatg cgcccaggca atgtgccatc tgggtgcctt
    12301 cgatacccgc gatggagaaa ataccgccct gctgaacttc acacaggaac gcggtttctt
    12361 cgcccaagga ggccgttacg gtaacacgca gtaccacttc gtatacgtca tctgccagtt
    12421 gggaagatgc cgtatccaga tcaagtttaa cttctggttg ccaatctttc tggaaaacgt
    12481 gcggcgcgtt cggcgcttcg aaagagatat ccttggtata aatacgttgg atctggaaag
    12541 tcatttcagt gttgttttgt tctgacatgt gtagaaaacc cttaagtgtt gtccttaaat
    12601 actgcgcaat gtaataccaa cgttcacacg ttatttcagc aggggatcca gtccaccacg
    12661 tgcatccaat gcatacaagt catcacagcc gccaatgtgc tgtgcgtcaa taaaaatctg
    12721 gggaaccgtg gtgcaaccgc tgcgtttgat catctcttca cgcttggcgg cattgccatc
    12781 gatcggcaac tcctggaaac tcacgccctt gctgctcagc agtgcttttg cacgatggca
    12841 atacgggcag gtttctttgg tatagatttc aacattggcc ataatttagc tcctgttttt
    12901 ttaccctgaa atttcatgtt gcagggcggc tgcaaaatgg agtgtcccca gaagcttata
    12961 acaataagtc gctgggggcc taattgcagc taacggcctg catcatgaaa gacgacaggt
    13021 aaattatttg ccgcgcacca gaggcaagtt ttcgccagcc cagccagcaa cgccctcttt
    13081 cagcacgaat acttgcgcaa aaccagcttt cgtcagcgcg tttgcaggct cctggcactg
    13141 catgccagaa ccgtctacca cgataaccga tttgtctttg tgcttctcaa gctcaccaac
    13201 attgttggct ttgatttcgc tcggcaacag attgatagaa cctgcgatat ggcctttacg
    13261 gaagtcatca cgctgacgta aatccacaac cacagcgtct tctttgttga tcagacgcgt
    13321 agcttcacca cgagtaatca ccttcacttt cgaggtcagg ctcttaaacg tagtcacaag
    13381 aaccgccacc agtaacgcga tccaggcgat actcagtatg ggatgacggc caacaaattg
    13441 cataatttct tgcatggggg gtaacaactc ccgacgtagt gattaaaaaa accaggaaag
    13501 gagtatacct gcgcggtgcg gcaaatacag ccagcgcgtt aactggaatg caattttgcg
    13561 gggcacgacg aaaaaaaagc gcaaatctgg catcggcttg ccgcgagcgg tcgtatattt
    13621 tgatcttcag aggctatttt atcgattcag ctgtagtaaa attacgcaaa ttttgactct
    13681 tgagtatgag gttgtcgcaa tgtcggtttc taaaaaacct atggtactgg tgattctgga
    13741 tggctatggc tatcgtgaag aacagcagga taacgccatt tttagtgcta aaaccccggt
    13801 aatggatgca ctgtgggcca atcgtccgca taccctgatc gacgcttccg gtctggaagt
    13861 cggtctgcct gaccgtcaga tgggtaactc cgaagtaggc cacgttaacc tgggtgccgg
    13921 ccgcatcgtg tatcaggacc tgactcgtct ggacgttgaa atcaaagatc gcgctttctt
    13981 tgctaatcca gtgctggctg gcgcggtaca taaagcgaaa aacgcaggta aagcagtaca
    14041 cattatgggt ctgctctccg caggcggcgt acacagccac gaagatcaca tcatggcgat
    14101 ggtagaactg gcagctgaac gcggcgcaga aaaaatctac ctgcacgcat tccttgacgg
    14161 tcgcgacacc ccgccgcgca gtgctgaatc ctcgctgaaa aaatttgaag agaaatttgc
    14221 tgcgctgggt aaaggccgcg tagcgtccat cattggtcgt tactacgcga tggaccgcga
    14281 taaccgttgg gatcgcgttg aaaaagccta tgacctgctg actctggcgc agggtgagtt
    14341 ccaggccgat actgccgttg ctggtttgca ggcagcttat gctcgcgacg aaaacgacga
    14401 gttcgtgaaa gcgaccgtta ttcgtgcaga aggtcagcca gatgcggcaa tggaagacgg
    14461 cgatgcgctg attttcatga acttccgtgc tgaccgcgcg cgtgaaatca ctcgtgcatt
    14521 cgtgaacgct gatttcgatg gcttcgcgcg taagaaagtg gttaacgtcg atttcgtgat
    14581 gctgaccgaa tacgccgctg acatcaaaac tgcggttgct tacccacccg cttccctggt
    14641 taacaccttc ggcgagtgga tggcgaaaaa cgacaaaact cagttgcgta tttccgaaac
    14701 cgaaaaatat gcccacgtta ctttcttctt caacggtggc gtagaagagt cgttcaaagg
    14761 cgaagatcgc attctgatca actcaccgaa agtggctacc tacgatctgc aaccggaaat
    14821 gagctccgca gagctgaccg aaaaactggt tgcggccatc aagagcggca aatacgacac
    14881 cattatctgt aactatccga acggcgacat ggtaggtcac accggggtga tggaagcggc
    14941 ggttaaagcg gttgaagcac tggatcactg cgtggaagaa gtcgcgaaag cggttgagtc
    15001 cgtgggtgga caactgctga tcaccgctga ccacggtaac gctgaacaga tgcgcgatcc
    15061 ggcaacgggt caggcacaca cggcacacac caacctgcca gttccgctga tttacgttgg
    15121 tgataagaac gtgaaagcgg ttgcaggcgg caaactttct gacatcgcgc cgaccatgtt
    15181 gtcgctgatg ggtatggaaa tcccgcaaga gatgactggt aagccgctgt tcatcgtgga
    15241 ataatccctc cccatgaggg gaaaggcgat taataccatg acacgggccg tgaaaccgcg
    15301 caggtttgca atcaggccca tcatctacgc cagcgttctt agcgctggcg tattgttgtg
    15361 cgccttttcc gcccacgcgg atgagcgtga ccaactcaaa tctattcagg ccgatatcgc
    15421 cgcaaaagag cgcgcggtac gccaaaagca acaacaacgc gcaagcctgc tcgcacaatt
    15481 gaaaaagcag gaagaagcga tctctgaagc cacccgtaag ctgcgcgaaa cgcaaaacac
    15541 gctcaatcaa ctcaataaac agattgatga gatgaacgcg tcgattgcca aactggagca
    15601 gcaaaaagcc gcccaggagc gcagcctcgc cgcacaactg gatgccgcat tccgtcaggg
    15661 cgagcatacc ggtattcagc taattctcag cggtgaagaa agccagcgtg gacagcgttt
    15721 acaggcttat ttcggctatc tcaaccaggc gcgacaagaa accattgccc agttgaagca
    15781 aacgcgtgaa gaagtcacca tgcagcatgc tgaactggaa gagaaacaaa gcgagcaaca
    15841 aacgctgtta tatgaacagc gcgcccaaca ggcgaaactg actcaggctc tgaacgagcg
    15901 taaaaagacg ctggcggggc tggaatcttc catccagcaa ggtcagcagc agctaagtga
    15961 actgcgtgcc aacgaatccc gtctgcgtaa cagcattgcc cgtgctgaag ccgcggcgaa
    16021 agcgcgtgct gaacgagaag cgcgtgaggc ccaggcggtt cgcgagcgcc agaaagaagc
    16081 gacgcgcaaa ggcaccacct acaaaccgac cgaaagcgaa aaatcgctga tgtcccgaac
    16141 tggtggcctg ggtgcgccgc gtggtcaggc attctggccg gttcgcgggc cgacgctgca
    16201 tcgctatggc gagcagctac agggtgaatt acgctggaaa gggatggtta tcggtgcctc
    16261 tgaaggtact gaagttaaag caattgccga cggtcgagtg attctggctg actggctgca
    16321 aggttacggt ctggtggtgg tggttgagca cggtaaaggc gacatgagtc tttacggcta
    16381 taatcagagc gcactggtga gcgttggttc gcaggttcgc gcgggccagc caattgcact
    16441 ggtgggcagc agtggcggtc agggtcggcc ttcactctat ttcgaaattc gccgccaggg
    16501 tcaggcggtc aatccacagc cgtggttggg aagataagtt ttgtttccat ttcgtcgtaa
    16561 cgttcttgca tttgccgctc tgttggcgct ctcctccccc gtacttgctg gcaaacttgc
    16621 catcgtcatt gatgattttg ggtatcgccc gcacaacgaa aaccaggtgc tggcgatgcc
    16681 ttccgctatc tccgtcgctg tattacccga ttcaccgcac gccagagaga tggcgaccaa
    16741 agcgcataac agcggtcacg aagtgttgat tcatctcccg atggcaccgt tgagtaaaca
    16801 gccgctggag aaaaatacgc tacgcccgga gatgagcagc gacgaaattg agcgcattat
    16861 tcgtagtgcg gtcaataacg tgccctatgc cgtggggatc aacaaccaca tgggcagcaa
    16921 gatgacctct aacctgtttg gtatgcagaa agtgatgcag gcgctggagc gttacaatct
    16981 ttacttcctc gacagcgtaa ccatcggtaa tacccaggcg atgcgcgccg cgcaaggtac
    17041 tggcgtgaag gtgatcaaac ggaaggtgtt cctcgacgat tcgcagaacg aagcggacat
    17101 ccgtgtgcaa tttaatcgcg caattgatct ggcgcgtcgc aacggttcaa ccattgccat
    17161 tgggcatcct cacccttcaa cggtacgcgt gctacaacag atggtttata acctgccgcc
    17221 agacattacg ctggtgaaag ccagcagctt gctgaatgaa ccgcaggttg atacttctac
    17281 accgccgaaa aacgctgtgc ctgatgcacc gcgtaatccg ttccgtggcg tgaagctgtg
    17341 caaaccgaag aaaccgatag aacctgttta tgctaatcgc ttctttgaag tattaagcga
    17401 aagcatcagc cagagcacgc tgatcgttta cttccagcat cagtggcaag gctggggcaa
    17461 acagcccgaa gcggcgaagt ttaacgctag cgcaaattaa aggcgctacg cgtaatgcga
    17521 tggtgcgacc gcgttttgtc gcgccattgc cataagcgga aagaccacag taacgcctgg
    17581 taaccgacct tcacgctgcg cacattggta atcaggcgct tatacatacc cggaagtaaa
    17641 tatctcggca atcatccgtt ggcgggtaag aatatccggc tctttgcg
//
LOCUS       DAZSNO010000076        17472 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZSNO010000076 DAZSNO010000000
VERSION     DAZSNO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17472
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.076"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            53..376
                     /locus_tag="ACHWN2_002408"
     CDS             53..376
                     /locus_tag="ACHWN2_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HFR8931914.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(443..1900)
                     /gene="nuoN"
                     /locus_tag="ACHWN2_002409"
     CDS             complement(443..1900)
                     /gene="nuoN"
                     /locus_tag="ACHWN2_002409"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR8931915.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(1907..3436)
                     /gene="nuoM"
                     /locus_tag="ACHWN2_002410"
     CDS             complement(1907..3436)
                     /gene="nuoM"
                     /locus_tag="ACHWN2_002410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR8931916.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3600..5441)
                     /gene="nuoL"
                     /locus_tag="ACHWN2_002411"
     CDS             complement(3600..5441)
                     /gene="nuoL"
                     /locus_tag="ACHWN2_002411"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR8931917.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5438..5740)
                     /gene="nuoK"
                     /locus_tag="ACHWN2_002412"
     CDS             complement(5438..5740)
                     /gene="nuoK"
                     /locus_tag="ACHWN2_002412"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR8931918.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5737..6291)
                     /gene="nuoJ"
                     /locus_tag="ACHWN2_002413"
     CDS             complement(5737..6291)
                     /gene="nuoJ"
                     /locus_tag="ACHWN2_002413"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR8931919.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6303..6845)
                     /gene="nuoI"
                     /locus_tag="ACHWN2_002414"
     CDS             complement(6303..6845)
                     /gene="nuoI"
                     /locus_tag="ACHWN2_002414"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR8931920.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6860..7837)
                     /gene="nuoH"
                     /locus_tag="ACHWN2_002415"
     CDS             complement(6860..7837)
                     /gene="nuoH"
                     /locus_tag="ACHWN2_002415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR8931921.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7834..10560)
                     /gene="nuoG"
                     /locus_tag="ACHWN2_002416"
     CDS             complement(7834..10560)
                     /gene="nuoG"
                     /locus_tag="ACHWN2_002416"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR8931922.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10613..11950)
                     /gene="nuoF"
                     /locus_tag="ACHWN2_002417"
     CDS             complement(10613..11950)
                     /gene="nuoF"
                     /locus_tag="ACHWN2_002417"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR8931923.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(11947..12447)
                     /gene="nuoE"
                     /locus_tag="ACHWN2_002418"
     CDS             complement(11947..12447)
                     /gene="nuoE"
                     /locus_tag="ACHWN2_002418"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR8931924.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12450..14252)
                     /gene="nuoC"
                     /locus_tag="ACHWN2_002419"
     CDS             complement(12450..14252)
                     /gene="nuoC"
                     /locus_tag="ACHWN2_002419"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR8931925.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14346..15008)
                     /gene="nuoB"
                     /locus_tag="ACHWN2_002420"
     CDS             complement(14346..15008)
                     /gene="nuoB"
                     /locus_tag="ACHWN2_002420"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR8931926.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15024..15467)
                     /gene="nuoA"
                     /locus_tag="ACHWN2_002421"
     CDS             complement(15024..15467)
                     /gene="nuoA"
                     /locus_tag="ACHWN2_002421"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR8931927.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16098..17036)
                     /gene="lrhA"
                     /locus_tag="ACHWN2_002422"
     CDS             complement(16098..17036)
                     /gene="lrhA"
                     /locus_tag="ACHWN2_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR8931928.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4351 a         4898 c         4530 g         3693 t
ORIGIN      
        1 tccctcgtct gttttaagat gcgaaacata aaattcaatg gaggaaaaat aaatgggaat
       61 gattggctat tttgcagaaa ttgactcaga aaagattaac caactgcttg agtctactga
      121 aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac tggaatcact
      181 ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa tggagattta
      241 tccgggagtg accttttcag aagagttaga ggggcaactc tttgcctcga tcatgcttga
      301 tatggaaaag ctgattagcg cgtatcgacg tatgctgcga caagggaatc atgcgctgac
      361 ggttattgtt gggtgatggt gtcagagcgc caaaaaaaac cgccgaacat gtcggcggct
      421 ttctgactta caaactaaca gtttacatca gcggcattgc taaccgcaca atactaatca
      481 gcggttgtgg ccatacaccc agcaccagta ccaacagtgc agaaatcagc accacgatac
      541 cgcccgcgct gtactgccag tttgacggtg catcgcgacc cggttgttcc ggtgcgtgca
      601 ggtacaggct caccgccacg cgcaggtagt agtagaggcc gattgccgaa ccgacaacca
      661 cggcacccac cagccaccac aagtgtgcct ggacaccgac cgccagcacg tagaacttac
      721 cgataaagcc cagcgtcatc gggataccgg ccagagacag catcatcacc gtcatcactg
      781 ccgcgaggat cggacgatgc cagaacagac cgcggtaaga gaacagggaa tcagcatccg
      841 ggccacgata cgggctggac atcaggctga ccacgccgaa cgcgccgagg ctgctgaaca
      901 gataaccggc caggtaaacc cctaccgctt ccatcgacat ctcgccggtt tgcagcgcaa
      961 tcagcgccac cagcagatag ccgaggtgag agatagatga gtaaccgagc agacgtttga
     1021 tgttggtctg gctcaacgcc atcaggttac cgaagatgat ggaggcaaag gcgataatcg
     1081 ccagtactac gcgaatcgct tcgctgtcac ccaccggtgc gtagaggaac agacgcatca
     1141 ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa agtggaaacc ggcgcaggcg
     1201 cgccctggta tacgtctggc gtccacaggt ggaacggcac cagagagagt ttgaagccga
     1261 ggccaacaat catcatgccg aaacctgcca gcaacagcgg ctcgttgagc ataccgtcgc
     1321 cgaggttttt acccagcgcg acaaacgaca ggtcgccaga ctgcgcatac accagcgcca
     1381 taccgaacag caggaaagaa gacgctgcgg cagaaaggat ggtgtatttg atactggctt
     1441 ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc aaacagcggc aaagagatca
     1501 gttcaatacc gaggaacaga gacgccagat ggtttgcatt cgccagcagg atcccgccca
     1561 gcgcggcaat taacaccaac aggtagaact catccttgtt gtcgttatag ccttcaagcc
     1621 acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa taccagcccg gtgtaaagca
     1681 tggcgaaacc atcaacgcgc atcagcggcg taacgtccat agcgcccgcc tggccaacaa
     1741 accagagcga aaccagcgcc gcgttaagcc caataaccga gagcgtagcg ttgaggaaat
     1801 gattgcgtcg ccacgcaatg gagagcatca caaccaccac cgtcaagccg acgatcagca
     1861 acggtagcag tgcgatcagg ttttgtggag ttattgtcat ggcgatttac ggccttgtag
     1921 tagtaacgga attaacaaac cactgctgga tattgccaat cgcggagtgc gaggtatcca
     1981 gaatcggctg cggatagaag cccagcagta ccagcagcac caccagcaac aggatcataa
     2041 acagctcacg cagcgacatc cctggcagtt cctggctggc aatctggctt ttcgctttac
     2101 cgaagtaagc gcgatgcaac atcgccagcg aataaacaga tgcaaagacc agcccaaagg
     2161 tagagataac ggtaatcacc gggacaacct ggaagctgcc gaacagaatc ataaattcgc
     2221 cgacgaagtt accggtgcca ggcatcccaa gcgttgccac cgcaaagaac agcgacagtg
     2281 ctggcagcca tttcatcttg ctccacagac cgcccatcat gcgcatgtcg cgggtatgga
     2341 tacgttcata aagctgaccg cagagaataa acagacccgc cgccgacaag ccgtgcgcaa
     2401 tcatctggat taccgcgccc tggtaggcca actggctgcc ggtgtagatt gcaatcagca
     2461 cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg tttgatatcg gtctgagcga
     2521 aggtcatcca cgcaccgtag aagatgccga taacacccag ccacatagcg attggcgcga
     2581 actctgccga cgcgttcggg aacagcggca gggagaaacg caacaaaccg taagcggcgg
     2641 ttttcagcaa gatccccgcg aggtccacgg aaccggcggt cggagcctgg gagtgcgcat
     2701 ccggcagcca gccatgcagc ggaaccaccg gcattttgac tgcgaaggcg atgaagaagc
     2761 ccagcatcaa cagatattcc acaccactgg acattggcgt attcagcagc tcttcatagt
     2821 tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac cagcgccagg atggcgatca
     2881 acatcaccag accactcgcc tgggtgtaaa tgaagaactt ggttgccgcc gtgatacgcg
     2941 ttttaccgtc agaggcttta tgcccccaca gtgcgatcag gaagtacatc ggcaccagca
     3001 tcatttccca gaagaagaag aacaggaaca tgtcgatggc aaggaacacg ccgataacgc
     3061 cgcccaggat ccacatcagg ttgaggtgga agaagccctg atatttttcg atctctttcc
     3121 acgaacatag taccgccagc acaccgagca gaccggtcag cacgaccatc agcagcgaca
     3181 gaccgtcaat ggcgagatga atagagatac caaaacgcgg gatccacggc atgtcgaatt
     3241 cagactgcca ctgcggaatt ccggcggatt gcgtcagtga ataaccgccc tgcaaccaca
     3301 gttgcagcga cagcgccagc gtcaatccca tggtgatcag cgcgatccag cgtggcacct
     3361 tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc aataaaggga attaatatta
     3421 gccagggtag taacatggcg atctttattc cttgtaaaag tcccgtcagg accggatttt
     3481 caacgaattc tcacgacaga aaattctctt cggactgggg gtagtgtcgg atgcgccgcg
     3541 aaggcagctt atccgaccta cgttcttata tgcccagggg caatcccaca atccttaact
     3601 caacgcagta ccatcaacag tgccagcacc acgaccgcac cgatgctcat ggatgccaca
     3661 taccagcgca gataaccgtt ctcgcttaac agcagacctt tacctgcaaa gcgggaaaga
     3721 accgccggga tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca ggcaataccc
     3781 aggaacggct tgacgaacac tttgtcgtac agccagtcaa agccccaagc gttgtaccac
     3841 caggtactca gcaggcggcc cggcgcactg ttggcgatgg aggtcaccag agaacgttta
     3901 cccagccaca gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc agaggtaatt
     3961 tccagggtca acatgctgcc gtgcgccagt tccgtcgttt gcggaagcac gccctgcagc
     4021 ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat cagcggcagg
     4081 ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt cttttccgtg gaagacgatg
     4141 aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc tgccaccatc
     4201 agattgatat gaccattcgc catcgcaccc gcgaggatct catccttact gaagaagccc
     4261 gcagtgacca gcggcagtgc cgacagtgct gcgccgccca ccaggaagca gagataaacc
     4321 agcggaatag atttacgcag accgcccatc ttgaagatgt tctgttcgtg atggcaggcc
     4381 agaatgacgg aaccggatgc caggaacagc agcgctttaa agaacgcgtg agtcatcaag
     4441 tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta gccaatctgg
     4501 ctcatggtag agtaagcgag aacacgtttg atgtcggtct gtaccagcgc ggcaaaaccg
     4561 gccagcagca gcgtaaccgc cccgacaata cccaccagat gcagaacttc cggcgtcatc
     4621 aggaacaggc cgtgggtacg ggcgatcagg tagacacccg cggttaccat cgttgcggcg
     4681 tggatcagcg cggagacagg cgtcgggccc gccatcgcgt cagcaagcca tgtctgcaac
     4741 ggcaactgcg cagatttacc gaccgcaccg cccagcagca tcagcgtcgc ccacatcagc
     4801 atgttattgc cgtcagcaaa gtgcgctggt gccagttcca ccatatcgcg gaagttcagg
     4861 gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga ggaaaacgtc acccacacgg
     4921 gtcacgacga acgctttcat tgctgctgcg ccattcttcg gatcggtgta atagaaaccg
     4981 atcagcagat aggagcacag gcccacgcct tcccagccga ggtacatcag cagcaggtta
     5041 tcggcgagaa ccagaactac catgctggcg atgaacaggt tggtgtaggc gaagaagcga
     5101 gagtagccct cttcaccgcg catgtaccag gaggcgtaca tgtgaataag gaaacccaca
     5161 ccagtgacca ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt aaaaccgatg
     5221 ttaaagtcgc ctaccgacat ccacgtccac agcggctggc tgtatgcctg ctcgccgtta
     5281 gcgaagaaat caacgccgat aaaggcggtt accagcgccg ccaggcccac agagccaacg
     5341 ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg agaacgccag caggacgaag
     5401 ccaatcaatg gcaaaataat ggttaaggca agcatgttca tccgcgcatc tcacttactg
     5461 aatcgatgtt caggttctgg cgacgacggt gaagttgcag cagcagcgca aggccgatac
     5521 tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat cacctgaccg tcggtctgac
     5581 cccagtagct tccggcgacc acaaaggcca gcgcggaggc gttaatcatg atttccagac
     5641 caatcagcat aaacagcaga ttgcgacgga taaccagacc ggttaagcca agaacgaata
     5701 agattgccgc gaggatcagt ccatgttgta aggggatcat gcgtgctcct ccgtttttct
     5761 tttcgcgctg tcgtctttac gattgctcag cacttcaccc gcacgctctt cacgaccgac
     5821 gtggaaggcc acaaccagac ccgcgagcag cagcatagaa gccagttcca ccgccagtac
     5881 gtaaggcccg aacagcgtaa tacccactgc tttagcactg attggcgtac cgtcgatacc
     5941 ctgatcgtta acaccgagga tggcgtaaac aatcaccacc agcatgatgg ccgacaaaat
     6001 tgccgggcca atccacacct gcggtttcag ccactggcgt tcctgttcga tttctgaacc
     6061 gcccaggttg agcatcatca ccacgaacac gaacagcacc ataatggcac ccgcgtagac
     6121 gataatttcc agcgcaccgg cgaagtaagc gcctagtgag aagaacaccc cggaaatcgc
     6181 cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga ttggtatggg tgatcactcg
     6241 caaggtcgca agtatggcta tcaggccaca gatataaaaa gcgaactcca ttgcccctct
     6301 ccttacggta acaggctctt gacgtcgata ggcttggctt cgttctctgc ttcgccctta
     6361 tctttgccgt cgattgccat acctgccatc cggtagaagt tatattccgg gtatttgccc
     6421 ggaccggaga tcagcagatc ctctttctcg taaaccagat cctggcgctt gtattccccc
     6481 atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc gcacagacca
     6541 cagaaaatgc agcgtgagaa gttaatgcgg aaaaattccg gataccagcg accgtctttg
     6601 gtttctgctt tttgcagcga gatacagccg accgggcagg ctaccgcaca gaggttacag
     6661 gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc acgataacgg
     6721 ggcggcagat agaccggctc ttccgggtac attcgcgttt cgcgtttggc gaacgcgtgc
     6781 aggccgatca tccagatact acgaacctgg gtgccgaaac ctactaacaa ttcttttaag
     6841 gtcatggtct tattgcccct tattgcgcct gccagagaat gacagccgcc gttaccagca
     6901 agttgatcag cgtcagcggc aggcagattt tccagccgaa ggacattacc cggtcataac
     6961 gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat cataaagaac gcggttttca
     7021 gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca gccaccgaag aacagcgtca
     7081 ccatcaatgc agagatggtc acaatcccga tgtactcacc cacgaagaac agaccgaact
     7141 tcataccgga atattcaatg tggtaaccat ccgccagttc ctgctcggct tccggctggt
     7201 caaacgggtg acggtgacac accgccacgc ccgcgatggc aaaggtaata aaaccaaaga
     7261 attgcgggat gacgttccac acatgcgcct ggctgttgac gatgtcggtc atgttgaatg
     7321 aaccggcctg cgccaccacg cccatcaggg aaagcccgag gaacacttcg tagctcaggg
     7381 tctgcgcaga agcacgcatc gcacccagca gcgagtattt gttgttactt gaccagcccg
     7441 caaacagcac cgcgtaaacc gccagacctg ccatcatcag gaagaacaaa atcccgatgt
     7501 tcaggtcggc aaccacccaa cccggactga ctggcacaat tgcaaaggcc agcagcagcg
     7561 aggtaaaggc aatcatcggt gccagggtaa agatgacgcg atccgagaat ttcgggatcc
     7621 agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag ctggagcgaa ccgccccagc
     7681 caacacggtt aggtccataa cggttctgga acagacccag caggcgacgt tcgccaaagc
     7741 tcatgaatgc cccacaggtg acaaccacca gcaggatcac caccgctttg aggatggtca
     7801 gcaggatctc aatcagttcc ggtgatatcc aactcattgt tgtgcctcct tgagatcctc
     7861 aagatgcgcg ccagccagca ccggagcaat gccggacata cccatcggca agcccacctg
     7921 ccctgccgtc agtccttcgg cgatttcaac cggcagcgtg accgtgttgc catcgtaact
     7981 aaaggagacg cgtgtacctg cgttcacacc caacttcgcg gcatccgctg ggttgagttt
     8041 gatgtacggc tgcggcatac ggctctggaa gaccggagca cgctgtgaca attcatcgct
     8101 gccaaacagg tggtagtacg gcgcgatacg ccatttcccg tcctgcggct ggaagcgtgc
     8161 cggtacgctg gtgaagtaat ccagaccgtt ttcgctggtt tcaaacagac gcacgcccgg
     8221 atcgccaaag cgcagtttgc cgcccacttc gtcctggaat ttgttccacg cctgcgggga
     8281 gttccagccc ggcgcccagg caaacggcac ttgcgaacgg tgcgcggtcg gctggttgtt
     8341 accttccatc gagaaggtga acatggtgtc aatatcctgc ggctgacgcg gctcatgaac
     8401 gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac gggccagttt
     8461 ctgcccacga atacggaatg tcgcatccgg cgcagcatct ttgatacccg ccagttctgg
     8521 gattttcgcc acaacagcgt caattacatg gtcgagctgc gtccagtcca cttcacggct
     8581 cagcaaggtg ctgtgcagcg agtgcagcca gcgccagctt tccagcatga cagttttgct
     8641 gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg gcgcgacctt cattattaat
     8701 taccgtaccg tcgctttcag caaagctggc agaggaaagc accagatggg cgttttccat
     8761 gatcgctgtg cgttgatgat caaccaccat caccagcggc gctttagcca gcgcagcatt
     8821 cacgcgggta gcagaagcgt aacgatgcag atcgttttcc aacaccacca ccgcgtcggc
     8881 gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgaa ccgccaccca taatgcccag
     8941 ccccatgctg ttgacggaac gggcaatcat ggtgatacca acgtcagcgc cgcgaccttt
     9001 cagggctttc gcgacgttag ccgcagcctg aatcacctct atgctaccgg cgttcgtccc
     9061 ggagataatc aacggtttct tcgcacctgc cagtgcctgc acgatgacgt cgattttgtt
     9121 ttgcagctca ggttcgatac cgtcaaccgc tggtgcggag ttatccagcg catgggcgat
     9181 ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg ccgcgatatc
     9241 atccagacga gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac gttgaccgat
     9301 gttgaggatt gccgcaatct gccagtcagc cactttctgt gctgcagcca tttcgcgcgc
     9361 tttacctttc accgcctgac gcactgccag tgcgacgcgc gcgccggtct gggtaacgtc
     9421 ttcgcccagc accagtaccg catcgtaaga ttcgatttcg cgcagagccg gagtataaat
     9481 gccgccttca cgcagcactt tcagcgccag ttgcagacgt tcctgctcac cgtgagcgat
     9541 accggtgtag aagttttctt cgcccaccag ttcacgcagc gcaaagttgc tttccacgct
     9601 ggcacgcgga gaaccaatac cgatcacttt cttcgactga cgcagaatat ctgccgcgcc
     9661 ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg ccacgacgct gtactggctg
     9721 acgcggacga tccttcaggt tgacgtaacc gtaaccgaaa cgaccacggt cgcagaggaa
     9781 gtagtggttt accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc cgtaacgttc
     9841 accggggctg atgttacagc cgatggaaca ttgctggcag atgctcggcg caaactgcat
     9901 atcccattta cggttgtaac gctcggagtg cgttttgtcg gtaaatacgc cggtcgggca
     9961 aatttcgacc aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg ggcgaccgaa
    10021 gtagacgttg tcgtgcgcgc cataaacgcc cagatctgta ccgtcagcgt aatctttgta
    10081 gtaacgcaca cagcggtagc aggcgatgca gcggttcatt tcgtgagaga tgaatggccc
    10141 caaatcctga ttacggtggg tacgtttggt gaaacggtag cgacggaagc tgtgtccggt
    10201 catcacggtc atatcctgaa gatggcagtt accgccttct tcacataccg gacagtcgtg
    10261 cgggtggttg gtcattaacc actcgaccac actttcacgg aactgtttcg cttcttcgtc
    10321 gtcaatggaa ataaaggtgc catcggaagc cggtgtcata caggacatca ccaggcgacc
    10381 acgcgtgtct tccgcgtttt ggtattgctt caccgcacac tggcggcaag caccgacgct
    10441 tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg cccagagaca gacaagcttc
    10501 cagcaggttg tccgctccgt tgacctcgta ttctttgccg tctacatgaa ttgtagccat
    10561 tagcatgctt ccagttttct cggtcaggga ccgagcgtta atcgaaattc ggttaccagc
    10621 gctctttcag caggttcggc tgaatcccat taatcaaatg ggtattgctg aacggctgtt
    10681 tgattcccgc ctcaaattct tcgcggaaat atttgatggc gctctgtaac ggctccactg
    10741 caccaggtgc gtgggcacag aaagttttac ccgggcctaa gaatcgacac agttgctcaa
    10801 gtgtttcgat atcgcccggc tggccttcgc cacgctccag cgcacgcaga attttcacgc
    10861 tccacggcag accgtcgcgg cacggcgtac accagccgca ggactcacgg gcgaaaaact
    10921 cttccaggtt acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc atcgccagcg
    10981 ccgtacccag acggctgccc gctttaccga tactttcgaa ttccatcggc agatcaaggt
    11041 gcgcttcggt caggaagtca gtccccgcgc cgcctggctg ccaggcttta aatttcagac
    11101 catcacgcat accaccggcg taatcttcga ggatctcgcg tgcggtggtg ccgaacggca
    11161 gttcccacag tcccggattt ttcacccgac cggagaagcc catcagcttg gtgccagcat
    11221 ctttactttt cgagatgttc tgataccact ccacgccgtt agcgaggatc gccggaacgt
    11281 tacacagggt ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg gaggttgccg
    11341 ggaagggggg tttcgagcgt gggttagcac gacgcccttc cagggagttg attaacgctg
    11401 tttcttcccc gcagatgtag cgccctgccc cggtatggac gaacagttcg aagtcgaaac
    11461 ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt ggcttcggca atggcgcggc
    11521 gcagatttac tgccgcttcg atatattcgc cacgcaggaa gatgtagcca cggtaagctt
    11581 tcagcgcaaa cgcggagatg agcatacctt ccaccagcag gtgcggcagt tgctccatca
    11641 acaggcggtc tttataggtg cccggctcca tttcatcggc gttacacagc aggtaacgga
    11701 tgttcatgga ttcgtctttc ggcatcaggc tccacttcag gccagtcgaa aagcccgcgc
    11761 cgccgcgccc tttcagacca gcgtctttta cctgattaac gatttcgtcc ggagacagcc
    11821 cggtcagcgc cttacgcgcc ccttcgtaac cgtttttgct gcggtattcg tccagccaca
    11881 ctggctgttt gtcatcgcgc agacgccagg tcagcggatg cgtttcggga gtacggataa
    11941 tgtttttcat ttataccgct ccagcagttc agggatcgct tccggggtca gatgcgcgtg
    12001 agtgtcctca tcgatcatca tgtttggccc tttatcacag ttccccaggc agcaagttgg
    12061 cagcagcgta aagcggccat caaatgtcgt ttgccctggt ttgatgttca gctttttctc
    12121 gagcgccgcc tgaataccct gatacccgtt gatatgacag accacgctgt cgcaataacg
    12181 gatcacatgg cgaccaaccg gctggcggaa gatctgactg tagaacgttg ccacaccttc
    12241 gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg tggatcgcac catccggcac
    12301 ccagccacgc tgcttctgaa cgattttcag cgcttcaatg gacgccgcac gcgggtcttc
    12361 gtagtggtgc atctcgtgct caatcgcttc acgctctgcc gcactcagct caaaagcctc
    12421 ggtttgtggt tgttgattct cgtgcataat tagcggtcca catctgacat aacaaaatcg
    12481 atactgccca gataaacaat caggtcagac accaggctgc cgcggatcgc cgccggaatt
    12541 tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc gataactcat ggtgctaccg
    12601 tcgctggtca ggtagtaact gttgatcccc ttggtcgcct caatcatctg gaaagattca
    12661 ttggcaggca tcaccggccc ccacgacact tgcaggaagt gggtgatcag ggtttcgata
    12721 tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc tttgaacggg
    12781 ccttccggca tgttgttgag gcactgctca agaatgcgca gactctggcg cagctcttcc
    12841 actttcagca ttacgcgggt gtagcagtca gaaacaccac caccaaccgg gatttcaaag
    12901 tcgaagtttt catagccaga ataaggacgc gccttacgca cgtcgaagtc gatcccggta
    12961 gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct ctttcgcgcc ataggcggca
    13021 acgccctggg aacgaccttt cagaatggta ttttgcagcg ccgctttctc gtaagacgcc
    13081 agacgtttcg gcatccagtc gaggaactca cgcagcagac gatcccagcc gcgcggcagg
    13141 tcgtgcgcta cgccgccaat acggaaccac gccgggtgca tacggaaacc cgtgatcgct
    13201 tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga agaacacggg cgtcattgcg
    13261 ccgacgtcct gaataaaggt cgagatgtac agcaggtggc tgttgatacg gaacagttcg
    13321 gagagcataa cgcgaatgac gttaacgcga tccggcacgg tgatcccggc cagtttctct
    13381 accgccagca cgtaaggcat ttcgttaacg cagccgccga ggtattcaat acggtcggta
    13441 tacggaatgt agctgtgcca ggactggcgc tcgcccattt tctccgcacc acggtggtgg
    13501 tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt cgagttgtaa aacgatacgg
    13561 aaagccccgt gcgccgacgg gtggttcgga ccgaggttga ggaacatgaa gtcctcattt
    13621 tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg tcagggcttc catctccaga
    13681 tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg tagcgcgtgc cgggtaatct
    13741 ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag gttcgggtga
    13801 ccgtcgaaag taatgccaaa cagatcccag gtttcacgct cataccagtt agcgttcggg
    13861 aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac cttcagcatg
    13921 atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc cgcggcaggt
    13981 aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa cagcatgacg
    14041 taaggtttcg gcagtttctt taagaaatcg ccaacttcca gtaattgttc acgcttgatc
    14101 cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg cccaaaacgg
    14161 ttgcgcagtt cgccaattac cggatcatca agatgatcgc gggtctgcca ggcgggttct
    14221 tgcgcggtta agtcggtcat attgttcacc attgcaaatg gtccgtggtg actgtcggcg
    14281 cagtaagctt cgcgaaatag atttaggaat aagcgaagaa aaatcccttt gccgacaggc
    14341 gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg caatgcgttc accgcgcttg
    14401 cgttcgcgct ctgattgcat attggcgcga taaacgccct gatcgccaac cacccaggag
    14461 agcggacgac gttctttgcc gatagattcc tgcaacagca tcagtgcctg catgtacgct
    14521 tcaggacgcg gcgggcagcc cgggatatac acatcaaccg ggatgaattt atcgacgccc
    14581 tgcacaacgg aataaatatc gtacatacca ccagagttgg cacaggcacc cattgagata
    14641 acccattttg gttccagcat ctggtcatac agacgctgaa taaccggtgc cattttggta
    14701 aagcaggttc ctgcaaccac catcaggtca gcctgacgcg gcgaagcacg caatacttct
    14761 gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc aacgtaacag
    14821 caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt aaccatgtca
    14881 ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc ggttacgatc
    14941 tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg ggtgagcgta
    15001 taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt cgtttccggg
    15061 ttcatacgct cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat acgcaccaga
    15121 taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa gcctacccag
    15181 ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac gtcgaagata
    15241 acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc ggagccgacc
    15301 gagtcgatac cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg accgcctaaa
    15361 aaccaaccgc ctaccagcat caggcaacac aggccaatgg caacgataag aaagatagcg
    15421 aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc ttactcatca
    15481 aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga ttcatgggga
    15541 ggaataaaaa aaaccttaca ataactgtag aaattctttt atacagctaa ttgatgtggt
    15601 cttttactcc tttctataac cttttgtcaa ctttaacaaa agcttcttca cattagttta
    15661 cataatatca acaccattag catttaatgc cctttcaccc cagatccttg acgactccag
    15721 gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat acgggtctat
    15781 tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt gatctgtgac
    15841 acgcttttgt tattccataa caaaaacgca gcaacaaatt tacgtatttt ttaacatcat
    15901 tgtagcaggt gatttttttc aggcgattat ttgtgcattc gggacgtgaa tctctggtga
    15961 ttgaaaaata aacagttttg tacgttctgc actatgcgag gaaggatttt tactaaaaaa
    16021 aagccgctgg ggtttaaaac acccccagcg gctcgttttt tacactattg tctcaggaat
    16081 tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc atccccttcc
    16141 ggagcagaca ttgggctgta ttgccacgga ttatggtagc tctccatcgc ctgatagatc
    16201 acctgggcca gctcattatt actggacggg tcatagcaca gcaaatattc ggtatcaggt
    16261 agcggcggta agccatctac tccactcagc acacgcaggt ccgggctcat catctcaacc
    16321 ggccttgccg tcacgccaag accggctttc actgccgcac gaacggccgg aagcgtcgag
    16381 gcgacataag ccagtcgcca tggaatatct actttattaa gcgtcgccag caccatatcg
    16441 cgaaacggac ttggatcatc cagcaataca agagggatcg gctcgccttt ttgcaatacg
    16501 tattccgctg cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc cttaaacgct
    16561 gaaggacgat gggtggttac catcaaatcc acttcctggg attccagcat ttctgccata
    16621 taggcattac gtttaacgcg gacatccagc gctaatttcg gataaaccga actcacgcga
    16681 tttaacagga aaggtaagat cgtatcggca gattcatctg aagcaccgat agttaacacg
    16741 ccctgaagat tactgaacat taatgatgag caggcctcat cattaaaacg caggattttc
    16801 ctggcgtaac caagaagttg aatgccatgt tcagttaaca gtttgttgcg accgtgacga
    16861 gcgaacagtt ctttcccaac gagttgttcc agacgctgca tttgctgact tacggcggac
    16921 tgagtacgac acacagcggc agctgcggca gcaaaagtgt tcagatcggc aacagcaaca
    16981 aatgttctca gcagatcgag gtcgaggtta attatcggac gatttgcact tatcatatat
    17041 tatcacttac tggcggctca tactgagctg gttaatgctg tgcacacaca aacaagcaat
    17101 tccatttgta atgtgcctcc ctggcagttt catccggaac ccggacgaaa gtaaaaatgc
    17161 atatgagttg cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg aagtagatca
    17221 cagaatatat cttcagggat cgcatatcta ttaagttact cactcttttc tatttatgac
    17281 atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc cactctcgtg cagcatcgct
    17341 ggtcatacgc gaacacgtac aaacagcaat ggtgaagcgg catcaagagc agggatccgt
    17401 catttatgcg agcattttac cccaaaaacc ttttatttat aagggtcatt gcgaattatc
    17461 tgatgcaaag tt
//
LOCUS       DAZSNO010000077        17391 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZSNO010000077 DAZSNO010000000
VERSION     DAZSNO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17391
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.077"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(43..774)
                     /gene="rlmB"
                     /locus_tag="ACHWN2_002423"
     CDS             complement(43..774)
                     /gene="rlmB"
                     /locus_tag="ACHWN2_002423"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR8931929.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(954..3395)
                     /gene="rnr"
                     /locus_tag="ACHWN2_002424"
     CDS             complement(954..3395)
                     /gene="rnr"
                     /locus_tag="ACHWN2_002424"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR8931930.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3434..3859)
                     /gene="nsrR"
                     /locus_tag="ACHWN2_002425"
     CDS             complement(3434..3859)
                     /gene="nsrR"
                     /locus_tag="ACHWN2_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR8931931.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4064..5362)
                     /gene="purA"
                     /locus_tag="ACHWN2_002426"
     CDS             complement(4064..5362)
                     /gene="purA"
                     /locus_tag="ACHWN2_002426"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR8931932.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5466..5663)
                     /locus_tag="ACHWN2_002427"
     CDS             complement(5466..5663)
                     /locus_tag="ACHWN2_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR8931933.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5745..6749)
                     /gene="hflC"
                     /locus_tag="ACHWN2_002428"
     CDS             complement(5745..6749)
                     /gene="hflC"
                     /locus_tag="ACHWN2_002428"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR8931934.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6752..8011)
                     /gene="hflK"
                     /locus_tag="ACHWN2_002429"
     CDS             complement(6752..8011)
                     /gene="hflK"
                     /locus_tag="ACHWN2_002429"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR8931935.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8097..9377)
                     /gene="hflX"
                     /locus_tag="ACHWN2_002430"
     CDS             complement(8097..9377)
                     /gene="hflX"
                     /locus_tag="ACHWN2_002430"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR8931936.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9453..9761)
                     /gene="hfq"
                     /locus_tag="ACHWN2_002431"
     CDS             complement(9453..9761)
                     /gene="hfq"
                     /locus_tag="ACHWN2_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR8931937.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9847..10797)
                     /gene="miaA"
                     /locus_tag="ACHWN2_002432"
     CDS             complement(9847..10797)
                     /gene="miaA"
                     /locus_tag="ACHWN2_002432"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR8931938.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10790..12637)
                     /gene="mutL"
                     /locus_tag="ACHWN2_002433"
     CDS             complement(10790..12637)
                     /gene="mutL"
                     /locus_tag="ACHWN2_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR8931939.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12647..13984)
                     /gene="amiB"
                     /locus_tag="ACHWN2_002434"
     CDS             complement(12647..13984)
                     /gene="amiB"
                     /locus_tag="ACHWN2_002434"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR8931940.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14003..14464)
                     /gene="tsaE"
                     /locus_tag="ACHWN2_002435"
     CDS             complement(14003..14464)
                     /gene="tsaE"
                     /locus_tag="ACHWN2_002435"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR8931941.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14436..15983)
                     /gene="nnr"
                     /locus_tag="ACHWN2_002436"
     CDS             complement(14436..15983)
                     /gene="nnr"
                     /locus_tag="ACHWN2_002436"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR8931942.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKPLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15982..17121
                     /gene="queG"
                     /locus_tag="ACHWN2_002437"
     CDS             15982..17121
                     /gene="queG"
                     /locus_tag="ACHWN2_002437"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR8931943.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17104..17157)
                     /locus_tag="ACHWN2_002438"
     CDS             complement(17104..17157)
                     /locus_tag="ACHWN2_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931944.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3945 a         4957 c         4368 g         4121 t
ORIGIN      
        1 aaaaccatcc aaatctggat ggcttttcat aattcagagg aactagctgc gctgacgaac
       61 cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agggaagaaa cacttcctgc
      121 cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca taccttcacc
      181 ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctctgataga gtgtatgatc
      241 cgcctcgcct gccgtaccga cgatccagat attctcttcc tgcaacatac gcatggtgcg
      301 cgcaaggtta gtcacccgaa tcagcggaac gctttctgcc gcgccgcagg ctactttttt
      361 cgccgtagcg ttgagctgtg cggagcgatc tttcggcaca atcaccgcat gaacgcctgc
      421 ggcgtccgca ctacgcaggc acgcgccgag gttgtgcgga tcggttacgc cgtcgaggat
      481 cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt cctgatactg
      541 acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc ttttctcgtc
      601 gagatattgg cggtttgcca actggataac cacgccctga gactcaaggg cgtgaatcag
      661 cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa aacgttcagg
      721 ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa atcatttcgc tcattaatgt
      781 actcgttgtt tacgttttgc ctgatgcact acgtttatcc ggcctacatg atctctgcaa
      841 tatattgaat ttgcgtgatt atgtaggccg gataaggcgt tcacgccgca tccggcatga
      901 acaaagcgca ttttgtcagc aatctaaccc tcttctttta aagagggtat tgatcactct
      961 gccacttttt tcttcgccgc acgcttcgct ttggtcgctg cggctatttt ctgcgttttc
     1021 gccgatggct ttttcgcttt tctcgcgtct ttcttcgccg ctttcggctt cgtttttttc
     1081 tcaccgcgga aggcgctgtc tggctcaaag tttacctttt taccgacctg acaacgcttg
     1141 ccgccttttt tgcctgcatc gccttttttc gctttctcgc gcgccgtttt accgacgttg
     1201 cgcggtgcgc gttcactgga gatcagacta aagtcgattt tgcgctcgtc catattaacc
     1261 gcttcgacgc gaacttccac gcgatcgccc aggcgataag tctggccgct ggattccccc
     1321 atcaggcgtt gccctacctg gtcaaagcga tagtagtcat tgtccagcga agagacatgg
     1381 accagaccat caatgaacaa gtcgtccaga cggacgaaga agccaaagcc agtgacgctg
     1441 gaaattacgc ctttaaagac gttacctacc tggtcgagca tgaagtcaca cttcagccag
     1501 tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg ccatcgaaca gtgctgaccc
     1561 agttgcaaca tctcttccat cgaataatgg tagccgccgg tttcagtggt gttgccctga
     1621 tgcccctgct ctttcgccag cagatattta atggcgcggt gcagcgtcag gtcaggataa
     1681 cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg ccagaccgaa gtgaccacgg
     1741 ttttctggat cgtaaatcgc ctgtttcatc gagcgtagca gcatggtttg cagcatttct
     1801 gcatcaggac ggtcggcaac cgactccagc agttccgcgt agtcacgcgg ttccggcttg
     1861 ttaccacccg gcagctccag ccccagctcc gccagcactg aacggaaaga ggtaatcgct
     1921 tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt ctttcgcttt ctcaacgaaa
     1981 cgcgccgccg agatattcgc cagaatcatg cactcttcaa ttaatttgtg cgcgtcgtta
     2041 cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa cttcgcttct
     2101 tcgctctcaa atgagatccc accgcgttct tcacgggctt tatccagcac tttatagagg
     2161 ttatgcaact cttcgagatg cttaaccagc ggggcgtact gctcgcgcag atcctgatcg
     2221 ccctgcagaa tatgccagac tttggtgtag gtcagacgcg cgtgagagct catcaccgct
     2281 tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa ccgtcatctc gcacaccata
     2341 cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt tagagagcac ttccggcagc
     2401 atcgggataa cctgcgaagg gaagtacacc gacgtgccac ggttacgcgc ttctctgtcc
     2461 agcggcgttg gcggacgcac atagtagctg acgtcggcaa tcgcgaccca taaacgccag
     2521 ccgccgccgc gttttttctc gcagtaaact gcatcgtcaa agtcacgggc gtcttcgcca
     2581 tcaatggtga ccagcggtaa atcgcgcaaa tcgacacggc ccgcttttgc ttcttccggc
     2641 acttcttctt tcagccccgc aacctgttgc tcaacagcct gcggccagat atacggaatt
     2701 tcatgggtac gcagagcgat atcaaccgcc atgccggtgc ccatattgtc gcccagcact
     2761 tcgacgattt tacccaccgc tttggtgcgg cgagtcggac gctgagtcag ttcgactacg
     2821 accacaaagc ccatccgcgc gcccatgatc tgatcgggcg ggattaagat atcgaagctc
     2881 agacggctat cgtcaggaac cacaaagccg acgcccgctt cggtaaagta gcgaccaaca
     2941 atctggctgg tttttggcac cagtacgcgg acaatacgcg cttcacgacg acctttacgg
     3001 tcagcaccca gcggctgcgc cagcacctga tcgccatgaa tgcaggtttt catctgctcg
     3061 ctggagagat acaaatcatc tttacgccct tcaacccgca gaaagccgta gccatcacgg
     3121 tgaccaataa cggtaccttt caccaggtcg aggcgttccg gcagcgcata gcactgacga
     3181 cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca ggcggcgacg caggccttca
     3241 agctgctctt cgccttcaat gtgcagttct accgccagct catcacggct ggccggtttt
     3301 tcacgtttgg ttaaatgttc gaggataaat tcccgactag ggatgggatt cgcgtatttt
     3361 tcagcttcgc gttcctggaa aggatcttgt gacatctcgg ttcctccgtt gtcatctctg
     3421 atgaagattt tcgtcactcc accagcaata atttataaag cggttgattc tcttcaacca
     3481 aatcggcaag cgtgtagtta tccagttccg taagaaaact ttgcacggcc ttagaaagcg
     3541 cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc actgctgcaa ttcaccagcg
     3601 ataagggctc cagctcgcgc accacatcac caatacgtat cgcactcgcc ggtttaccca
     3661 ggcgaatgcc gccatttttt ccacgaacag cagtcacgta gccggcacga ctaagttgat
     3721 tgattatttt gaccatatga ttacgggaga cgccgtagac gtcagtcact tcagaaatac
     3781 tggtcatccg cccttctggc aatgacgcca tgtagatcag cgcacgtaat ccgtaatcag
     3841 tgaaactcgt taactgcaca tcaacctcaa aagggaaatc gggaaaaaat acatttatat
     3901 tgatgataaa ccagccacaa gctgtgtcgc taatttattt cagttcgggg aaggaaaaaa
     3961 gcgaggattt aacactgtgt cggatagcgt aatggcttaa ccgacacagc aatgtatcac
     4021 actgttagcc cggcaggcaa aatatctgcc aggcgtacca gaattacgcg tcgaacgggt
     4081 cgcgcagaat catggtttca gtacgatccg gaccggtaga gatgatatcg atcggcacac
     4141 cggtcagctc ttcaatacgc ttgatgtagt tcagcgccgc ctgcggcagg ccgctacgat
     4201 ctttcacgcc gaaagtggat tcagaccagc ccggcatggt ttcgtaaatc ggctctacac
     4261 ctttccagtc gtcagctgcc agcggagtgg tagtcacttc gcgaccatcc ggcatacggt
     4321 aagccacgca gagtttaacc tctttcaggc catccagaac gtccagttta gtcaggcaga
     4381 agccagacag ggagttcagc tgtaccgcac gacgaacggc aacggtgtcc agccagccgg
     4441 tacgacgacg acgacccgta gttgcaccga attcgttacc ctgcttgcag aggaactcgc
     4501 cagtttcatc aaacagttcg gccgggaaag gacctgcacc tacacgagtt gagtaagctt
     4561 tgaggatacc cagaacgtaa tcaacataac gcgggcccag gccggaaccg gtcgccacgc
     4621 caccagcagt ggtgttggaa gaagttacgt acggataagt accgtggtcg atatccagca
     4681 gcgtaccctg cgcaccttcg aacatgacga aatcgccacg ctgacgcgcc tggtcgagca
     4741 ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc aacagccatc gtatcatcca
     4801 gaactttctg gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg aagttgtgat
     4861 attccatcac ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg tcgccaacac
     4921 gcagaccgcg acgtgccact ttatcttcat aagcaggccc gataccacga ccggtggtgc
     4981 cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc cagagcaacg tgataatcaa
     5041 ggatcagcgg acatgcttca gacagcagca gacgctcacg aacggggatg ccacggtctt
     5101 ccagttcttt catctctttc atcagcgcgg ccggagacag cacaacaccg ttaccgatga
     5161 tgctggttac attctcgcgg agaatacctg atggaataag atggagaacg gttttttcac
     5221 cgttgattac gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca acatatttag
     5281 cccgttcagt cagaagatcg acgatcttac ctttaccttc gtcaccccat tgggtgccca
     5341 gtacgacgac gttgttaccc atttttcaaa atcaccgttt gcttaaaaat ggattctacc
     5401 atcgcttttt cagagttaca gcactttttg cactcaaaaa tgacctcgat cagtctgttt
     5461 tttgttcagc caatcgtttt cctcaacatg tagtagacca caacgcccgc aaccacaagt
     5521 ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga gatcatcttc
     5581 ttccatgcct tcgggtaaag catcggccct aaaccttcca gtaccaaaac cagggcaagc
     5641 gccagccaga ttgtcgaatt cattcattat ccttatagaa aaagaaaacc accgacatcc
     5701 ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt tatattaacg cgttgcggaa
     5761 gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc tcatgaccat cacgtcctga
     5821 ttgccagaga agctgttctc ataagcacgc aggctacgga tgaatgcgta gaagtccgga
     5881 tctttactga atgcatcagc aaacagtttg gctgcttcgg catcgccttc accacgcata
     5941 atgcggccct gacgctcagc ttctgccagc gttctggtca cttcatagtc ggcagtcgcg
     6001 cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac gacgcgctac cgcttcacgc
     6061 tcggcgcgca tacggttgta gatcgcttca gacacttcgg tcggcaggtt gatctgcttg
     6121 atacgcacat cgacaacttc aatacccagc gccgccatac tgttcgggtt gatgaccgga
     6181 actttgccct tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc gttatctgct
     6241 gccggggtag taacttcatc ttctgtaccc gcagaaccgg agttcagcgc gtcacgtact
     6301 tcgagggtca gacgaccacg ggaatcggtg acgatatctt tcacgtccag gcgaccaatt
     6361 tcagaacgca gacggtcaga gaacttacgt ttcaacagca cttccgcttg cgaaatgtcg
     6421 ccaccacccg ttgccaggta gtaacggctg aaatcgctga tgcgccattt gatgtaagag
     6481 tcgacgatca ggtctttctt ctctttggtc acaaagcggt cggcctggtt gtccatggtc
     6541 tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa atgcagacca
     6601 ggctcataaa ccaaaggttt gttgtcatcg tcacgcagta ccttaccaaa acgcagcgta
     6661 ataccgcgct caccttcttt gacgacaaag acagacatgt aaagcactac cagcacgatg
     6721 atgataatcg cgataactga cttacgcatc gttattcccc ctgacgctgg tagtcgttac
     6781 gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg actggtggac gacgtgttgc
     6841 ttgctccact ggttgtggag gaagaggctg gcggcagacg cagcagattg ctggcaccgt
     6901 tatcgctctt cgccgcaggg gcgttaccac ctttcagcat ctggtctaac ggcagaacca
     6961 tcaggttgcc acctttatcg ttaaccagca ctttgcgggt gttacccaac actttttcca
     7021 tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc ggctttatat tccggcagaa
     7081 gtttagcaaa gcgcgccact tcaccctgag cttccaggat ggtctgggcc ttgtacgcac
     7141 gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg cggctgaact tcgttggaat
     7201 acgcttctgc ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc gcatcgtcaa
     7261 acgccgcttt tacttcttcc ggcggacgag cagcctggaa gttgacgtcc agcagcgtga
     7321 tacccatgtc atacggacga atcgtctctt ccagttcgcg ctgagtatcg ctacgaatca
     7381 cggtacgacc ttccgtcaga atgcggtcca tggtgtattt accgataact ccacgcaggg
     7441 cgctgtcggt agcctgacgc aggctgtcat ccgggctggt cacgctatac agatattttt
     7501 ccggattggt gacgcggtac tgcacgttca tctcaacgcg cactacgttc tcgtccgacg
     7561 tcagcatcac accagaagcg gccagttcac gcacggcttc cacgttcacc ggtttgactt
     7621 cgtcgataaa cgtcggtttc cagttcagac ccggctcaac cagatggctg aatttaccaa
     7681 agcgtgttac cacgccgcgt tcggcttctt taatggtata gaaaccactg gccgcccaga
     7741 taatgacaat cgctgccgct gcgatggtaa cgacacgacc gccaagctgc gggcgcgggc
     7801 cttgtgatga actgccaccg ccagatccgg tgcctttacc gccgcccaga ccaccgagct
     7861 ttttgctcag tttgcggaag atatcatcta aatcaggtgg cccttgatcg cgaccgcctt
     7921 tgtttccatt tccctcagag ttgccgccag gtttgctgct tccccacggg tcgcggtctt
     7981 gtccgttatt accgggctga ttccacgcca tgtttgtgct ccatatttgt tatgcggtga
     8041 tccctgttgt ctttcgacgc cagaggatat gactccacgc ttcagacgct acgccgttag
     8101 atcaggtaat cgatcaacgc cggttcttgt ttacagaggc gacgccagtc aacgatcggc
     8161 atacgaactt gcagacttac gctgccgtcc tcctccatcc actctttttc tattgcctga
     8221 agctgataaa aacgactcct cagacgccct tcctgcggtg gcagacgcaa tgtatgctgc
     8281 gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa gctgtggtat ccccgctccg
     8341 gtctgtgcgg aaagccagac acggatcggt ttgttctctt cgtcccgatc aatacgcggt
     8401 tcgaaatctt ccagcatatc gatcttgttc atcaccagca gggttgggat ctcgtgagcg
     8461 tcgatctctt caagaaccgt attcaccgct tcgatgtttt cttgtacacg cacatccgcc
     8521 gcatcaatga cgtgcagcag taatgtggct tgccgcgtct cttgtaacgt ggctttaaat
     8581 gccgccacca gatcgtgcgg caggtggcga ataaacccta cggtatctgc aagtacggtt
     8641 tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt cgagggtggc aaacaactgg
     8701 tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa gggtagattt accggcgttg
     8761 gtatatccca ccagcgaaac agtaggaacg tcggctttga tacgcgattg ccgcccctgc
     8821 tcacgctgct tttcaactct ttccaggcgc gactgtatct gcacgatgcg attacgcaac
     8881 aaacgacggt cggtttcgag ctgggtttca cccggaccac gcaaacctat cccgcctttc
     8941 tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag ccagatggcg cagctgcgcc
     9001 agctcaacct gcaacttacc ctcatgggta cgcgcacgtt gggcgaaaat atctaaaata
     9061 aggccggtgc ggtcgataac acgacactcg cacaaacgct ccaggttacg ctcttgcgcc
     9121 gggctcaggg catggtcaaa aagaacgacc gaagcaccca tcgctttgac agcttccgca
     9181 atttcaactg ctttaccttc acctacaaaa tactttgggt gcggcgcttt acggctaccg
     9241 gtaatcacct gcaatgcttc gacaccggcg gaagagacca gagattcaaa ctcctggagg
     9301 tcttccatat ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg ctcaccagca
     9361 tcataacggt caaacaagcg tataaccctc taaatagatc agcggggaac gcaggatcgc
     9421 tggctccccg tgtaaaaaaa cagcccgaaa ccttattcgg tttcttcgct gtcctgttgc
     9481 gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc gccaccggcg
     9541 ttgttactgt gatgagaaac cgggcgagat gggacaacag tagaaatcgc gtgcttgtaa
     9601 accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga ctcgatttgc
     9661 ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc ccgacgcagt
     9721 gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt tccttatatg
     9781 cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg tctcaattgt
     9841 acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg cctgttctgg
     9901 tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta tctgccgctt
     9961 cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg agatttcgcc
    10021 ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca agtccgtatg
    10081 caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag ccaacatctg
    10141 atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg cgatggcgaa
    10201 ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg ttaaagtttt
    10261 acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat ttggatgaat
    10321 ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact cccaaccttg
    10381 ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg gtagcggcga
    10441 caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca gtgggatccg
    10501 ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat cagcagccga
    10561 gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga gtaactcttc
    10621 agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataaggacag aatcaacgct
    10681 tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt taccggaggc
    10741 cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat cactcatctt
    10801 tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc ggcggcgttt
    10861 tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc tgtgccattg
    10921 accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata ttgccaggtt
    10981 cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt tgtaaatttt
    11041 gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct gactggaaat
    11101 caatacccaa ttccgctagg gcagactgcg ctttttctaa tgccgatttt tcttcgccag
    11161 aaacttttag ccgcaacgga atcagcagcg gctgggcgca aacgggcgct tcacccggcg
    11221 tcaattgtgc ctgacgcagc caacgttctg ccactggcaa ggataaaagt gaaatgttgc
    11281 catcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga ccaaaactct
    11341 gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttggtttt tgcatcggcg
    11401 cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag cctggctgcg
    11461 cattcggcca gggggcagcc ggacgactgc ctgaggcggg cgcaggagag tagcgcggag
    11521 ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc acgcggtttt
    11581 ccgggatggc acgcggtgcg ggttgtggtt catcgtccag cggtagcggc gtttccagtt
    11641 gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga cgcgactgat
    11701 ggaaacgcac ttcgtgtttg gcggggtgta cgttgacgtc cacctgatgc gggtcgatct
    11761 ccagatacaa cacaaatgcc ggttgctgat cggcccccag tttgtcttcg caggcctggc
    11821 ggatcgcatg attgatcagg cgatcgcgca tcatgcgacc attcacgtag caatactgaa
    11881 tttccgccag tgcgggcgta gtgtgatttg gatcggccac ccagccgcgc agggtgagat
    11941 cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa agcggtgccg caaatcgcgc
    12001 ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc acaattttac
    12061 cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg cggatgattt
    12121 catcaatatg gctaaattcg gttttctcgg tgcgcaggaa tttgcgccgc gccggggtgt
    12181 tgtagaacag atccagcacc tccagcgtcg ttcccaccgg atgcgctgcc ggtttaaccg
    12241 tcacgtccat gtcgcgtcct tcggcatagg cctgccaggc ttcctgctgt tctgcggtgc
    12301 gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca ccgcgaaagc
    12361 ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg gcatgacgag
    12421 ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga atgcggataa
    12481 gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca tcgaggctgt
    12541 tttccactag ttctttgact accgacgcag gtcgctcgac cacctcacct gcggcaatct
    12601 ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag tttggcagcg
    12661 tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt tgcaccctgc ggcgcagatt
    12721 gcatcggatg cgcaaggaag taattgcgca ggcctttgta aatggcttct gccagctgtt
    12781 gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata aaaccggttt
    12841 cgaccagtac tgatgggata tccggcgagc gcagaacgcc aaggctggcg tgttctggtc
    12901 gacgtttatg tatttcgcca atgcgttgca actgactgat catactggtc gctacatcat
    12961 accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt aaataggggt
    13021 cagactgact gttcgccagc acatcacccg ccccacccag cagctccgac agtttctcgt
    13081 gctgctccag ccagctggcc atttcactgt tggcgcgacg gttagagagc acccatacgg
    13141 aagcgccagt cgcactacgg ttcggtgcgg catcagcgtg aatcgacacg aggaaattgg
    13201 cgttttgctt atgtgccaca tcactgcgcc ccatcaccga gataaagtaa tccccgtcac
    13261 gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat ttacgcgcga
    13321 tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca gggtcctgac
    13381 cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc gtcgcgcgcg
    13441 ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta ctttccgttt
    13501 taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc gtttcaacgc
    13561 gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta atcgtaaaga
    13621 cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttaccg ttttcggtaa
    13681 gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga atcgccttca
    13741 ccagattatt gccgctgaac aacaacggca gtccctgaat catgcccgtt tgtttgatat
    13801 cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg ccaataaaac
    13861 tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag agcgtcgcgg
    13921 cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg atgcgataca
    13981 tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat tcacccgcag
    14041 aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg tgtatttcga
    14101 catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg cagatggcat
    14161 cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg gcaaggcggt
    14221 acaaatcaaa gtgatagacc attaagttgt cgagcgtata gggttcgacc agcgtataag
    14281 tggggctttt gacattaccc tgatgaccca gagcctgtaa aaagccccgg ctaaaggtgg
    14341 ttttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg caggctttcg
    14401 ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt actcgattca
    14461 tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga aaagagatcg
    14521 gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc cgcaccgtgc
    14581 gcgacacagc ctgcacaggc tgcatcatac ggcgacagtt tttgcccaag caatgcgcca
    14641 ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt tccggcatca
    14701 ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt cagcaccgct
    14761 acgccgccat aacgttgtac cagaggtttg gcgcaatgta agcggtcact ttcaatttca
    14821 gcgacggaac agcctaacaa ccgtgcggcc tcgctaggat gcggcgtgat cacgcgattg
    14881 tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca caacatcggt
    14941 ttgcgaaaat tctcaacttt ttgcagggct tttttccccc actcttgctg gcccagaccg
    15001 ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc catagtcagt
    15061 tcatgcacca tcaattctgg tcgtgcagtc agcagcggcg caatgttctc actgcgggtc
    15121 agtactcgga ctaaaccagc accagcacgt agcgccgctt cccccgtcat acgaatagcc
    15181 cccgccgtgc cgtgatcgcc accgataatc accagccgcc cgtgatcgcc tttatgcgaa
    15241 gtcgggcgac gcggtttcag ccagtgagaa agttgttctg ccgaaaaccg ctgaattttc
    15301 gtctcctgac ctgccagcca gctatccagc cccagtgagt caaaatgcag ttgtcctgta
    15361 acatcccgcg cttttccagt gagcaagcct agtttcagcg caataaaagt gatggtgtga
    15421 tcggcgttga tcactgcgcc tggcgtagcg ccggtttcag ccagcaggcc ggaagggata
    15481 tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg gctaatggat
    15541 tcgcggggcg cttgctgcaa gccggtaccg agcagcgcat caacaatcag atctaccgat
    15601 tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca tgcttcgcgt
    15661 gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggccaacaa cgtgacctca
    15721 atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt attaccatga
    15781 ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac ctggaatgcg
    15841 gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc tgccgcctcg
    15901 cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact tacggggttt
    15961 ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt agcgcaaaaa
    16021 attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga taccgatctc
    16081 agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg cgaaatggac
    16141 tggatggcac gtcacggtat gctgcgcgct cgcccccatg agttattacc cggtacgctg
    16201 cgtgtaatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc cagcacgctg
    16261 aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta tcacaaactt
    16321 ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt ttcgctgaat
    16381 tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga aaaagctggg
    16441 ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc gttcttcttt
    16501 ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga ggaaggatgc
    16561 ggcaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga gccatatacc
    16621 gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc gctcccggaa
    16681 gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca gcttatctgc
    16741 ccgtggaatc gctattcgca actcactaca gaagacgatt tcagcccgcg taagccgcta
    16801 cacgcaccgg aactcattga gttatttgcc tggagcgaag agaagttttt aaaagtcacg
    16861 gaaggatcgg cgattcgccg tataggtcac ctgcgctggc tgcgtaatat cgccgtggca
    16921 ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg taaaggtgag
    16981 cacccacttc tcgatgagca catagcgtgg gcgattgcgc agcaaataga gagacgaaat
    17041 gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt gattgaaaaa
    17101 gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa aacgcattgc
    17161 aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc ttgattttta
    17221 acttatttat caataggtta aaaaaatacc attcaccgtg cgaagtttct ttgaaattaa
    17281 gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag acaacaaaaa
    17341 ccagcaataa cgcatcgcgt cgctgtagat attttattga gagaagaatt t
//
LOCUS       DAZSNO010000078        17081 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZSNO010000078 DAZSNO010000000
VERSION     DAZSNO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17081
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.078"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..510
                     /locus_tag="ACHWN2_002439"
     CDS             <1..510
                     /locus_tag="ACHWN2_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR8931945.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHWN2_002440"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHWN2_002440"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR8931946.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHWN2_002441"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHWN2_002441"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR8931947.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="ACHWN2_002442"
     CDS             complement(2878..3075)
                     /locus_tag="ACHWN2_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR8931948.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHWN2_002443"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHWN2_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR8931949.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHWN2_002444"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHWN2_002444"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR8931950.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHWN2_002445"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHWN2_002445"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR8931951.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHWN2_002446"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHWN2_002446"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR8931952.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHWN2_002447"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHWN2_002447"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR8931953.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHWN2_002448"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHWN2_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="ACHWN2_002449"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="ACHWN2_002449"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR8931954.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHWN2_002450"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHWN2_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR8931955.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="ACHWN2_002451"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="ACHWN2_002451"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR8931956.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="ACHWN2_002452"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="ACHWN2_002452"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR8931957.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="ACHWN2_002453"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="ACHWN2_002453"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR8931958.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHWN2_002454"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHWN2_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
BASE COUNT         3991 a         4864 c         4443 g         3783 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcataggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc cgcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct ggcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt a
//
LOCUS       DAZSNO010000079        16520 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZSNO010000079 DAZSNO010000000
VERSION     DAZSNO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16520
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.079"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1238
                     /locus_tag="ACHWN2_002455"
                     /pseudo
     CDS             1..1238
                     /locus_tag="ACHWN2_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
     gene            1388..1819
                     /gene="ndk"
                     /locus_tag="ACHWN2_002456"
     CDS             1388..1819
                     /gene="ndk"
                     /locus_tag="ACHWN2_002456"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR8931959.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            1969..3123
                     /locus_tag="ACHWN2_002457"
     CDS             1969..3123
                     /locus_tag="ACHWN2_002457"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR8931960.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            3408..4421
                     /gene="rodZ"
                     /locus_tag="ACHWN2_002458"
     CDS             3408..4421
                     /gene="rodZ"
                     /locus_tag="ACHWN2_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR8931961.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            4448..5575
                     /gene="ispG"
                     /locus_tag="ACHWN2_002459"
                     /gene_synonym="gcpE"
     CDS             4448..5575
                     /gene="ispG"
                     /locus_tag="ACHWN2_002459"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR8931962.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            5678..6952
                     /gene="hisS"
                     /locus_tag="ACHWN2_002460"
     CDS             5678..6952
                     /gene="hisS"
                     /locus_tag="ACHWN2_002460"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR8931963.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            6970..7590
                     /gene="yfgM"
                     /locus_tag="ACHWN2_002461"
     CDS             6970..7590
                     /gene="yfgM"
                     /locus_tag="ACHWN2_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR8931964.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            7601..8779
                     /gene="bamB"
                     /locus_tag="ACHWN2_002462"
     CDS             7601..8779
                     /gene="bamB"
                     /locus_tag="ACHWN2_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR8931965.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            8897..10369
                     /gene="der"
                     /locus_tag="ACHWN2_002463"
     CDS             8897..10369
                     /gene="der"
                     /locus_tag="ACHWN2_002463"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR8931966.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            10439..10654
                     /locus_tag="ACHWN2_002464"
     CDS             10439..10654
                     /locus_tag="ACHWN2_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR8931967.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(10651..12021)
                     /gene="xseA"
                     /locus_tag="ACHWN2_002465"
     CDS             complement(10651..12021)
                     /gene="xseA"
                     /locus_tag="ACHWN2_002465"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR8931968.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12183..13649
                     /gene="guaB"
                     /locus_tag="ACHWN2_002466"
     CDS             12183..13649
                     /gene="guaB"
                     /locus_tag="ACHWN2_002466"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR8931969.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            13718..15295
                     /gene="guaA"
                     /locus_tag="ACHWN2_002467"
     CDS             13718..15295
                     /gene="guaA"
                     /locus_tag="ACHWN2_002467"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR8931970.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(15390..15929)
                     /locus_tag="ACHWN2_002468"
     CDS             complement(15390..15929)
                     /locus_tag="ACHWN2_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR8931971.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(15945..16463)
                     /locus_tag="ACHWN2_002469"
     CDS             complement(15945..16463)
                     /locus_tag="ACHWN2_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR8931972.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT         3846 a         4100 c         4568 g         4006 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgtc gccccactgg catggaaaac gggcaccagc tatggctatc
      481 gtgacgcctg ggcgattggg gttaacgctc gctatgtcat tgggatctgg actggcagac
      541 ctgacggcac gcccgttgtt ggtcagtttg gctttgccag tgccgtacca ttgttgaatc
      601 aggtcaataa tatcttactg tcgcgcagtg cgaatctgcc ggaagacccg cgcccgaact
      661 cagtcactcg tggcgttatc tgctggccgg gcggacagtc tttgccggaa ggtgacggta
      721 actgtcgccg ccgcctggca acctggctgc tggatggcag ccagccgcca acgctgttac
      781 tgtcagagca ggaagggatt aatggcattc gcttccccat ctggctggat gacaaagggc
      841 agcgcgtcgc cgctgactgc ccgcaggcga agcaagaaat ggtaaatgtc tggccgctac
      901 cgctggaacc ctggctgccc gcatcagaac gtcgcgctgt gcgcttacca ccggcctcga
      961 caatttgccc gccatacggt cacgatgccc agctccctct gcagctgaca ggcgttcgcg
     1021 atggcgcgat tattaaacgt ttaccgggcg cagcggaagc gactttgccg ttgcaatcaa
     1081 gtggaggggc aggtgaacgc tggtggtttc tgaatggcga accgttaact gaacgcgggc
     1141 gcaacgtgac cctgcatttg acggataaag gtgattatca attgctggtg atggatgatg
     1201 tggggcaaat cgcgacagtg aaatttgtca tgcaatagtc tgttttgtcc ctgattgttg
     1261 ctaaaactca ttttatttta aaaaaaatgt taccttcatc aatagtcaac ggccctgttg
     1321 ctcattataa tccgcgccat ctcgtacgct ggtacagaca acaacagaac aatttacaga
     1381 ggtaaaaatg gctattgaac gtactttttc catcatcaaa ccgaacgcgg tagcaaaaaa
     1441 cgtcattggt aatatctttg cgcgctttga agctgcaggg ttcaaaattg tcggcaccaa
     1501 aatgctgcat ctgaccgttg aacaggcacg tggcttttat gctgaacacg atggaaaacc
     1561 gttctttgat ggtctggttg aattcatgac ctctggcccg atcgtggttt ccgtgctgga
     1621 aggtgaaaac gccgttcagc gtcaccgcga tctgctgggc gcgaccaatc cggcaaacgc
     1681 actggctggt actctgcgcg ctgattacgc tgacagcctg accgaaaacg gtacccacgg
     1741 ttctgattcc gtcgaatctg ccgctcgcga aatcgcttat ttcttcggcg aaggcgaagt
     1801 gtgcccgcgc acccgttaat aatttcgtaa atgccgcgtg caaacgtggc atccgtgcgc
     1861 cagaatttgt acaatgcagc gcccccggac gagcagccgc tcaccggggc gtttcttttt
     1921 tcaaccctcc aggggccata acgtgtaata acgaggccgg aatagcatat gtctgaacaa
     1981 ttagtcacac ctgaaaacgt caccacgaaa gatggaaaaa tcaacctgct ggatctcaac
     2041 cgtcagcaga tgcgggagtt ttttaaagat ttaggtgaaa aaaccttccg cgccgatcag
     2101 gtgatgaagt ggatgtatca ctattgctgc gacaactttg atgagatgac cgacatcaac
     2161 aaagtgttgc gcggcaaact gaaagaggtg gcggaaatcc gcgcaccgga agtggttgaa
     2221 gaacagcgtt catccgacgg caccattaaa tgggcgatcg ccgttggcga tcagcgcgtc
     2281 gaaacggtgt atatcccgga agacgaccgt gccacgctct gcgtctcttc gcaggtgggg
     2341 tgtgcgctgg agtgtaaatt ctgttccacc gcccagcagg gctttaaccg caacctgcgg
     2401 gtatcggaaa ttattggcca ggtgtggcgt gcggcgaaaa tcgtcggcgc agcaaaagtc
     2461 accggtcagc gcccaatcac taacgtcgtg atgatgggca tgggcgagcc gctgctcaac
     2521 ctgaacaacg tcgttccggc gatggaaatc atgcttgatg atttcggttt tggcctgtct
     2581 aaacgtcgcg tgacgctttc cacttccggt gtagttccgg cgctggataa actgggcgat
     2641 atgatcgacg ttgcactggc aatttccctg cacgcgccga acgacgaaat tcgtgacgaa
     2701 attgtgccga tcaacaaaaa gtacaatatc gaaacgttcc ttgctgcggt gcgccgttat
     2761 ctggagaaat ccaacgccaa tcagggccga gtcactattg aatacgtgat gcttgaccac
     2821 gttaacgacg gcactgaaca cgcgcaccaa ctggcggaac tgctgaaaga tacgccgtgt
     2881 aagatcaacc tgatcccgtg gaacccgttc ccggacgcgc cgtatggacg cagttcgaac
     2941 agccgtatcg accgtttctc aaaagtgctg atgagctacg gtttcaccac tattgtgcgt
     3001 aaaacgcgtg gtgatgatat cgatgctgcc tgtggtcagt tggcgggcga tgttatcgac
     3061 cgtacgaaac gtactctgcg taaacggatg cagggtgaag ccatcgatat taaagcggtc
     3121 tgatagccat tacgccacgg ttactttatg taccgtggcg taatctttta tgaatgaagt
     3181 attaagttgc aattgtctgg tcaatattaa cggtgcgttt ttgctgactt taaggcagta
     3241 tgtaacgatg caacagtaag ttagcctcag tgaatgtggg ctttgtcacg agcacacaga
     3301 cggtcttata ctgtatgata acggttaact taacggatgt ttcgcggtgt gggtgagcat
     3361 gatggttcac cggcatctca attctcattt aaacgtacct gcagcgaatg aatactgaag
     3421 ccacgcacga ccaaaatgaa gcacttacta ccggcgctcg cctgcgtaat gctcgcgaac
     3481 aactaggact tagtcagcag gccgttgccg agcgactttg cctgaaggtt tccacggtac
     3541 gcgacattga agaagataag gcacccgccg atcttgcttc aacattcctg cgcggatata
     3601 tccgctctta tgcgcgtctg gtacatattc cagaagaaga actgctgcca gggctggaaa
     3661 agcaggctcc acttcgggct gcaaaagttg cgccaatgca gagtttttcc ctcggtaaac
     3721 gccgcaaaaa acgcgacggc tggctaatga ccttcacctg gctggtgttg tttgtggtta
     3781 tcggcctgag cggtgcctgg tggtggcaag accacaaagc tcagcaggaa gagatcacca
     3841 ctatggccga tcaatcttcg gcggaactga gcagtaatag cgagcagggg cagagtgttc
     3901 cgttaaatac gtcgacaact acagacccgg ctacaacgtc cacgccgcca gcgtctgtgg
     3961 atactaccgc aaccaacacg caaacacctg ccgtaactgc gccagcacca gctgttgatc
     4021 cgcaacagaa tgcggttgtt tcgccttcgc aggcaaatgt tgataccgcc gcgaccccgg
     4081 taccaacggc agcaacaacg ccagatggtg ctgcgccgtt gccaaccgat caggctggcg
     4141 tgaccacgcc ggcggctgat ccgaatgcgc tggtgatgaa ctttactgcc gattgctggc
     4201 tggaggtcac tgatgctacc ggtaaaaaat tgtttagcgg gatgcagcgt aaagacggta
     4261 atttgaattt aaccggccag gcaccgtata aactgaaaat tggtgcgcca gccgcagtac
     4321 agatccagta tcaagggaaa cctgtcgatc tgagtcgttt tatcagaact aaccaggttg
     4381 cgcgtctgac cctcaatgcc gaacaatcac cggcgcagta acagacgggt aacgcgggag
     4441 atttttcatg cataaccagg ctccaattca acgtagaaaa tcaacacgta tttacgttgg
     4501 gaatgtgccg attggcgatg gtgctcccat cgccgtacag tccatgacca atacgcgtac
     4561 gacagatgtc gaagcaacgg tcaatcaaat caaggcgctg gaacgcgttg gcgctgatat
     4621 cgtccgtgtc tccgtaccga cgatggacgc ggcagaagcg ttcaaactca tcaaacagcg
     4681 ggttaacgtg ccgctggtgg ctgacatcca cttcgactat cgcattgcgc tgaaagtagc
     4741 ggaatacggc gtcgattgtc tgcgtattaa ccctggtaat atcggtaatg aagagcgtat
     4801 tcgcatggtg gttgactgtg cgcgcgataa aaacattccg atccgtattg gcgttaacgc
     4861 cggatcgctg gaaaaagatc tgcaagaaaa gtatggcgaa ccgacgccgc aggcgttgct
     4921 ggaatccgcc atgcgtcatg ttgatcatct cgatcgcctg aacttcgatc agttcaaagt
     4981 cagcgtgaaa gcgtctgacg tcttcctcgc tgttgagtct tatcgtttgc tggcaaaaca
     5041 gatcgatcag ccgctgcatc tggggatcac cgaagccggt ggtgcacgca gcggggcagt
     5101 aaaatccgcc attggtttag gtctgctgct gtcggaaggc atcggcgaca cgctgcgtgt
     5161 atcgctggcg gccgatccgg tcgaagagat caaagtcggt ttcgatattt tgaaatcgct
     5221 gcgtatccgt tcgcgaggga ttaacttcat cgcctgcccg acctgttcgc gtcaggaatt
     5281 tgatgttatc ggtacggtta acgcgctgga gcaacgcctg gaagatatca tcactccgat
     5341 ggacgtttcg attatcggct gcgtggtgaa tggcccaggt gaggcgctgg tttctacact
     5401 cggcgtcacc ggcggcaaca agaaaagcgg cctctatgaa gatggcgtgc gcaaagaccg
     5461 tctggacaac aacgatatga tcgaccagct ggaagcacgc attcgtgcga aagccagtca
     5521 gctggacgaa gcgcgtcgaa ttgacgttca gcaggttgga aaaataataa cgtgatggga
     5581 agcgcctcgc ttcccgtgta tgattgaacc cgcatggctc ccgaaacatt gagggaagcg
     5641 ttgagggttc atttttatat tcagaaagag aataaacgtg gcaaaaaaca ttcaagccat
     5701 tcgcggcatg aacgattacc tgcctggcga aacggccatc tggcagcgca ttgaaggcac
     5761 actgaaaaac gtgctcggca gctacggtta cagtgaaatc cgcttgccga ttgtagagca
     5821 gaccccgcta ttcaaacgtg cgattggtga agtcaccgac gtggttgaaa aagagatgta
     5881 cacctttgag gatcgcaatg gcgacagcct gactctgcgc cctgaaggga cggcgggctg
     5941 tgtacgcgcc ggcatcgagc atggtcttct gtacaatcag gaacagcgtc tgtggtatat
     6001 cgggccgatg ttccgtcacg agcgtccgca gaaagggcgt tatcgtcagt tccatcagtt
     6061 gggctgcgaa gttttcggtc tgcaaggtcc ggatatcgac gctgaactga ttatgctcac
     6121 tgcccgctgg tggcgtgcgc tgggtatctt cgaacacgta actcttgagc tgaattctat
     6181 cggttcgctg gaagcacgcg ccaattaccg cgatgcgctg gtggcattcc ttgagcagca
     6241 taaagaaaag ctggacgaag actgcaaacg ccgcatgtac actaacccgc tgcgcgtgct
     6301 ggattcaaaa aatccggaag tgcaggcgct tctcaacgac gctccggcat taggtgatta
     6361 tctggacgag gaatctcgtg agcattttgc cggtctgtgc aaactgctgg agagcgcggg
     6421 gatcgcttac accgtaaacc agcgtctggt gcgtggtctg gattactata accgtaccgt
     6481 tttcgagtgg gtgactaaca gtctcggctc ccagggcacc gtgtgtgcag gcggtcgtta
     6541 tgacggtctt gtggaacaac tgggcggtcg tgcaacaccg gctgtcggtt ttgcgatggg
     6601 cctcgaacgt cttgtattgt tagtacaggc cgttaatccg gaatttaaag ccgatcctgt
     6661 tgtcgatata tacctggtgg cttcaggtgc tgatacacaa tctgcggcta tggcattagc
     6721 tgagcgtctg cgtgatgaat tactgggcgt gaaattgatg accaaccacg gcggcggcaa
     6781 ctttaagaaa cagtttgccc gtgctgataa atggggtgcc cgcgttgctg tggtgctggg
     6841 tgagtctgaa gtggctaacg gcacagcagt agtgaaggat ttgcgctctg gtgagcaaac
     6901 ggcagttgcg caggatagcg tagccgcgca tttgcgcacg ttactgggtt aaggaaggag
     6961 aaggacagcg tggaaattta cgagaacgaa aacgaccagg tagaagcggt taaacgcttt
     7021 tttgctgaaa atggcaaagc actggctgtt ggggggattt tgggcgttgg cgcactgact
     7081 ggctggcgct actggaacag ccatcaggtt gattctgcac gctccgcttc tcttgcctat
     7141 caaaatgcgg ttaccgcagt gagcgaaggc aaaccggata gcatcccggc ggcggaaaaa
     7201 tttgctgctg aaaataaaaa tacttatggt gcgctggctt ctttggaact tgcgcagcaa
     7261 tttgttgaca aaaatgaact agagaaagct gccgcccagt tacaacaggg gctggcagac
     7321 acgagcgatg aaaatctcaa agccgtgata aatctgcgtc ttgctcgcgt tcaggtacag
     7381 ctcaagcagg ctgatgccgc gctgaaaacc cttgatacca tcaaaggtga agggtgggct
     7441 gccattgttg ccgacttgcg tggtgaagca ttgctgagca aaggtgataa gcaaggtgcg
     7501 cgtagtgcat gggaagcagg cgtgaaaagc gatgttactc cggcactgag cgaaatgatg
     7561 cagatgaaaa ttaataattt gtccatctga gagggacccg atgcaattgc gtaaattact
     7621 gctgccagga ctgctttccg ttaccctttt aagcggctgt tcgctgttta acagcgaaga
     7681 agatgtggta aagatgtccc cattgccaac cgttgaaaac cagtttacgc cgaccacggc
     7741 gtggagcact tccgttggta gcggcattgg caacttctat tccaatcttc atccggcact
     7801 ggcggacaac gttgtctatg cagcggaccg cgctggtttg gtaaaagcgc tgaatgcgga
     7861 tgatggcaaa gaaatctggt ctgtcagcct ggccgagaaa gatggctggt tctctaaaga
     7921 gcctgcatta ctttctggcg gtgtgaccgt gtctggtggg catgtctaca ttggcagcga
     7981 aaaggcgcag gtttacgcgc tgaataccag cgatggtact gtggcatggc aaactaaagt
     8041 cgcgggtgaa gcactttcgc gcccggtggt cagcgacggt ctggtgttaa tccacaccag
     8101 taacggtcag ttacaagcgc tgaacgaagc tgacggcgct gtcaaatgga cagttaacct
     8161 cgatatgcct tcgctctctt tgcgtggcga gtctgcgccg gcaacggctt ttggtgcggc
     8221 cgtcgtgggt ggcgataatg gtcgcgtcag cgcagtgctg atggaacagg gccagatgat
     8281 ttggcagcag cgtatttccc aggcgaccgg ttctaccgaa attgaccgtc tgagcgatgt
     8341 tgacacgact cccgtcgttg ttaacggcgt tgttttcgcg ctggcctata atggtaacct
     8401 gacggcgctt gatctgcgca gtggtcagat tatgtggaaa cgcgaactgg gttcggtgaa
     8461 tgatttcatc gtcgatggca atcgcatcta tctggtcgat caaaatgacc gggtgatggc
     8521 gttgaccatt gatggcggcg ttacgctgtg ggcacaaagc gatctgctgc atcgcctgct
     8581 gacttctccg gtgctgtata atggcaacct ggtggtcggt gacagtgaag gttatctgca
     8641 ctggattaac gtcgaagatg gtcgtttcgt tgcccagcaa aaagttgata gttccggttt
     8701 ccagactgaa ccggttgccg ctgacggcaa actgctgatc caggcaaaag acggaaccgt
     8761 gtactctatt acacgttaat cgtctctgtc gttcactttg aaaacggctc ctggacaggg
     8821 gccgttttcc tgtttttaac aacgacgcga atatagcgtg cgttgtctga tgatttataa
     8881 aaatgaggct ttaaacatgg tacctgtggt cgcgcttgtc gggcgcccta acgtaggaaa
     8941 atccacgtta tttaaccgtc taactcgcac ccgagatgcg ctggttgcgg atttcccggg
     9001 tctgactcgt gaccgtaagt acggtcgtgc ggaaattgaa ggccgtgagt ttatctgtat
     9061 tgataccggc gggattgatg gcacagaaga cggtgtagaa acccgcatgg cggaacagtc
     9121 gctgctggcg attgaagaag cggacgtcgt actgtttatg gtggatgcgc gcgcgggcct
     9181 gatgccggca gatgaagcga ttgccaaaca tctgcgctcc cgtgaaaaac cgaccttcct
     9241 ggtggtaaac aaaactgacg gtctggatcc cgatcaggca gtggttgatt tctactcgct
     9301 tggtttaggt gaaatctacc cgatcgccgc gtctcacggt cgtggcgtat taagtctgct
     9361 ggagcatgtg ctgctgccgt ggatggaaga tctcgcaccg caagaggaag tcgacgaaga
     9421 cgctgaatac tgggcgcaat ttgaagcgga agagaacggc gaagaagaag aggaagacga
     9481 cttcgacccg caaagtctgc cgatcaaact ggcgattgtg ggtcgtccga acgtaggtaa
     9541 gtctacactc actaaccgta ttcttggaga agagcgcgtt gttgtttacg acatgcctgg
     9601 cacgacgcgt gacagcatct acatcccaat ggaacgcgat ggacgtgagt atgtgctcat
     9661 tgacaccgct ggcgtacgta aacgcggcaa aatcaccgat gctgtagaga aattctccgt
     9721 aatcaaaacg ttgcaggcca ttgaagacgc caacgtggtg atgttagtga ttgatgcgcg
     9781 cgaaggtatt tccgatcagg atctctcgct gctgggcttt attctcaata gtgggcgctc
     9841 acttgtcatt gtggtgaata agtgggatgg cctgagccag gaagtgaaag agcaggtgaa
     9901 agaaacgctg gacttccgtc tgggctttat cgattttgct cgtgtgcact ttatctctgc
     9961 cttgcacggc agtggtgttg gtaacttgtt tgaatcagta cgtgaagcgt atgacagctc
    10021 cacccgtcgt gtggggacct ctatgctgac gcgcatcatg acgatggctg ttgaagatca
    10081 ccaaccgccg ctggtacgcg gtcgtcgtgt gaagctgaaa tatgcccacg ctggtggtta
    10141 taacccgccg attgtggtga ttcacggtaa tcaggtgaaa gacctgcctg attcctacaa
    10201 gcgctacttg atgaactact tccgcaaatc gctggacgta atgggatcgc cgattcgtat
    10261 tcagttcaaa gaaggggaaa acccgtatgc gaacaagcgt aacaccctga cgccaaccca
    10321 gatgcgtaaa cgtaagcgtc tgatgaagca catcaagaaa aataaataat tatccgtaag
    10381 tattttatct gccattccct ctatgaatgt agagggaatg gctaccggag gaattcatat
    10441 ggaactgcat tgtccaaaat gtcagcatgt gcttgatcag gataacggcc atgcgcgttg
    10501 cccatcctgc ggggaattta ttgaaatgaa agctctttgt ccagactgcc atcaaccgtt
    10561 gcaggtgtta aaagcctgcg gtgcagtaga ttacttctgt cagcacgggc atggcttgat
    10621 atcgaaaaaa cgcgttgaat tcgtgctggc ttaatgaacc tttttacgcg atttttttac
    10681 cggctggatg ttttttactt cactttctat ccagccgtct tccagacgtg tggttagcat
    10741 ttcacccgtt ttaacttgct tcactttttt cagtacattg ccgtcagtag cagtagtaac
    10801 gctgtatcca cgcgccagcg ttgacagtgg gcttacggct tcgaggtgcg ttactgcatt
    10861 accgaaacgt tcacgcgtgg cgctaagctg tgcgcgcagg atttctgcta aacgatattc
    10921 cagttgctga atgcgcgttt gcgtgcgatg aatcttcggc tgtggatttt gctgattcag
    10981 ccgctgtgtt aatcgctgct gctgttgccc ggcacgctta agctgatttt ccagcgcaaa
    11041 gctcatccgt ttttgcaggc gttcaagcat ggtttgctgg cgtgccagcc ggagctgcgg
    11101 atgctgctgc tgcaagcgat gatgaatctg cgtaaagcga cgtgtacggt tggcgagata
    11161 ataatccatc gccatctcca gccgttgacg ggccgattgc acctggcgca gtaactcttg
    11221 ctgattacgg ctcactactt cagcggcggc agacggcgtt ggcgcacgca gatcggcaac
    11281 aaaatcggca atggtcacat ccgtctcatg cccgacggcg ctgacgaccg gaatgcggct
    11341 ggcaaaaatc gcccgcgcta cgcgttcgtc gttaaaactc cataaatctt ccagcgaacc
    11401 gccgccgcgc ccaacgatca acacgtcgca ctcattgcgc tgattcgcca gttcaatggc
    11461 gcgaacaatt tgccccggcg cgtcatcgcc ctgaacggag gtggggtaga tgatcaccgg
    11521 tagagaagga tcgcgacgtt ttaacacatg caaaatatca tgtagcgcag caccggtttt
    11581 tgaggtgatc acaccaacgc aatgcgcagg ggagggaagt ggttttttgt attgctgatc
    11641 gaacaaacat tcagcctgca acttcgcttt gagctgttcg tacttctgtt gcagcagccc
    11701 ttcaccggcc ggctgcatac tctcaacgat tatctggtag tcgccgcgcg gctcgtagag
    11761 cgtaatattg gcgcgaacta aaacttgttg cccatgctgc ggacggaagg tgacccggcg
    11821 gttgctgttg cggaacatcg cgcagcgtac ctgggcggtg tcgtctttga gtgtaaagta
    11881 ccagtgaccg gaagccggtt gtgtgaaatt agagatttcg ccgctgatcc aaacctgtcc
    11941 catctcatgc tcaagcagca gacgaaccgt ttgattcagg cgactaacgg taaaaattgc
    12001 aggggattga gaaggtaaca tgtgagcgag atcaaattct aaatcagcag gttattcagt
    12061 ctatagtaac ccgcccttcg gggatagcaa gcattttttg caaaaagggg tagatgcaat
    12121 cggttacgct ctgtataatg ccgcggcaat atttattaac cactctggtc gagatattgc
    12181 ccatgctacg tatcgctaaa gaagctctga cgtttgacga cgttctcctc gttcctgctc
    12241 attctaccgt tctgccgaat actgctgacc tcagcaccca gctgacgaaa actattcgtc
    12301 tgaatatccc tatgctttcc gcagcaatgg ataccgtaac ggaagcgcgc ctggctattg
    12361 ctctggctca ggaaggcggt atcggcttta tccacaaaaa catgtccatt gaacgccagg
    12421 cagaagaagt tcgccgtgtg aaaaaacacg aatctggtgt ggtgactgat ccgcagactg
    12481 ttctgccaac cacgacgctg cgcgaagtga aagaactgac cgagcgtaac ggttttgcgg
    12541 gctatccggt cgttaccgaa gaaaacgaac tggtgggtat tatcaccggt cgtgacgtgc
    12601 gttttgttac cgacctgaac cagccggtta gcgtttacat gacgccgaaa gagcgtctgg
    12661 tcaccgtgcg tgaaggtgaa gcccgtgaag tggtgctggc aaaaatgcac gaaaaacgcg
    12721 ttgaaaaagc gctggtggtt gatgacgaat tccacctgat cggcatgatc accgtgaaag
    12781 acttccagaa agcggaacgt aaaccgaacg cttgtaaaga cgagcaaggc cgtctgcgtg
    12841 ttggtgctgc agttggcgca ggtgcgggta acgaagagcg tgttgacgcg ctggttgccg
    12901 ctggcgttga cgttctgctg atcgactcct cccacggtca ctcagaaggc gttttgcagc
    12961 gtatccgtga aacccgtgct aaatatccgg atctgcaaat catcggcggc aacgtggcaa
    13021 cagctgcagg tgcacgcgct ttggcagaag ctggttgcag cgcggttaaa gtcggtatcg
    13081 gccctggttc tatctgtacg actcgtatcg ttactggcgt aggtgttccg cagatcactg
    13141 ccgttgctga cgcagttgaa gccctggaag gcaccggtat tccggttatc gcagatggtg
    13201 gtattcgctt ctccggcgac atcgccaaag ctatcgccgc tggcgcaagc gcggtgatgg
    13261 taggttccat gctggcgggt actgaagaat ctccgggtga aatcgaactc taccagggcc
    13321 gttcttataa atcttaccgt ggtatgggtt ccctgggcgc gatgtccaaa ggttcctctg
    13381 accgttattt ccagagcgat aacgctgccg acaaactggt gccggaaggt atcgaaggtc
    13441 gcgtagctta taaaggtcgc ctgaaagaga tcattcacca gcagatgggc ggcctgcgct
    13501 cctgtatggg tctgaccggc tgtggtacta tcgacgaact gcgtactaaa gcggagtttg
    13561 tacgtatcag cggtgcgggc attcaggaaa gccacgttca cgacgtgacc attactaaag
    13621 agtccccgaa ctaccgtctg ggctcctgat tctcttcgcc cgatttcatg tcgggcgatt
    13681 tatattatct gtttcacttg cctcggaata agcgtcaatg acggaaaaca ttcataagca
    13741 tcgcatcctc attctggact tcggttctca gtacactcaa ctggttgcgc gccgcgtgcg
    13801 tgagctgggt gtttactgcg aactgtgggc gtgggatgtg acagaagcac aaattcgtga
    13861 cttcaatcca agcggaatta ttctttccgg cggcccggaa agtactactg aagaaaacag
    13921 tccgcgtgcg ccgcagtatg tctttgaagc aggcgtaccg atattcggcg tttgctatgg
    13981 catgcagacc atggcaatgc agttgggcgg tcacgttgaa gcctctaacg aacgtgaatt
    14041 tggctacgcg caggtagaag tcgtaaacga cagcgcactg gttcgcggta tcgaagatgc
    14101 gctgaccgca gacggtaaac cgctgctcga tgtctggatg agccacggcg ataaagttac
    14161 cgctatcccg tccgacttcg tcaccgtagc cagcaccgaa agctgcccgt ttgccattat
    14221 ggctaacgaa gaaaaacgct tctatggcgt acagttccac ccggaagtga ctcacacccg
    14281 ccagggtatg cgcatgctgg agcgttttgt gcgtgatatc tgccagtgtg aagccctgtg
    14341 gacgccagcg aaaattatcg acgatgctgt agctcgcatc cgcgagcagg taggcgacga
    14401 taaagtcatc ctcggcctct ctggtggtgt ggattcctcc gtaaccgcaa tgctgctgca
    14461 ccgtgctatc ggtaaaaacc tgacttgcgt attcgtcgac aacggcctgc tgcgtctcaa
    14521 cgaagcagag caggttctgg atatgtttgg cgatcacttt ggtcttaaca ttgttcacgt
    14581 accggcagaa gatcgcttcc tgtcagcgct ggctggcgaa aacgatccgg aagcaaaacg
    14641 taaaatcatc gggcgcgttt tcgttgaagt attcgatgaa gaagcgctga aactggaaga
    14701 cgtgaagtgg ctggcgcagg gcaccatcta ccctgacgtt atcgaatctg cggcttctgc
    14761 aaccggtaaa gcacacgtca tcaaatctca ccacaacgtg ggcggcctgc cgaaagagat
    14821 gaagatgggc ctggttgaac cgctgaaaga gctgttcaaa gacgaagtgc gtaagattgg
    14881 tctggagctg ggcctgccgt acgacatgct gtaccgtcat ccgttcccgg gaccaggcct
    14941 tggcgttcgt gttctgggtg aagtgaagaa agagtactgt gacctgctgc gccgtgctga
    15001 cgccatcttc attgaagaac tgcgtaaagc ggacctgtac gacaaagtca gccaggcgtt
    15061 caccgtcttc ctgccagtac gttccgttgg cgtaatgggc gatggtcgta agtatgactg
    15121 ggttgtctct ctgcgtgctg tcgaaaccat cgactttatg accgcacact gggcgcatct
    15181 gccgtacgat ttcctcggtc gcgtttccaa ccgcattatc aatgaagtga acggtatttc
    15241 ccgcgtggtg tatgacatca gcggcaagcc gccagctacc attgagtggg aatgattttg
    15301 atccctgcac tatgaatgaa caaaaccctc cgttactaca gagggttttt tatcttcaag
    15361 aatcataggc ttgaagttac taacatcgat taattaaacc agctgtccga tttgttctct
    15421 tctgctttgc ccacgctttt catcagatcg cgaccgcctt cagtcatatt tctgtttgcg
    15481 tcagcttcag attgcaccac atcggtttgc gcagctttgt gcttcagttc ctgatcgata
    15541 aattcgtttt cgcgcttaac gcgggcttct tctttcgcca gcgccagttt ttgtttctga
    15601 atctctaagc tgcgtagctc atcttcataa ctttgatcgc gttttttgtc cgcagtggct
    15661 tcggcgtcca gtttatcctg acgagctttc ttatttgccg ctgccgttgc cgctcttttg
    15721 ttcgcggcgg cctgggcatt tgcgcgacgt tgcttctctt gctggatttc cctgttgcgc
    15781 tccgcgaccc attcgtcatg ctgcctttgc tcttcatttt taccttgctg ttccgcttct
    15841 gcgacagcag agagttgatc ctgcaatgat gaggctatag ccggatagct taaggaggct
    15901 aagatggcgc aaagaaaaac tttcttcatg actcctcctg attattagct cttttcagga
    15961 catttggtat ttggctgaat acgcgtttcg ttatacgttg tggtaataac aacggctaaa
    16021 cctgtcgtaa actggcactc tttaccctcc tgggtggagg tatacacttt ggtgccttcc
    16081 ttatatgtta aagaaacacc ttccactaag gttttatcat tcaccataga acccgctgcc
    16141 gcgcctacag ctccgccgcc aactgcccct gccgtcgttc cggaattgct gccagaaccg
    16201 acgttgtggc cgataacacc gccagcgact gcgccaataa gcgcgccgaa ggcttgtgcg
    16261 ttccgtttat tttgggagtt gtctacggca acttttgcgg gaagaatgga aataatatta
    16321 acggttttag tttcttgttt ggtattcagt tgatcggttt gataaacatc ggcggcatga
    16381 tcgtcagcat ttgactggca tcctgccaga gtgaatgacg ctaacattgc cacaggcaga
    16441 agacattttt taaatttcat cactattcct tgttatacac attacgaaaa ataagacatt
    16501 taagatcatc aaaagaggaa
//
LOCUS       DAZSNO010000080        16362 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZSNO010000080 DAZSNO010000000
VERSION     DAZSNO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16362
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.080"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHWN2_002470"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHWN2_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHWN2_002471"
     CDS             complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHWN2_002471"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR8931973.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHWN2_002472"
     CDS             complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHWN2_002472"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR8931974.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHWN2_002473"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHWN2_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="ACHWN2_002474"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="ACHWN2_002474"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR8931975.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="ACHWN2_002475"
     CDS             complement(5877..6218)
                     /locus_tag="ACHWN2_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR8931976.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHWN2_002476"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHWN2_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR8931977.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHWN2_002477"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHWN2_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR8931978.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="ACHWN2_002478"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="ACHWN2_002478"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR8931979.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="ACHWN2_002479"
     CDS             12897..14240
                     /locus_tag="ACHWN2_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR8931980.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="ACHWN2_002480"
     CDS             complement(14302..14508)
                     /locus_tag="ACHWN2_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR8931981.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="ACHWN2_002481"
     CDS             14833..15387
                     /locus_tag="ACHWN2_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR8931982.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..>16362)
                     /locus_tag="ACHWN2_002482"
     CDS             complement(15450..>16362)
                     /locus_tag="ACHWN2_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8931983.1"
                     /translation="FIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWVSNYDSLLTL
                     RESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLRGSGFEYEEI
                     PITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQAFGDVSFGY
                     ALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGD
                     ERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICE
                     KYKSTIEAFKKEQLALPVLVRCKNSET"
BASE COUNT         3919 a         4451 c         3985 g         4007 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aattgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aa
//
LOCUS       DAZSNO010000081        16096 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZSNO010000081 DAZSNO010000000
VERSION     DAZSNO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.081"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..588
                     /locus_tag="ACHWN2_002483"
     CDS             <1..588
                     /locus_tag="ACHWN2_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFR8931984.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="ACHWN2_002484"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="ACHWN2_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFR8931985.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="ACHWN2_002485"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="ACHWN2_002485"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFR8931986.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="ACHWN2_002486"
                     /pseudo
     CDS             complement(1511..1978)
                     /locus_tag="ACHWN2_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
     gene            2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACHWN2_002487"
     CDS             2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACHWN2_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR8931987.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2701..3330
                     /gene="rplC"
                     /locus_tag="ACHWN2_002488"
     CDS             2701..3330
                     /gene="rplC"
                     /locus_tag="ACHWN2_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR8931988.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3341..3946
                     /gene="rplD"
                     /locus_tag="ACHWN2_002489"
     CDS             3341..3946
                     /gene="rplD"
                     /locus_tag="ACHWN2_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR8931989.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3943..4245
                     /gene="rplW"
                     /locus_tag="ACHWN2_002490"
     CDS             3943..4245
                     /gene="rplW"
                     /locus_tag="ACHWN2_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR8931990.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4263..5084
                     /gene="rplB"
                     /locus_tag="ACHWN2_002491"
     CDS             4263..5084
                     /gene="rplB"
                     /locus_tag="ACHWN2_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR8931991.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5101..5379
                     /gene="rpsS"
                     /locus_tag="ACHWN2_002492"
     CDS             5101..5379
                     /gene="rpsS"
                     /locus_tag="ACHWN2_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR8931992.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5394..5726
                     /gene="rplV"
                     /locus_tag="ACHWN2_002493"
     CDS             5394..5726
                     /gene="rplV"
                     /locus_tag="ACHWN2_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR8931993.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5744..6445
                     /gene="rpsC"
                     /locus_tag="ACHWN2_002494"
     CDS             5744..6445
                     /gene="rpsC"
                     /locus_tag="ACHWN2_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR8931994.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6458..6868
                     /gene="rplP"
                     /locus_tag="ACHWN2_002495"
     CDS             6458..6868
                     /gene="rplP"
                     /locus_tag="ACHWN2_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR8931995.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6868..7059
                     /gene="rpmC"
                     /locus_tag="ACHWN2_002496"
     CDS             6868..7059
                     /gene="rpmC"
                     /locus_tag="ACHWN2_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR8931996.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACHWN2_002497"
     CDS             7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACHWN2_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR8931997.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7478..7849
                     /gene="rplN"
                     /locus_tag="ACHWN2_002498"
     CDS             7478..7849
                     /gene="rplN"
                     /locus_tag="ACHWN2_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR8931998.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7860..8174
                     /gene="rplX"
                     /locus_tag="ACHWN2_002499"
     CDS             7860..8174
                     /gene="rplX"
                     /locus_tag="ACHWN2_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR8931999.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8189..8728
                     /gene="rplE"
                     /locus_tag="ACHWN2_002500"
     CDS             8189..8728
                     /gene="rplE"
                     /locus_tag="ACHWN2_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR8932000.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8743..9048
                     /gene="rpsN"
                     /locus_tag="ACHWN2_002501"
     CDS             8743..9048
                     /gene="rpsN"
                     /locus_tag="ACHWN2_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR8932001.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9082..9474
                     /gene="rpsH"
                     /locus_tag="ACHWN2_002502"
     CDS             9082..9474
                     /gene="rpsH"
                     /locus_tag="ACHWN2_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR8932002.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9487..10020
                     /gene="rplF"
                     /locus_tag="ACHWN2_002503"
     CDS             9487..10020
                     /gene="rplF"
                     /locus_tag="ACHWN2_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR8932003.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10030..10383
                     /gene="rplR"
                     /locus_tag="ACHWN2_002504"
     CDS             10030..10383
                     /gene="rplR"
                     /locus_tag="ACHWN2_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR8932004.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10398..10901
                     /gene="rpsE"
                     /locus_tag="ACHWN2_002505"
     CDS             10398..10901
                     /gene="rpsE"
                     /locus_tag="ACHWN2_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR8932005.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10905..11084
                     /gene="rpmD"
                     /locus_tag="ACHWN2_002506"
     CDS             10905..11084
                     /gene="rpmD"
                     /locus_tag="ACHWN2_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR8932006.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11088..11522
                     /gene="rplO"
                     /locus_tag="ACHWN2_002507"
     CDS             11088..11522
                     /gene="rplO"
                     /locus_tag="ACHWN2_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR8932007.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11530..12861
                     /gene="secY"
                     /locus_tag="ACHWN2_002508"
     CDS             11530..12861
                     /gene="secY"
                     /locus_tag="ACHWN2_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR8932008.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACHWN2_002509"
     CDS             12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACHWN2_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR8932009.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13156..13512
                     /gene="rpsM"
                     /locus_tag="ACHWN2_002510"
     CDS             13156..13512
                     /gene="rpsM"
                     /locus_tag="ACHWN2_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR8932010.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13529..13918
                     /gene="rpsK"
                     /locus_tag="ACHWN2_002511"
     CDS             13529..13918
                     /gene="rpsK"
                     /locus_tag="ACHWN2_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR8932011.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13952..14572
                     /gene="rpsD"
                     /locus_tag="ACHWN2_002512"
     CDS             13952..14572
                     /gene="rpsD"
                     /locus_tag="ACHWN2_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR8932012.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14598..15587
                     /gene="rpoA"
                     /locus_tag="ACHWN2_002513"
     CDS             14598..15587
                     /gene="rpoA"
                     /locus_tag="ACHWN2_002513"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR8932013.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15628..16011
                     /gene="rplQ"
                     /locus_tag="ACHWN2_002514"
     CDS             15628..16011
                     /gene="rplQ"
                     /locus_tag="ACHWN2_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR8932014.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4141 a         3784 c         4292 g         3879 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt gctattgtaa
      721 tcataaccat tctcatttac actttgtgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagccc acgcttattt tttgacatcc tgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atctgacctg cgattagaac
     1321 tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc cgacagcgtt catgactatg
     1381 ttagccgcga tatgatgatc gagatccttg ctgatgaaga aggtcatatc gactggctcg
     1441 aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa ttacctgcaa tcacaaatta
     1501 aagttaaaga ttaacggcct atcagcaaac tatcccagcc tataacgaaa cccgcagccg
     1561 ccagaaatgg cccaaaaggc agcgggtttt ttaatgattc ttttcctctc atgagcagac
     1621 caataactac ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt ggtaagaagg
     1681 cccatgaatg ctaggcgcca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgtcg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaacaa
     1801 ttgcccccca taacgcatcg gctaaaccat caggatgaca aacttgatag aacaaaagac
     1861 ctgaccagag taacgggcac gtaaatctgt caggtaacaa gccatgcttt gcatcccaga
     1921 aaaaaagtag ggctgataaa caagcgtaca aaattagaaa gggaagggta gtgtccataa
     1981 aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagag
     2041 agcgctgatt tgttaacctg attccggtga aaaacgctat cttcacaacc ttacaaattt
     2101 cgtggaatgc agctcgcaat ctgcgcgttg cgattaattt ctacccgatg cttgcgtcag
     2161 gctcagattt gcgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctgaacc
     2221 tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg
     2281 ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa
     2341 ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca
     2401 tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca
     2461 ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc
     2521 gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga
     2581 catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg
     2641 tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca
     2701 atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt
     2761 tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg
     2821 gctaacgatg gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc taaccgtgtg
     2881 accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg
     2941 gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg
     3001 tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt
     3061 accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac
     3121 cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa
     3181 atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt
     3241 gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac
     3301 ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga
     3361 cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct
     3421 ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa
     3481 gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag gcaccggccg
     3541 tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc
     3601 tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag
     3661 catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga
     3721 agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct
     3781 gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcagaaggt
     3841 tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt
     3901 aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt
     3961 ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa
     4021 tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct
     4081 gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa
     4141 gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc
     4201 ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata
     4261 caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta gttaaagtgg
     4321 ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat
     4381 ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc
     4441 aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac
     4501 gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac aaagacggtg
     4561 aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg
     4621 ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta
     4681 ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta
     4741 cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg
     4801 aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc
     4861 atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg
     4921 ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact
     4981 ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca
     5041 acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc
     5101 atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag
     5161 aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc
     5221 tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta
     5281 tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat
     5341 cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa
     5401 ctattgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc
     5461 tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag
     5521 cggctgtaca ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg
     5581 gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga
     5641 agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc accagccaca
     5701 tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca
     5761 tcctaatggt attcgcttgg gtattgtaaa accatggaac tctacttggt ttgcgaacac
     5821 caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga
     5881 actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt
     5941 aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag acgtagaaaa
     6001 actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt
     6061 tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg
     6121 tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc
     6181 taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg cacgtaccga
     6241 atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac
     6301 ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga
     6361 gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa
     6421 gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa
     6481 attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt
     6541 cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc
     6601 agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt
     6661 cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa
     6721 cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt
     6781 tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac
     6841 cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcatt
     6901 gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag
     6961 gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca
     7021 cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc
     7081 tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt
     7141 ttgtgaaaca cccgatctac ggaaaattca tcaagcgtac gaccaaactg cacgtacatg
     7201 acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt
     7261 ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta
     7321 cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc
     7381 cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc
     7441 gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac agactatgct
     7501 gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc
     7561 gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc
     7621 gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg
     7681 tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa
     7741 caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg
     7801 tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca
     7861 tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta
     7921 aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattattgaa ggtatcaacc
     7981 tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa
     8041 aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg
     8101 accgtgtagg ctttagattc gaagacggca aaaaagtccg tttcttcaag tctaacagcg
     8161 aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga
     8221 agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt
     8281 cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga
     8341 taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg
     8401 caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct
     8461 gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat
     8521 ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg
     8581 tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg
     8641 tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc
     8701 tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg
     8761 aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa
     8821 ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc
     8881 aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa
     8941 acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa
     9001 gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt
     9061 gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt
     9121 atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa
     9181 gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc
     9241 gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa
     9301 agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga tgagctgccg
     9361 aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat
     9421 cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaatcggag
     9481 gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt tgacgtaaaa
     9541 atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg tactctcaac
     9601 gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg tgatggttac
     9661 gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat ggttatcggt
     9721 gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg tgcagcggtt
     9781 aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca tcagctgcct
     9841 gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg cgctgataag
     9901 caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga gccttacaaa
     9961 ggcaaggggg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa gaagaagtaa
    10021 ggtaacacta tggataagaa atctgctcgt atccgtcgtg cgacccgcgc acgccgcaag
    10081 ctccaggagc tgggcgcaac tcgcctggtg gtacatcgta ccccgcgtca catttacgca
    10141 caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt agaaaaagct
    10201 atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt gggtaaagct
    10261 gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg ttccgggttc
    10321 caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg ccttcagttc
    10381 taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg caggaaaagc
    10441 tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc tccttcacag
    10501 ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt aaagcgcgtg
    10561 aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg attaacgtcg
    10621 cgctgaataa cggcactcta caacaccctg ttaaaggtgt tcacacgggt tctcgcgtat
    10681 tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg cgcgccgttc
    10741 tggaagtcgc tggggttcat aacgttctgg ctaaagcata tggttccacc aacccgatca
    10801 acgtggttcg tgcaactatt gatggcctgg aaaatatgaa ttctccagaa atggtcgctg
    10861 ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca aagactatta
    10921 aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca acgctgcttg
    10981 gcttgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct gctattcgcg
    11041 gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg cgtttaaata
    11101 ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt ggtatcggtt
    11161 ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaagtctcgt tctggcggtg
    11221 gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg ccgaaattcg
    11281 gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctatctgac ctggctaaag
    11341 tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc ggtatccaga
    11401 tcgagttcgc gaaagtgatc ctggctggcg aagtaacgac tccggtaact gttcgtggcc
    11461 tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa atcgaggaat
    11521 aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg tggcttaggc
    11581 gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg tattggctct
    11641 tttattccga tccctggtat tgatgccgct gtacttgcca aactgcttga gcaacagcga
    11701 ggcaccatca ttgagatgtt taacatgttc tctggtggtg ctctcagccg tgcttctatc
    11761 tttgctctgg ggatcatgcc gtatatttcg gcgtcgatca ttatccagct gctgacggtg
    11821 gttcacccaa cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg taagatcagc
    11881 cagtacaccc gttacggtac tctggtgctg gcaatattcc agtcgatcgg tattgctacc
    11941 ggtctgccga atatgcctgg tatgcaaggc ctggtgatta acccgggctt tgcattctac
    12001 ttcaccgctg ttgtaagtct ggtcacagga acgatgttcc tgatgtggtt gggcgaacag
    12061 atcactgaac gaggtatcgg caacggtatt tcaatcatta tcttcgccgg tattgtcgcg
    12121 ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga cctgcacttc
    12181 ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt tgtgtttgtt
    12241 gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg tcgtcgtgtc
    12301 tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt aatcccggca
    12361 atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt cgggggcggt
    12421 actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca accgctttat
    12481 gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc gttggttttc
    12541 aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc aggaattcgt
    12601 ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac cttggttggt
    12661 gcgctgtata ttacctttat ctgcctgatc ccggagttca tgcgtgatgc aatgaaagta
    12721 ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat ggactttatg
    12781 gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa gaaggcgaac
    12841 ctgaaaggct acggccgata attggtcgcc cgagaagtta cggagagtaa aaatgaaagt
    12901 tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg atggtgtcat
    12961 ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat tttttcgcat
    13021 atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt ttgtatgtct
    13081 gtacgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt acatattaaa
    13141 tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca taagcatgcc
    13201 gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc catcctggct
    13261 gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca aatcgacacg
    13321 ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga aatcagcatg
    13381 agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg tcgtggtctc
    13441 ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc gcgcaaaccg
    13501 atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg cacgtaaacg
    13561 tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca acaacaccat
    13621 cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg gtggttccgg
    13681 tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag agcgttgcgc
    13741 tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag gtccgggtcc
    13801 aggccgcgaa tctactattc gtgctctgaa cgccgcaggt ttccgcatca ctaacattac
    13861 tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc gcgtataacg
    13921 cctcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc ctaagctcaa
    13981 gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg cgatcgatac
    14041 caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc gtctgtctga
    14101 ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg tgctggagcg
    14161 tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg gtgaaaacct
    14221 gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct tcggtgccac
    14281 tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg gtcgtgttgt
    14341 taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg agaaagcgaa
    14401 gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa agccaacttg
    14461 gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg agcgttctga
    14521 tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt aaagcttagt
    14581 accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg
    14641 atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct
    14701 ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg
    14761 cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc
    14821 aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca
    14881 aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata
    14941 tcacccacga cggtgatgtt gaaatcgtca agccgcagca cgtgatctgc cacctgaccg
    15001 atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg
    15061 cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg
    15121 catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc
    15181 gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag
    15241 aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac
    15301 gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc
    15361 gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta
    15421 tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc
    15481 ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg
    15541 gcatgcgctt ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatcacaggt
    15601 taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg tcaactgaac
    15661 cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact ggttcgtcat
    15721 gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt tgagccgctg
    15781 attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc ccgtactcgt
    15841 gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc gagccgtgcc
    15901 ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc gccgatggct
    15961 tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta atctgaagca
    16021 atgtaaaaaa acccgccccg gcgggttttt ttatacccgt agtatcccca cttatctaca
    16081 atagctgtac tctttt
//
LOCUS       DAZSNO010000082        15921 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZSNO010000082 DAZSNO010000000
VERSION     DAZSNO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15921
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.082"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..756
                     /locus_tag="ACHWN2_002515"
     CDS             <1..756
                     /locus_tag="ACHWN2_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFR8932015.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACHWN2_002516"
     CDS             complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACHWN2_002516"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR8932016.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1792..1995)
                     /locus_tag="ACHWN2_002517"
     CDS             complement(1792..1995)
                     /locus_tag="ACHWN2_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR8932017.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACHWN2_002518"
     CDS             complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACHWN2_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR8932018.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4588..5100)
                     /locus_tag="ACHWN2_002519"
     CDS             complement(4588..5100)
                     /locus_tag="ACHWN2_002519"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR8932019.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="ACHWN2_002520"
                     /pseudo
     CDS             complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="ACHWN2_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            complement(6903..7840)
                     /locus_tag="ACHWN2_002521"
                     /pseudo
     CDS             complement(6903..7840)
                     /locus_tag="ACHWN2_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="ACHWN2_002522"
     CDS             complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="ACHWN2_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR8932020.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            complement(8767..10122)
                     /gene="hpaX"
                     /locus_tag="ACHWN2_002523"
     CDS             complement(8767..10122)
                     /gene="hpaX"
                     /locus_tag="ACHWN2_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR8932021.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            complement(10144..10947)
                     /gene="hpaI"
                     /locus_tag="ACHWN2_002524"
     CDS             complement(10144..10947)
                     /gene="hpaI"
                     /locus_tag="ACHWN2_002524"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR8932022.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            complement(10958..11761)
                     /gene="hpaH"
                     /locus_tag="ACHWN2_002525"
     CDS             complement(10958..11761)
                     /gene="hpaH"
                     /locus_tag="ACHWN2_002525"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR8932023.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(11829..12209)
                     /locus_tag="ACHWN2_002526"
     CDS             complement(11829..12209)
                     /locus_tag="ACHWN2_002526"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR8932024.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            complement(12219..13070)
                     /gene="hpaD"
                     /locus_tag="ACHWN2_002527"
     CDS             complement(12219..13070)
                     /gene="hpaD"
                     /locus_tag="ACHWN2_002527"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR8932025.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(13072..14538)
                     /gene="hpaE"
                     /locus_tag="ACHWN2_002528"
     CDS             complement(13072..14538)
                     /gene="hpaE"
                     /locus_tag="ACHWN2_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR8932026.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(14535..15824)
                     /gene="hpaG"
                     /locus_tag="ACHWN2_002529"
     CDS             complement(14535..15824)
                     /gene="hpaG"
                     /locus_tag="ACHWN2_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR8932027.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPSDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
BASE COUNT         3753 a         4148 c         4166 g         3854 t
ORIGIN      
        1 tatactaaga ttttagagaa tgattttgaa cacccttcaa tacgacgaaa aatagcatcg
       61 ctaagtaatt taggggtaga tgagtggatt cctccggtta tggcattcat gaatcgaatg
      121 gctcgtacag aagattttaa tttagatgat ttttctcaat ttattaccgc ctttgagaag
      181 gtatatatgc atgggtggtt aaaaaaacaa attaaaagcc aaagagaaat ggtttgttat
      241 tctgctctgg tagcaataaa taatgatatg ccttttgatt ctgtgataaa tcagataaac
      301 caacatgcag ataattcagg ttttatagct gctctggatg aagatttata tgagcctcgt
      361 ccgaatcaag tgaacttaat aaaagcaatt ttgcttcgct tagatatgga gcaacaagat
      421 gagagcgtga ttaaaaccta tacggggaga atcacaattg agcatattct tcctcaagca
      481 ttagttaatg aatactggat taatagattc caacctcaag agcatgtcta ctggctgcat
      541 aaaataggta atttaacact cataagtggt tccaaaaatt ctgaaacaca acactatgat
      601 tttatcaaaa agaaatctat ttatgagaaa cttaatagta aaagctcttt ttatttaaca
      661 aaagatgttt gtaattcttc agaatgggga ttggctgaac tgaaaatgag acatgaaaaa
      721 atgaaaacgc aattgaagaa gctttggttg gtctgacata tctaaagagt tccactcgta
      781 ataatgttgc ggtggaactc tcaaaaagtt gatctttccc gaacttactt cctcaatccc
      841 gcaaacgccg cccgaatctc ttcctccggc aactgaatcc cgataaacac cattgtgcta
      901 tgcggttttt catcgcccca cggcctgtcc cagtcggcgc tgtagaggcg ctggacgccc
      961 tggaacagca ggcggttagg ttcgccgtca atccacagca tccctttgta acgcagcagt
     1021 ttatccgccg attccagcag caggttttcc atcacgcggg aaacttcgct gatatctacc
     1081 gggtaatcca gttccaccac aattgacgaa atatcgtttt gtttatccgc gataaagtgg
     1141 aaacgcggtt tggtgctgac gacgttctct tccagcataa aaccgttggt gttgaacagc
     1201 aggcccaggt cgatgtcgcc gtgggtgacg gtgtagaccg gtgcgcgggc gttgatgcgc
     1261 gccaggcgtt cacgcagttt ctctgcttcg cctgcgacgt cggttttagt cagcagaata
     1321 cggtcggcgt agcccacctg ggactgggcg atggtgaact ggttcatctg ctcatcggca
     1381 tgtaccgcat ccaccagcgc aatcacgccg tccagcaggt agagctggca taaaatctca
     1441 tgggagaaaa aggtctgaat aatcgggccg ggatcggcca tgccggtgca ttcaatcacc
     1501 agacgatcga actgaatatt gcccttgtcg agattatcca gcaggtcgag tagtgcgtcc
     1561 tccagctcgt tggagcgcga acagcagatg cagccgttgg tcagcgtttt gatctgcgtg
     1621 gcgcggtcgc caatcagttg gtcatcaacg gagacttcgc cgaattcgtt ttcaatcacg
     1681 gcaatcttgt agccgtgttg ttcgttaaga atatggcgca gcagggtagt tttgcccgcg
     1741 cctaaaaaac cggtgagtag ggtaactgca atcgggttca tgctctctcc attaacaaca
     1801 gcgcataccg cctttaccat cgccgccgta gcgcgcgttc tggcgttcgc ggaagaattc
     1861 ttcatagctc atgtacggct tgtcgggatg gttggtcttc atatgctcga cgtagttgtc
     1921 gtagtctgga ataccaatca acatcttcgc cgcctggccg agatattttt ttgcctgtcc
     1981 taagttacca aacatagttc actctgataa gaacaaagcc ccgccgaagc ggggctaaac
     2041 acggttagtg gtgcgaagag atcttcacgc cgccttccgg gattggaacg tacggtgttt
     2101 ctttgtcagt acgtttgtcg ctgttacgca ccgcaagcca ggttttgaaa ccgtagaaga
     2161 tgatgctgta caccacaatc aggaacagaa tactcaggcc tgcgttggtg tagttgttca
     2221 caacgatgtg gttcatgttg gcaatctgct gcgccgtcag gtcagtaccg ttagcaatct
     2281 tctctttgta ctggcttgcc atgtagaaga agccttccat ctgcgggttg gtgctgaaca
     2341 gtttcagacc cagcgcccag gtggtgcaga taagcagcca tacagccgga acaacagtta
     2401 cccagatgta ttgggtgcgc ttcatcttaa tcagcacaac ggtgcccagt accagcgcta
     2461 cggctgccag catctggttg gagataccga acagcggcca caggctctta acgccgccca
     2521 gcggatcgac cacgccctga tacagcaggt agccccacag acccacacag cccgcagtac
     2581 cgatgatacc ggcaaccaga gagtcggttt ttttcaggaa cgggatgaag ttacccagca
     2641 ggtcttgcag cataaagcgg ccagaacggg tacccgcatc cagcgcggtc aggatgaaca
     2701 gggcttcgaa cagaataccg aagtgatacc agaagcccat gtcagccatc ggcagcactt
     2761 tgtggaacac gtgagcgata cctaccgcca gcgtcggcgc accacctgca cggttcagga
     2821 cagaaggctc accaatgtct ttcgcggttt gcaggatctg ctctggcgaa atcacgaagc
     2881 cccaggagct gacggtcgct gccgcgtgtg cggtaacgtc tttcagctgc gccatgatga
     2941 tcggcgcgtt ctcgccaccc atttcatgca ggttaggcat ggtgatgcca aggccagcag
     3001 gcggggtgtt catcgcgaag taaagacccg gttcgatgat ggacgctgca accagcgcca
     3061 taatcgccac gaaggactcc atcagcattg cgccgtagcc aatgaaacgc gcgtcggttt
     3121 cgttagccag cagtttcggc gtcgtaccgg aagagatcag cgcgtggaag ccagataccg
     3181 caccacaggc gatggtgatg aacaggaacg ggaacagagc gcctttccac agcgggccag
     3241 taccgtcaat gtactgggtc atcgcaggca ttttcagttc cgggttcagc accacgatac
     3301 ccagcgccag gccgacgata acgccgattt tcaggaaggt tgccagatag tcgcgcggtg
     3361 cgaggatcag ccacaccggc agcagtgcgg aaacaaacgc atagccaatc agcgcgaagg
     3421 taatggtggt gtctttaaag gtcagtgccg gaccccagta cggatcgtga gcaatcacgc
     3481 caccgaagta gatagaggca accagcagca cgataccaat aacagagact tcacccacac
     3541 gccccggacg gataaagcgc atgtagatac ccataaacag cgcaatcggt acggttgagc
     3601 aaacggtgaa gacaccccac ggactttcgg ccagggcttt aaccacaatc agcgccagaa
     3661 cggcgaggat gatgatcatg attaagaaac agccaaacag cgcgatagtc cccggtactg
     3721 gccccatctc ttctttgatc atctcaccga gagatgcgcc gttacggcga gaggagataa
     3781 acagcaccat aaagtcctgt accgcaccgg ccagcactac ccccgccagc agccacagcg
     3841 tgccaggcag gtagcccatc tgcgcggcga gaaccggtcc caccagcgga ccagcaccgg
     3901 cgatagcggc gaagtggtga ccaaacaaca cgtaacggtt ggtcggaacg tagttcagac
     3961 cgtcgttgtt aataaccgca ggcgtcgcgc gcgtggggtc gagtttcatc accttctggg
     4021 cgatgtacag actgtagtag cgatacgcca ccagatacac cgacacagag gcgaccacga
     4081 tccacagggc gctgacgtgc tccccccgac gtaatgcaac taccgcgagg cagaatgcac
     4141 cgatgattcc gagaatcacc cagggtatgt gcttgaatag ttttttcgta tccatagtaa
     4201 aacctggcat tttactcgtc gtcttttggc ttacaggtgc ggcagctgcg ttcgttcacc
     4261 tctgacactt acttttgtaa gctcccgggg attcactcac ttgctgcctt cctgcaaacc
     4321 aaaatccata gagtaatttg ttaatcatag gccgaagccg ttctaggttt gcgttgcgtt
     4381 tgaggaggta aattgaccgc tatcctgcag tgatgttgcc agagatacgt gcgcgtaaag
     4441 taaggtaaat aactgagtgg ttattttagg gatgtaagcg gtcaggaatg gcggtgagcg
     4501 gttgggcaag aattattcat cgggtagata tgtgattgag atcacaataa aaatgcccct
     4561 tcgacgaatg cgtgaagggg ctggagctta aatcgcagct tccatttcca gcatcaccgg
     4621 atggaaacgg cgtttaaagt aaataagtcc gtgaccttct gcactgagga tgatgttttt
     4681 aatctccacc agatacacca gatgtgtgcc aatcgcctgc acatcgcgga tctctccttc
     4741 aagactggcc agcgaacctt ttagcaccgg ctgcgccagc ggaccttttt gccagcatga
     4801 gaggctaaaa cgctcttcca tcgccatgcc tgtcatgccc gcgaagtggc gcgccatcag
     4861 ttcctgctca tggttgagga cgttgacgca caacttgccg ttgccctgaa aaaccgggtt
     4921 catcgcactg ttggcgttaa tgcacaccat cagcgacggt ggtgtatccg tgaccgagca
     4981 gacagccgtt gccgtaatcc cgcattgtcc ggcgtcgccc tcggtggtga taatatttac
     5041 cgctgccgac aggctggcca tcgcgtcatg aaagcgcagg cgttgttcat ctaattgcat
     5101 cttaacctcc tgctgcgtta tttcagcagc ttatccagca tgttgatatc gtcgttgttg
     5161 tgcaggtgcg gcacagtcca gccgttctgg tcgtattccg acaggcagcg atcaaccatc
     5221 gccatcatct tgtccatatt gccggagctt tgtgcctggc gcagacattg caggcgaatc
     5281 tcatcctggc taccggagta gttgatttca tacagctcgt gacgaccacc aaattcgctg
     5341 ccaatggcgt cccacatcag tttgaggatc ttgatgcgct ggacgtgatc cataccgttc
     5401 gaaccgcgca catacttcgc cagatactgg tcgatctgcg gattgttcag gtcacgggca
     5461 ctggaaggga ggtagatcag gccactggta acgttgcgtt ctataatgtt tttgatcttc
     5521 gcgtaggcca ttggtgccag tacgcgatag gtttgcagtg cggcatgatc cggtaaataa
     5581 gccccgttga cccacggcgt cgcttctgaa cacatcgagt cactcaatgc ccagaaggtg
     5641 ttgcgccacg ccaccacttc accgagatcg gcctgcacac cacggaactc cagggtgccg
     5701 gtacattcga gtgatttttt cagcagtgcc gtaatgaagt cgagtttcac tgccaggcgc
     5761 acacaggctt gcagcggata catacgggcg aaaccgcctt ccatcgtcca gcgacggcag
     5821 cgatcaaaat cgcggtagat cagcacgttt tcccatggga tcagtacgtt atccatcacc
     5881 agaatcgcat cgttctcatc gaagcggctg gagagcgggt agtcgtatgg cgagccggta
     5941 gcacccgcga ccatctcata agaggcgcgg gagattaatt tcacgccatc ggcatccatt
     6001 ggcgcaacga acatcagtgc gaagtccggg ttttcgccca tcacttgtgc cgagccgaag
     6061 ccaatcatgt tgtagtgagt cagcgccgag ttggtggcaa ccactttcgc accgctgacg
     6121 ataatcccgg cgtcagtctc tttttccagc ttgatgtaaa cgtctttcac tttatcggtc
     6181 ggcaaatgac gatcggtggg ttaacaatcg cgtggttaaa gtagaggcca gtttcctgaa
     6241 tacgggtata ccagttacgg gcgttctgct cgaactgacc gtaaaagccc ggattcgcgc
     6301 ccagtgcgca accgaaagcg gctttgtagt ctggggtacg gcccatccag ccatagctca
     6361 ggcgtgacca ttcagcgatg gcgtcgcgtt gctggcgcag gtcgtcggca cttttcgcca
     6421 cgcggaagaa tttatgggta tagccgccgc tgccggtgtc ggtgttccaa cacagagagt
     6481 cctgcatctc cggtttgtgc agcgcgtcgt acagttgggc aacagacgct gccgcattac
     6541 gaaatgccgg atgagtggtg acgtctttca ctcgctcgcc atagatatag atctcgcgac
     6601 catcctgcag gcttttcaga tactcttccc cggtgaaagg acgttgggta ctggcgcgga
     6661 aatcttctgg tttcatgtcg acctctacag tattaatcgg aatacgattg ttaattttgt
     6721 gtgttgtttt tgttatttaa ttgaagcgtt gaacgcggtt gtcgggaaga gacgtttcag
     6781 gcgaacgttg gtacttttcg acagcaaggt ggagttaaat agtaaactgc gatcgtgatc
     6841 aacttacagt aaggcaaatc ataatattcc ccgctaacaa gggctaacgg ggacggaatt
     6901 ttttatgggg cgaatggaat ggtaatcgtc atattttcat gaccaacgac gataggacct
     6961 gaatagtttt ctgtgcttct tgcttccatg tttcatccgg aacggtgatt ggtaccaggt
     7021 ggctaaggat gagttttttc acttttgctt cattggcaat tttgcctaca tcctcaatag
     7081 tcgtatggtg agaagcaatg gcttttgcca gtgttttacc attgccaata ctgtctgcca
     7141 gcttagcgac ggagggctca tgtacggcct catgcacaag tatatcagcg ccttctgaga
     7201 aagttgccag tgggggaaag tacgaggtat ctcctgagaa gacaattcgc ttgccttgtg
     7261 tgtcgaagcg ataagcaaaa gcctcaccgt cagggaaagg agaatgaggg acctttaatg
     7321 cagtcacggt cacggtatca tctttataaa ctacgccttc accaacatct tgagctgtaa
     7381 cgagtttgcg aggatcgggt tttccttctt cttccatacg cagtgtgatg tcccgatcga
     7441 agacctgcca actggcagcg gtcatggctc gggtgcctgg cggaccgaat acatgaatag
     7501 ttttgtcctg cagaccgcta gcccatgcat tcattaagac tgatggatag tccagaatat
     7561 gatcactgtg taaatgggtg agtaaaatat tatcaatctg gcgcagtgga atattatttt
     7621 ttaccagttg taagctggcc ccgtaaccag catcaattaa ccaggtttta ttgccaataa
     7681 taagtgccgt tgccatcggt aaacggtcag cttttaataa agaagggcca cctttcgttc
     7741 caagtagtac aattttcatt tattgcggtt cggaggcgta ggtgaatgcc actggcgcaa
     7801 tcaggcacgc cacaaatagt gagcgtaaag atattttcat gtcgagtctc tcaggttgat
     7861 aaatggtcag cgaccacgga attgtgttgg tgagcaaccc atcattcggt tgaaaaaacg
     7921 ggcgaaatag gcgggatctt tgtagccaag atcaagggct atctggttga tgctttgctc
     7981 gctatagagc agaagccgtc tggcttcccg gctcactcgt tcgaaaatca atctcttcgg
     8041 cgattgattg gcaaaacgtc ggcacagctc ggtaagccgt gattcactca taccgagcat
     8101 tgttgcatat tcagggacca ttaaatgttg gcgataattt tcgtcaatca gccgattaaa
     8161 cttctggaat aaacacatct cgccgcgcac gctgcatgtt gaaatatcat ttggtggtgc
     8221 ttcacgcagt aggattgtaa aaatactttg cgccagtgat tgcaatagaa tttcccgtcc
     8281 ttgcttattt tgttgaaact ccagggatat tatcggccaa taatgattta atgccgccag
     8341 tgtatgttcc attccctcca gtgacagaca gataccttgt agcattgcgg catctccact
     8401 accaggccag agcttttcta ccaggggcca aattaactcc tgacgtacgg tcagaacatg
     8461 cccatcgcta tcaggatcag taaagaaagt atgtggcacc gatggtggtg ttagtacaaa
     8521 aagcggtgcg cgcacagaat agaagtgctc atccagttgt aaagtaatgc gtccagtgac
     8581 cagataatgg agttgaaaat atctatcgtg ccagtgaggg cgcatatcgc gtccaaagaa
     8641 attcgctaac cgggcaaatg tttcataatg cacatcttca ccagcgtagc gggcatcata
     8701 ttcccggacc aaatcaatat tttcataacc atgttcaaag atagcttcac tggacatcat
     8761 gactccttat tgcggttttg gtgcgataag ttcagatgaa agagagtgtt cccgactccc
     8821 catagggatt cgggtcatga ctaacgcgcc cagtaccagc aatcctgcta caaaccacaa
     8881 acctgagcta aaactgccgg ttgcatcttt caaaataccg ataagtaatg gactgactgc
     8941 tgaacctaca ttgccgacag cattgatgac agccatcgca acggcacgcg attgcaagct
     9001 tataacttga tccggtgttg tccaaaagat agtcatcgcg gtaaatgatc ccattgaggc
     9061 cataatgatg ctgctcagtt gaatgaggct gtgatctgtc gcagaagcga gtaaccatcc
     9121 ggcagcggca aacaggtagg gaagaatcgt atgctttttg cgttctttaa gacggtcgga
     9181 acgacgactc caccagatca tgccaaaaat tgtgcagaat tgggggatgg ccgccagcaa
     9241 accaatcatg atgttgctac tgccagtatt gaaactctgt aggatttgcg gcgtccagat
     9301 attaatcgca ctcagagtat tagtcaggca aaaataagca agtgtatata gcatgatggc
     9361 tggggtcagg acttcacgta aggttgagcg tggaacagat gcatgggcaa atgcactctc
     9421 ttgttcacgg agaatcatcg tttttaaagc ctgtttttcg ttttcatcaa gccaggtcgc
     9481 ctggtctggt gtgtcgttga gataaaacca ggtgacgaca ccaagaatga ccgatggcag
     9541 accttccagc aaaaagagcc attgccagcc ttttagattc cacagtccat ccaaggccag
     9601 aatgtagcct gaaaggatca atcccagcat catagtgact ggcatcgcta tcataaataa
     9661 ggcattggct ctggcccgat gatatgccgg gaaccaccag gtcagataaa ccaaaatacc
     9721 aggtaaaaaa cccgcctcag caataccaac cagcatacgc agtagataga gtgtttcggg
     9781 gcttgttgca aacattgtgc aggtggaagc aattccccag accaccatga tcccagcgat
     9841 ccatcgacga gcgccaattt tcgctaacat catattactg gggatcccgc acatgacgta
     9901 cgtgacataa aagagtgttg cggcgaggcc aaacattgtg gacgtcaggc caagatcctt
     9961 gcccattgtc agtccggcaa atcctatatt gatgcgatca agaaacgaaa aaacaaacag
    10021 aataaaaaga aaaatgatta ggcgacgaaa taatttatta atgacacgat gttcaatttc
    10081 aacagtttta ttatctttat attgcagggt agaggtcgtc atgatgcgct ccacatcgta
    10141 cgtttagtag acagagttgt tgttttgctt cgccatcgaa atagaagaac tattaccaaa
    10201 ccgtgctgcc agcgcttcgg cggcgcgggc gagcagggtg gtgtcaacgc cgacggcgac
    10261 aaacagcgcg cccagttcca gatagcgttt tgccagttgc tcattggcga tcaggatccc
    10321 cggcgctttg cccgcttcgc ggatctgcac gatcgcctgc tcaatggcgg cctgtacttc
    10381 cgggtgctgc ggattaccgg cataacccat atcggcgctc agatccgccg ggccgataaa
    10441 cacgccgtcg accccttcca cgtccagaat ctgcggtaag ttcttcattg cctcacgcgt
    10501 ttcgatctgc accagcacgc acatttgatc gttggctttt tgcaggtaat caggaatgcg
    10561 attccagcgc gaggcgcgag ccagcgcact gcccacaccg cgaataccgg cggggggata
    10621 acgggtggcg cgtaccgctt tacgggcttc gtcggcgttt tgtaccatcg gcaccagtaa
    10681 ggtttgtgtg cctacgtcca gcagttgttt gatttgcacc ggatcgttcc acgatgggcg
    10741 taccactggc tggctggggt agggcgcaat cgcctgtagt tgggtgagca cggtttgcac
    10801 gttattcggc gcgtgctcac cgtcgatcaa taaccagtcg aatcctggtc cggccagtaa
    10861 ctccgcgctg tagctgctgc tcagccccag ccataatcca atctgcggac ggcctgcttt
    10921 cagcgccgct ttaaaactgt tttccatcgt tctctcctta aacaaagcgg cagctaatgg
    10981 agcccatgtt gccgtaatcg acgtggaagg tgtcgccctt acgcgccgga accgggaggg
    11041 tgaacgaacc accgagaatg atttgcccgg tttccagttg tacgtcatac ggtgcgagtt
    11101 tgttcgccag ccaggccacg ccgtttgccg gatgattcag cacaccagcg gcgacgccgg
    11161 tttcttcaat cacgccattg cgatacatca gggcggagat ccaacgcaga tccagttcgt
    11221 ctggtttgat cggacgacct ccgaggatca ctccggcgtt ggcggcgtta tcggaaatgg
    11281 tgtcgaatac tttgcgcggg cgctgggttt ccggatcgat gttgtggcag cgagcgtcga
    11341 tcagctccag cgccgggatc acatagtccg tggcgttgta aacgtcgaac agcgtgcagt
    11401 ttggcccacg cagcggtttt gccagcacaa aagccagctc cacttcaatg cgcggcacga
    11461 taaagcgatc ggtcgggata tcgctgccat cgtggaagaa catgtcgtcc agcagtgcac
    11521 cgtaatccgg ttcgctgatc tgcgagcttg cctgcatcgc tttcgaggtc agaccgattt
    11581 tgtggccttt cagcgtgcga ctttcggcga ttttcaggcg aacccattcg cgctgcacgg
    11641 cgtaggcgtc ttcgatggtg atctccgggt aatccagcga gatcgcgcgg atctgttcgc
    11701 gctgtttttc tgcctgatcc agacgctggg cgatcagggt gtgggtgtgt ttatcgaaca
    11761 tagcgatatc ctgtggcatt gccggatgcg gcagcgcctt atcatccggc ctggaagctg
    11821 gtgcaaattt atttaaacaa cgcgtgtacg ttgttctgtt tgaaattcaa cgtcgggtgc
    11881 agctctgcaa tctcaaatga aagcgctagg taacggctct ccatcagtga ggcgaagtgc
    11941 gttttaatca gatcaaacag catttcgccc acttcctgac ggctttccag actgcgtccg
    12001 ctgccgattt ttagcgtcat atgcacaaag gcgtaatcgt gcttgccatc agccatttgc
    12061 caggtatcga tccagtgcgc ccggctacgt attcccccaa gcggaaaaat ccccgttgcc
    12121 gccagtgcag tgttaacact ggcaaacagc tcaggtaagc gggcttcttc acgaatattt
    12181 tcagtacatt ccacgataaa gtgcggcata aaacgtcctt acgcgggcag cgggaaaaca
    12241 gcgttaacct gaccggtgcc agagcttggg aacagctcgg taataaactc caccttgccg
    12301 tcgtatttat cccagccgag catccccagc agcatcaccg tgtcgtgcat attgccttcg
    12361 ccgtagcagt agtcggcgta ctccggcagc atattgcaaa actctttgaa ctggccttcg
    12421 cgccacagct tcaccacacg ctcgtccatc tggcggtcga actcgcgggt gtagctgttc
    12481 atcccttctt ccgcacgctg atcgtcaata aagcggtgcg ataacgaacc gctggcaagg
    12541 accgccacgg tgccgtcgta ctgttcgatc gctttcagaa tcgcttcgcc cagcttgcgg
    12601 ctgtcggcaa aatcgtgaac cgtgcagaaa gccgaaatgg agaccacttt gaagtgcttg
    12661 tcttcattca tgtagcgcat cggtaccagc gtgccgtatt ccagtttcag gctgggaata
    12721 ttgtgcgctt ttgcccgcac gccgagcttt aaggcttcct cggcaattag ctgccccaac
    12781 tccgggttgc cctcgtagtt gtaggtcatg tcgcgaataa aatgcggcag ctcgttgctg
    12841 gtgtagacgc cttcaaaatg gtctgcacag ttgatgtgat aagcactgtt gaccagccag
    12901 tgagtatcga aaacgataat ggtatcgacg cccatttccc ggcaacgctt gctgatctct
    12961 ttatgcccgt cgatcgcacc ctggcgacaa ccgtggtttt ttcctggtag ttcagagaga
    13021 tacatcgacg gtacgtgcgt gatcttggcg gctaacgcta acttacccat atcagactcc
    13081 ccatttcgga attggatggt cgcccatgga aatgcagacg ttcttcattt ccgcgaacac
    13141 ttcgaagctg tactcaccgc cttcacgccc ggtgccggag gcttttacgc cgccaaatgg
    13201 ctggcgcagg tcgcgcacgt tctgggtgtt gacgaacacc atgcctgctt caatgccacg
    13261 cgccaggcgt aacactttgc tgacatcctg tgtccagatg tacgacgcga ggccgtactc
    13321 cacgtcgttt gccaggcgta agccttcggc ttcgtcttta aacggcagca ggcaggcgac
    13381 cggcccgaaa atctcttcct gggcaacgcg catacggtta tctacgtccg ccagcaccgt
    13441 tgggcgcagg aagttgccgc ctttcaggtg cgcaggcagg tcggacggtt tatccgggcc
    13501 gcccgccaac agggttgcgc cttcttcaat gccgagacgg atatagccgg agactttttc
    13561 ccagtgttgc tgactgataa gcgccctaac ctgggtattc ggatcgttcg gatcgcccac
    13621 gcgcagacgg ttggcgcgtt cggcaaagcg tttaacgaat tctggataga tgctctgctg
    13681 aataaagatg cgcgaaccgg cggtgcagcg ttcgccgttg attgagaaga tggtgaacag
    13741 ggcggcgtcc agcgcacgct caatatcggc atcttcaaaa atcagcaccg gtgatttacc
    13801 gcccagctcc atggagtatt ttttcagccc ggcgtttttc atgatgttgc gcccggtggc
    13861 ggtaccgccg gtgaacgaca cggcacgcac gtcatgatga cggaccagcg catcgcctgc
    13921 ggttgcgccg tagccctgta ccacgtttag cacgcccgcc ggaataccag cttccagcgc
    13981 cagctcaccc aggcggtcgg cggtcagcgg ggagagttcc gacatcttca gcaccgcggt
    14041 attgcccagc gccagacacg gcgcgacctt ccaggtggcg gtcataaacg gcacgttcca
    14101 cggcgacgcc agcgcacaaa cgcctaccgg ttgcaccagc gtgtagttga gcatcttgtc
    14161 gtcaaccgga taggtcttgc cgttcatctg ctggcagact tccgcgaaaa attcaaagtt
    14221 gtgagaagcg cgtgggatca acacattttt ggtctgatgg atcggcaggc ccgtgtccgc
    14281 ggtttccatc gcggcgatct caggcacgtt ctggtcgatc agatcgccca gacggcgcat
    14341 caggcgcgca cgctctttca tcggcagatc agcccatttc gggaacgcct ctttcgctgc
    14401 cgctaccgcc tgattgatct ccgcttcacc gccagaggcc acatccgcca gcacttcacc
    14461 cgttgccgga ttggtagtct ggaagtagtc gttacctgca acatttttgc cgttgatcca
    14521 atgatttact tttttcattt cgctgtttcc tcactcacaa ttcggttcac caggcggccc
    14581 acgccttcta cttccaccac tacttcatcg ccaggcacca cgtcagataa gccttttggt
    14641 gtgccggtgg cgatcatgtc gcccggattc agggtcataa attcgcttaa gtaggcgatc
    14701 aggaagggca cgctgaagat cagatcggcg gtggtgcctt gctggcgtaa ctcgccgttg
    14761 acgaaggtgc gaagggtcag attatgcggg tccgggatcg cctctttcgg cacgatggtt
    14821 gaaagcatcg gcgtcagtcc gtcgcggctt ttgacccgca ggttagggcg gtagtagttt
    14881 tccagatagt cgcgaatggc gtagtcgtta cacacggtgt agcccgcgac ataatccatg
    14941 gcatcggctt cgctgacgtt acgcgcctgc ttgccaataa ctaccaccag ctccgcttcg
    15001 tagtgcatgt attcaatatt gttcgggcgc acggaggtct ggttgtcgcc agtgagggta
    15061 ttcggcgctt tcaggaacac cagcggctct tccggtggct taaattccag ttcgctggcg
    15121 tggtcggcgt agttcaggcc gagggcaaac agcgtaccgt gcgggtgtgg cagcgttggg
    15181 aagctcttgc gcgtggtcac ttcacgttcg tccactaccg gattttccag cggcgggaaa
    15241 ccttctgcga gaacacgcac gcgatcgctt ggctgtattt ccacgcgcgc ctgtggcgtg
    15301 ccgagcagaa tagcatcgct tggattcagc gtggcaaatt cgctcagggc gctcagcagc
    15361 tgtgcggcgt tacgttgtaa atcggcggta ttccagtgat cggcaggacg cccgttgatc
    15421 tcggtataga tagtcagatt atcgacattg ctgagagcca cggtttcgcc aatggggcag
    15481 aatccatcac gacattttgc tttgattgcc ggacggtaaa agctctcttc cggcaggctg
    15541 acgtcgttat ccagcgcata tccggcgatg tactctgccg catcttcttc acgtactttc
    15601 gtcgccgttt tccccacaat cagcgcaaca gtcgcaccgc tcagtacttt ttcaccctgt
    15661 ggaaagggaa tcggttcacc gcaaccaatc accgtattgc gcggtttaat aaaccagacc
    15721 gcagttttag gcggggcttt gtagggagat tgctggaacg cttcctgcca ggcatcaagt
    15781 tggctgcgat ggttcaacgc tacggcgaag atagtgcctt tcattaccac tcctcggatt
    15841 cgatcatctt aatcattaat atgttaatga ttacttttat tcccgattgc gtagtttggc
    15901 aaaagtaagt gatataaatg t
//
LOCUS       DAZSNO010000083        15485 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZSNO010000083 DAZSNO010000000
VERSION     DAZSNO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15485
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.083"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(122..874)
                     /gene="tcyN"
                     /locus_tag="ACHWN2_002530"
     CDS             complement(122..874)
                     /gene="tcyN"
                     /locus_tag="ACHWN2_002530"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR8932028.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(871..1539)
                     /gene="tcyL"
                     /locus_tag="ACHWN2_002531"
     CDS             complement(871..1539)
                     /gene="tcyL"
                     /locus_tag="ACHWN2_002531"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR8932029.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1554..2540)
                     /gene="dcyD"
                     /locus_tag="ACHWN2_002532"
     CDS             complement(1554..2540)
                     /gene="dcyD"
                     /locus_tag="ACHWN2_002532"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR8932030.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2645..3445)
                     /gene="tcyJ"
                     /locus_tag="ACHWN2_002533"
     CDS             complement(2645..3445)
                     /gene="tcyJ"
                     /locus_tag="ACHWN2_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8932031.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3533..4084)
                     /gene="fliZ"
                     /locus_tag="ACHWN2_002534"
     CDS             complement(3533..4084)
                     /gene="fliZ"
                     /locus_tag="ACHWN2_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR8932032.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4130..4849)
                     /gene="fliA"
                     /locus_tag="ACHWN2_002535"
                     /pseudo
     CDS             complement(4130..4849)
                     /gene="fliA"
                     /locus_tag="ACHWN2_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(5014..6666)
                     /gene="fliC"
                     /locus_tag="ACHWN2_002536"
     CDS             complement(5014..6666)
                     /gene="fliC"
                     /locus_tag="ACHWN2_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR8932033.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            6932..8338
                     /gene="fliD"
                     /locus_tag="ACHWN2_002537"
     CDS             6932..8338
                     /gene="fliD"
                     /locus_tag="ACHWN2_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR8932034.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8363..8773
                     /gene="fliS"
                     /locus_tag="ACHWN2_002538"
     CDS             8363..8773
                     /gene="fliS"
                     /locus_tag="ACHWN2_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR8932035.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            8773..9138
                     /gene="fliT"
                     /locus_tag="ACHWN2_002539"
     CDS             8773..9138
                     /gene="fliT"
                     /locus_tag="ACHWN2_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR8932036.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9216..10703
                     /gene="amyA"
                     /locus_tag="ACHWN2_002540"
     CDS             9216..10703
                     /gene="amyA"
                     /locus_tag="ACHWN2_002540"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR8932037.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10737..11150)
                     /gene="yedD"
                     /locus_tag="ACHWN2_002541"
     CDS             complement(10737..11150)
                     /gene="yedD"
                     /locus_tag="ACHWN2_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR8932038.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11337..12542
                     /gene="yedE"
                     /locus_tag="ACHWN2_002542"
     CDS             11337..12542
                     /gene="yedE"
                     /locus_tag="ACHWN2_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR8932039.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            12539..12772
                     /gene="yedF"
                     /locus_tag="ACHWN2_002543"
     CDS             12539..12772
                     /gene="yedF"
                     /locus_tag="ACHWN2_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR8932040.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12879..13549
                     /locus_tag="ACHWN2_002544"
                     /pseudo
     CDS             12879..13549
                     /locus_tag="ACHWN2_002544"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(13589..14797)
                     /locus_tag="ACHWN2_002545"
                     /pseudo
     CDS             complement(13589..14797)
                     /locus_tag="ACHWN2_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            14805..15231
                     /gene="tnpA"
                     /locus_tag="ACHWN2_002546"
                     /pseudo
     CDS             14805..15231
                     /gene="tnpA"
                     /locus_tag="ACHWN2_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         3750 a         4024 c         3830 g         3881 t
ORIGIN      
        1 ttgcaagcca tcgcattctc ttatgttatt aatgagttat gctgatttgt taagcagttt
       61 tatcacgctt gaaatggcgt cccgccccga caggtgaatc gtcggggctg attttttctt
      121 attattgcag cagaagcttc tcgaggaact ggcgggtgcg aggctgctgg ggatcggcaa
      181 ataacgcttt tgctgccccc tgctcgacta tccgcccctg gtccataaag atcgcccggt
      241 ccgcaacatc ccgggcaaag ctcatttcgt gtgtcacaat caccatcgtg cgcttttcct
      301 gcgccagctg gcggatggtg ttcaggactt cgcccaccag ctctggatcc agcgccgacg
      361 ttggctcgtc aaacaaaatc acctcaggac gcattgccag tgcacgcgca atcgcaacac
      421 gctgctgttg accgccagac aaacgacgtg gatagctggt ttctttaccg gccagcccaa
      481 ctttcgccag caactcgcga gcgcgcgccg tggcctcttc tttcggttcg cctttgacga
      541 tcaccggccc ttcaataatg ttttccagca ccgtacgatg cggaaacaaa ttaaagttct
      601 ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag cgatttttgc tgacttaatg
      661 aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt tcccgcttcc ggttgttcca
      721 gcagatttat gctacgtagc aatgtggttt tgccggaacc actcggaccg ataattgcca
      781 ccacttcgcc aggctttacc tcgaggtcga taccgtgcag caccgtctga ccgtggaatt
      841 ttttcaccag gttcttaact tcaatggcac tcattttggt tctctctcct ggcgattaag
      901 ttgattctca aaatggttct gcaacgtcga taacacagtc gccatgatcc agtagatgag
      961 cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc gaggtaatca actgcgcctg
     1021 acggaacagc tccggcacct ggattgtcgc ggccagcgac gtatctttta ccagactgat
     1081 aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa tcgcgcgacg
     1141 catggtctgc cacggcgtca taccaatact ggcagcggct tcccactgac ctttatcaat
     1201 agaagaaatg gcggcgcgca gcgtttcggc ggcataggcg gcagtgttca gcgacagacc
     1261 aatcattgct gacggaatgg gatctaattc gataccaaac tgcggcaggc cgtaatagat
     1321 cataaacagt tgtgcgatga gcggcgtacc acgaaaaata gagatataaa aacgcgccag
     1381 ccagcgcacg ggccagattg gcgacaggcg catcagcgcg agaataaacc cgagtagtaa
     1441 gccaaaaaac atgccgccga tactgagttg cagggtatac ccagcccctt tcaacaggaa
     1501 cggcaaggaa tcaataacca gttgtatact ttcttgcatg agcgttcccg gagctaaacg
     1561 tggggatgat aggcgaacag cgcaggcgcg ccgccggtat gaataaacag aatcggccct
     1621 tcatctttga agcgtttctg actgataccg tcaatcagcc ccgccatcgc ttttccggta
     1681 tacacaggat caagcagaat gccttccaga cgcgccagca atttcactgc ttccatgcct
     1741 tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat cccagagtaa aatttccgct
     1801 gatgcggtca gctccagttc tttcgcaatc gcctgttgta ggttaaccac tttcggcaat
     1861 tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt cgctttcagg catcaggtgt
     1921 tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc ccgatgcgac taccaccgac
     1981 gaaatattaa ccgccccttc acactgttgc gcgatttcca gcgcactctc cacataacct
     2041 agcgcgccaa gagcattaga accgccaacc ggaatgacat acgggcgaaa gccttgtgct
     2101 tcgactcgcg tcgccagttc ttccagttgg gcattgggat cggtcagtgc gtcgcacatt
     2161 tcaatctggg tattgaacag atccagcaac aaacgattgc cgttggttaa atagttttct
     2221 gcggttgtgc caataggatt ttccagcagc gccacgcagt gcagaccgag tttcgccgcg
     2281 actgcggcag tctggcgcac atggttagac tggatcgcgc cggcagtaat cagcgtatcg
     2341 gcaccttcgc gcagggcatc tgccgcgaga aattccagct tacgtaattt attgccgccc
     2401 attgccatgg gggtgacctc atcccgtttg atgaaaattt cccgtccaag ataatcagaa
     2461 aagcgcggca gatattcgag cggcgttggc gcgccgataa actccaaccg tggaaaacgg
     2521 gttaaattat gcagtggcat aacagcctcc gatgtgtgtt gttgtgattt tcttattatg
     2581 cacgctgaaa acgcgtaaat aaaaaaggcg ctagtgaaag cgcccttttt tgtcattatg
     2641 ctgattattt ggtcacatca gcaccaaacc atttttcgga aagagcttgc agagtgccgt
     2701 ctttttgcat ttccgcaatt gcatcattca ccgctttcag caggtcttca tttcctttac
     2761 gcagcgccac gccagactcc tgacgggaga atgcttcacc ggttactgcc agcgtatcgt
     2821 tggttttctt caccagatcc agcgccgcca gacgatcaac gaggatcgca tcgatacgcc
     2881 ctacgcgcag atcctgatat ttggtcgggt catcatcata ggtacgcaca tcgacaccct
     2941 gaacattctg ccgcagccac tcttcatagt tggtgcccag accgacaccc acttttttgc
     3001 ctttcagatc agcagccgtt ttaatggtgc cttcgttacc ttttttcacc agcgcctgaa
     3061 taccggaaat ggtgtaaggg gtagagaaat cgtatttttt cttgcgctcg tcagaaatgg
     3121 tgacctgatt aatcaccaca tcaatgcgtt tagagtccag cgacgccagc ataccgtccc
     3181 atttggtcgg ttttagtgac gcctcaacgc caagatgttt tgccagctgt tgggcaaatt
     3241 ccacttcaaa accggttaat ttgccgtcat ccccctgaaa actgaacggc ggataagtgc
     3301 cttccagccc taccagcagc gtgccgctct ctttgacttt attaagcaga ccttcatctg
     3361 caaaactttt aacgctcatg cccgcaacca gcgccacggc catcacaccc atcaatgcct
     3421 gacgtcccag atgtgctaat ttcatattca ccccgaatgt tgttatgtct gtttgcagtg
     3481 tagagccatt gtttgtaaac acaaaaacaa ctccgctaca tcttattctt atttaatata
     3541 tatcagaagc aggcaggctg gaggatttct gcacaagtcc ggtacaggtt tggcgctgat
     3601 agtgttgata cttccgtaat gcgatgcggt aattgttggt gctggtggtg ggcagccacg
     3661 gggcgaggat ttcatcaagg aaaccgtcct gcagctgatc gagggaaata ttttgctcat
     3721 gtagatgatt acccaaacgg cgcagacgaa cgacatattc acgcaccgta cctgggctca
     3781 tttcagtttg ctcaaaaaga aatttcttaa agccgataat atcgaaaaaa tcactctgcg
     3841 ttttgcaatg taaatcaccg caaaatcggc acaatgccgc ccatgattgt tgttccgttt
     3901 gccagccttt ttcatcaagc agcgtgtcca ggcgggaaat ctcgatttta ttcactattt
     3961 tgccgtcgcg aactaaggta atgcgatcga gtaatttacg gcaatgcgcg caatgggtct
     4021 ggctgtgttt aaagtcttta agatagcggc ttaatggccg tcttttcagg tgctgcacca
     4081 tcattaagaa ctcctggtag tcaaagttaa agtgcggcat ttactgacgt tataacttac
     4141 ccagtttagt gcgtaaccgt ttaatggcct ggctgtgtaa ctgactgacc cgcgattccc
     4201 cgacctccag caccgcgcca atctctttga gattcagctc ttcctggtaa tagagggtta
     4261 ataccagttt ttcgcgctcc ggcaacgttt cgatggcttc catcacccgc tggcgcagat
     4321 tactgtccag cagttgttgt agcgggtttt ctcgctgatg atcatcagta accagttcga
     4381 tgctatcgcc gtgctcttca cgccactcat cgtaggagaa gagctggctg ttattggtgt
     4441 cgagcagcat ttgacgataa tcggcaatat cgatccctaa acgttccgct acctcagttt
     4501 ccgtggcgtt gcggccaagt tcctgctcca gttgccctat tgcctgtgcc acttcacgcg
     4561 cgttgcgtcg cacgctgcgc ggcacccagt cacggctgcg aagttcatcc agcatagcgc
     4621 cacggatacg ctacactgcg taagttgtaa atgccgttcc ttgtagggcg tcatagcgtt
     4681 cgacagcatt aagtaaccca atgccgcccg cctgtagcag atcgtcaagt tccacgctcg
     4741 cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac cagcgggaca taacgctgcc
     4801 acagcgagtg tttatccatt acaccttcag cggtatagag tgaattcacg ataaacagcc
     4861 ctgcgttata tgagttatcg gcatgattat ccgtttctgc agggttttta atcggacgat
     4921 tagtgggtga aatgaggggt tatttggggg ttacgcaaaa agccgaatgt aaaaaacccc
     4981 gccggtggcg gggtttgaga ggcattcaga cgattaaccc tgcagcagag acagaacctg
     5041 ctgcggtacc tggttggctt ttgccagcac ggagttaccg gcctgctgga taatctgcgc
     5101 tttcgacatg ttggacactt cggtcgcata gtcggcgtcc tgaatacggg actgcgcttc
     5161 agacaggttg gtagtggtgt tgttcaggtt ggtgactgcg gaatccaaac ggttttgcac
     5221 cgcacccagg gaggagcgga atttgtcgat ggagctgatg gcttcatcca gagctttcag
     5281 gggatcggct gtcgtagctg cttttgtttc agcatctgtc gtcaatttac catcagcatc
     5341 tttataaact gccttaccgc tgccgttagt cactgaacca tcatcctgta ggtaaaaatc
     5401 tacagtatca gatgtggtca acgcctcgtt gttatcaaca taaaccgaat caacaccttt
     5461 tgttacagcg ccagttgtgc cgttgacgct ataacctgca gtagtgaaat caacattgtt
     5521 tcccgaggtt gatacagctt tatcgaaagc tgcctgatca acttttgcac ctgttactga
     5581 ataagtaata gtgtccggcg tagtactatt accggtaaag gagatagagg taccgccaat
     5641 gtcaatggtt gtcgctttgg tattattggc tgcaagtgtg gacagactag cctcagtcaa
     5701 agaagcacca gagccgtttt tcgttaagcg cccagttgta tcaatgtaga gtttatctcc
     5761 attgcttgcc gtaattttac catcactgct gatctgaaca tcctgtgcag aaccatcgat
     5821 agtaatagtg cccttagcgg tatcaccaac tcccggggtc aaatatttct gaacatcggc
     5881 agctgaagcc gttgtggtat caaaagagta tgacttactt gcactgtcat acttgtaatt
     5941 cgttgcacta gccgccgttc cgaagccgtt attaatggtt gcctgaacag tatcaccatc
     6001 actaactcca gccagcaaat cagacgcttt agcagcatcg taacccgcac tcacagtata
     6061 tttgttgccg accactgttg cattagcagc taccaagtca gctttagatg cagcagtatt
     6121 agccacagcc ccgccaccat tcacattaaa cccattcagc cccagcgtat cagagtcaat
     6181 tttcttcaga tcaatagtga tagtctggcc gtcattcgca ccaacctgaa ttttcatcga
     6241 accgtctttt gccagtacgt tcacgccgtt gaactgggtc tgaccggata cgcggtcaat
     6301 ttcgtcgaga cgggatttga tttcgtcctg aatggagtcc agatccgaat cagagttagt
     6361 cccggtagaa gcctgaaccg tcagctcacg gatacgctgt aagttgttgt tgatttcgga
     6421 cagcgcgcct tcagtggtct gtgcaacaga aataccgtcg ttggcgttac gtgcagcctg
     6481 agtcaggcct ttaatgttag aagtaaaacg gttagcaatc gcctgacccg cggcgtcatc
     6541 cttcgcgctg ttaatacgca agccagaaga cagacgctcg atagaactcg acagcgcaga
     6601 ctggttcttg ttgatattat tttgagtgat cagcgagagg ctgttggtat taatgacttg
     6661 tgccatgatt cgttatccta tattgcaagt cgttgattac gttttgggtt tccacccgtc
     6721 ggctcaatcg ccgtcaaccc tgttatcgtc tgtcgtaaaa caacctttag aatttttttc
     6781 acaaacagcc attttttgtt agtcgacgaa atactctttt ctctgcccct tattcccgct
     6841 attaaaaaaa acaattaaac gtaaactttg cgcaattcag gccgataacc ccggtattcg
     6901 ttttacgtgt cgaaagataa aaggaaatcg catggcaagt atttcatcgc tgggagtcgg
     6961 gtcaggtctg gatttaagtt caatccttga tagcctcacc gccgcgcaaa aagcgacgct
     7021 aacccctatt tcaaatcagc aatcgtcgtt taccgctaaa cttagcgcct acggtacgct
     7081 gaaaagcgcg ctgacgactt tccagaccgc caatactgca ttgtctaaag ccgatctttt
     7141 ttccgccacc agcaccacca gcagcaccac cgcgttcagt gccaccaccg cgggtaacgc
     7201 catcgccggg aaatacacca tcagcgtcac ccatctggcg caggcgcaaa ccctgaccac
     7261 gcgcaccacc agagatgata cgaaaacggc gatcgccacc agcgacagca aactcaccat
     7321 tcaacaaggc gacgacaaag atccgattac cattgatatc agcgcggcta actcatcgtt
     7381 aagcgggatc cgtgatgcca tcaacaacgc aaaagcgggc gtaagtgcga gcatcattaa
     7441 cgtgggtaac ggtgaatatc gtctgtcagt cacatcaaat gacaccggcc ttgataatgc
     7501 gatgacactc tcggtcagcg gtgatgatgc gctacaaagt tttatgggct atgacgccag
     7561 tgccagcagc aacggtatgg aggtctcggt cgccgcccag aatgcgcagc tgacggtcaa
     7621 caacgtcgcc atcgagaaca gcagcaacac tatcagcgac gcgctggaaa acatcactct
     7681 gaacctgaac gatgtcacca cgggcaacca gacgctaacc atcactcagg acacctccaa
     7741 agtgcaaacg gcgattaaag actgggtgaa tgcctataac tcgctaatag ataccttcag
     7801 cagcctgacc aaatacaccg ccgtagatgc gggagctgat agccagagtt ctagcaatgg
     7861 cgcactgctc ggcgactcca cgctgcggac gattcagacg cagttgaagt cgatgctgag
     7921 taataccgtc agttcttcca gctataaaac gctggcgcag attggtatca cgaccgatcc
     7981 cagcgatggc aaactggaac tggatgccga caaactcacc gctgcactga aaaaagatgc
     8041 cagcggcgta ggtgcattga ttgttggcga tggtaaaaaa accggcatca caaccaccat
     8101 cggcagcaac ctgaccagtt ggctttcgac aacgggcatt attaaagccg ctaccgatgg
     8161 cgttagtaaa accctgaata agttaactaa agactacaac gccgccagcg atcgcattga
     8221 tgcgcaggtc gcgcgctaca aagaacaatt tacccaactg gacgttttaa tgacctcgtt
     8281 aaacagcacc agcagctact taacgcagca gttcgaaaac aacagtaatt ccaagtaatc
     8341 attattcatc gggagacagg tcatgtacgc ggcaaaaggc acccaggcct atgcacaaat
     8401 tggcgtcgaa agcaccgtaa tgagcgccag ccaacagcag ttggtcacca tgctatttga
     8461 tggagtgctg agcgcactgg ttagagcgcg actgtttatg caggacaaca atcagcaagg
     8521 caaaggcgtc tctttgtcaa aagcgatcaa catcattgag aacggactgc gggtgagtct
     8581 tgatgaagag agcaaagacg aattaaccca aaacttaatt gctctttata gctatatggt
     8641 caggcgtttg ctgcaagcca atttacgcaa cgatgtctca gcagtcgaag aggtggaagc
     8701 attaatgcgt aatattgccg atgcctggaa agagtcttta ctctcccctt cttcgattca
     8761 ggacccagtc tgatgaacaa tgcaccgcat ttgtatttcg cctggcaaca actcgtcgaa
     8821 aaaagccagc tcatgttacg cctggcaacg gaagaacaat gggacgaact catcgctagc
     8881 gaaatggcgt atgtgaatgc ggtgcaggag atcgcacacc tgactgaaga ggttgccccg
     8941 tccactacga tgcaggagca gctccgcccg atgctgcacc tgattctcga caacgaaagc
     9001 aaggtaaagc agttattaca gattcggatg gatgaactgg cgaaactggt cggtcagtca
     9061 tcggtgcaaa aatcggtgtt aagtgcctat ggcgatcagg gcggctttgt gctggctccg
     9121 caggataacc tcttttgaat cttaatgagc cgatggctcg cgaataatcc gattacggct
     9181 acgcttctaa tattcccctt gaatggagtc gaagaatgcg taatcccacg ctgttacaat
     9241 gttttcactg gtattacccg gaaggcggta agctctggcc tgaactggcc gagcgcgcag
     9301 acggttttaa tgatattggt atcaatatgg tctggttgcc gcccgcctat aaaggcgcat
     9361 cgggcggcta ttcggtcggc tacgactcct atgatttatt tgatctaggt gagtttgatc
     9421 agaaaggcag catccctact aaatatggtg ataaagcaca actgctggcc gccattgacg
     9481 ccctgaaacg taatgacatt gcggtgctgc tggacgtggt agtcaaccac aaaatgggcg
     9541 cggatgaaaa agaagctatt cgcgtgcagc gtgtaaatgc tgatgaccgt acgcaaattg
     9601 acgaagaaat cattgagtgt gaaggctgga cgcgttacac cttccccgct cgtgccgggc
     9661 aatactcgca gtttatctgg gatttcaaat gttttagcgg tatcgaccat atcgaacacc
     9721 ctgatgaaga tggcattttt aaaattgtta acgactatac cggagaaggc tggaacgatc
     9781 aggttgatga tgaactcggt aatttcgatt atctgatggg tgagaatatc gattttcgca
     9841 atcatgccgt gacggaagag attaaatact gggcgcgctg ggtgatggaa caaacgcaat
     9901 gcgacggctt tcgtcttgat gcggtcaaac atattccggc atggttttat aaagagtgga
     9961 tcgaacacgt acaggaagtt gcgccaaagc cgctgtttat tgtggctgaa tgctggtcgc
    10021 atgaagtcga taaactgcaa acgtatattg atcaggtgga aggcaaaacc atgctgtttg
    10081 atgcgccgct gcagatgaaa ttccatgaag catcgcgcat gggtcgcgac tacgacatga
    10141 cgcagatctt cactgggacg ctggtggaag ccgatccttt tcacgccgta accctcgttg
    10201 ccaaccacga cacccaaccg ttgcaagccc tcgaagcacc ggtcgaaccg tggtttaaac
    10261 cgctggcgta tgccttaatt ttgttgcggg aaaatggcgt tccttcggtg ttctatccgg
    10321 acctctacgg tgcacattac gaagatgtcg gtggtgacgg gcaaacctat ccgatagata
    10381 tgccaataat cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc gcccacggtg
    10441 tacagacatt atttttcgac catccgaact gcattgcctt tagccgcagt ggcaccgacg
    10501 aatatcccgg ctgcgtggtg gtcatgtcga acggagatga tggcgaaaaa accattcatc
    10561 tgggagagaa ttacggcaat aaaacctggc gtgattttct ggggaaccgg caagagagcg
    10621 tagtgacaga cgaaaacggc gaagcgacct tcttttgcaa cggcggcagc gtcagcgtgt
    10681 gggttatcga agaggtgatt taaatccatc cccggcggca agccggggag atttcattac
    10741 ggcagtggcg tcggtaacgg cgctttatcc agcgcggcgg cacactctgc cgtcgggcga
    10801 tccacacgct ccatcgtcat accatcgtac tcaatggtgt taccgtcacg ctcaacctcg
    10861 tacagctcac gttttacggt aacgttagtg agatcatcag acatcaatgt caacttaccc
    10921 ggcacggcaa tcccccgctg ccactgacgg caatccaagg tatcgccctc tttagtgaca
    10981 atcaggctgc ctatcgcttc cggactgacc agcgcacgtt gcggcccttt ggtctgccag
    11041 tagcccgcca gccagtccgg cgcgggggtt ttcacgacat tgttatagtt ttcgacttcg
    11101 gcacagcccg ccagcaacat gagcgcgcct gcaattgcga gttttttcat cattcgtcct
    11161 gcatgagaag aaaagaggat tgtggcatta aagctctgat gtcgccagcc tttccaggtt
    11221 cagagagaaa tttgatctaa ctattatttt tgtgaattat taccccgaaa ataaatttgc
    11281 atggtgtatg attcgcggct cttgtttcaa accttctgag ttcagaggct acattcatgt
    11341 catggcagca attcaaacac gcctggttga ttaaattctg ggcacccatc cctgcggtca
    11401 tcgcggcggg tattctctcc acttactatt ttggcattac tggcaccttt tgggctgtca
    11461 cgggtgaatt tacccgttgg ggcggtcagc tcctgcagct gtttggcgtc catgctgaag
    11521 agtggggtta ttttaaaatt atccatctgg aaggatcgcc attaacccgg atcgacggca
    11581 tgatgatcct cggtatgttt ggcggctgct ttgctgctgc gctgtgggcc aacaatgtca
    11641 aactacgcat gccacgcagc cgtatccgca ttatgcaggc catcattggc ggcattatcg
    11701 ccggttttgg cgcgcgtctg gcaatgggct gtaacctggc ggcgttcttt accggcattc
    11761 ctcagttctc gctgcatgcc tggttctttg ccatcgccac tgccattggt tcatggtttg
    11821 gcgcgcgctt taccctgctg cccatcttcc gtattcccgt gaaaatgcag aaagtttctg
    11881 ccgcctcacc gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt ctcgggatgc
    11941 tggtcttttt cggcatgctg ggctgggcgc tgctcacggc gatgaaccaa ccaaaactgg
    12001 ggctggcaat gctgtttggc gtcggctttg gtttactgat tgaacgtgcg caaatctgct
    12061 ttacttcggc gttccgcgat atgtggatca ccggacgtac ccatatggcg aaagcaatca
    12121 ttatcggtat ggcggtgagt gccatcggga tcttcagtta cgtacagtta ggcgttgaac
    12181 ctaaaatcat gtgggcggga ccaaatgcgg taattggtgg tttacttttt ggttttggca
    12241 tcgtgctggc aggcggctgc gaaaccggct ggatgtaccg cgcggtagaa ggccaggtgc
    12301 actactggtg ggtcggtctg ggcaacgtga tcggctcaac gattctggcg tattactggg
    12361 atgatttcgc tccggcgctg gccaccgact gggacaaaat caacctgctg aaaacttttg
    12421 gtccgatggg aggcctgctg gtgacatatt tgctgctgtt tgctgcgctg atgttgatta
    12481 tcggctggga aaaacgcttc ttccgccgtg aggcaccgca gactgctaag gagatcgcat
    12541 gaaaaatatc gttcctgatt accgccttga tatggtgggt gaaccctgcc cttatccggc
    12601 agtcgcaacc cttgaggcga tgccgcaatt aaaaaaaggt gagatcctgg aagtggtgag
    12661 cgactgtccg cagtcgatca ataatattcc actggatgcg cgtaatcacg gctacacggt
    12721 gctggatatt cagcaagacg ggccgaccat tcgttattta attcagaagt aatccctcat
    12781 tcccgctgtg gcccacagcg ggaacatcgt tacctctgac acatagattt tacccgcccc
    12841 ctctcctatt cttttcagta tgatctcagg aggttgctat gtgtggacgc tttgcccaat
    12901 cccaaacgcg tgaggattac cttgcgcttc tcgcggaaga tattgaacgc gatattccct
    12961 acgatcccga acccattggc agatacaatg tcgcgccggg aaccaaagtt cttctgctca
    13021 gtgaacgtga tgaacacctt catctggatc cggttttctg gggatatgct cccggatggt
    13081 gggataaacc gccgctgatt aacgcccgcg tagaaactgc ctcaaccagt cgcatgttta
    13141 aaccgctctg gcaacatggt cgggcaatct gttttgccga tggctggttt gagtggaaaa
    13201 aagaaggcga caaaaaaagc cttattttat ctatcgcgct gacggacaac ctgtttttat
    13261 ggccgcgata ggtagcatac cgtttgagcg tggtgacgaa gccgaaggat ttttgatcgt
    13321 cactgcggcg gcagatcaag gtctggtaga tattcatgac cgccgcccgc tggttctgtc
    13381 gccagaagct gcacgggaat ggatgcggca agacattggt ggtaaagaag cctcagaaat
    13441 cgctaccaga agctgtgtgc cagcaaacca gttcacctgg caccctgtgt cgcgcacggt
    13501 gggtaatgtc aaaaaccagg gcgcggagtt aattcaacct gtttgttgac atcctccacg
    13561 ccctgaatga cgaggacccc tgctacgttc aggctgtcgc ctgaatcatt tcggtgggtt
    13621 cctgcttcaa cgagcggcct gactgcacca tctctccaca ggcaagaacg gcgtgccccg
    13681 ccgctaaaat gttacgagct ccatttacat cggcgttcgc tgtatatcca catacctggc
    13741 atctgaattt actttgtgac aggcgatttt ctttcgctgt atgaccacag cacgcgcaac
    13801 gctggcttgt gtacgctggc ggaacagcaa gcacctgacc gccacgccag agctgcttgt
    13861 actcaagctg gcggcgcatt tcataccagc cctgatccag tatcgaacgg tttaaacctg
    13921 attttgcccg gacattgcgc cccggctggc tgaccgtacc cgctgctgac tttgacatgt
    13981 tgctgacctt caaatcctca atgactatca ttacgtggtt tttgctgacg gtcgttgtga
    14041 ctttgtgaag gtagtccctg cggatatttg cgatacagga atgcagtcgc tgtattttgc
    14101 gtttctgctt ctgccagttg ttgctgaatt tgaccttgcg gcttaactgt cgctgaagtc
    14161 tcgccagcgt cttctggttt ttctgaaaac tgtttacagg ctcaaagact gtgccatctg
    14221 acagcgaggc aagtttagcc acgccagcat ccagcccgac cattgatgct gaagggtgaa
    14281 ccggagttga tacttcactt tctgcctgaa tactgatgta ccacttaccg caggactggc
    14341 tgacagtgac atttttcaca acacccgtga cctgacggct gttccggtag cgcatccagc
    14401 caagttttgg cagaaaaata cggctgtttt cctgagcgag cttaacgccc tgcggatagc
    14461 ggaatgcatc attctgtcac cgctttttga atcaggggaa cgccgagcgc ttctggaaaa
    14521 agtttttgca ggcctgctca gggtctttca gtgactgttg caatggctgt gacggagcat
    14581 ctttaatcca ttgcgtttca gtggcatttt tccactcaac caatcaggaa gccattttcg
    14641 cgtaagggat gtctttgtta ccggccttat gattctcatt ctgacgtgcc agagtacgac
    14701 tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc acgcgcctgt tgaccaccgg
    14761 gtcttaaccg gaatttgaat acctgtagtc gtttcacagg gcaaattata ctttggtctg
    14821 tgaaaaaaac tgatatccga cgcggaaggc actgtgtttt cctgatgcat gctcaccggg
    14881 tctttgttac caagtaccga cgccagattt ttgaccatga cgcgacagaa aaactacgca
    14941 cttacttttc aaatgtatgt gcttattttg aagctgaact ggttgaaatg gatggcgaac
    15001 cagatcacgt ccatctgtta attaactatc cccccaaact ggcgatatcc agtctggtaa
    15061 acaaacagcc tcaaaggcgt atcgggtagg ttactgcgac gggatcgacc agatattgct
    15121 gccaggtatt accgcaaaga tgttttgtgg cgtcccggct attttgccag tagctgcgga
    15181 ggtgcgccaa tatccgtcat ccgccaatac attgaacaac agcaaacacc tggtcagatg
    15241 aaaaaacgag ccttatatcc ccgccctgaa ggacgggctt ttacggtgca ctggataagc
    15301 ctggagatta tctacacctg catggacatc attgtacgat gccccgtaca tgctccatca
    15361 catcctcaac atcaaagtat tagagccttg caaaccatta tgcgtctcag tttgtctcat
    15421 ctatcttacg caaaaaaaat agaactaata tcaagcggcg ctatattgcg tattaaataa
    15481 aacca
//
LOCUS       DAZSNO010000084        15399 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZSNO010000084 DAZSNO010000000
VERSION     DAZSNO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15399
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.084"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..74)
                     /locus_tag="ACHWN2_002547"
     CDS             complement(<1..74)
                     /locus_tag="ACHWN2_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HFR8932041.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="ACHWN2_002548"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="ACHWN2_002548"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR8932042.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="ACHWN2_002549"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="ACHWN2_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR8932043.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="ACHWN2_002550"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="ACHWN2_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR8932044.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="ACHWN2_002551"
     CDS             2704..2940
                     /locus_tag="ACHWN2_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR8932045.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="ACHWN2_002552"
     CDS             2943..3800
                     /locus_tag="ACHWN2_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR8932046.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="ACHWN2_002553"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="ACHWN2_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="ACHWN2_002554"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="ACHWN2_002554"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR8932047.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="ACHWN2_002555"
     CDS             complement(6415..7311)
                     /locus_tag="ACHWN2_002555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR8932048.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="ACHWN2_002556"
     CDS             complement(7360..7599)
                     /locus_tag="ACHWN2_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR8932049.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /gene="nemR"
                     /locus_tag="ACHWN2_002557"
     CDS             7702..8301
                     /gene="nemR"
                     /locus_tag="ACHWN2_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR8932050.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="ACHWN2_002558"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="ACHWN2_002558"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR8932051.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="ACHWN2_002559"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="ACHWN2_002559"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR8932052.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="ACHWN2_002560"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="ACHWN2_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR8932053.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..15224
                     /locus_tag="ACHWN2_002561"
                     /pseudo
     CDS             10766..15224
                     /locus_tag="ACHWN2_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15268..>15399)
                     /locus_tag="ACHWN2_002562"
     CDS             complement(15268..>15399)
                     /locus_tag="ACHWN2_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFR8932054.1"
                     /translation="ANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3534 a         4072 c         4049 g         3744 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccagct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg cagtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccaac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctaggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccacaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacagcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgttttaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatgga gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctgggctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    15361 cgctaaaatg ttacgagcgc catttacatc ggcgttcgc
//
LOCUS       DAZSNO010000085        15350 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZSNO010000085 DAZSNO010000000
VERSION     DAZSNO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15350
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.085"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            115..765
                     /gene="csgD"
                     /locus_tag="ACHWN2_002563"
     CDS             115..765
                     /gene="csgD"
                     /locus_tag="ACHWN2_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR8932055.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            770..1159
                     /gene="csgE"
                     /locus_tag="ACHWN2_002564"
     CDS             770..1159
                     /gene="csgE"
                     /locus_tag="ACHWN2_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR8932056.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1184..1600
                     /gene="csgF"
                     /locus_tag="ACHWN2_002565"
     CDS             1184..1600
                     /gene="csgF"
                     /locus_tag="ACHWN2_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR8932057.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1627..2402
                     /locus_tag="ACHWN2_002566"
                     /pseudo
     CDS             1627..2402
                     /locus_tag="ACHWN2_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(2466..2957)
                     /locus_tag="ACHWN2_002567"
     CDS             complement(2466..2957)
                     /locus_tag="ACHWN2_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR8932058.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3059..3613)
                     /gene="ycdY"
                     /locus_tag="ACHWN2_002568"
     CDS             complement(3059..3613)
                     /gene="ycdY"
                     /locus_tag="ACHWN2_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR8932059.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3637..4374)
                     /gene="ycdX"
                     /locus_tag="ACHWN2_002569"
     CDS             complement(3637..4374)
                     /gene="ycdX"
                     /locus_tag="ACHWN2_002569"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR8932060.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4429..5367)
                     /gene="ghrA"
                     /locus_tag="ACHWN2_002570"
                     /pseudo
     CDS             complement(4429..5367)
                     /gene="ghrA"
                     /locus_tag="ACHWN2_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            5602..5689
                     /locus_tag="ACHWN2_002571"
     tRNA            5602..5689
                     /locus_tag="ACHWN2_002571"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5636..5638,aa:Ser,seq:gga)
     gene            complement(5839..6120)
                     /locus_tag="ACHWN2_002572"
                     /pseudo
     CDS             complement(5839..6120)
                     /locus_tag="ACHWN2_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(6402..7406)
                     /locus_tag="ACHWN2_002573"
     CDS             complement(6402..7406)
                     /locus_tag="ACHWN2_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HFR8932061.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(7478..8038)
                     /locus_tag="ACHWN2_002574"
                     /pseudo
     CDS             complement(7478..8038)
                     /locus_tag="ACHWN2_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(8123..8320)
                     /locus_tag="ACHWN2_002575"
     CDS             complement(8123..8320)
                     /locus_tag="ACHWN2_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFR8932062.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(8332..8823)
                     /locus_tag="ACHWN2_002576"
     CDS             complement(8332..8823)
                     /locus_tag="ACHWN2_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFR8932063.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(8820..9194)
                     /locus_tag="ACHWN2_002577"
     CDS             complement(8820..9194)
                     /locus_tag="ACHWN2_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFR8932064.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(9241..9615)
                     /locus_tag="ACHWN2_002578"
     CDS             complement(9241..9615)
                     /locus_tag="ACHWN2_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFR8932065.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(9778..9999)
                     /locus_tag="ACHWN2_002579"
     CDS             complement(9778..9999)
                     /locus_tag="ACHWN2_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFR8932066.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10062..10538)
                     /locus_tag="ACHWN2_002580"
     CDS             complement(10062..10538)
                     /locus_tag="ACHWN2_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFR8932067.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(10554..11027)
                     /locus_tag="ACHWN2_002581"
     CDS             complement(10554..11027)
                     /locus_tag="ACHWN2_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR8932068.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(11369..12187)
                     /locus_tag="ACHWN2_002582"
     CDS             complement(11369..12187)
                     /locus_tag="ACHWN2_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR8932069.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(12305..12500)
                     /locus_tag="ACHWN2_002583"
                     /pseudo
     CDS             complement(12305..12500)
                     /locus_tag="ACHWN2_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            complement(12571..12789)
                     /locus_tag="ACHWN2_002584"
                     /pseudo
     CDS             complement(12571..12789)
                     /locus_tag="ACHWN2_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            12984..14525
                     /gene="istA"
                     /locus_tag="ACHWN2_002585"
     CDS             12984..14525
                     /gene="istA"
                     /locus_tag="ACHWN2_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HFR8932070.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            14540..15286
                     /gene="istB"
                     /locus_tag="ACHWN2_002586"
     CDS             14540..15286
                     /gene="istB"
                     /locus_tag="ACHWN2_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HFR8932071.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT         3940 a         3793 c         3793 g         3824 t
ORIGIN      
        1 tttagggctg atttattact acacacagca gtgcaacatc tgtcagtact tctggtgctt
       61 ctattttaga ggcagctgtc aggtgtgcga tcaataaaaa agtggggttt catcatgttt
      121 aatgaagtcc atagtattca tggtcataca ttattgttga tcactaaacc ttctttgcag
      181 gcgacagctc tcttgcagca ccttaaacaa tcgctagcaa ttacaggaaa attacataat
      241 attcaacgtt ctctggacga tatctcttca ggctctatta ttcttctgga tatgatggaa
      301 gcggataaaa agcttatcca ttattggcag gatactttga gcaggaaaaa caacaatatc
      361 aaaatattgt tgctaaatac gcctgaagat tacccgtacc gcgacattga aaactggcct
      421 catatcaacg gcgtttttta tgccatggag gatcaagaac gtgttgtcaa tgggttgcaa
      481 ggcgtcctgc gtggcgaatg ctactttacg caaaagcttg ccagttacct gattacacat
      541 tctggtaact atcgttataa cagcacggaa tcagccctcc ttactcatcg ggaaaaagag
      601 atcctgaata agctgcgtat cggcgcgtct aataacgaga tcgctcgttc gttgttcatc
      661 agcgaaaata cggttaaaac gcatctttat aatcttttca agaagatagc cgtaaaaaac
      721 cggacacaag cggtttcctg ggcaaacgat aacctcaggc gataaagcca tgaaacgtta
      781 tttacgctgg attgtggcgg cagaatttct gttcgccgca gggaatcttc acgccgttga
      841 ggtagaagtc ccgggattgt taactgacca tactgtttca tctattggcc atgattttta
      901 ccgagccttt agtgataaat gggaaagtga ctatacgggt aacttaacga ttaatgaaag
      961 gcccagtgca cgatggggaa gctggatcac aataacggtc aatcaggatg ttattttcca
     1021 gactttttta ttcccgttga aaagagactc cgagaaaact gtcgtctttg cactgattca
     1081 aactgaagaa gcactaaatc gtcgccagat aaatcaggcg ttattaagta cggacgattt
     1141 ggcgcatgat gaattctaaa taaaaaattg ttcggaggct gcaatgcgtg tcaaacatgc
     1201 agtagttcta ctcatgctta tttcgccatt aagttgggct ggaaccatga ctttccagtt
     1261 ccgtaatcca aactttggtg gtaacccaaa taatggcgct tttttattaa atagcgctca
     1321 ggcccaaaac tcttataaag atccgagcta taacgatgac tttggtattg aaacaccctc
     1381 agcgttagat aactttactc aggccatcca gtcacaaatt ttaggtgggc tactgtcgaa
     1441 tattaatacc ggtaaaccgg gccgcatggt gaccaacgat tatattgttg atattgctaa
     1501 ccgcgatggt caattgcagt tgaacgtgac agatcgtaaa accggacaaa cctcgaccat
     1561 ccaggtttcg ggtttacaaa ataactcaac cgatttttaa gccccagctt cataaggaaa
     1621 ataatcatgc agcgcttatt tcttttggtt gccgtcatgt tactgagcgg atgcttaacc
     1681 gccccgccta aagaagccgc cagaccgaca ttaatgcctc gtgctcagag ctacaaagat
     1741 ttgacccatc tgccagcgcc gacgggtaag atctttgttt cggtatacaa cattcaggac
     1801 gaaaccgggc aatttaaacc ctacccggca agtaacttct ccactgctgt tccgcaaagc
     1861 gccagggctt acaaaacctg cttaacgagc gcaaggttat tcgtgcggca caagaaaacg
     1921 gcacggttgc cattaataac cgaatcccgc tgcaatcttt aacggcggca aatattatgg
     1981 ttgaaggttc gattatcggt tatgaaagca acgttaaatc gggcggtgtt ggggcaagat
     2041 attttgggat cggtgcagac acgcaatacc agctcgatca gattgccgtg aacctgcgtg
     2101 tcgtcaatgt gagtaccggt gagatcctct cctcggtgaa caccagtaag acgatacttt
     2161 cctatgaagt tcaggccggg gttttccgct ttattgacta ccagcgcttg ctggaagggg
     2221 aagtgggtta cacctcaaac gaacccgtta tgctgtgtct gatgtcggct atcgaaacag
     2281 gggtcatttt cctgattaat gatggtatcg accgtggtct gtgggatttg taaaataaag
     2341 cagaacggca gaatgacatt ctggtgaaat accgccatat gtcggttcca ccggaatcct
     2401 gacagataaa aatgccggaa ttaataatcc ggcatttact gtttaagcga aagcgtgagt
     2461 gcccctcacg ctttattttt gatttcctgc tcacggtgag ccgtctttgc actacgcgcc
     2521 gccagccaca ggccgctgtt tttcattgcg taaccaaata ccagcccaag cgccagcgaa
     2581 ggtaacacca gtttccagtc accctgcccg gcaaacgtcg cgcatgcgcc tataaacgtc
     2641 cccggaacaa atgaaagcag cagctgtttg gcctgaatac acatcagaaa cgcgacaata
     2701 ccggttatga cataaccgag aatttccaga tgtggtgcca gtgcactacc gtaaataatg
     2761 accatcgccc acaccacgcc actaagcagg gttgcagcgg agatcgccag ccctttcagc
     2821 ccaccttgcg ggcaagcaaa gtaggccgta cagcccagga agcccgccca gcttagtaag
     2881 cccagggaaa cagccaccca tccccagata ccggagagaa tgcctgttgt gattgcaata
     2941 gagagaagta tgttcatgcc gcgcatgata gcagaaaacg cggccatgaa tgagatcgag
     3001 cacacatttt atgcaacaac aaattatctg ttgcagttat gagtgacgat gatcacattt
     3061 actcttcaga atcttcttcc agctcgtccc acattgcact aatggcatca cgggttagcg
     3121 gtgccatggt gcgccagaaa ggcgtggttg catgggcctc cactttgccg aggaacgcac
     3181 cacaccaggg taacagatac tcactgaaca gtgtttccag tgcttcgctc tcatcttccg
     3241 ttgactgatc ttccagccag gaagccgcaa gcagcaatgt gccgatgtga tcggctggcg
     3301 tatccgccag tagcatccct cgttcggaaa gaaaagcgcg cacttccgct tccgttgcgt
     3361 cctcaaccca tgcgctacga tatggcggca cggcacattc atcgccgata aacaacgcat
     3421 tgtaatcggc agagacttgc gtcatatcac agcttttctg taaacgcgtc agtaactcat
     3481 cctgctccag tggccagttc gcagccagtt tcccctcacg aatcagagta aacagcggca
     3541 ccagtaaagg atcttgcggt tggcggtaat agagcgaacc cagcacacga cagaggatag
     3601 aaaactcgtt cattaatata tttccattac tagtaattaa aggtctgcaa attccgcaat
     3661 cggtgccata ccgcgagatt caaggaagtt cagtaagcgg cgcggagaaa cattcaaaat
     3721 gcgctctagc ggaaaatcta ccgcgtcgag gattttaaga cactcttcaa attcccccat
     3781 ggtaaacgcg gtgtgagaat ccgagcctaa tgccacccaa cctccagcat cacgtaccgc
     3841 tgcagctacc gcacgacagt tgtcttcact gcccttacgt gagtgtaaaa atgaggaatt
     3901 attgatttcc agcgccacct gatgtttcgc ggctgcttca gcaacggctt taacatctat
     3961 ttcatatttc ggatttccgg gatggcttat tatatgcaca ttgccgctgg cgatagtgga
     4021 gatcatcgct tgtgtattgg tcgctttgtc atgtggcgca aaaaccggct catgaaaacc
     4081 ggcaataatc agatccagcg agtcaaacat tttaccgctg cagtcaattt caccatcaac
     4141 atttttaatg ttagcttcaa tgccgcgcag gatccctacc ccatcaacca ctcgcggcca
     4201 gatacgcatg ttaatgaagt gccagtgatg cggcgcatct tccatatccg ggccatgatc
     4261 ggtgatcgca aaaagtttaa tgcccttttg tttggcctgg gcaatgtaat cactcaatgt
     4321 gctatatgca tgtgtgctgg caacggtatg catatgaagg tcgacgggat acataactct
     4381 ctcctgtatt caattcccgc caaggatagc aggaatcctg atgctttatt agtagccacg
     4441 tgcgcggtcg acttgcccgc agaccctctc ccctttttcg agctgggcaa tagtgcgaga
     4501 aatgtactcc acagcttcag cgggacgggt aatcgcggcg acatgtggtg ttatcgtcac
     4561 gcgtggatgt tgccagagcg gactttcagg cggtaagggt tcacgattaa aaacatccag
     4621 cattgcgcct ttaactttcc cgctatccag cgccgcgagc aggtcatctt ccacaacatg
     4681 aacaccacgc gccaggttga ggagatacgc gccatcaggt aatttttcga gtaattgttg
     4741 attaataatg ccgacggttt caggggtatt cggtaacaaa ttaatcaata cccgacattg
     4801 gctcagaaat gcagacagtt cttcccgtcc ggcaaagctt tgcacgccag gccacgattt
     4861 acgggttcga ctccagcaac gcagcggaaa gcgctaggtt tgcagactct gagcaacttt
     4921 acagcccagt acgcctgcgc ccaaaatgcc gatggtaaaa tcttcccgat gatattcagg
     4981 cagcggttgc caatgcgaac tattttgctg gatgcgataa tcgtcaaaac gtcgaaacca
     5041 atgcagcacc tgactgacag catattcctg catttgctcg cccataccgg tatcttccag
     5101 gcgaaaaagt ggaacagaag ggttcagcat ttcagggtgt gcctgtagct tgctcaaaat
     5161 agaatcaaca ccggccccga gtgcgaacac cgctttaaga tcgcgccctg ccagcatttc
     5221 aacaggagga tgccagacta aaacataatc agcagagtca ttatctccgc ttttccatgc
     5281 cctgactctt gcctgaggaa tagctttgcg cagtgcctca atccaccatt gggtatcgaa
     5341 cgttgggtga taaaagatga tatccatact gactcccgaa aagcgttgtg cgaaatttat
     5401 tcgcacttat cgttatgatc tacaaaggcc accagcataa caaatccgtg gtcggtggca
     5461 aaaaaagcag atttcgctta ttaaaaccac acattgattg aatgtttgaa taaacgcgcg
     5521 attttttcaa aaagtttgtt gacctcaggt catgatttcc ctaaattagc gcccgttcca
     5581 gcaagacagg aacgacaatt tggtgaggtg tccgagtggc tgaaggagca cgcctggaaa
     5641 gtgtgtatac ggcaacgtat cgggggttcg aatccccccc tcaccgccag attataagtg
     5701 catgttattg tttaacatgc gcttttttat ttttccttcc agacagtatg ccatcgggta
     5761 tgccatctga aaccggttta tatgccccgc gatcatatct tatcggacaa tttccgatct
     5821 ttgagcatac tggcaaagct atactgccag catatcagga tagtgttttg cgataatatc
     5881 attcagtctg tcaaccagtt ctggttttct gaacgtgata tgcgcggagc ccttcttaaa
     5941 gtatctgatg ctgaacatct catcttcata gctctcttta ccctgaacag actgaatgtg
     6001 atcatccaga cgaagggtga tgtttccccg gttgtcgggg attggtttac cgctgaacag
     6061 gtgcaacatt ctttccagat cggcgagtcg gtcagcctgt tgtccggtga taagatgaaa
     6121 tctatggtca cccccgtttt tgcaacactg atttttgatt aatggttggc ttgcttaaat
     6181 ctatccggcg tctctgtggg caaggatgcc cgcgccccga tgagttccgc acctgataat
     6241 cctaaaaatc ccctcggctt cagtgagcca tttcatctgt taggttttta atcaggtcga
     6301 tttgccgttc atgtcatcaa taatcagtct tcgcaaaacc agaaaggtaa cttgtatctt
     6361 attgtttaaa ataaactaat tgagttatat cagcatctat atcaggcctg atattctttt
     6421 ttctgagtag tgattgccca ggcaattcga gccagtttat ttgccagagc gcaggtaacc
     6481 acattcgaat gcttcttgtt gagttgctcc cgaacccact cagcaagttt tccatgcttg
     6541 cgttcaagtc gcattatgaa tgagcgggca cactgaacca gtaaccggcg taaattttta
     6601 tcacctcgct tactgatacc caacagagtc gattttcctc cagtgctata ctgccgggga
     6661 acaagtcctg tggaagctgc aaaatccctg ctgcatgaga actgtttccc gtcaccaagt
     6721 tgcgacgata gtaagctggc agtaatcgga ccaacaccag gaattgtcat aatgcgctga
     6781 gcggtatcat cagcgttgat tgactgactt aattctgatt ccagctctgc tatttgctca
     6841 acaaggtaaa agtagtgggt atgcaacctc accaaaaggc ggctcagata ttcggggatt
     6901 tcgtgttcag ccaacacgag agacaaccgt tttattacag catcacctat cggcaggctg
     6961 attccaaatt ccagcaagaa accgtggatc tggttggttg ttttcacctt atctctgatg
     7021 agcgattctc tgacccggtg taacgccctc atggcctgct gggtctcagt tcttggctgg
     7081 acaaaacgca ttgatggtcg tgaagccgct tcgcatatgg cttctgcatc aacaaaatca
     7141 ttcttattac ttttgacaaa cgggcgaacg aactgtggag aaatcagctt agtttcatgc
     7201 ccaaaatcag agatacagcg agccataaag tgtgcacccg cacaggcttc cattacaaca
     7261 actgagggcg gacatgtcgc cagaaactgc atcagtttag tacgggtaaa ttttttacgc
     7321 agaagagcct taccagactt gtcttgacaa tgaatgtgga aggagtgctt tccgagatcg
     7381 ataccaataa gtgtgatgtt ctgcatgatg gtctctccgt taaagggatt taccttaaca
     7441 gcgtactgct gttaaggtgg gggtgaccat atcattatcc ccaccggtcc catctcacca
     7501 gattgttgac gataatttta ctgccaaatt tacaggggca attggttttg taattccagt
     7561 tcagccctct gaagacgttg atcacccctc gctcaaacac ctcatcctta ttctggtgca
     7621 gttgttcaaa tgtgctcaga atgttcgctt cgctgatttc cggaaaatta tcgtactcca
     7681 gtgaccggta ccatgtatca cgggcctgcg tatccattaa tgacagcatc ccggaacgtt
     7741 gcatcaggtc acgccagatt tcgcgatcaa gattccgggt aatggctttc attgctgttt
     7801 cgggtttctc catcagccag cagccgcagc gaaaatcctg tttcatggcc cagtccgggg
     7861 cagttttacc tccgatgctt tccgtcagca ccgagatttc agccagttgt cggataagtg
     7921 tgtcgatttg caacagtgta ctctttcgta cagccagaat gtggcgaatg ctggttgagc
     7981 agataacgtc cgtatgcccg ttgaccactt ccggacggga gattgtgata tctgacataa
     8041 tcgatattcc tttttgcaga taaatgaaaa cgccagccct gtgcaggctg gcgtcagagg
     8101 aatgaagtct ggtgagtgag cttcaccggt tctggtgcag aagctgcaga tgacgcaggg
     8161 tacggacatc ctcccgggcc tgttcgatac agggcaacag tgctgctgaa tccgttttat
     8221 cctcatcgtt gtcaaagata attcccgatc cgcagtcgat attgtcctgc agccacgcaa
     8281 tcagaatatc cagcgctgtt tccgtggtta atgatttcat gttgttcctg gttaccagtc
     8341 gaaagtgggt aaacccggca ggcattcagc gctggcatcc agatgaatga gactgacgcc
     8401 ataacgccgg atgagtgtga cgaccagacg acggaacgtt tttgacagcc ccaggcgttt
     8461 aagccgcaga accgggtatg accacgcatc ggtacgtaac agatatccgc tacctgtaaa
     8521 atgaatccat tctgattcac cgaagtcact ggtctggtgt gacagcgagt acaaccaggc
     8581 gttgtccgtt tccgtgatat gtgcggtact gcagcgtatg ccggtaaggg tagcaaatgg
     8641 tggcgtgcat tctgctgagg tctggtcaga ttcatcaaca acacgaaggg agtagccgtt
     8701 atcgcagacc atgttaatca gttcagcgag ctcaatacca tcgacgtcaa cgacaatgcg
     8761 ccctatgttc agtgcctgta cgttaacgct gtcggcttcc agtgtcaggg ccagtttcat
     8821 catttcgcct ccggatactt acccagggta atgttattta ccgttctgta attgtcgcgg
     8881 gtcatcaggc cggtcgcccg gcgagcccgg aggatatcaa tactgtttat taactgagag
     8941 cggggacagg tgctgaatcc cggctggtcg gtacgcacca gcgcgtattt ttccacgaga
     9001 aaattaactg catcacacag tgaaatgcct gcctcaatat gctgctcaat cacacgttca
     9061 tcgacaaacg gtgtgtcatt cagtgtgagg ccataatgct ggtccagcag tcggatgagc
     9121 agtgtctgcc agatggtgac gggagacggg cagcgagata cctcccgtac atgtgtgtcg
     9181 gataatgttt tcatcttaag ttctctgtaa ctggtagtgc taaaggtgag agcttcgggg
     9241 ttatgaggtg gttgcgggtg ctggtgtcgg ataaacagcc agataaacgt aaccacagga
     9301 gccgagggtg tcggcttcgc aggtcagccc ttttgcatac agcgtgacgg tatgctgata
     9361 tcggggattc agttcaccgc tggtgagcat gagttccagt tgtttcatca gcagcggaaa
     9421 ggcctggtcc agatggtatg catctgcgtc gctgaagcgg cctctgatac cggcgcggtc
     9481 ggcaaggtaa tacaggcggt taccctcttg caccagacga gcgccaaaac agggcctcac
     9541 tgtgcaggga agcccccacc aggggcggtc gtgattgtca tcgggatgag ttatcccgga
     9601 gagtttgtct gacacgataa aatcctctct gaaaagtggt taagaatggt ttaacgatga
     9661 caggtcagcc ggcgcgctga ccggtttcgg tgaagatgta tgcattgatg agaatggcct
     9721 catccacagc ggcgtctgag gtcatctggt cgtactcgtt tcccgggtga cggaacatca
     9781 gttcagggtg acactcatca gccggacata cgggccaaaa ctgtccttac ggcgttctgc
     9841 aaacacggca agaacgccgg gaatatcctg cacttcacga ccagtatacg cttcagcact
     9901 gccgtgccag cgatacttac cggtacagaa cggaaaaaga cgggatgcag gatgctgttg
     9961 gtgaatacgc atggcttcac cacgggtgat gattctcatg atgtgatacc tctgaagaca
    10021 gaagataaaa gtgaaaacag gtgtgatgtg gttgtgacgg gttaaagcag accatgttcg
    10081 gcaaaggaga aaacctggtt gccaccgact atcagatggt ccggcacccg gatatccacc
    10141 aggcccagtg cctgtaccag acgttcggtg ataagccggt ctgccttact gggtgtgact
    10201 tcaccggacg ggtgattgtg cgccagcacc acggcagcgg cattgtggta cagggcgcgt
    10261 ttaatcactt cccggggatg aacttccgta cggttgatgg tgccggtgaa gagggtttca
    10321 ccggcaatca gctgattctg gttgttcaga tacagcaccc ggaactcttc ccgctccagt
    10381 cccgccatgt tcagaatcag ccattcccgt gccgcacggg tggaggtgaa ggccacgccg
    10441 ggttcatgaa gatggcggtc cagggtttgc agggcccgca gaatgagact gcgctcgccg
    10501 ggcgtcatgt ttccgggcag aaaggaaagc tgctgcattg tccctgcctc cattcagtcg
    10561 atgatgcgca taatggcgct gcattccgga tgctgcaggg cgtaatcccg cagccggtaa
    10621 taatggaccg tcatggcata acactccgta cgacaggcat gatgactgta cgtcatcaga
    10681 caggcagcaa tgccggcggc ttccgggctc atttcagcgc ggttaccgtt catggcattg
    10741 aacagtaccc atgtttcgtc atcatcgtca tccggttcgg gtgccataaa tgccccgccg
    10801 ttgttcaggg tgtacagatt ccagatacca ccgcagtagt cttcacacag acggtccatc
    10861 cagccgaaga cacggggttc cagggtgacc cactgtggaa tgaggccaaa atgctgcggc
    10921 cagaagctga tgcgctgttc atcagggacg ggagtggcaa ccagttgttg caggccagtc
    10981 tccggtgcac aggccgaagc ggtggtgttc tgagacaatg ttttcatgta tgaaatcctt
    11041 gtaatgtgaa taaatgagag ggagtaaagg tcgggagctg gcaacatcac gttcatcact
    11101 gaccgtgatg acccggtaat atcagggatg aatgatattc ggctgaaatt ccggacagcc
    11161 gtacgcgtaa ccgttccggt cacagacgga tggccagatg ccattgcctg tatactcagt
    11221 ctgcacagac tgatttctcc gggacggtaa agcagacggg tacaccaggc gcacaggtca
    11281 tcatcagaaa tggaacgaca ccccggcagc gcctccgggg cgtcgggtga ttctggtgcc
    11341 tgaatcacgc cttcagggac atgctgcatc agcgcaggct ctccagcagc gtttctgcca
    11401 ttacccacaa tgcgcggttg agcttaatat cggtgtcgat gttgtgaatg gcacgggtgt
    11461 ggatacgttt tccttttgca ctgcggccgg aaatcccgcc tttcagcata ttctcctgga
    11521 tggtctgata tgcgctccac aggtctttac cgtaatcctc cctgcgtcgt ggtgtcagaa
    11581 tgtcagcggt ggtgactggc tgatgttcgt caccataacg gtaagtcagt gccgcctgtg
    11641 ccagcgcctg gcgtgccggt ggcggcagga ccagcgactg catggcatca cgcttttcct
    11701 caatccggtc aaaaaccccc accacttcgt aagccccttc gataactttc tccaccacat
    11761 ttccccggtg tggaacacgc acttccccca gagactgacc gcagacgcag ccattctggc
    11821 agacgaatct gaagtaaccc ggcagcatct ggtagctgga ggtaccgtca tgagagttga
    11881 gcagaataat ttcagggaca tgttctccgt ttatctctcc ggcccgccgc agacgcagca
    11941 tgtgttttgt gtatccccgg cggcccgggt cgcgcacgcg ggtctggcag gcgaagaatg
    12001 gctgaaaacc ttcctgctgc aggctttcca gtacggtgat ggtgggaatg tacgcatatc
    12061 gtttactgcg ggaagtatgc cggtcttcac cgaaaatact gggaacatag tgcatcagtt
    12121 cttcgtgtgt cagcggacgg tcacggcgta tctggttcgc ataaccaaaa cgactggcta
    12181 gtcgcataat ttgctcctta tcggtggtta cgatttactg gtgtaataaa tgaaaaagct
    12241 gcgtctcccg gagaagacgc ggcctgacag atgaaatgaa tgacgtttat tgtctgagaa
    12301 gcccttaact ggcgagctga gtattaagct gtgttccggc atcaccagcg caactgacct
    12361 tcagcattac ggataaccag ccgggaatat gttccctggt catcttcagt aaacacattg
    12421 cggtaagctg ttttgacggc aacagcctgt tcgcgtgaga aagggccttc gggcaataaa
    12481 cgtgatgtac aacccggcat atctgttgct ccctgaaagt aaaaagcccc ggtcatgatg
    12541 accggggcct gaaggagagt gacctgatta tcaaaaagtc acattcagcg tggcctgacc
    12601 gttataacct tcagcgctgc tgccgctgac gctgtgggca taaccggcct gaacgcccag
    12661 ggtgatattt tcccggacac gggcttccag tccggcctgc aggtccagtg acgtgccatt
    12721 ccgggacggt gagaacgtca tgttactgcc ggcggcggct gtccccatgc tcatgtcacc
    12781 ccgggagctg tgcgtatttt catgaaagga gatcactcaa taacttccat cgagatcggg
    12841 taataacatt tgaacagatc gctgaataac atcgatggag atcacttttg actcattttg
    12901 ttattcagtg atctccatca atgttattgg aacttcacag gtgtgttgat ctgtatcttt
    12961 tgccattccg gtaaaggata cctatgccaa cagttccaat ttctatgaga aaacttaaag
    13021 aaattcttag gcttaaatac ggtgttggac tcagccatcg acaaattggt cgtagtcttg
    13081 caatctcccc ttccgttgta tccagatatg ctaatcgggc ggctcaactt ggcataaagc
    13141 agtggccctt acctacagga tgggatgata caaaactaaa acatgcgttc cttcagaccc
    13201 aggttaagat gaagaagcac tctctgcctg actgggctac agtacaccgg gaactgcgta
    13261 ataaatgcgt gacgctgcag ctactctggg aagaatactg tgagcgtaat ccaggcggtt
    13321 tttacagcta taaccattac tgccggatgt accgtgaatg gctcaaaacc acttcaccat
    13381 caatgcgtca ggtacataaa gctggcgaaa aacttttcgt tgattactgt ggacctaccg
    13441 ttggcgttac cgaccctgag accggagaaa taagaactgc tcaggtcatc gtagctgttc
    13501 tcggggcatc aagttacaca tgggcagagg ccacctggtc tcagcagctt gaagactggg
    13561 tgatgagtca tgttcgctgc ttccagtggt tgggtggcgt tcctgaactt gttgttccgg
    13621 acaatctgaa aagcgccaca tccagggcat gtaagtatga tcctgacgtt aaccctacct
    13681 accagcagat gcttgagcat tataatgtcg cagttttgcc tgcgcggcca cgtaaaccga
    13741 aagataaagc caaagctgaa gttggcgttc aggttgttga acgctggatc atggcccgaa
    13801 tcaggcatga gatcttctac agccttgcat cgcttaatca gcgcattcgg gagttgctgg
    13861 aaagactgaa taacaaaata atgcagaagt tgggttattc acgtgcagaa ctcttcatcc
    13921 agcttgataa acccgcactg aagcctcttc ctgaagccag ttacagttac accctggtga
    13981 agaaagtcag agttcatgcc gattaccacg tggaaatcga caaacattac tactcggttc
    14041 catgttcgct gttaggccag caactggaag catggatctc cggagaactg gtaagactct
    14101 tcaatcaggg gcaggaggtt gctgtgcacc cgcgcaagcg tacttatggc tacagtaccc
    14161 gcaacgagca catgcctgaa gctcatcgac agcatgccac ctggacgcca gagcgtcttc
    14221 tggaatgggc ggggcacata ggcagtgaaa ctcatagtta tgtgcttcat atactgaact
    14281 ctcgtccaca tccggaacaa agctatcgct tctgccttgg actcctgaac cttcataaaa
    14341 aatacagtaa agccagactt aatgcagcat gtgcaagagc tctgaaaaca aaggtatggc
    14401 gtctgtcagg tattaaatcg atcctggaaa aaggtctgga taaacaacct gttcaggatc
    14461 caaaaccaga tctgttatcc acgatggaac acgaaaacgt acgcggcagt gagtattacc
    14521 actgatacgg gatccaatga tgaatcatct ttacgaacaa ctgaccgcac ttaaactcac
    14581 cggcttccgt gatgcgctta aaaagcaact tgctcagccg ggcacatacc aggagctggg
    14641 cttcgaagaa cgcctgtcat tactgacagc agaagaacta acctgccgtg aaaacaggaa
    14701 ggcagagcgt ctgatcaaac atgcacggtt cagacttaat gctgagttat caaagctgga
    14761 ttatcgtaac aatagagggc tggacagggc cctcatccgt tcactcagtc agggaaactg
    14821 gttaaccctg aaacaaaata ttttactgac cggggccacc ggcagcggta aaacgttcct
    14881 ggcatgtgca cttggtcata atgcctgccg acagggatac aaggtctact attatcgcct
    14941 taaagcgctg atggaacagt gctatcaggg gcatgctgat ggaagataca gcaaactttt
    15001 gaccaggctg aataatagcg atctgctgct tctggatgac tgggggctgg aacctctctc
    15061 atcagaacag cgtagcgacc tgctggaaat agtggatctg atgtaccaac gaggctcaat
    15121 catcgtagtg agccagttgc cggtggaaaa ctggtacaaa atgatcggag actccacaca
    15181 tgcggatgcc atcctagatc gactggttca tggcagtatc aagatcgaac ttaaaggaga
    15241 atcaatgcgg aaaatacaat ctccgttgac cgaaggagat cagtgaaggt aatttaaaaa
    15301 cggttctgtg aaagtgacac gaaccgatct ccatcgatgt tactcaccga
//
LOCUS       DAZSNO010000086        15333 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZSNO010000086 DAZSNO010000000
VERSION     DAZSNO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15333
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.086"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..557
                     /locus_tag="ACHWN2_002587"
     CDS             <1..557
                     /locus_tag="ACHWN2_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932072.1"
                     /translation="APQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGTLKN
                     YYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDAQIL
                     MFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLPDEK
                     VREIYALSEAKGDHLNLPQKAASA"
     gene            complement(830..1777)
                     /locus_tag="ACHWN2_002588"
                     /pseudo
     CDS             complement(830..1777)
                     /locus_tag="ACHWN2_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(1777..2370)
                     /locus_tag="ACHWN2_002589"
     CDS             complement(1777..2370)
                     /locus_tag="ACHWN2_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFR8932073.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            2517..2924
                     /locus_tag="ACHWN2_002590"
     CDS             2517..2924
                     /locus_tag="ACHWN2_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR8932074.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(3044..4036)
                     /gene="rpoS"
                     /locus_tag="ACHWN2_002591"
     CDS             complement(3044..4036)
                     /gene="rpoS"
                     /locus_tag="ACHWN2_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR8932075.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(4099..5193)
                     /gene="nlpD"
                     /locus_tag="ACHWN2_002592"
     CDS             complement(4099..5193)
                     /gene="nlpD"
                     /locus_tag="ACHWN2_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR8932076.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(5378..6004)
                     /gene="pcm"
                     /locus_tag="ACHWN2_002593"
     CDS             complement(5378..6004)
                     /gene="pcm"
                     /locus_tag="ACHWN2_002593"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR8932077.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(5998..6759)
                     /gene="surE"
                     /locus_tag="ACHWN2_002594"
     CDS             complement(5998..6759)
                     /gene="surE"
                     /locus_tag="ACHWN2_002594"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR8932078.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(6740..7789)
                     /gene="truD"
                     /locus_tag="ACHWN2_002595"
     CDS             complement(6740..7789)
                     /gene="truD"
                     /locus_tag="ACHWN2_002595"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR8932079.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(7786..8265)
                     /gene="ispF"
                     /locus_tag="ACHWN2_002596"
     CDS             complement(7786..8265)
                     /gene="ispF"
                     /locus_tag="ACHWN2_002596"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR8932080.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(8265..8975)
                     /gene="ispD"
                     /locus_tag="ACHWN2_002597"
     CDS             complement(8265..8975)
                     /gene="ispD"
                     /locus_tag="ACHWN2_002597"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR8932081.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(8994..9305)
                     /gene="ftsB"
                     /locus_tag="ACHWN2_002598"
     CDS             complement(8994..9305)
                     /gene="ftsB"
                     /locus_tag="ACHWN2_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR8932082.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(9499..9822)
                     /locus_tag="ACHWN2_002599"
     CDS             complement(9499..9822)
                     /locus_tag="ACHWN2_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR8932083.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(9872..10477)
                     /gene="cysC"
                     /locus_tag="ACHWN2_002600"
     CDS             complement(9872..10477)
                     /gene="cysC"
                     /locus_tag="ACHWN2_002600"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR8932084.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(10477..11904)
                     /gene="cysN"
                     /locus_tag="ACHWN2_002601"
     CDS             complement(10477..11904)
                     /gene="cysN"
                     /locus_tag="ACHWN2_002601"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR8932085.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(11906..12814)
                     /gene="cysD"
                     /locus_tag="ACHWN2_002602"
     CDS             complement(11906..12814)
                     /gene="cysD"
                     /locus_tag="ACHWN2_002602"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR8932086.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            13066..14103
                     /gene="iap"
                     /locus_tag="ACHWN2_002603"
     CDS             13066..14103
                     /gene="iap"
                     /locus_tag="ACHWN2_002603"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR8932087.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(14249..15333)
                     /locus_tag="ACHWN2_002604"
                     /pseudo
     CDS             complement(14249..15333)
                     /locus_tag="ACHWN2_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
BASE COUNT         3746 a         4104 c         3781 g         3702 t
ORIGIN      
        1 aagcacccca aggttctttt cctgccgatg gaaaagaacc gattatcgaa agaatcttta
       61 ggttagtaga acgataccct tcaagaaatg cggctgctcg ggcatggggg atcaatgaag
      121 gcactttaaa aaattattac aacaggcggg attacgcacc gataccacgc ccgtatcagt
      181 taaaaaagat agcagaaagc gaaaatgtta ccttggaatg gctcatgacg ggagagggtg
      241 atgttgaact aaaagattcc aaagaaccca aaaaagagaa tgcggataat gttgatgctc
      301 aaattctaat gttcttgtca tttcttaaac cacaagaaaa acaacaagta gctgatgttc
      361 ttgggagaaa aggagcggag caattactca ttcttctgga tgagcacatc caagagttgc
      421 acgctctagt tggagttcgt agagccatag cgttaagcct tggtaatttg ccagacgaaa
      481 aggttagaga gatttacgcg ttgtccgaag ctaagggcga tcacttaaat ctacctcaga
      541 aagcagccag cgcataaccc agtgcaagtg cctagctgtg ctaaaattac attggttttg
      601 gcgcttgcac ccatcagcgc tataataaac acctgaatcg tttttgaacg gtctttaaag
      661 gctattgtaa gaacctgtga gttatcttga atgttttatt gatatcgaac cgtttaacat
      721 ttttggactc gctttatctt gaaaaccttg agttcctaac ttgttgattc caatctattc
      781 ccgtcttttt taatctcttc ccggttatct tgaataaatt acctagtcat aggtagtcga
      841 tttcggtcca cggcataccg agatagagtg attcgaaaat cggtttgctg cgataagaga
      901 ctttatcgat gcgcaccacg gtcatgttgc gcccaccgga gtagtgcccg gtaaactcac
      961 cgaacggccc ttcgatttcg cgtttacggc tttcgataac cccttcgagg atcacttctg
     1021 aaccccacgg cacatcaaaa ccggtcagcg gagcggtggc gatcgggtac gggctttcac
     1081 gcagcgcgcc tgccatttca tactcagact gatcgtattt cagcggcgtg gcccccatca
     1141 gcgtgatgat cggatcgtta ccgagcgtga tcgcaatcgg cagatcttca ccgcgctctt
     1201 ctgctttatg cagatgcagg gcgatatcgt gcatcggcac cggttgcagg ccgagcttac
     1261 gcttgccctt cacttccatg cggtagatgc cgacgttctg cttgccgaag ttatccgggt
     1321 cgagcggatc gcgggaaacc acgcacgctt tgtcgagata gaaaccgcca tcgccatcat
     1381 tcaggcgaaa cagcggcaga atatcgaaca ggttgatctc ttcgccatcg acggtgttct
     1441 gcgcccaggc tggattggcg cggcgctccg gggcaatcgg gaagttatcc cagcggcgga
     1501 taaactcatc aatctgcttt ttgaccgggg tgtttggtgg caggccaagg gaaatcgcgt
     1561 ggttctgcca ggaaccgatg gtattcatcg ccacgcgggc atcggtaaag ccgcgaatat
     1621 tatcaaacca cagcgcgggc gcgccgtcgc cgatacgccc ggtggcgtta gctgctgctg
     1681 ccagatccgg ctcagcgttc acttcttcgc tgattttcag taactggccg tggtcatcaa
     1741 gcgcctgtaa aaagctgcgt aaatcatcaa atgccattat tcattctcct gagaaaaatt
     1801 ccgggcctgc ggcaatcctt gccagcgcct ggcgtgggga tgttcgaggc caaattgatc
     1861 cagcacgcgg gctaccacat ggtggacaat gtcatctacc gtttcgggat ggttataaaa
     1921 ggcaggcatc ggcggcacca tcgccacgcc catgcgtgaa agtgcgagca tattttcgag
     1981 atggatggtg ctaagcggca tttcacgcgg caccagcacc agtttgcggc cttctttgag
     2041 cacgacgtcc gccgcgcgcc ctaccaggcc atcggcgtaa ccagcgcgga taccggcgag
     2101 cgttttcata ctgcacggaa taacgatcat gccgtcggta cgaaaagaac cggatgagat
     2161 gatcgccgcc tgatccgccg ggttatggct gaagtctgcg agggcagcaa catcgcgagc
     2221 gctgtaaggc gtttccagtt caatggtggt tttcgcccac ttcgacatca ccagatgagt
     2281 ctcgacattc ggcatctcac gcagcgcttg cagtaatgcc acaccaagag gcgcaccggt
     2341 agcccctgtc atcccgacga tcagtttcat tgcttattct cttaattatt cgtatgcgaa
     2401 cgttattatt aacatactcc ttcatttcca cagcggcaag gattgaaaag taacaacatc
     2461 ttcttcgtca ctaaaaaacc gctatgatag cgacagagaa ttagcccgga gtttacatgg
     2521 cgttacgaaa taaagcgttc catcagttac gacagctttt tcagcagcac acggctcgct
     2581 ggcagcacga gttacctgac ctgaccaaac cacagtacgc ggtgatgcgc gccattgctg
     2641 ataaacctgg tattgaacag gtggcactga tagaagcagc agtcagcacc aaagcaacgc
     2701 tagcagaaat gttggcaaga atggaaaatc gtggcttagt cagacgtgaa catgacgtcg
     2761 ctgacaagcg gcgacgcttt gtctggctga ctgccgaagg agaaaagcta cttgctgcgg
     2821 cgatacctat tggtgacagc gtggatgagg aatttttggg gcgtttgagt ggagaagagc
     2881 aggagctgtt tatgcagttg gtgcgcaaga tgatgagcac atagaatgct gattgccggg
     2941 taggacgctg acgtgtctta tccaggcgac aaaaacacaa gcttcccaaa caaaagaaaa
     3001 aggccagcct cgcttgagac tggcctttct gacagatgct tacttacttg cggaacagcg
     3061 cttcgatatt cagcccctgc gtttgcagga tttcgcgcaa acggcgcagg ccttcaacct
     3121 gaatctggcg aacacgttca cgggtgaggc caatttcacg acctacatct tccagtgttg
     3181 ccgcttcata ccccagcaaa ccgaatcgac gtgccagtac ttcacgctgt ttggcgttca
     3241 gctcgaacag ccatttgacg atgctctgct tcatatcgtc atcttgcgtg gtatcttccg
     3301 gaccgttctc tttttcatcg gccaggatgt ccagcaacgc tttttcggaa tcaccaccca
     3361 gcggggtgtc taccgaggta atgcgctcgt taagacgaag catacggctg acgtcatcaa
     3421 ctggcttatc cagttgctct gcgatctctt ccgcacttgg ttcatggtcc agcttatggg
     3481 acaactcacg tgcggttcgc aggtaaacgt tcagctcctt tacgatgtga atcggcaaac
     3541 gaatagtacg ggtttggttc ataatcgccc gttcaatcgt ctggcgaatc caccaggttg
     3601 cgtatgttga gaagcggaaa ccacgttccg ggtcaaactt ctctaccgcg cggatcagcc
     3661 ccaagttgcc ctcttcgata aggtccagca acgccagacc acgattgcca taacggcggg
     3721 caatttttac caccagacgc aagttactct cgatcatccg gcggcgagag gcgacatctc
     3781 cacgcagtgc gcgacgcgca aaataaactt cttcttcggc cgttaacagt ggtgaataac
     3841 caatctcacc aaggtaaagc tgagtcgcgt ccaacacacg ctgtgtggct ccctgcgata
     3901 acagttcctc ttcggccaaa tcgttatcac tgggttcctc ttctactaag gccttttcgt
     3961 caaaaacctc aactccgttc tcatcaaatt ccgcatcttc atttaaatca tgaactttca
     4021 gcgtattctg actcataagg tggctcctac ccgtgatccc ttgacggaac attcaagcaa
     4081 aagcctggtt ccgccgattt atcgctgcgg caaataacgc agcgggttta cggatttccc
     4141 cttgtaacga atttcaaaat gcaagcgtgt tgaactggtt ccggtgctac ccatggttgc
     4201 tattttttgc cccgccttca cttcttgttg ttcccggacc agcattgtgt cgttatgggc
     4261 gtaggcactc aggtaatcat cattatgttt gatgataatc agattaccgt agccgcgcag
     4321 cgcgttacca gcataaacaa cgcggccatc tgcggtcgcg ataattgcct gtcctttgct
     4381 gcctgcgata tcaatcccct tgttgccccc ctcagaagcg ccaaaggttt cgatcacttt
     4441 gccctcagtc ggccagcgcc aggtggagat aggcgtactg gttgatgtac tgctgacagt
     4501 cggctcggtt gtgcttgctg ttggtaccgt tacaggcgct gtgaccgtgg tcgcagttgg
     4561 cttgttgttc ggcaacattt tgttagcact ctgttcaccc gaagactcag aatacgtaat
     4621 tgtcggttgc gacgcaacag caacggtgga attttgtgca ggcttgatca caactccttg
     4681 ctctgctgcg tcggcctggg taatggcatt tccgccagtg attggcgtac cggaagcatt
     4741 acccacctgc aaggtctgac caacgttcag cgcgtatggt gcctgaatat tgttgcgctg
     4801 agcaaggtca cggaaatcgt tgccagtaat ccaggcgata tagaaaagtg tgtcgccttt
     4861 tttcacggta taggtactgc cgctataact gcctttcgga atgttcccat actgacggtt
     4921 atagacgatg cgtccgtttt ccatctgtac cggctgctga gctactggct gcactggctg
     4981 gatttgcggt tgttgagtag cctgaatttg tggctgctgt accggctgaa tttgcggttg
     5041 ctgcgctgta gacgtcgtcc ccattttcgg cggcggcgta atcaacatac cagaattagt
     5101 atttgcaggc gcattgccat taacggagct gaccggtgcc ggtggatttg aagtgtcaga
     5161 acagcctgcc agccatagcg aaaccagtga caaagccgca atgcggcgaa cggtgaattt
     5221 tgggcttccc gcgctcattt atcccccagg aaaaattggt taataaccag tgacataatt
     5281 accgtgcaag gcaccctact gaacactgga aaagatgttc acgatacgct gacctacggc
     5341 aaaataacca ggaaaaatcc aggtatttcc tcacgtttta agccagctca cccttcacta
     5401 aagggacaaa gcgcacggcc tccacggtat cgataataaa ttcgcctccc cgacgacgca
     5461 cccgtttcaa atactggtgc tcctccccta cgggtaagac gagaatcccg ccttcgtcca
     5521 gctgcgtcat tagcgcagtt ggaatttccg gcggtgccgc cgtaacaatg atagcgtcaa
     5581 acggcgcacg tgcctgccaa ccttgccatc catcaccatg acgggttgaa acattatgta
     5641 aatcaagatt tttcaggcgg cgacgtgcct gccactgcaa gcctttaatc cgttcaaccg
     5701 agcaaacatg ctggacaaga tgcgccagga ttgccgtttg atatcccgaa ccagtgccaa
     5761 tttccagcac ccgcgactgc ggcgtcagct cgagtaattc ggtcattcgc gccaccatat
     5821 acggctgcga aattgtctgc ccctgaccta tcggcaaggc gatattgtcc caggcttttt
     5881 gttcaaacgc ttcatcaacg aatttttcac gcggcacggc ggcaagtgca ttcagcacct
     5941 gctcatcctg aataccttgc gcacgtaatt gatccagaag tgcttgtacg cgtctgctta
     6001 ccattgcgtg ccaactccca cgctgtttaa ccagtctgaa accacatctt gcgcgctatg
     6061 cgcagttaaa tccacatgca gcggcgtgat ggagacatag ccctcatcta ccgcagcaaa
     6121 atcggtcccc ggaccggcat cacatttacc gcccggcggg ccaatccagt acagcgtatt
     6181 gccgcgcgga tcttgctgcg ggatcacctg atctgccgga tgtcgtgtac cgcagcgcgt
     6241 cacgcgaata cctttgattt gatccaaggg taaatcctga acgttaatat taagaatacg
     6301 tccggtgcgc agcggctctt tacacagtgc gcgcaaaatt gaacaggtta ccgccgcggc
     6361 agtgtcgtaa tgtttatgcc cgtcaagcga gacggccagc gccggaaaac ctaaatgacg
     6421 gccttccatc gcggcggcta ccgtaccgga ataaataaca tcatccccca gattcggccc
     6481 ggcgttaatt ccggacacaa caatgtccgg gcgcggacgc atcagagcat tcacgccaag
     6541 atagacacaa tcggtcgggg ttcccatttg cacagcaata tcaccatttt caaaggtaaa
     6601 cgtgcgcagg gaggattcca gtgtcagaga atttgaagcg ccgctgcggt tacgatcggg
     6661 ggcgaccacc tgaacgtcag caaactcacg caaggctttc gccagcgttt gtataccggg
     6721 tgcatgtacc ccgtcataat tactcagcaa tatgcgcata atcacctgtt gtgttgataa
     6781 gttccctgac aacgctggtt gcaaaactac ccgccggaag ccagaaacgg atctctacgg
     6841 tgacgtcatc ccaccagttc cagcttaatt gttgcggata cagcagcatc gctctgcgcg
     6901 cggcttcaac tttttcgcgc accagtaaag cttgtaattc agtttctgcg gcgacagctg
     6961 cttgttcgaa tgccagcgct tcacgctgag ttccccattc gccactgccc ggcaatgcgg
     7021 cggttatcat cagctcttta tcgttgacgc gacgctgtaa ttccgccagt tcttcggtgg
     7081 ttgctacaaa ccagctacca cgtccggcta attgtagcgc atcgccgtca acaacttgat
     7141 taacgtctgc ttttttgagg cgctcagcaa caatctgatt aaacaacgca ctgcgggctg
     7201 ccgacaacca aaaactccgt ttattgcgat cgcgcaccgg agtattggtt tgcgcccagc
     7261 gcagcgcgcc ctgcaagttg ctaccgccaa tcccaaaacg ttgggcaccg aagtagttcg
     7321 gtacaccttt tacgcaaata tcgatcagac gttgttcaac gtcatcgcga ttgctcactt
     7381 cgcgcagaac cagggtaaag gcgttacctt tcagcgcccc taaacgcagc ttgcgcttgt
     7441 gccgcgcata ctccagcacc tggcagcctt ccagttgaaa ggcgctcaga tcgggcattt
     7501 ccttgcccgg cacgcgagca cataaccact gttccgttac agcatgtttg tctttttgcc
     7561 cagcgaagct gacttcacgg gaatgaattt tcaggaattt cgccagtgca tccgccacaa
     7621 aacgggtatt gcagccgttt ttgaggattc taaccagaat atgctcacct tcaccatcag
     7681 gctcaaagcc caaatcttcc accaccacaa agtcttccgg attggctttc agcagcccgg
     7741 tgccttgcgg tttaccgtgg aggtaagtga gattatcaaa ctcaatcatt ttgttgcctt
     7801 aatgagtagc gccaccgctt cacaggcaat cccttcccca cgtccggtaa atccaagttt
     7861 ttccgtagta gtggctttca cgttaacatc atccatatgg cagccgagat cttcggcaat
     7921 aaacacgcgc atttgtggaa tgtgcggcaa catcttcggt gcctgagcga tgatagtgac
     7981 atcgacgttg ccaagggtat aacccttcgc ctgaatacga cgccaggctt cgcgtagcag
     8041 ctcgcggctg tcggcacctt taaatgccgg atcggtatcc gggaacagtt tgccgatatc
     8101 ccccagcgcc gccgcgccaa gcaatgcatc ggtcaacgca tgaagcgcca cgtcgccatc
     8161 agaatgcgcc agcaatcctt tttcgtaagg aatgcgtacg ccaccaatga taattgggcc
     8221 ttcaccgcca aaggcgtgta cgtcaaaacc gtgtccaatt cgcattatgt attctcctga
     8281 tggatggttc gggtgaggta aaactcggcc agtgccaaat cttccgggcg cgtgacttta
     8341 atgttatccg cgcggccttc gaccaactgc ggatgaaatc cgcaatattc cagcgccgag
     8401 gcttcgtcgg taatggtagc gccttcattt agagcgcgcg tcagacagtc atgtaacagc
     8461 tcacgaggga aaaattgcgg cgtcagcgcg tgccataagc cgttgcgatc aacggtatga
     8521 gcaatggcat ttttgcccgg ttcggcacgt ttcatagtat cgcgcactgg tgcggcgagg
     8581 atccctcccg tgcggctggt ttcgctcaac gccaacaatc gcgcaaggtc atcctgatgc
     8641 aaacaaggac gagcggcgtc atgcaccaat acccactgca catcgccagc ggctttcaga
     8701 cctgccagca cggaatcggc acgctcatca ccgccatcta caacggtgat ttgcggatga
     8761 ttcgccagag gaagttgtgc aaaacggcta tcgccaggac ttatggcaat gacgacacgt
     8821 gtcacccggg gatgcgccag cagcgcatgc accgagtgtt caagaatggt ttgattaccg
     8881 attgagagat attgcttagg acattccgtt tgcattcgac ggccaaatcc ggccgccgga
     8941 accacggcgc aaacatccaa atgagtggtt gccatgttaa ttcccgggct gatttatcga
     9001 ttgttttgcc ccgcagactg tgcgcgcttc gacgcgtcag gcaccagacg ataaaaagtt
     9061 tcgcccggcc tggtcatgct gagttcatta cgcgcacgct cttcgagcgc ctcctggccg
     9121 ccattgagat cgtcaatttc ggcaaaaagt tgatcgtttc gcgctttaag tttcgcgttt
     9181 gtagcttgca gtgccgccac atcatcattg acgcgggtat agtcatgtat accgttctta
     9241 ccgaaccaca gcgaatactg tagccagacc agaatagcca gcaacagcag cgttagttta
     9301 cccatcctgc cccctgaaaa actgcatcat catcccatgc atccgaagac gactctacat
     9361 cctctgttgg ggataccgcg acaacgcggg caaatgtacc acatttgccc attgttacgt
     9421 atacccaggg cgtgcagaac ataatctcat tattagttac ggtttgaatt atgaacagag
     9481 gagacaagaa agtacaaatt agcccagtag ccacataaac agtgcgccaa acataatgcc
     9541 tactgtcacc agggtgaaaa caatactgta gcgtagcttt ccgtccatca atgaatgcag
     9601 cgcaatcccc accactaccg cgacaggcat cagtgccaga aagaaaggcc aggtgtagag
     9661 aaagaagaac agcgtgttag agccataaat caacatcggc atcgccagcg caaataacca
     9721 ggagataaaa ccgaccacgg caccaggcag tgaccatgta gtttcttcat cctcagtaag
     9781 gctgtcgtta tttgttagtg taatgttatg gctattacgc atatttgatc ctgttacttt
     9841 gacgaaccgg gcatggaaac ccggtggtgt ctcaggatct gataatatcg ttctgtctca
     9901 acagatctaa taattgctgt accaaatttg ttactaattg ttcaccattg agatgaattt
     9961 ctgccgattc aggcgcttcg taaacggaat ctattcccgt aaagttgcgc agttcaccgg
    10021 cacgcgcttt cttatataag ccttttggat cgcgggcttc gcaaatcgcc agcggcgtat
    10081 cgacaaacac ttcaataaag cgcccttctc ctacgcgttc gcgaaccatc tggcgttcgg
    10141 cgcggtgtgg cgagataaat gcggtcagca ccaccagtcc ggcttcaacc atcaaattcg
    10201 ccacttcacc gacgcgacgg atattctctt tacgatcggc atcgctaaaa ccgagatcgc
    10261 tgcataatcc gtggcgaaca ttgtcgccat ccagcagata cgtactgacg ctgagtttat
    10321 gtaacgcctc ctccagcgcc ccggcgaccg ttgatttacc ggacccggag aggccggtaa
    10381 accacagcac tacaccacga tgaccgtggt gtagttcgcg ttgttgcaca gtgaccggat
    10441 gactatgcca gacgacgttt tcgtcatgca gcgccattat ttatccccca gcaaatcgcg
    10501 cgcgccccag tgcggaaagt ggcgacgaac cagggcattc aattccagtt cgaatgcact
    10561 gaattcagat gacgcagctg ttgcctggct aactggctcg tgcaccatac cggcacccac
    10621 ggtcacattg ctcaggcgat cgataaaaat cagcccaccg gtaaccggat tttgctgata
    10681 gcgatctaac accagtggct cgtcaaaagt gagatccacc aggccgatgc cgttcagcgg
    10741 caggttttca acttcgcgtt gggtaaggtt attaatatca acctgataac gaatgccatc
    10801 aacacgagca cgcgtcttct taccggcaat tttgatgtca taactctggc ccggggaaag
    10861 cggctgttcc gccatccata ccacatccac cgacgcactc tgcaccgccg gtaacgcttc
    10921 gtctgccgcc agcagcagat caccacggct gatatcgatc tcatccgtca gcaccagggt
    10981 gatcgcttct ccggcaaaga cttcttcgcg atcaccatca aaagtcacga tccgcgcgac
    11041 gtttgattcc acaccagagg gcagcacttt tacacgttgc ccgacttcca cgcgaccgga
    11101 tgccagcatt ccggcgtagc cacgaaaatc gagatttggg cggttaacgt actgcaccgg
    11161 gaagcgcatt ggctgggcat ccaccactcg ctggatctcc acggtttcca gcacttcgag
    11221 cagtgtcaga ccgctgtacc acggcatact ttcactttgc gaagccacgt tgtcaccttc
    11281 cagtgcggag agcggcacaa agcggatatc cagattaccc ggcagctgcc cggcaaaggt
    11341 cagataatct tcacgaatac gggtgaacgt ctcttcactg taatccacca gatccatttt
    11401 gttgatcgcc acgaccagat gtttgatccc caacagtgtg gagataaaac tgtgacgacg
    11461 ggtttgatcg agcacgcctt tacgggcatc aatcagtaag atcgccagtt cacatgtcga
    11521 tgcgccagtc gccatattgc gagtgtactg ctcgtgccct ggggtgtcgg cgataataaa
    11581 tttacgcttc tcggtagaga aatagcggta ggccacatca atagtgatgc cctgttcgcg
    11641 ctcagcttgc aggccgtcca ccagcagagc cagatccagc ttttcgccct gggtgccgtg
    11701 acgcttactg tcgttatgca gcgatgagag ctgatcttcg tagatttggc gggtatcgtg
    11761 cagcagacga ccaatcaggg tacttttgcc gtcatcgacg ctaccacagg tcagaaaacg
    11821 cagcaggctt ttatgttgtt gcgcaatcat ccaggcttcg acgccgcctt cattggcgat
    11881 ttgttgtgca agtacggtgt tcatcttaaa aatacccctg acgttttttc agctccatgg
    11941 atcccgcctg gtcgcggtca atcacgcgac cctgacgttc actggtggtg gaaaccagca
    12001 tctcttcgat gatttccggc agtgtttgtg catttgactc caccgcaccg gtcagcggcc
    12061 agcagcccag cgtacggaaa cgcaccatcc gttttttgat cacttcgccc ggttgcaggt
    12121 cgatacggtt gtcatcaatc atcatcaaca taccgtcgcg ttccagaacc ggacgttccg
    12181 cagcgagata cagcggcaca atgtcgatat tttccagcca gatgtattgc cagatatcct
    12241 gctcggtcca gttagagagc gggaagacgc ggatgctttc gcctttgtta atttgcccgt
    12301 tgtagttgtg ccacagctcc gggcgctgat ttttcggatc ccagcgatgg aagcggtcac
    12361 ggaaagagta gattcgctct ttagcgcggg atttctcttc gtcacggcgc gcaccaccga
    12421 aggcggcatc aaaaccgtat ttgttcagcg cctgtttcag gccttcagtt ttcataatat
    12481 cggtatgttt cgcgctgccg tgcacgaatg gattaatccc catcgccacg ccttccgggt
    12541 ttttatgcac cagcagctcg cagccgtagg ctttcgccgt acgatcgcgg aactcataca
    12601 tctcgcggaa tttccagccg gtatcgacat gcagcaacgg gaaaggcagc gtacctggat
    12661 aaaacgcctt gcgcgccaga tgcagcatga cgctggaatc tttaccgata gagtagagca
    12721 tcaccggatt tgagaattct gccgccacct cgcgaataat gtggatgctt tccgcctcca
    12781 gttgccgcag gtgagtaagt cgtttttgag ccataaccgt tcctttgcaa taccgctatt
    12841 ttcttgccat cagatgtttc gactataggg agcgtaagag aacgaatgaa attaccaatt
    12901 agaatgagta gttccttaac ggaataacga tttggcaaag ctaatatcaa aaagtgctta
    12961 aggcaccgga tttcgggcgt ttaggaagat ttgaaattgt tttagcgcag cggcagtttc
    13021 atactatggc ggtaaaaaaa tttgcatggt atttaaggac tcactatgtt ttccgcattg
    13081 cgccaccgta ccgctgccct ggcgctcggc gtatgcttta ttctccccgt acacgcctcg
    13141 tcacctaaac ctggcgattt tgctaatacc caggcacgac atattgctac tttctttccg
    13201 ggacgcatga ccggaactcc agcagaaatg ttatctgccg attatattcg ccaacagttt
    13261 cagcaaatgg gttatcgcag tgatattcgg acatttaata gtcggtatat ttataccgcc
    13321 cgcgataatc gcaagaactg gcataacgtg acaggaagta cggtgattgc cgctcatgaa
    13381 ggcaaagcgc cgcagcagat catcattatg gcgcatctgg atacttacgc cccgctgagc
    13441 gatgctgacg cagatgccaa tctcggcgga ctgacgttac aaggaataga tgataacgcc
    13501 gcaggtttag gtgtcatgct ggaactggca gaacgcctga aaaatacgcc taccgagtat
    13561 ggtattcgat ttgtggcgac cagcggcgaa gaggaaggaa aattaggcgc tgagaattta
    13621 ctcaagcgga tgagtgacac cgaaaagaaa aatacgctgc tggtgattaa tctcgataac
    13681 ttaattgttg gcgataaatt gtatttcaac agcggtgtaa aaacccctga agcagtaagg
    13741 aaattaacgc gcgacagggc gctggcaatt gcacgcagtc acggaatagc cgcaacgacc
    13801 aatccgggtt tgaataaaaa ttatccgaaa ggcactgggg gttgtaatga cgcagaaata
    13861 ttcgacaaag cgggcattgc tgtactttcg gtggaagcga ctaactggaa tcttggaaat
    13921 aaggatggtt atcagcaacg cgcaaaaaca cctgccttcc cggcgggaaa tagctggcat
    13981 gacgtaagac tggataatca acaacatatt gataaggcac ttcctggaag aatagaacgt
    14041 cgctgccgtg acgttatgcg gataatgcta ccgttggtga aggagctggc gaaggcgtct
    14101 tgatgggttt gaaaatggga gctgggagtt ctaccgcagg ggcggggaat tctaagtgat
    14161 acccatcatc gcatccagtg cgtccggttt acttccggga aaaccgcacc gtattgcgcc
    14221 ggtttatctc cgctggagcg gggaacacct ctggcgaata gaaagcaagc caggttttga
    14281 ccagtcgatc gtacatggca ttggccgttg ccattgttgg caggccaaaa aacagcccct
    14341 gagcgttacc cgcagccatc aacctgtgcg ccaggataag cgccgcttct gttttacctg
    14401 cgccggtcac atcttccaga ataaacaact gtggtcctgg ctgattgata tccagatcca
    14461 gcaccttttg ctgtaacggt gtcgtattct caataaaagg aaacagcgta ttaattccgg
    14521 tgaaaggcgc ggtttctgct tgtggcggaa agacggttaa tgcgttttga gcctgaacta
    14581 aagccttttg ccagtaatct tcaatatcca ttgcctgtgc gacgcgtgga aaaaatcgcg
    14641 ttgacgaccc tgtccagtct gcaagtacga cggttgcaga gatataccag gaaagttgtt
    14701 ttataagttc aacgccctcg tcatcatccc agaatgtggg gatctctatt cgcggaaaca
    14761 gtggcttgat ttcaaggaga aaatctcgtg ctgcagcttt gtcttcaggc agaaaattat
    14821 ccaattcatc catacgatca gggggccgac catgatgccc tgtcgtcacg ggcatccaca
    14881 tttctatcac tcgcctaagt ttgcgcggag agagcgaaga tgaaggaagt aactcctgac
    14941 attcacttaa ataatgattc cacaaccagt agcccagcgt tgaatgagag attttttcgt
    15001 aattttttct ggcaccttcc tgaatcttga attcaggggg caggtaaagt tgttgaaaag
    15061 agcgggcaaa ttttccaata tcgtgccagc acagcaacca ggcgaaaaat cgagctgcct
    15121 gttccttgtc agaaatccct aattgacgaa aataatcagc cagcccgaag caatttcttt
    15181 taaccattaa atagcccatt gcggccacat ccagcaaatg ccagcaaaga aggtgatagt
    15241 cgtcgatacc ctctttctcg ccacgtcggt tttttcccca gaaatcaaag aaagtcacaa
    15301 tatttttatc cttcagtaaa cttaaaggat att
//
LOCUS       DAZSNO010000087        15175 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZSNO010000087 DAZSNO010000000
VERSION     DAZSNO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15175
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.087"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(248..1072)
                     /gene="iclR"
                     /locus_tag="ACHWN2_002605"
     CDS             complement(248..1072)
                     /gene="iclR"
                     /locus_tag="ACHWN2_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR8932088.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1272..4955
                     /gene="metH"
                     /locus_tag="ACHWN2_002606"
     CDS             1272..4955
                     /gene="metH"
                     /locus_tag="ACHWN2_002606"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR8932089.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5175..6806
                     /locus_tag="ACHWN2_002607"
     CDS             5175..6806
                     /locus_tag="ACHWN2_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR8932090.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6897..7586)
                     /gene="pepE"
                     /locus_tag="ACHWN2_002608"
     CDS             complement(6897..7586)
                     /gene="pepE"
                     /locus_tag="ACHWN2_002608"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR8932091.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(7968..9197)
                     /gene="sorE"
                     /locus_tag="ACHWN2_002609"
     CDS             complement(7968..9197)
                     /gene="sorE"
                     /locus_tag="ACHWN2_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFR8932092.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            complement(9249..10037)
                     /locus_tag="ACHWN2_002610"
     CDS             complement(9249..10037)
                     /locus_tag="ACHWN2_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HFR8932093.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            complement(10048..10845)
                     /locus_tag="ACHWN2_002611"
     CDS             complement(10048..10845)
                     /locus_tag="ACHWN2_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR8932094.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            complement(10911..11405)
                     /locus_tag="ACHWN2_002612"
     CDS             complement(10911..11405)
                     /locus_tag="ACHWN2_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFR8932095.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(11405..11812)
                     /locus_tag="ACHWN2_002613"
     CDS             complement(11405..11812)
                     /locus_tag="ACHWN2_002613"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR8932096.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            complement(11822..12628)
                     /locus_tag="ACHWN2_002614"
     CDS             complement(11822..12628)
                     /locus_tag="ACHWN2_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8932097.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(12698..13645)
                     /locus_tag="ACHWN2_002615"
     CDS             complement(12698..13645)
                     /locus_tag="ACHWN2_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFR8932098.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            13993..14865
                     /gene="rluF"
                     /locus_tag="ACHWN2_002616"
     CDS             13993..14865
                     /gene="rluF"
                     /locus_tag="ACHWN2_002616"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR8932099.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(14998..>15175)
                     /gene="yjbD"
                     /locus_tag="ACHWN2_002617"
     CDS             complement(14998..>15175)
                     /gene="yjbD"
                     /locus_tag="ACHWN2_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR8932100.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
BASE COUNT         3619 a         3953 c         3995 g         3608 t
ORIGIN      
        1 tacaactaaa aaaatatcat aaatcgctca atctcataat gcagccgtaa aagtttcggt
       61 ggaatgagat cttgcgattt tgttaataat taagtggttt gatgtttaaa aataatgctg
      121 agttaataaa agtgtgtgaa gtgtatgaca ttaagttatt ttggcgtcaa tgcgattaac
      181 agacaccctt attctattgc cactcaggta tgatgggcag aatattgcct ctgcccgcca
      241 gaaaaagtca gcgcattcca ccgtacgcca gcgtcacttc cttcgccgct ttaatcacca
      301 tcgcgccaaa ctcggtcacg cgatcatcgg taatacgtga aatcggtccg gaaatagaaa
      361 ttgcggcaaa cggttcgcgg tgctcatcga aaatacacgc tgcaaggcaa cgtagcccca
      421 gcgcatgttc ctcatcgtca aatgaataac cccgtttgcg cgtttgggcg agatcttctt
      481 ttaaatgcac aggagacacc agagttgcgt gggtataggc atgtaaccct ttgcggtgca
      541 gcagcttcgt cacctgttct tcgctcagtt gggctaaaaa ggctttaccc gcaccggaag
      601 cgtgcatcgg caatttaccg ccgataggcg cggacattcg catcaggtgg gtacactgta
      661 cctggtcgat aataatcgct tcgtgatcgc tttgatcaag caccgccata ttgaccgttt
      721 cgccagactc ttccattaga ttgcgcagga tagggtgaac aattgctaac aaattacggc
      781 tctggagaaa gctgctgccg accataaagg catgtgcgcc gattgcccag tgtcccagtt
      841 cgccgacctg acgcacgaaa ccctgctgtt gcatcgtggt tagcaggcgg tgggtcgtgg
      901 aattgggtaa cccggcttgt tgcgccagct ctgtgagtgc cacgctgcca ttggattcgg
      961 caatccactc cagtaatttc aggccacgcg ttaaagactg aacctgtcca gtcgctggtg
     1021 cggtggcaac ggcgggtttt ctgccgcgtt tcgcgggaat gggtgcgacc atgacagcct
     1081 cctttttctg tatcgtggaa attattttca tttttattgt tagctaatgc aatagttgct
     1141 gaactgatcc gatgagttaa tgttgaacaa atctcatgtt gcgtggtggt cgcttttacc
     1201 acagatgcgt ttattccagt atggtttgtt gaatttttat taaatctggg ttaagcgtgt
     1261 cgggagcaag tgtgagcagc aaagtggaac aactgcgtgc gcagttaaat gaacgtattc
     1321 tggtgttgga cggcggtatg ggcaccatga tccagagcta tcgactgaac gaagccgatt
     1381 ttcgtggtga acgctttgcc gactggccat gcgacctcaa aggcaacaac gacctgctgg
     1441 tactcagtaa accggaagtg atcgccgcta tccacaacgc ctactttgaa gcgggcgcgg
     1501 atatcatcga aaccaacacc ttcaactcca cgaccattgc gatggcggat taccagatgg
     1561 aatccctgtc ggcggaaatc aactttgcgg cggcgaaact ggcgcgcgcc tgtgctgacg
     1621 agtggaccgc gcgcacgccg gagaaaccgc gctacgttgc cggtgttctt ggcccaacca
     1681 accgcacggc gtctatttct ccggacgtca acgatccggc attccgcaac atcacttttg
     1741 accagctggc ggcggcctat cgcgaatcca ccaaagcgct ggtggaaggt ggtgcggatc
     1801 tgatcctgat tgaaaccgtt ttcgacaccc ttaacgccaa agcggcggtc tttgcggtga
     1861 aaacggagtt tgaagcgctg ggcgttgagc tgccgattat gatctccggc accatcaccg
     1921 acgcctccgg gcgcacgctc tccgggcaga ccaccgaagc attttacaac tcattgcgcc
     1981 acgccgaagc gctgaccttt ggcctgaact gtgcgctggg gcccgatgaa ctgcgccagt
     2041 acgtgcagga actatcacgg attgcggaat gctacgtcac cgcccacccg aacgccgggc
     2101 tgcctaacgc ctttggtgaa tacgatctcg acgccgacac gatggcaaaa cagatacgtg
     2161 aatgggcgca agcgggcttt ctcaatatcg tcggtggttg ctgcgggacc acgccgcaac
     2221 atattgcagc gatgagtcgt gcagtagaag gattagcgcc gcgcaaactg ccggaaattc
     2281 ccgtagcctg ccgtttgtct ggcctggagc cgctgaacat tggcgacgat agcctgtttg
     2341 tgaacgtggg tgaacgcacc aacgtcaccg gctctgctaa gttcaagcgc ctgatcaaag
     2401 aagagaaata cagcgaggcg ctggatgtgg cgcgtcagca ggtggaaaac ggcgcgcaga
     2461 ttatcgatat caacatggat gaagggatgc tcgatgccga agcggcgatg gtgcgttttc
     2521 tcaatctgat tgccggtgaa ccggatatcg ctcgcgtgcc gattatgatc gactcctcaa
     2581 aatgggatgt cattgaaaaa ggtctgaagt gtatccaggg caaaggcatt gttaactcta
     2641 tctcgatgaa agagggcgtc gacgccttta tccatcacgc gaaattgttg cgtcgctacg
     2701 gtgcggcagt ggtggtgatg gcctttgacg aacagggaca ggccgatact cgcgcacgga
     2761 aaatcgagat ttgccgtcgg gcgtacaaaa tcctcaccga agaggttggc ttcccgccag
     2821 aagatatcat cttcgacccg aacatcttcg cggtcgccac cggtattgaa gagcacaaca
     2881 actacgcgca ggactttatc ggcgcgtgtg aagacatcaa acgcgaactg ccgcacgcgc
     2941 tgatttccgg cggcgtatct aacgtttctt tctcattccg tggcaacgat ccggtgcgcg
     3001 aagccattca cgcagtgttc ctctactacg ctattcgcaa tggcatggat atggggatcg
     3061 tcaacgccgg gcaactggcg atttacgacg acctacccgc tgaactgcgc gacgcggtgg
     3121 aagatgtgat tcttaatcgt cgtgacgatg gcactgagcg tttactggag cttgccgaga
     3181 aatatcgcgg cagtaaagcg gacgacactg ctaacgccca gcaagcggag tggcgctcgt
     3241 gggacgtgaa taaacgtctg gaatactcgc tggtcaaagg cataaccgag tttatcgagc
     3301 aggatactga agaagcccgc cagcaggcta cgcgcccgat tgaagtgatt gaaggcccgt
     3361 tgatggacgg catgaacgtg gtcggcgatc tgtttggcga aggcaaaatg ttcctgccac
     3421 aggtggtcaa atcggcgcgc gtcatgaaac aggcagtggc gtatctcgaa ccgtttattg
     3481 aagccagcaa agagcagggt aaaaccaacg gcaagatggt gatcgccacc gtgaaaggcg
     3541 atgtccacga catcggtaaa aatatcgttg gtgtggtgct gcaatgtaac aactacgaaa
     3601 ttgtcgatct cggcgttatg gtgcctgcgg aaaaaattct ccgtatcgcc aaagaagtga
     3661 atgctgattt gattggcctt tcggggctta tcacgccgtc gctggacgag atggttaacg
     3721 tggcgaaaga gatggagcgt cagggcttcg ccattccgtt actgattggc ggcgcgacca
     3781 cctcaaaagc gcacacggcg gtgaaaatcg agcagaacta cagcggccct acggtgtatg
     3841 tgcagaatgc ctcgcgcacc gttggtgtgg tagcggcgct gctttccgat actcagcgtg
     3901 atgattttgt cgctcgtacc tgcaaggagt acgaaaccgt gcgtattcag cacgggcgca
     3961 agaaaccacg cacaccaccg gtcacgctgg aagcggcgcg cgataacgat ttcgcatttg
     4021 actggcaggc ttacacgccg ccggtggcgc accgtctcgg cgtgcaggaa gtggaagcca
     4081 gcatcgaaac gctgcgtaat tacatcgact ggacaccgtt ctttatgacc tggtcgctgg
     4141 ccgggaagta tccgcgcatt ctggaagatg aagtggtggg cgttgaggcg cagcggctgt
     4201 ttaaagacgc caacgacatg ctggataaat taagcgccga gaaaacgctg aatccgcgtg
     4261 gcgtggtggg cctgttcccg gcaaaccgtg tgggcgatga cattgaaatc taccgtgacg
     4321 aaacgcgcac ccatgtgatc aacgtcagcc accatctgcg tcaacagacc gaaaaaacgg
     4381 gattcgctaa ctactgtctc gctgacttcg ttgcgccgaa gctttctggt aaagcagatt
     4441 acatcggcgc atttgccgtg actggcgggc tggaagagga cgcactggct gatgcctttg
     4501 aagcgcagca cgatgattac aacaaaatca tggtgaaagc gcttgccgac cgtttagccg
     4561 aagcctttgc ggagtatctc catgagcgtg tgcgtaaagt ctactggggc tatgcgccga
     4621 acgagaacct cagcaacgaa gaactgatcc gcgaaaacta ccagggcatc cgtccggcac
     4681 cgggctatcc ggcctgcccg gaacatacgg aaaaagccac catctgggag ctgctggaag
     4741 tggaaaaaca tactggcatg aaactcacag aatctttcgc gatgtggcct ggtgcatcgg
     4801 tttcgggttg gtatttcagt catccggaca gcaagtacta cgcggtggcg caaattcagc
     4861 gcgatcaggt tgaagaccat gcccgccgta aaggtatgag cgtctccgat gttgagcgct
     4921 ggctggcacc gaatctgggg tatgacgcgg actgattcac aaatctgtca cttttcctta
     4981 caacaaacag ggcgctcaat gagtgccctg tctctttatt aatatgaaac acttatactg
     5041 gaaacaggct ggaataatct tagccggaaa aacgaatgtc aaaaggcaca gtaaagatgc
     5101 cagtcagcgt gtttccggtc ctttggtcat tcaattgctt caggaacata taacgataag
     5161 gagaatctga actcgtgtta acgctgcttc acctgctttc tgccgtcgcc ctgctggtct
     5221 gggggactca tattgttcga accggcgtaa tgcgcgtctt cggcgcgcgt ttgcgtactg
     5281 tccttagccg gagcgtcgaa aaaaagccgc tcgccttttg cgcggggatc ggcgttaccg
     5341 cactggtaca gagcagtaat gccaccacca tgctggtgac ctcgttcgtc gctcaggatc
     5401 tggtagccct cgcaccggct ctggtcattg tgctgggggc agatgtcggg acggcgctaa
     5461 tggcgcgtat tctcaccttc gacttatcct ggctgtcacc gttacttatt tttatcggcg
     5521 tgattttttt cctcggacgc aaacagtcac gcgccgggca actgggccgc gtcggtattg
     5581 gtcttgggct gattttgctg gcgctggagt tgattgtgca ggccgtaacg ccgatcaccc
     5641 aggcaaacgg cgttcaggtg atctttgcct cgctgaccgg cgatattctg ctggatgcgc
     5701 tgattggcgc gatgttcgcc attatcagct actccagcct tgctgctgta ctgctgaccg
     5761 cgactctgac cgctgcaggc attatctcct tccccgtggc gctctgtctg gtgattggag
     5821 ctaacctcgg ttccggcctg ctggcgatgc tcaacaacag tgccgccaat gccgcagccc
     5881 gccgtgtcgc gctgggtagt ctgctgttta agctggtggg tagcctgatt atcctgccgt
     5941 ttgtccattt gctggcagag acaatgggga agctgtcatt gccaaaagcg gaactggtga
     6001 tctatttcca cgtcttctac aaccttgtac gttgcctggt catgctgcca tttgttgacc
     6061 cgatggcacg gttttgcaaa acgattattc gcgatgaacc ggaactggat acccagctac
     6121 ggcctaaaca tctggatgtc agcgcgctgg acacgcccac gcttgctctg gcgaacgccg
     6181 cgcgcgaaac cctgcgcatt ggcgacgcca tggaacagat gatggaaggg ctgaataaag
     6241 tgatgcacgg cgagccacgg caggagaaag agctgcgtaa gctggcagat gatatcaacg
     6301 ttctctacac cgccattaag ctgtatctgg cgcggatgcc aaaagaagag ttggcggagg
     6361 aagagtcgcg ccgctgggcg gagatcatcg aaatgtcgct caaccttgaa caggcctccg
     6421 atatcgtcga gcgcatgggc agcgaaattg ccgataaatc gctggcagca cggcgggcat
     6481 tttcgcttga tgggttgaag gaactggatg cgctctatga gcaattgctc agtaatttaa
     6541 agctggcaat gtcggtgttc ttctctggcg atgtcaccag cgctcgtcgt ttgcgtcgca
     6601 gcaaacatcg ttttcgcatt cttaatcgcc gctattccca cgcccacgtc gatcgcctgc
     6661 atcagcaaaa cgtgcagagc attgaaacca gttcgctaca tttaggctta ctgggagata
     6721 tgcagcgcct gaactcgctg ttttgttcgg tggcttacag tgtgctggaa cagccggatg
     6781 aagatgaagg acgggacgag tattaatatt gtgaatcctg taggcctgat aagacgcgtc
     6841 aagcgtcgca tcaggcacta tgaacaagat gccggatcat gcatccggca attcacttaa
     6901 aaacggtgac cagcttccag cggaaccgct tcttcaccag ccttaaacac ataagtggtg
     6961 ttcgggccac ccagcgtagc gtgaccttta ctcactgtga tccagttacc ttccggcaga
     7021 ccaataatcg tcagttctgg cgcgacgacc agcagttcgc gaatacgctg ctcacgggtt
     7081 tcacctttat ggccttccgg cagcgcgttg gtgaagtgcg ggttgatttg caacgggaac
     7141 agatttagcg catcgaaacc ttgcggatcg acaatcggca tatcgttggt ggtacgaatg
     7201 gttgggcaag caaggttagc gcctgcgctc cagccaatat acagcgcgcc acgtttaacc
     7261 acgtcagtaa ttggagccag cagcccgcgc tcgcggcact gtttcagcaa ctggaaagta
     7321 ttaccgccgc cgacaatcac aatttccgca ttttcaatcg cggcaacggg atcgacaacg
     7381 ctatgaatac cggtgacaga aacacccagc ggagcgagaa ccgcagccgt tttctctgtg
     7441 taatcatccc aggtctgcgt tacgccagcg aaagggataa acaccgctga gcggcgaccc
     7501 tgcaactgtt cagcaattag cggcagtgca tgttccagcc aggctttacc cggcagcgtc
     7561 gagttactca ataaaagcag ttccattact tctccggtta gtaagaaatc acggaaggca
     7621 tgctaccact gcgaatttaa ccacttactg acatggctca cgatgtcacg tattgcaccg
     7681 ggcgctgaga ctgatgacaa acacagcatt gcctgatgcg ctacgcttac ctggcctacc
     7741 agataactgc aatatattgg attttcaaaa ttatgtagac cgaataaggc ttcattccgc
     7801 atccggcata aacaatgtgt actttgccac caatttgagc gccccttaag gcgctgatat
     7861 tttttatcct tctcgtgcaa attgtgctgc ttcagcaatc agccgcacat ccggtcgcga
     7921 agttgctggg gcgaccagcc agcgacatgc agagttaagt agcgcgatta atcatgggaa
     7981 attgcctctg catgggcgag caggtattgc tccacctcac cggaccagat cccctgatga
     8041 cgcgccagaa tcgccgccag tgcttgatcc tgaatctgcg tgagtgacgt cagcggcagg
     8101 tatttcccgg tatacaccag ctttttgccg ccgccgacgg caggcaattc aagcgtggtt
     8161 tcgcccgcgg cattcagccc aagaatatgt gttaccactt ttgcggcctg cacttttttc
     8221 tcttcgatca gtttgaccgc cgcgcgcatg tcgtcggtat tgccgcctga cgtgcccacg
     8281 tagtgggtaa atgcataatg cacatcgtag aaattaattg gcgcgctgaa atgtttatcc
     8341 tgcggtccgg cgaagaaatt caggcaacca tctgtcgcca gcaaggaaga ggcgagagtc
     8401 accagtcctt cattaggcac aaagacgaaa atatcatcga agccgtgacc gccactcagc
     8461 gccattaacg tatcaaatgc tgcatcggtg gcattgagat aatgaatcag tgtttgcggt
     8521 tctgatggat agtgcttgcg cgcataactc aatttgtcgt tgtcggtatc ggtaatgacg
     8581 agcagcgacg ggttaactgg tccatgcagc gcataatcaa tcgccaacag tcccattggt
     8641 ccggtgccgc cgaggatcag cgtgtgccct tgcgggcgaa tccccatcgt gtggttataa
     8701 ctaccttcct gaagatgata gttggcgttg aacgcgccaa tcacgcagga aagcggttca
     8761 accagtgagc cttcaaaata ggtttcgccg tcgtatgcca gcaggcaata ttgttccatg
     8821 acctcgttgg gaataaccac atgcgtcgct tcgccgtcta cccacgggaa ggagtagccg
     8881 gggcaatccg ggcggtcggg gagttgcagg ttggcctgaa tcacataacg ctgacccggc
     8941 tggaatttgt gctgccactt tttacccacg gccagaatat cgccgcaaaa ttcgtggccg
     9001 atgatgattg ggttggtcgt cacatcgtcg ggtacttttt tatggttttc acccagattg
     9061 gcctctttcc aggaagagag gcacaggctg tcagtgacca ccgttgcgag gatttcatcc
     9121 tcctgcattt caggaaggtc aaaggtttcc aggcgtaaat cacgtttacc ataaagacgc
     9181 agagctgttg ttttcataaa ccacctcatg ttgagcgatg gcctcggcag tcgtgccggg
     9241 gcgaagaatc aggacaggaa gcccagagca ttgccgataa tccctaagcc aaacagggcg
     9301 aaaatcagcc ataccgggtt aatttttttg ttgagcagac gaaccattag tagcgtcaga
     9361 ccgagcgcca gcaaaccagg gcaaagttgg tcgagaatgt tctgcacggt catggtgacg
     9421 gtggagccat cggcggcatg cgtttgtgaa accaccaacg gtacgttgat tgacgtccat
     9481 ttggtcacca gcacgcccat cacaaacagt ccgagaatcg acgcgccttc ggtgagtttt
     9541 tgcagcacat tcccgcccat atcgctgacg atattcaccc ctttgcgaaa gccgagctgt
     9601 aggccatacc acttcatcgc cagacgcacc gcgttgaaaa taaagaagaa cagcagcggg
     9661 ccgagaatgt tgcccgaaag tgccagagat gcgccgagcg cggcggtaat cgggcgcagc
     9721 gttccccaga ccagtggatc gccaactcct gccaatggtc ccatcagacc gactttgatg
     9781 ccgttgatgg taccgtcatc aatttccgcg ccgatgaccg ggccacatac ggctggcgtg
     9841 gtattgaaga acaccaggtg tagcctgagc gccgcaacct gatcctcttt taatgggtaa
     9901 agtcgcttga tggcggggat catgtcgtag caaaagccca gcccgtgaat acgttcaaag
     9961 ttaaaggacg cctgttgcag gttggagcgc agaaacatgc tcaccagatc gctgcgtgta
    10021 atttttctct gttccatgat ggctccgtta atcgtcaagt tggtcaaggg cggtggaggc
    10081 ggtggcctgg agttgaggtt cagctttaca ccactgtgga tttagctgga tgtagaccag
    10141 ggccatgatc acgccgacgc caccgaacgc cagcaggctg agatcgaggt agccacctgc
    10201 gaggaaaccg aggaaaaaga aaggcatcaa atatttcacg cccatcatac gtagcaccat
    10261 ggcgtaaccg accaccacga taaaaccgcc agcaatctgt agtcctcggg tcacaaattc
    10321 gggaatggcg tttaacatat tgctgaccat atcggcgctg acgaacagtg agacaatcag
    10381 tgccggaatg gcgacgcgca gcgcctgcac gccaagcgcg gagacatgca gaatatcgag
    10441 agtgcgaaac cgcgcctctt ctgctgcttt atccgccgcg tgctggaaca ctacggtgat
    10501 ggtacgggca aacaccgtca gcacctggcc tgccgcagcc acaggcaacg cgatggcgat
    10561 tccggtggcg atgctttgct gaccaacgat aaccagaatg gcggagatta tgctggcgag
    10621 tgcagaatcc ggagattgcg ccgcgccgac gttcatccag cagagagcta tcaattccag
    10681 cgtaccgccg agtattattc cggtttttaa atcaccgaga attaaaccaa tcaccgtaca
    10741 ggcaattaac gggcgatgag tctgaaattc atccagcacg ctgcccattc cggcaataca
    10801 ggaaaaaaga aatatagcaa tgatttgtag ggtacttatt tccatgattt gtcacctttt
    10861 aagtgtcgtc atccctgcct gttaaagcat atgtacacag gcgctattat ttaatttgcg
    10921 attaactgtt cgtttatttt gtcgataata ttgattgagg gatctgacgc tacgacgcgt
    10981 aaatccagta tgacaccaag attatttaac tcatgaaatg cgttaatatc atcatcatct
    11041 aatgaaaccg ctttggttaa ctgtttttta ccgggccgcc aggccatgcc gccaatattc
    11101 agggtgccta tttttacccc ctggcgaacc atcgctaagg catcctgtgg gcgagtgaaa
    11161 agataaaaaa cggtttcgtc ctgatattgc gggttgtgat aaaccgcgac ggctttttca
    11221 atattgacca cgttaacctt cattcccggt ggcgcagcct gacgcaataa tgtccggcgc
    11281 acttcatcgt tataaacttc gtcattgcag ataataatgc gctgggcgtt agccactttc
    11341 gaccagacgg tagtgacctg gccgtggatc agacggtcat caatgcgggc gagcgtaatg
    11401 ttcatcagaa atcctcctct gtcgttgcca gttgtttcca gacaacgcag gtttgctttg
    11461 cgatttgcgt caggtgttca cacagttcat caacattcat gctgtcctgg ttatcaacca
    11521 gttcgagcgc cagcggcaga gaaaggccgc tgattacccg caaacgagga ttgcgcatcg
    11581 caagcattgc tgccgcattc cacgggctgc cgcactgtaa atccacggca atcagccact
    11641 catcttgatt gtgaatgctt actaactttt ccagctttac cgcaatgtct ccggcgtttt
    11701 caccgggcac aaatgcaacg gcttcaacat tggcgttgcc gtaaaccatc tgaaccgatt
    11761 ccagcatggc gcaagctaat ttgccgtggg cacaaaaaat ggcgttgacc ataaagcctc
    11821 cttagccgcg cgttttgccg cccgcaatgt tagtggttac tccggtgatg tagctggcgc
    11881 gttcagacaa cagataacaa acaaaatcag ctacttctgc taatcttccg gcgcgcccaa
    11941 taggaatggc gtttttggta tagccttcac gcagctgctc gacggtgata ttgcgcgtcc
    12001 acgccagcgc ctcttcatat tccggcgtgc gtagcccggt tttttccaga atcccgggcg
    12061 cgataccgac cacacggata ccgtgtttac ccagctcttt cgaccaggag cgcgtgaagc
    12121 tattgagcgc ggctttggta gcggcgtaac agctttgtcc ttctgagcct tccagcccac
    12181 tttctgagga aacattcaca atcacgccat catgttgttt gaccatctgt cgcgccaccg
    12241 cctgcgacat cagaaaaaca cctttctggt tgatattgac cattttttcg aatgcagctt
    12301 cgttgagttc atactgcccg gcaggcgctt tctcatcgac cagcagacgc gggaaattga
    12361 ccccggcgtt attgaccaga ccgtcgatgc gaccaaaacg ctggataatt tccgctaccg
    12421 tatgatttac ctcttttgtg ctagaaatat cggtcggcca aaactgatag cctttatgac
    12481 cttcatattg accatcgcca ccgtgaatat cgaccatctg tacattcgcg ccttgtgcta
    12541 ataattcctc tacaatcgcc agaccaatac cggatgcgcc gccggtgaca ataatgattt
    12601 tatcctgcaa atttaaccac gtttgcataa cagactcctg attttagata atagggaccc
    12661 gccgtaattt ttattacggt aaacatcggg aagattttta tttcagtaat agttcagctg
    12721 tgctgctatt cgttaccaga caattaatat attttccacg cagtgcacca ataattccgc
    12781 tgtatttttc ttcactcatg gcaatgccaa tggaatatcg cgcctgcttt aatttattca
    12841 tttcgataga gagcgttttt tcgctcatat tcgtttcaac cattgcgccg tgaatatcaa
    12901 aaaagcgcga gcaaatatcg ccagcaacct ggcgggcatt caggtcgtca ctctcttcac
    12961 caccataaaa cgcatgccag ttagcgccgt cgcgaatggc cggtgagcca attcccacca
    13021 gggcgatatc cagattatcc cagtaggcag agatggtttt aaagtgctga gactgcatga
    13081 tcccattacg aattaatggg ttatccagca gagccggaaa atccgcgaga tgcgattccc
    13141 ctttcagctt cgctgccgcg ctgtaggtta atgtgtttac atgatagcgg ctttcgagtt
    13201 taccggacgg tccgccaata attggtacgc agattaactg ccgcgattgc cccgcctgcg
    13261 gcaagttttc aaccagtgcg ctcaccgcgc gaccccagga aaaaccgaca atatcgccag
    13321 gctccagcaa gcgatccagc agttgcgcgc cgtgtaaccc catcatcgcc agttgagtct
    13381 cttcatcctc atcatttccc gacaccacca caacgtcttt caggccaaac ttttgcttca
    13441 gttgctgctc cagccagaga ttttcgttgt agtcatagtt gatggcgatg gtgaccattc
    13501 cctgatcgcg gcctcgttta agcaagcggc tgatggtggt gcggtaaata cccagttcgc
    13561 gcgcgatttg cgcctgcgtc atatcctgtt cgtaatagag ttgggcaatc ttcacaatca
    13621 aacggatatc gtcactgttt tccattgccg cctcctggtt ctgcacattt gtgcaaatac
    13681 gctttctcac cgcttgatct gatataaccc catcatgagg gcctttcaaa gcgatttttc
    13741 actctttgca tggggagaaa tagcgctttc tcttgcacat ttgtttctct gcgtagtagt
    13801 catcagcaca ggtgtgcagc gattcgtaat atgttgatta tccttatttt gccaggcaaa
    13861 tttgcatcgt acttccggag acacttttgt gataaaaggc gtggcagatc acatttaatt
    13921 tcgacgttgc cgcataaccc tgtttttccc cgctaagagg tacggtatat tccgcgtttt
    13981 acaggagaat ttatgctgcc cgactcatca gtccgtttaa ataaatacat cagcgaaagc
    14041 ggaatttgct cacgccgcga agcggatcgc tatatcgagc aaggcaatgt gttccttaat
    14101 ggcaagcgag ccaccattgg cgatcaggtg aaacccggcg acattgtgaa agtaaacggt
    14161 cagttgattg agcctcggga agccgaagat ttggtactta tcgccctgaa caagcccgtc
    14221 ggtattgtca gcaccaccga agatggcgag cgcgataaca ttgtcgattt cgttaaccac
    14281 agcaaacgcg tgttcccgat tggccgcctg gataaagact cccaggggct gattttcctc
    14341 accaatcacg gcgatctggt gaataagatc ctccgtgcgg gtaacgatca tgagaaagag
    14401 tatctggtga cggtcgataa accgattacc gatgaattta ttcgtggtat gggggcgggg
    14461 gtgccgatcc tcgggacggt gacgaaaaag tgcaaagtca aaaaagaagc gccgtttgtc
    14521 ttccgcatta ccctagtgca ggggctgaac cgtcagatcc ggcgcatgtg cgagcatttc
    14581 ggctatgaag tgaaaaagct ggaacgtacg cgcattatga acgtcagctt aagcggcatt
    14641 ccgctggggg agtggcgcga tttaaccgac gatgagttaa tcgacctctt taagctcatc
    14701 gaaaattcct cttccgaggc aaaacctaaa gcgaaggcca aaccgaaaac agcgggcatc
    14761 aaacgtccag tcgttaagat ggaaaaaacg gcggaaaaag gcggtcgccc ggcgtccaac
    14821 ggtaagcgtt ttacctcgcc ggggcgtaaa aagaagggac gctgattaac gcctcagact
    14881 gatgaaaacg tccaaaaaga gctcggacag tcccctcgcc ccttcgggga gagggttagg
    14941 gtgaggggaa gattgtgtcc acacagacga gtgaaaatct caggcgttga ttgttcatta
    15001 gcgcctgccg cgcgtcgacg tatttgctga ccagctaaat gacgcttcag gatccggttt
    15061 ataagcctgc tttttcttca attggcgggc ttttttcgct tcagcctcac gctcgaccat
    15121 taatttatcg atgtactctt tcttaatgct gttggtttcg gagttggtca gtacg
//
LOCUS       DAZSNO010000088        15139 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZSNO010000088 DAZSNO010000000
VERSION     DAZSNO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15139
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.088"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(16..336)
                     /gene="chbB"
                     /locus_tag="ACHWN2_002618"
     CDS             complement(16..336)
                     /gene="chbB"
                     /locus_tag="ACHWN2_002618"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR8932101.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(635..973)
                     /gene="osmE"
                     /locus_tag="ACHWN2_002619"
     CDS             complement(635..973)
                     /gene="osmE"
                     /locus_tag="ACHWN2_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR8932102.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1175..2002
                     /gene="nadE"
                     /locus_tag="ACHWN2_002620"
     CDS             1175..2002
                     /gene="nadE"
                     /locus_tag="ACHWN2_002620"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR8932103.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            2232..3119
                     /gene="cho"
                     /locus_tag="ACHWN2_002621"
     CDS             2232..3119
                     /gene="cho"
                     /locus_tag="ACHWN2_002621"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR8932104.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3079..3654)
                     /gene="ves"
                     /locus_tag="ACHWN2_002622"
     CDS             complement(3079..3654)
                     /gene="ves"
                     /locus_tag="ACHWN2_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR8932105.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3857..4342)
                     /gene="spy"
                     /locus_tag="ACHWN2_002623"
     CDS             complement(3857..4342)
                     /gene="spy"
                     /locus_tag="ACHWN2_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR8932106.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4672..5640)
                     /gene="astE"
                     /locus_tag="ACHWN2_002624"
     CDS             complement(4672..5640)
                     /gene="astE"
                     /locus_tag="ACHWN2_002624"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR8932107.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5633..6966)
                     /gene="astB"
                     /locus_tag="ACHWN2_002625"
                     /pseudo
     CDS             complement(5633..6966)
                     /gene="astB"
                     /locus_tag="ACHWN2_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(6963..8441)
                     /gene="astD"
                     /locus_tag="ACHWN2_002626"
     CDS             complement(6963..8441)
                     /gene="astD"
                     /locus_tag="ACHWN2_002626"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFR8932108.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8438..9472)
                     /gene="astA"
                     /locus_tag="ACHWN2_002627"
     CDS             complement(8438..9472)
                     /gene="astA"
                     /locus_tag="ACHWN2_002627"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR8932109.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9469..10689)
                     /gene="astC"
                     /locus_tag="ACHWN2_002628"
                     /pseudo
     CDS             complement(9469..10689)
                     /gene="astC"
                     /locus_tag="ACHWN2_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            11135..11941
                     /gene="xthA"
                     /locus_tag="ACHWN2_002629"
     CDS             11135..11941
                     /gene="xthA"
                     /locus_tag="ACHWN2_002629"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR8932110.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12108..12818
                     /locus_tag="ACHWN2_002630"
     CDS             12108..12818
                     /locus_tag="ACHWN2_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR8932111.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12823..13500
                     /gene="ydjY"
                     /locus_tag="ACHWN2_002631"
     CDS             12823..13500
                     /gene="ydjY"
                     /locus_tag="ACHWN2_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR8932112.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13515..14222
                     /locus_tag="ACHWN2_002632"
     CDS             13515..14222
                     /locus_tag="ACHWN2_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR8932113.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            14222..14770
                     /locus_tag="ACHWN2_002633"
     CDS             14222..14770
                     /locus_tag="ACHWN2_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR8932114.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14780..15106
                     /locus_tag="ACHWN2_002634"
                     /pseudo
     CDS             14780..15106
                     /locus_tag="ACHWN2_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         3659 a         4048 c         3770 g         3662 t
ORIGIN      
        1 aaaatttaaa ataaattaat ttgctgcggc ttttttaatc gctgcaaccg cagccttaag
       61 cacgcctaaa ccatcgactt tgccataaag cagcgagtca attacttcaa ccggtttgtt
      121 gggtaacaaa cgctggattt cgggcaacat ataagcaatc tgcggcccta ataacacgac
      181 atcggcattc tgaccttttt caccagccag tgtttccgga aatgcttcaa taatgaccgg
      241 aacttcatat ttttctgcct gtgcgcgcat ttttgatacc agtaaagaga tagacatgcc
      301 cgcagaacaa aacagataaa tgtgtttctt ttccataaaa ctgccctcat cgacgattat
      361 ctgtcagcca gacactccgc aagccttgac ctgcttccat gctctgggta acttgcgaaa
      421 ccaacatgac tttttttgtg gctgaaagga gtatacggta tagacctgag caatagtatt
      481 tctttgacct tataaattgt ttctccttga cctgtggtta tgaccctctt cacatttcgg
      541 gcaaataatt cgcgaagata attaagaact caggccaaaa aaaaccggcg caatggccgg
      601 tttccgttgt tactcaagct ttcagacgaa ttgattactt cgcagcctgt ggatcagtgt
      661 cgtattcagc acaggtctga taaccggagt tgatgacatg tccggtatca tctaacgcga
      721 caaagtaggt ttctgcttta ccatcacgtt gacccaggat gtaggtctgg caagtaccgc
      781 gagcatggat catgctcact tcagacgaag gtttacccgc aatttgtgca acctgcgccc
      841 ggctcatgcc ttttttgacg tctttcacca caggctgtac aaactggtct ttggtacgat
      901 cataagctgt acaacccgcc agcatggtta ataccgccgc tgcactcaga attcctgcca
      961 tattcttgtt catattccgt cctcttgttt atcagcgtgt tagataagcc tggaatacat
     1021 tgggcgcttt ttcaagcccg tgaacgaaac ggctccgctt tcagaggatt cctgtatgac
     1081 gttttaacca ccattcagcc cgctgtcgct tgtcgtttca gtagcaacgg gttagcttta
     1141 aggaagtttt gtcttttctg tctggagggg ttcaatgaca ttgcaacaac aaataataaa
     1201 ggcgctgggc gcaaaaccgc agattaatgc tgaagaggaa attcgtcgta gtgtcgattt
     1261 tctgaaaagc tacctgcaaa cttatccgtt cattaaatca ctggtgctcg ggatcagcgg
     1321 cggtcaggac tccacgcttg ccggaaagct gtgccagatg gcgattaatg agctgcgcca
     1381 ggaaactcgc aacgaatcac tgcaatttat tgccgtacgc ctgccctatg gtgttcaggc
     1441 tgacgaacaa gattgccagg atgccattgc ctttattcaa ccggatcgcg tattaaccgt
     1501 taatatcaag ggcgcggtat tggctagcga gcaggcattg cgggaagcag gcattgaact
     1561 gagcgatttt gtccgtggca atgaaaaagc gcgtgagcgg atgaaagcac aatatagcat
     1621 tgcgggtatg accagcggtg tcgtggtggg caccgatcat gcagcagaag ccattaccgg
     1681 attcttcact aaatatggtg acggcggtac ggatattaat ccgctgtatc gtctcaacaa
     1741 acgccagggt aaacagttac tgacggcatt aggttgcccg gaacaccttt ataagaaagc
     1801 cccaactgcc gatcttgaag atgatcgccc ttctctgcca gatgaagtgg cactcggcgt
     1861 gacctatgac aatatcgacg actatctgga agggaaaaac ctacctgaac aggtcgccag
     1921 aacaatagag aactggtatc tgaaaaccga acataaacgc cgtccgccaa ttaacgtttt
     1981 cgatgatttc tggaaaaagt aataatttgc acctattgga ttgtgcgaaa aagagtaatt
     2041 tgttcacgcc ggatgcggcg tgaacgcctt attctaccta caaaactatg caaattcaat
     2101 acattgcagg agtcgaatag gcctgacagg cgtagcacgt cagacggtgt aacctttgtc
     2161 atcaacccgc ctctttttta atcgcctccc gcctgttaca ctggatagat aaccagcatt
     2221 cggagtcaac agtggtacgg cgtttaactt ctccgcggct cgaatttgaa gctgcggcaa
     2281 tttatgaata tcccgaacat ttacattcat tccttaatga cttacccacc cgaccagggg
     2341 tgtatctgtt tcatggcgaa agtgacacca tgccgctcta tatcggcaaa agcgttaata
     2401 tccgcagtcg ggtgctttct catttacgca ccccggatga agccgccatg ctacggcaat
     2461 cccggcgaat tagctggatc tgcaccgcag gcgaaatcgg cgctctgctc cttgaagcgc
     2521 gattaatcaa agaacaacag ccgctgttta ataaacggtt gcgccgcaat cgccagctct
     2581 gtgccctgca attaaatgaa aagcgcgtcg atgtggtgta tgccaaagag gtggattttt
     2641 cacgagcccc caacctgttt ggcctgtttg ccaataggcg cgcagctttg caagcattgc
     2701 agagcatcgc tgatgaacaa aaactttgtt atggcctgtt gggactggaa ccgttaagtc
     2761 gcggtcgtgc atgttttcgt tcagcgctaa aacgttgcgc cggagcatgc tgcggtaaag
     2821 agagccatga ggaacatgcg ctacgcttgc gccagtctct ggagcgtttg cgagtggtgt
     2881 gttggccttg gcaaggtgcg gtggcactga aagaacagca cccggaaatg actcaatatc
     2941 atattattca aaactggctg tggctggggg cggttaattc gctggaagaa gcgacaacgt
     3001 taattcggac acccgccggg tttgatcacg acggttataa aattctttgt aagccgctgc
     3061 tttccggtaa ctatgaaatt actgaacttg atccggcgaa tgaccagcga gccagttgat
     3121 ttcactgaac aacagtttac cttgcggttg taaaagccgc agggtgtgcc tgccatcgaa
     3181 ccagcacacg ccctggtcgg tagtgagcag tttgtcgccc agttgccacg ccccgttaat
     3241 cacaaacacc accccgccgc gtgagccaaa ggtagtaaac gtacgttcgg caattctgac
     3301 tttcgcctta cagacatcca ggcgcgtcat aatattgaaa tccatcgaca tctgccctgc
     3361 cgtcagtttc gcttttacca cctggtccgc cgtaaaggca aaaggctgta acggttttaa
     3421 ggtatggtta aagcggtctg cgctttcaag gagcatctcg ccgccttcca gcaacgtcac
     3481 tatcctttcc atgccgggaa acaaagaaaa ctccccattc gccgcgatgg atgcaatgct
     3541 ggcacgccag taaaaatcac gttttgccgg tgggaaagtg caaatttcgc gcgtttcacc
     3601 ggcagcgttt cgccacagat tcaccgacat tttacgcata tcaaagtatt ccatactcgc
     3661 tcccggcccg ttcttttcat tgttatcgtt caggtaaccg tggatggtta tcggcaactc
     3721 gggtgattta cgttagtggt gatcaggctt tttttacaac tgtttgagaa gagaaaagaa
     3781 aaccgccgat cctgtccacc gcattactgc aaggtagtgg acaagaccgg cggtcttaag
     3841 ttttttggct gaaagattat tcagcagttg caggcatttt accttttgcc gctggacgtt
     3901 ctgtcagacg cttctcaaaa ttagcattaa attgcttttt ctgttccggc gtcaggatgt
     3961 tgtaaatttt gttctgggtt tccatgtgcg ccagcatgtt agctttgcgc tgttcttcca
     4021 tttttgcgat ctgcgcttca gcttttgctt tatcgaaggt atcgctggca atgatgtcat
     4081 gcattgcgcg gcgttcttcc agcggcggac gtttcatctg gtcacgctgg cctttcatga
     4141 tttcgtggat ctgctgtttc tgcgcgtcgg tcaggttcag gtctttgaac atcatgtcct
     4201 gatgcggacc gaacttgcct ttgtggtgca tcatcggctt cgcgtcagcc ggtgctgcgg
     4261 tagtggtgtc tgcggcatgg gccaggttag ccgcgccaag agccagggta gaggcaacaa
     4321 acagtgcagt taatttacgc atattctata tccttccttt cagttattta ttacggcttt
     4381 cttaagtagc gtgccgtgtt gacgagatta actttactga ctttagcgtc aattaatcag
     4441 agcaacggta aaacaatgaa agtgtaaaaa acacttttgc gccaattatg gagaaaaaaa
     4501 gaaaatttga tggagagtga tgagagaata ttacaacacg atgattttgc agagattatg
     4561 aagaaccata ccggatgact ggtgataaat aaagcaaata accaggatta atctgtatta
     4621 atttataaga aagcaactta atacccgcag aatgatttct gcgggtaagt attagcttat
     4681 tttttcgagc attaatcccg cgcgtaatcc caacgctacc aacggattag ggaataacac
     4741 atactctaca tcatgggtta cggtaaaacg ttcctctccg tcctgcgcca gcaatgttcc
     4801 tttctcaaac ggcataaaat tcagcgtgtc acttgccata tgcatttcga aggacggcga
     4861 gtggcgagta atttgcgaaa ccacccgata acggagcggc ggtgttctca cgataccgac
     4921 actctcacca gatagcagcg cagcaattgc gctggcagtt actgcaaact ggcgaagatc
     4981 gttttgccca aagggcaacg ctttgccaag ttccaacgta caggccagcg cgccaaaatg
     5041 tctgacgctg aaatgggtaa acgtaccacc aggctcctga tggaacacca gcgcctccag
     5101 ccccgccgca cccagccacg tcagaaattt ctcgtcccag ggaatgtcgc gttgcggtaa
     5161 tacaccgaac tgcggatgca aggagccacg aattgcggta tgtagatcaa ggtgccaacg
     5221 cacagattct ttgccctggt cataaaaatc ttccaggcac tgttccagtt cgcgcgcccg
     5281 acaggtttct ccgctttcag caaatagctg ccaacgaccg ccaaacattc ggttcatatc
     5341 gctatggcaa taacgtttcc cttgcttcag cgcaggagga ttcccgagga tcaccagcaa
     5401 ccgccaacgt aacgggatct cgccgtgaga tatcgcgcca agcaacgcgt ccagcatttc
     5461 cacaggtgcc gtctcattac cgtggatccc cgcagaaatc accagcgcgc cttgcggtgg
     5521 cgttaatggc gtcagttcca gcacaccatc gcccagccag cgccagcgaa cgccgttgat
     5581 ttctcgctcg gtgataaccg gttttttacc cgttaaggtc agagcaagaa aattatccat
     5641 tgccccctcc ctcgcgctgg aacggataaa ccgaaccgag attcagtaat tggctcaata
     5701 catccagtgc ttcccgccct tcgcgcagca attgcgggtc ggccagatcg gcagcagtaa
     5761 gacgatcgcg gtagtaacga tccacccagt cattgagcgc attaaacaag gtatcgttca
     5821 tcatcaccgc cggattcacc gcccggcgtt cttcttctgt caataccacc cgcaaccgca
     5881 ggcacgccgg gccgccgcca ttcgccatgc tttcacgtaa gtcaaagact tttagttcgc
     5941 taatcgggtt gtcagcggca aggagttcat tgagataacc ccatactccg gcgtgttccc
     6001 gacactcctg aggcagcacc aacatcatgg aaccatcatc gcggctcaac agttggctgt
     6061 taaacagata ggtagacacc gcatccgaca cggaaacctg agttgccgga acttctatcg
     6121 ccataaaacc gttcacccgc gtgccagtaa ctgtgactgg cgagcgaacg cctgttggtg
     6181 gcaaaacagc acctggcggt tactcacggc aatcacgtca ttatgaaaaa cgctctggtc
     6241 gataacgtcc gggttttgct gggcgaaaat cacctgttgg ggattcacct gattaagcct
     6301 tgccaccgcc tcgctggctt cgcgagtctg tcgcgccgga taacgggaag gccgggtatc
     6361 attgccttct tctcgcccgt agacaaaaag tagcataccc ggttcaccgt aatgaccgcc
     6421 gagacgattg tggtttgccg ccccctcatc accgagcaac gctacctgcg gcaacgccga
     6481 atggacgcta aatttctctt cgtccttaaa aatcgctttt aacagcgatt cagtgacggg
     6541 cgcttccagc gaacggtgaa atttattgtt caggttggca acggtgagat gcactttgcc
     6601 atccagcgta tcggcagatg gcgcgatcgt tgccgcattg gctacccaca ttggcgaagc
     6661 ggaactgacg ctggaaagcc agtgcggtgc ctggcgtgca actttttcca gtacctgctc
     6721 atcgctacca ctgaatccca actgacgcag caccggaata aacggacgct cgtgcggcgg
     6781 aatcacggcc tgggggaatc ccgcatcggc aagggctttc attttcagta agccctgttt
     6841 tgccgccagt cgcgggttag acacctgaaa acggtgacgg gtagaggctt cattaccaaa
     6901 cgacaggccc gcgtaatgat gcgtcagccc taccagcccg tcgaaattaa cttcccaggc
     6961 gttcatcgca ccacctcatc ggaaaaatcc agcccggggt taagcgtggc gggcaatgtt
     7021 aacgagtccg actccaggct cgccataggc catgcgcagt aatctgcggc ataccaggcg
     7081 ctggggcgat ggttaccgga agcaccaatg ccgccgaatg gggcggtact ggcagcacca
     7141 gtaagcggtt tgttccagtt aacaatcccc gcccgcgcct ccagcaacag ttgatcgaat
     7201 ttttcccgct cgggggaaac cagaccgcaa aagagtccga agcgagtgtt gttcgccatc
     7261 cgaatcgcct catcaaaagt atcataacgc cagacgcgca aaaacggccc gaacacctct
     7321 tcatccggca caccgccaac gcccgtcatt tcaatgatcc ctggtgtcag caacgatgtc
     7381 cctgcttgta ataagcgcgg cgcaagcagg gttcgtccgc ccatcgcttc cagttgctgc
     7441 caggcagtaa ccacctgctg tgcggcctgt tcagaaatca gcccgccaat aaacggctgc
     7501 ggttcgtcat cccagttgcc cggcgttaat cgctggctga cggcaaccag actagcaaga
     7561 aacgcatcgc cctgcgctcc gcttttgagc aataaacggc gggcgcaggt gcagcgttgc
     7621 ccggctgtga caaacgccga ctgaatggtc agatggacag ccgcgtcgat atccgccacc
     7681 tcatcgataa ttagcggatt attaccgccc atctcaaggg cgagaatttt ctccggctga
     7741 ccggagagct ggcgatgcag ctggtagccg gtattggcgc taccggtaaa cagcaaaccg
     7801 tcgagatcct ccagcgcact cagcgcctga cccgtttcac gcccgccctg caccaggttc
     7861 agcacgcccg gcggcaagcc agcctgctgc cataaacgca ttaccgcttc gccactccac
     7921 ggtgtcagtt cgctgggttt aaagataatg gtgttacctg ccagcaatgc cggaacgata
     7981 tgtccgttcg gcaaatgacc agggaaatta tacggcccaa acaccgccag cacgccgtgc
     8041 gggcgatgtc gcaggctcgc cgcaccgtcc ggcatttcac tacgctgctc gccggtacga
     8101 acgtgatacg ccttaattga tatcgcgatt ttattgatca tcgccgtcac ttcggttgcc
     8161 gcttcccagc gcggcttacc cgtttctctg gcaataatcg cggttaattc ggctttattg
     8221 ctttccagca atccggcaaa gcgttcgaca acgacctgac gttcagccaa tgagagccgc
     8281 gcccagcgcg gaaacgccgc acgggctgcc cgacaagcct gttcgacctg agcggcatcg
     8341 gcatcattgc cttgccataa cacctcgccc gataccggat tacgcttcac acgcgatgcg
     8401 ccctggcccg ttatccagtc accgttaatc cataaagtca tgctgttttc tcctctgcgc
     8461 acaggcgcac cagacgaacg cgatcccctg cgtggcattt gagggcatcc agttgtgcgg
     8521 cggttaaaat caaacgctcg gttgccggat cggtacgcgc cagcaccacg cggaaatggt
     8581 gataattttc attggcgacc aggcaggccg gaaaatcgcc ctgcgcaggc tgcccttctg
     8641 ccacttccac cagccgactt ttacggatgg cgcgcacgcg gtcgatgtca cactcaagcg
     8701 ttggcccgcc gtcgaagatg tcgatatagt tgcggtaacg aaaaccttct ttctccagca
     8761 ccgcgcgggc aggcgcggtt tgcggatgta cctgaccgat gacgtcctgg gcttcctggg
     8821 ataaaaagtg ggtatagatc ggatgtttcg gcatcagttc tgcaataaat gccttttgcc
     8881 cggtgccgca gagaaaatcg gcgcggctaa aatccatcga aaagaagcgt ttaccgaggc
     8941 tttgccagaa cggtgaatag ccgtgttcgt caatcacccc gcgcatttca gcaaccactt
     9001 tgtcattaaa cttgtcgcga aaagccgcca taaacataaa gcgcgatttc gatagcaaat
     9061 agccgttgcc ctctttgcgc cagtccgggt cgagaaacag cgtgcacagc tcgctgctgc
     9121 cggtgtgatc gttactaaga aacagcgtcg gcaatgcgtt atagacattc agctcttttg
     9181 aggcgtgaac caacgtgccg acgcgatagt tgtaccaggg atcgttcagc ccaaccgcca
     9241 cctcaatagc gcaaatcccc gccacggtgc ctgtctcgct atcttccagc acgaacacat
     9301 agccctgctc gcttttgggc agttcacctt gccaggtttt gattgccctt tcgatacgcg
     9361 ccgaaagcgt ggcttcattg gcgggaagcg acgtcaggcc gccgcccgtt ttgctggcaa
     9421 gctgcatcag cgccgagaca tctgagcgct caacgggacg gatgaccatc atgatgaacc
     9481 tcggctaaca aagtgttcgc aagcagctgc aaagcgatcc agtccggtcg tcacctcttc
     9541 ttcgctgaca ttgagcgcag gcgcaaaacg caccacgttg ccacccgcaa tcagtaccat
     9601 cacgcctgct ttcgccgctt ccagagagat ctgtttcgct tgcccggcgt aatcggcatt
     9661 cagtacacag ccaatcaaca aacctaagcc gcgaacttca ctgaacaaac catagcggtg
     9721 attaatagta ttaagacgct caacaaacca gtcgtgacgc tgtttaacgc cattaagcat
     9781 ctctggtgtg ttgatgagct ccagcacttt gcctgctacc gccgaggcca gcggattacc
     9841 gccataggtg gtgccatgag tgccaacggt catcacgcgg gcgcactctt cggttgccaa
     9901 caacgcaccg accgggaaac cgccgcccag cgctttggcg gtggttaaca gatcgggcgt
     9961 cacgccgtag tgcatatagg catacagctc accagtgcgc ccgacgccgg tttgtacctc
    10021 atcaaaaatc agcaaggcat tgtggcggtc acacaattca cgcagacctt gtaaaaacgc
    10081 gttgctggct ggcaccacac cgccttcgcc ctagatgggc tcgacaatca ccgcacaggt
    10141 ggcgtcgtca atcagcgcgc tggcagagtt aatatcgtta tatgcagcat gacgaatatc
    10201 cgccggcagt ggcgcaaaat cctgtgaata ggctggctgc ccacccgcac tgacagtaaa
    10261 cagcgtgcga ccatgaaacg catttttgaa cgccacgatg ccactcttat ggctgccgta
    10321 gcggtcgtga gcgaatttac gcgccagttt tagcgccgct tcgttggctt ccgcaccgga
    10381 gttacaaaag aagacgcgat cggcaaacgt ggcgtcgatc aattttttcg ccagtcgcag
    10441 taccggctcg ttggtgtaac cgttgccggt atgccagaac ttactcgcct gttcgttcag
    10501 cgcttcacgc agttccggat gcgcatggcc cagcgcgttc accgcaatgc cacccgcgaa
    10561 gtcgatatac tctttcccct gctgatccca caagcgcgaa ccttcgccac gtaccggtat
    10621 aaagggtgcc ggagcgtaaa caggtatcat ccattcatca aagttttcac gcgtaattgg
    10681 ctgagacata gcgacctcta cagtaaataa ttcgttattt gtatgttaac aataagtaat
    10741 gtttgcgctg taaatgtaga ttgcagggtt cgtgccagcc agtgataaaa gtgcataaac
    10801 ggcggaggct aactggaaat caaggagtta taaccaaacc atatgcattt aaagtgcata
    10861 taaagtgaat acgtttgcga tgtgggtgaa taaaaagaat aaaaaacgca atgttatgca
    10921 gaagtaaaat ataattctag aattgtgatc attgacgaaa tttactggaa attactgcgc
    10981 cattctgacg cagcgcgcac caaaagcggg cattttttgc gccatcgttg acatcattaa
    11041 caaccatcga tcaaatcact taacaacagg cggtaagcaa cgcgaaattc tgctaccatc
    11101 cacgcactct ttatctgaat aaatggcagc gactatgaaa tttgtctctt ttaatatcaa
    11161 cggcctgcgc gccagacctc accagcttga agccatcgtc gaaaagcacc aaccggatgt
    11221 gattggcctg caggagacaa aagttcatga cgatatgttt ccgctcgaag aggtggcgaa
    11281 gctcggctac aacgtgtttt atcacgggca gaaaggccat tatggcgtgg cgctgctgac
    11341 caaagagacg ccgattgccg tgcgtcgcgg ctttcccggt gacgacgaag aggcgcagcg
    11401 gcggattatt atggcggaaa tcccctcact gctgggtaat gtcaccgtga tcaacggtta
    11461 cttcccgcag ggtgaaagcc gcgaccatcc gataaaattc ccggcaaaag cgcagtttta
    11521 tcagaatctg caaaactacc tggaaaccga actcaaacgt gataatccgg tactgattat
    11581 gggcgatatg aatatcagcc ctacagatct ggatatcggc attggcgaag aaaaccgtaa
    11641 gcgctggctg cgtaccggta aatgctcttt cctgccggaa gagcgcgaat ggatggacag
    11701 gctgatgagc tggggggtgg tcgatacctt ccgccatgct aatccgcaaa cagcagatcg
    11761 tttctcatgg tttgattacc gctcaaaagg ttttgacgat aaccgtggtc tgcgcatcga
    11821 cctgctgctc gccagccaac cgctggcaga atgttgcgta gaaaccggca tcgactatga
    11881 aattcgcagc atggaaaaac cgtccgatca cgcccccgtc tgggcgacct tccgccgcta
    11941 atttagcagc tctcctggct caaactgggt caggagaatt aaccttgaga aaaatcaaca
    12001 aactgtcagt aatgatttgt tgcctgccgt cctttgttat accgtcactg cgtttttagt
    12061 tgtctgacca cttctctatt atcaagtttg atataggaaa ctccacgatg aacgctgagc
    12121 gtaactttct gtttgcctgt cttatttttg cgctggtcat ttacgctatc cacgctttcg
    12181 gtttattcga tctgctcacc gatttacccc acttacagac actcattcgc cagagcggat
    12241 tgttcggtta tagcctctat attctgttat tcatcaatgc ccccctcttt ctgttaccag
    12301 gaagcatact ggtgatcgct ggaggaatag tttttggccc gctcttaggg acacaactct
    12361 cattaattgc cgcaacgctg gcctcctcgt gctcattcct gctggcgcgc tggctgggac
    12421 gtgatttact gctgaaatac gtgggccata gccatacctt ccaggccatt gaaaaaggca
    12481 ttgcgcgtaa cggtattgat tttcttattc tgacccgctt aatcccgttg tttccttaca
    12541 atattcaaaa ttacgcttac ggattcacca ccatcgcctt ctggccttat acccttattt
    12601 cggcactcac gaccctgccc ggtattgtta tttataccgt gatggcaagc gatctcgcca
    12661 atgaaggcat tacgctgcgc tttattttac aactctgtct ggcgggcctg gcgctgttta
    12721 ttctcatcca gctcgcaaaa ctctacgccc gacacaaaca tgtggatctg tctgcttcgc
    12781 gccgcagccc acttactcac ccaaaaaatg aaggatagaa cgatgtcgca acattattca
    12841 gtgtcatgga aaaaaggact ggctgcactc tgcttactgg ctgttgccgg gcttagcggc
    12901 tgcgatcaaa aagagaatgc ggcggcaaaa gtggaatacg acggactttc gaacagccaa
    12961 ccgctgcgcg tcgatgccaa taaccatacg gtaaccatgc tggtgcaaat taatggtcgt
    13021 ttcctcaccg acgacactcg tcacggtatt gtgtttaaag atggctccaa cggacataaa
    13081 tcgctgttta tggcttatgc gcccccgaaa gcattttatg aagccctgaa agaggcaggt
    13141 ggtacgccgg gcgaaaacat gacgatggat aataaagaaa cgactcatgt cacaggcagc
    13201 aaactggata tttcggtcaa ctggcaaggg gcggcaaaag cgtattcctt cgatgaagtg
    13261 attgttgaca gtaatggcaa gaaactggac atgcgctttg gcggtaattt aacggcagca
    13321 gaagagaaga aaacgggttg cctggtgtgt ctggatagct gcccggtcgg catcgtcagc
    13381 aatgcaacat acacttatgg tgcggtggaa aaacacggcg aagtgaaatt caaaggcaat
    13441 gcctcagttc tcccggcgga taacacgctg gcaacggtta cctttaaaat caccgaataa
    13501 agccaagata aaggatgatg aagatacaat cgcgtaaaat ctggtactac cgtataaccc
    13561 tcatcatcct gttgttcgct atgctgctcg catgggcgct gcttcccggc gtccatgagt
    13621 ttatcaatcg cagcgttgcg gcgtttgccg ccgtggacca acagggtata gaacgcttta
    13681 ttcagtctta cggcgcactg gcggcagttg tctcgttctt gttgatgatt ttacaggcca
    13741 ttgctgcacc gctacctgcg tttttgatca cctttgccaa tgcgtcgctg tttggcgcgt
    13801 tctggggcgg cttgctgtcg tggaccagtt cgatggccgg cgcggcgctg tgctttttta
    13861 tcgccagagt gatgggccgc gaagtggtgg aaaaattaac cggcaaaacc gtgcttgaca
    13921 gtatggacgg ctttttcact cgctacggca aacacaccat cctggtctgt cggttattgc
    13981 cttttgtccc tttcgatcca atcagctatg ctgccggtct gacttcaata cgttttcgct
    14041 cgttttttat cgccaccggg cttggtcaat taccggcgac tattgtttat tcctgggcag
    14101 gcagcatgtt aacaggcggt actttctggt ttgtcaccga actgtttatt ctgtttgccc
    14161 tgaccgtggt gatttttatg gcgaagaaaa tatggcttga gcgccagaag aggaatgact
    14221 gatgggttta ccgccgctta gcaaaattcc tttcatttta cgtccgcagg cgtggctgca
    14281 tcgtcgccat tacggcgagg tgctaagccc gattcgctgg tgggggcgga tcccgtttat
    14341 cttttatttg gtgtcaatgt ttgttgggtg gctggagcgc aaacgctcac cgctcgatcc
    14401 ggtagtgcga tcgcttgtca gcgcgcgcat tgcgcaaatg tgcctgtgtg agttttgcgt
    14461 ggatatcacc agtatgaaag tcgccgagcg taccggcagc accgataaac tgctggcagt
    14521 ggctgactgg cggcaaagcc cgctctttag cgatgaagaa cggctggcgc tggagtacgc
    14581 cgaagccgca agcgtcacac cgccaacagt cgatgatgcc ctgcgtaccc gactggctgc
    14641 gcattttgac gctcaggcgc tcaccgaact gacggcattg atcggcctgc aaaacctgtc
    14701 ggctcgtttt aattctgcca tggacattcc cgctcagggg ctgtgccgta ttccggaaaa
    14761 acgttcttaa ggagggaaga tgcgccattg tgggtggttg ctgggattgt tatcgctgtt
    14821 ttctttggca acacatgcca gtgactggca ggaaattaaa aatgaggcca aagggcaaac
    14881 cgtctggttt aacgcctggg gcggcgatac cgcaattaac cgctatctcg actgggttag
    14941 cggcgagatg aaaacccatt acgctataaa cctgaagatt gttcgtctcg cggatgccgc
    15001 agacgcggtg aagcgcattc agaccgaagc tgctgccggt cgtaaaacgg gcggctcggt
    15061 ggatctgctc tgggtgaacg gcgaaaactt ccgcacctta aaagagtaat gccagtcagt
    15121 taagcaactg actggcatt
//
LOCUS       DAZSNO010000089        15076 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZSNO010000089 DAZSNO010000000
VERSION     DAZSNO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15076
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.089"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(40..2319)
                     /gene="maeB"
                     /locus_tag="ACHWN2_002635"
     CDS             complement(40..2319)
                     /gene="maeB"
                     /locus_tag="ACHWN2_002635"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR8932115.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2608..3558
                     /gene="tal"
                     /locus_tag="ACHWN2_002636"
     CDS             2608..3558
                     /gene="tal"
                     /locus_tag="ACHWN2_002636"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR8932116.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3578..5581
                     /gene="tkt"
                     /locus_tag="ACHWN2_002637"
     CDS             3578..5581
                     /gene="tkt"
                     /locus_tag="ACHWN2_002637"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR8932117.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5684..6727)
                     /locus_tag="ACHWN2_002638"
     CDS             complement(5684..6727)
                     /locus_tag="ACHWN2_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR8932118.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(6853..7428)
                     /gene="nudK"
                     /locus_tag="ACHWN2_002639"
     CDS             complement(6853..7428)
                     /gene="nudK"
                     /locus_tag="ACHWN2_002639"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR8932119.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7496..9475)
                     /gene="aegA"
                     /locus_tag="ACHWN2_002640"
     CDS             complement(7496..9475)
                     /gene="aegA"
                     /locus_tag="ACHWN2_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR8932120.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9681..11398
                     /gene="narQ"
                     /locus_tag="ACHWN2_002641"
                     /pseudo
     CDS             9681..11398
                     /gene="narQ"
                     /locus_tag="ACHWN2_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11600..14713
                     /gene="acrD"
                     /locus_tag="ACHWN2_002642"
     CDS             11600..14713
                     /gene="acrD"
                     /locus_tag="ACHWN2_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR8932121.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14812..14871)
                     /gene="ypfM"
                     /locus_tag="ACHWN2_002643"
     CDS             complement(14812..14871)
                     /gene="ypfM"
                     /locus_tag="ACHWN2_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR8932122.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3539 a         4001 c         3935 g         3601 t
ORIGIN      
        1 ccctgaatat tcagggtaag cgtgagagtt aagaatgaat tacagcggtt gggtttgcgc
       61 ttcaaccacg gccagcgcca ccatattgac gatacgacgc accgatgcga tcggcgttaa
      121 cacgtgaacc ggtttcgcca cgcccatcag caccggaccg acagtcacac cttccgagct
      181 ggaaacgcgc agtaagttgt aactaatgcg ggcagcttcc atgttcggca tcaccagaat
      241 attggcagaa cctttcaaag ggctgtccgg catacggtcg ttgcgaatcg cttccaccag
      301 cgctgcatcg ccgtgcattt caccatcaat catcagatct ggcgcacgat ccctgaccag
      361 ttccagcgcc tgacgcattt tgctcgacga cgggcagtca gaagaaccaa agttggagtg
      421 cgacaacaga gcaacgcgcg gctcaatacc aaaacgacgg acagtttctg ccgccatcaa
      481 ggtgatctcc gccagctctt ctgcgtctgg ttcgtcattg acgtaggtat cggcaataaa
      541 ggtgttacca ctcggcagca gcagcgcatt cattgcacct gcggtgtgaa cgccatcgcg
      601 ataaccaaag acatttttca ccacactaaa atgttcatga taatcgccca ccgtaccgca
      661 gatcattgca tcggcttccc cacgctgaac catgatcgcg ccgatcactg tcgggttact
      721 gatcagcgca cgctgcgcct gttcctgagt gacaccgcga cgcttcatga tctggaagta
      781 ttcggtccag tactctttaa agcgcggatc ggattcgtta ttgacgattt caaaatcaac
      841 gcccgctttg atctgcaagc ccagtttctg aatgcgcatt tcgatcacgt tcggacgacc
      901 gataaggatc ggtttcgcca gtcccagcgt taccagttcc tgagtcgcat gcagaacgcg
      961 cgcctcttcc ccttccggca gaactacgcg cttcggcgct ttgcgagcct gggagaaaat
     1021 cggcttcata aacaggttgg ttttgtaaac gaactcagtc agcttgtcga tgtagacgtc
     1081 gaaatcagca atcggacgcg tcgccacgcc cgactccatc gccgcttttg cgaccgcagg
     1141 agcgatctta acgatcaagc gcggatcaaa cggttttgga atgatgtatt ccggaccaaa
     1201 gctcagatcc tgaacgccat acgctgaagc caccacttca ctctgttctg catgggccag
     1261 ttctgcaatc gcgcgtaccg ccgccagttt catctcttcg ttgatggcgg ttgcgccaac
     1321 gtccagcgcg ccacggaaga tgaacgggaa gcaaagaacg ttgttcacct ggttcgggta
     1381 gtcggaacgg ccggtacaga tgatggcatc cggacgcact tctttcgcca gcggcggcag
     1441 aatttccggt tccgggttcg ccagcgccag gatcattggt gcccgagcca ttttcttcac
     1501 catttcctgg gtcagcactt tcgggccgga acagcccagg aaaatatccg caccttcaat
     1561 cacatcatcg agggtacgtt tgccgtcatc caccaccgca tatgcggctt tggtttccgc
     1621 catatttggc tcacggccct gatagataac gccttttgaa tcgcaaacca cgatgttatg
     1681 tttttgcagc cccagcgcta ccagcaggtt catacaggcg attgctgcgg cacccgcgcc
     1741 ggaaaccacc atccgcacgt cggagatgtt tttctccacc acgcgcaagc cgttgaggat
     1801 ggcggcagtg ctgatgattg ccgtgccgtg ctgatcgtcg tggaataccg gaatattcat
     1861 ccgctcgcgc agtttctgtt caatatagaa acattctggc gctttaatgt cttcgaggtt
     1921 gatgccgccg aaggtcggtt cgagcgcggc gacgacttca ataaatttgt ccgggtcgag
     1981 ttcatcaact tcaatgtcaa atacatcaat cccagcgaat ttcttaaata gaacgccctt
     2041 gccttccatc accggtttgc ctgccagcgc gccaatgttg cctaacccca gcaccgccgt
     2101 accgttagag atcaccgcaa ccaggttacc gcgcgcggta tatttgtagg cttttagcgg
     2161 gtctttttcg atttcaagac aaggtgcggc aacgcctggt gagtaggcca gcgccagatc
     2221 gcgctgcgtt gccagaggct tggttggaga aacctggatt ttccctggaa ctggaaattc
     2281 atggaaatca agtgcacttt gttttaactg gtcatccatt tggttgttcc tttcacgtaa
     2341 cgttcacaaa taaagtgtgt gggcaacagc ccctgcccac aacgtggcgc acattattac
     2401 cctgccggag tctacagact ttgagcaagt ccaaactctc accattaata taatgttttg
     2461 gtaataatcc tataacactg atgttacctg cttaatccag caataccatg cctgtctgct
     2521 atgctttttt gatgcgttta gcgaaatttc tcagaagtgt gaattaacgc actcatctaa
     2581 cactttactt ttcaaggagt atttcctatg aacgagttag acggcatcaa acagttcacc
     2641 actgtcgtgg cagacagctg cgatattgag tccattcgcc attatcatcc ccaggatgcc
     2701 accactaatc cttcgctgtt actcaaggct gccggattat cacaatatga gcatttaata
     2761 gacgatgcta tcgcctgggg taaaaaaaat ggcaagaccc aggaacaaca ggtggtcgca
     2821 gcgtgtgaca aactggcggt caatttcggt gctgaaattc tcaaaatcgt acccggttgc
     2881 gtgtcaacag aagttgatgc acgcctctct tttgataaag aaaagagtat tgagaaggcg
     2941 cgccatctgg tggacttgta tcagcaacaa ggcgttgaga aatcacgcat tttgatcaaa
     3001 ctggcttcga cctgggaagg aattcgcgcg gccgaagagc tggaaaaaga aggtattaac
     3061 tgcaacctga cgctgctgtt ttcttttacc caggcgcggg cctgtgcgga agcaggcgtt
     3121 tttctgattt cgccgtttgt agggcgtatt tatgactggt atcaggcacg caagccgatg
     3181 gacccgtatg tggtggaaga agatccgggc gttaaatcgg tgcgcaatat ctacgactac
     3241 tataagcaac accactatga aaccattgtg atgggcgcga gcttccgtcg caccgaacaa
     3301 atcctcgcct taaccggctg cgatcgactg actatcgcac cgaatttgct gaaggagctg
     3361 caggaaaaag tttctccagt ggtacgtaaa ttaatcccgc cttctcagac gttcccacgc
     3421 ccagcaccca tgagcgaagc ggagttccgt tgggagcaca atcaggatgc gatggcggta
     3481 gaaaaactgt ctgaaggcat tcgtctgttc gctgttgatc aacgcaaact ggaagatctt
     3541 cttgccgcca aactataaac cagccacgga gtgttatatg tcccgaaaag accttgccaa
     3601 tgcgattcgc gcactcagta tggatgctgt acaaaaagcc aattctggtc atcccggcgc
     3661 gccgatgggc atggctgata ttgccgaagt gctgtggaac gattttctta aacataatcc
     3721 taccgaccca acctggtatg atcgcgaccg ctttattctt tccaacggtc acgcgtcgat
     3781 gctgctctac agtttgctac atctgaccgg ttacgacctg ccgctggaag aactgaagaa
     3841 cttccgtcag ttgcattcga aaaccccagg ccacccggag attggctata cgccaggcgt
     3901 tgaaaccacc accggcccgc ttggacaagg tttggcgaac gccgtcgggc tggcgatagc
     3961 agagcgtaca ctggcggcgc agtttaacca gccagaccat gagatcgtcg atcacttcac
     4021 ctatgtgttt atgggcgacg gctgcctgat ggaaggtatt tcccacgaag tctgttcgct
     4081 ggcaggcacg ctgggactag gcaagctgat tggtttttac gatcacaacg gtatttccat
     4141 cgacggtgaa acagaaggct ggtttaccga cgatacggca aaacgttttg aagcctatca
     4201 ctggcatgtg atccatgaaa tcgacggtca cgatccgcag gcggtgaagg aagcgatcct
     4261 tgaagcgcaa agcgtgaaag ataagccgtc gctgattatc tgccgtacgg tgattggctt
     4321 tggttcgccg aataaagcag gtaaggaaga ggcgcacggc gcaccactgg gggaagaaga
     4381 agtggcgctg gcacggcaaa aactgggctg gcaccatccg ccatttgaga tccctaaaga
     4441 gatttatcac gcctgggatg cccgtgaaaa aggcgaaaaa gcgcagcaga gctggaatga
     4501 gaagtttgcc gcctataaaa aggctcatcc gcaactggca gaagagtttc cccgacggat
     4561 gagcggtggt ttaccgaagg actgggagaa aacgactcag aaatatatca atgagttaca
     4621 ggcaaatccg gcgaaaatcg ctacccgtaa ggcttcgcaa aatacgctta acgcttacgg
     4681 gccgatgctg cctgagttgc tcggcggttc ggcggatctg gctcccagca acctgaccat
     4741 ctggaaaggt tctgtttcgc tgaaggaaga tccggcgggc aactacattc actacggggt
     4801 gcgtgaattt ggcatgaccg ctatcgccaa cggcatcgcg caccacggcg gctttgtgcc
     4861 gtataccgcg acgttcctga tgtttgttga atacgcccgt aacgccgcgc ggatggcggc
     4921 actgatgaaa gcgcggcaga ttatggttta tacccacgac tcaattggcc tgggcgaaga
     4981 tggtccgacg caccaggctg ttgagcaact ggccagcctg cgcttaacgc caaatttcag
     5041 cacctggcga ccgtgcgatc agatggaagc ggcggtgggc tggaagctgg cggttgagcg
     5101 ccacaacgga ccgacggcac tgatcctctc aaggcagaat ctggcccagg tggaacgtac
     5161 gccggatcag gttaaagaga ttgctcgtgg cggttatgtg ctgaaagaca gcggcggtaa
     5221 gccagatatt attctgattg ccaccggttc agagatggaa attaccctgc aagcggcaga
     5281 gaaattagca ggagaaggtc gcaatgtacg cgtagtttcc ctgccctcga ccgatatttt
     5341 cgacgcccag gatgaggaat atcgggagtc ggtgttgcct tctaacgttg cggctcgcgt
     5401 ggcggtggaa gcaggtattg ccgattactg gtacaagtat gttggtctga aaggggcaat
     5461 tgtcgggatg acgggttacg gggaatctgc tccggcggat aagctgttcc cgttctttgg
     5521 ctttaccgcc gagaatattg tggcaaaagc gcataaggtg ctgggagtga aaggtgcctg
     5581 atggtgatcg ccggatgctg tatgttcccc tcaccctaac cctctcccca gaagggcgag
     5641 gggacggtcc gtgcgctttt tagggccgca tctgcgattt tgtttaacgg gtgatccaca
     5701 acgtgggcct ggcgtctggc ccatgccagt tatcgcaggt aggttctgcg gcgtaacgca
     5761 ccaggcgaaa acgctgaccg tcaaagcgcc agcgcgcctg aatgccacaa tcgcttaatc
     5821 cgcgcccttt cgctaaggtc accagttcac gcgatttctc atcaaatgtt gcgttcatca
     5881 gttccagttc attcgtctcc tggccgttgt tgaacggcaa acgcaaccga accgggcgcg
     5941 aggctagcgg ctttttacgc gacacaatcc atgccaaatc aatggtgtta taggcccctg
     6001 cctcacagct aatcatcatc agtgctttat catcggtcag cgcagtgaca ttcacctcac
     6061 gacgtaacgg atcaagcgag cagcgcagac cattcatccg ccagtttcca taatccagca
     6121 aatcgttacg ttcttcgagt gagagtggcg tcggcgttgg gttaaccacc gcgacctctt
     6181 tcagcgcagg cgcgggcggt acgctgagag gcggttcgtc ccctttcttg atccacgcgg
     6241 tttcactgcc aacgcgcttt tgctgagcat caataaacaa caacgccgct tttaagccgc
     6301 tcagagaaat ggtctgattg ccgtcgcgta aggtgatcgc cttcccttcc tgaattatct
     6361 gcaaaaacgc ggtgatggtt gccgtatcat cggttactaa taaccatggt gaaatccgcc
     6421 acttgtcacc acttaatgcc aacggctcac catctaatag cagccgtggc gctatctccc
     6481 cttcagacgc atccggcgac ttcaacccac cgcgctcaat acgtaaaacg gcatcggtat
     6541 gcgccccagc gctgcggctc agggtcatca ccagtccatt atgatcgccc gtattgcgcg
     6601 ccacacaaaa attttgatta ttacaggtga cctgccagtc ggaaaacgcc cgttgcgctg
     6661 gtgccgccca caccaaagac gtcggcaaca aagcgaaaaa aaagagaagg aaaatgcgat
     6721 agcgcatgga cggtacgacc ccagaagttc aaacaaacag cacaagtcgt atcttcgttg
     6781 cgcaggtagc gcggctcaat cggatttgtc ggattgagcc aataataaac aacgaaatat
     6841 tattgctatt tttcagtcca ttaaatgtga catttgcaaa tagttaagca ataacaccgt
     6901 cttaccgtca cgtatctcgc cggttttgat catctccagc gcctggctga acggcagctc
     6961 gagcacttca atatcttcat cttcaacacc gccaccggcg ttagcgcgct gattgtcact
     7021 gtattcggcg ataaaaaagt ggattagctc agtcacaccg ccaggcgaca tatacagttc
     7081 aaataatttg cgcacttcgc ccacttcata gcccgtctct tcaatcgctt ctttacgaat
     7141 gcacacttcc ggttcgtcgt tatccagcag cccggcgcag gtttcaatca gctgcccgct
     7201 ttcattgcca ttaacccagg tagcgacacg gaactgacga atcagaacca cgctcttttt
     7261 cttcgcgttg tacaggagga tcgtcgcacc attaccgcga tcgtaaactt cacgtttatg
     7321 gcggataact tcgccatctt tgcgcgtgag atcgtaagta atgttgtgca gggtgaaata
     7381 gttatcggag agaattttgt ctttaatgag ggtgatttgt tgcgtcatac cgactccaca
     7441 gcgcgaaatg aacaattata ttacgctgtg aagtcgggtt tgtctgcgca ggctatcagt
     7501 gagatttgac tgattttacc cccagccagt caataatccc ctgtgccgca tgacgtcctt
     7561 ctgccattgc ggtaaccacc agatccgcac cacgcacggc gtcgccaccg gcgaagattt
     7621 tcggattggt ggtctggtaa cggtactggc tttccacatc cgcgatgatg cggccccatt
     7681 tgtctaccgt tacaccgtgc gactccagcc acggcatccc gtgtggattg aagccaaacg
     7741 ccataatcac cgcatcggct ggcatgacaa attcactgcc ctcaatcggt acaggtcgcc
     7801 gacgcccctg ggcatccggc tcgccaagac gcgtgcgcag gaaacgaatt ccgcaaacgt
     7861 gaccttgctc attcagctca agcgccaccg gctggacgtt aaattcgaag ttggccccct
     7921 cttcgcgggc gttcttcacc tctttcttcg agcctggcat gttagcttca tcacgacgat
     7981 aagcgcaggt gacgttactc gcgccgtggc gcagcgcagt acgcacacag tccatcgcgg
     8041 tgtcgccgcc gcccagtacc acgacgttaa gtccggcggt attgataaac ggctcttccg
     8101 gtagctcttc gagccccatc acctgtttag tgttggcaat gaggaacggc agcgcgtcat
     8161 aaacgcccgg cgcatcttca ttgggtaaac ccgctttcat ggaacggtaa gtgcctacgc
     8221 caacgaagac cgcgtcgtat tgttccaaaa gcaaatccaa agagacatct ttacccactt
     8281 cacaattgag ttcgaagtga atccccatcg cgctgaagat ttcccgacgg cgtgccagca
     8341 gggatttatc cagtttgaaa gaaggaatgc cgaaagtgag caagccaccg atttctggat
     8401 ggcgatcgta caccgtcacc gccacgccat tgcgggtcag aacatccgca caggccagcc
     8461 ctgccggacc tgccccgata atcgccaccc gcttgtccac tttggtgaca tgacttaagt
     8521 caggacgcca acctttcgcc aacgcctgat ctgaaatata gcgttcaatg ttgccgatag
     8581 ttaccgcgcc gtgctcatcg cgaatagtac aggcaccttc acacaaacgg tcttgcggac
     8641 aaacgcgtcc ggtaatttcc ggcagggtgt tggtctggtg agaaagctcg acggcggcgt
     8701 cgatgtttcc ggctttcacc agttcaatcc actgcggaat atggttatgc agcgggcagg
     8761 tccattcaca aacgctgtgc tcgccgaact taaggcagcg cgaggcttcc cgttgtgcct
     8821 ggtcggcgcg aaatggcaga taaatttcat caaaaccggt tttgcgcgct tcaatcgcca
     8881 gtttatccgg ctcgccacgc gcgggcgttg cctgcatttg ttcgacttta ctcattaccg
     8941 gcatttcttg tgcagcggta ctggcatgcc acggttgatg ctcctgacgt gcggtgcgca
     9001 agcggcggga tttcgccatg ccggagagtg cgacgtcagt gaccagttgc agcgcgtccg
     9061 ccgggcaatt ctcgacacag gcaggaccgt tttcgcgccc tgcacaaagg tcgcatttat
     9121 gcgccgtggc ttttactttt cctgccgcga cgggtgtcag gacgatttgc atcgtaccaa
     9181 acggacaggc caccacgcag gatttacagc caatacactt ttgctgattg acctgaatgc
     9241 tgtcatcaac gtggctgatt gcgccattag ggcagctacg ggcgcagggc gcatcttcac
     9301 aatggtgaca ggtcactgca ctacgttgct gctgatgttt gataaccgta attcggggat
     9361 gaaaatggtg ttggctcagg acatgttgct catcattgtg agccatgaca caggcgattt
     9421 cacaagcatg acaacccaga cattgctgac tgttggccat aataaaacga ttcataacga
     9481 ccttcttttt tggttgtaaa aaccttattc tttatatgag tgttgttatt acccgactta
     9541 caggggaacc ggcaatgttc atgtgcccag aataagtaac tatttcgcta taaactgtgg
     9601 cagatcaaat aatcccatca ctgactaaat tgcgtttcag cgaactggaa cattaatgat
     9661 tttttgtgga gaagacgcgt gtgattgtta aacgacccgt ctcggccagt ctggcccggg
     9721 ccttttttta cattgtgctg ctgtcgattc tttccacggg tatcgctctg ctaactctgg
     9781 cgagcagttt gcgcgacgct gaggctatca atattgccgg atcgctgcgt atgcagagtt
     9841 accgcctggg ctacgacttg caaagtggca gtccacaact caatgcacat cgccagttgt
     9901 ttcagcaggc actgcattca ccggtattaa ccaacctcaa cgtctggtat gtgccagaag
     9961 cagtaaaaac tcgctatgcg catctgaatg ccaactggct ggagatgaat aatcggctca
    10021 gcaagggcga tttgccgtgg tatcaggcca atattaataa ttatgttaat cagatagacc
    10081 tgttcgtgct ggctttacag cactacgctg aacgcaaaat gctgctggtg gtggcgattt
    10141 ccctggctgg cggtatcggt attttcacgc tggtcttttt tactttgcgc cgcatacgcc
    10201 atcaggtggt tgccccgctg aatcagctgg ttaccgccag tcagcgtatt gaacacgggc
    10261 agttcgactc gccgccgctg gataccagtc tgccgaatga gcttggtctg cttgcaaaaa
    10321 cctttaacca aatgtcgagc gagctgcata aattgtacct ttcgctggag gcgtcagtag
    10381 aagaaaagac ccgcgatctc cacgagacca agcgtcgtct ggaggtgttg tatcagtgca
    10441 gtgttcccag gcgctgaaca ccagccagat tgatgtgcat tgtttccgcc atattttgca
    10501 gattgttcgc gacaatgaag cagctgaata tctggagctg aacgttgggg ataactggcg
    10561 gattagcgaa gggaagccaa accctgagct gccaatgcag attttaccgg tgacaatgca
    10621 agagacggtt tacggcgaac tgcactggca aaatagtcac gtttcatcat cagaaccgct
    10681 gcttaacagt gtttcatcga tgctgggacg cggtttgtac tttaatcagg cgcagaagca
    10741 ttttcagcag ttattgttga tggaagaacg tgcgaccatc gcccgcgaat tgcacgactc
    10801 gctggctcag gtgctttctt acttacgtat ccagttgacg ttactgaagc gttcgatacc
    10861 ggaagataac gccaccgcac aaagtatcat ggccgatttt tcccaggcgt tgaatgatgc
    10921 ttatcggcag ttacgcgagc tgttgactac tttccgcctg acgctgcagc aggcggatct
    10981 cccctccgcg ttgagggaaa tgctggatac gttacaaaat caaaccagcg ctaaactgac
    11041 cctcgactgc cgtctgccaa ccctggcact ggatgcgcaa atgcaggtgc atttgttgca
    11101 aattattcgc gaagcggtgc tgaatgcgat gaagcacgcc aacgccagcg aaatcgccgt
    11161 cagttgcgtc accgcgccgg acggcaatca cacggtttat atccgtgata acgggattgg
    11221 tatcggtgaa ccgaaagaac ccgaaggtca ttatggactg aatatcatgc gcgaacgcgc
    11281 ggaacggctg ggcgggacgc tgactttttc acaaccttcc ggcggcggca cgttagtgag
    11341 tattagcttt cgctccgcag agggtgaggg gaaggggaac acgccggctg aggtgtaaat
    11401 aattaccctc acaccggccc tctcccttta aaggcgagag ggaaaaacgt gcccatattg
    11461 acctgtaagc gagtgtgtca gcaatctcat gataatttac attaactcct ttttttctcc
    11521 acgattggct cgtaccttgc cgctacagtg aagcaagtca agcctacaac aatacgcaga
    11581 aacacgaggt cctcttttaa tggcgaattt ctttattgat cgccccattt ttgcctgggt
    11641 gctgacaatc ctgttgtgtc tgacaggtac cctggcgatt ttttcattgc ccgttgaaca
    11701 ataccccgat ctcgcgccac cgaatgtgcg agtgaccgct aactatcccg gcgcatcggc
    11761 ccagacgctg gaaaacaccg tgacccaggt tatcgagcaa aatatgaccg gcctcgataa
    11821 tctgatgtat atgtcatctc agagcagtgg caccggtcag gcatctgtca ctttaagttt
    11881 taaagcaggc accgatccgg acgaagccgt gcagcaagta caaaaccagc tgcaatcagc
    11941 catgcgaaag ttaccgcagg cggtgcaaaa tcagggcgtg acggtgcgta aaaccggcga
    12001 taccaacatt ctgaccattg ccttcgtctc taccgatggc tcgatggata aacaggatat
    12061 tgctgattat gttgccagta atattcagga cccgttaagc cgcgtgaatg gcgtcgggga
    12121 tatcgatgcc tatggttcgc aatattccat gcgtatctgg ctggacccgg cgaaactcaa
    12181 cagtttccag atgacggcta aagatgtcac tgatgccatt gagtcacaga acgcgcagat
    12241 tgcggttggg caacttggtg gtacaccttc cgtcgataag caggcgctca acgccaccat
    12301 taacgcccag tcactgctgc aaacaccaga acagttccgc gatatcacct tgcgggtgaa
    12361 tcaggacggc tcagaggtaa ggctgggcga tgtcgccacc gtcgaaatgg gggcggagaa
    12421 atacgattat cttagccgct tcaatggtaa gccagcctcc gggttggggg taaaactggc
    12481 ctccagcgca aacgaaatgg cgacagcgga gctggtgctc aatcgtctcg acgagctggc
    12541 gcagtatttc ccgcacggac tggaatacaa ggtggcgtat gaaaccacct cgtttgttaa
    12601 agcctccatt gaagacgtgg tgaaaacgct gctggaagct atcgctctgg ttttcctcgt
    12661 tatgtatctg ttcctgcaaa acttccgcgc cacgctgata cccactatcg ccgtgccggt
    12721 ggtgttgatg ggaaccttct ccgtacttta cgccttcggt tacagcgtca acaccttaac
    12781 catgttcgcg atggtgctgg cgatcggtct gctggtggat gacgccatcg tggtggtgga
    12841 aaacgtcgaa cgtattatga gtgaggaagg actcactcct cgcgaagcca cacgtaaatc
    12901 gatggggcag atccaggggg cactggtcgg gattgcgatg gtgctttcgg cggtatttgt
    12961 accaatggcc ttcttcggcg gcaccaccgg tgccatctat cgccagttct ctattaccat
    13021 tgttgcggcg atggtgctgt cagtactggt agcgatgctc ctcactccgg ctctgtgtgc
    13081 cacgctactt aagccactga aaaaaggtga gcatcacggg caaaaaggtt tttttgcctg
    13141 gtttaaccag atgtttaacc gcaacgccga acgctacgaa aaaggggtgg cgaaaattct
    13201 ccaccgtagc ctgcgctgga ttgtgattta tgtcctgctg cttggcggca tggtgttcct
    13261 gttcctgcgt ttgccgacgt cgttcttacc gctggaagac cggggcatgt ttactacctc
    13321 ggtacaattg cccagcggtt caacccaaca acagaccctg aaagtcgttg agcaaatcga
    13381 gaaatattac ttcacccatg aaaaagacaa catcatgtcg gtgtttgcca ccgttggttc
    13441 tggccctggg ggtaacgggc aaaacgtggc gagaatgttt atccgcctga aagactggag
    13501 cgaacgcgac agtaagaccg gcacctcgtt tgccattatc gagcgtgcaa cgaaggcgtt
    13561 taaccaaatt aaagaagctc gcgttatcgc cagcagccca ccagcaatta gcggtcttgg
    13621 tagttctgca ggttttgata tggagttgca ggaccacgct ggagcgggtc acgatgcgct
    13681 gatggcagca cgtaatcagt tgctggcgct ggcggcggaa aacccggagc taacccgtgt
    13741 gcgccataac ggcctcgatg acagtccgca gttgcagatt gatatcgacc agcgtaaagc
    13801 tcaggcgctg ggcgttgata tcgacgatat taacgacaca ctgcaaaccg cctggggttc
    13861 gagctatgtg aatgacttta tggatcgcgg tcgcgtgaag aaagtctatg tgcaggcagc
    13921 tgcgccgtat cgcatgctgc cagatgacat caatctctgg tatgtccgaa ataaagatgg
    13981 cggcatggtg cccttctctg ctttcgcgac ctcacgctgg gaaacaggct cgccgcgtct
    14041 ggaacgctat aacggttatt ctgcggttga gattgttggg gaagccgcac cgggggtcag
    14101 taccggtacg gcgatggata ttatggaatc gttagtgaag cagctgccaa acggctttgg
    14161 tctggagtgg acggcgatgt cgtatcagga gcggctttcc ggcgcgcagg ctccggcgct
    14221 gtacgccatt tccttgctgg tggtattcct gtgtctggcg gcattgtatg aaagctggtc
    14281 ggtaccgttc tcggtaatgc tggtcgtgcc gctgggggta atcggcgcgc tgctggcaac
    14341 ctggatgcgc gggctggaaa acgacgttta cttccaggtg ggcctgttaa cggtcattgg
    14401 tttatcggcg aaaaacgcca tcctgatcgt cgagtttgct aacgagatga accaaaaagg
    14461 ccatgacctg tttgaagcga cgctccacgc ctgccgtcag cgtttacgcc cgattctgat
    14521 gacctcgctg gcatttatct tcggcgtatt gccaatggca accagcacgg gtgccggttc
    14581 cggtggtcag catgcggtgg gtactggcgt aatgggcggg atgatttcag ctactattct
    14641 ggctatttac ttcgtgccgc tgttctttgt gctggtgcgc cgccgcttcc cgctgaagcc
    14701 gcgcccggaa taagcaataa aaaaggcgac atgccaatgt gtcgcctttt tcaactttcc
    14761 gattaagaac ctgctcagcg ggttcttgct gtttatactt tgtctcagga attacttacg
    14821 aagcataact tcgataaagt ctttccagtt ccccagttca cgttcaatca taacaacctc
    14881 tcttataatt atgggtattc tacggaaaca atataccgtg gtgaagctaa tttactcgat
    14941 tgctgcgatg actacctccg ggggacaaac cttatgtaaa tactatggtc ctgcggtgat
    15001 aatttgtatg tgatacacag caacatttcg agatattcat acggcatcta atactgattt
    15061 aattctggtt aaaata
//
LOCUS       DAZSNO010000090        14901 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZSNO010000090 DAZSNO010000000
VERSION     DAZSNO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14901
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.090"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            98..340
                     /gene="yjbE"
                     /locus_tag="ACHWN2_002644"
     CDS             98..340
                     /gene="yjbE"
                     /locus_tag="ACHWN2_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR8932123.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            454..1092
                     /locus_tag="ACHWN2_002645"
     CDS             454..1092
                     /locus_tag="ACHWN2_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR8932124.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1089..1826
                     /locus_tag="ACHWN2_002646"
     CDS             1089..1826
                     /locus_tag="ACHWN2_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR8932125.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1826..3922
                     /locus_tag="ACHWN2_002647"
     CDS             1826..3922
                     /locus_tag="ACHWN2_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR8932126.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3969..4247)
                     /locus_tag="ACHWN2_002648"
     CDS             complement(3969..4247)
                     /locus_tag="ACHWN2_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932127.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4248..4391
                     /locus_tag="ACHWN2_002649"
     CDS             4248..4391
                     /locus_tag="ACHWN2_002649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932128.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4517..4927
                     /gene="psiE"
                     /locus_tag="ACHWN2_002650"
     CDS             4517..4927
                     /gene="psiE"
                     /locus_tag="ACHWN2_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR8932129.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4971..6446)
                     /gene="xylE"
                     /locus_tag="ACHWN2_002651"
     CDS             complement(4971..6446)
                     /gene="xylE"
                     /locus_tag="ACHWN2_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR8932130.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6817..7707)
                     /gene="malG"
                     /locus_tag="ACHWN2_002652"
     CDS             complement(6817..7707)
                     /gene="malG"
                     /locus_tag="ACHWN2_002652"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR8932131.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7722..9266)
                     /gene="malF"
                     /locus_tag="ACHWN2_002653"
     CDS             complement(7722..9266)
                     /gene="malF"
                     /locus_tag="ACHWN2_002653"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR8932132.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9420..10610)
                     /gene="malE"
                     /locus_tag="ACHWN2_002654"
     CDS             complement(9420..10610)
                     /gene="malE"
                     /locus_tag="ACHWN2_002654"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR8932133.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10975..12090
                     /gene="malK"
                     /locus_tag="ACHWN2_002655"
     CDS             10975..12090
                     /gene="malK"
                     /locus_tag="ACHWN2_002655"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR8932134.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12162..13502
                     /gene="lamB"
                     /locus_tag="ACHWN2_002656"
     CDS             12162..13502
                     /gene="lamB"
                     /locus_tag="ACHWN2_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR8932135.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13654..14574
                     /gene="malM"
                     /locus_tag="ACHWN2_002657"
     CDS             13654..14574
                     /gene="malM"
                     /locus_tag="ACHWN2_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR8932136.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14803..>14901
                     /locus_tag="ACHWN2_002658"
     CDS             14803..>14901
                     /locus_tag="ACHWN2_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932137.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRN"
BASE COUNT         3696 a         3831 c         3908 g         3466 t
ORIGIN      
        1 ccccgcgccg taattcgcat gctttagttg tgtatactcg atcccgcccg aaatgttttt
       61 gggtaaatct ccattcattc aatgaaggga aattgttatg aaaaaagttc tgtatggcat
      121 ttttgccata tctgcgcttg cggcgacttc tgcgtgggct gcacctgtac aggtgggcga
      181 agcggcagag tcggcagcaa cgtcggtttc ggcggggagt tcctccgcga ccagcgtcag
      241 caccgtaagc tcggcggtgg gtgtcgcgct cgcggcaacc ggtggcggtg atggttctaa
      301 taccgggacc accacaacca cgaccaccag tacccagtaa taaagtatgt atccccaaaa
      361 taatttgagt cattgcatct gtggcttgaa gtatgaaggg atttaccata accacactcc
      421 ggtgtggtta ttctgcccct ctggagaaga gtcgtgaagc gacctgcact cattcttatc
      481 tgcctgctat tacaggcctg ttcagccacg actaaagagc tgggcaattc actgtgggac
      541 agtctgttcg gcacgccagg cgtacagctg acggacgatg atattcaaaa tatgccctac
      601 gccagccagt acatgcagct taatggcggg ccgcaattgt ttgtggtgct ggccttcgct
      661 gaagacggac aacaaaaatg ggtcactcag gatcaagcca ctcttgtcac acaacatggt
      721 cgtctggtga agactttgct tggcggcgac aacctgattg aagtgaataa ccttgccgcc
      781 gacccgctga ttaaacccgc acaaattgtt gatggtgcaa catggacgcg cacgatgggc
      841 tggaccgagt accagcaggt acgctacgcc accgcacgct cagtcttcaa atgggatggc
      901 accgataccg tcaaggtcgg cagcgatgaa accctggttc gcgtgctgga cgaagaagtc
      961 tccaccgacc aggcgcgctg gcataaccgc tactggatcg acagcgaagg gcaaattcgc
     1021 cagtcggaac agtatctcgg cgcggattat ttcccggtga aaaccactct catcaaggcg
     1081 gcaaaacaat gattaaacaa actattgtcg cgttgctttt gagtgtggga gcgtcatcgg
     1141 tcttcgcggc aggaaccgtc aaggtgttca gcaatggcag cagtgaggcc aaaacgctaa
     1201 cgggcgcaga gcatttaatc gatctggtag ggcaacctcg actggcaaac agctggtggc
     1261 ctggtgcggt gattagcgaa gagctggcaa cggcggcagc attgcgtcag cagcaggcgt
     1321 tgctgacacg gctggcagaa cagggcgcag attccagcac cgacgatgcc gccgccatta
     1381 acgccttacg ccagcaaatc caggcattga aagtgacagg caggcaaaaa atcaatcttg
     1441 atcccgatat cgtccgcgtt gccgaacgcg gtaacccgcc gttgcagggc aactacacgc
     1501 tgtgggtcgg accaccgccg tccacggtca cgttgttcgg gcttatcagc catcctggca
     1561 atcagccatt cactccaggt cgcgacgtgg cgagctatct ctctgaccag agcctgctca
     1621 gcggtgcgga tcgcagctac gcgtgggtcg tttacccgga cggacgcacg caaaaagcgc
     1681 cggtggctta ctggaacaag cgtcacgtag agccgatgcc cggcagcatt atttatgttg
     1741 gcctcgcaga ttccgtctgg agtgagacgc ctgatgccct taacgccgac attcttcaga
     1801 ctctgacgca gcggataccc caataatgaa aaaaagacat ctgcttagct tactggcgct
     1861 gggcattagc acagcttgct acggcgaaac atatcctgcg cccattggtc cgtcgcagtc
     1921 ggatttcggt ggcgtaggat tattacaaac tcccaccgcg cgtatggcac gggaagggga
     1981 gttgagtctg aactatcgcg ataacgatca gtaccgttat tactcagctt cagtgcaact
     2041 cttcccgtgg ctggaaacaa cgctgcgcta caccgacgtg cgcacccggc agtacagcag
     2101 cgtcgaagcg ttctctggcg atcaaacgta taaagataaa gccttcgatc tcaaactgcg
     2161 tttgtgggaa gagagttact ggctgccgca agtggcggtt ggtgcgcggg atatcggcgg
     2221 tacggggctg tttgatgcgg aatatcttgt tgccagcaaa gcctgggggc cgttcgattt
     2281 tacgctcggc ctgggctggg gatatctggg caccagcggt aatgtaaaaa atccgctctg
     2341 ttcagccagt gataaatatt gctatcgcga taacagctac aaacaggcgg gatctatcga
     2401 cggtagccag atgttccacg gtcctgcctc actgtttggc ggcgtggaat accagacgcc
     2461 ctggcaaccg ctgcgtctga aactggagta tgaaggtaat aattatcagc aggattttgc
     2521 cgggaagctg gagcaaaaaa gtaagtttaa cgtcggggcg atttatcgcg ttaccgattg
     2581 ggccgacgtt aaccttagct atgaacgtgg caacaccttt atgtttggcg tcacgctgcg
     2641 caccaacttt aacgatctgc gcccgtccta catcgataac gcccgcccgc aatatcaacc
     2701 gcagccgcag gatgccattt tgcagcattc ggtggtggcg aatcagttaa cgttgctgaa
     2761 atacaacgcc ggacttgccg atccgcagat ccaggcgaaa ggcgatacgc tgtatgttac
     2821 cggcgagcag gtgaaatatc gtgattcgcg cgaagggatc atccgtgcta atcggatcgt
     2881 gatgaacgat ctgccggatg ggatcaaaac gatccgcatt acggaaaatc gccttaacat
     2941 gccgcaagtg acgacggaaa ccgatgtcgc cagcctgaaa aatcatctcg ccggagagcc
     3001 gttgggccac gaaacgacgc tggcgcacaa acgcgtcgag ccagtggttc cgaagtccac
     3061 cgagcagggc tggtatatcg acaaatcacg ctttgatttc catatcgatc cggtgctgaa
     3121 ccagtcggtc ggtggcccgg aaaactttta catgtatcag ctgggcgtga tgggaacggc
     3181 agatttgtgg ctgacggacc atctactgac caccggcagc ctgtttgcaa atcttgccaa
     3241 caactacgac aagtttaact acaccaatcc accgcaggac tcgcacttac cgcgcgttcg
     3301 tacccatgtg cgcgagtatg tgcagaacga tgtctatgtg aataacctgc aagccaacta
     3361 cttccagcat ctgggcaacg gcttctacgg tcaggtctat ggaggttatc tcgaaaccat
     3421 gtttggcggt gcgggggccg aagtgttgta tcgtccgctg gacagtaact gggcgtttgg
     3481 tctggatgcc aactacgtta aacagcgcga ctggcgtagt gcaaaagata tgatgaaatt
     3541 caccgactac agcgtgaaaa ccggacatct gaccgcctac tggacgccat ctttcgccca
     3601 ggacgtgttg gtgaaagcca gcgtcgggca gtatctggca ggggataaag gcggcacgct
     3661 ggagatcgct aaacgctttg acagcggcgt ggtggtgggt ggctatgcca cgatcaccaa
     3721 cgtttcgaaa gaggagtacg gcgaagggga cttcaccaaa ggcgtgtatg tctcggtacc
     3781 gttggatctc ttctcgtctg gcccgacacg cagccgtgcg gcgattggct ggacgccgct
     3841 gacgcgtgac ggtggtcagc aacttgggcg taagttccag ttgtatgaca tgaccagcga
     3901 ccgtagcgta aatttccgct aagtcatggg aaaggtgcca gttttcgcac tcactggcac
     3961 cctcattctt aatgacctgt tgagccatca ggaacgcagt tcacccccgg tttacgatct
     4021 ggtgtgtaaa ggcgaaaaca ttctgtcttc ctgtccgtcg atatcggttg caggtctggt
     4081 tgcctgagcg actgggccat cattgggctg gagacattgc tttgcggtag gtcggcttta
     4141 tcagcagtac cgagcggacc agcataagca ggaagaacag agactgataa catcaaagca
     4201 gcaaaataag gcttcatttt taccaccttt atcaggttac gtttcatttg ttccagagga
     4261 acattgtcga tttttcgcgc attgctggtg gctgggaatc acctgaatgg gtgatttttg
     4321 aattaccggc tttggtgcgg tttgtcttgc cggatgcgcc gccaggcgcg gcttatccgg
     4381 cctacgggta ggtatatccg gctttggtgg aggcgcgctc caaatccagg ttgaacaaaa
     4441 catacacaaa aaatatagat ctccgtcaca tttttgcgtt atacaggaag ctcgccactg
     4501 tgaaggaggt actgctatga cgtcactctc tcgtccgcgc gtggagttta tctccaccat
     4561 tttgcagacc gtactcaatc ttggcctgct gtgcctcggc ctgattttgg ttgtcttcct
     4621 cggcaaagaa acggtgcatc tggctgatgt gctgttcgcg ccagaacaaa ccagcaaata
     4681 tgagctggta gaaggactgg tggtttactt tctctatttc gaatttatcg cgctgattgt
     4741 gaagtacttt cagtccggtt ttcacttccc gctacgttac tttgtctata ttgggatcac
     4801 cgccattgtg cgcttgatca tcgtcgatca taaatcgcca ctggatgtgt tgatctactc
     4861 ggctgcgatc ctgttactgg tgatcaccct gtggctgtgt aattcgaaac ggctgaagcg
     4921 ggagtaaaaa gacagcacgc cgaaatggcg cggcgtgctg gacaggaaga ttacagcgta
     4981 gcagtttgtt gtgttttctt cgtttccggt tcccagagcg cttccagctc ctcaagggtt
     5041 ttacctttgg tttccgggac aaatttccac ataaacagtg ctgccagaac gcccatacaa
     5101 ccgtaaatcc agtaggagaa accgttgtgg aaatgggcca ccagccagga gtttttgtcc
     5161 atcatcggga aggtccagga gacgaagtag ttcgccagcc actgggccgc caccgcgatt
     5221 gccagcgctt taccacgaat agcattcggg aagatttccg acagcagtac ccagcatacc
     5281 ggaccccaag acatggcaaa ggcggcaaca tagaacagca tcgacagtag cgccacaata
     5341 cccgatgcct gagtgtaaaa cgcggtaccg aggctaaaca taccgattgc cattccgagt
     5401 gcgccgataa tttgcagtgg cttacgacca aatttatcca cagtcataat tgccagtacg
     5461 gtaaaggtga ggttgataac tccgacaata atggtctgca acagcgcgat atccgtgctg
     5521 gcccccagcg ttttgaacac ttccggcgcg tagtacagca ccacattgat gccgacaaat
     5581 tgctggaaga tggagagcat aacgccgatt acaatcacgc ccacgccaaa catcagcaga
     5641 cgaccaccgg ttttgcggcc atgatccagg gagtgtttaa tttcctgtac tgcctgagtt
     5701 gcaagcgtgt tgcccataat tttgcgcagg atactttccg tctgttcttg cttgccgcgt
     5761 gacatcagcc agcgaggact ttctggcacg gtatacagca gcattaagaa cagcagtgca
     5821 gggatacatt ccgaggcaaa catataacgc ctgccgtcag tattcagcca gctggcatca
     5881 ccggaacggg caataaaata gtttacgcag taaactaaaa gttgcccgaa aataatcgca
     5941 aactggttaa atgaaaccag ttttccgcga atatgagctg gagccagttc cgcaatatac
     6001 attggtgaaa gcattgaggc taaaccaacg ccaataccgc caataatacg ataaataaca
     6061 aattccggga cataacctgc cagataaata ggtactgtgt tgtccgggtt tatagaggta
     6121 aaaccaagtt ctggccaggc agaacctaca ccagaaataa aaaacaggac agcagaaatc
     6181 ttaagtgaat cacgacggcc gaagcggtta ctgcaataac caccgagggc accgccgatg
     6241 atgcaaccaa tcagagcgct ggctacgcaa aatcctaaca gggagttggc agcggattca
     6301 cttaagtttt gtggagcaac aaagacggta ttgagtgact caacagtacc ggaaataacg
     6361 gcggtatcgt agccaaataa taaaccacct aatgtagcga ctaaggtaat cgaaaatata
     6421 taactggaat tatactgggt attcattcag acctgcctta gaccattctg atcttttatc
     6481 agtgcattga tgttggaatt gtcaggcagt taaggcaatt atcatttttt acaatgcgtt
     6541 tacgtaatgt cttttctgtg atcttaattg tgataattat caaaaattga tgaaacaata
     6601 ctattgccgt gactcagagc acgaaagaga attatcgtaa gtgggaaaac aaataacgta
     6661 aaaataataa gttctatgat gggaatatta accggcgaac gattcagatt gcagacgaaa
     6721 gaaaaaaagg cgctccgtgg agcgccgaat aatagtcaca agttgggata acgtaagttg
     6781 agggtgcagc ggcataacat tggcagaaca acatctttaa cctttcacac cacctgccgt
     6841 caggccgttg accagccagc gttgagccag caagaagacg atggtgatcg gtaatgcaga
     6901 catcacggca gcggcggcaa agtcacccca caggtagttt tgcgggttga ggtattgctg
     6961 catccccacg gccagggtgt agctgtttac gtcacgcagt aacagcgacg cgaccggaac
     7021 ttcagtaatg gcagcgataa acgacaggat gaataccacc gccagaatcg gtactgacag
     7081 cggcaacagg acaaggcgga aggcctgcca cggtgtcgca ccatccagcg cagccgcttc
     7141 ttccagcgaa ctgtcgatgg tttcgaaata gcctttgatg gtccagacat gcagcgcaat
     7201 cccacccaga tacgcgaaaa ttacgccgcc gtgagtattc aggccaatga atggaatgta
     7261 ctcacccaga cgatcaaaca acgcatacaa cgcgaccagt gaaagtactg ccgggaacat
     7321 ctggaaaatc agcattcctt tcagcagcgt cgctttgcct ggaaagcgca tacgggcgaa
     7381 agcgtaggcg caggtggtgg agagcgccac aatgccaatc gcggaaatcc cagcgacctt
     7441 taccgagttc cacagccaca gcagtaccgg gaatggcggt ggcgtaatgc gaccatcagc
     7501 ctgttcaacg ctaaaaccta acgccagttt ccagtgatcc caggagattt gctccgggat
     7561 caggctaccg gtcgcgaagt ttccctgacg cagcgagata gcgacgacca tcagcagcgg
     7621 gaacataatc gccgcgataa aaagtagcag tagcaggtga gtaataaaca aacgtgcttt
     7681 ttgcgatttc ggttggacca ttgccatttt tgttatctcc cttaatcaaa cttcattcgc
     7741 gtggctttca ggttcactat cgccagcgca cccaccagca ggaagatcag cgtggcaatt
     7801 gctgccgcca gaccgaagtc ctgacccccg ccgccttcaa aagcgatacg gtaggtgtag
     7861 ttaacaagca ggtcggtata accggctggc gtggtcgtgc caagacgatc cgggccgccg
     7921 ttggttaaca gctgaatcag cacgaagttg ttaaagttaa aggcgaagct ggcgatcatc
     7981 agcggcgtca gcggtttaat cagcagcggc agcgtaatct taaagaagtt ctggaacgga
     8041 cctgcgccat ccattgctga ggcttcatac aaatcgtccg gaatcgcttt cagcaagccc
     8101 atgcagagga tcatcatgta cggataaccc agccaggtat tgacgataat cagcatcgtg
     8161 cgagcggtgg tcggatcgct gaaccaggcg ggcttcacgc caaacagcgc gctcaacatc
     8221 atgttgattt caccgaagct ctggttaaac aatcctttga aaatcaagat tgaaatgaac
     8281 gatggcaccg cgtagggcag aatcagcagg acgcgataga ccgctttgcc gcgcaacgct
     8341 tcccactgca ccagacatgc cagaaccatg ccgaccgcca ccgttaaaaa gacagtgatc
     8401 agcgagaaca ccacggtcca gacgaaaatg gcgaggaacg gtttctgaat gccttcgtcg
     8461 gtaaagacgc gggtaaagtt tttccagccg gtggtcacgg tgtaaccggg gcttagcttt
     8521 tcatcccccc agttgccgtc ggcggtaatg gactggtaaa agccaatttg gttattcgga
     8581 cgatatttca cgccactctg attattcgtc aacgtgccgt caccgtcgag tgtgtagagc
     8641 ggctgcgtgc cagaaaactg gcgcagggag ctcatcatca ctttgttgcc atccggcaga
     8701 atggcggtaa tgtcactcag cgcctgacga ttctgggtaa tcacgcgcag attcgcgcgt
     8761 tcgccttcgg gctgggcggt cgtttctttc agttgcagtt tttgctcgcc gccaaattta
     8821 aacgcgtcgg agaggtaatt tttgccggtt tcgccgtcgc tgagcgccag ttgccactca
     8881 tcgcccgccg ggtaaagacc aaagttatag gttttgcctg cttgccagga gcgatctaac
     8941 aacacttcct gcgcacgttc aaaagtcagc tggttagtgc tgctgtagtt ggtgaaggca
     9001 atggcgatgg tgcagaccag agggaagagg acgaataatc ccattccagc cattcccggg
     9061 taaacatagc gccaggcgta ggctttacga ttggcgaaaa tatacagccc cgctgaactc
     9121 aatatcagcg tggtaatggc gaacaggtat tccccttgtg cgtacattaa aacaacaagg
     9181 taacccacca gcaggccgag cagacctagc actgaccatt tcagcgcgtc gctttgccac
     9241 caatgtttct ttttaatgac atccatgggg ttcttcctca ttccaggacg gataaggctt
     9301 tcacgcctta tccgacaaca actgcctgat gcgacactga tgcgtcttat caggcctaca
     9361 tgcgtttcag ttttgtaggc cggacaaggc gttcacgccg catccggcat ttcacagcat
     9421 tacttggtga tacgagtctg cgcgtctttc agggcttcat cgacagtctg acgaccgctg
     9481 gcggcgttga tcaccgcagt acgtacggca taccagaaag cggacatctg cgggatgttc
     9541 ggcatgattt cacctttctg ggcgttttcc atggtggcgg caatacgtgg atctttcgcc
     9601 aactcttcct cgtaagactt cagcgctacg gcacccagcg gtttgtcttt attaaccgct
     9661 tccagacctt catcagtcag cagatagttt tcgaggaact ctttcgccag ctctttgttc
     9721 ggactggcgg cgttaatacc ggtgctcagc acgccaacga acggtttaga tggttgaccc
     9781 ttgaaggtcg gcagtaccgt tacaccataa ttcactttgc tggtgtcgat gttggaccat
     9841 gcccacgggc cgttgatggt catcgctgtt tcgcctttat taaaggcagc ttctgcgatg
     9901 gagtaatcgg tgtctgcatt catgtgtttg tttttaatca ggtcaaccag gaaggtcaga
     9961 cccgctttcg cgccagcgtt atccacgccc acgtctttaa tgtcgtactt gccgttttca
    10021 tacttgaacg cataaccccc gtcagcagca atcagcggcc aggtgaagta cggttcttgc
    10081 aggttgaaca tcagcgcgct cttacctttc gctttcagtt ctttatccag cgccgggatc
    10141 tcttcccagg tttttggcgg gttcggcagc aggtctttgt tataaatcag cgataacgct
    10201 tcaacagcga ttgggtaagc aatcagcttg ccgttgtaac gtacggcatc ccatgtaaac
    10261 ggatacagct tgtcctggaa cgctttgtcc ggggtgattt cagccaacag gccagattga
    10321 gcgtagccac caaagcggtc gtgcgcccag aagataatgt cagggccatc gccagttgcc
    10381 gcaacctgag ggaatttctc ttccagttta tccggatgct caacggtgac tttaattccg
    10441 gtatctttct cgaatttctt accgacttca gcgagaccgt tatagccttt atcgccgtta
    10501 atccagatta ccagtttacc ttcttcgatt ttggcgagag ccgaggcgga aaacatcatc
    10561 gtcgttaatg cggataatgc gaggatgcgt gcacctgttt ttattttcat aatctatggt
    10621 ccttgttggt gaagtgctcg tgaaaacacc taaacggact ctagtttctt tatacggcaa
    10681 cctctttcca tcctccttgc ccctacgccc caccgttgct ttgtgtgatc tctgttacag
    10741 aattggcggt aatgtgggga tgcgcacata aaatcgccac gatttttgca agcaacatca
    10801 cgaaattcct tacatgacct cggtttagtt cacagaagcc gcgttctcat cctcccgcct
    10861 cctcccccat aaaaaagcca gggggtggag gatttaagcc atctcctgat gacgcatagt
    10921 cagcccatca tgaatgttgc tgtcgatgac aggttgttac aaagggagaa gggcatggcg
    10981 agcgtacagc tgcaaaatgt aacgaaagcc tggggcgagg tcgtggtatc gaaagatatc
    11041 aatctcgata tccatgaagg tgaattcgtg gtgtttgtcg gaccgtctgg ctgcggtaaa
    11101 tcgactttac tgcgcatgat tgccgggctt gagacgatca ccagcggcga cctgttcatc
    11161 ggtgagaaac ggatgaatga cactccgcca gcagaacgcg gcgttggtat ggtgtttcag
    11221 tcttacgcgc tctatcccca cctgtcagta gcagaaaaca tgtcatttgg cctgaaactg
    11281 gctggcgcaa aaaaagaggt gattaaccaa cgcgtcaacc aggtggcgga agtgctgcaa
    11341 ctggcgcatt tgctggatcg caaaccgaaa gcgctctccg gtggtcagcg tcagcgcgtg
    11401 gcgattggcc gtacgctggt ggccgagcca agcgtatttt tgctcgatga accgctctcc
    11461 aacctcgatg ctgcactgcg tgtgcaaatg cgtatcgaaa tctcccgtct gcataaacgc
    11521 ctgggccgca caatgattta cgtcacccac gatcaggtcg aagcgatgac gctggccgac
    11581 aaaatcgtgg tgctggacgc cggtcgcgtg gcacaggttg ggaaaccgct ggaactgtac
    11641 cactatccgg cagaccgttt tgtcgccgga tttatcggtt cgccaaagat gaacttcctg
    11701 ccggtaaaag tgaccgccac cgcaatcgat caagtgcagg tggagctgcc gatgccaaat
    11761 cgtcaacaag tctggctgcc tgttgaaagc cgtgatgtcc aggttggagc caatatgtcg
    11821 ctgggtattc gcccggaaca tctactgccg agtgatatcg ctgacgtcat ccttgagggt
    11881 gaagttcagg tcgtcgagca actcggcaac gaaacccaaa tctatatcca gatcccttcc
    11941 attcgtcaaa acctggtgta ccgccagaac gacgtggtgt tggtagaaga aggtgccaca
    12001 ttcgctatcg gcctgccgcc agagcgttgc catctgttcc gtgaggatgg cactgcatgt
    12061 cgtcgactgc ataaggagcc gggcgtttaa gcaccccaca aaacacacaa agcctgtcac
    12121 aggtgatgtg aaaaaagaaa agcaatgact caggagatag aatgatgatt actctgcgca
    12181 aacttcctct ggcggttgcc gtcgcagcgg gcgtaatgtc tgctcaggca atggctgttg
    12241 atttccacgg ctatgcacgt tccggtattg gctggacagg tagcggcggt gaacaacagt
    12301 gtttccagac taccggtgct caaagtaaat accgtcttgg caacgaatgt gaaacttatg
    12361 ctgaattaaa attgggtcag gaagtgtgga aagagggcga taagagcttc tatttcgaca
    12421 ctaacgtggc ctattccgtc gcgcaacaga atgactggga agctaccgat ccggccttcc
    12481 gtgaagcaaa cgtgcaggga aaaaacctga tcgaatggct gccaggttcc accatctggg
    12541 caggtaagcg cttctaccaa cgtcatgacg ttcatatgat cgacttctac tactgggata
    12601 tttctggtcc tggtgccggt ctggaaaaca tcgatgttgg cttcggtaaa ctctctctgg
    12661 cagcaacccg ctcctctgaa gctggtggtt cttcctcttt tgccagcaac aatatttatg
    12721 actataccaa cgaaaccgcg aacgacgttt tcgatgtgcg tttagcgcag atggaaatca
    12781 acccgggcgg cacattagaa ctgggtgtcg actacggtcg tgccaacctg cgtgataact
    12841 atcgtctggt tgatggcgca tcgaaagacg gctggttatt cactgctgaa catactcaga
    12901 gtgtcctgaa gggctttaac aagtttgttg ttcagtacgc tactgactcg atgacctcgc
    12961 agggtaaagg tctgtcgcag ggttctggcg tcgcgtttga taacgaaaaa tttgcctaca
    13021 atatcaacaa caacggtcac atgctgcgta tcctcgacca cggtgcgatc tccatgggcg
    13081 acaactggga catgatgtac gtgggtatgt accaggatat caactgggat aacgacaacg
    13141 gcaccaagtg gtggaccgtt ggtattcgcc cgatgtacaa gtggacgcca atcatgagca
    13201 ccgtgatgga aatcggctac gacaacgtcg aatcccagcg caccggcgac aagaacaatc
    13261 agtacaaaat taccctcgca caacaatggc aggctggcga cagcatctgg tcacgcccgg
    13321 ctattcgtgt cttcgcaacc tacgccaagt gggatgagaa atggggttac gactacaacg
    13381 gcgatagcaa ggttaacccg aactacggca aagccgttcc tgctgatttc aacggcggca
    13441 gcttcggtcg tggcgacagc gacgagtgga ccttcggtgc ccagatggaa atctggtggt
    13501 aatagcaaaa cctgggccgg ataaggcgtt tacgccgcat ccggcataaa aacaggttgt
    13561 cattatctga aaggggcgaa agcccctctg attatcgggt ttagcgcgct attgcctggc
    13621 taccgctgag ctccagattt tgaggtgaaa acaatgaaaa tgaataaaag tctcatcgcc
    13681 ctctgtttat cagcagggtt actggcaagc gcgcctggaa ttagccttgc cgatgttaac
    13741 tacgtaccgc aaaacaccag cgacgcgcca gccattccat ctgccgcgct gcaacaactc
    13801 acctggacac cggtcgatca atctaaaacc cagaccaccc aactggcgac cggcggccaa
    13861 caactgaacg ttcccggcat cagtggtccg gttgctgcgt acagcgtccc ggcaaacatt
    13921 ggcgaactga ccctgacgct gaccagcgaa gtgaacaaac aaaccagcgt ttttgcgccg
    13981 aacgtgctga ttcttgatca gaacatgacc ccatcagcct tcttccccag cagttatttc
    14041 acctaccagg aactaggcgt gatgagtgca gatcggctgg aaggcgttat gcgcctgaca
    14101 ccggcgttgg ggcagcaaaa actttatgtt ctggtcttta ccacggaaaa agatctccag
    14161 cagacgaccc aactgctcga cccggctaaa gcctatgcca aaggcgtcgg taactcgatc
    14221 ccggatattc ccgatccggt tgctcgtcat accagcgatg gcttactgaa actgaaagtc
    14281 aaaacgaact ccagctccag cgtgttggta ggaccgctat ttggttcttc cgctccagct
    14341 ccggttacgg taggcaacac ggcggcacca gctgtggctg cacctgctcc ggtgccggtg
    14401 aagaaaagcg agccgatgct caacgacacg gaaagttatt ttaataccgc gatcaaaaac
    14461 gctgtcgcga aaggtgatgt tgataaggcg ttaaaactgc ttgatgaagc tgaacgcctg
    14521 ggatcgacat ctgcccgttc cacctttatc agcagtgtaa aaggcaaggg gtaattacgc
    14581 cccacagtgc tgattttgca acaactggtg cgtctcctgg cgcacctttt tttatgcttc
    14641 cttcctggga tatgagcgat tttttatagt acttcacttc ttcttcacta agaatgtcca
    14701 ttatttcaat gcattatcag agattctttt cctttcgccg gtagtgtctg gacgctcagg
    14761 ctacttttcc aggttatttt atttctgtat tacagaggtt ttatgatagg tcatatcaca
    14821 aattctggcg gcaataactc tttgatgaaa catgatgtgg tgcaaggaaa taatatagta
    14881 gatcttgatt tactacgtaa t
//
LOCUS       DAZSNO010000091        14877 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZSNO010000091 DAZSNO010000000
VERSION     DAZSNO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14877
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.091"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..831
                     /locus_tag="ACHWN2_002659"
     CDS             <1..831
                     /locus_tag="ACHWN2_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8932138.1"
                     /translation="TFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDELATFFT
                     WTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNPSSHNE
                     RIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSVCQKFKWLLPDDDEMCQWFWT
                     RLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRINHAWH
                     QKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLEHKRRK
                     "
     gene            941..1813
                     /locus_tag="ACHWN2_002660"
     CDS             941..1813
                     /locus_tag="ACHWN2_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFR8932139.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            2147..5269
                     /gene="agn43"
                     /locus_tag="ACHWN2_002661"
                     /gene_synonym="flu"
     CDS             2147..5269
                     /gene="agn43"
                     /locus_tag="ACHWN2_002661"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFR8932140.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            5390..7906
                     /locus_tag="ACHWN2_002662"
     CDS             5390..7906
                     /locus_tag="ACHWN2_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932141.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            7982..8437
                     /locus_tag="ACHWN2_002663"
     CDS             7982..8437
                     /locus_tag="ACHWN2_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFR8932142.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            8580..8669
                     /locus_tag="ACHWN2_002664"
     CDS             8580..8669
                     /locus_tag="ACHWN2_002664"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR8932143.1"
                     /translation="MTAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            8848..9666
                     /locus_tag="ACHWN2_002665"
     CDS             8848..9666
                     /locus_tag="ACHWN2_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR8932144.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            10008..10481
                     /locus_tag="ACHWN2_002666"
     CDS             10008..10481
                     /locus_tag="ACHWN2_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR8932145.1"
                     /translation="MKTLSQNTTSSACAPETDLQQLVATLVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            10497..10973
                     /gene="radC"
                     /locus_tag="ACHWN2_002667"
     CDS             10497..10973
                     /gene="radC"
                     /locus_tag="ACHWN2_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR8932146.1"
                     /translation="MQQLSFLPGEMTTRERSFILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            11060..11281
                     /locus_tag="ACHWN2_002668"
     CDS             11060..11281
                     /locus_tag="ACHWN2_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFR8932147.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            11361..11738
                     /gene="cbeA"
                     /locus_tag="ACHWN2_002669"
     CDS             11361..11738
                     /gene="cbeA"
                     /locus_tag="ACHWN2_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFR8932148.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            11785..12162
                     /locus_tag="ACHWN2_002670"
     CDS             11785..12162
                     /locus_tag="ACHWN2_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFR8932149.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            12159..12647
                     /locus_tag="ACHWN2_002671"
     CDS             12159..12647
                     /locus_tag="ACHWN2_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFR8932150.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            12667..12864
                     /locus_tag="ACHWN2_002672"
     CDS             12667..12864
                     /locus_tag="ACHWN2_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFR8932151.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            12949..13791
                     /locus_tag="ACHWN2_002673"
     CDS             12949..13791
                     /locus_tag="ACHWN2_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR8932152.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            14568..14813
                     /locus_tag="ACHWN2_002674"
     CDS             14568..14813
                     /locus_tag="ACHWN2_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932153.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
BASE COUNT         3722 a         3903 c         3913 g         3339 t
ORIGIN      
        1 acttttggta ctactgaaga agaagcactc aataacgaaa ttaatgagct tgaaattatt
       61 gccgggcagg caatactgcc tgaagagagt tttctctggt tgaaaactga cgaacttgcg
      121 actttcttta cctggactac tatttatctg tcgaaggaag accatacaaa attcccgaaa
      181 ataaaccata attcatcagg aataaaaaac ggaattgaag tcaggaaaat acgaacagtt
      241 accgatgaaa cgttaaacac ttacaaaaaa agtaaccttc cggataatcc atcatcccat
      301 aacgaacgaa taaaagtaat catttcatat tttgactcct gccattcttt aattaataaa
      361 aatgcaaaaa gcatatacct cgaggaactc aggcaggcgt ggcttaaaaa gaaaagcgtt
      421 tgtcaaaagt ttaagtggct ccttcctgac gatgatgaaa tgtgtcagtg gttctggaca
      481 agactgaaac aaaaacagga tgaaaaacat ccagtacata acccagtgac ccggtggttc
      541 ataccatctt caacgaaaga atattatctt gcctctcttg tcgcctttgc tttatggaaa
      601 gaagcgcctg ataccattga actgttcaga acacgaataa atcatgcctg gcatcagaaa
      661 aaacaaaagg acaaatatcg gagtgatggt aaaaaggcca ttaatataca tataagaagc
      721 aatgtgaaag aaatgcttga tgagctcaga actgtttacg gtctgagaac aggagagttt
      781 tttgaaatgc tgattacaga gaaatatctt gaacacaagc gaaggaagta attcattacc
      841 tgttacattc taaataatct cagacagata ttatctccgt gaatcccccg ccacctttcc
      901 ggtgcgcggg gttttgtctt ttttcaccgg gaatacatgt atgaatccgt ctgatgccat
      961 tgaggcaatt gaaaaaccgc tctcctccct gccttactca ctttcccgtc acatcctgga
     1021 acatctgcgc aaactcacca gtcacgaacc cgtgattggc attatgggta aaagcggggc
     1081 cggtaaatct tcactctgta atgcactgtt tcagggggag gtcacaccgg tcagtgatgt
     1141 tcacgccggc acccgggaag tgcagcgctt ccgtctgagt ggtcacggtc acagcatggt
     1201 tatcactgac ctgcccgggg tgggcgagag ccgggacagg gatgcagagt atgaagccct
     1261 gtaccgtgac attctgcctg aactggacct ggtgctgtgg ctgattaaag ccgatgaccg
     1321 ggccctgtct gtggatgagt atttctggcg acacatcctg cagtgcggac atcagcaggt
     1381 gctgtttgtg gtgacgcagg ctgacaaaac ggaaccctgc catgaatggg atatggcagg
     1441 tattcagcct tctcccgcac aggaacagaa cattcgtgag aaaacggagg cggtattccg
     1501 tctgttccgg cccgtacatc cggttgttgc cgtatcggcc cgcaccggct gggaactgga
     1561 tacgctggtc agtgcgctca tgacagcgct tcccgaccat gccgccagcc ccctgatgac
     1621 ccgactgcag gacgagctgc gcacggagtc tgtccgctct caggcccgtg aacagtttac
     1681 cggtgcggtg gaccggatat ttgacacagc ggagagcgtc tgtgttgcct ctgttgtccg
     1741 tacggccctg cgcgctgttc gtgacaccgt ggtctctgtt gcccgcgcgg tatggaactg
     1801 gatcttcttc tgaacctgtt gtggatgatg tcctccctgc ctctgagtct gctcacaaaa
     1861 gcgctgtttt cgttactgtc tctcttgtcc gtgcaataga ctgataatag aataaaacga
     1921 tcgataccta ttttaccgat cgttaatatc gatcgttatg ctaataataa ctcctgttag
     1981 caacgtgcgc agatacacac agacatgaga ttcagggaac aacagagcca cacgtcagaa
     2041 acttccgtca gccggacctc cggcactgta accctttacc tgccggtatc cacatctgtg
     2101 gataccggct tttttattca ccctcactct gattaaggaa atgctgatga aacgacatct
     2161 gaatacctgc tacaggctgg tatggaatca cattacgggc gctttcgtgg ttgcctccga
     2221 actggcccgc gcacagggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcggt
     2281 cacgtcactc ccggtgctgg ctgctgacat cgttgtgcac ccgggtgaaa cagtgaatgg
     2341 cggaacactg gtaaaccatg acaaccagtt tgtatccgga acagctgatg gcgtgactgt
     2401 cagtaccggg cttgagctgg ggccggacag tgacgaaaac accggcgggc aatggataaa
     2461 agcgggtggc acaggcagaa acaccactgt caccgcaaat ggtcgtcaga ttgtgcaggc
     2521 aggaggaact gccagtgata cggttattcg tgatggcgga gggcagagcc ttaacggact
     2581 ggcggtgaac accacgctgg ataacagagg tgagcagtgg gtacacgggg gagggaaagc
     2641 tgccggtaca attattaacc aggatggtta ccagaccata aaacatggcg gactggcaac
     2701 cggaaccatc gtcaacaccg gtgcagaagg tggtccggag tctgaaaatg tgtccagcgg
     2761 tcagatggtc ggagggacgg ctgaatccac caccatcaat aaaaatggcc ggcaggttat
     2821 ctggtcttcg gggatggcac gggacaccct catttacgcc ggtggtgacc agacggtaca
     2881 cggagaggca cataacaccc gactggaggg gggtaaccag tatgtacaca acggtggcac
     2941 ggcaacagag acgctgataa accgtgatgg ctggcaggtg attaaggaag gaggaactgc
     3001 cgcgcatacc accatcaacc agaaaggaaa gctgcaggtg aatgccggcg gtaaagcgtc
     3061 tgatgtcacc cagaacacgg gcggtgcact ggttaccagt acagctgcaa ccgtcaccgg
     3121 cacaaaccgc ctgggagcat tctctgttgt ggagggtaaa gctgataatg tcgtactgga
     3181 aaatggcgga cgcctggatg tgctgaccgg acacacagcc actaataccc gcgtggatga
     3241 tggcggaacg ctggatgtcc gcaacggtgg caccgccacc accgtatcca tgggaaatgg
     3301 cggtgtactg ctggccgatt ccggtgccgc tgtcagtggt acccggagcg acggaaaggc
     3361 attcagtatc ggaggcggtc aggcggatgc cctgatgctg gaaaaaggca gttcattcac
     3421 gctgaacgcc ggtgatacgg ccacggatac cacggtaaat ggcggactgt tcaccgccag
     3481 gggcggcaca ctggcgggca ccaccacgct gaataacggc gccatactta ccctttccgg
     3541 gaagacggtg aacaacgata ccctgaccat ccgtgaaggc gatgcactcc tgcagggagg
     3601 cgctctcacc ggtaacggca gcgtggaaaa atcaggaagt ggcacactca ctgtcagcaa
     3661 caccacactc acccagaaag ccgtcaacct gaatgaaggc acgctgacgc tgaacgacag
     3721 taccgtcacc acggatgtca ttgctcagcg cggtacagcc ctgaagctga ccggcagcac
     3781 tgtgctgaac ggtgccattg accccacgaa tgtcactctc gcctctggtg ccacctggaa
     3841 tattcccgat aacgccacgg tgcagtcggt ggtggatgac ctcagccatg ccggacagat
     3901 tcatttcacc tccacccgca cagggaagtt cgtaccggca accctgaaag tgaaaaacct
     3961 gaacggacag aatggcacca tcagcctgcg tgtacgcccg gatatggcac agaacaatgc
     4021 tgacagactg gtcattgacg gtggcagggc aaccggaaaa accatcctga acctggtgaa
     4081 cgccggcaac agtgcgtcgg ggctggcgac cagcggtaag ggtattcagg tggtggaagc
     4141 cattaacggt gccaccacgg aggaaggggc ctttatccag gggaataagc tgcaggccgg
     4201 tgcctttaac tactccctca accgggacag tgatgagagc tggtatctgc gcagtgaaaa
     4261 tgcttatcgt gcagaagtcc ccctgtatgc ctccatgctg acacaggcaa tggactatga
     4321 ccggattctg gcaggctccc gcagccatca gaccggtgta agcggtgaaa ataacagcgt
     4381 ccgtctcagc attcagggcg gtcatctcgg tcacgataac aacggcggta ttgcccgtgg
     4441 agccacgccg gaaagcagcg gcagctatgg cttcgtccgt ctggagggtg acctgctcag
     4501 aacagaggtt gccggtatgt ctgtgaccgc gggggtatat ggtgctgctg gccattcttc
     4561 cgttgatgtt aaggatgatg acggctcccg tgccggcacg gtccgggatg atgccggcag
     4621 cctgggcgga tacctgaatc tgatacacaa cgcctccggc ctgtgggctg acattgtggc
     4681 ccagggaacc cgccacagca tgaaagcctc atcggacaat aacgacttcc gcgtccgggg
     4741 ctggggctgg ctgggttcgc tggaaaccgg tctgcccttc agtatcactg acaatctgat
     4801 gctggagccg caactgcagt acacctggca gggactctcc ctggatgacg gccaggataa
     4861 cgccagttat gtgaagttcg ggcatggcag tgcacaacat gtgcgtgccg gcttccgtct
     4921 gggcagccac cacgatatga actttggtaa aggcacctca tcccgtgaca ccctgcgcgg
     4981 cagtgccaaa cacagtgtgc gtgaactgcc ggtgaactgg tgggtacagc cttctgttat
     5041 ccgcaccttc agctcccggg gggacatgag catgggtaca gccgcagccg gcagtaacat
     5101 gacgttctca ccgtcacaga atggtacgtc actggacctg caggccggac tggaagcccg
     5161 tgtccgggaa aatatcaccc tgggcgttca ggccagttat gcccacagca tcaacggcag
     5221 cagcgctgaa ggttataaca gtcaggccac actgaatgta accttctgat aattctgcat
     5281 tgcctctctg tggtcccggt catgatgacc gggacccgga cgggggaaaa cgtttcagtg
     5341 ccacattcac tggcattcac aataacatga tattcatcac ggagtgacta tgttacagat
     5401 agtcggcgcg ctgattctgc tgatcgcagg atttgccatt cttcgccttt tgttcagagc
     5461 attaaccagc acagcgtctg cgctggcagg gttcatattg ctgtgtctgt tcggtccggc
     5521 tttactggct ggctatatca ctgaacgcat aacccggtta ttccatattc gctggctggc
     5581 aggcgtattt ctgacgattg ccggaatgat catcagcttc atgtggggac ttgatggtaa
     5641 acatatcgca ctggaggctc atacttttga ctctgtaaaa tttattctga ccaccgctct
     5701 cgccgctggt ctgctggctc ttcccgtgca gataagaacc attcagcaga acgggctcac
     5761 acctgaagat atcagcaagg aaattaacgg gtattactgc tgtttttata ctgctttttt
     5821 ccttatggcg tgttctgcat acgcaccatt gatcgcattg cagttcgata tttcaccctc
     5881 actgatgtgg tggggcgggt tgttgtactg gctggctgca ttagtgacgc tgctatgggc
     5941 ggccagccag atccaggcgc tgaaaagact gaccagtgcc atcagccaga cactggaaga
     6001 acaaccggtg ctcaacagta aatcgtggct gagcagtttg caaaacgatt acagccttcc
     6061 tgaaacgctg acggagcgca tctggctgac actcatttca caacggattt cccggggaga
     6121 actgagggaa tttgaactgg cagacggaaa ctggttactg aacaatgcct ggtatgaaag
     6181 aaacatggca gggtttaacg aacagttgaa agagaacctg tcattcacac ctgatgaact
     6241 gaaaacgctc ttccggaacc gcctgaattt atcaccggaa gcgaatgacg attttctcga
     6301 tcgttgcctg gacggcggtg actggtaccc cttttcagaa ggccgccgtt ttgtatcatt
     6361 ccaccacgtg gatgagcttc gtatctgtgc ctcctgcggg ctgacagaag tacatcatgc
     6421 cccggaaaat cataagccgg ctccggaatg gtactgctcc tctctttgtc acgaaacaga
     6481 aacactgtgt caggacattt atgaacgttc ttacaccggt tttatttccg atgcaacggc
     6541 gaatggtctg attctcatga aactgccgga aacctggagt acaaatgaga aaatgtttgc
     6601 ttccggaggg cagggacatg ggtttgccgc tgaacgggga aaccatattg tcgacagagt
     6661 ccgtctgaaa aacgcacgga tcctcggtga taataatgcc agaaatggag cagacagact
     6721 ggtcagcgga acagaaatcc agacgaaata ttgttcaact gcagcccgta gcgtcggtgc
     6781 ggcattcgac ggacaaaacg gacagtatcg ttacatggga aatcatggtc ccatgcaact
     6841 ggaagtcccc cgtgatcagt atgccggcgc tgtggaaacc atgaagaata agatccgcga
     6901 aggtaaagta cccggtgtaa ccgatcccgc agaagcgtcc cggctgattc gtcggggaca
     6961 tctgacttat acccaggccc gtaatatcac ccggttcggg accatcgaat cggtcactta
     7021 tgatattgcc gaggggtcgg ttgtcagtct ggcggccgga gggatcagtt ttgccctgac
     7081 ggcatcggtc ttctggctca gcaccggcga tcgcgatgct gccctgcaga cagctgctgt
     7141 ccaggcagga aaaaccttca cccgcacact ggctgtctac gtcacaaccc agcaacttca
     7201 ccggctcact gttgttcagg gtatgctgaa gcatattgat ttttcgacgg ccagcccgac
     7261 tgtccggcag gcgcttcaga aggggaccgg tgcaggaaat atcagtgccc tgaacaaagt
     7321 gatgaagggt acgctggtga catctctggc actggtagct gtcacaacca gccctgacat
     7381 gatcaaaatg ttgcggggac ggatctccgg tgcgcagttc atcaggaatc ttgccgtggc
     7441 atcttccggt gtggcaggtg gtgctgtcgg gtcagtggcg ggcgggatat tgttcagtcc
     7501 actgggacca tttggtgcac tgacagggcg tgtggttggc ggtgttctgg ggggaatgat
     7561 tgcctccgct gtatcaggaa aaattgccgg agcgctggtt gaagaagatc gcgtcaaaat
     7621 tctggcaatg attcaggagc aggtgacatg gcttgccggc agtttcctgc tgaccggaca
     7681 tgagattgaa aatctgaacg cgaatctggc ccgtgttatc gatcagaatg ctctggagat
     7741 cattttcgcc gccggtatac aacaacgggc cgcgaccaat atgttaatca aaccactggt
     7801 ggtcagtatc atcaggcaac gccccgtcat ggaatatgat gcatcccatc tcggcaagat
     7861 ggttaaccgg ctggaagaag cattcccccc ggagctcccg gcataacgtg tgttctgctc
     7921 cttttaggtt ttatcacctg atttgccagt gtcatcgtca tgactcagag aggtaaatac
     7981 catgattcac ctgttcaaaa cctgcatgat taccgccttc attctggggt taacgtggtc
     8041 tgccccactc cgggcacagg atcaacgtta catcagtata cgcaatacag atacgatatg
     8101 gctcccggga aatatttgtg cttaccagtt ccggctggat aatggcggaa acggtgaagg
     8161 atttggcccc ctcaccatca ctctgcaact caaagacaaa tatggtcaga cgctggtgac
     8221 cagaaaaatg gaaacggaag cttttggtga cagtaatgcc acgcgaacca cagacgcatt
     8281 tctggaaacg gagtgcgtgg aaaatgtcgc cacaaccgaa atcattaaag caactgaaga
     8341 aagtaacggc catcgtgtca gtctgccgtt atcggttttc gatccccagg actaccatcc
     8401 actgctgatt accgtttccg gaaaaaatgt taactgatcc ctgaagtcag tttcatgctt
     8461 cagcctctgc cacaacgtat ggcagaggct atttatcagg agaaaccaga tatgccgggt
     8521 tacacacaga gtatgtcctg gctgaaggat ctttctcata cgggcaggcg gttgctgtca
     8581 taaccgctta ccgcaatgtg tttatccagg atgacccggg aatgcatttc cgtcgggtta
     8641 tccgcaacgc ggaaggtcag cgacgctgac gcagccggaa ctctgaagct gatgccggaa
     8701 aacagcttaa tgcgtggctc gccagtggag ggcttctcag acaataaact tcattgattt
     8761 catccatcag gccgcgtctt ctccgggaga cgcggctttt ttcatttgtt gcaccagtaa
     8821 atcttaacca ccgataagga gcaaagtatg caattagtca gtcgttttgg ttatgcgaac
     8881 cagatacgcc gtgaccgtcc gctgacacat gaagagctga tgcaccatgt gccggggatt
     8941 ttcggggaag agaaacacac gtcccgaagc cagaactata cgtacatccc caccatcacc
     9001 gtactggaaa gtctgcagcg ggaaggcttc cagccctttt tcgcctgcca gacccgtgtg
     9061 cgcgacccgg gccgccgggg atacacaaaa cacatgctgc gtctgcggcg ggccggagag
     9121 ataaacggag aacatgtccc tgaaattatt ctgctcaact cccatgacgg tacctccagc
     9181 taccagatgc tgccgggtta cttcagattc gtctgccaga acgggtgtgt ctgtggtcag
     9241 tctctggggg aagtgcgtgt tccgcaccgg ggaaacgtgg tggagaaagt tattgaaggg
     9301 gcttacgaag tggtgggcgt gtttgaccgg attgaggaga aacgtgatgc catgcagtcg
     9361 ctggtcctgc cgccaccggc acgccaggcg ctggcacagg cggcactgac ttaccgttat
     9421 ggtgacgaac atcagcccgt caccaccgcc gacattctga cgccacgacg ccgggaggat
     9481 tacggtaagg acctgtggag tacttatcag accatccagg agaatatgct gaaaggcggg
     9541 atttccggcc gcagtgcaaa aggaaaacgt atccacaccc gtgccattca caacatcgac
     9601 accgatatta agctcaaccg cgcattgtgg gtgatggcag aaacgctgct ggagagcctg
     9661 cgctgatgca gcatgtccct gaaggcgtga ttcaggcacc agaatcaccc gacgccccgg
     9721 aggcgctgcc ggggtgtcgt tccatttctg atgatgacct gtgcgcctgg tgtacccgtc
     9781 tgctttaccg tcccggagaa atcagtctgt gcagactgag tatacaggca atggcatctg
     9841 gccatccgtc tgtgaccgga acggttacgc gtacggctgt ccggaatttc agccgaatat
     9901 cattcatccc tgatattacc gggtcatcac ggtcagtgat gaacgtgatg ttgccagctc
     9961 ccgaccttta ctccctctca tttattcaca ttacaaggat ttcatacatg aaaacattgt
    10021 ctcagaacac cacctcttcg gcctgtgcac cggagactga cctgcaacaa ctggttgcca
    10081 ccctcgtccc tgatgaacag cgcatcagct tctggccgca gcactttggc ctcattccac
    10141 agtgggtgac cctggagccc cgtgtcttcg gctggatgga ccgtctgtgc gaagactact
    10201 gcggtggtat ctggaatctg tacaccctga acaacggcgg ggcatttatg gcacccgaac
    10261 cggatgacga tgatgacgaa acatgggtac tgttcaatgc catgaacggt aaccgcgctg
    10321 aaatgagtcc ggaagctgcc ggcattgctg cctgtctgat gacgtacagt catcatgcct
    10381 gtcgtacgga gtgttatgcc atgacggtcc attattaccg gctgcgggat tacgccctgc
    10441 agcatccgga atgcagcgcc attatgcgca tcatcgactg aatggtggca gggacaatgc
    10501 agcagctttc ctttctgccc ggagaaatga caaccagaga gcgcagcttc attctgcggg
    10561 ccctgaaaac cctggaccgc catcttcatg aacccggcgt ggccttcacc tccacgcatg
    10621 cggcacggga atggctgatt ctgaacatgg cgggactgga gcgtgaagag ttccgggtgc
    10681 tgtatctgaa caaccagaat cagctgattg ccggtgaaac cctcttcacc ggcaccatca
    10741 accgtacgga agtccatccc cgggaagtga ttaaacgcgc cctgtaccac aatgccgctg
    10801 ccgtggtgct ggcgcacaat cacccgtccg gtgaagtccc ccccagcaag gcagaccgcc
    10861 ttatcactga gcgtctggta caggcactgg ccctggttga tatccgggta ccggaccatc
    10921 tgatagtcgg tggcagccag gttttctcct ttgcggaaca cggtctgctt taacccgtca
    10981 ccgtcacaat caccttcata tcacttcagt ttctctttct cagctgtttc ttactttcac
    11041 attcaggagg actattctca tgaaaatcat cacccgtggt gaagccatgc gtattcaccg
    11101 tcagcatcct gcatcccgtc tttttccgtt ctgtaccggt aaataccgct ggcacggtag
    11161 cacggataca tataccggcc gtgaagtgca ggatattccc ggtgtactgg ctgtgtttgc
    11221 agaacgccgt aaggacagtt ttggtccgta tgtccggctg atgagcgtca ccctgaactg
    11281 agtctggaag ggaattctga tgagcagaat tatcgccact accggaccat tcttaaccga
    11341 ttttctgtga ggactttatc gtgtcagaca cactccccgg gacaacgcct cccgacgaca
    11401 atcacgaccg cccctggtgg gggctgccct gcaccgtgac gccctgtttc ggggcccgac
    11461 tggtacagga gggtaaccgc ctgcattacc ttgcagaccg cgccggtatc agaggcaggt
    11521 tcagcgacgt agatgcgtac cacctggacc aggcctttcc gctgctgatg aaacaactgg
    11581 aactcatgct cactggcggt gaactgaatc cccgccatca gcataccgtc acactgtatg
    11641 cgaaagggct gacctgcgaa gccgacaccc tcggctcctg tggttacgtt tatctggctg
    11701 tttatccgac accggcagcg cccgcaacca ccgtataaca ccggatatct cccctttata
    11761 accacctgtt acagagaact taagatgaat acattacccg acactcacgt acgggaggca
    11821 tcgggctgcc cgtctcccat caccatctgg cagacactgc tcacccgact gctggaccag
    11881 cactatggcc tcacgctgaa tgacacaccg ttcgctgatg aacgtgtgat tgagcagcat
    11941 attgaggcag gaatttcact gtgtgacgcg gtgaactttc tcgttgaaaa atacgcgctg
    12001 gtacgtaccg accagccggg attcagcgca ggagccccgt cgcagttaat caacagcatt
    12061 gatattctcc gggcacgccg ggcaaccggc ctgatgaccc gcgataacta cagaacggta
    12121 aataacatta cccgggggaa gcatccggaa gcgaaacaat gaaactggcc ctgacgctgg
    12181 aagccgacag cgttaacgtg caggcactga acatggggcg cattgtcgtt gacgtcgatg
    12241 gtgttaatct cagtgaactg attaacaagg tctctgaaaa cggttattca ctccgcgtgg
    12301 tcgataaatc cgaccaacac gcaaccagta caccaccacc gttaacaacc cttacctgca
    12361 tacgttgcag caccgcacat atcacggaaa cggacaacgc ctggctgtac tcgctgtcac
    12421 accagactaa tgacgacggc gaaacagaat ggattcattt tacaggcagc ggctatctgt
    12481 tacgtaccga tgcatggtcg tacccggttc tgcggcttaa acgcctgggc ctgtccagaa
    12541 cgttccgtcg tctggtcgtc actctcaccc ggcgttatgg cgtcagtctc attcatctgg
    12601 acgccggcgc agaatgtctg ccggggttcc ccactttcga ctggtaatca ggacaaaaga
    12661 cactggatga aattattaac cacagaaacg gcgctggata ttctgattgc gtggctgcag
    12721 gacaatattg actgcgaatc agggattatc ttcgacaacg atgaggataa aacggattcg
    12781 gcagcactgc tgccctgtat tgaacaggcc agggaagata tccgtaccct gcgtcagctg
    12841 cagtttctgc aacagaaccg gtgagtctta ctcaccagac ttcattccac tgacgccagc
    12901 ctgaatacgg ctggcgtttt cgtttatctg caaaaaggaa tatcgattat gtctgaaatc
    12961 acagcctctc gtccggaagt ggtcaacggg catacggacg ttatctgctc aaccagtatc
    13021 cgccacattc tggctgtacg aaagagtaca ttgctgcaaa tcgacacact tatccgacaa
    13081 ctggctgaag tatcagcaat gacagaaagt attagaggta aaacggctct ggactgggcc
    13141 atgaaacagg attttcgctg cggttgctgg ctgatggaga aaccggaaac cgcaatgaaa
    13201 gccatcacac gcaatcttga tcgcgaactc tggcgtgatc tgatgcaacg ttccgggatg
    13261 ctgagtctga tggatgcgca gacccgggac acatggtaca ggtcactgga atatgataat
    13321 tttccggaaa tcagcgaagc gaacattctg agcacatttg aacaactgca ccagaacaaa
    13381 gatgaagtgt ttgaacgggg agtgatcaac gtcttcaaag ggctgagctg ggattacaaa
    13441 accaactcac cctgtaaatt tggcagtaaa attatcgtca acaacctggt gaggtgggac
    13501 cggtgggggt ttcatcttat caccgggcaa cagactgacc gactcgtcga cctggaaaga
    13561 atgctacatc tgttcagcgg caaaccgatc cccgacaacc gggaaaacat cactattcgt
    13621 ctggatggtc acatccagtc cgttcagggt aaagagcgct atgaagacga gatgttcatc
    13681 attaaatact ttaagaaggg atctgcacac atcactttca aaaggctgga actgattgac
    13741 agaattaacg atataatagc caggtacttt ccttctgtgc tctcagcctg atcccgagag
    13801 tgattccctt tcgatatcaa aagggactgc gggtataatt gagggtacat ttttaatcaa
    13861 accaaacaaa ttgcattaat atcaatatga taaaagaaat tttcgattcc gagtccggca
    13921 ccactattta gaagaaccag cctacgggct ggttttttgc tttctggggt tctggtagat
    13981 atccggtcac tttgaatgct gatgtcactg ctttacctct tccacccagc ctgaacattt
    14041 cttctaagta gcccccttct ggtcactgtg gccttttctg tcaaacttca aatccccaat
    14101 agacactatt tccacatgag cacctggcta acggattcat tactgcacgg atacatccgg
    14161 tctttgtccg gtctgaagta aaacgcatac cacctgcgtt ggcccctcct cagtcccccc
    14221 caacatcaaa ccgaacattt ttcactctga ttgatttagc tttagtcaac ttttgggtgt
    14281 tatgcctact atatcaatat gatccatagg ggattatggc cagactaccg gtaataccga
    14341 gagacaggat ctcaatcgga gatagaacgt tctgcaaaac ggggcttaca gaaaatccct
    14401 aaatgacctg caacctccag cccattgcat attatgataa cagtgaatat tttggttaag
    14461 tcgcgccccg caaaaggtgg aaagcaattt aacaaattga attaaaaggg tttaaccgca
    14521 aaatggacgc aacaggcaac acgacagtca cctgcctgag tcacaaaatg aagtacaaag
    14581 aagtcgcctg cggtaagcag cggatgcact ggctggcgct gcagttcgtg gatgagaaca
    14641 acaacccagt ttccggtctc aacgtgcagc tggagtatca tccacgggca atggcggcag
    14701 agctggctct gtgggccaga ggcagacaca ctcagtttga ccccacaccg ccgcccccac
    14761 ctgtcggtgt cactgacagt cagggtctgg tacggtttga tgacttgtac tgaattaaag
    14821 cacctaaaac aaacatttat atcgcaacag ttaaatagaa tggataatcg gtaataa
//
LOCUS       DAZSNO010000092        14479 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZSNO010000092 DAZSNO010000000
VERSION     DAZSNO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14479
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.092"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..116
                     /locus_tag="ACHWN2_002675"
     CDS             <1..116
                     /locus_tag="ACHWN2_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932154.1"
                     /translation="TISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(113..379)
                     /locus_tag="ACHWN2_002676"
     CDS             complement(113..379)
                     /locus_tag="ACHWN2_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR8932155.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="ACHWN2_002677"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="ACHWN2_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR8932156.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="ACHWN2_002678"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="ACHWN2_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="ACHWN2_002679"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="ACHWN2_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR8932157.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="ACHWN2_002680"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="ACHWN2_002680"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR8932158.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="ACHWN2_002681"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="ACHWN2_002681"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR8932159.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(8885..9058)
                     /gene="ynaL"
                     /locus_tag="ACHWN2_002682"
     CDS             complement(8885..9058)
                     /gene="ynaL"
                     /locus_tag="ACHWN2_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR8932160.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="ACHWN2_002683"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="ACHWN2_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR8932161.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(10330..10377)
                     /gene="ydaN"
                     /locus_tag="ACHWN2_002684"
     CDS             complement(10330..10377)
                     /gene="ydaN"
                     /locus_tag="ACHWN2_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR8932162.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="ACHWN2_002685"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="ACHWN2_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="ACHWN2_002686"
                     /pseudo
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="ACHWN2_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12641..14095)
                     /locus_tag="ACHWN2_002687"
     CDS             complement(12641..14095)
                     /locus_tag="ACHWN2_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFR8932163.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14479)
                     /locus_tag="ACHWN2_002688"
     CDS             complement(14092..>14479)
                     /locus_tag="ACHWN2_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR8932164.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3860 a         3651 c         3418 g         3550 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaacttctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt tttgtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatctggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccactgtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgcta
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgca
//
LOCUS       DAZSNO010000093        14345 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZSNO010000093 DAZSNO010000000
VERSION     DAZSNO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14345
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.093"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..3051
                     /locus_tag="ACHWN2_002689"
                     /pseudo
     CDS             1..3051
                     /locus_tag="ACHWN2_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="ACHWN2_002690"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="ACHWN2_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="ACHWN2_002691"
     CDS             6768..7175
                     /locus_tag="ACHWN2_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932165.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="ACHWN2_002692"
     CDS             7717..8805
                     /locus_tag="ACHWN2_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFR8932166.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="ACHWN2_002693"
     CDS             8816..11095
                     /locus_tag="ACHWN2_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFR8932167.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12225
                     /locus_tag="ACHWN2_002694"
                     /pseudo
     CDS             11088..12225
                     /locus_tag="ACHWN2_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            12222..14252
                     /locus_tag="ACHWN2_002695"
     CDS             12222..14252
                     /locus_tag="ACHWN2_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932168.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASVDAFHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVG
                     SGIAGEAKLDHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSP
                     AATRRLFSFQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPL
                     RQPGIVALREYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEG
                     EDNLLTLSLPASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKT
                     RAWALDAETAPVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQA
                     ELLANDLTAVGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3549 a         3769 c         3662 g         3365 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggccc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg accacaagcg tcgttgatct cacggcagac
    11881 gttgccgatc cggtagagtt attaatgaaa gtgcaattag gcggcggaac caatatcgcc
    11941 agtgccatgg agtatggtcg gcaacttatt gaacaacctg caaaaagcgt cattatcctc
    12001 gtgagcgatt tttatgaagg gggttcatca tcattgctga cgcaccaagt gaaaaagtgt
    12061 gtccagagcg gcatcaaagt gctgggactg gcagcgctcg atagcacagc aacaccttgc
    12121 tatgaccgcg atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca
    12181 ccgggcgagc tggcatcatg gcttgcggag aatcttcagt catgaattca ctacgtccgg
    12241 aattattaga actgacaccg caggcactga cggcgttaag caatgccggt tttgttaagc
    12301 gcagccttaa ggaactggaa aatggcaacg tcccggaaat cagccatgag aacagcgctt
    12361 taatcgccac cttcagtgac ggtgtccgta cccagctggc gaacggccag gcactgaaag
    12421 aggctcagtg cacctatggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa
    12481 gttatcaacg actttgtgct accgcccagc ctacgggaaa agaagaggaa tgggatccgg
    12541 caatctggct ggaagaactg gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc
    12601 tggtcgctaa aagcatcacc attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc
    12661 gcttaccgat gagcgatgtg cgtttttatt cccgcagcag tattcgtttc gcccgttgtg
    12721 attgtattga aggcacactt tgcgaacatg tcgtactggc agtacaggcc tttgtccagg
    12781 caaaagcgca gcaagcggaa ttgactcatt taatctggca gatgcgcagc gaacacgtca
    12841 catcatctga cgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac
    12901 aactaagcca ggcattatgg ctgagcggca tcagccagcc gcttattcac tacgaggccg
    12961 ctttcagccg cgcgcagcaa gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc
    13021 tacggcaact gcgcgccagc gttgatgcct tccacgcccg cgccagcgtt gatgccttcc
    13081 acgcccgcgc cagccactat catgccggag aatgcttacg tcagcttgcg gcattaaaca
    13141 gtcgattaaa ttgcgcacaa gagatggccc ggagcgacag tgttggtgaa gttcctcctg
    13201 tgccgtggcg cacggtcgtt ggctctggca ttgccggaga agcaaagctt gatcatctgc
    13261 ggctggtgtc tttaggtatg cgttgctggc aggatattga gcattatggt ttacgcatct
    13321 ggtttaccga tcccgacacc ggcagtattt tgcacctttc gcgcagttgg ccgcgaagtg
    13381 aacaggaaga ctcaccggca gctacgcgtc ggctgtttag ttttcaggct ggcgcactgg
    13441 cgggtgggca aattgtttca caagcagcaa aacgcagtgc cgatggcgag ctgctgttag
    13501 ctacccgcaa ccgcttaagc agcgttgtac cgctgtcgcc tgatgcctgg caaatgttga
    13561 gcgcgccgtt acgccagccg ggcattgtgg ctttgcggga atatttacgc cagcgtcccc
    13621 ccgcctgcat acggcctctt aatcaggtcg ataacttatt tattctgccg gtcgctgagt
    13681 gtatttcgct cggttgggat agcagccgcc agacgctgga tgcgcaggta atcagcggcg
    13741 aaggtgaaga taatctgctg acgttatcac tgccagcctc agccagcgcg ccttatgcca
    13801 ttgaacgcat ggcggcgctt ttgcaacaaa cagacgaccc cgtgtgtctg gtttctggct
    13861 ttgtcagttt tgttgatggg caattgacac tggaaccaca ggtaatgatg acaaagactc
    13921 gtgcctgggc gctggacgca gaaactgcgc ctgtggtggt atcactacct tctgccagcg
    13981 ttctgcctgt gccgtctact gctcatcagt tgctgatgcg ctgccaggcg ttacttattc
    14041 aactgctcca taacggctgg cgctatcagg aacagagcgc tattaatcag gcagagttgc
    14101 tggcgaatga cctcaccgcg gtgggttttt atcggctggc acatgtgttg gcacaatttc
    14161 gtaatacaga aagcgaggca cgggtagaag caatgaataa cggtgttttg ctttgcgaac
    14221 aattattccc catgcttcag caacaaggat gaaatagtgc tttttactaa gagttctact
    14281 ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc ctggtgagag
    14341 tcacc
//
LOCUS       DAZSNO010000094        14338 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZSNO010000094 DAZSNO010000000
VERSION     DAZSNO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14338
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.094"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..185
                     /locus_tag="ACHWN2_002696"
     CDS             <1..185
                     /locus_tag="ACHWN2_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR8932169.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="ACHWN2_002697"
     CDS             complement(210..401)
                     /locus_tag="ACHWN2_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR8932170.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="ACHWN2_002698"
     CDS             complement(506..742)
                     /locus_tag="ACHWN2_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFR8932171.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="ACHWN2_002699"
                     /pseudo
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="ACHWN2_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="ACHWN2_002700"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="ACHWN2_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="ACHWN2_002701"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="ACHWN2_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR8932172.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYRRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="ACHWN2_002702"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="ACHWN2_002702"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR8932173.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="ACHWN2_002703"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="ACHWN2_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR8932174.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="ACHWN2_002704"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="ACHWN2_002704"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR8932175.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="ACHWN2_002705"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="ACHWN2_002705"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR8932176.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="ACHWN2_002706"
     CDS             complement(10860..12233)
                     /locus_tag="ACHWN2_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8932177.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="ACHWN2_002707"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="ACHWN2_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR8932178.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="ACHWN2_002708"
     CDS             complement(13344..13583)
                     /locus_tag="ACHWN2_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR8932179.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="ACHWN2_002709"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="ACHWN2_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR8932180.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="ACHWN2_002710"
     CDS             13960..14247
                     /locus_tag="ACHWN2_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR8932181.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3759 a         3510 c         3684 g         3385 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggttgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaactgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcca ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctacgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtat
     1561 aggtcgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtctct gcactcaaac tcatggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagtatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgtcggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac tgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgcagat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagagg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaatta
//
LOCUS       DAZSNO010000095        14294 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZSNO010000095 DAZSNO010000000
VERSION     DAZSNO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14294
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.095"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..79
                     /locus_tag="ACHWN2_002711"
     CDS             <1..79
                     /locus_tag="ACHWN2_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932182.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHWN2_002712"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHWN2_002712"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR8932183.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="ACHWN2_002713"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="ACHWN2_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR8932184.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHWN2_002714"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHWN2_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR8932185.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHWN2_002715"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHWN2_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR8932186.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHWN2_002716"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHWN2_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR8932187.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="ACHWN2_002717"
     CDS             5377..5835
                     /locus_tag="ACHWN2_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR8932188.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="ACHWN2_002718"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="ACHWN2_002718"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR8932189.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="ACHWN2_002719"
     tRNA            6887..6971
                     /locus_tag="ACHWN2_002719"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="ACHWN2_002720"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="ACHWN2_002720"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHWN2_002721"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHWN2_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR8932190.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHWN2_002722"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHWN2_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR8932191.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHWN2_002723"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHWN2_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR8932192.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="ACHWN2_002724"
     CDS             complement(10252..13995)
                     /locus_tag="ACHWN2_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR8932193.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3541 a         3764 c         3423 g         3566 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcg
//
LOCUS       DAZSNO010000096        14260 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZSNO010000096 DAZSNO010000000
VERSION     DAZSNO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14260
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.096"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            163..1221
                     /gene="citC"
                     /locus_tag="ACHWN2_002725"
     CDS             163..1221
                     /gene="citC"
                     /locus_tag="ACHWN2_002725"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR8932194.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1236..1532
                     /gene="citD"
                     /locus_tag="ACHWN2_002726"
     CDS             1236..1532
                     /gene="citD"
                     /locus_tag="ACHWN2_002726"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR8932195.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1529..2437
                     /gene="citE"
                     /locus_tag="ACHWN2_002727"
     CDS             1529..2437
                     /gene="citE"
                     /locus_tag="ACHWN2_002727"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR8932196.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2448..3980
                     /gene="citF"
                     /locus_tag="ACHWN2_002728"
     CDS             2448..3980
                     /gene="citF"
                     /locus_tag="ACHWN2_002728"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR8932197.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3984..4534
                     /gene="citX"
                     /locus_tag="ACHWN2_002729"
                     /pseudo
     CDS             3984..4534
                     /gene="citX"
                     /locus_tag="ACHWN2_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4509..5387
                     /gene="citG"
                     /locus_tag="ACHWN2_002730"
     CDS             4509..5387
                     /gene="citG"
                     /locus_tag="ACHWN2_002730"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR8932198.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5438..6900
                     /gene="citT"
                     /locus_tag="ACHWN2_002731"
                     /pseudo
     CDS             5438..6900
                     /gene="citT"
                     /locus_tag="ACHWN2_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7014..7820
                     /gene="rna"
                     /locus_tag="ACHWN2_002732"
     CDS             7014..7820
                     /gene="rna"
                     /locus_tag="ACHWN2_002732"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR8932199.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8049..8459
                     /gene="rnk"
                     /locus_tag="ACHWN2_002733"
     CDS             8049..8459
                     /gene="rnk"
                     /locus_tag="ACHWN2_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR8932200.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8597..8686
                     /gene="yldA"
                     /locus_tag="ACHWN2_002734"
     CDS             8597..8686
                     /gene="yldA"
                     /locus_tag="ACHWN2_002734"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR8932201.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8690..9928)
                     /locus_tag="ACHWN2_002735"
     CDS             complement(8690..9928)
                     /locus_tag="ACHWN2_002735"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFR8932202.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10149..10577
                     /gene="uspG"
                     /locus_tag="ACHWN2_002736"
     CDS             10149..10577
                     /gene="uspG"
                     /locus_tag="ACHWN2_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR8932203.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10698..12263)
                     /gene="ahpF"
                     /locus_tag="ACHWN2_002737"
     CDS             complement(10698..12263)
                     /gene="ahpF"
                     /locus_tag="ACHWN2_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR8932204.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12508..13071)
                     /gene="ahpC"
                     /locus_tag="ACHWN2_002738"
     CDS             complement(12508..13071)
                     /gene="ahpC"
                     /locus_tag="ACHWN2_002738"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR8932205.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13443..14189
                     /gene="dsbG"
                     /locus_tag="ACHWN2_002739"
     CDS             13443..14189
                     /gene="dsbG"
                     /locus_tag="ACHWN2_002739"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR8932206.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3399 a         3776 c         3582 g         3503 t
ORIGIN      
        1 tcacgccgaa atactcctta ggatgtatag cgaaaggaga aaaagatata cctcgatcac
       61 ccccctttct cccaagtgaa aataaaaggt tatcagtttg caacattgaa caacattcgt
      121 tgcaaatcga taacaacatg caccttcagg atactattta ttatgttcgg caatgatatt
      181 ttcacccgcg taaaacgttc agaaaataaa aaaatggcgg aaatcgccca attcctgcat
      241 gaaaatgatt tgagcgttga caccacagtc gaagtattta ttaccgtaac ccgcgatgaa
      301 aagcttatcg cgtgcggtgg aattgccgga aatattatta aatgcgttgc tatcagtgaa
      361 tccgttcgcg gtgaaggact ggcgctgaca ttagccactg aactgataaa cctcgcctat
      421 gagcggcaca gcacgcatct gtttatttat accaaaaccg aatacgaggc gctgttccgc
      481 cagtgcggtt tttccacgct gacctgcgta cccggcgtga tggtgctgat ggaaaacagc
      541 gccacgcgac tgaaacgcta tgccgaatcg ctgaaaaaat ttcgtcatcc agggaacaag
      601 attggctgca ttgtgatgaa cgccaatccc tttacgaatg gtcaccgtta tctgattcaa
      661 caagctgcgg cacagtgcga ctggttgcat ctgtttttag tcaaagaaga ttcttcacgc
      721 ttcccctatg aagaccggct ggatctggtg ttaaaaggca ccgccgatat tccacgcctg
      781 actgtgcatc gcggctcaga atacatcatc tcccgcgcta cgttcccttg ctacttcatt
      841 aaagaacaga gcgtcattaa ccattgttac accgaaattg atctgaagat tttccgtcag
      901 tacctcgctc ccgcgcttgg tgtaactcac cgctttgtcg gtactgaacc cttttgtcgc
      961 gttaccgccc agtacaacca ggatatgcgc tactggctgg aaacgccgac tatctccgca
     1021 gcgcccatcg aactggttga aattgagcgg ctgcgttacc aggagatgcc gatatccgct
     1081 tcccgggtac gtcaactgct ggcgaaaaac gatctcacgg ctatcgcgcc gctggtccct
     1141 gcagtcacgc tgcattattt gcagaacctg cttgagcact cccgccagga cgcggcagct
     1201 cgtcaaaaga cccccgcatg agaaacaggt gaaaaatgaa aataaaccag cccgccgttg
     1261 caggcaccct tgagtctggg gatgtgatga tacgcatcgc cccactcgat acgcaggata
     1321 tcgacctgca aatcaatagc agcgttgaga aacagtttgg cgatgcaatt cgcaccacca
     1381 ttctggacgt tctcgcccgc tacaacgtgc gcggcgtaca gctgaatgtc gatgacaaag
     1441 gcgcactgga ctgcatttta catgcacgac tggaagccct gctggcacgc gccagcggta
     1501 tcccggctct gccatgggag gattgccaat gatttccgct tcgctgcaac aacgtaaaac
     1561 tcgcacccgc cgcagcatgt tgtttgtgcc tggtgccaat gccgcgatgg tcagcaactc
     1621 cttcatctac ccggctgatg ccctgatgtt tgacctcgaa gactccgtgg cattacgtga
     1681 aaaagacacc gcccgccgca tggtttatca cgcgctgcaa catccgctgt atcgcgatat
     1741 tgaaaccatt gtgcgtgtca acgcgctgga ttccgaatgg ggtgttaacg acctggaagc
     1801 cgtcgttcgc ggtggtgcgg acgttgtgcg tctgccgaaa accgataccg ctcaggatgt
     1861 tctggatatt gaaaaagaga tcctgcgtat agaaaaagcc tgtggtcgtg aacccggcag
     1921 caccggcctg ctggcggcga ttgaatctcc gctgggcatt acccgcgcag tggaaatcgc
     1981 tcacgcttcc gagcgtttga tcggtatcgc cctcggtgca gaagactatg tgcgcaacct
     2041 gcgtacagaa cgctccccgg aaggaactga actgctgttc gcacgctgtt ccattttgca
     2101 ggccgcgcgc tctgcgggta ttcaggcgtt cgataccgtc tattccgacg ctaacaacga
     2161 agccggattc ctgcaagaag ccgcccacat caaacagctg ggctttgacg gcaaatcgct
     2221 gatcaacccg cgtcagattg atctgctgca caacctctac gcaccgaccc agaaagaagt
     2281 ggatcacgcc caccgcgtcg tagaagccgc tgaagccgcc gctcgcgaag gcctcggcgt
     2341 ggtttccctg aacggcaaga tggtggacgg tccggttatc gatcgcgccc gtctggtgct
     2401 ctcccgtgca gaactttccg gcatccgcga agaataaggc aatcaaaatg acgcagaaaa
     2461 ttgaacaatc tcaacgacaa gaacgggtag cggcctggaa tcgtcgcact gaatgcgatc
     2521 ttgccgcttt ccagaactca ccaaagcaaa cctaccaggc tgaaaaagcg cgcgatcgca
     2581 aactgtgcgc caacctggaa gaagcgattc gtcgctcagg tttacaggac ggcatgacgg
     2641 tttccttcca tcacgctttc cgtggcggtg acctgaccgt caatatggtg atggacgtca
     2701 tcgcgaagat gggctttaaa aacctgaccc tggcgtccag ctccctgagt gattgccatg
     2761 cgccgctggt agaacacatt cgccagggcg tggttacccg catttatacc tctggcctac
     2821 gcggtccact ggcggaagag atctcccgtg gtctgctggc ggaaccggtg cagatccact
     2881 ctcacggcgg tcgtgtgcat ctggtacaga gcggcgaact gaatatcgac gtggctttcc
     2941 tcggcgtccc gtcctgtgat gaattcggta atgccaacgg ctacaccggt aaagcctgct
     3001 gcggctccct cggctatgca atggttgatg ccgacaacgc aaaacaggtc gtgatgctta
     3061 ccgaagaact gctgccttat ccgcataatc cggcaagcat tgagcaagat caggttgatt
     3121 tgatcgtcaa agttaatcgc gttggcgatg ctgcaaaaat cggcgctggt actacccgta
     3181 tgaccactaa cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg attgtcaact
     3241 ctggctactt caaagaaggt ttctccatgc aaaccggcac cggcggcgct tcgctggcgg
     3301 taacccgttt cctggaagac aaaatgcgta gccgcgatat tcgcgccgac ttcgccctcg
     3361 gcggtattac cgcgacgatg gttgatctgc acgaaaaagg tctgatccgc aaactgctgg
     3421 atgtgcagag ctttgacagc catgctgcgc aatcgctggc ccgtaacccc aatcacatcg
     3481 aaatcagcgc caaccagtac gctaactggg gttcgaaagg cgcatcggtt gatcgtctcg
     3541 acgtggtggt actgagcgcg ctggaaattg acaggcagtt caacgttaac gtgctgaccg
     3601 gttctgacgg cgtactgcgt ggcgcttccg gtggtcactg cgataccgcg attgcctctg
     3661 cgctttccat catcgtcgcg ccactggtac gcggtcgtat tccgactctg gtggataacg
     3721 tgctgacctg catcaccccg ggctccagtg tcgatattct ggtcacagac cacggtatcg
     3781 cagttaaccc ggcacgtccg gaactagcag aacgtctgca ggaagcgggc attaaagtgg
     3841 tttccattga gtggctgcgc gaacgtgcgc gtctgctgac cggtgaacca cagccgattg
     3901 aattcaccga ccgcgtcgtt gccgttgtgc gttaccgcga tggctcggtg atcgatgttg
     3961 tgcatcaggt gaaggaataa gccatgcacc tgcttcctga actcgccagc caccatgcgg
     4021 tgtcaattcc cgagctgctc gtcagccggg atgaaaggca agcacggcaa cacgcctggc
     4081 tcaagcacca tcctgttcca ctggtctcct ttaccgtggt tgcgcctggg ccgattaaag
     4141 acagcgaggt cacacgccga atttttaatc atcgcgtgac agccttgcgt gccttagcca
     4201 caaagcaggg ctggcaaatt caggagcagg ctgcactggt ttccgccagc gggccggagg
     4261 gcatgttgag cattgccgcc ccggctcgcg acctcaagct cgccaccatt gagcttgaac
     4321 atagtcatcc tctcgggcgg ttatgggata tcgatgtcct gacgcccgaa ggcgaaattc
     4381 tctcccgccg cgactattca ctgcgcctcg ccgctgcctg ttgtgcgagc aaagcgcagc
     4441 cgtctgcgcg cgtggaaaaa cccatcaact gaccgattta ctcaaccgca tggaggcact
     4501 gctgaacgat gtcgatgcct gcaacgtcaa ctaaaaccac aaagcttgcg acgtcattaa
     4561 tcgatgagta cgccctgctg ggctggcgcg ccatgctgac tgaagtcaat ctgtcaccga
     4621 aaccaggcct cgtggatcgc attaactgcg gtgcgcacaa agatatggcg ctggaagatt
     4681 tccaccgcag cgcgctggcg attcagggct ggttaccccg tttcattgaa tttggtgcct
     4741 gtagtgcaga aatggcaccg gaagcggtac tccacggatt acgcccaatt ggtatggctt
     4801 gcgaaggtga tatgttccgc gccactgcgg gcgtaaacac gcataaaggc agcatttttt
     4861 ctttagggct gctatgtgcg gcaattggcc gtttgcttca gctcaaccag ccggttacgc
     4921 caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg cctgaccgat cgcgaactgc
     4981 gtaccaataa ttcacgactg acggcaggtc aacggttgta ccagcagctt ggccttaccg
     5041 gcgcacgcgg tgaagccgaa gcaggttatc cactggtgat caaccacgcc ttgccgcatt
     5101 acctcactct gctggatcag gggttagatc ctgaactggc attgctcgat accttgctcc
     5161 tactgatggc taccaacggc gataccaacg ttgcatcgcg cggtggcgag gggggcctgc
     5221 gctggctaca gcgcgaggcg caaacattat tgcaaaaagg gggcattcga acccccaccg
     5281 atctcgatta tctccggcag ttcgacaggg agtgtatcga acgaaatctc agtccaggcg
     5341 gcagtgccga cctgctgatc cttacctgtt ttttagcaca gatttaatta tttaagcact
     5401 tgataaattt ggaaatatta attttcggag aacccgtatg tctttagcaa aagataatat
     5461 atggaaacta ttggccccac tggtggtgat gggtgtcatg tttcttatcc ctgtccccga
     5521 cggtatgccg ccgcaggcat ggcattactt cgctgtgttt gtggcaatga ttgtcggcat
     5581 gatcctcgag ccaattccgg caacagcgat cagttttatt gcggttacta tttgcgttat
     5641 tggcagtaat tacctgctct ttgatgccaa agaattagct gacccagcgt ttaatgcgca
     5701 aaaacaggcg ctgaaatggg gcctggctgg tttttccagc accacggtat ggctggtatt
     5761 tggcgcattt atttttgcat tagggtatga agtttccggg ttaggtcgtc gcattgccct
     5821 tttcctggtg aaattcatgg acaaacgcac gctgacgttg ggttatgcga ttgtcattat
     5881 cgacattctg ctggcaccgt ttacaccgtc caacaccgcg cgtaccgggg gtacggtttt
     5941 tccggtcatt aaaaacctgc cgccgttgtt taaatcattc ccgaacgatc cgtccgcgcg
     6001 tcgtattggc ggctatttga tgtggatgat ggtcattagt accagtctga gttcgtccat
     6061 gtttgtcacc ggtgcggacc aaacgtgctg ggtctggagt tcgtcagcaa aattgccggt
     6121 atccagatta gctggttgca gtggttcctc tgcttcctgc cggttggggt tatcttgctt
     6181 atcattgcgc cgtggctttc ctacgtgctg tacaaaccgg aaatcacaca cagtgaagaa
     6241 gtggcaacct gggcgggtga tgaactaaaa accatgggtg cgctgacacg cagagagtgg
     6301 acgctgattg gccttgtatt gctcagctta ggtttgtggg tatttggcag tgaagtcatt
     6361 aatgctactg cggttggtct gctggcagtt tcgctaatgc tggctctgca cgttgtgccg
     6421 tggaaagaca ttacccgcta taacagcgca tggaacacgc tggtcaacct ggcaactctg
     6481 gttgtgatgg ctaacggcct gactcgttct ggttttattg actggttcgc cggtaccatg
     6541 agtacgcacc tggaaggatt ctcaccaaac gcaacggtga ttgtactggt tctggtgttc
     6601 tactttgcac actacctgtt tgccagcctg tctgcgcaca ccgcaaccat gctgccggtt
     6661 attctggccg tcggtaaagg tattccgggc gtaccaatgg aacaactgtg tatcctgctg
     6721 gtgctgtcta tcggtatcat gggctgtctg acgccgtatg caaccggtcc tggggtgatt
     6781 atttacggct gtggctatgt gaaatcaaaa gattactggc gtcttggcgc aatcttcggg
     6841 gtgatttaca tctctatgtt gctgttggtt ggctggccga ttctcgccat gtggaactaa
     6901 tacgttcgcc aaacgctgaa taaatcatca cgcccgccag gtaagcccac ctggcgggct
     6961 tttttatgat ttaatagata gtattcagtg tatgtatgag ttccacaccc attatgaaag
     7021 cattctgccg taacgccgcg ttgctcgcgg tttctctgct tcccttatct tctgccaacg
     7081 ccgtagcgtt gcaggcaaaa cagtatggcg attttgatcg ctatgtcctg gccctctcct
     7141 ggcaaaccgg attttgccag agtcaacacg atcgaaatcg taacgaacga gatgaatgtc
     7201 gcctgcaaac cgaaacgacc aacaaaactg attttctgac cgtacatggt ctgtggccag
     7261 gattgcctaa atcggttgct gcccgtggtg ttgatgaacg ccgctggatg cgcttcggtt
     7321 gcgctactcg cccaatcccg aatctaccag aagcacgcgc cagccgaatg tgttcatcgc
     7381 cggaaaccgg gttatcactg gaaacggccg ctaaactaag tgaagtcatg ccaggagctg
     7441 gcggacgttc ctgcctggaa cgctacgaat atgccaaaca cggtgcctgc tttggttttg
     7501 atccggacgc atacttcggt acgatggtac gcctgaatca agaaattaaa gagagcgaag
     7561 ccggaaaatt ccttgcggat aattacggta aaacagtgag ccgccgtgac tttgacgccg
     7621 cctttgccaa aagctgggga aaagagaacg tgaaagcagt taagctaacg tgccagggta
     7681 accctgcgta tttgactgaa attcagatct cgatcaaagc tgacgccatc aacgctccgc
     7741 tttctgcaaa ctcattcttg ccacaacctc acccaggtaa ctgtggcaaa acctttgtga
     7801 ttgataaagc gggttattaa tcctctgatt tacaaaacac gctaattcgt cttccataac
     7861 catgccgatt aagaattata cataatgcgc tgcattgagg aattccctga atgtgacgca
     7921 aatcacttca aagagtgagc aacagtcagt atcatcatgt gagttaaacc ctcgccgcct
     7981 gacggtgagg gttttctttt gggtatgttt atctgcgaca ctcgcagtac cgacgacatg
     8041 gagtaaaaat gtccagacca actatcatca ttaacgacct ggatgccgaa cgcatcgata
     8101 ttctgctgga gcaacccgcc tatgctggtt tgccaatcgc cgacgcgtta aacgcagagt
     8161 tggatcgcgc ccaaatgtgt tcgccagaag agatgccaca cgacgtggtg acaatgaaca
     8221 gccgggttaa attccgcaat cttagcgatg gcgaagtgcg tgtgcgcacg ctggtgtatc
     8281 cggcaaaaat gaccgatagc aatactcagc tttccgttat ggctccggta ggtgccgcac
     8341 tgctggggct gcgcgttggc gattccattc actgggaact tccgggcggc gttgcaaccc
     8401 accttgaagt gctggaactc gaataccagc cagaagctgc tggcgactac ctgctttaat
     8461 cccatctgca caagcgataa ctcgctttgc ccagaggatg cactcgcatc ctctttacgc
     8521 aaaatcagca atttttcctg gttctcactg ttcacttttg ctgcaaaatt attccgtttc
     8581 gtttgtggga ataaccatgg cagaagcatt ttacatcctg ataggatttc tgattatggc
     8641 ggcgattatc gtcatggctg tcctttacct cgaaaaccac tcctgacgat catattgttc
     8701 cccccggcat cgcattcacc agacctgaaa ccgccttctg cgccgcctct gcgctttgtg
     8761 caccgggtac cagaatcacc ttacggcact cctcttcacg tttttcgaaa atctcatatc
     8821 cccgggcggc ctcttcaaac ggcatatagt gggtaacaat ttcttctggt ttcagtaatc
     8881 ctttctcaat taacggtaat aattctccca gccatgcgtg aacgtgggtc tgtcccattt
     8941 taaacgtcaa ccctttatca aaggcgtcgc caaacaggaa accgtgaata aatccagcgt
     9001 agacgcccgg tacgctaaca atgccgccac gcctgaccgc cgcaatacac tggcgcaacg
     9061 ctttaccgct gctgccctcc agtttcaggt tagtcagcac cgtttccgtg gtgctgcctt
     9121 tcgcttcaaa accgacggcg tctattactg catccacgcc ccggtgacct gccgtttgtt
     9181 caataattga ctgtgccgga tcgctatctt catcgaaatt aatcgggatc gcgccgtagc
     9241 ggtcagcggc gaaacgcaag cggtagggat gatgatcaac aacaaaaatc tgttccgcac
     9301 cgagcaaccg tgcacaggcg attgtcaaca atcccacagg accagcacca tagactgcaa
     9361 cgcttgaacc ttgttggatc tgcacatttt ttgctgcctg ccatgccgtt ggcagaatat
     9421 cagaaaggaa aagggcttta tcatctgaaa gcaaaggcgg tactttaaac ggccccacat
     9481 tccctttcgg gacgcggaca tattccgcct gcccgccagg aacgccacca tacaggtgac
     9541 tataaccaaa caatgccgcg ggagcaggta tctgtttttt attgagcgca gcgcctttac
     9601 ccgcattggt attttcgcag gcggcatatt gctgtaatcg acagaaaaaa cagtcgccac
     9661 aagcaatgac gaacggaatt accacccggt cgcctttttg caaatttttt acgtccttcc
     9721 cggtttcaac gacttccccc ataaattcat gaccaaaaat atcgccatgt ttaacctggg
     9781 gtattttgcc tcgataaaga tggaggtcag agccacagat cgccgttacc gtaatacgca
     9841 gaataatatc atctgcctgt tcaatgcccg gatcgggaac attttctacc tgaacgtgat
     9901 gtgggccgtg ataagtcaat gctttcatgc aacctccaga gacagttcaa aaaagagtca
     9961 gtctttaagg gtagctatca cagcgggaaa acgggcggta ccgcaggttt tgggagaatt
    10021 cctagtgttc agtgagaata tattaatcac agaaaccgtt cgtaaggaaa aagaaccaga
    10081 caaatatcag ggataaatat tataatcatg gagaatgatt tgttcatgat taacagggag
    10141 aaagggttat gtataaggca atcattatgc cagttgatgt atttgaaatg gaattgagcg
    10201 acaaagctgt tcgccacgct gaattcctcg cccaggatga cggagttatt catctacttc
    10261 acgtactacc cgggtcagcc agcctgagcc tgcaccgttt tgccgctgat gtgcgtcgtt
    10321 ttgaagagca tctgcaacat gaagcagaag aacgtctgca aacgatggtc agccacttca
    10381 ccatcgatcc ttcccgcatt aaacaacatg tccgttttgg tagcgtgcgg gatgaagtca
    10441 atgagttggc agaagaactg ggggctgatg ttgtagttat cggttctcgc aacccatcga
    10501 tttcgaccca tctgttaggt tctaacgcct cgagcgtgat ccgccacgcc aatctgcctg
    10561 tactggtcgt tcgctaacag ataaaagtat ttatgccgcc ctgcctggaa catcccggca
    10621 gggcttttta atgccataaa aaagccgcca ggtttgaccc cggcggctaa gcaattgcag
    10681 gtgaatctta cttcttctta tgcagttttg gtgcgaatca ggtagtcaaa agcactcaga
    10741 gaggctttgg caccttcgcc agtggcgatg atgatctgct tgtacggaac cgtcgtacag
    10801 tcacccgctg cgaacacgcc tttgacgttg gtttcacatt tcgcatcaat gataatctcg
    10861 cccatgcggt tacgttcgac tgcgccttcc agccagttgg tgttcggcag cagaccaatc
    10921 tggacaaaaa taccggccag ttcgatgttg tgaatattgc cgctgacacg atctcgatat
    10981 tccagaccaa cgactttgct gccgtcgcct ttcacttccg tggtttgcgc attcagaata
    11041 atgtcgacgt ttttcaggct gcgcagtttg tcctgcagaa cctggtcggc tttcatttct
    11101 ggcgcaaatt ccagcagcgt tacgtgctca acgatacccg ccaggtcgat tgccgcttcc
    11161 acgccggagt taccgccgcc gataaccgct acgcgtttgc ctttaaacag cgggccgtcg
    11221 cagtgcgggc agtaggtcac gcctttagtg cgatactgat cttcgcccgg aacgttcatg
    11281 ttgcgccatt ttgcacccgt cgccacgata atgctgcgtg ctttcagtac cgcgccagaa
    11341 gctgtttcaa tctggtgtag accaccttca accgctgccg ggatcagttt gctggcgctc
    11401 tggctgtcga tcacatcaac gtcgtattca tcaacgtgaa ctttcagtgc gcctgccaac
    11461 ttctgccctt cagtcttcgg tacagaaatg tagttttcga tatcaacggt atcgaggatc
    11521 tgaccaccaa aacgttcgcc catcagaccg gtacggatgc ctttacgtgc ggagtaaatt
    11581 gctgccgctg cacccgccgg gccggaacca acgattaata cgtcataagc atcacgcttg
    11641 ttcagctctt ctgccgcacg attttccgcg ccagtatcaa ttttggcaac gatttcagtc
    11701 aacgtcatgc ggccctgacc aaactctttc ccgtttacga acactgccgg aacgcccatc
    11761 acgttgcgat cggtgatttc gttctggaag gtgccgccgt caattgcagt gtgcttgatg
    11821 cgcgggttca atacgctcat caggttcagc gcctgcacca cgtccgggca gttatggcaa
    11881 gagagcgagt aataggtttc gaattcaaaa tcaccgtcaa tatggcgaat ctgctccagc
    11941 agagactgcg cttctttcga cggatgacca ccggtccaca gcaacgccag taccagcgag
    12001 gtgaactcgt ggcccagcgg ggagcctgca aaacgtggtc cctggttgga acctgggttg
    12061 gtgatcagga aagacggctt acgcactggc aagctgttat cttctttaaa ggtgactttg
    12121 tctgacagtt ctgcgatttc agccaacagt tccttgattt ctgccgattt agcgctgtca
    12181 tccagcgtgg caattaactc aacaggcttg gtcaatttct caaggtaagc cttgagttga
    12241 gttttcatat ttgtgtcgag catgtttatc tcctgggctt taaacatcat catgcaagcg
    12301 gcctctgcgg ctacctggat gcagcttgca tcatggtgcg gggaagaaaa tcgggtgccg
    12361 cagcacccga agaattaggt gaatttccga agtatttcgc gtcagagcaa ggcgactagc
    12421 ctgtgaatcc ccgggagctt acacaagtaa gtgaccgggg tgagcgggcg acgccaacgc
    12481 cgctatggcg tgaaagacga aggaaattta gattttacca accaggtcca gagacggagc
    12541 cagagttgct tcaccttctt tccatttagc cgggcaaact tcacctgggt gagaagctac
    12601 gtactgagct gctttgattt tacgcagcag atcagacgcg tcacggccaa tgccttcagc
    12661 ggtaacttcg attgcctgga tgataccctg cgggtcaaca acgaaggtcg cacggtcagc
    12721 cagaccttca tcttcacgca tgttgtcgaa gttacgggtc agggcgccag tcgggtcgcc
    12781 gatcatcgca tatttgattt tagcgatggt ttcagagctg ctgtgccatg ctttgtgggt
    12841 gaagtgagta tcggtagata ctgcgtatac gtctacgccc agtttctgca gttcttcgta
    12901 gtggtcagca acgtcaccca gttcggtcgg gcatacgaaa gtaaagtcag ccgggtagaa
    12961 gaagaagacg ctccagcggc cttcggtatc tttttcggtg atttcgatga attcgccgtt
    13021 tttgaatgcc tggtttttaa aaggtttaat tttggtgtta atcaaggaca tctatacttc
    13081 ctccgtgttt tcgatgagat gtaaggtaac caatttttgc cgattcggct aatgcgtttc
    13141 cattatcaaa tcgataagtt ttaccttaca accttcgtaa gacaactttg tgaactttgc
    13201 accgccagcg gcgataaaaa gtaaaatggc cccctttcca ggtgaccgtg taatctgaac
    13261 taccctttgg tatcttcagc gcctgcctgc aggctgaggt gttgccctta caaatgcaac
    13321 aacgacatgg attacaacac cctcataaac aaagggcaat cacctgatct aagctcttac
    13381 ctatgacagt gataggttat gccttttact cgacttttgc actgactgaa aagaacaaat
    13441 taatgttaaa aaagatactt ttactggctc tgcttcctgc aatcgccttc gcagaggaac
    13501 ttccttcccc agtaaaagcg attgaaaaac agggcattac aatcatcaaa acgttcgatg
    13561 cccccggagg aatgaaaggt tatctcggaa agtatcagga tatgggcgtc accatctacc
    13621 tgactcccga tggtaagcac gctatctctg gttacatgta caacgagaaa ggtgaaaacc
    13681 tgagtaacac acttatcgaa aaagaaattt acgcaccagc cggacgcgaa atgtggcaac
    13741 ggatggaaca atcccactgg ctcctcgacg gtaaaaaaga tgcgccggtc attgtctacg
    13801 tcttctccga tccgttctgc ccatattgta aacagttctg gcagcaggcg cgcccgtggg
    13861 tagattctgg caaagtgcaa ttaagaacat tgttggttgg ggttatcaag ccagaaagcc
    13921 cggcgacagc agcggcaatt cttgcctcca aagatcccgc aaaaacctgg caacaatatg
    13981 aagcctctgg ttgcaagctt aagctaaacg tgcctgcaaa cgtaagtaca gagcaaatga
    14041 aagtgttaag tgacaatgag aaactgatgg acgatctggg ggcaaatgtc acgccggcta
    14101 tctattacat gagtaaggaa aatacgctgc aacaggccgt ggggttgccc gatcagaaaa
    14161 cgcttaatat cattatggga aataaataaa aggtaattca tggatggttg aactatatcc
    14221 ctgcttttca accatcctaa aaaagcatga atttctcgct
//
LOCUS       DAZSNO010000097        13651 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZSNO010000097 DAZSNO010000000
VERSION     DAZSNO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13651
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.097"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..406)
                     /gene="yhaJ"
                     /locus_tag="ACHWN2_002740"
     CDS             complement(<1..406)
                     /gene="yhaJ"
                     /locus_tag="ACHWN2_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFR8932207.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLA"
     gene            511..1212
                     /locus_tag="ACHWN2_002741"
     CDS             511..1212
                     /locus_tag="ACHWN2_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR8932208.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1235..1399
                     /gene="yhaL"
                     /locus_tag="ACHWN2_002742"
     CDS             1235..1399
                     /gene="yhaL"
                     /locus_tag="ACHWN2_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR8932209.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1611..2921)
                     /gene="cyuA"
                     /locus_tag="ACHWN2_002743"
     CDS             complement(1611..2921)
                     /gene="cyuA"
                     /locus_tag="ACHWN2_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR8932210.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2949..4280)
                     /locus_tag="ACHWN2_002744"
     CDS             complement(2949..4280)
                     /locus_tag="ACHWN2_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR8932211.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(4555..5919)
                     /gene="tdcG"
                     /locus_tag="ACHWN2_002745"
     CDS             complement(4555..5919)
                     /gene="tdcG"
                     /locus_tag="ACHWN2_002745"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR8932212.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5991..6380)
                     /locus_tag="ACHWN2_002746"
     CDS             complement(5991..6380)
                     /locus_tag="ACHWN2_002746"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR8932213.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6394..8688)
                     /gene="tdcE"
                     /locus_tag="ACHWN2_002747"
     CDS             complement(6394..8688)
                     /gene="tdcE"
                     /locus_tag="ACHWN2_002747"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR8932214.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8722..9930)
                     /gene="tdcD"
                     /locus_tag="ACHWN2_002748"
     CDS             complement(8722..9930)
                     /gene="tdcD"
                     /locus_tag="ACHWN2_002748"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR8932215.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9956..11287)
                     /gene="tdcC"
                     /locus_tag="ACHWN2_002749"
     CDS             complement(9956..11287)
                     /gene="tdcC"
                     /locus_tag="ACHWN2_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR8932216.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11309..12298)
                     /gene="tdcB"
                     /locus_tag="ACHWN2_002750"
     CDS             complement(11309..12298)
                     /gene="tdcB"
                     /locus_tag="ACHWN2_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR8932217.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12397..13335)
                     /gene="tdcA"
                     /locus_tag="ACHWN2_002751"
     CDS             complement(12397..13335)
                     /gene="tdcA"
                     /locus_tag="ACHWN2_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR8932218.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3614 a         3642 c         3167 g         3228 t
ORIGIN      
        1 ccgccagcac ttctgtgatg attgccagtt gggtattggc ttttgccgcc agtttgtcga
       61 ttaacgggaa aaaggcaggt gtcggtacca gcgcttcggt cacaatggtg agatgcgttt
      121 cccaaccgcg cgcgagagct tccgcatcag tagtcagttt atctgcggct tccagcaaaa
      181 cgcgccctcg ctccagcaac atcctcccga cattggtgaa tttggtgcga tggcccgagc
      241 ggtcaaacag caccacatcc agctcttctt ccagtttttg catggtgtag ctaagtgcgg
      301 aaggcacgcg tcccagctca tccgctgccg ccgcaaaact gccccggcga tcgatcgcat
      361 ccataacccg tagtgcttcc agcgttaatg ccctttcttt ggccatttcg ttctcattca
      421 ggaaatttga acataccggg cagaatatct ggctaacaat gtagcgtcca gccccttacc
      481 ataaaaggaa gtaaagagag gtcaagaatt atgattacta cccgaactgc caggcagtgt
      541 ggacaagcag actacggatg gttgcaggcc cggtatactt tttcctttgg acactacttc
      601 gacccgaaat tgttaggcta tgcctccctg cgtgtgctta accaggaagt gctggcccca
      661 ggtgccgcct ttcagccgcg aacctatccc aaagtcgata ttttaaatgt gattctggat
      721 ggagaagcag agtatcgcga cagcgaaggc aatcatgttc aggccagcgc cggagaggcg
      781 ttgctgctct ctacccagcc gggtgtcagc tatagcgaac acaatctcag caaagacaaa
      841 ccgttaacgc gaatgcagct ttggctggat acctgcccgc agcgagagaa tccgctgatt
      901 caaaagctgg cgcttaatat ggacaagcag caattaatcg cctcgccaga gggtgcgatg
      961 ggaagcctgc aattacgcca acaagtgtgg ctgcaccata tcgtgctcga caaaggcgaa
     1021 agtgcgaatt tccagttgca tgggccacgc gcgtatttgc aatcgattca cgggaaattt
     1081 catgcgctta cgcatcatga agagaaagcg gcgctaacct gcggtgatgg ggcgtttatt
     1141 cgtgacgagg ctaacattac gctggttgcc gattccccac tgcgcgcttt gctgatagat
     1201 ttgcctgtct agttgttttt acagggagat gatgatgagt aaaaaatcga ccaaaaagcg
     1261 ccagccagcg aagcccgtgg tggcgaaaga acctgctcgc accgccaaaa attttggcta
     1321 tgaagagatg ttgagcgagc tggaagctat cgtcgcggat gctgaaacgc gtttagccga
     1381 ggatgaagct accgcgtaaa gacaggttgc cggatgcggc gtaaacgcct tatccggcct
     1441 acaaaacaca acaacatcaa caaattaaac accccgtagg ccggataaga tgcgccagca
     1501 tcgcatccgg catcaggtac aaaacacaac aacatcaaca agttgagcac cccgtaggcc
     1561 ggataaggtg cgccagcatc gcatccggca caatactcaa aacctggcgt ttatctggcc
     1621 ttgctcgcca taatctcgat aatctgccga tccgtttgct gcatcgaatg gcttgctaac
     1681 gcacacaggt tggcaatcga ctgctcaaca tcatgcgcca cgatcccttc attgccggtc
     1741 acggcggtat catccagcgc cattaacacc gctttccacg cagccgaagc actggtcgaa
     1801 accttcatcg cgcagctgtt cgacgcacca tcgcaaatca tgccgctgac atcgccgatc
     1861 atactgctga tcgccatcga gatagtttca taacgcccat ccaccagcca tgccatcccg
     1921 gcggcggccc ccattgctgc ggtcgttgcg gcacaaagtg cagacaaacg cggtaactgg
     1981 ttatggatgt aaattgcgct caaatgagaa agcatcagcg cacgcgccag tcgttcatca
     2041 tccgctccga agtgttctgc tacaaccacc acaggcatta ttgcagtgat cccctggtta
     2101 cccgagccgg agttactcat agccggaagc gtagcgccgc ccatacgcgc atcggatgcc
     2161 gcgctggtac gaatcacaat ggatgaagag agatctttcg ccagcaaacc gcgctcgcac
     2221 tgtttttcca gcgtcgcgcc aatatgcagc ccccacttac cgctcaaacc ttcctgcgat
     2281 aacgcacaat ttagcttcgc ggaatcgaga ataaagcgga tcgccgcaaa tgggacttca
     2341 ttgacgaact tcaggatctc agccagcgtc gttctggaaa gcaccgtcag cggcgactct
     2401 tgctcgccct ctgccacaca cgcctggtgg gtaaacacca caccattgtg cgtctcaata
     2461 tgcacaatgt tggtatgccc gccgacgatg gtgacacacg cccacttctc accgttccag
     2521 actttggcgc gtgagaagag gatttcattg caaggttcct ggatcttaac agagactttc
     2581 cccgccgcca gcagtgcttt ggcatcggta attgcctgcg cagttgcgtc tttcagcact
     2641 tccagcccgg cgttggcatt tccacctaac gcccccagcg ccgccgcaat cggcagcccc
     2701 accattcccg tgccgggaac ggtgacgccc agaccgttct tcatcagatt tggcgaaacc
     2761 caggcttcta cacgttcaac cggaccttcc agttctgccg cagcaaccgc cgccgccagc
     2821 gccagtgaaa tcggttcagt acatcccagc gccggtttta cttcctcctg aacggcgagg
     2881 atgtaacgct gccataacgg atttaaagtc gaatcaaaca tattaaaaac cttaaaattt
     2941 caggtaaatc aagaaaatgc caggaacaga gaaacacaaa gcaacaaacc agtgacgata
     3001 atcaggtaca gagacatccc tttgtatttg tgcaatgccg gtactttgta aaccagccac
     3061 gccgggatca ggcaccctac catgccgaaa atcgggctac agatagaggt gaagctcaac
     3121 accggtgcgt tcagtacgat ggcgctccag gccagcaaaa tggcgaaaat catgatgccg
     3181 cgctgaacaa gattttcgtt aatcttctcg gcaggcatct tgcgacgcag gatgttcatt
     3241 acgatccctt gcgttgcttc gcgaaagcct aaatagacgc caaagaatgc agtcatgaca
     3301 gcaaagatat tgagaatgac gctgaccact ttcacccacg ctgcgccgtc accgctaata
     3361 aactgtgcgg caatcgccag cgcggaaata ttctgctcgt aggcttttac cgcttcgtca
     3421 tgtcccatcg ccagcgtgaa cgacacggcg tagaaaaaga cggtgacaaa caaaatgcca
     3481 aacgcgatat tcattgcccg caatgcttta tgccgcgcca cttcaattga tttttcccgc
     3541 gagcgataag agatcaccat cgggcttaac gtctggataa acagaatcga cgtcagggta
     3601 aacggcagcg taataatggc gtttttcacc agcagcccca gcggcggtag cgaaccgacg
     3661 ttatacagat gccacattcc caccatcgac acgcccagcg cagcgactac cagcagcttg
     3721 gtcagcacca tgtcggtcga aattttgaat aacaatttct cgccgcgtga agagatcgcc
     3781 accagaatgc aaatcagtac cagaccataa aagggactgt ctgacagcaa cccttccgtc
     3841 acgccgaagg tatgcagata ggaagcacta tcgttggtga tggcggtgga atagacgaac
     3901 atccaaatca ccagcattac gaaatagagc gcacctaaca ggatgcccca gtttttacct
     3961 aaataaccgc taatgacact cgggtaatct ttacattctg gtgattctgc cagcgtatta
     4021 ataaacaacc gctgaaacag atacattgcc gggtaaccaa tcaccgatga gagcaaaaat
     4081 acccacaatc ccatcaaacc gacctgcacc gggagaaaaa caatccccgc gccaatcgcc
     4141 atcccgatac tcataatcac ccagccggtg tcagtactgt cgaatttgat cgcttctcgc
     4201 cactcgctct cactcattcc agcacgaccc gccggggtcg aagcgtctgc aatgacgcct
     4261 ttattcgatg caatttccat aatttctcgc tcaatatttt gtagggctta ttattttttt
     4321 ccgagccgca tcaaggcgat atgcggtcta cgtttttgca ggcgagttat tagaataaag
     4381 aaatgatacg cgcgaggtca gagaaaatct tcacaatcaa gactttaaaa taacggagat
     4441 ggataagaat tttctactaa attaatcaca agagaatatt cattctctat ttgcggcaga
     4501 tcacaaaaaa aaggtgcaca tttgtgcacc caaggatgaa agctgacagc aatgtcagcc
     4561 gcagaccact ttaatggcca gtcctccgcg tgatgtttcg cggtatttat cgttcatatc
     4621 tttgccggtt tcatacatcg tctcgatcac tttatcgagt gaaacacgcg gtgccgaggt
     4681 gcggcgcatc gccatccgcg cggcgtttac tgctttcacg gcattaatgg cattacgttc
     4741 aatgcacggg atttgtacct gtccggcaac cggatcgcag gtcagcccaa ggttatgctc
     4801 catcgcgatt tccgccgcat tgcatacctg cgccggactg ccgcccagta gttcagttaa
     4861 ccctgccgct gccattgaac aggccacgcc aatctccccc tgacagccga cctccgcgcc
     4921 agagatggag gcgttcattt tatacagcgc gccaatagcc cccgctgcca gaaaatagcg
     4981 ggcaattgac cgctcgttta ccggacgacg gaacttatcg taataagcca gcactgccgg
     5041 aataatgccg cacgcaccgt tagtcggtgc cgttaccacg cgcccgccag ctgcgttttc
     5101 ttcactaacc gccagcgcgt acatgttgat ccagtcgatg acattcatcg gatcgttaga
     5161 gatgttatcg ctagaaacca gctgacgacg cagcgctacg gcacgacacg gcacattgag
     5221 cggaccaggc agcacgcctt cagtgttcat cccgcgttca ataccgtcat gcatcacttg
     5281 ccagatacgg gcaaaccctg cgtcaatttc cgctttgctg cgcagcgcca gctcgttgtg
     5341 catcatcagg ccagatatag acaggccgtt gtaatcacac attttcagca gttcacctgc
     5401 tgagtggaaa tcgtaaggta cggacgtttc gacatcgtgc gacaggccga agtgttcttc
     5461 ttcgacaata aacccgccgc cgacagagta ataggtttta cttaatagcg cttcctgtcc
     5521 cttccaggca gtgatccgca ttccgttctc atggcgaggc aacatttcgg gatggaagat
     5581 aatgttcttt gctacaggaa aatcaacaat atgcgcacca gatgccactg gcagccgccc
     5641 gctgcgcgtt actaactcta taaatgcagg gatctcatca atgacaacat cctgcggact
     5701 gtttcctgcc agtcccatga taatggcgac atccgtggca tggcctttgc ccgtcagtga
     5761 caacgacccg tacagatcga ccacaatatg gctcgtcgcg gttaataagc cgctactttc
     5821 cagccgatca ataaaacttt ttccggcatt cattggcccc acggtatgcg aactggaggg
     5881 accaatccca attttgaaaa tatcgaatgc actaatcata tccacaccct cggattgccg
     5941 ttcagtgaag tggagcggaa cgaccttgcg accgtcccgc tcacgaggct ttacgcacta
     6001 cgtactgcga tagcttcaat ttccagcttc acatctttcg gcaaacgcgc aacctgcaca
     6061 cagctccggg tcgggtaggt cgcctgatgc tcatcgaaga actgcttata gacttcattg
     6121 atggtggcaa aatcattcag atcggtgatg aacacggtca tcttgatgat atcgcccacg
     6181 ctcagcccgg cagcaaccac gatcgctttg acgttttcga ggcttaaacg cgcctgatct
     6241 tgcacatcag ccgggatctc accggtctgc gggcaaaccg gaatttgccc ggaggtgaag
     6301 accatgctgc ctaaatcaac gccctgaaca taagggccga ttgcgcctgg ggcacgttgc
     6361 gtttcgataa tctttttcat acatcctccg gcgtcagagc gcctgggtaa aagtacgtga
     6421 aataacatcc tgttgctgtt cacgggtcag tgcgttgaag cgcacggcgt agccagagac
     6481 acggattgtc aggttaggat atttttccgg gtgctcgatg gcatccagca gcatttcccg
     6541 attcattacg ttgacgttga ggtgttgacc gccttcgaca tccgcttcgt ggtggaaata
     6601 cccatccagc aggccgacaa ggttggtttt acgtactgga tcttctttgc ccagcgccgc
     6661 aggaacgatt gagaaggtgt acgagatccc atctttggcg taggtgaacg gcagtttcgc
     6721 caccgacgtc aatgaggcca cggcaccttt gcggtcacga ccatgcatcg ggttagcgcc
     6781 cggcgcgaac ggtgttccgg cgcgacgacc gtccggcgta ttaccggttt tctggccgta
     6841 caccacgtta gaagtgatag tcagaatcga ctgggtaggg acggcgttgc gataggttgg
     6901 cagcgcttta attttcttca taaagcgttc aaccaagtcg caggcaatgc tgtctacgcg
     6961 ctcgtcgttg ttgccgtact gcggatattc accgtcgatt tcaaagtcca ccgccaggcc
     7021 gttttcgtca cggattggtt tcacgcgggc atatttgatg gcagacaggg agtccgtcgc
     7081 caccgacagg cccgcgatgc cgcatgccat agtgcgatag acatcacgat cgtgcagcgc
     7141 catcagcgaa gcttcgtagc tgtacttgtc gtgcatgtag tgaatgatat tcagcgcgct
     7201 gatgtactgc accgccagcc agtccatgaa gtgatcgagg ctgtccatca ctttgtcgta
     7261 atccagcacg tcgtccatca gcggtgctgt tttcggcccg acctgaatct tcagcttctc
     7321 gtccaccccg ccgttaattg cgtagagcag cgttttcgcc aggttagcgc gtgcaccaaa
     7381 gaactgcatt tgcttaccaa tcaccattgg gctgacgcag caggcaatcg cgtaatcgtc
     7441 gctgttgaag tcagtacgca tcagatcgtc attttcatac tgcaaggaag aggtgacgat
     7501 cgacacctgc gcggcatatt ttttgaaggc aatcggtaat tcttccgacc aaagaatggt
     7561 caggttaggt tccggtgccg gccccatagt gtgcagggtg tgcaaatagc ggaaggagtt
     7621 tttggtcacc agcgtacgac cgtccagccc catcccgccg atcacttccg tcgcccagat
     7681 tgggtcgccg gagaacagcg aatcaaattc cggtgtacgc aggaagcgta ccatacggat
     7741 cttcatgatg aagtgatcga tcagttcctg tgcctgctgc tcattgagga cgccagcttt
     7801 aaagtcgcgc tcaatgtaga tatccaggaa cgatgccgtg cgccccagcg acatcgcgcc
     7861 gccattttgc gatttcactg ccgccagata agcgaagtag agccactgca ccgcttcctg
     7921 tgcattctgc gccgggcgag agatatcaaa gccatatttc gccgccattt cctgaatctg
     7981 caacagcgca tgacgatgct ctgccagctc ctcacgcaga cggatggtgg cttccagatc
     8041 ctcgcctttt tccagacgag actggagatc ggcaaattgc agttcgcgtt cacgcaccag
     8101 ataactgatg ccatacagcg ctacgcggcg atagtcaccg ataatgcgcc cacgaccata
     8161 gccatctggt aaaccggtca gcacgccaga tttacggcag cgcagcatat ccggtgagta
     8221 aacatcaaat acgccctggt tatgggtttt acgcagatcg gtaaacagat attcaaattc
     8281 actgtccatt tcacggccat aggcgtggaa tgaactttta atcatattaa tgccaccgaa
     8341 cgggtgtagc gcacgtttca acggcgcatc cgtttgcagg ccaacaattt tttccagcgg
     8401 ctggttaata tatcccgcat catgagcggt aattgtggtg gcaatattgg tatcgaaatc
     8461 aaccggcgcg tgggttgcgt tttcgatacg gatgccttcc attacctttt cccacaattc
     8521 cgtggtggca ggcgtcgctt cggcgagaaa agattcatcg ccttcatacg gtgtatagtt
     8581 atgttgaata aaatcgcgga cattaatttc gcttttccag tccgtacctt taaagccaag
     8641 ccatgcgtca gcgtacagct tatcgctggt atcaatatct accttcatga aaaataatct
     8701 ctctacaata cttcaactaa attatgcaaa ttctgcgggc gcgttaactt tacctaaatg
     8761 aatggcatcc aaagcaatca ttttttcttc gttagtcgga ataacgacgc aaatgacatg
     8821 cgcattttca ctggaaacaa ttcgctcgcc aaaagagtta gaacgattat tcatttctgt
     8881 atcaatctct acgcctaata cagccaaatg ttccatgacc agacgacgaa ttaaacttga
     8941 attctctcct attccgccgg tgaatataat tccatccagc cgacgtaatg aagctgcatg
     9001 tccggcaata tgacgggcaa ttcggtgaac aaaggtttta attgccagtt gcgcgcgttc
     9061 gtgaccttca tgccaggctt tttccagaac acgtaaatcc gaagaaagac cggaaatacc
     9121 taataatccc gactctttat tcactacgcg ttccaggtca cccaggctct ggttggtttg
     9181 gctggcgacc caggacatcg caccaaagtc gacatcgcca ctgcgggtac ccatcatcaa
     9241 gccttccagc ggcgtcattc ccattgaggt gtcaacactc tgaccgttgc gaactgcgca
     9301 gattgacgcg ccattgccaa gatgcgccac aaccaggccg gagtcatctt ccgccagatt
     9361 cagcagcgaa tgtgcgcgct gggaaacata gcggtgcgac gtgccgtgga aaccataacg
     9421 gcgtacacct aactcttcat aatatttcca cggcaggccg tataaataag cttccggagc
     9481 catcgtctgg tggaaactgg tatcaaatac cgccacctga gttacgcccg gaaataattg
     9541 ctgcgccgat tcaataccac ttaaatttgc gtaattatgc aggggtgcca gtggagaaac
     9601 gcgacggata ttatcaatga cttcatcggt aataatggcg gactcggtaa aaatactgcc
     9661 gccgtgagcg atgcggtggc caattaaggc cacgctgtca tttaaattcc gtttttccag
     9721 ttcaaatgca attgccttca atgcaccttc gtagctgtgg tgagccagcg gtgctggctc
     9781 tcccccattt acggataaga atgcattttc cgagttaata ccgtcggcaa tacctgacat
     9841 taatacttca caatcgctgg catcgagtac ggaaaactta atcgaagacg aaccacagtt
     9901 aataaccaaa acaaccggaa attcattcat ctcttttctc atcctgagtt acggattaaa
     9961 acagtttgta tacgatgttc aggatggtca gcagaccaat cacggtaaca aacacgttat
    10021 ccagacgacc acggtatttc gccagagacg gcgctttacg gatagcatac atcggcaaca
    10081 ggcacagcag ggatgcgata atcggtgcgc ccatggcttc aatcaggtcg aggatgttcg
    10141 ggttggcgta ggcaacaacc caggtggagc ccatgatgaa gatcatgctg agagtattca
    10201 gtttacccag cgacactttg gttttgtcac ctttataacc gaacttcaga atcagaccat
    10261 tcaagccttc cagcgtcccc agatagtgac cgaagaaaga tttgaagata gccacgagtg
    10321 cgatgatgga agccgcatat tccagtgtaa tcgcgaacgt tgttttcgta ccggtcatgg
    10381 acgcaaagtg gttagccaga taagaaagca ctggaatatt ctgcgctttg gcttccgcca
    10441 tgttggccgg agacagagta aacaggcagc taaaggcaaa gaacatcacc actgcaacca
    10501 tcagcatgct ggcacgagaa atgatttggg aacatttacg ttcggtgaag tcgcgaccga
    10561 agtctttctc atactcttca cgtttagaaa ccacgaagga agagacgatt ggcgagaagt
    10621 taaaggagaa aaccatgatg gaaatcccca gccagacagt gatcaggata ccgtcatgac
    10681 cggttaacga cagcgaaccg aggtcaacct ggtcgataac tgcagagttc cagtaaggga
    10741 tcagcgacag agaaatcagc accaggctgg cgataaacgg ccataccagg tagctcatca
    10801 ctttaaccat cagatcctta ccaaaccaga tgacgaaagc catcagcagc aacaggaaca
    10861 gcgccacaaa gccgcgattc agcggtgcaa atccgagctg gttttcccag aacgtcataa
    10921 aggtattggt aatagtaacg ccataaatcc acagcagtgg gcaaatcgcg aagaagtaca
    10981 ggaacgtgat aaccacgccg ccagttttac caaaatgctc ttccaccgtt tccgtaatgt
    11041 tgccggaagg gttagagcca gaaagacaca gacgcgccag cgcccggtgg caataaaatg
    11101 cgatggggta tgccaatacc aacatcagaa gaatcgggat cagtccgcca aaacctgcgc
    11161 ggatagggaa gaacagcacc ccggcgccga ttgccgtacc aaacaagcct aacgtccatg
    11221 tggtatctga tttacgccag gacgattgtt ttgtctggct ggatacaatg ctatctgaag
    11281 tactcatatc ctatcctcaa cgaattaatt aagcgtcaac gaaaccggtg atttgagaga
    11341 cgcgagaaag atcgatattg ccgccggaaa taatactgac ggtttttcta ttttgaatat
    11401 attggtctaa tttaccgctt aataatgcag cacatgccag agcgcctgcg ccttcggtga
    11461 cgactttatt gcgctgaatt aaggcaatca tactgtttct gatttcgtct tcgctgacca
    11521 gcacgatgtc atcgactaat tcacgaacga tttcgtaagt taaattaccc gggcgggaga
    11581 catcacaacc atccgccagg gtgccggtag ttcggtgcgt ggttatttct ccggagtgga
    11641 aagaagccgc catgccgtga acgttttctg actgtacgcc aataacacga atggtcgggt
    11701 taatagattt aattgccacc gcaataccag caattaaacc accaccacca attggcacaa
    11761 tcacgttatc gacatcatag agatcttcca taatttccag accaatcgtt ccctggccag
    11821 caatcacttt cggatcatcg taaggtggga taaaaatacg accttccatt tcgacaattt
    11881 cgctcacttt agcgatagtg tcgttgaagt tatcaccatg cagaacgact tctgcggagt
    11941 agtcgcacgt tgccgctact ttggattttg gcgcaccttt tggcatcacc actttaccgt
    12001 cgatacccag catcgcgcag gagagcgaaa ccccttgcgc atggttgccc gcagaacagg
    12061 ccaccacgcc tttgcgtttt tccgcatcgg tcagtgaact taatttatta aatgcgccac
    12121 gaattttaaa tgaacccgta cgctgcatat tttcaaactt caggaatatt tcacctttgc
    12181 aacgttcact aaaatagttg gagcgaggca tgcctgtttt ataaattcgc ccagccagtc
    12241 gttgtttcgc ttcaataata tcatcaatag caaccggcag atcgtatgta atatgcatta
    12301 taaaacctct tcgcctgaat taaatatgta ggtaaccgta accgacacct gcaaaacaga
    12361 caggtggatt atttatgttt aaataaaaac aaacaactaa ccaacttcta ttaattgcct
    12421 tcgtctacac ccattataag atgaatactc tttggctaat tccaccaaaa ccgatgctgc
    12481 ttttttaata cgataatttt tcgaccatac cgcggcatat tgtgccacag gtaatgtttc
    12541 ttcaaccgga atagtaataa attgattaga accaaaaggt gacgtcatat cacaaggaat
    12601 tacagttaag aaatcagcat tgagaacaag attataaatt gtcacgactg agtcggtttt
    12661 aacgatgttt tcaatactga tgccatttct ttgtaacgta gtaagcagtt cgctgtagta
    12721 ccccatatta gtttgtggca acacccactg ttcgttcttc aacgactcca gcgtggtggt
    12781 gccggtgcat gttcgggact tactggctac cagcacaaac tcggactcga acagcggctc
    12841 aacatgtaaa tcctgaagct tcatttctgc acttaacgta ccaatcgcaa aatccagtcg
    12901 accgtcgcgg attgccggta agaacgaaga cagttgcgct tcatacatag aaacctgcgc
    12961 tttcgggaac acctctttga acttgttgat catccctgac ataaaagtaa aaccaatcaa
    13021 tgaaggaaaa ccaaatgaga cttccaccac cgcttcagaa gacataccgc ttatctcatt
    13081 aaccatattt ttcatttcac gggtaatgga ttcggaacgg gagagtaaca attgaccggc
    13141 aggtgttaat gttacaccgg tatttttccg caccactaat tccacaccaa aataatcttc
    13201 aatatcgtta atgattttac tgacggccgg ttgagttaac cctaattctt ttgcagccga
    13261 gccgatagag ccacttctaa tgacttcctg aaagactacc aggtgctgcg ttttcggaag
    13321 aagaatagtg ctcataacga cctacgttaa ttacctcatt gacggcatga agtgtatcaa
    13381 aatgaaataa acaggatatg tgcgaccact cacaaattaa ctttcattac tttccaaggt
    13441 atcgttatta caattaaaca tctttaaata tcaacaagtt aaagcataaa aatcagcata
    13501 aaccctgatt tttatcaaaa aaaaatgacg gggataacca tattttatgg cgataacatc
    13561 attcgttatg tggttaatac aaaaaagggg ctgagaccat atttcaagca attaccggat
    13621 aattaccagg caaaatttga gttaaattta a
//
LOCUS       DAZSNO010000098        13148 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZSNO010000098 DAZSNO010000000
VERSION     DAZSNO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.098"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            105..1604
                     /gene="pitB"
                     /locus_tag="ACHWN2_002752"
     CDS             105..1604
                     /gene="pitB"
                     /locus_tag="ACHWN2_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR8932219.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1653..2345)
                     /locus_tag="ACHWN2_002753"
     CDS             complement(1653..2345)
                     /locus_tag="ACHWN2_002753"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR8932220.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2519..3232
                     /locus_tag="ACHWN2_002754"
     CDS             2519..3232
                     /locus_tag="ACHWN2_002754"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR8932221.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3264..4022
                     /locus_tag="ACHWN2_002755"
     CDS             3264..4022
                     /locus_tag="ACHWN2_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932222.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            4068..5417
                     /locus_tag="ACHWN2_002756"
     CDS             4068..5417
                     /locus_tag="ACHWN2_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR8932223.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5417..6241
                     /locus_tag="ACHWN2_002757"
     CDS             5417..6241
                     /locus_tag="ACHWN2_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932224.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            6253..6813
                     /locus_tag="ACHWN2_002758"
     CDS             6253..6813
                     /locus_tag="ACHWN2_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR8932225.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6844..7914
                     /gene="lptF"
                     /locus_tag="ACHWN2_002759"
     CDS             6844..7914
                     /gene="lptF"
                     /locus_tag="ACHWN2_002759"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR8932226.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            7911..8990
                     /gene="lptG"
                     /locus_tag="ACHWN2_002760"
     CDS             7911..8990
                     /gene="lptG"
                     /locus_tag="ACHWN2_002760"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR8932227.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9026..10197)
                     /locus_tag="ACHWN2_002761"
                     /pseudo
     CDS             complement(9026..10197)
                     /locus_tag="ACHWN2_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(10197..10445)
                     /locus_tag="ACHWN2_002762"
     CDS             complement(10197..10445)
                     /locus_tag="ACHWN2_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR8932228.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10478..11392)
                     /locus_tag="ACHWN2_002763"
     CDS             complement(10478..11392)
                     /locus_tag="ACHWN2_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR8932229.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11389..13077)
                     /locus_tag="ACHWN2_002764"
     CDS             complement(11389..13077)
                     /locus_tag="ACHWN2_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFR8932230.1"
                     /translation="MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         3150 a         3232 c         3420 g         3346 t
ORIGIN      
        1 taacactctt tttatcgtta aaaaatgata tttcactttg cccatgccgt taaagtcact
       61 gataatgcgt ccgttcgtaa attcaaaatg gcgtaatcta atatatgcta aatttatttg
      121 ttggccttga tatatatacg gggcttttgt tattacttgc tctggcattt gtgttgttct
      181 acgaagcaat caatggtttt catgacacgg cgaatgcggt ggcaaccgtt atttatactc
      241 gtgccatgca gccacaactt gctgtggtga tggcggcatt ttttaacttt tttggcgtgt
      301 tattgggcgg acttagcgtt gcctatgcca ttgtccatat gttaccaatc gatttgttgc
      361 tgaatatggg gtcaactcac ggtttggcga tggtcttttc catgctgctg gcggcgatta
      421 tctggaacct gggaacgtgg ttctttggtt taccggcctc cagttcgcac accttgattg
      481 gtgcgattat cggcatcggt ttaaccaacg cgctgttaac cggctcatcg gtgatggatg
      541 cgttaaacct gcgtgaagta accaaaattt tctcctcgct gattgtttcc cctatcgtcg
      601 gcctggtcat tgcgggtggt ctgattttcc tgctgcgccg tttctggagt gggacgaaaa
      661 agcgtgaccg tattcaccgc attccggaag atcgcaaaaa gaaaaagggc aaacgtaaac
      721 cgccattctg gacgcgtatt gcgctgattg tttccgctgc gggcgtggcg ttttcgcacg
      781 gcgcgaacga cgggcagaaa gggatcggcc tggtaatgct ggtactggtg gggattgccc
      841 ctgctggctt cgtcgtcaat atgaatgcgt ccggctatga aattacccgt acccgcgatg
      901 ccgttaccaa cttcgaacac tacctgcaac agcatcctga actgccgcag aagttgattg
      961 cgatggaacc tccattgcct gcagcatcga ctgatggcac gcaagtaaca gagtttcact
     1021 gtcatccggc aaataccttt gatgcgattg cgcgcgttaa aacgatgctg ccaggcaata
     1081 tggaaagtta cgagccgtta agcgtgagtc agcgcagcca gctgcgccgc attatgctgt
     1141 gcatctccga tacctccgcg aagctggcga aactgccagg cgtcagtaaa gaagaccaga
     1201 acctgctgaa aaaactgcgc agcgatatgt taagcaccat tgagtacgct ccggtgtgga
     1261 tcatcatggc ggtagcactg gcgctcggca ttggcaccat gattggctgg cgtcgtgtag
     1321 cgatgaccat cggtgagaag attggtaagc gcggcatgac gtatgcgcaa ggcatggcgg
     1381 cacaaatgac ggcggcagtg tctatcggcc ttgccagtta tattgggatg cctgtctcca
     1441 caacacacgt cctctcgtct gcggtagcag ggacgatggt ggtggacggc ggtgggttac
     1501 agcgtaaaac ggtaaccagc atcctgatgg cgtgggtgtt tactttaccg gcggcaattt
     1561 ttctttctgg tgggctgtac tggatagcat tgcagttgat ttaaccttcc tgaaaatgcc
     1621 cggtcctggc gatcgggcat ttccattttt gactagtgat aaccacgcgc ggtcataaaa
     1681 tccgtaatcg ctttttctgc atcaaccaac acctgttcca gtggctgatt ggcatcgata
     1741 tcaaccagtt gtgcaccacc aaaggttaac tgtggcgtta tggcaatctt cctcgccagc
     1801 gattcccgtt tatggtcggg tttacgtgca caggcaactt caaggtcaac attgagtttg
     1861 atgaccagat caggcttatg gctcgccatc cagtgaaacg ctttacgttc ctggcttgcc
     1921 agccatgaga caaaacgacc accttcaacg ttaggtggga acaccgtacc atcgtaagcg
     1981 ccaggaattt ggtcctgagg ataacggtcg gttagaacaa ttaacccgcg acgacgacag
     2041 gcaagcatat gacgaaagcg cagtaagcga cgggcgacaa acgctgtaat taccagcgcc
     2101 ggaactggtc caggcaattt ttttgctgtt ttcacctgat ttcgttcaat tgttttatgt
     2161 aaggattttc ccatcaaagg taattttgtc actgcacgac cgacatttcc ggcctgtttt
     2221 cctaaatgaa ctctttcggc agcaccatat ttttcgacaa cggtaataag atgttcacac
     2281 accgttgact tgcctgaagc atcgctacca ataacggcaa ttaatggagg tgttattgac
     2341 tgcatgaata tatcctttaa tttaaatatc cattaaaaat atttatttgg ttaatatgtt
     2401 tttatgaaag cgtaattcag gtcaatgtca caattagtca ttgtcacaat aaggttgaac
     2461 ggatattctg agcacggacc acgtaatatt caatacatta tttactgccg ggtttttcgt
     2521 gaatcaacgt aatatgtcaa taattaattc cacgccagtg cgtgttattg ccattgtagg
     2581 atgtgatggt tcaggtaaat cgaccctcac ggcaagcctg gtaaatgaac tggcagcaag
     2641 aatgccaaca gaacacattt atctcgggca atcgtccggg cgaattggcg aatggatttc
     2701 acagctccct gttattggcg caccttgtgg gtgttatctg cgaagtaaag cggcacatgt
     2761 acacgaaaag cccttaactc cgcctggcaa tattactgcg ctggttatct atctgctttc
     2821 ctgctggcgg gcgtacaagt ttcgcaaaat gttgtgtaaa agccagcaag gctatctgct
     2881 catcaccgac cgctacccgc aggttgaagt gccgggtttt cgctttgatg gcccgcaatt
     2941 ggcaaaaacc acgggcggta acggttggat aaaaatgtta aggcagcgcg agttgaagct
     3001 gtaccaatgg atggcgtctt atttacccgt attgttgatt cgtcttggca ttgatgaaca
     3061 aaccgcgttt gcgcgtaaac ctgaccacca actggcagcg ttacaggaaa aaatcgccgt
     3121 tacgccgcaa ctgacattca atggcgccaa gatccttgaa ctggatggtc ggcaaccagc
     3181 cgatgaaatt atgcaagcgt cactacgcgc aattcacgcc gcgctttcct gatcaccaaa
     3241 atggataggg gatgatttaa cgaatggatg cactacaaac acaaactgtt aatagtacaa
     3301 ccgcgccgca gcccaactac attccggggc tgattgcggt ggtcgggtgt gatggcaccg
     3361 gtaaatccac gctgaccacc gacctggtga aatcgctgca acaacactgg caaaccgagc
     3421 gccgctatct ggggctgctc tccggcgaag acggcgacaa aatcaaacga ttgccgttgg
     3481 ttggcgtctg gctggaacgg cgattggcgg ccaaatcctc gaaaacccaa agcatgaaaa
     3541 ccaaatctcc ggcgctatgg gcggcggtga ttatgtactg cttctcgctg cgaagaatgg
     3601 cgaatctacg caaggttcag cgactggcgc aaagtggcgt tctggtggtc agcgatcgct
     3661 tcccgcaggc tgaaatttcg ggcttttatt atgacggacc ggggatcggc gtcgaacgtg
     3721 cgaccgggaa aatcagcatg tttctggcgc agcgcgaacg gagtttatac caaaaaatgg
     3781 cgcaataccg tcctgaatta atcattcgtt tggggattga tattgatact gccatctccc
     3841 gcaagcctga ccatgagtat gccgagctgc aggacaaaat cggcgtcatg tcgacgattg
     3901 gctataacgg cacaaaaatt cttgagattg attccagagc gccctacagc gaagtgctgg
     3961 aacaggtaca gaaggcggtt tctctggttg ccatcgtttc tgaccgccga agtttaactt
     4021 aaggctggcg ggatattttt tactatcaac gataaggtca ggctgaattg gcgggtttta
     4081 acatcaaaca ttggtttgca gatggcgcgt ttcgcaccat tattcgcaat agcgcctggt
     4141 taggctccag taatgtcgag agcgccttgc tggggctgtt ggcgctctcg tgtgccggta
     4201 aagggatgac gcccgccatg tttggtgtac tggtgattgt gcaatcgtac gccaagtcga
     4261 tcagcgattt tattaagttt cagacatggc agctggtggt tcagtacgga acgccagcct
     4321 tgaccaacaa taatccgcag caatttcgca atgtcgtctc attttccttc tcgctggata
     4381 tcgtcagcgg cgcggtggcg attgtcggcg gcattgcctt actgccattc ctttcccatt
     4441 cattaggtct ggatgaccaa agcttttggc tggcggcgct gtattgcacg ctcattcctt
     4501 caatggcttc ctccacgccg accggcattc tgcgtgcggt agatcgcttt gatttgattg
     4561 ctgtacagca ggcgacgaaa ccttttctgc gcgcagcggg gagcgtcgca gcctggtatt
     4621 ttgactttgg ttttgtgggt tttgttattg cctggtacgt gtcgaatctg gttggcggca
     4681 ccatgtactg gtggtttgcc gcgcgcgaat tacgccgtag aaatatccat aacgccttca
     4741 aattgaatct gtttgagtct gcccgacaca ttaaaggcgc gtggagtttt gtctggtcaa
     4801 ccaacattgc ccactccatc tggtcggcgc gtaactcgtg cagcacagtg ttggtgggga
     4861 tcgtgttagg acccgctgcc gccgggttat ttaaaatcgc catgacattc ttcgacgccg
     4921 ccggaacgcc agcgggtttg ctgggtaaaa gtttttaccc agaggtgatg cgtttagatc
     4981 cgcgcaccac cagaccgtgg ttgttaggtg tgaagtccgg tttactggcg ggcggaatcg
     5041 gtattctggt ggcgctcgcg gtgttgatcg tcggcaagcc gctcatttcg ctggtgtttg
     5101 gcgttaagta tctcgaagcg tatgacctga ttcaggtgat gctgggcgca attgtgattt
     5161 cgatgctggg cttcccgcag gagtcattgc tattgatggc gggaaaacag cgcgcttttc
     5221 tcgtggcgca aaccatcgcc tcaatcggct acatcgtatt gctgttcatg ttctgtcatc
     5281 tgtttggtgt gctgggagca gcgtttgctt acttcggtgg gcaatgtctg gacgtggcgc
     5341 tctcgttaat tccaacctta aaggcatttt ttcagcgcca ttccttgctt tataacgcag
     5401 ccggagagaa atcctgatga tgagaaaata tttcccgctt gaggcgagcg agcgtctatt
     5461 tgttgcgatt gaagaagatg atgtcgttga tgcacaggtt agtttgccgc cgactattgc
     5521 actaagttgt accaccgaaa tcattcatga taattacgcg ttatgcctgc aattctggct
     5581 caatggcgtt gatcgtcagg aattactgcg tttagttcgc aaacaagcca aaggcgatga
     5641 gctgactgct gatgagcgca aacaatttaa atacatgcga gcgcgctaca agcacctgcg
     5701 ttttgcgcaa cggctgtatt taaagaagca tcaggccgga tttttattcg gcaaaacgac
     5761 cgtttttctg gggcgttttc aggacggctt tcgtaacggt aagaaaaata ttgtgtcgta
     5821 ttacggcaac ctgttacgga tttatttaag ctctccggtc tggtcgttgg tgaactattc
     5881 attacgccat agccagttag aaagcgtaag cagttttatt gcctatcggc aaaagcaaat
     5941 gcataccctg aaagagatta ttgccaaacc gcgactaacc ggcagggaat ttcatgatgt
     6001 gcgaaaaatc atcagccagc aggtttctta ttacgacacc ttaagatcgc ttgatcctga
     6061 aaataaagag gcgttacaga tttcccgttt cctcgccgcc attaatggcc tgatgggaga
     6121 taaacacgac gacatggtgg cggatgatat ggaaaaccgc cagtcgtatt atgcaccgtt
     6181 ggcgctggat agcgatattt gtcagcgctt agagctgctg atttcgcgtt ttccgctata
     6241 gccggagcaa tgatgtcatc tcgtttgatt attgcgttga ttatcatgct cttagccccg
     6301 ggtgtgcagg cacacaattt cgtcactggc aaaacggtta cccctgttta tatccaggaa
     6361 ggcggcgagc tgcttctgaa tagcgacgac gaaattcact accaaaagtg gaacagcaca
     6421 caattagcgg gaaaagtccg cattattcag tatatcgccg ggcgtaagtc ggcgaagaaa
     6481 aagaactcac tgttaattaa agcggttgaa gcggcaaact ttccgcagga tcgttttcag
     6541 cccaccacca ttgtgaacac cgatgatgcc atctttggta ctggctattt tgttgttggc
     6601 aaaatcgaaa aaaataaacg ccgctatccg tgggcgcaat tcgttattga cggcaatgga
     6661 cagggacgcg ttgcctggcg cttgccggag cagagttcaa ccattctggt gctgaataaa
     6721 gccgggcaaa ttcagtgggc aaaggacggc agcctgacgc cggaagaggt agatcatgtc
     6781 attgcgttgg cgcagaaact gatcaatgaa taaaaaactc aaaggattga gggcgttagt
     6841 gagatgaagc tggttgaaca ctacatcatg cgtggtacgc gccgtctggt gctgattatc
     6901 gtcggttttc tgatctttat tttcgccagc tactccgcac agcgttatct gaccgaagcg
     6961 gcaaatggga cattagctct tgatgttgtg ctggatatcg ttttctacaa agtactgatt
     7021 gcactggaga tgttgttacc tgttggtctg tatgtgtcag ttggcgtgac gctagggcag
     7081 atgtataccg actcggaaat taccgctatc tctgcggcgg ggggcagtcc gggacgcttg
     7141 tacaaagccg ttctttatct ggcgataccg ctaagtattt ttgtcaccct tctgtcgatg
     7201 tatggtcgtc cgtgggctta tgcgcagatt tatcaactgg agcagcagtc gcagtcggag
     7261 ctggatgttc gccagttgcg ggcaaagaaa tttaacacta acgataacgg acgaatgatc
     7321 ctttcgcaga cggttgatca ggataataat cgcctgactg acgcgctgat ttatacttct
     7381 actgccaatc gaacccgtat tttccgcgcc ggttcggttg atgtggttga cccatcaccc
     7441 gagaaaccga ccgttatgtt gcacaacggc actgcctatc ttctcgatca tcaggggcgt
     7501 gacgacaacg aacagatcta ccgtaatctg caattacatc tgaatccgct ggatcaaagc
     7561 ccgaacgtca aacgcaaagc gaaatcggtc acggagctgg cgcgctccgc ctttcctgcc
     7621 gatcatgccg aactgcaatg gcgacaaagc cgtggcctga ccgcattgtt gatggcgctg
     7681 ctggccattt cattaagtcg ggtaaaaccg cggcaagggc gattttcaac gttattgcca
     7741 ctgacgttgc tgtttattgc cattttttat ggcggcgacg tctgccgtac actggtggct
     7801 aacggcgcga ttcccctcat tcctggtttg tggttagtgc ccggactcat gctaatgggc
     7861 ctgctgatac tggtcgcacg tgacttctct ttgctgcaga aattttcccg atgaatgttt
     7921 tcagtcgcta tttaatccgt catctctttc tcggttttgc cgccgctgca gggctattgc
     7981 tgccgctttt taccaccttc aacctgatta acgaactgga tggtgtcagc ccgggcggtt
     8041 atcgctggac tcaggcggtg ctggtggtgc taatgacctt accgcgcaca ctggtcgaac
     8101 tttcgccgtt tatcgcgtta ttgggtggta ttgttggact ggggcagtta tcgaaaaaca
     8161 gtgagcttac cgccattcgc agcacggggt tttctatctt ccgtattgca ctggtggcgc
     8221 tggttgccgg gatattgtgg actgtttcgt taggcgcgat agatgagtgg gtggcgtcgc
     8281 cattgcagca acaggcgttg caaatcaaat cgaccgccac cgcgttgggg gaggacgatg
     8341 acattaccgg caatatgctg tgggccaggc gtggtaatga atttgtgacg gtgaaatcgc
     8401 tgaacgagca gggccagccg gtgggcgtgg agatatttca ttatcgcgac gatctctcgc
     8461 ttgaatccta catttatgca cgcagtgcca ccattgaaga cgacaaaacg tgggtcctgc
     8521 atggtgtgaa tcataaaaaa tggctgaatg gcaaagaaac gctggaaaca ttagataatc
     8581 ttgcctggca atcggccttc accagtatga atcttgaaga gttatcaatg ccggggaata
     8641 ctttttctgt ccgtcagctt aatcattaca tccattattt gcaggaaacc gggcaaccca
     8701 gcagcgaata ccaccttgca ctgtgggaaa aactggggca acccatcctg accctggcga
     8761 tgattttgct ggctgtgccg tttaccttta gtgccccgcg ttcgcctggg atgggtagcc
     8821 gtctcgctgt aggcgtcatc gtgggcttac tcacctggat cagctatcaa atcatggtta
     8881 atctgggatt gctatttgcg ttaagcgcac ctgttaccgc gctcggttta ccggtagcgt
     8941 ttgtgttggt ggcgttgagc ctggtgtatt ggtatgacag acaacattaa acccgccgtg
     9001 gcgcaaacac cacggcgggc tcacattact gcttcagagt agcaaatgcc tgaatgatgg
     9061 catcgatttg ctcacgactg tgggcagcat ttacgctaca gcgcagcagc gtgatgcctg
     9121 caggcgcggc aggcggcagg ataagattca catacacgcc cggcgagata agctcgcgcc
     9181 agatgcgtaa accttcctct ttcgaaccga ttattactgg cactaccggg ctaatatgtg
     9241 aacttaactc ataacccagt ttttgcagac cgtcatacag atggttggcg ttatccatca
     9301 gcttttgccg cagctctggt tgtgtggcaa tagttcttaa cgacgagcgc acggtcgcaa
     9361 tgcaggacgg agaaggagag gcggtaaaaa tatacgggcg gctgccgtag cgcagcactt
     9421 ccatcgcttc cgaaacgaca gcaaaaccgc caatcgaggc cagactttta ctgaaagtac
     9481 caacgataat atccacatca tcttcaacgc cgacagcttc agccagtccg cgccccgttg
     9541 cgcctaacac gccaaacgaa tgcgcttcat cgacaatcaa ataaccaccg agacgacgtt
     9601 tgatatcaac aatttccgcc agcggcgcaa cgtcaccgag catgctgtag atgccctcaa
     9661 cgatgataat cgcctctttc gcccgttcac cgaggcgaac catgcgccgt tccagatctt
     9721 tagcatcgtt atggcggaaa cgaataattt ccgcgccacc gagagaacag acgtcataaa
     9781 tactggcgtg gctgtcggca tccagtaaca ccacggcgtt atggtcggca agtgcgctga
     9841 tgacgcctaa attagcggta taaccggttg aaaaacgata gcggtagggc gattgaaaaa
     9901 ctcggcaatc tctttctcta acgccaggtg cggcgcataa ctgccgtttg ccatacgaga
     9961 accggtggtg ccggtgcctt gggccgctaa cgcggcctga ccttcgctga tggcatcatg
    10021 attaaaggtt aaaccgagat aattattggt cccggccaga atcacttttt tattgccgat
    10081 ccggccttcg gtggcggaat acacttcatc aatacaggtg ccaaacggtg ttaacccgtt
    10141 gtcactaaat tgcagacgct cgccagcaag gcgcgcatat ttatcgtata gccccattat
    10201 ttattctcca gccatggaag tagagtttcc attaattgcg caggggtttt gacatccagc
    10261 agaatattaa taggaatgga aatatcgaat ctgtcttcca gcatcattaa gagatccatc
    10321 actttgatcg actccaggcc aagatcgttg acgagatcgc tgtcgggctt aatttccaca
    10381 ccgttttcga ccaaatcctg taaacaggaa aggatgtaat ccattactat ttcacgattt
    10441 accatagaaa cgttaacaca cctttaaaac aagacaatca aaatagcccg ttgcgtaatg
    10501 cgtgttccag gctaatcccg ggaaaccaat tgatatcttc agaaatacgg ttattacttg
    10561 ccgaccagtc ggcgtgggtt aattcacgaa ttttcgagcg ggtcagcata ggttctttac
    10621 ctgccaggcg actcaacgcg gtactgatat ccgcaaggcc tgtgagtacc ggcagaggaa
    10681 tgtcaaccat tcgcacggaa ccacaacggg ctttggcggc aagctgtcgt acgcgttgcc
    10741 agtcatagcc accggcgacg ccatcgcata attcataggt ttgcgtctgt acagtttcgg
    10801 cgcttaacca ctgacccact gcttgcgcaa aatcggtgac gtgaagaaaa gagagttgtg
    10861 tatctggcgc accaagtcgt ggcagcaggc cgcgtagcat ccagtcaaac agcggtttta
    10921 actctttatc gccgggacca tacacggctg tcgggcgaaa aacgcccagc gtaatttcgt
    10981 ccgccattgc tgccagtcgt tgctcggcga cgtatttgga ttttgcatac caggagagct
    11041 cgggatggcg cgccgccagc gaagagataa acagaaaacg ttggcaaaag ccgctctctt
    11101 ttgctgcttg catcaggcgc aggctgccgt caacgttaca gcgggtgaaa atctcttctt
    11161 tgtgcccgcg cacttgcccg gcgcaatgga ccactacgct ggctccggca accagctcgc
    11221 taagtgaatg agtatcttcc agcgaaccgc gcacccaggt gagattatca ttgacgtgag
    11281 cgcgggcagt acgcgtcaat gcgcgaacgt gaaagccgcg ggcgagcagg ttatcgataa
    11341 tatatttacc gataaacccg gtagcgcccg tcaccgcgac agtttgattt atgccaggga
    11401 ttcctgcaca ttaagactgg cagcataagc cttctgataa cgttttttcg cttccgcacg
    11461 ggcaggcttg ccggaggacg ttcggggaat actgtggggc ggcaacagat cgatagccgc
    11521 ggtcacgcca aattcgcttt ggatccgtgc cgccagcgcg tggataagct gcccgcgacg
    11581 ttcttcgtcg ctgatccgac actggatctg caaaatgatt ttttcctggg cggtaacaaa
    11641 agcaatcgca tcgccagaat gaatttccgg ttcctgttcc gctatatatt caatatcctg
    11701 cggccagata ttacggccac gaataataat cagatcttta atgcgtccgg tgacatacag
    11761 ataaccgtcc agcagataac cgaggtcgcc ggtgtctaac cagcccgtcg cggcaatctc
    11821 gtcttgcgaa acctggtcgc caaagtaacc gctcatcaga ctgggaccgg agatgcaaat
    11881 atggcctacc acacgttccg cgaccggcat acctgcttca ttgcggattt caataccatg
    11941 ttccggcaac gctttgccgc agttgacgaa agtcgatacg gcgcgtgtct ctgcacccgg
    12001 cgcgacggct ttaccctgat attcgaggat gtcgcgatcc acttcgttaa ccacaacccc
    12061 ggaggcttca tcagagaagc tgacagccag cgcattttct gccagtccgt agcacggcat
    12121 gaaagtttta ttgtcaaagt taacctgacg gaaacattca gcgaattgat ggagttgttc
    12181 tgcggagatc ggctcagcac caataccagc gacgcgccag caggaaagat ccagttcagc
    12241 gagatctttt tcattcacgc ggcgttggca caattcatag ccaaacggcg gcgcaacgga
    12301 aacggtgccg cgatttttac tgatcaattt aagccattgc aaaggacgca tggcaaaatc
    12361 ctgagtgcgc aaataatcta ctgaaagttg cgtggcgacg gggggcagga gaaagccgac
    12421 cagtcccata tcatggtaga aaggctgcca ggagacgcag cggtcgctag ggcgtaattt
    12481 aatgccatcg tggcttatag cacgtagatt agccatcact tcgcgatggg tgataatgac
    12541 gccacgggga aaacgggtgc tgccggaggt gtactggagg taggcgatat cgttcggaac
    12601 tggacgctgg agcgcaacat cggcttccgg taatgcctta aaccaagcgt ggcttaaaac
    12661 atgtaattcg gggttgttat gcgtcgcggc attgaccagt ggcaaccact catcaccagt
    12721 gataatggct gcgggctggc aacttgccag taaaccctgg agtttggcgc tccaggaatc
    12781 ccgctgaccg acgcccattg gaatcgccaa cgggacggcg actaagccag catactgaca
    12841 ggcaaaaaaa gcctctacaa acccgctact cgtttcggca atcagcgcca cgcgttcgcc
    12901 ttttttcagg ttcagcgata acaaccgctt cgcaccagct tcggcacgcg tttttaatgt
    12961 ctgatattcc agttgatctt caagttggca acgtctgtca taaaaattca ttccggctct
    13021 actcagagcg gcgtagtcca aagcatcaac cagcgttgga aaatcggcat agcgcatagg
    13081 tagggaatgc gtaaagattt tattagacat atacaccacg aaaaaattgg atatcaaaaa
    13141 taaaatga
//
LOCUS       DAZSNO010000099        13104 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZSNO010000099 DAZSNO010000000
VERSION     DAZSNO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13104
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.099"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..896)
                     /gene="yafC"
                     /locus_tag="ACHWN2_002765"
     CDS             complement(<1..896)
                     /gene="yafC"
                     /locus_tag="ACHWN2_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR8932231.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGG"
     gene            1137..1937
                     /locus_tag="ACHWN2_002766"
     CDS             1137..1937
                     /locus_tag="ACHWN2_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR8932232.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2015..2785
                     /locus_tag="ACHWN2_002767"
     CDS             2015..2785
                     /locus_tag="ACHWN2_002767"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8932233.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2833..4179)
                     /gene="mltD"
                     /locus_tag="ACHWN2_002768"
     CDS             complement(2833..4179)
                     /gene="mltD"
                     /locus_tag="ACHWN2_002768"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR8932234.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4251..5006)
                     /gene="gloB"
                     /locus_tag="ACHWN2_002769"
     CDS             complement(4251..5006)
                     /gene="gloB"
                     /locus_tag="ACHWN2_002769"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR8932235.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5040..5762
                     /locus_tag="ACHWN2_002770"
     CDS             5040..5762
                     /locus_tag="ACHWN2_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8932236.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5759..6226)
                     /gene="rnhA"
                     /locus_tag="ACHWN2_002771"
     CDS             complement(5759..6226)
                     /gene="rnhA"
                     /locus_tag="ACHWN2_002771"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR8932237.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6291..7022
                     /gene="dnaQ"
                     /locus_tag="ACHWN2_002772"
     CDS             6291..7022
                     /gene="dnaQ"
                     /locus_tag="ACHWN2_002772"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR8932238.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7155..7231
                     /locus_tag="ACHWN2_002773"
     tRNA            7155..7231
                     /locus_tag="ACHWN2_002773"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7189..7191,aa:Asp,seq:gtc)
     gene            complement(7362..8408)
                     /locus_tag="ACHWN2_002774"
     CDS             complement(7362..8408)
                     /locus_tag="ACHWN2_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFR8932239.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8422..8981)
                     /gene="tssG"
                     /locus_tag="ACHWN2_002775"
                     /pseudo
     CDS             complement(8422..8981)
                     /gene="tssG"
                     /locus_tag="ACHWN2_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8978..10861)
                     /gene="tssF"
                     /locus_tag="ACHWN2_002776"
     CDS             complement(8978..10861)
                     /gene="tssF"
                     /locus_tag="ACHWN2_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR8932240.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10877..11371)
                     /gene="tssE"
                     /locus_tag="ACHWN2_002777"
     CDS             complement(10877..11371)
                     /gene="tssE"
                     /locus_tag="ACHWN2_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR8932241.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11368..12198)
                     /locus_tag="ACHWN2_002778"
                     /pseudo
     CDS             complement(11368..12198)
                     /locus_tag="ACHWN2_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(12185..13099)
                     /gene="tagK"
                     /locus_tag="ACHWN2_002779"
                     /pseudo
     CDS             complement(12185..13099)
                     /gene="tagK"
                     /locus_tag="ACHWN2_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3397 c         3485 g         3215 t
ORIGIN      
        1 cctccgggag ctgtttttac atgctcgcta aggaaatcga taaaagcccg gatgcgcgta
       61 cttaccgcac ggtcgctgta atagacggca ctgaatggca tttccactgg caacacttta
      121 tctgccatta actccaccaa ttctccgcga gcgatttctt tgtcgatcat gtagtcggac
      181 aaacacgcaa tcccgttgcc actcaggcaa agctgtttca gtgtttcccc actattggat
      241 gacaaaccgt acttcacctc atgtaattgt ccatcactac aggctatcgg ccaggtattg
      301 agggaagcgg gttcagtgaa tcccaggcaa acatgttgct ttaaatcgtc gatcgtttct
      361 ggcttcccgt agcgggaaat ataatcgggg gaggcgataa tttttcgata actgttaaat
      421 aacggcctgg cacgtaagct ggagtccgtt aacgtaccag cgcgtatcgc gacatccact
      481 tttctttcga tcaaattaat aatcgtttcg gaggagacta gcgataaagt gacttccgga
      541 tagcgttcac ggaaaggctt aattaacggc atcagaaagt gcagcaccac tggagttgcg
      601 gcatcgatcc gtaacagtcc acgcggtgta ttacgcgtct ccataatttc tgattctgcc
      661 gctgccatct cctgcaaaat tgactgtacg cgacgaaaat aacgctcgcc ttcttccgtc
      721 aggctaagtt gccgcgtggt ccgattaagc aggctaacgc caagtttcat ttccagcttt
      781 ttcaccgccc ggcttaccgc tgagtttgct tgccctaatt gttccgctgc ccggctaaag
      841 ctgccgcttt cgacgaccga aacaaaaatg gcgagttctt ccgacgtggc tttcattttt
      901 gctcctgttg caaaatagat gagatatttt gaatttattt gtcattaaac catcaggatg
      961 cgtgatatgt catccgattt aatgttctca ataatgagca aatttctgac aggtgtaagc
     1021 acttgcttac ataacaatat acaattgctc gttgaaagag tgagctaaaa tccctataac
     1081 agtagaaccc tcccgagtgc ggaagggttg acgtaataga ggtttcaaag tcaaaagtgc
     1141 gaaaaaacac ctatgccatg cgctatgttg ccggacaacc tgcggaaagg atcttaccgc
     1201 cggggtcttt tgcgagcatc ggccaggcat taccacctgg agaaccgtta agtaccgaag
     1261 agcgtattcg gatcctggtg tggaacatat acaaacagca acgcgctgaa tggttgtcgg
     1321 tattaaagaa ctacggcaaa gatgcacatc tggtgttatt gcaggaagcg cagacaacgc
     1381 cagagttagt acagtttgcg accgctaact atcttgccgc cgatcaggtg cccgctttcg
     1441 tgctgccaca acatccttcc ggtgtaatga ccctttcggc ggcgcatcca gtgtattgct
     1501 gcccgttacg tgaacgagaa cccattttgc gtctggcgaa gtcggcactg gtgacggtct
     1561 atccattacc tgacacccgc ctgttgatgg tggttaatat acacgccgtc aacttcagtc
     1621 tgggcgtgga tgtctatagt aagcagttac ttcctattgg tgatcagata gctcaccaca
     1681 gcggcccggt cattatggcg ggagatttca atgcctggag ccgtagaagg atgaacgcgt
     1741 tatatcgctt tgcgcgggaa atgtcgctgc gccaggtgcg ttttaccgat gatcagcgcc
     1801 gtcgggcgtt tggccgcccg ctcgattttg ttttctaccg tggtctgaac gtcagcgaag
     1861 cttctgtatt ggttacgcgc gcttccgatc acaatccgct actcgttgaa ttcagtcccg
     1921 gcaagcctga taaataaggt atgtcaggtc tgccacaggg cagaccaacg tttggcgctg
     1981 tccttttata ctcaaacaac aaaaggacag cgcaatgaca acacaatccc accatgacca
     2041 cgtagaaaag cagtttagct ctcaagcctg cgaatatctg accagtaccg tacatgcatc
     2101 cggacgagat ttacagcgcc tggcggtgcg tctggctgat tatcctggtg caagtgcact
     2161 tgatatgggc tgcggagcag ggcatgccag ctttgttgct gcgcaaaacg tgagcgcggt
     2221 ggtggcgtat gacttatctg cccaaatgct ggatgtcgtg gcacaagctg ccgaagcccg
     2281 gcaactgaaa aatatcacca cccgccaggg atatgccgaa agtctgccat ttgccgataa
     2341 cgcatttgat attgttatca gccgttattc tgcccatcac tggcatgatg ttggtgcagc
     2401 actgcgtgaa gtgaaccgga tattgaaacc tggcggtagg atgattgtga tggacgtaat
     2461 gtctccgggt catccagtgc gcgacatctg gttacagacg gtagaagcat tacgcgatac
     2521 ctctcacgta cgaaactacg ccagcggtga gtggttgagg ttaatcaatg aagccaatct
     2581 gatagttgat aatttaatta cagataagtt accgctggaa ttttgttcat gggtcgcgag
     2641 aatgcgtacg ccagaagcgt tagtagacgc tattcgcatt taccaacaga gcgcatcgac
     2701 agaggtgaga acgtattttg ccttgcagaa tgacggctct ttcaccagtg atatcatcat
     2761 ggtagaagca cataaagcgg cataaataaa aaaggcaccg ggggaatcgg taccttttta
     2821 ttatctggtt tgtcaggaat ctggcatgtt gttgtttttc acaaacaacg tcagcttgtc
     2881 gcctggttgc agattcgcag tgtcgctgtt ccagcgcatc acatctttga tgttcacgcc
     2941 gtggcgttta gcaatgcttg aaagcgaatc gcctttgcgc acacgatacg taatgctatc
     3001 gctgttgttt gccagtcgct gtgcaccaat cgtcaaactt tggcctggct tcagcttaga
     3061 tccgcgcagt ttgttccact gctgcaaatc tttggtgctt acgccgagac gtgaagcgat
     3121 gcttgaaagc gtgtcgccag agcgtacggt gtaaacacgg ctgttaagcg gcgtattgtt
     3181 ggcaaccagc gtcgactgta cagcagcaat ttcgcctgaa gcgagagatt cacgcagttg
     3241 atctgcatgc ttctttggca ccatcacgta ctgcggccca cttgcgccca gcgtggagcc
     3301 tttcacgcca gcgttgaatg tcttcagctt gctgacggaa atccccgcca tatctgcaac
     3361 cttcgccatt tcaaccgggc tactcaggtg cacacgcgcc agagcacggc tttcatcggt
     3421 cgttggcaga cgtacgccat aacgcttgct gtttttgaga atatcactca atgccagcat
     3481 tttaggcacg tactgcttcg tttcctgcgg caacggtaac gaccagaagt ccgtggattt
     3541 cccacgcgct ttgttcgttt taattgcctt catgacccga ccttcgccgc tgttataagc
     3601 cgctacggtc agaagccagt cgtcgtcaaa cattttgttc agacgctgca tcatattcag
     3661 cgcggcagtt gttgaagcaa caacatcgcg acgcgcgtca taattgcggg tctgtttcaa
     3721 accataattg cgccccgtgc tcggaatgat ctgccagatg cctgcggcat tggcgccaga
     3781 cgttgcgtga ggatcaaaag cgctctccac tatgggtagt agtaccagtt ccataggcat
     3841 gttacgtttt ttaacttgcc ctgctatcca gtacatatac ggctctgccc gtaaagttac
     3901 atcgtggaga tagctcttat tgcgtaaata tttctgtttc tgttcgcgaa tccggtcatt
     3961 ttccggaatt cccatcttta gctcgtcgcc aatgaaagcc cacaagtcac catctggcgc
     4021 gatagacgtc ccatcgtcca tccatcgtgc ctgacttgta aactttgctg cttccccttg
     4081 accagctgca gaaaggctct gtgcgtgctg ttgaacgttg ccggtactct ggcaacccac
     4141 gagcaggaca gaggcgagta atatcgcttt tgccttcatg tgtgtgtcaa tagttgctta
     4201 aaagacgacc gatcataacg gcgaacggag ccgatgacaa gaaagtttta tcagaaccta
     4261 tctttctttg accttaacca tgcaaaacgc tcttcaggtt gttgcaataa tgtttcttca
     4321 ttaattccat taattaaatc aatatcttcc gttcttaaaa aaacattaat ttgccgctca
     4381 tttttcagaa ttacgggtag tgttatttga ttttttgccc gtaactcctt aactttacga
     4441 taataatcat ttatggacaa atcgtgcgga aaaatactca aagcaaactt catatttgat
     4501 aacgtatatt catgagcaca acataccaat gtatcgtcag gtaacgcact tagcttttta
     4561 agtgattgat acatttgtaa agctgtccct tcaaacaacc gaccacaccc accagaaaac
     4621 agcgtgtcgc cgcaaaatag ataaggttta ctgaagtaac agatatgtcc taaagtgtga
     4681 cccggtgtgg taattacact aaattcatgc cctaaaacga aggcagtttc gccatctttg
     4741 actacctgtg ttgttccctt atcttgtgtc tcttgtggac catacactac aatttgcgga
     4801 aacttttcca ccagttcttt tacgccgcca acgtgatcgt ggtgatggtg ggttagaaat
     4861 atggcctccg gttgccagtt attggcggta atggcgttta atactggctc tgcgtctccg
     4921 ggatcgacaa tcaggcagcg acctgcctca tcattcaaaa cccagatgta attgtcatca
     4981 aaggcgggaa tactgttaag attcatagat tacctctcag tgtaaaacgg aaggttgtga
     5041 tgaaaccggc aagagtccct caaactgtcg tggctcctga ttgctggggc gatttgccct
     5101 ggggagagct ttatcgcaag gcgctggagc gccagctcaa cccgtggttc actaaaatgt
     5161 atggttttca tctgcttaag attggcaatt taagcgcaga aatcaattgc gaagcgtgcg
     5221 cggtttctca tcaagtgaat gtttctgcgc aaggaatgcc cgtccaggtg caggcggatc
     5281 cacttcatct tccttttgcc gataaatccg ttgatatttg tctactggca cataccttgc
     5341 cgtggtgcac cgatccgcat cgtttattac gtgaagccga tcgggtactg attgatgatg
     5401 gctggctggt gattagtggc ttcaacccca tcagtttaat gggattacgc aaacttgtgc
     5461 cggtattgcg caaaacctcg ccctataata gccggatgtt tactctgatg cggcaactgg
     5521 actggctctc tttgttgaat tttgaagtgc tgcacgccag ccgtttccac gttctcccgt
     5581 ggaacaaaca cggaggaaaa ctattgaatg cgcatattcc tgcgcttggt tgcttacaac
     5641 ttattgttgc ccggaaacgg actattcctt taacgctaaa tccgatgaaa cagagtaaaa
     5701 acaagccacg aattcgccag gcggttggag ccacccggca atgtcgtaaa ccacaggctt
     5761 aaacttcgac ttggtagccc gtatcttcca gtgtgggatt catcgccgcg gcacgagcca
     5821 gttcatcaca gcgttcgttt tccgggtgtc cggcatggcc tttaacccat tcccatttga
     5881 tttgatgctg ccccaatgca gcatcaagac gttgccagag atcgacattt tttactggtt
     5941 ttttgtctgc tgttttccag ccacgttttt tccagttatg gatccactgg gtgataccct
     6001 ggcggacata ctggctgtcg gtactcaaaa tgacttcgca atgttctttt aacgcctcca
     6061 gcgcgacaat agcggccatc aactccatac ggttgttggt ggtgcgggtg tagcctgcgc
     6121 taaaggtttt ctcgcgtccg cgatagcgta aaatagcgcc gtaaccccca ggtccaggat
     6181 tacccagaca cgaaccatcg gtgaaaattt ctacctgttt aagcatctct ggtagacttc
     6241 ctgtaattga atcgaactgt aaaacgacaa gtctgacata aatgaccgct atgagcactg
     6301 caattacacg ccagatcgtt ctcgataccg aaaccaccgg tatgaaccag attggtgcac
     6361 actatgaagg ccacaagatc attgagattg gtgctgttga agtagtgaac cgtcgcctga
     6421 cgggcaataa cttccatgtt tatctcaaac ccgatcggct ggtggatccg gaagcctttg
     6481 gcgtacatgg tattgctgat gaatttttgc tcgataagcc cacgtttgcc gaagtagccg
     6541 atgagtttat ggactatatc cgcggcgcgg agttggtgat ccataacgca gcgttcgata
     6601 taggctttat ggactacgag ttttcgttgc ttaagcgcga tattccgaag accaatacct
     6661 tctgtaaggt caccgatagc cttgcggtgg cgaggaaaat gtttcccggt aaacgcaaca
     6721 gcctcgatgc gctatgtgcc cgctacgaaa tagataacag taaacgaacg ctgcacgggg
     6781 cattactcga tgcccagatc cttgcggaag tttatctggc gatgaccggt ggtcaaacgt
     6841 cgatggcttt tgcgatggaa ggagagacac agcagcaaca aggtgaagca acaattcagc
     6901 gcattgtacg tcaggcaagt aagttacgcg ttgtttttgc gacagatgaa gagctggcag
     6961 cacatgaagc ccgtctcgat ctggtgcaga aaaaaggcgg aagctgcctc tggcgggcat
     7021 aaatacatgt aaaaggcgct aaaaatagcg acttgggcga tttttgcagc aaacgattca
     7081 aaagatgaga aaaatcgttg acgaaggtcg aggcaatccg taatattcgc ctcgttccca
     7141 acggaacaca acgcggagcg gtagttcagt cggttagaat acctgcctgt cacgcagggg
     7201 gtcgcgggtt cgagtcccgt ccgttccgcc aatattcaga aagcccgaat cagtaatgat
     7261 tcgggctttc gtcgttttgg tgaagtgaga atttttcact ccgaagtaaa acggatatga
     7321 tttaaaaaat ggccagacat tataaattct ggccacttct gttactccag cgcatcccat
     7381 ttctccagca gttctactgg aatttcattt attacctccg agacgcgttt tccgaccagg
     7441 cgttctgcct ggcgcagcaa aggaatggtc gggctgctcg gttcgctgtg ctcaaaccag
     7501 cgacggatga cacgcaggcg ttccagcgcg tcgtttcggt cgcggatctg ccggggttct
     7561 gcgccggtca tcggtgatat ttcctccgcg atatgcaccg gtagcgcaat ctgctctgcc
     7621 tgcatctgtt cagtatgaac gatggtggcg ttttccggct gctcagcctg tggttgtggc
     7681 aggatctccg gcgtggtgct ctgcactgct cccggtaaca gggccagcag ttgcgtcaga
     7741 cgggagaagt ccggtgcctg gtcattcagc gtttcacgcg catggcgttg cagccgctca
     7801 gtggtctctg ctgcctggcg aaaggcatcc agcggcagtg tgccgcgcgc ttccaggtct
     7861 gccagttgct ggcgtaccga ttctggtgcc agcgcatccg ccgggcgtaa tgcagatagc
     7921 gaacgctcca catcccgcac ctgaaggcgc atggcagcat tatttgacag tgtaatgcca
     7981 cgaatatccg ccattacacc ttcgtggtcc agcaacgccg ccagcgcgtt actgcgtgcc
     8041 accgcagcat cttcggcggt gatatcttca gtcgcgagca gttgaggatg gatggcgtcc
     8101 gggtacgtgg agcacaacgt ttccagcagc gtgagcgcct ccgacagtcc cctggcaccg
     8161 gcctgctgga tacggcagcg cagcaggaca accagaatgc ggatatcttt gctgcgcatc
     8221 agcagacgat gggcgtcccg ctcaagctcc gcccagttaa tgttttccgg cgtactgaca
     8281 aaatcgccat attgcgcttc ttcacgcggt gcggcgcggg tgaacagcag caggtattcc
     8341 ggatcgtact ccagatccgg gccgcagggg cattccgccg aaattgggtc agacaggaat
     8401 atttccattt aattatcctc agtgcgccag cgtggtggcc agaccgtccg gattgctgga
     8461 ctcacgcacc agatgggccg atgccgccag acgagcgtga tctggcaacg ggctgtcagc
     8521 gctctcccgc aaatcctgcc cgctgaggct ggcgacataa aaggcaaagc ggtcatgttg
     8581 cggtttatcc agaccgcccc cagcggactg ggcgctgcgc catgcctggt aaaactgcgt
     8641 cagccagcgg tgatgaaaaa gatcagaaaa acgggtcagc gtcggctttc agtccggtta
     8701 cgcgccagtt cggtgtagtg cagcggcagt ggaccgttcg gcccccacag cacgaggctg
     8761 tacagactga tgtgcaggcg cccttcctgc cagcccacac tggcgatttc tcgtggcgca
     8821 aaggtcagcg cgggcgtttg tccgaggcga aatttttcca tccgtggttg ccaggtgttg
     8881 cccgccggaa cggtacacaa ctgcacatcc acgcggcgca tcaggttcag aaaaccgtaa
     8941 cgccaggggg gggcctgcgc ctgagtgaaa tcgttgctca tacgcttccc cgctgtcccg
     9001 ccctgacagg ccaggtcata attttgccgc gctgcatgct gtgcagcgtc atctgcgaga
     9061 aggtattaat ggaaacatgg cgggcgatat agtgttccag caccaggccg aacagatacg
     9121 gactgatgcc ggaaaaacct tcttcatcca ccgtcagttc acaactgacg ccacgcccat
     9181 agaccagtag tccggaaccg ggcagacggc gggtgaccgg ggtcgtttta caaccaatca
     9241 ggctgcgcac ctggcgcgat tgcggactgt catgagcagg aataaacagg ttcagcagat
     9301 ctcgcaacgc ctggccgccg gtacggtgat ccagatcggc cagcggcaga taattaaaag
     9361 ataactggcg gatcaaccgc catgccatct cacgttcagc catcgggggc tgcggcgagc
     9421 gtggcgggcg gataagccct acgcccgcca ccgggatggc tgcatccacg gttagatcgt
     9481 cccggccatt gcgggcaata agacagggca gatcgcggtt agtgaccatg gccgtgatgg
     9541 tgatgtggcg cagattttcc gggtacggcg cttcatactg gtcaaccagc gacaggaaca
     9601 cttccgagcc cgtatagggg gtacgggtgc cgtaacggcg ggcgttttct gataagcggc
     9661 ggggctcccg gcgcagggaa aaataacgtc cgtgattgcc ttcatcatta ttgcgggtgt
     9721 ggtagagcgg gcgaaaggcc atcttccgtg tggtgtcggt ttccagccct tccacttcct
     9781 ggaccgagaa cacctcataa tccagcggat gggttctgtc caccaccaga tgctgttccg
     9841 tcgtgctgtg cgtgacatcg atccgggcgg tgacgcgggg aaacaggttg ataaccgggg
     9901 tacagaacag gctgaactgt gccgcatttg tctggtggat cagccagtcc ggcggcaggc
     9961 ggttcagcag gataacaatc tccgccacgc caccgtcaat tttctgtaat ccggcagaca
    10021 ggcctgtcgg ggtgaaaaaa tagaagcgtt ccggacaggc aaaatattca tgcagcaggt
    10081 tatgaccgtg aaacacgttc cacgccagcg gcagcagccc ctgatccggc tccaggcctt
    10141 cgtacataac gggctgttgc agattcacat ccagcgcgcc gtcaaaatgc cccggaatgc
    10201 cagccagggg cgcgatggcg ctggtatgta gaagttctaa caggtgtgag gccgtacgct
    10261 cctcgccgca gagataaaac ggcagcctgt ccagtccggc aagctgactg aacgttagtt
    10321 caccaaaggt gcgcagggta atacgcagcg ccccggcgac gtgaatgttg gccggcaggt
    10381 agcggtgcag ggcaggcata tccggtggcg cggcggtcag tcgcgcctct tcgatggcga
    10441 gcggccacag cgttacatcc tggctgctgc gaaactggca ggcagtgctt tcgccttccg
    10501 gaatggcgga gaaaaaggct gtgtcgcgcg ggacggtaaa tcctttcgcc agatcgcctt
    10561 cttccgtatc gggatgcaac tgcgccacgc tcattgaggg cgtgggtgtg acgtaattgg
    10621 ggctgaccac ttccagcagg cgctgggtga aacgggggaa ttcagcgtca atttttagct
    10681 gcgtgcgggc ggtcaggaag ctgaaggcct cgaccattcg ctctacatac ggatcggcaa
    10741 tatccgttcc ctgcatgccc agccgggcgg cgactttggg atgacgggcg gcaaattcgg
    10801 ctcccgtttc ccgcagatag ctcagttcgc ggttgtagta ttccagtagc cgtggatcca
    10861 tcggtctctc tctgaattaa aaaaacgtaa tgcgactctg ctccatatcc agcgcgctac
    10921 gtacccggaa tgcagtcgga tacggctgcg tgaggatctc gccgcgaatt tcaaactgta
    10981 atgtgttatg gccactctgg cggcgctcat cctgaagtgg gatgatttgc agagtggacg
    11041 ctttcagccg gggttcaaac cgggtgatgg ttcgcaaaat ggccctgcgg atatcgtccc
    11101 acttgtgctc atacagaaag ctgcccgcca gtggcggcag gccgtagtta agtacggacg
    11161 ctgccgcgta cggataacgt gcagcatcaa tatcatcttc atggctgacg gtgttgagca
    11221 gaaatgacaa atcccggcgg atgatctcct taatctgaac cggactgacg ctgataccgt
    11281 ggtcgcgttt ctggtttggg gcgtcgtcac acaggcggtc aaacagcgtg ggcagcaggt
    11341 gattgcctgg acgctggcgg gaagcgctca cgcgtcgctt ccttcagcgg tgttaaaggt
    11401 acaggtggac aaatccagca ggctgatatc gccgtgaccg gtgagccaca ccctctgacc
    11461 cagcgcctgt accagtgtct ctcccggacc gtcctgccag accgtttccc ggcacagacg
    11521 cagggagtcg gcagcctttt cagagccgct gtagcgggga aacagctagg cactgtgtgt
    11581 atcgccattg ttcagggtga cgttgacggg tttccagatc aaatcgatca gatgcgcagg
    11641 ccgcggcgat ttcagcgaac agatttgtga aaacggcagc cagatatatg ctccgcccgc
    11701 gatcagttcg agtaccggac cggtacggga gtcgctgtcg ctaatccagt caaatgcgcc
    11761 gccattccac tgcccggaag tgtctgttat ggcctcaagc gcgcggctgc ggtgagcatc
    11821 agcttcctgc gcttcaccac gggcgttaca ggcgagggcg gtcagcagat cctccatcca
    11881 ggcgggcggc gggagaataa caccgggccg ctgttcgccc tgaaagcagg cgtggcggta
    11941 tatctcacag cggatcagct cgccaaatac ctgcgcttcg cgagtataat ttgcgtccat
    12001 ccgggcgcat agctggatct gttgcagtgc ccgcgaccag tcgcctgcca cgcacaataa
    12061 ctgaaacagg ctgtggcggc gggaggcctg cgctgggtgc atcctgacct gttgctctgc
    12121 catctgaatg ccctccacca cggaataatc ccgcagcagc gccgacaagg taatcggtag
    12181 ctcgttattg tttgtcatga tatatctctc catgttgtgc cgggtttatc cggtaatggc
    12241 tgtcaatgcc aatgatgcga tgctctttcc gtgtcagatc tggcagtacg gtgttatgcg
    12301 ctgtttccgg ggcgtattcc ggggaaagta aatgcaggat atcaggggga gtcttttcga
    12361 caggccagat agtttcatct tcgccagtgg catccagtac attgagaata gcgtcaatgc
    12421 ttaacccacc agtgaccata tcctgcaatg tctcggcttc ggtgaccagg tcacgcaatg
    12481 aatccagatc ctgcgaggag ggctcttcgt taaaaggaaa aggatcagca tggcgattaa
    12541 gatcttgttc gggagataac aacgcctcgc ggtattcctg ataaagtcgg gtcagggcat
    12601 ttcctttttc cgcaccttct ccggtggagg aggctccatg aacaggaatg atatcgaatg
    12661 gattttgtga tgtcagatgc tgctgcctga accaatccag atccagatat tcggttattg
    12721 cttccgacgt gatggcagtc tgcgatatcg tttttactgt gctcaggttc ggagatgatg
    12781 gattgccatg tacaaggcac tatgaagata acccccattc tatcaggtcg ccttcattca
    12841 gacgcatacg gtgatgaggt tccacaatct ggtcgtttac cgtacagcaa tattcggtag
    12901 actgattgat aatccaccat gcttcttcgt ggcgggtcag agtgagggca atctttacat
    12961 catcattcac cttaaaaaca ggaatccagg gagaggtaac ggtgaagata atttgtgttc
    13021 cttcagtcgc gtaaattgtg ttatcggcga tcccgtgcgc ctgtattttt tggagtcccc
    13081 acgtaatatt tggtttcatt gtgt
//
LOCUS       DAZSNO010000100        12899 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZSNO010000100 DAZSNO010000000
VERSION     DAZSNO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12899
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.100"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..203)
                     /locus_tag="ACHWN2_002780"
     CDS             complement(<1..203)
                     /locus_tag="ACHWN2_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFR8932242.1"
                     /translation="MAISDTKLRTIYGKPYSGPQELADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDAR"
     gene            complement(409..1128)
                     /locus_tag="ACHWN2_002781"
                     /pseudo
     CDS             complement(409..1128)
                     /locus_tag="ACHWN2_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1144..1476)
                     /gene="cusF"
                     /locus_tag="ACHWN2_002782"
     CDS             complement(1144..1476)
                     /gene="cusF"
                     /locus_tag="ACHWN2_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR8932243.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1500..2882)
                     /gene="cusC"
                     /locus_tag="ACHWN2_002783"
     CDS             complement(1500..2882)
                     /gene="cusC"
                     /locus_tag="ACHWN2_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR8932244.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3039..3722
                     /gene="cusR"
                     /locus_tag="ACHWN2_002784"
     CDS             3039..3722
                     /gene="cusR"
                     /locus_tag="ACHWN2_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR8932245.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3712..5154
                     /gene="cusS"
                     /locus_tag="ACHWN2_002785"
     CDS             3712..5154
                     /gene="cusS"
                     /locus_tag="ACHWN2_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR8932246.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5304..7541
                     /gene="nrfB"
                     /locus_tag="ACHWN2_002786"
     CDS             5304..7541
                     /gene="nrfB"
                     /locus_tag="ACHWN2_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFR8932247.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7528..10496
                     /gene="nfrA"
                     /locus_tag="ACHWN2_002787"
                     /pseudo
     CDS             7528..10496
                     /gene="nfrA"
                     /locus_tag="ACHWN2_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10497..11387
                     /locus_tag="ACHWN2_002788"
     CDS             10497..11387
                     /locus_tag="ACHWN2_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR8932248.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11570..12331
                     /gene="envY"
                     /locus_tag="ACHWN2_002789"
     CDS             11570..12331
                     /gene="envY"
                     /locus_tag="ACHWN2_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR8932249.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12377..12453)
                     /locus_tag="ACHWN2_002790"
     tRNA            complement(12377..12453)
                     /locus_tag="ACHWN2_002790"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12417..12419),aa:Arg,seq:tct)
     gene            12696..>12899
                     /locus_tag="ACHWN2_002791"
     CDS             12696..>12899
                     /locus_tag="ACHWN2_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR8932250.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3156 a         3346 c         3276 g         3121 t
ORIGIN      
        1 tgtctggcat ccttcaaaga cagggatggg tatcgaccaa gcccaagtcg attaggcttg
       61 ccatgccagc gatagcggta ctggaactgg atgaccccct tcggtgaaat tcgtacgctg
      121 aggccatcag catcagccaa ttcttgtggg ccagaatatg gtttaccata aatagtacgc
      181 agttttgtgt cgcttatagc cataaataat atttggtacg ctcagaaata atggtttggt
      241 acacatctgg tacacaatat cacatggaca acagcgcaca gtaaacaact atatttgacg
      301 ttcgtagaca tactcaggtg aaaaaaaggt tgtttttcga gttataacag acagttaatt
      361 aactacagtg gacaatgcta gacaatgaca gacacagata agcaacctac cttcctcttt
      421 cgcgaccacg ttatctttgg cgatattcat tcccgcgcgc agatcaaacg cggtgatcac
      481 gccatcaatg ggcgctttga gcgtaaagcg agtctggatt ttttgcgtgg cgatcaggtg
      541 gcgaatatcc gcctccggca ttcccgccag tcgcagccgc tcaagaatgc cttcagtctg
      601 ggtcgccgta ccgccggttt cgcgcaacag taaatactca ctctgcgctt ccacccagtc
      661 gggaatggtc aggtcgagaa gcggtgtgcc cttttgtact ttatcgccca cggtaagcgg
      721 atacaccttg tcgataaacc cggcagcgcg agcctgcaca atggcatact gatactcgtt
      781 gtaactgaca ttcgtcggga aactctgggc aaaagtcagc ggcccgcgcg tgacggtagc
      841 cgttttcacc cccagattct gcgtctgagt cgggtcaatg cgcacaccag acgcagaact
      901 ctcttcatcg gcatatttcg gcaccagatc catatccata aacggcgatt tacctggttt
      961 atcgaaccgc gtattgggat acatcgggtc gtaccagaat aagattttac gttctgcggt
     1021 cggcattttt tctgcgtgcg gttccgcctg gtcaaaccag gtaaaacctg ccgctgaaat
     1081 aataccgccc gcgatcatgc tgccgataat aagcgcgatt tttttcatct cattaaacct
     1141 gggttactgg ctgactttaa tatcctgtaa taaagaaagg ttgccctgct ggacaaaatt
     1201 aaacgccact ttgtcgccgg ttttaatttc actcattttc gtctgcgggg tgatggtaaa
     1261 gcgcatggtc atctccggcc agttgacggc agcaatcgga tcgtgatgga tggtgatttt
     1321 tttgctttcc agatcaacac cttttaccac accggtggca ctaatgacct gtggttgtgc
     1381 ttcgctcatg gtttcatgat gatgttcgtt agcctgggca ttaaagccaa taacggtaaa
     1441 cagactgaac attgcgactt gcagtgcttt tttcatttat tctctcctgg agttaaaaat
     1501 tattgctgcc aaccgccccc taaggcggta tacaaataaa tttcgttaac ctgacgggca
     1561 taattcaggt caagtaaagt ttgtcgggtt gcaaataaag aacgctcggc atccagcact
     1621 tccagataac ttaccgcgcc gtgctgatat aatgcccgcg cccgttgcag ggtaatttgc
     1681 agcgacgcca gataacgctg ctgggcgctg atttgatcgt ccaggctttg acgtaatgcc
     1741 agcgcatctg ccacctcttt aaaggcgttc tggattttct gttcataatt caccaccgac
     1801 tgctgctggc gaatttcggc gatatccaga ttggcctggt tgcgtccggc attaaaaatg
     1861 gggatctcaa ttttaggaat aaaattccac atcccgctgc tagcgttaaa taatgacgac
     1921 agatcgctgc tggcggttga tattccgctg gtcaggctga tagacgggaa aaatgccgca
     1981 cgcgcagcac caatattagc attagccgcc attaacgcgt gttcagcttc cataatatcc
     2041 gggcgctgca ataagatttg cgacgacaag cccgccggta atttaacgct ttgcaggctg
     2101 ccgctgttta ctgtctgcgc ttgcggtagc ttgccgtagc ttcccaataa cagttgcaat
     2161 gcattattcg cctgcgccag ttccccctga cgtttagcga tgtcactgcg ggtactttct
     2221 atcaccccgc gagcctgttc cagcgccaga acattgctgc taccggtcaa cagttgtttt
     2281 tcgacaaacg catatgactg ctgataatta cgcagcgttt cttcggctat ttgcaattgc
     2341 gcatacgcca gctgctgatt gaaatagctt tgcgcgacat tagaaaccag caaaatatgc
     2401 accgcgcgct gggcttcctc agtggctaaa taattttggc gctcggcttc gctcatgttc
     2461 tttaagcgac cgaaaaaatc gagatcaaag ctggcgttaa ggccagtcga gaactcccgc
     2521 gtcgtggctg aatcgccttt aagattgccg ctccagctgc cgctgccctc gccattgagc
     2581 tgtgggtagc ggtcggcatc ggtcagacga tattgcgccc gtgcctcctg cactttcagc
     2641 gtcgccatgc gcaaatcccg gttattcacc agcgcctcgc taatcagcgt cttcacctga
     2701 ttatcaacaa aaaaggtgcg ccagcccacg ttctggtagt tatccgctgc gttaaccagg
     2761 ccgttctggc tgagtgagaa ctgctgcggt acgggcatta ccggacgctg ataatccggt
     2821 gccagtgaac aaccagttag cgcaagggcc acacaaaatg gcagaagttt acaaggagac
     2881 ataggctcat aatttctggt gattttatgt cgccaacttt actcgccagg ctctgatttt
     2941 ccggtgacag gaaaatgaca aaattgtcat tttgccaata agcgattgcc atctgatccc
     3001 gctactctag aattgcccgg gcaacatgcg gaggaaatat gaaactgttg attgtcgaag
     3061 atgaaaagaa aaccggagaa tacttgacca aagggttaac cgaagccggt tttgtggtcg
     3121 atttggccga caacgggctg aatggctacc atctggcgat gaccggtgat tatgacctga
     3181 taatcctcga tattatgctg ccagacgtga acggctggga tatcgtgcgc atgttgcgct
     3241 ccgccaataa agggatgccg attctgttgc ttaccgcgct tggcaccatt gaacatcgcg
     3301 tcaaggggct ggagttgggg gcagatgact acctggtgaa accgttcgct tttgctgaac
     3361 tgctggcgcg ggtgcgcact ctcctgcggc gcggggcggc ggtgattatc gaaagtcagt
     3421 ttcaggttgc cgatctgatg gtcgatctcg tcagccgcaa agtcacccgc agcggcacgc
     3481 gcatcactct gaccagtaaa gaatttactc tgctggagtt ttttctccgc catcagggcg
     3541 aagtgctgcc ccgctcgctt atcgcctcgc aggtatggga catgaatttc gacagcgaca
     3601 ctaacgccat tgatgtggcg gtgaagctgc tacgcggcaa aatcgacaac gactttgagc
     3661 cgaagctgat tcaaaccgtg cgcggcgtgg gttacatgct tgaggtgccg gatggtcagt
     3721 aagccatttc agcgcccgtt ttcgctggca acccgcctga ccttttttat cagccaggcc
     3781 accatcgcgg cgtttttcgc ctttgcatgg atcataatcc actcagtaaa agtgcatttt
     3841 gccgagcagg acattaatga tttaaaagag attagcgcca cccttgaacg ggtactaaat
     3901 caccctgacg aaacgcaagc ccgacgctta atgacgctgg aagatatcgt cagtggttat
     3961 tccaacgtgt tgatttccct ggcagatagc cacggcaaaa cggtgtatca ctcccccggt
     4021 gcgcctgata tccgcgagtt tgcacgtgat gccatacccg ataaagacgc gcggggtggc
     4081 gaggtgtatc tcctttccgg cccgacgatt atgatgccag gccacggtca cgggcatatg
     4141 gaacacagca actggcggat gattaacttg ccggttggcc cgttggtgga cggcaaaccg
     4201 atttatacgc tctacatcgc gctgtcgatc gattttcatc ttcattacat aaatgatttg
     4261 atgaataaac ttattatgac cgcatcggta atcagcatcc tgatcgtctt tatcgtgctg
     4321 ttggcggtac ataaaggtca cgcgccgatc cgcagcgtca gccgtcaaat ccagaatatc
     4381 acctcgaaag atctcgacgt tcgccttgac ccgcagaccg tgccgattga gctggaacag
     4441 ctggtactgt cgttcaacca tatgatcgag cgtattgagg atgtctttac tcgccagtcc
     4501 aatttctcag cggatatcgc ccacgaaatt cgcacaccaa ttactaatct cattacgcaa
     4561 acggaaatcg ccctcagcca gtcgcgcagc cagaaggagc tggaagatgt gctctactct
     4621 aatctcgaag agctgacgcg aatggcgaaa atggtcagcg atatgctgtt tctcgctcag
     4681 gccgataaca accagctaat ccccgaaaag aaaatgctca acctggcgga tgaagtcggc
     4741 aaagtgttcg attttttcga ggcgctagcg gaagatcgcg gcgtggagtt gcggtttgtt
     4801 ggcgacaaac gtcaggttgc gggcgatccg ttgatgctgc gtcgggcgct aagcaacctg
     4861 ctttctaatg cccttcgtta tacgccaacc agagagacaa ttgtagtacg ctgtcagacg
     4921 gtcgatcacc aggtgcaagt ttccgtcgaa aaccccggta cgcccattgc gcccgagcac
     4981 ttaccgcgat tgtttgaccg tttctatcgc gttgacccct cccgccagcg aaaaggtgaa
     5041 ggtagcggta ttgggctggc gatagtgaaa tcgattgttg tcgcgcataa aggcacggtt
     5101 gcggtaacgt cagatgcgcg ggggacaagg tttgttatca cattacccgc ttaattattc
     5161 agcaaacggc aacttttata accagtgtaa aaataacgtg ccgcaatagt catacatatt
     5221 aatggtaaaa agctgtcaca attcataaaa aaccttaata tacgccaccc taaacataac
     5281 cagcgttaat gtaaggtttt tgtgtggact ggcttcttga tgtttttgct acctggctct
     5341 acggcttaaa agtaatcgcg ataacgttag cggtcatcat gttcatcagc gggctggacg
     5401 atttttttat tgatgtcgtc tactgggtac gccgcattaa acgcaagttg agtgtttatc
     5461 gccgctaccc gcgaatgagt taccgcgaac tgtataaacc agatgaaaaa ccgttagcga
     5521 ttatggttcc ggcgtggaat gaaacgggcg tcatcggcaa tatggccgag ctggcggcga
     5581 ccacgctcga ctacgaaaac tatcatatct ttgttggcac ctaccccaac gaccccgata
     5641 ctcaacgtga tgttgacgaa gtgtgcgctc gctttccgaa cgtgcataag gtagtctgcg
     5701 cgcgtcctgg tcccaccagc aaagccgact gcctgaacaa cgtgctggac gccatcaccc
     5761 agtttgaacg tagcgccaat ttcgcttttg ctggttttat tctgcatgac gccgaagatg
     5821 tgatttcacc gatggaattg cgtctgttca actatctggt cgagcgtaaa gatctgattc
     5881 agatcccagt gtatccgttt gaacgcgaat ggacgcactt caccagcatg acttacattg
     5941 atgagttttc agaactgcat ggcaaagatg ttccggtgcg tgaagccctc gccggacaag
     6001 tgcccagcgc aggcgtcggc acctgtttca gccgccgcgc cgtgaccgca ctgttagctg
     6061 acggtaacgg tattgctttc gacgtgcaga gtcttactga agattacgac attggcttcc
     6121 gcctgaaaga aaaaggtatg acggaaattt ttgtccgttt tccggtggtg gacgaagcca
     6181 aagaacgcga gcagcgtaaa tttttacagc acgcacggac gtcaaacatg atctgcgtgc
     6241 gcgaatattt ccccgatacc ttttcgactg cggttcgaca aaaatctcgc tggatcatcg
     6301 gcattgtttt ccagggcttt aaaacccata aatggacctc cagcctgacg ctgaactact
     6361 ttctctggcg cgaccgcaaa ggggcaatca gtaactttgt cagcttcctc gcgatgctgg
     6421 tgatgatcca gcttttgctg ttgctggcgt atgaaagttt gtggcccgat gcctggcatt
     6481 tcctttctat ttttagcggc agcgcatggt taatgaccct gctgtggcta aactttggct
     6541 tgatggttaa ccgcatcgtg cagcgagtga ttttcgtcac tggctactac ggcctcacgc
     6601 agggcctgct ttccgtcctg cgtcttttct ggggcaacct gattaacttt atggctaact
     6661 ggcgcgtgct gaaacaggtt cttcagcacg gcgatccgcg tcgcgtcgcg tgggataaaa
     6721 caacgcatga cttccccagc gtcactggcg atacccgctc gttgcgcccg ttaggtcaaa
     6781 ttctgctgga aaatcaggtc atcactgaag aacaactcga tacagcactg cgtaatcgcg
     6841 tcgaaggtct acgcctgggc ggttcaatgc tgatgcaggg gctgattagc gccgagcagc
     6901 tggcacaggc gctggcagag caaaacggcg tggcgtggga atccatcgat gcctggcaga
     6961 tcccgtcctc gctgattgcc gaaatgccag cctccgtagc gctgcattat gcagtactgc
     7021 cgctgcgtct ggaaaatgat gagttaattg tcggcagtga agatggtatt gacccggttt
     7081 cgctggcggc cctgacgcgt aaagtcggac gcaaagtgcg ttacgtcatt gttctgcggg
     7141 gacaaattgt cacagggtta cgccactggt atgcacgccg acgcggtcac gatccgcggg
     7201 caatgttgta caatgcggtt cagcatcagt ggctcacgga acagcagacc ggtgaaatct
     7261 ggcggcaata tgtgccgcat cagttcctgt tcgccgaaat actgaccacg ctcggtcata
     7321 ttaatcgttc agcaattaac gtgttgttat tgcgccatga acgcagttct ctgccgctcg
     7381 gcaagttttt ggtcaccgaa ggcgttatca gccaggaaac gttggatcgc gtcctgacaa
     7441 ttcaacgcga attacaagtt tcgatgcaat cactattact caaagcaggt ttaaacacag
     7501 aacaggttgc gcaactggag tccgaaaatg aaggagaata accttaatcg cgtcatcgga
     7561 tggtctggtt tactgctgac gtctttattg agtaccagcg cactcgcaga caatatcggc
     7621 accagcgcag aagagctggg gctgagcgat tatcgccatt ttgttattta tccccgtctc
     7681 gataaggcgc tgaaggcaca gaaaaataac gacgaagcaa ccgccatccg cgaatttgaa
     7741 tatatacacc aacaggtgcc ggataatatt ccgctgactt tataccttgc ggaagcctat
     7801 cgtcattttg gtcatgatga ccgggcgcgg ctgttgcttg aggatcaact gaaacgtcac
     7861 ccaggagatg cccgacttga gcgcagtctg gcggctattc cagttgaagt gaaaagcgtt
     7921 acgactgttg aagaactgct tgcccagcaa aaagcgggcg acgctgcgcc gaccctgcgt
     7981 tgtcgcagtg aagtcgggca gaatgccctg cggctggcac aattacctgt cgccagagcg
     8041 caactgaacg atgcgacgtt tgctgcatcg ccggaaggaa aaacgctgcg aaccgatctg
     8101 ctgcaacggg caatctacct gaaacaatga tcccaggcag atacgctata caatgaagca
     8161 cgccagcaga acacattaag cgcggcagaa cgccgtcagt ggtttgacgt gcttcttgcc
     8221 gggcagctgg acgatcggat cctggcactg caatcacagg ggatcttcac cgatcctcag
     8281 tcatatatta cttacgcgac cgcgctggct tatcgtggcg aaaaagcacg cctccagcat
     8341 tatctcattg aaaataagcc gctgtttacc acgggcgcac aagagaaaag ttggctctat
     8401 ctgttatcta aatacagcgc caaccccgtt caggcattgg cgaattatac ggtgcagttt
     8461 gccgataacc gccagtatgt tgttggcgcg acgctaccgg tgctgttaaa agaaggtcag
     8521 tacgacgcag cgcaaaaact gctcgccacc ctccccgcca atgaaatgct tgaggagcgt
     8581 tatgctgtca gcgtggcgac ccgtaacaag gctgaatctc tgcgtctggc acgattgctg
     8641 tatcagcaag aaccgacaaa tcttccccgc ctggatcaac tgacctggca actgatgcag
     8701 aacgagcagt cacgcgaagc tgccgattta ttgctgcaac gctatccttt ccagggcgat
     8761 gcgcgtgtca gccagacttt aatggcgcga ctggcgtctc tgctggaaag tcatccttac
     8821 ctagcaacgc cggcgaaggt ggcgatttta tcgaaaccct taccgctggc ggagcaacgt
     8881 cagtggcaaa gtcagttgcc gggtattgca gataattgcc cggcaatagt tcgcttgctg
     8941 ggcgatatgt cgccttccta cgatgctgcc gcttggaacc gtctggcaaa gtgttatcgg
     9001 gacacgctac ccggtgtggc gttgtatgca tggcttcagg ccgaacaacg acaaccgagc
     9061 gcctggcaac atcgtgcggt agcctatcag gcgtatcagg ttgaggacta cgccaccgca
     9121 ctggcggcct ggcagaaaat cagtcttcac gacatgagca atgatgatct gctcgccgcc
     9181 gccaacaccg ctcaggcagc gggaaatggc gcagctcgcg atcgctggct tcaacaggca
     9241 gaacaacgtg agctgggaaa caatgctcaa tactggtggc tgcatgcgcg acgttatatc
     9301 ccagctcagc cagaactcgc actgaacgat ctcacgcgct caatcaatat tgccccttct
     9361 gccaactctt acgttgcgcg ggcgacaatt tatcgccaac gtcataatgt tccggccgcg
     9421 gtgagtgatt tgcgcgccgc gctggaactg gaaccaaata atagcaacac ccaggcagcg
     9481 cttggttacg ccttgtggga tagcggtgat atcgcacagt cgcgggaaat gctcgaacag
     9541 gcgcataaag ggcttccgga cgatccggca ctgatccgac aactggccta cgtgaaccag
     9601 cgtctggatg acatgcctgc gacgcagcac tacgcccggc tcgtgattga tgacattgat
     9661 aatcaggcat aaccccactg accccagagc aaaatcagca acgcttcaat ttccgccgtt
     9721 tgcatgagga ggtcggtcgc cgctggacgt tcagtttcga ttcttccatc ggcttgcgtt
     9781 ccggcgcaat gagtaccgct aacaataatg tcggcggtgc agcgcctggg aaaagctatc
     9841 gtagctacgg acaactggaa gccgagtacc gcatcggacg caatatgctg ctggaaggcg
     9901 acctgctctc agtttatagc cgcgtctttg ccgataccgg agaaaacggg gtgatgatgc
     9961 cggtgaaaaa tccgatgtcc ggcaccggtc tgcgctggaa gccgctgcgc gatcagatct
    10021 ttttcctcgc cgtcgaacag cagttgccgc tgaacgggca aaatggcgca tccgatacca
    10081 tgctgcgcgc cagcgcctca ttctttaatg gcggcaaata cagcgacgaa tggcacccga
    10141 acggttcagg ctggtttgcc caaaacctgt acctcgatgc ggcgcaatat atccgccagg
    10201 atattcaggc gtggacggca gattatcgcg tcagctggca tcagaaggta gctaacggac
    10261 agactattga gccttacgct cacgttcagg acaacggcta tcgtgataaa ggcactcagg
    10321 gcgcgcagct tggcggtgtc ggggtccgct ggaatatttg gaccggcgag acgcactacg
    10381 acgcctggcc gcacaaagtc agtctcggcg tcgagtatca acataccttt aaggcgatta
    10441 atcaacgtaa cggagagcgc aacaacgcgt ttctcaccat tggagtgcac tggtaaatgc
    10501 gtaagttcat tttcgtattg ctgacactgc ttttggtcag ccctttttcc tttgcgatga
    10561 aaggtattat ctggcaacca caaaaccgag atagtcaggt taccgatacc cagtggcagg
    10621 ggctgatgag tcagttacgt tttcaaggct tcgataccct ggttttgcaa tggacccgtt
    10681 acggcgatgc atttacccag ccagaacagc gcacgttatt gtttaagcgt gccgcagctg
    10741 cgcaacagac tggcctgaag ctgattgtcg ggctgaacgc cgatccggaa ttttttatgc
    10801 accagaaaca gtcgtctgca gcgctggaaa gctatcttaa tcgcctgctg gctgccgatc
    10861 tccagcaagc cagattatgg agcgccgcgc ctggcataac gccggatggc tggtacatca
    10921 gcgcggaaat tgacgacctg aactggcgca gcgaagccgc ccgtcaacct ttgctaacat
    10981 ggttaaacaa cgcgcagcgg ctgattagcg atgtttcagc aaaaccggtt tatatcagta
    11041 gttttttcgc cggaaacatg tcgcccgatg gctatcgcca actgctggaa caagttaaag
    11101 caaccggagt taatgtctgg gtacaggatg gcagcggcgt ggataaactt accgctgaac
    11161 agcgtgaacg ttatctacag gccagcgccg attgccaaag ctccgcccca gcaagcggca
    11221 ttgtttatga actgtttgtc gccggcaaag gcaaaacctt tacagcgaaa ccgaaaccgg
    11281 acgcagaaat tgcctcgctg ttagcgaaac gttcctcttg cggtaaagac atgctctatt
    11341 tctctctgcg ctatttgccc gtcgcgcacg gcattctcga gtattaaatc ccctccaggt
    11401 aagtcgggta cgacctggct tacctctttc gctctcgtca gaattccatc aagattaatt
    11461 gctaaaaagc atgctaaaga aagaagatcg ttatcggcga acgggccgat cactcatgca
    11521 ggaagcatag atcctttgag cggagtttct gatatctgag gagtgcgaaa tgcaattgag
    11581 cagcagtgaa ccttgcgtgg tgatcctgac cgaaaaagag gtagaggtaa gcgtcaataa
    11641 ccatgctacg tttacccttc cgaaaaacta cctggccgcc ttcgcgtgca acaataacgt
    11701 cattgaactc tcaacgttaa atcacgtatt aatcacccac atcaaccgta acatcatcaa
    11761 cgattatctg ttgtttttaa ataagaaatt aacctgtgta aagccctggt cgcggctagc
    11821 aaccccggtt atcgcttgtc atagccgtac accggaagtg ttccggctag ccaccaacca
    11881 cagcaagcag caatccagca aaccctgcga agcggagttg acgcgcgcat tactttttac
    11941 cgtattgtct aactttcttg agcaatcgcg gtttattgcc ctactgatgt atatcttacg
    12001 cagcagcgtc cgcgacagcg tctgccgcat tattcaaagc gatattcagc attactggaa
    12061 tctgcgaatt gtcgccagtt cgctatgttt aagccccagc ctgctcaaaa agaaattaaa
    12121 aaacgaaaat accagctata gccagattgt cacagagtgt cgtatgcgtt acgccgtaca
    12181 gatgttattg atggataaca aaaatatcac tcaggtggcg caattatgtg gctatagcag
    12241 cacgtcgtac tttatctctg tttttaaggc gttttacagc ctgacaccgt tgaattatct
    12301 cgccaaacag cgacaaaaag tgatgtggtg aagggcaaag cggaaacgga taagacgggc
    12361 ataaatgaag aagaaatggc gcgccctgca ggattcgaac ctgcggccca cgacttagaa
    12421 ggtcgttgct ctatccaact gagctaaggg cgcgttgata ccgcaatgcg gtgtaatcgc
    12481 gtgaattata cggtcaaccc ttgctgagtc aatggctttt gatctggttg ctgaacaagt
    12541 gaacgaccgc gtctgatttt ctgatttatt tcgctatagc ggcaaacaaa cgcacaccgc
    12601 tgcgcgtctg aatcaagaaa acccgtattt tcatgtatca aagtacaatt taccgcccta
    12661 acggaaaatt gtccgctcct atgagactgg taactatgaa accaacgtcg gtgatcatta
    12721 tggatactca tcctatcatc agaatgtcta ttgaagttct gttgcaaaaa aacagtgaat
    12781 tgcagattgt cctgaaaacg gatgattacc gcataaccat cgattatctc cgaacccgtc
    12841 ctgttgattt aatcattatg gatatagact tgcccggaac agacggtttt accttcctg
//
LOCUS       DAZSNO010000101        12739 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZSNO010000101 DAZSNO010000000
VERSION     DAZSNO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12739
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.101"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1261)
                     /locus_tag="ACHWN2_002792"
     CDS             complement(<1..1261)
                     /locus_tag="ACHWN2_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR8932251.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRG"
     gene            complement(1264..1824)
                     /locus_tag="ACHWN2_002793"
     CDS             complement(1264..1824)
                     /locus_tag="ACHWN2_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR8932252.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(1950..2300)
                     /locus_tag="ACHWN2_002794"
                     /pseudo
     CDS             complement(1950..2300)
                     /locus_tag="ACHWN2_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(2503..3516)
                     /gene="intI1"
                     /locus_tag="ACHWN2_002795"
     CDS             complement(2503..3516)
                     /gene="intI1"
                     /locus_tag="ACHWN2_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR8932253.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3677..4150
                     /gene="dfrA17"
                     /locus_tag="ACHWN2_002796"
     CDS             3677..4150
                     /gene="dfrA17"
                     /locus_tag="ACHWN2_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR8932254.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            4281..5069
                     /locus_tag="ACHWN2_002797"
     CDS             4281..5069
                     /locus_tag="ACHWN2_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR8932255.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            5275..5622
                     /locus_tag="ACHWN2_002798"
     CDS             5275..5622
                     /locus_tag="ACHWN2_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR8932256.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            5616..6455
                     /gene="sul1"
                     /locus_tag="ACHWN2_002799"
     CDS             5616..6455
                     /gene="sul1"
                     /locus_tag="ACHWN2_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR8932257.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            6583..6786
                     /locus_tag="ACHWN2_002800"
     CDS             6583..6786
                     /locus_tag="ACHWN2_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932258.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            6942..8147
                     /gene="chrA"
                     /locus_tag="ACHWN2_002801"
     CDS             6942..8147
                     /gene="chrA"
                     /locus_tag="ACHWN2_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR8932259.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            8158..8463
                     /locus_tag="ACHWN2_002802"
     CDS             8158..8463
                     /locus_tag="ACHWN2_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR8932260.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            8690..9454
                     /locus_tag="ACHWN2_002803"
     CDS             8690..9454
                     /locus_tag="ACHWN2_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR8932261.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(9947..10531)
                     /gene="mphR(A)"
                     /locus_tag="ACHWN2_002804"
     CDS             complement(9947..10531)
                     /gene="mphR(A)"
                     /locus_tag="ACHWN2_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR8932262.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(10531..11769)
                     /gene="mrx(A)"
                     /locus_tag="ACHWN2_002805"
                     /gene_synonym="mrx"
     CDS             complement(10531..11769)
                     /gene="mrx(A)"
                     /locus_tag="ACHWN2_002805"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR8932263.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(11766..12671)
                     /locus_tag="ACHWN2_002806"
     CDS             complement(11766..12671)
                     /locus_tag="ACHWN2_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR8932264.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         2585 a         3701 c         3815 g         2638 t
ORIGIN      
        1 tgccgcgcag cacctcaatg gcatcaagca cgtttttggc ggcgggcgcg gcccgcagct
       61 tgagcacggc aaggaattcc ggtgcatagc ggcgcagggt ggcgtagctc tcgccgatgc
      121 gatgcaggaa atcgaagtca tcgggttgcg cgagcttctg cgcctcggtg acgctctcgg
      181 caaaggaatc ccaggacatg acggcctcga tggcggcaaa cgcatcgcgg cctgattgct
      241 tggcgtcgat cagcgcctga ccgatgcgcc cgtacagacg taccttggcg ttgatggcct
      301 tgcctgacgc ctggaactgc tgctgatgct tattcttggc agcgttaaac agcttaccca
      361 ggatgcggtc gtgcaggtcg atgatttcgt cggtgacggt ggccatgccc tcggtggcca
      421 gcgccacgag agtggcgtag cgccgttgcg gctcgaattt ggccaggtcg gcgggtgtca
      481 tctggccgcc ctcgcgggca atcttgagca ggcggttctg gtgaaccagc cgctcgatgc
      541 cggtaggcag atcgagtgcc tgccatgcct tgaggcgttc gatgtgttcc agcatatgcc
      601 gcgaatttgg cttggccgga gactggcgca accaagccaa ccaggtcgtc ttgccgttgt
      661 cccggcgctt gagcagatcg tcgaggcggc ggcgatgcgc gtccgccagt ggttcggcca
      721 aggcgtcgta gatgcgccgg ttagcacggg tgatcgcctc ggcactcgcc cgctcgacgg
      781 cgttgagggc gggcagaatg accgactgcc gccgcaggtg cccgatcaag gcgctggcca
      841 gcacgatgcc tttgtcggtt tgcatcgcca gctcggtcag catctggacg gcctgccggt
      901 aatggctcat ggtgaagggc cggaaaccga acacggtttg cagctcgctc aggtgctcgc
      961 gccgggtctg ctcccgctgg ccgtactcgt tccagctttc gacgccgacc ttgagctggt
     1021 cggcgaccag cttcaacaag ggcgggaacg gtagttcatc gacgcccagg atgacgccgg
     1081 gaaagcgcag gtaacagagc tgcaccgcga agcccagccg attggctggc ccgcgccgct
     1141 gtcggatgat cgagaggtcg gtatcgttga atgtgtaatg tcggatcagg tcgtccttgg
     1201 agtccggcaa cgccagcagg ctttcccgct cggcggcgga caggatggaa cgacgtggca
     1261 tatttactga tccgttctca agtattgata cagggtttcg cgactgattc cgaattcacg
     1321 agcaagcttg gtcttttgct cgccagcctc gacacgttgg cgcagttcgg caatacgctc
     1381 agacgacagg gatttcttcc tgccacggta agccccgcgt tgcttggcga gcgcaatacc
     1441 ctcgcgctga cgctcgcgga tcagggcgcg ctcgaactcg gcgaacgcgc ccatcaccga
     1501 gagcatcagg ttcgccatcg gagagtcttc gccagtaaaa ctgaggtgtt ccttgacgaa
     1561 ttcgatatgc acgccgcgtt gtgtcagcgt ttgcacgatc cggcgcaaat catcgagatt
     1621 gcgcgccagg cgatccatgc tatgcaccac cacggtgtcg ccggtgcggg cgaagcttat
     1681 cagcgcttcc agttgcggac gcttgacatc cttgccggat gccttgtcgc taaaagcgcg
     1741 atcaaccttg acgccttcca gttgccgttc cgggttctgg tcgaaggtgc tgaccctgat
     1801 atacccaatg cgctgtccag tcatggaatt ccctgcaaaa tgtcagggaa gactctatga
     1861 ccttcaacga gatatgtcaa taaattcaaa attcaatcct atcctgacgc aatttacaca
     1921 tggcatctga catcaggtta gggtatgcct caacctgacg gcggcgaatc acaagcgtcc
     1981 ggtttgacgc tggtttcggt cgcagtgctg gcccgcgcct ggaagcgctg atagcactcc
     2041 agcccgcaga aatgctccac gtactcggcc ccttccggcg tgaaggcggc atcgagcggg
     2101 atttccttgc agcacacgca gcaactggtg ctcggttcat tggcgttcat ggtggtgttc
     2161 ctccattggt tgacgaagcc gacgaaggcc gccgccggca tcggcctggc gaacaggaaa
     2221 ccctgcaccg tgtcgcaacc cgcctgccgc aaccacgcaa ggcagtcggg tgtttccaca
     2281 ccctcggcta ccacctccat tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
     2341 gactgcacta tagcagcgga ggggttggat ccatcaggca acgacgggct gctgccggcc
     2401 atcagcggac gcagggagga ctttccgcaa ccggccgttc gatgcggcac cgatggcctt
     2461 cgcgcagggg tagtgaatcc gccaggattg acttgcgctg ccctacctct cactagtgag
     2521 gggcggcagc gcatcaagcg gtgagcgcac tccggcaccg ccaactttca gcacatgcgt
     2581 gtaaatcatc gtcgtagaga cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc
     2641 gtaaccgctg cggagcaagg ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg
     2701 cttcgtgatg cctgcttgtt ctacggcacg tttgaaggcg cgctgaaagg tctggtcata
     2761 catgtgatgg cgacgcacga caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa
     2821 aacccagaac cacggccagg aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg
     2881 aagcgcaacg ccgctgcggc cctcggcctg gtccttcagc caccatgccc gtgcacgcga
     2941 cagctgctcg cgcaggctgg gtgccaagct ctcgggtaac atcaaggccc gatccttgga
     3001 gcccttgccc tcccgcacga tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag
     3061 ttgcaaaccc tcactgatcc gcatgcccgt tccatacaga agctgggcga acaaacgatg
     3121 ctcgccttcc agaaaaccga ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa
     3181 gcgccgcgac ggccgaggtc ttccgatctc ctgaagccag ggcagatccg tgcacagcac
     3241 cttgccgtag aagaacagca aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt
     3301 gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca aggttgccgg
     3361 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     3421 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     3481 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     3541 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     3601 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttagc
     3661 cattaaggga gttaaattga aaatatcatt gatttctgca gtgtcagaaa atggcgtaat
     3721 cggtagtggt cctgatatcc cgtggtcagt aaaaggtgag caactactct ttaaagcgct
     3781 cacatataat caatggctcc ttgtcggaag aaaaacattt gactctatgg gtgttcttcc
     3841 aaatcgcaaa tatgcagtag tgtcaaagaa cggaatttca agctcaaatg aaaacgtcct
     3901 agtttttcct tcaatagaaa atgctttgaa agagctatca aaagttacag atcatgtata
     3961 tgtctctggc gggggtcaaa tctataatag ccttattgaa aaagcagata taattcattt
     4021 gtctactgtt cacgttgaag tcgaaggtga tatcaaattc cctataatgc ctgagaattt
     4081 caatttggtt tttgaacagt tttttatgtc taatataaat tatacatacc agatttggaa
     4141 aaaaggctaa caatgcgttg cagcaccagt cgcttcgctc cttggacagc ttttaagtcg
     4201 cgtctttgtg gttttgctgc gcaaaagtat tccacaaagc cgcaacttaa aagctgccgc
     4261 tgaacttaac gttaggcatc atgggtgaat ttttccctgc acaagttttc aagcagctgt
     4321 cccacgctcg cgcggtgatc gagcgccatc tggctgcgac actggacaca atccacctgt
     4381 tcggatctgc gatcgatgga gggctgaagc cggacagcga catagacttg ctcgtgaccg
     4441 tcagcgccgc acctaacgat tcgctccggc aggcgctaat gctcgatttg ctgaaagtct
     4501 catcaccgcc aggcgatggc ggaacatggc gaccgctgga gctaactgtt gtcgctcgaa
     4561 gcgaagtagt gccttggcgc tatccggcgc ggcgtgagct tcagttcggt gagtggctcc
     4621 gccacgacat cctttccgga acgttcgagc ctgccgttct ggatcacgat cttgcgattt
     4681 tgctgaccaa ggcgaggcaa cacagccttg cgcttctagg cccatccgca gccacgtttt
     4741 tcgagccggt gccgaaggag catttctcca aggcgctttt cgacactatt gcccagtgga
     4801 atgcagagtc ggattggaag ggtgacgagc ggaacgtcgt tcttgctctt gctcgcattt
     4861 ggtacagcgc ttcaactggt ctcattgctc ctaaggacgt tgctgccgca tgggtatcgg
     4921 agcgtttgcc tgccgagcat cggcccctca tctgcaaggc acgcgcggcg tacctgggta
     4981 gcgaggacga cgacctagca atgcgcgtcg aagagacggc cgcgttcgtt cgatatgcca
     5041 aagcaacgat tgagagaatc ttgcgttgag cggcatgtgc gaaaagtgca tcgacccgcg
     5101 ccgagggcat ctgatgccta actcggcgtt caagcggacg ggctgcgccc gccgctcaac
     5161 tatgcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc
     5221 ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgaaa
     5281 ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacatc cgcattaaaa
     5341 tctagcgagg gctttactaa gcttgcccct tccgccgttg tcataatcgg ttatggcatc
     5401 gcattttatt ttctttctct ggttctgaaa tccatccctg tcggtgttgc ttatgcagtc
     5461 tggtcgggac tcggcgtcgt cataattaca gccattgcct ggttgcttca tgggcaaaag
     5521 cttgatgcgt ggggctttgt aggtatgggg ctcataattg ctgccttttt gctcgcccga
     5581 tccccatcgt ggaagtcgct gcggaggccg acgccatggt gacggtgttc ggcattctga
     5641 atctcaccga ggactccttc ttcgatgaga gccggcggct agaccccgcc ggcgctgtca
     5701 ccgcggcgat cgaaatgctg cgagtcggat cagacgtcgt ggatgtcgga ccggccgcca
     5761 gccatccgga cgcgaggcct gtatcgccgg ccgatgagat cagacgtatt gcgccgctct
     5821 tagacgccct gtccgatcag atgcaccgtg tttcaatcga cagcttccaa ccggaaaccc
     5881 agcgctatgc gctcaagcgc ggcgtgggct acctgaacga tatccaagga tttcctgacc
     5941 ctgcgctcta tcccgatatt gctgaggcgg actgcaggct ggtggttatg cactcagcgc
     6001 agcgggatgg catcgccacc cgcaccggtc accttcgacc cgaagacgcg ctcgacgaga
     6061 ttgtgcggtt cttcgaggcg cgggtttccg ccttgcgacg gagcggggtc gctgccgacc
     6121 ggctcatcct cgatccgggg atgggatttt tcttgagccc cgcaccggaa acatcgctgc
     6181 acgtgctgtc gaaccttcaa aagctgaagt cggcgttggg gcttccgcta ttggtctcgg
     6241 tgtcgcggaa atccttcttg ggcgccaccg ttggccttcc tgtaaaggat ctgggtccag
     6301 cgagccttgc ggcggaactt cacgcgatcg gcaatggcgc tgactacgtc cgcacccacg
     6361 cgcctggaga tctgcgaagc gcaatcacct tctcggaaac cctcgcgaaa tttcgcagtc
     6421 gcgacgccag agaccgaggg ttagatcatg cctagcattc accttccggc cgcccgctag
     6481 cggaccctgg tcaggttccg cgaaggtggg cgcagacatg ctgggctcgt caggatcaaa
     6541 ctgcactatg aggcggcggt tcataccgcg ccaggggagc gaatggacag cgaggagcct
     6601 ccgaacgttc gggtcgcctg ctcgggtgat atcgacgagg ttgtgcggct gatgcacgac
     6661 gctgcggcgt ggatgtccgc caagggaacg cccgcctggg acgtcgcgcg gatcgaccgg
     6721 acattcgcgg agaccttcgt cctgagatcc gagctcctag ggatcgcctc agaaaacgga
     6781 aaataaagca cgctaagccg taagtaagcg tgctcctgtg aaagccacag ctaaaactgc
     6841 gtagtacaca tagaggtatt tttttctaga tttatatcga atttcgttat cgaatttcgc
     6901 ttattattga tcaactcgtt gcagatcatg aaggcgaaag tatgacgaat aaccctaccg
     6961 atgacagcag accatggtcg gtctttctta tttttctgcg gcttggattg acatcttttg
     7021 gcggccccat tgcgcacttg ggctacttcc gcgccgaatt tgtcacacgg cggcgctggc
     7081 tctccgaacg gagctatgct gacttggtcg cgctttgtca gttcttgcca gggcctgcaa
     7141 gcagccaggt cggcatagcg gtaggactgt ctcgggctgg atacagcggg gcgctggctg
     7201 cttgggctgg cttcacgctg ccgtctgcca tagccttgat cctttttgcg ctcggcatct
     7261 ccagctatgg cgattacgtc tcgcagggcg cgttgcatgg cttaaaagtg gtggctgtgg
     7321 ccgtggtcgc tcaagcagta tggggcatgg cgcgtaacct atgcacggat gggctgcgag
     7381 tcaccatcat ggcaattgct acctgcgtcg ttttacttgt gccgtccgcg tggggacagg
     7441 ttggcgtgat tgctatcgca ggcatcgcag gccggttatt gttcaagcca gcgaaagttg
     7501 ttgagcatga ccccctacct atcacggtca gtcaccgggc cggcgtgctt tggctctcgc
     7561 tgttctttgt cttgctgatt ggcctgccgg tgttggccga actgatgcca agtcaaacca
     7621 tggcaatggt ggattccttc tatcgtgtcg gatcactggt gttcggcggt ggtcacgttg
     7681 tgctgccatt actgcaagcc gaagtggtgc cctccggctg ggtcaacaat gaatcctttc
     7741 tcgcggggta cggggcagct caagcggtgc ccggcccttt gttcacgttc gccgcgtttc
     7801 ttggtgcctc gatgaacacc gccccgtcgg gctggatcgg cggcattgtg tgtctgctgg
     7861 ctatcttcgc gccctcgttc ttgctggtcg tcggatcaat gccattttgg gagcgtttgc
     7921 gccgcaatac aggcatccaa gctgcgctgg ccgggatcaa tgccgctgta gtcggcttgc
     7981 tgctggccgc gctgtatcag cctgtatgga ctagcgccat ctttcagccg caagacttcg
     8041 gcttggcatt agttgccctt gtcgcactta tgttctggaa gctcccgccg tggctggttg
     8101 tcctcggtag cggtgcagct gggtggctgt tgagcgtcgc tctgtgaggc acaaaaaatg
     8161 actgacaaag acctctacgg cggtttgatc cgcctgcaca tccttcacca tgcagccgag
     8221 gaacctgtct ttgggctggg gatcatcgaa gagctacgcc gacacggcta cgagatgagc
     8281 gctggcaccg tgtacccgat gctgcacggc ctggaaaaaa aaggctatct gacctcacgc
     8341 cacgaacgca ccgggcgacg tgaacggcgt gtttatgaca tcactgaaca aggcagaact
     8401 gcacttgctg atgcgaaaac aaaggtcaag gagctgttcg gagagttggt agaaggtggt
     8461 tgagtttttg cttctatgtc aggttgagct ataccctaac tggattgtca ttttcagaag
     8521 acgactgcac cagttgattg ggcgtaatgg ctgttgtgca gccagctcct gacagttcaa
     8581 tatcagaagt gatctgcacc aatctcgact atgctcaata ctcgtgtggg ctctgttgca
     8641 aaaatcgtga agcttgagca tgcttggcgg agattggacg gacggaacga tgacggattt
     8701 caagtggcgc catttccagg gtgatgtgat cctgtgggcg gtgcgctggt attgtcgcta
     8761 tccgatcagc tatcgcgacc ttgaggaaat gctggcggaa cgcggcattt cggtcgacca
     8821 tacgacgatc tatcgctggg tccagtgcta cgccccggag atggagaagc ggctgcgctg
     8881 gttctggcgg cgtggctttg atccgagctg gcgcctggat gaaacctacg tcaaggtgcg
     8941 gggcaagtgg acctacctgt accgggcagt cgacaagcgg ggcgacacga tcgatttcta
     9001 cctgtcgccg acccgcagcg ccaaggcagc gaagcggttc ctgggcaagg ccctgcgagg
     9061 cctgaagcac tgggaaaagc ctgccacgct caataccgac aaagcgccga gctatggtgc
     9121 agcgatcacc gaattgaagc gcgaaggaaa gctggaccgg gagacggccc accggcaggt
     9181 gaagtatctc aataacgtga tcgaggccga tcacggaaag ctcaagatac tgatcaagcc
     9241 ggtgcgcggt ttcaaatcga tccccacggc ctatgccacg atcaagggat tcgaagtcat
     9301 gcgagccctg cgcaaaggac aggctcgccc ctggtgcctg cagcccggca tcaggggcga
     9361 ggtgcgcctt gtggagagag cttttggcat tgggccctcg gcgctgacgg aggccatggg
     9421 catgctcaac caccatttcg cagcagccgc ctgatcggcg cagagcgaca gcctacctct
     9481 gactgccgcc aatctttgca acagagcccg ccgtgctagt ctgctcggtg atggtggagt
     9541 gaagccaacc cgcaatcggg ttatgaatct gcatcgcgat tcgcaatcag ctgtctcttg
     9601 agcatgtcga actcctgcga tgttatctcg ccgtgctcgt gctgctacac caacttgtcc
     9661 aactcatccg ccacaccgct ccccaccacc tgcgatggcc aggttgtttg ctgggcgcat
     9721 tggcggagta tcgtctcgac ccgggacacc cacaccggca caaccttacg ggagtgattc
     9781 actgtcaaag aatcggcccg gtgctctgac gcaagtatcg ggatggtcac catttgtaag
     9841 ccgtagacct gagtggtgat caagacttcg ataccaccga ccgtaccggt actaatcgac
     9901 gacggtcgtg ttcgtcgcct gccgcaggga ctctgcacac ctccgtttac gcatgtgcct
     9961 ggaggagttg gaaatcgtcg tgttcgggaa acattaaaca caggatggca gcgatctgag
    10021 ccagcacatg atcagctagc tcaccatccg gatcgacggc ccactgcatc gtcgcgccag
    10081 cgatgaccga gtgcaggagc aactcagctg ccgcaggagc acctgggggc agtcgcttgc
    10141 ggatcccctc caccaccgcg cggttccgct ggatcgcaag cgtgcgtagc tccggcacct
    10201 ggagctcgta ccaggagatg agatagttca ccgagaagtc gttgcgagtg ttcatgctcc
    10261 gaacgagcac ctgcaaaaat tcccagagcc cttgcggccc tgcgcctatc ggtatcgcat
    10321 tcaggtaatg ccgcacctgc tcgacgccgc gctccatcat cctcaccagc agcgtatcgc
    10381 ggttggtgaa gcgctggatt aacgctgcgc gggagagccc cacctccttt gctactccgc
    10441 tgagcgtgaa ctctatggga ccgcaacgct tcagcactac ggtggcggcc tcgagtacct
    10501 cgtcatcgga cttgagcttg gggcggggca tcagtgttca ccttctgtat gggttggggc
    10561 ggaggctgtg gctgccgccg ccattgtagc aaattgaaga cggagcgaga gtagagccac
    10621 gagccccgca aacacggccg atacaacgag gccagggagc ggaccagcaa ggtcgacaaa
    10681 cgcgccggcc gcaagcataa ccatgggcga ggctgacagc atcaccgccg agaccgtgcc
    10741 gagtacccgg ccgagaagtt ctggcggcgt gcggttgtag atggcagcgt tgagaatggg
    10801 agagactgag ccggtcagca gtcccacgag cgcgcccaac aacatcagca ccggcacgcc
    10861 tggcaactgt gaaagcagaa gcgagcccac cgcagagcca caaaatgcca ccgccagcca
    10921 gttctgcgct gatatccggg cgccgaccga cgcatgaatg gcaatgccaa ggagaccacc
    10981 agcccccatc attgaggaga acagcccgag ctctgctact tggcgtcctg catctacaaa
    11041 cagcgcaggc atgatgacgc tgccgttggc gccaacgatg cccacgaaga tcatcactat
    11101 accaaagaga gggcgcagca ggggttcgct ccagagaaaa gcgacgccgg cgcgcatgga
    11161 gagagtcgcc gtcgtggtca tcgtccgagc ggcacgcgcg ggaagcaccc acgcgccgag
    11221 cagacctgca aggacggagc agaacgccgt cagcccgagc gttggcgcag cgccaagcag
    11281 gccgattgcg gcccccccaa gggccgggcc acctagaatc gcgacgttcc cgatcaccgc
    11341 tttcagtgac gagacgcgct caacggagag cccggcgacg tggccgagtt tgggcagctc
    11401 actgtcctgc gcggccatac cgggtgcgtc gaacgcggca ccgagcacca cgcaagcgat
    11461 cagcccagtg ttcgagaggg cgccaacggc atcgagcagt gggatgctcg ccatggccac
    11521 gccgcccacc acacccgaga tcaatgcgac gggcgcgcgc ccgaaccgat cgacgaggcc
    11581 accaccaacc cacgcgccga tgatggtcgc gatgacgctg ctagcggccg tggcgcccgc
    11641 ccaggccgcg ctctttgtat gagacaggac gaaccatgga agcgcgaggg ccgccaccgc
    11701 gttgccgatc cggaagagaa aggtcgccgc gaacagcgtc gcgagcgggc tatatcgacg
    11761 ttcgctcatt ccgctgcggc gagctgcgcc ttcgccgcag cgaggtactc ttcgttaccc
    11821 gagtcgaggg cgaagagtgc gtaggtgacc gccccgaacg caaggcgctc cgcgatgtgg
    11881 tgggcgagcc gcggccacac ccggccaccg gccgcttcat acgtgaggag gagcttcgcg
    11941 agcccctctt caccaaagac cataaggtgc gcggccatgt cgatggcagg gtcatcaacg
    12001 cgggcctcgc tccagtcgat catcccgctg acgcgctccg tgttgtcgat gagcacatgg
    12061 cccacgtaga gatcgccatg caccaccacg gagaaatctg gccacgacga atcgtcgtcg
    12121 agccagcgct gccaccggtg gaggcgcttg tcgttcacca cgaactcgcg tcggacgcgg
    12181 tcaacgtcgt cggccacctt ctgacgggcc tgcgtcggtg tacggatgag catccccgca
    12241 tccacggcgg cggaaatggg gacggcatgc agggcggcga gcgcggtcgc gaagctctcc
    12301 gcgaagacct ccgagtcctg cggcacgacc cagtcgggcg tggacgaacc aggctggatg
    12361 accatcgcag tcgagtcttc gagcatggga taggcaacga gctcggcgtt ggccacgcgc
    12421 cagtccggca ccgcgaacgg caggcgattc ttgagcattg ccagcacccg cgcctctggt
    12481 tcgaccttcg cgcttacctc ggctcggcgc gggatgcgca gcacccaccg acgtccatcg
    12541 tcgacggtgg cgatcacgat cctatagtcg agcccaagct cattgacagt cagcgggcca
    12601 tggagcttga gcccatgtcg ggctgcaagt gcgtacagtt gggaggtatc ggcggtcgtg
    12661 actacggtca tgattcactc ctgagggctt gacgggttta gccacctaaa tgtaacagtc
    12721 acgtcggtta tattcaatc
//
LOCUS       DAZSNO010000102        12733 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZSNO010000102 DAZSNO010000000
VERSION     DAZSNO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.102"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1038
                     /locus_tag="ACHWN2_002807"
     CDS             <1..1038
                     /locus_tag="ACHWN2_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR8932265.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1039..3204
                     /locus_tag="ACHWN2_002808"
     CDS             1039..3204
                     /locus_tag="ACHWN2_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR8932266.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKEADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3218..4129
                     /locus_tag="ACHWN2_002809"
     CDS             3218..4129
                     /locus_tag="ACHWN2_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFR8932267.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4129..5424
                     /locus_tag="ACHWN2_002810"
     CDS             4129..5424
                     /locus_tag="ACHWN2_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFR8932268.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5469..5699
                     /locus_tag="ACHWN2_002811"
     CDS             5469..5699
                     /locus_tag="ACHWN2_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932269.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5677..6177
                     /locus_tag="ACHWN2_002812"
     CDS             5677..6177
                     /locus_tag="ACHWN2_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR8932270.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6177..7595
                     /locus_tag="ACHWN2_002813"
     CDS             6177..7595
                     /locus_tag="ACHWN2_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR8932271.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7595..8443
                     /locus_tag="ACHWN2_002814"
     CDS             7595..8443
                     /locus_tag="ACHWN2_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR8932272.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8443..8898
                     /locus_tag="ACHWN2_002815"
     CDS             8443..8898
                     /locus_tag="ACHWN2_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR8932273.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8901..9593
                     /locus_tag="ACHWN2_002816"
     CDS             8901..9593
                     /locus_tag="ACHWN2_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR8932274.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9603..10934
                     /locus_tag="ACHWN2_002817"
     CDS             9603..10934
                     /locus_tag="ACHWN2_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR8932275.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10935..12665
                     /locus_tag="ACHWN2_002818"
     CDS             10935..12665
                     /locus_tag="ACHWN2_002818"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR8932276.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         3436 a         3144 c         3355 g         2798 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgcagact ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt
     1141 agccgagtgt cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga
     1201 caattcgatg ttgttcgccc ggaggtcagg aaactggtcg cagagatgcg ccggaaccct
     1261 gtcgacgttc tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg
     1321 gggatgtatc gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt
     1381 cgtgagcaga tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac
     1441 gatccaacaa gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac
     1501 gtcatatggg acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg
     1561 attaacgcct tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg
     1621 gataccctgc catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat
     1681 gatgtcgtct acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc
     1741 taccgcgacc cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc
     1801 atcgacgacc tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg
     1861 cgtgtgtata agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc
     1921 ggagagcata ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag
     1981 tgctacgagg gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg
     2041 tcgttcaacg ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag
     2101 caaatcgaag gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag
     2161 aacaggactg atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac
     2221 cctgaagtgc cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa
     2281 gtggctagtc ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc
     2341 aatcaactga acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc
     2401 gcaatgcgac gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct
     2461 cgtcatgtaa cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa
     2521 gttgtcgatt accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag
     2581 tgctatacag acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt
     2641 caggagttgc tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa
     2701 tacttcacgc tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa
     2761 ctggtgatga tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag
     2821 gcacaacaac agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg
     2881 caaggtcagg ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct
     2941 gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat
     3001 atggacctcg acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag
     3061 caacagcgca gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga agcagaccag
     3121 actcattcac aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc
     3181 ggagtagccg agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca
     3241 ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt
     3301 tgtcgataat gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tccggaaaga
     3361 cgatgagaaa ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga
     3421 acgcaaacgc cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc
     3481 ggagcacctg cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc
     3541 cgaagatgca ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca
     3601 ggcaaacagt gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca
     3661 gggtcgcaaa actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa
     3721 gcactacgag gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt
     3781 catgcaactg gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc
     3841 cgccgctctc atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat
     3901 ggacgggcaa tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc
     3961 tcgagccaag cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt
     4021 tgctgcaaat atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt
     4081 agagacgtac cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat
     4141 gaaggtcaac tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg
     4201 ccaatggcgt caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc
     4261 aacaccgtgt ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact
     4321 ggcaacgcaa ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac
     4381 gatttcttcg agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc
     4441 caggcatccg ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact
     4501 gaaatgggct cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct
     4561 ggctgggatt ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac
     4621 atgggcatca gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta
     4681 gatggtgaca tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt
     4741 cagattgctg gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca
     4801 accgcgactg gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt
     4861 gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc
     4921 accaccggat ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag
     4981 atggcgaaga atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt
     5041 actcacatcg aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa
     5101 gagaaggctt acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg
     5161 aacgtggcaa caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct
     5221 cagccgatcc cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt
     5281 ccaggtattg gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt
     5341 aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt
     5401 ggtctgccta accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg
     5461 agacaagcat gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg
     5521 gctacgactt tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt
     5581 tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc
     5641 gtaaaaagcc tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat
     5701 ttagttcttg cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc
     5761 gaaccgcagt ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt
     5821 ggcggtgatg cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct
     5881 ccggatccgg gcgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac
     5941 cttgcctgcc gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tataaccact
     6001 gccagatacg ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct
     6061 aaatgtaagt ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag
     6121 tggaatggtt ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc
     6181 ctattcagca acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata
     6241 tcgactatct gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat
     6301 atcttcgctc attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg
     6361 tcgagtacaa catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag
     6421 gagaaagtga agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga
     6481 catcacaggc ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca
     6541 aaaccgtctc aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc
     6601 gcgacattac gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt
     6661 ttatcactga ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg
     6721 agtctcagcc tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg
     6781 gttcatcgac tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt
     6841 atgttgcaca accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc
     6901 cattcgctga ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata
     6961 tcatcgactc cggtcaggtg tcaccaattg ccagcgcgag cattgagaaa atcctccgct
     7021 cctacactgc tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg
     7081 agttgctgat tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaatcgcca
     7141 atggcccaca atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg
     7201 acttcgttta cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga
     7261 aactgcaatt cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc
     7321 cactcttcaa agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg
     7381 ttgcgcagta cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc
     7441 gtgagcagat gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc
     7501 gagtagggcg catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg
     7561 tcactctgtc tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc
     7621 ggtaatcatc caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca
     7681 gtcgtatctg ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc
     7741 caacgaggcc ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat
     7801 tctcgctgac catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga
     7861 gcgaatcaga caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga
     7921 aagcgatatt tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca
     7981 tgaaacgcgc atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta
     8041 ctctggtgta tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac
     8101 gctcacaccg tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt
     8161 tgtgttcaac gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag
     8221 cggaaccgcc aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt
     8281 cagcagtcgt aatgcggcga caacaaccga caacatcctg ttagctacgt tcttcagcgt
     8341 ggataaagac ggctttcttg ccacaaatgg cagtacgtta accattcagt cgaatggtgc
     8401 ggcgtttact gccacaacca tcaagataat cgcggagcag tgatgattac attcaaacca
     8461 acgcgaaaca tcgacctgat agaagcagtc ggaaatcacc ctgacattat cgccgggagc
     8521 aacaacggtg atggatacga ctacaaacct gaatgccgtt actttgaggt gaacgtgcac
     8581 ggtcagtttg gcggcattgt ttactatcag gagattcagc cgctgacatt agattgccac
     8641 gccatgtacc tgccagagat tcgcggcttc agcaaggaaa ttgggctggc gttctggcga
     8701 tacattctga ctaacaccac cgttcagtgc gtaacatcgt tcgctgcacg caaattccga
     8761 cacggtcaga tgtactgcgc aatgattggc cttaagcgtg taggaaccat caagaaatac
     8821 ttcaaaggcg tggatgacgt gactttttac agcgccacac gcgaagaact aatcgacttc
     8881 ctgaatcacg ggagatagcc atgttatatg catttaagct gggcagaaaa ctgcgcggcg
     8941 aggaacctta ttgccctgaa aaaggcggga aaggtggcag ttctgataaa agcgcaaagt
     9001 atgcagcaga agcccagaag tatgctgcag acctgcaaaa tcagcagtgg cagacgatca
     9061 tgaaaaacct tgctccgttc acgcctcttg cggagcagta tgttaaccag cttcagaacc
     9121 tttccagttt agaaggtcag gggcaggcac ttaatcagta ttacaactct aagcagtata
     9181 aagaccttgc agggcaggcg cgttaccaga gtcttgctgc tgcggaggcg acgggtggac
     9241 ttggttcgac agccacaagc aatcaactgg ctacgatcgc gccgacactc ggtcagtctt
     9301 ggttatcaaa tcagatgagc aattacaaca atctggcaaa cgttgggctt ggtgcgctgc
     9361 aaggtcaggc aaacgccggg cagacgtacg ccaacaacat gagcagcatt gcacagcaaa
     9421 gcgcagctct tgccgctgct aatgccaata aaccatcaag tcttcagact gcaattagcg
     9481 gtggcacgtc tggtgcgatt gccggtgcag gtcttgccag ccttttggga acatcaacgc
     9541 cttggggcgc tggtattggt gctggtatcg gattgcttgg ctcgttgttt taagggggaa
     9601 tcatggctac ttggcaagga acaaacggcg gattgttggc tggtatcggc ggcgtcaact
     9661 caaacgctcc gagcgtaaat gacatcggca atacgcttca gcttatcagg cagaacaatg
     9721 atattgagcg ttcaggcgct aacaatgttg ggctgactgc tttgcaaggt ctttcaggta
     9781 ttgcgggtgt ttttcagcag gaaaagcagg ctcagcggca gaaagaattt cagcaggcgt
     9841 acgctaatgc ttatgcgtct ggtgatcgcg gtgctttgcg tcagttggct actcaatatc
     9901 cagaccagat tgaatccgtt cgtaaaggca tgggattcat tgatgaagag cagcgtaatt
     9961 ctatcggcac cttagcggct ggcgcacgcc ttgcgtcatc gtctccagaa gcaatgcaat
    10021 catggctgca aaacaacgcc ggtgagttag ctcgtgttgg cgttaatcct catgacgtcg
    10081 ctcagatgta ccaacagaac ccgcggcagt tcggcgaatt tgtcgatcac ctggggatga
    10141 acagtctcgg gcccgaaaaa tactttgacc tacaggataa aatgcagggt cgccaggtta
    10201 ccatgcgcgg tcaggatctg gattcgcaaa ccgccgctcg gaatcaggca atcacaatgc
    10261 gcggacaaga tatccaggcg aatttaggtc agcagcgcat taatctggac gcagaaacaa
    10321 accgcattaa caacgaaaat aagcgccttg accggatgct atcagcagaa actaacgacc
    10381 tgaagcgcca ggaaatacag agccgcatag cagccaacaa ccagcagttg cagcagaagc
    10441 agcaagcgct aaatgatggc tacaaagacg gcatcaacac cctcacaacc agcatgttca
    10501 ctctgaacga tatcgttagt tctccttcac ttaagagcat tacaggctta cgtggagtaa
    10561 tccccaacgt tccaggctca caggctgcag acactcaggc acgacttgat acctttaaat
    10621 cacaagcata cctgacagcg gttcaggcca tgcgaggcat gggcgcactt tctgatgccg
    10681 agggcaaaaa gctcgaccag gctgttggtt cgctgcagaa ctcgcagagc gaggagtcct
    10741 ttcgtcgcaa cgctggcgtc atcctgaaca cgctcaacca gaagcgtaat gaggcggttg
    10801 gtaagtacgt tcagcaaaac ggtatcaagc gagtggaagc gcctcaggct tctatagatt
    10861 acctgaagca gcaccccgag ctgtcaatcg acttcattaa tcgctacgga tatcttccat
    10921 ctttggggca gtaaatggct aattaccgtg atttgttaga gcaggctggc gcacgttacg
    10981 gtgtgccaga agggttgatg actgcactgg gtgccaagga gtcttcttac aaccctgccg
    11041 cagtaagctc cgccggggct gtaggattga ctcaggtcat gcctgggaca tggcgtgata
    11101 tgggttatac cgatgagcaa atgcaaaacc ccgaatatca ggctgacgct ggcgcgcgct
    11161 atctggcaaa gatgtaccag cagtttggta actggcgtga ctctcttcag gcttatcacg
    11221 acggtcccgg caacgttatg aaggcaaagc gtggtgaata tacgccagga cctgaaggcc
    11281 gcggttacgt tgatgatcgc tttgctcaat gggctggtga cccggtgaca gactcaacag
    11341 tcgaacagcg cgccacctct gcaaaggtac atcctcagca agaccctaac aacccgtttg
    11401 cacaactgga agtacagtca tccgaacaag tatcggcatc aggtgtgcag tcagacccaa
    11461 ataatccatt tgctcagatt gagcagcagg cagccagtca gcagccacct caacccgtaa
    11521 gttctgtcgc accgaaacct gttcagcaac aaacagttaa tcaggccaat aatgcaccaa
    11581 cacgggaaga accatcattg atgcaacaag ctggcgattg gctcactggt ggtcaaagtg
    11641 cagggcaaat tgcagaacag gctggtcgtg gtctggtaaa cataccattt gacgtattgc
    11701 agggtggcgc aagtctgatt aatgcaatca gtcaggggct tggggggcca aaagtattgg
    11761 atgatgttta tcgtccagta gacagaccga cagaccccta cgcgcaagct ggagagtcaa
    11821 taggcggtta tcttgttcct ggagcaggtg tagctggaaa catggtcatt ggttctctcg
    11881 ctgacgcggc gaatcaacgg ggtgattttg ccgaaaatgc cgctattaat gccggactta
    11941 gcattgctac gcatggcctg ataaatggcg ttacccgtgg tgttcgtggt gcatcaaata
    12001 taattagtgg caataaaaca tctgcacaga gagcgaccac tgcgccaaca gaaacatcac
    12061 cattctccgg tgatgccgct gcagcaacaa atcctgcggt tcatgccgca gaggcaagag
    12121 tagcacaagg tgtaccaatg acgcctgcga cgaagaaccc agaggaagtc gttcgcacag
    12181 tagcagcaca aaaaaggcca aatctcgctt catcgcttga tgaactagat atcaatcctc
    12241 aggctgaagt tctggagtct gctgaaaggc ttaatgttga ttcattactc ccttcacact
    12301 tttccgggaa cgagcaatac aaggcagttg agcaagcaat caagtcccgt gcgggttctg
    12361 ctctacaggt gcaggaaaat gaagcaatca ggcagctagc acagggcgcg ggtgagataa
    12421 ttgatcgcgt ttccggtgca aaagatgctc ttggtatgag cgacaagttt attgatacgg
    12481 tcaatggaag aatgtctgcg ctgatgaaac gaagcgacca gctttatcgc aatgttgaaa
    12541 aggcgatgcc tgcaggtgca aaaattgatg cgccatcaac aaggtcaatg ctcaaacagg
    12601 tggcagaaga tcttggcggg atgaaaaacc ttgaccctat tgaaaagaga gttgaggtag
    12661 cctgagttta acggacactc cttcctgaaa tagaatggca tcagaaggag ctaataatga
    12721 gcagaaaaac cca
//
LOCUS       DAZSNO010000103        12617 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZSNO010000103 DAZSNO010000000
VERSION     DAZSNO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12617
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.103"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(559..935)
                     /gene="rnpB"
                     /locus_tag="ACHWN2_002819"
     ncRNA           complement(559..935)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHWN2_002819"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(968..2113)
                     /gene="garK"
                     /locus_tag="ACHWN2_002820"
     CDS             complement(968..2113)
                     /gene="garK"
                     /locus_tag="ACHWN2_002820"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR8932277.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(2210..3100)
                     /gene="garR"
                     /locus_tag="ACHWN2_002821"
     CDS             complement(2210..3100)
                     /gene="garR"
                     /locus_tag="ACHWN2_002821"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR8932278.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(3130..3900)
                     /gene="garL"
                     /locus_tag="ACHWN2_002822"
                     /pseudo
     CDS             complement(3130..3900)
                     /gene="garL"
                     /locus_tag="ACHWN2_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3916..5250)
                     /gene="garP"
                     /locus_tag="ACHWN2_002823"
     CDS             complement(3916..5250)
                     /gene="garP"
                     /locus_tag="ACHWN2_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR8932279.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5625..7196
                     /gene="garD"
                     /locus_tag="ACHWN2_002824"
     CDS             5625..7196
                     /gene="garD"
                     /locus_tag="ACHWN2_002824"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR8932280.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(7248..8072)
                     /gene="agaR"
                     /locus_tag="ACHWN2_002825"
     CDS             complement(7248..8072)
                     /gene="agaR"
                     /locus_tag="ACHWN2_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR8932281.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            8321..9601
                     /gene="kbaZ"
                     /locus_tag="ACHWN2_002826"
     CDS             8321..9601
                     /gene="kbaZ"
                     /locus_tag="ACHWN2_002826"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR8932282.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9624..10097
                     /gene="agaV"
                     /locus_tag="ACHWN2_002827"
     CDS             9624..10097
                     /gene="agaV"
                     /locus_tag="ACHWN2_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR8932283.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10108..10887
                     /gene="agaW"
                     /locus_tag="ACHWN2_002828"
     CDS             10108..10887
                     /gene="agaW"
                     /locus_tag="ACHWN2_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR8932284.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10877..11686
                     /gene="agaE"
                     /locus_tag="ACHWN2_002829"
     CDS             10877..11686
                     /gene="agaE"
                     /locus_tag="ACHWN2_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR8932285.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11773..12207
                     /gene="agaF"
                     /locus_tag="ACHWN2_002830"
     CDS             11773..12207
                     /gene="agaF"
                     /locus_tag="ACHWN2_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR8932286.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12204..>12617
                     /locus_tag="ACHWN2_002831"
     CDS             12204..>12617
                     /locus_tag="ACHWN2_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR8932287.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         3041 a         3382 c         3188 g         3006 t
ORIGIN      
        1 agaatcaata ttttaccaga gaggactata cgtataatta cgacctttta aatgcaatat
       61 agacttaata acttcttaca aagtctatta ttaataccgt agatattcat cttaccctgt
      121 gcaccaaaag caaaaactta tcgacgcggg tttgaaactg atggcaaccg caaaaatgcc
      181 tgatgcgcta cgcttatcag gcctacgcca tctctgcaat atattgaatt tgcgtgcttt
      241 tgtaggcagg ataaggcgtt cacgcagcat ccggcatgaa taaagcgcac tcaacaatct
      301 gaaacccgcc ggaacggttt attacgtaca tcaggtgaaa ctgaccgata agccgctttc
      361 ttttgggtat agtgtcgtgg acagtcattc atctttctgc ccctccaaaa gcaaaaaccc
      421 gccgaagcgg gtttttacgt atatcaggtg aaactgaccg ataagccgct ttcttttggg
      481 tatagcgtcg tggacagtca ttcatctttc tgcccctcca aaagcaaaaa cccgccgaag
      541 cgggttttta cgtaaaacag gtgaaactga ccgataagcc gggttctgtc gtggacagtc
      601 attcatctag gccagcaatc gctcactggc tcaagcagcc tacccgggtt cagtacgggc
      661 cgtaccttat gaacccctat ttggccttgc tccgggtgga gtttaccgtg ccacggactg
      721 ttaccagccg cgcggtgcgc tcttaccgca ccctttcacc cttacctgat cccgcttgca
      781 cgggccatcg gcggtttgct ctctgttgca ctggtcgtgg gtttcccccc caggcgttac
      841 ctggcaccct gccctatgga gcccggactt tcctcccctc cacccgtctc ccccgaagag
      901 gacgacgacg aagcggcgac tgtctggtca gcttcggcgc gcagtataga gggtttgcgc
      961 gcccttgtca ccccgtgttg cgcattccaa tcgccagtgt cgcggcaata ttacgtgaag
     1021 cacggcagat attgtcataa gccccgcgga atgcttcgtc caacgtacct atgctggtca
     1081 atacgctgaa gaccgcatca atgccatgct gatgtacaac gccaacatca tcggtcaggc
     1141 tacccgcaat gccaatcacc ggtttatggt acttcttcgc cacgtttgcg acgccaatcg
     1201 gtaccttgcc gtgaatactc tggctgtcaa tacgcccttc accggtgatc accagcgtac
     1261 aatcgtgaat atgttcctcc agattcagcg ccgtagtgac gatttcaata ccacttttca
     1321 gttccgcacc aagaaacgcc atcagcgccg cacccatacc gcccgcagct cctgcgccgg
     1381 ggacatcttt tacatcaaca tgcagcgctt ttttaatgac atcggcatag tgagagaggt
     1441 tattgtccag ctcaacaatc atcgcttcac tggctccctt ttgtgggcca aagatgcgcg
     1501 atgcgccgtt atcgcccacc agcggattgg tgacatcaca agcgacgcga atgacgcaat
     1561 cttttaagcg cggatcgagg ccggaaatat caatatcatt cagagtatta agactaccgc
     1621 cgccaaaacc aatttcattg ccgttggcgt cgcataattt cgcccccagc gcctgtacca
     1681 tgcctgcgcc gccatcattt gtagcgctac cgccaatgcc gataataatg tttgtcgcgc
     1741 cgctctccag cgcctgcagg attaactcgc ctgtgccgcg tgaagtggtc acgagtggat
     1801 cgcgtttttc cgcaggtacc agctccagcc cactggccgc cgccatttca ataaacgcgg
     1861 ttttgccatc gccggagatc ccccaactgg cattcacttt ctcgcccagc ggccctgtaa
     1921 cccaggcgtg acgttcagaa ccctgggtgg ctgcaatcat cgcttccacc gttccttcgc
     1981 caccgtcggc aaccggaata gaaacgtact gtgcatcagg aaaaatttcc cgaaatcctt
     2041 tttctatcgc ctgcgcaacc tcgctggcag ataaactttc tttataagag tctggggcga
     2101 ttacgatttt catacctatg cctgttacca catgacgccg gagggcgttt ctcttattcg
     2161 gcctggattc caggcccgga ttgcaatacg ccatccgggc acgacgtcat taacgagtaa
     2221 cttcgacttt cgccagtttt tcgtagtagc acaccagggc gctatgatcc gccgttccta
     2281 aaccatctgc tcgcagtgcc tgcatcatct ccataaccgc agctgtgagc ggcagttgcg
     2341 cgccgacgcc gtgggaagta tccagcgcat ttgccagatc cttaatatgc agatcaatac
     2401 ggaagcccgg cttgaagttg cggtccatca ccatcggcgc tttggcatcc agcacggtac
     2461 tgcccgccag tccaccgcga attgcctgat acaccaggtc cgggttaacg cccgctttag
     2521 ttgccagcgt taacgcttct gacatcgcgg caatattcag cgccacaatg acctgattcg
     2581 ccagtttggt gacgttacct gcaccaattg ccccggtatg caccacggaa cccgccattg
     2641 ctttcatcaa atcatagtat ttgtcgaaaa tagccttgtc gccgcccacc atcactgaca
     2701 gcgtaccgtc gatggctttc ggttcaccgc cgctcaccgg agcatccagc atatcaatgc
     2761 ctttcgcttt cagcgcttcg ctgatttcac ggcttgccag cggtgcgata gaactcatat
     2821 cgatcaatac cgtacctggc ttcgcgcctt caataatgcc attctcaccc agcgccacct
     2881 ctttcacatg aggggagttt ggcagcatgg ttatgatgac gtcgcactgt tcagcgatcg
     2941 ctttagccgt agaggctgtt tctgcacctg cagcaatcac gtcagcaata gcttctgggt
     3001 tacggtcagc aaccaccagc gagtaacctg ctttcagaag gtttttactc attggtttac
     3061 ccataatccc caggccaata aaaccaactt tcatagtcat atcaatcatc tctcttgttg
     3121 cggtggtggt tattttttaa aggtatcagc cagtttatga gtggcagagc ggaagacgcc
     3181 gagatcgctg ccgacagcca caaacgtcgc gccccattcc agataacgac gcgcatcggc
     3241 ttcgaccggc gcgaggatac cgctgggttt gccgtgcgcg ctggcacggt taaaaatgtg
     3301 ctgaattgct ttttgtacat ccgggtgtga tgcattgccg agatggccta atgccgcggc
     3361 cagatcgctg gggccgacga agatgccgtc tacgccttcg gtagcggcaa tggcatcgac
     3421 gttatctacg ccctgctgac tttctatctg gaccagaata gtgatgttct tgttcgactg
     3481 agcgaaataa tccgccacgg tgccaaacat attggcgcgg tgagaaacgg agacgccgcg
     3541 aatgccttcc ggtgggtaac gggttgatgc caccgcctgc tctgcttcct cttttgtttc
     3601 tacaaaagga atcaggaagt tatagaaacc gatatccaga agacgcttaa taatttccgg
     3661 ctcgttggtc ggcactcaca ctactggcgc gctggcgctg cctttcaagg ccattaactg
     3721 cggaataaac gtggagatat cgtttggcgc atgttcgcca tccagcacca gccagtcaaa
     3781 cccagccaaa ccaagaactt cagtgctaat cgggttagag agtgctgacc agcaaccaat
     3841 ttgtacctgt ttcgcagcca gtgcggcttt gaatttattc gggaaaacat cgttattcat
     3901 cgcttatacc tttgcttatt tctgcaattc catacgttta atgtcgccaa ctacgaagag
     3961 gtagcagacc atcgccatca gcgctgaaca tcccacgaaa accagtgctg cattgaagga
     4021 gtgcagttca cttaccaggt agccaatcac cagtggagtg acaatggagg caacattgcc
     4081 aaagacgtta aagacgccgc cacagaggcc aacaatctct ttcggcgcgg tgtcagaaat
     4141 caccggccag cccagagcac caaatccttt gccaaaaaaa gccagcgcca tcagcatgac
     4201 caccagcgtg gtgttgttgg tgtagttaca taagatgatg gtggaagcca gcaacattcc
     4261 cagcacaatc ggtagcttac gtgccagggt cagggataaa ccgcgtttga tcagataatc
     4321 cgagaagaca cctcccagca cgccgcccgc aaaaccacac agtgccggaa tcgaggcgac
     4381 cagacccact ttcagaatcg acatgccttt ttcctgcacc agataaatcg ggaaccaggt
     4441 gaggaagaac caggtgatgg tgttgataaa atattgtccg aaaaatacgc ccaacatcat
     4501 gcggttagag agcaattgct tgatgtaatg cagtttggga ccgcttgctg ccgcgctgcc
     4561 cggctttttg tggtccatat cgaccaccgc gccattttca gagataaact tcagctcttc
     4621 ctcagacata cgtgggtgat ctgtcgggtt atgaatcaac ttgatccaca gcgccgtcag
     4681 cacaaaacaa atcaccccca taacggtaaa gacgtgctcc catccccagg cgaaagtcag
     4741 ccagccaagc agaggcgaaa agagcgccag cgagaaatat tgtgccgagt taaagatggc
     4801 ggaggcagta ccacgttctt tcgtcgggaa ccaggcggcg acaatacggg cgttcgccgg
     4861 gaaggatgga gcttccgaga atcccagcat aaagcgcata aagaacatgg agatccctgc
     4921 ccaagccagc gggaacatat caacaaagcc ttgcaggaag gtgaacagcg accagaaaaa
     4981 gaggctgtag gtgtaaactt ttttcgagcc aaacttatca agcaaccagc cgccggggat
     5041 ttgcatcagc aagtaggccc agccaaaagc ggagaagatg taacccatcg aaaccgcact
     5101 taactgcaac tcttttgcca cttcggtacc agcaatagac agcgttgcac gatcggcgta
     5161 gttaacggcg gtaacaataa aaataatcag taatattaaa tagcgggtat gcacaccttt
     5221 ccttttttcg tcaacggtgt ccagaatcat tttatttacc tcgggtactt atgctgattt
     5281 ttattattat ggggaaggtg ttatttataa gtttcattta tgccgtaacg acaatgaact
     5341 cgggaattag tataagcagc gcgagaataa taatcattgt gcaaatgcta atttaattaa
     5401 tactttttaa atattatttt gagcatatgc acataaggtt gcgcgctaaa gcacagattt
     5461 gcgctttacc ttgccgggcg gcactgcaat ccctaaaatg attgacattg atcacatttc
     5521 tgcgtttaaa ctcctgacat tcttatttca cccaatgaaa tcatttattt ttaaatgaga
     5581 ccaggtcctc attttaataa cccctggctg gagaatattg cacaatggcc aacatcgaaa
     5641 tcagacaaga aacgccaact gcgttttata taaaagttca cgacacagat aatgtggcaa
     5701 ttattgttaa tgataatggc ctgaaagcag gaacgcgttt tccggatggg ctggaattaa
     5761 ttgaacatat tccccagggg cataaagtcg cattgctgga cattccggct aatggtgaaa
     5821 ttattcgtta tggcgaagtg attggttacg ccgtgcgtgc aatcccacgc ggaagctgga
     5881 tcgacgaatc aatggttgta ctaccggaag cgccgccgtt acacacgctg ccactggcaa
     5941 ccaaagtccc ggaaccctta ccgccgctgg aaggatacac ctttgagggc tatcgcaatg
     6001 ccgatggcag cgtgggcacc aaaaacctgc tcggcatcac caccagcgtc cactgtgtgg
     6061 caggtgtggt ggactatgta gtaaaaatca ttgaacgcga tctgctaccg aaatacccga
     6121 acgtcgatgg cgtggtgggg ctgaatcatt tgtacggttg tggcgtggcg attaacgcac
     6181 cggcagcagt tgtacctatt cgtaccattc acaatatttc gctgaaccct aactttggcg
     6241 gcgaagtaat ggtgattggc ctgggttgtg aaaagttgca gcctgagcgc ctgctgactg
     6301 gaacggatga tgtgcaagct attccagtag aaagcgccag cattgtcagt ttgcaggatg
     6361 aaaagcatgt cggttttcag tccatggtcg aggatatttt gcaggtcgcc gaacgccatc
     6421 tacaaaaact gaatcaacgt cagcgagaaa cctgtccggc ttcagaactg gttgtcggca
     6481 tgcattgcgg tggcagcgat gcgttttctg gcgtaacggc aaacccggcg gttggctatg
     6541 cgtctgatct actggtgcgc tgcggcgcaa cggtgatgtt ttcagaagtc acggaagtgc
     6601 gtgacgcgat ccatctgctg acaccacgcg cagtgaacga agaggtcggc aaacggctgc
     6661 tggaggagat ggagtggtac gataactatc tcaatatggg aaaaaccgac cgcagcgcca
     6721 acccttcgcc gggcaacaag aaaggcggtc tggcaaacgt ggtagagaaa gcgctcggct
     6781 ccattgctaa atcgggcaaa agtgcaattg ttgaagtgct gtcgcccggt caacgcccga
     6841 ctaaacgcgg attaatttac gccgcgacgc cagccagcga ttttgtctgt ggcacgcaac
     6901 aggtggcttc gggtatcacc gtgcaagtgt ttacgaccgg tcgtggtacg ccgtacggcc
     6961 tgatggcggt acccgtcatt aaaatggcac cccgcaccga gctggcgaac cgctggtttg
     7021 atttaatgga tattaatgcg ggcaccatcg ctaccggcga agaaactatt gaagaggagg
     7081 gctggaagtt gttccacttt attctcgacg tcgccagcgg gaagaagaaa accttctcgg
     7141 atcaatgggg actgcataac cagctggcgg tgtttaaccc agcaccggtg acctgataat
     7201 tcaattcgta ttatgcccga tagcacacgt ttatcgggca tattgagtca cagcgaagac
     7261 gcgtgatccc cgaccagaat cacttcaacc ccagcctttc gcagtccttc cagactatcc
     7321 gcaggaatgc cttcatcaac aatgatcatg tcgatacgtt gagtatcaat gatcttatgt
     7381 aaactggaac gattgaattt actggaatcg gtgaccacga tgatccgttc cgcaacttcg
     7441 cacatccgac ggcttaaacg ggcttcatct tcattatgtg tgctgacgcc gcgctccaga
     7501 tcgatcgcat ctacaccaag aaacagcata tcgaagtggt aattttgcag cgattgctca
     7561 gcctgatcgc cgtaaaaaga ttgcgactga cggcgcaaat gcccgccggt catcagcagc
     7621 tcaacgcctt ccgcttccag caacgcatta gccacgttca taccgttggt catcgcaatt
     7681 acgtcagtgt acttgcgcat cagacgagca atctcaaaag tggtggtccc ggaatcgagg
     7741 atcacccgat gacctggctg aatcaactca acggcagctt tcgcaacgct gcgtttcatc
     7801 gcggtgttca gtgcgctttt atcttccact gatggctcga ctgacggcgt cgtgctatcg
     7861 cagatcaacg cgccaccata ggcacgcaca gcgatcccct gcttttccag aaacgccaga
     7921 tcattgcgga tcgtcacagt agatacgcca tacaatgccg acagatcgtt aacctgcaca
     7981 ctcccttgct gtcgcagacg ctgaatgatc tgttctcgtc gctcgctggt gcctgtcact
     8041 cgcttctcac ctgaagcgtc ggtattactc atagtaagtc ctttcgtaaa actttcgttt
     8101 catttcgttt tgcctattaa cgcctttcta ttaagcaaat gcaagcccac cttgcccatt
     8161 ggcgcaagct actctcgttt cagtgacttt cattacgttt cttttgtgaa tcagatcaga
     8221 aaactattat ctttcgtttt atttttatct caccatgacg cagtatcaat tgaaacaaaa
     8281 cgaaagatca atatcgcagt aatctgaact ggagaggaaa gtgaaacatc tgacagaaat
     8341 ggtgagacag cacaaagcgg gcaaaacaaa tgcaatttat gccgtttgtt ccgcacatcc
     8401 gctggtgctg gaggctgcaa tccgctacgc cagtgcaaac caaacgccgt tactgattga
     8461 agcaacctcc aatcaggtag accagttcgg cggttatacc ggaatgacgc ccgccgattt
     8521 tcgcggcttt gtttgtcagc tcgccgactc gttgaatttc ccgcaggatg cgctgattct
     8581 gggtggtgac catctggggc caaaccgctg gcaaaacctg ccagccgctc aggcaatggc
     8641 caatgccgat gatttgatta aaagctacgt tgcggcagga ttcaaaaaaa tccaccttga
     8701 ttgcagcatg tcctgtcagg acgatccaat tcccttaact gatgacatcg tggctgaacg
     8761 cgccgcccgt ctggcgaaag tggcggaaga aacctgtcgc gaacactttg gcgaagccga
     8821 tctggagtat gtcattggta ccgaagtgcc ggtacctggc ggcgcgcatg aaaccttaag
     8881 cgagctggcg gtcaccacgc cggatgccgc ccgcgccacg ctggaagccc atcgtcacgc
     8941 ctttgaaaag caaggtttga atgccatctg gccacgcatt attgccctgg tggttcaacc
     9001 cggcgtcgaa ttcgatcaca ccaacgttat tgattatcag cccgccaaag cgagcgcctt
     9061 aagccagatg gtcgaaaact acgaaacgct gattttcgaa gcgcactcta ccgattatca
     9121 aacgccgcaa tctctgcgcc agctggtgat tgaccacttt gccattctga aagttggccc
     9181 ggcgctgacc ttcgccctgc gtgaagccct tttttctctg gcggcgattg aagaagaact
     9241 ggtaccagcg aaagcctgtt ccggtctgcg tcaggtgctg gaaaacgtga tgctcgaccg
     9301 cccggaatac tggcaaagcc actaccacgg tgacggcaac gcgcgtcgtc tggcgcgtgg
     9361 ttacagctac tcagatcgcg tgcgctatta ctggccggac agccagattg atgacgcttt
     9421 cgctcatctg gttcgtaatc tggcggattc accaattccg ctaccgctca tcagccagta
     9481 tctgccgctg cagtacgtga aagttcgctc cggcgagctg caaccgacgc cacgggaact
     9541 cattataaac catattcagg acatcctggc gcagtaccac acagcctgtg aaggccaata
     9601 agcaaaacaa agaggaacat gctatgccaa atattgtttt aagccggatt gatgaacgct
     9661 tgattcacgg tcaggtcggc gttcagtggg tcggatttgc gggggcaaat ctggtgctgg
     9721 tagccaacga tgaggttgcc gaagatccgg tacaacaaaa cctgatggaa atggtactgg
     9781 cagaagggat cgccgtacgt ttctggacgc tgcaaaaagt tatcgacaac attcatcgcg
     9841 ccgccgatcg acagaaaatc ctgctggttt gtaaaacacc cgccgatttc ctgacgctgg
     9901 tgaaaggtgg cgttccggtg aatcgcatta acgttggcaa tatgcactac gccaatggca
     9961 aacaacaaat cgccaaaacg gtttctgtgg atgcgggcga tatcgcagca tttaacgacc
    10021 tgaaagccgc tggggtggaa tgcttcgttc agggcgtccc gacagagcct gctgtggacc
    10081 tctttaaatt actttgaggg attcatcatg gaaatcagcc tgttgcaggc atttgcgttg
    10141 ggcattatcg cctttatcgc tggcctagat atgtttaacg gcttaaccca tatgcaccgc
    10201 ccggtggtcc tcggcccgtt ggtcgggctg gtacttggcg atctgcatac cggaatttta
    10261 accggcgata cgctggaact agtgtggatg gggctggccc cgctggcggg cgcacagccg
    10321 cctaacgtga ttatcggtac tatcgtcggc acggcgtttg ccattactac tggcgtgaaa
    10381 cccgatgtcg cggtcggtgt cgccgtacct ttcgctgtcg cagtacagat ggggattacc
    10441 ttcctgttct cggtgatgtc tggcgtgatg tctcgctgcg accggatggc ggagaacgcc
    10501 gatactcgcg gcattgaacg cgtgaactat ctggcactgc tggcactcgg cactttctat
    10561 tttctctgcg ctttcctgcc tatctacttc ggcgcggaac atgcaaaaac catcattgat
    10621 gtcctgccgc aacgattaat cgacggcctc ggcgtcgcgg gcggcatcat gccagcaatc
    10681 ggttttgccg tgctgctgaa aatcatgatg aaaaacgtct acatacccta cttcatcctg
    10741 ggctttgttg ccgcaacctg gctcaagtta ccggtgctgg ctattgctgc cgccgcgctg
    10801 gcgatggcgc tgatcgatct gctgcgtaaa tctcctgaac cgactcaacc tgcggcacag
    10861 aaagaggaat ttgaagatgg catctaatca aaccaccctg ccgaacgtca gcgaaaatga
    10921 agaaacactg ctgactggcg tcaatgaaaa cgtgtatgaa gatcggagca ttgccgcgga
    10981 gctgacgaaa aaagatatca accgcgtcgc ctggcgttcc atgctgttac aagcgtcttt
    11041 taactacgaa cgtatgcagg cttctggctg gttgtacggt ctgctgcctg ctctgaaaaa
    11101 gatccacact aataaacgcg acctggcgcg cgccatgaag gggcatatgg gtttcttcaa
    11161 tacccatccg tttctggtga cgtttgttat cggcattatc cttgcgatgg agcgttctaa
    11221 gcaggacgtt aacagtattc agagcaccaa aattgccgtc ggtgcgccgc ttggcgggat
    11281 tggcgatgcg atgttctggc taacgctact gccgatttgt ggcgggatag gcgcaagcct
    11341 cgcgctacaa ggctctattc ttggcgcagt cgtctttatt gtgctgttca acgtggtaca
    11401 cttggggctg cgttttggtc tggcgcatta tgcttaccgc atgggcgtgg cggcgattcc
    11461 ccttattaaa gcgaatacca aaaaagtcgg ccatgctgcg tcaatcgttg gaatgacggt
    11521 aatcggcgca ctggtggcaa cctatgttcg tttaagcacc acgctggaaa tcacggcggg
    11581 cgacgcagta gttaagttac aggctgatgt tatcgacaaa ctgatgcctg ccttcttacc
    11641 gctggtctac accctgacca tgttctggct ggtacgccgc ggctagagtc cgctgcgcct
    11701 gattgcggtt accgtggttc tagggatcgt cggtaaattc tgccatttcc tttaaataca
    11761 atgaggtatt agatgttaag tattattttg acagggcatg gcgggtttgc cagtggcatg
    11821 gaaaaagcaa tgaagcaaat cctcggcgat caatcgcagt ttattgccat cgattttccc
    11881 gaaacctcaa gcgccgcgct gcttacatcg cagcttgaag aggccatcgc ccaactggat
    11941 tgtgaagacg gcattgtttt tctgacggat ttgctgggcg gcacgccgtt tcgcgtagct
    12001 tcaacgctgg cgatgcaaaa accgggctgt gaagtgatta ccggcaccaa tttgcaactg
    12061 ctactggaga tggtgctgga gcgcgaaggg ttaagcggtg aagaattccg tgtacaggcg
    12121 ctggagtgtg gacaccgagg gctgaccagt ctggtggacg agttaggccg ctgccatgaa
    12181 gaatgtccgg tcgaggaagg aatatgacac acgttctgcg cgccagaagg ctgctgactg
    12241 aagagggatg gttcgatgac catcagttgc gtgttgctga cggtgtcatc gcagcaatcg
    12301 aaccgattcc agtgggcgtg actgaacgcg atgcggaacg gctctgcccc gcttacatcg
    12361 acacccatgt acacggtggt gcgggcgttg atgtaatgga tgacgcgccg gatgtactcg
    12421 acaagctggc aatgcacaag gcacgcgaag gtgtcggcag ttggttaccg accaccgtaa
    12481 ccgcgccgct taataccatt catgcggcgc tgaaacgtat tgctcaacgt tgccaacgcg
    12541 gcggacctgg tgcgcaagtg ctggggagtt atctcgaagg accgtacttc acgccgcaga
    12601 ataaaggcgc gcatccg
//
LOCUS       DAZSNO010000104        12482 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZSNO010000104 DAZSNO010000000
VERSION     DAZSNO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12482
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.104"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            4..1518
                     /gene="caiT"
                     /locus_tag="ACHWN2_002832"
     CDS             4..1518
                     /gene="caiT"
                     /locus_tag="ACHWN2_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR8932288.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1549..2691
                     /gene="caiA"
                     /locus_tag="ACHWN2_002833"
     CDS             1549..2691
                     /gene="caiA"
                     /locus_tag="ACHWN2_002833"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR8932289.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2820..4037
                     /gene="caiB"
                     /locus_tag="ACHWN2_002834"
     CDS             2820..4037
                     /gene="caiB"
                     /locus_tag="ACHWN2_002834"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFR8932290.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4111..5664
                     /gene="caiC"
                     /locus_tag="ACHWN2_002835"
     CDS             4111..5664
                     /gene="caiC"
                     /locus_tag="ACHWN2_002835"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR8932291.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5773..6558
                     /gene="caiD"
                     /locus_tag="ACHWN2_002836"
     CDS             5773..6558
                     /gene="caiD"
                     /locus_tag="ACHWN2_002836"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR8932292.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6564..7154
                     /gene="caiE"
                     /locus_tag="ACHWN2_002837"
     CDS             6564..7154
                     /gene="caiE"
                     /locus_tag="ACHWN2_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR8932293.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7240..7635)
                     /gene="caiF"
                     /locus_tag="ACHWN2_002838"
     CDS             complement(7240..7635)
                     /gene="caiF"
                     /locus_tag="ACHWN2_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR8932294.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMCR"
     gene            complement(7897..11118)
                     /gene="carB"
                     /locus_tag="ACHWN2_002839"
     CDS             complement(7897..11118)
                     /gene="carB"
                     /locus_tag="ACHWN2_002839"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR8932295.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11136..12284)
                     /gene="carA"
                     /locus_tag="ACHWN2_002840"
     CDS             complement(11136..12284)
                     /gene="carA"
                     /locus_tag="ACHWN2_002840"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR8932296.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
BASE COUNT         2909 a         3258 c         3308 g         3007 t
ORIGIN      
        1 ctcatgaaga atgaaaagag aaaaacggga atagaaccga aggttttctt tccgccatta
       61 ataatcgtcg gcatactttg ttggcttaca gtcagagatc tggatgcggc gaatgtcgtt
      121 attaatgctg tattcagtta cgtcaccaat gtatggggat gggcatttga atggtatatg
      181 gtggtgatgc ttttcggttg gttctggctg gtgtttggcc cgtatgccaa aaagcgttta
      241 ggtaacgaac cgccagaatt tagcaccgcc agttggatct ttatgatgtt cgcctcctgt
      301 acgtctgctg ccgtactgtt ctggggatcg attgagatct actactacat ctccaccccg
      361 ccgtttggct tagaaccgaa ctcgacagga gcgaaagagt tggggctggc ttacagcttg
      421 ttccactggg gacctctgcc gtgggccact tacagcttcc tttcagtcgc cttcgcttac
      481 ttcttctttg tccgcaaaat ggaagtgatt cgccccagct cgacactggt gccgctggca
      541 ggtgaaaaac acgccaaagg gttgttcggt accatcgtcg acaacttcta tctcgtcgcc
      601 ttgatcttcg cgatgggtac cagtctgggc cttgccacgc cgctggtgac cgagtgtatg
      661 caatggttgt ttggcattcc gcataccctg caactggacg ctatcatcat tacctgctgg
      721 attatcctca acgccatttg cgtcgcttgc ggtctgcaaa aaggggtacg tatcgccagt
      781 gacgtgcgta gttacctgag cttcctgatg ctgggttggg tgttcatcgt cagcggtgcc
      841 agcttcatca tgaactactt caccgattcg gtggggatgt tgctgatgta tctgccgcgc
      901 atgttgttct ataccgatcc catcgctaaa ggcggcttcc cgcagggctg gaccgtgttc
      961 tactgggcat ggtgggtgat ttacgccatc cagatgagta tcttcctcgc ccgcatctcc
     1021 cgtggtcgta ctgtgcgtga actgtgcttc ggcatggtga tggggctgac agcatccacc
     1081 tggatcctgt ggaccgtgct cggtagtaac actctgctgt tgatggataa aaacatcatc
     1141 aacattccaa atctgatcga acagtacggt gtggcgcgcg ccatcatcga aacctgggcc
     1201 gctctgccac tcagcaccgc caccatgtgg ggcttcttca tcctctgctt tattgccacc
     1261 gttacgctgg ttaacgcctg ctcttatacc ctggcgatgt ctacttgccg cgaagtacgc
     1321 gatggtgaag aaccaccgct gctggtgcgt atcggttggt caattctggt tggcattatc
     1381 ggtattgttc tgctggcgct cggcggcctg aaaccgattc aaaccgccat tatcgccgga
     1441 ggatgcccgc tgttcttcgt caacattatg gtgacgctct cctttattaa agacgcgaaa
     1501 cagaactgga aagattaatt aacccccaaa atatcaagag gttgaaagat ggattttaat
     1561 ttaaatgatg agcaggaact gtttgtcgcc ggtatccgcg aactgatggc cagcgaaaac
     1621 tgggaggcct attttgccga gtgcgaccgt gacagcgtct acccggaacg ttttgtcaaa
     1681 gcactggcgg atatgggtat cgacagtctg ctgatccctg aagagcacgg tggtctggac
     1741 gcggggtttg ttactctcgc cgccgtgtgg atggagctgg gacgtctggg ggcaccaacc
     1801 tatgtactgt accagctgcc gggcgggttc aataccttcc tgcgcgaagg cacacaagag
     1861 cagatcgaca agattatggc tttccgcggc accggtaagc agatgtggaa ctcagcgatt
     1921 accgaaccgg gcgcgggctc cgacgtgggt agcctgaaaa cgacttatac ccgtagaaat
     1981 ggtaagattt atcttaatgg tagtaagtgt tttattacca gtagcgccta caccccgtac
     2041 atcgtggtga tggcgcgcga cggggcttct ccggacaaac ctgtctacac cgaatggttt
     2101 gttgatatga gcaaaccggg catcaaagtg accaaacttg agaagctcgg tctgcgtatg
     2161 gatagctgct gtgaaatcac ctttgacgat gtggaactgg acgagaaaga catgttcggt
     2221 cgggaaggta acggctttaa ccgcgtcaaa gaagagttcg accatgaacg tttcctggta
     2281 gccctgacca actacggtac ggcgatgtgc gcctttgaag atgcggcgcg ctacgccaac
     2341 cagcgcgtgc agtttggcga ggctattggt cgtttccagt tgattcagga aaaattcgcc
     2401 cacatggcga tcaaattaaa ctccatgaaa aacatgctgt atgaagcagc gtggaaagca
     2461 gacaacggca ccatcacctc tggcgatgca gcgatgtgca aatacttctg cgccaatgcg
     2521 gcatttgaag tggtggatag cgcaatgcag gtgctgggcg gtgtcgggat tgcgggcaac
     2581 caccgcatca gccgcttctg gcgtgacctg cgtgtagacc gcgtttccgg aggatctgac
     2641 gaaatgcaga tcctgacgct gggtcgtgcg gtgctgaagc aataccgcta agtccgtgcg
     2701 ctgcctgatg cgacgctgac gcgtcttatc aggcctacgg gagcacgaat gtaagccgga
     2761 taaggcgttt acgccgcatc cggcagtcat gcgctacata aatttttcag gagctaataa
     2821 tggatcatct acccatgccg aaattcggac cgctggccgg attgcgcgtt gtcttctccg
     2881 gtatcgaaat cgccggaccg tttgccggac aaatgttcgc agaatggggc gcggaagtta
     2941 tttggatcga gaacgtcgcc tgggccgaca ccattcgcgt tcaaccgaac tacccgcagc
     3001 tctcccgccg caatttgcac gcgctgtcgt taaatatttt caaagatgaa ggccgcgaag
     3061 cgtttctgaa attaatggaa accaccgata tcttcatcga agccagtaaa ggtccggcct
     3121 ttgcccgtcg tggcattacc gatgaagtac tgtggcagca caacccgaaa ctggttatcg
     3181 ctcacctgtc cggttttggt cagtacggca ccgaggagta caccaatctt ccggcctata
     3241 acaccatcgc ccaggccttc agtggttacc tgattcagaa cggtgatgtt gaccagccaa
     3301 tgcctgcctt cccgtatacc gccgattact tttctggcct gaccgccacc acggcggcgc
     3361 tggcagcact gcataaagtg cgtgaaaccg gtaaaggcga aagtatcgac atcgccatgt
     3421 atgaagtgat gctgcgtatg ggccagtact tcatgatgga ttacttcaac ggcggcgaaa
     3481 tgtgcccgcg catgagcaaa ggtaaagatc cctactacgc cggttgcggt ctgtataaat
     3541 gtgccgacgg ctacatcgtg atggaactgg tgggcattac ccaaattgaa gagtgcttta
     3601 aagatattgg cctcgcacat ctactcggta cgccggaaat cccggaaggc actcagctta
     3661 tccaccgtat cgaatgccct tacggcccac tggtagaaga aaaactcgat gcctggctgg
     3721 cggcacatac catcgcagaa gttaaagaac gctttgccga actgaatatc gcctgtgcca
     3781 aagtactgac cgtaccggaa ctggaaagca atccacagta tgttgcccgt gaatcaatca
     3841 ctcagtggca aacgatggat ggtcgcacct gcaaagggcc gaacatcatg ccgaaattca
     3901 aaaataaccc cggacaaatc tggcgcggaa tgccctcaca tggcatggac acggctgcca
     3961 ttttgaaaaa tatcggctac agcgaaaacg acattcagga gttggtcagc aaaggtctgg
     4021 ccaaagttga ggactaaacg cgtggcgtca gtcggcttgc ctgactagcg cccgcccaat
     4081 aacaaacgaa caaaaatgga tagaggtgca atggatatca ttggcggaca acatctacgt
     4141 caaatgtggg acgatctggc ggacgtttac ggtcataaaa cggcgctgat ttgtgaatcc
     4201 agcggcggag tcgttaaccg gtatagttat cttgagttaa atcaggagat taaccgcacg
     4261 gcaaacctgt tttatacgct ggggattcgc aaaggcaaca aggttgcact acatctcgac
     4321 aactgcccgg aatttatctt ttgctggttc gggctggcaa aaattggcgc gattatggtg
     4381 ccgattaacg cccgcctgtt acgcgaggaa agcgagtgga tcttgcaaaa tagccaggcg
     4441 tgcctgctgg tgaccagtgc gcaattttat cctatgtatc aacagattca gcaggaagat
     4501 gccactcaat tgcggcacat ttgcctgaca gatgtggcac ttcccgctga tgatggagtg
     4561 agttcattta ctcaactgaa aaatcaacaa cctgccacct tgtgctatgc accgccgcta
     4621 tcgactgacg atacggcgga aattctcttc acctccggca ccacctcccg accgaaaggt
     4681 gtggtgatta cccattacaa cctgcgcttc gctggatatt actccgcctg gcagtgtgca
     4741 ctgcgtgacg atgacgtcta catgacggta atgcctgcgt ttcatatcga ttgccagtgt
     4801 actgcggcga tggcggcgtt ttctgccggg gccacctttg tgctggtcga gaaatacagc
     4861 gcccgcgcct tctggggaca ggcgcagaag taccgcgcca ccattaccga atgtattccg
     4921 atgatgatcc gtacgttgat ggtgcagccg ccttcagcga acgatcggca acaccgcctg
     4981 cgggaagtga tgttttatct caacttgtcg gagcaggaaa aagacgcatt ttgtgaacgc
     5041 ttcggcgttc gcttgctgac gtcttatggg atgacggaaa ccattgtggg cattatcggc
     5101 gatcgcccta gcgataaacg acgctggccg tcgattggtc gggcggggtt ttgctacgaa
     5161 gcggagatcc gcgacgatca caatcgcccg ctcccggcag gtgagatcgg tgaaatctgt
     5221 attaaaggcg taccagggaa aaccatcttc aaagagtatt ttctcaaccc gaaagccact
     5281 gcgaaagtgc tggaagccga cggctggcta cataccggcg ataccggata ccgcgacgaa
     5341 gaaggctttt tttatttcgt cgatcgccgc tgcaatatga tcaaacgcgg tggcgagaat
     5401 gtctcctgcg tggagctgga aaatatcatc gccacccatc cgaaaattca ggacatcgtg
     5461 gttgtgggta ttaaagattc gattcgcgat gaagccatca aagcatttgt ggtgctgaat
     5521 gaaggtgaaa cattgagcga agaggaattt tttcgcttct gcgaacaaaa tatggcgaaa
     5581 tttaaagtgc cctcttatct ggagatcaga aaagatctgc cacgtaattg ctcggggaaa
     5641 ataattagaa agaatctgaa ataaatgaaa cgtcagggaa ctaccctgcc agctaataat
     5701 cacgccctaa aacaatatgc attttttgca gggatgttgt cgtccctaaa aaagcaaaaa
     5761 tggagaaaag gaatgagtga atcattacat ctgacccgca atggatcaat tctggaaatt
     5821 acccttgatc gcccaaaagc gaatgctatt gatgcaaaaa ccagctttga aatgggcgaa
     5881 gtatttctaa atttccgtga cgatccgcaa ttacgtgtcg ccattattac cggtgccgga
     5941 gagaaattct tttccgcggg ctgggattta aaagcggcag cagaaggcga agcaccggat
     6001 gctgactttg gcccgggtgg ttttgcggga ttaaccgaaa ttttcaatct cgacaaacca
     6061 gttatcgcag ctgtgaacgg ctacgccttt ggcggcggtt tcgaactggc gctggcggca
     6121 gattttattg tttgtgccga taacgccagc ttcgccctgc cggaagccaa actgggcatc
     6181 gttcctgaca gcggcggtgt gctgcgtctg ccgaagatcc tgccgcctac catcgtcaat
     6241 gaaatggtga tgaccggcag acgaatgggc gcagaagagg cgctgcgttg ggggatagtc
     6301 aaccgcgtgg ttagccaggc ggaactgatg gataacgccc gcgaactggc tcagcagctg
     6361 gttaacagcg ccccgctggc gattgcggcg ctgaaagaga tctaccgcac caccagcgaa
     6421 atgccggtag aagaagcgta tcgctatatt cgcagcggcg tgttgaaaca ctatccatcg
     6481 gttctgcatt cggaagatgc cattgaaggg ccgctggcgt ttgccgagaa gcgcgatccg
     6541 gtgtggaaag gacgttaaca accatgagct attacgcctt tgaggggtta attccggtgg
     6601 ttcacccgac ggcgtttgtc catcccagtg ccgtcttgat tggcgatgtg attgtgggag
     6661 ccggtgtcta catcggccca ctcgcctcac tgcgtggtga ctacgggcgg ttgatcgtgc
     6721 aagcgggagc caatattcag gatggctgca ttatgcatgg ctactgcgac actgacacca
     6781 tcgttgggga aaacggccat atcgggcatg gagcgatcct gcatggttgt gtgattggtc
     6841 gcgatgcatt ggtcgggatg aacagcgtaa ttatggatgg cgcggtcatt ggcgaagaga
     6901 gcattgttgc cgcaatgagc tttgtcaaag caggctttag tggcgagaaa cgccagttgt
     6961 tgatgggtac gccagcccgc gctgtacgca gtgttagtga cgacgagtta cactggaaac
     7021 ggttgaatac caaagagtat caggatcttg tcgggcgctg tcatgcatcg ttacatgaaa
     7081 cgcagccact gaggcaaatg gaggaaaatc gcccccgttt gcaggggacg acggatgtga
     7141 cgcctaaacg gtgatcttgc cggatgcgac ctggttgctg gataagatgc tacaacatcg
     7201 tatctggcaa gcaggacgcc cggtatcaac gagataaaat taacgacaca tactctttga
     7261 caacatcatc tgccacttct gctcgcggtt aagttccgtg agcggaacgg caggaatacg
     7321 cggtgtcttt cttacgccga ctaatttttc ccgactgtta ttgcgcaggc gcgcgtaaat
     7381 ttgctcatcg atatcgacca ctttaaccag tcgctgacac tggcatcccc gcccttccag
     7441 cttattaggg atcatcttaa cttcgcagct tatttctgtg acttccgaca gaatataagt
     7501 cagggtatta accgccttgc tgtgttcaat atcgaaatga atagagatct ctcttgctat
     7561 cacccaccga ttttcagcca tcatccattc ggcgattaac aagtagagtg gtttttcaac
     7621 atatccttca cacatacgaa ctccagtttc acaaattaaa agtctagcta tgactcgttt
     7681 tgaagcaatt aaacaaggcc atattaaaat tgtggatata tcgatagtat gcaacttcac
     7741 cgtcgataag tgtgagctaa atcctgctta tgtcatcaaa aatatcgatt ccccaaaaga
     7801 tttacttaat gggcaaaaaa aaacggtcct catcagagaa ccgtattagt tattcgatca
     7861 aattagcgga tgaaaaatat ccgccacgac acgctattat ttgatctgtg cgtgcatttc
     7921 ctgcaccgaa attacttttt cagtcgcatc ggcattcagc gccatcgcgg tagcgaaacc
     7981 accgttcagg gtggtgtcat aatgcacttt atattgcagc gcactgcgac ggatcacgcg
     8041 ggagtcttca atcgcacgac ggcctgaggt ggtgttgatg atgtaggtat attcgccatt
     8101 cttgatacgg tcctgaatgt gcggacggcc ttcatgcacc ttgtttacca gacgcggatt
     8161 gatacccgct tcgcccagca caatcgccgt gccgtgggtc gcatccagct cgaagccctg
     8221 tttcagcagt tttgccgcca ggtccaccac gcgttcttta tcgccttcgc gcacggaaag
     8281 cagcgcacga ccgtgtttct tcatggtgga gttgctgccc agctgcgctt tggcgaacgc
     8341 ttcagcgaag gtgcggccca cgcccatgac ttccccggta gagcgcattt ctggccctaa
     8401 cagcgggtca acgcctggga atttattgaa cggcagcacc acttctttca ccgagtagta
     8461 cggcggaata acttctttgg ttacgccctg ctcagccagc gatttgccag ccatcacacg
     8521 cgccgccact tttgccagcg gtacgccggt ggctttggag acgaacggaa cagtacgcgc
     8581 cgcacgcggg tttacttcaa tcaggtaaac ttcgttgttt ttcaccgcaa actgcacgtt
     8641 catcagaccg cgcacctgca attcgaaggc cagtttctgc acctgctggc gcatcacatc
     8701 ctgaatttcc tgacttaagg tgtacgctgg cagcgaacat gcggagtcac cggagtgcac
     8761 acccgcctgc tcgatgtgct ccatgatgcc gccaatcagc accatttcgc cgtcgcagat
     8821 ggcatccacg tcaacttcta ctgcgtcatc aaggaaatga tccagcaaca ctggcgcatc
     8881 gttagacacg ctaaccgccg tctggaagta gcgacgcagg tcagcttcgt catagacgat
     8941 ttccatcgcc cgaccgccga gaacgtaaga cggacgcacc accagcgggt agccaatctc
     9001 ttttgccttc tcaactgcca tttcaatagc agtaacggtg gcgttcgccg gttgtttcag
     9061 tttcagacgg tcaaccgcat gctggaagcg ttcacggtct tccgcacggt cgatagcatc
     9121 cgggctggtg ccgataaccg gtacgccagc ggcttccagt gcacgcgcca gtttcagcgg
     9181 ggtctgaccg ccgtactgaa cgataacgcc tttcggcttc tcaatacgca cgatttccag
     9241 cacatcttcc agagttaccg gctcgaagta gaggcgatcg gaagtgtcgt agtcggtaga
     9301 gacggtttcc gggttacagt taaccataat ggtttcgtaa ccgtcttcac gcagcgccag
     9361 cgaggcgtgt acgcagcagt agtcgaattc gataccctga ccgatacggt ttggaccgcc
     9421 gccaagcacc atgatttttt cacggtcggt agacggattc gcttcgcact cttcttcata
     9481 agtggagtac atgtaagcgg tgtcggtggc aaactctgcc gcacaggtat ccacgcgctt
     9541 gtagaccggg tgcaggtcat attggtcacg cagcttacgg atttccgctt cgcgtacgcc
     9601 cgccagtttt gccaagcgcg catcggcaaa gcctttgcgt ttcagctggc gcaggaattc
     9661 agcgttcagg ccagtgatgc ccacttccgc cactttctct tccagacgca ccagttcttc
     9721 aatctgtacc aggaaccagc ggtcaatgtt agtcaggttg aagacgccgt ccacagacag
     9781 gcccgcgcgg aaagcatcgg cgatgtacca gatacgctct gcgcctgcgt ctttcagttc
     9841 gcgacggatt ttggttaacg cttccgggtc atccaggctc actttcgggt cgaagccagt
     9901 tgcgcccact tccaggccgc gcagcgcttt ttgcagggat tcctgctgcg tgcgaccaat
     9961 cgccatcact tcgccaaccg atttcatctg agtggtcaga cggtcgttag caccggcgaa
    10021 tttttcgaag ttgaagcgag gaattttagt aaccacatag tcgatggacg gctcgaagga
    10081 ggccggagta cgtccgccag tgatgtcgtt catcagttcg tctagggtgt aacccaccgc
    10141 cagtttcgcc gccactttag caatcgggaa accggtcgct ttcgacgcca gcgccgaaga
    10201 acgggacacg cgtgggttca tttcgataac aatcaggcga ccgtttttcg ggttcaccgc
    10261 aaactggaca ttggaaccac cggtttcaac gccgatttca cgcagcaccg ccatcgaggc
    10321 gttacgcatg atttgatatt ctttgtcggt cagcgtttgg gctggcgcga cagtgatgga
    10381 gtcaccggtg tggatgccca tcgcatcaaa gttttcgata gagcagacga tgatgcagtt
    10441 gtcgttttta tcacgcacca cttccatctc gtactctttc cagccgatca gcgactcatc
    10501 aatcagcaac tctttggtcg gagagagatc cagaccgcgg gcgcaaattt cttcaaactc
    10561 ttcacggtta taagcgatac cgccgccgct accgcccatg gtaaaggatg ggcgaataat
    10621 gcacgggaag cccacgtcag cggcaacagc cagcgcttct tccatcgtgt gtgcgatacc
    10681 ggaacgcgcg gtttccagac caattttctt catcgctacg tcgaaacgac ggcggtcttc
    10741 tgctttatca atcgcatcgg cagtggcacc aatcatggtg acaccgaact cttccaacac
    10801 gccctgacgt tccagctcca gcgcgcagtt cagcgccgtc tgaccgccca tggttggcag
    10861 caccgcgtcc gggcgctctt tttcaataat cttgcgtacc acttcccagt gaatcggctc
    10921 gatgtaggtc gcatcggcca tttccgggtc ggtcatgatg gtcgccgggt tggagttcac
    10981 cagaatgacg cggtaaccct cttcgcgcag ggctttacat gcttgcgcgc cagagtagtc
    11041 aaactcacac gcctgaccga taacaatcgg gcccgcaccc agaatcagga tactttttat
    11101 atctgtacgt tttggcatgg ctcttttact cctgattact tagcggtttt acggtactgc
    11161 tcaattaact tgataaagtg gtcgaacaac ggcgcggcgt cgtgtggacc agggctggct
    11221 tcagggtgac cctggaagct gaacgccggc ttatcgatgc gatgaatgcc ctgtaacgta
    11281 ccgtcgaaca gggatttatg cgtgacacgc aggtttgcag gtaatgttgc ttcatccacc
    11341 gcaaaaccgt ggttctgggc ggtgatcatc accacgtttt tctccacatc tttaaccgga
    11401 tggttgccgc cgtggtgacc aaatttcatt ttgacagtct tcgcaccgct cgccagcgcc
    11461 agcagctgat gaccgagaca gatgccgaat accggaatat cggtttcgag gaacttctgg
    11521 atggcggtaa tggcgtaatc gcacggggcc gggtcgccag gaccgttgga gaggaagatg
    11581 ccgtctggat tcattttcag cacatcttcc gcagaagttt gcgccggaac gatggtcagg
    11641 cgacagcctc tatccaccag catccgcagg atgttgcgct tggcaccaaa atcataagcc
    11701 acgacgtgga acggcagctc gtcttctttt ttcgcttctg gcaggccgcc ggtcaacgtc
    11761 cagctccctt gtgtccagct ataggcttct gcggtggtca cttcttttgc cagatccatg
    11821 ccgttcagac ctgggaacgc gcgggctttt tctaacgcca gcgccgcatc cgggttatcg
    11881 cctgcgataa tgcagccatt ctgtgcgcct ttctcgcgca gtaaacgcgt cagcttacgg
    11941 gtatcgatat cggcaatcgc cacgatgtta tggcgcttca ggtaagaaga gaggtcttcg
    12001 gtattacgga agttgctggc aatcagcggc aggtcgcgaa tcaccagacc ttgtgcatgt
    12061 acctgagaag attcttcatc ggcgtcatta gtgccgacat tgccaatatg gggataagta
    12121 agagtaacga tttgacgaga ataggaagga tcagtgagga tttcttgata accggtcatt
    12181 gaagtattga aaacgacttc cccaaccgcc gaacctgttg cccctatggc ccgaccgtga
    12241 aactgggttc cgtcttccag aaccaatagc gctgacttaa tcaaaacacc ctccagagaa
    12301 tactcactca ctttatttgc atattaattc acagtgatga cgtaaatcaa tgcaaatctg
    12361 cttaccgacg aatttctggc aaacggcggc attctggaga tatgggcgct aaaagtcaac
    12421 ttaatggcag gattttttat attattttgt ggctcatgca tgttttacag gcattaagcg
    12481 ac
//
LOCUS       DAZSNO010000105        12284 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZSNO010000105 DAZSNO010000000
VERSION     DAZSNO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12284
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.105"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            758..841
                     /gene="dinQ"
                     /locus_tag="ACHWN2_002841"
     CDS             758..841
                     /gene="dinQ"
                     /locus_tag="ACHWN2_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR8932297.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHWN2_002842"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHWN2_002842"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR8932298.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHWN2_002843"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHWN2_002843"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR8932299.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="ACHWN2_002844"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="ACHWN2_002844"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR8932300.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHWN2_002845"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHWN2_002845"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR8932301.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="ACHWN2_002846"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="ACHWN2_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            7850..7903
                     /locus_tag="ACHWN2_002847"
     CDS             7850..7903
                     /locus_tag="ACHWN2_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932302.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="ACHWN2_002848"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="ACHWN2_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR8932303.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="ACHWN2_002849"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="ACHWN2_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR8932304.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="ACHWN2_002850"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="ACHWN2_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR8932305.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="ACHWN2_002851"
     CDS             10967..12166
                     /locus_tag="ACHWN2_002851"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR8932306.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3002 a         3125 c         3298 g         2859 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggcgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgcc
//
LOCUS       DAZSNO010000106        12283 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZSNO010000106 DAZSNO010000000
VERSION     DAZSNO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12283
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.106"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACHWN2_002852"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACHWN2_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR8932307.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="ACHWN2_002853"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="ACHWN2_002853"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR8932308.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="ACHWN2_002854"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="ACHWN2_002854"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR8932309.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="ACHWN2_002855"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="ACHWN2_002855"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR8932310.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="ACHWN2_002856"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="ACHWN2_002856"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR8932311.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="ACHWN2_002857"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="ACHWN2_002857"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR8932312.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="ACHWN2_002858"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="ACHWN2_002858"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR8932313.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="ACHWN2_002859"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="ACHWN2_002859"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR8932314.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /gene="hisIE"
                     /locus_tag="ACHWN2_002860"
     CDS             7139..7750
                     /gene="hisIE"
                     /locus_tag="ACHWN2_002860"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR8932315.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACHWN2_002861"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACHWN2_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR8932316.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACHWN2_002862"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACHWN2_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACHWN2_002863"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACHWN2_002863"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR8932317.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..>12283)
                     /locus_tag="ACHWN2_002864"
     CDS             complement(11944..>12283)
                     /locus_tag="ACHWN2_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932318.1"
                     /translation="ESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKGMAIGDVF
                     GLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYPKFKVLMD
                     TPNGILIAIK"
BASE COUNT         3092 a         3121 c         3074 g         2996 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tct
//
LOCUS       DAZSNO010000107        12277 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZSNO010000107 DAZSNO010000000
VERSION     DAZSNO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12277
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.107"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..98
                     /locus_tag="ACHWN2_002865"
     CDS             <1..98
                     /locus_tag="ACHWN2_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8932319.1"
                     /translation="FEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            95..496
                     /locus_tag="ACHWN2_002866"
     CDS             95..496
                     /locus_tag="ACHWN2_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR8932320.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            507..1247
                     /locus_tag="ACHWN2_002867"
     CDS             507..1247
                     /locus_tag="ACHWN2_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR8932321.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1306..1692
                     /locus_tag="ACHWN2_002868"
     CDS             1306..1692
                     /locus_tag="ACHWN2_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR8932322.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1701..2030
                     /locus_tag="ACHWN2_002869"
     CDS             1701..2030
                     /locus_tag="ACHWN2_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR8932323.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2002..5043
                     /locus_tag="ACHWN2_002870"
     CDS             2002..5043
                     /locus_tag="ACHWN2_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR8932324.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5043..5372
                     /locus_tag="ACHWN2_002871"
     CDS             5043..5372
                     /locus_tag="ACHWN2_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8932325.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5372..6070
                     /locus_tag="ACHWN2_002872"
     CDS             5372..6070
                     /locus_tag="ACHWN2_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR8932326.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6075..6818
                     /locus_tag="ACHWN2_002873"
     CDS             6075..6818
                     /locus_tag="ACHWN2_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR8932327.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6782..7357
                     /locus_tag="ACHWN2_002874"
     CDS             6782..7357
                     /locus_tag="ACHWN2_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR8932328.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7418..10897
                     /locus_tag="ACHWN2_002875"
     CDS             7418..10897
                     /locus_tag="ACHWN2_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8932329.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10965..11564
                     /locus_tag="ACHWN2_002876"
     CDS             10965..11564
                     /locus_tag="ACHWN2_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR8932330.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11628..>12277
                     /locus_tag="ACHWN2_002877"
     CDS             11628..>12277
                     /locus_tag="ACHWN2_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR8932331.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAA"
BASE COUNT         2892 a         2917 c         3930 g         2538 t
ORIGIN      
        1 catttgaaga tgcccgcgac cgcatcattg ctgcggaaat gccgaaacag ctggggtatg
       61 ccctgaaaca acaactgagg ttacatctga gtaaatgaac cgacacacac aaatccgtca
      121 gagcgtgctg gcacgccttc gggaacagtg tggagacagc gccacgtttt ttgacgggct
      181 tccggcattt attgatgcgc aggaactgcc cgccgtggcg gtgtggctga gtgatgcgca
      241 gtacaccgga aaaatgacgg atgaggacga ctggcaggct gttctgcata ttgccgtttt
      301 cgtccgtgca caggcgccgg attctgagct ggatacgtgg atggagaaca ccatatttcc
      361 ggccatgaag gatattccgg tgctttccgg actcatcgac accatgatcc cactcggttt
      421 taactatcaa cgtgataatg agatggccac ctgggcgatg gcggaaatca cgtaccagat
      481 cacgtacacg aattaaggag gtggtaatga ccacaccaaa tccactggca aagacgaaag
      541 gtgcggggac gacgttctgg atgtataccg gcaacggcga tgcgtttgcg aaccctttgt
      601 cggacactga ctggctgcgt cttgcgatgg tgaaggatct gcaacctggc gaaatgaccg
      661 ctgatgcaga agatgacact tatctcgatg atgaagatgc agactggaaa acgacaaccc
      721 aggggcagaa atccgttggt gatacttcgg cgacgctggc ctggcgtccg ggtgacagcg
      781 ggcagaaaaa actggttcag ttgttcgact ccggtgaagt ctgcgcgttt cgtatcaaat
      841 atcccaacgg cactgttgat gttttccgcg gctggctgag ctcactgggt aaaaccattg
      901 cctcaaaaga cgtgatgacc cgcactgtga aaatcagcgg tgtggggcgt ccgtatctgg
      961 cagaggaagg cactgaaacc gtgggtgtta ccggactgac ggtggcaccg gcatctgtca
     1021 gtgtcaaagc gggagcaacc accacactga cctttacagt aaaacctgac ggggccagtg
     1081 acaaagcgat cagtgtgcat tcgtcagatc ctcagactgc ttcggtgacc ctgagcgggc
     1141 ttgtggccac ggtgaaaggc gtgaagcagg gcagtgtcag catcgtgggc atgacctctg
     1201 acggagagtt tgtggcagtg gctgcggtga ccgtcagcgc accataacag gacgatactc
     1261 atcattgccc cggttatccg gggctttttg catccggaga acatgatgtt tctgaaacag
     1321 gatacgttta attatgaaaa acagtccgtg gtgctcagtg agctgtccgg gctgcagaga
     1381 attgaatatc tgacgtttgt tcagcagcga acggcaaagt ttgatgcaca ggagggagaa
     1441 ctgccggagg ctgaacgaca gattgctttt ctgcgtatgg gaatggatat caatgcctgg
     1501 ctggtttccc gctcactgtg gaatgctgag cagtctcagg atgttgagac gctttgcgca
     1561 tccattatga caacatggtc gtatgatgcg ctgggcgcgg gggcggagag ggttctgtcg
     1621 ctgagcggta tggggaccat tgagaatgcc ggggatgatg atcatgaggc gctgacgccg
     1681 gaaaagtcct gacgcgggaa atgcagtttg tcatgcggct tgcccgggag ttccggcggg
     1741 cagactggcg gcggatgctg tcggatatgt cggccactga gcttggtgag tggggcgatt
     1801 atttccggat gcagagcttc agtgatgtgt ggatggatgc gcagtttgcc tcgctgaagg
     1861 cattgatcgt gagaatggtg tccggcagca gcgatgctgc ggtggctgat ttcagccttt
     1921 taccggaaga gaacgggata ccggagcgaa cggacgaaga actgatgcat cttggggaag
     1981 gtatttccgg aggtgtgcgt tatggaccag atagccaacc tggtcattga tttggggatt
     2041 gatgcggcag agtttaaaaa tgaaattccc cgtatcaaaa accttctgaa tggtgcagcc
     2101 agcgatgcag aacggtcttc tgcccgtatg cagcgtttta tggagcgtca gactcaggcg
     2161 gcccggcaga caatgcaggc ggcttcttcg gctgcaacag cagcatcagc ccatgcgcag
     2221 acggtggaga agaatgcacg ggctcatgaa cgcatggccc gtgaggtgga acaaacccgt
     2281 ctgcgcgtgg atgccctgaa tcagaaaatg cgcgaggaac aggcgcaggc cagggcactg
     2341 gcggaggcgc aggataaagc ggctgccgcc ttttatcgcc agattgacag tgtgaaacag
     2401 gccggtgcgg ggcttcagga attacagcgt attcagcagc agatccgaca ggccagaaac
     2461 agtggcgggg ttggtcagca ggattatctg gcgctgattt cggagatcac ggcgaaaacc
     2521 cgtgccctga cgcaggcaga ggaacaggcc acccggcaga aagcggcgtt tatccgccag
     2581 cttaaagagc aggcaacccg ccagaatctg tcgtcttctg agttgcttcg cgccagggcg
     2641 gctcagctgg gggtaagcag tgctgcagaa gtgtatatcc gcaaaatgga gcgggcagga
     2701 aaagccacac attcgctggg gctgaaaagt gcggcagccc ggcgggagct gggggtgtta
     2761 atcagtcaga tggcgcgagg caattttggt gcgctgaggg gatccgggat aacgctggct
     2821 aaccgtgccg gatggacagg cgcactgatg tcgccgaaag gcatgatgac tggcggcgtt
     2881 attggcggac ttgtcgcggc ggtcctgggt ctgggtaaag cctggcatga cggccggaag
     2941 gagggcgaga aatttaaccg tcagctggcg ctgacgggac attatgccgg tgtcactgtc
     3001 gggcagttgt ggaaactcag ccgggccata tccgggaatg gtatcacgca acatgcggca
     3061 gccggtgcgc tggcacaggt ggtgggcagt ggggcatttc atggaaacga tattggtatg
     3121 gtggcgaaag ctgccgcaca gatggagcga tcggttggcc agtcggtcag cgataccata
     3181 aatcagttta agcggctgaa ggatgatcct gtaaatgccg cgaaggcgct ggacaatgcg
     3241 ctgcattttt tgaatgccac tcagcttgag cagatacgcg tccttgggga gcaggggcgg
     3301 tccagtgatg cggcccggat cgccatgtct gcgctggcag aggaaaccgg taaacgcaca
     3361 tctgatattg ataataatct caatgcgctg ggtagtacgc tgcaaacctt gtctgactgg
     3421 tggaagcagt tctgggatgc ggccatgaat attggtcgtg aagactcgct ggatgcgcag
     3481 attgatgcgt tacaggaaaa aattcagcgc gcgaaaaaat atccgtggac aaacgcctcc
     3541 acacaggtgg agtacgatca gcagcgtctt aacgatcttc aggagaaaaa acgccggaag
     3601 gatttgcagg atgcaaaagc gcaggcagaa cggaattacc aggagcaaca gaaacgccgg
     3661 aatgctgaaa atgccgcgct gaaccggatg aatgaaacgg aagctgcacg acatcagcgg
     3721 gaaattgcgc gtattaatgc catgcagtac gccgaccagg ctgtcaggga tgcggcgata
     3781 cagcgtgaaa atgaacgtta cgaaaaagcc attaagaaaa atacacgggc aacccgtaat
     3841 gatgaggcca cccggttatt gctgcagtac agtcagcagc aggcacaggt ggaagggcag
     3901 attgctgctg ccagacagtc agcaggcatt gccactgaaa ggatgactga agcgcataaa
     3961 cagcttctgg ccctgcagca gcgcatcagc gacctggacg ggaaaaagct gacggcagat
     4021 gaaaagagtg tgctggcccg taagaatgag ctgattcagg cgctgacgct gctggatgtg
     4081 aaacagcagg agctgcagaa acagacagcg cttaacgacc tgagaaaaaa aacggttcag
     4141 ctgaccagcc agctggcaga caaagaacgt gcactgcgtg agcagcacaa tctggatatt
     4201 gccactgcag gtatggggga taagcagcgg cagcgctacc aggcacagtt gcgcatccgg
     4261 caggaatacc ggcaacagtt gcaacagctt gagaatgaca gtcgccagaa aggcacttac
     4321 gggacggagg actaccggag ggctgaggag gtgctgaagg ggagcctgaa gcgacaactg
     4381 aatgaaaaca aacgctactg gcaggaactg gaagtggcgc agggcgactg gaaaaacggt
     4441 gccatgcggg cgtttcagaa ttttacggcg gatgcggata atgcggcggg aacggcagaa
     4501 cagatgttta cagtggcatt cagcagtgcc ggtaatgcac tggcgacatt ctgtaccacc
     4561 ggtaagctga attttaaatc cttcacctct tccctgttgt cagatatggc cagaattatg
     4621 gcacagatgg ccatgatgca ggcggtaaag ggcgtcggtt ctttattcgg cttcacgact
     4681 aatgctgatg gcggtgttta ccagtctgct gatttgagtc gctacagtgg cacggtggtt
     4741 aaccgtccga cgttttttgc ttttgcaaaa ggcgcgggtg tgatggggga agcgggacct
     4801 gaagccattc tgccactgcg tcgcggtgct gatggtaagc tgggggttgt ggcggatact
     4861 ggtggttcag gcatggtgat gtttgccccg cagtacaaca ttgagatcaa taacgacggc
     4921 acgaacgggc agataggtcc ggctgccctg aaggtggttt atgacctcgg gaaaaaagcg
     4981 gccgcggact ttatgcaaca gcaggcccgt gatggtggcc ggttaagtgg agcataccgg
     5041 taatggagac gtttcactgg aaagtgcgcc cggatatgaa tgtggtatca gagccgaaag
     5101 tggtgacagt gaagctgggc gatggttatg aacagcgtcg tgcggcggga ctgaataacc
     5161 agttgtcgac ttacagcgtg acgatacgtg ttcgtaaagg tgaacaccca tctttaaaag
     5221 cctttctgga acggcacggt ggcgtccgcg catttcagtg gacgccacct tatgactgga
     5281 aactgatcag ggtggtttgt cgtaaatggt cggcaagcgt gggggcgtta tgggtgactg
     5341 taacggccga ttttgaacag gtggtgaact gatgcaggat atccggcagg aaacactgaa
     5401 tgaatgcacc cgtgcggagc agtcagcccg ggtggagctc tgggaaatcg atctgacaga
     5461 ggtcggtggt gagcgttatt ttttctgtaa tgagcagaac gaaaaaggtg agccggtcac
     5521 ctggcagggg cggcagtatc aggcataccc cattcagggg tcggggtttg aactgaacgg
     5581 caggggctgt gctgcccgtc cgacactgac ggtctctaac ctgcacggca tggtcaccgg
     5641 gatggcggaa gatctgcaga gtctggtcgg cggaacggtg gtccggcgta aggtttacgc
     5701 ccgttttctg gatgcggtga acttcgtcaa cggaaacagc gacgccgatc cggaacagga
     5761 ggtgatcagc cgctggcgca tcgagcagtg cagcgaactg agcgcggtca gtgcctcttt
     5821 tgtgctggcc acaccaacgg agacggatgg cgcggttttc ccggggcgta tcatgctggc
     5881 gaatacctgt atgtggactt accgttctga tgagtgtggt tacacgggca gggcagtggc
     5941 tgacgagttc gacaaaccaa caacggatat ccggaaggac aaatgcagca agtgtatgcg
     6001 cgggtgtgag ttgcgcaaca ataccggtaa tttcggcggt ttcctttcca tcaataaact
     6061 ttctcagtaa atccatgaca cagacagaat cagcgattct ggcgcacgcc cggcgatgtg
     6121 cgccagcgga gtcgtgtggc ttcgtggtga gaacgccgga gggagacagg tatcttccca
     6181 gcgagaatat ctccggtgag ccggaggaac ggttccggat ggctccggag gactggctgc
     6241 gggcacaaat gcagggtgag attgtggcac tggtccacag tcatcccggt ggtctgccct
     6301 ggctgagtga ggctgaccgg cggctgcagg tgcagagtga tttgccgtgg tggctggtct
     6361 gccgggggac gattcacaag ttccgctgtg tgccgcatct caccgggcgg cgctttgagc
     6421 acggggtgac ggactgttac acgctgttcc gggatgctta tcatctggcg gggattgaga
     6481 tgccggattt tcatcgcggg gatgactggt ggcgtcacgg tcagaatctc tatctggata
     6541 atctggaggc cacagggctg tatcaggtgc cgttgtcatc agcacaaccg ggcgatgtgc
     6601 tgctgtgctg ttttggttca tcggtgccga atcatgccgc catttactgc ggcgatggtg
     6661 agctgctgca ccatattcct gaacaactga gtaaacgaga gaggtatacc gacaaatggc
     6721 agcgacgcac acactccctc tggcgtcacc gggaatggca cgcatctgcc tttacgggga
     6781 tttgcaacga tttggccgcc gcatcgacct tcgtgtgaaa acgggggctg aagccatccg
     6841 cgcactggcc acacagctcc cggcgtttcg tcagaaactg agtgacggct ggtatcaggt
     6901 gcgcattgcc gggcgtgatg caggtgaaac cgaattgtct gcccgtctta atgagccgct
     6961 ggcaaatggt gcagtgatcc acatagtacc gcgtctggtg ggagctaaaa gtggcggtgt
     7021 gtttcaggcg gtgctggggg cagctgttat ggcggttgct atatggatgc cgggggtagg
     7081 aattatggcg agtaatctgc tgttttctct cggtgccagt atgacgcttg gcggtgttgc
     7141 acagatgctg gcaccgaaag ccagaactcc ccgtacacag acaacggata acggcaaaca
     7201 gaacacctgt ttctcctcac tggataacat ggttgcccag ggcaatgttc tgcctgttct
     7261 gtacggtgaa atgcgcgtgg ggtcgcgggt ggtatctcag gagatcagca cggcagacga
     7321 aggggatggt ggtcaggttg tggtgattgg tcgctgatgc aaaatgtttc atgtgaaacc
     7381 gcctgcgggc ggttttgtcg tttatggagc gtgaggaatg ggtaaaggca gcagtaaggg
     7441 gcataccccg cgcgaagcga aggacaacct gaaatccacg cagttgctga gtgtgattga
     7501 tgccatcagc gaagggccgg ttgacggtcc ggtggatgga ttaaaaagcg tgctgctgaa
     7561 cggtacgccg gtcctggata gcgaggggaa gacaaacttt tccggtgtta cggtggtgtt
     7621 ccgcgccggc gagcaggagc agacaccgcc ggaggggttt gaatcttccg gctcagagac
     7681 tgtgctgggt acggaagtga aatacgacac gccgatcacc cggaccatca cgtcggcaaa
     7741 cattgatcgt ctgcgcctga ccttcggtgt gcaggcactg gtggaaacca cctcaaaggg
     7801 ggaccggaat ccgtcggaag tccgcctgct ggttcagata cagcgtaacg gtggctgggt
     7861 gacggaaaaa gacatcacca ttaagggcaa aaccacttcg cagtatctgg cctcggtggt
     7921 ggtgggtaac ctgccgccgc gcccgtttaa tatccggatg cgcaggatga caccggacag
     7981 caccacagac cagctgcaga acaaaacgct ctggtcgtcg tacaccgaaa tcatcgatgt
     8041 gaaacagtgc tacccgaaca cggcgctggt cggcgtgcag gtggattcag agcagttcgg
     8101 taaccagcag gtgagtcgca attatcatct tcgcgggcgc attctgcagg tgccgtcgaa
     8161 ctataacccg cagacgcggc aatacagcgg tatctgggac ggaacgctta agccagcata
     8221 cagcaacaac atggcctggt gtctgtggga catgctcact catccgcgct acggcatggg
     8281 gaaacgtctt ggtgcggcag atgtggacaa atgggcgctg tatgtcatcg gccagtactg
     8341 cgaccagtca gtgccggacg gatttggcgg cacggagccg cgcatcacct gtaacgctta
     8401 cctgaccaca cagcgtaagg cgtgggatgt tctcagtgat ttctgctcgg cgatgcgctg
     8461 tatgccggta tggaacgggc agacgctgac gttcgtgcag gaccgaccgt cggataaagt
     8521 gtggacctat aaccgcagta atgtggtgat gccggatgat ggcgcgccgt tccgctacag
     8581 cttcagcgcc ctgaaggacc gccataatgt cgttgaggtg aactggattg atccggacaa
     8641 cggtcatgag acggcgacag agcttgtgga ggacacgcag gccattgtcc gttacggtcg
     8701 taacgtcacg aagatggatg cctttggctg taccagccgg ggacaggcgc accgcgccgg
     8761 gctgtggctg attaaaacgg aactgctgga aacgcagacc gtggacttca gcgtcggcgc
     8821 agaagggctt cgccatgtac cgggtgatgt cattgaaatc tgcgatgatg attatgccgg
     8881 tatcagcacc ggtggtcgcg tactggcggt gaacagccag acccggacgc tgacgctcga
     8941 ccgtgaaatc acgctgccgt cctccggtac cacgctgata agcctggttg acggaaatgg
     9001 caatccggtc agcgtggagg ttcagtccgt caccgacggc gtgaaggtga aagtgagccg
     9061 ggttcctgac ggcgttgcag aatacagcgt gtgggggctg aagctgccga cgctgcgcca
     9121 gcgcctgttc cgctgcgtga gtatccgtga gaacgacgac ggcacgtatg ccatcaccgc
     9181 cgtgcagcat gtgccggaaa aagaggccat cgtggataac ggggcacact ttgacggtga
     9241 ccggcgcggc acggtgaatg gtgtcacgcc gccagcggtg cagcatctga ccgccgaagt
     9301 caccgcagac agcggggaat atcaggtgct ggcgcgatgg gacacgccga aggtggtgaa
     9361 gggtgtgagc ttcctgcttc gcctgaccgt ggcagcggac gacggcagtg agcggctggt
     9421 cagcacagcc aggacgacgg aaaccacata ccgcttcagg cagctggcgc tggggaatta
     9481 cagtctgaca gtccgggcgg taaatgcccg ggggcagcag ggcgatccgg cgtcggtatc
     9541 gttccggatt gcggcaccgg cagcgcctgt cactattgaa ctgataccgg ggtattttca
     9601 gataacggtg gtcccgaaac tggctgtata tgacccgacg gtgcagtttg agttctggtt
     9661 ttcggaaaag cggattgcgg atatcaggca ggttgaaacc agcgcccgct atcttggcac
     9721 ggcgctgtac tggatagccg ccagtatcaa tatcaggccg ggccatgatt attattttta
     9781 cgttcgcagt gtgaacaccg ttggcaaatc ggcatttgtg gaggccgtcg gtcgggcgag
     9841 cgatgatgcg gaaggttatc tgagttttta taaagggttg atcaataaaa cgcatctcgg
     9901 caaggagttg tggacgcaga ttgataacgg tcagcttgcg ccggacctga ctgaaatcag
     9961 gacgtccatt acgaatgtca gcaatgaaat cacgcaaacc gtcaataaaa aactggaaaa
    10021 tcagagtgct gcaatccagc agatacagaa agttcaggtt gatacaaata ataacctgaa
    10081 cagcatgtgg gctgtgaagc tgcaacagat gaaggacgga cgcctttata ttgcgggtat
    10141 cggtgccggt attgagaata cgccagcagg aatgcagagt caggtgctgc tggcggcaga
    10201 caggattgcg atgattaatc ctgcgaatgg caacacaaag ccgatgtttg ttggtcaggg
    10261 cgatcagata ttcatgaacg acgtgttcct gaaacgcctg acggctccga ccattaccag
    10321 cggcggtaat cctccggcat tttccctgac acctggcgga cggctgacgg cgaaaaatgc
    10381 cgatatcagc ggtaacgtga acgcgaactc cgggacgctc aacaacgtca cgattaataa
    10441 gaactgtcgg gttctgggaa aattgtccgc gaaccagatt gaaggcgatc tcgttaaaac
    10501 agtgggcaaa cctttcccac gggactcccg ggcaccggag aggtggccat cagggaccat
    10561 taccgtcagg gtttatgacg atcagccgtt tgatcggcaa attgttattc ccgcggtggc
    10621 gtttcgcggt gctaaacatg agcggaagaa taacaatatt tattcgtcat gccgcctgat
    10681 agtgaagaaa aacggtgctg aaatttataa ccgaacgacc ctggataata cgctgatata
    10741 tacgggtgtt attgatatgc ctgccggtca cggtcacatg acgctggagt tttctgtatc
    10801 ggcatggctg gtaaatggct ggtatcccac agcaagtatc agcgatttgc tggttgttgt
    10861 gatgaagaaa tccacagcag gtatcagtat cagctgaatt ttataaccca gatacgggca
    10921 ccagaaatgg tgcctttttt attgcagaaa agcgagaggt aattatgcgt aaactttatg
    10981 ctgccatttt gtccgcagcc atctgtctgg ctgtatccgg tgcgcctgca tgggcgtctg
    11041 aacatcggtc cacgctgagc gcggggtatc ttcatgcctc gacgaacgct cccggcagtg
    11101 atgatctgaa cgggattaac gtgaaatacc gttatgagtt tacggacacg ctggggctgg
    11161 tgacgtcatt cagctatgcc aacgctgaag atgagcaaaa aacgcattac agtgataccc
    11221 gctggcatga ggattccgtg cgtaaccgct ggttcagcgt gatggcgggg ctgtctgtgc
    11281 gcgtgaatga atggttcagc gcgtatgcga tggcgggcgt ggcttacagc cgtgtgtcga
    11341 ccttctccgg ggattatctc cgcgtaactg acaacaaggg gaaaacgcac gatgtgctga
    11401 ccgggagtga tgacaaccgc cacagcaaca cgtctctggc gtggggggct ggcgtgcagt
    11461 ttaacccgac cgaatccgtg gccattgacc ttgcttatga aggttccggc agtggcgact
    11521 ggcgcactga cggtttcatc gtgggtgtcg gttataagtt ctgattagcc aggtaacaca
    11581 gtgttatgac agcccgctgg ttcaggcggg ctttttgtgg ggtgaatatg gcagtaaaga
    11641 tttcaggtgt actgaaagat ggggcgggta aacctgtcgt aaattgtgcg attgaactgc
    11701 gggccagaag aaccagtccg accgttgtgg cacacgttgt tgccacttgc gtgacggaca
    11761 ataacggtgc ttatgtgatt gaggctgagc cggggtatta cgaggttgcg cttcactgta
    11821 acggctggca gccaacccgt gtcggggata ttgatgtggc accgactgat gcaccgggga
    11881 cactgaacgc gtttctgaat gcaccaaagg atggtgattt acgtccggag gtgatgaagc
    11941 gctttgagga aatggtggcg caggcgcagc agagtgccgg ggctgcagcc ggaaacgcac
    12001 agcagacggc gcaggatgtg gcggcagccg caacggcccg tgatgatgca caacgttttg
    12061 cggagaaagc ccgacaggat gcaaccgtca cagctgagga cagaaaggcc actgcggaag
    12121 atgtgacaag cacaggagca aatgcagccg cagccggaca gagcgcacag gatgccgcag
    12181 gttatgcccg cgcagcagaa caggccaaaa atgacattga tgctgcgctg accggcactc
    12241 tgaaaatggc taaccatctg tcagaaatcg cagcagc
//
LOCUS       DAZSNO010000108        12272 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZSNO010000108 DAZSNO010000000
VERSION     DAZSNO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12272
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.108"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1175)
                     /locus_tag="ACHWN2_002878"
     CDS             complement(<1..1175)
                     /locus_tag="ACHWN2_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8932332.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="ACHWN2_002879"
     tRNA            complement(1374..1458)
                     /locus_tag="ACHWN2_002879"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="ACHWN2_002880"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="ACHWN2_002880"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR8932333.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="ACHWN2_002881"
     CDS             2802..4304
                     /locus_tag="ACHWN2_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR8932334.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="ACHWN2_002882"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="ACHWN2_002882"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR8932335.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="ACHWN2_002883"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="ACHWN2_002883"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR8932336.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6668..6718)
                     /gene="ytgA"
                     /locus_tag="ACHWN2_002884"
     CDS             complement(6668..6718)
                     /gene="ytgA"
                     /locus_tag="ACHWN2_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR8932337.1"
                     /translation="MSIIILATISTTQENK"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="ACHWN2_002885"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="ACHWN2_002885"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR8932338.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="ACHWN2_002886"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="ACHWN2_002886"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR8932339.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="ACHWN2_002887"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="ACHWN2_002887"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR8932340.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..>12272)
                     /locus_tag="ACHWN2_002888"
     CDS             complement(11869..>12272)
                     /locus_tag="ACHWN2_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFR8932341.1"
                     /translation="IENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNL
                     SLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTYLLGDL
                     DDLPLINKEEQPDKGFLASISRGVMPSLPFL"
BASE COUNT         3032 a         3210 c         3290 g         2740 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctatttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gccagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcccaaa caacaccaga gaaggcccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgta
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgttcggc cagctcggta atcgtgttca gcacgctttg cgggctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatcg ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtcaggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcagt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatga cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8221 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcagtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcacaccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc cgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttattga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc ataactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat at
//
LOCUS       DAZSNO010000109        12220 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZSNO010000109 DAZSNO010000000
VERSION     DAZSNO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.109"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..493)
                     /locus_tag="ACHWN2_002889"
     CDS             complement(<1..493)
                     /locus_tag="ACHWN2_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR8932342.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="ACHWN2_002890"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="ACHWN2_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR8932343.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="ACHWN2_002891"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="ACHWN2_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR8932344.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="ACHWN2_002892"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="ACHWN2_002892"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR8932345.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="ACHWN2_002893"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="ACHWN2_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR8932346.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="ACHWN2_002894"
     CDS             3647..4156
                     /locus_tag="ACHWN2_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8932347.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="ACHWN2_002895"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="ACHWN2_002895"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR8932348.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="ACHWN2_002896"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="ACHWN2_002896"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR8932349.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="ACHWN2_002897"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="ACHWN2_002897"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR8932350.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="ACHWN2_002898"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="ACHWN2_002898"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR8932351.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="ACHWN2_002899"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="ACHWN2_002899"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR8932352.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="ACHWN2_002900"
     CDS             complement(8798..9691)
                     /locus_tag="ACHWN2_002900"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR8932353.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="ACHWN2_002901"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="ACHWN2_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="ACHWN2_002902"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="ACHWN2_002902"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFR8932354.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11581..>12220
                     /gene="allE"
                     /locus_tag="ACHWN2_002903"
     CDS             11581..>12220
                     /gene="allE"
                     /locus_tag="ACHWN2_002903"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFR8932355.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3158 c         3118 g         2978 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatacaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 ccacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catccggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgct
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgttg attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgccagcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggcgc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcaggaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12061 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12121 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12181 gaacatggtg cctatattct ttccggtcag ggggtttata
//
LOCUS       DAZSNO010000110        12154 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZSNO010000110 DAZSNO010000000
VERSION     DAZSNO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.110"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            126..995
                     /locus_tag="ACHWN2_002904"
     CDS             126..995
                     /locus_tag="ACHWN2_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR8932356.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="ACHWN2_002905"
     CDS             complement(1198..1551)
                     /locus_tag="ACHWN2_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR8932357.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHWN2_002906"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHWN2_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR8932358.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="ACHWN2_002907"
     CDS             complement(3379..3672)
                     /locus_tag="ACHWN2_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR8932359.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHWN2_002908"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHWN2_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR8932360.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /locus_tag="ACHWN2_002909"
     CDS             complement(5220..5696)
                     /locus_tag="ACHWN2_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR8932361.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="ACHWN2_002910"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="ACHWN2_002910"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR8932362.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHWN2_002911"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHWN2_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR8932363.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="ACHWN2_002912"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="ACHWN2_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR8932364.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHWN2_002913"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHWN2_002913"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR8932365.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="ACHWN2_002914"
     CDS             11052..11627
                     /locus_tag="ACHWN2_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8932366.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="ACHWN2_002915"
     tRNA            11734..11809
                     /locus_tag="ACHWN2_002915"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2931 a         3140 c         2980 g         3103 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gtgctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa caat
//
LOCUS       DAZSNO010000111        12128 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZSNO010000111 DAZSNO010000000
VERSION     DAZSNO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12128
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.111"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="ACHWN2_002916"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="ACHWN2_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR8932367.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="ACHWN2_002917"
     CDS             complement(2005..2928)
                     /locus_tag="ACHWN2_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR8932368.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="ACHWN2_002918"
     CDS             3145..4488
                     /locus_tag="ACHWN2_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR8932369.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="ACHWN2_002919"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="ACHWN2_002919"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR8932370.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="ACHWN2_002920"
     CDS             6508..6732
                     /locus_tag="ACHWN2_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR8932371.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="ACHWN2_002921"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="ACHWN2_002921"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR8932372.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACHWN2_002922"
     CDS             complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACHWN2_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR8932373.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="ACHWN2_002923"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="ACHWN2_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR8932374.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="ACHWN2_002924"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="ACHWN2_002924"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR8932375.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..10971
                     /locus_tag="ACHWN2_002925"
     CDS             10315..10971
                     /locus_tag="ACHWN2_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFR8932376.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(11070..11480)
                     /gene="tnpA"
                     /locus_tag="ACHWN2_002926"
                     /pseudo
     CDS             complement(11070..11480)
                     /gene="tnpA"
                     /locus_tag="ACHWN2_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11503..12128
                     /locus_tag="ACHWN2_002927"
                     /pseudo
     CDS             11503..12128
                     /locus_tag="ACHWN2_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT         3142 a         2837 c         3047 g         3102 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatataac aattactctg atttaaaaga aacaacctca
    10441 gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac
    10501 agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg
    10561 cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg
    10621 cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt
    10681 accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta
    10741 gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt
    10801 gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc
    10861 gaaggtaaag acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt
    10921 attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg
    10981 cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg
    11041 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg
    11101 atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg
    11161 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat
    11221 acgcctttga ggctgtttac cagactggat atcgccagtt tggggggata gttaattaac
    11281 agatggacgt gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat
    11341 acatttgaaa agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg
    11401 tatctggtga caaagactag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg
    11461 atatcggttt cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca
    11521 tttaaattcc agttaagacc aggtggtcaa caggagtgtg aaatgaggcg cttcgcaggc
    11581 gcatgtcgtt tcgttttcaa tcgtgctctg gcacgtcaga atgagaatca tgaggccggt
    11641 aataaataca tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact
    11701 gaaacgcaat ggcttaaaga ttctccctca cagccattgc aacagtcact gaaagacctt
    11761 gagcgggctt acaaaaactt cttccggaag cgggctgctt ttccccgatt caaaaagcgg
    11821 tgacagaatg atgcattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt
    11881 atttttctgc cgaaactggg ctggatgcgc taccggaata gccgtcaggt cacgggtgtt
    11941 gtgaaaaatg tcactgtcag ccagtcctgc ggtacgtggt acatcagtat tcagacagaa
    12001 agtgaagtat ccactccggc tcacccttca gcatcaatgg tcgggctgga tgctggcgtg
    12061 gctaaactcg ccacgctgtc agatggcaca gtctttgagc ctgtaaacag ttttcagaaa
    12121 aaccagaa
//
LOCUS       DAZSNO010000112        12113 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZSNO010000112 DAZSNO010000000
VERSION     DAZSNO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.112"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..370
                     /locus_tag="ACHWN2_002928"
     CDS             <1..370
                     /locus_tag="ACHWN2_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932377.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="ACHWN2_002929"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="ACHWN2_002929"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR8932378.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="ACHWN2_002930"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="ACHWN2_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR8932379.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="ACHWN2_002931"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="ACHWN2_002931"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR8932380.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="ACHWN2_002932"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="ACHWN2_002932"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR8932381.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="ACHWN2_002933"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="ACHWN2_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR8932382.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..6756)
                     /gene="tar"
                     /locus_tag="ACHWN2_002934"
     CDS             complement(5095..6756)
                     /gene="tar"
                     /locus_tag="ACHWN2_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR8932383.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="ACHWN2_002935"
     CDS             complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="ACHWN2_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR8932384.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="ACHWN2_002936"
     CDS             complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="ACHWN2_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR8932385.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9394..10320)
                     /gene="motB"
                     /locus_tag="ACHWN2_002937"
     CDS             complement(9394..10320)
                     /gene="motB"
                     /locus_tag="ACHWN2_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR8932386.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10317..11204)
                     /gene="motA"
                     /locus_tag="ACHWN2_002938"
     CDS             complement(10317..11204)
                     /gene="motA"
                     /locus_tag="ACHWN2_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR8932387.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="ACHWN2_002939"
     CDS             complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="ACHWN2_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR8932388.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11912..>12113)
                     /locus_tag="ACHWN2_002940"
     CDS             complement(11912..>12113)
                     /locus_tag="ACHWN2_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR8932389.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2783 a         3388 c         2934 g         3008 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgaa ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaagaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccaga gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgccagg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagttaacgc
     6361 tgtgtaatag tttttatatt tttcatcaaa attacggctg gtagcgacca tttcaggtaa
     6421 cggtgccatg cttttgaatt ttttataatg cgtcgcggcc tgggccaatg ttttcctggc
     6481 gctatcgagc aattcaactt tggcgttact ttgttgattg gaggaatcca tcatcatccg
     6541 caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg ttgacgtcag
     6601 ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc tatggtgaag
     6661 ggaagaaaaa aacaaactgc cggaaataag ctgtaacagt gcgaataccc ccagcaccat
     6721 taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg aaaacaagtt
     6781 gatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg ccgcgaaatt
     6841 gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa atcgggagaa
     6901 ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt tcagcagttt
     6961 ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag tgagatattc
     7021 tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg tcaatgaaag
     7081 cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat tcaggacgat
     7141 aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc gtaagtcaac
     7201 aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg gcgtgttcgc
     7261 aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat caataccgta
     7321 ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc tggccagctt
     7381 tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc ggcggtgttc
     7441 gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat cagtgccacg
     7501 ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata gttactttca
     7561 aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa ggcgtagcgg
     7621 cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt cgcgcccgcg
     7681 acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac ttccagcacc
     7741 cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga ttccataaca
     7801 gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc gtcgaggatg
     7861 gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt cgactggatt
     7921 tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc gacgccgcgc
     7981 ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat cagcattgca
     8041 acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc ttttgccaga
     8101 attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat gttgccgccc
     8161 tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc gagccgtttt
     8221 tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag cgggtcgata
     8281 atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt cagttctacc
     8341 tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact aaagacatat
     8401 tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc gttacgttgt
     8461 aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc gctggaacgc
     8521 tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa ttgatcaacc
     8581 ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt ttcccgctcc
     8641 acgcggccgg ttggcgcttg ttcggctgcc agtttaagca caggtggggt agctattttt
     8701 ggcgaaactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa acagagtacc
     8761 gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga atccgcccct
     8821 ttcaccacgt catttaacgt tgtcagatgc cccagctctt cttccagcag gtcgacttcg
     8881 ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact ttgctcatct
     8941 tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt ttcgcctttc
     9001 gcttctaatg ccagttgacg caaggcctgg cagatataat cgtagctggc ggcatccggc
     9061 tcttgcgact gtttataagc gtcgagctgt tcttgcatga tgtccttcgt ttccaaaaac
     9121 agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc gagcaggttt
     9181 tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc ccctcctttg
     9241 atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc ttccggctgc
     9301 aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc aaaaaatgtc
     9361 tgataaaaat cgcttatatc gatgctcacg ctgtcacctc ggttcggctg atggcattgt
     9421 gggaacactg acctgtggtg caacctcagg tttttctagg gcgcttactg gctcattctg
     9481 gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta ccaacaggct
     9541 gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg ttgccgccat
     9601 gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt cgcggcggga
     9661 tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac cgctggcgta
     9721 gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt tcagcacagg
     9781 cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc cggttctaaa
     9841 catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga ccagatcgat
     9901 tttgagatgg agacgtaacg cccgcagttt cggatcggac tctatcaact ggtcgagatc
     9961 accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt cgatgttcgg
    10021 ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg gaattgggct
    10081 ttcactattg gaaatgcgat cgccgcccgt aaccgctgtc gccagtggag tccggaagta
    10141 ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca tcaccagaaa
    10201 aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc catgtgctgc
    10261 cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt gattcttcat
    10321 gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg ctcttccagt
    10381 tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc aacggcgata
    10441 ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg catcattttg
    10501 ctggtttcgg cgcttttctg acgtaaaaca ctcgctagtg gggaaataaa tccgtaagcc
    10561 aataaaatac cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc cagctcggcg
    10621 gcaggacgat cggctgaacc taacgcgtga acgcccccca ttacagccgc gacaatacca
    10681 aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc tgcctcgctt
    10741 tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt catgtgaccg
    10801 ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc cgcgaggatg
    10861 cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg ttccagcgaa
    10921 aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag cagatccata
    10981 tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt cagcgtgcct
    11041 ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc ggcaataatc
    11101 accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa ataaccgccg
    11161 aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc atccttccac
    11221 tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg gaaaaaaaaa
    11281 gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag ttaaacagcc
    11341 tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg ggaaagttta
    11401 cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct gccaacaggc
    11461 tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag ttgcagtaat
    11521 ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag tggtccttct
    11581 tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc atcgacgcca
    11641 ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga agcatgaacg
    11701 ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt cggcggtggg
    11761 cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg tgtttcgctt
    11821 tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg aatatcccgc
    11881 gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc agcgcttctt
    11941 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    12001 ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac
    12061 ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga aga
//
LOCUS       DAZSNO010000113        11934 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZSNO010000113 DAZSNO010000000
VERSION     DAZSNO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11934
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.113"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            210..980
                     /locus_tag="ACHWN2_002941"
     CDS             210..980
                     /locus_tag="ACHWN2_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR8932390.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            971..1444
                     /locus_tag="ACHWN2_002942"
     CDS             971..1444
                     /locus_tag="ACHWN2_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR8932391.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1551..2090
                     /gene="dnaT"
                     /locus_tag="ACHWN2_002943"
     CDS             1551..2090
                     /gene="dnaT"
                     /locus_tag="ACHWN2_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR8932392.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2093..2830
                     /gene="dnaC"
                     /locus_tag="ACHWN2_002944"
     CDS             2093..2830
                     /gene="dnaC"
                     /locus_tag="ACHWN2_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR8932393.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2879..3373
                     /gene="yjjA"
                     /locus_tag="ACHWN2_002945"
     CDS             2879..3373
                     /gene="yjjA"
                     /locus_tag="ACHWN2_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR8932394.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3613..5904
                     /gene="opgB"
                     /locus_tag="ACHWN2_002946"
     CDS             3613..5904
                     /gene="opgB"
                     /locus_tag="ACHWN2_002946"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR8932395.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6042..7064)
                     /gene="lgoD"
                     /locus_tag="ACHWN2_002947"
                     /pseudo
     CDS             complement(6042..7064)
                     /gene="lgoD"
                     /locus_tag="ACHWN2_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7203..8117
                     /locus_tag="ACHWN2_002948"
     CDS             7203..8117
                     /locus_tag="ACHWN2_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR8932396.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8332..9693
                     /locus_tag="ACHWN2_002949"
     CDS             8332..9693
                     /locus_tag="ACHWN2_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8932397.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9742..11406)
                     /gene="tsr"
                     /locus_tag="ACHWN2_002950"
     CDS             complement(9742..11406)
                     /gene="tsr"
                     /locus_tag="ACHWN2_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFR8932398.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11525..>11934)
                     /gene="hpaR"
                     /locus_tag="ACHWN2_002951"
     CDS             complement(11525..>11934)
                     /gene="hpaR"
                     /locus_tag="ACHWN2_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR8932399.1"
                     /translation="EAAMSYFRPIVKRHNLTEQQWRIVRILAESPSMDFHDLAYRACI
                     LRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRAQTQIEEAYRQIEA
                     QFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2902 a         3155 c         2902 g         2975 t
ORIGIN      
        1 acaataaatt cattatagtg ataatattca tgtgatagtt tgtttgcggc gagagataat
       61 tcgcttttta tcaccgagct tgtcccaata accaggatta atcagcgcaa ttttgatctt
      121 aacctaccgc aaaacaaccg tgcttcatga tacaaatgga ttctcattat ctgaacaata
      181 cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga gcaacagcga gccgtaacgc
      241 ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc gcgttggttg
      301 atgagctttc ctcacgactg ggtcgggcgc tgggaatgga cagcgtcgaa agttctatct
      361 cttcgaacgc catagtgctg acaactatta aagatgggca atgcctgaca tcgacacgta
      421 aaaatcacga tcgcggcatt aatatgcatg tggtgactga agtccagcac attgtgattc
      481 ttgcggagca tcatctgctg gattacaaag gcgtagagaa acgatttagc caaattcaac
      541 cattacgtta cccaagatgg ctggtagcct taatggttgg cctttcttgc gcctgtttct
      601 gtaaactcaa taacggtggc tgggatggtg ccgtcatcac cttctttgcc agtacgaccg
      661 cgatgtatat ccgccagctg ctggcacaac gtcatcttca tccacagatc aacttttgcc
      721 ttaccgcttt cgccgccacc accatttccg gattgctttt gcaactcccc actttcagca
      781 atacccccac cattgcgatg gccgccagcg ttctgctgct agtgccgggc tttccgttga
      841 ttaatgccgt cgccgatatg tttaaaggcc acatcaatac cggactggca cgctgggcga
      901 tcgccagtct gctgacactg gctacctgcg tcggcgtagt gatggcactg acgatttggg
      961 ggctacgcgg atgggtgtga tcgaatttct gttagcgttg gcgcaggata tgatcctcgc
     1021 cgccattcct gcggtcggct ttgcgatggt gttcaacgtt cccgtgcggg cgttacgctg
     1081 gtgtgcgctg cttggctcga taggtcatgg ttcccgaatg atcttgatga ccagcgggtt
     1141 gaatattgag tggtcaacct ttatggcttc tatgctggtc ggtaccattg gtattcaatg
     1201 gtcgcgctgg tatctggcgc atccgaaagt gtttaccgtg gcggccgtta tccctatgtt
     1261 cccgggcata tcggcttata ccgcaatgat ttcgtcggta aaaatcagcc agttaggtta
     1321 cagcgaaccg ttgatgatta ccctgttaac caactttctt acagcttcat cgattgttgg
     1381 tgcgttatcc atcggtcttt ccattcctgg attatggttg taccgcaagc gccctcgcgt
     1441 ataaaattgc ctcgcttaag cacacggatg agagacagcc tcctctcctc cgtgtgttac
     1501 tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca
     1561 gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc
     1621 tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg
     1681 ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagcta gcgcgtccgg
     1741 gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg
     1801 tacccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgat tttatccgcc
     1861 tggcggcgct atggggcgtg gcgctaagag agccggtgac caccgaagaa ctggcctcat
     1921 tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac
     1981 tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga
     2041 atacggtcag tgaacctgac agccaaattc caccaggatt cagagggtaa cgatgaaaaa
     2101 cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacctgcatt
     2161 caaaacgggt gaagagctac tggcctggca gaaagaacaa ggggcgatcc gctccgccgc
     2221 tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg
     2281 tccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa
     2341 tgcgttaagc aaagcgcgcc agtatgtcga agagtttgac ggcaacatcg ccagcttcat
     2401 cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct
     2461 gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa
     2521 agataccttc aggaatagcg gtaccagcga agaacaactg cttaacgatc tgagcaacgt
     2581 tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat
     2641 catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa
     2701 cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc gtatgcgcct
     2761 gggtaacagt ttatgggtga tcttcaactg ggatagctac cgtagccggg taacgggtaa
     2821 agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat
     2881 gatgaaaact attaaacatc ttctgtgctg tgccattgcc gccagcgcat taatttccac
     2941 cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa
     3001 ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag
     3061 cagcggaaac caagccttaa gcgcagataa catgaacaat gccgcaggca ttctgcaata
     3121 ctgcgcgaag caaaagctgg cttcggtaac cgatgccgaa aacatcaaga accaggtgct
     3181 ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc tggacggtat
     3241 tcagggtttg ctgaaaacaa aagatggtca gcaactcaat ctggataaca tcggaacgac
     3301 tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa
     3361 cttcatttcc tgaaccgcga aatgttaccc ggctgacgcg gctctctcct gcccttttcc
     3421 ctgctcctgg acggttttcc ccctgttaca cgggaagcga ccgcgatcaa aaaagtctgt
     3481 ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca gcaatatggc
     3541 gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg gctaaacggc ccggtttagt
     3601 gaggatcgcc cattgtcaga actactctct ttcgcccttt ttctcgcctc tgtgctgatt
     3661 tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacgttaac ggtgctgggg
     3721 ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg cgatggtatt
     3781 aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt cagcaaatac
     3841 atcctgccgg gtatcggcat tgtgctgggg ctgacggcgg tgttcggtgc gctgggctgg
     3901 atcctgcgcc gtcgtcgtca tcatccgcac cattttggtt acagcctgct ggcgctctta
     3961 ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc agataacgga actggtgaaa
     4021 tcccagtcac gcgatggcga cccggacttt gcggcttatt ataaagagcc gtcgaaaact
     4081 atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga gcggacctat
     4141 tttgataacg aagctttccc ggatctcacg cctgaactgg gcgcgttgaa aaatgaaggc
     4201 ctggatttca gccacacgca gcagctgcca ggaacggatt acacgattgc gggcatggtg
     4261 gcttctcagt gcggcatacc gctgttcgcc ccctttgaag gcaacgcctc cgcctctgtc
     4321 tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc gggttatcag
     4381 aactatttcg tgcagggcgc aaatctgcgt tttgccggta aagatgtgtt cctgaaatcg
     4441 cacggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtagc cgacccgcac
     4501 tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg gaaaaagttt
     4561 gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt cgatacccat
     4621 cacccggatg gttttatctc gcgcacctgt aaccgcaaaa aatatgattt tgacggtaag
     4681 ccgaatcagt cattcagcgc ggtaagttgc agccaggaga atatcgcgac gtttatcaac
     4741 aaaatcaaag cgtcaccgtg gtttaaagat actgtcatcg tcgtctcttc tgaccattta
     4801 gcgatgaaca acacggcgtg gaaatacctc aataagcagg atcgcaataa cctgtttttt
     4861 gtcattcgtg gcgacaagcc gcagcaaaag acgctggcag tgaagcgtaa cacgatggat
     4921 aacggcgcga cggtgctgga cattctcggt ggcgataact atctcggact tggtcgtagc
     4981 agtttatccg ggcagtcgat gtcggaaatc ttcctcaata tcaaagagaa aacattggcg
     5041 tggaagccag atatcatccg cctgtggaaa ttccctaaag agatgaaaga gttcaccatc
     5101 gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc gctgctgttg
     5161 cgggtttcag acaaacgcgt ggaaccgctg ccggaaagcg aatactcagc accgctgcgt
     5221 ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgacag ttgctacaag
     5281 atggcacaac tctgggctcc ggaactggca ctttccaccg actggtgtgt ctcgcagggg
     5341 cagcttggcg gtcagcaaat tgttcagcat gttgacaaaa caacatggaa gagcaagacg
     5401 gcatttaaag atacggtcat cgacatggcg cgttacaaag gcaatgtcga tacgctgaag
     5461 attgttgata acgatattcg ctacaaagcc gacagtttca tctttaacgt cgccggtgcg
     5521 ccagaagagg tgaaacagtt tagcgggatt tcccgtccgg agtcgtgggg ccgctggtcc
     5581 aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc gaagaaattt
     5641 gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat tccggtacgc
     5701 gtaggcaatg aagaacaaac tcttgtgctg ggcaatgaag tgaccaccac cacactgcat
     5761 ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc tgtctcaacc
     5821 aacgaaggga atatcctcgg acactcgccg cgtaagctcg ggatcggcat ggtggaaatt
     5881 aaagtggtag aacgtgaagg gtaattcctg actgatgtgt aggtcgcata gggtgcgacc
     5941 tacacgcctt ctccggctta caacacatta ttaagaaaca aaaaaaacag cgcccaacac
     6001 ttccccggac gctgcatggc aactttaata atctcgcctt atcagaaagt aattacaccc
     6061 ttaatcaact cacgattgtt aatcacatcg cgctcgtagg tttctgccag cgtggcgaac
     6121 ggatagcgat gggttaacat catgtcggca gtgattttcc cttccgccat cagtcgaccg
     6181 actttagcga aatcttccgg cgtggcgttg cggctgccca tcatcgtcgt ttctttttta
     6241 tggaattccg gatcggagaa ctgcaactca cctttaaaca gaccgacaaa taccaccgtg
     6301 ccgccgtgac gaatcagatt cacggtatta ttcatcgcat gttgattacc tgtcgcgtcg
     6361 atcactttct gcgccagcga accaccaaac tgcgcccgca gctgcgcgtc aaaatcttcg
     6421 gctgacgggt ccagcacggg caactccaga cgcgttgcca catgttcacg gcgcgccgga
     6481 ctggtatccg ccaccaccac ctgtgcgcca tcggctttag cgattgccgc cgcgcccaga
     6541 ccgattggcc ccgccccgac caccagcacc tgctcgccgg gagcaatggc tgcgcgacgc
     6601 accgcatgag cgctaatagc gaaaggttca atcaatgcgg ccgcctgcgg gtcaataccg
     6661 tctgcgggca aaatgttcgc caccggcacg ctcaaatact cactaaaacc gccatcctga
     6721 tgcacgccaa tgactgaaat tttttcacag cagttggtac gcccgctttt acacgccggg
     6781 cattgctgac aggcaacata agggatcaca gcaacttgct gaccattttt aagatcagca
     6841 atatttttac ccagcccaac aatctcccca catatttcat ggcctaaaac ccgtggataa
     6901 ctaaaaaatg gttgatttcc accctaggca tgaatatcgg taccgcaaat cccgacagac
     6961 ttaattttta ttaatgcttc attgtcaccc ggaataggta tctcgcgttg tttccagact
     7021 aattctttcg gctgctggca aattaaaaca ttcatcgtag acatgattcg gctatccatt
     7081 ttttgttgcc ttagatatca atgaaaatga aaaatggcag cggaacggaa aatctgtttt
     7141 gtgaaaacac acgcataaaa atgttttaaa tcgggtttta atggactcac aaggaggaaa
     7201 atatgagtcg ttcacaaaat ttacgccaca atgtgattaa ccaggtcatt gatgatatgg
     7261 cgcggggtca tatcccttcc ccacttccat cgcaaagtgc gctggcggaa atgtacaaca
     7321 tcagccgcac tacagtgcgt catattctca gccaattacg cgaatgcggc gtcttaacgc
     7381 aggttggaaa cgactatgtt attgcgcgta aacctgacca tgatgacggt ttcgcctgta
     7441 ctaccgcttc aatgagtgaa caaaacaaag tgtttgagca ggcttttttc acgatgataa
     7501 accagcgtca gttacgccct agggaaactt tttctgaact gcaactggct cgggccgcgg
     7561 gtgtcagccc ggtggttgta agagaatatc ttttaaaatt cggccgttat aatcttattc
     7621 aaagcgaaaa gcgcggccag tggagcatga agcaattcga tcaatcctac gccgagcagc
     7681 tgtttgagct acgcgaaatg ctcgaaacac attcacttca gcactttctc aacctgcctg
     7741 atcatgatcc ccgctggctg caggcgaaaa ccatgctaga acgtcatcgg ctactgcgtg
     7801 acaacatcgg caatagcttt cgcatgttct cgcaacttga cagagatttt cactccttac
     7861 tgctttcagc tgccgacaat atcttttttg atcaatcgct tgagatcatc tcagtcatct
     7921 ttcattttca ctatcaatgg gacgaaagcg atcttaaaca gcgcaatatc attgccgtcg
     7981 atgaacatat gaccattctc agcgctctga tttgccgcag tgaccttgat gccacccttg
     8041 cgctccgtaa ccatttgaat tcagctaaac aatcaatgat tcgatccatt aatgagaaca
     8101 cgcgttatgc tcattaagta cggattaaaa acagcaaact attagcgggt gcacggtttt
     8161 atcaaaagat aaataccggg aaattcccgc ttacctatgc tcacaatcag tcgactaaaa
     8221 atgtttttgt gagcggtagt aaagtcctaa aactttaacc tgccgttaat gtcaaccttg
     8281 cgtaatgcac aattacgtaa acaattaaaa ttataaatac aggagtccgg cgtggaaaaa
     8341 gaaaatatca ccctcgatcc gcgttcttca tttactccat cttcgtcggc agatattccc
     8401 gtgccaccag atggattagt tcaacgcagt acccgaatta aacgcattca aaccaccgcc
     8461 atgttgttat tattttttgc ggcggtaatc aattatctcg accgcagttc gctgtcggta
     8521 gcaaatttaa cgattcgtga agaattggga ttaagtgcca ccgaaatcgg cgctttgctc
     8581 tccgtgtttt cactcgctta cggcattgcg caacttcctt gcggcccact attggatcgt
     8641 aaaggcccac gcctgatgct gggactgggg atgttcttct ggtcactgtt ccaggccatg
     8701 tctggcatgg tgcacagctt tacgcagttc gtgttggtgc gtatcggtat ggggattggt
     8761 gaagcgccga tgaacccatg cggtgtaaaa gtcattaacg actggttcaa catcaaagag
     8821 cgcggacgcc cgatgggctt cttcaacgca gcttctacca ttggcgttgc tgtaagccca
     8881 ccgattctgg cggcaatgat gctggtgatg ggctggcgcg ggatgtttat taccattggt
     8941 gtactgggga tttttctcgc catcggctgg tatatgctct atcgcaaccg cgagcacgta
     9001 gaactgactg ccgttgaaca agcttatctc aatgcaggta gcgtcaatgc ccgccgagat
     9061 ccgctcaatt ttgccgaatg gcgcagcctg ttccgtaacc gcacaatgtg gggaatgatg
     9121 ctcggattca gtggcatcaa ctacactgcg tggctgtatc tggcctggct tcctggttac
     9181 ctgcaaacag cctataacct ggatttaaaa agcacagggt tgatggcggc tatccctttc
     9241 ctgtttgggg ctgccgggat gctggtcaac ggttacgtta ctgactggct ggtcaaaggg
     9301 ggaatggctc cgattaaaag ccgtaagatc tgcattatta ccgggatgtt ctgttctgcc
     9361 gcctttacgc tgatagtacc acaagcgaca acatccatga tggcggttct gctgattggc
     9421 atggcactgt tctgtattca ctttgccgga acatcctgct ggggcttgat ccacgtcgca
     9481 gttgcttctc gcatgactgc gtcggtgggc agtatccaga actttgccag cttcatctgc
     9541 gcctctttcg cgccgatcat tactggtttt attgttgata caacccactc attccgtctg
     9601 gcactaatca tttgcggttg cgtcaccgca gcgggagcac tggcgtacat cttcctggtt
     9661 cgccagccga tcaacgaccc gcgtaaagat taaactccct atgcccgata acattttgct
     9721 tatcgggcat ttttatggcg attaaaacgt ttcccagttc tccccgctat ctgccactgc
     9781 cattttacgc ggcgtagctg gcgtcacggt ttttaccaca gccgatgttt cacgctgctg
     9841 ttgctgctgt tgctgaatcc ggaacactgc cacagcttcg gtcaggcgac tggcctgctc
     9901 ttccagcgcg gcggcggcag cggcagattc ttccaccagc gcggcgttct gttgagttac
     9961 ccggtccatc tcagcaaccg ctaagccaac ctgatcgata ccacggctct gctcatcaga
    10021 agcagaagct atttcgccca taatgtccgt cacgcgggtc acagcactga caatctccgc
    10081 cattgtttcc ccggcgcttt cgaccagcgt agagccaaca tccactttcc ccaccgagtc
    10141 ttcaatcagg cttttaattt cacgaaccgc ctgggcgcta cgctgggcca gattacgcac
    10201 ttctcccgca accaccgcaa aaccacgtcc ttgctcaccc gcacgcgctg cttcaaccgc
    10261 cgcgttcaaa gccagaatat tggtctggaa ggtaatgccg tcaattacgc tgataatatc
    10321 ggcgattttc tgcgaactgg tggagatatc gcgcatagtc tgcaccacgt tatctaccac
    10381 tttgccgccg cgttgcgccg tttcagaagc acttaatgcc agatggctgg cctggcgcgc
    10441 attctcggcg ttctgtttca ccgttgcggt cagttgctcc atgctggctg ccgtctcttc
    10501 cagcgaagcg gcctgttgct cggtgcgcga agagagatca ttattgccgg tagcgatttc
    10561 gctggcaccg ctatagatgg cattggcccc gttgcgcaca tcaccgacgg tacgcatcag
    10621 ctctccctgc atatggcgca aactctctgc cagttgcccc atctcattag agccatccac
    10681 ctcaatcggt ttcaccagat cgccgcctgc aatatgacga atgctgtcaa tcaggcgatt
    10741 cattggcgct accagcgagg ctttaatacc gaaccagacg gcgaagatga ccgccagtac
    10801 gacgatcatc acgcccacca gaatccacat cgcctggctg taggaggcat tgttatcgct
    10861 gacggcgata tcatagagcc gatcgttttg ctccatgtaa gccacatact gcttctcgaa
    10921 accgtcctga tatccctggg tcggctgatc aaagaactcg ttgattttgc ctgcacctaa
    10981 cagttggatc agctccgcca gcgcattgtg ataaatatcg taattacgtt tgatctctgc
    11041 cgctgcggcg gtgctctgac gcgggtcacg cggcaacgct tcgtaatccg cccagttttt
    11101 ttccgcctgt ttcagcgaaa tactggcact ctgcatcagc tcagcaacgg ttgaaccgct
    11161 accaatatta ttctgatcca tcatgtagcg gatacccgcg cggttgaggg tgttacgcgt
    11221 ctgcaacaac gcgacccagc tgccattcag cgtgggttgc tgctggcgaa tggtttgtaa
    11281 aacagtgaaa ttttctttgt cattctttaa ggcattaaaa aacagaccgc ctgatgtcag
    11341 ttgtaaaagg ccaaaaacgg ccaaaaccag cagtaagctg gtcacgatct tgatacgttt
    11401 taacatggtt tctctttcct gtggaccgat gcaagatttt cggcctggaa agcaaaaact
    11461 ttatgaaaag gtgtaacccg ccatccatgg aaaaaccagg cgctggttat cagatactaa
    11521 aaagttattc attatcgccc gggatgtctt cttgtcggga attgcccaga gcaataaatt
    11581 cctctaacag atgggttaac tgttgcattt tctcggcagt aaactgagct tcaatctgcc
    11641 gataggcttc ttcaatctgt gtctgagcgc ggttatacag cgcctgcccc tctttggtca
    11701 gcgagatata gagcttccgc tgatcattga tcggctttaa tcgcaacact aaaccgtcgc
    11761 gttccatccg cgtgaggatc ccggtcaggc ttgggcgcaa aatgcaggcg cgatacgcca
    11821 gatcgtgaaa atccattgat gggctttccg ccagaatacg cacgatgcgc cactgttgct
    11881 cagtcaaatt atgccgttta acgattgggc gaaaataact catcgccgct tctc
//
LOCUS       DAZSNO010000114        11923 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZSNO010000114 DAZSNO010000000
VERSION     DAZSNO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.114"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..2250)
                     /locus_tag="ACHWN2_002952"
     CDS             complement(<1..2250)
                     /locus_tag="ACHWN2_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HFR8932400.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTEGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVE"
     gene            complement(2274..2897)
                     /locus_tag="ACHWN2_002953"
     CDS             complement(2274..2897)
                     /locus_tag="ACHWN2_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFR8932401.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2894..4582)
                     /locus_tag="ACHWN2_002954"
                     /pseudo
     CDS             complement(2894..4582)
                     /locus_tag="ACHWN2_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4731..7358)
                     /gene="gyrA"
                     /locus_tag="ACHWN2_002955"
     CDS             complement(4731..7358)
                     /gene="gyrA"
                     /locus_tag="ACHWN2_002955"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR8932402.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7505..8227
                     /gene="ubiG"
                     /locus_tag="ACHWN2_002956"
     CDS             7505..8227
                     /gene="ubiG"
                     /locus_tag="ACHWN2_002956"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR8932403.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8355..11923)
                     /gene="yfaL"
                     /locus_tag="ACHWN2_002957"
                     /pseudo
     CDS             complement(8355..11923)
                     /gene="yfaL"
                     /locus_tag="ACHWN2_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2756 a         3307 c         2945 g         2915 t
ORIGIN      
        1 ttcgacattg accagttcac caggctggta atgggttttg tccgttttca cccggatatc
       61 aagctgagga acggcaactt tgatcccggc gttctgaaaa ctgtactgac cattacgggt
      121 atacagcacc gaaaaagtga tgttaggcgc aaaggaattg ctcactggta cccgggcttc
      181 atacagggta tcgtttaaac gttgtagcgt tagccagttt gccggatgcg aaagcagcga
      241 ctgctgttcc acgcgatcgc gttccagcgt caataacgct tcatcaattg gctccgggaa
      301 agtaatcagc atcttcgcgg tttcgcctgg ctggtacagc gttttatccg ccacgatatc
      361 taccgtacca gtatgcgccg tgctgccctt accgctgacg gcatgactta acccagcgag
      421 aattaagccg tctttatcgc gtaatgtcag attgtagttg ccaggtttag cgaaattgac
      481 ggtaaaggat ttgccgcctg acggtagctc tccgctatgg ctcgtgcggt cttcgagacg
      541 caaccattca tacgtaacag gaacctgttt tgaagattcc agcgcggcat aacggaacac
      601 aaccgactcg ccgctattac tgtattgtgc ggcggtactt aaagagtaat gcgccaggcc
      661 gcgttcaatg aggatctctt tggtggtggt gacgcgatac gccgcgccgt cactggcgga
      721 gacggttaac aaatagcggc tcggtttatc ggcggcgggg agatttaacg ccacatgacc
      781 gctggcgtcg gacaccgttt cgctgccttc cagcgacacg gggaaacgtc cggcataacg
      841 caaatcgtta ccgaccattg ataattgctg agcgcgcaaa cttaactgca cgcgggcatt
      901 ttttaccggc tcgccatccg ggtagagcag ttgcagtttg ccgctgaccg cttcgccagt
      961 tttgaactct tttttgtcga gagctaaacc aatctcgaaa tgtggcttga tgtagtttgc
     1021 cacgcgaaaa ctgctgctat agacctgatt gcggtaagca agacgtaact cataacctcc
     1081 ggcaacggca ttttctggca ggcggaaact tccctgcccg ccattgcgcg catccagcgt
     1141 gacatcgacg gtttgcaaca gactgccgtt agcgtccagc accgaaagct tcgccggggc
     1201 gctgacgatg ggggatgaat gcaacggatc gcggaactcg cggcccatca ctttaacatc
     1261 gacacgatcg cctgcgcgat atagcgggcg atcggtaaaa atatacaagc gggtgttgta
     1321 gatttcgctt tcgtagaaga agttctcgga gacaaaaacg ccgccttcag catccttacc
     1381 cagaatgtat gaacgttctg gcgatatatg ttgtaactgc aaagtaccgc tgtcatcggt
     1441 caccccgcgg gtcatcacgc caagaccttc agtccacaag atctctgagc cgggcttcgc
     1501 ttcaccctgt tttttacccg cggtccatac cagaagctcg ttacctgaca ctttgctaag
     1561 cgccacggta tcggaaacaa acaccaccgt cgtcgcccga tacccaccta ccatcgcctc
     1621 gacgaggtac agccccggct cttgttggcc gagaggaata taaatattac ccggctgcgg
     1681 cgagatgaaa ttgctggatg cgccttccag ttttacccct tgctgcggct cgaccggttt
     1741 agcctgccat agtggataac ggaactgttc caccaaggga tattttttca gcggggaaaa
     1801 ctggttgttc tgtacataac gggaaggttt aataatggca ttgccgagat gtaattcggg
     1861 taatgcctga gtcacattct gccgtgactg agaagagaaa gtacgctgca tcacgcggcg
     1921 agatttgccg taccagttat cccacagcca ggtcagcgta ttgttcagcc cgtcgcccag
     1981 atattgcggt tgcaccacaa tgcgatgcag gtttttctgc tggcgcaaaa atgccatcgg
     2041 gtcaggaata cgatataggc gaacgtccac gccgccgtac tcttccatct gatagcgccg
     2101 ataatcacgc cccggcgctt ccagtcgcac tttcgcctct tcactgctgc taaaactgct
     2161 gtcagcaagc aaaaagaatg ttcccccggc gggcggcgca tagttactgg aaggaagcga
     2221 atcatcagct ttagcaagcc ctgttcccac aagagacagg gacaagcacg ctgctatcgc
     2281 gccagaaaat ttaaacgata aatgccaatg aaattgggat tggaatcgtt gggtatccat
     2341 cgggtgtcct tccatgtcat aagttgttgc agactgactg cgcgcattcc gttgtcagtt
     2401 ttcgtggcgc ttccggtgtg gtagatgacg taacgcccca tccagaccat taagtgctgg
     2461 gcatcgccct gatcgaaaaa aatcatatcg ccaggcagcg cctggtttat gtcttggcca
     2521 ataaactggc tgttgtactg aatcaaatta atcgcggtca cgtaggggcc gtttttcccg
     2581 ctcccctgat tccagttttg cgccagttga cgctgttcag gtgttagcgt catctctggc
     2641 ggcaaatact ggctagataa accgttactt tttagccatt tactgtcgtg aactttcagc
     2701 gtctcgttcg ccgcaaatcg caccaggccc gcacaatcct gctgatacca gcgtggactt
     2761 ggcccctggc ggagctgttc ttgtgcaatg cgtacaaacc aggcgcgaaa cagcctggat
     2821 tgttcgacat tcagcatttc gctatgggca acaacacaac acagccagca aatcagcgcc
     2881 agcagcccgt gtctcatagc ggctgccagg ttatgggtag ccactgccag gcgacaccgg
     2941 gttccatctg ggccagcttc atgacataac gcggttgttg agataaagcg tccagcttcg
     3001 gcattaataa agtttgtgcg gcgttataaa aaaccggttc gagattcttc ggcagactgg
     3061 tcaacgtttc gttacgcagc agtttcgcca cgccttgtgg attgatatag agcggaacga
     3121 tgccatcagt gggtattaca tccaccattg ccgggcgggt tttattcagt gtttgcaggg
     3181 cgttattaac cagcgtgtca tcgagggaga aaagcagcgt tttgttttgc atcgccagcg
     3241 acacacggaa aaaataatcc gacattaatt gatcgggttg cgccgcctgc gctttcggat
     3301 actgaccgta tcgggaactc acttcgcgac accagatttg cgcttcgccc tgctgagtct
     3361 ggcttaccgg caaaacacct tctggcgctt tgctttcgtg cgcaccaata ttttgcgtaa
     3421 acagtttccc tggcaattgc gcctgttcgg cagtgccatc aaactgaccg acaaacagcg
     3481 gggtttgcaa ttttgagtct tcataccagc acagccccgc ggcaccgtct aacgccccat
     3541 tcagcttgtc gttttcctgg ctgatgtgcg aaagcatctc ttcggcaata ccgtgtgaat
     3601 acggcaccgc cacacagaag ctggctccgg caggcatact gttccagacc ggcgtaaaat
     3661 cgaaactggc atctacgctg gcggattcat catttaacgc cacaaaactg tgccagccgt
     3721 cgttacccat ttcgaagcgt acgccagcaa aagaaggcat taaccgctgg tagccaaaac
     3781 ccagcaacct ggcgctgacc acgatgcgca ggcgtactgg cgttttttcg gctgtacgct
     3841 cttccaggcc aaagctggct tgccagcgtt ttttaccgct caacaaatca cttgcgatcg
     3901 ccgtggcttc ggtatcctgc tgatcatctt taaacaacat atccgtactg gaaaacacca
     3961 gcatcttgtc ctgatatgtc gcgaacatca gggcgttatt gccgttatag cgcaactgat
     4021 aaacaggaat agtttcacta tttatcttga tgctactgat ttccgttttg cttaactagc
     4081 tgtcgctggt agcggcaaac aacaatggct ccagcagttt gcttaaacca ctgcgctgga
     4141 tcagcaccat gtaatgtgaa agatggcctt gtttatcgtg ccatagcgcc gcctgcgcgg
     4201 gctgatctaa gagtgacgaa aagagcttat ctttcagcgt gagatcgtgt tcatagacaa
     4261 tgcggcgaat gctgccttca atgcccagac gatcggcatg attctgataa tagaaaacga
     4321 aatcttcgct cagaacatcg tggagaaacg gaatggtgag gagatctttg ggaagctggc
     4381 tcagagagtc gctgtctaga aagaggtccg gctcattgag atcgatttgc agattgttgt
     4441 gcaccaccag cggcgacaac gttttttctg gcccactgcc agcatattgc aacgcccaga
     4501 cgccagcgga aagcagtgct attgcgccaa aacctacaag accatagaac cgccagcctt
     4561 tcgccttttt ttcaccactc attgccacat tccttgtgta tagccagcca ttttttacgg
     4621 gcacagccaa actttaccgc gccctaatac gacaaaagcc cagactttgc agcctggact
     4681 tttcaattca aacaagggag atagctccct tttggcatga agaagtaaaa ttattcttct
     4741 tctggctcgt cgtcaacgtc cacttccgga gcaatttcat catccccttc cgcggcactg
     4801 ccgtcgatgg tatccagatc ttcctcgtca accggttcag caacacgctg cagacccact
     4861 acgttttcat cttccgcagt acggatgagg atcacgccct gggtgttacg gcccacgatg
     4921 ctgatttctg aaacgcgagt acgtaccagc gtaccggcat cggtgatcat catgatctgg
     4981 tcgcagtcat ctacctgcac cgcgccaacc actaaaccgt tacgttcggt aaccttgatg
     5041 gagataaccc ctttcgtcgc acgcgacttg gttgggtatt ccgccactgc agtacgttta
     5101 ccgtaaccgt tttgcgttgc ggtgaggatt gcgccatcgc cacgaggcac gatcagagag
     5161 acgactttat cgccttcacc taagcgaata ccgcgcacac cagtggtatt gcagcccatc
     5221 gcacggacag aagactcttt aaagcgcacc actttacctt cggcggagaa cagcattact
     5281 tcatcttcgc cactggtcag atcaacgccg atcagctcat cgccgtcaac cagtttgatc
     5341 gccactttac cggcggtacg cagacggttg aattcggtga ggacggtttt cttcacagta
     5401 ccgttagcgg tcgccatgaa gactttcacg ccttcttcaa actcggtcac cggcaggatc
     5461 gcggtgatac gttcgtcctg ttccagcggt agcaggttga cgatcggacg accgcgcgcg
     5521 ccacgagtgg cttccggcaa ctggtaaact ttcatcgaat agacgcgacc acggctggag
     5581 aagcacagaa tatggtcgtg cgtgttcgcc accagcagtc ggtcgataaa gtcttcttct
     5641 ttaatacgtg cggcagattt acctttcccg ccacgacgct gcgcttcgta ttcagaaagc
     5701 ggctgatatt taacgtagcc ctggtgagag agcgtcacga ccacatcttc ctgggtgatc
     5761 agatcttcca ggttgatgtc tgcgctgttg gcggtgattt cagtacgacg tttgtcaccg
     5821 aactgttcac gaaccagctc cagctcttcg cggatcactt ccatcagacg atcggcgcta
     5881 ccaagaatac gcaacagttc cgcgatctga tccagcagct ctttgtattc gtcgagcagt
     5941 ttttcgtgct caaggccggt cagtttctgc aaacgcagat ccagaatcgc ctgagcttgc
     6001 tgttcggtca ggtagtacag accatcacgc acgccgaact ctggctccag ccattccgga
     6061 cgcgcagcat cgtcgccagc acgttccaac atggcggcaa cgttgcccag ctgccacgga
     6121 ttagcaacca gcgcagtttt cgcttctgca ggcgtcggcg catgacggat cagttcgatg
     6181 atcgggtcaa tgttcgccag cgccacggct aatgcttcaa ggatatgagc acgatcgcga
     6241 gctttacgca gttcgaaaat agtacgacgg gtcaccactt cacggcggtg acgaacaaac
     6301 gccgcgatga tgtctttcag gttcatgatc ttcggctgac catggtgcaa tgccaccatg
     6361 ttgataccga aagaaacctg caactgggtc tgggagtaga ggttgttgag cacaacttca
     6421 ccgaccgcat cgcgtttcac ttcaatcacg atgcgcatac cgtctttgtc agactcgtca
     6481 cgcagcgcgc tgatgccttc cacgcgtttt tcttttacca gttccgcaat cttctcgatc
     6541 aggcgcgctt tgtttacctg atacggaatt tcgtggacga taatggtttc acgtccggtt
     6601 ttggcgtcaa cttccacttc agcacgggcg cggatatata ccttgccgcg accggtacgg
     6661 taagcttctt caataccgcg acgaccgtta atgattgccg ccgtcgggaa gtccggcccc
     6721 gggatgtgtt ccatcagccc ttcaatgctg atgtcttcat catcgatata cgccagacaa
     6781 ccgttgatga cttccgtcag gttgtgcggc gggatgttgg ttgccatacc tacggcgata
     6841 ccggaagaac cgttcaccag caggttagga attttggttg gcatgacgtc gggaattttt
     6901 tccgtgccgt catagttatc aacgaaatcg accgtctctt tttcgagatc ggccatcagt
     6961 tcatgggcaa ttttcgccag acggatttcc gtataacgca ttgccgccgc agagtcgccg
     7021 tcgatggaac cgaagttacc ctgaccgtct accagcatgt aacgcagcga gaatggctgc
     7081 gccatacgga cgatcgtgtc ataaaccgcc gagtcaccat ggggatggta tttaccgatt
     7141 acgtcaccaa cgacacgggc agatttttta taggctttgt tccagtcatt gcctagtacg
     7201 ttcatggcgt aaagtacgcg acggtgtacc ggcttcaggc catctcggac atctggcagc
     7261 gcacggccaa caatgaccga catcgcataa tccagatagg agctctttag ctcttcctca
     7321 atgttgaccg gtgtaatttc tctcgcaagg tcgctcatct aaccgctatc cctctactgt
     7381 atcccggatt caaaggtcgc aaattataac acagccgcgc agtttgaggt aaacctatac
     7441 gctttattca catccaatgc ctgatatact cgtttgtctt gccaattacg gagtagaagt
     7501 gccaatgaat gccgaaaaat cgccggaaaa ccataacgta gaccacgaag agatcgctaa
     7561 atttgaagcc gtcgcctccc gctggtggga tcttgaaggt gagttcaaac cgttgcaccg
     7621 cattaacccg ctgcgtctgg gctatattgc cgagcgtgct ggcggtttat ttggcaaaaa
     7681 ggtgctcgat gtcggttgtg gcggcggcat tctggccgag agtatggcgc gcgaaggcgc
     7741 gacggtgacc ggtctggata tgggctttga gccattgcag gtggcaaagc tgcacgcgct
     7801 ggaaagcggc attcaggtgg attacgtgca ggaaaccgtg gaagagcacg cggcaaaaca
     7861 tgccgggcag tatgatgtgg tgacctgcat ggagatgctg gagcacgtac ccgatccgca
     7921 gtcagtggtc agagcctgtg cgcaactggt gaaaccaggc ggcgatgtct ttttctcgac
     7981 gcttaaccgc aacggcaagt catggctgat ggcggtggtt ggcgcggaat atattttgcg
     8041 catggtgccc aaaggtacgc atgatgtgaa gaagtttatt aaaccggcag agctgcttgg
     8101 ctgggtggat cagaccagtt taaaagagcg acatataacc gggctacatt acaacccgat
     8161 cactaattcc tttaagctcg gccccggcgt ggatgtgaac tatatgctgc acacgcagaa
     8221 taagtgaggt tgatgtttgg ccgcgccaat gcctgatgcg acgcttgcag cgtcttatca
     8281 ggcctacaaa tgctctccgt aggccagata aggcgtttac gccgcatccg gcaattgtga
     8341 tggtcagaca gcgattacca tttcaccgtc atcgacaaaa accctgccgt ctgggcaaaa
     8401 tcatcactcc ctttctgcca cgccacgctg ccgcgcaggg acactcgctg actgatattg
     8461 cccgtgactc ccacttttat ttcaccccgt agcttcaccg catcgtcact gatagtgctg
     8521 ccatcttctt caatttccgt cgcatgggga tcgtgatagt aattcagatc taatgttggt
     8581 gtgacaccaa ccgcggtacg ccattcgctg tgtaaaccca gccgcgtctg aatatcatcc
     8641 ccctgcgatt gtgacacgcg cgcatggtta gcggcggtaa aatcatcctg ctgcacgccc
     8701 tgataaatca cctgcgcctg cggttcaatc accacaccac gccccggtaa ccactgatac
     8761 ccagcttcca gcgaggcgat aatccccgac gaatggtaac gatccacgcc atcttcatgt
     8821 tcggaaacat cattgttaaa ccacgcgtat tgcagccagc tatccagcca ggccccctgc
     8881 ttctgattac cgtgctgata ccagcttgag gtcagcccaa cggcataacc gtggttctgg
     8941 ttatcggcgc gagttccggt catattcgag cggctgtcgc cctgattatc actgtagcca
     9001 ccaaccgccc caagcatcca ctcgccatcc gtgccccagc gcccgctaaa caagccgccg
     9061 ctaagctgca ccgtagaagt gtcttcctgc tgagccagtt gccctgccgc cgtgtaatga
     9121 taacgtccgc cgataacacg taaattcagc gtctgaccat cgccaccagc gtgatcgcgt
     9181 cgctccatca taaacgcctg atttgccgcc cgcaggttat tgagataacc gccaactttg
     9241 gcattcagca ccggctggta agcaggcgta ggggccgggt cgggggttgg gtcaggatcc
     9301 ggcgtgggat caggatcggg agtcgggtct ggctcaggtg gcgatggcgg cgttacttct
     9361 tgcgatcgca gataccagtc gttgttatct tccactagcg tgtagtcata cgctcccatg
     9421 ttgacgtagc cactgccagc cagactgaac tgtgcattgt tttgaaactg cgtgggatca
     9481 gctgcgaaat caaccacttt aatgcctgtc gatgtcggct caccaatccc tgtaatggag
     9541 ttaaccacca cagttgtgtt gccagcagta ttaccgttca tcaccaattg atcgcttacc
     9601 gagtcatcgc cgtttaattc gctgtcgaat agtagcgtac cgccgccggt ataatcaccg
     9661 tttaccgtca gcgtatcgcc agcgacgcca ttttgcaggc taaccgcacc ggacgtattg
     9721 gtcagatcac cattaaccgt cacgtcagca agataggtca ggctgtcagt ttcaaggttg
     9781 gtattaagct cgcctgtcaa cgtcacgcca tcggtagcat ttaccagcgt cccatcgccg
     9841 ttaaatagtg aggcattaag ggcgacggaa gtatcctgcc cggtcagacg caaaaccgta
     9901 ccatcgctga tgtcgatagt gccgctggaa gtagcatcaa ttgactgaat atcctgatcc
     9961 gcgccagtaa cgaacgttgc cccatcacca acccacagtg acgaagcata aggaaagata
    10021 tccgcaacat cgcctttcag ggtgccttct tcgacacgta ccgccgactg cgttgtaccg
    10081 ctggcggtca gctccagcgt acccgccccc gttttagtaa atgtgctacc ctcatcctga
    10141 tgctgcccgc tgctgtcagc catcagtgcg ccccactgcg tgtctacccc cgcatcaact
    10201 gccacttcac cgtcggcgcg catttcaata tcacgcccat gctcagtcgt cgtagagcga
    10261 gtatcgacat tggcagtaat ctccatcacg ctttcttgtt acttatcggt aaagataacc
    10321 tggcggttat agtgggtatc tccaagctgc gagttgtcgc tcaccatcag cgtaccggag
    10381 gcgatttgtg ttgtgcccaa ataactatta ttgtttgcca gacttacctg cccgtcgctc
    10441 gcatcgacca ccacgtagac gccatctttc ccgtcgccgc cgatatgcaa attgtcgcca
    10501 cctgcaagat ctaccacatc ctgactgccg ataaccgttc cgctgctgcc actgacttca
    10561 aggccatcat tgtatgatgt gccgctctgc caagtggaga aatcagagag atcgagcaca
    10621 ccgccgttta acacgatcga ctgcggatcg tcctggagag cggtaagatc tgccgcgtcg
    10681 ccttccagcg aaagcaccgc accatcatca acgactatat cgccggttag cgccatcgac
    10741 tgcgcccctg ccagcacgta gctgccgttt tgcgcaatgg tgagctgacc agcaccttcg
    10801 atgatgccag caaaactgcc ctgattaaca gtaacgttgc caccagcatc gatatttaaa
    10861 gtgccattct gaaagcccgt caatgcgtgc acaaaagttt gttgggtcga gccaacgttt
    10921 agctcagcct gattctgata ctgatcaata ctccctatcg tcagaccgta gcagtcttgc
    10981 ggatcgtcct ggcaatgcgt atcgccgaca ttcatcaggg agttgctgcg acccagggta
    11041 acttcaccgt tttcaatctg catctcacca gtaaagtcat tgttatctgc attaagtacc
    11101 agatcgccgg aacctgtttt ggtgattaac ccggtaccag caatagagtc aacagctccg
    11161 tcattctctg tattgccgat aaccagcgtt tttccgtcgg caatatcaaa ggtaacttca
    11221 cttaagccga gatacataaa gccacccgcc gcagtggagg gaccatctcc atagcctgct
    11281 gcgctattgt tctcatcgac taacacgccg ccgttctggc tgtagctgtc atcaacagaa
    11341 atatcaataa gatagggagc cgtcgcgcta ttggtatata tcgccccgcc ataaccttcg
    11401 gctgtgttat ttgtaaaggc ggtgttattt attatcgtat aacctgaagg atgcttgctg
    11461 tcgctattat tatcggtaac atcgattgcc ccgccatcgc catcactgta acttgttgat
    11521 gtatatgcct ggttattgtt aaaaacatca tcacttaaat agacgtcatt attaatggta
    11581 taaattgcgc cacctttgcc atcattagcg atattattga tggcgttagt gacacgtaaa
    11641 tcgacggcac cagtatcatt agtaccagaa gaatagattg cgccaccata gccgcctgcg
    11701 acgttaccgg aaaaaataac atcagtaaga ttcagcgttg agttttcttt agcaaatatg
    11761 gccccgccgt tattatattc tcctgtaacg gtgttattgg caaacagagt cattccagtt
    11821 tcattttctg gtaatagtga aaattccgct ccttgttgca aaaatacggc cccaccgctg
    11881 gcattattgg tcatatccga aaaaaccagc cattgcccat cgg
//
LOCUS       DAZSNO010000115        11857 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZSNO010000115 DAZSNO010000000
VERSION     DAZSNO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11857
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.115"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(92..832)
                     /gene="cysQ"
                     /locus_tag="ACHWN2_002958"
     CDS             complement(92..832)
                     /gene="cysQ"
                     /locus_tag="ACHWN2_002958"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR8932404.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            1022..2965
                     /gene="cpdB"
                     /locus_tag="ACHWN2_002959"
     CDS             1022..2965
                     /gene="cpdB"
                     /locus_tag="ACHWN2_002959"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR8932405.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3091..3471)
                     /locus_tag="ACHWN2_002960"
     CDS             complement(3091..3471)
                     /locus_tag="ACHWN2_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR8932406.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3560..4420
                     /gene="qorB"
                     /locus_tag="ACHWN2_002961"
     CDS             3560..4420
                     /gene="qorB"
                     /locus_tag="ACHWN2_002961"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR8932407.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            4529..5494
                     /locus_tag="ACHWN2_002962"
     CDS             4529..5494
                     /locus_tag="ACHWN2_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR8932408.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5602..6264
                     /gene="ytfE"
                     /locus_tag="ACHWN2_002963"
     CDS             5602..6264
                     /gene="ytfE"
                     /locus_tag="ACHWN2_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR8932409.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6309..7721)
                     /gene="cycA"
                     /locus_tag="ACHWN2_002964"
                     /gene_synonym="dagA"
     CDS             complement(6309..7721)
                     /gene="cycA"
                     /locus_tag="ACHWN2_002964"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR8932410.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(8029..8649)
                     /gene="fklB"
                     /locus_tag="ACHWN2_002965"
     CDS             complement(8029..8649)
                     /gene="fklB"
                     /locus_tag="ACHWN2_002965"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR8932411.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8868..9413
                     /locus_tag="ACHWN2_002966"
     CDS             8868..9413
                     /locus_tag="ACHWN2_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HFR8932412.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(9397..10044)
                     /locus_tag="ACHWN2_002967"
     CDS             complement(9397..10044)
                     /locus_tag="ACHWN2_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR8932413.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            10256..11047
                     /locus_tag="ACHWN2_002968"
     CDS             10256..11047
                     /locus_tag="ACHWN2_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFR8932414.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            11057..>11857
                     /locus_tag="ACHWN2_002969"
     CDS             11057..>11857
                     /locus_tag="ACHWN2_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR8932415.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINS"
BASE COUNT         2995 a         3078 c         2988 g         2796 t
ORIGIN      
        1 ttaaatacaa tctcttttta tttataactt tttaggttgg gataaatcag gatggcagaa
       61 tcaggaaata cactgtttct gccatctgaa tttagtaaat ggacactctg aaccccggat
      121 tcaggaacga ctcgcgcgga gtgtaatcca gcggtttacc ctgccagtcg tgaacgtgcg
      181 ctccggcagc tgcagccaca gcatgtccgg cggcggtgtc ccaaatattc gttggtccga
      241 agcgcgggta cagctgcgcc tgtccttccg ccaccaggca gaatttcagc gaagagccga
      301 tggacgtggt ctgatgttcg ccaagctgtt gcagatactc tttcagctcc gcatctgcat
      361 gggaacggct gataaccacc agcggcgggc gcgcatcgcg gacctgaatc agcttgcgca
      421 caccgcactc ttctttccag gccttgcctt ctgccgcgct gtacattacg ttcattaccg
      481 gcgcatacac cacacctaaa atcggtttgc catggtcaat gagcgcaatg ttaacggtga
      541 attcgccatt acgtttaata aactctttag taccatccag cgggtctacc agccagtaac
      601 gctgccagtg ctgacggact tcccaaccgg gaggatcttc ttcagaaagg accggaatat
      661 ccggtgccag cgtacgtaaa ccgtccatga taacggtgtg agcggcaata tccgctgccg
      721 ttaccggaga attgtccgct ttgctgacga cgtccatcgg tttcgtcccg tcgtagacct
      781 gcataatggc atcgcctgca ttccgtgcaa gctggcatac ttgatctaac atttctccac
      841 ctcgtctctg tgagcggtgt taacttattg ttttacttat accctatcgt taatgaatgc
      901 gccaactgtg atagtgtcat cattttcaaa gcgtaaaatt gtggcattct tcactgttct
      961 ataagtaagg cgtttattct cccttttctt tcgtattccc gatgataaaa ggatgtccct
     1021 gatgattaag tttagcgcaa cgctcctggc cacgctgatt gccgccagtg tgaatgcagc
     1081 gacggtcgat ctgcgtatca tggaaaccac tgatctgcat agcaacatga tggatttcga
     1141 ttattacaaa gacaccgcca cggaaaaatt cggactggta cgtacggcaa gcctgattaa
     1201 cgatgcccgc aatgaagtga aaaacagcgt actggtcgat aacggcgatt tgattcaggg
     1261 gagtccgctg gccgattaca tgtcggcgaa aggattaaaa gcaggtgatg ttcatccggt
     1321 ttataaggcg ctgaatacgc tggattatac ggtcggtaca ctcggcaatc atgaatttaa
     1381 ctacggtctg gattacctga aaaatgcgct ggcgggagcg aaattccctt atgtaaatgc
     1441 caacgtcatt gacgccagaa ccaaacagcc aatgtttaca ccgtatttaa ttaaagatac
     1501 cgaagtggtc gataaagacg gaaaaaaaca gacgctgaag attggctata ttggcgtcgt
     1561 gccgccgcaa atcatgggct gggataaagc taatttatcc ggaaaagtga cggtgaatga
     1621 tattaccgaa accgtgcgca aatacgtgcc tgaaatgcgc gagaaaggtg ccgatgtcat
     1681 tgtcgttctg gcgcattccg ggctgtctgc cgatccgtat aaagtaatgg cggaaaactc
     1741 agtttattac ctcagtgaaa ttccgggcgt taacgccatt atgtttggcc atgctcacgc
     1801 cgttttcccg ggtaaagatt ttgctgatat cgaaggggct gatatcacca aaggcacgct
     1861 gaatggtgtt ccggcggtaa tgccgggcat gtggggcgat catcttgggg tggtcgactt
     1921 acaactcagt aatgacagcg gtaaatggca ggtgacgcag gcgaaagcgg aagcacggcc
     1981 gatttacgac atcgccaata aaaaatccct cgcggcggaa gacagcaagc tggtagaaac
     2041 actcaaagcc gatcacgatg ccacacgcca gttcgtcagc aagccaatcg gtaaatctgc
     2101 cgacaatatg tatagctatc tggcgctggt gcaggacgat ccgaccgtgc aagtcgtgaa
     2161 caacgcgcaa aaagcgtatg tcgaacatta cattcagggc gatccggatc tggcaaaact
     2221 gccggtgctt tcagctgccg caccgtttaa agttggtggt cgcaaaaatg acccggcaag
     2281 ctatgtggag gtggaaaaag gccagttgac cttccgtaat gccgccgatc tttatctcta
     2341 ccccaatacg ctgattgtag tgaaagccag cggtaaagag gtgaaagagt ggctggagtg
     2401 ctccgccgga cagtttaacc agattgctcc taacagcacg aaaccacagt cactcatcaa
     2461 ctgggatggt ttccgcactt ataactttga tgttattgat ggtgtgaatt atcagattga
     2521 tgttacccaa cccgcccgct atgacggcga gtgccagatg attaatgcca atgcagagcg
     2581 gattaagaac ctgaccttta atggcaagcc gattgatccg aacgctatgt tcctggttgc
     2641 caccaataac tatcgcgctt acggcggcaa atttgccggt acgggcgaca gccatatcgc
     2701 ttttgcttca ccggatgaga accgctcggt gctggcagcg tggattgctg atgagtcgaa
     2761 acgtgcgggg gaaattcatc cggcggcaga taacaactgg cgtttagcac cgatagctgc
     2821 cgataagaaa ctggatatcc gtttcgaaac ttccccgtca gataaagccg cagcgtttat
     2881 taaagagaaa gggcagtatc cgatgaataa agtcgcgacc gatgatatcg ggtttgcgat
     2941 ttatcaggtg gatttgagta agtaaaacac ttctttttcg gcctataaat caaccgcatc
     3001 cggcatttat tggcggatgc gatgctgccg tatcttatcc accctacaag ccatgaaccg
     3061 tagaccggat aagcgcagcg catccggcag ttatgccgca cgttcatccc gcaccgccaa
     3121 cacctcgggc aaattcaact caatccagtc cgccagtgca gcaacctttt cgctcacctg
     3181 ctcgcccagc ggcgtgaggc tatattccac atgcggcggc accaccggat acgcgatacg
     3241 gttaagaaaa ccatcctgtt ctaacgcctg taacgactgc gcaagcatct tttcactcac
     3301 cccaccgatt ttgcgccgca ggtcgctaaa gcgatgagta ccttcgcgta gcgccaccag
     3361 aatcaacacc ccccaacggc tggtgacgtg tttcaacacc tcgcgcgacg ggcactgttc
     3421 cgcaaagaga ttaccctctt tcagttgttg tgacaggcta acctgactca tttcatactt
     3481 acctttttgc acgtacttac taaaagtaag tttaggtgtt agcgtattta aacacaagac
     3541 aaaacgatgg agacttccca tgatcgctat tactggtgcc actggccaac ttggtcacta
     3601 tattattgaa tccttgatga aaacggttcc tgccagccaa atagtggcta tcgttcgtaa
     3661 tccggcaaaa gcccaggccc tggcagcaca aggcattacc gtgcgtcagg ctgactacag
     3721 cgatgaagcc gcactgacat ctgcacttca gggagtggaa aaactactgc tgatctcttc
     3781 cagcgaagtg ggtcaacgtg ccccgcagca tcgtaatgtt attaatgccg caaaggcggc
     3841 tggcgtgaaa tttatcgctt ataccagcct gctacatgca gatacctccc cgctcggcct
     3901 cgccgatgag cacatcgaga cggagaaaat gctggctgat tctggcatcg tttacaccct
     3961 gctgcgcaac ggctggtaca gcgaaaacta cctcgccagc gccccggcag cactggaaca
     4021 cggcgtattt atcggtgcgg ctggcgatgg caaaatcgcc tcagcaacgc gggcagatta
     4081 tgcgacagct gcggcacgcg tgattagcga agccggtcac gaaggcaagg tttacgaact
     4141 ggcgggcgat agtgcctgga cgttgacaca gttagcggca gagctgacca aacagagcgg
     4201 caaacaggtt acctatcaaa atctgagcga agccgatttc gccgcggcac tgaaaagcgt
     4261 cggactgccc gacggactgg cggatatgct ggcggattct gacgttggcg catcgaaagg
     4321 tggtctgttt gataacagca aaacgcttag caaattgatt ggctgcccaa cgacaacgtt
     4381 agccgaaagc gtaagccatc tttttaatgt taataactag ttaattaaag tggcatcctc
     4441 ccgcatcctc tctgataatg acgggatgcc gggagcaatc atgtctgctt cctgaacgtt
     4501 tcttctgaca gaccaatgga tgccagtaat gattagcggc gtgctgtacg ccctgttagc
     4561 agggttgatg tgggggctta tttttgtcgg gccgttgatc gtgccggaat acccggcgat
     4621 gttgcagtcg atggggcgtt atctggcgtt agggttaatt gcgctaccca ttgcctggct
     4681 gggacgcgtg cgtctgcgtc agttggcgcg tcgggactgg cttaccgcct tgatgctcac
     4741 tatgatgggc aacctcattt attacttctg ccttgccagt gccattcaac gtactggcgc
     4801 gcctgtttcc acgatgatta tcggcaccct gccggtggtg attcctgtct ttgccaatct
     4861 gctttatagc cagcgcgacg gcaaactcgc gtggggaaaa ctcgccccgg cactgatttg
     4921 tattggcatc ggcctggcga gtgtgaatat tgctgagtta aaccacggac tccccgattt
     4981 tgactgggca cgttatacct ctggcatcgt gctagcgtta gtttccgtgg tctgctgggc
     5041 atggtatgcc ctgcgcaacg cccgctggct gcgggaaaat cccgacaaac atccgatgat
     5101 gtgggcgacg gcgcaggcgc tggtcacgct gccggtttct ctcatcggct atctcgtcgc
     5161 ctgttactgg ctgaatatac aaacgccgga cttctcctta ccttttggcc cccgtccgct
     5221 ggtgtttatt agtctgatgg ttgcgatagc cgtgctttgc tcatgggttg gcgcactctg
     5281 ctggaacgtc gccagccagc gattaccgac agtgattctc gggccgctga ttgttttcga
     5341 aacgctggca ggtttgctgt acaccttttt actccgccag caaatgccgc cgctaatgac
     5401 gctgagcggt atcgcgctgt tagtgattgg cgtggtcatc gcggtcagag caaaaccaga
     5461 aaaaccttta actgaatctg tctcagaaag ttgacatgct ggcagtgagt taaataagcc
     5521 tctgctacgt aagggttata gcttttgcct taaaggtgta tttaaaatac atcttatctt
     5581 attaagaatg aggtatcagc tatggcttat cgcgaccaac ctttaggtga actggcgctc
     5641 tctatccctc gcgcttcggc tctgtttcgt aaatatgata tggattactg ctgtggcggt
     5701 aagcagacgc tggcgcgcgc ggcggcacgt aaagaactgg atgttgaggt cattgaagct
     5761 gaactggcaa agcttgctga acaaccgatt gagaaagact ggcgtagcgc cccgctggca
     5821 gaaatcattg accatatcat cgtgcgctac cacgatcgtc accgcgagca gctgccggag
     5881 ctgatcctgc aagcgactaa agtcgagcgc gttcacgccg acaaaccgag cgtgccaaaa
     5941 gggctgacaa aatacctgac catgctgcat gaagagcttt ccagccacat gatgaaagaa
     6001 gagcagatcc tcttcccgat gatcaaacaa ggcatgggca gccaggcaat ggggccaatc
     6061 agcgtaatgg aaagcgagca cgatgaagcg ggcgaactgc tggaagtgat caaacacact
     6121 accaataacg tcacaccgcc gccagaagcc tgcaccacct ggaaagcgat gtataacggc
     6181 attaatgaac tgattgatga cctgatggat cacatcagtc tggaaaacaa tgtactgttc
     6241 ccacgcgccc tagcgggtga gtgatctaaa agctggatgg cattgcgcca tccagcatga
     6301 taatgcggtt atttccgcag ttcagcagcc cgcttcttac caataaacag ccagcccaac
     6361 cccagcgcga taaaccacag cggggtaacc agcagcgcct ggcgagtgtc atcttccagt
     6421 gtcagcaaca cgaccacgaa cacaaagaac gccatacata cccagcacat cagcttgccg
     6481 agcggcatct tgtagatcga cttttcatgc agatgaggac gctgtttgcg gtacaccagg
     6541 taagagcaaa ggataatcgt ccagacgaac ataaacagaa tcgcggaaac ggttgtaatc
     6601 atcgtgaacg cgccaatcac gctaggattc acatacaaca tcaccacgcc gccgagcaga
     6661 cagatacacg agaaggtcaa ccctttcgcg ggtactgcgc gcttagaaag tttagcgaac
     6721 gctttcggcg caacaccttc ctgcgccaga ccaaacagca tacggctggt agagaagaca
     6781 ccgctgttag cggaagacgc cgcagaggtc agcaccacaa agttgatcac gctggcagcc
     6841 gcaggcaaac ctaccaacac aaacagctca acgaacgggc ttttctccgg gactaccgaa
     6901 ctccacggtg tcacggacat aatcacaatc agcgcgaaga cgtagaacat aatgatacgg
     6961 atcggaatgg agttaatcgc gcgtggcagt gatttctccg gatctttggt ttccgcagct
     7021 gttgtaccta ccagctcaat ccccacgaaa gcgaaaaccg ctatctggaa tccggcaaag
     7081 aagccactta aacctttcgg gaaccagccg ccgtcattcc acaaatgcgc gaatgacgct
     7141 tccacaccgg tcggtgactg aaagtgcatc gccaccatga ccaggccgac gacaatcagc
     7201 gacacgatgg cgacgatttt gatcatcgca aaccagaact ccatctcacc gaacattttc
     7261 acggtggcaa ggttgagcgt cagcagcagc actatcaccg ccagcgaggc gacccagtcg
     7321 gagagatcgg ggaaccagaa ctgagcataa gccgtgatcg ccaccacgtc tgccataccg
     7381 gttacaaccc agcagaacca gtaagtccag ccggtgaaat atcccgccca cggccccagt
     7441 aaatcggaag cgaagtcact aaaagatttg tattccagat tcgaaagcag caattccccc
     7501 attgcccgca tcacgaaaaa gagcataaaa ccaatgatca tataaacgaa aatgatcgac
     7561 ggcccggcaa ggctaatcgt tttgccggac cccataaaca acccagtacc aatggcacct
     7621 ccaatggcaa taagctgaac atgtcggttt gtgagattgc gccgtagcga ctgttcagcc
     7681 ggagcctgat catcggcaac gacttttacc tgatctacca tgttttttct tcctgtacct
     7741 gtctgtgttg ttcaggctct acggccttta gtcagtctat gatacgacga atctgtatcg
     7801 ggactcatcg atattaggta acaatacgcg ggatgaatac taagatttag atctaatgtt
     7861 aattttatgt ttaaagtgag tgtcatatca cggtgataac gcgaaacagc tcacaaaaat
     7921 acacgcttac ggggtctttg caacacaaaa tagcgccgtt tggcaaactg aagggtttat
     7981 tgctgaatgc ctgctcccct ctggttcgag gggaacagaa tgcgctgctt agaggatttc
     8041 cagcagttcg acttcaaaca ccagggtgct gaacggaggg atggatgcac ctgcgccgcg
     8101 ctcgccatat gccagttcct gcgggatagt cagttcccat ttagaaccta ccggcatcag
     8161 agtcagtgct tcaatccagc cagggatcac gccattaacc gggaattcag cgggttcacc
     8221 acgagcaacg gagctgtcaa acacggtgcc gtcgatcagt ttaccggtgt aatgaacacg
     8281 aacgcggtcg gtacgtgccg gaattgcgcc ttcaccctgg ttgatcacgc ggaattgcag
     8341 gccagattcg gtgctattca caccttcttt tttggcgttt tcttccaggt atttcacgcc
     8401 ttcagcagcc atcgcctgga aacgctgacg acgaacggca tcggcgcgtt cgtggatttc
     8461 acgcagcgcg cgatgcacca catcaaccgg cacagccgga tgtttgcctt ccagcgcatc
     8521 ggcaatacct gcaaccagtg cttctggcag cagcccttcc aggccagatt cactcagttg
     8581 ttgccctacc tgcaaaccaa tgccgtagct tgcttgcgct tcgatggtgt caaaagttgg
     8641 ggtggtcatc gtatttcctt tcttagggta taaaagtagc gggcagcata tcagccacac
     8701 ttctttgggt aaaactttgt cgcaggaatg atgacaaatc ccggggaaaa agaaacaata
     8761 gcaatatccc gtatgaatat aataaaggcg ggtttaccac ggacatcata gcgttagctt
     8821 attcataggc tatgattgag gaacaagacg tggagcagga ggaaaccatg cccgggcgct
     8881 ttgaactaaa accaaccctg gagaaagtct ggcacgcgcc ggacaatttt cgcttcatgg
     8941 acccgctgcc gccgatgcat cgtcggggga tcattattgc cgccattgta ttggtggtcg
     9001 gatttctgct tccatctgat gatacgccca acgcgtcggt ggtgacgcgt gaagcgcagt
     9061 tagatattca gtcgcaatca caaccgccga cagaagaaca gccgcaaact acccaaacgc
     9121 aaccattcca gccagatagc ggaattgata atcaatggcg ctcttaccgt gtcgaacccg
     9181 gtaaaacgat ggcgcagctg ttccgcgacc acggactgcc cgctaccgat gtctatgcaa
     9241 tggcgcaggt agaaggcgcg ggtaagccgc tgagtaattt gcaaaatggg cagatggtga
     9301 aaatccgcca gaacgccagc ggcgtggtga cgggcttaac cattgatacg gggaataatc
     9361 aacaggtcct gtttacccgc cagccggatg gcagttttat tcgtgcgcgg tagatgaagg
     9421 gtcaaccaaa agccagctaa ggattgtctc tttgatgacc ttacgtctgg cttccagtgc
     9481 ctggggggaa accatactga tatgataaac taggccaagc gtatgctggt tgatcaaata
     9541 atatccccca agcgcagcaa tattaatatt cacctgcaat gggtcgatac ctggcttaaa
     9601 aatattgtat ttcttccctt catccaataa ggattccatt aactgcaagt gagcatggtt
     9661 aatttctaag agacgctgtg attttgcata atgcacacct ttactttgat tttcactatg
     9721 cacaattttt aaaaaccaag gattagcaat ataataatcc catgtaaaat caataatttt
     9781 ctcaatggct ttttgtggcg gaagatctga gaaatcaagg gccttttctt tttgtcgaat
     9841 atcgttccag gtatattcca gcacttcgat aaataaattt tctttattac caaagtggtg
     9901 ataaaccgtc tgtttactac accctgctgc ctttacaata ttatctactc tggccccttc
     9961 ataaccatat tcggcaaatt cattaaccgc acatagcagc aatttttcct tcaggctctg
    10021 tacgttgctg gtatcaatat acataacgct tccccacgac ctcttcgtgg tgactatcat
    10081 aacctgattt gatttcagct tcagaaaaaa gggcaaaaac ttgtactttc atcaggttat
    10141 caaatgtcct gtgatcgcca tcgcatatca accaactaga tggtttatct ttttatcctc
    10201 cagtcgcaag ctaatcatca ccaaggaaac aaaattcaaa aaagagagct attgtatgaa
    10261 atccactcgt cctgttgccg tgattaccgg agccactcgc ggtattggaa agggctgtgc
    10321 gcaggagctt gctcgtggcg ggtttaacct gctgattaac gatcgtcccg atgccgacag
    10381 cgtagaaaaa ctgcatatca cacaacaaga atgtttggcg gaaggcgtgg aagtgatttg
    10441 cttccctgct gatgtcggtg atctatctct gcatgaggag atgctcgacg cggcgcaaaa
    10501 ccagtggggg cggctggact gtctgctcaa taacgcgggg atttcagtga aaaaacgcgg
    10561 tgacctcctc gacctcgaac cagacagctt cgatcaaaac attgccatta acacccgcgc
    10621 gccgttcttc cttgctcagg cattcagtaa gcgcttactg gcacagccga aaccggaagc
    10681 ggagctgccg caccgctcga ttattttcgt cagttccatc aacgccatca tgctggcgat
    10741 gaatcgcggc gaatacacca ttgccaaaac cgccgtttct gccgctgctc gtctgtttgc
    10801 tgctcgcctt tgtaatgaac aaattggcgt ttatgaagtg cgccctggtc tcatcaaaac
    10861 tgatatgact attccggcaa cggcttatta cgacgaactg attgccaaag gtctggtgcc
    10921 gtggggacgt tggggttatc cggctgatat tgcttccacc gtccgcgcga tggcggaagg
    10981 taaactgatt tacacctgtg gtcaggcggt cgcaatcgac ggcggcttaa gtatgccgcg
    11041 cttctgaggc tgaatcatga gagatctgtt acaacgcccg gatttattca gtatcaatac
    11101 cgcgacactg ggctataaaa cgccgctacc cgccattatt gatgcctgcg ccgcacgcgg
    11161 tattggcgca attgccccct ggcgcaggga gttgcagggt gaagatctcc agcaaatcac
    11221 ccgccagctt gccgccagta acatgagcgt ttccggtttg tgccgtagca cttactacac
    11281 cgcgccgaca ctggcggaac gcaaactggc gattgatgat aaccgccggg cgctggacga
    11341 cgcagcggtg cttaacgccg cctgttatat gcaggtggtt ggcggtctgc caacgggcac
    11401 taaagatcta tatgaagcgc gagaacaagt gaaacaaggg attcgccagt tgctgccaca
    11461 ttcgaaagat gtcggcgtgc caattgcgct ggagccactg cacccgatga cagctgccga
    11521 tcgctcttgc ctgtgcacgt tacgtcaggc gctggactgg tgtgatgaac tcgatcccga
    11581 tggtgagttt ggcctcggcg tggcggtgga tgtttatcac gtctggtggg atccggatct
    11641 cgccagtcag atcctgcgtg ccggaaaacg catcctcgcg tttcatgttt ccgactggtt
    11701 aatgccgacc actgatctgg tcaatgaccg tggtatgccg ggagatggcg tcatcaatat
    11761 tccgtcaatt cgtcggctgg ttgaaaacgc cggatttaat ggtgctattg aactggaaat
    11821 attctcgcct tactggtggc agaaagatat taacagc
//
LOCUS       DAZSNO010000116        11769 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZSNO010000116 DAZSNO010000000
VERSION     DAZSNO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11769
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.116"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..851
                     /locus_tag="ACHWN2_002970"
     CDS             <1..851
                     /locus_tag="ACHWN2_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR8932416.1"
                     /translation="RHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGFN
                     ILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEI
                     VARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEI
                     YESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNP
                     LNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGT
                     IYPIEK"
     gene            873..2282
                     /locus_tag="ACHWN2_002971"
     CDS             873..2282
                     /locus_tag="ACHWN2_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8932417.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="ACHWN2_002972"
     CDS             2260..3039
                     /locus_tag="ACHWN2_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR8932418.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="ACHWN2_002973"
     CDS             3036..3896
                     /locus_tag="ACHWN2_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR8932419.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="ACHWN2_002974"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="ACHWN2_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="ACHWN2_002975"
     CDS             complement(4636..4896)
                     /locus_tag="ACHWN2_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR8932420.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="ACHWN2_002976"
     CDS             complement(4887..6158)
                     /locus_tag="ACHWN2_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR8932421.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHWN2_002977"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACHWN2_002977"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR8932422.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHWN2_002978"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="ACHWN2_002978"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR8932423.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="ACHWN2_002979"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="ACHWN2_002979"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR8932424.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="ACHWN2_002980"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="ACHWN2_002980"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR8932425.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="ACHWN2_002981"
     CDS             11500..11652
                     /locus_tag="ACHWN2_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR8932426.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         2828 a         2991 c         3239 g         2711 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc tgccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacattcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc ctttttttaa atttatattt tttatgtgaa tatatagtct ctatttatta
    11761 ttatgaatt
//
LOCUS       DAZSNO010000117        11682 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZSNO010000117 DAZSNO010000000
VERSION     DAZSNO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.117"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..346
                     /locus_tag="ACHWN2_002982"
     CDS             <1..346
                     /locus_tag="ACHWN2_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR8932427.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
     gene            complement(463..738)
                     /gene="yjfN"
                     /locus_tag="ACHWN2_002983"
     CDS             complement(463..738)
                     /gene="yjfN"
                     /locus_tag="ACHWN2_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR8932428.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="ACHWN2_002984"
     CDS             complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="ACHWN2_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR8932429.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1398..2147
                     /gene="yjfP"
                     /locus_tag="ACHWN2_002985"
     CDS             1398..2147
                     /gene="yjfP"
                     /locus_tag="ACHWN2_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR8932430.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="ACHWN2_002986"
     CDS             complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="ACHWN2_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR8932431.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="ACHWN2_002987"
     CDS             complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="ACHWN2_002987"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR8932432.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4426..5823
                     /gene="ulaA"
                     /locus_tag="ACHWN2_002988"
     CDS             4426..5823
                     /gene="ulaA"
                     /locus_tag="ACHWN2_002988"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFR8932433.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5839..6144
                     /gene="ulaB"
                     /locus_tag="ACHWN2_002989"
     CDS             5839..6144
                     /gene="ulaB"
                     /locus_tag="ACHWN2_002989"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR8932434.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6154..6618
                     /gene="ulaC"
                     /locus_tag="ACHWN2_002990"
     CDS             6154..6618
                     /gene="ulaC"
                     /locus_tag="ACHWN2_002990"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR8932435.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6632..7282
                     /gene="ulaD"
                     /locus_tag="ACHWN2_002991"
     CDS             6632..7282
                     /gene="ulaD"
                     /locus_tag="ACHWN2_002991"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR8932436.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7292..8146
                     /gene="ulaE"
                     /locus_tag="ACHWN2_002992"
     CDS             7292..8146
                     /gene="ulaE"
                     /locus_tag="ACHWN2_002992"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR8932437.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8146..8832
                     /gene="ulaF"
                     /locus_tag="ACHWN2_002993"
     CDS             8146..8832
                     /gene="ulaF"
                     /locus_tag="ACHWN2_002993"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR8932438.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8961..9236)
                     /gene="yjfY"
                     /locus_tag="ACHWN2_002994"
     CDS             complement(8961..9236)
                     /gene="yjfY"
                     /locus_tag="ACHWN2_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR8932439.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9563..9958
                     /gene="rpsF"
                     /locus_tag="ACHWN2_002995"
     CDS             9563..9958
                     /gene="rpsF"
                     /locus_tag="ACHWN2_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR8932440.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9965..10279
                     /gene="priB"
                     /locus_tag="ACHWN2_002996"
     CDS             9965..10279
                     /gene="priB"
                     /locus_tag="ACHWN2_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR8932441.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10284..10511
                     /gene="rpsR"
                     /locus_tag="ACHWN2_002997"
     CDS             10284..10511
                     /gene="rpsR"
                     /locus_tag="ACHWN2_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR8932442.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10553..11002
                     /gene="rplI"
                     /locus_tag="ACHWN2_002998"
     CDS             10553..11002
                     /gene="rplI"
                     /locus_tag="ACHWN2_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR8932443.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11073..11636)
                     /locus_tag="ACHWN2_002999"
                     /pseudo
     CDS             complement(11073..11636)
                     /locus_tag="ACHWN2_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2767 a         2872 c         3087 g         2956 t
ORIGIN      
        1 cggcaatatt atgtgcctgg atgtgttgcg cgttctcaat aagcaagcgg gcgtatacga
       61 cttattgtcg gaagcatttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt
      121 tcgtcgttta cagcagcagc tgcgtaagcc agctgaagaa ctggggcgag agattactca
      181 tcagctattc ctgctgggct gcggtgcgca aatgttgaaa tatgcttctc cgccaatggc
      241 gcaggcgtgg tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat
      301 ccagaatgat ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga
      361 tgcgacgctg gtttcgatta actaaatgaa atatgtgaaa attgtaggcc ggacaaggcg
      421 ctcgcgccgc atccggcatt gttcatttgc tgtagatgac actcatgcat acagtatcgc
      481 ctgtacgcgc cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga
      541 cgcaccttgt tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga
      601 aatattgcta actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc
      661 gaattctgca gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga
      721 ggcaagtaat tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt
      781 ccatcgcggt aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc
      841 atagggacgc aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca
      901 aaatggcttg tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt
      961 aataatcagc ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag
     1021 aaccccgaac catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc
     1081 gaatctcttg cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc
     1141 tacaggcact taacatcaac accaataata aactggcaaa ccggtaaata acgctattac
     1201 gtttcctgct aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa
     1261 ttgttacctg gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa
     1321 cattatgaac taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag
     1381 acaaaaagga gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc
     1441 ttcatgctta tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg
     1501 gctttacttc atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt
     1561 tgcgggtgat catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc
     1621 ggcggttaaa tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac
     1681 gtgcggcaat agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt
     1741 cgatgggcgc gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg
     1801 ccagcatgat gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga
     1861 tacctgaaac ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt
     1921 gggaagcgac aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc
     1981 tcgacgatga cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga
     2041 cgggacggga taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc
     2101 ctgaggcgtt agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc
     2161 tgaccccttg cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca
     2221 gcatatcgat ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc
     2281 tatcaaccag taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg
     2341 tcagcatatc ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc
     2401 agtgcccggc atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt
     2461 tgttgtactg tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg
     2521 ccagcggtag ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca
     2581 gaaacgcggt ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg
     2641 ccgctttagc aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg
     2701 tccagcgcgg gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc
     2761 cgctttcgcc aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct
     2821 caacgacttt ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat
     2881 gtctttgtgc ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg
     2941 aaatagccag gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac
     3001 gacagattac aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa
     3061 accgcgcgga tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc
     3121 aacctgccag ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag
     3181 cacgcggatc tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat
     3241 aaccactttc gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc
     3301 ggtaataccg cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt
     3361 accgtgcttc gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc
     3421 aggggttttg aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc
     3481 cgctttttga tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg
     3541 aatttcaatg tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt
     3601 cggtacgccc cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc
     3661 atcagcacag ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc
     3721 gtgagtcgcc agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt
     3781 ggtgcgtagg tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg
     3841 tttcattaac gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac
     3901 gttggtgcca ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa
     3961 tgtgccaggg gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg
     4021 gaaagtgctc aggatccagg attctcgggt gatacttttc actttactca tcgccattta
     4081 ccttcatgat agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat
     4141 tccttcagga aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt
     4201 gagtggggaa agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata
     4261 attgtttcgt tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca
     4321 tttaatcata aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt
     4381 ccgggtgtgc ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac
     4441 aacatcttta ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt
     4501 gtgacctgtc tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg
     4561 attaaaacca taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc
     4621 ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat
     4681 acttacgcgt cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt
     4741 tacgcggtgt tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc
     4801 ggtattcgta ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc
     4861 gttacgttgt ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg
     4921 gtttcgctct actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg
     4981 gacggctgcg gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa
     5041 gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg
     5101 ctgaatattt tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt
     5161 gccattctgc tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg
     5221 acggtctata tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag
     5281 ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg
     5341 atccctggtg cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc
     5401 gtggtctggg gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc
     5461 gcctgcggtt cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc
     5521 accatcggcg tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg
     5581 gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt
     5641 gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc
     5701 tccatcggta tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc
     5761 gctggccgcg cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct
     5821 taataaggag ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc
     5881 agttccatga tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac
     5941 acggtaaata gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc
     6001 atcgcttcta cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc
     6061 gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg
     6121 catttcccgc aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag
     6181 aataaatcca tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc
     6241 gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     6301 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     6361 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     6421 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     6481 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     6541 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     6601 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     6661 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     6721 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     6781 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     6841 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     6901 ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca
     6961 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     7021 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     7081 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     7141 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     7201 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     7261 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     7321 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     7381 gttgggcttc gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct
     7441 cgactggagc cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg
     7501 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     7561 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     7621 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     7681 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     7741 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     7801 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     7861 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     7921 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     7981 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     8041 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgtgtgattg
     8101 ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa
     8161 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg
     8221 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     8281 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     8341 gaaggggagt atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac
     8401 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg
     8461 gggctggcga tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg
     8521 tgtacgcgtg gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa
     8581 gtgattatcg aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat
     8641 cagcacggac cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg
     8701 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat
     8761 atcgacagct acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac
     8821 gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt
     8881 ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
     8941 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
     9001 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct
     9061 tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg
     9121 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
     9181 catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat
     9241 acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta
     9301 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt
     9361 gacggaatgt gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt
     9421 atttctgctc cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca
     9481 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
     9541 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
     9601 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
     9661 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
     9721 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
     9781 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
     9841 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
     9901 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
     9961 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
    10021 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
    10081 agccggcttt caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa
    10141 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
    10201 atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
    10261 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
    10321 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
    10381 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
    10441 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
    10501 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
    10561 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
    10621 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    10681 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    10741 agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    10801 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    10861 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    10921 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    10981 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg
    11041 tcggcgtttt gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca
    11101 ttctcttgtg tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt
    11161 atgtctcggt cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt
    11221 gataaggaac gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat
    11281 gcagcaatga tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata
    11341 taaagatcga ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt
    11401 acaacgtatc ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca
    11461 acctcgtgta ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat
    11521 ttcagttctg cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaata
    11581 ttatctaatg tagcttcatg gttccatcca aaagaattat caccattcat tgttactgaa
    11641 tcgccacggg tttaacagac acctcagagt catttaagat gg
//
LOCUS       DAZSNO010000118        11400 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZSNO010000118 DAZSNO010000000
VERSION     DAZSNO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11400
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.118"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(95..691)
                     /gene="nrfG"
                     /locus_tag="ACHWN2_003000"
     CDS             complement(95..691)
                     /gene="nrfG"
                     /locus_tag="ACHWN2_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR8932444.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(688..1071)
                     /gene="nrfF"
                     /locus_tag="ACHWN2_003001"
     CDS             complement(688..1071)
                     /gene="nrfF"
                     /locus_tag="ACHWN2_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR8932445.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(1064..2782)
                     /locus_tag="ACHWN2_003002"
     CDS             complement(1064..2782)
                     /locus_tag="ACHWN2_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR8932446.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(2802..3758)
                     /gene="nrfD"
                     /locus_tag="ACHWN2_003003"
     CDS             complement(2802..3758)
                     /gene="nrfD"
                     /locus_tag="ACHWN2_003003"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR8932447.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(3755..4426)
                     /gene="nrfC"
                     /locus_tag="ACHWN2_003004"
     CDS             complement(3755..4426)
                     /gene="nrfC"
                     /locus_tag="ACHWN2_003004"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR8932448.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(4423..4989)
                     /gene="nrfB"
                     /locus_tag="ACHWN2_003005"
     CDS             complement(4423..4989)
                     /gene="nrfB"
                     /locus_tag="ACHWN2_003005"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR8932449.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(5034..6470)
                     /gene="nrfA"
                     /locus_tag="ACHWN2_003006"
     CDS             complement(5034..6470)
                     /gene="nrfA"
                     /locus_tag="ACHWN2_003006"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR8932450.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6863..8821
                     /gene="acs"
                     /locus_tag="ACHWN2_003007"
                     /pseudo
     CDS             6863..8821
                     /gene="acs"
                     /locus_tag="ACHWN2_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            9021..9335
                     /locus_tag="ACHWN2_003008"
     CDS             9021..9335
                     /locus_tag="ACHWN2_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFR8932451.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            9332..10981
                     /gene="actP"
                     /locus_tag="ACHWN2_003009"
     CDS             9332..10981
                     /gene="actP"
                     /locus_tag="ACHWN2_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR8932452.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            11159..>11400
                     /locus_tag="ACHWN2_003010"
     CDS             11159..>11400
                     /locus_tag="ACHWN2_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR8932453.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKIT"
BASE COUNT         2708 a         3162 c         2958 g         2572 t
ORIGIN      
        1 atctgcaata atactgattg gtgattattt ttggcatatg ttcattcgat tattttttat
       61 gcagctcaat gacataatct tcatcaccag atgattaatc cgatctccgc tgcaacagtt
      121 ttgccatatt aatcgactcg accagctgtg tccggttgat ccggggtgag ttgagatcca
      181 tcactttttg ccatagttcg atggcttgcg cgtaattcgc ctgcataaac gcatcggaag
      241 ccagcagcat cagagcggtg atttcgttag agtccagcgc gagggctttg tcgatcattg
      301 cgcgagtctg ggcggtcata tgctggctgg cctggtaata aagcaccgtc gccagcgccg
      361 catacagttc agcgttctca ccacgtagtt gcaacgcctg acgatacgcc agcagcgaat
      421 tgctgtaatc gttttgccac agatagtact cgcccagtaa cgcccattgt tcgctgtttt
      481 gtggattagc gcggatttta tcctgcaatg cctgaagctg cgcttccggg gtttgctggc
      541 tggcaaactg atgtagcgga tcgcgctgac gctgatactc cgcacgtacc gcctgccatt
      601 ttgggcttaa cagataactg ccgacacaga ggaataccat caatatcgtg agtgttgtca
      661 gcattttcac cggtatttga ggctgtttca tcgcttagcc ctcactcgcc agaggatcag
      721 tgccatcagc aataacaaca ccactggcag cgcccacaac accagcgtct gacccgttaa
      781 cggtgggtta tagcgaacaa aatctccgta gcgttcggtc atccagccaa tgatttcgac
      841 ttcgttcttc ccctccgaca ccatgctata aacctgatgg cgcatactga ccgccaccgg
      901 cgcattggat tcgagtaagt tttgattctg gcactgcgga caacgtaact ggctggcaat
      961 atttaacgcc tgttgctgtt gttgcggatt ggcgaattgc caggtgtcta caacctgagc
     1021 gcgcgcaaaa caggtaaata acagcagtag cgtgagaagc cctttattca tcgcgcttct
     1081 tcccccgcca tccgcttaac aatgcgcccg caatcatcaa caatcctccc ccccagatcc
     1141 agcgcacacc gctttgtaca tacaaacgaa aagcgtaacg atccgctctg gttttttcac
     1201 ccatgaccgc ataccagtca tggatgccgt tccagcgaat tgacggttcc atcatttgct
     1261 gacggcgtgc ttcataaaaa cgccgctccg gcgttaattc accaatgcgt tgctgatggt
     1321 caaacagcgc cactatcgct ttttcgctgg tgtaattgcc tctggcttgc agatcgagtc
     1381 gctcaaaacg gaaggtgtat cctgccagcg tcacctgctg acccggctgt aaattcaggc
     1441 tgatctcctg acgactgaca ctggaaacca cgatccccgc ggcaaataac agcacgccag
     1501 catgagctac cagcgccgga agctgcacgc gtttgccaga gacaaacgtc gccagcacaa
     1561 tcaccaccag catcaacaga ccaaacggta acgtcgcgcg gttaaaatac ggcgcgccaa
     1621 cggagaggcg tccccagccc agcaggccgt aaatcatcgg ataaagcgtt cccaccagca
     1681 cgatcagtag cactgcgcaa aacagcaaca gcgtagcgag gattaacatt tcccgcgata
     1741 accccgaaaa acgcaccgcc gggccaccgt cccgcgctcg ccagccatac agagccaaag
     1801 acgcaaggct aatcagtgca aacaggctga acaacggcac ggcgcggacg ttatccagtg
     1861 cgaacgcatg aaccgaaacc agaatgccag aacggacaat taaggtgccc agcagcgaca
     1921 gcatcagagt aactatcgcc agcaacagcg accagtggcg gaaaatcccc cgctggcgtg
     1981 tcagggacaa actgtgcagc agcgcagtgg cagaaagcca gggtaataaa gaggcgtttt
     2041 ccaccggatc ccagaaccac cagccgcccc agccgagttc gcagtaggcc caccaggaac
     2101 cgaggatgat ccccgccgtt aatgcactcc agccaggtaa cgcccagcgc cagcaaattc
     2161 gggcgctggc cgcatcaaac tcgccgcgca gtagactcgc cagcgccacg ctcgccgcta
     2221 ccatcaaacc gccatagcca aggtaaagca gcggtggatg aaagataaga ccgggatgtt
     2281 gcagcatcgg attgagatcg cggccttcga ttgctggtgg aaatatccgc acgaagggat
     2341 cggaccacag caccacaaac agtagcagtg cggcgagcat gagagataaa acggctagcg
     2401 tcagcggaaa tagcggatcg ctttgctgcc gataatgcca ggcaaacagc gcgctccagg
     2461 cggaaagcag cagcacccaa agcagcagcg aaccttcatg accgccccac accgccgcca
     2521 gtttgagttc ccacgacagc aggctgtagc tatgttgggc gacataaatg accgagaaat
     2581 cgctgatgag aaaacaatac gtcagtacgc caaaggcgag cagcaggagc gcgaactgcg
     2641 ccagaatgcc gatgcaagtg agtcgcatca tggcaggcca gcgcaaccgc actcccgcaa
     2701 aggccgtcaa cggggtcaac acgttgaccc cgagacttaa caacaacgcc agaaaaccga
     2761 cttcaggaag gaatacgtcc agaaaccacc tcttttctgg cttacgccac cgtcaactgt
     2821 ccggcgtaga gaatgaaaaa gcgcagcatc aacacgccga ccagactggc cccacacgcc
     2881 gccagcacgg caggaatgcc ggaactgcga ttgacccacg gtttgagcaa cattggcaca
     2941 atcagcccca gcccggcgac accaagccag aaccaccacg tccagaaacc gccacctaat
     3001 gccgccacca gcgcacgaac tttaccgtca tcgcccagcg ccagaccgac aaaaaacgcc
     3061 accagcagga agatttcacc ccataccacg gggatttcca tccggtgaac aaagtgcgct
     3121 tccgtggaat gcgggttact gcgttggcgt atcgccatgg cgatcagcgc caccgccgca
     3181 ccggacgaga tgccggagaa gaggagcagc accggcaggc tcgggttatt gaggaacgga
     3241 tacgatttca gcgccgacag cagaaagccg gtataagccc ccagcagcac cgccaacacc
     3301 agcatcagcg tttccagtcc gcgatgaacg ggcgtcagca ggctcagaac cttttgcacg
     3361 atccccagct tcggcaacca gcgttgttgc agggcaagca aatcatgttc aaagattttc
     3421 gccagccaca gcaccagcac caccatgtag agctgaaaca gcatcacccc catcgacatc
     3481 accgaggtaa aactgtagtg gaacatcagc ttccagaagg tccacggtct tgtcaggtgg
     3541 aagacgagga tcaacagacc gaggatcacc gcgcccggcc cgacaatcag cgtggtgcgc
     3601 agcaacgtac tgtctgcacc gcccgcctgc gggtagaagc gacgtaacag cacggccagc
     3661 gtcaccagac cggcagaaat accaatcaaa aacaggtaga tggcaatcgg ccagtcccac
     3721 accagcgatt caaaatgaaa tgcggaagtc tgcgtcattg gctcacctcc ccgtatttaa
     3781 acggtacgcg gtacagcttc ggtttggttc ccagcgccag cttgtagcgg taagtaggct
     3841 tctggcgcag cagttgcgaa atctcactgt taggatcgtc cagattgcca aacgtcagcg
     3901 ccttcgtcgg gcaagcttca acgcacgcgg gaagcttacc ggcctgcaaa ttggtcttac
     3961 gacagaaatc gcatttgtcc gccgtcttcg tgaccggatg gataaagcgc acgcggtacg
     4021 gacaggcggc gatgcagtac tgacaaccga cgcagagatc aggattaaca tcgacaatcc
     4081 cgctagcggc atcacgaaaa gacgcaccgg tcgggcagac gtcaacgcac ggcgcatgat
     4141 cgcagtgctg gcaagactta cggaagaagc gatatttcac atcaggaaat tcgccctgcg
     4201 gctcgctacg aataatcgtc aggcgcgaga cgccttccgg caccttgttc acttcccgac
     4261 aagcatccat acaggcggta cagccgatgc ataacgactc gtcatgcacc atgccgtaac
     4321 gcaccccatt gatattcaac gttttcgcca ccacgcgccc tgcggtgccg ctgacggctg
     4381 ccagcacgcc gacgccggtg agaaactggc gacgagacca ggtcatggct gctccttaag
     4441 caacggaaca gacgccgggt taaagttcgg attggtgcgc tgatcgctgt ggcaatcgac
     4501 gcaaatctta atccgtcctt tgtcacttaa cgtctgcatc gtatcttgct gtggatgcag
     4561 ggaatggcag ctggcgcacg ccactttggt tacgtggaca tcgtgcggcc agaacgcttt
     4621 ttgcaactgt tccggcagat gacaggacat acagacgctg ttctgctctc cgaccttata
     4681 catcggctcg ttaaagcgca tcacatcttt caccccttcg cggtgttgcg gtgatgcttg
     4741 gccgtgacag ttggtgcagg tgaccggcaa tttgttattc gggttgataa cggaagcatg
     4801 tttgccatgc ataccttcgg tatctggttt atgacagtcc agacaggcgg catccgggtt
     4861 acgctgctgg gtaacttcgt agcgatcacc agatgcctgc accgcagggg tagcggtaat
     4921 tccgttcagg ctccacaaca ggcccgacgc cagcacccca gcagttaata acgaacgtaa
     4981 tacgctcata ttcactccat tgtgttttcc gccccttgcg aggcggaacg gggttattgg
     5041 cttaacagac cgtttttacg tgcctgctct tcccactgcg ggatcaccgt tttgatgaag
     5101 tcctgcttct cggccttgat ttgttccatg ttcaggccaa tggcctgctg ggctttctct
     5161 ttggtcgaga tatccgggat ctggatttca tgggtgatac ctttggtcgc cagcaggcgc
     5221 gccagtttgg tgcgcgcatc cgccgcttta tccatcgccg taccgagcat ccgtagacct
     5281 tcttccggtg cgtgcatatg aataccgtgg gaagcgatcg ccagatccca acgccactgg
     5341 gcatgacgga tatcgtcctg aatcggcttc atttcggctt ccgtcgcgcc tgcatccagc
     5401 gccgctttcg cttcgaagtg agcgtgaacc agttgatctt caaccttgat tttcaggtcg
     5461 ttaatcgatt gcttacgttc cgcgaccact ttttgcaagg cagctttatc ctgggtatgg
     5521 cagttcgcac aagtctgggc gaagttatca aacggattac caattttatg gtcggtgtag
     5581 agtttgcctt cggcgttctg cacttttggc atatggcagt cgatacaggt cacgttgttt
     5641 ttaccgtgaa tgcccgctgt ccaggtttca tattccgggt gctgcgcttt cagcattggc
     5701 gttttcgaca gggagttagt ccagtcagag aaggcaattt tgtcgtaata ctgctccata
     5761 ttttcgactt tcatgccgtc atcccacggg aatttaaccg ctttgttttt gccgtcgaag
     5821 taatactcca catggcactg accgcaaacc atcgattgct ggtcgaaacg tccggctttc
     5881 tcaaaaggtt taccaatggc ttccatcgcg cgagccgcat acggacggga aagggttaac
     5941 tccggtttgc ctttggcgaa ctctggtgag gcggtgttat ggcagtcggc acaacctaag
     6001 ttgttgacga tttccgggcc gccgcgcgcc catttaccgt ggaagtagcc atcttcgccg
     6061 tctttctgga tcagacgcgc cacatccggg cttttacaac tccagcacgc catcggtagc
     6121 ggaccatctt cagcgttttt cggcgcaccg gtacgcaggg tttcacgcac atcggtcaca
     6181 gcaaaggcat gtccacgagg cttgttgtaa tcgcgcgaga agggataccc cgcccacagg
     6241 atcaccagcc gtggatcttc cgccagggcg tcaacacgct ctgactgttc cgaggtggct
     6301 ttccaggaga gatattgatc gtgatgctgc ggggcaaagg tttcattctt cgcttccaca
     6361 gttacaggtt ttgcgggagc tgccgtttgt tcagcgtgaa cagaagtgaa aaagaaaaaa
     6421 ggaatcaata agctgaagat acggcgtgcg tttattttta tccttgtcat aggggcttca
     6481 tccgaattgc gccattgttg caatggcggt ttttattgtt tttcacgaca gtaaccgcac
     6541 ctacactgtc atgacattgc tcacccctat gtgtaacaaa taaccacact gtgaatgttg
     6601 tctttaatca attgtaagtg catgtaaaat accactttag agttagtcag tatcttcctc
     6661 tttttcaaca gcatgcataa ctgcatgttc ctcaaagaat taatcaactt ttgttggtga
     6721 ccttcaaaaa ttaccctgcc gtttatttgc accattctac ttttgcgtga tctgtcgccc
     6781 aaatactaaa caaaactgcc aataccccta catttaacgc ttatgccaca tattattaac
     6841 atcctacaag gagaacaaaa gcatgagcca aattcacaaa cacaccattc ctgccaacat
     6901 cgcagaccgt tgcctgataa accctcagca gtacgaggcg atgtatcaac aatctattaa
     6961 cgtacctgat accttctggg gcgaacaggg aaaaattctc gactggatca aaccgtacca
     7021 gaaggtgaaa aatacctcct ttgcccccgg taatgtgtcc attaaatggt acgaggacgg
     7081 cacgctgaat ctggcggcaa actgccttga ccgccatctg caagaaaacg gcgatcgcac
     7141 cgccatcatc tgggaaggcg acgacgccag ccagagcaaa catatcagct ataaagagct
     7201 gcaccgcgac gtctgccgct tcgccaatac cctgctcgag ctgggcatta aaaaaggtga
     7261 tgtggtggcg atttatatgc cgatggtgcc ggaagccgcg gttgcgatgc tggcctgcgc
     7321 ccgcattggc gcgatgcatt cggtgatttt cggcggcttc tcgccggaag ccgttgccgg
     7381 gcgcattatt gattccaact cacgactggt gatcacttcc gacgaaggcg tgcgcgccgg
     7441 gcgtagtatt ccgctgaaga aaaacgttga tgacgcacta aaaaacccga acgtcaccag
     7501 cgtagagcat gtggtggtac tgaagcgtac tggcggaaac atcgactggc aggaagggcg
     7561 cgacctgtgg tggcacgacc tggttgagca agccagcgat cagcactagg cggaagagat
     7621 gaacgccgaa gatccgctgt ttattctcta tacctccggt tctaccggaa aaccaaaagg
     7681 cgtactgcac actaccggcg gttatctggt gtacgcggcg ctgaccttta aatatgtctt
     7741 tgattatcat ccgggtgata tctactggtg caccgccgat gtgggctggg ttaccggaca
     7801 cagttatttg ctgtacggcc cgctggcctg cggcgcaacc acgctgatgt ttgaaggcgt
     7861 accgaactgg ccgacgcctg cccgtatggc gcaggtggtg gacaagcatc aggtcaatat
     7921 tctctatacc gcacccacgg cgatccgcgc gctgatggcg gaaggcgata aagcgattga
     7981 aggcaccgac cgttcgtcgc tacgcattct cggttccgtg ggcgagccaa ttaacccgga
     8041 agcgtgggag tggtactgga aaaaaatcgg caacgagaaa tgtccggtgg tcgatacctg
     8101 gtggcagacc gaaaccggcg gtttcatgat caccccgctg cctggcgcta ccgagctgaa
     8161 agccggttcg gcaacacgtc cgttcttcgg cgtgcaaccg gcgctggtcg ataacgaagg
     8221 taacccgctg gaaggggcta ccgaaggtag cctggtgatc accgactcct ggcctggtca
     8281 ggcgcgtacg ctgtttggcg atcacgaacg ttttgagcag acctattttt ccaccttcaa
     8341 aaatatgtat ttcagcggcg acggcgcgcg tcgcgatgaa gatggctatt actggatcac
     8401 cgggcgtgtg gacgacgtgc tgaacgtctc cggtcaccgt ctggggacgg cagagataga
     8461 gtcggcgctg gtggcgcatc cgaagattgc cgaagccgcc gtagtaggta ttccgcacaa
     8521 tattaaaggt caggcgatct acgcctatgt cacgcttaat cacggggagg aaccgtcacc
     8581 agaactgtac gcagaagtcc gcaactgggt gcgtaaagag attggcccgc tggcgacgcc
     8641 agacgtgctg cactggaccg actccctgcc taaaacccgc tccggcaaaa ttatgcgccg
     8701 tattctgcgc aaaattgcgg cgggcgatac cagcaacctg ggcgatacct cgacgcttgc
     8761 cgatcctggc gtggtcgaga agctgcttga agagaagcag gctatcgcga tgccatcgta
     8821 acccacaatt gccggatgcg aatcggtaac ggtttgtagg cctgataaga cgcgacagcg
     8881 tcgcatcagg cattgattgc cggatgcggc gtaaacgcct tatccggcct acattcggca
     8941 agggttaccc gagcgttaac cttctcccat aagggagcgg gaattaaaac aatccctaca
     9001 ttacctctgg agaatctgtg atgaatggta cgatttatca gcggatagaa gacaatgcgc
     9061 atttcaggga gttagtcgaa aaacggcaac ggtttgccac catcctgtcg attattatgc
     9121 tggcagttta tatcggcttt attttactga tcgccttcgc gcccggctgg ctgggcacgc
     9181 cgctgaatcc gaacaccagc gtcacacgcg gtattccaat tggtgttgga gtgattgtga
     9241 tctcctttgt tctcaccggt atctacatct ggcgggcgaa cggcgaattc gaccgtctta
     9301 ataatgaagt cctgcatgag gtacaagcat catgaaaaga gttctgacgg cgcttgccgc
     9361 cacactccct ttcgcagcta acgccgcgga tgctattagc ggggccgtag agcgccagcc
     9421 aacgaactgg caggcgatta ttatgttcct gattttcgtc gtgtttacgc tcggcattac
     9481 ctactgggca tcaaaacgcg tacgttctcg tagcgactac tacaccgcag gcggcaatat
     9541 cactggcttc cagaacgggc tggcgattgc cggggactat atgtccgccg cctcattctt
     9601 ggggatctcc gcgctggtgt ttacctccgg ctatgacggg ctgatctact cgttgggctt
     9661 cctggtgggc tggccaatca ttctgtttct gattgccgaa cgtctgcgta acctgggacg
     9721 ctacacctct gccgatgtgg cctcttatcg tctgaaacaa gggccgattc gtattctttc
     9781 ggcctgtggt tctctggtag tggtggcgct ttaccttatc gctcagatgg taggcgcagg
     9841 taaactgatc gagctgctgt ttggccttaa ctatcacatt gcggtggtgc tggtcggcgt
     9901 gctgatgatg atgtacgtcc tgttcggcgg catgctggcg accacctggg tacaaattat
     9961 caaagctgtg ctgttgctgt tcggtgccag ctttatggcc tttatggtga tgaaacacgt
    10021 cggctttagc ttcaacaatc tgttcagcga agcgatggcg gtacacccga aaggtgtcga
    10081 catcatgaaa ccgggcggac tggtgaaaga tccgatctcc gcgctctctc tggggctggg
    10141 actgatgttt ggtacggcgg gcttgccgca cattctgatg cgcttcttta cagtcagcga
    10201 tgcccgcgaa gcacgtaaga gcgtgttcta cgccaccgga tttatgggct acttctatat
    10261 tctgaccttt attatcggct tcggcgcgat catgcttgtt ggtgcgaatc cggaatataa
    10321 agacgcggcg ggccatctga ttggtggtaa caacatggcg gccgttcacc tggcgaatgc
    10381 agtgggcggc aacctattcc tcggttttat ttcagcggtt gctttcgcca ctatcctcgc
    10441 ggtggttgcg gatctgacgc tggcgggcgc atcggcggtt tcgcatgact tgtacgctaa
    10501 cgtcttcaaa aaaggcgcga ccgaacgtga agagctgcgg gtatcaaaaa tcaccgtact
    10561 gatcctcggc gtgattgcga ttatcctcgg cgtgctgttt gagaatcaga acatcgcctt
    10621 tatggtaggc cttgcgtttg ccatcgcggc gagctgtaac ttcccgatca ttctgctttc
    10681 catgtactgg tcgaaactga ctacccgtgg cgcgatgctg ggcggctggc tggggctgat
    10741 taccgcagtg gtgctgatga tcctcggccc gactatttgg gtacagatcc tcggtcacga
    10801 aaaagccatc ttcccgtatg aatacccggc gctgttctct atcagcgtgg cattcctcgg
    10861 catctggttc ttctcggcaa ccgataactc agcggaaggg gcgcgcgagc gtgaactgtt
    10921 ccgcgcgcag tttatccgct cccagaccgg ctttggcgtt gagcaaggcc gcgcacatta
    10981 atctctcccc ttccccggtc gcctgaccgg ggaatacgct tcctctccaa catgcatcac
    11041 cttttcccat aatattaaac aaataaactc attaaaaaat gagcgatttt tgacagtcgt
    11101 agaaaatgat aatgcagaga atatgccttt tctttcttgt taattataag gatattttat
    11161 gcgctataat ggtttaaata atatgttttt ccctctttgc cagattaacg ataaccactc
    11221 tatcacaagt tcatcacata caaagaaaac aaaatcagat aattacagca aacatcataa
    11281 aaacacgtta attgacaata aagccctctc tcttttcaaa atagatgatc atgaaaaagt
    11341 gataggcttg attcagaaaa tgaaaagaat ttatgatagt ttaccatcag gaaaaatcac
//
LOCUS       DAZSNO010000119        11348 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZSNO010000119 DAZSNO010000000
VERSION     DAZSNO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11348
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.119"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="ACHWN2_003011"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="ACHWN2_003011"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR8932454.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /gene="malI"
                     /locus_tag="ACHWN2_003012"
     CDS             complement(721..1749)
                     /gene="malI"
                     /locus_tag="ACHWN2_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR8932455.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /gene="malX"
                     /locus_tag="ACHWN2_003013"
     CDS             1924..3516
                     /gene="malX"
                     /locus_tag="ACHWN2_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR8932456.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="ACHWN2_003014"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="ACHWN2_003014"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR8932457.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4802..5803
                     /gene="add"
                     /locus_tag="ACHWN2_003015"
     CDS             4802..5803
                     /gene="add"
                     /locus_tag="ACHWN2_003015"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR8932458.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5839..6879)
                     /locus_tag="ACHWN2_003016"
     CDS             complement(5839..6879)
                     /locus_tag="ACHWN2_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR8932459.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7122..7247
                     /gene="blr"
                     /locus_tag="ACHWN2_003017"
     CDS             7122..7247
                     /gene="blr"
                     /locus_tag="ACHWN2_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR8932460.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7520..7735
                     /gene="ydgT"
                     /locus_tag="ACHWN2_003018"
     CDS             7520..7735
                     /gene="ydgT"
                     /locus_tag="ACHWN2_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR8932461.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7821..8261
                     /locus_tag="ACHWN2_003019"
     CDS             7821..8261
                     /locus_tag="ACHWN2_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR8932462.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8338..8919
                     /gene="rsxA"
                     /locus_tag="ACHWN2_003020"
     CDS             8338..8919
                     /gene="rsxA"
                     /locus_tag="ACHWN2_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR8932463.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8919..9497
                     /gene="rsxB"
                     /locus_tag="ACHWN2_003021"
     CDS             8919..9497
                     /gene="rsxB"
                     /locus_tag="ACHWN2_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR8932464.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9490..>11348
                     /gene="rsxC"
                     /locus_tag="ACHWN2_003022"
     CDS             9490..>11348
                     /gene="rsxC"
                     /locus_tag="ACHWN2_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR8932465.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPR"
BASE COUNT         2650 a         2926 c         2864 g         2908 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgctattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccacctc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagattact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccatccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgtgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccattaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctac caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga catcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc aatcgtggat
     4741 ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa agagtgcgac
     4801 catgattgat accaccctgc cattaactga tatccatcgc caccttgatg gcaacattcg
     4861 tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg cacaatccct
     4921 tgaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg tgagctttct
     4981 gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc
     5041 atttgaaaac attgaagatg cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc
     5101 accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg aagcggtgat
     5161 cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta tcggcattat
     5221 gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt tagcccaccg
     5281 tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg gaagtctgtt
     5341 cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc atgcaggcga
     5401 agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg
     5461 acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg agcaacaaat
     5521 tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag agctggctgc
     5581 acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc
     5641 cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag ctgctgggtt
     5701 atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt tcctcagcgc
     5761 agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca
     5821 aattgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg cggattcgat
     5881 gcccagctca atcaactcca ttacctggat tgcctggctt gccggaaccg gattttcacc
     5941 atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc caggcaccgt
     6001 caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc catcacgcat
     6061 atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg
     6121 atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc gtgctgactc
     6181 agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg acaagatggc
     6241 gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct
     6301 gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta aatcgtacca
     6361 gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg
     6421 gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga gcaaaccttt
     6481 tagcgtcaag aaatcgctat cccaacggcg gttatggaat acagacagca cacgccccag
     6541 gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc
     6601 aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat gggtatcgtt
     6661 gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct tcggctcaga
     6721 gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc tggagattac
     6781 cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt tgctcgcata
     6841 accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc tcattgaagt
     6901 atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac ctgccagcaa
     6961 taagggatgt tgtttaactt aagtcaaaaa aatagcgaat tttccaacga caaaagctaa
     7021 atatcgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt aacatttacc
     7081 ccctgaagtt aatggatcaa tcaagagaga tgtgggctgt aatgaatcgt cttattgaat
     7141 taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg agtcacattg
     7201 acaactatca gccacctgaa cagagtgctt cggtacaaca caagtaagct ctgcacttgt
     7261 ggagcgacat gctgcccgtc cgggtgcatg ttttcacttg tcggatatta aaccaggaat
     7321 ttattatctt gttcgatgtt gttggtgatt gtcagggata gtaaagcgcg ttactctttt
     7381 cagagcagtt tttcatccct gaatatttgc catcagcgca ttcttattat tgtaataaca
     7441 caagcgttca cttttggtga aattttatca tcactaataa tacctacccc tttggtatta
     7501 attattttat ggacctttta tgactgttca ggactactta ttaaaatttc gcaaaatcag
     7561 ttcactcgaa agtctggaaa aactctacga ccatcttaat tacaccctga cggacgatca
     7621 ggaactgatc aatatgtatc gtgctgccga tcaccgtcgc gcagagctgg tttctggcgg
     7681 gcgtttgttt gacctcggcc aggtaccgaa gtccgtctgg cactatgtcc aataaagaaa
     7741 gtagcgatcg gttataaaag ccttataata acgcccctaa aattagattt gcctgtttgc
     7801 gtcgctattc tggagaacct atgaccacca caacgccaca acgtattgga ggctggttgc
     7861 ttggcccgtt ggcctggctt ttagtcgctc tgttgagtac gacgctggcg ttgttgctgt
     7921 ataccgctgc gttatcttct ccgcaaacat ttcaaacgct cggcggacaa gcactgacga
     7981 cacaaatctt atggggcgtt tcttttatta ccgctatcgc catgtggtat tacacactgt
     8041 ggttgaccat cgctttcttt aaacgtcggc gttgtgttcc taaacactat attatctggc
     8101 tgcttatttc cgtattgctg gcagtaaaag cattcgcttt ttcacccgtg gaagttggca
     8161 tagccgttcg ccagttgctg tttactttgt tggcaaccgc attgatcgtc ccctatttca
     8221 aacgctcgtc gcgggtaaaa gcgacgtttg tgaatccgta ataaccttac agttaacctg
     8281 ttgtcgcctg ctctggatta acggataata ggcggctttt ttatttcagg ccgaaaaatg
     8341 actgactacc tgttactgtt tgtcggaact gtactggtca ataactttgt actggtcaag
     8401 tttctcggtc tctgtccgtt tatgggggtt tccaaaaaac tggaaaccgc gatgggcatg
     8461 gggctggcaa caacgtttgt gatgacgctg acgtctattt gcgcctggct tatcgatacg
     8521 tggattttga tcccacttaa tctgatttac ctgcgcaccc tggcatttat tctggtgatt
     8581 gctgtggtcg tgcagttcac cgagatggtg gtgcgcaaaa ccagcccggt gctttaccgt
     8641 ctgctgggga tttttttgcc gcttatcacc accaactgtg cagtgctcgg cgtggcgttg
     8701 ctgaatatca atctcgggca caatttcttg cagtcggcgc tgtacggttt ttccgccgct
     8761 gtcggtttct cgctggtgat ggtgctcttc gccgccatcc gcgaacgcct tgctgtggct
     8821 gatgtcccgg caccttttcg cggtaatgcc attgcgttaa ttaccgcagg tcttatgtct
     8881 ctggccttta tgggctttag tggtttggtg aagttgtaat gaatgctatc tggattgccg
     8941 ttgccgccgt gagcctgctg ggcctggcgt ttggcgccat tctgggttat gcctcccgcc
     9001 gttttgcggt ggaagacgat ccggtcgttg agaaaattga cgaaatctta ccgcagagcc
     9061 agtgtggtca gtgcggttat cccggctgtc gcccctacgc ggaagccatc agctgtaacg
     9121 gtgaaaaaat caaccgttgc gccccaggtg gcgaagctgt gatgctaaaa attgccgagt
     9181 tgcttaatgt cgagccgcag ccgctggatg gcgaagcgca agagctaacg cctgcgcgga
     9241 tggtggcggt tattgatgaa aataactgta ttggctgcac taaatgtatt caggcttgtc
     9301 cggtagacgc catcgttggc gctacccgtg tcatgcatac ggtaatgagt gatctctgta
     9361 cgggctgcaa tttatgtgtt gatccgtgcc cgacgcactg catctcgttg caaccggtcg
     9421 cagaaacacc tgactcctgg aaatgggatc tgaacaccat tcccgtgcgt atcattcccg
     9481 tggaacacca tgcttaagtt attctctgca ttcagaaaaa ataaaatctg ggatttcaac
     9541 ggcggcatcc atccaccgga gatgaaaacc cagaccaacg gtacccccct gcgccaggta
     9601 ctactggcgc agcattttgt tattccactg aaacagcata ttggcgctga aggtgagttg
     9661 tgcgttagcg tcggcgataa agtattgcgc ggccagccgc ttacccgtgg tcgcggcaaa
     9721 atgctgcctg ttcacgcgcc cacctcgggt accgttacgg ctattgcgcc ccactctacg
     9781 gctcatcctt cagctttagc tgaattaagc gtgattattg atgccgatgg tgaagactgc
     9841 tggatcccgc gcgacggctg ggtcgattat cgctctcgca gtcgcgaaga gttaatcgag
     9901 cgcatacatc agtttggtgt tgccgggctg ggcggtgccg gattcccgac aggcgttaaa
     9961 ttgcagggtg gtggagataa gattgaaacg ttgattatca acgctgctga gtgcgagccg
    10021 tacattaccg ccgatgaccg tttgatgcag gattgcgcgg ctcaggtcgt agagggtatt
    10081 cgcattcttg cgcatattct gcagccacgc gaaattctta tcggcattga agataacaaa
    10141 ccgcaggcga tttccatgct gcgcgcggtg ctggcggact ctaacgatat ttctctgcgg
    10201 gtgattccaa ccaaatatcc ttctggcggt gctaaacaat taacctacat tctgaccggg
    10261 aagcaggttc cacatggcgg gcgttcatcc gatatcggcg tattaatgca aaacgtcggc
    10321 actgcttatg cagtgaaacg tgccgttatt gatggcgagc cgattaccga gcgtgttgta
    10381 accctgactg gcgaagcaat cgctcgcccg ggcaacgtct gggcacggct ggggacgcca
    10441 gtgcgtcatt tattgaatga tgccggattc tgcccctctg ccgatcaaat ggtgattatg
    10501 ggtggcccgc taatgggctt taccttgcca tggctggatg tcccggtcgt aaagattacc
    10561 aactgtctgt tggctccctc tgccaatgaa cttggcgaac cacaggaaga acaaagctgc
    10621 atccggtgta gcgcctgtgc tgacgcctgc cctgtggatc ttttgccgca acagttgtac
    10681 tggttcagca aaggtcagca acacgataaa gctaccacgc ataacattgc cgactgcatt
    10741 gaatgtgggg cttgcgcgtg ggtttgcccg agcaatattc ccttggtgca atatttccgt
    10801 caggaaaaag ctgaaattgc ggctattcgt caggaagaaa agcgcgccgc agaagccaaa
    10861 gcgcgtttcg aagcgcgcca ggctcgtctg gagcgcgaaa aagcgactcg ccttgaacga
    10921 cataagagcg cagccgttca acctgcagcc aaagataaag atgcgattgc tgccgctctg
    10981 gcgcgggtga aagagaaaca ggcccaggct acacagccta ttgtgattaa agcgggcgaa
    11041 cgcccggata acagtgcaat tattgcagaa cgggaagccc gtaaagcgca agccagagcg
    11101 aaacaggcag aactggagca aactaacgac gcagcaaccg ttgctgaacc acgtaaaact
    11161 gccgttgaag cagctatcgc ccgctccaaa gcgcgcaagc tggaacagca acaggctaat
    11221 gcggaaccag aagaacaggt cgatccgcgc aaagccgccg tcgaagccgc tattgcccgt
    11281 gccaaagcgc gcaagctgga acagcaacag gctaatgcgg aaccagaaga acaggtcgat
    11341 ccgcgcaa
//
LOCUS       DAZSNO010000120        11315 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZSNO010000120 DAZSNO010000000
VERSION     DAZSNO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.120"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            207..506
                     /locus_tag="ACHWN2_003023"
     CDS             207..506
                     /locus_tag="ACHWN2_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR8932466.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(626..805)
                     /locus_tag="ACHWN2_003024"
     CDS             complement(626..805)
                     /locus_tag="ACHWN2_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR8932467.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            879..2240
                     /gene="pabB"
                     /locus_tag="ACHWN2_003025"
     CDS             879..2240
                     /gene="pabB"
                     /locus_tag="ACHWN2_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR8932468.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2244..2822
                     /locus_tag="ACHWN2_003026"
     CDS             2244..2822
                     /locus_tag="ACHWN2_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR8932469.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            3006..4370
                     /gene="sdaA"
                     /locus_tag="ACHWN2_003027"
     CDS             3006..4370
                     /gene="sdaA"
                     /locus_tag="ACHWN2_003027"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR8932470.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4501..6099
                     /locus_tag="ACHWN2_003028"
     CDS             4501..6099
                     /locus_tag="ACHWN2_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR8932471.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6103..7653)
                     /gene="yoaE"
                     /locus_tag="ACHWN2_003029"
     CDS             complement(6103..7653)
                     /gene="yoaE"
                     /locus_tag="ACHWN2_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR8932472.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(7641..7799)
                     /gene="yoaL"
                     /locus_tag="ACHWN2_003030"
     CDS             complement(7641..7799)
                     /gene="yoaL"
                     /locus_tag="ACHWN2_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR8932473.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8116..9087
                     /gene="manX"
                     /locus_tag="ACHWN2_003031"
     CDS             8116..9087
                     /gene="manX"
                     /locus_tag="ACHWN2_003031"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR8932474.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9150..9950
                     /gene="manY"
                     /locus_tag="ACHWN2_003032"
     CDS             9150..9950
                     /gene="manY"
                     /locus_tag="ACHWN2_003032"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR8932475.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9963..10814
                     /gene="manZ"
                     /locus_tag="ACHWN2_003033"
     CDS             9963..10814
                     /gene="manZ"
                     /locus_tag="ACHWN2_003033"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR8932476.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10869..>11315
                     /locus_tag="ACHWN2_003034"
     CDS             10869..>11315
                     /locus_tag="ACHWN2_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR8932477.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
BASE COUNT         2783 a         2857 c         2847 g         2828 t
ORIGIN      
        1 tagctgaaca atctggttat agcgtgcggg ctgaccgtta attttctcca tcttactgga
       61 taacacacca tgagctgcta cctttaaaac acctgaatgt caacaggtta actcgtgcca
      121 tatcgttata atccctttga tattagatgc aaattaaggt catatatagc cttatcgata
      181 acatggttaa ttttaaggat aagaatatgc ctgctgtaat agataaagcc ctggatttca
      241 ttggtgccat ggatgtatca gcgccaacac caagttcgat gaatgaaagc acggcgaagg
      301 gaatctttaa atatttaaaa gaactgggag tacccgcgag tgccgctgat attacggcgc
      361 gagctgacca ggaaggctgg aaccctgggt tcacggaaaa aatggttgga tgggcaaaaa
      421 aaatggagac aggtgaacgc tctgtgatta aaaatcctga atacttttca acatatatgc
      481 aggaagaact aaaagcactg gtctgagtta aatttatatc agcataaatg ggtcaggacg
      541 cttttaatca catataaaaa gcgtcctttt tctcacaatc aaatgatcat cggcttctat
      601 aacgaaatcg atacggatta gtgtgttact cgtcttcatc ctcaaaacgc gccacgatta
      661 gctcgccgga gtggttggca cgcagctctt ctgccaccag cgcgattgcc tggccgctac
      721 tcatcccttg tgccatcagt tcctggatcc gctcgacagc tttttgctgc tgttcatgga
      781 tgagtgaagg taaacctgca aacattgtta actcctgcta aattgttggc gctaattatt
      841 tcatgctacc cggcacatag ccagtagagt caggactgat gaagacgtta tctcccactg
      901 tgattacttt accctggcgt caggacgccg ctgaatttta tttctcccgc ttaagccacc
      961 tgccgtgggc gatgctttta cactccggct atgccgatca tccgtatagc cgctttgata
     1021 ttgtggtcgc cgatccgatt tgcactttaa ccactttagg taaagaaacc gttgttagtg
     1081 aaagcgaaaa acgcacaacg accactgatg acccgctaca ggtgctccag caggtgctgg
     1141 atcgcgcaga cattcgccca acgcataacg aagatttgcc atttcagggc ggcgcactgg
     1201 ggttgtttgg ctacgatctg ggccgccgtt ttgagtcact gccagaaatt gcggaacaag
     1261 atatcgttct gccggatatg gcagtgggta tctacgattg ggcgctcatt gtcgaccacc
     1321 agcgtcatac agtttctttg ctgagtcata atgatgtcaa tgcccgtcgg gcctggctgg
     1381 aaagccagca attctcgccg caggaagatt tcacgctcac ttccgactgg caatccaata
     1441 tgacccacga gcagtacggc gaaaaatttc gccaggtaca ggaatatctg cacagcggtg
     1501 attgctatca ggtgaatctc gcccagcgtt ttcatgcgac ctattctggc gatgaatggc
     1561 aggcattcct tcagcttaat caggccaacc gcgcgccatt tagcgctttt ttacgtcttg
     1621 aacagggtgc aattttaagc ctttcgccag agcggtttat tctttgtgat aatagtgaaa
     1681 tccagactcg cccgattaaa ggcacgctac cacgcctgcc cgatcctcag gaagatagca
     1741 aacaagcaga aaaactggcg aactcagcga aagatcgtgc cgaaaatctg atgattgtcg
     1801 atttaatgcg taatgatatc ggtcgtgttg ccgtagccgg ttcggtaaaa gtaccagagc
     1861 tcttcgtggt ggaacccttc cctgccgtgc atcatctggt cagcactata acggcgcgac
     1921 taccagaaca gttacacgcc agcgatctgc tgcgcgcagc ttttcctggt ggctcaataa
     1981 ccggggctcc gaaagtacgg gctatggaaa ttatcgacga actggaaccg cagcgacgta
     2041 atgcctggtg cggcagcatt ggctatttga gcttttgcgg caacatggat accagcatta
     2101 ctatccgcac gctgactgcc attaacggac aaatatactg ctctgcgggc ggtggaattg
     2161 tcgccgatag ccaggaagaa gcggaatatc aggaaacttt tgataaagtt aataagatat
     2221 tacgccaact ggagaagtaa gacgtggaat accgtagcct gacgcttgat gattttttat
     2281 ctcgctttca acttttgcgc ccgcaaatta accgggaacc cctaaatcat cgtcaggctg
     2341 ccgtgttaat ccccatcgtc cgtcgaccgc aaccggggtt gttgctgact cagcgttcaa
     2401 ttcatctgcg taaacacgct ggacaagtgg cattccctgg aggtgcagtc gatgacacgg
     2461 acgcatcagt tatcgccgcc gcgctgcgcg aagctgaaga agaggtcgct ataccgcctt
     2521 ccgccgttga agttatcggc gtgctgccgc ccgtcgatag cgtaactggc tatcaagtaa
     2581 ccccagtggt cggcattatc ccgcccgatc tgccgtatcg cgccagtgaa gatgaagtct
     2641 cggcggtgtt tgaaatgccg ctcgcccagg cattacatct gggtcgttat caccctttag
     2701 atatctaccg ccgtggcgat tcacatcggg tatggctatc ctggtacgaa cagtattttg
     2761 tatggggaat gaccgcaggc ataattcgtg agctggcgct gcaaattggt gtgaccccct
     2821 gactatactt atctttacat ctacaaaaca ctacttgaga caatcatcgc aatattagtt
     2881 aaatcgcggt ttttgattag tttaattcat gtgaatagtt aagccagtcg ccgcgttccc
     2941 tcttacacta tgcgctgtta ttagttcgtt actggaagtc cagtcacctt gtcaggagta
     3001 ttatcgtgat tagtctattc gacatgttta aggtggggat tggtccctca tcttcccata
     3061 ccgtagggcc tatgaaggcg ggtaaacagt tcgtcgatga tctggtcgaa aaaggcttac
     3121 tggatagcgt tactcgcgtt gccgtggacg tttatggttc actgtcgctg acgggtaaag
     3181 gccaccacac cgatatcgcc attattatgg gtcttgcagg taacgaacct gccaccgtgg
     3241 atatcgacag tattcccggt tttattcgcg acgtaaaaga gcgcgaacgt ctgctgctgg
     3301 cacagggacg gcatgaagtg gatttcccgc gcgacaacgg gatgcgtttt cataacggca
     3361 acctgccgct gcatgaaaac ggtatgcaaa tccacgccta taacggcgat gaagtcgtct
     3421 acagcaaaac ttattattcc atcggcggcg gttttatcgt cgatgaagaa cactttggtc
     3481 aggatgctgc caacgaagtg agcgtgccgt atccgttcaa atctgccacc gaactgctcg
     3541 cgtactgtaa tgaaaccggc tattcgctgt ctggtctcgc tatgcagaac gaactggcgc
     3601 tgcacagcaa gaaagagatc gacgagtatt tcgcgcatgt ctggcaaacc atgcaggcat
     3661 gtatcgatcg cgggatgaac accgaaggtg tactgccagg cccgctgcgc gtgccacgtc
     3721 gtgcgtctgc cctgcgccgg atgttagttt ccagcgataa actgtctaac gatccgatga
     3781 atgtcattga ctgggtaaac atgtttgcgc tggcagttaa cgaagaaaac gccgccggtg
     3841 gtcgtgtggt aactgcgcca accaacggtg cctgcggtat cgttccggca gtgctggctt
     3901 actatgacca ctttattgaa tcggtcagcc cagacatcta tacccgttac tttatggcag
     3961 cgggcgcgat tggtgcattg tataaaatga acgcctctat ttccggtgcg gaagttggtt
     4021 gccagggcga agtgggtgtt gcctgttcaa tggctgctgc gggtcttgca gaactgctgg
     4081 gcggtagccc ggaacaggtt tgcgtggcgg cggaaattgg catggaacac aaccttggtt
     4141 taacctgcga cccggttgca gggcaggttc aggtgccgtg cattgagcgt aatgccattg
     4201 cctctgtgaa ggcgattaac gccgcacgga tggctctgcg ccgcaccagt gcaccgcgcg
     4261 tctcgctgga taaggtcatc gaaacgatgt acgaaaccgg taaggacatg aacgccaaat
     4321 accgcgaaac ctcacgcggt ggtctggcaa tcaaagtcca gtgtgactaa tacttcttac
     4381 tcgcccatct gcaacggatg ggcgaattta tacccgcttt ctcgtctgct gtaatattcc
     4441 ccactacact tccactgttg cgtcaggcgt ttgtcgccat acgcttacag ggtggcccgc
     4501 atgcaaaaag cacaacggat cattaaaacc tatcgccgta atcgaatgat tgtttgtacg
     4561 atttgcgcac tcgttacgct cgcttcgacc ctgagcgtgc gatttatttc acagcgtaac
     4621 ttaaatcaac aacgggtagt acaattcgcc aatcacgctg tagaggaatt agataaagta
     4681 ctgcttcccc tacaggcagg tagcaaagtc ttgcttccgc tgattggtct gccctgctct
     4741 gtcgcccatt tgccattacg taaacaggcg gcaaaactcc aaactgtgcg atccattggc
     4801 ctggtgcaag acggcacact ttattgctcc agcatttttg gttatcgcaa tgtgcccgtc
     4861 gtggacattc tggctgaact tcctgcaccg caaccacttt tacgcctgac gatcgaccgt
     4921 gccctgatta aaggcagtcc ggttttgatt caatggacgc ctgcagcggg cagtagcaat
     4981 gctggggtca tagagatgat taacatcgac ttactgacgg caatgctcct tgagccacaa
     5041 ctgccgcaaa tcagtagcgc cagcctgacg gtggacaaac ggcatttgct ctatggtaat
     5101 gggctggtag attcccttcc gcaacctgaa gacaatgaaa actatcaggt ttcttcgcaa
     5161 agctttcctt ttaccattaa cgtaaatggt ccgggggcta cggcgctggc atggcactat
     5221 cttccaacac aattaccgct ggcggtgctg ctaagtttac tggtgggcta tatcgcctgg
     5281 ctggcgaccg cttaccggat gagcttttcc cgcgaaatta atctgggcct ggcgcaacat
     5341 gagttcgaat tgttctgtca gcctttgctt aatgcgcgca gccagcaatg tattggtgta
     5401 gagattttgc tgcgctggaa taatccgcgt cagggctgga tttcaccgga tgtgtttatt
     5461 cctatcgcgg aagaacatca tttaattgtg ccactgaccc actatgtgat ggcagaaacc
     5521 attcgtcagc accatgtttt cccgatgagt agtcagtttc atgttggcat taacgtcgca
     5581 cccagccatt ttcgccgtgg tgtgctgata aaagatctca atcagtactg gtttagcgct
     5641 cacccgattc agcaactgat cctcgaaatc accgaacgcg atgccttact ggatgttgat
     5701 tatcggattg cccgcgagct gcatcgtaaa aacgtcaaac tggcgattga tgacttcggc
     5761 accggcaaca gctcgttttc ctggcttgaa acattacgtc ctgacgtgct gaaaattgat
     5821 aagtcattta ccgcagctat aggttctgac gcggttaact cgacggtgac cgatatcatc
     5881 atcgcgctgg ggcaaagact gaatattgaa ctggtggcgg agggcgtgga aacgcaagaa
     5941 caggcgaagt atttgcgccg tcatggcgtg catattttgc aagggtattt gtacgcacag
     6001 ccgatgccgc tacgtgattt tcccaaatgg ctggcgggca gccaaccgcc gcccgcccgg
     6061 cataatggac atatcacgcc cattatgtcg ttacgttaac gcttactcat cttcatcgtg
     6121 cgccggttgc tctttaacaa tgcgaaccag atcaacacga taatcattgg cttcaaagat
     6181 ggtgatatgc agtggcccta catcaatcac atcgcccaca cggggaatgt gaccatttgc
     6241 cgagatcacg aggcccgcga ccgtcgcgat atcatcgtca tcggcaaggt gctcaacatc
     6301 aagcgcctgc tgcaaggcat gcaaatctgt accgcctttt accagccagc cgttaccatc
     6361 ggtaatgatt tccggcgttt cgtcagcgtc cgggaattca cccgcaatgg cttccagcac
     6421 atccagcggc gtgaccaagc cttgtaccac accaaactcg ttggtcacga taacaaagct
     6481 cccgcgagcg cgacgcagca cgcccagcag gttgatcgga tcgagggttt ccgggacgat
     6541 aatcgccgga gacgccgaag caatcgccgc cacatcaacg ccctcttcca gcgccaccag
     6601 cagttcttta gcacgtacga taccgatgat ttcatccagt tcaccgcgac ataccgggaa
     6661 cagactgtgc ggtgaagaga gcagttgctc gcggatttca tcgaccccga gattagcgtc
     6721 aacccagctt atttcaccgc gcggcgtcat gatcccgcgc agagaacgcg acgccagcgt
     6781 cagtacgccg ttaatcatgt accgttcttc ttcggcaaat gcaccttccg ggatcggcat
     6841 cgggttatcg gcatcgtgct gaacattggc ctgacgtttc ccgcccatca aacgcaggat
     6901 ggcatcggca gtacgcgctc gcagcggcaa agtcgactgg tgacgaataa agttgcgacg
     6961 cgcaatctgg ttaaacactt cgataatgat cgagaagcca atcgcggcat acaggtaacc
     7021 tttcggaatg tggaaaccga aaccttctgc caccagactc agaccaatca ttaacaggaa
     7081 gctcagacag agcaccacca ccgtggggtg ctggttaacg aatcgcgtca gcggtttcga
     7141 tgccagcaac ataaccgcca tcgcaatcac taccgccgcc atcatcaccg gcagatggtt
     7201 aaccatccct actgcagtaa ttaccgcatc caacgagaag acggcgtcaa ggatgacaat
     7261 ctgtgtgacg accacccaga aactggcgta gcctttaccg tggccggaat catgatcgcg
     7321 gttttccagc cgttcatgca gttcggttgt tgctttgaac agcaagaata tccccccgaa
     7381 caacataatc agatcgcgtc cggagaagga gaaatccatg acggtaaata gcggtttggt
     7441 cagcgtgacc atccatgaaa tcagcgacag cagccccaga cgcataatca gcgccagtga
     7501 taaccccagc aaacgcgctt tatcgcgttg ttttggcggc agtttgtcag caagaatggc
     7561 gatgaagacc aggttatcga tacccagcac aatttcgaga acaacaagcg tgagtagccc
     7621 cgcccaaatt gaggggtcca ttaagaattc catgacaagc tcctgcttaa ggaatagcta
     7681 ttcgacgcca gaaataatgc aggcgtaacg acaaaatgca aacgaaaggt gcggcataga
     7741 gtgccagaaa ggcaggcgtt aaaaggcctg atgctgaaat gacgtcggtg acgatccata
     7801 ctgcgggcta ctgccctata ctccatggtt gttaaacggg agttaaacat atcagagacg
     7861 cctctgattt ggcaaagatt taccttcctt tgcaaacgaa tgtgacaagg atattttacc
     7921 tttcgaaatt tctgataatc gaaagttaaa ttacggatct tcatcacata aaataatttt
     7981 ttcgatatct aaaataaatc gcgaaacgca ggggtttttg gttgtagccc ttatctgaat
     8041 cgattcgatt gtggacgacg attcaaaaat acatctggca cgttgaggtg ttaacgataa
     8101 taaaggaggt agcaagtgac cattgctatt gttataggca cacatggttg ggctgcagag
     8161 cagttgctta aaacggcaga aatgctgtta ggcgagcagg aaaacgtcgg ctggatcgat
     8221 ttcgttccag gtgaaaatgc cgaaacgctg attgaaaaat acaacgctca gttggcaaaa
     8281 ctcgacacca ctaaaggcgt gctgtttctc gttgatacat ggggaggcag cccgttcaat
     8341 gctgccagcc gcattgtcgt cgacaaagag cattatgaag tcattgcagg cgttaacatt
     8401 ccaatgctcg tggaaacgtt aatggcccgt gatgatgacc cgagctttga tgaactggtt
     8461 gcgctggcag tagaaacagg ccgtgaaggc gtgaaagcac tgaaagccaa accggttgaa
     8521 aaagccgcgc cagcacccgc tgccgcagca ccaaaagcgg ctccaactcc ggcaaaacca
     8581 atggggccaa acgactacat ggttattggc cttgcgcgta tcgacgaccg tctgattcac
     8641 ggtcaggtcg ccacccgctg gaccaaagaa accaatgtct cccgtattat tgttgttagt
     8701 gatgaagtgg ctgcggatac cgttcgtaag acactgctca cccaggttgc acctccgggc
     8761 gtaacagcac acgtagttga tgttgccaaa atgattcgcg tctacaacaa cccgaaatat
     8821 gctggcgaac gcgtaatgct gttatttacc aacccaacag atgtagagcg tctcgttgaa
     8881 ggcggcgtga aaatcacctc tgttaacgtc ggtggtatgg cattccgtca gggtaaaacc
     8941 caggtgaata acgcggtttc ggttgatgaa aaagatatcg aggcgttcaa aaaactgaat
     9001 gcacgcggta ttgagctgga agtccgtaag gtttccaccg atccgaaact gaaaatgatg
     9061 gatctgatca gcaaaatcga taagtaacgt attgtgttga ttatcattca gttttcacac
     9121 ttaagtctta cgtaaacagg agaagtacaa tggagattac cactcttcaa attgtgctgg
     9181 tatttatcgt agcctgtatc gcaggtatgg gatcaatcct cgatgaattt cagtttcacc
     9241 gtccgctaat cgcgtgtacc ctggtgggta tcgttcttgg ggatatgaaa accggtatta
     9301 ttatcggtgg tacgctggaa atgatcgcgc tgggctggat gaacatcggt gctgcagttg
     9361 cgcctgacgc cgctctggct tctatcattt ctaccattct ggttatcgca ggtcatcaga
     9421 gcattggtgc aggtatcgca ctggcaatcc ctctggccgc tgcgggccag gtactgacca
     9481 tcatcgttcg tactattacc gttgctttcc agcacgctgc ggataaggct gctgataacg
     9541 gcaacctgac agcgatttcc tggatccacg tttcttctct gttcctgcaa gcaatgcgtg
     9601 tggctattcc ggccgtcatc gttgcgctgt ctgttggtac cagcgaagta cagaacatgc
     9661 tgaatgcgat tccggaagtg gtgaccaatg gtctgaatat cgccgggggc atgatcgtgg
     9721 tggttggtta tgcgatggtt atcaacatga tgcgtgctgg ctacctgatg ccgttcttct
     9781 acctcggctt cgtaaccgca gcattcacca actttaacct ggttgctctg ggtgtgattg
     9841 gtactgttat ggcagtgctc tacatccaac ttagcccgaa atacaaccgc gtagccggtg
     9901 cgcctgctca ggcagctggt aacaacgatc tcgataacga actggactaa caggtgagcg
     9961 aaatggttga tacaactcaa actaccaccg agaaaaaact cactcaaagt gatattcgtg
    10021 gcgtattcct gcgttctaac ctcttccagg gttcatggaa cttcgaacgt atgcaggcac
    10081 tgggtttctg cttctctatg gtaccggcaa ttcgtcgcct ctaccctgag aacaacgaag
    10141 ctcgtaaaca agctattcgc cgtcacctgg agttctttaa cacccagccg ttcgtggctg
    10201 caccgattct cggcgtaacc ctggcgctgg aagaacagcg tgctaatggc gcagagatcg
    10261 atgacggtgc tatcaacggt atcaaagtcg gtttgatggg gccgctggct ggtgtaggcg
    10321 acccgatctt ctggggaacc gtacgtccgg tatttgcagc actgggtgcc ggtatcgcga
    10381 tgagcggcag cctgttaggt ccgctgctgt tcttcatcct gtttaacctg gtgcgtctgg
    10441 caacccgtta ctacggcgta gcgtatggtt actccaaagg tatcgatatc gttaaagata
    10501 tgggtggtgg cttcctgcaa aaactgacgg aaggggcgtc tatcctcggc ctgtttgtca
    10561 tgggggcatt ggttaacaag tggacacatg tcaacatccc gctggtggtt tcgaccatca
    10621 ccgggcagga tggacagacc cgtgtcacca ccgtgcaaac tattcttgac cagttaatgc
    10681 caggccttgt accactgctg ctgacatttg cttgtatgtg gctgctgcgc aaaaaagtta
    10741 acccgctgtg gatcatcgtt ggcttcttcg tcatcggtat cgctggttac gcttgcggcc
    10801 tgctgggact gtaagactgt cgtacactac cggggccttt tggccccgtt tttttatctg
    10861 gaggattaat gacaatcacg gacctggtac tgattctttt catcgccgca ctcctggcct
    10921 tcgcgatcta cgatcagttc atcatgcccc gccgtaacgg ccccaccctg ctggcaattc
    10981 ctttgctccg gcgtggtcgc atcgatagcg ttatcttcgt cggattgatt gtcattctta
    11041 tctataacaa cgtcacgaat catggcgcgc taataacaac atggttatta agtgcactgg
    11101 ctctgatggg tttttatata ttctggatcc gcattccgaa gatcatcttt aaacaaaaag
    11161 gttttttctt cgccaatgtc tggattgaat atagccgaat caaagcgatg aacttgtcgg
    11221 aagatggcgt gttggtgatg caattagaac agcgtcggct gttaatccgc gttcgaaata
    11281 tcgacgatct ggaaaaaatt tataagcttc tcgta
//
LOCUS       DAZSNO010000121        11296 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZSNO010000121 DAZSNO010000000
VERSION     DAZSNO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11296
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.121"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="ACHWN2_003035"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="ACHWN2_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR8932478.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /locus_tag="ACHWN2_003036"
     CDS             323..1954
                     /locus_tag="ACHWN2_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR8932479.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="ACHWN2_003037"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="ACHWN2_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR8932480.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /gene="dcuB"
                     /locus_tag="ACHWN2_003038"
     CDS             3241..4581
                     /gene="dcuB"
                     /locus_tag="ACHWN2_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR8932481.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /gene="fumB"
                     /locus_tag="ACHWN2_003039"
     CDS             4659..6305
                     /gene="fumB"
                     /locus_tag="ACHWN2_003039"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR8932482.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6428..7057
                     /locus_tag="ACHWN2_003040"
     CDS             6428..7057
                     /locus_tag="ACHWN2_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR8932483.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7196..8617)
                     /gene="melB"
                     /locus_tag="ACHWN2_003041"
     CDS             complement(7196..8617)
                     /gene="melB"
                     /locus_tag="ACHWN2_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR8932484.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8720..10075)
                     /gene="melA"
                     /locus_tag="ACHWN2_003042"
     CDS             complement(8720..10075)
                     /gene="melA"
                     /locus_tag="ACHWN2_003042"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR8932485.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10358..11266
                     /gene="melR"
                     /locus_tag="ACHWN2_003043"
     CDS             10358..11266
                     /gene="melR"
                     /locus_tag="ACHWN2_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR8932486.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         2953 a         2908 c         2742 g         2693 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtcacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctacgtccg gcacaccaga
     4861 aacaggttgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaacaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc gcctggcagc atgctgccag gtgatccccc tgggccacct
     6361 cttttgcgat tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga
     6421 actcaccatg acccgcacac tcaagccgtt aattcttaac actggcgcac tggcgctaac
     6481 gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt
     6541 gacaccggtg attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac
     6601 gctgctgctc tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta
     6661 cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa
     6721 tccgtatgac aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga
     6781 aattctcgtt cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgtttc tggtctgttg
     6841 cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat
     6901 gggccaggga gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga
     6961 tatgttctgc gcgctgcttg gcgcattaac gacagtgata ttgctcgctc gctttcattg
     7021 ccgccagcta cggcgctatg gcttgataac tggataacaa cgtgcacttt gttgatgccg
     7081 gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa
     7141 gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg
     7201 ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt
     7261 tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat
     7321 agaaacggaa gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa
     7381 taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca
     7441 tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag
     7501 tctgcacgga gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa
     7561 cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca
     7621 aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga
     7681 gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata
     7741 atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg
     7801 acagataata ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa
     7861 atccggtaat aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa
     7921 gctgatcgtt tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag
     7981 cagacgattg attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg
     8041 aaacaataaa aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc
     8101 caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag cctgccagac
     8161 tggcaaaaaa acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg
     8221 ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt
     8281 aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga
     8341 gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg aacttacccc
     8401 atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga
     8461 tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt
     8521 agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa
     8581 atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca
     8641 acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc
     8701 agtagcgttt agtcgcgttt taacggtgca accagcttgg cagccagtcg ccgtgggcgg
     8761 caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg
     8821 ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca
     8881 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac
     8941 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     9001 cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct
     9061 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     9121 tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     9181 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta
     9241 gcttaataaa ccacggcgta tattcggcaa aatgttctga tgattccgtg acgaagtagc
     9301 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat
     9361 ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt
     9421 aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg
     9481 cgcaacggta acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg
     9541 ttccctgcac cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg
     9601 cccaggtatt catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt
     9661 ccgtcatgtt ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     9721 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     9781 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     9841 catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     9901 aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca
     9961 tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt
    10021 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
    10081 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct
    10141 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
    10201 atgggaataa attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct
    10261 cgcccctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc
    10321 tgccataatt ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg
    10381 cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat
    10441 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
    10501 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
    10561 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
    10621 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
    10681 ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca
    10741 gcaacttagc ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca
    10801 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag
    10861 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
    10921 tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct
    10981 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
    11041 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
    11101 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
    11161 tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca
    11221 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
    11281 tcagccaaaa aaatag
//
LOCUS       DAZSNO010000122        11232 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZSNO010000122 DAZSNO010000000
VERSION     DAZSNO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.122"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            104..1245
                     /gene="yghO"
                     /locus_tag="ACHWN2_003044"
                     /pseudo
     CDS             104..1245
                     /gene="yghO"
                     /locus_tag="ACHWN2_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            complement(1369..2139)
                     /gene="glcC"
                     /locus_tag="ACHWN2_003045"
     CDS             complement(1369..2139)
                     /gene="glcC"
                     /locus_tag="ACHWN2_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFR8932487.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2390..3889
                     /gene="glcD"
                     /locus_tag="ACHWN2_003046"
                     /pseudo
     CDS             2390..3889
                     /gene="glcD"
                     /locus_tag="ACHWN2_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            3889..4941
                     /gene="glcE"
                     /locus_tag="ACHWN2_003047"
     CDS             3889..4941
                     /gene="glcE"
                     /locus_tag="ACHWN2_003047"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFR8932488.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4952..6171
                     /gene="glcF"
                     /locus_tag="ACHWN2_003048"
                     /pseudo
     CDS             4952..6171
                     /gene="glcF"
                     /locus_tag="ACHWN2_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6176..6580
                     /locus_tag="ACHWN2_003049"
     CDS             6176..6580
                     /locus_tag="ACHWN2_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFR8932489.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            6602..8773
                     /gene="glcB"
                     /locus_tag="ACHWN2_003050"
     CDS             6602..8773
                     /gene="glcB"
                     /locus_tag="ACHWN2_003050"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFR8932490.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9128..10714
                     /locus_tag="ACHWN2_003051"
     CDS             9128..10714
                     /locus_tag="ACHWN2_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HFR8932491.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         2641 a         2778 c         3114 g         2699 t
ORIGIN      
        1 actatttatt cgtcgtttgt attcattgag ttgattatta agatgccgct cgttgctgtt
       61 gcaacctgtt tttcaccatt aattatttgt tatccacaga gatatggaat gcgatctgct
      121 gatgattaaa attgaaaaag taattaataa aaatgacctt aaggcattta tcgcttttcc
      181 gtcatcactt tatcccgacg atccaaactg gataccccct ttattcattg aacgcaacga
      241 gcatttgtct gcgaaaaatc cggggacgga ccatatcatc tggcaggcgt gggtggcaaa
      301 aaaagcgggg cagatagttg ggcgtattac cgcgcaaata gataccttgc accgtgagcg
      361 ttacggtgaa gataccggtc atttcggcat gattgacgcc attgatgatc cgcaggattt
      421 tgccgcgttg tttggcgcag cggaagcgtg gttgaagtca caaggtgcaa gtaagatcag
      481 cgggcctttc agcctgaata tcaatcagga aagcggatta ctgattgaag gttttgacac
      541 accaccctgt gcgatgatgc cacacggcaa accgtggtat gccgcgcata ttgaacaact
      601 gggttatcac aaaggcattg atttactggc gtggtggatg cagcgaaccg atctcacttt
      661 ctctccggcg ctaaaaaaac tgatggatca ggtgcgcaaa aaggtgacca ttcgctgcat
      721 caatcgtcag cggtttgccg aagagatgca gatcctgcgt gagattttca actctggctg
      781 gcagcacaac tggggatttg tgccgtttac cgaacatgaa ttcgcgacca tgggcgacca
      841 acttaaatat ctggtgccgg acgatatgat ctacatcgct gagattgatt ctgcaccctg
      901 cgcgtttatt gtggcttgcc taacatcaac gaggcgattg ccgatctgaa cggatcgctc
      961 tttcccttcg gctgggcaaa attgctgtgg cgtttgaaag tcagcggtgt gcgaactgcg
     1021 cgagtaccgc tgatgggcgt gcgcgacgag tatcagttca gccgcatcgg cccggtgatt
     1081 gcgctgttat tgattgaagc cttacgcgat ccgtttgccc gccggaagat cgatgcgctg
     1141 gagatgtcat ggatcctcga aaccaataca ggcatgaata acatgctgga acgcattggg
     1201 gcggagccgt acaagcgtta ccgattgtat gaaaagcaaa tttaatacca gatcctgttt
     1261 agcgcagggg cggtcattcg ggagagggta tagatgaaca gatttaacac cagatcctgt
     1321 taagtgcagg ggcggtcccc tcgccttggg gatagggtaa aactctgctc acattcccac
     1381 cttgttcatc tccagcggca ccgccgagcg aatcaaatgg tggccttcca gctcgatatc
     1441 gtggagattc tttttcacgg tccgtacatg atcgcgcgct gcgcgctgag cgacgtgcgg
     1501 cagccgctgc aacaccgcgt tgtagatccg cgcatgctgg cgatcgatct gctttttttg
     1561 ctgtggtcga tggtagagat tatttacgct ggcaaacact gaattaaaca tcagatcggt
     1621 caatgattgc agcgtaaaca ccagcacctg attgtgagaa gcctgacaaa tggcgagatg
     1681 gaaagcgtga tccaactgcg catgttcgat cagcgaaatc tctttgtggt tctcactggc
     1741 ggcgagcatt ttttcataac agcgggttat cacaacaaaa tcagcctgcg ttcccagcgt
     1801 tgccgccagc cttgccgatt cgccctccag taatgcgcga acgtcgagca gatcgtacag
     1861 cgttcgcggc tgcgtactga acagatggat cagcgggctg gtgtcctgct cccgattaag
     1921 ccgtgcgaca tgagaatcgc gaccctgcgc cgtttcaata atcccgcgcc cgcgcagcac
     1981 ggtcagccct tcacgcagtg cggagcgtga gaagccgagc ttttcacaca gtcgacgttc
     2041 cgagggaagc ggctgaccga ccttcagtac gccgtcgata attaaccgtt cgatactctc
     2101 tgcaaccact tcgcaaatag ggcgacgttc atctttcatt cccggacctc gtgcacaggt
     2161 aggaccaatt tttctgttct taactcgcaa aacacgcaca tcacgcaagt gattatgtta
     2221 catcaattta acattaagtt aaccaagaca aggtcacaga gctggaaaaa aaatggtctg
     2281 accggtagct aaagagatag acgaaaacga aaagcccgct taataactgt tcacagaagc
     2341 agcgcgcaaa aatcagctgc cacacaacac aacaaagcga agcctactca tgagcatctt
     2401 gtacgaagag cgtcttgatg gcgctttacc cgatgtcgac cgcacatcgg tactgatggc
     2461 actgcgtgag catgtccctg gacttgagat cctgcatacc gatgaggaga tcattcctta
     2521 cgagtgtgac gggttgagcg cgtatcgcac gcgtccatta ctggttgttc tgcctaagca
     2581 aatggaacag gtgacagcga ttctggctgt ctgccatcgc ctgcgtgtac cggtggtgac
     2641 ccgtggtgca ggcaccgggc tttctggtgg cgcgctgccg ctggaaaaag gtgtgttgtt
     2701 ggtgatggcg cgctttaaag agagcctcga cattaacccc gttggtcgcc gcgcgcgcgt
     2761 gcagccgggc gtatgtaacc tggcgatctc ctaggccgtt gcgccgcata atctctatta
     2821 cgcgccggat ccttcctcac aaatcgcctg ttcaattggc ggcaatgtgg cggaaaatgc
     2881 cggtggcgtc cactgcctga aatatggtct gaccgtacat aacctgctga aaattgaagt
     2941 gcaaacgctg gacggcgagg cgctgacgct gggatcggac gcgctggatt cacctggttt
     3001 tgacctgctg gcgctgttca ccggatcgga aggtatgctc ggcgtgacca ccgaagtgac
     3061 ggtaaaactg ctgccgaagc cgcccgtggc gcgggttctg ttagccagct ttgactcggt
     3121 agaaaaagcc ggacttgcgg ttggtgacat catcgccaat ggcattatcc ccggcgggct
     3181 ggagatgatg gataacctgt cgatccgcgc ggcggaagat tttattcatg ccggttatcc
     3241 cgtcgacgcc gaagcgattt tgttatgcga actggacggc gtggagtctg acgtacagga
     3301 agactgcgag cgggttaact acatcttgtt gaaagcgggc gcgactgacg tccgtttggc
     3361 acaggacgaa gcagagcgcg tacgtttctg ggccggtcgc aaaaatgcgt tcccggcggt
     3421 aggacgtatc tccccggatt actactgcat ggatggcacc atcccgcgtc gcgccctgcc
     3481 tggcgtactg gaaggcattg cccgtttatc gcagcaatat gatttacgtg ttgccaacgt
     3541 ctttcatgcc ggagatggca acatgcaccc gttaatcctt ttcgatgcca acgaacctgg
     3601 tgaatttgcc cgcgcggaag agctgggcgg gaagatcctc gaactctgcg ttgaagttgg
     3661 cggcagcatc agtggggaac atggcatcgg gcgagaaaaa atcaatcaaa tgtgcgccca
     3721 gttcaacagc gatgaaatca cgaccttcca tgcggtcaag gcggcgtttg accccgatgg
     3781 tttgctgaac cctgggaaaa acattcccac gctacaccgc tgtgctgaat ttggtgccat
     3841 gcatgtgcat cacggtcatt taccttttcc tgaactggag cgcttttgat gctacgcgag
     3901 tgtgattaca gtcaggcgct gctggagcag gtgaatcagg caattagcga taaaacgccg
     3961 ctggtgattc agggcagcaa tagcaaagcc tttttaggtc gcccagtcac cgggcaaacg
     4021 ctggatgtgc gttgtcatcg cggcattgtt aattacgacc cgaccgagct ggtgataacc
     4081 gctcgtgccg gaacgccgct ggtggcgatt gaagcggcgc tggagagcgc cgggcaaatg
     4141 cttccctgcg agccgccgca ttatggtgaa gaagccacct ggggcgggat ggtcgcctgc
     4201 gggctggcgg ggccgcgtcg cccgtggagc ggttcggtcc gcgattttgt cctcggcacg
     4261 cgcatcatta ccggcactgg aaaacatctg cgttttggtg gcgaagtgat gaaaaacgtt
     4321 gccggatacg atctctcacg gttaatggcc ggaagctacg gttgtcttgg cgtgctcact
     4381 gaaatctcaa tgaaagtgtt accgcgaccg cgtgcctctc tgagcctgcg tcgggaaatc
     4441 agcctgcaag aagccatgag tgaaatcgcc gagtggcaac tccagccatt acccattagt
     4501 ggcttatgtt acttcgacaa tgcgttgtgg atccgccttg agggcggcga aggatcggta
     4561 aaagcagcgc gtgaactgct gggtggcgaa gaggttgtgg gtcagttctg gcagcaattg
     4621 cgtgaacaac aactgccgtt cttctcgtta ccaggtacct tatggcgcat ttcattaccc
     4681 agcgatgcgc cgatgatgga tttacccggc gagcaactga tcgactgggg cggggcgtta
     4741 cgctggctga aatcgacagc cgatgacaat caaatccatc gcatcgcccg caacgctggc
     4801 ggtcatgcga cccgctttag tgccggagat ggtggctttg ccccgctatc ggctccttta
     4861 ttccgctatc accagcagct taaacagcag ctcgaccctt gcggcgtgtt taaccccggt
     4921 cgcatgtacg cggaactttg aggagcaggc tatgcaaacc cgattaaccg aagagatgcg
     4981 gcagaacgcg cgctggaagc cgacagcatc ctgtgcgcct gtgttcactg cggattttgt
     5041 accgcaacct gcccaaccta tcagcttctg ggcgatgaac tggacgggcc gcgcgggcgc
     5101 atctatctga ttaaacaggt gctggaaggc aacgaagtca cgcttaaaac acaggagcat
     5161 ctcgatcgct gcctcacttg ccgcaattgt gaaaccacct gtccttctgg tgtgcgctat
     5221 cacaatttgc tggatatcgg gcgtgatatt gtcgagcaga aagtgaaacg cccactgccg
     5281 gagcgaatgc tgcgcgaagg attgcgccag gtagtgccgc gtccggcggt cttccgtgcg
     5341 ctgacgcagg tagggctggt gctgcgaccg tttttaccgg aacaggtcag agcaaaactg
     5401 cctgctgaaa cggtgaaagc taaaccgcgt ccgccgctgc gccataagcg tcgggtttta
     5461 atgctggaag gctgcgccca gcctacgctt tcgcccaaca ccaacgcggc aactgcgcga
     5521 gtgctggatc gtctggggat cagcgtcatg tcagctaacg aagcaggctg ttgtggcgcg
     5581 gtggactatc atcttaatgc gcaggagaaa gggttggcac gggcgcgcaa taatattgat
     5641 gcctggtggc ccgcgattga agcaggtgcc gaggcaattt tgcaaaccgc cagcggttgc
     5701 ggcgcgttcg tcaaagagta tgggcagatg ctgaaaaacg atgcgttata tgccgataaa
     5761 gcgcgtcagg tcagtgaact ggcggtcgat ttagtcgaac ttctgcgcga ggaaccgctg
     5821 gaaaaactgg caattcgcgg cgataaaaaa ctggccttcc actgcccgtg taccctacaa
     5881 catgcgcaaa agctgaacgg cgaagtggaa aaagtgttac ttcgtcttgg atttacctta
     5941 acggacgttc ccgacagcca tctgtgctgc ggttcagcgg gaacatatgc gttaacgcat
     6001 cccgatctgg cacgccagct gcgggataac aaaatgaatg cgctggaaag cggcaaaccg
     6061 gaaatgatcg tcaccgccaa cattggttgc cagacgcatc tggcgagcgc cggtcgtacc
     6121 tctgtgcgtc actggattga aattgtagaa caagcccttg aaaaggaata aaaaaatgaa
     6181 aactaaagtc attcttagcc agcaaatggc gagtgcaatt attgccgcag gtcaggaaga
     6241 ggcgcagaaa aataactggt ctgtttccat tgctgttgcc gatgacggcg gtcatctgct
     6301 ggcgttaagt cgcatggacg attgcgcgcc gattgcggct tacatctccc aggagaaagc
     6361 gcgtaccgcc gcgctggggc gtcgtgaaac taagggctat gaagagatgg tgaacaacgg
     6421 acgtaccgcg ttcgtgactg cgccgttact gacgtcgctg gaaggcggcg taccggttgt
     6481 tgttgatggg caaattattg gtgccgtggg cgtttctggt ttaaccggag cacaggatgc
     6541 gcaggtcgcg aaagtggcag cagcggtgtt ggcgaaataa gcgaaaacga ggagagaaac
     6601 aatgagtcaa accataaccc agggccgttt acgcattgac gccaatttta aacgttttgt
     6661 ggatggagaa gttttgccgg gcgtggaact ggacgctgct gcattctggc acaatgttga
     6721 tgagatcgtt cacgatctgg caccagaaaa tcgtcagttg ctggcagaac gcgatcgcat
     6781 tcaggcggcg cttgatgagt ggcatcgcag caatccgggg ccggtaaaag ataaagcggc
     6841 ctataaatct ttccttcgtg aactgggcta cctggtgccg caaccggagc gcgtgacggt
     6901 ggaaaccacg ggcattgaca gcgaaatcac cagtcaggcg gggccacagc tggtggttcc
     6961 ggcaatgaac gcccgctacg cgctgaacgc ggcgaacgct cgctggggct cactgtacga
     7021 tgcgttatac ggcagcgaca tcatcccgca ggaaggggcg atggtcagtg gctacgatcc
     7081 gcagcgcggt gagcaggtta tcgcctgggt tcgacgtttc ctcgatgaat cactaccgct
     7141 ggaaaacggc agctatcagg atgtggtggc gtttaaggtg gtcgataaac aattacgcat
     7201 ccagttgaaa aatggtaaag aaaccacgtt acgtactcca gcacagtttg tcggttaccg
     7261 tggcgatgcc gctgcgccga cctgcatttt gctgaaaaat aacggcctgc atattgaact
     7321 gcaaattgat gccaacgggc ggattggcaa agacgatccg gcgcacatca acgatgttat
     7381 cgtcgaagct gctatcagta ccattctcga ctgcgaagat tcggtcgcgg cggttgatgc
     7441 ggaagataaa atcctgctgt accgcaatct gctgggcctg atgcagggga ctctgcaaga
     7501 gaaaatggag aaaaacggtc ggcaaatcgt acgtaaactg aatgacgatc gtcagtacac
     7561 cgccgccgat ggctctgaaa tttctctgca cggacgctcg ttgctgttta tccgcaacgt
     7621 gggtcatttg atgaccattc ctgtgatttg ggacagcgaa ggcaatgaaa tcccggaagg
     7681 tattcttgat ggcgtcatga ctggcgcgat tgccctctat gatttaaaag tgcagaaaaa
     7741 ctcgcgcact ggcagcgtct atattgtgaa accgaaaatg cacggtccgc aggaagtggc
     7801 gttcgccaac aaactgttta cccgcattga gacaatgctc ggtatggcac cgaataccct
     7861 gaaaatgggc attatggatg aagaacgccg gacctcgctg aacttgcgta gctgtatcgc
     7921 tcaggcgcgc aaccgcgtgg cgttcatcaa taccggtttc ctcgaccgta ccggcgatga
     7981 aatgcattcg gtgatggaag ctggcccgat gctgcgtaaa aaccagatga aatcgacgcc
     8041 gtggatcaaa gcctacgaac gtaataacgt gctttccggt ctgttctgtg ggctgcgcgg
     8101 taaagcgcaa atcggtaaag gcatgtgggc aatgccggac ctgatggcag acatgtacag
     8161 ccagaagggc gaccaactgc gtgccggggc aaacacagcc tgggttccgt caccaaccgc
     8221 tgctacgctc catgcgctgc actaccacca taccaacgta cagagcgtgc aagccaacat
     8281 tgcccagacc gagttcagtg ctgaatttga accgctgctg gacgatctgc tgactattcc
     8341 ggtggctgaa aacgctaact ggtcggcgca agagatccaa caagagctgg ataacaacgt
     8401 gcaggggatt ctggggtacg tggtgcgttg ggtggagcag gggattggtt gttcaaaagt
     8461 gccggatatt cacaacgtgg cgctgatgga agaccgcgca acgctgcgta tctccagcca
     8521 gcatatcgcc aactggttac gtcacggtat tctgaccaaa gaacaggtgc aggcgtcgct
     8581 ggagaatatg gcgaaagtgg ttgatcagca aaatgctggc gatccggctt atcgtccgat
     8641 ggcggggaat ttcgctaact cgtgtgcttt taaagctgcc agcgatttaa tcttcctcgg
     8701 cgtgaaacag ccaaacggtt ataccgaacc gttattacac gcctggcgtt tacgcgaaaa
     8761 agaaagtcat taatagcgtc aaaaaagccc cggcaagttt tattgtcggg gctatgatag
     8821 taactgcggt agtacattta tcccggaaat aaccggagcg attatcaggg tttaacaata
     8881 ttaaataccc gtagcgcatt actagcgggc ctaaaacaac aatatggaca tcttaacgat
     8941 actctgcgcc tgcgtgtgcg gaacagaaac ccttacccga tatacttgtt atgccagagg
     9001 tagcaattgc gttagcggcg tactgttgct gtaaagtata acgcctatat tttacagttc
     9061 agtcctcttt tgcttattac aaccttaagt atatcaagca tataaagata ataagagact
     9121 gaacaatatg gttacctgga cccaaatgta tatgccgatg ggaggactgg ggctatccgc
     9181 tctggtcgcc ctgatcccga taatattctt cttcgttgca ctcgcggtat tacgtctgaa
     9241 aggacatgtt gctggagcaa taacccttat attatctatc ctgattgcga tattcgcctt
     9301 taaaatgccg attgatatgg catttgctgc tgcgggctat ggctttattt atggattatg
     9361 gccaattgcg tggattattg tcgcggcggt gttcctgtat aaattaaccg ttgccagcgg
     9421 gcagttcgat attatccgca gctcggttat ctccatcacc gacgatcagc gtttgcaggt
     9481 gttactgatt ggtttctcct ttggtgcgtt gctggaagga gctgctggct ttggtgcgac
     9541 ggtggcgttt ggtgcgttgg gcgtgccgat tctggtcgcc ggtcaggtaa cgggaatcga
     9601 tccgttccac attggcgcaa tggcgggacg tcagttaccg ttcctgtcgg ttcttgtgcc
     9661 gttctggctg gtagcaatga tggacggctg gaaaggggtg aaagagacgt ggccagcggc
     9721 gctggttgct gggggaagct tcgctgtcac tcagttcttt acctctaact atattggtcc
     9781 ggaactgccg gatattactt cggcgctggt gagtatcgtc tcactcgctt tattccttaa
     9841 agtctggcgg ccgaaaaata ccgaaacggc aatcagcatg gggcaatccg caggtgcgat
     9901 ggtggtaaat aagccatctt ctggcggtcc cgtgccttca gaatatagtc tggggcaaat
     9961 cattcgtgcg tggtcaccgt ttttaatctt aacggtgctg gtcaccatct ggaccatgaa
    10021 gccgtttaaa gcgttatttg ctccgggcgg cgcgttttat tcactggtga ttaatttcca
    10081 gatccctcat ttgcatcaac aagtgttgaa agcggcaccc attgtcgccc aaccaacgcc
    10141 aatggatgcg gtgtttaaat tcgaccctct ctcggctggc ggcaccgcta tttttattgc
    10201 ggcgattatc tctatcttca tcctcggcgt gggcatcaag aaagggattg gcgtctttgc
    10261 cgaaatgcta attagtctga agtggccgat attgtcgatt ggcatggtgc tggcattcgc
    10321 cttcgtcacc aactattccg gcatgtccac cacgctggcg ctggtactgg caggtacagg
    10381 cgtgatgttc ccgtttttct caccgtttct cggctggctg ggcgtattcc ttaccgggtc
    10441 ggacacttct tcaaacgccc tgtttggttc actgcaatcg accacggcgc agcaaatcaa
    10501 cgtctctgac actctgctgg tggcagcaaa taccagcggc ggcgtaactg gcaagatgat
    10561 ctccccgcaa tctatcgccg tggcctgcgc cgcgacgggc atggtgggcc gagaatctga
    10621 actgttccgc tacaccgtga agcacagtct gatttttgcc agcgttatca ccctgctgca
    10681 ggcgtatgtg tttaccggga tgttagtctc gtaaatcaat aaagccggat gaaggtaaat
    10741 atcacccggc tttccatttg tctgtcgttt atctcgccag ggaaccgttt ttacgattgg
    10801 cttctttata cgtagtctcc gttttacgat gcgaattgtt taatatttat acaacttggc
    10861 ttctgtgctg gttataaagt acccactttc atgagatcaa ttttagtatg ttattacatt
    10921 gcttcatctg ttgctttcat gacgaatggc gttttttctc ttatttatat ttttcactag
    10981 aacaatgagt agatcaaaat tcccaaaact cccaaaaaca taagattatt cgcaaagctg
    11041 attttgcaga ataatgagaa cttttaagat ttgtcgagga tcaattttaa gcaatttatc
    11101 tccacttgtt acataaaaga tgccgttagg ttaataagtt aatcttgcta taacagtgtt
    11161 cgttactgcc tattaataaa gatatttggg atagggtaac gtggatgagg attcgaaatc
    11221 atccgtcttg cg
//
LOCUS       DAZSNO010000123        11215 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZSNO010000123 DAZSNO010000000
VERSION     DAZSNO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11215
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.123"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            8..142
                     /locus_tag="ACHWN2_003052"
     CDS             8..142
                     /locus_tag="ACHWN2_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR8932492.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA
                     "
     gene            121..225
                     /gene="speFL"
                     /locus_tag="ACHWN2_003053"
     CDS             121..225
                     /gene="speFL"
                     /locus_tag="ACHWN2_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR8932493.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            536..2733
                     /gene="speF"
                     /locus_tag="ACHWN2_003054"
                     /pseudo
     CDS             536..2733
                     /gene="speF"
                     /locus_tag="ACHWN2_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2730..4049
                     /gene="potE"
                     /locus_tag="ACHWN2_003055"
     CDS             2730..4049
                     /gene="potE"
                     /locus_tag="ACHWN2_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR8932494.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMAPAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4105..5745)
                     /gene="pgm"
                     /locus_tag="ACHWN2_003056"
     CDS             complement(4105..5745)
                     /gene="pgm"
                     /locus_tag="ACHWN2_003056"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR8932495.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLTDI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5771..6316)
                     /gene="seqA"
                     /locus_tag="ACHWN2_003057"
     CDS             complement(5771..6316)
                     /gene="seqA"
                     /locus_tag="ACHWN2_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR8932496.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6501..7265
                     /gene="ybfF"
                     /locus_tag="ACHWN2_003058"
     CDS             6501..7265
                     /gene="ybfF"
                     /locus_tag="ACHWN2_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR8932497.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7405..7698
                     /gene="ybfE"
                     /locus_tag="ACHWN2_003059"
     CDS             7405..7698
                     /gene="ybfE"
                     /locus_tag="ACHWN2_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR8932498.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7838..8368
                     /gene="fldA"
                     /locus_tag="ACHWN2_003060"
     CDS             7838..8368
                     /gene="fldA"
                     /locus_tag="ACHWN2_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR8932499.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8578..8664
                     /gene="uof"
                     /locus_tag="ACHWN2_003061"
     CDS             8578..8664
                     /gene="uof"
                     /locus_tag="ACHWN2_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR8932500.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8657..9103
                     /gene="fur"
                     /locus_tag="ACHWN2_003062"
     CDS             8657..9103
                     /gene="fur"
                     /locus_tag="ACHWN2_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR8932501.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9187..9513)
                     /gene="chiQ"
                     /locus_tag="ACHWN2_003063"
     CDS             complement(9187..9513)
                     /gene="chiQ"
                     /locus_tag="ACHWN2_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR8932502.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9550..10956)
                     /gene="chiP"
                     /locus_tag="ACHWN2_003064"
     CDS             complement(9550..10956)
                     /gene="chiP"
                     /locus_tag="ACHWN2_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFR8932503.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2730 a         2766 c         2797 g         2922 t
ORIGIN      
        1 ataattaatg atttattatc tggggtatga tcgttttttg ttgatcttct tcacagatta
       61 tagccatttc atggatagaa taactctacc ttcaactgac acagcaagcg gtaaaggtaa
      121 atggaaaata ataaccgctt aatgcctcat ataaggcgga caacccatat catgaagttt
      181 gcccatcgta atagttttga ctttcatttc tttaatgccc gctagtcttc tgactaaagg
      241 gcaccccaac gtacaggtct ccctgacttt aagcattaca ggttaatacc tgtattcctc
      301 ggtgctcata tactgctaac ccttttaaac tctaaataat tcgagtcgca gcacttgcaa
      361 cttgaggtat gacgagtata gccagttacc gggctggtct gggttattgc atctgcaaaa
      421 agcaaactac tgatttattt atcagcggtg gagctttgct ttttttccga cgtgatcgat
      481 ttctcctttg agaaattgag gacctgctat tacctgaaat aaagagatga ataaaatgtc
      541 agaattaaaa attgccgtta gccgttcttg cccggattgt ttttccactc atcgtgcatg
      601 cgtgaatatt gacgaaagta attatattga cgttgccgcc attattttat cagtcaatga
      661 tgttgaacgt ggaaaactcg ataaaataga cgctactggc tatggcattc ctgtttttat
      721 tgcaacggaa aatgaagaac gtgtaccagc agaatatctg ccacgtattt ctggtgtctt
      781 cgagcattgc gaatcacgga aagagtttta tggacgtcag ttagaaaccg ctgctagcca
      841 ttatgaaacg caactgcgcc cgcccttctt ccgcgcactg gtcgattatg tcaatcaggg
      901 caatagcgct tttgactgcc cgggtcatca gggaggtgag tttttccgtc gtcatccggc
      961 agggaatcag tccgtggagt acttcggaga aacgctgttc cgttccgact tatgcaacgc
     1021 agacgtagca atgggcgatc tgctgattca cgaaggcgcg ccatgcattg cacagcaaca
     1081 tgcggcaaaa gtgtttaatg ccgataaaac atacttcgtt ctaaatggca cttcatcttc
     1141 taacaaagtg gttttaaacg ccctgctaac accgggtgat ctggtgctgt ttgatcgcaa
     1201 taaccacaaa tctaaccacc acggagcgtt gctacaggct ggtgcaacac cggtttatct
     1261 ggaaacggca cgtaacccgt atggctttat cggtggcatt gatgcgcact gttttgaaga
     1321 aagttacctg cgtgagctga tcgcggaagt cgcaccgcag cgggcaaaag aggctcgtcc
     1381 tttccgcctc gctgtgattc agttaggcac ctacgacggt acgatttata acgcccgcca
     1441 ggtggtggat aaaattggtc atctgtgtga ctacattctg tttgactccg catgggttgg
     1501 ctatgagcaa tttatcccga tgatggctga ctgttcgccg ctgttgctgg atcttaatga
     1561 gaacgatccg ggtattctag ttacgcaatc cgtacataaa cagcaggctg gtttttctca
     1621 aacttcacaa atccataaaa aagacagcca catcaaaggg caacagcgtt atgtaccgca
     1681 caaacgcatg aacaacgcct ttatgatgca cgcctccacc agcccgttct atccgctgtt
     1741 tgccgcactg gatatcaacg ccaaaatgca tgaaggtgtc agcggtcgta atatgtggat
     1801 ggattgtgtg gtaaatggca ttaatgcccg caaactgatc ctcgataact gtcagcatat
     1861 tcgtccgttc gtacctgaac tggtggatgg taaaccctgg cagtcgtatg aaacagcgca
     1921 aattgcggtt gatctgcgct tcttccagtt tgtaccaggg gaacactggc attcttttga
     1981 aggctatgca gagaatcaat actttgtcga tccttgcaaa ctgttgctga caaccccagg
     2041 tattgatgca cgtaacggcg aatatgaagc gttcggtgta cccgcgacga ttcttgctaa
     2101 cttcctgcgc gaaaatggcg tagtgccgga aaaatgcgat cttaacgcca tcctcttcct
     2161 gctgactccg gcagaagata tggccaaact tcagcaactt gtgccctgct ggtacgcttc
     2221 gaaaaactgc ttgagtccga cgcgccatta gcagaagtgc tgccttccat ctacaaacag
     2281 catgaagagc gctacgccgg ttataccctg cgtcagttgt gccaggaaat gcatgatttg
     2341 tatgcccgcc acaacgtgaa acaactgcaa aaagagatgt tccgcaaagc gcacttccca
     2401 cacgtcagca tgaatccgca agaagccaac tacgcctatt tacgcggtga agtggagctg
     2461 gttcgtctac cggatgccga aggtcgtatc gctgccgaag gggcgcttcc ttatcctccg
     2521 ggtgtgctgt gtgttgttcc gggtgaaatc tggggtggtg ctgttttgcg ttacttcagc
     2581 gctctggaag aagggatcaa cctgctgcca ggttttgcac cggagctgca gggtgtctat
     2641 atcgaagaac atgatggtcg taagcaagtt tggtgctatg tcatcaagcc tcgtgatgcg
     2701 caaagcaccc tgttgaaagg ggaaaaatta tgagtcaggc taaatcgaac aaaatgggcg
     2761 tcgttcagtt aaccatactg acgatggtca acatgatggg ctccggtatc atcatgctgc
     2821 cgacaaagct tgccgaagtt gggacaattt caattatctc ctggctggtg acagccgtag
     2881 gctcaatggc accggcatgg gcattcgcaa aatgcggtat gttcagccgt aaatcaggtg
     2941 gtatgggcgg ttatgccgag tatgctttcg gtaaatccgg taactttatt gcgaactata
     3001 cctacggcgt ctcactgctg attgctaacg tcgcgattgc tatttcggcg gttggttacg
     3061 gcaccgaact gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg accatcgggg
     3121 tgctgtggat ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg caaatcagta
     3181 gcattaccgt gtgggggggc attattccgg tggtaggcct gtgcattatc ggctggttct
     3241 ggtttagccc gacgctttac gttgattcct ggaatccgca tcatgcaccg ttcttcagtg
     3301 cagtaggttc ttccatcgct atgacgctgt gggcttttct tggtctggag tctgcgtgtg
     3361 cgaatactga tgtagtggaa aacccggaac gtaatgtgcc aatcgcggta ctcggcggta
     3421 cgttaggtgc ggcggtgatt tatatcgtct ccaccaacgt gattgcaggg attgtgccta
     3481 atatggagct ggcaaattca acggcaccat ttggcctggc cttcgcacag atgtttacac
     3541 cagaagtggg taaagtcatt atggcgctga tggtgatgtc ctgctgcggt tcgctgcttg
     3601 gctggcagtt caccattgcc caggtgttta aatcttcatc tgatgaaggc tacttcccta
     3661 aaattttctc ccgtgtaacc aaagtggatg caccggtgca gggaatgttg accattgtga
     3721 ttatccagag cgggttggcg ctgatgacga ttagcccgtc gctaaacagt cagttcaacg
     3781 tgctggttaa cctggccgtg gtgaccaata tcattccgta tattctgtca atggcggcat
     3841 tagtcattat tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt gcaaactttg
     3901 ttgcttttgt tggcgcgatg tatagctttt atgcgctgta ctcatccggg gaagaagcca
     3961 tgctgtacgg ttccatcgtg accttccttg gttggacact gtatggtctg gtatcaccac
     4021 gctttgaact gaaaaataaa cacggttaaa aaagcaaagt aaaaaagggc gatcttgcga
     4081 ccgctctttt tttattaaat gtgtttacgc gtttttcaga acttcgctaa caatctcaac
     4141 cgcttctttc tcaatctgct tgcgatgttc ttcaccgagg aagctttcgc agtagatctt
     4201 atatgcgtct tccgtgcctg acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt
     4261 cagaccgcca atagaagcac cgttgcccgg agcagcagtc aggcgcgcgg tgatcgggtc
     4321 acctgccagg gtgctggcgc tcaccatttc cggagacagc ttagacagcg ccgctttttg
     4381 tgcggaagtc gcagctgcct gcaaacggtt gtagctcggc gcaccaaagc gttttgccag
     4441 ttcgttgtag tgttcctgcg ggttcttacc ggtgacagcg gtgatttccg ccgccagcag
     4501 acacataatg atgccgtctt tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga
     4561 agcccctgcg ctctcttcgc cgccaaagcc gaagctgccg tcgaacagac catcaacaaa
     4621 ccatttgaaa cctaccggta cttctaccag cttacggccc aagtcattga ccacacggtc
     4681 gatcatcgca gaagaaacca gcgttttacc gacagcaaca tctttgcccc actgcggacg
     4741 atgctggaac aggtagttga tcgccaccgc caggtagtgg ttcggattca tcaaacctgc
     4801 cggagtgacg ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt
     4861 atcacgcagt gccagcagac ccgccatcgc acactcggag gagcagtcca tacggatcgc
     4921 accgtcttta tcgaggtgca taaagcggaa ggtttgatcg acctgatcgt taacgatggt
     4981 caggttgagg ttgtaatact cgccaatgcg cttccagtat tcgataccgg aaccgcccaa
     5041 cggatcaacg cccagcgtca ggcccgcttt ctgaatcgcc gccatatcaa cgatatcggt
     5101 cagcccttcc acgaacggct gcaccagatc ctgctctttc acatagccgg atgccatcgc
     5161 ttcgtcgagg gagatacgct tcacgccttt caggccatcg gccatcagtg cgttggcctt
     5221 gtcttccacc actttggtga cgttagtatc agccgggcca ccatttggcg gattgtactt
     5281 gataccacca tcttccggcg ggttatggga cggtgtaatc acgataccgt ctgccagcgg
     5341 gccacctttt ttattgtgaa ccaggatggc attggaaacg gcaggcgtcg gagtgaagcc
     5401 attgttttcc tgcacaatga catcaacgcc gttcgctgcc agcacttcca gtacggaaat
     5461 gaacgcaggc tcggacaggg cgtgagtatc tttacccaca tagcaagggc cagtgatgcc
     5521 gtttttcgca cgttcttcag caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa
     5581 gctgtggcgc gctgcactgc cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc
     5641 attccctgct tctggtttca gtacataata ttgcgccgtc agttgggcga cgttaatcaa
     5701 atcactctgt tgtgcaggtt ggcctgcacg attgtggatt gccattgctt tgtcctttgt
     5761 ctgcaacgtt ttagatagtt ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg
     5821 actgcatgat gtgttcgatc atgctgcatt tacggccggt gttggtgttg gtgatcaccc
     5881 aatacggcgt gcctggcaca tgtttcggct tggtctgatt accatttttc agcagcgttt
     5941 gttcatctgc cgcaaagtac acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa
     6001 acgcctgggc gtcaagagaa tatagtgtag acaacagcag cataaagcga ttgaccgctc
     6061 gcttttgctc tgcgtattcg tccgaaagca gaagttcacg cattgcgcga accttgtctt
     6121 taatcgtttt gaccggcttc gcttcgacga tagcaggtga cgcaacgcga acctctttcg
     6181 tcaccggagc agcaggctgt gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg
     6241 atgcgctctc gccgatatgc ttagtgtggc tggcaatata gctgtagagt tcatcatcaa
     6301 cttcaatcgt tttcatctta atccagtgca gtgtcttagc tgaatacaag tcgccaagag
     6361 tatatgaata aatcccggca gcggatagcg tcaagcctgg cttgcagaaa aactcggaat
     6421 aaaccttatt tgttgcagcc ctgtggcaca atggtcaaca taatacccta acccgacaac
     6481 gcgaaaaaaa gaactttgcc atgaaattaa atatccgcgc gcaaactgca caaaaccagc
     6541 acaataattc tcccatcgtt cttgtccatg gtctgtttgg tagcctcgac aaccttggcg
     6601 tactggctcg cgatctggta aacgatcaca atatcatcca ggttgatatg cgtaaccacg
     6661 gtctttcacc gagagagccg gtaatgaatt acccggcgat ggcgcaggat cttgttgata
     6721 ccctggatgc actgcagatc gacaaagcaa catttatcgg tcactccatg ggcggtaaag
     6781 cggtaatggc acttactgca ctggcccccg atcgcatcga taaactggtg gcgatcgata
     6841 tcgcaccggt cgactatcac gtacgccgtc atgatgagat tttcgcagct atcaacgcgg
     6901 tcagtgaatc ggacgcacaa actcgccagc aagcagcagc aataatgcgc cagcatctta
     6961 atgaagaagg ggtgattcag tttctgttga aatcttttgt tgacggggag tggcgcttta
     7021 acgtgccggt attgtgggat cagtatccgc atattgtggg ttgggagaaa atcccggcat
     7081 gggatcaccc tgccctgttt atccctggcg gcaattctcc gtatgttagc gagcagtacc
     7141 gtgatgattt actggcacaa tttccacagg cacgggcgca tgtgattgca ggcgcgggtc
     7201 actgggtcca tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg
     7261 attaacgcaa ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg
     7321 tgactcatcg cgctgatgta ttatggcgcg ctatccatcc gggcagaggc ctggctgatt
     7381 gtttcccccg aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac gacattagat
     7441 ctgttcgcgc acgagcgtcg accgggacga ccgaaaacta atccgctttc gcgcgatgaa
     7501 cagctgcgta ttaataaacg caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt
     7561 gtcgaactga agctgaacgc ggaagctgtc gaggcgctga acgagctggc ggaatcgcgt
     7621 aatatgagcc gtagcgaact gatcgaagag atgttaatgc agcaactggc ggcgctgcgt
     7681 agtcagggca tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt
     7741 gctgattccg caagactgcc tgttctgcta tgattgcctt tatccgtggg caattttcca
     7801 cccccatttc aataagtttc aagaggttat ttcactcatg gctatcactg gcatcttttt
     7861 cggcagcgac accggtaata ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa
     7921 agacgttgcc gatgtccatg acattgcaaa aagcagcaaa gaagatctgg aagcttacga
     7981 cattctgctg ctgggcatcc caacctggta ttacggcgaa gcgcagtgtg actgggatga
     8041 cttcttcccg actctcgaag agattgattt caatggtaaa ctggttgcgc tgtttggttg
     8101 tggtgaccag gaagattacg ccgaatattt ctgcgacgca ttgggcacca tccgcgacat
     8161 cattgaaccg cgcggtgcaa ccatcgttgg tcactggcca actgcgggct atcatttcga
     8221 agcatcaaaa ggtctggcag atgacgacca ctttgtcggt ctggctatcg acgaagaccg
     8281 tcagccggaa ctgaccgctg aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt
     8341 gcatctcgac gaaattctta atgcctgatg tgatgcggcg tagactcatg tctacgccgt
     8401 attaatagat aatgccaatc aaaataattg ctacaaattt gtaacttttg ctgttgtacc
     8461 tatacaatgt cccagtgttc aagtggcctt gccgttgtaa atgtaagctg tgccacgttt
     8521 ttattaacaa tatttgccag ggacttgtgg ttttcattta ggcgtggcaa ttctataatg
     8581 atacgcatta tctcaagagc aaattctgtc acttcttcta atgaagtgaa ccgcttagta
     8641 acaggacaga ttccgcatga ctgataacaa taccgcccta aagaaagctg gcctgaaagt
     8701 aacgcttcct cgtttaaaaa tcctggaagt tcttcaggag ccggacaacc atcacgtcag
     8761 tgcggaagat ttatacaaac gtctgatcga tatgggtgaa gaaattggtc tggctacggt
     8821 atatcgcgta ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca attttgaagg
     8881 cggtaaatcc gtatttgaac tgacacagca acatcaccac gatcacctga tttgcctcga
     8941 ctgcggcaag gttatcgaat ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc
     9001 cgcaaaacat ggcattcgcc tgactaacca cagtctctat ctttacggtc actgtgccga
     9061 aggcgattgc cgcgaagatg agcacgcgca cgaaggcaaa taagccagcc tgaacgagaa
     9121 aagccaacct gcgggttggc ttttttatac aagggaaata agaaaagcga gagttacagc
     9181 tctcacttat ttgttattac tgcgtatttc ctgccagacc ttatcacaat cggctttcac
     9241 cgcctgatca tttccggttt gcgttgcacg cagacactgc tgataatcca gtacgcgtac
     9301 actttcctgg tcggcaaact gctgatgttg tttctctttc ttcagcacgt ttaacacgct
     9361 ctggcaggct tcaatttttt ctggcgaacc ttgcgcggtg ttgatacagg cgctgtatgc
     9421 ctctttcaga cggctgtctt cctgtggtgc ggttgattgc gcacaagcta ccagccccga
     9481 tgccatcatg gcgatgagaa tcagtttttt cattgaatgc ggcaggcaca aaacctgccg
     9541 cgtcgggcat cagaagatgg tgaatggtgc gattaccata aatttcacgt cacgctcatc
     9601 ctggaagatg ttaccgtaac cgccgcccca gcttgggatg tcggagtggt tgtcgtattc
     9661 ggtgaagtgc agtttgaaca tcgtgccttt ggcgcgaccg tcctgaatgg tgtagaccgc
     9721 atccaggctg taggcagact cttcaatagt ccggtttttg tcgtagtacg catccggatt
     9781 gctctgccag gtcgcaggtt tagcatccca tgcgtaaacg taggaagcgc cgatggcgaa
     9841 gcctggaaga ttccagtttt tcaggtcata catcgcactg aagaagaccg ctttttcgcc
     9901 gttagcgttg aagtcggaac ggttatccca ccagatatcc aggcggccgt tggaggaagc
     9961 gtaggttggg gtcatacgtt gcaggaagta tccctgctga ctgtcagcct taatccaggt
    10021 gccttcgagg cgcaaatcca ctacgtcagc cgcccggtaa ccaaaggtca acgcttgcag
    10081 ccaggcggtg ccgtcataaa gatcgttgac gctgcgatcg tcaactttat cgcgggtacc
    10141 atagaactgg tagctggtgg ttaacgggct accggcgata tcaaatttgt agctggcttt
    10201 ggcaaaatat tgatcgatat acccttccgc ctgaccaaat gccgcttcca gtacgaagtt
    10261 atttttgaag tcgtatttcg caccgatgga gtgcagataa tcaactttgg tggttttatc
    10321 gttctgataa aactcatcca tttccagatg ccacggcgct ttgtattcgt tggtccacat
    10381 gtaggagaaa ctcaaggcac cagcatcgcc gtaatcaaaa ttcgccccgg cttccgcccc
    10441 ctgataagta cccggcataa agctccagtg cggcgctaac agcgtttgac cggttggctg
    10501 aatgtaacct gccctcgccc aaaccggacc gtatttaaat ttggccgcag ctttatacag
    10561 gcttataccg cttttgtcgc cggaccagtc ttcgtcatag gctttattac tttttgaaaa
    10621 cgcgatttcg ttcgggtggg agctgtcgcc gttttccgcc atttcaatcg ccgtaaacgc
    10681 agcaatatca aggccgaaca tatcagcagc ataaccggac tgaaaatcga ggttggcatt
    10741 ccaggtggag tgagaaaggt tggttttgta tttgtcgccg tcggtaacat ctttacggtc
    10801 gcgttcacgc tgccagtaat agataccgcc ggttaaggtt gaatcgtcga tgaatccttc
    10861 ggcgcgagcc tccggcattg ccataaagcc cgacattgct gtaacaccgg caatagccag
    10921 cgccagcgta ctacgtttgc cactaaacgt acgcatgggt taatcctctt tgacgtataa
    10981 attgctgcac caaaggtgaa cagcgaaaaa aaataaaaat taaaaaagta gcgttgagaa
    11041 aagtctcgct gactacagga acagactatt tttcgcgacg cgaattatca atctctttct
    11101 gtaaccagaa tgtaagaaca tgagcaaccg cacatatttt gatgattttt gttacaaggc
    11161 ttaagtttgc ggttgaaagg cttgcagaag aataaaaacg tgaaattcca gcaag
//
LOCUS       DAZSNO010000124        11098 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZSNO010000124 DAZSNO010000000
VERSION     DAZSNO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11098
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.124"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            470..850
                     /gene="atpI"
                     /locus_tag="ACHWN2_003065"
     CDS             470..850
                     /gene="atpI"
                     /locus_tag="ACHWN2_003065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR8932504.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            859..1674
                     /gene="atpB"
                     /locus_tag="ACHWN2_003066"
     CDS             859..1674
                     /gene="atpB"
                     /locus_tag="ACHWN2_003066"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR8932505.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            1721..1960
                     /gene="atpE"
                     /locus_tag="ACHWN2_003067"
     CDS             1721..1960
                     /gene="atpE"
                     /locus_tag="ACHWN2_003067"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR8932506.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            2022..2492
                     /gene="atpF"
                     /locus_tag="ACHWN2_003068"
     CDS             2022..2492
                     /gene="atpF"
                     /locus_tag="ACHWN2_003068"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR8932507.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            2507..3040
                     /gene="atpH"
                     /locus_tag="ACHWN2_003069"
     CDS             2507..3040
                     /gene="atpH"
                     /locus_tag="ACHWN2_003069"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR8932508.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            3053..4594
                     /gene="atpA"
                     /locus_tag="ACHWN2_003070"
     CDS             3053..4594
                     /gene="atpA"
                     /locus_tag="ACHWN2_003070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR8932509.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            4645..5508
                     /gene="atpG"
                     /locus_tag="ACHWN2_003071"
     CDS             4645..5508
                     /gene="atpG"
                     /locus_tag="ACHWN2_003071"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR8932510.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            5535..6917
                     /gene="atpD"
                     /locus_tag="ACHWN2_003072"
     CDS             5535..6917
                     /gene="atpD"
                     /locus_tag="ACHWN2_003072"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR8932511.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            6938..7357
                     /locus_tag="ACHWN2_003073"
     CDS             6938..7357
                     /locus_tag="ACHWN2_003073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR8932512.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            7708..9078
                     /gene="glmU"
                     /locus_tag="ACHWN2_003074"
     CDS             7708..9078
                     /gene="glmU"
                     /locus_tag="ACHWN2_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR8932513.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            9240..11069
                     /gene="glmS"
                     /locus_tag="ACHWN2_003075"
     CDS             9240..11069
                     /gene="glmS"
                     /locus_tag="ACHWN2_003075"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR8932514.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         2681 a         2743 c         3023 g         2651 t
ORIGIN      
        1 agtcattagt gaaaatatca gtctgctaaa aatcggcgct aagaaccatc attggctgtt
       61 aaaacattat taaaaatgtc aatgagtggt ttttgttgtg taaatgttat ttattaaaac
      121 agtatctgtt tttagactga aatatcataa acttgtaaag gcatcatttg ccaagtaaat
      181 aaatatgctg tgcgcgaaca tgcgcaatat gtgatctgaa gcacgcttta tcaccagtgt
      241 ttacgcgtta tttacagttt ttcatgatcg aacaggggta gcggaaaagt cgcaattgta
      301 tgcactggaa aaatatttaa acatttattc accttttggc tacttattgt ttgaaatcac
      361 gggggcgcac cgtataattt gaccgctttt tgatgcttga ctctaagcct taaagaaagt
      421 tttatacgac acgcggcata cctcgaaggg agcaggagtg aaaaacgtga tgtctgtgtc
      481 gctcgtgagt cgaaacgttg ctcggaagct tctgctcgtt cagttactgg tggtgatagc
      541 aagtggattg ctgttcagcc tcaaagaccc cttctggggc gtctctgcaa taagcggggg
      601 cctggcagtc tttctgccta acgttttgtt tatgatattt gcctggcgtc accaggcgca
      661 tacaccagcg aaaggccggg tggcttggac attcgcattt ggcgaagctt tcaaagttct
      721 ggcgatgttg gtgttactgg tggtggcgtt ggcggtttta aaggcggtat tcttgccgct
      781 gatcgttacg tgggttttgg tgctggtggt tcagatactg gcaccggctg taattaacaa
      841 caaagggtaa aaggcatcat ggcttcagaa aatatgacgc cgcaggatta cataggacac
      901 cacctgaata accttcagct ggacctgcgt acattctcgc tggtggatcc acaaaacccc
      961 ccagccacct tctggacaat caatattgac tccatgttct tctcggtggt gctgggtctg
     1021 ttgttcctgg ttttattccg tagcgtagcc aaaaaggcga ccagcggtgt gccaggtaag
     1081 tttcagaccg cgattgagct ggtgatcggc tttgttaatg gtagcgtgaa agacatgtac
     1141 catggcaaaa gcaagctgat tgctccgctg gccctgacga tcttcgtctg ggtattcctg
     1201 atgaatctga tggatttact gcctatcgac ttgctgccgt acattgctga acatgtactg
     1261 ggtctgcctg cactgcgtgt ggttccgtct gcggacgtga acgtaacgct gtctatggca
     1321 ctgggcgtat ttatcctgat tctgttctac aacatcaaaa tgaaaggcat cggcggcttc
     1381 acgaaagagt tgacgctgca gccgttcaat cactgggcgt tcattcctgt caacttaatc
     1441 cttgaagggg taagcctgct gtccaaacca gtttcactcg gtttgcgact gttcggtaac
     1501 atgtatgccg gtgagctgat tttcattctg attgctggtc tgttgccgtg gtggtcacag
     1561 tggatcctga atgtgccgtg ggccattttc cacatcctga tcattacgct gcaagccttc
     1621 atcttcatgg ttctgacgat cgtctatctg tcgatggcgt ctgaagaaca ttaatttacc
     1681 aacactacta cgttttaact gaaacaaact ggagactgtc atggaaaacc tgaatatgga
     1741 tctgctgtac atggctgccg ctgtgatgat gggtctggcg gcaatcggtg ctgcgatcgg
     1801 tatcggcatc ctcgggggta aattcctgga aggcgcagcg cgtcaacctg atctgattcc
     1861 tctgctgcgt actcagttct ttatcgttat gggtctggtg gatgctatcc cgatgatcgc
     1921 tgtaggtctg ggtctgtacg tgatgttcgc tgtcgcgtag taagcgttgc ttttatttaa
     1981 agagcaatat cagaacgtta actaaataga ggcattgtgc tgtgaatctt aacgcaacaa
     2041 tcctcggcca ggccatcgcg tttgtcctgt tcgttctgtt ctgcatgaag tacgtatggc
     2101 cgccattaat ggcagccatc gaaaaacgtc aaaaagaaat tgctgacggc cttgcttccg
     2161 cagaacgagc acataaggac cttgaccttg caaaggccag cgcgaccgac cagctgaaaa
     2221 aagcgaaagc ggaagcccag gtaatcatcg agcaggcgaa caaacgccgc tcgcagattc
     2281 tggacgaagc gaaagctgag gcagaacagg aacgtactaa aatcgtggcc caggcgcagg
     2341 cagaaattga agccgagcgt aaacgtgccc gtgaagagct gcgtaagcaa gttgctatcc
     2401 tggctgttgc tggcgccgag aagatcatcg aacgttccgt ggatgaagct gctaacagcg
     2461 acatcgtgga taaacttgtc gctgaactgt aaggagggag gggctgatgt ctgaatttat
     2521 tacggtagct cgcccctacg ccaaagcagc ttttgacttt gccgtcgaac accaaagtgt
     2581 agaacgctgg caggacatgc tgacgtttgc cgccgaggta accaaaaacg aacaaatggc
     2641 agagcttctc tctggcgcgc ttgcgccaga aacgctcgcc gagtcgttta tcgcagtttg
     2701 tggtgagcaa ctggacgaaa acggtcagaa cctgattcgg gtaatggctg aaaatggtcg
     2761 tcttaacgcg ctcccggatg ttctggagca gtttattcac cttcgtgccg tgagtgaggc
     2821 taccgctgag gtagacgtca tttccgctgc cgcactgagt gaacaacagc tcgcgaaaat
     2881 ttctgctgcg atggaaaaac gtctgtcacg caaagttaag ctgaattgca aaatcgataa
     2941 gtctgtaatg gctggcgtta tcatccgatc gggtgatatg gtcattgatg gcagcgtacg
     3001 cggtcgtctt gagcgccttg cagacgtctt gcagtcttaa ggggactgga gcatgcaact
     3061 gaattccacc gaaatcagcg aactgatcaa gcagcgcatt gctcagttca atgttgtgag
     3121 tgaagctcac aacgaaggta ctattgtttc tgtaagtgac ggtgttatcc gcattcacgg
     3181 cctggccgat tgtatgcagg gtgaaatgat ctccctgccg ggtaaccgtt acgctatcgc
     3241 actgaacctc gagcgcgact ctgtaggtgc ggttgttatg ggtccgtacg ctgaccttgc
     3301 cgaaggcatg aaagttaagt gtactggccg tatcctggaa gttccggttg gccgtggcct
     3361 gctgggccgt gtggttaaca ctctgggtgc accaatcgac ggtaaaggtc cgctggatca
     3421 cgacggcttc tctgctgtag aagcaatcgc tccgggcgtt atcgaacgtc agtccgtaga
     3481 tcagccggta cagaccggtt ataaagccgt tgactccatg atcccaatcg gtcgtggtca
     3541 gcgtgaattg atcatcggtg accgtcagac aggtaaaacc gcactggcta tcgatgccat
     3601 catcaaccag cgcgattccg gtatcaaatg tatctatgtc gctatcggcc agaaagcgtc
     3661 caccatttct aacgtggtac gtaaactgga agagcacggc gcactggcta acaccatcgt
     3721 tgtggtagca accgcgtctg aatccgctgc actgcaatac ctggcaccgt atgccggttg
     3781 cgcaatgggc gaatacttcc gtgaccgcgg tgaagatgcg ctgatcattt acgatgacct
     3841 gtctaaacag gctgttgctt accgtcagat ctccctgctg ctccgtcgtc cgccaggacg
     3901 tgaagcattc ccgggcgacg ttttctacct ccactctcgt ctgctggagc gtgctgcacg
     3961 tgttaacgcc gaatacgttg aagccttcac caaaggtgaa gtgaaaggga aaaccggttc
     4021 tctgaccgca ctgccgatta tcgaaactca ggcgggtgac gtttctgcgt tcgttccgac
     4081 caacgtaatc tccattaccg atggtcagat cttcctggaa accaacctgt tcaacgccgg
     4141 tattcgtcct gcggttaacc cgggtatttc cgtatcccgt gttggtggtg cagcacagac
     4201 caagatcatg aaaaaactgt ccggtggtat ccgtaccgct ctggcacagt atcgtgaact
     4261 ggcagcgttc tctcagtttg catccgacct tgacgatgca acacgtaagc agcttgacca
     4321 cggtcagaaa gtgaccgaac tgctgaaaca gaaacagtat gcgccgatgt ccgttgcgca
     4381 gcagtctctg gttctgttcg cagcagaacg tggttacctg gcggatgttg aactgtcgaa
     4441 aattggcagc ttcgaagccg ctctgctggc ttacgtcgac cgtgatcacg ctccgttgat
     4501 gcaagagatc aaccagaccg gtggctacaa cgacgaaatc gaaggcaagc tgaaaggcat
     4561 cctcgattcc ttcaaagcaa cccaatcctg gtaacgtctg gaggcttgcc ttagggcagg
     4621 ccgcaaggca ttgaggagaa gctcatggcc ggcgcaaaag agatacgtag taagatcgca
     4681 agcgtccaga acacgcaaaa gatcactaaa gcgatggaga tggtcgccgc ttccaaaatg
     4741 cgtaaatcgc aggatcgcat ggcggccagc cgtccttatg cagaaaccat gcgcaaagtg
     4801 attggtcacc ttgcacacgg taatctggaa tataagcacc cttacctgga agaccgcgac
     4861 gttaaacgcg tgggctacct ggtggtgtcg accgaccgtg gtttgtgcgg tggtttgaac
     4921 attaacctgt tcaaaaaact gctggcggaa atgaagacct ggaccgacaa aggcgttcaa
     4981 tgcgacctcg caatgatcgg ctcgaaaggc gtgtcgttct tcaactccgt gggcggcaat
     5041 gttgttgccc aggtcaccgg catgggggat aacccttccc tgtccgaact gatcggtccg
     5101 gtaaaagtga tgttgcaggc ctacgacgaa ggccgtctgg acaagcttta cattgtcagc
     5161 aacaaattta ttaacaccat gtctcaggtt ccgaccatca gccagctgct gccgttaccg
     5221 gcatcagatg atgatgatct gaaacataaa tcctgggatt acctgtacga acccgatccg
     5281 aaggcgttgc tggataccct gctgcgtcgt tatgtcgaat ctcaggttta tcagggcgtg
     5341 gttgaaaacc tggccagcga gcaggccgcc cgtatggtgg cgatgaaagc cgcgaccgac
     5401 aatggcggca gcctgattaa agagctgcag ttggtataca acaaagctcg tcaggccagc
     5461 attactcagg aactcaccga gatcgtctcg ggggccgccg cggtttaaac aggttatttc
     5521 gtagaggatt taagatggct actggaaaga ttgtccaggt aatcggcgcc gtagttgacg
     5581 tcgaattccc tcaggatgcc gtaccgcgcg tgtacgatgc tcttgaggtg caaaatggta
     5641 atgagcgtct ggtgctggaa gttcagcagc agctcggcgg cggtatcgtg cgtaccatcg
     5701 caatgggttc ctccgacggt ctgcgtcgcg gtctggatgt aaaagacctc gaacacccga
     5761 tcgaagtccc ggtaggtaaa gcgactctgg gccgtatcat gaacgtactg ggtgaaccgg
     5821 tcgacatgaa aggcgagatc ggtgaagaag agcgttgggc gattcaccgc gcagcacctt
     5881 cctacgaaga gctgtcaaac tctcaggaac tgctggaaac cggtatcaaa gttatcgacc
     5941 tgatgtgtcc gttcgctaag ggcggtaaag ttggtctgtt cggtggtgcg ggtgtaggta
     6001 aaaccgtaaa catgatggag ctcattcgta acatcgcgat cgagcactcc ggttactctg
     6061 tgtttgcggg cgtaggtgaa cgtactcgtg agggtaacga cttctaccac gaaatgaccg
     6121 actccaacgt tatcgacaaa gtatccctgg tgtatggcca gatgaacgag ccgccgggaa
     6181 accgtctgcg cgttgctctg accggtctga ccatggctga gaaattccgt gacgaaggtc
     6241 gtgacgttct gctgttcgtt gacaacatct atcgttacac cctggccggt acggaagtat
     6301 ccgcactgct gggccgtatg ccttcagcgg taggttatca gccgaccctg gcggaagaga
     6361 tgggcgttct gcaggaacgt atcacctcca ccaaaactgg ttctatcacc tccgtacagg
     6421 cggtatacgt acctgcggat gacttgactg acccgtctcc ggcaaccacc tttgcgcacc
     6481 ttgacgcaac cgtggtactg agccgtcaga tcgcgtctct gggtatctac ccggccgttg
     6541 acccgctgga ctccaccagc cgtcagctgg acccgctggt ggttggtcag gaacactacg
     6601 acaccgcgcg tggcgttcag tccatcctgc aacgttatca ggaactgaaa gacatcatcg
     6661 ccatcctggg tatggatgaa ctgtctgaag aagacaaact ggtggtagcg cgtgctcgta
     6721 agatccagcg cttcctgtcc cagccgttct tcgtggcaga agtattcacc ggttctccgg
     6781 gtaaatacgt ctccctgaaa gacaccatcc gtggctttaa aggcatcatg gaaggcgaat
     6841 acgatcacct gccggagcag gcgttctaca tggtcggttc catcgaagaa gctgtggaaa
     6901 aagccaaaaa actttaacgc cttaatcgga gggtgatatg gcaatgactt accacctgga
     6961 cgtcgtcagc gcagagcaac aaatgttctc tggtctggtc gagaaaatcc aggtaacggg
     7021 tagcgaaggt gaactgggga tctaccctgg ccacgcaccg ctgctcaccg ccattaagcc
     7081 tggtatgatt cgcatcgtga aacagcacgg tcacgaagag tttatctatc tgtctggcgg
     7141 cattcttgaa gtgcagcctg gcaacgtgac cgttctggcc gacaccgcaa ttcgcggcca
     7201 ggatctcgac gaagcgcgag ccatggaagc gaaacgtaag gctgaagagc acattagcag
     7261 ctctcacggc gacgtagatt acgctcaggc gtctgcggaa ctggccaaag cgatcgcgca
     7321 gctgcgcgtt atcgagttga ccaaaaaagc gatgtaacac cggcttgaaa agcacaaaag
     7381 ccagtctgga aacaggctgg ctttttttac gcgttgaccc gtcctgaaga gcgttcacat
     7441 agatcctgct gatataaaac acccctgttt tcccgtttat tcattgatcg aaataagagc
     7501 aaaaacatcc acctgacgct taaatgaagg tactgcctta attttctgca gataaaaggc
     7561 gtgacgatgg tcgaaaatgg cactttcgtc agcggggata atccgttatt gaacaattta
     7621 tcctctgtcc atttcacgat gaaaaaaatg tagttttttc aaggtgaagc ggtttaaatt
     7681 cgttctcaaa ttacagtcag gacgcgtatg ttgaataatg ctatgagcgt agtgatcctt
     7741 gccgcaggca aaggcacgcg catgtattcc gatcttccga aagtgctgca tacccttgcc
     7801 gggaaagcga tggttcagca tgtcattgat gctgcgaatg aattaggcgc agcgcacgtt
     7861 cacctggtgt acggtcacgg cggcgatctg cttaaacagg cgctgaaaga cgacaacctt
     7921 aactgggtgc ttcaggcaga gcagctgggt acgggtcatg caatgcagca ggccgcacct
     7981 ttctttgccg atgatgaaga cattttaatg ctctacggcg acgtgccgct gatctctgtc
     8041 gaaacactcc agcgcctgcg tgatgctaaa ccccagggtg gcattggtct gctgacggtg
     8101 aaactggatg atccgaccgg ttatggacgt atcacccgtg aaaatggcaa agttaccggc
     8161 attgttgagc acaaagatgc caccgacgag cagcgtcaga ttcaggagat caacaccggc
     8221 attctgattg ccaacggcgc agatatgaaa cgctggctgg cgaagctgac caacaataat
     8281 gctcagggcg aatactacat caccgacatt attgcgctgg cgtatcagga agggcgtgaa
     8341 atcgtcgccg ttcatccgca acgtttaagc gaagtagaag gtgtgaataa ccgcctgcaa
     8401 ctctcccgtc tggagcgcgt ttaccagtcc gaacaggctg aaaaactgct gttagcaggc
     8461 gttatgctgc gcgatccggc gcgttttgat ctgcgcggta cgcttactca cgggcgcgat
     8521 gttgaaattg atactaacgt tatcatcgag ggcaacgtga ctctcggtca tcgcgtgaaa
     8581 atcggcaccg gttgcgtgat taaaaacagc gtgattggcg atgattgcga aatcagtccg
     8641 tataccgttg tggaagacgc gaatctggca gcggcctgta ctattggccc gtttgcccgt
     8701 ctgcgtcctg gtgctgagtt gctggaaggt gcacacgttg gtaacttcgt tgagatgaaa
     8761 aaagcgcgtc tgggtaaagg cacgaaagcc gggcatctga cttacctggg cgatgcggaa
     8821 attggcgata acgttaacat cggcgcggga accattacct gcaactacga tggtgcgaat
     8881 aaatttaaga ccattatcgg cgacgatgtg tttgtcggtt ccgacactca gctggtggcc
     8941 ccggtaacag taggcaaagg cgcgaccatt gctgcgggta caactgtgac gcgtaatgtc
     9001 ggcgaaaatg cattagctat cagccgtgtg ccgcagactc agaaagaagg ctggcgtcgt
     9061 ccggtaaaga aaaagtaatt ctggccgggt aatccggtca tctgggatga ggagataaca
     9121 taatctccct cccacaagca gtaactataa aaataacccc actctctaca aggctcgggg
     9181 cgcccgaaaa aacgggcata caggttgacc gacaacgata taaatcggaa tcaaaaacta
     9241 tgtgtggaat tgttggcgcg atcgcgcaac gtgatgtagc agaaatcctt cttgaaggtt
     9301 tacgtcgtct ggaataccgc ggatatgact ctgccggtct ggccgttgtt gatgcggaag
     9361 gtcatatgac tcgcctgcgt cgcctcggta aagtccagat gctggctcag gcagcggaag
     9421 aacatcctct gcatggcggc accggtattg ctcacactcg ctgggcgacc cacggtgaac
     9481 cttcagaagc taacgcgcat ccgcatgttt ctgaacacat tgtggtggtg cataatggca
     9541 tcatcgaaaa ccatgaaccg ctgcgtgaag agttaaaagc gcgtggctat accttcgttt
     9601 ctgaaaccga caccgaagtg attgcccatc tggtgaactg ggagctgaaa caaggcggga
     9661 ctctgcgtga ggctgttctg cgtgctatcc cgcagctgcg cggtgcgtac ggtacagtga
     9721 tcatggactc ccgtcacccg gataccctgc tagcggcacg ttctggtagt ccgctggtga
     9781 ttggcctggg tatgggcgaa aactttatcg cttctgacca gctggcgctg ttgccggtga
     9841 cccgtcgctt tatcttcctt gaagagggcg atattgcgga aatcactcgc cgttcggtaa
     9901 acatcttcga taaaactggc gcggaagtaa aacgtcagga tatcgaatcc aatctgcaat
     9961 atgacgcggg cgataaaggt atttaccgtc actacatgca gaaagagatc tacgaacagc
    10021 cgaacgcgat aaaaaacacc cttaccgggc gcatcagcca cggtcaggtt gatttaagcg
    10081 agctgggacc gaacgccgac gaactgctgt cgaaggttga gcatattcag atcctcgcct
    10141 gtggtacttc ttataactcc ggtatggttt cccgctactg gtttgaatcg ctggcaggta
    10201 ttccgtgcga cgtcgaaatc gcctctgaat tccgctatcg taaatctgcc gtgcgtcgta
    10261 acagcctgat gatcaccttg tcacagtctg gcgaaaccgc ggataccctg gcgggcctgc
    10321 gtctgtcgaa agagctgggt taccttggtt cgctggcaat ctgtaacgtt ccgggttctt
    10381 ctctggtgcg cgaatccgat ctggcgctga tgaccaacgc gggtacggaa atcggcgtgg
    10441 cgtccactaa agcattcacc actcagttaa ctgtgctgtt gatgctggtg gcgaagctgt
    10501 ctcgcctgaa aggtctggat gcctccattg aacatgacat tgtgcatggt ctgcaggcgc
    10561 tgccgagccg tattgagcag atgctgtctc aggataaacg cattgaagct ctggcagaag
    10621 atttctctga caaacatcac gcgctgttcc tgggccgtgg cgatcagtac ccaatcgcgc
    10681 tggaaggcgc attgaagctg aaagagatct cttacattca cgctgaagcc tacgctgcag
    10741 gcgagctgaa acacggcccg ctggcgctga ttgacgcgga tatgccggtt atcgtcgttg
    10801 caccgaacaa cgaactgttg gaaaaactga aatccaacat tgaagaagtt cgcgcgcgtg
    10861 gcggtcaatt gtatgtcttc gccgatcagg atgcgggttt tgtaagtagc gataacatgc
    10921 acatcatcga gatgccgcat gtggaagagg tgattgcacc gatcttctac accgttccgc
    10981 tgcagctgct ggcttaccat gtcgcgctga tcaaaggcac cgacgttgac cagccgcgta
    11041 acctggcaaa atcggttacg gttgagtaat cactatcata gataaaaaag ggctgccg
//
LOCUS       DAZSNO010000125        11016 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZSNO010000125 DAZSNO010000000
VERSION     DAZSNO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.125"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            110..1009
                     /gene="lysO"
                     /locus_tag="ACHWN2_003076"
     CDS             110..1009
                     /gene="lysO"
                     /locus_tag="ACHWN2_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR8932515.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1153..2805
                     /gene="hcp"
                     /locus_tag="ACHWN2_003077"
                     /gene_synonym="priS"
     CDS             1153..2805
                     /gene="hcp"
                     /locus_tag="ACHWN2_003077"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR8932516.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            2817..3785
                     /gene="hcr"
                     /locus_tag="ACHWN2_003078"
     CDS             2817..3785
                     /gene="hcr"
                     /locus_tag="ACHWN2_003078"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR8932517.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3918..5636
                     /gene="poxB"
                     /locus_tag="ACHWN2_003079"
     CDS             3918..5636
                     /gene="poxB"
                     /locus_tag="ACHWN2_003079"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR8932518.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5673..6674
                     /gene="ltaE"
                     /locus_tag="ACHWN2_003080"
     CDS             5673..6674
                     /gene="ltaE"
                     /locus_tag="ACHWN2_003080"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR8932519.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6685..8115
                     /locus_tag="ACHWN2_003081"
     CDS             6685..8115
                     /locus_tag="ACHWN2_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR8932520.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8214..9227
                     /locus_tag="ACHWN2_003082"
     CDS             8214..9227
                     /locus_tag="ACHWN2_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR8932521.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9224..10054)
                     /gene="amiD"
                     /locus_tag="ACHWN2_003083"
     CDS             complement(9224..10054)
                     /gene="amiD"
                     /locus_tag="ACHWN2_003083"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR8932522.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10051..10374)
                     /locus_tag="ACHWN2_003084"
     CDS             complement(10051..10374)
                     /locus_tag="ACHWN2_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR8932523.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10585..>11016)
                     /locus_tag="ACHWN2_003085"
     CDS             complement(10585..>11016)
                     /locus_tag="ACHWN2_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR8932524.1"
                     /translation="CLFHECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARD
                     IYNMNAGNNVKVAADAFLEIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIE
                     ILLLSCFAAADQKAIQRYRPQSQDGEDFRSHLQYKLPLSAH"
BASE COUNT         2529 a         2915 c         2893 g         2679 t
ORIGIN      
        1 cccctctgat gcataacatt tccttttaat tgcaacttta ctcgcccttc cgctttcagt
       61 agtgtgtcat cgcgcatttc gacttcttct ttgcaaggac tggattttca tgttttctgg
      121 gctgttaatc attctggttc ccctgattgt gggttacctc attccgcttc gccaacaagc
      181 tgcgttaaaa gctattaatc agctattaag ctggatggtt taccttattc tcttttttat
      241 gggtatcagt ctggcgtttc tcgataacct cgccagtaac ctgttggcga ttctgcatta
      301 ttctgccgtc agtattaccg ttattttact gtgtaatatt gccgccctga tgtggctgga
      361 gcgaggcctg ccgtggcgca accaccatca gcaagaaaaa ctcccgtcgc gtattgcgat
      421 ggcgctggag tcgctaaaac tgtgcggcgt agtagtgatt ggttttgcca ttggtctgag
      481 tggactggct ttcttacaac acgcgaccga agccagtgaa tacacgttaa ttttgctact
      541 tttcctcgtt ggtattcagt tgcgcaataa tggcatgacc ttaaagcaga ttgtccttaa
      601 tcgccgggga atgattgtcg ccgtggtggt ggttgtcagt tcattaattg gtggtttaat
      661 taacgccttt attcttgatc tccccatcaa taccgcgctg gcaatggcct ccggtttcgg
      721 ctggtattct ctttccggta ttttattgac cgaatctttt ggtccggtaa tcgggagcgc
      781 ggcgtttttt aatgatctgg cccgtgaact gattgctatt atgttgatcc ctgggctgat
      841 tcgccgcagc cgctctactg cactgggctt atgcggtgcc acatcaatgg atttcaccct
      901 gcccgttctt caacgtactg gcgggctgga tatggtcccg gcggcaattg ttcacggttt
      961 tattcttagc ctgttagtgc cgatcctcat cgcctttttc tctgcgtaat acctctctgg
     1021 cggtagttcc ctgccgccaa aattgcgcta aatcaatctc ccttaaagtt gcatgaaaaa
     1081 tcccttttat ccccgcgtta agcgtcttac ccttaaacat gtatattaaa tataacttta
     1141 aaaggtgtga tcatgttttg tgtgcaatgt gaacaaacta tccgtactcc ggcagaaaac
     1201 ggctgctcat acgcgcaggg gatgtgtggt aaaacggcgg aaacttctga ccttcaggat
     1261 ttactcatcg cggcgctgca agggctttct gcctgggcgg taaaagcgcg tgaatacggc
     1321 atcatcaacc acgatgttga cagcttcgcg ccacgtgcct ttttctcaac cctgaccaac
     1381 gttaacttcg attctccgcg tattgtcggc tacgctcgtg aagcgattgc cttgcgcgag
     1441 gcgctgaaag cacaatgcct ggctgtagat gccaacgcgc gcgtcgataa cccgatggct
     1501 gacctgcaac tggtgagcga tgatctcggc gagctgcaac gtcaggcagc agaatttacc
     1561 cctaacaaag ataaagcggc gattggcgaa aacattctcg gcctgcgtct gctgtgcctg
     1621 tatggcctga aaggtgcggc ggcctatatg gaacacgcgc atgttctcgg tcaatacgac
     1681 aacgatattt atgcccagta ccataaaatc atggcgtggc tggggacctg gcctgccgat
     1741 atgaacgcgc ttcttgagtg ttcaatggaa atcggccaga tgaacttcaa agtgatgagc
     1801 attctggatg caggcgaaac cggtaaatac ggtcacccga cgccaaccca ggtcaacgtc
     1861 aaagcgacgg cgggtaaatg cattctgatt tccggtcacg atctcaaaga tctctacaac
     1921 ctgctggaac agaccgaagg cacgggcgtt aatgtctaca cccacggcga aatgctgcct
     1981 gcgcatggct acccggagct gcgtaaattc aagcatctgg tcggtaacta cggcagcggc
     2041 tggcagaatc agcaagtgga gttcgctcgt ttccctggcc ccatcgtgat gacctcgaac
     2101 tgcatcatcg acccaaccgt aggcgcttat gacgatcgta tctggacccg cagcatcgtt
     2161 ggctggcctg gcgtgcgtca tctggatggc gaagatttct ctgcggtcat cgcccaggcg
     2221 caacaaatgg cgggcttccc gtacagcgaa attcctcacc tgatcaccgt gggctttggt
     2281 cgccagacgc tgcttggtgc agccgatacg ctgattgatc tggttagccg tgaaaaactg
     2341 cgtcatatct tcctgcttgg tggctgtgac ggcgcacgcg gcgagcgcca ctacttcacc
     2401 gatttcgcca ccagcgtgcc ggatgactgc ctgatcctga ccctcgcctg tggtaaatat
     2461 cgctttaaca aacttgagtt tggcgatatc gaaggtctgc cgcgtctggt agatgcaggt
     2521 caatgtaacg atgcttactc agcaattatt ctggctgtca ctctggcaga gaaactgggc
     2581 tgtggtgtga acgatctgcc gctgtcgctg gtgctctcct ggtttgaaca gaaagcgatt
     2641 gtcattctgc tgacgctgct ttctctgggc gtgaaaaata tcgtcaccgg cccgactgca
     2701 cctggtttcc tgacaccaga cctgctggcg gtgctgaacg agaaattcgg cctgcgttct
     2761 atcaccactg ttgaagaaga catgaagcaa ctgttgaacg cgtaaggagt ttgtcgatga
     2821 cgatgccaac gaatcaatgc ccgtggcgga tacaggttca tcacattacg caagaaacgc
     2881 cggatgtgtg gacgatttcc ctgatttgcc acgatcacta cccatatcgc gccgggcaat
     2941 atgcactggt cagcgtgcga aactcagcgg aaacgctgcg tgcttacacc atttcctcca
     3001 cgccaggcgt gagtgaatat atcaccctga ccgtgcggcg gattgatgac ggtgtcggct
     3061 cccagtggct gacgcgcgat gtaaaacgcg gtgattatct ctggctttcg gacgcgatgg
     3121 gggaatttac ctgcgacgat aaagcagaag ataaattcct gttgctggcg gcaggctgcg
     3181 gcgtcacgcc gattatgtcg atgcgtcgct ggcttgcgaa gaaccgtcca caggccgatg
     3241 tgcgggtgat ctacaacgtg cgtacgccgc aggatgttat tttcgccgat gagtggcgta
     3301 actatccggt aacgctggtg gcagaaaata acgttaccga aggctttatc gctggtcgcc
     3361 tcactcgcga actgctggca gatgtacctg acttagcttc acgtaccgtg atgacctgcg
     3421 gcccggctcc gtatatggat tgggtagagc aggaagtgaa agcgctcggc gtgacgcgtt
     3481 tctttaaaga gaaattcttc accccagtag cagaagcggc gaccagcggt ctgaaattca
     3541 ccaaactgca accggcacga gaattttacg ccccggttgg caccacgcta ctggaggcgc
     3601 tggaaagcaa taacgttccg gttgtcgctg cctgccgtgc gggtgtttgc ggctgctgta
     3661 agacaaaagt ggtttccggt gaatatacgg tgagcagtac aatgacgctg accgacgccg
     3721 aaatcgctga aggttacgta ctggcctgct cctgccatcc gcagggggat ttggttctcg
     3781 cataatcgcc ttatgcccga tgatattcct ttcatcgggc tatttaaccg ttagtgcctc
     3841 ctttctctcc catcccttcc ccctccgtca gatgaactaa acttgttacc gttatcacat
     3901 tcaggagatg gagaaccatg aaacaaacgg ttgcagctta tatcgccaaa acactcgaat
     3961 cggcaggcgt gaaacgcatc tggggagtca cgggcgactc tctgaacggt cttagcgaca
     4021 gtcttaatcg catgggcacc atcgagtgga tgtccacccg ccatgaagaa gtggcggcgt
     4081 ttgccgctgg cgctgaagca caactttcag gagaactggc ggtctgtgcc ggatcgtgcg
     4141 gcccaggcaa cctgcacttg atcaacggcc tattcgattg ccaccgcaat aacgttccgg
     4201 tactggcgat tgccgctcat attccctcca gcgaaattgg cagcggctat ttccaggaaa
     4261 cccacccaca agagctattc cgcgaatgta gtcactattg cgagctggtt tccagcccgg
     4321 agcagatccc acaagtgctg gcaattgcta tgcgcaaagc ggtgcttaac cgtggcgttt
     4381 ccgttgttgt attaccgggc gacgtggcgt taaaacctgc gccagaaggg gcaaccacgc
     4441 actggtatca tgcgccacag ccagtcgtga tgccggaaga agaagagtta cgcaaactgg
     4501 cgcaactgct gcgttattcc agcaatatcg ccctgatgtg tggcagcggc tgcgcggggg
     4561 cgcataaaga gttagttgag tttgccggga aaattaaagc acctattgtt catgccctac
     4621 gcggtaaaga gcatgtcgaa tacgataatc cgtatgatgt tggaatgacc gggttaatcg
     4681 gattctcgtc aggtttccat accatgatga acgccgacac gttagtgcta ctcggtactc
     4741 agttccccta ccgtgcgttc tacccgaccg atgccaaaat tattcagatt gatattaatc
     4801 cagccagcat cggcgcgcat agcaaggtag atatggcgct ggtcggcgat atcaaatcaa
     4861 ccctgcgcgc attgctgcca ctggtggaag aaaaaaccga tcgcaagttt ctggataaag
     4921 cgctggaaga ttaccgcgat gcccgcaaag ggctggacga tttagctaaa ccgagcgaga
     4981 aagccattca cccgcaatat ctggcgcagc aaattagtca ttttgccgcc gatgacgcca
     5041 tctttacctg tgacgtcggc acgccaacgg tatgggcggc acgttatctg aaaatgaacg
     5101 gcaagcgtcg cctgttaggt tcgtttaacc acggttcgat ggctaacgcc atgccgcagg
     5161 cgttgggtgc gcaggcaacc gagccggaac gtcaggtggt cgccatgtgc ggcgatggcg
     5221 gttttagcat gttgatgggc gatttcctct cagtagtgca gatgaaactg ccggtgaaaa
     5281 ttatcgtctt taataacagc gtgctgggct ttgtggcgat ggagatgaag gccggaggtt
     5341 acctgacaga cggtactgag ctgcacgaca ccaactttgc ccgcattgcc gaagcgtgcg
     5401 gcattacggg tatccgtgta gaaaaagcct ctgaagttga tgaagccctg caacgcgcct
     5461 tctctatcga cggtccggtg ttggtggatg tggtggtcgc caaagaagaa ttagccattc
     5521 caccgcagat caaactcgaa caggccaaag gttttagcct gtatatgttg cgcgcaatta
     5581 tcagcggacg cggcgatgaa gtgatcgaac tggcgaaaac gaactggcta aggtaaaaag
     5641 ggtggcattt cccgtcataa taaggacatg ccatgattga tttacgcagt gataccgtta
     5701 cccgaccgag ccgcgccatg ctcgaagcga tgatggccgc cccggttggg gacgacgttt
     5761 acggagacga ccctaccgtt aatgctctgc aggactatgc agcagagctt tccggtaaag
     5821 aagccgccat ttttctgcct accggcactc aggccaacct ggtcgctctg ctcagtcact
     5881 gcgaacgtgg cgaagagtat attgtcggtc aggccgcgca taactatctg tttgaagccg
     5941 gtggcgcagc ggtgctgggc agtattcagc cgcaacccat cgacgcggct gccgacggca
     6001 cgctaccgct ggataaagtg gcgatgaaaa tcaaacccga cgatatccat ttcgcccgca
     6061 ccaaattact cagtctggaa aacacccaca acggcaaagt gctgccgcgt gaatacctga
     6121 aagaagcatg ggaatttacc cgcgagcgca atctggcgct gcatgttgac ggtgcgcgca
     6181 tctttaatgc cgtggtggct tacggctgcg aactgaaaga gatcacgcaa tattgtgatt
     6241 cgttcaccat ttgcctgtcg aaaggtcttg ggacgccagt cggttcatta ctcgtcggta
     6301 atcgtgatta cattaaacgt gccatccgct ggcggaaaat ggccggtggc gggatgcgtc
     6361 agtccgggat tctggctgcc gccgggatgt atgccctgga aaataacgtc gcacggttgc
     6421 aggaagatca cgacaacgcc gcctggatgg cggagcaact gcgtgaagca ggcgcggatg
     6481 tgatgcgtca ggacactaat atgctgtttg ttcgcgtcgg ggaagaaaat gctgccgcgt
     6541 taggcgaata catgaaagcg agaaacgtgc tgattaacgc ctcgccgatt gtccgcctgg
     6601 tgacgcatct tgacgtctcg cgcgaacaac tggcggaagt cgccgcccac tggcgcgcat
     6661 tcctggcgcg ttaaggagag agacgtgccg caacgcattt tagttctcgg tgccagtggc
     6721 tacattggtc agcatctggt gcgcacactc agccagcaag ggcatcagat cctagcggcg
     6781 gcacgtcatg tcgacaggct tgcaaagctg caactggcaa acgtcagttg ccataaagtc
     6841 gatctcagct ggccggataa ccttccggcc ctgttgcagg atatcgatac cgtctatttt
     6901 ctggtgcaca gcatgggcga aggcggcgat tttatcgctc aggagcgcca gctggctctc
     6961 aacgtccgcg atgcgctacg tgaagtacca gttaagcaat taatctttct cagttcgttg
     7021 caggccccgc cacatgagca gtcggatcat ctgcgagctc gtcaggctac ggcggacatt
     7081 cttcgtgaag cgaatgtacc agtgaccgaa cttcgggccg gaattatcgt tggcgcaggt
     7141 tcagcggcgt tcgaagtcat gcgcgatatg gtctacaacc tgccagtgtt aacgccgcca
     7201 cgctgggtac gttcacgcac cacgcccatc gcgctggaaa acttgctgca ctatctggtg
     7261 gcgctgttag accatccagc caacgaacac cgcatcttcg aagccgccgg accagaggtg
     7321 ctcagttatc agcaacagtt tgaacatttt atggcggtga gcggtaagcg ccgctggttg
     7381 atccccatcc ccctccccac ccgctggatt tcggtgtggt ttctcaatgt gattacttcc
     7441 gtaccgccca ccaccgccag ggcgttgatt caggggctga aacacgatct gctggcagat
     7501 gataccgcgc tacgtgcact catcccacaa cggctgatcg ctttcgatga cgcggtacgt
     7561 agcacgttga aagaggaaga aaaactggtc aactccagcg actggggcta cgacgctcag
     7621 gcctttgccc gctggcgacc ggagtacggt tatttcgcca aacaggcggg gtttaccgtt
     7681 aaaacgtccg ccagccttgc ggctttatgg ctggtggtga accaaatcgg cggtaaagag
     7741 cgttatttct ttggcaatat tttgtggcag acacgggcgt tgatggaccg cgcgatcggt
     7801 cataatctgg cgaaaggccg cccggagcgc gaatatttac agaccggcga tgcggtggat
     7861 agctggaaag tgattgtcgt tgaaccgaaa aagcaactta cgttgttatt tggcatgaaa
     7921 gcgccggggc tgggacgact gtgttttacc ctggaagata aaggtgacta tcgtactatc
     7981 gatgtccgcg ctttctggca tccgcacggt atgccggggc tgttttactg gttattgatg
     8041 atccccgcgc atctgtttat ttttcgcgga atggcaaaac aaatcgccag actggcagaa
     8101 caaagcacag attaatcgac aaaacctgcg ttattctttc atgtttccca ttgcatcatg
     8161 ggggaaatca cggaagaatg cgcacgtcat tttctcaaca acagtggatt gatatgaagg
     8221 tactggttac cggcgccacc agcggcttag gtcgaaacgc ggtagagttt ttatgccaga
     8281 aaggcatcag cgtgcgtgcg agcggtcgca acgaagcaat gggtaaattg ctggaaaaaa
     8341 tgggcgcaga gtttgttccg acggatctga ccgagctggt ttcatcacaa gctaaagtga
     8401 tgctcgcggg cattgatacg ctgtggcact gctccagctt tacctcaccc tgggggacac
     8461 aacaggcttt cgatctggct aacgttcgcg ccactcgccg cctgggtgaa tgggctgtcg
     8521 cctggggtgt gcgtaacttt attcatatct cttccccctc cctgtacttc gattatcacc
     8581 accatcgcaa tattaaagaa gattttcgcc ctcaccgctt cgccaacgag tttgcccgca
     8641 gcaaagcagc cagcgaagaa gtgatcaata tgctttcgca ggcgaatcca caaacgcgct
     8701 ttactattct gcgcccacaa agtctgttcg gaccgcacga taaagtcttt attccccgtc
     8761 tggcgcatat gatgcaccac tacggcagta ttctgttacc gcatggcggc agtgcgctgg
     8821 tcgatatgac ctactatgaa aatgccgtgc acgcaatgtg gctggcaagc caggaagcct
     8881 gcgataagct accttccggg cgtgtgtaca acatcaccaa cggcgagcat cgcacactgc
     8941 gcagcatcgt gcagaagctg atcgacgagt tgaatattga ctgtcgtatt cgttccgtcc
     9001 cttacccgat gctggatatg atcgcccgca gcatggagcg tttaggccgc aagtcagcaa
     9061 aagagccgcc gctgacccac tacggcgcct ccaagttgaa ttttgacttt acgctggata
     9121 ttacgcgtgc gcaggaagag ttaggttatc agccggtcat caccctggat gaaggcatcg
     9181 agaaaactgc cgcctggcta cgcgaccacg gaaaactgcc gcgctaatcc tgcccgtatt
     9241 tctccagcaa tgcttcggca atcgcctgag tttctgcatc cgcttcgccg ttatataacg
     9301 tcgggcggaa atgcatctgg aatgccataa tcacgcgccg ctgctcgcgc ggtgtcatat
     9361 caggttttac gtcataaccg tagcgcgcca aaagctccag caatgacgcg gtatctaccg
     9421 gtgtgtgcgg cgcgcgcccg gcaaggtaaa agttcacccg ctgcgcatcc ggccaggcac
     9481 caatcccctg ctgcgccagt tgctgccagg gaaataatgg ccccggatcg tctttgcgct
     9541 gcggtgcgat atccgcatgt gccactacgt tttccggctt gatgtgataa cgggcaataa
     9601 tatctttcgc cagcggaata agcgcctgaa tctgtgcctg ttcaaacggg gcaaaatatt
     9661 tcactccgga tgatttttgc cagccacggt tttccagctc aatgccaata gaggtgtcgt
     9721 taaggcgcgt tgccccgcgc caggcgctaa tccccgcatg ccaggccagt tcttgttccg
     9781 gcaccagttg ccagatgcgc ggtttaccgt tgtatcgcgg tggcaccgca gggatcagat
     9841 aatgcgagct gacctgctta tcagtcagtg tcgccagcga gctatcaaaa tcatctgcgg
     9901 tgtagtggat caccagcact ttaatgcgcg gatacgccgc ctgcgcctgg tgtcgggtat
     9961 caagctgata tccctctttc tcgacaatgc ctttttcgcc tgcacaccct gccagtaaca
    10021 gagcggcagc gaccagccag aagactcttc tcatcgacgc gttttcaccg ccgtaccgct
    10081 gacgctaacc atcagcatac tgccgttttg cccgaccgtt tcgtagtcga tatcaatacc
    10141 aactacggca tcggccccca gcgcccgcgc ctgggagcct aattcctcaa aggcgatctc
    10201 ccgtgcttta cgcagttctt tttcatacgc accggaacgt ccgccaacga tatcgcggat
    10261 accggcaaag aaatcacgga aaatattggc acctaaaatc gcttcgccgg tcaccacacc
    10321 gcaatattca acgatggtca ggccttccag agttggggtt gtagaaaatt gcatgatcga
    10381 tctccttgtt tgttaagcct tcgatctcta aagcattatc ggactgatat gccatgttgg
    10441 gaagaaggtt ttgatcagtt taggaaatca tctcattgtg tcaaaaacac ccacttaagt
    10501 ctttacgatt atccgttgca taacccgata ttggtttcat tatagggtac cgcttacctg
    10561 tcttattatg ttgcaccaac aacattaatg agcggaaaga ggcaacttat attgtaaatg
    10621 actcctaaag tcttccccat cttgtgactg aggtctatac cgctggattg ctttttggtc
    10681 agccgccgca aaacaagaaa gtaataagat ctcaatgctt ttgtcattaa tataagcgaa
    10741 gatcattctt gcaaactcgt atataaacgc ccgatcctga gcgcttaata gtttaataca
    10801 atatttattt ttaagagaga aaataatttc aaggaaagca tccgcagcaa ccttcacgtt
    10861 attcccggcg ttcatattat atatatccct tgcacagata gagtacagag atagtaaaca
    10921 gccatacaaa taatcactta ttaattcttt tgtccatttt acaggcaacc agggttttat
    10981 cttgacacca tgcatactac attcatgaaa aagaca
//
LOCUS       DAZSNO010000126        10894 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZSNO010000126 DAZSNO010000000
VERSION     DAZSNO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10894
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.126"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            71..832
                     /gene="adiY"
                     /locus_tag="ACHWN2_003086"
     CDS             71..832
                     /gene="adiY"
                     /locus_tag="ACHWN2_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR8932525.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            969..2306
                     /gene="adiC"
                     /locus_tag="ACHWN2_003087"
     CDS             969..2306
                     /gene="adiC"
                     /locus_tag="ACHWN2_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR8932526.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2410..4053
                     /gene="eptA"
                     /locus_tag="ACHWN2_003088"
     CDS             2410..4053
                     /gene="eptA"
                     /locus_tag="ACHWN2_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR8932527.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            4050..4718
                     /gene="basR"
                     /locus_tag="ACHWN2_003089"
     CDS             4050..4718
                     /gene="basR"
                     /locus_tag="ACHWN2_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR8932528.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4719..5819
                     /gene="pmrB"
                     /locus_tag="ACHWN2_003090"
     CDS             4719..5819
                     /gene="pmrB"
                     /locus_tag="ACHWN2_003090"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR8932529.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5795..5884)
                     /gene="pmrR"
                     /locus_tag="ACHWN2_003091"
     CDS             complement(5795..5884)
                     /gene="pmrR"
                     /locus_tag="ACHWN2_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR8932530.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5996..7498)
                     /gene="proP"
                     /locus_tag="ACHWN2_003092"
     CDS             complement(5996..7498)
                     /gene="proP"
                     /locus_tag="ACHWN2_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR8932531.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7762..8640)
                     /locus_tag="ACHWN2_003093"
     CDS             complement(7762..8640)
                     /locus_tag="ACHWN2_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR8932532.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEGLRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(8637..10865)
                     /gene="crfC"
                     /locus_tag="ACHWN2_003094"
     CDS             complement(8637..10865)
                     /gene="crfC"
                     /locus_tag="ACHWN2_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR8932533.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2724 a         2858 c         2680 g         2632 t
ORIGIN      
        1 tataaccatt tcccggtatc gctttgcttt agcgagaact ggtcttttat tcgcactcag
       61 gagtacatgt atgaggattt gcagcgacca accttgtatt gttttattga ctgaaaaaga
      121 tgtctggata agggtgaatg ggaaagaacc tattagcctt aaagctaacc atatggcgtt
      181 attaaattgt gaaaataata ttatcgacgt ctcctctctt aacaacactt tggttgctca
      241 tattagtcac gacatcatca aagattacct ccggtttctg aataaagatc tctcgcaaat
      301 accagtatgg caacgtagcg ctacgcccat actcaccctg ccatgcctga cgccagacgt
      361 ctttcgcgtt gccgcgcaac acagcatgat gcccgcagaa actgagtcag aaaaggaacg
      421 aacacgcgca ttattattca cggtgctatc ccgttttctc gacagtaaaa aattcctttc
      481 attaatgatg tatatgttac gtaattgtgt aagtgacagc gtttatcaaa ttattgaaag
      541 cgatattcac aaagactgga atcttagtat ggtagccagt tgtttatgtc ttagcccaag
      601 tctgttaaag aaaaagttga aaagcgaaaa caccagttat agccaaataa tcaccacctg
      661 ccgcatgcgt tatgccgtaa atgaattaat gatggacggt aaaaatatct ctcaggtatc
      721 acagtcctgc ggctacaaca gtacgtcgta ctttatttct gtctttaaag acttctacgg
      781 tatgacgccg ctgcattatg ttagtcagca cagagaacgc actgtcgcct gatttttaac
      841 cttaacgaag agctatatta ataacggcat cagcgataac ccggtcgata ataattcaac
      901 tatcgaatgc aggcgtatga tatgacgtaa tttattgtca cgaagctcgc cttcgcagga
      961 gtttaattat gtcttcggat gctgatgctc acaaagtggg cttaatcccc gtcaccctga
     1021 tggtgtcggg gaatattatg gggtcaggtg tttttctgtt acctgcaaac ctggcctcca
     1081 ctggcgggat tgccatttat ggatggttgg tgacgattat cggtgcgctg gggctctcga
     1141 tggtatacgc caaaatgtcg ttcctcgacc caagtcctgg tggttcttac gcttacgccc
     1201 gccgctgctt tggcccgttt ctcggttatc aaaccaacgt cctctactgg ctggcctgct
     1261 ggatcggcaa tatcgccatg gtggtcattg gcgtaggata tttaagttac ttcttcccga
     1321 ttctgaaaga tccattggta ttaaccatca cctgcgtcgt ggtgctgtgg atcttcgtcc
     1381 tgctgaacat tgtcggtccg aaaatgatca cccgcgtgca ggccgttgcc actgtgctgg
     1441 cgctgatccc catcgtcggg attgccgtat ttggctggtt ctggttccgt ggtgaaacct
     1501 atatggcggc atggaacgtc agcggcctgg gcaccttcgg tgcgattcaa agtaccctta
     1561 acgttacgct gtggtcgttc atcggtgtgg aaagtgcctc cgttgccgca ggtgtggtga
     1621 aaaacccgaa acgcaatgtc cctatcgcca ccattggtgg ggtattgatt gccgccgttt
     1681 gctatgtgct ctccaccacc gcgattatgg ggatgatccc taatgccgca ctgcgcgttt
     1741 ctgcctcgcc attcggtgat gccgcacgga tggcgttggg tgacaccgct ggggccattg
     1801 tttccttctg cgcagctgcg ggttgcttag gttcactggg cggctggacg ttgctggcgg
     1861 gtcaaacggc gaaagccgct gccgatgacg gactgttccc accgattttt gcccgtgtaa
     1921 ataaagcggg tacgccagtg gcggggctga ttatcgtcgg tattttgatg accatcttcc
     1981 agctcagcag catttcacca aacgcgacca aagagttcgg tctggtttct tccgtctcgg
     2041 tcatctttac actggtgcca tatctttaca cctgtgcggc gttactgctg ctcggccacg
     2101 gtcactttgg taaagcacgc ccggcatatc tggctgttac taccattgcc ttcctctact
     2161 gcatttgggc cgtggtgggg tccggggcga aagaggttat gtggtcattt gtcaccctga
     2221 tggtcatcac cgccatgtat gcgctgaatt acaaccggct acataaaaac ccgtatccct
     2281 tagatgcacc aataagcaaa gattaattcc ccttaatcca gcaaacataa aagccaacct
     2341 taagaactta aggttggctt aattttgctt tgcgagcata tgcgcacttt gttcgatgga
     2401 aacaccgtga tgttgaagcg cctactaaaa agaccctctt tgaatttact cgcctggcta
     2461 ttgttggccg ctttttatat ctctatctgc ctgaatattg ccttttttaa acaggtgttg
     2521 caggcgctgc cgctggattc gctgcataac gtactggttt tcttgtcgat gccggttgtc
     2581 gctttcagcg tgattaatat tgtcctgaca ctaagctctt tcttatggct taatcgacca
     2641 ctggcctgcc tgtttattct gattggcgcg gctgcacaat atttcataat gacttacggc
     2701 atcgtcatcg accgctcgat gattgccaat attattgata ccactccagc agaaagttat
     2761 gcgctgatga caccgcaaat gttattaacg ctgggattca gcggcgtgct tgctgagctg
     2821 attgcctgct ggctaaaaat caaacctgcc acctcgcgtc tgcgcagtgt tcttttccgt
     2881 ggagccaata ttctggtttc tgtactgctg attttgctgg tcgccgcact gttttataaa
     2941 gactacgcct cgttgttccg caataacaaa gagctggtga aatccttaag cccctctaac
     3001 agcattgttg ccagctggtc atggtactcc catcagcgac tggcaaatct gccgctggtg
     3061 cgaattggtg aagacgcgca ccgcaacccg ttaatgcaga acgaaaaacg taaaaatttg
     3121 accatcctga ttgtcggcga aacctcgcgg gcggagaact tctccctcaa cggctacccg
     3181 cgtgaaacta acccgcggct ggcgaaagat aacgtggtct atttccctaa taccgcatct
     3241 tgcggcacgg caacggcagt ttcagtaccg tgcatgttct cggatatgcc gcgtgagcac
     3301 tacaaagaag agctggcaca gcaccaggaa ggcgtgctgg atatcattca gcgagcgggc
     3361 atcaacgtgc tgtggaatga caacgatggc ggctgtaaag gtgcctgcga ccgcgtgcct
     3421 caccagaacg tcaccgcgct gaatctgcct gatcagtgca tcaacggcga atgctatgac
     3481 gaagtgctgt tccacgggct ggaagagtac atcaataacc tgcagggtga tggcgtgatt
     3541 gtcttacaca ccatcggcag ccacggtccg acctattaca accgctatcc gccgcagttc
     3601 aggaaattta ccccaacctg cgacaccaat gagatccaga cctgtaccaa agagcaactg
     3661 gtgaacactt acgacaacac gctggtttac gtcgactata ttgttgataa agcgattaat
     3721 ctgcttaaag aacatcagga taaatttacc accagcctgg tttatctttc tgaccacggt
     3781 gaatcgttag gtgaaaatgg catctatctg cacggtctgc cttatgccat cgccccggat
     3841 agccaaaaac aggtgccaat gctgctgtgg ctgtcggagg attatcaaaa gcggtatcag
     3901 gttgaccaga actgcctgca aaaacaggcg caaacgcaac actattcaca agacaattta
     3961 ttctccacgc tattgggatt aactggcgtt gagacgaagt attaccaggc cgcggatgat
     4021 attctgcaaa cttgcaggag agtgagtaaa tgaaaattct gattgttgaa gacgatacgc
     4081 tgttattgca gggactgatt ctggcggcgc aaaccgaagg ctacgcgtgc gatggcgtga
     4141 caaccgcgcg gatggcggaa caaagccttg aggacggtca ttacagcctg gtggtactgg
     4201 atttagggtt acccgacgaa gatggactgc attttctcgc ccgtatccgg cagaaaaaat
     4261 acaccctgcc ggtactgatc ctcaccgctc gcgatacgct gaccgacaaa atcgccgggc
     4321 tggatgtcgg tgccgatgac tatctggtga agccttttgc gctggaagag ttacacgccc
     4381 gtatccgcgc cctgctacga cgccataata atcagggcga aagtgagctg attgttggca
     4441 atctgacgct gaatatgggt cgccgtcagg tatggatggg cggtgaagag ttgattctga
     4501 cgcccaaaga atatgctctg ctgtcacggt taatgctcaa agcaggcagt ccggtacatc
     4561 gggaaattct ctacaacgac atctataact gggacaatga accctcgacc aacaccctgg
     4621 aagtgcatat ccacaatctg cgcgacaaag tgggcaaagc ccgtatccgc accgtgcgcg
     4681 gctttggcta tatgctggtc gcgaatgagg aaaactaatt gaatctgatg cgttttctgc
     4741 gccgacctat atcgctgcgc caacggctga tattgaccat cggggccatt ttgttggtgt
     4801 ttgagctgat cagcgtcttc tggctatggc atgaaagtac cgagcagatt cagctgtttg
     4861 agcaggcgct acgcgacaat cgcaacaacg atcgccacat catgcgtgag atccgcgagg
     4921 cggtcgccag cctgattgtc cccggcgtct ttatggtcag cctgacgcta tttatctgct
     4981 atcaggcggt acgccgcatc acccgcccgc tggcggagct gcaaaaagag ctggaagcgc
     5041 gcaccgccga caacttaacg cccattgcca ttcacagcgc caccctcgaa atcgaagcgg
     5101 tggtttcggc gttgaacgat ctggtcagtc gcctgaccag cacgctggat aacgaaaggt
     5161 tgtttaccgc tgacgtcgcg cacgaactgc gaacgccact ggcgggggtg cgtttgcatc
     5221 tggaactgct ggcgaaaacg catcacattg atgtagcacc gttagtggca cggcttgatc
     5281 agatgatgga gagcgtctcc cagctgctgc aactggcgcg tgccggacag tcattttctt
     5341 ccggtaatta tcaacatgta aaactgctgg aagatgtcat tctcccctcg tatgacgaac
     5401 tcagtaccat gctcgaccag cgacagcaaa ccctgctatt gccagagagc gccgccgaca
     5461 tcaccgttca gggtgatgca accctgctgc ggatgttatt gcgaaacctg gtagaaaacg
     5521 cccatcgtta cagcccgcaa ggcagcaaca ttatgattaa gctgcaagaa gatggcggag
     5581 cggtcatggc agttgaagat gaaggaccag gtattgatga gagtaaatgc ggggagttga
     5641 gtaaagcgtt tgtacgtatg gacagccgtt atggcgggat tggtctgggg ttaagtattg
     5701 tcagccgcat tacacagttg catcacgggc agtttttcct gcaaaaccgg caagagactt
     5761 ccggcacgcg ggcctgggta cggctgaaga aagatcagaa cgtggcaaac cagatataaa
     5821 gaaagctgct gaccaccagc acgctgaaca cggtagttaa actttcataa acacggtttt
     5881 tcattgcact ctcctccgtt aacctggagg agagtatgcg cgtcagagat taagtgaacc
     5941 ttaagagttc aattggcctg ataagacagc gtcacatcag gccatccgtt tcagcttatt
     6001 catcaattcg cggatgttgc tgcaccaggc gggtacgttt cgcctgcaaa tcggcaatct
     6061 cgtggtcaat attatcgatt ttctgctcga tattatcgta atgctcgacg agaatttcct
     6121 tcgcttcctg tatatctgac gccgccggtg tcgcaccttt caacggacga tttgccgtct
     6181 ctttcatggt tacgccggtg attaaaccaa tcaccgccac taccatcagg taataggcag
     6241 gcatcatcag attctgcgag ctttcgacca gccaggccgc cagtgttggc gtcagaccgg
     6301 caaccagcac cgaaatatta aatgccgccg ccagcgcgct gtaacggata tgcgtcggga
     6361 acatcgctgg caaggtagaa gccataacgc ccataaagca gttaaggatc accgccagca
     6421 tcagtaaccc ggcaaaaatc aggccgatga cgttactgtt aatcagaata aacgccggga
     6481 tcgccaacac aaacagtgca acactaccaa gtagcacaaa cggacgacgg ccaaaacggt
     6541 cactcagcaa gcccatcacc ggctggacaa acagcatacc gatcataatg gcgataataa
     6601 tcagcacccc gtggtcttcg gagtaatgca ggttatgcga caaataactc ggcatatagg
     6661 tcagcaacat gtagtaagtc acgttggtgg caattaccag accaatacat gtcaacaggc
     6721 tgcgccagta tttagtggca atctctttaa acgagacttt cgggccatcc tgcaaacctt
     6781 cgcggtcgcc ctgttccagt ttatcaacat gctgctggaa cgccggagtt tcttccagcg
     6841 catggcgcag gtaaagcccg ataatcccta acggcagagc aataaagaac ggaatacgcc
     6901 agccccagtc gaggaagttc gcttcgccga caatggtcga aattaacacc accacgcccg
     6961 cacccagcac aaacccggca atagaaccga agtccagcca gctgcccata aagccacgtt
     7021 tacggtcagg ggagtattcc gcaacaaata tcgacgcccc ggtatattca ccaccgaccg
     7081 agaaaccttg tgccatctta cagatcaaca gcagaatcgg tgcccaaata ccaatcgtgt
     7141 cgtaggacgg tattaagcca atacagaacg tactgatcga cataatcaca atagtgatag
     7201 cgaggatctt ctggcgacca tatttatcgc ccaacatacc aaagaagagt ccgccaagcg
     7261 gtcgaatcag aaagggaacg gagaaagtgg caagtgcagc aaccatctgc acgctggggt
     7321 cagcccccgg gaaaaaaact ttacctaatg cgtaagcaac aaaaccataa acaccaaaat
     7381 caaaccattc cattgcatta cccagtgatg ctgcggtaat ggctttacgc agtttaccgt
     7441 catcaataat ggtgacatca cgaagggtaa tcggttttac ttttttcctt ttcagcatag
     7501 ctttcctcgc agagttggcc catttccgca aacgccagaa aaatcagcgc gacggccgca
     7561 aaaggggagc gggaattgca ggatgcaatc tctgtaacaa gcgtagcggg tttgcttaca
     7621 ccctccggtt aaggaatgta caatcaaacc agttgacgcc tgaattgggc agttaatgac
     7681 ctttttaccc taccagtgtt taaatttgtg atcaacttca cacatggatt tcgtttagga
     7741 ctcattgatg taactgatta tttacgccaa atttacagag aagatttcct cgaccattgc
     7801 ccccaccaaa cgctgcgctg aacagagtcg tggcatcccc agcgcgacag tttgccagcg
     7861 ttgggtcact gaccagcaaa cgggttgttt atctgccgtg gtccactttc ccagccaact
     7921 taaaaaatca gcatgatccc cgcccgtcgg aacggtcgaa gaggcaaacc actgatggta
     7981 atagtgacgc gtagtcggtg ccgcattgat actctctgcc agatgttgtg tcgctttctc
     8041 gcgcaaatca cccagcaaca acaccaccgc ttgcgggctt cccagacggc aggcatcgcc
     8101 aaatgcaccc cagcgcaacc cctccagaac gataaaacag cgcccaggca acgaccaggc
     8161 gttatacgcc ccagcacgcc agcgggtaaa aatttgctcg ctctgatcac ctgcctgcac
     8221 cagtaggcct cgttgcgtta acgccttttc cttccagcga caacgggctg aaaaccactc
     8281 atacaaggcg gcaatttgct gggggagctc cgggaaagca gagtgctggc ggtgatccat
     8341 tttttgcagc agcgccagat gctgcgcgat ctgcgtcagc gccagatgac cgggatccag
     8401 catcgacgcc agtttttcca ccattgtcag tcgcaggcta agcggcgcat ttaacatgcc
     8461 gccgttcgtc catgtctgaa gctgcacacg cgaaagaagt tcctgcatca gtcgttcacg
     8521 aaactgctgc tgatgggctt gaggatggcg ggcatcatcg ccctgcgcca gatccactaa
     8581 aaagcgcgga tgaaccgact ccagcacgcg accggggccg tccagtaacg tcttggtcaa
     8641 tatcgttctg ccgtgaaaag tgtttgaata tcatcgcgta acagctgagc gtcggtgtaa
     8701 atccagctgt gagtcatcac agtctgctgc aattgctgct gcatcagcct gaccaccgat
     8761 tcattttgtt gacgcagagc cagactttcg cgtaaacgcg tctgtaattc tgtcaaacat
     8821 gaagcgaact cagcgaaaaa agtattcatg cctgccgtaa cagattcatc gacctgctct
     8881 gccagaactt tagccatttg ttggcaatag agatcgactt ctgcgcttaa tgctcgttgc
     8941 aacacactgt aatcaaccgt ttctgtcggg gatttctcat ttccccgtcc ccagtcgggc
     9001 tgattcaacc agcgcgaaaa agtctcacgc acaacgccta aacgcgtgct ctgctcgtcc
     9061 gttgcttcct ggcgcgaaat gacttcactg aacagctggc gagtgttgaa gtggggaact
     9121 acgccgtgaa aaacaggaaa atgaaaccca ggacgaaacc ctgactcgct caattccatt
     9181 ttgacttgtt gctcaatggg gcgaataaca tcggttaaca cccggcaaag ggtggattcc
     9241 atctcggcaa aacgcagcgt aaagtcgcga ctgatggtgt tctgcgccgt ctgtaacagt
     9301 gtctcacagc gggtacgaat ctcgcttaac ggctccgaat catcctgaaa caaggcggct
     9361 aactgcgcat tcaccgcatc ttgctgttga cgcagaaagt ggttggcgga ggtcagggcc
     9421 agctcgattt catgtttaat ctcgccgctc acctgcgcct gattgagttg caatagctgc
     9481 aaactttctt cgacctgatg gatattttgc cgcaattgtt cacaagcgac gtttaacccg
     9541 tgcgcacgaa aatccaggta ttcccgcgcc tgctgcgcgt aattcaacag tttatgcgca
     9601 gcagatcgca gagcatacaa tgaggcgtta gcgtaagcgg catgaagcaa cgcctgaatt
     9661 ggctgggcga acagcgaatc ttcccacaac tgatcggcag catgacgaat atgttcgagg
     9721 tccgccagat cggcatgacg ccagcgcctg ccgagcgcgg catgggcaaa atcttccacc
     9781 cagcgttgtt gctctggcgc tggtaactta ccgttgttgg ctaactcata gcgcgcccga
     9841 ttcgccaggt agccccacat cgacgacacc gggaatatct gctgtggcgt aatacagcct
     9901 ttcatcagcg tcccggaaat cagtgcccgc acctggtcgg cgtcgtcact gttacgatcc
     9961 tgttgatcga acttattgac cagcacatac agcggcaccg attgccccac cgccaaaatc
    10021 gcctcacgga cctcttcatc ggagatcgat ttcagttgcg tataatccag caccgccagt
    10081 accgccgagg cgcgtgccag ctgctggtta agcatttttt gcagatgcgg ttgcccggct
    10141 tcatttggcc cgggggtatc cagtaacgtc aactgaccgg gataactttc cagccccgcc
    10201 agatggacaa actccacttc aatcacggga atatgctcaa tggcggcgta agcagaaaaa
    10261 ggaaaatcga cgtccagcgc cttcgccagt cgcactaaat cattcaaact tttcagacaa
    10321 tgaaaaatag gctgggcacc cagataatat ttttcgaaag cgacgccatt ttcgatccgc
    10381 tgcataagcg cacgcatatc tttatctatt tccagcacat cggtcagatg cttaatatcg
    10441 caatcacgca ggcgctgttg taattgttga attaaacaat cgattggcgc gacatgtgaa
    10501 aaatgcagta ccggttcctt ttgcccgggc gtatggcgaa taagcgtcgg cagcgcagtc
    10561 attgggcgat tacgattagg cagaacctcc gtaccaacaa tggcattaat ggtggttgat
    10621 ttccctgctt tcatggtacc gacaattgcc agcaccattt ccagtcggga aattttacac
    10681 aactcattat tcagcatcgc gtgacgttcg gcgatattag gctgactcca gggtaaagcc
    10741 agttgtggcg cgtcgtctcc gggtacagag agaggcattt tttccagtaa ctgcaactgt
    10801 tggcgagaaa gctgtaacag gcgttcagcc tcctgactta actcatacag ggtctgtgtg
    10861 tacatagaaa attcttcctt aaagcaaatt ttgt
//
LOCUS       DAZSNO010000127        10848 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZSNO010000127 DAZSNO010000000
VERSION     DAZSNO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10848
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.127"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            210..1040
                     /locus_tag="ACHWN2_003095"
     CDS             210..1040
                     /locus_tag="ACHWN2_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR8932534.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMPYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1181..1954)
                     /gene="uxuR"
                     /locus_tag="ACHWN2_003096"
     CDS             complement(1181..1954)
                     /gene="uxuR"
                     /locus_tag="ACHWN2_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR8932535.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2169..3629)
                     /locus_tag="ACHWN2_003097"
     CDS             complement(2169..3629)
                     /locus_tag="ACHWN2_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR8932536.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3710..4894)
                     /gene="uxuA"
                     /locus_tag="ACHWN2_003098"
     CDS             complement(3710..4894)
                     /gene="uxuA"
                     /locus_tag="ACHWN2_003098"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR8932537.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5234..6577
                     /gene="gntP"
                     /locus_tag="ACHWN2_003099"
     CDS             5234..6577
                     /gene="gntP"
                     /locus_tag="ACHWN2_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR8932538.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6828..7730)
                     /gene="fimH"
                     /locus_tag="ACHWN2_003100"
     CDS             complement(6828..7730)
                     /gene="fimH"
                     /locus_tag="ACHWN2_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR8932539.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7750..8193)
                     /locus_tag="ACHWN2_003101"
     CDS             complement(7750..8193)
                     /locus_tag="ACHWN2_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8932540.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8266..8796)
                     /gene="fimF"
                     /locus_tag="ACHWN2_003102"
     CDS             complement(8266..8796)
                     /gene="fimF"
                     /locus_tag="ACHWN2_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR8932541.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8806..>10848)
                     /gene="fimD"
                     /locus_tag="ACHWN2_003103"
     CDS             complement(8806..>10848)
                     /gene="fimD"
                     /locus_tag="ACHWN2_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR8932542.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2606 a         2797 c         2660 g         2785 t
ORIGIN      
        1 aataagatat gcttttaaat tgcatgcaaa taattttaaa tgagcgtttt ttgatacttt
       61 agagtgtttc ctacccttta atatcactgt gtagagttga tatcactaca acagaaacat
      121 gcatgtcatt tgtttacttc tctttttact acagaaaaat ttatctattt ttctaaacat
      181 cagttaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat gccgctaata
      241 accaattcaa tacaaataat cacttccttc atcatccaaa agtcgacagt gagttaacac
      301 gtcgatatga atatgcacgc ctggatacgg aaaatatcta tcttctgcct cttgctcgtg
      361 gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt aatattggca
      421 aacagtcctg gccatttaat tatgtgacag atacatgcag agaattcaat ggtattacaa
      481 caacaggacg tatgccctat cgaaacttga aaatcacctc agctttagac gaaatatatg
      541 gtggtatatg taaaaaggcg catgctgcta cagagttagc agaaggttta cggctaaatt
      601 tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa ataacattag
      661 gccctggttg caatgttcct ggttatgccg gaaccacgat aggatatatt tcaacccttc
      721 cagcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc tatattgatc
      781 agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc gcattactta
      841 atgctaatat tgagatggga aatgacccaa tcattggtat agaagcctat cctggtaccg
      901 cggagatcca ttcaaaaatg ggatataacg ttatccccgg tgatgaagat gcgccactca
      961 aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa aatggtgaat
     1021 ggaattatat tggtaaatag ccactgagaa gaatgagtac taatattcag gttatacaga
     1081 tatttaaaca aaaccctgca atttacaaag taatactcat tgcgacttct taatttaaga
     1141 aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg actttatcca
     1201 gcggccatga atcaaagaga tagccgtcaa aataaatatc gtcaacgttc gagaattcca
     1261 gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc gctcgggcat
     1321 cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct ttgcgataca
     1381 ggctgtcatc cagatggctg tgcaactgga tccacattgg attgttttcc cgccactgcc
     1441 aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcagca atggcgagat
     1501 ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt tcacgctctt
     1561 ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc agtgcggcaa
     1621 actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa ggacccgcat
     1681 cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta tcaagcacat
     1741 agataccggc accccggcgc acttccacca gccctttgat ctccagcatg atcagcgctt
     1801 cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc ggcggcagcc
     1861 gttcgccagg attgtacggc gtctttatga tcagatcgtg gatcatcgcc ccgacttcct
     1921 ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt gtgtccgtta ggtgcggtta
     1981 atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg gcaattttac
     2041 ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta caggtgggca
     2101 aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc tgtagggtaa
     2161 tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag ctgctgatat
     2221 gccgacgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc aaaaataccg
     2281 ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt aatcttctgg
     2341 aactcggcca gcatcgggtc gaccacgtca atggcattac cctgctcatc cacgccctgg
     2401 gtgtaaccca tccagccagc cacgcccagc gccaggtgac gccagctgcc gccgttttgc
     2461 aggtgcagac gcaccgggtc cagtagacgc tgcggtaact tctggctgcc gtccatcgca
     2521 atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag cagcgtcgca
     2581 taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc ctgcatcatc
     2641 agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat ggtttcatag
     2701 ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat acgcagcttc
     2761 atcatttcga acggcacaac gtctgcaacg aactgtgtgc ccactttatc ccagtccggg
     2821 cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca ggcaatggcg
     2881 cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg cgtcgccgcc
     2941 ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat ccatgccgct
     3001 agctgcgggt cacgcgcctg agccagcccc agtaccgcga ccttcgccac atggccgttt
     3061 tcacgcacgt tatcgcagga catcaccgta aatgctttca gccctttttc acgacgcaga
     3121 cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt ttccagatcg
     3181 tgcttgatca gcgggttatt gagatccagt tgaccgcttg ccgcatcagc gcagtagcct
     3241 ttttccgtga ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc gttgagaata
     3301 ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat aattttcagc
     3361 tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt tttcaggttt
     3421 tcgatcagca tgcggtcgtt gcctggcatc aggttaactt cgcagatgcc ccagtcgctg
     3481 tcggtgcttt ccagcagatg atgggtatac agcgcctggt gcgcgcggtg aaacgccccg
     3541 caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg gcgggcaacc
     3601 ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct tcagagagtg
     3661 acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtgggtat taacggctaa
     3721 agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct ttcagacgac
     3781 caattgcgga gtaaccaggg ttggttttct tcttcaggtc gtccagcatc tgatgaccgt
     3841 ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg tgttcttctt
     3901 caacaatcgc tttcaccact ttgtacatat caacgtcacc gttcaggtgc gccgcttcgt
     3961 ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag taaatacgcg
     4021 gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg taggaaccgg
     4081 tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac tgcatatctt
     4141 caatggtgga aacaatgcgc ggcaagccga ggatcgggcg cggcggatcg tccgggtgaa
     4201 ccgccatacg cacgccaact tcttcagcaa ctggaataat cgctttcagg aagacggcaa
     4261 agttttcgcg cagtttggct ttgtcgatat ctttgtacag ctccaggtgc ttacggaact
     4321 ggtcgagggt atacccttct tccgcgcccg gcagaccggc aatgatgtta cgcgtcagac
     4381 gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc tgcctgagca atttcttctt
     4441 cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat gcagcgaatt
     4501 cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg aggtcagtac
     4561 gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgcca cactgcgcca
     4621 ggttgcgcag agtctgctga tagttagcga tccactgctc atagttgcca gtgtgggttt
     4681 tgatatcttc gtgaattggt acgctttcta cgacagacca caccaggcct gcgtcttcaa
     4741 cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc gggatatggt
     4801 gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa gaaaccggat
     4861 cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca catagtgggt
     4921 tccacagcaa agagacgcgg aaccttggag ttgtttaggt atccggatga acaaccagac
     4981 cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt ggtaagacat
     5041 gtcaaccaga attctgaaat tggttaaccg cgtcgcaaga atttcactct catggttgtt
     5101 gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg acattcatcg
     5161 caacaatggt tgaccaattt acataacatg tcgcccaaaa taacaacggt tcaaccaagt
     5221 aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca ttggcctgat
     5281 gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg tggcggcgct
     5341 gtccgtcggg atgctggcag gcatggattt gatgtcgctg ctgcacacca tgaaagcggg
     5401 cttcggcaac acgctgggag aactggctat catcgtggtg ttcggtgcgg tcatcggtaa
     5461 attgatggtc gactccggcg cggctcacca gatagcacat acgctgctgg cgcgtctcgg
     5521 tctgcgctat gtacagctgt cggtgattat catcggcctt attttcggtc tggcgatgtt
     5581 ctatgaagtg gcctttatca tgttagcgcc gctggttatt gttattgccg ccgaagctaa
     5641 aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg cacattcact
     5701 gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg atatggggat
     5761 ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag gtctgatcct
     5821 gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag cagatcaacc
     5881 ggtagatatg aataatctgc cctctttcgg cgtttcgatt ctggtgccgc tgatcccggc
     5941 gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata cccctgcctg
     6001 ggaagtggtt aactttattg gttcctcgcc gattgcgatg tttattgcga tggtggttgc
     6061 attcgtactc tttggcaccg cgcgtggtca tgacatgcag tgggtgatga acgcttttga
     6121 aagcgcggtg aagagtattg caatggtgat tctgatcatc ggtgcgggtg gcgtgctgaa
     6181 gcagaccatc atcgacaccg gcattggcga caccatcggc atgttgatgt cccacggcaa
     6241 tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg cgacgggtca
     6301 gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc ttgatccagc
     6361 aaccggtcag ctggttggcg tgaatccggc gctgctggta ctggcgacgg ctgcgggttc
     6421 caacaccctc acccacatta atgatgcatc tttctggttg ttcaaaggtt actttgacct
     6481 gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact ccgtggttgg
     6541 gctgattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag cctgccaatg
     6601 gcaggctcag actgatgact aaagtctaaa aatgtgcccg gacagtcccc tcgcccctcc
     6661 ggggagaggg ttagggtgag gggaacagac cagtaaagtg cgctttgttt atgccagatg
     6721 cgacgctgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt gtaggtctga
     6781 tagcgtagcg cctcaggtac cagcattagc aatgcgctgt aattttttta ttgataaaca
     6841 aaagtcacgc caataatcga ttgcacattc cctgcagtca cctgccctcc ggtacgtgcg
     6901 taatttgccg ttaatcccag acttaccgcc gaagttccta ctgctcctaa cgataccgtg
     6961 ttattcgctg gaataatcgt accgttgcgc gtcaactgta cgccgacgcc ctgcgctggt
     7021 gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt gccggagagg
     7081 taatacccca ggttttggct tttcgcacaa taaacggtaa gaggaattgg cactgaacca
     7141 gggtagtccg gcagagtaac ggtgacatca cgagcagaaa catcacaacc gccagtgggc
     7201 accaccacat cattattggc gtaaatattc cacacaaact gaaaatcatc gctgttatag
     7261 ttgttggtct gtcgcaaaat aagcacggca attaatgaac cagctttaat agccaccccg
     7321 cccgcactgc tcacaggcgt caaataaagc gccaccggcc acggcttatc cgttctcgaa
     7381 ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg gatagctact gccactatat
     7441 tttacggtcc cggaaaaatt agataacacg ccgccgtaag ccgagcctcg ttgcagtgtg
     7501 acatagtctg taatggtttc cggataatcg ttatggcaaa agatttgcgt cgaaagatct
     7561 acgaccaggt tttgccccac attcacgaca ggcgcaaggt ttacataaac attagcgctg
     7621 ccaccgccaa tagggatagc ggtaccattg gcggttttac aggcgaatga ccaggcattt
     7681 accgaccagc ccatcagcag tacagcaaac agggtaatag ctcgtttcat tacaattatc
     7741 tcttcgggtt cagctgtagg tataggtgat gctaatcact gcctgaatgg ttccctgagt
     7801 ggctccgcca tttaccgtca atgctctgac ctgtaacggg aagtgcgctg attgtgagga
     7861 atcatccacc tgaactgttt tggttgcgcc agtattcaat gtgttgccac tgtcatcctg
     7921 tagctctaac tggatgtttt gcgcggtccc ctggttttta taatatccgg tgctgtcggc
     7981 tgccccgctg aagctggcag tgaccctcga cgttcccacc ggacaattag tcaactcaag
     8041 cgcaacatca tgccaggccg atgccgcccc ggcagacata agactgaaag aataaagatc
     8101 gccgagatct accgtggcat tggtggtgga aaccgtacac ggtttggcga cgaccttacc
     8161 gttcaccgtg atggtgacat cggctgcctg tatcgtcgca cttgcgagcg ccaacattgc
     8221 cgccaataca tacccacgtt tgcaccattt catgagcatc tccagttact gatattcaag
     8281 agtgaaggta gccgtagcat tgatatgccc cgcagtgaca ggcacctgtg tcgccattag
     8341 ccgggcgtaa aaattcagcg tatttggttt acccggcgtc agggtcgtcc acgaaatcgc
     8401 agacgatgga gcattaaggg gtatttgatt ttgctgctca ttcagaagct gtattcccag
     8461 tcccgcagcc gctgacaccg tattttcaag tgcaagcagg ttggcattgt ggctatctgc
     8521 aacgccggta aacccaactt ttacggcaga aacggcatta ccacagggtg acagcaaaat
     8581 acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta aattgcttcg ccgcgttttc
     8641 catcagatca acagtaaaat tggttgattc agcggccaca ctacagccgt tatctctgac
     8701 atagccgcgg atagtaatcg tgctatccgc agccaaaacg cgacttaccg ccagccacaa
     8761 aaaagcgcac agaagataaa aaggtttgtt tctcatcacg cccccttaac gacattcagc
     8821 tgatagctgg gttaataact gctgctgact ctctggtggc agttgataat tggcgacaca
     8881 gtgagcattt tcctcttctc cccatttcac ctgaactttt cccgctaaag gcattccgct
     8941 gaggtaaacc tgaccattat ccgcaacaat gccgctactc tggctactct ctgatgtcac
     9001 catcgcccca aacggcagcg gcttattatt gtgagttagc gtcatgagca gttttatccc
     9061 aacgcgcgct ttaaactctg ctcgcacgat cgccccacga gtgggaacaa cgttagcgac
     9121 cgcgttatct aaatcgacgt tatcagccaa ggtattggta tccagcgcca ctctattttc
     9181 ccgatattca gtggcataag gcagcacggc ataaccacgc cagtcggtac gcacccccgt
     9241 ctggttttcg acttttgcat cttttgcgcc aggcgcttta acaagcacca ccgtatcgtt
     9301 taacggctgc cccagcgtta cgccattggc atgagccaat accccaccgc tgactccgta
     9361 atagagctgc ttaatatcat cgctatggct gtaaccgata ttggcattgc cgtaaccacc
     9421 gcgataattc agcgtggcgt agcctgtact tccgctatta ccatcgcctc ccccggcata
     9481 gccggtttgc acgctataac tgaggttgtt gtcttccagc aacgtaccgt atacaccagc
     9541 cagattggtc atccgaccgt tgagatcgtg tgacatgctg tagctggcac tggcatgtcg
     9601 ccactgagat ttactgtcag aacgcagcca gtggctgaaa ggaatattga cgttaagcgc
     9661 taacatctga tcccgtcctt tttgccaggc gtttttcgtc aggctatagc tgagcgtcca
     9721 gttgatatct tcgaacgcag tatttaatcc agcctggaat tgctcatcga cattactcgt
     9781 tccccaataa gtttgatggc taccactcaa atacagtgtt gatgtgcgcc cgagttgctg
     9841 agtaacggtg agctgtaatt tcccgcgttt gttataagcg aggttgtaat agtcggtgaa
     9901 tttcggctta acctgaataa ctccatcctg tgtttcgatg ttgtagccat tcattcgact
     9961 gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa taacggtaac ccactaactg
    10021 aatattcgtg cctgattcat tgagcgattt gttatagaga aaacgcaccg attgtccgtc
    10081 atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc atatccacag acagagcgcc
    10141 cagtgcctcc atgtttttcc cgataccgaa attaaaagca cgataacgat ccgccagttg
    10201 cgttccacca tatattgtcc agccagccgg aaggccgtgg agtaatgtac tctggaaaaa
    10261 gcggggtttt tcctgctgcg catttccact acggtattct cctgccgtaa tggaataacg
    10321 agtatgccct tcacgttgca aaagcgggac tgacgaatag ggtacggtaa aaatctgcgt
    10381 gctgccgtca gcctctttga tcgttacctg caagtcacca ctattacctg cggcatagat
    10441 atcgttgatg gtaaaaggcc ccggtggcac cgtactatta taaatgccat atccattttg
    10501 tttaatagtg acctgtgcag taccacgagc aataccgtgg atcaccgggg caaatcctct
    10561 ttggctatcg ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa agttaatacc
    10621 atcgaaaata tcgccctggg tataaccatc acccaacgtc agccgggaac gtaaaggaat
    10681 tatgtctcgc tcaatccagg taatgatatg ctgccattta tttttgctac ctgatgaact
    10741 gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa cgccacgcac caatatttaa
    10801 cccactctgt aggtttaaat atgcataatg gctgttaccc ccaatccg
//
LOCUS       DAZSNO010000128        10745 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZSNO010000128 DAZSNO010000000
VERSION     DAZSNO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10745
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.128"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            109..1509
                     /locus_tag="ACHWN2_003104"
     CDS             109..1509
                     /locus_tag="ACHWN2_003104"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR8932543.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            1665..3200
                     /gene="gadC"
                     /locus_tag="ACHWN2_003105"
     CDS             1665..3200
                     /gene="gadC"
                     /locus_tag="ACHWN2_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR8932544.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            3331..4650
                     /locus_tag="ACHWN2_003106"
     CDS             3331..4650
                     /locus_tag="ACHWN2_003106"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR8932545.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            5027..6097
                     /gene="dosC"
                     /locus_tag="ACHWN2_003107"
                     /pseudo
     CDS             5027..6097
                     /gene="dosC"
                     /locus_tag="ACHWN2_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            6097..6714
                     /locus_tag="ACHWN2_003108"
                     /pseudo
     CDS             6097..6714
                     /locus_tag="ACHWN2_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            6972..7304
                     /gene="ddpX"
                     /locus_tag="ACHWN2_003109"
                     /pseudo
     CDS             6972..7304
                     /gene="ddpX"
                     /locus_tag="ACHWN2_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            7318..8214
                     /gene="ddpC"
                     /locus_tag="ACHWN2_003110"
     CDS             7318..8214
                     /gene="ddpC"
                     /locus_tag="ACHWN2_003110"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR8932546.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8211..9185
                     /locus_tag="ACHWN2_003111"
     CDS             8211..9185
                     /locus_tag="ACHWN2_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR8932547.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            9178..10104
                     /locus_tag="ACHWN2_003112"
                     /pseudo
     CDS             9178..10104
                     /locus_tag="ACHWN2_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(10160..10591)
                     /gene="osmC"
                     /locus_tag="ACHWN2_003113"
     CDS             complement(10160..10591)
                     /gene="osmC"
                     /locus_tag="ACHWN2_003113"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR8932548.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2615 a         2647 c         2709 g         2774 t
ORIGIN      
        1 taaataacat taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg
       61 ataaatccta cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag
      121 caagtaacgg atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc
      181 actatcgcag aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag
      241 attatcaatg atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc
      301 cagacctggg acgacgaaaa cgtccacaag ttgatggatt tatccattaa caaaaactgg
      361 atcgacaaag aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaacatggtt
      421 gccgatctgt ggcatgcgcc tgcgccgaaa aatggtcagg ccgttggcac caacaccatt
      481 ggttcttccg aggcctgtat gctcggcggg atggcgatga aatggcgttg gcgcaagcgt
      541 atggaagctg caggcaaacc aacggataaa ccaaacctgg tgtgcggtcc ggtgcaaatc
      601 tgctggcata aattcgcccg ctactgggat gtggagctgc gtgagatccc tatgcgcccc
      661 ggtcagttgt ttatggaccc gaaacgcatg attgaagcct gcgacgaaaa taccatcggc
      721 gtggtgccga ctttcggcgt gacctacacc ggtaactatg agttcccgca accgctgcac
      781 gatgcactgg ataaattcca ggccgacacc ggtatcgaca tcgacatgca catcgacgcc
      841 gccagcggtg gctttctggc accgttcgtc gccccggata tcgtctggga cttccgcctg
      901 ccgcgtgtga aatcgatcag tgcttcaggc cataaattcg gtctggctcc gctgggctgc
      961 ggctgggtta tctggcgtga cgaagaagcg ctgccgcagg aactggtgtt caacgttgac
     1021 tacctcggtg gtcagattgg tacttttgcc atcaacttct cccgcccggc gggtcaggtg
     1081 attgcacagt actatgaatt cctgcgcctt ggtcgtgaag gctataccaa agtacagaat
     1141 gcttcctacc aggtcgctgc ctatctggcg gatgagatcg ccaaactggg gccgtatgag
     1201 ttcatctgta ccggtcgccc ggacgaaggc atcccggcgg tttgcttcaa actgaaagat
     1261 ggtgaagatc cgggatacac cctgtacgac ctctctgaac gtctgcgtct gcgcggctgg
     1321 caggttccgg ccttcactct cggcggtgaa gccaccgaca tcgtggtgat gcgcattatg
     1381 tgtcgtcgcg gcttcgaaat ggactttgct gaactgttgc tggaagacta caaagcctcc
     1441 ctgaaatatc tcagcgatca cccgaaactg cagggtattg cccagcagaa cagctttaaa
     1501 catacctgat aacgtttaac ggtaacggtg tcccgaaacg aacccgtttc gggataattt
     1561 ccaaagtctg ttcactggca ttagcaacgg aaaatattgt tctgaatacg cttcagaaca
     1621 aaacaggtgc ggttccgaca ggaataccgt tttaggggga taatatggct acatcagtac
     1681 agacaggtaa agctaagcag ctcacattac tcggattctt tgccataacg gcatcgatgg
     1741 taatggctgt ttatgaatac cctaccttcg caacatcggg cttttcatta gtcttcttcc
     1801 tgctattagg cgggatttta tggtttattc ccgtgggact ttgtgctgcg gaaatggcca
     1861 ccgtcgacgg ctgggaagaa ggtggtgtct tcgcctgggt atcaaatact ctgggtccga
     1921 gatggggatt tgcagcgatc tcatttggct atctgcaaat cgccattggt tttattccga
     1981 tgctctattt cgtgttaggg gcactctcct acatcctgaa atggccagcg ctgaatgaag
     2041 accccattac caaaactatt gcagcactca tcattctttg ggcgctggca ttaacgcagt
     2101 ttggtggcac gaaatacacg gcgcgaattg ctaaagttgg cttcttcgcc ggtatcctgt
     2161 tacctgcatt tattttgatc gcattagcgg ctatttacct gcactccggt gcccccgttg
     2221 ctatcgaaat ggattcgaag accttcttcc ctgacttctc taaagtgggc acactggttg
     2281 tatttgttgc cttcattttg agttatatgg gcgtagaagc ttccgcaact cacgtcaatg
     2341 aaatgagcaa ccccgggcgc gactatccac tggctatgtt actgctgatg gtggcggcaa
     2401 tctgcttaag ctctgttggc ggtttgtcta ttgcgatggt cattccgggt aatgaaatca
     2461 acctctccgc aggggtaatg caaaccttta ccgttctgat gtcccatgtg gcaccggaaa
     2521 ttgagtggac ggttcgcgtg atctccgcac tgctgttgct gggtgttctg gcggaaatcg
     2581 cctcctggat tgttggtcct tctcgcggga tgtatgtcac agcgcagaaa aacctgctgc
     2641 cagcggcatt cgctaaaatg aacaaaaatg gcgtaccggt aacgctggtc atttcgcagc
     2701 tggtgattac ttctatcgcg ttgatcatcc tcaccaatac cggtggcggt aacaacatgt
     2761 ccttcctgat cgcactggcg ctgacggtgg tgatttatct gtgtgcttat ttcatgctgt
     2821 ttattggcta cattgtgttg gttcttaaac atcctgactt aaaacgcaca tttaatatcc
     2881 ctggtggtaa aggggtgaaa ctggtcgtgg caattgtcgg tctgctgact tcaattatgg
     2941 cgtttattgt ttccttcctg ccgccggata acatccaggg tgattctacc gatatgtatg
     3001 ttgaattact ggttgttagt ttcctggtgg tacttgccct gccctttatt ctctatgctg
     3061 ttcatgatcg taaaggcaaa gcgaataccg gcgtcactct ggagccaatc aacagtcaga
     3121 acgcaccaaa aggtcacttc ttcctgcacc cgcgtgcacg ttcaccacac tatattgtga
     3181 tgaatgacaa gaaacactaa ccatgtctga atggttataa gacaagggag cgataattca
     3241 tcgctccctt tttcgtgctt gctgtcgtat tgacttaacc gggataagta cgagaatgag
     3301 cgcacatctg tttaccggaa accagcacat atggatatct gctcccgaaa caagaaatta
     3361 acgattagaa gaccagcgat actggttgca ttggcacttt tactgtgtag ttgtaaaagc
     3421 acgcctccag agtccatggt gacaccacca gcaggttcaa agccaccagc cacgacgcaa
     3481 caatcgtcac aaccgatgcg tggcatctgg ctggccacgg tttctcgcct cgactggcca
     3541 ccggtttcct cggttaacat tagtaatccc accagccgag cccgtgtaca acaacaggcg
     3601 atgatcgaca aactggatca tctccaacgt ctcggcataa acacggtctt tttccaggtc
     3661 aagccggacg gtaccgccct gtggccatcg aaaattttgc cgtggtccga tcttatgacc
     3721 ggtaagattg gtgaaaatcc gggttacgat ccgctgcaat tcatgctcga cgaagcccac
     3781 aagcgtggga tgaaagtaca cgcctggttt aacccctatc gcgtatcggt taatacgaag
     3841 cccggtacta tcagggaact gaatagcact ctgtctcaac aaccggcgag cgtctatgtg
     3901 caacaccgtg actggatcag aacgtctggc gatcgctttg tcctcgaccc gggcatccct
     3961 gaggttcagg actggatcac atcaatagtc gctgaagtgg tttcccgcta tccggtagat
     4021 ggcgtgcagt ttgacgacta tttctatact gaatcaccgg gttcacggct aaatgataac
     4081 gaaacgtacc gtaaatacgg aggcgcattt gcgtcaaaag cagactggcg gcgcaacaat
     4141 actcagcagt taattgcaaa ggtatcacac accattaaaa gcattaagcc gggagtcgaa
     4201 tttggcgtta gcccggcagg cgtgtggcgt aaccgatcac acgatccgct cggttccgat
     4261 acccgaggcg cggcagccta tgacgaatcc tacgcagaca cccgtcgatg ggtggaacaa
     4321 ggattgctgg attacattgc tccccaaatt tactggccgt tctcacggag tgccgcgcgt
     4381 tatgacgtgt tggcaaaatg gtgggcggat gtcgttaaac caaccaggac ccgcctgtat
     4441 atcggtatcg ccttctataa agtgggtgaa ccttcaaaga tagagccaga ctggatgatt
     4501 aacggcggcg taccggaact gaaaaagcag ctcgatctta acgatgcggt accagaaatt
     4561 agcggcacca tcttgttccg tgaggactat ctgaataaac cgcagactca acaagcggtc
     4621 agctatctgc aaagtcgttg gggcagttaa actaccatgc ttactttgta acaagccggt
     4681 gctttaccca ccggcttgtt acaccttgtg aaatatccca ctagtaatca tgcttacata
     4741 agtcaaatta accacgcaat taaccacact tagttaaaca gctataaatc cctaataaaa
     4801 atgtatgaat atgaaaaacc atacgatatt cgctgtcacc gaaaaataat tacgatgggc
     4861 tatactcttc tgaagaacag tagcgtagat tgaaactgtc ttaagtcgct tatttctttt
     4921 cagtatatct tcatatttca ggagaatatg gtgcagttag tcgatctcgc acgttgcgtt
     4981 tcgttttgat tccgtctgat ttcccagcct tataagggtg tgaattatgg agatgtattt
     5041 taaaagaatg aaagatgagt ggaccggact tgtcgaacag gcagatcctc tcattcgtgc
     5101 taaagccgca gaaattgccc ttgcgcatgc tcattatctg agtatcgagt tttatcgaat
     5161 tgtccgcatc gacccgcatg ccgaagaatt cttgagtaat gaacaagttg agcggcagtt
     5221 gaaaagtgcg atggaacgct ggattattaa cgtgctttct gcccaggttg acgatgtcga
     5281 aaggctaata caaatccagc ataccgtcgc ggaagtgcat gcccgcatag gaattccggt
     5341 agaaattgtc gagatggggt ttcgggtgct gaaaaaaatc ctctatccgg taatcttctc
     5401 ttcggattat tccgccgcag aaaaacttca ggtctaccat ttctcgatta acagtattga
     5461 tatcgcgatg gaagtgatga cccgcgcgtt tacctttagt gacagtagtg cctcaaagga
     5521 agatgaaaac tatcgtatct tctcgttact ggaaaacgcc gaagaagaaa aagaacggca
     5581 aatagcctca atactttcat gggaaataga tattatctat aaagtcctgc tggattctga
     5641 tttaggcagc agtttgcctt taagccaggc tgattttggc ctgtggttta accataaagg
     5701 tcgacattat tttagcggca ttgccgaagt gggccatatc tcccgtctga ttcaggattt
     5761 cgacggtatt ttcaatcaaa ccatgcgtaa caccaggatt ttgaataaca gaagtttgcg
     5821 ggtgaaattt ctattacaga taagaaatac cgtatcgcag attattacct tgctgcgtga
     5881 attgtttgaa gaagtatcgc gccacgaagt cggtatggat gtactgacga aattacttaa
     5941 ccgccgtttc ctgccgacta tcttcaaacg cgaaattgcc catgccaacc ggaccggtac
     6001 accgctgtca gtgctgatta ttgacgttga taaattcaaa gagatcaacg atacgtgggg
     6061 ccataacact ggcgatgaaa ttctgcgtaa agtctcattc ctctcgcaga agagattggt
     6121 gaaatcgaaa atattgggcg ctgggtcatc gcggaagctt gccgtcagtt agcagaatgg
     6181 cgtagccaga atattcatat cccggcgtta tccgtgaact tgtcggcgct gcactttcgc
     6241 agtaatcagc tgcctaatca ggtgtctgat gcaatgcaag cctggggtat tgacggccac
     6301 cagctgacgg tggaaatcac ggaaagcatg atgatggaac acgataccga aatctttaag
     6361 cgcattcaga tcctgcgtga tatgggcgtg ggtttatcgg tagatgattt tggcacgggc
     6421 ttttccggat tatcccgctt agtcagtctt ccggtaacgg aaatcaaaat tgacaaaagc
     6481 tttgtcgatc gttgtctgac ggaaaaacgc atcctggcct tacttgaagc cattaccagc
     6541 attgggcaaa gcctcaattt aaccgtcgtg gcggaagggg tcgaaaccaa agagcaattt
     6601 gagatgctac gtaagatcca ctgtcgcgtt attcagggat atttcttttc ccgccccctt
     6661 cccgccgaag aaattccagg ctggatgagc agcgtgttac cgctgaaaat ctgacaaatt
     6721 cctctcgccc gcactcgcgg gttttccttt aaggtgacac tgtcacttaa atactgtgat
     6781 ttcagctacg attccgggag attccttcct taactttccc tcttgcacca aaactgcact
     6841 acgctgcaca aatgttgatc aaaagttaaa aaatataact ttaagccagc attctggcgc
     6901 ttatcccggc atggcatgag atctgcataa gcggaaagcg cagcaatttt tgtcttatac
     6961 aggaaccgtt tatgtcggat accaccgaac tggttgattt agccgtgatc ttccctgatc
     7021 tggagatcga attgaaatat gcctgcgttg ataacatcac aggtaaagct atttatcagc
     7081 aagcgcgttg tctgctacat aaagatgcga ttaccgcgct ggcgaaaagt atcagtatcg
     7141 cccagctgtc aggcttacaa ctggtgattt acgatgcgta tcgcccacaa caagcacagg
     7201 cgatgttgtg gcaagcctgc ccagacccgc aatatgttgt tgatgtgacg gtcggttcta
     7261 atcacagccg tggcacggcg ctcgacctga cgcttcgtga tgacgtggag gtggtgaatg
     7321 atgctaagcg aggaaacgtc cgccgtacgc ccacaaaaac aaacgcgatt taacggtgca
     7381 aaactggtat ggatgctgaa aggcagtccg ctcaccgtga ccggcgcagt catcattgta
     7441 ttaatgcgac tgatgatgat tttttcaccg tggctggcga cgcatgatcc caacgccatt
     7501 gatttaaccg cccgcctttt gccgccttct gcggcgcact ggtttggcac cgatgaagtg
     7561 ggacgcgatc tgtttagccg cgtactggtc ggcagtcagc aatcaattct cgccggatta
     7621 gtggtggtcg ccattgcggg tatgattggt tcgctactcg gatgtctatc cggtgtgctt
     7681 ggcggacgcg cagacgccat catcatgcgc atcatggaca ttatgctgtc gattccttcg
     7741 ttggtactga caatggcact ggctgccgct ctcgggccga gtttatttaa cgccatgctg
     7801 gcgattgcta ttgtgcgaat tcccttttat gtgcgcttgg cgcgggggca agcattagtt
     7861 gtacgccagt atacctatgt tcaggcggcg aaaacctttg gcgcgtctcg ttggcatctg
     7921 atcaactggc atattttacg taactcccta ccgccgctga tcgtgcaggc atcgctggat
     7981 atcggtagcg cgattttaat ggccgccacg ttgggattta ttggcctggg tgctcaacaa
     8041 ccgagtgctg aatggggcgc gatggtagcg aatggtcgca actatgtact cgatcagtgg
     8101 tggtattgcg catttccggg ggcagcgatt ttgcttacct ccgtcgggtt taatctcttt
     8161 ggtgatggta ttcgcgatct gcttgacccg aaagcaggag gaaagcagtc atgacccaac
     8221 ccgttctgga cattcaacag ctgcatttga gtttccccgg ttttaacggt gatgttcacg
     8281 cgctcaacaa tgtgtccttg cagattaacc gcggcgaaat tgtcggtctg gtgggagaat
     8341 ccggctcagg taaatcagtc accgcaatgc tgattatgcg tctgctaccg acgggcagtt
     8401 attgcgtaca tcgggggcag atttcactgc tgggagaaga tgttcttaac gctcgggaaa
     8461 aacagcttcg tcagtggcgc ggcgcacgag tggcgatgat ctttcaggaa ccgatgaccg
     8521 cccttaatcc gacacgtcga ataggtcttc agatgatgga cgtaatccgc catcatcaac
     8581 caataagtcg tcgggaagcc agagctaaag cgattgcact gctggaagag atgcaaatcc
     8641 cggatgccgt ggaagttatg tcgcgctatc cgtttgagct ttcaggtggt atgcgccagc
     8701 gggtaatgat tgcgccggca ttctcctgcg agccgcaatt gattattgcc ggcgaaccga
     8761 cgacggcgct ggacgtcaca gtacagttgc aggtactgcg tttgcttaaa cataaagcac
     8821 gcgccagtgg aactgcggta ctgttcatca gccatgatat ggccgtggtg tcgcaactgt
     8881 gcgatagcgt ttacgtgatg tatgccggaa gcgtaattga aagcggcggg acggcagacg
     8941 ttatccatca tccccggcat ccgtatacca ttggtttgct gcaatgcgca ccagaacatg
     9001 gagtaccacg ccagccatta cccgccattc cagggacggt accaaacctc acccatttgc
     9061 ctgacggctg cgcttttcgc gatcgttgct atgcggcagg tgcacagtgt gcgctgacag
     9121 cgtgtggtga caacaaccag cactgcacct gttggtatcc gcagcaggag gtcataagtg
     9181 tctgacacgt tattaacgtt acgcgacgtc catatcaatt ttccggcccg taaaaactgg
     9241 cttggcaaat ctacggaaca tgttcatgcc attaatggta ttgatttaca gatccgccgt
     9301 ggtgaaacct tagggatcgt cggcgagtca ggctgcggca aaagcaccct cgcacagctt
     9361 ttaatgggca tgctgcaacc gagccacggg cagtacatcc gttcaggctc acaacgcatt
     9421 atgcagatgg tgtttcagga cccgctctct tcgcttaatc cgcgcttacc ggtgtggcgc
     9481 atcatcacag aatcgctctg gatagctaag cgtagtagtg aacaacagcg gcgagcgttg
     9541 gcagaggagt tggctgtgca ggtgggtatt cgtccggagt atctcgaccg cctgcctcat
     9601 gcgttctccg gcgggcagcg gcaacgcatc gccattgcca gagcactctc ttcgcagcct
     9661 gacgtgattg tgcttgatga gccaacctcg gcgctggata tctccgtgca ggcgcagatc
     9721 ctcaatttgc tggtaacgct acaggaaaat cacgggctga cttatgtgct gatttcacac
     9781 aatgtctcgg tgatacgtca tatgagcgat cgggtggcgg tgatgtatct cgggcagatt
     9841 gtagaactgg gcgacgcgca acaggtgttg actgcttcgg cacatccata cacccgatta
     9901 ttgctggatt ccctccccgc cattgataaa ccgctggagg aagaatgagc attacgtaaa
     9961 acggatctgc cgggaaaccg cacgttgccg caaggctgtt ttttctacga acgttgccct
    10021 ctggcaaccc acggatgtga agtccggcaa tcattagcaa taagggagga cggacgtgac
    10081 ctccggtgct ggcgggcgct gtagagatgc taacaacgca tcaggcattc acgcgcctga
    10141 cgcgtcatcc ggcaatgctt tacgatttca actggtaatc cagcgtaatt tccgctttca
    10201 gtacctgaga gaccgggcat cctgcttttg ctttctggat gatgccgtca aaggtagagg
    10261 catcaatacc cggcaccgca acttcactct tcagtgcgat tttcgtaatc gcaaaaccgg
    10321 catccacttt atccagcgac acatcggcgg tggtatcaat tgatgttggc gtgaatcccg
    10381 cttcccccag cattaatgaa agcgccattg agaaacatgc ggcatgcgct gcgccaatca
    10441 gttcttcagg gttggttcct ttttcgcctt caaaacgcgt gttaaatcca tacggctgtt
    10501 ggttcagcac gccgctctcg gtggatactg ttcccttccc gcgtttgata tcgccttccc
    10561 agtgtgcctg acctttctta tggattgtca ttgttgctct cctgtgggct taaaaaggaa
    10621 attatagact acgtgagaaa cagcacgagg ataagcagga tattccgaat aaaatacggc
    10681 gaaaatatta cttctgcact taaattaaat tcccacaata gataaccggg aatatatatc
    10741 tcaag
//
LOCUS       DAZSNO010000129        10659 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZSNO010000129 DAZSNO010000000
VERSION     DAZSNO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10659
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.129"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1803)
                     /gene="selB"
                     /locus_tag="ACHWN2_003114"
     CDS             complement(<1..1803)
                     /gene="selB"
                     /locus_tag="ACHWN2_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR8932549.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGN"
     gene            complement(1800..3191)
                     /gene="selA"
                     /locus_tag="ACHWN2_003115"
     CDS             complement(1800..3191)
                     /gene="selA"
                     /locus_tag="ACHWN2_003115"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR8932550.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3289..3897)
                     /locus_tag="ACHWN2_003116"
     CDS             complement(3289..3897)
                     /locus_tag="ACHWN2_003116"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR8932551.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3968..5053)
                     /locus_tag="ACHWN2_003117"
     CDS             complement(3968..5053)
                     /locus_tag="ACHWN2_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR8932552.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5056..5418)
                     /locus_tag="ACHWN2_003118"
     CDS             complement(5056..5418)
                     /locus_tag="ACHWN2_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR8932553.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5955..7868
                     /gene="mtlA"
                     /locus_tag="ACHWN2_003119"
     CDS             5955..7868
                     /gene="mtlA"
                     /locus_tag="ACHWN2_003119"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR8932554.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            7963..9111
                     /gene="mtlD"
                     /locus_tag="ACHWN2_003120"
     CDS             7963..9111
                     /gene="mtlD"
                     /locus_tag="ACHWN2_003120"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR8932555.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9111..9698
                     /gene="mtlR"
                     /locus_tag="ACHWN2_003121"
     CDS             9111..9698
                     /gene="mtlR"
                     /locus_tag="ACHWN2_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR8932556.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9710..9919)
                     /gene="yibT"
                     /locus_tag="ACHWN2_003122"
     CDS             complement(9710..9919)
                     /gene="yibT"
                     /locus_tag="ACHWN2_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR8932557.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10203..10565
                     /locus_tag="ACHWN2_003123"
     CDS             10203..10565
                     /locus_tag="ACHWN2_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR8932558.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         2557 a         2881 c         2751 g         2470 t
ORIGIN      
        1 atttccacga cgacgcgtaa agccaatgcg gtcaaaatat tccagaatct gaattgccag
       61 ctttcggcct acgcctaagc gatcgcggaa atccgccgcg caggttgaac cacactcctg
      121 atcgagatcg cggatcatat tggcaaattc gacaatccga tcgttacggt aataacgatc
      181 tttgacgatc gcggtaatta ttccttgctg cgccgcctgg cgtagcgtca ggcgcattgc
      241 ctgctcgtcg gtacccgtct cttttgccag atcacgcacc caccacggct cgtcaccaaa
      301 cagcggctct gctttttgcc agatggcctg ctgctcttcg ctgaagcccg ctttgtgatc
      361 cggcagatgc agccaaccgt gatgactgag gatgtcgccg ctttcgcgca tcttttcaat
      421 cagcaacagt actagcgctt catcttccat tggcaacgcc atacgtcgca gacgttcgcg
      481 cccaggccca ggttcatcgc gatgttgctc atgataagtc gctaatgtgt cgagaatttt
      541 ccgttgccag cgagcggcaa ccggcgcatt caacaagcta taaccagcct gaatatagcc
      601 aggctgttgc agcagttcgc gcatcccttc gccgttgagc tggcgcgccc aagcgaaatc
      661 aggaagatta accgcgccgc gttgcaggtg aacggctaac gcttcgctat cattctgtgc
      721 cctggcgagg gaagccagcc attgcagata ctctggttta cgtttaccgc gacgcggcgg
      781 gttaagcatc acgacgcgcg ctccggccag cgtgttgcgg gcagagatat cgcgcaatac
      841 caggcggtcg ttatctgcca gccataacgg ggtgtcgaag accagttcag caaggttatc
      901 ttccagcagt gaaacgcgtc ccgtgacgtg gctggcggcg tggtgaatat gcagcggctg
      961 ccactgggtc agcggtgcat gggtttgaag ctcgacaatc acccgtgtga acggctctgg
     1021 cggcacatcg gcaagcagcc agtcgccacg gttaatctgc tctttttccg catcacccgc
     1081 gatgtcaagc gcgatacgct gcccggcatt ggcggtttct gttggctggt tttgcgcatg
     1141 cagcgcacgt acacgcatcg gtttatttac accagtcagc cagagtgaat cgcctacctt
     1201 cacttccccg cttaacgccg taccggtgac gaccagcccg gcacctttta cggtaaatgc
     1261 gcggtcaatc gcgaggcgga aactatgttg gctggcgtgc tcgcgttccg gcaactgaag
     1321 cagatgctcg cgcagggcat caattccccg accttcggtt gctgcggtga taaacagttt
     1381 tgcctcagca aaaccgtatt cccgcagaac ctcctttacc tggcgttcaa cctcatcaac
     1441 acgcgcttcg tccacgcgat cggctttggt cagcgccact gtcagcatcg ggttaccggt
     1501 cagctgcaaa atcgccagat gctcacgggt ctgtgccatc acgccgtcat cgcacgccac
     1561 caccaacagc gcgtgatcga taccaccaac gcccgccagc atgttggaaa gaaacttttc
     1621 atgaccggga acgtcgataa aaccaggcac gcgaccatcc ggctgcggcc agtaggcata
     1681 gccgagatcg atggtcatgc cgcgcttttt ttcttccggc agacggtcag catttacgcc
     1741 agtaatcgcc tgcaataagg ttgttttgcc gtggtcaacg tgtccggcag tcgcaataat
     1801 catttcaaca acatctccaa aaaccgttgc tcatcttcaa ggcagcgtaa atccagccac
     1861 aatcgtccgt cataaatacg accaatcacc ggcactggca attcacgcca gcgggcggct
     1921 aatgactcaa ggtggctacc gcgtccatca tggggtgtaa acgttaatgc cgcgctcggc
     1981 aggcgatcaa ccggcagcga accactgcca atctgcgaaa gacatggcat aacctgtacc
     2041 gcaaactccg cgccgtaatg tgcgacaagg ggagcctgta aacgttgtgc ctggatttga
     2101 atgacctctg cgctgcgggt aagcaggcgc agggtcggta atttttcact cagagcttca
     2161 gggtgtaaat aaagacgcaa cgtggcttcc agcgccgcga gggtcatttt atccgcgcgt
     2221 aatgcacgct tcagcgggtg gctttgcagg cgggcgatca tctctttttt accaacgata
     2281 atcccggcct gcggcccgcc taacaacttg tcgccggaga agctcaccag actgacgccc
     2341 gccgcaatca actcctgcgg cattggctct ttcggcaaac cgtactggct aagatcgacc
     2401 agcgagccac tgcctaaatc agtcaccact ggaacatcca gctctttgcc gagcgccacc
     2461 agttcagctt cgtctatcgc tttggtgaac ccctgaatgc tgtagttact ggtatgtact
     2521 ttcatcaaca gtgcggtatt ttcattcacc gcctgacgat aatcattcgc gtgcgtgcgg
     2581 ttggtggtcc ctacttcgtg tagggtgcag cctgcctgac gcataacatc gggaatacga
     2641 aacgcgccgc caatctccac cagttcgccg cgagatacca ccacctcttt cccgctggca
     2701 gtggccgcca acatcaataa caccgccgcc gcattgttat tgacgataca ggcatcttcc
     2761 gcccccgtaa tacggcacag cagctgcgcc agcgcccgat cgcgatgtcc gcgtccggcg
     2821 tcgtccagat cgtactcgag ggtcactggc gaacgcatag cctgcgcaac ggcttccacc
     2881 gcggcttccg cctgtaaagc tcgcccaagg ttggtatgca gcacggttcc cgtcaggttg
     2941 atcaccggac gcagcgcgct ctgcgcctct ttcgtcaacc gggcatcgac ttcttgcgcc
     3001 cagttttcac accacgcagg cagcgtctgg ctgccacgaa tcacttctcg cgcttcgtcg
     3061 agcatctgac gcaacaattc caccacgcgg gtgtgaccat aagtatcacg caaagaaagg
     3121 aaggagctat cgcgcaataa gcgatcaata gccggaagtt gactatagag ggaacgcgtt
     3181 tcggttgtca taggaaacct ggctgatcaa ggccctctca cacggagaag ggcgtttaac
     3241 ataaccacgg attgtaacgt gagatgggtc aggaggacat atcgcgcatc aagcctttgg
     3301 cggttcggtg cgagcaaaac tttcgcggct aaacaggttt tcgaccagtt tgactaagtg
     3361 tgggcggtcg acacaccaac ctggcgcaac gcggcggaaa ttgagatatc cgacagcaca
     3421 ggcaatggcg atagtcgcca gattgaccgt atcggttttg agtgtgccat cgaccagata
     3481 tccttccagc acatccagac tgcggttgat tttctcccgc tggcgtaaca attcatcttc
     3541 agactgctgc gccgccggac gcgcctgttc acgcaccgat accagcccgg catccataat
     3601 gccatccgcc agcgcctcaa ttttgcgcac ccgcaacgac tccagcggat cgcgcggcaa
     3661 catcgccgga gcgacattca ttaactcaat atattcagcg atgatcggcg aatcaaacca
     3721 gcattcgccc tcttcggtca ccagcaccgg cactttccct aacgggttaa attgcgccac
     3781 gccgttatcc gcgttatagg gcagttcatt aataaattcg aaagttatgc ccttttctaa
     3841 caacagaata aaaagtttgc gtacaaacgg gctggtgtag ctaccgacga gtttcatgcc
     3901 gagtcctttg tgcgaggaaa aatatcagta tggctcaggc tcacgcaaaa cacaggtgat
     3961 ataagtctca atgatccaaa taaagataat gcatccaact ggtcattctt agcagcactt
     4021 tccgcatcac cgaaacatgg ctgaaatggt cggagtaaac cgccacctgg gcgtagatgc
     4081 cgtcgggtaa ggcatcgata tcatcgttag ggtccagttc aatggttccc agcacaccgt
     4141 ccgtgccggg aacgaccgtt aatgattgca ataccccctg cgcctgataa gaaccgcctg
     4201 gcacgacagg taaaatacta gtcaatttgc cgtggaacac ctgcccaggt agcgcgttaa
     4261 acaccacttc cgcatcatcg ccaggtttca gacgtaacag cgagttttgc cgaaattggg
     4321 cgacaatttg ccgtttttgc tcggggatga agaccatcac cggacgcagc ggcaaggcag
     4381 ctgcgtatgt acctgggcgg atcagtacct gagtgacgta gccattgctt ggtgcgcgaa
     4441 tgacagtctg ctcaagatta tattttgctt cagtaagttg cgctcttaag ctcacaatct
     4501 gcgattgctc gccgttgacc atactgtcga gctgactctg gatctgcgcc tgctccgcca
     4561 ccgagccttt cactaacgca tcctgcgcga ggaaattttg ccgcgcatcg tcgatgtcac
     4621 gttccgagaa cggattcacc gccgcctggc tgcctttcag gtaacgttga taatttttaa
     4681 acagacggtc gcgctccgct gaaacctggg tggtgttggc ctgcgcttca gtgagctgcg
     4741 cacgcagcgt ctttatatta tgcgtcgccg tcatcaggtc agcctgaagt ctgtcaactc
     4801 gcgcctggta acgaaccggg tcgagcttaa aaagcacctc gcccttttga ataagctgat
     4861 tattcttgtc agtgacttca gtaacaattc ccgtcacctg tggcgtgata gggatcgcta
     4921 tcactgcctt ttgcgcggta aaagtgtaag ggtggttgta gttcatcaac aaaatcaaac
     4981 cactcaccag aaacacgcct cccagcgccg ccgtcgccag cgccacgtaa gttaaaacaa
     5041 tcaatagatc cataattaga tctccgggaa cgtggaatag tcagtctttt tctcggcggc
     5101 gagttgatgc tccagccggg aaattcggtc agaaagtgcg gcgatttccg gttcagtcgc
     5161 tttctcctgc gacgcaacat gtgactgcat accccagcca cgctccggtt gatagagcgt
     5221 cgcccagatc cacagaaacg gccagataac atgcagagta aacaggctca cccagcccgc
     5281 cgtatgaata gcgtcggcat gggggtgatt gcgctttttg gcaatcagat aagggatgtc
     5341 atgtatcgcg atgattccat aaaaaatcac cagaaagacg aagatcagca ctcccaacgc
     5401 gaaatagttt agaaacatat ctgcctcgga ttcacgttta tcagtgttgt tttttggctg
     5461 gcagccagaa gggagtcagg ctgatatttt gacaataatc cgggttcgcg attctcgcca
     5521 taacaccaaa gaataatttt tagaggtgat gagttgctta gttacataac gattgtatga
     5581 cgaaggcata acatgctgta gatcacatca ggtgaacgcc gtaagaaaat atcttgtgat
     5641 tcagatcaca aagattcaac aaaccatcaa aacaaaaatg tgacattact cacatttaaa
     5701 tgccattttt agcgaaaatc accgccttgt ttctttttta tacaggcgtt ttgtgatgag
     5761 cgtcacgtca attacctctc taccccctat atttatgtga ctgatatcac acaaaagccc
     5821 gtcgactgga cagttaaccg attcagtgcc agatttcgca gtatctacaa ggtccggcta
     5881 cctctgccgc cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca gcaaacataa
     5941 gaaggggtgt ttttatgtca tccgatatta agatcaaagt gcaaagcttt ggtcgtttcc
     6001 tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc atcaccgcgt
     6061 tatttattcc aacagggtgg ttaccgaacg agacgctggc gaagctggtc gggccgatga
     6121 tcacttatct cctaccgctg ctgatcggtt ataccggtgg taagctggta ggcggcaaac
     6181 gtggtggcgt agtcggtgcc attaccacca tgggcgttat cgtcggcgca gacatgccga
     6241 tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg gtgcattaag cacttcgacc
     6301 gctgggtaga cggtaagatc aaatccggtt ttgagatgct ggtgaataac ttctccgcag
     6361 gcatcatcgg gatgatcctc gctattctgg cattcctcgg cattggcccg attgttgaag
     6421 ccctgtccaa aatgctggct gcgggcgtta acttcatggt tgtccatgac atgctgccgc
     6481 tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc attaaccacg
     6541 gtatcttctc gccgctgggt attcagcagt cccatgaact gggtaaatca atcttcttcc
     6601 tgattgaagc taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg ttctttggtc
     6661 gtggtagcgc taaacagtct gcgggcggtg cggcaatcat ccacttcctg ggtggtatcc
     6721 acgaaatcta cttcccgtat gtgctgatga atccgcgtct gatcctcgca gtcatcctcg
     6781 gcggtatgac tggcgtgttc acgctgacta tcctgggcgg tggtctggtt tctccggcat
     6841 ctccgggttc tatccttgct gtactggcga tgacaccaaa aggtgcttat ttcgctaaca
     6901 tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt ttgctgaaaa
     6961 ccagcaaagt gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg caggacatga
     7021 aagctgagtc taaaggcaca tctccgctat ctgcgggcga tgtgactaac gacctgagcc
     7081 acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg ttcaagtgcg atgggcgcag
     7141 gcgtgctgcg taagaaaatt caggatgcag gtctgtcgca gatttcggtc actaacagcg
     7201 cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac ctgaccgaac
     7261 gcgctatgcg ccaggttccg caggcacagc atatttcgct gaccaacttc ctcgacagcg
     7321 gcctgtacac cagcctaacc gaacgtctcg ttgctgccca gcgccatact gaaaacgaag
     7381 tgaaagtgaa agacagtctg aaagacagct ttgacgattc cagtgctaac ctgttcaaac
     7441 tgggcgcgga gaacatcttc ctcggtcgca aagcagcaac caaagaagaa gcgattcgtt
     7501 ttgctggcga gcagctggtg aaaggcggtt acgttgagcc ggaatacgtt caggcgatgc
     7561 tggatcgtga aaaactgacc ccgacttatc tgggtgagtc tatcgcggtg ccacacggta
     7621 cggttgaagc gaaagatcgc gtactgaaaa cgggcgtggt gttctgccag tacccggaag
     7681 gcgtgcgctt cggtgaagaa gaagatgaca ttgcccgtct ggtgattggt attgcagccc
     7741 gtaacaacga gcacattcag gttatcacca gcctgaccaa tgcactggat gatgagtccg
     7801 tcatcgagcg tctggcacac accaccagcg tggatgaagt gctggaactg ctggcaggtc
     7861 gtaagtaatc caatcctacc ctctcctcac ggagagggtt tgggtgaggg aaaagcctca
     7921 ccccagccct ctcgggtaaa aacattgatg aaggttaata ctatgaaagc attacatttt
     7981 ggcgcaggta atatcggtcg tggctttatc ggtaaactgc tggcagacgc gggtatccaa
     8041 ctgacgtttg ccgatgtcaa tcaggtggta cttgatgccc tgaatgcccg tcatagctat
     8101 caggttcatg tggtcggtga aaccgagcag gtagataccg tttccggcgt caatgctgtc
     8161 agcagcattg gtgatgatgt cgttgatctg attgctcagg tcgatttagt cactaccgcc
     8221 gttggcccgg ttgtgctgga acgtattgct ccggctatcg ccaaagggct ggtgaaacgc
     8281 aaagaacaag gtaatgaatc cccgctgaac atcatcgcct gtgaaaacat ggtacgcggc
     8341 accacgcagc tgaaaggtca tgtgatgaac gccctgccgg aagacgccaa agcgtgggta
     8401 gaagagcacg ttggctttgt cgattccgcc gttgaccgca tcgtaccgcc ttcggcttcg
     8461 gcaactaacg atccgctgga agtgacggta gaaactttca gcgaatggat tgtcgataaa
     8521 acgcagttca aaggcgcact gccgaacatc ccaggcatgg agttaaccga caacctgatg
     8581 gcatttgtcg aacgtaaact cttcaccctg aacacgggtc atgctataac cgcgtacctc
     8641 ggaaaactgg ccggtcatca gaccattcgt gacgctattc tcgacgagaa aatccgcgcg
     8701 gtggtaaaag gtgcgatgga agaaagtggt gcggtactga tcaagcgcta cggctttgac
     8761 gcagacaaac atgcggcgta catccagaaa atcctcggtc gttttgagaa cccgtatctg
     8821 aaagatgatg tagagcgcgt aggccgtcag ccgctgcgta aactgagtgc tggcgaccgt
     8881 ctgatcaagc cactgctcgg tacgctggaa tacagccttc cgcacaagaa tctgattcag
     8941 gggattgctg gtgcaatgca cttccgcagt gaagatgatc cgcaggctca ggaactggca
     9001 gcactgatcg ctgacaaagg tccgcaggcg gcgctggcac agatttccgg tcttgatgcc
     9061 aacagcgagg ttgtatccga ggcggtaacc gcttataaag caatgcaata atggtggacc
     9121 aggcgcagga caccctgcgc ccgaataaca gattgtcaga tatgcaggca acaatggaac
     9181 aaacccaggc cgttgaaaac cgtgtgcttg agcgtctgaa tgctggcaaa accgtgcgaa
     9241 gctttctgat caccgccgtc gagcttttga ccgaggcggt aaatcttctg gtgcttcagg
     9301 tattccgcaa agacgattac gcggtgaagt atgctgtaga accgttactc gacggcgacg
     9361 gtccgctggg cgatctttct gtgcgtttaa aactcattta cgggttgggc gtcattaacc
     9421 gccaggaata cgaagatgcg gaactgctga tggcattgcg tgaagagcta aatcacgacg
     9481 gcaacgagta cgcctttacc gacgacgaaa tccttggacc ctttggtgaa ctgcactgcg
     9541 tggcggcgtt accaccgccg ccacagtttg aaccagcaga ctccagtttg tatgcaatgc
     9601 aaattcagcg ctatcaacag gctgtgcgat caacaatggt cctttcactg actgagctga
     9661 tttccaaaat cagcttaaaa aaagcctttc aaaagtaagc aacgtctgct tactgcccct
     9721 ctacctgctt cggccgataa agccgacgat aatactccag acgttgtaga tataacggca
     9781 cgctttcatc gggtattccg gacggaatcg cgttacgggg aggaagtttt ttcagatact
     9841 cccggaacgc ctggcttgat gccatgaaat ctacggcttt atcgataaga agcggaacgt
     9901 tttcacctaa tttgcccatg atgatatctc cgtattaccc cgcgccggga atgcgcggcc
     9961 gccaatttta gtttaggttc ccggtaaaga tgacattaag gaaaacgtgc tgaatcctca
    10021 ataatcatcc gctgctgttt attatctttt tctatagatt tcttaattaa tcaacgaatt
    10081 gcgtagtttt tgagcatgtt aatgattacg ttgcagaact attacaatgt catcagttgt
    10141 gaacaaagca cctggtcgcg catactaggg gctataaatt tatctttatc agaagccatc
    10201 acatgaaaga agtcgaaaaa aacgaaatca agcgtctcag cgatcgcctg gacgccatcc
    10261 gccaccagca ggccgatctg tcgctggttg aagccgcaga caaatatgcc gagctggaaa
    10321 aagagaaagc cacgctggaa gcagaaattg ctcgcctgcg tgaggttcat agccaaaaac
    10381 tgagtaaaga agcacagaag ctgatgaaga tgccgttcca gcgcgcaatt accaaaaaag
    10441 agcaggctga tatgggcaag ctgaagaaaa gtgttcgcgg actggtggtt gtgcacccaa
    10501 tgaccgcgct ggggcgcgaa atgggcctgg aagagatgac tggattttct aagactacgt
    10561 tttaagcccc tcagacgact ggttcgatcg tatcaacccg catctggctt gccttagctg
    10621 gtcaatgcgg ggactctcct gtctactcgc ctaattacc
//
LOCUS       DAZSNO010000130        10645 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZSNO010000130 DAZSNO010000000
VERSION     DAZSNO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.130"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(385..819)
                     /locus_tag="ACHWN2_003124"
     CDS             complement(385..819)
                     /locus_tag="ACHWN2_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFR8932559.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(807..1208)
                     /locus_tag="ACHWN2_003125"
     CDS             complement(807..1208)
                     /locus_tag="ACHWN2_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR8932560.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(1468..2037)
                     /locus_tag="ACHWN2_003126"
     CDS             complement(1468..2037)
                     /locus_tag="ACHWN2_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFR8932561.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            2236..2436
                     /locus_tag="ACHWN2_003127"
     CDS             2236..2436
                     /locus_tag="ACHWN2_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932562.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVCILCSW"
     gene            complement(2782..2925)
                     /locus_tag="ACHWN2_003128"
                     /pseudo
     CDS             complement(2782..2925)
                     /locus_tag="ACHWN2_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
     gene            complement(3259..3525)
                     /locus_tag="ACHWN2_003129"
     CDS             complement(3259..3525)
                     /locus_tag="ACHWN2_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932563.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            3666..3872
                     /locus_tag="ACHWN2_003130"
     CDS             3666..3872
                     /locus_tag="ACHWN2_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8932564.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            3967..4569
                     /locus_tag="ACHWN2_003131"
     CDS             3967..4569
                     /locus_tag="ACHWN2_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932565.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            complement(4475..4671)
                     /locus_tag="ACHWN2_003132"
                     /pseudo
     CDS             complement(4475..4671)
                     /locus_tag="ACHWN2_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333284.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5769..6686
                     /locus_tag="ACHWN2_003133"
     CDS             5769..6686
                     /locus_tag="ACHWN2_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932566.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            6771..7643
                     /locus_tag="ACHWN2_003134"
     CDS             6771..7643
                     /locus_tag="ACHWN2_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFR8932567.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            8015..>10645
                     /locus_tag="ACHWN2_003135"
     CDS             8015..>10645
                     /locus_tag="ACHWN2_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFR8932568.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFS"
BASE COUNT         2559 a         2774 c         2749 g         2563 t
ORIGIN      
        1 tcgcctgccg ggagtcacat ttttctgcat atctgacact ttggttccca ctacttattt
       61 ggtggacacc actttgtcta attcgtcaga ttctgaccag acggttcagg ctgtacgctt
      121 accactattt tatccctgtt gaatttggtt aagccggtgc agacgcgccg ggaaacgcac
      181 acctgaaccg gcacaataaa atacaataca accccctgtt actggagata cgaatggaat
      241 gacgaggcag cctgtcagtc acagggggct gttggcataa aatcaacgga aaggccacca
      301 acaccggaat taagctccac agtccccgca ctggcaatcc attcccggga aaggcatcac
      361 cgcaaactgc tccggaggca accattacag cctctgtaac ggattatcag ccctggcatc
      421 ttccgacggc acctcctgcc gcccccacgc cctgctgtat accccggtac cgcagcgtgg
      481 gcagcacttt tccccctcat aatcttcgtc acacatcaca caaaaccact ttctcacctt
      541 caccgtataa tccggaaccg tcatgccctg cagatgcaaa cgacgggcct ctttctctgc
      601 cgggtcaagc caggtacatc gcagcaggat tttttcagcg acagtcatct ggtcatgttc
      661 aaaccacggt ggttgtccgt cattgccccg ctgaagtgtt aacgggcatc cacaggacac
      721 acagcacaaa acgtcctgtc ggttgtgctt cgcagcttct ttcgccgtaa caaaatggcc
      781 tttgctgtca ttcgccatat aacagttcag aattcgcata tttcctcctg agttgtccgg
      841 ggaatactga atcctccggt ctggcagtgt gtgcagtact gctccccata ataatcgtgg
      901 tgacattgtc tgcagtgcca gctggcttta cgcaccacgg gtaaggcatc cggtacgaat
      961 tgctgcagac gtttaatcaa ctgtatttct ctgcgctccg gcctgacata agggcactgt
     1021 tgaccgtgct ctgtcagcct gtcgtcagtg tgttcaaacc agggaagttc agtttcgtat
     1081 tgcggatggt atctgagtgc actgccacac aagtggcagg tgtagttagc ataaggtgcg
     1141 gcctgtgcag tacgggcgcc cgtcagacgt ccgttgccat caagagcgat aaaggatttt
     1201 gcgtacatag tcatatacct cagccgccag acgacacgca ggcaacagcc gtcccggtac
     1261 gggcagcgtg gtcagggtgt gaatggcggt tcagttaaaa tctggactgg agtttgggta
     1321 aagcaggctg gttgctgtta cagcactgtg gttgtcacac aatggaacac tgacgtaaaa
     1381 aacagtcagt gggcgagtgg atacatcgtt gccgccagac aacaacccat acttttggtt
     1441 tgccggatat tggcgcgcca gtcgtgctca actccggcta cagccaaaat tgcgctcacc
     1501 ggtgcagtga gctttggtat gctccttcgc cagatagtca gcacgttcca gcacttgctg
     1561 aaagccggtg tcatcattac gcgccagcca caccgccggc caggccggaa aatgcaccag
     1621 cgtggcatgg cacccgacat ccactcccag ggcactgcac cacgcctgtt taatcatccc
     1681 ggccagagac tccggagcgc ggtaatcacc ggcacgacac caggtatccc ggttgaccag
     1741 cagcatcagg tgatagtgtt ttttaccttt gcactcccca aactcccggg cccagacgta
     1801 atgcagggtt gtgggatgca cgcgtttacc ttcccgatgc ttacgtttct ggtaagcgtc
     1861 gattcgggct ttcagggcat tgatgaagcg ggatatcaca gctgcgtccg ttgctgccgg
     1921 tacatccggg agacgcaggt caacccgcaa tgccgtcagg cggggatgaa cattcagtgc
     1981 gtgccgcacc gtctcacgaa tacgttgctg ccagaagggg ttgtatttgt aggtcatggt
     2041 taaatctccg tatggttcat acggaatagc cacgtcgtaa aaaatacgca gagtccctga
     2101 agtgacctcc ggcaggacac tgtctcaggc actctgtgat aacccgaacg gtcgtctccg
     2161 gactgacggt tgaattttca gcgtcgtttt ttaatgtaaa aaaccggtta cgggtaaggc
     2221 tgtgaggagg gagtgatggt gatacgcaaa aagaagtgca gggactgtgg aaacgcgatt
     2281 actcataata cagtgtgttg cccgtattgt ggttctgtcg accccttcgg ctactaccgg
     2341 aatacagacc gtatcgttac catactgctg gcgctgatta ttgtggtcct gctgactact
     2401 gtcagcgtca gtgtgtgtat actgtgtagc tggtgagata acggttcatg tatcccctga
     2461 gctgacagtg taccgtgtat attaagcgta accgacaggt tctccttcat accatattaa
     2521 attttccagg gattgccgct gccttatttc gtcccataca ggaaaactca tctgggtttc
     2581 tgacaaatac gcgaaactca cagcccctca atatcaacca cacgttgtaa gccggtaata
     2641 cctgaaaatc agcctattac cgcccacgcc tccggcacca ttcccttttg atttcgaatt
     2701 ctttgttgtc gctataccgc ttaatgttcc cggtcctccg gtgcagataa caccggagtc
     2761 atcgtagcgc tgcttcaata ttcaggtgac gtatttccag acggaagccg gcgcgcgatc
     2821 gtaaagtttg ttcattgtca gttctgccag ccggttgtca acggcagagt taaaggcttc
     2881 cagctccgca agggtaagtt tataacgcgt gtgggaattc acttttcccg cgtctcctga
     2941 cgtattacgg gctgccactt acctggtgtc ccgcaataca gccttagtgt tccgttatcg
     3001 gggatccgga cgcaccgcaa caaccgggga agtatttctg gtttagagca gtgtcaagag
     3061 ggatttctca gcccctcact caccggctca gtctcagggc aggagcatta atacgctcag
     3121 ttcatgtctt atacaggaat gggcacaggg ggagctggcg tgtcactgct gattttacgc
     3181 atcagcgttc atgtatctgc actctgatac atgaacgatt cacagcgctc tgttaccgga
     3241 gagatttact ctcttagctt actctggcaa atcctttacg ttactctctg atgacttctt
     3301 atatttataa taccaatcag acagccttat cccatcggtg atatcagtct cctcctcgcc
     3361 aggaacgggc tgactcactg taactacctc cgaaaacaac tctgcaatta tcagaacgcc
     3421 tgcttctctc cctgtcctca cgaaaactat cccctcttta tcgcgcgtgc gtgcggaagc
     3481 atcttttcgc aacaaccacc cggtataccg gtacggctca gccaccgcaa cctccccgtt
     3541 tatctcctgg cggtcacatt cccgattatt ttttacgttt ctccccggtt gttatgccgg
     3601 tgaaggtggc gcgctgtttt catgaccaca ccggctggtt taacaacatc cggaggaaca
     3661 ttctcatggc taccccagtt tcactgatgg atgaccagat ggtcgacatg gcatttatca
     3721 ctcaactgac cggcctgaca gataagtggt tttacaggct catcagggat ggggcctttc
     3781 cggctcccat caagctgggc cgcagctccc gctggctgaa aagtgaagtg gaagcctggc
     3841 tacaggcgcg tattgcacag ccccgcccgt aatttctgac cgttatccgt tcacccgcag
     3901 cagacgtaat ccccggcctg ccggcggcat tctgctgcct gttatatccc cgtgaggaat
     3961 atgaaaatga aacaacagta tcagacccgc tacgaatggc tccacgaaag ctaccagaaa
     4021 tggctgaccg gcttcacccg gcacgccgta tcctggggcg tgtgtcatcc gaatatctac
     4081 tacttccata atctgacgcc cggttgggtg tcattcaatg gtgaaaaacc ggagattgcc
     4141 atcgtacccc agagcctgca ccggctgatt tatggccctg acaaacgggc cacgccgccc
     4201 ctggatgatg atctgatagt gaatttatgc accagtgaac atctgctggt tcatcatccg
     4261 atgctggaag gcattctcct gtctgaatgt gaacgtctga ggcagcgttc tctggcaaat
     4321 aaactgatca gcctcttccg tcagtttggt ggcacggagt tgcgcctcaa actggtctgg
     4381 ctttgctggc ttgatttaat gaccggaaac agccttgaag actggaaaga gaacctgaaa
     4441 cggaaatcag aaaaagagct ggaggaatgg atcatcaacc gtcagaggca gagcgcagca
     4501 ctgacggatc tgatggatca gtacgtgctc ctggcatacc gcacaacggt tgacgatagc
     4561 cgcaactgat gccggttgct tccggaaggt gtgacaaaat acggaaatga tgatgctccc
     4621 ggcccccatg ctcacctgca gagcgtgttc gtcgtcatca tactgagcca tgacgggcgt
     4681 cttaagggca tcctgtcatt gctgagcagg caacaccacc agaggagcca cggacaggga
     4741 tgttctttac agcccgatac acgccaggtt ctcagcagac tgaatgtgca ggtgcggtta
     4801 ttcaccagaa atggcatgct gcggtacata cccatcctgg tgacaatatg tacgtggcga
     4861 cagcgatccc ggcactgtgg aagagactca ctcagcctgc aggatattct ttccggcatc
     4921 cgggaatgtc cgcctgaatg caaacaggat gatatttgaa tccgtaatac tgttctttca
     4981 tcataaataa tttatgcaga tacagttaac cgttacagta gccggtttgt tttaccggga
     5041 aataatatcc gtcagactga taccggaaca tatctgaaac acgttatgat gttttgttat
     5101 cagaaatgtt gtatattctt atccgtggcg acagtttaat gccctctggt atcacctgat
     5161 aatgtatatc ctgcgatgat tcgttatatt tacgggacac gcctcttttc attgtgctcc
     5221 ttttcagact tcattttccg taagtgttcc ccggcataaa ggaggtacct ttctggcgtt
     5281 tataactctc tttatcggtg acgccggtaa tacagggaat accacaccga caaacgaggt
     5341 attaacagac gatatttcag tgtccataat ttttctgtcc ggttaaataa cgtcagtctg
     5401 ctgccctctg actgtaatca tcaaagccgg aatgcatacc gggttacaac gtaaaaaaag
     5461 tgatgctggc ccccggtgca gctggcgggc gaaacatgtt tacctttcaa taccccctgt
     5521 ctgcagtctc cactcttcca tcctcttaag ctacgtcggc cacatgagtg cacttccatg
     5581 ataacaggta ttcctgtcag cataacgaag ccggttacct tcccttcctc ctgcatttcc
     5641 tgtcagaatg actatacagc gcgcagattg cctttttatt gaccatctta atataccgtt
     5701 ataaacttct ttttatcata ttattatcac cttattatgt ttaattttta agcagtcgga
     5761 gaaagaggat ggttcagaaa attttgtcgg ataaggttat gaatgaaaga acaaatgcgt
     5821 attactctta ttatcttgga gagcgtaata tctccgtact gccccttaat gtttatgatc
     5881 ccccggaacg ttttatcgct tacataaaaa aaaacagaga aaatctgaac atcacacttt
     5941 cagattttga acttgaacaa ataatttctg gtatgcgtct gaaggccctg gccttcctgg
     6001 tccccctgga aaaaatatcc tggatcgcgg gcagtgaacg cgcatgctta ttttcctggt
     6061 atctgttgat gcagtttatc cagaataaca gggcaaaaat aagcgcagac ttgcttcaaa
     6121 agaataaact gtatcttaaa gaagagtatc tggaagggaa tgccttcccg tcggactcat
     6181 cgacacagtt cagacaaata ctcagagttc tcgatattct ttctgataag aatttgcggg
     6241 atgagtggat aatccagacc aaagacagat ggatgcgggc atttaaatca aagagtcctt
     6301 tcagttacct cctcccggag aacgaacatg aatgcatatg gacatggaat tatttgaagg
     6361 gaaaaaatat tgcactggaa aaactggcca gttttcctgg ttctgctgat atttaccacg
     6421 caattcacct ttcatttgac atatgggtta cctgtccact cacctcacct gacgatataa
     6481 aaaatttcag aaacagtttc aataaggcca aggcgcagcg caagtacaaa aaaatgcagg
     6541 aagataaagt caatgtacag ttttttcttg acgcagaaac cagggcgcag ctcaaagaat
     6601 tatcgagggt cagaagactc agtaccggcg aaatgctgca tgacctgatt gttgaagagt
     6661 ataaaaggta ccgacattcc agataatttc agacatatat tatctccgtg agtcccccgc
     6721 cacctttccg gtgtgcgggg gttttgtctt ttttcaccgg gaatacatgt atgaatccgt
     6781 ctgatgccat tgaggcaatt gaaaaaccgc tctcctccct gccttactca ctttcccgtc
     6841 acatcctgga acatctgcgc aaactcacca gtcacgaacc cgtgattggc attatgggta
     6901 aaagcggggc cggtaaatcc tcactctgta atgcactgtt tcagggggag gtcaccccgg
     6961 tcagtgatgt tcacgccggc acccgggaag tgcagcgttt ccgtctgagt ggccatggtc
     7021 acagcatggt tatcactgac ctgcccgggg tgggcgagag ccgggacaga gatgcagagt
     7081 atgaagccct gtaccgtgac attctgcctg aacttgacct ggtactgtgg ctgattaaag
     7141 ccgatgaccg tgccctgtct gtggatgagt atttctggcg acacatcctg caccggggac
     7201 atcagcaggt gctgtttgtg gtgacgcagg ccgacaaaac ggagccctgc catgaatggg
     7261 atatggccgg tattcagcct tctcccgcac aggcacagaa tattcgcgaa aaaacggagg
     7321 cggtattccg tctgttccgg cccgtacatc cggttgttgc cgtatcggcc cgcaccggct
     7381 gggaactgga tacgctggtc agtgcgctaa tgacagcact tcccgaccat gccgccagtc
     7441 ccctgatgac ccgcctgcag gacgagctgc gcacggaatc tgtccggggt caggcccgtg
     7501 aacagtttac cggtgcggtg gaccggatat ttgacacggc ggagagcgtc tgtgttgcct
     7561 ctgttgcccg tacggttctg cgtgccgtcc gtgacacggt ggtctctgtt gcccgcgctg
     7621 tatggaactg gattttcttc tgaacagtct gccagggaaa tgcctgataa cattacgtta
     7681 accaactgaa aacatgacac aaacccctca tactcctcct gtttctcttg cgtgaccgcc
     7741 ttgtcgttaa tagaataaaa ctatcgataa aacagatatc gatcgttgct atcgatcgtt
     7801 tatagtggtt aatggtctgc gaaaaggcag ctgaatctct tcatcatgca gaacggaatt
     7861 gcacacaacg gactgatact tctctggcgg atgagagagg ggaagcattt atgtccggga
     7921 aaaaccactt tatctgacca tgctgttttg tacctgccgg tatccacttt tgtgggtacc
     7981 ggctttttta ttcaccctct gtaaggaaaa gctgatgaaa cgacatctga acaccagcta
     8041 caggctggta tggaatcaca ttacgggcac cctggtggtg gcttccgaac tggcccgctc
     8101 acggggaaaa cgcgccggtg tggcggttgt gctgtctctt gctgctgtca catcagtccc
     8161 ggcactggct gctgacacga ttgtacaggc gggagaaacc gtgaacggcg gaacactgac
     8221 aaatcatgac aaccagattg ttctcggtac ggccaacgga atgaccatca gtagcggtct
     8281 ggagtatggg ccggataacg aggccaatac cggcgggcaa tggatacaaa atggcggtat
     8341 cgccaacaac actactgtca ccggtggtgg tcttcagaga gtgaatgccg gaggaagcgt
     8401 ttcagacacg gttatcagtg ccggaggcgg acagagcctt caggggcagg cagtgaatac
     8461 cactctgaac ggcggtgagc agtgggtaca tgaaggcggg attgcaacgg gtaccgtcat
     8521 taatgagaag ggctggcaga ccgtcaaatc cggcgcaatg gcaaccgaca cggttgtgaa
     8581 taccggcgcg gaagggggac cagatgcaga aaatggtgat accgggcagt ttgttcgcgg
     8641 aaatgccgta cgtaccacta tcaatgaaaa tggtcgtcag attgtggctg ctgaaggaac
     8701 agcaaatacc actgtggttt atgccggcgg cgaccagacg gtacacgggc atgcgctgga
     8761 taccacactg aatggcggtt accagtatgt gcacaacggc ggtacggcgt ctggcactgt
     8821 tgtaaacagt gacggctggc agattatcaa ggaaggtggt ctggcggatt tcaccaccgt
     8881 taaccagaaa ggcaaactgc aggtgaacgc cggtggtacc gccacacatg tcaccctgaa
     8941 acagggtgga gcactggtta ccagtactgc ggcaacggtt cttggcagca accgtctggg
     9001 caatttcacg gtggaaaacg gtaaggctga cggagttgtt ctggagtccg gcggtcgcct
     9061 ggatgtactg gaaggccatt cagcgcagaa aacacgggtg gatgacggcg gaaccctggc
     9121 agtgtctgcc ggtggtaagg caacaggtgt caccatgaca tccggtggtg ccctgattgc
     9181 agacagtggt gccactgttg aggggaccaa tgccagcggt aagttcagta ttgatggcac
     9241 atccggtcag gccagcggac tgctgctgga aaatggcggc agctttacgg ttaatgccgg
     9301 aggactggcc agcaacacca ctgtcggaca tcgtggaaca ctgacgctgg ctgccggggg
     9361 aagtctgagt ggcagaacac agctcagtaa aggtgccagc atggtactga atggcgatgt
     9421 ggtcagtacc ggcgatattg ttaacgcagg ggagattcgc tttgataatc agacgacacc
     9481 ggatgccgcg ctgagccgtg ctgttgcaaa aggcgactcc ccggtaacgt tccataaact
     9541 gaccaccagt aacctcaccg gtcagggtgg caccatcaat atgcgtgttc gccttgatgg
     9601 cagcaatacc tctgaccagc tggtgattaa tggtggtcag gcaaccggca aaacctggct
     9661 tgcgtttaca aatgtcggaa acagtaacct cggggtggca acctccggac agggtatccg
     9721 ggttgtggat gcacagaatg gcgccaccac agaagaaggt gcgtttgccc tgagtcgccc
     9781 gcttcaggcc ggcgccttta actacaccct gaaccgtgac agcgatgaag actggtacct
     9841 gcgcagtgaa aatgcttatc gtgctgaagt ccccctgtat acatccatgc tgacacaggc
     9901 aatggactat gaccggattc tggcaggctc ccgcagccat cagaccggtg taaacggtga
     9961 aaataacagc gtccgtctca gcattcaggg cggtcatctc ggtcacgata acaacggcgg
    10021 tattgcccgt ggagccacgc cggaaagcag cggcagctat ggcttcgtcc gtctggaggg
    10081 tgacctgctg agaacagagg ttgccggtat gtctctgacg acaggggtgt atggtgccgc
    10141 aggccattct tccgttgatg ttaaggatga tgacggttcc cgcgccggca cggtccggga
    10201 tgatgccggc agcctgggcg gatacctgaa tctgacacac acgtcctccg gcctgtgggc
    10261 tgacattgtg gcacagggaa cccgccacag catgaaagcg tcatcggaca ataacgactt
    10321 ccgtgcccgg ggctggggct ggctgggctc actggaaacc ggtctgccct tcagtatcac
    10381 tgacaacctg atgctggagc cacaactgca ctatacctgg cagggactta ccctggatga
    10441 cggccaggat aacgccggtt atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc
    10501 cggtttccgt ctgggcagcc acaacgatat gagctttggt gaaggcacct catcccgtga
    10561 cactctgcgc gacagtacaa aacaccgtgt gagtgaactg ccggtgaact ggtgggtaca
    10621 gccttctgtt atccgcactt tcagc
//
LOCUS       DAZSNO010000131        10464 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZSNO010000131 DAZSNO010000000
VERSION     DAZSNO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.131"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="ACHWN2_003136"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="ACHWN2_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR8932569.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="ACHWN2_003137"
     CDS             214..402
                     /locus_tag="ACHWN2_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR8932570.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="ACHWN2_003138"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="ACHWN2_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR8932571.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="ACHWN2_003139"
     CDS             597..770
                     /locus_tag="ACHWN2_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR8932572.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="ACHWN2_003140"
     CDS             complement(819..1892)
                     /locus_tag="ACHWN2_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFR8932573.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4705)
                     /gene="torS"
                     /locus_tag="ACHWN2_003141"
                     /pseudo
     CDS             complement(1994..4705)
                     /gene="torS"
                     /locus_tag="ACHWN2_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="ACHWN2_003142"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="ACHWN2_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR8932574.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="ACHWN2_003143"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="ACHWN2_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR8932575.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="ACHWN2_003144"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="ACHWN2_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR8932576.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDITGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="ACHWN2_003145"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="ACHWN2_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10464
                     /gene="torD"
                     /locus_tag="ACHWN2_003146"
     CDS             10277..>10464
                     /gene="torD"
                     /locus_tag="ACHWN2_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR8932577.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2525 a         2684 c         2788 g         2467 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tgactgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattacagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccac
//
LOCUS       DAZSNO010000132        10456 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZSNO010000132 DAZSNO010000000
VERSION     DAZSNO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10456
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.132"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            179..1573
                     /gene="galP"
                     /locus_tag="ACHWN2_003147"
     CDS             179..1573
                     /gene="galP"
                     /locus_tag="ACHWN2_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR8932578.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1650..2147
                     /locus_tag="ACHWN2_003148"
     CDS             1650..2147
                     /locus_tag="ACHWN2_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR8932579.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            2242..2949
                     /gene="endA"
                     /locus_tag="ACHWN2_003149"
     CDS             2242..2949
                     /gene="endA"
                     /locus_tag="ACHWN2_003149"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR8932580.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            3029..3760
                     /gene="rsmE"
                     /locus_tag="ACHWN2_003150"
     CDS             3029..3760
                     /gene="rsmE"
                     /locus_tag="ACHWN2_003150"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR8932581.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3773..4723
                     /gene="gshB"
                     /locus_tag="ACHWN2_003151"
     CDS             3773..4723
                     /gene="gshB"
                     /locus_tag="ACHWN2_003151"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR8932582.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4832..5395
                     /locus_tag="ACHWN2_003152"
     CDS             4832..5395
                     /locus_tag="ACHWN2_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR8932583.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5395..5811
                     /gene="ruvX"
                     /locus_tag="ACHWN2_003153"
     CDS             5395..5811
                     /gene="ruvX"
                     /locus_tag="ACHWN2_003153"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR8932584.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5987..6967)
                     /locus_tag="ACHWN2_003154"
     CDS             complement(5987..6967)
                     /locus_tag="ACHWN2_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR8932585.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            6985..7689
                     /gene="yggS"
                     /locus_tag="ACHWN2_003155"
     CDS             6985..7689
                     /gene="yggS"
                     /locus_tag="ACHWN2_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR8932586.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7707..8273
                     /gene="yggT"
                     /locus_tag="ACHWN2_003156"
     CDS             7707..8273
                     /gene="yggT"
                     /locus_tag="ACHWN2_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR8932587.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            8270..8560
                     /gene="yggU"
                     /locus_tag="ACHWN2_003157"
     CDS             8270..8560
                     /gene="yggU"
                     /locus_tag="ACHWN2_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR8932588.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8568..9161
                     /locus_tag="ACHWN2_003158"
     CDS             8568..9161
                     /locus_tag="ACHWN2_003158"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR8932589.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9154..10290
                     /gene="hemW"
                     /locus_tag="ACHWN2_003159"
     CDS             9154..10290
                     /gene="hemW"
                     /locus_tag="ACHWN2_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR8932590.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2472 a         2738 c         2740 g         2506 t
ORIGIN      
        1 ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat gcccttaaat
       61 cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct tcacatcttt
      121 ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg agggcatcat
      181 gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg tctgcttcct
      241 tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg gcgcactgcc
      301 gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg taagctccat
      361 gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta aactcgggcg
      421 caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt tctctgcggc
      481 tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg cggtgggtgt
      541 ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa ttcgcggcag
      601 tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt atctttctga
      661 taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta tcatcccggc
      721 aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt ttgccgccaa
      781 acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca gcgcggaagc
      841 gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg gctgggcgct
      901 gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt tacaggtaat
      961 gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct tcgaactggc
     1021 gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc tgaccaacgt
     1081 acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac caacgctaac
     1141 gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga tgcatatcgg
     1201 tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt ttattgtcgg
     1261 ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc agccgctgaa
     1321 aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca acatgatcgt
     1381 tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct tctgggtgta
     1441 tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg aaaccaaaca
     1501 cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc gcgaaatcgg
     1561 cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc tcttgcagtc
     1621 atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct atcgccatcc
     1681 aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg aagctagggc
     1741 gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc ggaacagcct
     1801 ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac agtgaagctt
     1861 ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa catttcggcc
     1921 gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg ggcgttcccg
     1981 cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc ccctaccgct
     2041 gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt cgtggcgagg
     2101 ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca cctgaactat
     2161 caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac gttgctggct
     2221 cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt ggtactgagc
     2281 gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca ggcgaaagcc
     2341 gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg taaaattaac
     2401 tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt gcgcaaaaat
     2461 gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca attcggtcac
     2521 cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc ggtctatcgc
     2581 aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa tggcgatcgc
     2641 ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca atgcgccatg
     2701 aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg tgccattgcg
     2761 cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca gcaaacgcag
     2821 ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga gcgcgatgaa
     2881 cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg ccaggcgcga
     2941 aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg cgtacaaccg
     3001 cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc atcctgaacc
     3061 actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata tcgggcgcgt
     3121 actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc aggtctttga
     3181 cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg aaggccagat
     3241 cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc gtggtgaaaa
     3301 aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta cgccactttt
     3361 ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc ttcagcagtg
     3421 gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc cggaaatccg
     3481 tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga aactgaatct
     3541 tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac gcgtccgcct
     3601 gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg cccgctatca
     3661 atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg cgctcaccgc
     3721 cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa taatgatcaa
     3781 gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt ccagtttcgc
     3841 tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga tgggcgatct
     3901 gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga agcagaacta
     3961 cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc tcgatgtgat
     4021 cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct atattctgga
     4081 acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc gcgactgtaa
     4141 cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg ttacgcgcaa
     4201 taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta agccgctgga
     4261 cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc tcggcgtgat
     4321 tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt acctgccagc
     4381 cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac cgtactgcct
     4441 ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg gtcgcggtga
     4501 acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc cgacgctgaa
     4561 agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga ctgaaattaa
     4621 cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt cgatcaccgg
     4681 aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct tagcgagaag
     4741 gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg gcgctgttgc
     4801 ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact ttcttattgc
     4861 catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt gcgaacataa
     4921 taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca aaattgaagg
     4981 gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc gtctggataa
     5041 accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc atactccgcc
     5101 ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca cttcccgcga
     5161 tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg ctctgggcta
     5221 tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt ggctaacggc
     5281 cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc gcgaggcggc
     5341 aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg cctgatgagt
     5401 ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt cggccaacgc
     5461 attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac gccggactgg
     5521 aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt cggtttgccg
     5581 ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt tgccaaccgt
     5641 attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag cactgtggaa
     5701 gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg caaagttgac
     5761 tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta aggcgatcta
     5821 aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac gccttatcca
     5881 gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca tcagacattt
     5941 attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac aaacgccctt
     6001 cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc tgcccggttt
     6061 gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc accgcgggag
     6121 tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact tccagctttt
     6181 gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct ttttcctgcg
     6241 ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta tgtaaggttg
     6301 ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc tcactgtcac
     6361 gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc aaccccgatg
     6421 cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc tggctggcat
     6481 agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt tgattaagat
     6541 agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc gtcaccagaa
     6601 tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca agctgttcga
     6661 gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc cgttgcgcga
     6721 acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc agctggccat
     6781 tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc tcttcgacgt
     6841 ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt gcgggccagg
     6901 cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca atttcttcca
     6961 tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc acaggtccgg
     7021 gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat tacgctgctt
     7081 gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc cgggcagcgt
     7141 caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt tcaggaactg
     7201 ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa aagccgccta
     7261 gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc tacccgcctc
     7321 aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat taatattagt
     7381 gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc agcggcggtc
     7441 gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct tgagtcagaa
     7501 tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg actgaaaacg
     7561 cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga agccgccatt
     7621 gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg tgattactct
     7681 aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt tcaacggtca
     7741 ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct cattgtgatt
     7801 tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc gggccactgc
     7861 gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt gcctatattc
     7921 tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca atcatctgga
     7981 ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg gtcctgctgg
     8041 tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac gtgctgattc
     8101 agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg ggtgggattg
     8161 atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt gtcgcagaag
     8221 tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta tgagtgccgt
     8281 aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag ccagccgtga
     8341 ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc cgccggttga
     8401 cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg ttgccaaaag
     8461 ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa tcattaatcc
     8521 gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg caaaaagttg
     8581 tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt agcgactttg
     8641 gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa accggcctaa
     8701 cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc ggtttaccgg
     8761 caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg ggtatttact
     8821 ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa ctgttggaaa
     8881 cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg ctggtatatc
     8941 tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg ggcgtgatta
     9001 ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc gtaccttccg
     9061 aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc caccgtggtc
     9121 aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc gctgagtctc
     9181 tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa ctctcacgcg
     9241 ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga tctggacaat
     9301 gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg tggtacgccg
     9361 agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc gcgtttgccg
     9421 ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga agcggatcgc
     9481 tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca gagttttagc
     9541 gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa acgagcggca
     9601 aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca tggactaccg
     9661 gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact gaatccgccg
     9721 catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc gcgaccgccg
     9781 gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca gttattaacc
     9841 gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta tcagtgccag
     9901 cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg cgcgcacggc
     9961 aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca tcctcgtggt
    10021 tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga taagccgttt
    10081 gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga gtttattgcg
    10141 tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat tgcccagggt
    10201 tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt attcttaaac
    10261 tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc gcattaatca
    10321 cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact gaataaatat
    10381 cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg attataaata
    10441 tgtacaacct ggcgca
//
LOCUS       DAZSNO010000133        10446 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZSNO010000133 DAZSNO010000000
VERSION     DAZSNO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.133"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..794
                     /locus_tag="ACHWN2_003160"
     CDS             <1..794
                     /locus_tag="ACHWN2_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFR8932591.1"
                     /translation="SETKAAYRCFSNDKVSANKIMTPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="ACHWN2_003161"
     CDS             complement(744..1937)
                     /locus_tag="ACHWN2_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8932592.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="ACHWN2_003162"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="ACHWN2_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR8932593.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="ACHWN2_003163"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="ACHWN2_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR8932594.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="ACHWN2_003164"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="ACHWN2_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR8932595.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="ACHWN2_003165"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="ACHWN2_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR8932596.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="ACHWN2_003166"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="ACHWN2_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR8932597.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2560 a         2656 c         2749 g         2481 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgaccccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcata
//
LOCUS       DAZSNO010000134        10418 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZSNO010000134 DAZSNO010000000
VERSION     DAZSNO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10418
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.134"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            2..1369
                     /gene="dcuC"
                     /locus_tag="ACHWN2_003167"
     CDS             2..1369
                     /gene="dcuC"
                     /locus_tag="ACHWN2_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR8932598.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMPFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHWN2_003168"
     CDS             complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHWN2_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR8932599.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHWN2_003169"
     CDS             complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHWN2_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR8932600.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHWN2_003170"
     CDS             complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHWN2_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR8932601.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHWN2_003171"
     CDS             complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHWN2_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR8932602.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHWN2_003172"
     CDS             complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHWN2_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR8932603.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5324..5722
                     /gene="zapG"
                     /locus_tag="ACHWN2_003173"
     CDS             5324..5722
                     /gene="zapG"
                     /locus_tag="ACHWN2_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR8932604.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            5876..7223
                     /gene="degQ"
                     /locus_tag="ACHWN2_003174"
                     /pseudo
     CDS             5876..7223
                     /gene="degQ"
                     /locus_tag="ACHWN2_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            7313..8380
                     /gene="degS"
                     /locus_tag="ACHWN2_003175"
     CDS             7313..8380
                     /gene="degS"
                     /locus_tag="ACHWN2_003175"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR8932605.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHWN2_003176"
     CDS             complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHWN2_003176"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR8932606.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            9815..10285
                     /gene="argR"
                     /locus_tag="ACHWN2_003177"
     CDS             9815..10285
                     /gene="argR"
                     /locus_tag="ACHWN2_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR8932607.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2546 a         2654 c         2611 g         2607 t
ORIGIN      
        1 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
       61 aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt
      121 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      181 catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      241 tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt
      301 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      361 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      421 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      481 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      541 tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc ttccactacc agcttccggt
      601 cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga
      661 caaaaaagat aaaaatatca atcacgaaca ggcagagcaa aaaactctcg ataatgtccc
      721 gccgctctat tacgccattt tacctgtgat gccgttaatc ctgatgctcg gctcgctgtt
      781 cctcgcccac gtcgggctga tgcagtcaga actgcatctg gtggtggtga tgttactaag
      841 tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
      901 cgatgtgcag gcgttttttg acggcatggg tacgcagttt gtcaacgtgg taacgctggt
      961 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgttg atgcggttat
     1021 caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctagt
     1081 cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgc catttgccag
     1141 tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat
     1201 gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac
     1261 gtcaggaatt gcaggcgttt cgccttttgc ggtggtgaag cggacagcga tccccatggc
     1321 agtcggtttc gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa
     1381 gacaaaacag gccgcctggg cctgttttgt attacttcac aacgcgtaat gccggtcgac
     1441 caccgcgtgg tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct
     1501 tgtcgccatc aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca
     1561 tgatgctggt atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt
     1621 cacgggcgta gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccac
     1681 caaagcgcgc gttaaagcgc acctcatcat tcgccagttc cagattgccg acagcacgcg
     1741 gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag
     1801 ggagcgtcac atccaccacc aggtgcggcg tgagctggtt atccagcaac cactcataga
     1861 atgcacgcag cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact
     1921 ccggcccaga cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc
     1981 aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc gggccgctga actcgatgcc
     2041 cagttgcggc agacgccaca gcagcggagc aaggtagcaa tcgaccaggc tgaactcatc
     2101 gctcaggaag tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag
     2161 ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg atgatagtgt tcatcagcgt
     2221 gtaccagtct ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta
     2281 aacaggcatc agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga
     2341 ttcccacagg gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc
     2401 aatcagatcc tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt
     2461 ctcagccagc acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt
     2521 cattaccgaa cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt
     2581 ttactgctac cagccaccag gtggccagtc agaagttgtg ttacccaata aggaacgact
     2641 ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg
     2701 atgatagttt accagatttt gcgacctttg tggtgagtgg atgccggaaa tggggaaaaa
     2761 gagatgcgct ttagtctgaa atagttgact tagaccctta ttggcgatgt ggtttttgtt
     2821 ttacctgtct ttcagacggc agcaaaaagc aacttcccat tttttacgct gattcagaat
     2881 ttagccataa aaaaacccgc cgaagcgggt tttttcgcaa attgttttct gccgtagcag
     2941 aagccaatta acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct
     3001 tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca
     3061 gggactcgtc gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac
     3121 cagagatacc accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca
     3181 gttccagcgg ctgacgaact accatgcggg cagtttcacg accgaagtac tgttccagag
     3241 aacgttggtt gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac
     3301 ttttgcggcg accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga
     3361 tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca
     3421 gtttacggaa catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt
     3481 caatcacacg ctcaggacgg cgagcaatca tctcttcaaa ggtcgcttgt ttgataccac
     3541 cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa
     3601 ctttgtcagc gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtatattccg
     3661 ctttgtgctt accgcgcagg cgacgagcca gttcagtagc cagacggccc agagttttac
     3721 cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt
     3781 tcattaaaag cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt
     3841 gttgaggttc acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac
     3901 acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt
     3961 cggggtcaaa gatcgacccc tttttgtgat ttgtggcagg ttttaacccg ccaaatgctc
     4021 gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc
     4081 aaacttcagc cgctcaccct gataaatttc atacagcggc acttctgcac tcaccactaa
     4141 tttgacatgg cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc
     4201 catcaaccgc gtcatgactg gcacatcaaa caacattact gtgtgaaaca ggcgtgagag
     4261 cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag aaaccgccag
     4321 cgtctggttc tcgacgccca ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga
     4381 attttctcgt ttcacccccg ccagcgccaa ccatagttta tccatctgcg cccgggtttc
     4441 atcgttgagt ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac
     4501 accagcgtcc acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa
     4561 acgcgcacgt tgcaggccat ttcgataaag ttcgtccggc ggaatatttg acgtcgctac
     4621 cagggtaata ccgcgggtga acagggcttt catcagaccg ccaagtagca tggcgtcggt
     4681 aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc
     4741 ggcaataatt tccagcggat cggtctgccc ctgtaaggca gttagctctt cgtgcacccg
     4801 cagcataaaa cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa
     4861 aaggtccatc agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac
     4921 tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc
     4981 actcgtcctg ggggctggtg gcctgctatt gatgagttct tgataaataa tttccaggcg
     5041 gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag
     5101 gtattgcgat gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt
     5161 gcgccgttgt tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg
     5221 gattccactt ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct
     5281 gaagccaaca ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc
     5341 gctaattggg ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg
     5401 taaactacgc cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga
     5461 cgagtatcgc gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat
     5521 ggcgcacgac tatcgccagc tgtatcagca catggcaaaa agctccagca gcctgctgcc
     5581 ggaactgtct gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa
     5641 cgatcaggca ccggtgcagg tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg
     5701 tactggcgcg aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct
     5761 ctccctcccc gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata
     5821 acatcaaact gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa
     5881 aaaacaaacc cagctgttga gtgcattagc gttaagtgcc gggttaactc tctcggcgtc
     5941 atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct
     6001 ggctccaatg ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc
     6061 cagtcaggga cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga
     6121 tcaacctgca caacccttcg aaggtttagg ttccggtgtc atcatcaacg ccagtaaagg
     6181 ctatgtgctg accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa
     6241 tgatgggcgc gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct
     6301 gttataaatt caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt
     6361 gcgcgtcggt gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac
     6421 ctctggcatt gtttccgcat taggccgcag cgggttgaat cttgaaggtc tggaaaactt
     6481 tatccagaca gatgcttcca ttaaccgcgg taactccggc ggtgcactat taaaccttaa
     6541 cggtgagtta attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat
     6601 tggatttgcc atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg
     6661 tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa
     6721 agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caagttctgg
     6781 ctcagcgaaa gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct
     6841 gaatagcttt gctgagttgc gctctcgtat cgcgactaca gagccgggca cgaaagtgaa
     6901 gcttggcctg ctgcgtaacg gcaaaccact ggaagtagaa gtgacgcttg ataccagcac
     6961 ctcttcgtcg gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg
     7021 gcaaaggtat taagatagat gaagttgtca aaggaagccc agctgctcag gctggcttgc
     7081 aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc
     7141 gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca aattgtacgc ggcaatgaaa
     7201 gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggctgacg tgtgatgtcc
     7261 ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca tcatgtttgt
     7321 gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc tggttgccat
     7381 gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta ccgatgagac
     7441 gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta acgtttacaa
     7501 ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg gatccggtgt
     7561 aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg acgccgatca
     7621 gatcatcgtc gccttacagg atggacgtgt atttgaagca ttgctggtgg gatctgactc
     7681 tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta ccattccaat
     7741 taatgcacgt cgcgtaccgc acattggcga cgtagtactg gcgatcggta acccgtacaa
     7801 cctcgggcag accattaccc aagggattat tagtgccacg ggtcgaatcg gtctgaaccc
     7861 gaccgggcgg caaaacttcc tacaaaccga tgcttccatt aaccacggta actctggcgg
     7921 cgcgctggtg aactcgctgg gagaactgat gggcattaac acgctgtcgt ttgataagag
     7981 taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa
     8041 aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg gtatcggcgg
     8101 acgcgagatc gcaccactgc acgcgcagag cggtggtata gatcaactgc aagggatcgt
     8161 ggttaatgaa gtgtcacctg acggcccagc ggcgaatgcg ggtattcagg tcaacgatct
     8221 gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg atcaggtggc
     8281 ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata agcagttaac
     8341 gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct caaaacaaaa
     8401 accggagtct gtgctccggt tttttattat tcgctaatca attacttatt aacgaactct
     8461 ttgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc gttctgttca
     8521 aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc cagcagcagc
     8581 ggttgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca ttcgacaacg
     8641 ccttgttcgc cctgcagtgc acgaaccaga gacagaccaa aacgtgcagc tgcctggccc
     8701 atagacaggg ttgcagaccc gccaccggct ttcgcttcaa ccacctcagt acccgcgttc
     8761 tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc aggaacctgt
     8821 gacagcagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac ttcaacttcg
     8881 cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat atccagcgtg
     8941 gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac ttcagcagca
     9001 attgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt cgggcagttt
     9061 ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacgttaaa caggtcggaa
     9121 cgatccatac ccggtttacg cgctacgcct gcagagataa gaacgacatc tgcgccttcc
     9181 agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt agggatatgg
     9241 ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga gagttctgaa
     9301 cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc gccagcagcg
     9361 ccgaggactg cgactttcat cctaaactcc ttattatatt gataaactaa gatatgttgc
     9421 tccgctgccg cgaccttaat ccacaaaatt gccgtttaca atgaccacat ctcaagaatg
     9481 tgtagtcacg caagtttagc gtttatgcat ttaattgccg taatcaggaa cctaacttac
     9541 gtaattaaca gtcgcttatg cattagcgca acattccaac aggtggtgac aatataccct
     9601 accgttcagc caaaacaaca tcaatttgat aacaattaat ttacttttaa acagaatttg
     9661 catgccgtga cgcaggcatg tttctcaata acgaaatttg ataaaatccc gctctttcat
     9721 aacattattt cagccttctt cagggctgac tgtttgcata aaaattcatc tgtatgcaca
     9781 ataatgttgt atcaaccacc atatcgggtg acttatgcga agctcggcta agcaagaaga
     9841 actagttaaa gcatttaaag cattacttaa agaagagaaa tttagctccc agggcgaaat
     9901 cgtcgccgcg ttgcaggagc aaggctttga caatattaat cagtctaaag tctcgcggat
     9961 gttgaccaag tttggtgctg tacgtacacg caatgccaaa atggaaatgg tttactgcct
    10021 gccagctgaa ctgggtgtac caaccacctc cagtccattg aagaatctgg tgctggatat
    10081 cgactacaac gatgcagttg tcgtgattca taccagccct ggcgcggcgc agttaattgc
    10141 tcgcctgctg gactcactgg gcaaagcaga aggtattctg ggcaccatcg ctggcgatga
    10201 caccatcttt accacccctg ctaacggttt caccgtcaaa gacctgtacg aagcgatttt
    10261 agagctgttc gaccaggagc tttaatctct gccccgtcgc ttctggcggc ggggaaaatg
    10321 ttgcttatcc ctctcattcc ccttctttct cctgcgatac atttaacgaa tagtgcgttt
    10381 taccgcgaca catcattcac aaaatgaata catacggt
//
LOCUS       DAZSNO010000135        10182 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZSNO010000135 DAZSNO010000000
VERSION     DAZSNO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.135"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1294
                     /gene="traN"
                     /locus_tag="ACHWN2_003178"
     CDS             <1..1294
                     /gene="traN"
                     /locus_tag="ACHWN2_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFR8932608.1"
                     /translation="MNGLKLVFSVTAPVTGTVESASLSVYAAFFFLNSRYTFMNTTFN
                     VGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDRKVYESLVSGAST
                     FTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKTGVMEGKPWNITQ
                     ACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLHEYATYSCESRTS
                     GKVMVCGGDVFCLDGECDKAQSGKNSDFGEAVSQLAALAAAGKDVAALNGVDVRAFTG
                     EAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLTVSVGEFCSRKVL
                     GVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGITVDELQKIQFDRL
                     DFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            1321..1578
                     /locus_tag="ACHWN2_003179"
     CDS             1321..1578
                     /locus_tag="ACHWN2_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFR8932609.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            1571..2314
                     /gene="traF"
                     /locus_tag="ACHWN2_003180"
     CDS             1571..2314
                     /gene="traF"
                     /locus_tag="ACHWN2_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFR8932610.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            2328..2669
                     /gene="trbA"
                     /locus_tag="ACHWN2_003181"
     CDS             2328..2669
                     /gene="trbA"
                     /locus_tag="ACHWN2_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFR8932611.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(2650..2985)
                     /locus_tag="ACHWN2_003182"
     CDS             complement(2650..2985)
                     /locus_tag="ACHWN2_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFR8932612.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            3066..3350
                     /gene="traQ"
                     /locus_tag="ACHWN2_003183"
     CDS             3066..3350
                     /gene="traQ"
                     /locus_tag="ACHWN2_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFR8932613.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            3337..3882
                     /gene="trbB"
                     /locus_tag="ACHWN2_003184"
     CDS             3337..3882
                     /gene="trbB"
                     /locus_tag="ACHWN2_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFR8932614.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            3812..4159
                     /gene="trbJ"
                     /locus_tag="ACHWN2_003185"
     CDS             3812..4159
                     /gene="trbJ"
                     /locus_tag="ACHWN2_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFR8932615.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            4140..4532
                     /gene="trbF"
                     /locus_tag="ACHWN2_003186"
     CDS             4140..4532
                     /gene="trbF"
                     /locus_tag="ACHWN2_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFR8932616.1"
                     /translation="MKEKINDTEPGIKQIEREIERGCDNAKKYFWLFVVFFAAGLIVR
                     NVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLAAASLSPLCEII
                     FGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            4519..5892
                     /gene="traH"
                     /locus_tag="ACHWN2_003187"
     CDS             4519..5892
                     /gene="traH"
                     /locus_tag="ACHWN2_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFR8932617.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            5889..8714
                     /gene="traG"
                     /locus_tag="ACHWN2_003188"
     CDS             5889..8714
                     /gene="traG"
                     /locus_tag="ACHWN2_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFR8932618.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            8711..9220
                     /gene="traS"
                     /locus_tag="ACHWN2_003189"
     CDS             8711..9220
                     /gene="traS"
                     /locus_tag="ACHWN2_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFR8932619.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            9234..9965
                     /gene="traT"
                     /locus_tag="ACHWN2_003190"
     CDS             9234..9965
                     /gene="traT"
                     /locus_tag="ACHWN2_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFR8932620.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
BASE COUNT         2625 a         2248 c         2723 g         2586 t
ORIGIN      
        1 tatgaacggg ctgaaactgg tgttcagtgt cacggctccg gtaaccggta cagtggaaag
       61 tgcatcattg tctgtatacg ccgctttttt cttcctgaac tcccgttata ccttcatgaa
      121 tacaaccttt aatgtcggcc tggcaaacgg gcagtcggat acttaccccc tgtccggggc
      181 gacaggtttg caggtgacac aggggcaggt tctgaccggt agtggctgta ctgctaacgg
      241 taattgcctc ccgcacggga atggtgacag gaaagtctat gaatcgcttg tcagtggtgc
      301 ttccaccttt acgctgaaac tcaggatgaa ggtccgtgat aaagaatggg ttccccgggt
      361 ggagtgggtg gagagttgtc cgtttaataa agcggatggc gtgctgacag gcactgagtg
      421 ttcagagccg ggtggtacga aaaccggagt gatggaaggt aaaccctgga acatcacaca
      481 ggcatgctgg gcataccggg ataaatatgt cactcagtcc gcagataacg gcacctgtca
      541 gaaatatgtc gataatccgg cctgtacgct ggcctcacgt cagtgcgcgt tttattccga
      601 tgagggaacc tgtctgcatg agtacgccac gtactcctgc gagtccagaa catcaggaaa
      661 agtgatggtc tgtggtggtg atgtcttctg ccttgacggt gaatgcgata aggcacagag
      721 cggaaaaaac agtgattttg gtgaagcagt atcacagctg gctgcgcttg ccgccgcagg
      781 taaggatgtt gccgccctga atggtgtgga tgtcagggct ttcacgggtg aggcgaaatt
      841 ctgccgtaag gccgcagccg gcttcagtaa ctgctgtaag gatggcggct ggggtcagga
      901 tgtggggctg gcgaagtgca acagtgaaga aaaggcgctg ggaaaggcaa aggacaacaa
      961 actgacggtg agtgtgggcg agttctgctc ccggaaagtg ctgggtgtct gtctgcagaa
     1021 aaagcgcagc tactgccagt ttgattcgaa gctggcgcag atagtccagc agcaggggcg
     1081 taacgggcag ttgcgtatcg ggtttggcag tgcgaagcat cctgactgtc gggggattac
     1141 ggttgatgag ctgcagaaaa ttcagttcga cagactggat ttcactaact tctacgaaga
     1201 cctgatgaat aaccagaaaa tcccggacag tggcgttctg acgcagaaag tgaaagagca
     1261 gattgctgac cagctgaaac aggcaggaca gtaaggtatg gaaaataacc gggggaaatg
     1321 atgaaagtta tattcacttc taatcgtctt gttgattttc ttatccggct ttcgattaca
     1381 gcgattgtca tttctccggt aattatctgg tcgtgggata cggtaaaaga aactacggca
     1441 gacgggatac ttgctgctgc tttcgttatc ctttactccg gtgtgctcct gtttattctg
     1501 tatttttgtt tttctgccct gactggcctg cagaaagatg atgaaagaaa aaatgatgaa
     1561 aggaatgaag atgaataaag cattactgcc actgttactc tgctgcttta tttttccggc
     1621 gtcagggaaa gatgcaggct ggcagtggta taacgagaaa ataaatccga aggaaaaaga
     1681 aaataaacct gtacctgcag ccccccgtca ggagccggat attatgcaga aactggccgc
     1741 gctgcagacg gcaacaaagc gggcgctgta cgaagccatt ctgtatcccg gcgtggataa
     1801 ttttgttaaa tatttccggc tgcagaatta ctggactcag caggccgggc ttttcaccat
     1861 gagtgctaaa aaggccatgc tggcacatcc cgaactggac tataacctgc agtacagcca
     1921 ttacaacggc acggtacgga accagctggc ggcagaccag gcgcagcagc gacaggccat
     1981 tgcgaaactg gctgaacact acggcatcat gtttttttac cgggggcagg accccatcga
     2041 cgggcaactg gcgcaggtca ttaatggctt ccgggatacg tatggtctga gtgttattcc
     2101 cgtttccgtg gatggcgtga ttaatccgct gttgccggat tcccggactg accaggggca
     2161 ggcgcagcgc ctcggtgtga aatatttccc ggccatgatg ctggttgacc cgaaacaggg
     2221 cagtgttcgc ccgttatcat acggctttat ttcacaggac gacctggcaa aacagttcct
     2281 gaacgtttct gaagatttta aaccgaattt ttaaccaaag gttatatatg catacagggg
     2341 atttgtgttt ttttacaggt gccagcctgt ttatcgtggg ggttgttggt tatttcttta
     2401 caggaccaaa taataaagga gtttatctgc tgagctcgtt tgtcttcttt gtagtaaatg
     2461 tcataagtgt atgtatgctg ccagataatt tcttgtttct ttgtggtgcg gttatgaatc
     2521 agtgtgccgt accggttgtt tcaatggcag tcagtaccat accggttatc acgaagtttg
     2581 acagtttcag aaggatgttt gtttgtatta tgttgtcagc catatggtct ggagtgatgt
     2641 ggttttttat tatttcatta gtgaaatgac tggtgctaat ttgtcataaa tgccaaatcc
     2701 aatagtgagg ttgattacca cagaccagag gataaaccag actgctatcc agtgacttct
     2761 gaataactca caaaaataaa tatagatgat tagaaatagc catatcacaa cagcgataat
     2821 cgctataaat aacagtatcg tcacaaataa atcagcaaag gttttaatgg ggagggagtg
     2881 caataatgaa tacaccagta aagcaaaagc cgcattacct gataatgact ctcttatgat
     2941 gtttctcact atttccagtt tttctttaaa agataacttt ttcatttgta cctctgatta
     3001 atatcacaca aacgcgaata ttacagacat gaatactctc atgtaaataa taaccggagt
     3061 ttaacatgat aagtaaacgc agattctctt taccccggct tgatattacg ggaatgtggg
     3121 tattttccct gggtgtctgg tttcatatcg tcgcccgtct tgtttacagc aaaccctgga
     3181 tggctttttt tctggcggag ttgattgcgg ccatccttgt gctgttcggc gcataccagg
     3241 tgctggatgc atggattgca cgtgtcagcc tggaagagcg tgaggcactg gaagccaggc
     3301 aacaggccat gatggaaggg cagcaggagg gcggacatgt ctctcactaa attactgccg
     3361 gtcattttgt tgatgatggc cacaggtgtg caggcctcca cccgggatga aattgaacga
     3421 ctctggaatc cgcagggaat ggccgtgcag cctgcacaac cggcagcaga cacatcagcc
     3481 agaacagaaa agccggctcc ccgctggttc cgcctcagca acggcaggca ggttaacctg
     3541 gccgactgga aggtggtgct gtttatgcag gggcattgcc cttactgtca ccagtttgac
     3601 ccggtactga agcagctggc acagcagtac ggcttttccg tttttcccta caccctggat
     3661 ggtcaggggg atactgcctt tccggaagca ttaccggtgc caccggacgt gatgcagacc
     3721 ttcttcccga atatcccggt ggccacaccg accaccttcc tggtcaacgt caacacactt
     3781 gaggcattac cgcttttgca gggggcaacg gatgccgcca gttttatggc gcggatggat
     3841 accgttctgc agatatacgg agaggaaaaa ggtgcgaaat aagcaggtgg tgttactcat
     3901 tgccggaata tccggcatcg cgacgggaat aatcgtcagc ctgaatatcc cctttatccg
     3961 tcaggggctt ttttatcccg ccagccctgt ggaaattgtc gtttcgctgt gtcttacctt
     4021 ttctgtttct gttgtgtttt ttgtgggggc aattgtggga tggatttcgg tgtctgaaat
     4081 atattacagc cgcatgacct gtctgcatga atcatcagaa atatcagagg gaacttatga
     4141 tgaaagaaaa aataaatgat acagaaccgg gaatcaaaca aattgaaagg gagattgaac
     4201 gtggttgtga taatgcaaaa aaatacttct ggctttttgt ggttttcttt gctgccggat
     4261 tgattgtaag gaacgttatg catgattttt tcagtgccgg tattgattcg tggaaagctg
     4321 acccggaatt aaataatttt cgttatatgt ggaacaccct gatgtatgtc attccgatta
     4381 tgttatatgc gcttgcagct ggttttcttg ctgcggcttc tctgtcgccg ttgtgcgaaa
     4441 taatatttgg gggtgtccgg atattcctgc tgaaacgccg tatgcgccgg gagaacacat
     4501 taagggaggg cagcaataat gcttcgcatt aaacctcttc tggttctttg tgccgcttta
     4561 ctgacggtca cacccgcagc gtcagcggat gtgaacagcg atatgaatca gttctttaac
     4621 aagctgggct tcgcctccaa taccacacag ccgggcgtct ggcaggggca ggccgccggg
     4681 tatgcctatg gtggctccct gtatgcccgc acccaggtca aaaatatcca gctgatttcc
     4741 atgacgctgc cggatatcaa tgccggatgt ggcggcatcg acgcctatct cggctcgttc
     4801 agttttatta atggcgatca gttgcagcgt tttgttaagc agattatgag caatgctgcc
     4861 ggttactttt ttgaccttgc cctgcagaca acggtgccgg aaataaaaac cgcaaaagac
     4921 ttcctgcaga aaatggcaag tgacattaac agtatgaacc tcagttcctg tcaggtggca
     4981 caggggcttg tgggggggct gttccctcag acgcaggaat ctcagcagaa agtttgtcag
     5041 gacattggca gtgaaagcaa tatcttcgcc gactgggccg catctcgtca ggggtgcacg
     5101 gtcggagggc agtcctcatc tgttcgtgac aaagccagcg acaaggacaa ggagcgggtg
     5161 ctgaaaaacc agaatatcat atgggaggcg ctgaaaaaaa acagaatgtt tgacggcaac
     5221 aaagagttga aagaatttat catgacgctc accggtacgc tgatttttgg ggaagacagc
     5281 gagattaccc ccctgccggc gcgtaccact gaccgcgata tcctccgggc gattatggag
     5341 gggggaacgg caaaaattta ccactgcaac gattcggaca agtgtctgaa ggtggtggcg
     5401 gatgcaaatg tgaccatcgc ccgggataaa gccctgaagt cacagattac aaaactgctc
     5461 accagtattc agaataaagc agtgagtgac actccgcttg acgcccggga acaggggttt
     5521 atttccagca ccaccattcc cgtttttaaa tatctgattg acccgcagat gctcggtgtg
     5581 tccaacagcg tcgtttacca gctgacggac tatatcggct acgacatcat gctccagtac
     5641 attcaggaac tgcttcagca ggcccgggcc atgattgcca ccgggaacta tccgcaggct
     5701 gtgatggaca atgttctgga gaatctgaat caggcacagc aacagattgc cattttccag
     5761 agtcaggtac aggtccagca ggatgcgctg ctggttgtcg aacgtcagat gaactacatg
     5821 cgtcagcagc tttccgcccg tatgctcagc cgttaccaga acaactatca cttcggaggg
     5881 aattcactgt gaatgaagtt tatgtgattg ccggtggtga gtggttgcgg aataacctga
     5941 acgccattgc tgcatttatg agtacccgga cctgggattc aattgaaaaa atcgcgctca
     6001 ccctgtctgt tgttgctgtg gcttttatgt gggtacagcg gcataacgtg atggatttgc
     6061 tgggctgggt ggccgtgttt gtgcttatca gcctgctggt taatgtccgc acatcggtgc
     6121 agattattga taacagtgac ctggtcaaag tccatcgggt ggataatgtt ccggtcggtc
     6181 tggcgatgcc actttctctt acgacccgta tcgggcatgc gatggtggtc agctacgaga
     6241 tgatattcac ccaaccggac agcgtcacct acagcaagac aggcatgctg ttcggggcga
     6301 atctgattgt gaaaagcacc gatttcctgt cccggaatcc ggaaatcatc aatctgttcc
     6361 aggactacgt ccagaactgc gtgctgggtg atatttacct gaatcataaa tacacgctgg
     6421 aagatctgat ggcctccgct gacccctaca cgctgatttt ttcccgaccc agcccgctac
     6481 ggggcgttta tgacaacaat aataatttca taacctgtaa ggatgcgtcg gtcacgctga
     6541 aagacaggct gaatctcgat acaaagacgg gaggcaagac ctggcattat tatgtgcagc
     6601 agatatttgg cggcagaccg gacccggacc tgttattcag acaactggtc agtgacagtt
     6661 acagttattt ctacggctcc agtcagtctg ccagccagat tatgcgccag aacgtcacaa
     6721 taaatgccct gaaagagggt atcaccagta atgctgcccg taatggtgac accgccagcc
     6781 tggtgaatct ggccaccacg tcatcgatgg agaagcaacg tctggcgcat gtctctatcg
     6841 gtcatgtgac aatgcgaaac ctgccaatgg tacagactat cctgacggga attgccattg
     6901 gtattttccc gcttctggta ctggcagctg tcttcaacaa actgacactg tccgtcctga
     6961 aaggctatgt gtttgccctg atgtggctgc agacctggcc gttgttgtat gccattctga
     7021 acagtgccat gacattctat gcgaagatga atggtgcgcc ggtcgtgctg tctgaactct
     7081 ctcagataca gctgaaatac tctgacctgg cctctactgc cgggtatctt tcagccatga
     7141 tccccccgtt gtcatggatg atggtaaaag gccttggcgc aggtttttcc agcgtgtaca
     7201 gccacttcgc ctcttcctct atcagtccga cggccagtgc agcaggcagt gtggttgacg
     7261 gtaattactc ctacggcaac atgcagacgg aaaacgtgaa cggcttcagc tggagcacca
     7321 acagcaccac gtcgtttggt cagatgatgt accagaccgg cagtggcgca actgccacac
     7381 agacccgtga cggtaatatg gtgatggatg caagcggagc gatgtcccgt ttaccggtcg
     7441 gtatcaatgc aacgcgtcag attgcagcgg cgcaacagga aatggcccgg gaggcgtcga
     7501 acagagcaga aagtgccctg catggtttca gcagcagtat tgccagtgcc tggaacacgc
     7561 tcagtcagtt tggttctaac cgggggagca gtgattctgt caccggtggc gctgacagca
     7621 ccatgagtgc acaggactcc atgatggcca gccgtatgcg cagtgcagtg gaaagctatg
     7681 cgaaggcgca taatatcagt aatgagcagg cgactcagga actggcatca acaggttcta
     7741 agttctcgta tggcggatat gcagatgctc atgctgaatg ggggattaaa cctggaattc
     7801 ttggtgttgg tggtggggtt ggtgtcagag gcgggtttaa gggaagtctt gatcgaacgg
     7861 atgatgattc acattctgca agtagtggca gcagagctac ccatgatgct cgccatgata
     7921 ttgacgctaa agccagtaag gactttaaag aagccagcga ttactttacc agtcgcaagg
     7981 tcagtgagtc cggcagccat actgacaata atgctgactc ccgggtggac cagttgtctg
     8041 cagccctgaa ctcagcgaaa cagagttacg accagtacac gacgaatatg acccgcagcc
     8101 atgaatatgc ggaaatggcc tcccgtactg aaagcatgag cgggcagatg agtgaagacc
     8161 tgtcgcaaca gtttgcacag tatgtgatga aacgcgcacc gcaggatgca gaagctattc
     8221 tgacaaatac cagttcgccg gagattgcag aacgtcgtcg ggcgatggcc tggttttttg
     8281 tgcaggaaca ggtacagcct ggtgttgata atgcctggcg ggaatcccgt ggcgatattg
     8341 gtaaaggaat ggagagcgta ccttcgggtg gtggcagtca ggatattatt gctgaccatc
     8401 agggacatca ggccattatt gagcaaagaa cgcaggacag taatatccgt aatgatgtaa
     8461 aacatcaggt tgataatatg gtcacagaat atcgtggcaa tatcggagat actcagaaca
     8521 gtatccgtgg cgaggaaaat attgtaagag ggcagtattc tgaactgcaa aatcatcata
     8581 aaacagaggc attatctcag aataataaat ataatgaaga gcgagaaagt caaaagataa
     8641 tacctgagcc atcagaagag gctgtgaaaa aaatggttga tgataaaaag gatagactga
     8701 aggggccatt atgatcactc actatgatat aaaaacggag acacagaaac taaaggatgt
     8761 actctctgta gagggagtta atattccgcc attattacaa gtcataaaac caggggtgta
     8821 tgtgttttta tgggttttat tatggccgac atttcttcgt ttgctggcag ataaagttga
     8881 tatccgagat gctggatttg atatatgttt tagtggcgta atgggtttta tcctttttgt
     8941 tgccattact aatggaatga tgttgtatct tgccatccct aaaaaattca gagatgagtc
     9001 gaaagttata tcttttatgt atgataagaa caaaacttat atcttgtcgt ttgtgattgt
     9061 tttttcaata gtttcctttg ctcatacgtt cttatttggg tttttatcaa taactctgtt
     9121 tgtaattatt tcctttattt atactataga tataaatcgt tataatctct ctgcaatagt
     9181 ctcagtaata ggtctgttca aaaaagagtc tgttagttaa aaggatattc tggatgaaaa
     9241 caaaaaaaat gatgatggtt gcactggtca gttccactct ggccctttca gggtgtggtg
     9301 cgatgagcac agcaatcaag aagcgtaacc ttgaggtgaa gactcagatg agtgagacca
     9361 tctggcttaa acccgccagc gaacgcacgg tatttctgca gatcaaaaac acgtctgata
     9421 aagacatgag tgggctgcag ggcaaaattg ctgatgctgt gaaagcaaaa ggatatcagg
     9481 tggtgacttc tccggatgca gcctactact ggattcaggc gaatgtgctg aaggccgata
     9541 agatggatct gcgggagtct cagggatggc tgaaccgtgg ttatgaaggc gcagctgttg
     9601 gtgcagcgtt aggtgccggt attaccggct ataactcaaa ttctgccggt gccacactcg
     9661 gtgtgggcct tgctgctggt ctggtgggta tggctgcaga tgcgatggtg gaagatgtga
     9721 actataccat gatcacggat gtgcagattg cagagcgtac taaggcaacg gtgacaacgg
     9781 ataatgttgc cgccctgcgt cagggcacat ccggtgcgaa aattcagacc agtactgaaa
     9841 caggtaacca gcataaatac cagacccgtg tggtctcaaa tgcgaacaag gttaacctga
     9901 aatttgaaga ggcgaagcct gttctcgaag accagctggc caaatcaatc gcaaatattc
     9961 tctgaacctc gtcaggaggc tgatctgacc ggcctccttg ctgacgcctc gcgttgctcg
    10021 ttgtccaacc ccgggatgta acaaaataaa ttttgtcacc tcccggcgtt tccgaaataa
    10081 atatgaaaac actgagcggt tgtgcaaatt ctttgtgcga ccgtgaagtg ttgccggcga
    10141 agccgggatc ttccggagaa agtcattatg acatcagaca at
//
LOCUS       DAZSNO010000136        10059 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZSNO010000136 DAZSNO010000000
VERSION     DAZSNO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10059
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.136"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..620
                     /locus_tag="ACHWN2_003191"
     CDS             <1..620
                     /locus_tag="ACHWN2_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HFR8932621.1"
                     /translation="VQRLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATRPN
                     EKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPH
                     EHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLD
                     EFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            717..869
                     /gene="hokE"
                     /locus_tag="ACHWN2_003192"
     CDS             717..869
                     /gene="hokE"
                     /locus_tag="ACHWN2_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR8932622.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            1220..1372
                     /gene="hokE"
                     /locus_tag="ACHWN2_003193"
     CDS             1220..1372
                     /gene="hokE"
                     /locus_tag="ACHWN2_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR8932623.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1494..2114)
                     /gene="entD"
                     /locus_tag="ACHWN2_003194"
     CDS             complement(1494..2114)
                     /gene="entD"
                     /locus_tag="ACHWN2_003194"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR8932624.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(2289..4529)
                     /gene="fepA"
                     /locus_tag="ACHWN2_003195"
     CDS             complement(2289..4529)
                     /gene="fepA"
                     /locus_tag="ACHWN2_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR8932625.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4772..5974
                     /gene="fes"
                     /locus_tag="ACHWN2_003196"
     CDS             4772..5974
                     /gene="fes"
                     /locus_tag="ACHWN2_003196"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR8932626.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5977..6195
                     /gene="ybdZ"
                     /locus_tag="ACHWN2_003197"
     CDS             5977..6195
                     /gene="ybdZ"
                     /locus_tag="ACHWN2_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR8932627.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            6192..10037
                     /gene="entF"
                     /locus_tag="ACHWN2_003198"
     CDS             6192..10037
                     /gene="entF"
                     /locus_tag="ACHWN2_003198"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR8932628.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2249 a         2603 c         2744 g         2463 t
ORIGIN      
        1 ctgttcagcg cctgatggga accctctcac ttaaagcagc aattaaggtc aagcgatacc
       61 gctcttacag aggagaggta gggcaaaccg cccctaatgt tctccaaaga gatttcaagg
      121 ctacgcggcc aaacgagaag tgggttaccg atgttactga atttgcagtc aatgggcgca
      181 agctgtattt gtctccagta atagatctct tcaacaacga agttatttct tacagccttt
      241 cggaaagacc agtgatgaac atggttgaga atatgctcga tcaggcattc aaaaagctta
      301 atcctcacga gcatcctgtt ctgcactctg accagggatg gcagtatcgt atgagaagat
      361 atcaaaatat ccttaaagaa catggtatta aacaaagcat gtccagaaaa ggcaattgtc
      421 tggataatgc tgtggtggag tgtttctttg gaaccttaaa gtcggagtgt ttttatcttg
      481 atgagttcag taatataagc gaactgaagg atgctgttac ggaatatatt gaatactata
      541 acagcagaag aattagcctg aaattaaaag gtctgactcc aattgaatat cggaatcaga
      601 cctatatgcc tcgtgtataa ctgtccaact ttttggggtc agtacaccct ggggctgcca
      661 ctccaaacca gacaatttgg atggtagtcc cttcttcgca tggaggtaat ataaacatgc
      721 tgacgaaata tgcccttgtg gcagtcatag tgctgtgttt gacggtactg ggatttacgc
      781 ttctggccgg agactcgctg tgtgagttta cggtgaagga acgtaatatt gagttcaggg
      841 ctgttctcgc ttacgaaccg aagaagtagc cgttgtgcgg ggagtaatcc ccgcatatcc
      901 ggttgtcagg tcaggatggt aaggcacctg ctttacactt tcgcccgcgg tcagtgatgg
      961 ctgcgggcga tttcgtttta agagcaatga tgagttttgc cgacagaagt ccgaaacagg
     1021 actcactgta taaataaaca gctattttgc taaggaaggg taagataacg gcgggtgcct
     1081 gaagctttcc ggtttcaggt taactctgag gtttggaaag atgaagcccc aagagatatt
     1141 tctatcaatc ctggggctgc cactccgaac ctcaacaact cggatggtag tcccttcttc
     1201 gcatggaggc aatataaaca tgctgacgaa atatgccctt gtggcagtca tagtgctgtg
     1261 tttgacggtg ctgggattta cgcttctggt cggagactcg ctgtgtgagt tcaccgtaaa
     1321 ggaacgtaat attgagttta aggctgttct tgcttacgaa ccgaagaagt agccgttgtg
     1381 cggggagtaa tccccgcata tccggttgtc aggtcagagt ggtaaggcac ctgctttaca
     1441 ctttcgcccg cagtcagtga tggctgcggg cgaatcgtac cagatgttgt caattaatcg
     1501 tgttggcaca gagttatgac tattttttct tttatctgcc agtgcacagc aaacatctca
     1561 ttctcacgat gaatgatgac ctgctgttta ttccagctaa ttatctgata gtccagaaaa
     1621 cctgcatcag tttggatctc acttgcctta aatgcgctct ctttggcgga aaatgcaagt
     1681 gtcagcgcca gagaaaaggc taaaccgcag tctgcgagtc gctcgtgttc tgctggtgta
     1741 ataatgttgt ctgtcaattc ggtcgctgtt tgcgcagaaa aaatttcttc gatatcaacg
     1801 ccaatcggtt gacgggaaac cacggccaat gccgtagccc cgcagtggct aatactgccg
     1861 tatacctccg caggccacac cggttggcgt agctcgccga ttgcgggcac acatttatag
     1921 ccatattccc gcaaggcata aacagcagcg atccgtccgg ctaaatgctc tgttttacgt
     1981 ttacgtccag cgtgttgcag ttgtgcgtag tgcggcagcc agagtaaatc ctgctcacaa
     2041 aaattcgccg gatcgaactc aacaaaatgc agcgtatgtc cggcaaaggg gagggaggta
     2101 tgcgtagttt tcatatcgac catattcgag actgatgaca aacgcaaaac tgcctgatgc
     2161 gctacgctta tcaggcctac gtgatcctgc aatatattga attggcaaga tctttgtagg
     2221 ccggataagg cgttcacgcc gcatccggcg tgaacgacgc gcactttgtc aacaatctga
     2281 cgttagcatc agaagtgagt attaatgctc atataccacg tacgtcccgg ctcgttatag
     2341 gtatacgcgc cggcaccggc gatatagttg gctcctgcca gatcgcccgt ggtctgggca
     2401 ttacctgcac gccacaaacg tttgtcgaac aggttgtcca cgccgccggt cagactgaca
     2461 ttcttcgtca catcccaggt cgcgctcaga cccacaatgc tgtaaggact gatttctttg
     2521 gtttccgggc caaccgctgg ctgaccttta tagttgtact tcttcggctg ctgcttgccg
     2581 taccaggtga aggtcgtttg catcgacaaa tcttcccgtg cctgccagct cagcgttgag
     2641 ttcaacgtgt actccgggat gatcgacaaa cggtcgcccg tggttttgtt ttcactcttc
     2701 agcatataag tgatgttatt ggtccacatc accgtttcgc taactggtac gtttaacgat
     2761 ccttccagac cttcaaccac tgctttcggc acgttatccc actgatagag atcagtgccg
     2821 actgcgtttt gccctacagc cacatagccc gcttcaatct tattgcgata atcgttacgg
     2881 aaccaggtga cgcccgccag ccagccgtcg cgtttgaact ccagaccaat ctctttgttg
     2941 atgctggttt ctactttcag gtcatcgtta ccttgcagat agcagccgcc cgcgctggca
     3001 tagcagccct gacctttact gtagagaatg tagttcgggt tagtctggta taggctcggc
     3061 gctttatagg cgcgggcaat gcctattttc agcgtgaagt catcgcctaa accttgcgat
     3121 atgttcagcg ccgggctcca gttattgccg acaatactgt gatgatcgaa acgcagcccc
     3181 ggcgttacga tggtgctgtc agtcagctcc atgttgtttt cggcaaacag cgagaaaatt
     3241 tctgcttttg aatacgggct acggtcggtg gcactcacgc catcaatagc gccaccggta
     3301 ttcgttccgg tcagtgcttg ggtgttggaa cttaagtcct tcatccgttg ctgattccac
     3361 tccgtaccca gcgtcagcgt ctggttaacg aggaaatcaa tcggcaggtt aacttcgctg
     3421 tgcagcatca cgtcatcgag atcgatatcg acgaaatcct gtgtcgcttt ttcgttaaat
     3481 ttcccttcgg taccgcccgc cagaccttcc ggaatacgcg agttacgggt gtgttcatac
     3541 tgcacccagt tgctggtggt cacgccgtta tcccagccac cgttccaggt cagcgcgtag
     3601 ttctggcgat acaggcggtt ggtttcatcg ccatatttcg agcgagtgta agcgtcagag
     3661 ttggtgttct gggtatcgcc cgcatacagg ttaccctggc ggctgtaacc cgcttccagt
     3721 tccagcgatt gcaatggtgc gaagtcccag cgcaccacgc cgttaatatc tttattgatc
     3781 accccttcgc gcccggctgg taacgtcgtg gcatacgttc cggcacgcgc ggactgatgg
     3841 ccctggttga tatcccacgc gtcagcctga gttttgtcga ggttgccata caagcggaag
     3901 ctgaattcgt cgcccagcgg accggtcagg ctaaagttag tgcgtttggt ggcaccttcc
     3961 tctttatgtt ctggcgcatt gaaatatgcg tcccaggagc cgtgccactc gccgctgcct
     4021 tttttggtaa tgatgttaac cacgccgccc gccgcgccgt tgccataacg cgcagctgcc
     4081 ggaccacgca gaacttcaat acgttcaatc atttcaggtg gcacccagga ggtatcacca
     4141 cgggtatcgc gctcgccacg ccagccctga cgcaccgagt tacggctgct taccggcttg
     4201 ccgtcaatca aaatcagcgt gttttccgga cccataccgc gaatatcaat ctgtcggtta
     4261 ttcccacgct gaccactggt ggagttaccg gtcaggttaa cgcctggcat ggtacggatg
     4321 atctccgaca catcgcgggc aaccgggttt ttgcggattt catctgcggt gatggtcgaa
     4381 acgccaggag cctgcaagtt ctgctcggcg gcggtaacga caatggtatc gtcatgtgaa
     4441 acaggtgtat cggtcggctc ttgtgcctgc gctaccccat aaatccccag attgaccaac
     4501 aaggccaggg aatgaatctt cttgttcatt gttttattcc tgcatttttg ccacgaattg
     4561 caactgtcgg gcatggtcgt catcaacacg acgcatcccg ttaccgcgaa aacctttgat
     4621 cctgaaagac acgcagtgca gttggttaat taatgtccgc gcttcccacg gcgcgccatt
     4681 acgctattgc aaatgcaaat agttattaat aacattatca atatatttct gcaatcaatg
     4741 aaaaattgca cagtaaacat ggggttatgg tgtgacggcg ttaaaagtag gaagtgagag
     4801 ctggtggcag tcgaaacatg gcccggaatg gcagcgtctg aatgacgaaa tgtttgaggt
     4861 cactttctgg tggcgtgatc cccaaggttc tgaagaatac tcgacgataa agcgcgtatg
     4921 ggtctacatc actggtgtga ccgatcatca tcagaacagc cagccccagt cgatgcagcg
     4981 aattgcaggt actgatgtct ggcagtggac gacacaactc aatgccaact ggcgcggcag
     5041 ctactgcttt attcccaccg aacgcgatga cattttttct gcaccatccc ccgatcgcct
     5101 cgaattacgt gaaggctggc gaaaactatt accccaggca atagccgatc cgctgaatcc
     5161 acaaagctgg aaaggcgggc tagggcacgc tgtttctgca ctcgaaatgc cgcaagcgcc
     5221 tctgcaaccg ggatgggatt gtccgcaagc gccagaaata ccagccaaag aaattatctg
     5281 gaaaagtgaa cggttgaaaa attcacgtcg tgtatggatt tttaccaccg gcgatgtaac
     5341 agcagaagaa cgcccgctgg cagttttgct cgatggcgaa ttttgggcgc aaagtatgcc
     5401 cgtctggcct gtgctgactt cgctgaccca tcgtcagcaa cttcctcccg ccgtgtatgt
     5461 gttgatcgac gctatcgaca ccacgcaccg cgcccacgaa ctgccgtgta atgcggattt
     5521 ctggctggca gtacagcaag agttattacc cctggtgaaa gttattgccc cttttagcga
     5581 tcgcgccgat cgcaccgtgg tcgccgggca gagttttggt gggctgtccg cgctgtatgc
     5641 cggactgcac tggcctgaac gctttggctg tgtattaagc cagtcaggat cgtactggtg
     5701 gccgcatcgg ggcgggcagc aagagggcgt gttacttgaa aagctaaaag ctggtgaagt
     5761 tagcgccgaa ggtctgcgca ttgtgctgga agcgggtatt cgcgagccga tgatcatgcg
     5821 ggccaatcag gcgctgtatg cgcaattaca ccctataaaa gaatccattt tctggcgtca
     5881 ggttgacggc ggacatgatg cgctttgttg gcgcggtggc ttgatgcagg ggttaatcga
     5941 cctctggcaa ccacttttcc atgacaggag ttgaatatgg cattcagtaa tcccttcgat
     6001 gatccacagg gagcgtttta cattttgcgc aatgcgcagg ggcaattcag tctgtggccg
     6061 caacaatgcg ccttaccggc aggctgggac attgtgtgtc agccgcagtc acaagagtcc
     6121 tgccagcagt ggcttgaagc ccactggcgt actctgacac cggcgaattt cacccagttg
     6181 caggaggcac aatgagccag catttacctt tggtcgccgc acagcccggc atctggatgg
     6241 cagaaaaact gtcggattta ccctccgcct ggagcgtggc gcattacgtt gagttaaccg
     6301 gagaggttga tgcgccatta ctggcccgcg cggtggttgc cggattagcg caagcagata
     6361 ctttgcggat gcgttttacg gaagataacg gcgaagtctg gcaatgggtc gatgatgcgc
     6421 tgatattcga actgccagaa attatcgacc tgcgaaccaa tattgatccg cacggtactg
     6481 cgcaggcatt aatgctggcg gatttgcaac aagatttgcg cgtcgatagc ggtaaaccac
     6541 tggtctttca tcaactgata caggtggcag ataaccgctg gtactggtat cagcgttatc
     6601 accatttact ggtcgatggc ttcagttttc cggccattac ccgccagatc gccaacattt
     6661 actgcgcatt gctgcgtggc gaaccaacgc ctgcttcgcc gtttacgcct ttcgctgatg
     6721 tagtggaaga gtaccagcaa taccgcgaaa gcgaagcctg gcagcgtgat gcggcattct
     6781 gggtggaaca gcgtcgccaa ctgccaccgc ccgcgtcact ttctccggca cctttagcgg
     6841 ggcgcagcgc ctcggcagat attttgcgtc tgaaaatgga atttaccgac ggggaattcc
     6901 gccagctggc tacgcaactt tcaggtgtgc agcgtaccga tttagccctt gcgctgacgg
     6961 ctttgtggct ggggcgattg tgcaatcgca tggactacgc cgccggattt atctttatgc
     7021 gtcgactggg ctcggcggcg ctgacggcta ccggacccgt gctcaacgtt ttgccgctgg
     7081 gtattcatat tgcggcgcaa gaaacgctgc cggaactggc aacccgactg gcaacccgac
     7141 tggcagcaca actgaaaaaa atgcgtcgtc atcaacgtta cgatgccgaa caaattgtcc
     7201 gtgacagcgg gcgagcggca ggtgatgaac cgctgtttgg tccggtactc aatattaagg
     7261 tatttgatta ccaactggat attcctggtg ttcaggcgca aacccatacc ctggcaaccg
     7321 gtccggttaa tgaccttgaa ctggccctgt tcccggatga acacggtgat ttgagtattg
     7381 agatcctcgc caataaacag cgttacgatg agccaacgtt aatccagcat gctgaacgcc
     7441 tgaaaatgct gattgcccag ttcgccgcgg atccggcgct gttgtgcggc gatgtcgata
     7501 ttatgctgcc aggtgagtat gcgcagctgg cgcagatcaa cgccactcag gttgagattc
     7561 cagaaaccac gctgagcgcg ctggtggcag aacaagcggc aaaaacaccg gatgctccgg
     7621 cgctggcaga tgcgcgttac ctgttcagct atcgggaaat gcacgagcag gtggtggcgc
     7681 tggcgaatct gctgcgtgag cgcggcgtta aaccagggga cagcgtggcg gtggcactac
     7741 cgcgctcggt ctttttgacc ctggcactcc atgcaatagt tgaagctggc gcggcctggc
     7801 taccgctgga taccggctat ccggacgatc gcctgaaaat gatgctggaa gatgcgcgtc
     7861 cgtcgctgtt aattaccacc gacgatcaac tgccgcgctt tagcgatgtt cccaatttaa
     7921 ccaacctttg ctataacgcc ccgcttaccc cccagggcag tgcgccgctg caactttcac
     7981 aaccgcacca cacggcttat atcatcttta cctctggctc caccggcagg ccgaaagggg
     8041 taatggtcgg gcagacggct atcgtcaacc gcctgttgtg gattcaaaat cattatccgc
     8101 tcacaggcga agatgtcgtt gcccaaaaaa cgccgtgcag ttttgatgtc tcggtgtggg
     8161 agtttttctg gccgtttatt gccggggcta aactggtgat ggctgaaccg gaagcgcacc
     8221 gcgacccgct cgctatgcag caattctttg ccgaatatgg cgtaacgacc acgcactttg
     8281 tgccgtcgat gctggcagca tttgttgcct cgctgactcc gcaaaccgct cgccagagtt
     8341 gcgtgacgtt gaaacaggtt ttctgtagtg gtgaggcctt accggctgat ttatgccgcg
     8401 aatggcaaca gttaaccggc gcgccgttgc ataacctgta tggcccgacg gaagcggcgg
     8461 tagatgtcag ctggtatccg gcttttggcg aggaactggc acaggtgcgc ggcagcagtg
     8521 tgccgattgg ttatccggta tggaatacgg gtctgcgtat tcttgatgcg atgatgcatc
     8581 cggtgccgcc gggtgtggcg ggtgatctct atctcaccgg tattcaactg gcgcaggggt
     8641 atcttggacg acccgatctg accgccagcc gctttattgc cgatcctttt gctcctggtg
     8701 aacggatgta tcgtaccgga gacgttgccc gctggctgga taacggcgcg gtggagtacc
     8761 tcgggcgcag tgacgatcag ctaaaaattc gcgggcagcg tatcgaactg ggcgaaatcg
     8821 atcgcgtgat gcaggcgctg ccggatgtca aacaagccgt tacccacgcc tgtgtgatta
     8881 accaggcggc agccactggt ggtgatgcgc gtcagttggt gggctatctg gtatcgcaat
     8941 cgggcctgcc gttggatacc agcgcattgc aggcgcagct tcgcgaaaca ttgccgccgc
     9001 atatggtgcc agtcgttctg ctgcaactgc cgcagttgcc acttagcgcc aacggcaagc
     9061 tggatcgcaa agccttaccg ttgcctgaac tgaaggcaca agcgccaggg cgtgcgccga
     9121 aagcgggcag tgaaacgatt atcgccgcgg cattcgcgtc gttgctgggt tgtgacgtgc
     9181 aggatgccga tgctgatttc tttgcgctgg gcggtcattc gctactggca atgaaactgg
     9241 cagcgcagtt aagtcgacag tttgctcgtc aggtgacgcc ggggcaggtg atggtcgcgt
     9301 caaccgtcgc caaactggca acgattattg atggtgaaga ggacagctcc cggcgcatgg
     9361 gattcgaaac cattctgccg ttgcgtgaag gtaatggccc gacgctgttt tgtttccatc
     9421 cggcgtccgg ttttgcctgg cagtttagcg tgctctcgcg ttatctcgat ccacaatggt
     9481 cgattattgg cattcagtcg ccgcgccctc atggacccat gcagacagcg acaaacctgg
     9541 atgaagtctg cgaagcgcat ctggcaacgc tacttgagca acaaccgcac ggcattgcgg
     9601 cgcggctgcg tgcccgtggc gaacaggtgg catttcttgg tttgctggat acctggccgc
     9661 cagagacgca aaactggcag gaaaaagagg ctaatggtct ggacccggaa gtgctggcgg
     9721 agattaaccg cgaacgcgag gccttcctgg cggcacagca gggaagtact tcaacggagt
     9781 tgtttaccac cattgaaggc aactacgctg atgctgtgcg cctgctgacg actgctcata
     9841 gcgtaccgtt tgatggtaaa gcgacgctgt ttgttgctga acgcacgctt caggaaggta
     9901 tgagccccga acgcgcctgg tcgccgtgga ttgcggagct ggatatctat cgtcaggatt
     9961 gtgcgcatgt ggatattatc tctccagggg cgtttgtaaa aattgggccg attattcgcg
    10021 caacgctaaa caggtaaatt aatattatct ataaaccca
//
LOCUS       DAZSNO010000137        10027 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZSNO010000137 DAZSNO010000000
VERSION     DAZSNO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.137"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHWN2_003199"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHWN2_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFR8932629.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="ACHWN2_003200"
     CDS             complement(1433..1759)
                     /locus_tag="ACHWN2_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFR8932630.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="ACHWN2_003201"
     CDS             1894..2235
                     /locus_tag="ACHWN2_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFR8932631.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="ACHWN2_003202"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="ACHWN2_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="ACHWN2_003203"
     CDS             complement(2833..3543)
                     /locus_tag="ACHWN2_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFR8932632.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="ACHWN2_003204"
     CDS             3651..3956
                     /locus_tag="ACHWN2_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFR8932633.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..5568
                     /locus_tag="ACHWN2_003205"
                     /pseudo
     CDS             4155..5568
                     /locus_tag="ACHWN2_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(6295..7284)
                     /locus_tag="ACHWN2_003206"
     CDS             complement(6295..7284)
                     /locus_tag="ACHWN2_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932634.1"
                     /translation="MTMIKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTVKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7361..7852)
                     /locus_tag="ACHWN2_003207"
     CDS             complement(7361..7852)
                     /locus_tag="ACHWN2_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932635.1"
                     /translation="MLFNNNDWKLSVTDINLYENTVSLDGQSYPLSFAIKTLIPGYLS
                     GLPATSRESMELLEALAEAGVAIGNFFSNDLMTAYQRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQRELQRREQVKAERRTYGENLHSATHSAGRSRAAIVADIESGGNWM
                     DSL"
     gene            complement(7965..8168)
                     /locus_tag="ACHWN2_003208"
     CDS             complement(7965..8168)
                     /locus_tag="ACHWN2_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932636.1"
                     /translation="MAMIDPRTPIGKATLRYRGLPTRHLLSLLRLGVEDPERPFYSRD
                     ELIAMLVDRDLNNQLRRAFAKQS"
     gene            complement(8171..8668)
                     /locus_tag="ACHWN2_003209"
     CDS             complement(8171..8668)
                     /locus_tag="ACHWN2_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932637.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDTE
                     STQYKRFAQTKVGKLLRNRQKIRLIRMGELGSTVLADPAATVEFGRWLDFEYGLAVSE
                     AFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTAGR
                     CEWGK"
     gene            complement(8658..9173)
                     /locus_tag="ACHWN2_003210"
     CDS             complement(8658..9173)
                     /locus_tag="ACHWN2_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932638.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDPEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDTERLQAKADQEQRFKSFA
                     EALAGGASYDF"
BASE COUNT         2560 a         2535 c         2507 g         2425 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaaccctg cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccggat cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaacttcaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggacccccg gtggcctcgg
     5701 tctggacttg aggtgggcat atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtctgcggaa gtcatggaca taaagcctcc ttcgtctatt gtactactgc actagataac
     6001 tagtacaaaa caaaggtcca gaccattggt ccagaccata gttagttaat cacagtgata
     6061 gcccaccacc tgaagtcctg ctgatattca gtccagactt acctctgctc atggtcttac
     6121 ctccctcggt ctcctgacct tgggtatcga ctacagcttg ctgagcgtca tctggaggag
     6181 ctacaggagg agctacagga ggcgggaccg aagccccacc ttgagtccca ccaccacctg
     6241 tcaggccctt ggcgatggac ctgaagcctt tactaatgct cttacccatt ggtcttacgc
     6301 ttcggtcttg gtgaacttca ggcgtttgac tacaggcagg acggcagtag ctgagccagt
     6361 gcccatcttg acggtcgtag cagcttcagc gacaacgaac actttaccga ggatttccaa
     6421 gtcgtgggac tgagagtcgc catcacgtga aaggacgacc gtaatattct caccgtcgtg
     6481 acggaagcag ccgaagctca gggccgtgaa gcgtttagcc agcatgaaca cacctttgtc
     6541 ctcaccgttg taatacgcga tgtcggtacc cagcatatcc acgatagcgt cttcaccaga
     6601 gaagccctgt ttagcagcca gacgggacag cgctttcagt tgggactcat gggcgatgat
     6661 gccgtaatca gacacttcag agccgaaaga ggactcggtg ttctcaaaca gagcgtccat
     6721 gatagcgtct gccttagtgg tcaggcttcc agtcaggact ggcagctcta cggtcttcag
     6781 cagcttggag gactccagaa cgtcaaccat gcgagcgttg atggtacgga ccacattctc
     6841 acagatgttc atagcgtcct gctggagtgt gccatgagtg aaaccagtgg aggacttggc
     6901 gtcccacagt ttagtttgct ccgagatgag gattgcctgt tcgataggtt cgacgacaat
     6961 ctcctgacca gacggaacgg tcacgacttc aggtcgctca ccaccgcgta cagggacgac
     7021 cacagagcgg tcagacagag tgtgggcgat gattggggta ggcaggctat tgcgggtagg
     7081 caggacttct acagggaacg gaatgttatc ctgagcgtct ttaagtacac cgttcagcat
     7141 agccttaacc gtcttagcta actggagggc atagacctgt gggtcagcgt tagttggcag
     7201 accttcgtta acctgaatgt tgtcggtgtc ggtgatacgt actggctcaa tgccgaactg
     7261 atagctatct ttaatcatgg tcataataat caatctccta aattagtttt agatactgtg
     7321 ggtcccgaca tcaggtcagg accacttgtg tgtctgtgtc ttagaggctg tccatccagt
     7381 taccgcctga ctcaatgtca gcgacaatag cggcacggga gcgaccagcg gaatgggtag
     7441 cactgtggag gttctcacca taggtccggc gctcagcttt gacctgttcg cggcgctgta
     7501 gctcccgctg ccactctgcg tcggtcatgt tctcttcacg catacgctcg gcctgaagtc
     7561 gttgctcttt agcgatacgt tcagcctcag ctcgtttgtt catctggcgg cgttggtagg
     7621 cggtcatcag gtcattactg aagaagttgc caatagccac accagcttca gccagagctt
     7681 ccagtaattc catggactcg cggctggtgg caggcagtcc tgacaggtag cccggaatca
     7741 gggtcttaat agcgaaggac agtgggtagg actgaccgtc cagactcaca gtgttttcgt
     7801 agagattgat gtcggtgact gacagtttcc agtcgttgtt gttgaataac ataaattggt
     7861 actcctcttg agagttgaga ctatcggtat cgcagacgca gtagcgccta cgggtctccc
     7921 cgcatcggtc atctcaaaag gtgtttaaat taggtatggt atggtcagga ctgcttagca
     7981 aacgctcggc gtaactgatt attcaaatca cggtccacca gcatggctat cagctcgtct
     8041 cgactgtaga aaggacgctc ggggtcttcg actccaagtc tcagcagaga caggaggtga
     8101 cgtgtaggca gaccacggta acgaagggtc gctttgccga taggtgtgcg agggtcaatc
     8161 atagccatga ttacttaccc cactcacagc gaccagcggt cacagcttga tagtctgaat
     8221 actcagcctg tgagacttca cgtccggcga tgtagaggcc tgtgccatga cgctggatga
     8281 attccttggt ctccggcttg atgtcctcag cagacgctac agcagactgc acggtggcgc
     8341 tggaggtcat agacacgaag gcttcagaga ctgccagtcc gtactcaaag tccagccagc
     8401 gaccaaactc tacagtagcg gcggggtcag caaggacggt agacccgagt tcacccattc
     8461 ggataaggcg gattttctgc ctattcctca gtagctttcc aactttggtc tgagcaaaac
     8521 gcttgtactg tgtcgactcg gtgtcttcta ctttgccaat ctgaatgccg acccggcgaa
     8581 tttcatcgag tcgaaacaat ccgtcctgac tggtgatgtc ctgaccgaag atatgaatgg
     8641 tgtattcctg cttattgtca gaagtcataa gaggcacctc ctgccagagc ttcggcaaag
     8701 gacttgaagc gctgctcttg gtcagctttc gcctgaagac gttcggtatc aggtgggacc
     8761 agcttatcaa aggtgccgtt ttcgtaatcc tcagttagga cctgaagtcg gcgggaccat
     8821 ttgctctcca cctgcatcat cgcgttagag atgtgcttca agtcggcatc agagcgtgac
     8881 gctatccaat ccatctcttc tgggtcaccc tcacaggtgt aaggattgtt gaaatagaag
     8941 atgtcctgta ggccacgttt cagctcttcc tcggtgtgtg tctggaggta tgactggata
     9001 ggctccgcgt acatctcccg gattttctca tagtcccagt cttccgcttg ggcataccat
     9061 cgggataact tcagcaggtc taagttatag cgacagcaat cgacactgga ctctgcattg
     9121 gtcagcagga tgattaagaa ctggcggtta gttactggta attcggtatt catataaagg
     9181 atgctccttt ggtaaagcac tttctgtctc cacgtggagt ccgcacctat tcaatgcatc
     9241 caaacggacc caccaaaaag tgcttaacga aagtgaatag gattaaggat ggtctggagt
     9301 gacccggtgt ctctacatgt acgtataaag gcgagaccgc atgtcaggac gagctgtaag
     9361 gaccccagat ggtgagcggt gatagactta agccaatgta gtgagtagac gagctactaa
     9421 ctgagatgac tgaagaacgc taacgagctg gaggtcatac aggaggtctg acagaggcgg
     9481 tcacagacct tgactactta ggagagatac ttaatgtcgg ttgaccagat gctcgcatgt
     9541 tctgacgcat cagactcaga ggcaaggttg gtctccctat agtgatagtt taggggggtg
     9601 tccctatagt gagggggtat tccagcccag agctggcgcg gctctcagga caggcactta
     9661 tcgttgtagt cccaccatgt gaaatcccct cggtccttca gaaccttagc aagactcaca
     9721 gccgtagcct gcaagacgct attaacgtgg cgcaggtagc gaggcgaagg tgagcgaccc
     9781 gtgatggcgg tgtatgactt ggcagtgttg cgggagaaga tgtcgtttgt gccgtataag
     9841 gccgcgaaca ggtacgcctt ggataccggg agtccagcac cgatagagcc actggtagcc
     9901 cgtgagagtc ctgtgaacac atcgtcagcc cacgggtacg cctcaacaag ctgcgtatag
     9961 gagagacacc cagtctcaat cagccacgca cggacagccc ggttgtcgat gaccacagga
    10021 ggcttac
//
LOCUS       DAZSNO010000138        10010 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZSNO010000138 DAZSNO010000000
VERSION     DAZSNO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10010
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.138"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..337
                     /locus_tag="ACHWN2_003211"
                     /pseudo
     CDS             1..337
                     /locus_tag="ACHWN2_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            complement(423..986)
                     /locus_tag="ACHWN2_003212"
     CDS             complement(423..986)
                     /locus_tag="ACHWN2_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR8932639.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(1033..2394)
                     /locus_tag="ACHWN2_003213"
     CDS             complement(1033..2394)
                     /locus_tag="ACHWN2_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFR8932640.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(2446..2676)
                     /locus_tag="ACHWN2_003214"
     CDS             complement(2446..2676)
                     /locus_tag="ACHWN2_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932641.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(2900..3019)
                     /locus_tag="ACHWN2_003215"
                     /pseudo
     CDS             complement(2900..3019)
                     /locus_tag="ACHWN2_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332787.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(3714..3905)
                     /locus_tag="ACHWN2_003216"
     CDS             complement(3714..3905)
                     /locus_tag="ACHWN2_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932642.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(3902..4324)
                     /locus_tag="ACHWN2_003217"
     CDS             complement(3902..4324)
                     /locus_tag="ACHWN2_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR8932643.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(4371..4796)
                     /locus_tag="ACHWN2_003218"
     CDS             complement(4371..4796)
                     /locus_tag="ACHWN2_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR8932644.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(5214..5990)
                     /locus_tag="ACHWN2_003219"
     CDS             complement(5214..5990)
                     /locus_tag="ACHWN2_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932645.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(6041..6475)
                     /locus_tag="ACHWN2_003220"
     CDS             complement(6041..6475)
                     /locus_tag="ACHWN2_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR8932646.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(6489..6710)
                     /locus_tag="ACHWN2_003221"
     CDS             complement(6489..6710)
                     /locus_tag="ACHWN2_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932647.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(6711..7394)
                     /locus_tag="ACHWN2_003222"
     CDS             complement(6711..7394)
                     /locus_tag="ACHWN2_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR8932648.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(7471..7764)
                     /locus_tag="ACHWN2_003223"
     CDS             complement(7471..7764)
                     /locus_tag="ACHWN2_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932649.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            7911..8873
                     /gene="parM"
                     /locus_tag="ACHWN2_003224"
                     /gene_synonym="stbA"
     CDS             7911..8873
                     /gene="parM"
                     /locus_tag="ACHWN2_003224"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR8932650.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            8876..9226
                     /locus_tag="ACHWN2_003225"
     CDS             8876..9226
                     /locus_tag="ACHWN2_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR8932651.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(9349..9630)
                     /locus_tag="ACHWN2_003226"
     CDS             complement(9349..9630)
                     /locus_tag="ACHWN2_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932652.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(9758..9992)
                     /locus_tag="ACHWN2_003227"
                     /pseudo
     CDS             complement(9758..9992)
                     /locus_tag="ACHWN2_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637348.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT         2357 a         2809 c         2605 g         2239 t
ORIGIN      
        1 gagcgcaggc cacggcgcga caagggtggc gcgaccgttc cggtcgctgc aaacccgggt
       61 ttttgcgtca gggatggaag cccgccaggg cggagacagc tccgctggct ccgttcacga
      121 cagcccgccc cgccaggggg acgcccggaa aaggcataag caggagaaca acaaaacaga
      181 cagtaatcag agcagcagaa aaaagaagaa cagtagcagt agcagtagca gtagcagtag
      241 caggaacaac aacatcatca gtaacaggca aatgacaggt ctgaaaagac agaagcccac
      301 agcagaacgt ccgcgtaatg aaaggggcgg cgcgtaagcg cggccccttt cagcgccacc
      361 ggcgcgcggt cttttcccgg cgcgggcaga cgggcattta tcctcccgcc tgcccggatt
      421 catcaggcac gcagacgaat aatcatcgtc ggaacacggg tataagcaaa tgtgccgcac
      481 ggcagcacct cgcggacatc agaaaacggt aacagcttct cctgctggcg cggcccgttc
      541 agacagacgg caaccagaac gccccccgga cgcaacaggg aaaaagcccg caggatatgc
      601 cggatatcct gcccgtgact gaacggcgga ttcataatga tccggctgta atactgcacc
      661 ggctgccatt ccatgaagtc gccacactgt accctgacac cgttaaagtt ttcccgcaga
      721 taccggacca gcccgctgtt aatctccacc gcatcacaca tggcctccgg cgcggtatcc
      781 cgaatcgccc gcagtatggc cccggtgccg gcagagggtt ccagaatgtg atcacggttg
      841 ctgatttccg ccagcgtcac caggcgatca cagaccgggg caggagtcac aaaaagctga
      901 gattcagccc ggatttcagc aaccggcgcg gcgttatcca gccttgcgcg catcagccgg
      961 atacgttctc ttaactgact gtgcatgtca ttatctccgt catcccccgg ggatttcccg
     1021 gggggatata ccttatttct gcgggatttc cgtgattttc atgtcggtga tatacacatt
     1081 caccagacgg aaattattgt ccattgtgcg gcggtagcgg tatgccccgt ggtcttctgc
     1141 ttcttccaca ctgcgcacgg ccttacagtc ccggggcagg gcggcccact gcgcctttgt
     1201 catttcccgg aaaccttccc ccggatagtt gactaccggc ggacgcttcg cggcctgact
     1261 ggcgacggca gcctcttctg ccgatggtgc tttgtagtcc gtgatgcgat cgggcgtcac
     1321 tttcatcgtg ccgctgtacc cgagaaaact gtaatttggc gttgtgactg aactcaccgc
     1381 cccgttgctt ttgttcacgc ggatgatagt cagccactcg ccccggctga aaacctgtcc
     1441 gcccggctca aaatcctgtg tccgggtaac cacgccgccg ctttcgtcca gcatggcacg
     1501 ctcatagatc aggcggttct gatagtggtt aacccagcgt tgctgatgct gaatctgccg
     1561 ttcatgacag cggatcgcaa tttcgcgggc ctgctccgtg gtgatgatgt catcatccag
     1621 ggccgaccat aacgaccggc tcccttcata ctggcttttt tctgccgggc gcggatacgt
     1681 atccagcggg aaacaggcgc tgatatggtc atgactgctg atgagttttg ccatattcag
     1741 atccagcgat tcagcccgcc acatcgtcag atatttctgc gactgtgcga tggtcttttc
     1801 agccttgcgc aaatccgctt cgattttttt gatacgacgc cagcgaacgt ccgggcgttc
     1861 tttatacttc gcgtgaagca gtgccgaccg cgcccgctcc tcccagtatt ccgcacgttc
     1921 aaagagcatc acggcacgtt tcatgccgtt ttcaatacgc tgcgcatcac gacgggcgcg
     1981 gcgttcgctg tgatgcccca caagaatggg ctgaccgggc gggatcatcg cggccagtct
     2041 ttccacttca tcaagtgcct gtgcggattc actggcccgc tttccgctgt atccggtaaa
     2101 ccgctccgcc cgtgcttcct gacgttcagc gagcgtgctg tcttcatcct caatctctcc
     2161 ggcaagtgag aggagcacgt cttcccggcc cggtgtccag gccggcgcga caaacagcgc
     2221 ctgtttgggt gcccagcgaa aaccggcatc atgcactttt ttatacgtct ccgggtcaag
     2281 acgtgacgcg gcatacaggc gcagtttgtt gtcatccggt gagtaggtcg cgcggtacat
     2341 ctgcacaggg ctggcggctt ccggggcggt catggtgatt gctgctaatg tcataaaatg
     2401 ttctccttcc gtctgttgcc gcccctcgcg gggcggctca cctcatcagt taaaagcgcg
     2461 tttcagttcg agtgtgcgga gcacctgccc gaaagccgtg tttttcatgg cggggatgtg
     2521 tacaccacgc ccccggtgaa aactccgggc tatgtgtcgc ccgaaggctt tcaacacggg
     2581 gtcaatttcc ggcgtgggga tgtcttcgcg tctgaaacgg ggatgacggc ggggctttac
     2641 agccgctgcg gaaggtgaaa caatgcttac agtcataaat ggattcctta tgacaaattg
     2701 gttaacaatg ccgtcaaaat tggtcgcaca attcttacgg cgtggattaa gcaggtgcct
     2761 gacctgcttt tttcatatct gcatctgacg ctttccggtg ttttcctcct tctgttcctg
     2821 cgttctcatt acctgtcagc cggatgactg acagaacaaa taaatgcgta ttgccggagc
     2881 ctgaaataaa tcctgatttt taaagaacat cacccgttga gtttcgggag ccgcggtttt
     2941 gacgtttcgc gggaacctgc tgctgtattc actgtggtat gaacttcacc cgtttgagaa
     3001 ccttcgccgc aaggggcatc actgccacgg cctgacgcac ctgtcctggg cagaacgcaa
     3061 aattttttac cgggttttgg gtaaaaaatt ttgtgggctg ttcatttacc ctggacagtc
     3121 agtgcggcag gttgtggctg tatcagcccc cggcgacagg gactcatacg ggtgtgtggc
     3181 acccggcagg caatgagcag gggcccgccg caacgacgga gagcgcgtgc gcgaaacggc
     3241 gttgcccctt acatcccggc ggccttttgc cgggatgccg ggagcgcagg ccacggcgcg
     3301 acagaggcgg cgcgaccgga acggtcgctg caagccaggg gttttcacgc atcggaccgt
     3361 agcggcagcc cgaaaccgcg ccagcgggtt cggcggagac tggcaggtgc gccagcagct
     3421 gcctgtcgga gtcgggcctg acgaccggat gccgccaggc agccgggagc cgaaggtgcg
     3481 cccggcagac agaacaacac tgcagtagtc agaatcagag cagtagataa aggaacaaca
     3541 acatcaggaa cagttccata acataagaaa gaaacactcc tgagtcagac acatcacaga
     3601 cgggaagggt aacgggcgac gcgccagcgt ggcccgcttc ccttccggca ggcgtcagcc
     3661 tgccaggggt tttcgcgcgg gctgacaggc ggcctccacc gcctgcccct gtatcacctg
     3721 tttatgctgc cctgaaggat gtagtgaccg ccgaggaata cagcctgctg attcgccaca
     3781 aaatcgcaga tttcatcaac aatctgcgca gtggtgagcg gtttctgccc catttcacga
     3841 cgccgcgcat taatcacggc tttgtaattg cgcagaattt cgcgggtttc tgtgctgata
     3901 ttcatgacat gtgctccccg tccagcgcct gtaacacaac gtccattgct tcgcgttctt
     3961 ccctgacgaa tgcgtcaccg tcgcccccgt tctcgctccc gacggcatac aggcgcttca
     4021 tttcactgcc tgccagcgcc atcacttttt ccagcatcac cgccggaact gtcacctgac
     4081 ggctggcgcg cttttcttcc atcagctcac gaacaacgtc gtgaacaatg ctgacatccg
     4141 caccatgcgc aaaggcatcg tccagtcgct gcatgacggt ttccagctca tcgtctgtca
     4201 gactccagtc ctgggctatg tgacgcacat ccccacgggt cagcaccacg gcaaaaacgc
     4261 attcactgtc cggcacgtct gtgttcagca cttcgcggat aatttcttta acagtacagt
     4321 acatgttctc gtctccttta caggggcggc attgccgccc cgtccgttaa tcagtccagt
     4381 gccgcgtaaa tcgcgttgca ttcggggtga aattcgatgt gacgccacaa ctgcgcatcg
     4441 cgcatccggt aaagctgggt cagtcctgca tccccgctgt cgtggtacag ccacaactgg
     4501 cggttaatca caagggaagt cagaatgatg cctgcggcat cagcactcac ggtacggtca
     4561 aaccagtttg caccgttcac catgtggaaa cggtcgccat ccggcatcat gtaaccgcca
     4621 ccgtccggca ggcggatgaa gtgccagaag cctccctgat attccggcat cattttttgc
     4681 gccagcgcgt acacatacgc ctcggcatac agaaaatcgt tgccaaacag atacggtaga
     4741 aaggagagac ggtcgcactc ttcaacgttc agagtgacag gtttcgcata ttgcatgatg
     4801 ttttcctccg ttgttcacat attccgcgac accgggcggt gccgtgcctc cataaaagcc
     4861 gggcgataag gcggggatgc acagggcgca agggaagccg cagcctgacg cacacggctg
     4921 gcgggagttg gagggtgaga ccgcgaaatc tgagcgggtg aacgaccgga aaatcacggc
     4981 agacgggaag gagggaccgg gtgaccgcag cggcgaagcg tacccttgca tccctgccag
     5041 cccggctaag atggaggcac ggcacagccc gcacacgacg ctgaggccac ataactgaaa
     5101 aaccacaagg ccatgccctg catggccggg taaagcggag ccggcggcga cttcctttgt
     5161 gctctgtccg gggaccggca ggaaaaaggc ggggatttac cccgccaacg tcatcatttc
     5221 cggatttttg ccagggcatc cgccatgctg cggatttcgg cccgggcttc ccggaccacc
     5281 tcccgcagaa caccacggtc aaaccagcgg cggggcggtt catccggtat acgcagcaca
     5341 tcgcaggcca gcccactgga caggatatgc tcaatacaat cgctgaccag gtacatttcc
     5401 accgacgaaa aacgtgctcc ccggttcacg atggcatcga gcaccacggg caggttttcg
     5461 tcatcttcat cgagccagca ggggtgatcg ctttccttca caaccagacg aaagcggcgc
     5521 gtctggcgag tcagcaacgt ctgaccggtg gtggtcagcg ccgcccggat acgccgcgca
     5581 tcttctgcca cccggcaact gacactgcgt accgcatcgc gcaccgccgc aagtacagcc
     5641 ctgatttcgt cttcggtcag ccattctccg gcatacggga aagacacctg cgttccgtct
     5701 gccggaagta ccggcagcga ctgccactga ctgccggaca gtaccagttc cgccccggtt
     5761 ccggtcagtt tctgaatggc ggaggccagg agcggcggca gtacgccgcc gtgacggttg
     5821 ataagtaacg tcgccagccg gggcgcatcc agcagaacat aaatgctgat ccagcccgga
     5881 tacttcgggt tacgctggtc aacatccgtc tcttccggcc agccctgcgc cagcagcagg
     5941 ttgcgggcgt gacgacaaat cagtgcgttc agcgtgctgt tttcgttcat gattaagcgt
     6001 tctccctgta tcgccgcccg cagggcaggc ggcgataaca tcagtcgttt tcgtgtgtgt
     6061 tgtttttcag cagggtgcgg gcctgattaa ccacctcctg acggcgggtg acgcattccg
     6121 ggacggcgag gccatgatcc cgggcggccc attcacgttt ccacaatgcc tgttcagcgg
     6181 tctgaatcag ggacgccagc agttcagcag ggacggtcac caggcggttt tcgctctcgt
     6241 tgcggataat ctccatcacc gcagcggaag aaataccgga ctcaatccgc tggtcttccg
     6301 ggatgtcctc caggcgtgcc aggacggtcc tgatttcatg accggtcaga gaaatttcca
     6361 taccgtcagc cagtgcctga atctcttccg gggaccagat aaccagcatc agcggcatgt
     6421 ctcccggata ctttactgcc agctcccggc atagcgtttc gcatgttccg tacattctcg
     6481 tctccttctc aatctttaaa gcacgcttcc agttccagaa tttcggtata tgcctccatc
     6541 aggaggcggt ttgcgcgaaa cagcgtctga cgcaccagac gttccggcag ggtgtcggca
     6601 ttaaagctgt accgctgctc catctcattc atggttgtac gcaggtcgca catggcattg
     6661 gccacctccg ccacttcggc gcgcagtttt tcgtgtcctg catagttcat ttacgcagcc
     6721 tccggcataa accagtcatc attcgcggcc ccctgctgca tggcccgttg cacggcggca
     6781 aggcgttgct gcccggcacg gtgatactgc tcaagcagct cgataccgat ataccggcgt
     6841 ccggactgga gggcggcgac gcaggttgag ccgctgcctg caaacgggtc cagcacaatt
     6901 gcgttcgggt gtgtgaagct ctcaatcagc ggttgcaggc tggtaacggg cttttccgtc
     6961 gggtgatggc gattgcccga atatttccag cccagcacgt ccggcagcgg gttttgtggc
     7021 agacgtggac ggccttttgc caggatgtag gcacattcgt ggcgatagcc cacatatgcg
     7081 gccttcgatg tgtagttttt ggtgaagacc aggtgaccaa caacgctgaa tcccgcattt
     7141 ttccaggcgg ccataaagcg atcgacgcgg ttccagccat agaagctcac cattaacgcg
     7201 tcttttttga gtacgcggta catttcatta caggctggtt gcagccattc atcggtttta
     7261 tcgccggcga tggtgcgccc ggaacggtca cggaaaccga cgagatacgg cgggtcggtg
     7321 aggatgaaat cgacagcatt gcccggaaac gtggccataa cacgcacgca gtcacccagg
     7381 ataaaacggg acataacagc actccttctc ttacagaggg caccgggagg gcacccctgt
     7441 ggtgcccgtt ccccgctgcc tgtgggctgt tcaccgcgtc cggtaacggg tcaggccgac
     7501 cgtggaatac cgcagccgtc gtctttttga cggcgaggat atgccgcgaa agcggcttga
     7561 agggcgttca tcagaaaagg gcgacgggct ggcgtacact gtcaccaacg ccagccggga
     7621 gcggtaaaag cgcccgtccc gtgcccggcc ggacgcggga cactgaccac aggcagcgtt
     7681 cctgccgcgc cgcgtcagcc ggcgcagtaa aaagaggagc ccggcgacat cttttgttac
     7741 ccgccaaaca aaacccaaaa acaacccata cccaacccaa taaaacacca aaacaagaca
     7801 aataatcatt gattgatggt tgaaatgggg taaacttgac aaacaaaccc acttaaaacc
     7861 caaaacatac ccaaacacac accaaaaaaa caccataagg agttttataa atgttggtat
     7921 tcattgatga cggttcaaca aacatcaaac tacagtggca ggaaagcgac ggaacaatta
     7981 aacagcacat tagcccgaac agcttcaaac gcgagtgggc agtctctttt ggtgataaaa
     8041 aggtctttaa ctacacactg aacggcgaac agtattcatt tgatccaatc agcccggatg
     8101 ctgtagtcac aaccaatatc gcatggcaat acagcgacgt taatgtcgtt gcagtgcatc
     8161 acgccttact gaccagtggt ctgccggtaa gcgaagtgga tattgtttgc acacttcctc
     8221 tgacagagta ttacgacaga aataaccaac ccaatacgga aaatattgag cgtaagaaag
     8281 caaacttccg gaaaaaaatt acattaaatg gcggggatac attcacaata aaagatgtaa
     8341 aagtcatgcc tgaatctata ccggcaggtt atgaagttct acaagaactg gatgagttag
     8401 attctttatt aattatagat ctcgggggca ccacattaga tatttctcag gtaatgggga
     8461 aattatcggg gatcagtaaa atatacggag actcatctct tggtgtctct ctggttacat
     8521 ctgcagtaaa agatgccctt tctcttgcga gaacaaaagg aagtagctat cttgctgacg
     8581 atataatcat tcacagaaaa gataataact atctgaagca acgaattaat gatgagaaca
     8641 aaatatcaat agtcaccgaa gcaatgaatg aagcacttcg taaacttgag caacgtgtat
     8701 taaatacgct caatgaattt tctggttata ctcatgttat ggttataggc ggtggcgcag
     8761 aattaatatg cgatgcagta aaaaaacaca cacagattcg tgatgaacgt tttttcaaaa
     8821 ccaataactc tcaatatgat ttagttaacg gtatgtatct cataggtaat taatgatgga
     8881 caagcgcaga accattgcct tcaaactaaa tccagatgta aatcaaacag ataaaattgt
     8941 ttgtgataca ctggacagta tcccgcaagg ggaacgaagc cgccttaacc gggccgcact
     9001 gacggcaggt ctggccttat acagacaaga tccccggacc cctttccttt tatgtgagct
     9061 gctgacgaaa gaaaccacat tttcagatat cgtgaatata ttgagatcgc tatttccaaa
     9121 agagatggcc gattttaatt cttcaatagt cactcaatcc tcttcacaac aagagcaaaa
     9181 aagtgatgaa gagaccaaaa aaaatgcgat gaagctaata aattaattca attattattg
     9241 agttcccttt atccactatc aggctggata aagggaactc aatcaagtta ttttcttacc
     9301 agtcattaca taatcgttat tatgaaataa tcgtttgcac tgtctctgtt attcaggcaa
     9361 tttcaataaa ggcacttgct cacgctctgt cattttctga aactcttcat gctgcatttc
     9421 gatttttctt ctaaaatcat aacttgcata cctgttcttt ttatgtatat ccagatctat
     9481 ttttgtgacg agttcaaacc acaatgatag cactccccat gcagcgttat gaaacatttc
     9541 cgcaacagat ttagagtctg tattaaccgc ttcagtcatg tagtcgctaa tacgttctgc
     9601 agtgattttc gttatttcat cgtaattcat tttatcccct acaagactat ttaatggaat
     9661 gaaaaatatt tggtctgata acaatgctcc tgaattaaca cctgcaggct atgttaaagt
     9721 tgccctcaca cacaatctac ctgttttctc gtgtgtgtca caggcgcaat acataaatag
     9781 tctggccatt aagttcgtaa cggatttcgt actcgccagc aaaaatccgt ctgacatccc
     9841 tggtttcaaa ctgaaacaac tgttcatccc atacgtggat gagttaacag aatgaccggc
     9901 gcctgtgtca gggactacac cgttttggct gcagctggtt tactgaccaa cgccagaaaa
     9961 tcatataact gcgtcaatgt cagcgtatcc agcgcgactg tattattttt
//
LOCUS       DAZSNO010000139         9944 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZSNO010000139 DAZSNO010000000
VERSION     DAZSNO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9944
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.139"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            143..646
                     /locus_tag="ACHWN2_003228"
     CDS             143..646
                     /locus_tag="ACHWN2_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR8932653.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(726..977)
                     /gene="yecJ"
                     /locus_tag="ACHWN2_003229"
     CDS             complement(726..977)
                     /gene="yecJ"
                     /locus_tag="ACHWN2_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR8932654.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1092..1178)
                     /gene="azuC"
                     /locus_tag="ACHWN2_003230"
     CDS             complement(1092..1178)
                     /gene="azuC"
                     /locus_tag="ACHWN2_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR8932655.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1441..1764
                     /locus_tag="ACHWN2_003231"
     CDS             1441..1764
                     /locus_tag="ACHWN2_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR8932656.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1935..2432
                     /gene="ftnA"
                     /locus_tag="ACHWN2_003232"
     CDS             1935..2432
                     /gene="ftnA"
                     /locus_tag="ACHWN2_003232"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR8932657.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2469..2708)
                     /locus_tag="ACHWN2_003233"
     CDS             complement(2469..2708)
                     /locus_tag="ACHWN2_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR8932658.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2898..4109
                     /gene="tyrP"
                     /locus_tag="ACHWN2_003234"
     CDS             2898..4109
                     /gene="tyrP"
                     /locus_tag="ACHWN2_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR8932659.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4171..4836)
                     /gene="yecA"
                     /locus_tag="ACHWN2_003235"
     CDS             complement(4171..4836)
                     /gene="yecA"
                     /locus_tag="ACHWN2_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR8932660.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5032..5118)
                     /locus_tag="ACHWN2_003236"
     tRNA            complement(5032..5118)
                     /locus_tag="ACHWN2_003236"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5082..5084),aa:Leu,seq:taa)
     gene            complement(5131..5204)
                     /locus_tag="ACHWN2_003237"
     tRNA            complement(5131..5204)
                     /locus_tag="ACHWN2_003237"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5170..5172),aa:Cys,seq:gca)
     gene            complement(5259..5334)
                     /locus_tag="ACHWN2_003238"
     tRNA            complement(5259..5334)
                     /locus_tag="ACHWN2_003238"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5299..5301),aa:Gly,seq:gcc)
     gene            complement(5486..6034)
                     /gene="pgsA"
                     /locus_tag="ACHWN2_003239"
     CDS             complement(5486..6034)
                     /gene="pgsA"
                     /locus_tag="ACHWN2_003239"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR8932661.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6091..7923)
                     /gene="uvrC"
                     /locus_tag="ACHWN2_003240"
     CDS             complement(6091..7923)
                     /gene="uvrC"
                     /locus_tag="ACHWN2_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR8932662.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7920..8576)
                     /gene="uvrY"
                     /locus_tag="ACHWN2_003241"
     CDS             complement(7920..8576)
                     /gene="uvrY"
                     /locus_tag="ACHWN2_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR8932663.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8718..8789
                     /locus_tag="ACHWN2_003242"
                     /pseudo
     CDS             8718..8789
                     /locus_tag="ACHWN2_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8872..9048
                     /gene="yecU"
                     /locus_tag="ACHWN2_003243"
     CDS             8872..9048
                     /gene="yecU"
                     /locus_tag="ACHWN2_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR8932664.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            9035..9259
                     /gene="yecF"
                     /locus_tag="ACHWN2_003244"
     CDS             9035..9259
                     /gene="yecF"
                     /locus_tag="ACHWN2_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR8932665.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9292..9912)
                     /gene="sdiA"
                     /locus_tag="ACHWN2_003245"
                     /pseudo
     CDS             complement(9292..9912)
                     /gene="sdiA"
                     /locus_tag="ACHWN2_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
BASE COUNT         2510 a         2507 c         2280 g         2647 t
ORIGIN      
        1 gttattctta tttcagatca tcgtcagaat tgactccacg atcacatttc ggaccggcag
       61 aaaggaatta ttctgcaaac agtaattatg gtgttttgat ttatcttgca cctctccact
      121 tctggatata aggatattag gtatggcaac cgctggaatg cttctcaaac tcaactctca
      181 aatgaaccgc gagttttacg catccaatct ctaccttcac ctgagtaact ggtgttctga
      241 acagagtctg aacggcaccg ccactttcct tcgcgcccag gcacagagta atgtgaccca
      301 aatgatgcgc atgtttaact ttatgaagag tgtcggcgct acccccatcg ttaaagccat
      361 tgatgttcca ggtgaaaaac tgaactctct ggaagaactg ttccaaaaaa cgatggaaga
      421 atacgagcaa cgttcaagca ctctggcgca gttagccgat gaagcgaaag aactgaatga
      481 tgattcaacc gtcaatttcc tgcgcgatct ggaaaaagaa cagcagcatg atggcctgtt
      541 gctgcaaacc attcttgatg aagtgcgcag tgcgaaactt gcgggtatgt gccctgtgca
      601 gaccgaccaa catgttctga atgtcgtgtc acaccagctg cattgatcat acatcggcgc
      661 taatacattg cgccgatgaa gtttttgaga aaccgctgcc tcatctgttt gaagcagcgg
      721 tttttttaat gggattcacc ctgtggggta aacttgagtt caatcagcgc gatggctttt
      781 tggattgccc gcatggtgac cgggtctgcg gcagcgggat gatgagtaaa gtcgatattc
      841 ttcagctgac tggacatttt ttcacgaact tcaacgggcg cgattacatc gagaacatcc
      901 agaatttgtt tgataaccaa ctggcaagct accacatcag aaaccagttc ctgatcggca
      961 ttcagcggct gggacatcgt aaactcctga tagcattttg aaagccgtta tagtagcggc
     1021 ttcacatctt cagcgatagt cacatccacc gtcatcagga cacaaaaaaa cctgccggag
     1081 caggtttttt gttatcggaa catattgcct ggcggtacgt ctttgaacgt cttgcaatag
     1141 ttattgaaca tacttttcag gattttgcgc agtttcatcg cggcactccg accatttgtt
     1201 atacaggtgt tattgtcttt gcgcttataa tatgacaacc atcacaaaaa tcaatcttta
     1261 tgtgatacaa atcacataaa taccccttta atgttataaa aatgataatc aaaaaacagc
     1321 ccccctattt ctgacaccta cagatggcaa gaaatagcgc ctgccaggcg tcttttccgg
     1381 ccattgtcgc agcgctgtaa cgcgtaaaat agtgctttct cttactcttc tggctggacc
     1441 atgagacttc tgattctgac tctttcatta ataacgctgg cggggtgtac ggttactcgc
     1501 caggcccatg tgagcgaagt tgacgcggca acaggtattg tacggttggt ttatgaccag
     1561 gcttttttgc agcatgccca taccgatcgt tatgtcagtc gcgggatcgc cgatcgcgca
     1621 tgccagcagg aaggctatac ccacgcgatc ccctttggtc agccagtagg caactgtagc
     1681 ctttttgcgg gttccttatg tttgaatacg gaattcactt tgtcatatca gtgccatcat
     1741 tccgccttcc ctgtctttct ctaaatattt taatcagcga ggggatcttc gctgattaaa
     1801 gaaatagcgg taatgcgttt tattcccatt cgtattttta ataattgaag tttatatttt
     1861 accttttgca aataataaaa taacaaatta tagtgacgcc acagcaacaa atataacctt
     1921 tgtggagcac tatcatgctg aaaccagaaa tgattgaaaa acttaatgag cagatgaacc
     1981 tggaactgta ctcttcactg ctttatcagc aaatgagcgc ctggtgcagc tatcatacct
     2041 tcgaaggtgc tgccgcgttc ctgcgccgtc acgcccagga agagatgacg catatgcagc
     2101 gtctgtttga ttacctgact gataccggca atttaccgcg tattaatacc gttgaatctc
     2161 cgtttgctga atattcctca cttgatgaat tattccagga aacctataaa cacgaacaat
     2221 taatcaccca gaaaattaac gaactggctc atgctgcaat gaccaatcag gactacccaa
     2281 catttaattt cctgcagtgg tatgtttctg agcagcatga agaagagaaa ctgttcaaat
     2341 cgattattga taaattaagc ctggcaggca aaagcggcga aggtctgtat tttatcgaca
     2401 aagaactctc taccctcgac acacaaaact aatgctctcc ggcggcaggt ttctgccgcc
     2461 gtttcaaatc agtgacggca aatcttactc tgatcggtcg aaaaaccttc ttctgaaggt
     2521 ataaatttgc cttttgctgc cagaaacgct accagctctc ccgctgtcac cccttctgcc
     2581 gagcacgtgt gaaaacgtgc ctgttcacca aaacgcgctt taatcgcagc ttccagactg
     2641 gcatgcgtat attgctcgcc tgattcaatc atcatattta acacttcatg accgtgaata
     2701 gagtccatcg ttcctcctca aaaaaagcct agcgtagcga ttaccgctta tgaagacttt
     2761 gcgccagcgc aggactgaat gcttttattg tacatttata tttacacaat atgtaacgtc
     2821 ggtttgacga agcagccgtt atgccttaac ctgcgccgca gatatcactc ataaagatcg
     2881 tcaggacaga agaaagcgtg aaaaacagaa ccctgggaag tgtttttatc gtggcgggaa
     2941 ccacaattgg cgcaggcatg ctggcaatgc cgctggctgc ggccggtgtt ggttttagcg
     3001 ttacgttaat cttgttgatt gggctttggg cgttgatgtg ctacacggcg ctattactgc
     3061 tggaggtgta ccagcatgtt ccggcagata ccggtctggg cacgctggca aaacgctatc
     3121 tgggacgcta cggtcaatgg ctgacgggct tcagtatgat gttcttaatg tatgctctga
     3181 ctgcagcata catcagcggt gccggtgaat tgttggcctc cagcatcagc gactggacag
     3241 gtatttcaat gtcggcaacc gctggcgtgc tgttgttcac ttttgttgcc ggtggcgtgg
     3301 tttgtgttgg aacatcgctg gtcgatttat ttaaccgttt tctgttcagc gccaaaatta
     3361 tttttctggt ggtgatgctg gtattgctgc taccgcatat tcacaaagtg aatcttttaa
     3421 ccctgccgtt gcaacagggg ctggcactgt ctgcaatccc ggtgattttt acgtcgtttg
     3481 gttttcacgg tagcgtgccg agtattgtca gctatatgga tggcaacatt cgtaagctac
     3541 gctgggtgtt tataatcggt agtgccatcc ccctggtggc atatattttc tggcaggtgg
     3601 cgacgcttgg cagcattgat tcaacaacct ttatgggatt gctggctaat catgctggat
     3661 taaacgggct gttacaggcg ttacgcgaaa tggtggcctc tccgcatgtt gagctggcgg
     3721 tgcatttatt tgctgattta gccctcgcca cgtcatttct cggcgttgcg ttaggcttat
     3781 ttgattatct ggctgatttg tttcagcgtt caaataccgt tggtggacga ttgcaaactg
     3841 gtgcaattac ctttctgccg ccgttggcgt ttgcactgtt ttatccacga ggatttgtga
     3901 tggcgctggg ttacgccggt gtggcgctgg cggtactggc attgattatc ccttcactat
     3961 tgacctggca aagcagaaag cacaatcctc aggcgggtta ccgggtcaaa ggtggtcgtc
     4021 cggcgctggt ggtggtgttt ctctgtggta ttgctgtgat tggcgtgcaa tttttgattg
     4081 cggcagggtt gttaccagaa gtggggtgat cagatagcct caaattcctt attgggtgcc
     4141 agaattaacg ctgacaccca atgtgacctc ttaatgcagg cagcactgct taaatttctt
     4201 accactgccg cacggacaag gatcgttacg ccccggtttc tcttctgctt tgatcggttg
     4261 ctgaacagct ttttcctgcg gatgcgccat ccagtatgca tgtaaatcaa gcgccgccag
     4321 tcgaatggca tctacgctct cttcaaacgc ttctggcgac attttctcta cccgctcgaa
     4381 gttttcctca gtaccgtgca gcgcaatcgc ctccagcgct ggttttaaag aatcgggcaa
     4441 cgttgaccag tcagaaagtg ccacgccccg catatagcca aagcaccact cctcaacaat
     4501 cgtcagctcg ctgccatcaa cttctcgcaa gccgaataac ggctcaaact gctccgggaa
     4561 ttcgttcaga cgctctgcgg tatcggccat atgttgaaaa gccaggttca taaagcgcgt
     4621 catctctttc tctgacgccc aacgcggcac atagtcagcc ccaccccaca cggcaaccag
     4681 ccactgttct ggttcaatct cttgcggaga actcaacacc gccgtcaata aaccgtccag
     4741 ctccgccaca tcaaggatgg cgtggtcagt gttgtatttg gtcagaatat cgtccagcca
     4801 ttccaactca ctttcgttta acggtcccgt tttcatacgc ttttccttgt ggatctcaac
     4861 tcgccagcac ctatcttaca tgccggtccg tatcagagat actttttgag tggctttgcc
     4921 ggtgattaaa aattaaggag ggtgtaacga caagttgcag gcacaaaaaa accacccgaa
     4981 ggtggtttca cgacactgct tattactttg attttattct tatctttccc atggtacccg
     5041 gagcgggact tgaacccgca cagcgcgaac gccgagggat tttaaatccc ttgtgtctac
     5101 cgattccacc atccgggctc gggaagaaag tggaggcgcg ttccggagtc gaaccggact
     5161 agacggattt gcaatccgct acataaccgc tttgttaacg cgccaaattc ttcaggcctt
     5221 tcagccagac atccgcttga cgccgatgtc ttttaaactg gagcgggaaa cgagactcga
     5281 actcgagacc tcgaccttgg caaggtcgtg ctctaccaac tgagctattc ccgcattcat
     5341 caagcaatca gttaatcact tgattttatt atcgtctggc aatcagtgcc gccgttcgat
     5401 gcgttgcatt ctacttacct ggcgcgatga gtcaacgata tttttcacca cttttgatcg
     5461 tttgctgaaa attacgccga aacgatcact gatcaagcaa atctgcacgc gcagcgctca
     5521 aatattgcaa cattgaccac agagtcagta ccgcagccac aaagaaaagt gcaataccga
     5581 cgtactcaac ccaaatgttc ggacgccaca gcagccaggc caacgccacc atctgggcag
     5641 tggttttcac tttcccaatc caggagacgg ccacgctact gcgtttaccc aactccgcca
     5701 tccattcgcg tagcgcagaa ataataattt cacgggcgat catcgttgcc gccggtaatg
     5761 tcacccacca gctgtgataa tgctcggtta ccagcaccat ggcgatagcc acgagaactt
     5821 tatctgccac agggtcaagg aaagcaccaa accgggtact ctggttccag cggcgtgcca
     5881 gaaaaccatc gaaccagtca gtcaccgccg cgacgcagaa aatgagcgcg gcggcaaacg
     5941 gcgaccaggt gacaggcaga taaaagacca atacaaagaa tgggataagg atgacacgga
     6001 acagtgtaag caacgtaggg atattaaatt gcataatgac gggtaactat ctgttgtcag
     6061 taagattacc cctatgttgc tacagagaca tcaatgtttc aacgaccaga agatcttttc
     6121 tgccagacct tgcgaaatac ccggcacttt tgcaatttcc tcgacgctgg cgttacgtaa
     6181 accttgcaaa ccgcccatat atttcaacaa catttgccga cgttttggcc cgacgccttc
     6241 aatggtttcc agggaactgg tatttttgac cttcgcccgt tttttacggt gcccgccaat
     6301 cgcgtgatca tgtgattcat cgcgaatatg ctggataaca tgcagcgcgg gtgaatctgg
     6361 cggcaaacta aatccctcac cttccggctc aaagaacagc gtttccagtc cagccttacg
     6421 atctgctcct ttggcaacgc caagtagcag cggatgattt ttatcccatg agacatccag
     6481 ttcggcgaag acatttttcg cctgcgcaag ctggcctttg ccgccgtcga taaggatcac
     6541 atccgggatc ttactgtcgt caatggcttt accataacgc cgacgcagca cctgattcat
     6601 cgccgcataa tcatcgcccg gcgtgatgcc tgtaatgtta tagcgccgat actccgcacg
     6661 cagcgggccg ttagcatcaa acaccacaca ggaagcgacg gtttgttcgc ccatggtatg
     6721 gctgatgtca aagcactcca tccgcttcac ttccggcaat ttcaacacgc tggcaagcgc
     6781 ggtcagtcgc tggtgaacgg tagattgctg cgaaagtttg ctggttaagg ccgtcgccgc
     6841 attggtgcgc gcgagtttca gataacgcgc cctatcgccg cgaggtttgg tttgaacatt
     6901 aatcttgcgt cccgccagtt ctgaaaggga atcggcgagc agcgttttat cgctaagatt
     6961 aaaatcgagc aggatctcac ccggtaaggt gcgcatctgg ctgccttgta aatagaactg
     7021 acctacgaag gtttctacca cctcgctcag ttccgtaccg ccaggcactt tcgggaaata
     7081 gctgcggctg ccgagcactt tgccctgacg aatgaacaat acgtggacac aagccatgcc
     7141 cgcatcgaac gccacaccaa taacgtcaag gtcgtcgccg gtattggaaa caaactgttt
     7201 ttcggtgacg cgtcgcaccg cctgaatttg gtcgcgaata catgccgctt cttcaaactc
     7261 cagattctgg ctggcagttt ccatacggct aatgagttgc gtaagcacct gatcatcttt
     7321 gccagacaaa aacaggcgca catactcgac ctgctgagcg tattcttctt cactcaccag
     7381 tccttcaacg cacggtccca gacagcgccc tatctggtat tgcagacacg gacgcgagcg
     7441 attgcgataa acactatttt cgcactggcg aatggggaaa atcttttgca gtagcgccag
     7501 tgtttcacgt acggcatagc cattcgggaa cgggccgaaa tattcacctt tggcatgctt
     7561 tgcaccacga tgcatcgcca gacgcgggtg ggtatcgcca ctcaggaaga taaaaggata
     7621 agatttatca tcgcgtagca aaacgttgta acgcggctga tagagtttga tgtagttgtg
     7681 ttccagcaac agcgcttcgg tttctgtatg cgtaaccgtt acatcaattt gctggatctg
     7741 ggcgaccagc gcttcggttt tgcgcgaagc gaggttgctg cggaaatagc tggaaagccg
     7801 ttttttcagg tctttcgctt tgccgacata gataaccgta ccaccagcat cgtacatgcg
     7861 ataaacgcct ggctggctgg ttacggtttt taaaaacgct tttgcgtcaa actgatcact
     7921 cactgacttg ataatgtctc cgcattacac agaccatggc gaattgccag gtgagtcagc
     7981 tcaacatcgc catgaatgtt tagtttactg aacatacgat agcggtagct gttcaccgtt
     8041 ttcggactga gattgagctg ttctgagatc tcattgacct tctggccctt agtgatcatc
     8101 agcataatct gcaattcacg ttcagacaaa ctggcaaatg ggctttctgt tttttctggt
     8161 tcgatctggc ttaacgccat ttgttgagcg atatcagaag caatgtaacg ctgccctgaa
     8221 tagacagaac gaatcgcact cacgacttcc tgcggagccg cgcctttgct gaggtagcca
     8281 gcagcacctg cctgcatgac tttcgctggt aaagggtttt ctgtatggac cgtaagcatg
     8341 atgattttaa catcagctgt ggaacgcgcg attttacgcg tcgcctcaag accgccaatg
     8401 cccggcatac tcatgtccat tagcaccacg tcaacggcat ttgtccggca ccacttaacg
     8461 gcgtcttcac cgcacgatgc ctctccgacg acttttatac ccttaatatc ttccagaatg
     8521 cgtcgtatcc ctgcgcgcac cagttcgtgg tcatcaacaa gtagaacgtt gatcaaagga
     8581 atatctccag aaatagggat aacgctactg atagttagtc attcgtatat tagcggtttt
     8641 tattgcaact ttgaaacgtt aaaaatggtg ggtattcgat ttttctctcg tttttgtcaa
     8701 ttcgattgtc cacagttatg gtcacgcccg tcaccaacgg cttaccggat cgtttcacag
     8761 taagccctac gatgaaatgt gacaaaaatt gactttattc agcaaaaatg aaaatcagcc
     8821 cgctgaatat gtaacattaa ttaacccgaa caatacgggc aaaaaacatt catgataaaa
     8881 atatttattg gtcattatat taacgtattt tatagcactg ccgatatcac gctcaaaaaa
     8941 caaccactgc tatttttagc aaagcttatg gtatactccg ccgccttaac atttttcacc
     9001 acaaattttc attgcaacat gacgaggaaa ataaatgagt acgcctgatt tttctactgc
     9061 cgagaataat caagaactgg caaatgaagt ctcctgcctg aaagcgatgc tgacgctgat
     9121 gctgcaggcg atgggacaag ctgacgcggg ccgcgtgatg ttaaagatgg aaaaacagct
     9181 tgcgctgatc gaagacgaaa cccaggctgc agtattttcc aaaacggtta agcaaattaa
     9241 acaagcctac cgtcagtaat ataaaaccgg ctgagagcgt gcctttcagc cggttattga
     9301 caaaatcatc gctatctctg ctgatgtctt cccttccgcc gtccatttca gaatttcttt
     9361 ttcgcgcttg ctgaaattca tctctggcgt catcactatt tcatcattta aacgcatcag
     9421 agccatcaga ctttcgcgca ccagtaactg catttttaat tgcagttcat cactaagaat
     9481 gggtatttcg cgcgtgctgc aacgggaaaa ggacaaaaag cccagcgccc gatttggcag
     9541 cattaaatac tgagtgacac cgcggcgtaa accatgtgcg tgcgcggctt cccataatgg
     9601 ctgtgcttcg ctgaataagt catcattcca cattaaatgg ccctgactaa agttttcagg
     9661 gttcagcaca ggatcaattg cgagaaaatt ttttgcctga taataactaa cccacgactc
     9721 agggtaattg gtgtagaaag ccactttagg tcgcgtgaat ggtaccgggt ggcgaacaca
     9781 taacgaatag taatcgtact ccagctgctg agcctgaagc tctatttcat ggtaggcctc
     9841 ttctgcggcc tccatcctct gaaaacgcaa cagcatcgtg cgacgccagc tgaaaaaatc
     9901 cgtatcctgc atagtaaacc gcaacgcccc tgagagtgag tatc
//
LOCUS       DAZSNO010000140         9925 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZSNO010000140 DAZSNO010000000
VERSION     DAZSNO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.140"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..712
                     /locus_tag="ACHWN2_003246"
                     /pseudo
     CDS             1..712
                     /locus_tag="ACHWN2_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2318..2536
                     /locus_tag="ACHWN2_003247"
     CDS             2318..2536
                     /locus_tag="ACHWN2_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR8932666.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2754..2996
                     /gene="yiiF"
                     /locus_tag="ACHWN2_003248"
     CDS             2754..2996
                     /gene="yiiF"
                     /locus_tag="ACHWN2_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR8932667.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3326..4255)
                     /gene="fdhE"
                     /locus_tag="ACHWN2_003249"
     CDS             complement(3326..4255)
                     /gene="fdhE"
                     /locus_tag="ACHWN2_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR8932668.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4252..4887)
                     /gene="fdoI"
                     /locus_tag="ACHWN2_003250"
     CDS             complement(4252..4887)
                     /gene="fdoI"
                     /locus_tag="ACHWN2_003250"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR8932669.1"
                     /translation="MKRRDTIVRYTALERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4884..5786)
                     /gene="fdoH"
                     /locus_tag="ACHWN2_003251"
     CDS             complement(4884..5786)
                     /gene="fdoH"
                     /locus_tag="ACHWN2_003251"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR8932670.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5799..8849)
                     /gene="fdnG"
                     /locus_tag="ACHWN2_003252"
     CDS             complement(5799..8849)
                     /gene="fdnG"
                     /locus_tag="ACHWN2_003252"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8262..8264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR8932671.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9043..9876
                     /gene="fdhD"
                     /locus_tag="ACHWN2_003253"
     CDS             9043..9876
                     /gene="fdhD"
                     /locus_tag="ACHWN2_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR8932672.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2489 a         2396 c         2541 g         2499 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aacgtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaaagttgag gatatgtgtg aaagaatgaa aggtggcatc tctatgttta accacaccac
      541 aaaaacagct gataatatag agcataattt gcagctaagt tttctcgtgg gttcacgtcc
      601 gtcgatcacc aataaatact cagcaaaaga tgtaggtcca gatatacttg cattgcccgt
      661 caacgtatat catgcggatt ttaaaattaa aatcaataat gcagtggaga aggcttcatc
      721 aattgtgtta tgtgctgtgc aaaaaaatct ccatgcagca aggaaggttc ttagcgggat
      781 aataggaaat gtgccaaaaa ataataaatc aaaaacaatt atttttgatt tgcgttttct
      841 tagtgcaatt aagcagaaag tttttttggg cagtgaaaaa ccgttgtttg taaagcatac
      901 cgcaatgatg agggaggcat gtaaggaatt accgcagtca gtggaatatg ttcctttagc
      961 ctgtgaagcg catagccatc gcagcgtcgc ccttgccaaa accgtggctg cgcccatcat
     1021 ggcgacaggt gtgggcttgg cattaatgta tgcaacatca tcaggaccgt attatgcgca
     1081 aagtctttct ggttcgccgg atatcgagat gataaaaaat cgcatggctg aattgtttaa
     1141 ccagtcggat atttctgtgc gcaacaaata tagcccggct tttgataaat ggaatgaact
     1201 ggtagataaa ttaaatcacg aaagaaatac agttccgttt gcgtgtttga ccaccatttt
     1261 agcgacggcg ataaatgaaa ctcttgaggg ggatacgaat gttgccgttg tgatggggtg
     1321 taaatctgcg aaagatcgca cgatatccat tgtccttggc aactcgatgt tgcaaacact
     1381 ccttgaaaaa cgcctcgccg atagtggaga aatagaaaat ctttttgatc aacagggata
     1441 ttttagttgc gattcattaa cacccgaaga attaatgatg ttaaaagacc tttttgatat
     1501 tcgagttctt catgtctcta acaaattcaa tgttggattg caaggtaata tcaacactga
     1561 tgttttacaa gattcgtttt ttaaaaatgt cgatttcatt catgaatata actcgtatac
     1621 cgtagggatg ggagtctgac gagtaaaact cattaggatt atcagaattc ggacaatgca
     1681 ttgcaaaaat gacatacaaa gagagatgtg tgcaggtact tagccgcgag gggcatggcg
     1741 aagccataca gaaagtacca gtattcagaa ctccagtgaa agcggcatag tggccgcctg
     1801 ctcaggagtt ttgctatgac ccgtaatcgt gctgaacgtc gccaccctat gatgcgatta
     1861 aaaaaactca tcgtcgcgct gatgattgac gtcaatgcta tcggatgatg ccgggggtgt
     1921 tccggaagga attccttgtt tcccgaactg ttaccaacaa aattataacg atgagcaagt
     1981 aaagaccggt tggtggcagt actgaccggt ctgtgatgaa agattaattt caccttaaat
     2041 cctggtgctg ttaggatgct gacgtttttc ggctacacca ttcagattat gtaaataaat
     2101 atccctgaat ataatatatt catagcaccc ataactcatc ctgggcgata actaaaccat
     2161 ttttgagtaa taaatgaaac aactgcaacg ctcttattat ttctggaaaa cgcctcgcaa
     2221 aaacaaaatg acatgcaaaa atccgcattt ttttaaatgc ttttccggcg taattttcgc
     2281 acatacatta atgagtaata tatgtaaggt ggtggtaatg gcgatgaata cggtttttct
     2341 tcatttatca gaagaggcaa ttaaacggct gaacaagctt cggggctggc gtaaggtttc
     2401 acgatctgca attttacgcg aagcggttga gcaatatctg gagcggcagc aatttccggt
     2461 gcgtaaagca aagggaggca ggcaaaaggg ggaggtagtt ggtgttgatg atcagtgtaa
     2521 ggagcataag gaatgaaaaa aatttttcat tcttgaatat aaaaaacaga tgcccttatt
     2581 ctggtattaa tacaaggctg ttttacttga acttataata actgcaactg ttacatcgta
     2641 tctggaaaac gcctcgcaaa aataaaaaat gatgcgtaaa tgagctttct ttaaagattc
     2701 ggcaagggta cttttctctc catagattca aaaatgatat tggcgagata tttatgaact
     2761 ctctggcagg tatagatatg ggcagaattt tactcgattt atcgaatgag gtgattaagc
     2821 aacttgatga tctcgaggtg cagcgtaatc ttcctcgtgc ggacctatta agggaagcgg
     2881 tagatcaata cctgataaat caatcgcaaa cagcaagaac cagtgttcct ggcatctggc
     2941 aagggtgtga ggaagatggt gtcgaatatc agcgtaagct gcgcgaggaa tggtaatggg
     3001 aaaaaatgat gtcaatcaga ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt
     3061 gaacgcctta tccggcctac aaagtcatgc aaattcaata tattgaagtt tattggtagg
     3121 cctgataagc gcagcgcatc aggcaatcat acgcttgtca tcagtgacat acccataacc
     3181 ttatcgatgt gttggaggga agaacgaggc aaacagacaa ttgcggcatg atcttgaatg
     3241 ggtaacgcgg aatacgagag atggtgtcgt cacaccctat gagctgtaat ccgggcgacc
     3301 tgcgccaccc ggaaaacgtc agagattact ctccttcacc cggaaacagg aacgggttaa
     3361 tggaactacg ggcgtagcct tcttgctcca ttcgcgcgtc cagtaccaga gaggcgaggt
     3421 catctgccac ggcttcaact ttcggttctt tttcctgata aagaatcttc aggtaagtgc
     3481 cgcagtcatc gcagctttcg gctttaatcg cggcctgttc gtcatccagc gaccagtaat
     3541 gcagtttgcc gctctgctcg cagttgctgc attttacgcg cactacgtgc cattcggttt
     3601 cacacaggtt gcagtgcagg taacgcagac cctgagtggt gccaatttgc accatgctgg
     3661 acaccggcat agagccacat accgggcaat attgacgttg ttcgccgtat tcagcgcggg
     3721 ctttgccggg gatcagattg gccatctgcg cccagtagag cgacagcgca gcccagataa
     3781 acggcgcttt atcgctgctg acggacgaga aatcagaggc aaacagcgcg ctggccatat
     3841 cttccagctc ctgagtcgat gccttctcca gattctcaat cactgccagc gccgggccgc
     3901 tcatttcagg tttcagctca gcaattagcg ccatcagcag cttttgccag tgcttatcac
     3961 gcggcagaac gtgaatatcc agcgggggct tgccctgtgc gctggcttct ttaatgcgcg
     4021 cggtcagatc catctccagc ggatggtcgt acagcaccac tttctgggcg tgggcgataa
     4081 gcgcagcaaa gcgcaggtaa tcacccagcg gattattttc tgccagctcg cgcagacgct
     4141 cggcgcggcg gttgtataaa ttcttgagcc gagggaacaa taacggcgga atcatatccg
     4201 ccgtacgttt ctcgctcgaa cccagctcat cttgcgggat tatgcgaata ctcattcagc
     4261 tttcttttcc gttgtcttgc ggacctcacg gtaccagcgc gggtgatgtt tcttcgccca
     4321 ggcgctggta acccatcctt ccaccatggc ggtaatcgtg cctttcaccc aaagggcggc
     4381 gtagatatgc accatgataa ccacaattaa cgccactgcg gcaaatgaat gcagcattaa
     4441 cgcgaatcgg atcaccggga ttgagaaagc aggcgcaaaa taaggacgcc agataatcac
     4501 gccgctcacc agcagcagaa ccaggaaaat aatcgccgcc cagaaaacgc atttctgacc
     4561 gaagttataa cgcccggtgt cacctacttc ctcgttgacg acgatcttac gaatattctt
     4621 cgcccaaaag atatcatccc gattgattag gttttgatgc cagtaacgga aaaacatgat
     4681 gatgaacgag gcaaacataa ccacgccgac aaacgggtgc agaattcgcg ccagctgcgg
     4741 tgtgcccatg atttgcatca accagttgaa ggacgggaac aaaaagccca gcccgctcac
     4801 cgccgccagg atgaagcaga aggcggtgat ccagtggttg atacgttcca gcgcggtgta
     4861 gcgcacgatg gtgtcacgtc gtttcatttg cgctcctcgt ctttctcttc gtgcagatta
     4921 ttctcttcct catccgcacg gttcggaccg acacccacgt agtggaagat actggccgca
     4981 aaggtagccg caaagccaac agccgcgagc ggtttccaga tgcctttcca gaatttcacg
     5041 gtttcgctga tttccgggtt ctccggcaag ccatgataca gatttggctt gtcagcatgg
     5101 tgcagcacgt acatgacgtg tgtaccaccg acgcctgccg gatcgtacag acccgcattg
     5161 tcgtaaccgc gggttttcag ctcagcaacg cgctcgctcg ccagcgtttt catcgactct
     5221 ttcgtgccga agtgaatcgc gcccgttggg caggtcttca cgcaggctgg ttcttgccca
     5281 accaccacgc ggtcaacgca cagcgtacat ttgtagacgc ggttgtcttc cgggttgagg
     5341 cgcggaatgt cgaacggaca gcccgcaatg caataaccgc agccaatgca ctgctcggac
     5401 tggaagtcga cgataccgtt ggcatactga ataattgcac cttccgccgg gcacgctttc
     5461 aggcagcctg gatcggaaca gtgcatacag ccgtctttgc ggatcagcca ttccagtttg
     5521 tcgttctgct ccacttccga gaagcgcatt accgtccacg atttggcgct taaatcattg
     5581 gggttgtcgt acaccccaat gttattgccg acggtatcgc gaatgtcgtt ccactctgaa
     5641 cacgccacct gacaggcttt acagccgata caggtggtaa cgtcgatgag tttcgccact
     5701 tcttcctgga agtcccgcgc ctgaggcgcg ggggtcagac cgttagtcgc ggaacgacga
     5761 atgatatctt gagattgata agccatatgt cgtctccgtt acaccttttc cacattcaca
     5821 aggaaggact taaactccgg cgtctgcgtg ttcgcatcac caacgaatgg cgttaaagta
     5881 ttggcaataa agcctttttt agctacgcct tcatagcccc agtgaatcgg aataccgatg
     5941 gtatcgatat ctttgccgtt cgctttcagc gtgcgaatac gtttggtcac caccgctttg
     6001 gctttgatat agccacggtt ggaggagact ttcacggtat cgccctgggc aatgccaagt
     6061 ttattcgcca gcgactcccc gatttccaca aactgctctg gctgcaaaat cgcgttcaac
     6121 agcgcgtgtt tggtccagta gtggaagtgc tcggtcagac gataggtggt tccgacatac
     6181 gggaacttat cggctttacc caatgcttcg gcgtcgtctt taaagatacg tgcagctggg
     6241 ttcgagataa cgtttggatg cagcgggtta gttcccagcg gcgtttcaaa cggctcgtag
     6301 tgttccggga acggaccttc cgccatctta tcgagggcaa acagacgccc catgccttcc
     6361 tgctgcatga taaacggccc gacgccgcta cccggaggcg ctgcgctgta gtccggaata
     6421 tcccagccgg tccacttagt gccgtcccat ttcagcaact gacgcttcgg atcccacggg
     6481 ttaccctgcg gatctgcgga ggcgcggtta tacagaatgc ggcggttaag cggccatgcc
     6541 catgcccagc ccagcgtgtt accgaggcca gacggatcgg cgttatcacg acgcgccatc
     6601 tggttgcctt ccggcgtcca gctaccggcg aaaatccagc agccacagga ggttgtaccg
     6661 tcatcgcgca attgggcgaa cgagctaagt tgttggcctt tcttgacgat aaccgcaccg
     6721 gttgccggat cggtaatatc ggccagcgcc ttaccgttgc tctccatcgc tacttcttcc
     6781 gacttcggct catgcggaat ggcgtagttc caggtcatgt tcagcacctg atccgggttc
     6841 gcgccaccct gttcggcata catcttgcgc aagcgcagga agataccgga gaggatctca
     6901 ccgtcagtca gcgcaatccc cggggcgtcc gcacctttcc agtgccactg caaccagcga
     6961 cctgagttaa cgattgaacc gttctcttct gcgaagcagg tcgatggcag acggaacact
     7021 tcggtctgga tcttcgacga gtcaacttcg ttcagctcac cgtggttctg ccagaagtta
     7081 gaggtttcag tgttcagtgg gtcgatggtg acgaggaact tcagtttcga cagacaaccg
     7141 atcactttgt ttttgttcgg gaatgaggca acagggttaa agccctggca gatatagcca
     7201 ttgaccttgc cctctttcat catctcgaag tattgcagga cgtcgtagcc tttatcccac
     7261 ttcggcaacc agtcaaagcc ccagctattt tccgccgtcg ctttatcacc aaagaaggcc
     7321 ttcatcatag agacgaagaa tttcgggtag ttgccccagt agtttacctg gccttccagc
     7381 agcggttttg gcgtgttggc ggtaaggtag gtttgcagat cggtctgctt ctcgcttggc
     7441 agcgtcatgt aacctggcag actctgcgac agtagcccca ggtccgtcag cccctgaata
     7501 ttggagtgac cgcgcagggc gttaacgccg ccgccggcca tccccatatt gccgagcagc
     7561 agctggatca tcgccatcgt acgaatgttt tgcgcaccaa cggagtgttg cgtccagccg
     7621 agggcataca ggaacgaggc agttttatcg tgagcactgg tttctgcgat gtattcgcag
     7681 actttcagga acgcgtcttt tggcgtacca cagatgtttt caaccacatc tggcgtgtag
     7741 cgggaaacgt gctgtttcag caagttccac acgcagcgcg gatgttgcag cgtggtatcg
     7801 cgtttggcga agccgttttc gtccagttca taagtccagg aggatttatc gtacttgcgt
     7861 ttttccgcgt cgtagccggt gaacaggcca tcttcaaagc cgtaatcctc acgcacgatc
     7921 aggctggcgt tggtataggc ttcggtgtat tcgtggttga atttttcatt gttcagcagg
     7981 tacagcaata cgcctgacag gaaagcaatg tcagtaccgg aacgaatagg ggcatagtag
     8041 tcagctaccg ccgccgtacg cgtaaagcga ggatcgatca caatcagctt cgcgccgttg
     8101 tgaattttgg cttccatcgc ccagcggaac ccgaccgggt gagcttcagc ggcgttaccg
     8161 cccatcacca cgacgaggtt ggcgttcttg atgtcgaccc agtggttggt catcgcaccg
     8221 cgaccaaatg ttggagcaag acttgctacc gttggtccgt gtcagacacg cgcctggttg
     8281 tcgaccgcga gcatacccag cgcgcgggag aatttttgcg ttaaatagcc ggtttcgtta
     8341 ctcgacgcgg aagcacacag catcccggtg gagagccagc ggttaacagt cacgccttcg
     8401 gcgttttgcg caatgtagtt agcatcgcgg tcttctttca tcagtttggc gatgcgatca
     8461 aacgcctctt cccaactgat ttgctgccat ttatcagaac ctggcgcacg gtattccgga
     8521 aacttcagac ggctttcgga gtggatgaaa tccaccaggc cagcgccttt cggacaaagt
     8581 gcaccgcggt tgaccgggtg atccggatcg ccttcgatat ggaagataga tgctttggcg
     8641 ttttttgctc cgtcaccgag gctgtacatc aacagcccac agcctacgga acaataggtg
     8701 caggtattac gggtttcgcg ggtgcgcagc agtttatact gccgggtttc cgcgagtgct
     8761 acgctgggtg caaaacccag tgccgctgcc gtggtgcctg ccataccgcc agcgcagatc
     8821 ttaaagaact gccttctgct gacctgcatg gattgctcct tgtttcgaca ttgtcacgtc
     8881 ccatttacat tcgcttgctg cgtgtgcagg gagtgggagt tatttttctt tgcggaaggg
     8941 gccgcaaagg tccagaattg gctcaatttc cctccatcta ggaagggttt gtaacagaat
     9001 accataatgt tggtgtgtat gttcttatct ggttaagaga aagtgaaaaa aacacagcga
     9061 aaagaaatcg aaaatgtgac aaatatcaca ggtgttcgtc aaattgagtt atggcggcgt
     9121 gatgatttac aacacccacg gttagatgag gtcgcggaag aagttcccgt tgcgctggtc
     9181 tacaacggca tttcgcatgt ggtgatgatg gcgtcgccca aagaccttga gtactttgcg
     9241 ctcggttttt cgctttccga ggggataata gaaagtccgc gcgatatctt cggcatggat
     9301 gtcgttcctt cctgtaatgg tcttgaagta caaattgagc tttccagccg ccgctttatg
     9361 gggttgaagg agcgccgccg ggcgctggcg ggacgtacgg gctgtggcgt atgcggcgtg
     9421 gagcaactta atgacatcgg aaaaccggtg cagccgctac cgttcaccca gacgtttgat
     9481 ctcaacaaac tggatgatgc attacgtcat ctcaacgatt ttcagccagt ggggcgactg
     9541 actggttgta ctcacgccgc tgcctggatg ttgccatctg gcgaactggt cggcgggcat
     9601 gaagacgtgg gtcgccatgt ggcgctggac aaactgttag gccgccggtc acaagaaggg
     9661 gaaagctggc agcaaggtgc ggtactggtt tccagtcgtg ccagttatga aatggtgcaa
     9721 aagtcggcga tgtgcggcgt agagattttg tttgcggtgt ctgccgcgac cacgcttgct
     9781 gtagaagtgg ctgagcgctg taatctgaca ctggtaggtt tttgtaaacc gggtcgggca
     9841 acggtttata cccatccgca gcgtttgagc aattaattaa aataagtcct gcgatatata
     9901 ttaaattgca ggacttattc atttc
//
LOCUS       DAZSNO010000141         9917 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZSNO010000141 DAZSNO010000000
VERSION     DAZSNO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.141"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            326..1321
                     /gene="pgl"
                     /locus_tag="ACHWN2_003254"
     CDS             326..1321
                     /gene="pgl"
                     /locus_tag="ACHWN2_003254"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR8932673.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1362..2315)
                     /locus_tag="ACHWN2_003255"
                     /pseudo
     CDS             complement(1362..2315)
                     /locus_tag="ACHWN2_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2499..3551
                     /locus_tag="ACHWN2_003256"
     CDS             2499..3551
                     /locus_tag="ACHWN2_003256"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFR8932674.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3627..5060
                     /locus_tag="ACHWN2_003257"
     CDS             3627..5060
                     /locus_tag="ACHWN2_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFR8932675.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5243..7423
                     /locus_tag="ACHWN2_003258"
     CDS             5243..7423
                     /locus_tag="ACHWN2_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR8932676.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7564..8847)
                     /locus_tag="ACHWN2_003259"
     CDS             complement(7564..8847)
                     /locus_tag="ACHWN2_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR8932677.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(8982..>9917)
                     /locus_tag="ACHWN2_003260"
     CDS             complement(8982..>9917)
                     /locus_tag="ACHWN2_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8932678.1"
                     /translation="TEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILAS
                     RGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTL
                     SDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMEL
                     AVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCK
                     EILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQ
                     ISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2399 a         2318 c         2519 g         2681 t
ORIGIN      
        1 atttgtgccg gaagtgagtt gattgttgcg cgaatgggaa gttggccaga agttgttatg
       61 gcatagtgtt tacggtcgtt gatacaggta aacaaaagcg gggagagtca tactccctga
      121 atagtcgcga aaatatctgt ggcattgtca aattcgtgtc ggtgactaat attttagtaa
      181 tatgccattc gaaatggagt gctaacttat tgatttaatt gatgtgcgat attgtatttt
      241 atcgtgtttt gttgtctgtt ttttatccag taagtgttgt tatatgtatg aaagataagg
      301 gatttatcat ttttgaggtg tgttcatgaa gcaaacagtt tatatcgcca gccctgagag
      361 ccagcaaatt cacgtctgga acctgaatca tgaaggcgca ctgacgctga cacaggttgt
      421 cgatgtgccg gggcaggtgc agccgatggt ggtcagcccg gacaaacgtt atctctatgt
      481 tggtgttcgc cctgagtttc gcgtcctggc gtatcgtatt gccccggacg atggcgcact
      541 gacctttgcc gcagagtctg tgctgccggg tagtccgacg catatttcca ccgatcacca
      601 ggggcagttt gtctttgtag gttcttacaa tgcgggtaac gtgagcgtaa cgcgtctgga
      661 agatggcctg ccagtgggcg tcgtcgatgt agtcgagggg ctggacggtt gccattccgc
      721 caatatctca ccggacaacc gtacgctgtg ggttccggca ttaaagcagg atcgcatttg
      781 cctgtttacg gtcagcgatg atggtcatct ggtggcgcag gaccctgcgg aagtgactac
      841 cgttgaaggg gccggcccgc gtcatatggt attccatcca aacgaacaat atgcgtattg
      901 cgtcaatgag ttaaacagct cagtggatgt ctgggaactg aaagatccgc acggcaatat
      961 cgaatgtgtc cagacgctgg atatgatgcc ggaaaacttc tccgacaccc gttgggcggc
     1021 tgatattcat atcaccccgg atggtcgcca tttatacgcc tgcgaccgta ccgccagtct
     1081 gattaccgtt ttcagcgttt cggaagatgg cagcgtgttg agtaaagaag gcttccagcc
     1141 aacggaaacc cagccgcgcg gcttcaatgt tgattacagc ggcaagtatc tgattgccgc
     1201 tgggcaaaaa tctcaccaca tctcggtata cgaaattgtt ggcgagcagg ggctactgca
     1261 tgaaaaaggc cgctatgcgg tcgggcaggg accaatgtgg gtggtggtta acgcacacta
     1321 accgctgatt taaccggcgc agtctctcct gcgccggtgt attaacttat ctcctgtaac
     1381 gcatgtctct ggcgttcgac gatattggtc cacaaattgt ctttatcgtc agtccacaaa
     1441 ttaatcagca aggaaaaaaa gcgttcagct gccggagaaa gcacggcatc tttgcggcga
     1501 ataatcccca atgtccgacg tataacgggt tccaccagtg ggataccaat aatcgaagaa
     1561 tagggcgcat ggggcattgc cagaccagga agcgccgaaa tccccagtcc tgcctccact
     1621 agtcctaatg acgtcgaaag atgacgcact tcgtaaaacc agtccagctt ccagggcttg
     1681 tcggctagct gttgttctat caacaatcgg ttgccgctgg aggaacgtac gccaatcatt
     1741 ttgtaaccca ctaactcctg ccattctaca agctactttt tggccaatgg atggtcacgc
     1801 cgacaggcca gcacgaatgg ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg
     1861 acgttgttca tgtttatgcc aaagtctgat tcgttacaca gcaccgattc catgcaatta
     1921 ttcgtgcctt gttccagaat ccgcacttta atattgggat acagctcatt aaatttaccg
     1981 attgccagcg gtaaaaaata aaatactgcg gtcggaatac acgctaatgt caccatacca
     2041 cgatgataag cattcatatc acgaatatta aaaagcgttt catcgaattt ctttattaat
     2101 tcccgcgcct ccgggagcaa cctttttcct gcttttgtta acgtaacctt gcgagttgta
     2161 cgctcaaata gttgaatatg taaatcttcc tccatctttt ttattcttcg cgttaatgca
     2221 ggttgcgtaa tattgagtaa tttagcggca ttgttaaatg aactggactc tgccagtatg
     2281 acaaatgcct tcatacttga taattcatgc ttcattatta ctccggaaaa tggaagcgac
     2341 gattttgggg ggctggtcgt taaaaatttt aactgcattt agccaactta aattaatgaa
     2401 aaaatgttat taatcgttga gctaaagtca ttagagatgc tttgtcctta atgtaaccat
     2461 atcgcaataa gttatgtttt taaattgagg gcattattat gaaaaaaata ccctgcgtga
     2521 tgatgcgagg tggaacctcg aggggcgcgt tcctgttagc ggaacattta cccgaagatc
     2581 aaacgcagcg cgataaaata ttgatggcaa ttatgggttc cggtaacgat ctggaaattg
     2641 acggtattgg cggcggtaat ccactgacca gtaaagtcgc cattattagc cgttccagcg
     2701 atccgcgtgc tgatgtcgat tatctgtttg ctcaagttat cgtccatgag caacgtgtcg
     2761 ataccacgcc taactgcggc aatatgctgt ctggcgtagg ggcattcgcc attgaaaatg
     2821 gtttgattgc agcgacttcg ccagttaccc gtgtacgtat ccgcaacgtc aatacgggta
     2881 cgttcatcga agctgatgtg caaacgccaa atggtgttgt cgagtacgag ggtagcgcca
     2941 gaattgacgg cgtaccgggt actgccgcac cggttgcgct cactttcctg aatgccgctg
     3001 gaacgaaaac tggaaaagtt ttcccgactg ataatcagat tgattatttt gacgatgtcc
     3061 cggtgacctg tatcgatatg gcgatgccag tcgtcattat tccggctgaa tatctgggaa
     3121 aaacaggtta tgaattaccg gcggaactgg atgccgacaa agcattatta gcccgcattg
     3181 aatctatccg gctacaagcg ggtaaagcaa tgggcttagg tgatgtcagt aatatggtta
     3241 tccctaaacc tgtgcttatt tctccagcgc agaaaggcgg ggcaattaat gtgcgttatt
     3301 ttatgccgca ttcttgccat cgcgcgctgg cgataaccgg tgctattgct atttccagta
     3361 gttgtgcatt ggaaggcacc gtcacccgac aaatcgtccc ttctgtagga tatggcaata
     3421 tcaatattga acaccccagt ggtgcgctcg acgttcattt aagtaatgaa ggtcaggatg
     3481 ccacgacgtt acgcgcatct gttattcgga caaccagaaa aatattttcc ggtgaagttt
     3541 atcttccctg aaaaaattcg ttgtcaggat aaggacaatc aataaaggac ttctgtatga
     3601 gtcatacaga aagaacagga ttttaaatga ataagaaatc gttatggaag ctaattctga
     3661 tattagcgat cccatgtatt attggtttta tgccagctcc ggcaggatta agcgaactgg
     3721 cgtgggtgct ttttggtatt tacctggcgg ccattgtggg tctggttatc aagcctttcc
     3781 cggaacctgt cgtactgtta attgccgttg ctgcctcaat ggtggtggtc ggtaacttat
     3841 ccgacggtgc gtttaagacc accgccgtat taagcggtta ctcttcaggt actacctggc
     3901 tggtgttctc ggcgtttacc ttaagcgccg catttgtgac caccggttta ggtaaactta
     3961 ttgcctatct gctgattggt aaaatcggta acaccatgct gggtctgggt tacgttacgg
     4021 tattcctcga tctggtactg gctccggcaa caccgtctaa caccgcgcgt gcgggcggca
     4081 ttgtgttacc gatcatcaac agcgtggcgg tggctttggg gtccgaaccg gaaaaaagtc
     4141 cgcgtcgtgt cggacattac ctgatgatgt ccatttacat ggtcaccaaa accaccagct
     4201 atatgttctt taccgcaatg gcggggaaca ttctggcgct gaaaatgatc aacgacattc
     4261 tgcacctgca aattagctgg ggtggatggg cgctggccgc cggattgccg ggcatcatta
     4321 tgctgctggt caccccgctg gtaatttaca ccatgtatcc accagaaatt aaaaaggtgg
     4381 ataacaaaac catcgctaaa gcgggccttg ccgaactggg accgatgaaa atccgcgaaa
     4441 aaatgctgct cggtgtcttt gtgctggcgc tactgggctg gattttcagt aagtctctgg
     4501 gggttgatga atccactgtg gcaatcgttg ttatggcgac catgctgctg ctgggtatcg
     4561 ttacctggga agacgtggtt aaaaataaag gcggctggaa taccttaatc tggtacggcg
     4621 gtattatcgg cttaagctcc ttattatcga aagttaaatt tttcgaatgg ttagctgaag
     4681 tctttaaaaa taacctggca tttgatggtc acggtaacgt tgctttcttc gttattattt
     4741 tcctcagcat catcgtgcgt tatttcttcg cttccggtag tgcctatatc gttgctatgt
     4801 tacctgtatt tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta accgcgctgg
     4861 cactgttgtt ctccaactcc tatggcggca tggttactca ctatggcggc gcggcaggtc
     4921 cggtcatctt tggcgtgggt tacaacgata ttaaatcctg gtggttggtc ggtgcggtac
     4981 tgacgatatt aaccttcttg gtgcatatca ccctcggcgt gtggtggtgg aatatgctga
     5041 tcggctggaa catgctgtaa atatactcgt catacttcaa gttgcatgtg ctgcgtctgc
     5101 gttcgctcac cccagtcact tacttatgta agctcctggg gattcactca cttgtcgcct
     5161 tcctgcaact cgaattattt agagtatatc catttattat ctttctgcgc acttcacggt
     5221 gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt ttctcgccag
     5281 taataacgaa ataattgccg aagaacattt caccggcgaa attaaaaaag aagaagcaaa
     5341 aaaaggcact attgcctggt ctattctctc ttctcataat acgtccggaa atatggataa
     5401 acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg gtattgtaca
     5461 gaccgctaaa gcgtccggaa tggaacgttt cccgctgccg tatgtgctga ccaactgcca
     5521 taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt ttggtttatc
     5581 ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca tccatcaata
     5641 tatgcgtgag atgatggctg gcgggggcaa aatgatcctc gggtcagaca gccacacccg
     5701 ttacggtgca ttagggacaa tggcagtcgg tgagggtggc ggtgagttgg taaaacagct
     5761 gcttaatgac acctgggata tcgactatcc gggcgtggtt gcggtgcatc tgaccggaaa
     5821 acctgcgccg tatgtggggc cacaggatgt ggcgctggct atcattggcg cggtgttcaa
     5881 aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta gcgcgctctc
     5941 taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt taagctccgt
     6001 ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg gtcaggatta
     6061 ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg ttgatttaag
     6121 cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac
     6181 actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg aacgcgtggc
     6241 gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc tgaaagtgca
     6301 gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg cggcggcgaa
     6361 tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcggttt acccgtcatc
     6421 acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga ttggcgcagg
     6481 cgcaatcatc agaaccgcat tctgtggccc atgctttggc gcgggcgata cgccaatcaa
     6541 caacggtttg agtattcgcc acaccacgcg taacttcccg aaccgcgaag gctctaagcc
     6601 agccaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg ctgcgactgc
     6661 ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca acgtgccgga
     6721 gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg tgaaaggggc
     6781 aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat tgggtgcgct
     6841 gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtca ccaccaccga
     6901 cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg gtctggcggg
     6961 gaatgtcagc gagctgacag aggtgtttgc gcgcattaag cagattgctg gtcaggagca
     7021 tattgatccg ctgcaaactg aaattggcag catggtctat gcggtgaaac caggcgatgg
     7081 ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt ggcggtctgg cgaatattgc
     7141 cgaggagtac gcgactaaac gctaccgttc taacgtcatc aactggggga tgttaccgct
     7201 gcagatggcg gaagtgccaa cctttgaagt gggggattac atttacatcc ctggcattaa
     7261 agcggcgctg gataatccgg gtacgacgtt taaaggttat gtgatccatg aagatgcgcc
     7321 ggtaacggaa attacgcttt acatggaaag cctgacggct gaagagcgcg agattatcaa
     7381 ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg taaaaagcgc catgtgaatg
     7441 taggtcgcat tcggcactta ttgtcggatg cgatgctggc gcatcttatc cgacctacaa
     7501 atcgcatcga accgtaggcc ggataaggcg tttacgccgc atccggcaaa tagttaatag
     7561 ctcttacttc ttctcctctg caaccacttt gctgcccacg ccgcggttat tgtattccca
     7621 catgcggttg tagttagtgt cattcagatt gcgctgtatt tcgtcgttat catctacgct
     7681 gccggtatta cccgcaaacg gacgattaga gatcaccgca tcggcccacg gtttagccgt
     7741 gttaaaacct tcgttgatgg cgctatcacg gatcaccacc tgaccgttgg tattagcatc
     7801 aacatccagc gagcggccca gttgcgccac gccatcaccg gaagcattga aacggctgtt
     7861 tacggcgagg aaaccgtaat agatgttaga cagcgtagcc ggtgcaaaca catatgcttc
     7921 ttgctgggta cgtgagttca ccacgcggaa ttcggtgtta tcgaacacca ctgcgccgcg
     7981 accagaaacg atatccacat ccccttcaat atagctgttg gtcaccagcg tacgcggctg
     8041 acggttggtt tccagacggt tctgcacacc gctgttggtg acaaagaagg tgttctgacg
     8101 accgagaatg ttaacgttgt taatctgtac ttggtcaccg tcagtacgca gtgctaccgc
     8161 cggatggtta cctgcatcta cgctatcgcc cagcgtgttt tcgatggtca gattttgcag
     8221 ttgcagtcca ttgttttgtg accagaagac cgcagaacac agaacaccga tactgtcgct
     8281 gcgtttgctc tggcagctat cgtacatata ccacgctggt ttacctggca tatatttgcc
     8341 gcgcgggttg acgtcgtgac gccagtcggc agggctcatg cccccatcaa gggaaagccc
     8401 aatcttcaca tcaatcggtt tttcacctgt accgtacaga gtgattccac ctggagcagc
     8461 agggacatac accgttccct gatactcacc aggcatcacg gcaatatact ggcgcttgtt
     8521 ggtgcgcttg ataattgccg catctaccgc cgcctgaatc gtggtatgcg ttacaccttg
     8581 agtacccgcc gggccgacaa caaagtcagg ttgcgcaggc agggttatcg gggacggatt
     8641 ccacgctgca gcacctggtg tcagggatgc aaaatagtgt tgcgcatcga aattcagcgc
     8701 ttcttttgcc gacagaatcg ggcgagaaga ggtaccaggc gcagtttgat cagaaggacg
     8761 ttgatcgggt ggtgttgagc tacaggcggt cagcgtcacg ccaaaagcca atgccagcgc
     8821 cagacgggaa actgaaaatg tgttcacagg ttgctccggg ctatgaaata gaaaaatgaa
     8881 tccgttgaag cctgcttttt tatactaagt tggcattata aaaaagcatt gcttatcaat
     8941 ttgttgcaac gaacaggtca ttatcagtca aaataaaatc attatttgat ttcaattttg
     9001 tcccactccc tgcctctgtc atcacgatac tgtgatgcca tggtgtccga cttgtgcccg
     9061 agaagatgtt gagcaaactt atcgcttatc tgcttctcat agagtcttgc agacaaactg
     9121 cgcaactcgt gaaaggtagg tggatcccct tcgaaggaaa gacctgatgc ttttcgtgcg
     9181 cgcataaaat accttgatac tgtgccggat gaaagcggtt cacgacgagt agatgcaatt
     9241 atggtttctc cgccaagaat ctctttgcat ttatcaagtg tttccttcat tgatatcccg
     9301 agagcatcaa catgcaatgt tgtagggatg gcaattttta cgcctgtttt gctttgctcg
     9361 acataaagat atccatctac gatatcagac cacttcattt cgcataaatc accaactcgt
     9421 tgcccggtaa caacagccag ttccattgca agtctgagcc aacatggtga tgattctgct
     9481 gcttgataaa ttttcaggta ttcgtcagcc gtaagtcttg atctccttac ctctgatttt
     9541 gctgcgcgag tggcagcgac cgggtttgtt gttatatggc cttcagctat tgcctctcgg
     9601 aatgcatcgc tcagtgttga tctgattaac ttggctgatg ccgccttgcc ctcgtctatg
     9661 tatccattga gcattgccgc aatttctttt gtggtgatgt cttcaagtgg agcatcaggc
     9721 agccccctcc ttattgcttt aattttgctc atgtaattta tgagtgtctt ctgcttgatt
     9781 cctctgctgg cgaggatttt ttcgtagcga tcaagccatg aatgtaacgt aacagaatta
     9841 tcactgttga ttctcgctgt cagaggcttg tgtttgtgtc ctgaaaataa ctcaatgttg
     9901 gcctgtatag cttcagt
//
LOCUS       DAZSNO010000142         9826 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZSNO010000142 DAZSNO010000000
VERSION     DAZSNO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.142"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            383..1501
                     /locus_tag="ACHWN2_003261"
     CDS             383..1501
                     /locus_tag="ACHWN2_003261"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR8932679.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FMALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="ACHWN2_003262"
     CDS             1567..1815
                     /locus_tag="ACHWN2_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFR8932680.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="ACHWN2_003263"
     CDS             1880..2248
                     /locus_tag="ACHWN2_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR8932681.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHWN2_003264"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHWN2_003264"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR8932682.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHWN2_003265"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHWN2_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR8932683.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHWN2_003266"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHWN2_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR8932684.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHWN2_003267"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHWN2_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR8932685.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVEVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHWN2_003268"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHWN2_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2407 a         2722 c         2379 g         2318 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttatggc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactacct ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg caggtagact ggccccctga
     9781 atctccagac aaccagtatc acttatttaa gtgatagtct taatac
//
LOCUS       DAZSNO010000143         9822 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZSNO010000143 DAZSNO010000000
VERSION     DAZSNO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.143"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(75..419)
                     /locus_tag="ACHWN2_003269"
     CDS             complement(75..419)
                     /locus_tag="ACHWN2_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR8932686.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            551..2461
                     /locus_tag="ACHWN2_003270"
     CDS             551..2461
                     /locus_tag="ACHWN2_003270"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR8932687.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQIVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2519..3214
                     /gene="tsaB"
                     /locus_tag="ACHWN2_003271"
     CDS             2519..3214
                     /gene="tsaB"
                     /locus_tag="ACHWN2_003271"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR8932688.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3254..3835
                     /gene="yeaY"
                     /locus_tag="ACHWN2_003272"
     CDS             3254..3835
                     /gene="yeaY"
                     /locus_tag="ACHWN2_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR8932689.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4041..5726
                     /gene="fadD"
                     /locus_tag="ACHWN2_003273"
     CDS             4041..5726
                     /gene="fadD"
                     /locus_tag="ACHWN2_003273"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR8932690.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            5808..6923
                     /gene="rnd"
                     /locus_tag="ACHWN2_003274"
     CDS             5808..6923
                     /gene="rnd"
                     /locus_tag="ACHWN2_003274"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR8932691.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(6977..7942)
                     /gene="yeaX"
                     /locus_tag="ACHWN2_003275"
     CDS             complement(6977..7942)
                     /gene="yeaX"
                     /locus_tag="ACHWN2_003275"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR8932692.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(7998..8615)
                     /gene="yeaW"
                     /locus_tag="ACHWN2_003276"
                     /pseudo
     CDS             complement(7998..8615)
                     /gene="yeaW"
                     /locus_tag="ACHWN2_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(8957..9565)
                     /locus_tag="ACHWN2_003277"
     CDS             complement(8957..9565)
                     /locus_tag="ACHWN2_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8932693.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            9735..>9822
                     /locus_tag="ACHWN2_003278"
     CDS             9735..>9822
                     /locus_tag="ACHWN2_003278"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8932694.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         2376 a         2412 c         2586 g         2448 t
ORIGIN      
        1 ctctggtcga tttatgtaaa accgaccagt attatcgata aattatcgtc tgacttattt
       61 tgcttcgata aagcttacac cgcagccaca atcttaattt caactttata cttcgggttc
      121 atcaaacccg cttgtaccgt gcagcgcacc ggcgcatgac ccgcgacaac ccaggcatcc
      181 cacgctttat tcatcgccgc gaagtcgttt ttatcggcca ggaaaatggt ggcatccaga
      241 atgctcgatt tattgctgcc ctgtttttcc agcacggcgt caatctgtgc cagcgtgttg
      301 gcggtttgct caaaggcatc ggcgtcgagg ttttccggta caccagtgta gtagagcgtg
      361 ttgttgtgga ttactacatc agaccagcgg gcttcagcat cgatacgaac gatagtcatc
      421 attttgtcct cattatactt ccattttcgt gctgcaaaga ctgccacaat gtgaggttgc
      481 cgtcaccact tcaactggcg aaagcgccct catcctgaca taatccctgt tcaaatcaac
      541 agggggtagt gtgacggacg attttgcacc agacggtcag ctggcgaaag cgataccagg
      601 ctttaagccg cgagaaccac agcgacagat ggcggtagcc gtcacccagg cgatagaaaa
      661 aggccagccg ctggtggtgg aagcaggaac cggtacgggc aaaacctacg cttacctggc
      721 tcctgcgctg cgggcgaaaa agaaagtcat tatctcgacc ggctcaaaag cgttgcagga
      781 tcagctctac agccgcgatt tgccaacagt ctcaaaggca ttgaaatata cgggcaacgt
      841 ggcgctgctg aaagggcgct caaactacct ctgcctcgaa cgtctcgaac agcaggcgct
      901 ggcggggggc gatctgccgg tacaaatctt aagcgatgtg atcctgctgc gctcctggtc
      961 taatcaaata gtcgatggtg atatcagcac ctgcgtcagc gtggcggaag attcacaggc
     1021 gtggccgctg gtcaccagca ccaacgacaa ctgtcttggc agcgactgcc cgatgtataa
     1081 agattgcttt gtggtcaaag cacgtaaaaa agcgatggac gccgatgtgg tggtggtaaa
     1141 ccatcatctc tttctggcgg atatggtggt taaagagagt ggatttggcg agttgatccc
     1201 tgaagcggac gtcatgatct tcgacgaagc ccaccagcta ccggacattg ccagccagta
     1261 ttttggtcag tcactctcca gtcgacaact gctcgacctg gcaaaagaca tcaccatcgc
     1321 ctaccgcacc gaattaaaag acacccagca gttacaaaag tgcgctgatc gtcttgccca
     1381 gagtgcgcag gattttcgtc tgcaactcgg tgagccaggt tatcgcggta acctgcgtga
     1441 gctgttagct aatccgcaaa ttcagcgggc atttttactg ctcgatgaca ccttggaact
     1501 ttgttatgat gtggcgaaac tgtcactggg gcgttccgcc ttgctggatg cggcatttga
     1561 gcgcgccacg ttgtatcgca cacggctgaa gcggctaaaa gagatcaatc agccgggcta
     1621 cagctactgg tacgaatgca cttcgcgcca ttttactctg gctctcacgc cgctcagcgt
     1681 ggcggataaa ttcaaagagt taatggcgca aaagcccggt agctggatct tcacctcagc
     1741 aacgctgtcg gtgaacgacg atctgcatca tttcacctcg cggcttggga tagaacaggc
     1801 ggagtcgttg ctattgccaa gcccgtttga ttacagccgc caggcgttac tctgtgtgcc
     1861 gcgcaatctg ccgcaaacca accagccagg ttctgctcgc cagttagcgg caatgctgcg
     1921 accgatcatc gaagctaaca acggtcgttg ttttatgctt tgtacctcgc acgccatgat
     1981 gcgcgatctg gccgagcagt tccgcgctac catgacgctt cccgttttgt tgcaggggga
     2041 aaccagtaaa ggccaactgt tgcagcagtt tgtcagtgcc ggtaatgcgc ttcttgtggc
     2101 aaccagtagt ttctgggaag gggtggacgt gcgtggcgat acattgtcat tggtaattat
     2161 cgacaaattg ccgtttacct cgccggatga tccactgtta aaagcgcgca tggaagattg
     2221 tcgtttgcgc ggtggcgacc cgttcgatga agtgcaacta ccagatgccg tcattactct
     2281 caaacagggg gtaggtcgac tgattcgcga cgccgacgat cgtggcgtgc tggtgatttg
     2341 tgactatcgg ctggtgatgc gtccttacgg cgcgacgttt ctcgccagtc tgccgcccgc
     2401 gccacgcacc cgtgacattg cccgtgcggt tcgtttcctt gcgataccat cctccaggta
     2461 atttgttacg gagtatggta tgatgcgcgc ctttttattg atctaagcac gaagacccat
     2521 gcgaattctg gctatcgata ccgcgacaga ggcctgctct gtcgccctgt ggaacgacgg
     2581 tactgtcaac gctcattttg agctttgccc tcgtgaacat actcaacgaa tcttaccgat
     2641 ggtgcaggat atcctgacca ccagcggaac ttccctgact gatattaacg ctctggctta
     2701 cgggcgcggc cccggtagct ttaccggcgt gcgcattggt attggcatcg cgcaagggct
     2761 ggcgcttggc gcggaattac cgatgattgg cgtctccacg ctaatgacga tggcgcaagg
     2821 ggcgtggcgc aaaaacggcg caacacgcgt gctgtcagcc attgatgcgc gaatgggcga
     2881 agtttactgg gccgaatatc agcgtgatga aaacggtatc tggcacggtg aagaaaccga
     2941 agccgtactc aaacccgaac tcgttcatga acgaatgcaa cagctttccg gcgaatgggt
     3001 gacggtaggg acgggctggc aagcctggcc ggatctcggt aaagagagcg ggctggtttt
     3061 gcgcgatggc gaagtgttac tccccgctgc agaagatatg ctgccgattg cttgtcagat
     3121 gtttgccgag ggtaaaacgg tggcggtgga acatgccgag ccggtttatt tgcgtaacaa
     3181 cgtcgcatgg aagaaacttc cgggcaaaga atgaatctta agagtatgcc ctgagaaaaa
     3241 aaggagtcgc atcatggcgg ttcaaaagaa tgttatcaaa ggcatactgg caggtacgtt
     3301 tgcgctaatg ctgagcggtt gtgtcactgt gccggacgcc attaaaggca gcagtaccac
     3361 gccgcaacaa gatttagttc gggtgatgag tgcgccgcag ctgtacgttg gtcaggaggc
     3421 acgctttggt ggcaaagtgg ttgcggtaca aaaccagcaa gggaaaaccc gcctggaaat
     3481 tgctaccgta ccgctggaca gcggagctag gccgacgctg ggagaacctt ctcgcggtcg
     3541 catttatgcc gatgtgaacg gttttctgga cccggtggat ttccgtggac aactggttac
     3601 ggtagtcggg ccaatcaccg gtgcggttga cggcaaaatc ggcaatacgc cctataaatt
     3661 tatggtgatg caagtaacgg gttacaaacg ttggcattta acccagcagg tgattatgcc
     3721 gcctcagccg attgatccat ggttttatgg cggtcgtggc tggccctatg gctacggcgg
     3781 atggggctgg tataatcccg gccccgcgag agtacaaaca gttgtaaccg aataattgct
     3841 tgtttttaaa gaatgcagaa acagcggctg gtccgctgtt tctgcattct tacggtaaag
     3901 ataaaaataa atagtgacgc gcttcgcaac cttttcgttg ggtaattatc aagctggtat
     3961 gatgagttaa tattatgtta acggcgtgta tatcatttgg ggttgcgatg acgacgaaca
     4021 cgcattttag aggtgaagaa ttgaagaagg tttggcttaa ccgttatccc gcggacgttc
     4081 cgacggagat caaccctgac cgttatcaat ctctggtaga tatgtttgag cagtcggtcg
     4141 cgcgctacgc cgatcagcct gcgtttgtga atatggggga ggtgatgacc ttccgcaagc
     4201 tggaagaacg cagtcgcgcg tttgccgctt atttgcaaca agggttgggg ctgaagaaag
     4261 gcgatcgcgt tgcgttgatg atgcctaact tattgcaata tccggtggcg ctatttggca
     4321 ttttgcgtgc cgggataatc gtcgtaaacg ttaacccgtt gtataccccg cgtgagcttg
     4381 agcatcagct taacgatagc ggcgcatcgg cgattgttat cgtgtctaac tttgcccaca
     4441 ctctggaaaa agtggttgat aaaaccgccg ttcagcacgt aattctgacc cgtatgggcg
     4501 atcagctatc tacggcaaaa ggcacggtag tcaatttcgt tgttaaatac atcaagcgct
     4561 tggtgccgaa ataccatctg ccagatgcca tttcatttcg tagcgcactg cataacggct
     4621 accggatgca gtacgtcaaa cccgaactgg tgccggaaga tttagccttc ctgcaataca
     4681 ccggcggcac cacaggtgtg gcgaaaggcg cgatgctgac tcaccgcaat atgctggcga
     4741 acctggaaca ggtcaacgcc acctatgggc cgttgctgca tccaggtaaa gaactggtag
     4801 taacggcact gccgctgtat cacatttttg ccctgaccat taactgcctg ctgtttatcg
     4861 aactgggtgg gcagaacctg cttatcacta acccgcgtga tattccaggg ttggtaaaag
     4921 agttagcgaa atatccgttt accgctatca ctggcgttaa caccttgttc aatgcgttgc
     4981 tgaacaataa agagttccag cagctggatt tctccagtct gcatctttcc gcaggcggtg
     5041 ggatgcctgt tcagcaagtg gtggcagaac gctgggtgaa actgaccgga cagtatctgc
     5101 tggaaggtta tggcctgacc gagtgcgcgc cgctggtgag cgttaacccg tatgatattg
     5161 attatcatag cggtagcatc ggtttgccgg tgccatcgac ggaagccaaa ctggtggatg
     5221 acgacgacaa cgaagtacca ccgggtcaac cgggtgagct ttgtgtcaaa ggaccgcagg
     5281 tgatgctggg ttactggcag cgtccggatg ctacagatga gatcatcaaa aatggctggt
     5341 tacacaccgg cgacatcgcg gtgatggatg aagaaggatt cctgcgcatt gtcgatcgta
     5401 aaaaagacat gattttggtt tccggtttta acgtctatcc caacgagatt gaagatgtcg
     5461 tcatgcagca tcctggcgta caggaagtcg cggctgttgg cgtaccttcc ggctccagtg
     5521 gtgaagcggt gaaaatcttc gtagtgaaaa aagatccatc gcttaccgaa gagtcactgg
     5581 tgactttttg ccgccgtcag ctcacgggat acaaagtacc gaagctggtg gagtttcgtg
     5641 atgagttacc gaaatctaac gtcggaaaaa ttttgcgacg agaattacgt gacgaagcgc
     5701 gcggcaaagt ggacaataaa gcctgagcgt taagtcagtc gtcagacgcc ggttaatccg
     5761 gcgttttttt tgacgcccac taaagagaaa acaatttgaa ttaccaaatg attaccacgg
     5821 acgatgcgct ggcttctttg tgtgaagccg tccgtgtctt tccggcgata gccctggata
     5881 ctgaatttgt tcgtacgcgc acttattacc cgcagctggg gttgattcaa cttttcgatg
     5941 gcgagcatct ggcgctaatt gatcctctcg ggatcaccga ctggtcaccg ctgaaagcga
     6001 tcctgcgcga tccgtccatc acaaaatttc tccatgcagg cagtgaagat ctggaagtgt
     6061 tcctcaatgt ctttggcgaa ttaccacaac ccttgattga cacgcaaatc cttgctgcct
     6121 tctgcggacg cccgatgtca tggggtttcg cttccatggt ggaagagtat tccggcgtta
     6181 cgctggacaa gagtgaatcg cgcaccgact ggctggccag accgctgacc gaacgtcagt
     6241 gtgaatacgc agcggcggat gtctggtatc tgttaccgat caccgccaag cttatggtag
     6301 aaacggaggc ctccggctgg ctacctgcgg cgctggatga atgccgcctg atgcaaatgc
     6361 gtcgtcagga agtcgttgcg ccggaagatg cctggcgtga tatcaccaat gcctggcagt
     6421 tacgcacacg ccaactggcc tgtctgcaac tgttagccga ctggcgactg cgcaaggcgc
     6481 gagagcgtga tctggcggtg aactttgtcg tgcgtgaaga gcatttgtgg tcggtcgcgc
     6541 gttatatgcc gggaagttta ggcgaactgg acagcctggg tttatccggt agcgaaatcc
     6601 gctttcacgg taaaacgctg ctggcgctgg tggaaaaagc gcagacattg ccggaagatg
     6661 ccttaccgca gccgatgctt aacctgatgg acatgcctgg ttatcgtaaa gcgtttaaag
     6721 cgattaagtc gctgattact gacgtgagcg aaacgcataa gatcagcgcc gaattgctgg
     6781 catcgcgtcg gcaaatcaac caactgctga actggcactg gaaactgaaa ccgcagaaca
     6841 atttgccgga gctgatttcc ggctggcgtg gtgagctgat ggcggaagca ttacacaatt
     6901 tattgcagga atatccgcag taaaatcttc cgaagccgga ctgggcgcgc tcagcgccac
     6961 atccggcttt ggcaaactac aaatccaaca ccaggcgttt acccttcgca cgcgaacaac
     7021 agatcaacat actttgctgg ctggcacgct cttcatcgct aaaatactga tcccgatggt
     7081 cagcttcacc ttccagtatt gccgtttcgc aggttccgca taccccttca cgacataaac
     7141 attccacttt cgcggcttta ttattttcaa taacctgcaa aatagtcatc tcttccggca
     7201 ccacaaactc ttttccggaa cgggcaagca ccagggtaaa tgcatcgccg gttttgtctt
     7261 cgatagcaaa ttgctcaaag tgcagtgtat cggcggcgat gtccagacgc gcagcttcac
     7321 ttcttaccgc ttcaattagc gcctcggggc cacaggtata aacgtgtgtg ccaggttcga
     7381 tatccgccaa taatcgcgct aattccagtc gtgttccggt tgatgaatga tgcaaatgga
     7441 ctttctcagc ctgcggatgc tggactagct cagcacgaaa tgcgcaactt tctggatttc
     7501 gcgagcagta atgtagctgc cagtcgatgt cgctgtgttg caactctgcc atgtgcgaca
     7561 ggaaaggggt gataccaata ccgcccgcga taaacagatg ctttctggct gagggaatta
     7621 gcgcaaacag gttattaggc gttgagatcg ttaaccgatc gcccactttt acctgctgat
     7681 gcaaaaagcg ggaaccgccg cgcgagtttt cttccagtcg aacggcaatc tgataactag
     7741 aggtgttatg cggtgaactc agtagtgaat acgcattgct gtactggtta tcaccatcgc
     7801 tcatctgcac taggatgtga cttcctccgg tgaacgcagg cagcggtttg ccatcggttg
     7861 ccaccagcgt gaagcgtttc acctgttcgg taaggggttc aacctggctc acctgtactt
     7921 caaacatttg atagtctgac attgctgtac tccatcaaaa atcagcccag cggcggaaaa
     7981 taccgccgcc gatgtcatta gtccttaaac acctgcgcca gcagattatg gaaatgggcg
     8041 ataccatgtt cggaaatgcc actaccgcta ctgtcggcca tgatgcgccc ctgaccacga
     8101 tagccacgcg atttcagccc tttctgtacg ctttcaacca gacgtaaatc ttccggatgg
     8161 aacacatcgc gataccactc aatcagcgat ttttgctcgt cggttaactc ttcattggtg
     8221 aagtaaatat cgtagttttg cagggtagtt tcagaatcca ccgggaattc ataaatgacc
     8281 gtcatcatcc ctttgatcgg ggtgacgttc agcatcgtgc acggccacag ccagaaaccg
     8341 tggaatgccg catccgtacc ctcttcaaat ttaaacgact gttcggacgg tttggcgaaa
     8401 ccgtattgca gcgtccagtt accgtgcatg gtgtgccagt aacgatcaac ctgtacggag
     8461 tcggagaaac ctagatgcgc cggaccacag tgatagcact cgagatagtt atcgacaatg
     8521 ttcttccagt tggcaggcgt gcgggtggta aagcgggccg ctagtttcag attgtggact
     8581 tccgggcagg cttccagcac tttcgcgccc agaccggtgt attgattcac ttgaagtacg
     8641 aaaaaaaacc gggaggacat tggattattc gggttctgat gggattagat ttggtggggc
     8701 ttgcaagcct gtagcgcaaa ttttagtcgt taatcaatga aacgcgaaag atagtaaaaa
     8761 attgcttttg tttcattgaa aatacgaaaa acaaaaacac tgcaaatcat ttcaataaca
     8821 gcttcaaaaa acgttcaaaa ccgataacaa ccaggctgta accaaatgac tcatatcaca
     8881 aatcagctca tgccgtttag gtatgttaca tgtgtgatta tgtgaggtga agtatgtttt
     8941 agctggttca tggttgttat acggcttttt ttacatcttg tggttcttgt gaaggagcta
     9001 caagaccttc ctgttcataa ataccatact ttgacaaaat ctttttacga gtatctgttg
     9061 gtaatgattc aagcattttg gctgtttgta aggtttgctg cgaaatacct tgcaaatcag
     9121 cctggatgga atcatcggaa gaaaaaatct caggcataag agcttcaaat cctttctttc
     9181 tgaacaactc aatcgcagaa tgcagttcct tgtcctctaa caattcgaca attgcgatca
     9241 gtttagtttt caatgtgctg cggtcaggtt gagaattttt attctcttga ttatttttat
     9301 gctcaaccat tggcatgctc tgactttgga taaaccactc tattggacgc cctgttgcgt
     9361 aggctatttg tgctaatcga tctaaagacg ggtatgtctt gccagacagg tagtttctga
     9421 taaccccatc agaaagtccg cacttagctg caaacgcccg gcttgattct ccattcattg
     9481 cctcttcaag gcgttccttg aaacgcatta tttccggggg cataaaaatg cgctctgcat
     9541 ttttgctttc tttttcacct gtcatgccca atctctctgt tatataaaga attttacaga
     9601 attctttgat ttgcactggt taaaataaaa atgcgcatag cttttgaatt gagcattttt
     9661 ctgcgctatc gtttttcccg atagcggtag ccggtatcag ttaccgaaaa cgttaagaga
     9721 gtaaggatcg cagaatgtca gcaaaaaacc aagactggca cgcagagcag ataaaggctg
     9781 ctgttcgtat gaaggggaag acactatctc aattatccag ag
//
LOCUS       DAZSNO010000144         9772 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZSNO010000144 DAZSNO010000000
VERSION     DAZSNO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.144"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            390..2021
                     /gene="oppA"
                     /locus_tag="ACHWN2_003279"
     CDS             390..2021
                     /gene="oppA"
                     /locus_tag="ACHWN2_003279"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR8932695.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2107..3027
                     /gene="oppB"
                     /locus_tag="ACHWN2_003280"
     CDS             2107..3027
                     /gene="oppB"
                     /locus_tag="ACHWN2_003280"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR8932696.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3042..3950
                     /gene="oppC"
                     /locus_tag="ACHWN2_003281"
     CDS             3042..3950
                     /gene="oppC"
                     /locus_tag="ACHWN2_003281"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR8932697.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3962..4975
                     /gene="oppD"
                     /locus_tag="ACHWN2_003282"
     CDS             3962..4975
                     /gene="oppD"
                     /locus_tag="ACHWN2_003282"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR8932698.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4972..5976
                     /gene="oppF"
                     /locus_tag="ACHWN2_003283"
     CDS             4972..5976
                     /gene="oppF"
                     /locus_tag="ACHWN2_003283"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR8932699.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6029..6358)
                     /locus_tag="ACHWN2_003284"
     CDS             complement(6029..6358)
                     /locus_tag="ACHWN2_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR8932700.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6393..7853)
                     /gene="cls"
                     /locus_tag="ACHWN2_003285"
     CDS             complement(6393..7853)
                     /gene="cls"
                     /locus_tag="ACHWN2_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR8932701.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7996..8169
                     /locus_tag="ACHWN2_003286"
     CDS             7996..8169
                     /locus_tag="ACHWN2_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR8932702.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8224..9477)
                     /gene="kch"
                     /locus_tag="ACHWN2_003287"
     CDS             complement(8224..9477)
                     /gene="kch"
                     /locus_tag="ACHWN2_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR8932703.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2285 c         2432 g         2492 t
ORIGIN      
        1 aaagatgttt gttaaggcat ggatgcaagc tatagattct gatacggtca ataaaagaga
       61 atcgcttaac aattttgcaa aatgtattgg cgagtaagaa ccgcatttgg tactttccgg
      121 gcaaccgcca gacgattctt tattggtaat gagaataatt aacaattaaa gagcgtcgcg
      181 aaagaataat gtgtctcgac aggggagaca cagtacgaat cgacataagg tgatcgtctg
      241 aatcaccaga ataaataaag tcggtgatag taatacgtaa cgataaagta acctgacagc
      301 agaaagtctc cgagcttgtg cagggtccca atccgggatt acacatgctg gttaatacca
      361 gtaattataa tgagggagtc caaaaaacaa tgaccaacat caccaagaga agtttagtag
      421 cagctggcgt tctggctgcg ctaatggcag ggaatgtcgc gctggcagct gatgtacccg
      481 caggcgtcac actggcggaa aaacaaacac tggtacgtaa caatggttca gaagttcagt
      541 cattagatcc gcacaaaatt gaaggtgttc cggagtctaa tatcagccga gacctgtttg
      601 aaggcttact ggtcagcgat cttgacggtc atccagcacc tggcgtcgct gaatcctggg
      661 ataataaaga cgcgaaagtc tggaccttcc atttgcgtaa agatgcgaaa tggtctgatg
      721 gcacgccagt cacagcacaa gactttgtgt atagctggca acgttctgtt gatccgaaca
      781 ctgcttctcc gtatgccagt tatctgcaat atgggcatat cgccggtatt gatgaaattc
      841 ttgaagggaa aaaaccgatt accgatctcg gcgtgaaagc tattgatgat cacacattag
      901 aagtcacctt aagtgaaccc gttccgtact tctataaatt acttgttcac ccatcaactt
      961 caccggtgcc aaaagccgct atcgagaaat tcggcgaaaa atggacccag cctggtaata
     1021 tcgtcaccaa cggtgcctat accttaaaag attgggtcgt aaacgaacga atcgttcttg
     1081 aacgcagccc gacctactgg aacaacgcga aaaccgttat taaccaggta acctatttgc
     1141 ctattgcttc tgaagttacc gatgtcaacc gctaccgtag tggtgaaatc gacatgacgt
     1201 ataacaacat gccgatcgaa ttgttccaga agctgaaaaa agagatcccg gacgaagttc
     1261 acgttgatcc atacctgtgc acttactatt acgaaattaa caaccagaaa ccgccattca
     1321 acgatgtgcg tgtgcgtacc gcactgaaac taggtatgga ccgcgatatc attgttaata
     1381 aagtgaaagc gcagggcgac atgcccgcct atggttatac tccaccgtat actgatggcg
     1441 caaaattgac tcagccagaa tggtttggct ggagccagga aaaacgtaac gaagaagcga
     1501 aaaaactgct ggctgaagcg ggttataccg cagacaaacc gttgaccatc aacctgcggt
     1561 ataacacctc cgatctgcat aaaaagctgg cgattgctgc ctcttcattg tggaagaaaa
     1621 acattggtgt aaacgtcaaa ctggttaacc aggagtggaa aacgttcctc gacacccgtc
     1681 accagggcac ttttgatgtg gcccgtgcag gctggtgtgc agactacaac gaaccaactt
     1741 ccttcctgaa caccatgctg tcgaacagct cgatgaatac cgcgcattat aagagcccgg
     1801 cctttgacag cattatggcg gaaacgctga aagtgactga cgaggcgcag cgcacagctc
     1861 tgtacactaa agcagaacaa cagctggata aggattcggc cattgttcct gtttattact
     1921 acgtgaatgc gcgtctggtg aaaccgtggg ttggtggcta taccggcaaa gatccgctgg
     1981 ataataccta tacccggaat atgtacattg tgaagcacta atggcaatac gtggggcagg
     2041 agtgtcctgc tccacggtgt ctgattttta tcgcattaca gaaggcacag gccagaaggt
     2101 agggcaatgt taaaatttat tctacgtcgt tgtctggaag cgattccgac gctatttatt
     2161 cttattacta tttcgttctt tatgatgcgc ctcgcaccgg gaagcccttt taccggcgaa
     2221 cgtactttac cgccagaagt gatggccaat atcgaagcga aatatcatct taatgatccg
     2281 atcatgacac agtatttcag ctacctgaaa caactggcgc atggcgattt cggtccatcg
     2341 tttaaatata aagattattc ggtcaatgac ctggtggcat ccagttttcc cgtttctgcc
     2401 aaactgggag ccgccgcatt tttccttgcg gtaatactgg gtgttagtgc tggcgttatt
     2461 gccgcattaa aacaaaacac caaatgggac tataccgtga tggggctggc aatgaccggg
     2521 gttgttatcc ccagttttgt ggttgcgcca ttattagtca tgatatttgc gatcattttg
     2581 cattggctgc cgggcggtgg ctggaatggt ggggcgctta aattcatgat attgccgatg
     2641 gtggcgttgt cactcgccta tatcgccagt attgcgcgta ttacccgtgg ctctatgatt
     2701 gaagtattac actccaactt tattcgtact gcccgggcga aagggttacc tatgcggcgg
     2761 atcattttac gccatgcatt aaaacctgct ctgttacccg tgctctccta tatgggcccc
     2821 gcatttgtcg gcattattac cggttctatg gtcatcgaaa ccatttatgg tttgccgggg
     2881 attgggcaac tgttcgttaa tggcgcattg aaccgtgact attccttagt gttaagcctg
     2941 accatcctgg ttggtgcgtt aaccattttg tttaatgcca ttgtcgatgt gctatatgcg
     3001 gttatcgacc cgaaaatccg ttactgatac tggagctcgc gatgatgtta agtaagaaaa
     3061 acagcgagac gctggaaaat ttcagtgaaa agctggaggt cgaagggcgc agcttgtggc
     3121 aggacgcacg tcgacgtttt atgcataacc gtgcggcggt tgccagtctg atagtgctgg
     3181 tgctgatcgc gttatttgta atcctggcac cgatgctttc ccagtttgcc tatgacgata
     3241 ctgactgggc gatgatgtcc agcgccccgg atatggagtc cggtcactac tttggtactg
     3301 actcatccgg tcgcgacctg cttgtgcgcg ttgcgattgg cgggcgtatc tcactcatgg
     3361 tcggtgttgc tgcggcactg gtggcagtgg tcgtggggac actttatggt tcgctttccg
     3421 gttatctggg cggtaaagtg gattcggtaa tgatgcgtct gctggaaatc ctcaactcct
     3481 tcccattcat gttcttcgtc attttgctgg tgaccttttt cggccaaaac atcctgctga
     3541 ttttcgtggc gattggcatg gtttcctggc tggatatggc tcgtattgtg cgtgggcaaa
     3601 ccctgagtct gaagcgcaaa gagtttattg aggcggcaca agttggcggt gtatcgacgc
     3661 cgggcattgt tattcgccac attgtgccga acgtactcgg tgtggtggtg gtctacgcat
     3721 cgctactggt gcccagcatg atcctctttg aatctttcct tagcttcctg gggttgggta
     3781 cgcaagagcc gttaagcagc tggggggcat tgctgagtga tggcgcgaac tcgatggaag
     3841 tctctccatg gttactgttg ttcccagcgg gattccttgt ggtgacactg ttttgtttca
     3901 actttatcgg cgatggcttg cgtgatgccc tcgacccgaa agatcgttaa ggagtgcagc
     3961 catgagcgta attgaaactg caactgtgcc gctcgcacaa caacaggctg acgcactgct
     4021 gaacgtgaaa gatttgcgtg tcacctttag taccccggac ggcgacgtca cggcggtcaa
     4081 tgatttaaat ttttccctac gtgccggaga aacgctgggc attgtaggtg agtctggttc
     4141 gggtaaatcg caaactgcat ttgcgttgat gggcctgttg gctgccaacg gacgcattgg
     4201 cggatcggca accttcaatg ggcgtgaaat cctcaatttg ccagagcgtg aactcaataa
     4261 actgcgcgct gaacaaatct caatgatttt tcaggaccca atgacttcgt tgaatcccta
     4321 tatgcgcgtc ggtgagcagt tgatggaagt gctgatgctg cataagaaca tgagcaaagc
     4381 tgaggcgttt gaagagtcgg tgcggatgct cgatgcggta aaaatgccgg aagcacgtaa
     4441 acgcatgaaa atgtacccgc acgaattttc tggcggcatg cgtcagcgag tcatgattgc
     4501 gatggcattg ctatgtcgac ctaagttgct gattgcggat gaacccacta cagcgctgga
     4561 cgtcaccgta caggcgcaga tcatgacgct attgaatgaa ctgaagcggg aatttaatac
     4621 tgccatcatt atgatcaccc acgatcttgg cgtggtggcg gggatctgtg acaaagtgct
     4681 ggtaatgtac gccgggcgca cgatggaata tggcaatgcg cgcgatgtct tttatcaacc
     4741 cgttcatcct tattctatcg gtttgctcaa cgcggtgccg cgtcttgatg cggaaggtga
     4801 aacaatgttg accatcccgg gtaatccgcc aaacttgctg cgattaccga aaggttgccc
     4861 gttccagcca cgttgtccac atgcgatgga aatttgtagt agcgcaccgc cgctggaaga
     4921 gtttacgcct ggccgtctgc gtgcttgctt taaaccggtg gaggaactgt tatgaatgct
     4981 gtaactgaag gaagaaaagt cctccttgaa atcgccgatc ttaaagtgca ctttgaaatc
     5041 aaagatggca aacagtggtt ctggcaaccg ccgaaaacgc tcaaagccgt cgatggtgtc
     5101 actcttcgcc tgtatgaagg ggaaacatta ggtgtggtag gggaatcggg atgcggtaag
     5161 tccacctttg ctcgcgccat catcggtttg gtcaaggcga ccgacggtca tgttgcctgg
     5221 ttaggtaaag agttgctggg catgaagccc gatgaatggc gtgccgttcg cagtgatatt
     5281 cagatgattt tccaggatcc gttggcatcg ctaaacccgc gtatgaccat cggcgagatc
     5341 atcgctgaac cactgcgtac ttatcatccg aaaatgtcac gccaggaagt tcgcgagcgc
     5401 gtgaaggcga tgatgctgaa agtcgggtta ttgcctaacc tgattaaccg ctatccgcat
     5461 gagttctccg gtgggcagtg ccagcgtatc gggattgctc gtgctcttat tcttgaaccg
     5521 aagctgatta tctgcgatga gccggtttcg gcgctggacg tgtcaattca ggcgcaggtg
     5581 gttaacctgc tccagcagct gcaacgtgag atgggattgt cattaatttt tattgctcat
     5641 gacctggccg tggtaaaaca catttccgat cgtgtgttgg tgatgtatct cggccatgca
     5701 gtagaactgg ggacctatga tgaggtctac cacaatccac tacatcctta caccagggca
     5761 ttgatgtcgg cagtccccat acctgatccg gatctggaga agaacaaaac catccagtta
     5821 ctggaagggg aattaccgtc gccgatcaac ccgccttccg gttgtgtttt ccgtacccgt
     5881 tgcccgattg ccggtccgga gtgcgccaaa acacgtcctg ttctggaggg gagtttcaga
     5941 cacgccgttt cttgcctgaa agtcgatccg ctttaaaaac aataagggct gacagttgtc
     6001 agcccttttt cacgctaaaa gcgattattt attcccgcca gatgatatgg caaagtttgt
     6061 gatctttttc gcggcataac agaatgcggg cgaaaacatc gctgatttca ccatcttcac
     6121 tgtccgccag accaatcacc acttcggcaa aaaagtccgg gttcaaatcg aaatcaacat
     6181 gttcctgcca gtcttccgcc ggatcgaata actccgcgcc gccgcgctct tcaaactgaa
     6241 gattgaacag cagaacatca gctggatcga gattgtcggc agccagttcg agaaaaatat
     6301 cgtaggcctg ctcaagcgtt tcatcttcag tcaggcgatt gtttagatcc atatccatag
     6361 tcactacctg tttaacctct gttggcgacg ttttacagca acgggctgaa gaagtaaaac
     6421 agtcgctcgg cgacacgttg ccataatgga cgttttagcc ataaacgggc atcgagcaga
     6481 cgtgaacgcg aaatataatc gtcctgaacg gcggcaaggt ctgcaccaaa acctttatcg
     6541 tcgattgcca aggtaatctc gaaattcagc cacagactac gcatatcaag gttaacggtg
     6601 ccaaccagac ttagttcgcc atcgaccagc acgctcttgg tatgcagtaa cccgccttca
     6661 aactgataaa ttttaacccc agcagcaagc agttccgtaa agaatgcgcg actggcccag
     6721 ccgaccagca tcgagtcatt ttttcgcgga aggataatac tgacatccac cccgcgctgc
     6781 gccgccgtgc aaatcgcatg aagtaaatca tcgcttggca caaagtaggg cgtggtcatg
     6841 atcaaatatt catgcgccga ataagccgca gtcaataatg cctggtgaat gagatcttcc
     6901 ggaaagccgg ggccagaagc aattgtgtga atggtgtgac cgctggcctg ttcaaacggc
     6961 ataatattga catctggtgg tggcggcaga atacgttttc cggtttcaat ctcccagtcg
     7021 caggaataaa taatccccat cgcggtggcg atggggcctt ccatacgcgc catcagatca
     7081 atccattgcc ctacgcccgc atcttgtttg aagtagcgag gatcgaccat attcatgctg
     7141 ccggtgtacg cgatgtaatt atcgatcatg atcatcttgc gatgttggcg caggtccata
     7201 cggcgtaaaa acacacgcat cagattgacc tttaaggctt cgaccacttc aataccggca
     7261 ttacgcatta gctcgggcca cgggctgcgg aaaaaagcca cactcccggc ggagtcgagc
     7321 atcaatcggc aatgaatacc acgccgtgca gccgccatta atgattcagc cacctggtcc
     7381 gccatgccgc cgggctgcca gatataaaac accatctcaa tattatggcg cgcgagctgg
     7441 atgtcgcgga ttaacgcctg catcacatca tctgactcgg tcatcagctg tagctgattc
     7501 cctttgaccc cagcgatccc ctgacgacgc tcgcaaagct tgaataatgg cgcagcgaca
     7561 ctgctatttt cttcggcgaa gatatgctta caggctttaa ggtcgttaag ccattttgcg
     7621 gtggaaggcc acatcgctct ggcgcgctca gcgcggcgtt tgcctaaatg gagctcgcca
     7681 acggcaagat aggcaataat tccgactaac ggcagaatgt aaataatcaa cagccaggcc
     7741 atcgcggagg gaactgcgcg tcgtttcatt agaatgcgta aagttacgcc tgcaatgagc
     7801 aaccagtatc ccagaatggc caaccaactc accaacgtat aaacggttgt catagattag
     7861 aaatcctttt gaaagcgcat tgttatgagt ttacgcatat ggattcatct ggcaaataaa
     7921 aacgcgggaa aagcactggt cagcgtcggg ggcgggggta taatgaccat tctgttattg
     7981 catagagtag ttaacatgaa gcggagtaga acggaagtgg ggcgctggcg catgcagcgt
     8041 caggctagcc gacgtaaatc gcgttggctt gaggggcaat cgcgccgaaa tatgcgtatc
     8101 cacagcatca ggaagtgcat tctaaacaaa cagcgtaact cgttattgtt tgcgatctac
     8161 aatatctaaa tgtactgggc accattgcgg tgcccaataa gtttactctt ccgtctttaa
     8221 actctatttt tgcgccgatt ctttactgtc agctttcgtt tcttgttcat cgttatcgct
     8281 gaaaatacca tgacctgagt tcaacaacat tgaaacgagc atatcattat taatctcttc
     8341 accatttaaa actcgcgcca gaatttcgct gccaaacagt tgcggtgaga gaatgatatc
     8401 cggatgtacc atcttaatct tattcaggtt tttgctatca ctgacggcga gaactgtttt
     8461 gacatcactg ctcatatctt ttgccgagag tacaacaaac gcgttatctg catcgttatc
     8521 actcagcgcc agaatggccc ggcaacgatc gattcccgct ttctttaata ctgaactgtc
     8581 attactgtca ccggggataa catcagcgtt atcgcctaag cgttgctcaa gttgcttgat
     8641 atcatcttca ggcaagttgc tgataaccgt tacgttttgt ccgcgttgat tcagttgcag
     8701 gatcgtattg atggcgagaa tcgaatgtcc gcaaacaata aaatgatctt tacgatgcat
     8761 tgtatgattg tttcctttta aaagtttgtt gaatcccccg cggataagcg gaccaaaaat
     8821 tgaggtcata gatgtggcaa aaacggtaat gccggaaata atgaccgaaa tagtgaacaa
     8881 tcgtgctgat tcagaaacag ggacaatatc tccatagccg acggttgaca tggtttctat
     8941 cgaaaaatag aacgcggtca tcaaactttc tattcgcgga ttaaaacctt cgcttaaata
     9001 aagcgcaccg taggttgagt aaaacagtaa tgtcgtgaaa ctaataaatg caaaaattgt
     9061 cccggctgcg gcgctactgt gggagaagtc tttgcgcagt atcagcaaaa acaccagcgt
     9121 aaaaatgcaa aatccaatac taaatttcag ccagggataa aaatgcaggg tgtaaattag
     9181 cgctatcaac aacaaaatga tactgattgc ccatgcgagc tttgcccgga acagtaagcc
     9241 aatggagttc aggacaagaa agacgccgag cataaatagt ggggcgtttg ccagcaagga
     9301 gagatccaac tctgaaaatg ctttgatatg gaaaatatca agcaaattga ccgacatacc
     9361 atagattgtt ttaaaaataa gcaatccatt taaaaagacg gccagcgcga gaatgtcgtg
     9421 ccgtagggtc acccataaat ttgttgctgt ttgtttgaat gtagcccagt gactcactga
     9481 tacctctgta accacaattt tgtttaatta tcttagcgtg cacattttct gaacatacat
     9541 gcagcgtgtg aatgtgttaa acaatagtag atgactttat ccgcagtaat caaaggtttt
     9601 gagtgagcaa ctctgcgtca ggaaagggct tttttcctga acatgctcac atcatataaa
     9661 gaaatacaga taaagttatt atctgcttgt ggtagtgaat gcactgaccg gctataagga
     9721 aaggccaaac aagaacacgg ttgcaaaaac cgtgccctta aatattggat ct
//
LOCUS       DAZSNO010000145         9684 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZSNO010000145 DAZSNO010000000
VERSION     DAZSNO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.145"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHWN2_003288"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHWN2_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR8932704.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="ACHWN2_003289"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="ACHWN2_003289"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFR8932705.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="ACHWN2_003290"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="ACHWN2_003290"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR8932706.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="ACHWN2_003291"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="ACHWN2_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR8932707.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="ACHWN2_003292"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="ACHWN2_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR8932708.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="ACHWN2_003293"
     CDS             4025..4615
                     /locus_tag="ACHWN2_003293"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR8932709.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="ACHWN2_003294"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="ACHWN2_003294"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR8932710.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="ACHWN2_003295"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="ACHWN2_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR8932711.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="ACHWN2_003296"
     CDS             complement(6551..6658)
                     /locus_tag="ACHWN2_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFR8932712.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHWN2_003297"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHWN2_003297"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR8932713.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="ACHWN2_003298"
     CDS             7648..7875
                     /locus_tag="ACHWN2_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR8932714.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHWN2_003299"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHWN2_003299"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR8932715.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..>9684)
                     /locus_tag="ACHWN2_003300"
     CDS             complement(9447..>9684)
                     /locus_tag="ACHWN2_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932716.1"
                     /translation="LAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGN
                     HYDVYLPPELFSQAYKLKNKEMNAQLDYLNRYAT"
BASE COUNT         2205 a         2522 c         2559 g         2398 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 agcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aaga
//
LOCUS       DAZSNO010000146         9681 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZSNO010000146 DAZSNO010000000
VERSION     DAZSNO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.146"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(44..214)
                     /locus_tag="ACHWN2_003301"
                     /pseudo
     CDS             complement(44..214)
                     /locus_tag="ACHWN2_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHWN2_003302"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHWN2_003302"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR8932717.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="ACHWN2_003303"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="ACHWN2_003303"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR8932718.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="ACHWN2_003304"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="ACHWN2_003304"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR8932719.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="ACHWN2_003305"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="ACHWN2_003305"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR8932720.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="ACHWN2_003306"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="ACHWN2_003306"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR8932721.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="ACHWN2_003307"
     CDS             5109..6242
                     /locus_tag="ACHWN2_003307"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR8932722.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="ACHWN2_003308"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="ACHWN2_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR8932723.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="ACHWN2_003309"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="ACHWN2_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR8932724.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="ACHWN2_003310"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="ACHWN2_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR8932725.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="ACHWN2_003311"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="ACHWN2_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR8932726.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="ACHWN2_003312"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="ACHWN2_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR8932727.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="ACHWN2_003313"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="ACHWN2_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR8932728.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2480 c         2466 g         2270 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt t
//
LOCUS       DAZSNO010000147         9652 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZSNO010000147 DAZSNO010000000
VERSION     DAZSNO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.147"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..401
                     /locus_tag="ACHWN2_003314"
                     /pseudo
     CDS             1..401
                     /locus_tag="ACHWN2_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="ACHWN2_003315"
     CDS             484..741
                     /locus_tag="ACHWN2_003315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932729.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="ACHWN2_003316"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="ACHWN2_003316"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR8932730.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="ACHWN2_003317"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="ACHWN2_003317"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR8932731.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="ACHWN2_003318"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="ACHWN2_003318"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR8932732.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="ACHWN2_003319"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="ACHWN2_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR8932733.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPTWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="ACHWN2_003320"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="ACHWN2_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR8932734.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="ACHWN2_003321"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="ACHWN2_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR8932735.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="ACHWN2_003322"
     CDS             complement(8323..8844)
                     /gene="fecI"
                     /locus_tag="ACHWN2_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR8932736.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8981..9049
                     /gene="ykiE"
                     /locus_tag="ACHWN2_003323"
                     /pseudo
     CDS             8981..9049
                     /gene="ykiE"
                     /locus_tag="ACHWN2_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
     gene            9318..9422
                     /locus_tag="ACHWN2_003324"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="ACHWN2_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2201 a         2624 c         2705 g         2122 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggt aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaacgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggt tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tg
//
LOCUS       DAZSNO010000148         9539 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZSNO010000148 DAZSNO010000000
VERSION     DAZSNO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9539
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.148"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(58..960)
                     /locus_tag="ACHWN2_003325"
     CDS             complement(58..960)
                     /locus_tag="ACHWN2_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFR8932737.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(1098..2339)
                     /gene="yihS"
                     /locus_tag="ACHWN2_003326"
     CDS             complement(1098..2339)
                     /gene="yihS"
                     /locus_tag="ACHWN2_003326"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFR8932738.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2356..3234)
                     /gene="yihT"
                     /locus_tag="ACHWN2_003327"
                     /pseudo
     CDS             complement(2356..3234)
                     /gene="yihT"
                     /locus_tag="ACHWN2_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
     gene            complement(3258..4154)
                     /gene="yihU"
                     /locus_tag="ACHWN2_003328"
     CDS             complement(3258..4154)
                     /gene="yihU"
                     /locus_tag="ACHWN2_003328"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR8932739.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4322..5218
                     /gene="yihV"
                     /locus_tag="ACHWN2_003329"
     CDS             4322..5218
                     /gene="yihV"
                     /locus_tag="ACHWN2_003329"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFR8932740.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5252..6037
                     /locus_tag="ACHWN2_003330"
     CDS             5252..6037
                     /locus_tag="ACHWN2_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR8932741.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6136..6735
                     /gene="yihX"
                     /locus_tag="ACHWN2_003331"
     CDS             6136..6735
                     /gene="yihX"
                     /locus_tag="ACHWN2_003331"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR8932742.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6729..7601
                     /locus_tag="ACHWN2_003332"
     CDS             6729..7601
                     /locus_tag="ACHWN2_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR8932743.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7598..8035
                     /gene="dtd"
                     /locus_tag="ACHWN2_003333"
     CDS             7598..8035
                     /gene="dtd"
                     /locus_tag="ACHWN2_003333"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR8932744.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8080..9021
                     /gene="fabY"
                     /locus_tag="ACHWN2_003334"
                     /gene_synonym="yiiD"
     CDS             8080..9021
                     /gene="fabY"
                     /locus_tag="ACHWN2_003334"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR8932745.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            9437..>9539
                     /locus_tag="ACHWN2_003335"
     CDS             9437..>9539
                     /locus_tag="ACHWN2_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932746.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLN"
BASE COUNT         2252 a         2376 c         2525 g         2386 t
ORIGIN      
        1 tgttgccttt agcgagtgaa atgttcttca atgattttga ctgctaatca atacaagtta
       61 ttttctctgt ccgtaaatat taaaaaacag gcggtgcggt tttcctggtt ccagtaataa
      121 taagtcgata cctgaattaa aggcgttagg cgggcaactc ataggttcaa ctgccagtcc
      181 ctgacgctgt aatttttcac cgctataaac ctgcacccag ggttgatcgg aacaaaggct
      241 taccgaaagc gcctgctgtg gatgggtaat ggtcatttcc cacaaatcat tagcggtttt
      301 aaaggtgtgg tcgatttttg tcgcggctat ttttttcgcc tgcgtgaaat ttaaatccag
      361 ctcatcaacg tggtgaagcg ttgtcggatt tacgtgttca tcgacggcat aaacctggtt
      421 tgccggaagc tggaacagat attcatcaac agatgttaag ttgcaggtta aataaggatg
      481 aatacccacg ccataaggcg ctgcgacagt gccgatattc tggcttgcga tctcaacgga
      541 aagacccgtg tgtgcgttga gcgaataaac aacttgtgaa gccagcataa acggatagcc
      601 gtaactgggc ggcagaaaag ctgtcagggt aacgctggtg gtggttaatt cactaatttg
      661 ccagtctcgc caggcaagca gcccgtgaat agcggctttt gaactatgtt cattaatggg
      721 taactgatat tcctgcccct gataacggta acagccgttg gcgatccggt tcggccacgg
      781 aataagcact ttacccagat gcgccagcgg catctcttcg ggtttgtgcg ggatgactaa
      841 atgacaaccc tgaaaggtta attcagccag accagcaccg acggtcacaa tggtcgcgtg
      901 ataatccccc gcggcaagtg aaacggtctg tccactgcaa tgcatattgg tgatttgcat
      961 acaaggcacc ttaattaacg acatacagcc tcccgtgagg ctgatgtcaa acccaaaaac
     1021 tgcaccggac ggtcccctcg cccctttggg gagagggtta gggtgagggg aaaacccgca
     1081 ccctattttc agatcagtta tttcgcatta atatccagca gacccgctgc aaccgctgga
     1141 gccatgccgg gcgctaacgg gatacgcggg atcaccaggc aatgcagcag gtgataaata
     1201 tcctgtttgc cgtcccagac tttggtgatg accttattgt ccgcatccag ctcctgccac
     1261 caggaaccat tttcatagtc catcaggtac ttaatgcagt actcccacca tgtttgatac
     1321 caggtttcat actggcgatc gccagtgacg gtgtagagcg cgtaggccgt acccattgct
     1381 tcgacgatag gccaacgtac acgttcgcgg accaccggtt ttccttccca gtcaacggta
     1441 taaacaatcc cgtccgctcc atcgggtgcc caggcatcgc gcacggtggc gttaaacaga
     1501 cctttggcat cttctagcag ccatgctggt ggttgttcgc aacgggcttc cagggcagca
     1561 tggatgtgca gcattaaacg gccccattcg atccagtggc ctggtgtacc tccgaacgcg
     1621 cggaagcgat gcgccgggtt atctttgttg taatccggca gcggattcca ctgggtatcg
     1681 aagtgttcgt taacgcgata atgattattt ctggcgacgt cgtggataat cacggaagcc
     1741 acgcgaatcg cgcgatccag ccattttttg tcgtgagtga cgtcataaac aatcaagaaa
     1801 gcttccaccg cgtgcatatt ggcattgccg ccgcggtact cttcggtttt gctgaaggct
     1861 tcgtcccagg actccaggca catctgctct tcttcgctcc agaaatattt ctcgataatc
     1921 tcaatggtgt aatcgagcag cttgcgcgct tccgggtgac ccgttgtgac ggcgctggcg
     1981 gcacccagca gagcaaagaa gtgttgatag ccctgtttgg aggcatccac cacgccctcg
     2041 tcattcacgc aggcatacca gccgccatat tttttatcac gcagtgcgcc gttcatggct
     2101 ttaataccgt gatcaaccaa cgcgtaagcg ccaggtcgac ccatcgccgc agcaacggaa
     2161 taaacgtgca acatacgagc tgtgatccac agatgggtgc ccatctcttc tttgatttgc
     2221 cctttattgc ctaaccagcc aaaaccagtc ggcactacgg aatttttacc aaaatcaaag
     2281 atgcggtcgg tttcctgttc cagccaacgg ttgtggctta gggtgttaaa ccatttcatt
     2341 ccttttatcc tcatcttagc ggcgtttagc catcatttcg tcgacgatat cgccaagttg
     2401 ttgtaatttc ggtgcgcaaa cgtcacggag catcagctcg ttgtctggta aaccgacgac
     2461 cgatgcccag actgcacggc ctgccaggaa tcccgatgcg cccgccgtca ttgccacgcg
     2521 tacggcacgc gggaacagtt tttcgtcgac actggaagag aggatcaccc atggcatatt
     2581 gatatggtca ttcagacgtt gtgaagcaca gagaagctct tgttgcggac ctttgccata
     2641 aagcggcatt tcaactttgt agaggtcagc gccactgtcg cccagctctt tggcggcatc
     2701 gatgatcgct tgttcgcgat cgaatttatc gccacgacgc ggtgggcgga cgactggctc
     2761 aatgatgctt accagaccgt gtgagtggca cagttcgttg aactctttca ccatatccag
     2821 acgttgctgc gcatcttcat cactacgcta cagcaccagc agttttaagg ctttgccacc
     2881 gtcttgtttg atctgtagcg gattgatttt gcggtcaata accacgctat cgaccggaat
     2941 accgttgcca ggaatgaact catcggcggc gacaatcatg gcgcaacttt tggcaatcgc
     3001 gttttgctca accacctggc ggtagcagaa ttgttgatct accagaatcg ccgaggcata
     3061 aggcgagagg gcctttgcag cgttaacttt gaaatcagtt aaaacgctat cggctaccgg
     3121 agcgggtgcc ccagccgcgg caaacatcat gcgcatggct tcgcgctgat cgaccgccag
     3181 catggcaaaa ccgcccgatg cgcgcgtaat gtcgttgatg gtgtacttat tcattcattt
     3241 attcctttat ccagtcgtta catttttact ttggcagtca tcccggcact gacacggacc
     3301 tgttccagaa tggcggacca gtcctggcga ccgcgacccg ctgcgcgcgc ctggctataa
     3361 acctcccgtg aggcggcccc cagcggcatt ggcacatgca gctggttggc gacatcaagg
     3421 gcgatgccaa gatccttatg ggcaagatcg atcatgaagg cgggagaaag atctccgctg
     3481 aggactttgt ttggccagga agtggtgaag tgacctttac cggcggcggt accgctcatc
     3541 actttgacgg caacatcgaa gggaagattc agggcttcgc acaaaacggc ggcttctgcc
     3601 gaaagcgcat tgagcgcgat gctcatgtag ttgttgatga gcttaacgcg gatccccatg
     3661 cccggaccgc cagcgttgat caactcactg cccatcgcca tcaggatcgg tgtggcacgt
     3721 tcaacttgtt cagcggtgcc gccagccagc agtaacagag taccggtaat ggcatttgca
     3781 gaagtacggc ctaccggaac atccatcatg ctgaagcctt tggcttgcat atcggcaatc
     3841 aatttatcgg tttgcagcgg atggatggtg gacatatcaa tgaccagcgc atcggtagat
     3901 aagccttcgc aaacgccgtt ttcaccgaac aacacgttgc gcaccagatc gccattcggc
     3961 aacatggtaa tgataaattc ggcatcttta gcggcctgcg ccgggttggc ggcgggagtc
     4021 gcacctttgt ctaccagatg ccgcacagct tcggcgttca catcaaagac gcgaagttga
     4081 tgcccttgct gcaataaatt gctcgccatt ggcgaaccca tttgtcctaa accgataaac
     4141 gcgattgctg ccataaccct ctcctgaata cagtgatgtc actttttgtc atttatgaca
     4201 tgctttgctt gtctgttttt gatcgaattt gtaatttatc gtcaaaaaat tgacagccgt
     4261 cactttttaa acaattggtg aaattaaaat gaacgcatcc caaaatgttt aaggaatgac
     4321 catgattcgt gttgcttgtg taggtataac cgtgatggat cgcatctatt acgtggaagg
     4381 gttaccgacg gagagcggta aatacgtggc gagaaattat acggaagttg gtggcgggcc
     4441 agcggcgacg gcagcggttg cggcggcaag gctgggggcg caggtcgatt ttattggtcg
     4501 cgtaggtgat gacgacgcgg gcaacagcct gctggcagag ctggaatcct ggggcgttaa
     4561 cacccgttac accaaacggt ataaccaggc gaaatcttcg caatccgcca tcatggtgga
     4621 taccaaaggc gagcggataa tcattaacta ccccagcccg gatctgctgc ctgacgcaga
     4681 gtggttggag gaaattgatt tctctcagtg ggatgttgtg ctggcagatg tacgctggca
     4741 cgacggcgct aaaaaagcct tcaccctggc ccgtcaggcg ggtgtgatga ccgttctgga
     4801 cggggacatt acgccgcagg atatcagtga gctggtggca ttaagcgatc acgcggcctt
     4861 ttcagaaccg ggtctggcgc gcttaacggg cgtgaaagag atggccagtg cgctaaaaca
     4921 ggcacaaacg ctcacaaatg gtcatgtcta tgtgacccag ggtagcgcag gctgcgactg
     4981 gctggaaaat ggtgggcgtc agcatcaacc ggccttcaaa gttgatgtgg tagataccac
     5041 aggtgcggga gatgtttttc acggcgcttt ggcggtggcg ctggcaacaa gtggggattt
     5101 agcggagtca gtccgcttcg ccagcggtgt agcggcgtta aaatgcacac gtcccggtgg
     5161 acgagccggg atccctgact gtgatcaaac ccgatctttt ttgtcacttt ttgtataaaa
     5221 tgccagggtg atggtttttc gaggaatttt catgagcctt accgaactaa ccggtaaccc
     5281 gcggcacgac caactcctca tgctgatcgc cgagcgtggg tatatgaata ttgatgagct
     5341 ggcaaatctg ctggatgtct ccacgcagac ggtccgccgg gatattcgta aattaagcga
     5401 gcaaggcctg attacgcgcc atcacggtgg cgcgggtcgg gcttccagcg tcgttaatac
     5461 ggcgttcgag cagcgtgagg tttcgcaaac cgagaaaaaa aaagcgattg ccgaagcggt
     5521 ggcagactat attcctgatg gttcaacaat atttatcacc attggtacga ctgttgagca
     5581 tgttgcccgg gcgttactta accataatca tttgcggata atcaccaaca gcctgcgtgt
     5641 ggcgcatatt ctttaccaca acccgcgctt tgaagtgatg gtgcccggcg gtacgttgcg
     5701 ctctcataat agcgggatca ttggcccttc agcggcgtcc tttgtggctg attttcgtgc
     5761 tgattatctg gtaacaagcg ttggggcgat tgagagcgat ggcgcgttga tggagtttga
     5821 tgtaaacgaa gctaacgtgg tgaaaacgat gatggcgcac gcgagaaata ttctgctggt
     5881 cgccgatcac actaagtatc atgcttcggc ggcggttgaa attggtaacg tggcacaggt
     5941 cactgcgctc tttaccgacg agctgccgcc cgctgcgcta aaatcacgct tacaagacag
     6001 ccaaattgaa atcattcttc cccaggaaga cgcgtagatt tttgtgaccg ttaatctggc
     6061 ttcatacctt gccacatagc caaacccatc ctttcccgct acagttaatt tcttgtggcg
     6121 cgaaaggagg caaaaatgct ctatatcttt gatttaggta atgtgattgt cgatatcgac
     6181 tttaaccgtg tgctgggagc ctggagcgat ttaacgcgta ttccgctggc atcgcttaag
     6241 aagagttttc atatggggga ggcgtttcat cagcatgagc gtggggaaat tagcgacgaa
     6301 gcgttcgcag aggcgctgtg tcatgagatg gctctaccgc taagctacga gcagttctct
     6361 cacggctggc aggcggtgtt tgttgcgctg cgcccggaag tgatcgccat catgcataaa
     6421 ctgcgtgagc aggggcatcg cgtggtggtg ctttccaata ccaaccgcct gcataccacc
     6481 ttctggccgg aagaataccc ggaaattcgt gatgctgctg accatatcta tctgtcgcaa
     6541 gatctgggga tgcgcaaacc tgaagcacga atttaccagc atgttttgca ggcggaaggt
     6601 ttttcaccca gcgatacggt ctttttcgac gataacgccg ataatataga aggagctaat
     6661 cagctaggca ttaccagtat tctggtgaaa gataaaacca ccatcccgga ctatttcgcg
     6721 aaggtgttat gctaaaaacc attcaggaca aagccaggca ccgtacccgt ccactatggg
     6781 cctggctaaa actactctgg caacgtattg atgaggacaa catgacaacc ctggcaggta
     6841 accttgccta tgtgtcgttg ctctcattag tgccgctggt tgccgttgtt tttgcgcttt
     6901 tcgccgcttt tcccatgttt tccgacgtca gcattcagtt acgtcacttt atttttgcca
     6961 actttctgcc tgctactggc gatgttattc agcggtatat cgaacaattt gttgctaatt
     7021 ccaacaagat gaccgccgtt ggggcgtgcg ggctgatcgt caccgcgtta ttgttgatgt
     7081 actccatcga tagcgcgttg aataccatct ggcgcagtaa acgagcgcga ccaaaaattt
     7141 actcgttcgc cgtgtactgg atgattttaa cgctggggcc gctgctggca ggggccagtc
     7201 tggcgatcag ttcctatttg ctctccctgc gctgggcgag cgatctcaat actgtcatcg
     7261 acaacgtgct gcgtattttt ccgttgctgt tgtcgtggat ctccttctgg ttgctgtaca
     7321 gcattgttcc taccatccgc gtacctaacc gcgacgcgat tgtcggcgcg tttgtcgccg
     7381 cactcctgtt cgaagcagga aagaaaggtt tcgcgcttta tatcaccatg ttcccgtcat
     7441 atcagctcat ttacggtgtg ctggcggtga tccccattct ctttgtttgg gtctactgga
     7501 catggtgtat cgtcttgctt ggcgcggaaa ttactgtcac tctcggggaa taccgcaaac
     7561 taaaacaagc agctgaacaa gaagaagacg acgaaccatg attgcattaa ttcaacgcgt
     7621 aacccgtgcc agcgtcaccg tggagggaga agtgacgggc gaaattggcg cgggactttt
     7681 ggtgttattg ggtgtcgaaa aggatgacga cgaacagaaa gcaaaccgtc tgtgcgagcg
     7741 tgtgctcggc taccgcatct ttagcgatgc cgaaggcaag atgaatctca acgtgcaaca
     7801 ggcgggcggc agtgtactgg tggtttccca gtttaccctc gccgcagata ccgaacgggg
     7861 gatgcgccca agtttctcca aaggcgcatc accggatcgc gcagaggcgt tatatgacta
     7921 tttcgtcgaa cgctgccgcc agcaagagat gaacacgcaa acaggacgct tcgctgccga
     7981 tatgcaggta tcgctggtca atgatggccc cgtgacattc tggttgcagg tatgagccag
     8041 cttccagggt tgtcacggga aacaagagag agtatcgcta tgtatcacct tcaggttcca
     8101 caaacagaag aagagttaga gcgttactat cactttcgct gggaaatgtt gcgtaagccc
     8161 ctgcatcaac caaaaggttc ggaacgcgac gcgtgggatg cgatggcgca tcaccagatg
     8221 gtcgtcgacg agcagggtaa tctggtggcg gtaggccgat tgtatattaa tgccgacaat
     8281 gaagcgtcca ttcgctttat ggtcgttcac ccggacgtgc aggacaaagg gttaggaacg
     8341 ctgatggcga tgaccctgga gtcggtggcg cgtcaggaag gcgttaagcg cgtgacctgt
     8401 agcgcccgtg aagacgcggt ggagtttttc gccaagctgg ggtttgttaa tcagggagaa
     8461 atcaccacgc caaccaccac gccgattcgc cattttttga tgattaagcc cgtcgccact
     8521 ctggatgata ttttgcatcg cggcgactgg tgcgcgcagc tgcaacaggc gtggtacgaa
     8581 catatcccgc ttagtgaaaa aatgggcgtg cgtattcaac aatataccgg gcaaaaattt
     8641 atcaccacca tgccggaaac cggcaatcag aatccgcacc atacgctgtt tgccgggagt
     8701 ttattctcgc tggcgacgct caccggttgg gggcttatct ggctgatgct gcgtgaacgc
     8761 cacctcggcg gaacgattat tctggcggat gcgcatatcc gctacagcaa accgattagc
     8821 ggtaaacccc atgcggtagc cgaccttggt gccttaagcg gcgatctcga ccgtctggcg
     8881 cgcggacgaa aagcacgggt gcagatgcag gtcgaaatct ttggcgacga gacgccgggt
     8941 gcagtgtttg aaggcacgta tatcgttctg cccgcgaagc catttggccc gtatgaagag
     9001 ggcgggaacg aagaagagta gcttatggag aggggcggtg ctgcctctct cattcattcc
     9061 ggtcatttac atcagtccct gactattccg gttatattga ataagaactt tataaatttt
     9121 tattcttcgt tggcaccata cgtgtcttgc tacatcactg ttcaggagca tttaaatatt
     9181 atttgtagag ctgagtggca attattgcag ataatgctta actcatcata ttagttcagg
     9241 tttgagcgat ttttgatagt caaaaccccc tctggtgcca tacttttttc ataaatattt
     9301 ttaaaatcta tagctcacaa atattatgca aagataatgg gctggcatac attgtcagta
     9361 tcattcattt actgctctgt atgttatgaa tcattatgtt gtatgtttgc tcttaaaccg
     9421 tgggaaggag gctataatgg ttgccaatat taatctcatt aaacaagaaa gttattctgt
     9481 tgttaattta gaaaaacaac taatgagtaa taaatcaccg aagcaaacgc tattaaatc
//
LOCUS       DAZSNO010000149         9492 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZSNO010000149 DAZSNO010000000
VERSION     DAZSNO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9492
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.149"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..189)
                     /locus_tag="ACHWN2_003336"
     CDS             complement(<1..189)
                     /locus_tag="ACHWN2_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR8932747.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            709..1404
                     /gene="minC"
                     /locus_tag="ACHWN2_003337"
     CDS             709..1404
                     /gene="minC"
                     /locus_tag="ACHWN2_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR8932748.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1428..2240
                     /gene="minD"
                     /locus_tag="ACHWN2_003338"
     CDS             1428..2240
                     /gene="minD"
                     /locus_tag="ACHWN2_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR8932749.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2244..2510
                     /gene="minE"
                     /locus_tag="ACHWN2_003339"
     CDS             2244..2510
                     /gene="minE"
                     /locus_tag="ACHWN2_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR8932750.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2882..3226)
                     /locus_tag="ACHWN2_003340"
                     /pseudo
     CDS             complement(2882..3226)
                     /locus_tag="ACHWN2_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3261..3380)
                     /locus_tag="ACHWN2_003341"
     CDS             complement(3261..3380)
                     /locus_tag="ACHWN2_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR8932751.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3341..3526)
                     /gene="ymgJ"
                     /locus_tag="ACHWN2_003342"
     CDS             complement(3341..3526)
                     /gene="ymgJ"
                     /locus_tag="ACHWN2_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR8932752.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3627..3800
                     /gene="ymgI"
                     /locus_tag="ACHWN2_003343"
     CDS             3627..3800
                     /gene="ymgI"
                     /locus_tag="ACHWN2_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR8932753.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3802..4146
                     /locus_tag="ACHWN2_003344"
     CDS             3802..4146
                     /locus_tag="ACHWN2_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR8932754.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4150..4485
                     /locus_tag="ACHWN2_003345"
     CDS             4150..4485
                     /locus_tag="ACHWN2_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR8932755.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4542..6863)
                     /locus_tag="ACHWN2_003346"
     CDS             complement(4542..6863)
                     /locus_tag="ACHWN2_003346"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR8932756.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6980..7189)
                     /locus_tag="ACHWN2_003347"
     CDS             complement(6980..7189)
                     /locus_tag="ACHWN2_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932757.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7589..7808)
                     /gene="ymgF"
                     /locus_tag="ACHWN2_003348"
                     /pseudo
     CDS             complement(7589..7808)
                     /gene="ymgF"
                     /locus_tag="ACHWN2_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
     gene            complement(7940..9463)
                     /locus_tag="ACHWN2_003349"
     CDS             complement(7940..9463)
                     /locus_tag="ACHWN2_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR8932758.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2659 a         2204 c         2079 g         2550 t
ORIGIN      
        1 ggctttttcg ccgagatatt tccttgttaa ctctggggaa atgccatctg agttcacaca
       61 aatatacggg tcgcaaacaa cgccgcacgt cggagattga agtccgccag cgtcatgtcc
      121 agacgccatt gatgagaaca tcatccccac acctgacaat ccgatataaa taatacgcat
      181 catattcata gcacatcctg ataatccaac cgtacaacac ttatgctcat ttcgtcatca
      241 atccgtaatc aaaaccgctg acctggacga gttacgatga cctgacagaa gtatcagacg
      301 tcttctgcgg tttctatgat agagcaattc tgtggcggat attaagataa cgggtatctg
      361 ttctttaatg atgctgcaaa aaatggaaag ttaagaaagc ctggcgtggt gaaaaaatac
      421 acttgcctca atactggata tttaaacagg tctggagatg ctgtcacgat tagggtgcgg
      481 aaaaaggaag cgtaaataag aagaatcaaa agtacgacgg caatgggttg attgacaagg
      541 gtatttttta agctatgaat cagcgccatt tatcacagaa tagactttta ctctgaataa
      601 atgggagggt gacttgcctc aatataatcc agactataac atgccttata gtcttcggaa
      661 catcatcgcg cgctggcgat gattaatagc taattgagta aggccaggat gtcaaacact
      721 ccaatcgagc ttaaaggcag tagcttcact ttatctgtgg ttcatctgca tgaggcagaa
      781 cctaaggtta tccatcaggc gctgaaagac aaaatcgctc aggcccccac atttttaaaa
      841 catgcccccg ttgtactcaa cgtcagtgca ctggaagacc cggtaaactg gtcagcgatg
      901 cataaggcgg tttcggcaac cggtttgcgg gttattggcg taagtggctg caaagatgcg
      961 caacttaaag ccgaaattga aaaaatgggg ctgcctatcc tgacggaagg aaaagaaaaa
     1021 gcgccacgtc cagctcccgc accgcaggct ccagcgcaaa atacaatgcc ggtcacaaaa
     1081 acgcgtttaa tagatacccc ggtgcgttcc ggtcagcgta tttatgctcc acaatgtgat
     1141 ctgattgtta caagccacgt tagcgctggg gccgaattga ttgccgatgg gaacattcat
     1201 gtctatggca tgatgcgcgg tcgtgcgctg gcaggggcaa gtggtgaccg ggaaacgcaa
     1261 atattttgta cgaacctgat ggcggaattg gtgtccatcg caggtgaata ctggctgagt
     1321 gatcaaatcc cagcagaatt ttatggcaaa gcggcgcgac tgcagttagt cgaaaacgct
     1381 ttgaccgttc aaccgttaaa ttgatccctt tttaacaagg aatttctatg gcacgcatta
     1441 ttgttgttac ttcgggtaaa gggggtgttg gtaagacaac ctccagcgcg gccatcgcca
     1501 ctggtttggc ccagaaggga aagaaaactg tcgtgataga ttttgatatc ggcctgcgta
     1561 atctcgacct gattatgggt tgcgaacgcc gggtcgttta cgatttcgtc aacgtcattc
     1621 agggcgatgc aacgctaaat caggcgttaa ttaaagataa gcgtactgaa aatctctata
     1681 ttcttccggc atcgcaaaca cgcgataaag atgccctcac ccgtgaaggg gtcgccaaag
     1741 ttcttgatga tctgaaagcg atggattttg aatttatcgt ttgtgactcc ccggcaggga
     1801 ttgaaaccgg tgcgttaatg gcactctatt ttgcagacga agccattatt accaccaacc
     1861 cggaagtctc ctcagtacgc gactctgacc gtattttagg cattctggcg tcaaaatcac
     1921 gccgcgcaga aaatggcgaa gagcctatta aagagcacct gctgttaacg cgctataacc
     1981 caggccgcgt aagcagaggt gacatgctga gcatggaaga tgtgctggag atcctgcgca
     2041 tcaaactcgt cggcgtgatc ccagaggatc aatcagtatt gcgcgcctct aaccagggtg
     2101 aaccggtcat tctcgacatt aacgccgatg cgggtaaagc ctacgcagat accgtagaac
     2161 gtctgttggg agaagaacgt cctttccgct tcattgaaga agagaagaaa ggcttcctca
     2221 aacgcttgtt cggaggataa gttatggcat tactcgattt ctttctctcg cggaagaaga
     2281 acacagccaa cattgcaaaa gaacggctgc agattattgt tgctgaacgc cgtcgcagcg
     2341 atgcagaacc gcattatctg ccgcagttgc gtaaagatat tcttgaggtc atttgtaaat
     2401 acgtacaaat tgatcctgag atggtaaccg tacagcttga gcaaaaagat ggcgatattt
     2461 ctattcttga gctgaacgtg accttaccgg aagcagaaga gctgaaataa gcccgctgta
     2521 aaaacgcatt tatcttcaag gcagagtcat ttctgccttg agtttttcat ccctctcatc
     2581 cacgttgtgg taaagcggcg agtattcttg ctgatactcc tcattgctat ttccacctcc
     2641 ccctttacac cttaaggctg ttcccacgca cagaacaaaa tgtttatgcc tggttgagta
     2701 aataaccatg ttattagtgt gtatatactg ggagatgata accccgcaat ttcattattc
     2761 attgttctcc cataggatga ggtttcccgc cgtaactggc gggctttttt tgccccagaa
     2821 ttttctcttt caacatcctg taaacgaaaa ctgcgccgaa gcgcagttta atcgttgatg
     2881 gtcagaacga ccagttcaca ccagccaccg cgttccacgg ggactccaca ccggcaccat
     2941 ggctataccc caccccgaaa tgcccgctta acgtactgct gaatgaggct ttaatacctg
     3001 cctggtatat tccacgtctg cccgacaaat cattgacaaa attaccgtca ctattcactt
     3061 tcacccgatt atcatcgaca aattctttgc gcacagccgc cttcaggcag ggttcaattt
     3121 ccataccgtt ccccagacgc atgttgtaac tcagcgttgc acccagttca cgatatatac
     3181 tgcgggtatc gactgatttc gattccatgc cattggataa atgatagtag cgtctaagtg
     3241 acctaagctc tgctgcaaca tcagcccgca ggcaccagac caggggattc atcctgaaga
     3301 gacagcgcag gtgtattgtg ttcaccgctc accaaagaca tcatgatgaa atgatgatat
     3361 cccgcataag aaagaggcat tttttacacg cagtgcgctg aagtgtagtt ggataaaaaa
     3421 tcactctttt cagataatac tgtcgtacgc ttatctttag acaatgaggc cgtcggaaaa
     3481 ataaaatgac taatgtaatc gttgctgatg aatatcttct gctcacataa aaatcacaca
     3541 ataatattga taaatcagat tatcttacct ttacaacatt gcgttaacag tcaatgacac
     3601 ttcttgaata aaaatattgt caatcaatga gttattcctc atttaagatt attctcattc
     3661 acgtaaaaaa cataattcct atcatcacag ccacgctcat tttgaattat ctgaataata
     3721 gcgagcgttc tttagttaag cagatcttaa ttgaagacga aattattgtg tgtgcaactt
     3781 acttaatacc ggacatctaa gttgaagaaa aaaatacttg ccttcgggct tatctcagca
     3841 ttgttctgct ccacgccagc gatggcggat atgaaccgca ccacgaaagg tgcattgcta
     3901 ggcgcaggcg ttggtttact tactggcaat ggtgttaatg gcgtactgaa aggtgcagct
     3961 gttggcgctg gtgttagtgc agtaacagaa aaaggccgcg acggtaaaaa tgcgcgtaaa
     4021 ggtgcaaaag tgggtgccgc cgttggtgct gtcactggcg ttctgaccgg caatggcctc
     4081 gaaggggcga ttaaaggtgc cgtgattggc ggtacgggtg gtgcaattct gggtaaaatg
     4141 aaataaggaa tgatgatgaa aaaatttgct ttactggcgg gcctgtttgt tttcgcacca
     4201 atgacctggg cgcaggacta caatattaaa aacggtttgc cgtcagaaac ctatattact
     4261 tgcgcagaag ccaacgaaat ggcaaaaact gacagcgcac aggttgcaga aattgttgcg
     4321 gtaatgggta atgccagcgt tgccagccgt gatttaaaaa ttgagcaatc tccggaactg
     4381 agcgccaaag tggtagaaaa actgaatcag gtttgcgcta aagacccgca aatgctgttg
     4441 atcacggcaa ttgatgacac catgcgtgct atcggcaaaa aataatattt catatgtacc
     4501 gcgtcgatga atacgacgcg gttttttagc taacggcggg atcagaactt atatttcagg
     4561 cccaccatca ctgcggtgtc attgtagcca ttatcaccca gctgcacgcc cacattcccc
     4621 cagacgctgg ccgctggatt caggttgcca tttacgccaa gtttaatttc ccccaaatta
     4681 ctcaccccat cttgttcgac tttcacaccg ttcattgaga cagcaaaatc tttactgtta
     4741 tggatccagt tcatttcaac aaaaggttca aagtcgtcac cgtgcggacc gctgtttttc
     4801 tcctgagtac gaataaaggt gcggaagccg agtcgggtct ggatgttgtt atttgcgtca
     4861 ttctcaatac gtgagccatt ggcttccgtg tgcgcattat gatcgacgcc catccagata
     4921 gcctgtgcct gtggctggaa gatccacgta tatttcgccg cttccagacc aaaggattta
     4981 ttcagggcaa aaccgtaacc cgcttccagc gagacggttg cacctttcgc gctataggat
     5041 tcataggcta attcatcccc tttcacggag tttttaaacc agctatattg cgcccaggag
     5101 tcaatgtatg cgcctttttt actgatgtca tcggcaaacc aagtggcata cagccctgcg
     5161 ctatagcctc tgacactccc tttcgaacga taatccgaca cgctggaatg agttaagttg
     5221 tagtcgcggg cataaccagc catcactccc aaacgccaac tgtcactatc ggtaaaacca
     5281 ccggttaaca gatcgccccc taactgcgag acgtagcgat gcgaggtcgt tctcaactgt
     5341 ccgttgctgt cacgccaggc attatgcccg ccaatttgac gtagccaaag tcggcttgaa
     5401 cgctcctgtt cagttacagg atcgatgtag cgcgtttcac ccgcacggtc gttcagacgc
     5461 ataacaaaaa gagtgttagc ggctgccagg ttggcgacgt aacttccagc ttcggggcgc
     5521 aggacggggg taggcgcaac agtaggcgct gaacttcctc cggagtttgt ttcaccgccg
     5581 ctcgttgcct ggaagttagt cagataccag tttttattgg tcgcttcggt attacctcgg
     5641 gttaatgaat attcgtaggc accggcgaaa atcctcgaat ccttaataaa ttccccattt
     5701 gattccccct caacgctgat aatctctatc ccatttaacg tgtaagctcc gctgcctcct
     5761 gcattggtaa ctttaactcg ggttgttcca gaggtatttc cattaataac gactttatcc
     5821 gtagctgaat tatcatcatc caattcagtt ctgagatgca atacgccatt attaccaacg
     5881 taattttcag ttattgttaa tcttgttggt tcgacatctc gtccatctgc ccgggaaata
     5941 tagaccgtac tattatcaac cgtgaggttg ctcaccgttg agtcagcttt aatatcccag
     6001 gtactattat ctgaaagcga cagataggta tgagtgttat catctgttga aatattagct
     6061 gaacccgtta tttttgaatt agttgcatcg acggtcaaat gacctttaat attattgata
     6121 aaaacatctg cagccgatga tacctcacta tccgtaatgg tcacgttact cgtctcatct
     6181 ttatggtaga ggatttcatt tgctgaccgt agtgagctac cagataaaat atctaccgtt
     6241 gcttcctgac catttatgga agactccagc acaattccag acccctgatg cgcgactgca
     6301 gatgtatttt tcagcgtcac cgtgccgcca ttataaattt tagcaatcgc ggcgtcagga
     6361 cccgttgtat aaatttctcc ctggttaaat gaaactgtcc cctggcgggc agcgatggca
     6421 tgagcattgt ccccatgcgt ggtgatatca acatcggtta atgtaacgct ggcattggta
     6481 taatcaagcg tgtcaccagc cttgacgaca gtgccattag cgtacgcggc ataactttct
     6541 gttccgtggg ttgtgatctc cccttttgcc atatccagtt ttgctatggc attattttca
     6601 ttacttcctg tatcggcagt actttcttca tcaagagaag atacacacgc acacgcgctt
     6661 ccgggagatg cacacgcaag aacgccgact gcgtgattac ccgcagtaac aataaatgta
     6721 tcatctccgt cagaaaaaga gggggtaacc gaaccgtcag agttgactct tattatggtg
     6781 tcagtattca ggtttccact cgtttgactt agcaaaccaa ttgagtaatc ccccgttgtg
     6841 acaatcgtgt ttttaccctg caaaataaca gcaccgccaa attcacttac gattgcgcta
     6901 gctgttaatg ctgggcttag aggactattg tcagaaggat ataaaccgcc cgttgttaca
     6961 atgacgccat tgttaatggt taacttacta ccctctcctg caacggatac ccgtaacttc
     7021 cattacccgt tgttataact agcgcattat caatatcttt tacttttcca tcttttattg
     7081 ccacagcatg tttatattca tcgtgctgaa taaactgttc agcatatgca ttacccaccg
     7141 ccagagcaat aagtcccaaa ctaaacccgg ggagtttttt cagtttcata gattaactat
     7201 ccattcattg gattaacaaa cattcctggt caaataacct atgagctcag attaccaaaa
     7261 cggagggtaa agacaaaaac aaaagcacaa aaaatttaaa tagataaaca gcgacaatat
     7321 ataaatgata aataagtcgg cattatcatt tttcacctta ttttcatgac attgatcact
     7381 ttggggtgac tattttgttt aacaaataac tcttcgacat caaaattatt aatagttgcc
     7441 atggtaatta tttcggtgat atataaaaac aactgtgata acaccaaggt tgacaactat
     7501 ttgcgcaata attcctttcc ttcaatgatc caggcgttca ctgccagcaa cccattcagc
     7561 cgcatactga cagtgagaaa ctgaaagatt attgctgatc cctgtggcta acgagtcttt
     7621 ctcttaaatc gattaaccag attgatcgcc agccaaacaa gggcattaag caaatttatc
     7681 gtcacaagca cttctcccag cccggtactg ggttttgcaa taaggatgac caggagggta
     7741 atcagcataa atgcaatgga aaagatgata tagagagtga aataatccag attattacta
     7801 ttattcatgc tactaatggc aatcctgtca aaactttcaa acaattatag caaacaaaat
     7861 aatgaacaat agtaacaaga ggcaacgagc taattagctg tctataatta caccgcacta
     7921 tggccttact gatcaatttt cactcaacca caaccttcac cttcggttta gaaaggataa
     7981 ttttgaccag gtcgatgtat gtaacaggtt tatagaaata ataaccctgc tgaaatgtga
     8041 tattattttg gttcagatag ccaagttgtt ctttcgtttc gacaccttca gcgacgatac
     8101 tgatcgaaag tttacgcgcc agatccaata cacaatccag aattcgggtt gattctgggt
     8161 ctgcgtttac gcggccaacg aaactgtgat caattttgat ataatcaata tgcaggtcat
     8221 gaagataaga aagccctgag tagccagtac cgaaatcatc cagcgcgatg acaaaaccat
     8281 tttcatgcag aatgttcaac cgctgaacca gactttcatc aacattcaat ggctcacgct
     8341 cggtgacttc cagaacaagg tttaaatcgc ggcgggtaaa actgtcacgg aaatttaaac
     8401 actcgtcgac aaacgtcggc gaaataatat gcgaagcgct aaaattaatt ccaatatgaa
     8461 aaccttccgg cagtttactc gcgatagcgt tcatctgtct ggcaacctga ttcatcaggc
     8521 tttgtgtaag cggaacgatt aatccggatt tttcagcaag tggaataaat gccgcgggtg
     8581 aaatatatcc accgtgaggt tgtttccagc gggctaacac ctcaactccc cgcaatgtcc
     8641 cttcccgacc atttaccaca ggttggtaaa aagggacgat ctccccatta tttattgccc
     8701 gtcgcaggat ctcttcaggc gttgcgcttt tattgaaata tctatcaagc aggaaggcag
     8761 cggcgctggc gattaacaga ataaatatta aaatcccaaa gcctttatcg ataagtctgt
     8821 agaagctaaa gagtggcggc gggttaaaga taatagtgaa atgatatttt gtggagtgaa
     8881 ccttctcggt atagggataa tgtccattaa gcgtcatcac atcagccgtt ggtccaataa
     8941 tgtcatccgc cacgcgtaat acatacctta cccctttcaa gggtacatta agcgccccgc
     9001 gaatatgttg atcacttatc gtaaccaaaa tgcgcgtatc tgcaaattgg ctctgataga
     9061 gcaggatagg taatctatta acggtgtcga ttgccggagc caacagtaaa ttactgtccg
     9121 ggacaacagg aatacgagac agcaggaccc gattcccagg cagggatgta caccagacta
     9181 ttccctgttt gagaatgatg attgtgcgca ggtgaggttt cagagctgct tcagtgccaa
     9241 gctgatattg ctcctcgagg tcgcattcct tcccggcaat gttaaccgct gtttgagtcg
     9301 cctgtgacgc ttcttcaagt atatgattga tattgttggc ggcatatctt gctcccgcca
     9361 ggtactctgc ttttgcggaa taccagagct ggatgttcaa aatagcgaca ccggtaataa
     9421 acaagcatat cgcaacgagg atgggtatga gtgtattgcg catttgcttg tcccggccaa
     9481 agggtttaat ac
//
LOCUS       DAZSNO010000150         9462 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZSNO010000150 DAZSNO010000000
VERSION     DAZSNO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.150"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="ACHWN2_003350"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="ACHWN2_003350"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR8932759.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="ACHWN2_003351"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="ACHWN2_003351"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFR8932760.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="ACHWN2_003352"
     CDS             3115..3399
                     /locus_tag="ACHWN2_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR8932761.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHWN2_003353"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHWN2_003353"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR8932762.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHWN2_003354"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHWN2_003354"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR8932763.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHWN2_003355"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHWN2_003355"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR8932764.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="ACHWN2_003356"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="ACHWN2_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR8932765.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2271 a         2494 c         2392 g         2305 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgaccccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc gacttcgatc acatatcgat aaataatatt at
//
LOCUS       DAZSNO010000151         9356 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZSNO010000151 DAZSNO010000000
VERSION     DAZSNO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.151"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            193..1506
                     /gene="gltP"
                     /locus_tag="ACHWN2_003357"
     CDS             193..1506
                     /gene="gltP"
                     /locus_tag="ACHWN2_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR8932766.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(1584..2273)
                     /gene="yjcO"
                     /locus_tag="ACHWN2_003358"
     CDS             complement(1584..2273)
                     /gene="yjcO"
                     /locus_tag="ACHWN2_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR8932767.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(2367..4514)
                     /gene="fdhF"
                     /locus_tag="ACHWN2_003359"
                     /pseudo
     CDS             complement(2367..4514)
                     /gene="fdhF"
                     /locus_tag="ACHWN2_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(4712..6178)
                     /gene="mdtP"
                     /locus_tag="ACHWN2_003360"
     CDS             complement(4712..6178)
                     /gene="mdtP"
                     /locus_tag="ACHWN2_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR8932768.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(6175..8217)
                     /gene="mdtO"
                     /locus_tag="ACHWN2_003361"
     CDS             complement(6175..8217)
                     /gene="mdtO"
                     /locus_tag="ACHWN2_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR8932769.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            complement(8217..9248)
                     /gene="mdtN"
                     /locus_tag="ACHWN2_003362"
     CDS             complement(8217..9248)
                     /gene="mdtN"
                     /locus_tag="ACHWN2_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR8932770.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(9267..>9356)
                     /locus_tag="ACHWN2_003363"
     CDS             complement(9267..>9356)
                     /locus_tag="ACHWN2_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932771.1"
                     /translation="RANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         2079 a         2508 c         2516 g         2253 t
ORIGIN      
        1 ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg ttaaagggtt gcgcaacata
       61 ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta caaaccaacg caacacaatt
      121 catgccctgg cagtatgtca cgttctcgcg tttctgaacg gggaacggcg ctccattgag
      181 gaagttattc tgatgaaaaa tataaaattc agcctggcct ggcagattct gtttgctatg
      241 gtgctgggca ttctcctggg aagctacctg cactaccata gcgacagccg cgactggctg
      301 gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc atctgatcaa aatgattgtt
      361 gtgccgattg tgatctccac gctggtggtg ggtatcgcgg gtgttggtga tgccaaacag
      421 ctcgggcgta ttggcgcgaa aaccattatt tacttcgagg tgatcaccac cgtcgccatc
      481 attttgggga tcactctggc gaacgtcttc cagcccggtg ccggggtgga tatgtcgcag
      541 ctggcgaccg tcgatatctc gaaatatcag agcactacgg aagcggtaca aagcagttcc
      601 cacggcatta tgggcacgat tttgtcgctg gtgccgacga acattgtggc gtcgatggcg
      661 aaaggcgaaa tgctgccgat catctttttc tcggtgctgt ttggtctggg gctttcttcc
      721 ctgcccgcga cgcatcgtga accgctggtg accgtgttcc gctccatctc tgaaaccatg
      781 tttaaagtga ctcacatggt gatgcgttat gcaccggtgg gtgtgtttgc gctgattgcg
      841 gtgacggtgg ctaactttgg tttctcgtct ctgtggccac tggcgaaact ggtgctgctg
      901 gtgcatttcg ccattctgtt cttcgcgctg gtagtgctgg gaattgtggc acgtctgtgc
      961 gggttaagcg tctggatcct gattcgtatt ctgaaagatg agctgattct ggcgtactcc
     1021 actgccagct ctgaaagcgt actgccgcga attattgaga agatggaagc ctacggagca
     1081 ccggcgtcga tcaccagctt cgtggtgccg accggttact cttttaacct tgatggttcg
     1141 acgctgtatc aaagtattgc cgctattttc atcgctcagc tgtatggcat tgacctgtcc
     1201 atctggcagg aaatcattct ggtgctgacg ctgatggtga cctcgaaagg gattgctggc
     1261 gtggctggcg tgtcgtttgt ggtgttgctg gcaacgctgg gtagcgtagg tattccgctg
     1321 gaaggtctgg cgtttattgc tggtgttgac cgtatcctcg acatggcgcg tactgcgctg
     1381 aacgtggtgg gtaatgcgct ggcggtgctg gtgattgcca agtgggaaca caaatttgac
     1441 cgcaaaaagg cgctggctta tgagcgtgaa gtgctgggca aatttgataa aactgcggat
     1501 caataattga agattgccgg ggatatcccc cggcaatgtg tggatgcctg atgcgacgct
     1561 tgccgcgtct tatcaggcct accttaaccg ttggttaatt tttcaaactc ttcacacccg
     1621 gtatcaaacc cttccataca gctcaggttc aaccagtgca gcgccttttg tttgttcttc
     1681 tcgataaaac cttcctcacc gtttaagaac atcattcccg cccagtactc ggaataaccg
     1741 gttcgggaaa ttgcagagct gcgtttgaaa taccaggttg ccttgtcatc gtcggcctta
     1801 atgcccacgc cgttggcgta aatcaaacca agcagcattt gggcatcgac ggcggagtca
     1861 ttctccagat cttccgaggc gttttctaac agcgaaatcg cctttggata atccggttta
     1921 cccgcttgag tatttaccag aatatgcgcc agcgttactt caccttcttt actgcccgct
     1981 tgcgccgctt tctctgcaag aacttttgct tgtggatagt ccagactgac cggattggtg
     2041 attttgatct gcgccaacag cgcgcaggca tcagcatcac cgctgtcggc ggctttctgt
     2101 gcccaatatt cggctttgct caaatcgccg gagctaaacc agctgtcggc aagaaaatat
     2161 tgtgcacgtc ggtccccggc ctctgctgcc tttaaatact ggctgccagg ctcggagtcg
     2221 ttcgcgtggg caaagaatgt caaaaacaac atcaatgcga taattttttt cattttgata
     2281 atacgattgg gtagggagta accagtataa agagttcgaa tggataaaaa aacagcctcc
     2341 gaaaggaggc tgtagaaagg actgtattac gccagtgccg cttcgcgcag gcgagttttc
     2401 aacttgttgt actcgtcaat cacgtactgc tcggcggcgc gctgatcgga gatcggctcg
     2461 acgcgcacgg cgcagtattt gtattccggc gttttcgtaa tcgggcttaa gctttcggta
     2521 accagctcgt tacaggcacc aatccaccac tggtaggtca tgtaaatcgc ccctttgttc
     2581 ggacgatcgc tgacctgcgc acgggtgata attttgcctt tacgcgagtg cactcaaacc
     2641 aatgcctcat cttcaatacc cagacgtttg gcgtcttcgg tattgatttg tgcgtagcca
     2701 ggttcatcag ccagcgccgc cagtgccgca cagttaccgg tcatcgaacg gcaagagtag
     2761 tgaccaactt cacgcaccgt tgacagtacc atcgggtact cgtcggtgag tttgtcgatt
     2821 ggcgctaccc agtcgcaggt gaagaactgc gccagaccgt tcggggtatc aaacttctct
     2881 ttaaacagat aagaagtccc ctgatcggca tctgaagtat cgcggcaagg ccactgaatg
     2941 aagcccagtt cgcccatttt ctcgtaagtc gcaccgtaga aatccgggca cagatgacgc
     3001 aactcatccc agatctcctg ggtgttgttg tagtgcatcg gataacccat acgggtggcg
     3061 atttcactga tgatttgcca gtccgttttc agatcccatt tcggttcaac tgctttgaag
     3121 aaacgctgga agccacggtc agccgcagta aacacgcctt catgctcgcc ccacgacgtt
     3181 gacggcaaaa tgacatccgc cgccgacgcg gttttggtta taaagatgtc ctgaacgatg
     3241 accagttcca gatcttcaaa ggctttacgt actgccgaca gctccgcatc agtttgcagc
     3301 ggatcttcgc ccataatgta cgcggcacgc acttcgccat gcgctgcgcg gtgcggcagc
     3361 tcgctgatgc gatagccggt atgagcaggc aggctttcca cgccccaggc tttggcgaat
     3421 ttttcgcggt tagccggatc tttcacgtac tggtatcccg gatacgtatc cggcagcgcg
     3481 cccatatcgc aggcaccctg cacgttgttc tgaccacgaa ccgggtttac acccgcatgc
     3541 ggcttaccga ggttactggt cagcatcgcg aggctggtca gagaacgcac ggtttccacg
     3601 ccctggtaga actgggttac acccatgccc cacaggatgg cggcgctttt cgcctgggca
     3661 tacatccgtg ccgcctgacg aatctcactg gcgctgacac cggtgatatc ttcaaccgac
     3721 tccggcgtgt agccttcaac gattttacga tactcttcaa agccttctgt acgggaagcg
     3781 acgaacgctt tgtcgtacag attttcttca ataatgacat ggcccatcgc attcaacagc
     3841 gcgatgttcg agccgttttt cagcgcaatg tgcatgtcag caatgcgcgc ggtttcaatt
     3901 ttgcgcggat cgcagacgat aattttcgcc ccgttacgtt tagcgttaat tacgtgattc
     3961 gccacgattg ggtgggaatc cgccgggttg tacccgaaaa cgaacactaa atcggtatta
     4021 tcaatttcgt taatagcatt gctcattgcg ccattaccga ccgattggtg cagacctgca
     4081 accgatgggc cgtgtcagac acgagcgcag cagtcaacgt tattggtacc aataacggcg
     4141 cgcgcaaatt tttgcattac atagttggtt tcgttacccg taccacgcga ggagccggtc
     4201 gtctggatgg catccggacc gtacttctct ttgatggcgc tcaggcgctc ggcaacgtaa
     4261 ttcagtgcct catctcagga aacaggttcg agtttgccgc cacgctggcg acggatcatg
     4321 ggggttttca ggcgcggggt caggatctgg gtatcgttaa tgaaatccca gccataataa
     4381 cccttcagac acagggtacc ctggttggtt ttcccctgcg ctgcctccgc ccggacgatt
     4441 ttgccgttat cgacgaccag gttgattttg caacctgatg cgcaataggg gcaaaccgtg
     4501 acgacttttt tcatcggtct cgctccagtt aatcaaatca cgcatacgcg ctctcgacta
     4561 cagtatgcat cttttatgcc acattttatt tggggtcatt ccctgatatt acgggcacta
     4621 tttattcaaa actctgacga aaaacaggct gtcgtcagtt ttgacgtgac gaaacgaaat
     4681 accgcgtgac agccatcacg cggcagacat tttatttttt ctcgacgacg ggagccgcct
     4741 gatacccgcc gcccagcgat ttcatcaact gaatgctttg gatcacccgg cggctgtcca
     4801 gcatcagtaa tgacatctct tcggcaagta ctggcaaccg ggcttcagtg gcctgtaagc
     4861 ggctggttaa gccgcgctga taggcggcct cggcagcgcg ctgggtgaag cgcgttgctt
     4921 ccacgtgttc agcctgcatt tctcgctcgt cgttgagcgt ttgcagacgc gtgccgttga
     4981 cagcaacgtc acgcaccgcg ttcagtactg actggttgta acgttcaatc atcatgttgc
     5041 tggcggcgcg cgtgccttcg agattggcat tcaaccgtcc accgtcaaac agcggcaatt
     5101 tcagacccgg gatgaagttg aactggcgac tggttttttt gaataaggta tccaggtgga
     5161 tggcatcaag gccgaaaaac gctttgatat caaagctcgg atagaacaac gcccgcgcgg
     5221 aatccacctg atctaatgac gcctgaacat accagcgcat ggcttgcaga tccgggcgtc
     5281 tggcgagcaa ctcataagag agtgtcgccg gaatgccggt ctggactcgt ggtaatgcca
     5341 caggtttgat ctccggcata tcgctggctc ccgcgccaat caatgcgcgc agagattctc
     5401 tcgtttcggt gatttgccct ttgacggcag caatttgttt atcgaccgcc agaatctgtg
     5461 cccgcgcgcc gtggaaaggc acttgtgctt ccagaccgtg cgccacttta ctttggtgcg
     5521 ctttcaccgc gtaatcaatc acatcgcgag tttgttctaa cagatcgagc atctgatagc
     5581 tggcctgcat actgtaataa agctgcgcta cgcccgtggt cagcgatagc tctactgctg
     5641 cggtttctgc cagcgcggca ttatgcgcgc caatggcggc ggcaaccgct gagcgatgca
     5701 caccccacaa atcaagatcc agtccggcaa acaaacctac tgtggcttcc gtatagtacg
     5761 gcccgtccat ccccagtgcg ggcgcatcca tcgcataagg gcttaaaaag ccgttcgccg
     5821 agacacgttg gcggttaagc atccctaacg ctgccacctg caactgtgaa ccggcatcta
     5881 acaaatcggc ctgcgattgc gctttttctt cccgcagttt cgcttcggcg agggtgtgtg
     5941 aaccacttag cgtccgttgg atcagggcat ccaactgcgg gtcattgagt tgtttccacc
     6001 actgcgcctg cggccagccg gagctggcaa gatgaatatc atcggccagt ttgatttgtt
     6061 ccggtttgag ctgttgatga ggcgcagaat ccttacgtac cagggcacag ccggaaatca
     6121 gcgtcgtgct gccgagaatg ctgcacaaca gcagacgtga aagttgacga ttgatcattg
     6181 cgtggctcct tgtgcctgtt ccgtggcggg cgttaatgcc ggggctgtcc agtctggcaa
     6241 gcgggcgaaa agctggcaga cgtgttgctc ctgttttaat aaggtgggca ggatggcctg
     6301 cgaggtgggt gtctcttcaa gcgttatttg aggagaacgg ctgagtgcgg ttgccagacc
     6361 ggcagcgtat ttctccaggg cgtcggcaaa ctgacctgct ctgtccggct gtagtgcgtt
     6421 atccagcgcc tgcgccgaat tccaggtggc atcccaggcg tgaaggatat cgcggccctg
     6481 acgaataacc gtttgcgaac gttcaatcag taaggcgcgt tcttcgctgt ccagttgacg
     6541 ctccagcgcc acgcgttggc acatctcttc acaggcatta aacgccgcat gcagaccgat
     6601 acgaatttgc agataagtgc gcagagccgt gacttcctgc tggcgtggga tccgcattac
     6661 tttacttaac atacccagcg cgccagccag tttttgcggc agcgtgcgcg cttcactttc
     6721 aggccagaca aaggtgtaaa tcatcgcgga caccacggta ccaatgatga tacccagggc
     6781 gcgatcgcga atttccacca ggtcgtacac tgggccaaaa acgttttcga gcgtggcgag
     6841 cgcgaaggtg accaccatct gtgtgccgat ataagaagag cgttcagagc tggtggcaat
     6901 ccatgcgccc aacaggaaaa tcggtgccag cacaaacagc aattcgacaa tattgtccag
     6961 ccagggcatg accagtagcg tgaataacag cgccagaatc gcgccgcaaa aggccccgcc
     7021 aaaacgcagc accatcttct ggtacgacga accgacattt ggattagcga cgatcacgca
     7081 ggtcagcata caggtgtgaa tgccttccca gtccacgccg ctgtaaaagg tgtaacagat
     7141 caaacaggcg agcaatgttt ttaccgcgta gcgtatatag tctggattgg taaaagcatc
     7201 ggcgaccatt gatggcggtt tggcggcggg cgttggcggc gtattcgggt tcatctggcc
     7261 cagttgtaac aacgtctggc agatattctc caggttacat tcccgtgccg ccaccgcttc
     7321 actttcactg agccgccagt cgctttgcca gcactgacct tcagcaacgg catgctgcag
     7381 cttgttgatt tctgaggcta atttttgccg aaattcaata attgcctgag aatcagcaaa
     7441 agaggtggga tcgtagcgat tcagcgtcga gtaaatgtag gttaccgttg ccacgcagct
     7501 ttgccaccat gcgctttgag ttcgccagtt ggcatcgtcc gcgagacaaa agacattcag
     7561 tttttgcagc gccagcgcct ctctttcaat ccgcgtttcg ggtagcggtg cgaggctgtc
     7621 cgtcaggtgg ctaatggcat catcaagccg atcattaagc gcttgatgca tttgcgtaat
     7681 ggcacgattg ggaaaccaca gcacgccgat taacgtcatc agcaaggttg gatagaggcc
     7741 aacaacgata caccacagcg ttaagcgcac gactacttcc ggatagtcga gcatggcggg
     7801 gaaggtttgc ccgtaaatgg cgacagcgaa aaagaccagt cccagccgat gggtgcgcat
     7861 caaaaacatg cagcccatca ggatcggccc ggcgatgatc aatcggatca acggttcgcc
     7921 gtatgaccat ttgtagatca aaaacaggct gccgatctcc agcaccgtgg caaccacaaa
     7981 caagatcgcc acaaatttgg tgtaaaacgc gttcgactga ataccgtaaa acagcactgc
     8041 cagcgataac gccacaaaag ggatctcaaa agtcatcgag atcagaatca ccagcaggca
     8101 gcctacccag agttgcacga tctgcggcac gcggccgggt cgccgctcgc ttaactcttc
     8161 atgaaagaac gccagcagcc tgaccaccgg taatggcagg gagttgagcg cgctcattat
     8221 tgcggctcaa gattagcgac tgccgaagcg ccgatgcgga acatttccgg gtcaggttta
     8281 tcgaccatga ttttgaccgg aaaacgctgg gcaacgcgga cccagttaat agaacgagac
     8341 acttttggca ggccgcccag caccaggccg ccgtcatccg gtagcacgcc gtagccaatc
     8401 gaatccactt taccctcgaa ggttttgccg ctgtcactca tcaggcgaat cgttgcgggt
     8461 gtacctgagc gaatattttt cagatcggtt tcgcggaagt tggcgatcac gtaccagtga
     8521 cgagtgtcga tgagggtaaa aatagggcgc atggcagaag caaattgccc gacggaggtt
     8581 ttgagggaaa tgatccggcc atcaaatggc gcgcgaacgg tcgtcatttc cagatgtagt
     8641 ttggtcaggg caatatccgc ttcgaccgcc gcacgttgcg ccaccagcgc atcgacgccg
     8701 ctgacggcgc tggcggctga ctgcgcctgt aacaatacgg cattaagatc cgcttctgcg
     8761 gcgcgctgcg ccgttcttgc acggtcaacg tcttctgctg agacaaaacc ctctttcagt
     8821 aatggctcgg tgcggcgtaa tgtatctgtg gcctgtttcg cggcggcacg ggctttttct
     8881 accgtggcat taaccgagtc ggcaccaaac tgttgggcgt caacgctacg ctgggtgagc
     8941 ataatttgct tatccagcgc cgcgagggag gcttcggctt tcgccaggtt ggcttcgtac
     9001 gggcgcgggt cgatgcggaa cagcaaatcg ccctgtttca ccgcctggtt gtcggtgacc
     9061 gccagttcta caatgcgacc gctgacttcc ggcaccacat caatggtatc tgctgacgcg
     9121 taagcgtcat tagttgatgg cgcactgtct acgcgccaga taacgaaaac aagggcaacc
     9181 agcgccaacg caaccactaa cagagcaggg aatttactgc gaggtgcttt tttcggcgta
     9241 ctttccatga gcgcgtctca aatagattag aaaaatgcca gccagaacag catggcgtag
     9301 agagcaaaaa gggcggtata aataattccg acaaatgcca gattgatatt tgcccg
//
LOCUS       DAZSNO010000152         9291 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZSNO010000152 DAZSNO010000000
VERSION     DAZSNO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.152"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="ACHWN2_003364"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="ACHWN2_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR8932772.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="ACHWN2_003365"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="ACHWN2_003365"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR8932773.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHWN2_003366"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHWN2_003366"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR8932774.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHWN2_003367"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHWN2_003367"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR8932775.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHWN2_003368"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHWN2_003368"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR8932776.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHWN2_003369"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHWN2_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR8932777.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="ACHWN2_003370"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="ACHWN2_003370"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR8932778.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="ACHWN2_003371"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="ACHWN2_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR8932779.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="ACHWN2_003372"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="ACHWN2_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR8932780.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="ACHWN2_003373"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="ACHWN2_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR8932781.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2384 a         2327 c         2357 g         2223 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgccatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat t
//
LOCUS       DAZSNO010000153         9237 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZSNO010000153 DAZSNO010000000
VERSION     DAZSNO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.153"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="ACHWN2_003374"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="ACHWN2_003374"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR8932782.1"
                     /translation="MKALSKLKAEEGIWMTDVPVSELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="ACHWN2_003375"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="ACHWN2_003375"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR8932783.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="ACHWN2_003376"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="ACHWN2_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFR8932784.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="ACHWN2_003377"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="ACHWN2_003377"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR8932785.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="ACHWN2_003378"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="ACHWN2_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR8932786.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="ACHWN2_003379"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="ACHWN2_003379"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR8932787.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="ACHWN2_003380"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="ACHWN2_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFR8932788.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="ACHWN2_003381"
     CDS             complement(8064..9206)
                     /locus_tag="ACHWN2_003381"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFR8932789.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         2556 a         2153 c         2034 g         2494 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccgatac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatt
//
LOCUS       DAZSNO010000154         9186 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZSNO010000154 DAZSNO010000000
VERSION     DAZSNO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.154"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..923
                     /locus_tag="ACHWN2_003382"
     CDS             <1..923
                     /locus_tag="ACHWN2_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8932790.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHWN2_003383"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHWN2_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR8932791.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHWN2_003384"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHWN2_003384"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR8932792.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="ACHWN2_003385"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="ACHWN2_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR8932793.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="ACHWN2_003386"
     CDS             4446..4805
                     /locus_tag="ACHWN2_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR8932794.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHWN2_003387"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHWN2_003387"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR8932795.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="ACHWN2_003388"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="ACHWN2_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR8932796.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="ACHWN2_003389"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="ACHWN2_003389"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR8932797.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2102 a         2222 c         2483 g         2379 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaagcgtatt atgcacaccc cgcgcagctg agaaaaagcg
     9181 aagcgg
//
LOCUS       DAZSNO010000155         9102 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZSNO010000155 DAZSNO010000000
VERSION     DAZSNO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9102
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.156"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHWN2_003390"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHWN2_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR8932798.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHWN2_003391"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHWN2_003391"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR8932799.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHWN2_003392"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHWN2_003392"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR8932800.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHWN2_003393"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHWN2_003393"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR8932801.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="ACHWN2_003394"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="ACHWN2_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR8932802.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /gene="smg"
                     /locus_tag="ACHWN2_003395"
     CDS             5562..6035
                     /gene="smg"
                     /locus_tag="ACHWN2_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR8932803.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="ACHWN2_003396"
     CDS             6064..6606
                     /locus_tag="ACHWN2_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR8932804.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHWN2_003397"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHWN2_003397"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR8932805.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="ACHWN2_003398"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="ACHWN2_003398"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR8932806.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="ACHWN2_003399"
     CDS             8003..8260
                     /locus_tag="ACHWN2_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR8932807.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="ACHWN2_003400"
     CDS             complement(8236..8790)
                     /locus_tag="ACHWN2_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR8932808.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2284 a         2208 c         2270 g         2340 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagaga aaaaatcctg aaattcaggg ttgactctga
     9061 aagaggaaag cgtaatatac gccacctcgc gacagtgcgc ta
//
LOCUS       DAZSNO010000156         9079 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZSNO010000156 DAZSNO010000000
VERSION     DAZSNO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9079
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.157"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(227..2770)
                     /gene="mdoH"
                     /locus_tag="ACHWN2_003401"
     CDS             complement(227..2770)
                     /gene="mdoH"
                     /locus_tag="ACHWN2_003401"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR8932809.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2763..4298)
                     /gene="mdoG"
                     /locus_tag="ACHWN2_003402"
     CDS             complement(2763..4298)
                     /gene="mdoG"
                     /locus_tag="ACHWN2_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR8932810.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4692..5849
                     /gene="mdoC"
                     /locus_tag="ACHWN2_003403"
     CDS             4692..5849
                     /gene="mdoC"
                     /locus_tag="ACHWN2_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR8932811.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5857..7338)
                     /gene="clsC"
                     /locus_tag="ACHWN2_003404"
     CDS             complement(5857..7338)
                     /gene="clsC"
                     /locus_tag="ACHWN2_003404"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR8932812.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7280..7813)
                     /gene="ymdB"
                     /locus_tag="ACHWN2_003405"
     CDS             complement(7280..7813)
                     /gene="ymdB"
                     /locus_tag="ACHWN2_003405"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR8932813.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7908..8219)
                     /gene="ymdA"
                     /locus_tag="ACHWN2_003406"
     CDS             complement(7908..8219)
                     /gene="ymdA"
                     /locus_tag="ACHWN2_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR8932814.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8340..8672)
                     /gene="csgC"
                     /locus_tag="ACHWN2_003407"
     CDS             complement(8340..8672)
                     /gene="csgC"
                     /locus_tag="ACHWN2_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR8932815.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8731..>9079)
                     /gene="csgA"
                     /locus_tag="ACHWN2_003408"
     CDS             complement(8731..>9079)
                     /gene="csgA"
                     /locus_tag="ACHWN2_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR8932816.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT         2277 a         2349 c         2157 g         2296 t
ORIGIN      
        1 gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga gccagtcagt
       61 tgcttaactg actggcatta cggcataacg ccggtatttt tattggctcg ttgtttttgc
      121 cggatgtggc gtgaacgcct tatccgacct acaaaatctt gcaaaatcaa taaattgcag
      181 gaacgatgta ggcctgataa gcgtagcgca tcaggcaact acgtttttat tgcgaagccg
      241 catccggttt acgcaatgcc agtggattga gctttatccc ttcgtaataa ctcacccatg
      301 aagaatatct ttcaggggaa ttccagacgc ggaaatgcag acgggccatc gtcaccggat
      361 cgcttagcag caccaggcga cgatcgcgat tcagcttctc tggcgtctcg ttaagcgcct
      421 gttcaacgtg gcggtcacgg gcgatttcca gcaccttact ggcgcggtga cgcgctgtcg
      481 ccattgcggt tgccagagcg ttaaatgacg ggttaaacac tgcgtgcata aagccatcgt
      541 caagggagcg ttggcgattc atctcaagga accgatcggt atcaaccagc acctgcggcg
      601 gtgaatactc ttccgggatc aggaacagtt tccagcgttt ggtgcgcaga ccaacggtgg
      661 cacggctgga aatcaccgaa acaaacggtg acaggatcaa cgagaagaca atcggtgcca
      721 gccagaacag gaaacgcaga tccagccacg ccatcccaac agcccacact aaccccaaca
      781 gcagctgtga gccgtggcgt ttgaacgctt cgccccatga agtggagtca tcatcacgct
      841 gcggtgaatt ccacaccact tcccagccaa ggaacgcgct gacaacgaag accgtatgga
      901 acagcatgcg taccggagcc agcagcacgg aaaacagcac ttccagcagc aacgataatg
      961 taacgcgcca gaagccgccg tattctttcg ttcctttgca ccagataagc aaaatgctca
     1021 ataacttcgg caggaacaac agcaccatgg tcgaagcaaa aagtgcaatc gccagctcag
     1081 gacgccactg cggccacacc gggaacaact gccgtggttg caggaagtat tgcggttcgg
     1141 tcaacgcatg cactacctgc aatgcagtag agagcgcgag gaacataaac cacagcggag
     1201 cggagagata agacatcacg cccgtcagga acaccgcacg gtgaaccggg tgcataccct
     1261 taaccaggaa cagacggaag ttcatcaggt taccgtggca ccagcggcgg tcacgtttta
     1321 gctcatcaag caagttaggc ggcaattctt cataagaacc cgggagatcg taagcaatcc
     1381 agacccccca acctgcacgg cgcatcaacg ccgcttctac gaagtcatgt gacaggattg
     1441 aaccggcaaa ggaaccttcg cccggcagcg gagccagtgc gcagtgctcg ataaatggtt
     1501 tcacgcggat aatcgcgtta tgtccccagt agtgcgactc gccaagttgc cagaagtgca
     1561 aaccggctgt aaacagtggc ccatacacgc gggtcgcgaa ctgctgacag cgcgcataca
     1621 gcgtatccat accggacgct ttcggcgacg actgaatgat cccggcgttc gggttggctt
     1681 ccatcaagcg caccaggccg cataaacaat caccggtcat taccgagtca gcatccagca
     1741 ccaccatgta gctgtactgg ctgccccagc gacggcagaa gtcatcgata ttaccgcttt
     1801 tacgcttcac gcggcgacgg cggcggcgat agaaaatctg accttcgcca ccgacttcag
     1861 cgataagctc catccaggct ttttgctctg cgacgcagat atccgggtta taactgtcac
     1921 tcagaatgta gacatcaaag tgtttggcat tcccggtggc ttttactgat tcccacgttg
     1981 cacgcaagcc agcaaaaaca cggttcacgt cttcgttaca gataggcatg atcaacgccg
     2041 tgcgatgctc cgggtttaat ggttcatcgc caactgttga cgcagatata ctgtatttat
     2101 cgcgaccaat aagcagttgc aggaagccca tcaacgccgt ccagaatccg gcggacaccc
     2161 aacagaacag taccgcaaaa aggatcagga taccggtttg cagcatataa ggcagaagct
     2221 gcataaagga aacccacaaa tcctgaccaa ccatatccat aggattaatc agcgcccaac
     2281 cctgataagg aagaatggtc ttcatatacc aggtcgcgac aacagtttgc gcgagcgtca
     2341 ggatcaacag aatgtaacgg cggatggtac caacggtacg ccacttttgc tcgctctcct
     2401 gctcttcttt ggtcaaacga gccagatagc gcggcgtgac atcgcgtcca cgcaggcgat
     2461 cccagaaacg gcctaccggg ttggtacgcc acgggtcggg aaacatcgag gagcgttttg
     2521 cttctggcat cgccttaagc tgatcgcgcc cttcgtcgtc tttaattaac tgtccatcag
     2581 caagtgaatc tggccaggct tgttccagac gcgcctttac cgagccttgc ggggaatcat
     2641 cctcccgcgc ccaggtgcgg tgttcggcat ccagcgcctg atgaacggcg cggatatcag
     2701 tcttcggcaa tgctgctttc tcgcttgcgg cgatgggcat tgcgtcaatg tactcagttg
     2761 tcttattcat tggcaggtaa ctggtagctc caggtttcac tcaacgtctg atcggcattc
     2821 accagcgcag cacgcatttc agtgattttc ttggcatctt tcactttcac acgcatcacc
     2881 agacgccagc ctttggtaac cgggttatag cgcaccgtgc tttcaactat ctcaccatta
     2941 tcaccaatgc tggtttgcgc tgtgaccggg gtatcctctg gcagtttttt catctctgcg
     3001 ccggtaaaat cgaccacaaa ggcgatagta ccgtcaggct ggcgaatcag gttcgactgc
     3061 ttcacatccc ccgttgaacg acgcgtttgt tgcacccatg cgttatctgg cgcatgcagt
     3121 ttgtcttcat cacggctgaa ggtgatggtg tatttaaagt tcatctcttt acccggctcc
     3181 ggcagctgat ccggcgtcca gtaagcgacg atgttatcgt tggtttcatc gttggttgga
     3241 atttccacca gctcaacgct gcctttgccc cactcccctt tcggagtcac ccatgcgctt
     3301 ggacggagat cgtaacgatc atcgagatct tcaaagcggg agaaatcacg acctcgttgc
     3361 aacagaccaa agcctcgcgg gttttccatg gagaagctgc tgaccgctaa atgtttcggg
     3421 ttattcaacg gacgccagat ccattcgcca ttaccagcat ggatagacag accgttagag
     3481 tcgtgcaact ccggacgata gttatttgca ggcgacggtt ggttcggccc aaacaggaac
     3541 atactggtta acggtgcaac ccccagtttg ccgactttat cgcgcagata gattttcgac
     3601 tgcacatcca caaccgtgtc acgccctggc ataaccacga atttgtaagc acctgtcgca
     3661 cgcggcgagt caagcaatgc ataaatggtt aaacgtttat cagtcggttt tggacgctcg
     3721 atccagaact ctttgaagcg tggaaattct tcacccgatg gcaaagcggt atcaattgct
     3781 aggccgcggg cagaaaggcc ataaacctga cctgcaccaa tcacgcggaa atagctggcc
     3841 ccgagcatgc tgacgatttc atcgttttta tctttgctgt tgatcgggta aagcacttta
     3901 aaaccggcaa aaccaaggtc ttttaccgtg tctttgtcat gctgaacatc gccgaaagtg
     3961 aaataatccg ggctgtattt gattcgtttg actgcggtgg cagtcacttc atttattttg
     4021 accggggtat cgaagtacat accctgatgg tagaactcga gtttgaatgg ggtcttcaga
     4081 ttgttccagt acgctttgtc atgattaaac tggatctgct gatagtccgc gtatttcatg
     4141 tcgcggaaaa cggagggcaa gttgctttta ggcgcctcat agcctttccc ggctaaggat
     4201 tgagcttgct ttgcgacatc atcaatactg aaagcccagc ttgaagatgt atacagggtt
     4261 aacattactg cagcactcaa ccaacgcatt ttcatcattt gtagtttatg tttcataatt
     4321 agtaagcact tccccctttg tgtgcttata tcgatccgat ctattttaat ggataaccag
     4381 ccattccgac aactgaatcc cggtatcgtt cagcacgctg gctcatggat taagcacctg
     4441 attgcaaccg ttttcacttt ccgtgcttct cctaaagaca gaagcatcga cttcgtgtag
     4501 ggtttgccag caaatttatt tatcaaccgt cttacagacg cttccattct ggttagtggt
     4561 agcgatgatg ctatgcataa aaaccttgaa gcctgcgcct ggcgcggtca ggtcggtgtc
     4621 gctatggcaa caagcctaaa ggcataaccc gacagggata agacgaaaaa tccgagatta
     4681 gttaaccata tatgaatcca gtacccgcgc aacgtgaata tttcctcgac tccatccgcg
     4741 cctggctgat gttgttaggg atcccttttc atatttcttt aatctattcg agccatacat
     4801 ggcatgtgaa tagcgccgaa ccgtcattgt ggctaaccct ttttaatgac ttcatccact
     4861 cgttccgcat gcaggtattt ttcgttatat ccggatactt ttcctacatg ctttttttac
     4921 gctatccctt gaaaaaatgg tggaaagtac gtgtcgaacg tgtaggtatc ccgatgttaa
     4981 cagccatccc cctactgaca ttaccgcaat ttattatgct gcaatatgtc aaagggaaag
     5041 cggaaagttg gcctgggcta tcattgtatg acaaatataa tacgttggcc tgggaattaa
     5101 tatcacacct gtggttttta ctggtgttag tagtcatgac gacgctgtgt gtatggatat
     5161 ttaagcgcat cagaaataat ttagaaaatt ctgataaaac gaataaaaaa ttctcgatgg
     5221 taaaactatc ggtgattttt ttatgcctcg gcatcggcta tgcggtaata agaagaacga
     5281 tttttattgt gtatccaccc attctgagta atggcatgtt caattttatt gtcatgcaaa
     5341 cgctatttta tttaccgttc tttatcctcg gcgcactggc tttcattttc cctcatctta
     5401 aagccttgtt taccacgccg tctcgtggct gtaccctcgc tgcagcattg gcgtttgtcg
     5461 cttatttact caaccagcgc tatggcagtg gcgatgcctg gatgtacgaa accgagtcgg
     5521 tgatcaccat ggtcctcggc ctgtggatgg tgaatgtggt cttctccttt ggccaccgtt
     5581 tgcttaactt ccagtcagcg cgggtgactt attttgttaa cgcatcgctg tttatctatc
     5641 tggttcacca cccgttaacg ctgtttttcg gcgcatacat tacaccgcac atcacctcca
     5701 actggcttgg ttttctctgt ggcctgatat tcgtagtagg gattgcgata attctgtatg
     5761 aaattcattt acgcatcccg ttactgaagt ttttgttctc tggtaaaccg gttgttaagc
     5821 gtgagaacga taaagcacca gcccgttaag ccacatttac aataaccatt ccacgggcaa
     5881 tatcgacgcc agtctgacca taacccgctt ccagaaactg gtggcgggtt cttttttgag
     5941 aataacctct ttctttgcat gacgatcaac ccagttgatc cgtccccacc tgtccagacg
     6001 gagctgccag gccgcatcgt actggctctg aataaagcgt ttatcaatta actgtgccag
     6061 cgtttcgctc tctatcacga agcccatttc agtattgagc aatgttgaac gcggatcgaa
     6121 attgaaagag ccgataaaca ccgttttacc atcgatgcta aaagttttag catgcaggct
     6181 ggcaccggaa ttaccggtta tgccgcgatc gtgtaacgta ctgctttgtt cacgcgtcgg
     6241 cttgagttca tataattcca cgccatagcg gagcaatttt ttgcgccagc gcgcgtatcc
     6301 ggcatggacg acagcaacat cgttagcggc aagagaattg gttaggatcg caatctttac
     6361 cccttttctc accatccgta agagttgcgc cacacctgcg cgtgtcggta caaaatagga
     6421 agagataata tcgatgcgtt cactgggtga gcccatgata tcgaacaggc gctgcggtaa
     6481 cagtgaatgc cgttttgcct tgccctcccc tttcgccgga tcatcactta ataaacgtgt
     6541 cttcgcccag ataagcggca acgttccatc aaccagatga tttataaatg gactggattc
     6601 cattttgcgt aaataacgat gcgtcatggc atcgttatgc caggaggcgg gtaactcgat
     6661 gcgatccgcc atttcacctt ccgggacatc cagcacctgc tgtaagggtg aaaccgattt
     6721 gcaataccag tagcgggcga aatcatcggc aacatcctct accacggggc ctattgccat
     6781 gacatctaaa tccgaaaaaa gtggctcttc ccctgcacca aaataggcat caccaatatt
     6841 tcgtcctccc actagggtaa ccacgccatc gacagtgaaa cttttattgt gcatccggcg
     6901 attgagacgg gaaaagtcgg tgatatatcc aagcggacgt agcaagcgaa acgagaaagg
     6961 attaaaaagc cggacttcaa tgcgtggatg actgtcaagc aagcgcaaaa tgtcgtcaag
     7021 tccgggcgta ttgttgtcgt ccagcaacaa acggacgcga acgccacgct ttgctgcggc
     7081 taacagggcg gaaaacagta accgccccga catatcgtcc tgccagatgt aatactgaac
     7141 atccagcgta tgctctgcca tttcggccag acgataccgg gcggcaaacg catccagact
     7201 tttctccaga gggaaaagac cacactgacc gggatgttgc gaacacagtg gcagcaccgc
     7261 gctcgccagc cggggcaaat cattcatctc cttgttgggt aaggagtctt tcgtagaggt
     7321 gggcgttttc ttcatcataa cagacaaagt atacctgttc gggtaaagcg tgacgggtaa
     7381 taaattctga aacggtttta actgcgattt cagccgctgc cgcacgaggg taactataaa
     7441 ccccagtact gattgcagga aaagccactg acgtatagct gtttgccgcc accagtcgta
     7501 ggctattcag ataggcatcc tgcaaaagct ggtcttcgtt ttgttcccca ccacgccaga
     7561 ctggcccgac ggtgtgtact acggctttag cgggaagatc gcctgcaagc gtaataacgg
     7621 catgccccgt agggcaatcg ccctgctgtt gcctgacttt taaacaggca tccagcaggg
     7681 ccggacccgc tgcgcgatga atggccccat cgacgccgcc gcctcccatt aatgacggat
     7741 tagccgcatt cacaatcaca tcaacggcca gtttggtaat atcaccctgc acaacatgaa
     7801 tacgcgtttt cataacgcct ccttacacct tcttgtttct taagtgtaca gcaggctttt
     7861 tggttaaatc gcatttttct gctcagcaac gtgagttgtt cagaaactta gttatattcg
     7921 atgttcatta cagccagctt tttctgttta tcaagatact gcacttttac tgaggctatt
     7981 tgcgcgtttt tgacattgcc gctcattaac gctttgctgg agtaggtttt gcccggaata
     8041 gaaccattct gtgggcagtt catttctacg ggtgatgact gggtgctgac gtcacatgcc
     8101 ggttccacaa tttggccata aaaatggatc acgccccctt gcgcggtgct tcctgcaatg
     8161 gcaataaaag gaaaaaacaa actgccgagc ataagagagt ctaaaaaagg tctgaacatt
     8221 tattctattt cctcaatgat tagtcatcct tgaaggttgt gttatccata ctttcaggtt
     8281 ggctgattcg cagcagacca ttctctccag attcatctta tgctcgatat ttcaacaaat
     8341 taagactttt ctgaagaggg cgtccattgt tgtgataaat gaagtgcctg tccatcagaa
     8401 acagtaacaa ctattttcac ccgatcttcc ggggaaatat ttaaactcaa cttcgtcaaa
     8461 gcaatgggtt gattagcagg caatgagagg gtcttttctt gcttcgtctg actttgccct
     8521 gaactgcctt cgcgcaggga caatatttgt actctgcaca gacaagattg agtaagagta
     8581 acttcaggaa taatggtgta catatcccct tgctgggtcg tattaaaggt tatctggctg
     8641 gaaagtgccg caaggagtaa taacgcattc atattcttct cccgaaaaaa aacagggctt
     8701 gcgccctgtt tctgtaatac aaatgatgta ttaatactga tgagcggtcg cgttgttacc
     8761 aaagccaacc tgagtcacgt tgacggagga gttcgatgca gtctggtcaa ctgcagcacc
     8821 gttgccacca ccgaactgtt taacagtcat ttcagaattt ttgccgttcc actgatcaag
     8881 agtggcgctg ttaccgaagc cacgttgggt cagatcgatt gagctgtcat ctgagccctg
     8941 accaacatct gcaccattac caccgccatg ctgggtaata gtcaagtcag agttacgggc
     9001 atcagtttgc agagcaagtg cagagttacc gccaccgtac tggtaaatgt tcagctctga
     9061 atttgggccg ctattatta
//
LOCUS       DAZSNO010000157         8870 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZSNO010000157 DAZSNO010000000
VERSION     DAZSNO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.159"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            5..2177
                     /gene="espL4"
                     /locus_tag="ACHWN2_003409"
                     /pseudo
     CDS             5..2177
                     /gene="espL4"
                     /locus_tag="ACHWN2_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2146..3883)
                     /gene="aceK"
                     /locus_tag="ACHWN2_003410"
                     /pseudo
     CDS             complement(2146..3883)
                     /gene="aceK"
                     /locus_tag="ACHWN2_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4067..5371)
                     /gene="aceA"
                     /locus_tag="ACHWN2_003411"
     CDS             complement(4067..5371)
                     /gene="aceA"
                     /locus_tag="ACHWN2_003411"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFR8932817.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5401..7002)
                     /gene="aceB"
                     /locus_tag="ACHWN2_003412"
                     /pseudo
     CDS             complement(5401..7002)
                     /gene="aceB"
                     /locus_tag="ACHWN2_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7271..8200)
                     /gene="metA"
                     /locus_tag="ACHWN2_003413"
     CDS             complement(7271..8200)
                     /gene="metA"
                     /locus_tag="ACHWN2_003413"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR8932818.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8357..8800
                     /locus_tag="ACHWN2_003414"
     CDS             8357..8800
                     /locus_tag="ACHWN2_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR8932819.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2309 a         2156 c         2154 g         2251 t
ORIGIN      
        1 tcgtattcct cgtagttctt tctctgcaaa tattaataat acagcccaga caaatgaaca
       61 ccaaaccctg agtgaattgt tttataaaga actcgaggat aaattttctg gcaaggagct
      121 ggcgacgcct ctattaaaaa gcttctcaga gaactgtaga caaaatgttc gtcatatctt
      181 tagcaacaag gattttgtca ttaaattttc cacgtccgtc ttacaagctg ataagaaaga
      241 aattacgatg attaataaaa acgaaaacac gacactcact caaaccattg ctacaatatt
      301 tgaagaatac ctaatggaaa ttttacctca acgctcagac actcttgata aacaagaatt
      361 aaaccaaaaa tcagatagaa aagaaaaaga attcccaaga attaaactta atggtcaatg
      421 ttatttcccg gggcgcccca aaaccgtata gtataccgac acattgctgc acaatatatt
      481 aatgatattt atcagaatgt tgattacaaa ccccatcaag atgattactc ttcagctgaa
      541 aaatttctca ctcacttcaa caaaaaatgc aaaaaccaga ctttggcgtt gatttccagc
      601 cgtcctgagg ggcgttgcgt tgctgcctgc ggtgatttcg ggctagttat gaaagcatat
      661 tttgacaaga tggaatcaaa tggcatcagt gttatggcag ccatattact ggtggataac
      721 catgctttga cggtccggct aagaataaag aacacaactg aaggatgtac ccattacgtg
      781 gtttcggttt atgatcctaa tgtaactaac gataaaataa gaattatgag cgaaagcaaa
      841 gaggatatta aacactattc tctgatggat tttatgaatg tagattatag cctcctgaaa
      901 tggtcaaatg atcatgttat taaccaatct gttgcaataa ttccagcact tccgaaagaa
      961 cagctattga tgttaaaagg aactgtggat gaaataaccc ctccattatc accagcaacg
     1021 atgaatttgc taatggcaat tggtcagaat caccaactta cgcaactgat gattcagctc
     1081 cagaaaatgc cagaactaca tagaacagaa atgttgactg cctataatag tattaacctc
     1141 cccggtttat atttggctat aaattatggc aatgcggata tcgttgagac tattttcaat
     1201 tcattgtcag aaacaggata tgaagggtta ctctcgaaaa aaaatctcat gcatattctg
     1261 gaggcaaaag ataaaaatgg tttttctgga ttatttttag cgatatcacg taaggataaa
     1321 aatgttgtaa cctcgattct gaacgtctta cctaaactgg ccgcaacaca tcatttagat
     1381 aatgaacagg tgtataaatt cctgagtgcc aaaaatagaa cgtccagcca tgttttatac
     1441 catgttatgg cgaatggtga tgccgacatg ctgaaaattt ttttggtcgc gttacctttg
     1501 ttaattcgca catgtcattt gactaaagaa caggtactcg atctcctgaa ggcaaaggat
     1561 ttttatggtt gcccaagact atacctggcg atgcaaaatg gacatagcga tatcgtgaaa
     1621 gttattctcg aagcattgcc ctgtctggcc caggaaatta acatttcagc ttccgatatt
     1681 gtagatcttc tgaccgctaa aagtcttgcg cgcgacacgg gtttgtttat ggccatgcag
     1741 cgcggacata tgaacgttat taatactatt tttaacgcat tacccactct ttttaatacg
     1801 tttaaattcg ataaaaaaat atgaagcccc tcctcctggc aaataattct aatgaatatc
     1861 ccggtttgtt ttcagcgata cagcataaac aacaaaacgt tgtagagacg gtttatcttg
     1921 ctttatctga ccatgcacgc ctgtttggat ttaccgctga agatattatg gatttttggc
     1981 aacacaaagc gccacaaaaa tactctgcct ttgagttggc ttttgaattt ggtcaccggg
     2041 ttattgctga attaatcctt aatacattaa ataagatggc tgaaagcttt ggctttacgg
     2101 ataaccctcg atacattgcg gagaaaaatt atatggaagc tttactcaaa aaagcatctc
     2161 cccataccgt acgctaaatc tttgcctgcg ccgatacgca taaacatctt ccacatgccc
     2221 ttcacgtatg cggttttgta gtgcgcgcca gtaatcagcg cggaacaggt cggcgtgcat
     2281 ctcttcaaac agcggaccaa tacgcgggtc ggcgcatagc cagtggcgaa actcttccgg
     2341 gaaaacatcg cccggcgaga cgctgtacca cggttcgctg gcaagttcgt cttccggata
     2401 gcgcggcggc gggatgtcgc ggaaattcac ttccgtcatg tagcaaattt catcgtaatc
     2461 ataaaaaacc acacgcccgt gacgggtgac accgaagttt ttaaacagca tgtcgccagg
     2521 gaaaatgtta gcagcggcaa gctggcgaat agcgttaccg tattcttcaa tggcgtcgcg
     2581 caactgctga ccttccactt gttccagcca gatattgagc ggcaccatcc gccgctcgat
     2641 tatagagatg gcgaattaca atctgctcac caagatcggt gattttttcc gctgcttcct
     2701 ggagcagtaa ttccattaat accggggaaa tatgccgctt ctccagcaca aagttttcaa
     2761 actcctgggt gtccgccatt cggcccacac ggtcgtgctc tttcaccagt tgatagcagg
     2821 cacgaacgtg agcggcagac atctctttct gcggcgcgaa cttgtctttg atgactttga
     2881 atacccgatc aaagcccggc agcgtaaaca ccaacatcac cattccacga atacccggcg
     2941 cttcaatgaa ctgctcatta cagccctgta gataaacgag atattcgcgg tagctttcgg
     3001 ttttggcgtg cttctggcag ccgatagcca tatacaattc agcagtggtt ttacctggca
     3061 gaatttcccg tagccactcg accagcgctg cgggcagcgg tgcataaacc ataaaataag
     3121 aacgcgcaaa gccaaaaaca atgctcgctt cggcggtcgt cgtcaggcag gtatcaataa
     3181 ataactcgcc gtcgtccgtc tggtggatcg gcagtaagaa gggcaacgtg ccggaaggcg
     3241 tgatcagttt gcccaccagc caggcggctt tattgcggta aaacagttcg ttcgccacct
     3301 gtaaatgact ttccgcgagg ttgtctgtcc ccagcgtttc cgtcagatgg cgaatgatgt
     3361 aatgaatgtc acggctttta ttctgccagc gcaggcgcag cggcaggtcg ctgataacgc
     3421 gcatcagtag agattcccag ccgtgatcgg ggtgaaagtc tttcgccagc gggcggggaa
     3481 tggtacgaaa gcggcgctct ggctgagagc taaagataaa aagccgctcg ggagtaagcg
     3541 agcggtggtc aaataaccga cagtacacgg agttaaaaaa gctctccgca atctcgaagc
     3601 gcgggtaatc cggcaacagc cgcgtgtaat gctctttaac gcgcagtaaa aatgccgcgt
     3661 ccgtactttg gccgttagta atgcagcgca gttgctccac gaccagacca acgtgatgat
     3721 cgtaaagatg gatacggttt ttcatcgcct gctggacagc atgccagtcg gcctgttcga
     3781 aacgctgctg cgccccggaa gtcacttcga ggaatcgacc atactgagcg tcgaagcctt
     3841 gcaaaatggt ttgagcaatc agtaattcca ggccacgcgg catttagcgc cctcatcagg
     3901 agcagagaat tgccggatgc ggcgtaaacg ccttatccag cctacgttcg gcaacggttg
     3961 taggcatgat aagacgcgca agcgtcgcat caggcatcgt gcaccgattg ccggatgcgg
     4021 cgtaaatgcc ttatccggcc tacagtcagc aacgtttgta ggttgcttag aactgcgatt
     4081 cttcagtgga gccagtcagc gcggtgactg aagacgtgcc gccctgaata atagtcgtca
     4141 ctttatcgaa gtaacctgta cccacttcct gctggtgaga tacgaaggta tagccatctt
     4201 tcgcggcggc aaattccggc tgctgcactt tctcaacata gtgcttcata ccttcgccct
     4261 gcgcataagc gtttgccagg tcgaacatgt taaaccacat gctgtggata cctgctaggg
     4321 tgatgaactg gaacttgtag cccatatccg acagctgctg ctggaagctg gcaatagttt
     4381 tgtcgtcgag gtttttctgc cagttgaacg acggcgagca gttataagcc agcagtttgc
     4441 ccggatattt cgcgtggata gcttgtgcaa agcgacgcgc cagttccaga tccggcgtgg
     4501 aggtttcaca ccagaccagg tcagcatatg gcgcatacgc caggccacgg ctgatcgctt
     4561 gctcaatgcc cgcatgagta cggaagaagc cttcactggt acgctcgccg gtaataaatt
     4621 cgctgtcata cgggtcgcaa tctgaggtga tcagatccgc cgcatcagca tcggtacgtg
     4681 caaccagcag ggttggaacg cccgtcacgt cagctgccag acgcgccgcg accagtttct
     4741 gaatagcttc ctgagttggc actaaaactt tgccgcccat gtgaccgcat ttcttcactg
     4801 acgccagctg atcttcgaag tgaactgccg cagcaccggc ttcaatcatc gctttcatca
     4861 gttcaaaggc attcaggaca ccgccaaaac cggcttccgc atcggcaacg atcggcagga
     4921 agtaatcgac atagcgcgga tcgcccggct caatgcccac ggaccattgg atctgatcgg
     4981 cacgacggaa ggtgttgttg atccgctcca ccacagctgg caccgagttt gccggataga
     5041 gcgactgatc cggatacatg ctggccgcca ggttagcgtc cgccgctacc tgccatcccg
     5101 acagatagac tgcttcaata cccgctttcg cctgttgcag cgcctgaccg ccagtcagtg
     5161 caccgaggtt gttgatgtag ccttttttcg actcaccgtg cagcagacgc cacattttcg
     5221 ctgcgcccag ttgcgccagc gtgcattcag gattgactga accgcgtaat ttcaccacat
     5281 cttccgcact gtatgggcga gtaatgcctt cccaacgcgg ttgagtccac tctttctgta
     5341 attcttcaat ttgttgtgta cgggttttca tgtgcagatg ctccatagtt atgtggtggt
     5401 ttacgctaac aggcggtagc ctggcagggt caggaaatca attaactcat cggaagtggt
     5461 gatctgttcc atcaagcgtg cggcatcgtc aaaacggccc tgggagaaac gttcttcgcc
     5521 cagttcgctg gcaatgactt tcatctcttc gcccagcatc tggcggaaca aggctttggt
     5581 caccggtttg ccattgctca acgttttttg atgatggatc cactgccaga tcgaggtacg
     5641 ggaaatttca gccgtcgccg catcttccat caggccataa atcggcacgc agccgttgcc
     5701 ggagatccac gcttcgatgt actgcacagc cacgcgaatg ttagcgcgca taccttcttc
     5761 ggtgcgttca ccatcacaag gtgccagcag ctgatcggca gtaatcggcg cgtcttgttc
     5821 gcgcaatact tcaagctgat ttttacggga gccgagaatg tcgttgaata ccgccattgc
     5881 cgtgtccgca aggcctgggt gagcgatcca tgtgccatcg tgaccgttat tggcttccag
     5941 cgctttatcc gcttttactt tgttgagcac ctggttatta cgctcttcat ctttgctcgg
     6001 aataaacgcg gccatgccgc ccatcgcaaa agcaccgcgt ttatggcagg ttttaatcag
     6061 caggcgtgag taagcattca ggaatggttt atccatcgtc acggcctgtc tgtctggcag
     6121 gacacgatcg ggatagtttt tcaacgtttt gatatagctg aagatgtaat cccagcgacc
     6181 gcagttcaga ccaacaatat ggtcgcgcag cgcgtgaagg atttcgtcca tctggaacac
     6241 ggcgggcagc gtttcaatca gcaacgtcgc cttgatggtg ccgcgcggca gattaaagcg
     6301 atcttctgca tagctgaaga cttcgctcca ccaggccgct tcctgctagg actgggtttt
     6361 cggcagatag aaatagggac cgctgccctt tgccaacagt gcctgatagt tgtggaagaa
     6421 atagagcgca aaatcaaaca ggctaccggg gattgcctca ccacgccagg tgacatgttt
     6481 ttccggcaag tgcagaccgc gtacccgaca aatcaaaacc gctggattgg gcttgagctg
     6541 ataaattttg cctgcttcat tggtatagct gatggtaccg ttaaccgcat cgcgcaggtt
     6601 aatttgcccg tcgatcactt tgttccagtc tggtgccagt gaatcttcga aatcggccat
     6661 aaagactttc acattggcgt tcagtgcgtt gatcaccatc ttgcgctcta ccgggccagt
     6721 tatctctacg cggcggtctt ctaagtccgc aggaatcccg cgaattttcc aatcagcatc
     6781 gcgaatggaa gccgtttccg aaataaaatc aggcaacgtt ccgttatcaa tatcttgctg
     6841 ctgctgaatg cgcgctgcca gaagtttatt gcgttgtggc gtaaaatgcg tcaccagctc
     6901 agtcagaaat tctaccgctt cggcagtaag aatttgcttc tcctgctcgc catacggcct
     6961 tgtgaaagcc agttcatcgg ttgttgttgc ctgttcagtc atcgtgcagc tcctcgtcat
     7021 ggatccgaaa acttcccctc tgtgaacgaa ggaacgttgt gcgcttttcg ttcagcacaa
     7081 ctaagactac tcatttaaaa tgcaaaatca aaaacaattt ccattttaat taaaaatact
     7141 tgataactta ttgataacaa tatgaataaa atttattcac ttgctgaggt gcgtttcgga
     7201 aataaaaaag gcacccgaag gtgcctgagg taaggtgctg aatcgcttaa cgatcgacta
     7261 tcacagaaga ttaatccagc gttggattca tgtgccgtag atcgtatggc gtgatctgat
     7321 agacgtaata gttgagccag ttggtaaaca gcaaattacc gtgactacgc cagctcgctc
     7381 gcggtttgtt ttgcggatcg ttgtgcggga aatagttata cggtacatcc gggtctagtc
     7441 cggcttccac atcgcgtaaa tattcctgcg ccagcgtttg cgcatcatat tcgggatggc
     7501 ccgtcacaaa ggcaatgcgc ttatctttgc tggcaaacag atatgcatcc ccttcttccg
     7561 tctctgccag aatttccaga tcggtgtaat cacgaatcaa cgctgccgga aagtcagcat
     7621 agcgcgaatg cggtgccagg aatgaatcat caaagccacg cgtcagaagc gcatgaggat
     7681 ggagaatatg atgctcgtaa acgccagaga gtttgtcggt gcgagtttgc ttaggaatgc
     7741 cgtagaggat attgagcgcg gcctgtaccg cccagcagac aaacagcgtc gaggtgacgt
     7801 gatctttcga ccactccagc acctgtttga tctgcggcca gtaagcgaca tcattaaact
     7861 ccaccaggcc cagcggcgca ccggttacaa tcagaccgtc aaagttctgt tcctgaatat
     7921 cttcaaagtt acagtagaag ttgttcagat gctctgcggg cgtgttgcgc gattcacggg
     7981 aatcgatgcg caacagctga atatcgacct gcaaaggtga gtttgaaagc aggcgcagaa
     8041 actgattttc agtttcaatc ttcttcggca tcaggttaag gatcagaacc ttaagtggac
     8101 gaatttcctg accagacgca cgagaagttg tcatcacaaa gacgttttct tcacgcaaga
     8161 aattgacggc gggtagctcg tccggcacac gaatcggcat aacctgatta cctcactaca
     8221 tacgcttata cgtttagaca tccagatagc tgaagataac cagaaaattt gccaatgtcg
     8281 agcctgcatg ttgaaggtga gaaagtttca aggaaaaggc tacgttagaa tataagaatg
     8341 acgaaaagga gagaaaatgg ttattagtat tcgccgctca cggcatgagg aaggggagga
     8401 actcgttgcg atttggtgtc gatctgtcga tgccacccac gattttttat cagcagagta
     8461 tcgggccgag ctggaagagc tggtgcgttc ctttctgccg gaagcaccgt tgtgggtcgc
     8521 ggttaatgaa cgggagcagc cggttgggtt tatgttgtta agtgggcagc atatggatgc
     8581 gctgtttatc gatcctgatg tgtgcggctg cggcgtgggt cggatgctgg tggagcatgc
     8641 gctctcgatg gcaccggaac tgacaaccaa cgttaatgag caaaatgagc aggcggttgg
     8701 gttctataag aaggtgggtt ttaaggttac gggacgctct gaggtggacg atttggggaa
     8761 accgtatccg ttgctgaatc tggcgtatgt gggggaataa gccgcctctg ccgtgcctgc
     8821 ccaagctgga gaagacgcgc cagcgtcgca cccggcattt ttttcaccaa
//
LOCUS       DAZSNO010000158         8826 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZSNO010000158 DAZSNO010000000
VERSION     DAZSNO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8826
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.160"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(435..641)
                     /locus_tag="ACHWN2_003415"
     CDS             complement(435..641)
                     /locus_tag="ACHWN2_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR8932820.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="ACHWN2_003416"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="ACHWN2_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR8932821.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="ACHWN2_003417"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="ACHWN2_003417"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR8932822.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4489
                     /gene="kdpB"
                     /locus_tag="ACHWN2_003418"
                     /pseudo
     CDS             2739..4489
                     /gene="kdpB"
                     /locus_tag="ACHWN2_003418"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="ACHWN2_003419"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="ACHWN2_003419"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR8932823.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="ACHWN2_003420"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="ACHWN2_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8722
                     /gene="kdpE"
                     /locus_tag="ACHWN2_003421"
                     /pseudo
     CDS             8037..8722
                     /gene="kdpE"
                     /locus_tag="ACHWN2_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
BASE COUNT         1908 a         2256 c         2514 g         2148 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttattgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8461 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8521 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8581 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8641 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8701 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt
     8761 tattattaaa cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat
     8821 aaaaca
//
LOCUS       DAZSNO010000159         8764 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZSNO010000159 DAZSNO010000000
VERSION     DAZSNO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.161"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..290
                     /locus_tag="ACHWN2_003422"
     CDS             <1..290
                     /locus_tag="ACHWN2_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR8932824.1"
                     /translation="QQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGI
                     SESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
     gene            290..5560
                     /gene="traI"
                     /locus_tag="ACHWN2_003423"
     CDS             290..5560
                     /gene="traI"
                     /locus_tag="ACHWN2_003423"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFR8932825.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRTATSEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5580..6326
                     /gene="traX"
                     /locus_tag="ACHWN2_003424"
     CDS             5580..6326
                     /gene="traX"
                     /locus_tag="ACHWN2_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR8932826.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6381..6941
                     /locus_tag="ACHWN2_003425"
     CDS             6381..6941
                     /locus_tag="ACHWN2_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR8932827.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            7073..7285
                     /locus_tag="ACHWN2_003426"
     CDS             7073..7285
                     /locus_tag="ACHWN2_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR8932828.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            7531..7992
                     /locus_tag="ACHWN2_003427"
     CDS             7531..7992
                     /locus_tag="ACHWN2_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR8932829.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8038..8247
                     /gene="hha"
                     /locus_tag="ACHWN2_003428"
     CDS             8038..8247
                     /gene="hha"
                     /locus_tag="ACHWN2_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR8932830.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            8285..>8764
                     /locus_tag="ACHWN2_003429"
     CDS             8285..>8764
                     /locus_tag="ACHWN2_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR8932831.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     "
BASE COUNT         2073 a         2116 c         2835 g         1740 t
ORIGIN      
        1 cacaacagcc agtgtcttct gtcatcaatg ataagaagtc agatgcaggt gtgagtgttc
       61 cggcaggggg gatcgagcag gagctgaaaa tgaaaccgga agaagagatg gaacagcaac
      121 tgccacccgg gatcagtgaa tccggtgaag tggtggatat ggccgcttat gaggcatggc
      181 aacaggaaaa tcatccggac atccagcagc atatgcagcg tcgtgaagag gtgaacatta
      241 atgtgcaccg ggagcgcggg gaggatgttg agccgggaga tgatttctga tgttgagttt
      301 cagcgttgtt aaatcggcag gcagtgccgg aaactattac accgacaagg ataattacta
      361 tgtgctgggc agcatgggag aacgctgggc cggtcagggg gctgaacagc ttgggctgca
      421 gggcagcgtc gataaggatg tttttacccg tcttctggag ggcaggctgc cggacggggc
      481 ggatttaagc cgcatgcagg atggcagtaa taagcatcgt cccggctacg acctgacctt
      541 ctccgccccc aaaagtgtct ccatgatggc catgctcggg ggggataaac gtcttattga
      601 tgcacataac caggccgtgg attttgctgt ccgtcaggtg gaggcgctgg cttccacacg
      661 ggtgatgacg gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt
      721 taaccatgac accagtcgcg atcaggaccc acagttacac acgcatgtgg tggtggctaa
      781 tgtcacgcag cataacggcg agtggaagac actgagcagt gacaaagtgg ggaaaaccgg
      841 attttccgag aacgtgctcg cgaaccggat tgcgttcggg aaaatatatc agagtgagtt
      901 acgtcagcgc gttgaggcgc tgggttatga aaccgaagtg gtcggtaagc acggtatgtg
      961 ggagatgccg ggcgtaccgg tggaggcctt ttccagtcgt tcacaggcta tcagggaggc
     1021 cgtcggtgaa gacgcctcgc tgaaatcccg ggatgtggcg gcactggata cgcgaaaatc
     1081 caaacagcac gtcgatccgg aaatcagaat ggctgaatgg atgcagacgc tgaaggaaac
     1141 cgggttcgac atccgggcat atcgtgacgc ggcggatcag cgtgcggaga tccgcacgca
     1201 ggcgcccggg cctgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggcgat
     1261 tgccggatta agtgaacgaa aagtgcagtt cacgtatacg gacgtgctgg ccaggacggt
     1321 cggcatactg ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc
     1381 catcagccgt gagcagctta tccccctcga ccgtgagaag gggctgttca catcaggaat
     1441 tcatgtgctc gatgagctgt ccgtccgggc actcagtcgt gacatcatga aacagaaccg
     1501 ggtgaccgta catccggaga aaagtgtccc ccggacggct ggttacagcg atgcggtcag
     1561 tgttctggca caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg
     1621 gcagcgtgag cgggtggctg aactggtcat gatggcccgg gaacaggggc gggaggtgca
     1681 gattatcgct gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg
     1741 tgaactgata accggacgtc gtcagctgct ggaaggcatg gccttcacgc cgggcagtac
     1801 cgttatcgtc gaccagggtg aaaaactctc cctgaaagag acgttaaccc tgctggatgg
     1861 tgccgcacgt cataacgtac aggtcctgat aaccgacagc gggcagcgaa ccggtacagg
     1921 cagtgcgctg atggccatga aggatgccgg ggtgaacaca tatcgctggc agggcggaga
     1981 acagcgaccg gccaccatca tcagtgaacc ggaccgtaat gtccgctatg cccggctggc
     2041 cggagatttt gcggccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggggt
     2101 acgggaacag gccatactga cacaggccat tcgcagtgag ctgaaaacgc agggcgtgct
     2161 cggacaccct gaggtgacca tgactgcact gtcaccggtc tggctggaca gccggagccg
     2221 ttatctgcgg gatatgtacc gaccggggat ggtgatggag cagtggaacc cggagacacg
     2281 cagtcatgac cgctatgtta tcgaccgggt gacggcgcag agtcacagcc tgaccctgcg
     2341 ggatgcgcag ggcgaaacgc aggtggtgcg tatttcctcc ctggacagca gctggtcgct
     2401 gttccggccg gaaaaaatgc cggtggcaga cggcgagcga ctgagggtga cagggaaaat
     2461 tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc
     2521 gatgacggtt gttgtgccgg ggcgggctga accggccacc ctgcctgtgg ctgattcacc
     2581 gttcacggca ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagtga
     2641 cagtgcgacg gtgtttgcct ccgtcacaca gatggcaatg gacaacgcca ccctgaacgg
     2701 tctggcccgc agcggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc
     2761 ggaaaaactc gcccgccatc cgtcctttac ggtggtttct gagcagataa aggcgcgtgc
     2821 cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc
     2881 acagcaggcc attcatctgg cccttcctgt gctggaaagt aaaaacttgg ccttcagcat
     2941 ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa ggaaccggtt ttactgaact
     3001 gggaggggaa atcaatgcgc agataaaacg cggtgactta ctgtatgtgg atgtggcaaa
     3061 aggctatggc acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct
     3121 tcgccatatt ctcgaaggca aggaggcggt cacgccgctg atggagagag tacccggcga
     3181 actcatggag acgttaacgt cgggacagcg tgccgccacc cgtatgatac tggaaacgtc
     3241 cgaccgtttc acggtggtac aggggtatgc cggtgtgggt aagaccactc agttccgggc
     3301 ggtgatgtca gccgtgaaca tgctgccgga gagtgagcgt ccccgggttg tggggctggg
     3361 gcccacgcac cgtgcggtcg gtgagatgcg cagcgccggc gtggatgcac agacactggc
     3421 gtcctttctg catgacacgc agctgcagca gcgcagcgga gaaacgccgg atttcagcaa
     3481 cacgctgttt ctgctcgatg agagctcaat ggtgggcaat accgacatgg cacgggcata
     3541 cgccctgatt gcggccggtg gcggtcgtgc tgtggccagc ggtgacacgg accagctgca
     3601 ggccatcgcg cccggtcagc ctttccgtct ccagcagacg cgcagtgctg ccgatgtggc
     3661 catcatgaag gagattgtgc gtcagacgcc ggaactgcgg gaggcggtat acagcctgat
     3721 taaccgggat gtggaaaggg cactgtccgg gcttgagagt gtgaaaccgt ctcaggtgcc
     3781 acgtctggag ggcgcatggg caccggagca ctccgtgacg gagttcagtc acagccagga
     3841 agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa ggcgaggctt tcccggatat
     3901 ccccatgaca ctgtatgagg ccattgtccg cgactatacc ggcaggacac cggaagcacg
     3961 ggagcagacg ctgattgtca cgcacctgaa tgaggaccgg cgcgtactga acagcatgat
     4021 tcatgatgca cgggaaaagg ccggtgagct gggcaaagag caggtcatgg tgcctgtcct
     4081 gaacacagcg aatatacgtg acggagaact gcgtcgtctc tccacctggg agaaaaaccc
     4141 ggatgccctt gccctggtgg atagtgtgta tcaccggatt gccggtatca gcaaggatga
     4201 cgggctgata accctgcagg atgcggaagg taacacgcgg ctgatttcgc cccgggaggc
     4261 ggtggctgaa ggtgtcacac tgtacacccc ggacaaaatc cgggtgggga ccggcgaccg
     4321 gatgcgcttc acgaagagtg accgggagcg cggttatgtg gccaacagcg tctggacggt
     4381 gacagcggtt tccggtgaca gtgtcacgct gtcggacgga cagcagaccc gggtgattcg
     4441 ccccggtcag gagcgggcag agcaacatat tgacctggcc tatgccatca ctgcccacgg
     4501 tgcgcagggg gcgagtgaaa cctttgccat cgcgcttgaa ggcacggaag gtaaccggaa
     4561 acagatggcc ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca
     4621 ggtgtacacc gataaccgtc agggctggac ggatgccatt aacaatgccg tacagaaagg
     4681 aactgcccac gatgtgctgg agccgaaacc ggaccgggag gtcatgaatg cgcagcggct
     4741 gttcagtacg gcacgggaac tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc
     4801 ggggctggcc gggggagaca gtccggcacg gtttattgca ccggggcgca aatatccgca
     4861 gccgtatgtg gcactgcctg cttttgaccg taacggcaag tccgccggta tctggctgaa
     4921 cccgctgacc acggatgacg gaaacgggct gcggggattc agcggtgaag gacgggtgaa
     4981 aggcagcggg gatgcgcagt ttgtggccct gcagggcagc cgtaacggcg agagcctgct
     5041 ggctgataat atgcaggatg gtgtccggat tgcccgtgat aaccccgaca gcggtgtggt
     5101 ggtgagaatc gccggtgaag gtcgtccgtg gaatcccggt gccatcaccg gtggtcgcgt
     5161 gtggggggat atcccggaca acagcgttca gccgggagcc ggaaatggcg aaccggtcac
     5221 ggcagaggta ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg
     5281 cgcagatgaa attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac
     5341 agagcaggct gtcagggaga ttgccggaca ggagcgtgac cggactgcca cttctgaacg
     5401 ggaagccgcg ctgccggaga gtgtgctgcg tgaatcacaa cgggagcggg aagcggtccg
     5461 ggaggttgcc cgggaaaatc tgctgcagga gcgtctgcag cagatggagc gggatatggt
     5521 tcgtgacctg cagaaagaga aaaccctggg cggagactga tacaggaaga taaacgctga
     5581 tgacaacgga taacacgaac acgacacgta acgattcact ggctgcacgg accgatacct
     5641 ggttgcagtc atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac
     5701 tggtgctgat ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt
     5761 ttctggccgg gcgaggagcc tttccgctgt ttgccctggt gtgggggctg aatctgtccc
     5821 gtcatgcgca tatccggcaa ccggccatta accggctgtg gggatggggg attattgccc
     5881 agttcgcata ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg
     5941 cagtggcggc acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg
     6001 ccattcttct gatggccctg tgggggcctt tgtccggcac cagttacggc attgccgggc
     6061 tgctgatgct ggcagtcagc caccggctgt accgggcgga agacagagcg gaacgtctgg
     6121 cgctggtcgc ctgtctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag
     6181 cggcggtggc tggtcttgtg atgacggtgc tgaccgttgg tctggtgtcg tgtgcaggaa
     6241 aatcattacc ccgtttctgg cccggggatt ttttcccggt attctacgcc tgtcatctgg
     6301 ctgtgctggg cgttctggcg ctgtgacggg tgtggtatct ttgaccgtaa caggatgatt
     6361 cgtcagaggc agaacacagc atgacagagc agaagcgacc ggtactgaca ctgaagcgaa
     6421 aaacggaagg ggagacgcct gtccgcaggc ggaaaaccat catcaatgtc accacgccac
     6481 caaagtggaa ggtgaaaaag cagaagctgg ccgagaaagc agcccgggaa gcggagctgg
     6541 cggcaaaaaa agcgcaggcc agacaggcgc tgtccattta tctgaacctg ccctcgctgg
     6601 atgaggccgt gaacaccctg aaaccctggt ggccgggatt atttgacggt gaaacgcccc
     6661 ggcttctggc ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag cggaatatcc
     6721 cgctctcgca taaaaaactg cgcagggcgc tgaaggccat cacccgttca gaaagctatc
     6781 tgtgtgccat gaaagccggt gcctgccggt atgatacgga agggtatgtg acggagcata
     6841 tttctcagga ggaggaagcg tatgcggcag cgcgtctgga taaaatccgc cgccagaaca
     6901 ggataaaggc agagcttcag gccgtgcttg atgagaaata aaaaaaagcc tccatcagag
     6961 aggaggcagg gaaataaggc ttaaaaggaa ataacttccg tacccgaaag gaagccaatt
     7021 cagtagatta tttaacacca taatactgac gggttaaaga ggagtgataa agatgaatgg
     7081 attcagaaac agttcacgga acggtcaggt ctggcgctgg cagcgtgccg gaagccgggc
     7141 cgttattctg gaggtcagtg gacgctggat ggaagcggca gaagcatggc gacgggctgc
     7201 cggtgttgcg ccccggacag actggcagca gtttgcccga aaaagggctg aacactgcca
     7261 ccggcgcagc cggggaaggg tgtaacgaaa aaagcagcct tcattactcc agggattcag
     7321 aaggctgaat aatgcatgag atttgctttt tttaatatcc ggtcgggcag tatgaccgga
     7381 cagttctgag tagcacagag ttttttccgt attaagtcgt gattttatat tttgtgatta
     7441 atttcacaaa ataaggtgtt attcagtgtg tgctgcgata ttcaggatta cctgaaatac
     7501 aggtgccatt tatctgatat ggagaataac atgaggaaat atattccact ggtattattt
     7561 atcttttcat ggccggtatt gtgtgcagat attcatggac gggttgttcg tgtactggac
     7621 ggtgatacca tcgaggtcat ggactcacgg aaggcagtgc ggatcaggct ggtaaatatt
     7681 gatgcaccgg aaaagaaaca ggattatggc cggtggtcaa cggacatgat gaaatcactg
     7741 gttgcaggaa aaacggttac cgtcacatat tttcagcgag atcgttatgg tcgtattctc
     7801 gggcaggttt atgcaccaga cggaatgaat gttaatcagt ttatggtccg ggccggggct
     7861 gcctgggtat atgagcagta caacacagat cccgtgctgc ctgtccttca gaacgaagcc
     7921 agacaacaaa aacgtgggct ctggtcagat gctgatccgg ttcccccctg gatatggaga
     7981 caccgtaaat aaagcgatgg caatcttatc attcatctgt tctcagcggg aataaaaatg
     8041 gcgaaaacaa aacaggaatg gctgtatcag ttacggcgat gttcatcagt caatacgctg
     8101 gaaaaaatta ttcataaaaa cagagacagt ttattaaaca gtgaacgtga atcatttaat
     8161 tcagcagcag accaccggtt agcggaatta atcaccggaa agttatatga caggatacca
     8221 aaagagatat ggaaatatgt aaggtaataa ttcagaataa ttaattacca ggagcagata
     8281 aacaatgaag ctgattattt ttattctcat tgttctgatt attgcagcgt tacttatccg
     8341 gattatcctc aaaagtgtaa atcagcattc tcctttgctg atgcagttgc atgcggcagg
     8401 tatacggacc ggtgatgcag agcgaatact gtccggcggt gaatactggc aacgtcagaa
     8461 gacgctgctg acagaaaggg aagtcagttt tatgaaagga ctgttcagaa ttgtggatat
     8521 gaagcggtgg tatctgtgtc cgcaggtacg ggtcgcggat atcgtccagc tgaacgggaa
     8581 tatccggcca cgatcacgcc agtggtggca gttattcagg atggtgtctc agtggcatgt
     8641 tgatgtggtt atcgttgaac ggcgttcgtt cagtattgtt gcagcagtag agctggatga
     8701 tgccagccat ttacgaccgg aacgccggcg ccgggatatt cttctggaag aggttctgag
     8761 gcag
//
LOCUS       DAZSNO010000160         8757 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZSNO010000160 DAZSNO010000000
VERSION     DAZSNO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.162"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..436
                     /locus_tag="ACHWN2_003430"
     CDS             <1..436
                     /locus_tag="ACHWN2_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR8932832.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQF
                     AVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="ACHWN2_003431"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="ACHWN2_003431"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR8932833.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="ACHWN2_003432"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="ACHWN2_003432"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR8932834.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="ACHWN2_003433"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="ACHWN2_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR8932835.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="ACHWN2_003434"
     CDS             complement(4438..5145)
                     /locus_tag="ACHWN2_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR8932836.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="ACHWN2_003435"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="ACHWN2_003435"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR8932837.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="ACHWN2_003436"
     CDS             complement(6003..6206)
                     /locus_tag="ACHWN2_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR8932838.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6365..7873
                     /locus_tag="ACHWN2_003437"
                     /pseudo
     CDS             6365..7873
                     /locus_tag="ACHWN2_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7870..>8757
                     /locus_tag="ACHWN2_003438"
     CDS             7870..>8757
                     /locus_tag="ACHWN2_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR8932839.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         2030 a         2387 c         2251 g         2089 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tggcgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct tactcgtgag gatttatgtt ctgctggcag
     6361 tttcgtgatc aacacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt
     6421 gttgtcttgt tcattgtttt tatcgtcggc atggggatgt cggctttccg cacctgaaca
     6481 taatatttac cagaaacagg gaaaaggcgt ggtgtacctg cgcccttata aagaaacaaa
     6541 tctttctcta cctgacgtga attacaagag tttgcgtcgg ttacccaatt tattaataga
     6601 cccgacaaca ctagacgaat gggataaaga accgcctctg actgatttga caaccgacta
     6661 cctctatgag ggcgcacagg cctggtatcc tcattattca tggcatagcg atggtcgcaa
     6721 tatcctctac gcgggtgaag ttgtgcaaaa ccctcccggc acacctcccg tcgatgtcgc
     6781 ctcgttcaag gcatggggtg attttgccgc agataaacac agtctctatt ttgaaggcaa
     6841 gcgcaccgat gacaatggtg gtggaaatag cttggatatc aaaacgctgc atcaggtgga
     6901 attccggcct ccctgggatc ctgacttact ggggctgata ttacgtgacg ctaatttctt
     6961 gtatatcaat gggcatcgcc tcgctgaccc cgagagtttc cgtgtgctgg cgcagaagtc
     7021 ctgggatcag cgaggaaagt tttccaccac gtttaacccc tgcattgccg taccatttgg
     7081 cccctgggat accctggctc gtacacggac caaaatcctg ctcaacggcg aacagcttga
     7141 tgccgacccg gacacctttt ccgtcgtgcg ctggatgccc ggctcactct tgacctggcg
     7201 tgataaaaac gggctgcagc gtaaagtcct cgacaaggaa aatctggcgt gggatgaaga
     7261 tttaacaaag cactgtctgg atttttctct gctggaaaag aaagtgttct ggcgtaaagg
     7321 acctgcttgt aaacaggaag aattacccgg actcgatccg gagcagtttc aacccatcag
     7381 tgatgctgtc gcccagcatc aggactcgct ttataccatc atcgaaacag agtctggtaa
     7441 ccgtaagctg gagatcgtga aacttgatga tcccaatctt attatcaaca aacgtttcaa
     7501 cgccgggaaa cgccacggct atttacttac gcgtgccgaa gggtagccat accattccgg
     7561 tttacacgtg tttgaatctg acggaccgct gatcttactg gataaccgtt ctccggatga
     7621 acgcgaagcc catcttaatg accatccctt tttgcgcaga tggtatgccc gcgataaccg
     7681 ctacatttac agctttgatg gcgcgcagct ctggcgatac cgcaccgctg atccgaaaca
     7741 agttcgctta atctggaagg aacaacattc gggatatgtc tatggcgtaa attacaaaac
     7801 gggatatctg gacggaaaaa ttactgatga cggcgaattt attcctgccc cgcgcaatga
     7861 ggcgacaaaa tgaagggtca aataatcaca gttgcactca tcctgttggg acacacattt
     7921 ctgacgcctg ctgtacaggc catagggttc gactattaca atgatcacgg tgttatgtct
     7981 tacggtaaag gttattgggg ggataagaaa atcattgccc agtttccgga gatgagcagg
     8041 gccggtttgc gccttgtgaa aaatatctct gggaaaacgg gttatatacc ttccgatgaa
     8101 ataagcatag aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt
     8161 ggcaaaatcg ttagcaatcc accgggaaca ctcactgtcg atattgccag ctttcaggct
     8221 atgggccgct tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt
     8281 aatagcggcg cgtcacgtgt tgagctggcg acactaaaag ctatcgaagg taactctacc
     8341 acgctggtgg atagtaaaaa tctctacttg tccggtcgac gtcaggggag cagcagtgat
     8401 gttactgtat tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga
     8461 aattcatatt ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt
     8521 caccttacgg cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg
     8581 gtttttcgcg acaataaggg tctgcatcgt tatccctttg gtcaattatc aggcaaagcg
     8641 atacccgtag atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa
     8701 cagctcacgc ccgaccgtca gtggagcaag tggatagacc taccaggtat tgaacct
//
LOCUS       DAZSNO010000161         8678 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZSNO010000161 DAZSNO010000000
VERSION     DAZSNO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.163"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHWN2_003439"
     CDS             complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHWN2_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR8932840.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            696..914
                     /locus_tag="ACHWN2_003440"
     CDS             696..914
                     /locus_tag="ACHWN2_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR8932841.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            922..1863
                     /gene="ribF"
                     /locus_tag="ACHWN2_003441"
     CDS             922..1863
                     /gene="ribF"
                     /locus_tag="ACHWN2_003441"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR8932842.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1906..4722
                     /gene="ileS"
                     /locus_tag="ACHWN2_003442"
     CDS             1906..4722
                     /gene="ileS"
                     /locus_tag="ACHWN2_003442"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR8932843.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4722..5216
                     /gene="lspA"
                     /locus_tag="ACHWN2_003443"
     CDS             4722..5216
                     /gene="lspA"
                     /locus_tag="ACHWN2_003443"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR8932844.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHWN2_003444"
     CDS             5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHWN2_003444"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR8932845.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5754..6704
                     /gene="ispH"
                     /locus_tag="ACHWN2_003445"
                     /gene_synonym="lytB"
     CDS             5754..6704
                     /gene="ispH"
                     /locus_tag="ACHWN2_003445"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR8932846.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6770..7684
                     /gene="rihC"
                     /locus_tag="ACHWN2_003446"
     CDS             6770..7684
                     /gene="rihC"
                     /locus_tag="ACHWN2_003446"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR8932847.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7851..8672
                     /gene="dapB"
                     /locus_tag="ACHWN2_003447"
     CDS             7851..8672
                     /gene="dapB"
                     /locus_tag="ACHWN2_003447"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR8932848.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         1986 a         2160 c         2461 g         2071 t
ORIGIN      
        1 aggtctaatg aggagatatt tatgaatggt taaagtatat aggaatttaa ttcaaaaata
       61 gagtggtctt aagtttaata cctcaatgtg tatcacaata tccatattct ttgtggggga
      121 gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct
      181 taagccacga agagttcaga tagtacaacg gcatgtctct tttgactatc tggcaaccgg
      241 cagtgtgttc tctcacgcat cacaaaagca gcaggcataa aaaaacccgc ttgcgcgggc
      301 tttttcacaa agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg
      361 ttagccttat gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc
      421 acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata
      481 gctgcgtata ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta
      541 cgagcctttt cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa
      601 ctcccaaatg tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc
      661 ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg
      721 atggcgcagg attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg
      781 cagcaggcgg tcagtttctt cccgtgattt gctccatggc aatgaaaagc cacttctttc
      841 tgatttcggt actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat
      901 tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc
      961 ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc
     1021 gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg
     1081 ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc
     1141 cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg
     1201 cgtttcgaca ggcgttttgc ggcgttaacc gcgcaaaatt tcatcagcga tcttttggtg
     1261 aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt
     1321 gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga tatcaccagt
     1381 acgcaaactt tttgcgaagg tggtgtgcgt atcagcagca ccgccgtgcg tcaggcgctg
     1441 gctgatgaca atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg
     1501 cgtgtagtcc acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg
     1561 ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt
     1621 gaaaagccgt tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc
     1681 cagcagctgg aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa
     1741 gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa
     1801 gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct
     1861 taagcctgtt atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa
     1921 tcaaccctga atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa
     1981 ccgggaatgc tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa
     2041 aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat
     2101 attggtcact cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc
     2161 ggttatgact cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa
     2221 gtagagcaag aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag
     2281 tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc
     2341 gtgctgggcg actggtcgca cccgtatctg actatggact tcaaaactga agccaacatc
     2401 atccgcgcgc tgggcaaaat catcggcaat ggtcacctgc acaaaggcgc gaagccggtg
     2461 cactggtgcg tagactgccg ttctgcactg gcagaagcgg aagttgagta ttacgacaaa
     2521 acttctccgt ccattgacgt cgctttccag gcggtcgatc aggatgcgct gaaagcgaaa
     2581 tttggcgtaa gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg
     2641 actctgcctg cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag
     2701 atcgacggtc aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc
     2761 ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg cagagcttga gctgctgcgc
     2821 tttacccatc cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg
     2881 gatgccggta ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc
     2941 ggtcagaaat acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg
     3001 ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg
     3061 ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc
     3121 tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacac cgcagtggtt cgtcagcatg
     3181 gatcagaaag gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggctcccg
     3241 gactggggcc aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc
     3301 cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg
     3361 catccgcgta ccctcgaact gatggaagaa gtggctaaac gcgttgaagt tgacggcatc
     3421 caggcgtggt gggatctcga tgcgaaagag atcctcggcg acgaagctga ccagtatgtg
     3481 aaagtgccgg atactctcga cgtatggttt gactccggat ctacccactc ttctgttgtt
     3541 gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag
     3601 caccgtggtt ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg
     3661 tatcgtcagg tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa
     3721 tccatcggca acaccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg
     3781 cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg
     3841 aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac
     3901 ctgaatggtt ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat
     3961 cgctgggccg taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca
     4021 tacgatttcc acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc
     4081 ttctacctcg acatcatcaa agaccgtcag tacaccgcca aagcggatag cgtggcgcgt
     4141 cgtagctgcc agactgcgct gtatcacatc gctgaagcgc tggtgcgctg gatggcacca
     4201 atcctctcct tcaccgctga tgaagtgtgg ggctatctgc cgggcgaacg tgaaaaatac
     4261 gtcttcaccg gtgagtggta cgaaggcctg tttggtctgg cagacagtga agcaatgaac
     4321 gatgcgttct gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa
     4381 gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa
     4441 ccggaactgg cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc
     4501 tccggcgcta ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta
     4561 ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg
     4621 cactacaccc aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc
     4681 agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt
     4741 caacagggct acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca
     4801 aatacctgat cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta
     4861 atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct
     4921 ggcagcgttg gttctttgcc ggtattgcga ttggtattag cgtgctcctg gcagtgatga
     4981 tgtatcgctc gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg
     5041 gcgcgctggg caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact
     5101 tctacgtcgg cgactggcac ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg
     5161 gtgcggcact gatcgtgctg gaagggtttt taccttccaa agcgaaaaaa caataataaa
     5221 tcctgccgga tgcggcctac agattgctac gaaatcgtag gccgcatccg gcaaaaatcc
     5281 tcaaaaataa gagcaaacct gcatgtctga atctgtacag agcaatagcg ccgtcctggt
     5341 gcacttcacg ctaaaactcg acgatggcac caccgccgag tctactcgca acaacggtaa
     5401 accggcgctg ttccgcctgg gcgatgcttc tctttctgaa gggctggagc aacacctgct
     5461 ggggctgaaa gtgggcgaca aaaccacctt ctcgctggag ccagatgcgg cgtttggcgt
     5521 gccgtcaccg gacctgattc agtacttctc ccgccgtgaa tttatggatg caggcgagcc
     5581 agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc ctggtgtgat
     5641 ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg ccgggcagac
     5701 cgttcatttt gatattgaag tgctggaaat cgatccggca ctggaggcgt aacatgcaga
     5761 tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc agcattgttg
     5821 aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg gtgcataacc
     5881 gctacgtggt cgatagcttg cgcgagcgtg gggctatctt tattgagcag attagcgaag
     5941 tgccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg gttcgtaacg
     6001 aagcgaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg accaaagtgc
     6061 atatggaagt tgcccgcgcc agtcgccgtg gcgaagaatc tattctcatc ggccacgccg
     6121 ggcacccgga agtggaaggg acgatggggc agtacagcaa ccccgaaggg ggaatgtatc
     6181 tagtcgaatc gccagacgat gtgtggaaac tgacggtcaa aaacgaagag aagctctcct
     6241 ttatgaccca aaccacgctg tcggtggatg acacgtctga tgtgatcgac gcgctgcgta
     6301 aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc acgactaacc
     6361 gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg gtcggttcga
     6421 aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc aaacacgcgt
     6481 ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt aaatgcgtcg
     6541 gcgtgactgc gggcgcatcg gctccggata ttctggtgca gaatgtggtg gcacgtttgc
     6601 agcagttggg tggtggtgaa gccattccgc tggaaggccg tgaagaaaat attgttttcg
     6661 aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt agcagcctaa
     6721 gttatgcgaa aatgccggtc ttgttaccgg cattttttat gaagaaaaca tgcgtttacc
     6781 tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg ccgcgatttt
     6841 tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct cggttgagaa
     6901 aactacccgc aatgccctgc aactgctgca tttctggaat gcggagattc cgctcgccca
     6961 gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc acggcgaatc
     7021 gggaatggct ggctacgact ttgttgagca caaccgaaag ccactcggga taccggcgtt
     7081 tctggcgatt cgggatgccc tgatgcgtgc accagagccc gttaccctgg tggccatcgg
     7141 cccgttaacc aatattgcgc tgttactttt acaatgcccg gaatgtaaac cgtatattcg
     7201 tcgtctggtg atcatgggcg gttctgctgg tcgcggcaac tgtacaccaa atgccgagtt
     7261 taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggca tcgaaattgt
     7321 catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc tcgctacttt
     7381 accggagttg aatcgcaccg gaaaaatgct ccacgccctg tttagccact accgtagcgg
     7441 cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc tggtgcgccc
     7501 ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg aatttacctc
     7561 aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg tacaggtggc
     7621 attggatctg aatgtaaaag gcttccaaca gtgggtggct gaggtgctgg ctctggtgcc
     7681 gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt tcactgatat
     7741 ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg cgtagcgatg
     7801 cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct atgcatgatg
     7861 caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag ttgattcagg
     7921 cggcactggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa ggatcttctt
     7981 tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt accgtgcaaa
     8041 gcagtcttga tgcgataaaa gatgattttg atgtgtttat cgattttacc cgtccggaag
     8101 gtacgctgaa ccatctcgct ttttgtcggc agcatggcaa agggatggtg atcggcacta
     8161 cggggtttga cgaagccggt aaacaggcaa ttcgtgacgc cgccgccgat attgcgattg
     8221 tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg gagaaagcag
     8281 ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat agacataaag
     8341 ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac gcccttgata
     8401 aagatctgaa agattgcgcg gtctacagtc gtgaaggcca taccggtgaa cgtgtgccag
     8461 gtaccattgg ttttgctacc gtgcgtgcag gtgacatcgt tggcgaacat accgcgatgt
     8521 ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgc atgacatttg
     8581 ctaacggtgc ggtaagatcg gctttgtggt tgagtggtaa ggaaagtggt ctttttgata
     8641 tgcgagatgt gcttgatctc aataatttgt aaccacaa
//
LOCUS       DAZSNO010000162         8559 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZSNO010000162 DAZSNO010000000
VERSION     DAZSNO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.164"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..528)
                     /locus_tag="ACHWN2_003448"
                     /pseudo
     CDS             complement(1..528)
                     /locus_tag="ACHWN2_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(579..1154)
                     /locus_tag="ACHWN2_003449"
                     /pseudo
     CDS             complement(579..1154)
                     /locus_tag="ACHWN2_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1158..1558
                     /locus_tag="ACHWN2_003450"
                     /pseudo
     CDS             1158..1558
                     /locus_tag="ACHWN2_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1961..2542)
                     /locus_tag="ACHWN2_003451"
     CDS             complement(1961..2542)
                     /locus_tag="ACHWN2_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932849.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(2589..6446)
                     /gene="sigA"
                     /locus_tag="ACHWN2_003452"
     CDS             complement(2589..6446)
                     /gene="sigA"
                     /locus_tag="ACHWN2_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR8932850.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(6807..7811)
                     /locus_tag="ACHWN2_003453"
                     /pseudo
     CDS             complement(6807..7811)
                     /locus_tag="ACHWN2_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            7796..8173
                     /locus_tag="ACHWN2_003454"
                     /pseudo
     CDS             7796..8173
                     /locus_tag="ACHWN2_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8212..>8559)
                     /locus_tag="ACHWN2_003455"
     CDS             complement(8212..>8559)
                     /locus_tag="ACHWN2_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8932851.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         2083 a         2028 c         1977 g         2471 t
ORIGIN      
        1 tgctgaacat ccccggactt ctgcctcgat ggtatacagt tcaccgatac gctgcagggc
       61 ttccgtggtg atgtcggttg gcactcttgc atgcacatcg tggatttttc tccgggcatg
      121 ggccatacac gcagcttccg ttattctgcc ggattcgtat aacgcccggt aaccaccgta
      181 agcatctgcc tgaagcacac cgctgtaacc ggctaggtga ttctgtggat ggataccttt
      241 ccggtccggg ctgtacgcga accagaccgc cgggggcatc tgtgaaccgg cgttacggtc
      301 atcacggaca tagacccaca gccgggcagt tcttgtttta ccactgcccg gctcctggac
      361 cggaacgggg atatcatccg catgaacttt accgggcatc agtacatact ggcgcaggac
      421 gtcatacagc ggctccagca gttcagcaac ggcaccggtc cagcgaccca gtgtggcacg
      481 gctcagctcc acgccctgac gacggtatat ttctgactgg cggtataact gtcaacgacg
      541 gatgaaaagt ggctcttccg caacctgggt ggagattttc atgccagtgg actcattacc
      601 ctctgcctga gtagttcaaa tcctgctcga ccatacatct ggcgtttcag catcttcttc
      661 aggcgattta catgaccttc gacaacacca ttactccagc gactactgat tgcctcacat
      721 atagctgcag catcagcttc catccccgcc gccacgcgcc gaaattctgc tgaaccgctt
      781 tcgtggactc gagtgaacca actgcttagc tgtgatttat tttgggtttt cagaatacgg
      841 tagaactcga gtgccagttg ctgcgctatt ttcagctcag gttctttttc acacatcaga
      901 ctaataaatc gggaagcata attttcttca tccctggtta ttctccaggg catcaaccag
      961 cggcttaccc gggagactga aggaagtctg acggcagtag taaccggtgg gttccagcct
     1021 ttacgccatt tggcaaccgc atccctgacg atggtttcac tgcctgtaaa tccccgggcc
     1081 accatttccc gccatatccg gctggcgtta taattaccac tttctcgttg ctcttttaac
     1141 cactccctcc atggtgtcag cgaaaaacga aaccgctgtt gaaatccctg gaaagctggt
     1201 tgcggaaaag atgaagaccc agtcgcgaca ctcagagttg gcgaaggcgt tcgcgtacgc
     1261 acttaaccag tggccggcac tgacgtacta tgcgaacgat ggctgggtgg aaatcgacaa
     1321 caacatcgct gaaaatgcct tgcgggcggt cagtctgggt cgtaaaaact tcctgttctt
     1381 cggctctgac catggtggtg agcggggagc gctactgtac agcctgatcg ggacgtgcaa
     1441 actgaatgac gtggatccag aaagctacct tcgccatgtg cttggcgtca tagcagactg
     1501 gccggtcaac cgggtcagcg aactgcttcc gtggcgcata gcactgccag ctgaataaca
     1561 catccccgtc aatacggccc tcgctgtacg cttactcatc acctgctatt aatccccgag
     1621 tcagtacttc gcggcctgac gcgatcaccc tactcagtac ttcagagccc gcctccggta
     1681 cctgcctggc gatcgcccct gtgcaacaaa ataaatcaac cgttatcaaa ccacgatcgt
     1741 tcgtatgcgc cgccgatgac gcgcgcctga aaaacgcgtc aatatgagtt ccccggaagc
     1801 ttacgataat acgcttatga acattacaac acgctcatgc ggaacctgga tgggggaagg
     1861 aacggtgaag ctgttagcga atgaccggcg tttatacaaa cgccggtcat gatgaatgat
     1921 atttcagaat atacctcggg ctaaaatacc gggacagaga tcaggcgtga ttaatctgca
     1981 gcatttttaa taacagaagt actgagctta ctttaccttt gatgatgtaa tagttatttc
     2041 cgtcggtact catttttccc tgaaataatg tcacggatgt ttcttcctgc tgatcctgca
     2101 ggcgcagtcg taaggtcata tttttaatga gctgagatcc gggtggcaag gggtatttgc
     2161 tgtcagatac tgggcgactg agtctgatgt ggcgcgtata cagcccgttt tcgtacaggg
     2221 tggcctctgt tatggtgtca gtgaccagca tgtgggagac aatgctgtat tcttttttaa
     2281 agcacccacc agaaacggtg attgtaatga taatggtctt ctcgatcgag atgtaaatac
     2341 ccgtttcgtt atactgtccg tatcggaacc atgagaactc agttgcattc cagccatgct
     2401 ccagaatatg tgcatttttt tgcctgatag tttctttttt tattcgctca tgtttaaggc
     2461 ataacaggca gataaataat gtcagaaaaa gaaagctaat aatgagagca agagcgatat
     2521 agtctgtcac agggtaatac atattctctc cggtagaaga agggccgcaa acgcggcccg
     2581 ggctgttatc agaaagagta acggaagttg gcgttgatcg cgttatccac gttgtattta
     2641 ccaaatgccg atttctcaaa ctcaagaccg aagcgaagat tatcgcgaat ttcggcgttg
     2701 agaccaacat tcatgagaat acgaccgtct ttttcacctt tgatacgttt ctcaccggac
     2761 gcatcacgca gtacggtttc accgttggca aacaggtcaa actggtagcc aaggccggcg
     2821 cgggctgtga ctttccagtc cttaccggag aaggatttac ccacatcaac accggtacgc
     2881 ccaatcagcg gattaaagtc cttatccttc atggtcacgc tcattccgcg atcctgccag
     2941 tcgaaccgtt tacctgatac ggccccataa accagttctg cctgcggctc aatccaggag
     3001 tcttctgtta catgatagcg gtagcctgct tcagcaccca catacaagga atgagaactg
     3061 tagtcttttg ttccgagtcc agcaaaggtc gccgagtact cattatcatg gtgaacatac
     3121 ttactaatca ggtcgatata ggcaccagag tcaaatatag cggaagcata cagacctgcc
     3181 ccgacggact tcgtcttgcc actgaatgca ttactgctgg catgactgtc ggtatacgtc
     3241 atagtcagac cagtgaaaag gtcaagtcca tccagctcat gttttttatc cacaccaatc
     3301 tgcacatggg tgtagttgtc actgtatcca ccagaagctg aacctgctcc gctcatgatg
     3361 cgggcccatg caccggcctc gccattggtg tcacgcagat cccccattcg tttattcagg
     3421 ttgttgactt ctgtcaggaa ggctttgtaa ttaccggaca tatacgattt tgctgcaccg
     3481 gaagatgctg tattttcagc caccttatat ccggtcaggg tccacgtggt atgttcctga
     3541 tcctctttcg tgctgatcac aggagatatg ttgctgaaac ctatggcctg agtactggct
     3601 ttaaatatct ctttattgct tccttttggg gctgttatca ggtcaacgtt taatcctgag
     3661 ttattatttc ccactttctg tatatcgacc gacagactgt tattgcttcc ggttaactga
     3721 tttttaacaa tgagtttatc cgattgattt acagactggc tcagatcagc ccgcataatc
     3781 acagcagtat tagtggcatc cagtgtgttt actgtcacag tatggaaacc cgaattctct
     3841 atgttactaa acaacaaaag actgtcgcgg gtttttaatg attgcagggc tgactgtccg
     3901 tttactctcc ataaggtgtt atacatgctg gctgaggctg caggagcatc aatacctccg
     3961 gtataagaag tatcaaagcc acccagcaaa cctgtagcca gcggtgtgtg tgtggttttg
     4021 tccaggttat cttctttata ctggtcaaca ccaaacagaa tagatgcttt tttgtctgat
     4081 tttatattac cattgaccca ggcataattt tgagccttta acgtggcacc atcttctgtc
     4141 atattatatc catctgacat atagattgcc gggctgtatt tattattttc tgtaggggtg
     4201 cctgttaatg tcagggttcc ggccagttca atagctttat ctacatacat ttttccctgg
     4261 gcggtaacag tagctccttt gttgataatc aggtctgtat taattaagga taatgcacca
     4321 gtcatagtaa cagttgattt atcaccaaca gttattttgc tgttttgagc actcagactg
     4381 ccgttgaaac tactttcgcc tgctgaatcc ccggagcgaa gctgttgtct gaatccaaaa
     4441 ccttcgccag taatgtttct tcctgagaac atgtcaatgt atgcagtttt atttcccaga
     4501 acaatttgag agtttttagc ctctatgtgt ccttttacat tagcatcccg gcctatactc
     4561 agggttgcgt tttctaaatt taatttccgg aaattaaatt tacgactttc ccagtcgggc
     4621 tgggaaaaag aggctatctg attgttggac ttatattccg tattgtaacg tttatttaca
     4681 gtgctttcct ggtcctttat ggcagcagaa tagtcttttt gacataaaaa aggaattgga
     4741 cagttgttat tgccttcttt aaatatggca tgttctgtcg catgtccttg taatgttaac
     4801 tgtgcattcc ggacagagat atcgtttttg atatcgactg agccatcaaa gatcagattg
     4861 gcattattgt tatgttgctg ggtagtattg ataatatgat ttatgcttat attacctgat
     4921 acattcccat ggtacatata tgcatcagta ttagtaagtg ataatgttga ttgcttcacg
     4981 tttgagttcg ttacggttgt tcccgcatct gttgctgcga ttttctgaaa tgactggtca
     5041 tagccgttta gatccagtgt tccgccattt ctggtaaaat agatttcgcc attaccgctt
     5101 tcgcttaaag cgtctttggc atttattttt accgtacctt tacctccggc catgtaaatt
     5161 ttattgaagg ctttttcagc actaaggatg acagtcccat tacctatttt aaggttattt
     5221 ccctgtgcta tttttacatc cagagtacca ctacctattt tatgcagatt atctccggca
     5281 acgcccttaa cattccattc aacggtggta ttattatcaa tatctatccc ggcaccttta
     5341 taagacttat cttttccgta cactttatat gtctggttct tatcgaatat aagcccgcca
     5401 atgccaagat cggtatcctg cttgagcgtt aatacccctc ctcctgagac agacaggtcc
     5461 ttatctttta taatcgatga taactcttgt tttttaccat ccagctgcat agtgtttgtg
     5521 aaattctttt ttaaattgtt taccagaata ttattaaata acgagtagtt tgtccaggct
     5581 atattggtac tactgataaa accaactgta ctggtaacac catggaaaac ccatttttgt
     5641 tgttctttat catatagata agaaccagaa ccactatcgc caaaaattgg tgagttttta
     5701 aaaggggttc tgttggtata aatgtaactc ttgtttttcc agtccagatt aaaaagactt
     5761 gcgcttaaca agtcgggatt atagttttgt ccaaacagat attgtttgcc gtttaatatc
     5821 gttgaggttg ttccggcacc tgctctgaag ccaattattt gttccttacc gttgtagttt
     5881 acgccatatc ttttcatcgc atcttcggga gaaagtgaga agtcggcact ctccgtaacc
     5941 cctgttgttt ccacgacgaa tttattcaga cgatgaaccg aaaagtcgcc agatgatact
     6001 ctgttacttg ctttatatgc tgtctgatcc catgactgtg ttgttattgc atgatgctgt
     6061 gtaccgttat gagtcgcagt aacactgtac gcacctccaa ttgatgttgt tgcacctttg
     6121 ttggaaacag cagaaaaatc aggtacaggt agttctggaa aatgaaatat ctttccatct
     6181 ttcatcatta attgaacatt tgttgcaccc ggtctgaact ctcctctgtt ttgagcaagg
     6241 tcaagataat cgcgggtcca tatattattt atatccagca ttgctgcaaa tgctgtttcg
     6301 ccataacttc catatattga ggataaaata ataccgagga taactttctt tcttgatgta
     6361 ccgacactaa ctttccgggt cagttcagaa acagcaacta atccacctgt aatatgacta
     6421 tatttcagtg aataaatttt attcatacgt gaactccgat atttctgagt tgtggttatg
     6481 ggatagcatt taattacaac ggggaactat cagtcatggt acgtaactga taagggtata
     6541 atgataatca ctgtcagtta tatttgtatg acagatgtaa caaaatgaaa aaaaagataa
     6601 gcacaagtga ttgattggtt agatattgat cacttggtgt ttgtattttg aattttgcga
     6661 tcaaaatttg atctttgttg tttccggtca atcctttaca ggttttctgt attatccggg
     6721 ggagttaagt ctgtcgatgt ccgacagact tttgatgatg gaaacaacat gcagcgataa
     6781 tgtgcatgct gttttgcagg tggaactcag aaggtgaact ggaactgtgc gaccgcaccg
     6841 taagtgttgt ccgtgccgat caggcctgca acatcaaggt gaaccacatc aaacggtgag
     6901 atccccaccc cggccgtgaa tgcactgccg tcgttaccgg ccagattctg acggtaaccg
     6961 gcgcgcagct gtgcccattt ccaggcatta aattctgtgc caatggcggc aaactgacgg
     7021 ttgctgtcgg aggtgaaacc gctggccggg gtcagatcca catcaaatgc agtggtgaac
     7081 atcgcgttgt gccaggagac accggccgtc acctgcgggc ggatcctgaa ggtttccgtg
     7141 ataccgttca cttcttttgt ctcaatacta cgggagatca ggttctgtgc gaccagtccc
     7201 agcgtccagt tgtcatccag gtccgtactg gcaccgatgt cggcgttgat gccatttttc
     7261 gtgttgtggt agcggtcgcc tttgaaggcg ctgctgtcat aatttctgac cagcacgttg
     7321 tagttgaaca ggtcgacgcg ctggaatttg ggggggaagc ccagagacca tttctgcccg
     7381 gccgtttcca gttccgtagc cagagcaata cccacatcag tgatcagtgc cgtgcgggca
     7441 caggcctgtg atgtcaggcg gcttttgtcc acctcctgcc cggtgctgtt tgccacctct
     7501 tccaggtaag tgaggtcagc atcagagact ttaccgtcga cactgacggt gccgtgtgat
     7561 ttaaccatca gtgcggcaga gaggcgatca ccgggaaggg ccgccacagc agaaacgccc
     7621 agctgtgcgg cggcatgtgt gttacggaag tcccggaggt gttctttcag gcgtgcggca
     7681 gcctcaggaa caccgtggtt actttcgatg gcgcggtcga aggccttcca ctcatcactg
     7741 atccggtcgg cgttgtcggt gatattatcc ggattggaga gctgcgcgcc gacggacgga
     7801 agcaggatgc tgggctcatg cgcgacgcaa aatccacgat gtatatatca gtactaaaag
     7861 cgcgacggcg gaagaagctc tgaaactaat cggtgaactg tacgccatag agcacgatat
     7921 acgcgggtta ccggtgtctg aacgcctggc ggtcaggcaa atgcagagta gaccgctcct
     7981 gacttccctg tataagctga tgcaggagaa agaacacacg ttatcgaaga aatgtcgtct
     8041 gggagatgcg ttccggtata tcaggaagca ctgggctgcg ttgtgcaact tctgtgatga
     8101 tggcctggct gaggtagata ataatgcagc agaaagagcg cttcgtgcag tctgtctcgg
     8161 gaagaaaact ttttcgagga tcactcattc cgtgtctgac tgcttcaggg tttaattttg
     8221 cttctgctga ctgaatgatg attgggcagc aagtgcaata tcccacataa atccagtcaa
     8281 ctcacgagcc accgccgtaa tggctacttg tgattttttc ccttttgctg tcagagttcg
     8341 gtaacgagaa cacaatcgtt gttgagcttt ccatgcaata tctttggaat attgtgttac
     8401 agagtccggt cggtaatata taagccatgc tccaactttt gcaggtgtac gataagacca
     8461 ggcggcttcg taaagcagac gtctcagttc gctgtttcca acttttgtta ttcctctggg
     8521 acgaatactg tttccactgg aatattcacc tggcaccag
//
LOCUS       DAZSNO010000163         8494 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZSNO010000163 DAZSNO010000000
VERSION     DAZSNO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.165"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            44..397
                     /locus_tag="ACHWN2_003456"
     CDS             44..397
                     /locus_tag="ACHWN2_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR8932852.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACHWN2_003457"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACHWN2_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFR8932853.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACHWN2_003458"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACHWN2_003458"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR8932854.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACHWN2_003459"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACHWN2_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR8932855.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACHWN2_003460"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACHWN2_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR8932856.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACHWN2_003461"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACHWN2_003461"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR8932857.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACHWN2_003462"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACHWN2_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR8932858.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6415..6876
                     /gene="hycA"
                     /locus_tag="ACHWN2_003463"
     CDS             6415..6876
                     /gene="hycA"
                     /locus_tag="ACHWN2_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR8932859.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7001..7612
                     /gene="hycB"
                     /locus_tag="ACHWN2_003464"
     CDS             7001..7612
                     /gene="hycB"
                     /locus_tag="ACHWN2_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR8932860.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7609..8494
                     /locus_tag="ACHWN2_003465"
                     /pseudo
     CDS             7609..8494
                     /locus_tag="ACHWN2_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT         1903 a         2307 c         2287 g         1997 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgttg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gccgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agtaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 gttggcacaa aaaatgctta aagctggcat ctctgttaaa cgagtaacct gacaatgact
     6421 atttgggaaa taagcgagaa agccgattac atcgcacagc ggcatcgtcg cctacaggac
     6481 cagtggcaca tctactgcaa ttcgctggtt caggggatca cgttatcgaa agcgcgcctg
     6541 catcacgcca tgagctgcgc gccggacaaa gaactctgtt tcgtcctttt tgaacatttt
     6601 cgtatttacg tcaccctggc ggatggcttt aacagccaca ccatcgagta ttacgtcgaa
     6661 acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc aactgagcat tgacggcatg
     6721 attgatggca aggtcaacat ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa
     6781 atcgctggcg tttacgacag cttatacacc gccattgaaa acaatgtgcc ggtgaattta
     6841 agccaactgg taaagggaca aagcccggca gcatgagctg aggctttgcc cgttttgcag
     6901 gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc
     6961 atgttttcgt caaaaatgac aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc
     7021 cgactccacg ctctgtatcg gctgccacac ctgtgaggcc gcctgttcag agacgcatcg
     7081 ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg ctcaatgaaa aagaatctgc
     7141 gccgcagctc tgtcaccact gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc
     7201 catcacccgc gttgatgggg ccgtgcagtt gaatgaaagt ctgtgcgtaa gctgcaaact
     7261 gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc ggcagccgtc cgctggatat
     7321 tccggcaaac gccaataccc cgaaagcgcc accggcaccg cctgctccgg cgcgtgtcag
     7381 cacattgctt gactgggtgc caggtattcg cgcgatcgcc gtcaaatgtg acctttgtag
     7441 ctttgatgaa caaggtccgg cctgtgtgcg gatgtgcccg actaaagccc tgcatctggt
     7501 ggataacacc gatatcgccc gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga
     7561 ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt
     7621 tccctgatca atagcggcgt ggcatggttt gtcgccgccg ctgttctggc atttctcttt
     7681 tcaaaaagcg ctaagtggct ggatagccgg aattggcggc gcggttggta gtctgtatac
     7741 ggcagccgcg ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct
     7801 ggtaagctac gatgtgcaaa tctccccgct taacgcgatt tggctgatta ccctcggcct
     7861 ttgcggtctg tttgtcagcc tctataacat tgactggcat cgccacgcgc aggtgaagtg
     7921 caacggcttg cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa
     7981 cctcggcatg ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag
     8041 caacagcaaa gagggcaaac tgtggtttgc gctgaggcgt cttggcactc tgctgctggt
     8101 gattgcttgc tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tgctggatat
     8161 gcgtatgcaa cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
     8221 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
     8281 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
     8341 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
     8401 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
     8461 cctgctggct taccacaccc tggaaaatat cggc
//
LOCUS       DAZSNO010000164         8397 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZSNO010000164 DAZSNO010000000
VERSION     DAZSNO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8397
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.166"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(489..1685)
                     /gene="hemY"
                     /locus_tag="ACHWN2_003466"
     CDS             complement(489..1685)
                     /gene="hemY"
                     /locus_tag="ACHWN2_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR8932861.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1688..2935)
                     /gene="hemX"
                     /locus_tag="ACHWN2_003467"
     CDS             complement(1688..2935)
                     /gene="hemX"
                     /locus_tag="ACHWN2_003467"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR8932862.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPAPQGDTPAAAPQGE"
     gene            complement(2957..3697)
                     /gene="hemD"
                     /locus_tag="ACHWN2_003468"
     CDS             complement(2957..3697)
                     /gene="hemD"
                     /locus_tag="ACHWN2_003468"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR8932863.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3694..4635)
                     /gene="hemC"
                     /locus_tag="ACHWN2_003469"
     CDS             complement(3694..4635)
                     /gene="hemC"
                     /locus_tag="ACHWN2_003469"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR8932864.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5022..7568
                     /gene="cyaA"
                     /locus_tag="ACHWN2_003470"
     CDS             5022..7568
                     /gene="cyaA"
                     /locus_tag="ACHWN2_003470"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR8932865.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7608..7928)
                     /gene="cyaY"
                     /locus_tag="ACHWN2_003471"
     CDS             complement(7608..7928)
                     /gene="cyaY"
                     /locus_tag="ACHWN2_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR8932866.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7976..>8397)
                     /locus_tag="ACHWN2_003472"
     CDS             complement(7976..>8397)
                     /locus_tag="ACHWN2_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR8932867.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1878 a         2191 c         2176 g         2152 t
ORIGIN      
        1 caggtttatt atatcgcgtt gattattgat gctgttttta gttttaacgg caattaatat
       61 atgtgttatt aattgaatga attttatcat tcataataag tatgtgtagg atcaagctca
      121 ggttaaatat tcactcagga agttattact caggaagcaa agaggattac agaattatct
      181 cataacaagt gttaagggat gttatttccc gattctctgt ggcataataa acgagtagat
      241 gctcattcca tctcttatgt tcgccttagt gcctcataaa ctccggaatg acgcagagcc
      301 gtttacggtg cttatcgtcc actgacagat gtcgcttatg cctcatcaga caccatggac
      361 acaacgttga gtgaagcacc cacttgttgt catacagacc tgttttaacg cctgctccgt
      421 aataagagca ggcgtttttt tatgcatcag aaaggtcccg gaggtgcttt cctccgggtg
      481 agaaggaact actgtggcgg gttattctgc aacgttaaca tcaaaccgtc gcgacgcatc
      541 gctgcagctt cttccggctt gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg
      601 taggcgtccg gacgttgttt cagcgccgcg cggaaggcga gcgatgcttc ctgccattca
      661 ccgtgcttca tcagtgactg acccaatgtg ctccacaaca gcgggcgatc accgacgttt
      721 ttgatttgct ggcgtagcac tttttccagc tgttccgggt tattggtttt cagacgcggg
      781 atcggcagca gtaggcgatc gtcatactgg cgtttcaggc cgtcgatgat aatttgctgg
      841 gcagtatcat gatcgtcaca ttcaataaga tgttccgcca ttgccacctg caacgctacc
      901 tgatgacgcg ttttccggct ttggtttttc caccagttac gcaaaccttc actaccgtta
      961 tcggcacgcg cctgatccat caggccaatc catgcctgtt gttccagcat tgcacgatgt
     1021 tcttcatcac caacatgggc tttcgccatt gatgggatga tatccagcag agaactccat
     1081 gcccctgtgc ggatatacgc ctgttccgcc agacgtaata cttcaggatg gcgtggcgta
     1141 acttccagca gcttatccac gccgtggcgt gcagcatggt tttcattacg ggccagttgc
     1201 agacgtacac gggtgatttc taccggaatg gtgtcattgc cggccagctc cgctgcgcgt
     1261 tccagatgtt ggttggcgcg tgcttcatca ccacgttgtt gcgcggcttc ggcagccagt
     1321 agatagttca ccaccggttg ttcagcgtga tcggcatttt tcgccatcag cttttcaact
     1381 tgctgataat caccttccgc cagtttcagc agcgcctgtt cggtctgctt acgcgcacgg
     1441 cgacgcttac gtccgacaaa ccatccacgg gtgtgcgcgc cagtgcggaa gatccgccgc
     1501 agtagccact caatggcaaa cagcactacc atcgccagaa tcaatatgat cgccaggccc
     1561 gtgacgctgg tttcgatatt gtagttgtcg gtctggatca gcacataacc ctgatgaccg
     1621 gcaatcatcg ggccaaccac tatccccgca atcagcaaaa caaagagtaa taacactttt
     1681 agcatggtta ttctccttgc ggcgcggctg ccggagtatc accttgcggt gcaggtgcag
     1741 gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag
     1801 gtgcaggttt agcttccgtt gtccccgctg ccggttgtgc cagcaggtta cgcacgcgag
     1861 tctgcatcag tttttccagc atcgcctggc tttgcagggt ttccggaaga tccatcgaga
     1921 tattttgctg gcttaactgg tccacctcgt cgaggaacgc tttggtggtg gcatcatcag
     1981 tatcgtagta agcacgtacc caggtggaga cgttctccag cgcctggcga taagtctctt
     2041 cctgatgacg cggtacagct tgtgctgcga ccagcaggcg agagcgaata ttttcgcgca
     2101 gatagatatc ctgatttggc gctaacagcg gtacggcggt gtcatcacga cggcgaatcg
     2161 taatgaagtt gtccataaag ttctgccagc ttttttgcag attgatacgc cattcgctga
     2221 tggaactgga aagctcttca ccgtctgaat ccatcggcga accatcgctg tcattatcgg
     2281 ccagacgcag gttatctacc tgatttgaaa gctgattaag cttaaggatg atgccgtcat
     2341 aatccacctg cgatactgca gaaaggctgg cgatatcatc ggtaattgcc cgacgaacgg
     2401 taatcagact cgggtcattc atatccgcca ggctggcgtc tgcacttttc agcaacgctg
     2461 cagaggtcgt gacgtcctga tcgctccaca gcttccgtcc ggcgagtttc accagaaaat
     2521 cggcctgagc cagcagccag gttttagcat cgctgccgga aatggtggcg atcttttgtt
     2581 ggacttcatc caactgtttt gccagcgttt cttgctgacg attcgcctgc ttaagctgcg
     2641 cagcttgttg cttaataatg ccttccagct cggctttttg gctctcctgg gctttttgca
     2701 atgccgtcag ttggttagcc agggcatcgc tggtggcggt ctgattgacg gcctgttgtt
     2761 taccccagcc atacaaaccg acacccgccg ccagagcaat agcgatagcc accgcgctga
     2821 gaatcaatgc agtattgttc ttactctttt tttctgttgc gacaggttgt gacgtggtgt
     2881 ccacggcctc cctggtctct tcaaccacgg cggaggtttt ttcttgttcc gtcattatgg
     2941 cttcctgtta tgagagttat tgtaatgccc gtaaaagcgc atcgttgtca gcgttatcgg
     3001 cgaccttaat gtcttgccag cccagttccc gggcgagttt cgccaaacgc tcactgacga
     3061 ccaatagtcg acagtgtagt aaccagtgct cacgatacca ttgtgggatc agcgaccaga
     3121 gttgctgcaa catttcaccg ctggtaacaa cgaccatcgt cacctcgcgg gattgccagc
     3181 gcatcgcttc ttctgcacca tcgtaatgga ttgcgcatcg ttgataacat tcacaaaaag
     3241 tgacctcagc accgcgcacc gtcagggtat ccccaattag ctcacgaccg ccattgccac
     3301 gtaatatcag cgcacgtttg cccgcaatat tttgtaattc aggtaattgt agcaagactt
     3361 cgctgatttc ccgatcctgt gggtagagaa tcttctgtcc gcttacggta tgtagcgcca
     3421 gtgcggtggt gcgtccaatg gcgaaataag taggtagttg gggccattta cgatcttgct
     3481 gatgcagctg tgattgggca aaagcaaccg cgtgttgcga gagggcaaac aacagatcgc
     3541 tctcccccag cgctgccagt tgatcagcaa gttgcggtaa ttgtcgaccc ggagaaaact
     3601 caatcagcgg aaaatgccag gccacctgcc ccagtgtgcg cagacggctc actaactctt
     3661 ctccagcggg agacgggcgg gtgaccagga tactcatgcc ggggcctctc cgttatagac
     3721 ttcagcgagg atctcgcgcg cgccgttatt cagtagctct tctgccagcg aaatccccat
     3781 ttgttcggca tcttgcggcg caccgcggcg ttcaccgcga ataatctgcg aaccgtccgg
     3841 cgcgccgacc agcgcacata gccagatttc gccatcaata agctcggcgt agctaccaat
     3901 tggcacctga catccgcctt cgagacgggt attcatggcg cgttctgcgg taacgcgcag
     3961 tgcagtttcg tggtgattca gcgcggcaag cagctcgcga gtgcgtgtat catcaaggcg
     4021 gcattcaata ccgactgcac cttgtcctac cgccggaaga gaaatctcgg gtggtaacgc
     4081 ggcgcgaatg cgggactcca gacctaaacg ttttagtccg gctacggcaa gaatgatggc
     4141 atcgtattcg ccgttatcta gtttgctcag gcgagtgccg acgttgccgc gcagggagcg
     4201 gataatcaga tccgggcggc gttcagccag ttggcactgg cgacgtaaac tggacgtccc
     4261 gacgatactg cctgctggta acgcatccag actgtcatag ttattggaca caaaggcatc
     4321 gcgaggatct tcacgctcgc aaatagtgac cagccccaga ccttgcggga attcaaccgg
     4381 cacatctttc attgaatgta cggcgatatc agcgcgattc tccaggagcg cgacttccag
     4441 ctcttttaca aataagcctt ttccgcctac tttcgccagc ggcgtatcaa gaatcacatc
     4501 gccgcgcgtc accatcggta ccagttcaac gaccaggccc ggatggctcg ccatcaactt
     4561 gtctttgaca tagtgtgcct gccagagtgc aagtgggctt tggcgtgtgg caattcttaa
     4621 aacattgtct aacatgcttg ttaccgtcat tatcatccgt ggtccatcct aacatccttg
     4681 ccagagtgat gtcagtgttg tggtgaaacg tagacgcctg cgcaaaccgt aaaatgaggt
     4741 ctggcagtgg atcctgacag gcgtttcacg ccgtcgtaat aaggaattta cagagaataa
     4801 acggtgctac acttgtatgt agcgcatctt tctttacggt caatcagcaa ggtgttaaat
     4861 tgatcacgtt ttagaccatt ttttcgtcgt gaaactaaaa aaaccaggcg cgaaaagtgg
     4921 taacggttac ctttgacata cgaaatatcc cgaatgccgc gtgttaccgt tgatgttggc
     4981 ggaatcacag tcatgacggg tagcaaatca ggcgatacgt cttgtacctc tatattgaga
     5041 ctctgaaaca gagactggat gccataaatc aattgcgtgt ggatcgcgcg cttgctgcta
     5101 tggggcctgc attccaacag gtctacagtc tactgccgac attgttgcac tatcaccatc
     5161 cgcgaatgcc gggttacctt gatggtaacg ttcccaaagg catttgcctt tacacgcctg
     5221 atgaaactca acgccactac ctgaacgagc ttgaactgta tcgtggaatg tcagtacagg
     5281 atccaccgaa aggtgagctt ccaattactg gtgtatacac catgggcagc acctcgtccg
     5341 tagggcaaag ttgttcctct gacctggata tctgggtctg tcatcaatcc tggctcgata
     5401 gcgaagagcg ccagttacta caacgtaaat gcagcctgct ggaaagctgg gccgcctcgc
     5461 tgggtgtgga agtcagcttc ttcctgattg atgaaaaccg cttccgtcat aatgaaagcg
     5521 gcagcctggg gggcgaagat tgtggctcca cccagcatat actgctgctt gacgaatttt
     5581 atcgtactgc cgtgcgtctc gccggtaagc gtattctgtg gaatatggtg ccgtgcgacg
     5641 aagaagagca ttacgacgac tatgtgatga cgctttacgc gcagggcgtg ctgacgccaa
     5701 atgaatggct ggatctcggt ggcttaagct cgctttctgc tgaagagtac tttggtgcca
     5761 gcctttggca gctctacaag agtatcgatt ccccatacaa agcggtgctg aaaacactgc
     5821 tgctggaagc ctattcctgg gaatacccga acccacgtct gctggcgaaa gatatcaaac
     5881 agcgtttgca cgacggcgag attgtatcgt ttggcctcga tccatactgc atgatgctgg
     5941 agcgtgttac tgaatacctg acggcgattg aagatttcac ccgtctggat ttagtacgtc
     6001 gctgcttcta tttaaaagtg tgcgaaaaac tcagccgtga acgcgcctgt gtaggctggc
     6061 gtcgcgcagt gttgagccag ttagtgagcg agtggggttg ggacgaagct cgtctggcaa
     6121 tgctcgataa ccgtgctaac tggaagattg atcaggtgcg tgaggcgcac aacgagctgc
     6181 tcgacgcgat gatgcagagc taccgtaatc tgatccgctt tgcgcgtcgc aataacctta
     6241 gcgtctccgc cagtccgcag gatatcggcg tactgacgcg taaactgtat gccgcgtttg
     6301 aagcattgcc aggtaaagtg acgctggtaa acccgcagat ttcacccgat ctctcggaac
     6361 cgaatctgac ctttatttat gtgccgccgg gccgggctaa ccgttcaggt tggtatctgt
     6421 ataaccgcgc gccaaatatt gagtcgatca tcagccatca gccgctggaa tataaccgtt
     6481 acctgaataa actggtggcg tgggcatggt ttaacggcct gctgacctcg cgcacccgtt
     6541 tgtatattaa aggtaacggc attgtcgatt tgcctaagtt gcaggagatg gtcgccgacg
     6601 tgtcgcacca tttcccgctg cgcttacctg caccgacacc gaaggcgctc tacagcccgt
     6661 gtgagattcg ccatctggcg attatcgtta acctggaata tgacccgaca gctgcgttcc
     6721 gcaatcaggt ggtgcatttt gatttccgta agctggatgt cttcagcttt ggcgagaatc
     6781 aaaattgcct ggtaggtagc gttgacctgc tgtaccgcaa ctcgtggaac gaagtgcgta
     6841 cgctgcactt caacggcgag caatcgatga tcgaagccct gaaaactatt ctcggcaaaa
     6901 tgcatcagga cgccgcaccg ccagatagcg tggaagtctt ctgttatagc cagcatctgc
     6961 gcggcttaat ccgtactcgc gtgcagcaac tggtttctga gtgtattgaa ctgcgccttt
     7021 ccagcacccg ccaggaaacc gggcgtttca aggcgctgcg cgtttctggt caaacctggg
     7081 gcttgttctt cgaacgcctg aatgtatcgg tacagaagct ggagaacgcc atcgagtttt
     7141 atggcgctat ttcgcataac aaactgcacg gcttgtcagt gcaggttgaa accaatcacg
     7201 tcaaattacc ggcggtggtg gacggctttg ccagcgaagg gatcatccag ttctttttcg
     7261 aagaaacgca agacgagaat ggctttaata tctacattct cgacgaaagc aaccgggttg
     7321 aggtgtatca ccactgcgaa ggcagcaaag aggagctggt acgtgacgtc agtcgcttct
     7381 actcgtcatc gcacgaccgc ttcacctacg gctcaagctt catcaacttc aacctgccgc
     7441 agttctatca gattgtgaag gttgatggtc gcgaacaggt gattccgttc cgaacaaaat
     7501 cgattggcaa cctgccgccc gccaatcagg atcacgatac gccgctatta cagcagtatt
     7561 tttcgtgatg agcgtgccgg aaagcgaggc ttatccggca tgcaatctta gcggaaactg
     7621 actgtttcac ccgcctgttg cgtcgccgcc tgttccagca aatcccagaa ggtttcgccg
     7681 ctgcgatcgc aaatccattc atcgcctttc aggtcaaaat ggtagccgcc ctgtttggtt
     7741 gccagccaca cctggtgcag cggctcctgg cggttaataa tgattttgct gccgttttca
     7801 aaggtaatgg tcagtacgcc gccgttgatt tcgcagtcga tatcgctgtc gccatccctg
     7861 tcgtccagac gctcttcaat ggtcagccac agttgatcag ccaggcgatg aaattcactg
     7921 tcgttcattg ttgtatcctg tttttaagtg atggcggcag tatagcggca tggggtcagg
     7981 gcttcaaagt ttgcacctct gctgctgcgt tccggcatga ttcatccgtc accggaataa
     8041 tgatgtctct gttttgaact tcacgtacgt tctcctctct gacacgagtt ttcgccgggc
     8101 agggggaggg cgtaatcctt acctctgtat cggtccatgt ataaacattg cgatcgtcat
     8161 aagcgaagcg atcgttttta gttagcgaac cgttaactgg aataatttgc aagctctttg
     8221 accgtaattg gcggataaag taagcatgct gtttgccgct attaaatgag cgatcaatga
     8281 ccaggtctgg cgtatccagc gggattatct caagctggtc ttcaccaaat gtcgataatt
     8341 ttgttacata cagccgatct ttatattgcg caatattgcc agtaatcaga tggaatt
//
LOCUS       DAZSNO010000165         8350 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZSNO010000165 DAZSNO010000000
VERSION     DAZSNO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.167"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            268..1200
                     /gene="glaH"
                     /locus_tag="ACHWN2_003473"
     CDS             268..1200
                     /gene="glaH"
                     /locus_tag="ACHWN2_003473"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR8932868.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1220..2488
                     /gene="lhgO"
                     /locus_tag="ACHWN2_003474"
     CDS             1220..2488
                     /gene="lhgO"
                     /locus_tag="ACHWN2_003474"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR8932869.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2511..3959
                     /gene="gabD"
                     /locus_tag="ACHWN2_003475"
                     /pseudo
     CDS             2511..3959
                     /gene="gabD"
                     /locus_tag="ACHWN2_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            3967..5253
                     /gene="gabT"
                     /locus_tag="ACHWN2_003476"
                     /pseudo
     CDS             3967..5253
                     /gene="gabT"
                     /locus_tag="ACHWN2_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            5491..6891
                     /gene="gabP"
                     /locus_tag="ACHWN2_003477"
     CDS             5491..6891
                     /gene="gabP"
                     /locus_tag="ACHWN2_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR8932870.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6912..7574
                     /gene="csiR"
                     /locus_tag="ACHWN2_003478"
     CDS             6912..7574
                     /gene="csiR"
                     /locus_tag="ACHWN2_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR8932871.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7575..8024)
                     /gene="kbp"
                     /locus_tag="ACHWN2_003479"
     CDS             complement(7575..8024)
                     /gene="kbp"
                     /locus_tag="ACHWN2_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR8932872.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8108..8266)
                     /locus_tag="ACHWN2_003480"
     CDS             complement(8108..8266)
                     /locus_tag="ACHWN2_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR8932873.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1888 a         2196 c         2321 g         1945 t
ORIGIN      
        1 agtgaagcca tttactgttt tttatcgacc agataatctg ttctctaatg ttaactcccc
       61 ctaacctgtt gctttagtta ttcatttcct gtctcacttt gccttaatac cctacgttaa
      121 atgttactaa tttgttgctt ttgatcacaa taagaaagca atatgtcgct tttgtgcgca
      181 tttttcagaa atgtagatat ttttagatta tggctacgaa atgagcatcg ccatgtcacc
      241 ctacatctca taagaggatc gcttctgatg aatgcactga ccgccgtaca aaataacgct
      301 gtcgattcag gccaggacta tagcggattc actctcatcc cgtcggcgca atccccgcgt
      361 ctgctggaac tcaccttcac cgaacagacg accaaacagt ttctcgaaca ggttgccgaa
      421 tggcccgtgc aggcgctgga gtacaaatcg tttctgcgtt ttcgggtagg caaaattctt
      481 gacgatctgt gtgcgaatca gctgcaacca ttgctgttga agaccctgct aaaccgtgct
      541 gaaggtgcgc tgttgatcaa tgcggtaggt atcgatgatg tggcgcaggc agatgagatg
      601 gtgaagctgg caacggcggt agcgcatctg attggtcgct caaatttcga tgcgatgagc
      661 ggtcagtatt acgcgcgttt cgtggtgaaa aatgtcgata actcagacag ctatctgcgt
      721 cagccgcacc gcgtgatgga actgcacaac gacggcacgt atgtcgaaga gatcaccgat
      781 tacgtgctga tgatgaaaat cgatgactgg gaacatctgg accactattt ccgccatcca
      841 ctggcgcgtc gcccgatgcg ctttgccgcg ccgccgagca aaaacgtcag caaagatgtt
      901 ttccatccag tgttcgacgt tgatcaacag ggtcgcccgg tgatgcgcta tatcgaccag
      961 ttcgtccagc caaaagattt tgaagaaggc gtgtggttga gcgagctttc cgatgccatt
     1021 gaaaccagca aaggcattct ttctgtaccc gttcccgttg gcaaattcct gttgataaac
     1081 aacctgttct ggctgcacgg tcgcgaccgc tttactccgc acccggatct gcgccgtgaa
     1141 ctgatgcgtc agcgtggcta tttcgcttac gccactcacc actaccagac gcatcagtaa
     1201 gcgcaaagga attgagcgga tgtatgattt tgtgattatt ggcggcggca tcatcggcat
     1261 gtcgaccgcc atgcaactga ttgatgttta tccggatgcc cgcattgcgt tgctggaaaa
     1321 agagtccggc ccggcctgtc accagacggg ccacaacagc ggcgtgatcc atgccggggt
     1381 ctattacacg cccggcagcc tgaaggcgca gttttgcctg gcggggaacc gtgccaccaa
     1441 agccttttgc gatcaaaacg gcattcgcta tgacaactgc ggcaagatgc tggtcgccac
     1501 gtctcagctc gaaatggaac ggatgcgcgc cttatgggaa cgcacagcgg cgaacggtat
     1561 cgagcgcgag tggataaacg ccgtggaact gcgcgagcgc gaaccgaata tcaccgggct
     1621 tggtggcatt tttgtgccgt ccagcggcat tgtcagctac cgcgaagtga cggcggcaat
     1681 ggcaaaaatc ttccaggcca gaggcggcga gattatttat aacgccgaag tcagcgccct
     1741 cagtgagcat aaaaacggcg tggtgatacg cacccgtcag ggcggtgaat atgaagcatc
     1801 gacgctgatt agctgttccg ggctgatggc tgaccggctg gtgaaaatgc tcggcctcga
     1861 accgggcttt attatctgcc cgttccgtgg cgagtatttc cgccttgcgc cggagcataa
     1921 ccagattgtt aaccacctga tttaccccat tcccgacccc gcgatgccat ttttgggcgt
     1981 gcatctcacc cgcatgatcg acggcagcgt gaccgtcggg ccaaacgcgg tgctggcttt
     2041 caaacgcgaa ggctatcgca agcgcgactt ctcatttagt gacacgctgg agattttgag
     2101 ctcgtcgggg attcgccggg tgctgcaaaa ccatctacgc tcaggactgg gcgagatgaa
     2161 aaactcgctg tgcaaaagcg gctatctgcg gctggtgcaa aagtattgtc cccggctttc
     2221 gttaagcgat ctccagccct ggcccgccgg tgtgcgggcg caggcagtat cgccggacgg
     2281 caagctgatt gacgattttc tgtttgtcac caccccacgc acgatccaca cctgcaatgc
     2341 gccctccccg gcagcgacat cagcaattcc tattggtgcg catattgtca gcaaggtaca
     2401 aacgctgttg gcaagccaga gtaaccccgg acgcacgctg cgagcggcac gtagtgtgga
     2461 tgccttacac gccgcattta atcaataacc tttgaaaaca ggacgtaaca atgaaactta
     2521 acgacagtaa cttattccgc cagcaggcgt tgattaacgg ggaatggctg gacgccaaca
     2581 atggtgaagt catcgacgtc accaatccgg cgaacggcga caagctgggt agcgtgccga
     2641 aaatgggcgc agatgaaacc cgcgccgcta tcgacgccgc caaccgcgcc ctgcccgcct
     2701 ggcgtgcgct caccgccaaa gaacgcgcca ccattctgcg caactggttc aatttgatga
     2761 tgcagcatca ggacgattta gcgcgcctga tgaccctcga acagggtaaa ccgctggctg
     2821 aagcgaaagg cgaaatcagc tacgccgcct ccttcattga gtggtttgct gaagaaggca
     2881 aacgtattta tggcgacacc attcctggtc atcaggccga taagcgcctg attgttatca
     2941 agcagccgat tggcgtcacc gccgccatca cgccgtggaa ctttccggcg gcgatgatta
     3001 cccgtaaagc cggtccggcg ctggcggcag gctgcaccat ggtgctgaag cccgccagtc
     3061 agacgccgtt ctctgcgctg gcgctggcgg aactggcgat tcgcgcgggc attccggctg
     3121 gcgtgtttaa cgtggtcacc ggttcggcag gcgcggtcgg taatgaactg accagcaacc
     3181 cgctggtacg caaactgtcg tttaccggtt agaccgagat tggccgccag ttaatggaac
     3241 agtgcgcgaa agacatcaaa aaagtgtcgc tggagctggg cggcaacgcg ccgtttatcg
     3301 tctttgacga tgccgatctc gataaagccg tggaaggcgc gctggcctcg aaattccgca
     3361 acgccgggca aacctgcgtc tgcgccaacc gtttatacgt gcaggacggc gtgtatgacc
     3421 gctttgccga aaaattacag caggcgggga gcaaactgca catcggcgac gggctggaga
     3481 aaggcgtcac catcgggccg ctgatcgatg aaaaagcggt agcaaaagtg gaagagcata
     3541 ttgccgatgc gctggagaaa ggtgcgcgtg tggtttgcgg cggtaaagcg cacgaacgcg
     3601 gcggcaactt cttccagcca accattctgg tggacgttcc ggccaacgcc aaagtgtcga
     3661 aagaagagac gttcggcccc ctcgccccac tgttccgttt taaagatgaa gccgatgtga
     3721 ttgcacaagc caatgacacc gagtttggtc ttgccgccta tttctacgcc cgtgatttaa
     3781 gccgcgtctt ccgcgtcggc gaggcgctgg agtacggcat cgtcggcatc aataccggca
     3841 ttatttccaa tgaagtggcc ccattcggcg gcatcaaagc ctcgggtctg ggtcgtgaag
     3901 gttcgaagta tggcatcgaa gattacttag aaatcaaata tatgtgcatc ggtctttaac
     3961 tggagaatgc gaaggaacag caataaagag ttaatgcagc gccgcagtca ggcgattccc
     4021 cgtggcgttg ggcaaattca cccgattttc gctgaccgcg cggaaaactg ccgggtgtgg
     4081 gacgtttaag gccgtgagta tcttgatttc gcgggcggga ttgcggtgct caataccggg
     4141 cacctgcatc cgaaggtggt ggccgcggtg gaagcgcagt tgaaaaaact gtcgcacacc
     4201 tgcttccagg tgctggctta cgagccgtat ctggagctgt gcgagattat gaatcagaag
     4261 gtgccgggcg atttcgccaa gaaaacgctg ctggttacga ccggttccga agcggtggaa
     4321 aacgcggtaa aaatcgcccg cgccgccacc aaacgtagcg gcaccatcgc ttttagcggc
     4381 gagtatcacg ggcgcacgca ttacacgctg gcgctgaccg gcaaggtgaa tccgtactct
     4441 gcgggcatgg ggctgatgcc gggtcatgtt tatcgcgcgc tttatccttg cccgctgcac
     4501 ggcataagcg aggatgacgc tatcgccagc atccaccgga tcttcaaaaa tgatgccgcg
     4561 ccggaagata tcgccgccat catgattgag ccggttcagg gcgaaggcgg tttctacgcc
     4621 gcgtcgccag cctttatgca gcgtttacgc gctctgtgtg acgagcacgg gatcatgctg
     4681 attgccgatg aagtgcagag cggcgcgggg cgtaccggca cgctgtttgc gatggagcag
     4741 atgggcgtgg cgccggatct taccaccttt gcgaaatcga tcgcaggcgg tttcccgctg
     4801 gcgggcgtca ccgggcgcgc ggaagtgatg gacgctgttg caccgggcgg tctgggcggc
     4861 acctatgccg gtaacccgat tgcctgcgtg gctgcgctgg aagtgttgaa ggtgttcgag
     4921 caggaaaatc tgctgcaaaa agccaacgat ctggggcaga agctaaaaga cggattgttg
     4981 gcgatagccg aaaaacaccc ggagatcggc gacgtacgcg ggctgggatc gatgattgcc
     5041 atcgagctgt ttgaagatgg cgatcacagc aaaccggacg caaaactcac cgccgagatc
     5101 gtggcacgcg cccgcgataa gggcctgatt cttctctcct gcggcccgta ttacaacgtg
     5161 ctgcgcatcc ttgtaccact caccattgaa gacgctcaga tccgtcaggg tctggagatt
     5221 atcggtcagt gttttgatga ggcgaagcag tagcaccgtc cccatgcagg aggcgacgct
     5281 gcgcgtcttg tccggcctac gagaatccaa gtcggataag gcgtttacgc cgcatccgac
     5341 aatctgtacg tgaacaggaa taaatctatg tcggccggat aaggcggagc cgctctccgg
     5401 caaaaagaat caataacaat taaacgcgtg acccgacgcg ggaaatgtcg gggcgctctc
     5461 ccaagtgaca cactttcgag aggattcagg atggggcaat catcgcaacc acacgagtta
     5521 ggcggcgggc tgaaatcacg ccacgtcacc atgttgtcta ttgccggtgt tatcggcgca
     5581 agtctgtttg tcggttccag cgtcgccatc gccgaagcgg acccggcggt attactggcc
     5641 tatctgttcg ccggattact ggtggttatg attatgcgga tgctggcgga aatggcagtt
     5701 gccacgcccg ataccggttc gttttccacc tatgccgata aagccattgg ccgctgggcg
     5761 ggctatacca tcggctggtt gtactggtgg ttttgggtac tggttatccc actggaagcc
     5821 aacatcgccg ccatgatcct gcactcgtgg gttccaggca ttcccatctg gttattttcc
     5881 ctcgtcatta ccctcgcctt aactggcagt aatttattaa gcgttaaaaa ctacggcgaa
     5941 tttgagttct ggctggcgct gtgcaaagtc atcgctatcc tggcctttat tttccttggt
     6001 gcagtcgcaa ttagcggttt ttacccttat gccgaagtga gcgggatctc aagattgtgg
     6061 gatagcggcg gctttatgcc caacggtttc ggtgcggtat taagcgcgat gttgatcacc
     6121 atgttctcgt ttatgggcgc agaaattgtc accattgccg ccgcggaatc cgacatgccg
     6181 gaaaaacata ttgtccgcgc caccaactcg gttatctggc gtatttctat cttctatttg
     6241 tgctctattt ttgtcgtcgt ggcattaata ccgtggaata tgccggggct gaaagccgtt
     6301 ggatcttatc gctcggttct ggaattgctc aatattcccc atgcgaaatt aatcatggac
     6361 tgtgtgatat tactttccgt aaccagttgc ctgaactcgg ccctgtatac cgcctcaagg
     6421 atgctctact ccttaagccg tcgcggtgat gctcccgcgg taatgggtaa aatcaaccgc
     6481 agtaaaaccc cgtacgtggc ggtgttactc tccaccggcg cagcattctt aacggtggtg
     6541 gtgaactatt acgcgcctgc gaaggtattt aaatttctga tcgacagctc cggcgctatc
     6601 gccctgctgg tttatttagt catcgccgtt tcacagttgc ggatgcgcaa aattctgcga
     6661 gcagaaggaa gcgaaattcg cttgcgaatg tggcttgatc cctggctcac ctggctggtc
     6721 atcggcttta ttacctttgt gttggtagtg atgctattcc gtccggcgca acagttagaa
     6781 gtgatctcca ccggcttatt agcgataggg attatctgta ccgtgccaat tatggctcgc
     6841 tggaaaaagc tggtattgtg gcaaaaaaca cccgttcata atacgcgctg ataatgatca
     6901 ggagtcacac catgaccatt acgtctctgg atggctatcg ctggctgaag aacgatatta
     6961 ttcgcggtaa ttttcaaccg gatgaaaaat tacggatgag tttactgaca tcgcgttatg
     7021 cacttggcgt tgggccgttg caggaagcac tttctcaact ggtggcggaa cggctggtca
     7081 cggtggtgaa tcaaaaaggg tatcgggtgg cgtctatgtc agagcaggag ctgctcgata
     7141 ttttcgacgc ccgcgccaat atggaagcga tgctggtcag tctggcgatt gcccgcggtg
     7201 gcgatgaatg ggaggcagac gttctcgcaa aagcgcatct gctcagtaag ctggaagcct
     7261 gtgacgccag cgagaaaatg cttgatgagt gggatctgcg tcatcaggcg tttcatacag
     7321 caattgtggc gggctgtggt tctcactatt tgctgcaaat gcgtgaacgg ttgtttgatc
     7381 tggcggcgcg ttatcgattt atctggctgc ggcgaacggt gctttcggtg gaaatgctgg
     7441 aggataaaca cgatcagcac cagaccctga ctgcggcggt actgccgcga gatacagcgc
     7501 gcgccagtga gttaatgcgc cagcatttac tgacgccaat tcccattatc cagcaggcga
     7561 tggctggcaa ttaactactc ttccggaata cgcaacactt gccccggata aattttatcc
     7621 gggcttttta gcatcggttt attcgcttcg aagattttat tgtacagatt agcgttaccg
     7681 tagacctgtt tggaaatggc actcagagtg tcgccagact taacggtata aaactggctg
     7741 gcagtggctg gtgtcgccgt tttcacctga tcatcaacac tggcaatacc ggaaatattc
     7801 cccaccgcaa caaggatttt ctccttcgcc tcctgactca ggccgtcacc agtgaccgtc
     7861 gctttgccgt cggcaatttg aatgttcact ttatcggcat caggtatacc ggttttgctc
     7921 agatgctcct gcaccttctt cgcctgatcg tctttatcgt gctgacctgt aaccgcatcc
     7981 cagagttttt ctccggcatc tttaacaaaa ttgaacagac ccatagctac ctcattcgtt
     8041 aacggaaaca ctcagaaagt gtagcagagg aacgcggcgc gttaacgccg cgggggtgag
     8101 cacaggttta atcgcgggtt tgcacccaga acgcgtgaat caaaccgggg atatacccaa
     8161 gcagtgtcag cagaatatta ataatgaacg cccaaccgaa ccctttaccg agcagcacgc
     8221 cgatcggcgg cagaatgatg gtgatgacga ttctccagaa acccatatgt actccctata
     8281 agaaaattac tcattgttta aaaagagatt ttatctctta agcgtagtaa ttttagcgga
     8341 gcctgccagt
//
LOCUS       DAZSNO010000166         8274 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZSNO010000166 DAZSNO010000000
VERSION     DAZSNO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.168"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..215
                     /locus_tag="ACHWN2_003481"
     CDS             <1..215
                     /locus_tag="ACHWN2_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932874.1"
                     /translation="TLNSNQFENKDATKDKLDNRGSIKGKLNNIYGKSIDYAALRHRD
                     IIIAKIDLFIQRITHNLWHARKKMCF"
     gene            239..946
                     /locus_tag="ACHWN2_003482"
     CDS             239..946
                     /locus_tag="ACHWN2_003482"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR8932875.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            1309..2346
                     /gene="dusA"
                     /locus_tag="ACHWN2_003483"
     CDS             1309..2346
                     /gene="dusA"
                     /locus_tag="ACHWN2_003483"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR8932876.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2480..2722
                     /gene="pspG"
                     /locus_tag="ACHWN2_003484"
     CDS             2480..2722
                     /gene="pspG"
                     /locus_tag="ACHWN2_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR8932877.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(2888..3871)
                     /locus_tag="ACHWN2_003485"
     CDS             complement(2888..3871)
                     /locus_tag="ACHWN2_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR8932878.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3954..5369
                     /gene="dnaB"
                     /locus_tag="ACHWN2_003486"
     CDS             3954..5369
                     /gene="dnaB"
                     /locus_tag="ACHWN2_003486"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR8932879.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5422..6501
                     /gene="alr"
                     /locus_tag="ACHWN2_003487"
     CDS             5422..6501
                     /gene="alr"
                     /locus_tag="ACHWN2_003487"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR8932880.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6754..7947
                     /gene="tyrB"
                     /locus_tag="ACHWN2_003488"
     CDS             6754..7947
                     /gene="tyrB"
                     /locus_tag="ACHWN2_003488"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR8932881.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         2065 a         1916 c         2112 g         2181 t
ORIGIN      
        1 aaacactcaa ttcaaatcaa ttcgaaaata aagatgcaac caaagataaa ttggataata
       61 gaggttcaat caaaggtaaa ttaaataata tatatggtaa atctattgat tatgcagcgc
      121 tgcgtcatcg ggatataatt attgcaaaaa tagatttgtt tattcaacga atcacgcata
      181 atttatggca tgcacgtaaa aaaatgtgtt tttaattgag cacataaatg atctcaaaat
      241 gtgggtcaat aagtatactg acgattgcac tgatgaggat ttaaacgatc gtgactttat
      301 tgcatcagtt gttgaccggg ctatttttca tttcgcgatt aatagtatat gtaatcctgg
      361 ggataataaa gatgcaatgc ccattgaaca atgtactttt gatgtagaaa ctaagaatgg
      421 ccttccctcc acggttcagc tattttatga ggaatctaag gataatgaac ctttagcgaa
      481 tatacatttt caagcaatag gttctggttt tttaacgttt gttaatgcct gccaggaaca
      541 tgatgacaac agcttaaaat tatttgcttc gctgttaatt tcactttcat attctagtgc
      601 ctacgcagat ttatcagaaa cagtgtatat taatgaaaat aatgagagct acctgaaagc
      661 tcagtttgaa aaattatatc aacgtgatat gaagaagtac ctgggagaga tgaagcgtct
      721 ggcagatggg ggagaaatga attttgatgg ctatctggat aagatgtcac atctggtgaa
      781 tgaaggaacg ctcgatcctg atattttaag caaaatgcga gatgctgcac cacaattaat
      841 tagcttcgcg aagtcgtttg acccaacctc aaaggaagag attaaaatac ttacagacac
      901 ttctaaatta atttatgact tgttcggggt taaatcggag aaataatatg tgaagttctt
      961 cgatagtatg gaaggcatta tataaaagga cccaatattt attgggttct tttttctcta
     1021 tcaatgctat tagcagggag atatatcacc agagtttaat gtgtgatttt ttatttatcg
     1081 tcgaacctgg attgtttatc attggcctta acaaagttaa cggctaataa gattatttcc
     1141 atcacttcgt gagagcttca tgccttgaga ggatctcaat tttcttttgc aatgagacag
     1201 gcgcttcctg ttgttatggt atagtacccc gctattgagc ctcctgaaca gtgaagctga
     1261 ataacataac cccatgatat atcgataaaa taatctctac atttgaaaat gcacggtaat
     1321 tctgaaatgc aaaaaatcaa ccaaaccagc gcaatgcctg aaaaaactga cgttcactgg
     1381 agtggtcggt ttagtgttgc accaatgctc gactggacgg acagacattg ccgctatttc
     1441 ttgcgtctgc tttcccgcaa tacgttgctt tataccgaaa tggtgaccac aggggcgata
     1501 attcacggta aaggtgatta tctggcgtac agtgaagaag aacatccggt agcgttgcaa
     1561 ctgggcggta gcgatccggc ggcgctggcg cagtgtgcaa agctggcaga agcgcgcgga
     1621 tatgatgaga tcaacctgaa tgtcggctgc ccgtctgacc gggtgcagaa cggcatgttt
     1681 ggtgcgtgtc tgatgggtaa tgcgcagctg gttgccgact gcgtgaaagc gatgcgcgat
     1741 gtggtgtcga ttccggtgac ggtgaaaacg cgtattggca tcgacgacca ggacagctat
     1801 gaatttctct gcgatttcat caataccgtt tccggcaaag gcgagtgtga gatgttcatc
     1861 atccacgcac gtaaagcctg gctttcgggg ttaagcccga aagaaaaccg tgaaatcccg
     1921 ccgctcgatt atccgcgtgt gtatcaactg aagcgtgact tcccgcatct gacaatgtcg
     1981 attaacggtg gtatcaagtc gctggaagaa gctaaagcgc atttgcaaca tatggatggc
     2041 gtgatggtcg ggcgcgaggc gtatcagaat ccgggtattc tggcggcggt agaccgggag
     2101 atctttggtt cctcggatac cgatgccgat ccggtggcag tagtgcgtgc catgtatccg
     2161 tacattgagc atgaactcag ccaggggacg tatctcggcc atattatccg gcatatgctg
     2221 ggtttgttcc agggtattcc tggcgcgcgg cagtggcggc gttatttaag tgaaaatgcc
     2281 cataaagcgg gtgctgacat taacgtgctg gaacacgcgc tcaaactggt ggcggataag
     2341 cgttaacttt tcaccaaaaa gtagtcaaat tcaccacgcc ctgcgcaccg tcgcggggcg
     2401 ttttgctgtt aaatcaatag attatttttg gcatgattct tgtaatgcca gcaagagatt
     2461 tcatatttgg gagagcatca tgctggaact actttttgtg attggctttt ttgtcatgct
     2521 gatggtcacc ggcgtttcgt tgctgggcat tatcgccgcg ctggttgtgg cgacggccat
     2581 tatgttcctc ggcggtatgc tggcattgat gattaagttg ctgccgtggt tactactggc
     2641 gattgcggtg gtgtgggtta ttaaggcgat taaagcacca aaaatgccga aatatcagcg
     2701 ttatgaccgc tggcgttact aagggattgt gcggatgatc acaacctaag gttttatcct
     2761 tagaacaaaa taggaattga taatcaaatc tgtcactatt gcgcctctaa cagattcatc
     2821 gtgctgtacc ctacatacag ccgaactata aaaagaaagg gcttcccagg tggaagccct
     2881 atttctttta tggaatcagc aggctggaac cttgcgtcgc ccggctttcc agaatctcat
     2941 gcgcacgctg cgcatccttc agcggatatt tctgctgctc ggcgacatcg accttaatca
     3001 caccgctggc aatcaaagag aacagttcat tactggcctc ggttaattcc tcccgcgtgg
     3061 tgatatagcc ttgcagggaa gggcgtgtca catacaacga gcctttttga ttgagaatgc
     3121 ctaagttcac accggtaacc gcacctgatg agttgccaaa actgaccatt aagccgcggc
     3181 gttgcaggca atccagcgac cgttcccagg tgtctctgcc cacggaatcg tacactacgc
     3241 gcactttctt accgccggtg atctctttca atcgctcgac cagattctct tcacgatagt
     3301 taataacctg ccacgcgccg gcttttagcg cgctctgcgc tttttgcgcg gttcctacgg
     3361 tgccgatgag tttcgcgccc agggcttttg cccactggca ggcaattaag ccaatgccgc
     3421 cagccgctgc gtggaacagg aactgttcat cgggtttaat ttcataggtt ttgcgcagca
     3481 gataataaac cgttaagcct ttcaggaagg atgccgcagc ttgctcaaaa gaaattgccg
     3541 caggcagaat cgccgcttta tccgcattaa tgttatgcac agagctgtaa gcgcctaacg
     3601 ccgactgcgc atagactaca tgatcgccag ccttaatatg ctttacacca ctgccgactt
     3661 tactcacgat gcctgccgct tcggtaccta atccgctggg tagcgatggc ggcgggtaaa
     3721 ggccgctgcg gatatacgtg tcgataaaat tgatgccgat ggctttattt tcgacctgga
     3781 tttcattctc cgccggatcg gcaggagtga actctacggc ttgaagtact tccgggccac
     3841 cgtgcttgtg aaattcaatt cgtgttgcca tgtgtcctcc agaacgtatc gtcagggtct
     3901 gcttcatatg gtaaagtttc gacccattct ttatttcggt aactccattc actatggcag
     3961 gaaataaacc cttcaacaaa cagcaggctg aaccccgcga acgcgatcca caagttgccg
     4021 ggctgaaagt gcctccgcac tcgatcgaag cggagcagtc ggtgttgggc ggtttaatgc
     4081 tggataacga acgctgggat gatgtagccg agcgtgtggt agcagacgat ttttacaccc
     4141 gcccacaccg tcatatcttt actgaaatgg cgcgtttgca ggaaagcggt agtcctatcg
     4201 atctgattac ccttgcggaa tcgttggaac gccaggggca actcgatagc gtcggtggtt
     4261 ttgcttatct ggcagagctg tcaaaaaata cgccaagtgc ggcgaacatc agtgcttatg
     4321 cggacatcgt gcgtgaacgt gccgttgtcc gtgagatgat ctcggttgcg aatgagattg
     4381 ctgaagccgg ttttgatccg caggggcgta ccagcgaaga tctgctggac cttgctgaat
     4441 cccgcgtctt taaaattgcc gaaagtcgtg caaacaaaga cgaagggccg aagaacatcg
     4501 ccgatgtgct cgacgcaacc gtggcgcgta ttgagcagtt gtttcagcag ccacacgatg
     4561 gcgttaccgg ggtaaacacc ggttatgacg atctcaacaa aaaaaccgct ggcttgcagc
     4621 cgtcggattt gatcatcgtc gccgcgcgtc cgtcgatggg taaaacaaca tttgcgatga
     4681 acctcgtcga aaacgcggca atgttgcagg ataaaccagt acttatcttc tcgctggaga
     4741 tgccatcaga acagatcatg atgcgttctc tggcgtcgct gtcgcgcgtt gaccagacta
     4801 aaatccgtac cgggcagctc gatgatgaag actgggcgcg catttccggc accatgggta
     4861 ttttgctcga aaaacgcaat atctatatcg atgactcttc cggcctgacg ccaacggaag
     4921 tgcgttcccg cgcacgccgt attgcccgtg aacacggcgg catcgggctt atcatgatcg
     4981 actacctgca actgatgcgc gtaccggcgc tttccgataa ccgtacgctg gaaattgcag
     5041 aaatctcccg ctcgctgaaa gcactggcga aagaactgaa cgtgccggtg gtggcgctgt
     5101 cccagttgaa ccgttctctg gaacaacgtg ccgacaaacg tccggtcaac tccgacctgc
     5161 gtgaatctgg ctctatcgag caggatgcgg acttgatcat gtttatctat cgtgatgagg
     5221 tgtatcacga aaacagtgat ttaaaaggca tcgcggaaat tattatcggt aaacaacgta
     5281 acggcccaat cgggacggta cgcctgacct ttaacggtca atggtcgcgc ttcgacaact
     5341 atgcggggcc gcagtacgac gacgaataat aattatttta tgaattaggt aattaaagca
     5401 aacaattatc aaggaacaca aatgcaagcg gcaactgttg tgattaaccg ccgcgctctg
     5461 cgacacaacc tgcaacgtct tcgtgaactg gcacctgcca gtaaaatggt tgcggtggtg
     5521 aaagcgaacg cttatggtca cggtcttctt gagaccgcgc gaacgctccc cgatgctgac
     5581 gcctttggcg tagcccgtct cgaagaagct ctgcgactgc gtgcgggggg aatcaccaaa
     5641 cctgtactgt tactcgaagg cttttttgat gccagagatc tgccgacgat ttctgcgcaa
     5701 cattttcata ccgccgtgca taacgaagaa cagctggctg cgctggaaga ggctagcttg
     5761 gacgagccgg ttaccgtctg gatgaaactc gataccggta tgcaccgtct gggcgtaagg
     5821 ccggaacagg ctgaggcgtt ttatcatcgc ctgacccagt gcaaaaacgt tcgtcagccg
     5881 gtgaatatcg tcagccattt tgcgcgcgcg gatgaaccaa aatgtggcgc aaccgagaaa
     5941 caactcgcta tctttaatac cttttgcgaa ggcaaacctg gtcaacgttc cattgccgcg
     6001 tcgggtggca ttctgctgtg gccacagtcg cattttgact gggtgcgccc gggcatcatt
     6061 ctttatggcg tctcgccgct ggaagatcgc tccaccggtg ccgattttgg ctgtcagcca
     6121 gtgatgtcac taacctccag cctgattgcc gtgcgtgagc ataaagccgg agagcctgtt
     6181 ggttatggtg gaacctgggt aagcgaacgt gatacccgtc ttggcgtagt cgcgatgggc
     6241 tatggcgatg gttatccgcg cgccgcgccg tccggtacgc cagtgctggt gaacggtcgc
     6301 gaagtaccga ttgtcgggcg cgtggcgatg gatatgatct gcgtagactt aggtccacag
     6361 gcgcaggaca aagccgggga tccggtcatt ttatggggcg aaggtttgcc cgtagaacgt
     6421 atcgctgaaa tgacgaaagt aagcgcttac gaacttatta cgcgcctgac ttcaagggtc
     6481 gcgatgaaat acgtggatta atcgttctgt aatatttgat tgtctgtgcc ggatgcggcg
     6541 tgaatgcctt atccggccaa taaaatccta aaaattcaat aagttgatgt tctttcatgc
     6601 tcttataaag gtcgtgcctc tggcggatgt acgtttatca ttagtctcac tctgttgcta
     6661 attgccgttc gctcctgaac atccactcga tcttcgcctt cttccggttt attgtgtttt
     6721 aaccacctgc ccgtaaacct ggagaaccat cgcgtgtttc aaaaagttga cgcctacgct
     6781 ggcgacccga ttcttacgct tatggagcgt tttaaagaag accctcgcag cgacaaagtg
     6841 aatttaagta tcggtctgta ctacaacgaa gacggaatta ttccacaatt gaaagccgtg
     6901 gcggaggcgg aagcgcgcct gaatgcgcag ccccatggcg cttcgcttta tttaccgatg
     6961 gaagggctta acagctatcg ccatgccatt gcgccgctgc tgtttggtgc ggaccatccg
     7021 gtactgcaac aacagcgcgt agcaaccatt caaacccttg gcggctcagg ggcattgaaa
     7081 gtgggcgcgg atttcctgaa acgctacttc ccggaatcag gcgtctgggt cagcgatcct
     7141 acttgggaaa accacgtagc aatattcgcc ggggctggat tcgaagtgag tacttacccc
     7201 tggtatgacg aagtgactaa cagcgtgcgc tttaatgacc tgttggcgac gctgaaaaca
     7261 ttacctgccc gcagtattgt gttgctgcat ccatgttgcc acaacccaac gggtgccgat
     7321 ctcactaatg atcagtggga tgcggtgatt gaaattctca aagcccgcga gcttatccca
     7381 ttcctcgata ttgcctatca aggatttagt gccggtatgg aagaggatgc ctacgctatt
     7441 cgcgccattg ccagtgctgg attacccgct ctggtgagca attcgttctc gaaaattttc
     7501 tccctttacg gcgagcgcgt cggcggactt tctgttctgt gtgaagatgc cgaagccgct
     7561 ggccgcgtac tggggcaatt gaaagcaaca gttcgccgca actactccag cccgccgaat
     7621 tttggtgcgc aggtggtggc tgcagtgctg aatgacgagg cattgaaagc cagctggctg
     7681 gcggaagtag aagagatgcg tactcgcatt ctggcaatgc gtcaggaatt ggtgaaggta
     7741 ttaagcacag agatgccaga acgcaatttc gattatctgc ttaatcagcg cggcatgttc
     7801 agttataccg gtttaagtgc cgctcaggtt gaccgactac gtgaagaatt tggtgtctat
     7861 ctcatcgcca gcggtcgcat gtgtgtcgcc gggttaaata cggcaaatgt acaacgtgtg
     7921 gcaaaggcgt ttgctgcggt gatgtaatgc aggaaagcag gctggagtta cccagcctgc
     7981 agtgaaatta aactgtcgtc gctttcacta tttctttgta gatgattttt ttgatgccgt
     8041 tgttctacgt gagagataat aaaacgttgt cagttctttt attgttaagt ttatcccaat
     8101 tatctggaat tcctttccct attttttcgt gtggagtaat aatttctgca catctaacca
     8161 ttattaaatt taataataag tatcgggttt gttacgatag aatatttcta tatcctcaag
     8221 aatatgcttt gccttattgc ctgtgacttt ctgagattca attatagtat cgtt
//
LOCUS       DAZSNO010000167         8225 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZSNO010000167 DAZSNO010000000
VERSION     DAZSNO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.169"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..306
                     /locus_tag="ACHWN2_003489"
     CDS             <1..306
                     /locus_tag="ACHWN2_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR8932882.1"
                     /translation="LLIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMAL
                     SSTLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            278..2205
                     /locus_tag="ACHWN2_003490"
                     /pseudo
     CDS             278..2205
                     /locus_tag="ACHWN2_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2189..2392
                     /locus_tag="ACHWN2_003491"
     CDS             2189..2392
                     /locus_tag="ACHWN2_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFR8932883.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2389..3982
                     /locus_tag="ACHWN2_003492"
                     /pseudo
     CDS             2389..3982
                     /locus_tag="ACHWN2_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3972..5477
                     /locus_tag="ACHWN2_003493"
     CDS             3972..5477
                     /locus_tag="ACHWN2_003493"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR8932884.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5514..5861
                     /locus_tag="ACHWN2_003494"
     CDS             5514..5861
                     /locus_tag="ACHWN2_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR8932885.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5919..6947
                     /locus_tag="ACHWN2_003495"
     CDS             5919..6947
                     /locus_tag="ACHWN2_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR8932886.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6999..7385
                     /gene="gpFI"
                     /locus_tag="ACHWN2_003496"
     CDS             6999..7385
                     /gene="gpFI"
                     /locus_tag="ACHWN2_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR8932887.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7397..7774
                     /locus_tag="ACHWN2_003497"
     CDS             7397..7774
                     /locus_tag="ACHWN2_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR8932888.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7761..>8225
                     /locus_tag="ACHWN2_003498"
     CDS             7761..>8225
                     /locus_tag="ACHWN2_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8932889.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1905 a         1933 c         2541 g         1846 t
ORIGIN      
        1 ctactcattg ccagatggga actgaccgca gaacaggctg tcgcacagca gttaaaaaat
       61 caggtgtcaa aaggaaatct tatcgatacc ggattttgta tttttgccct tagtaagctg
      121 gcgatggctt tatccagtac gcttgactcc attcctttgt ccatgcaacg acagttcccg
      181 gatttaacgc cgcgtcatat tgatcatctg aaaatcctta ttgctaaggg agcgaatcag
      241 tgcgcgcgtg cgggggataa attaccggat ttactcgatg aatatatcag aacaacaact
      301 gagtaatatg atggtggcag tcactatcgc actgcagcca ctggtcaggg tattgccggt
      361 gacggctgta gaatgggccg atcaaaatta ttacctgcct aaagaatcgt catacggtga
      421 gggggaatgg aaaacgctgc agtttcaggt cgccatcatg aactgcatgg gcaatgacga
      481 gattcgtaca gttaacctga ttaaatctgc ccgtgttggt tatacaaaaa tggtgcttgg
      541 tgtgatcggg tattttattg agcacaaatc ccgcaacact cttctttttc agccgacgga
      601 ctcagctgct gaagatttta tgaagtctca cgtggaagca acaattcgtg atgttccttg
      661 cctgaaaaaa ctttctccct ggctgggccg gaaacaccgg gataatacgc tcacactgaa
      721 gcgtttttcc tctggtgtgg ggttctggtg tttgggtggt gctgctgcca aaaactaccg
      781 tgaaaaatct gtcgatgtgg tctgctatga cgaactctcc tcgtttgaac cggatgtgga
      841 aaaagaaggt tcgccaacac tgcttggcga taaacgtatt gaaggttcgg tatggccaaa
      901 atccatacgc ggttcaacgc caaaaattaa aggctcctgt cagattgaaa aagctgcgaa
      961 tgaatctgcg catttcatgc ggttttatgt cccttgccct cattgcgggg aggagcagta
     1021 tctgaagttt ggcgatgatg cgacgtcgtt tggcctgaaa tgggagaagg gtaaaccgga
     1081 aacggtgtat tacctgtgtg aacataatgg ctgcgtgatc cgtcagtctg aacttgatca
     1141 gaccgggggg cgctggattt gtgacaatac cgggatgtgg acgtgtgacg gtctgacgtt
     1201 tttcagtgct tcgggtaatg aaattccgcc gccgcgctcc atcactttcc atatatggac
     1261 ggcgtacagt ccgttcacca ccagggtaca gatagtctgt gactggctgg atgcactgaa
     1321 agatcccaac ggcgtgaaaa cttttgtgaa caccacgctg ggcgagacct gggaagaggc
     1381 cgtgggcgaa aaactcgatc accaggtact gatggataag gttgtgcctt acacggcaac
     1441 ggtgcctgtc cgggtggttt atctgacggc gggcattgac tcgcagcgaa accgttttga
     1501 gatgtatgtc tgggggtggg ctccgggaga ggaagccttt ctggtggata aaatcatcat
     1561 catgggacgt cctgatgagg aagagacgct gttacgtgtg gatgcggcga tcaataaaaa
     1621 ataccgccat gcagacggta ccgaaatgac catttcccgt gtctgctggg atatcggggg
     1681 gatcgatggc gaaatcgtgt atcagaggtc aaaaaaacac ggtattttcc gggtactgcc
     1741 ggtaaaaggt gcctctgtct atggcaagcc ggtgatcacc atgccgaaaa cccgcaatca
     1801 gcggggtgtg tatctgtgtg aggtggggac ggataccgca aaagaaattc tctatgcccg
     1861 aatgaaggcc gatcccaccc ctgtgaatga ggccacatcg tatgccatcc gctttcctga
     1921 taatccggag attttttcgc agacagaggc acaacaactg gtggcggagg aactggtgga
     1981 aaagtgggaa aaaggaaaga tgcgtctgtt gtgggataac aaaaagcggc gtaacgaagc
     2041 gctggactgc ctggtgtatg cctatgcggc attacgtgtg tccgtgcaac gctggcaact
     2101 tgatctggct gtgcagttgc atcccgcaag gatgcaactg cacaaccgac ccttgaagaa
     2161 ctggcagcga agctgtccgg aggagtgaat ggttacagtc gctgaactgc aggcgctgcg
     2221 tcaggcgcgc cttgatttat taaccggtaa acgggttgtg tcggtacaga aagacggtcg
     2281 caggatcgaa tatacggcag cgtccctgaa tgagcttaac cgtgcgatca acgatgcgga
     2341 gtcggttctg ggtacgaccc gacgccgccg accgctggga gtgaggttat gaaacgaacg
     2401 cctgtcctga ttgatgtgaa cggcgttccg cttcgtgaga gtctcagcta caacggaggc
     2461 ggcgcaggat ttggcgggca aatggcagag tggttaccac cggcgcagag tgccgatgcg
     2521 gccctgttgc ctgcattgcg tctggggaat gcccgggcag atgatctggt gcgcaataac
     2581 ggtatcgcgg ctaatgcggt ggccctgcat aaggatcata tcgtcgggca catgtttctt
     2641 atcagctacc gtctgaactg gcgttggctg gggatgcggg agaccacggc aaaaagtttt
     2701 gtcggggagg tggaagcagc ctggtcggaa tacgcagaag gaatgtttgg cgagatcgac
     2761 gtggaaggga aacgcacgtt tacggaattt atccgtgaag gggtgggcgt gcatgccttt
     2821 aacggcgaaa tctttgtgca gccggtctgg gatacggaaa ccacgcagtt attccgtacg
     2881 cgttttaaag ccgtgagtcc gaaacgggtg gacacgccag gacacggtat ggggaaccac
     2941 tttctgcggg ccggggttga ggtcgatcgg tatgggcggg cgattgcgta ccatatctgt
     3001 gaggatgatt ttccgttctc tggtgccgga cgctgggagc ggatcccgcg tgagcttccc
     3061 accgggcgtc cggccatgct gcatattttt gagccggtgg aggacgggca gacccgtggg
     3121 gctaatcagt tttacagcgt aatggagcgg atgaagatgc tggactccct gcaggccacg
     3181 cagcttcagt cggccattgt gaaggcgatg tatgcagcca caatcgaaag tgaacttgat
     3241 accgaaaagg catttgaata tatcgcgggg gcaccgcaag gtcagcagga taatccgctt
     3301 attaatattc tggagaagtt cacccgctgg tatgacacga ataacgtgac gctgggcggt
     3361 gttaaaattc cgcacctttt ttcctggcga tgcgctgaac ctgcagacag cgcaggattc
     3421 agacaatgga ttttcggcgc ttgaacaggc gctgctgcgg tatattgcag ccggtcttgg
     3481 cgtttcctac gaacagttgt cccgtgatta ctcgaaggtc agttactcaa gtgcccgcgc
     3541 ctccgccaat gagtcgtggc gctattttat ggggcggcga aaatttattg cggcccggct
     3601 ggccacgcag atgttttcct gctggctgga agaggcgctt cttcggggga ttattcgtcc
     3661 gccacgggcg cgttttgatt tttaccaggc gcgttcagcc tggtcacggg cagagtggat
     3721 tagctccgga agaatggcca ttgacgggct caaggaggtc caggaatcgg tgatgcgcat
     3781 tgaggccgga ctgagcacgt atgagaaaga actggcactg atgggcgaag attatcagga
     3841 cattttccgc cagcaggtca gggaatccgc agagcgcgaa aaagccggac tctcacgtcc
     3901 ggtgtggata gcccaggcgt atcagcagca gatagcggag agtcgcaggc cggaagagga
     3961 gacaacatca cgtgagacgt aatctttcac atattatcgc tgcggcattc aatgagccgc
     4021 tgcttctgga gcccgcctat gcgcgggttt tcttttgcgc gctcgggcgt gagatgggcg
     4081 cagcaagcct ttcggtaccg caacagcagg tacagcttga cgctcccggg atgctggctg
     4141 aaacggacga gtacatggcc ggaggcaaac gacctgcccg tgtttaccgg gtggtgaacg
     4201 gtattgctgt actgccggtg accggcacgc tggtacaccg gctgggtggc atgcggccat
     4261 tttccggaat gactggctat gacggcattg tcgcctgtct tcagcaggca atggcggaca
     4321 ctcaggtacg gggcgtactg ctggacattg acagttctgg cgggcaggcc accggcgcgt
     4381 ttgactgcgc tgacatgatt taccgtctcc ggcagctgaa gcctgtctgg gcactgtgca
     4441 atgacacggc ctgttcagcc gccatgttac tggcatcggc ctgctcccga cggctggtta
     4501 cccagacatc ccgtatcggc tccattgggg tgatgatggg ccatgtcagc tatgccggtc
     4561 atctggcaca ggccggtgtg gatatcacgc tgatttacgc cggatcgcac aaggtggatg
     4621 gcaatcagtt tgaatccctg ccagcaaagg ttcgccagga catgcagcag cgcattgatg
     4681 cggcgcgccg gatgtttgct gaaaaagtgg cgatgtatac cggtttatct gtggatgcgg
     4741 ttatgggaac agaggccgcc gtttttgaag gtcagtccgg tattgaggcc gaactggcgg
     4801 atgaattaat caatgcgtcg gacgccatca gcgtgatggc tgcgacgctg aacacacacg
     4861 atacaggagg cactatgccg caattaactg caacggaggc tgccgcgcag gagaaccagc
     4921 gagtgatggg gatcctgacg tgtcaggaag cgaaaggacg tgaacaactg gccacgatgc
     4981 tggcagggca acagggtatg agcgttgagc aggcccgggc gattctggcc gtggcggcac
     5041 cgcaacaatc tggtgtatct gcgcagagtg aagccgatcg cattatggca tgtgaagagg
     5101 cgaaaggacg tgaacaactg gcggcaacgc tggcggcgat gccggagatg acggtggaaa
     5161 aagcccgtcc gattctggct gcttcaccgc aggcgaatgc cggaccctca cttcgtgatc
     5221 agattatggc cctggatgag gcaaaagggg cagaggcgca ggctgaaaaa ctggcggcct
     5281 gtccggggat gaccgtggag aatgcccggg ctgtactggc ggcagcctca ggcagggcag
     5341 aaccggtttc tgcatccaca accgccatgt ttgaacattt catggcgaac cattcaccgg
     5401 cagcggtaca gggtggcgtg ccacagacgt cagcagacgg tgatgcggac gtgaaaatgc
     5461 tcatggccat gccatgaagt cagtgctgac catcaatatg aggtttttac aatatggtaa
     5521 cgaaaaccat cactgaacag cgtgcggaag tacgtatttt tgccggtaat gatccggccc
     5581 ataccgccac aggctgcagc gggatttcct cggcaacacc ggctctgacg cccctgatgc
     5641 tggatgagga taccgggaaa ctggtggtct gggacggaca gaaggcaggc catgtggttg
     5701 gcatactggt actgccgctt gaaggtacag agacggtgct gacgtattac aagtcgggga
     5761 cctttgcgac ggaggccatc cactggcctg agagagtgga tacacacaaa aaggcgaatg
     5821 cctttgccgg cagtgccctg agtcacgcag cgctgccgta acattttatc aggtcactgc
     5881 ggtagcctga ctgatttctg aatgaaagga actgatttat gggattgttt acgacccgcc
     5941 agttactcgg ttataccgaa caaaaagtta aatttcgtgc gctgtttctg gagctgtttt
     6001 tccgccgtac ggtgaatttc cataccgaag aggtaatgct ggacaaaatt accggaaaaa
     6061 cgccggtggc ggcctatgtc tccccggttg ttgaaggaaa ggtgttgcgt caccgtggcg
     6121 gtgaaactcg tgtactgcgc ccgggctacg ttaagccgaa acacgaattt aattaccagc
     6181 aggcggtgga gcgtcttccc ggtgaagatc cggcccagct gaacggcccg gcttaccgtc
     6241 gcctgcgtat catcaccgat aacctcaaac aggaagagca cgccattgtc caggtggaag
     6301 agatgcaggc ggtgaatgcc gtgctgtatg gcaaatacac catggaagga gagcagtttg
     6361 aaactgttga ggtcgatttt ggccggtcgg tgggaaataa cattacgcag gcaggaggga
     6421 cggagtggag cgctcaggac cgcgatacct ttgatccgac gcatgatctg gatgcgtact
     6481 gtgattttgc ttccggcacc atcaatattg ccattatgga tggtaaggtc tggcgtctgc
     6541 tgaatggctt taagctgttc cgcgaaaaac tggatacccg tcgtggctca aattcacagc
     6601 tggaaacggc agtgaaagac ctgggggcgg tggtgtcctt caaagggtat tatggtgatc
     6661 tggcaatcgt ggtggcaaaa acgtcctatg tggcagagga cggtaccgaa aaacgttatc
     6721 tgccggacgg cacactggta ctgggaaata cggccgcaga gggtattcgt tgctacggtg
     6781 ccattcagga tgcgcaggca ttgtctgaag gcgtggtggc gtcttcccgt tatccgaaac
     6841 actggctgac cgtgggtgat ccggcgcgtg aattcaccat gacgcagtcc gcgccgctga
     6901 tggtgctgcc ggatccggat gagtttgtgg tggtgcaggt gaaataatcc gtgagcggga
     6961 gctaaatgct cccgtttttc attcaatcag gagcgggtat ggctacgaaa gaagaaaacc
     7021 tgaaacgtct ttgtgagctg gctgagaggc tggggcgtga accggatgtc tccggtagtg
     7081 cggcagatat tgcgcagcga gtggcggaac tggaggaaga gcttggagat gcgggggagg
     7141 tcgcagagcc ggatacgtac tcaccatcga aagatgatcc gaccgcgcat aaaggcgagt
     7201 cagttcctga acagccaggc attgttaccg gagacgaaac ggggcaggtt acggtggtgg
     7261 cactggcgac actgcataca aaatgtaatg gttcagtgat atttgtttca ccgggaacgt
     7321 cgtttcgtgt ttctgctgag gtggctgcca gcatggcggc aggtggtctg gcaaaaaggc
     7381 agtaacagga gatgtcgtgg acggtttcga taatctgttt gatgctgcgc ttgccggtgt
     7441 caatgaagtc attctccgtg atatggggat cagcgccgtc attacgtccg gagaacttga
     7501 ggggacacac ctgacagggg tttttgatga tcccgaaagt atttcttttg ttgccggggg
     7561 gatccggctt gaggattcct caccgtgcct gtttgtgaaa acggcagata tatcgcagtt
     7621 acgccgtcag gatacgttaa ctatcgggga tgattctttc tttgtggatc gtattactcc
     7681 ggatgacggg ggatgctgtt atatccggtt aaggcggggc agtcctgccc cacagaataa
     7741 tgccagaatg aggcgatatg atgaagggac ttgagaatgc catccgcaat ctgaacagcc
     7801 tcgatacccg catggtgcca cagaccagcg catgggcggt aaaccgtgtg gcggcaaaga
     7861 ttgtttctgt cgccacacgg caggttgcgc agaataccgt tgccggggat aaccaggtga
     7921 aaggtattcc cctgaaaacg gtcagggagc gtgtcaggct gcttaaagcc agcccctcag
     7981 gaaaaatgta tgcccgcatg cgtgttaacc ggggtaacct gcccgccatt aagctgggta
     8041 ccgcacaggt cagactggcc cgctcccggc atggcagcaa ctcacgtcat cgtggcagcg
     8101 tactgaaagt ggggaaatac cttttccggg atgcgtttat tcagcaactg gcgaatggtc
     8161 gctggcatgt gatgcggcgt attgacggca aaaatcgtta ccccattgat gtggtgaaaa
     8221 tcccg
//
LOCUS       DAZSNO010000168         8181 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZSNO010000168 DAZSNO010000000
VERSION     DAZSNO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.170"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="ACHWN2_003499"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="ACHWN2_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHWN2_003500"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHWN2_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR8932890.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHWN2_003501"
     CDS             complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHWN2_003501"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR8932891.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHWN2_003502"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHWN2_003502"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR8932892.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="ACHWN2_003503"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="ACHWN2_003503"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR8932893.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="ACHWN2_003504"
     tRNA            7783..7858
                     /locus_tag="ACHWN2_003504"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
     gene            8016..8091
                     /locus_tag="ACHWN2_003505"
     tRNA            8016..8091
                     /locus_tag="ACHWN2_003505"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8049..8051,aa:Gly,seq:gcc)
BASE COUNT         1870 a         2109 c         2222 g         1980 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagctc
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagata
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag attaagcggg aatagctcag ttggtagagc
     8041 acgaccttgc caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aaaatttaaa
     8101 agtgctgtaa ggcatagacc acccaagcgg gaatagctca gttggtagag cacgaccttg
     8161 ccaaggtcgg ggtcgcgagt t
//
LOCUS       DAZSNO010000169         8162 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZSNO010000169 DAZSNO010000000
VERSION     DAZSNO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.171"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(135..755)
                     /gene="sodA"
                     /locus_tag="ACHWN2_003506"
     CDS             complement(135..755)
                     /gene="sodA"
                     /locus_tag="ACHWN2_003506"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR8932894.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            1040..2074
                     /gene="rhaT"
                     /locus_tag="ACHWN2_003507"
                     /pseudo
     CDS             1040..2074
                     /gene="rhaT"
                     /locus_tag="ACHWN2_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(2071..2919)
                     /gene="rhaR"
                     /locus_tag="ACHWN2_003508"
     CDS             complement(2071..2919)
                     /gene="rhaR"
                     /locus_tag="ACHWN2_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR8932895.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(2993..3829)
                     /gene="rhaS"
                     /locus_tag="ACHWN2_003509"
     CDS             complement(2993..3829)
                     /gene="rhaS"
                     /locus_tag="ACHWN2_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR8932896.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            4117..5586
                     /gene="rhaB"
                     /locus_tag="ACHWN2_003510"
     CDS             4117..5586
                     /gene="rhaB"
                     /locus_tag="ACHWN2_003510"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR8932897.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            5583..6801
                     /gene="rhaA"
                     /locus_tag="ACHWN2_003511"
                     /pseudo
     CDS             5583..6801
                     /gene="rhaA"
                     /locus_tag="ACHWN2_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
     gene            7106..7867
                     /gene="rhaD"
                     /locus_tag="ACHWN2_003512"
     CDS             7106..7867
                     /gene="rhaD"
                     /locus_tag="ACHWN2_003512"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR8932898.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            7877..>8162
                     /gene="rhaM"
                     /locus_tag="ACHWN2_003513"
     CDS             7877..>8162
                     /gene="rhaM"
                     /locus_tag="ACHWN2_003513"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR8932899.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSS"
BASE COUNT         1932 a         2125 c         2187 g         1918 t
ORIGIN      
        1 aacgctgttt tatttttata ggcccattgt ttttattttg gaattgatca caaaaaaacc
       61 accgccgttg gcgatggttc attttttaag ctgatatgcg gcctatacgc ctcattgcag
      121 caggcggcaa atgattattt tttcgccgca aaacgtgccg ctgcttcgtc ccagttcacc
      181 acgttccaga actctttgat gtagtccgga cggcggttct ggaatttcag gtagtaagca
      241 tgttcccaca catccaggcc cagaatcggg aagccggaag cgccagaaat agcttcaccc
      301 atcagcggag aatcctggtt agcagtagaa accaccgcca gtttatcgcc tttcagcacc
      361 agccatgccc agccggaacc aaagcgggaa gctgccgctt tttcaaattc tgctttgaag
      421 ttatcaacgg agccgaagtc acgttcgata gccgctttca ggtcaccctg cagggtggtg
      481 ccttttttca gacctttcca gaacaggctg tggttagcgt gaccgccagc gttgttgcgc
      541 agtacggttt tcttgtctgc tggcagctgg tccagtttgg tgatcagctc ttcaaccggc
      601 aggttggcaa attctggcag gctttccagc gccgcgttgg cgttgtttac gtaggtctga
      661 tggtgtttgg tgtggtggat ttccatggtc tgcttatcga agtgcggttc cagggcatcg
      721 taagcatacg gcagggatgg cagggtatag ctcatattca tctccagtat tgtcgggcgg
      781 ccgattgtta atgccgcgta agcagttggt tcattatagt taattaaatg atattgaaaa
      841 tgattatcaa tgccgtactt ttcgtaaggg tatggttttg caggaaaatg cccgagatgt
      901 gaagcaaatc acccacttaa tgccgtgatt gccagtaaat cgacaacggc ggcaacaggc
      961 gaaaggttaa tcgacagcac gatttttaca ctcatctcgt cggagatgtg aagcgacgaa
     1021 aaatgatgag gataagaaga tgagtaacgc gattacgatg gggatatttt ggcatttgat
     1081 cggcgcggcc agtgcagcct gtttttacgc tccgttcaaa aaagtaaaaa aatggtcatg
     1141 ggaaaccatg tggtcagtcg gtgggattgt ttcgtggatt attctgccgt gggccatcag
     1201 cgccctgtta ctaccggatt tctgggcgta ttacagctcg tttagtctct ctacgctgct
     1261 gcctgttttt ctgttcggcg ctatgtgggg gatcggtaat atcaactatg gcctgaccat
     1321 gcgttatctc ggcatgtcga tgggaattgg catcgccatt ggcattacgt tgattgtcgg
     1381 tacgctgatg acgccaatta tcaacggcaa tttcgatgtg ctgattaaca ccgaaggcgg
     1441 acgcatgacg ttactcggcg ttctggtggc gctgattggc gtagggattg taactcgagc
     1501 cgggcagttg aaagagcgca agatgggcat taaagccgaa gagttcaatc tgaaaaaagg
     1561 gctggtgctg gcggtgatgt gcggcatttt ctctgccggg atgtcctttg cgatgaacgc
     1621 cgcaaaaccg atgcatgaag ccgctgccgc acttggcgtc gatccactgt atgttgctct
     1681 gccaagctat gttgtcatca tgggcggcgg cgcgatcatt aacctcggtt tctgttttat
     1741 tcgtctggca aaagtgaagg atttgtcgct aaaagccgac ttctcgctgg caaaaccgct
     1801 gatcattcac aatgtgttac tctcggcact gggcgggttg atgtggtatc tgcaattctt
     1861 tttctatgcc tagggccacg cccgcattcc ggcgcagtat gactacatca gttggatgct
     1921 gcatatgagt ttctatgtat tgtgcggcgg tatcgtcggg ctggtgctga aagagtggaa
     1981 caatacagga cgccgtccgg taacggtgtt gagcctcggt tgtgtggtga ttattgtcgc
     2041 cgctaacatc gtcggcatcg gcatggcgaa ttaatctttc tgcgaattga gatgacgcca
     2101 ctggctgggc gtcatcccgg tttcccgggt aaacaccacc gaaaaatagt tactatcttc
     2161 aaagccacat tcggtcgaaa tatcactgat taacaggcgg ctatgctgga gaagatattg
     2221 cgcatgacac actctgacct gccgcagata ttgattgatg gtcattccag tctgctggcg
     2281 aaattgctga cgcaaaacgc gctcactgca cgatgcctca tcacaaaatt tatccagcgc
     2341 aaagggactt ttcaggctag ccgccagccg ggtaatcagc ttatccagca acgtttcgct
     2401 ggatgttggc ggcaacgaat cactggtgta acgatggcga ttcagcaaca tcaccaactg
     2461 cccgaacagc aactcagcca tttcgttagc aaacggcaca tgctgactac tttcatgctc
     2521 aagctgaccg ataacctgcc gcgcctgcgc catccccacg ctacctaagc gccagtgtgg
     2581 ttgccctgcg ctggcgctaa atcccggaat cgctccctgc cagtcaagat tcagcttcag
     2641 ccgctccggg caataaataa tattctgcaa aaccagatcg ttaacggaag cgtaggagtg
     2701 tttatcgtca gcatgaatat aaaagagatc gccacgggta atgcgataag gacgatcgtt
     2761 gagtacatgc agaccattac cgcgccagac aatcaccagc tcacaaaaat catgtgtatg
     2821 ttcggcaaag acatcttgcg gataacggtc agccacagcg actgcctgct ggtcgctggc
     2881 aaaaaaatca tctttgagaa gttttaactg atgcgccacc gtggctacct cggccagaga
     2941 acgaagttga ttattcgcaa tatggcgtac aaatacgttg agaagattcg cgttattgca
     3001 gaaagccatc ccgtccctgg cgaatatcac gcggtgacca gttaaactct cggcgaaaaa
     3061 gcgtcgaaaa gtggttactg tcgctgaatc cacagcgata ggcgatgtca gtgacgctgg
     3121 cctcgctgtg gcgtagtaga tgtcgggctt tcatcagtcg caggcggttc aggtatcgct
     3181 gaggcgtcag tcccgtttgc tgcttaagct gccgatgtag cgtacgcagt gaaagagaaa
     3241 attgatccgc cacggcatcc caattcacct catcggcaaa atggtcctcc agccaggcca
     3301 gaagcaagtt gagacgtgat gcgctgtttt ccaggttctc ctgcaaactg cttttacgca
     3361 gcaagagcag taattgcata aacaagatct cgcgactggc ggtcgagggt aaatcatttt
     3421 ccccttcctg ctgttccatc tgtgcaacca gctgtcgcac ctgctgcaat acgctgtggt
     3481 taacgcgcca gtgagacgga tactgcccat ccagctcttg tggcagcaac tgattcagcc
     3541 cggcgagaaa ctgaaatcga tccggcgagc gatacagcac attggtcaga cacagattat
     3601 cggtatgttc atacagatgc cgatcatgat cgcgtacgaa acagaccgtg ccaccggtga
     3661 tggtataggg ctgcccatta aacacatgaa tacccgtgcc atgttcgaca atcacaattt
     3721 catgaaaatc atgatgatgt tcaggaaaat ccgcctgcgg gagccggggt tctatcgcca
     3781 cggacgcgtt acaagacgga aaaaaatcca cactatgtaa tacggtcata ctggcctcct
     3841 gatgtcgtca acacggcgaa atagtaatca cgaggtcagg ttcttacctt aaattttcga
     3901 cggaaaacca cgtaaaaaac gtcgattttt caagatacag cgtgaatttt caggaaatgc
     3961 ggtgagcatc acatcaccac aattcagcaa attgtgaaca tcatcacgtt catctttccc
     4021 tggttgccaa tggcccattt tcctgtcagt aacgagaagg tcgcgaattc aggcgctttt
     4081 tagactggtc gtaatgaaat tcagcaggat cacattatga cctttcgcaa ttgtgtcgcc
     4141 gtcgatctcg gcgcatccag tgggcgcgtg atgctggcgc gttacgagcg tgaatgccgc
     4201 agcctgacgc tgcgcgaaat ccatcgtttt aacaatgggc tgcatagcca aaacggttat
     4261 gtcacctggg atgtggatag cctggaaagt gccattcgcc ttggattaaa caaggtgtgc
     4321 gaggaaggga ttcgtatcga tagcattggg attgatacct ggggcgtgga ctttgtgctg
     4381 ctcgaccaac agggtcagcg tgtgggcctg cccgttgctt atcgcgatag ccgcaccaat
     4441 ggcctgatgc cgcaggcaca acagcaactc ggcaaacgcg atatttatca gcgtagcggc
     4501 atccagtttc tacccttcaa tacgctttat cagttgcgtg cgctgacgga gcaacaacct
     4561 gaacttattc cacacattgc tcacgctctg ctgatgccgg attacttcag ttatcgcctg
     4621 accggcaaga tgaactggga atataccaat gccaccacca cacaactggt caatatcaat
     4681 agcgacgact gggacgagtc gctactggcg tggagcgggg ccaacaaagc ctggtttggt
     4741 cgcccgacgc atccgggtaa tgtcattggg cactggattt gcccgcaggg taatgagatt
     4801 ccggtggtcg ccgttgccag ccatgatacc gccagcgcgg ttatcgcctc gccgttaaac
     4861 ggttcacgcg ccgcttatct ctcttctggc acctggtcat tgatgggctt cgaaagccag
     4921 acgccattta ccaatgacac ggcgctggca gccaacatca ccaatgaagg cggggcggaa
     4981 ggtcgctatc gggtgctgaa aaatattatg ggcttatggc tgcttcagcg agtgcttcag
     5041 gaacggcaaa tcaacgatct cccggcgctt atcgccgcga cacaggcact tccggcctgc
     5101 cgcttcatca tcaatcccaa tgacgatcgc tttattaatc ctgacgagat gtgcagcgaa
     5161 attcaggctg cgtgtcggga aacggcgcaa ccgatcccgg gaagtgatgc tgaactggcg
     5221 cgctgtattt tcgacagtct ggcgctgctg tatgccgatg tgttgcatga gctggcgcag
     5281 ctgcgcggtg aagatttctc gcaactgcat attgtcggcg gcggctgcca gaacacgctg
     5341 ctcaaccagc tatgcgccga tgcctgcggt attcgggtga tcgccgggcc tgttgaagcc
     5401 tcaacgctcg gcaatatcgg catcgagtta atgacgctgg atgaactcaa caatgtggat
     5461 gatttccgtc aggtcgtcag caccaccgcg aatctgacca cctttacccc taatcctgac
     5521 agtgaaattg cccactatgt ggcgcagatt cactctacac gacagacaaa ggagctttgc
     5581 gcatgaccac tcaactggaa caggcctggg agctggcgaa acagcgtttc gcggcggtgg
     5641 ggattgatgt cgaggaggcg ctgcgccaac ttgatcgttt acccgtttca atgcactgct
     5701 ggcagggcga tgatgtttcc ggttttgaaa acccggaagg ttcgctgacc ggggggattc
     5761 aggccacagg caattatccg ggcaaagcgc gtaatgccag tgagctacgt gccgatctgg
     5821 aacaggctat gcggctgatt ccggggccga aacggcttaa tttacatgcc atctatctgg
     5881 aatcagatac gccagtctcg cgcgaccaga tcaaaccaga gcacttcaaa aactgggttg
     5941 aatgggcgaa agccaatcag ctcggtctgg attttaaccc ctcctgcttt tcgcatccgc
     6001 taagcgccga tggctttacg ctctcgcatc ccgacgacag cattcgccag ttctggattg
     6061 atcactgcaa agccagccgt cgcgtttcgg cctattttgg cgagcaactc ggcacaccat
     6121 cggtgatgaa catctggatc ccggatggta tgaaagatat caccgttgac cgtctcgccc
     6181 cgcgtcagcg tctgctggca gcactggatg aggtgatcag cgagaagcta aaccctgcgc
     6241 accatatcga cgccgttgag agcaaattgt ttggcattgg cgcagagagc tacacggttg
     6301 gctccaatga gttttacatg gggtatgcca ccagccgcca gactgcgctg tgcctggacg
     6361 ccgggcactt ccacccgact gaagtgattt ccgacaagat ttccgccgcc atgctgtatg
     6421 taccgcagtt gctgctgcac gtcagccgtc cggttcgctg ggacagcgat cacgtagtgc
     6481 tgctggatga tgaaacccag gcaattgcca gtgagattgt gcgtcacgat ctgtttgacc
     6541 gggtgcatat cggccttgac ttcttcgatg cctctatcaa ccgcattgcc gcgtgcgttg
     6601 ctggaaccta ccgctgagct gcgcaagctg gaagcggcgg gcgattacac tgcgcgtctg
     6661 gcactgctgg aagagcagaa atcgttgccg cggcaggcgg tctgggaaat gtattgccaa
     6721 catcacgata cgccagcagg tagcgaatgg ctggagagcg tgcgggctta tgagaaagcg
     6781 attttgagcc agcgtgggta aacactgccg gatgcggcac gagcgcctta tccggcctac
     6841 gggtcgaatg tccatgcggg ctgtcaccct caccccggcc ctctccctga aagggcgagg
     6901 gagaaaaaca tagcctcgtg cggcaacgga gctgaacacc gctccggaca gtcccctcgc
     6961 ccctacgggg agagggttag ggtgagggga acaagccgca ccatgtctaa acaaggcaac
     7021 ctaatttaca ggaacacaga acatgcaaaa cattacccag tcctggtttg tccagggaat
     7081 gatcaaagcc accaccgatg cctagctgaa aggctgggac gagcgcaacg gcggcaacct
     7141 gacgctgcgc ctggatgacg ccgatatcgc gctttatcac gacaacttcc acccacaacc
     7201 gcgctatatt ccgctcagcc aacccatgcc tttactggca aatacgccgt ttattgtcac
     7261 cggctccggc aaattcttcc gtaacgtcca gctcgatcct gcagctaact taggcgtcgt
     7321 aaaagtcgac agcgatggcg cgggctacca tattctctgg gggttaacca acgaagccgt
     7381 tccaacttct gaacttccgg ctcactttct ctcccactgc gagcgcatta aagccaccaa
     7441 cggtaaagat cgggtgatca tgcactgcca cgccaccaac ctgatcgccc tcacctatgt
     7501 actggaaaac gacaccgcgg tctttactcg ccaactgtgg gaaggcagca ccgaatgtct
     7561 ggtagtattc ccggatggcg ttggcattct gccgtggatg gtgcccggca cggacgaaat
     7621 cggccaggcg accgcacaag agatgcaaaa acattcgctg gtgttgtggc ccttccacgg
     7681 tgtgttcggt tccggaccga cgctggatga agcctttggt ttaatcgaca ccgcagaaaa
     7741 atcagcacaa gtattagtga aggtttattc gatgggcggc atgaaacaga ccatcagccg
     7801 tgaagagttg atagcgctcg gcaagcgctt cggcgttaca ccactagcca gtgcgctggc
     7861 gctgtaagga gcaaacatga tccgcaaagc ctttgtcatg caggtaaacc ccgacgccca
     7921 cgaagagtat cagcgtcggc ataatcccat ctggccggaa ctggaagcag tgttgaaatc
     7981 tcacggtgtg cataactacg ccatttacct cgacaaagcg cgtaatctgc tgtttgccat
     8041 ggtagagatt gaatctgaag aacggtggaa tgcggtagcc agtactgatg tttgccaacg
     8101 ttggtggaaa tatatgaccg atgttatgcc cgctaaccca gataacagcc cggtgagtag
     8161 cg
//
LOCUS       DAZSNO010000170         8155 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZSNO010000170 DAZSNO010000000
VERSION     DAZSNO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.172"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="ACHWN2_003514"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="ACHWN2_003514"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR8932900.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSSRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="ACHWN2_003515"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="ACHWN2_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR8932901.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHWN2_003516"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHWN2_003516"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR8932902.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTLSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="ACHWN2_003517"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="ACHWN2_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR8932903.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="ACHWN2_003518"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="ACHWN2_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR8932904.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHWN2_003519"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHWN2_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR8932905.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHWN2_003520"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHWN2_003520"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR8932906.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2054 a         2088 c         2073 g         1940 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcagtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cagggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaata atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaa
//
LOCUS       DAZSNO010000171         8150 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZSNO010000171 DAZSNO010000000
VERSION     DAZSNO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.173"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..104)
                     /locus_tag="ACHWN2_003521"
     CDS             complement(<1..104)
                     /locus_tag="ACHWN2_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8932907.1"
                     /translation="MSRKTRRYSKEFKAEAVRTVLENQLSISEDASRL"
     gene            149..328
                     /locus_tag="ACHWN2_003522"
                     /pseudo
     CDS             149..328
                     /locus_tag="ACHWN2_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(337..735)
                     /locus_tag="ACHWN2_003523"
     CDS             complement(337..735)
                     /locus_tag="ACHWN2_003523"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR8932908.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(735..962)
                     /gene="vapB"
                     /locus_tag="ACHWN2_003524"
     CDS             complement(735..962)
                     /gene="vapB"
                     /locus_tag="ACHWN2_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR8932909.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            1044..6297
                     /gene="traI"
                     /locus_tag="ACHWN2_003525"
                     /pseudo
     CDS             1044..6297
                     /gene="traI"
                     /locus_tag="ACHWN2_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6317..7063
                     /gene="traX"
                     /locus_tag="ACHWN2_003526"
     CDS             6317..7063
                     /gene="traX"
                     /locus_tag="ACHWN2_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR8932910.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7118..7678
                     /locus_tag="ACHWN2_003527"
     CDS             7118..7678
                     /locus_tag="ACHWN2_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR8932911.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7809..8021
                     /locus_tag="ACHWN2_003528"
     CDS             7809..8021
                     /locus_tag="ACHWN2_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR8932912.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1855 a         2066 c         2614 g         1615 t
ORIGIN      
        1 aataatcggg aagcgtcttc actgatcgaa agttgatttt caagaaccgt tctgacagct
       61 tcggctttga actctttaga gtaacgtcgg gtttttctgc tcattattag ctccttctga
      121 tgccattcta tttcaggaag gagtgtccgt taaactcagg ctacctcaga tcagtgaatc
      181 cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc atccggacac
      241 ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg aacgcgggga
      301 ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt cagttctcag
      361 gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc cccgactgcg
      421 ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac gggcaagttc
      481 tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa catcaatgcg
      541 ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt tttctgcacc
      601 atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt tcaggttaaa
      661 acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt tggtatcgag
      721 cataaacttc agcatcagaa tgactccctt tcttgcatgc cgggctgttc cctgttatcc
      781 ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc tccggctggc
      841 gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt ttccggcaat
      901 gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac ggtggtttcc
      961 attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat tgataccggg
     1021 atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg gccggaagtg
     1081 ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg ggagaacgct
     1141 gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag gatgtcttta
     1201 cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg caggatggca
     1261 gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt atctccatga
     1321 tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc gtggattttg
     1381 ctgtccgtca ggtggaggcc agtgcctcca cctgacggac ggacagtcag aaacggtgct
     1441 gaccggtaat ctggtgatgg cactgtttaa ccacgacacc agtcgcgatc aggaaccaca
     1501 gttacacacg catgcggtgg tgactaatgt tacgcagtat aacggcgaat ggaagacact
     1561 gagcagtgac aaagtgggga aaacggggtt cattgagaat gtgtacgcta atcagattgc
     1621 ctttggcagg ctctaccggg aaaaactgaa agagcaggtt gaggcgctgg gctatgaaac
     1681 tgaagtggtc ggtaagcacg gtatgtggga aatgccgggt gtaccggtgg aggccttttc
     1741 cggacgctca cagactatcc gggaggccgt cggggaagac gcctcgctga aatcccggga
     1801 tgtggcggcg ctggatacgc gtaaatccaa acagcacgtc gatcctgaag tcagaatggc
     1861 cgagtggatg cagacgctga aggaaaccgg gttcgacatc cgggcatatc gtgacgcagc
     1921 ggaacaacgg gcatataccc gcacgcagac acccggaccc gcttcacagg acgggccgga
     1981 tgtgcagcag gcggtgacac aggccattgc cggattaagt gaacgcaaag tgcagtttat
     2041 gtatacggac ctgctggcca ggacggtcgg catactgccg ccggaaaatg gtgtgattga
     2101 acgggcacgc gccggtatcg atgaggccat cagccgtgag cagcttatcc ccctcgatcg
     2161 tgagaagggg ctgttcacgt tcgggattca tatgctcgat gagctgtcag tccgggcact
     2221 cagtcgtgac atcatgaaac agaaccgggt gaccgtacat ccggagaaaa gtgtcccccg
     2281 gacggccggt tacagcgatg ccgtcagtgt tctggcacag gaccgtccgt cgctggccat
     2341 tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg gtggctgaac tggtcatgat
     2401 ggcccgggag caggggcggg aggtgcagat tatcgctgct gaccgtcgct cgcagatgaa
     2461 cctgaagcag gatgaacggc tgtccggtga actgataacc ggacgtcgtc agttgctgga
     2521 aggaatggcc ttcacgccgg gcagtaccgt tatcgtcgac cagggcgaaa aactctccct
     2581 gaaagagacg ttaaccctgc tggatggtgc cgcacgtcat aacgtacagg tcctgataac
     2641 cgacagcggg cagcgaaccg gtacaggcag tgcactgatg gccatgaagg atgccggggt
     2701 gaacacatat cgctggcagg ggggagaaca gcgaccggcc accatcatca gtgaaccgga
     2761 ccgtaatgtc ggctggccgg agattttgcg gccagcgtga aagccggaga agagagcgtg
     2821 gcacaggtca gcggagtacg ggaacaggcc atactgacac aggccattcg cagtgagctg
     2881 aaaacacagg gcgtgctcgg acacccggag gtaaccatga ccgccctgtc accggtctgg
     2941 ctggacagcc ggagccgtta tctgcgggat atgtaccgcc cgggaatggt gatggagcag
     3001 tggaacccgg agacacgcag tcatgaccgc tatgtgacag aacgtgtgac ggcgcagagt
     3061 cacagcctga ccctgcggaa tgcgcagggc gaaacgcagg tggtgcgtat ttcttccctg
     3121 gacagcagct ggtcgctgtt ccggccggaa aaaatgccgg tggcagacgg tgagcgtctg
     3181 agggtgacag ggaaaattcc cggactccgc gtctccggcg gtgaccgcct gcaggtggca
     3241 tccgtcagtg aagatgcgat gacggttgtt gtgccgggac gggctgaacc ggccaccctg
     3301 cctgtgagcg attcaccgtt cactgcactg aagctggaga acggctgggt ggaaacgccc
     3361 gggcattccg tcagcgacag tgcgacggtt tttgcctccg tcacacagat ggcaatggac
     3421 aatgccaccc tgaatggtct ggcccgcagt ggtcgtgatg tccggctgta ttcctcactg
     3481 gatgaaaccc gtactgcgga aaaacttgcc cgccatccct cctttacggt ggtttctgag
     3541 cagataaagg cgcgtgccgg tgagacattg ctggaaaccg ctatcagtct gcagaaagcc
     3601 gggcttcaca cgccggcaca gcaggccatt catctggccc ttcctgtgct ggaaagtaaa
     3661 aacctggcct tcagcatggt ggacctgctg acagaggcga agtcgtttgc tgcagaagga
     3721 accggttttg ctgacctggg aggggaaatc aatgcgcaga taaaacgggg tgatttactg
     3781 tatgtggatg tggcaaaagg ctatggcaca ggcctgctgg tttcccgtgc gtcgtatgag
     3841 gcagaaaaga gcattcttcg ccatattctc gaaggtaagg aggcggtcac gccgctgatg
     3901 gagagagtac ctggcgaact catggagaaa ctgacatcag gacagcgcgc cgccacccgt
     3961 atgatactgg aaacgtctga ccgtttcacg gtggtgcagg gctatgccgg tgtgggtaag
     4021 accacacagt tccgggcggt gatgtcagcc gtgaacatgc tgccggagag tgaacgtccc
     4081 cgtgtcgtgg ggctggggcc cacacaccgt gcggtcgggg agatgcgcag cgccggcgtg
     4141 gatgcacaga cactggcgtc ctttctgcat gacacgcagc tgcagcagcg cagcggagaa
     4201 actccggatt tcagcaacac gctgttcctt ctcgatgaga gctctatggt gggcaatacc
     4261 gacatggcac gggcatacgc cctgattgcg gccggtggcg gtcgggctgt ggccagcggt
     4321 gacacggacc agctgcaggc catcgcgccc ggtcagcctt tccgtctcca gcagacgcgc
     4381 agtgctgccg atgtggtcat catgaaggag attgtgcgtc agacgccgga actgcgggag
     4441 gcggtataca gcctgattaa ccgggatgtg gaaagggcgc tgtccgggct tgagcgtgtg
     4501 aaaccgtctc aggtgccacg tctggagggc gcatgggcac cggagcactc cgtgacggag
     4561 ttcagtcaca gccaggaagc gaaactggca gaagcgcagc agaaggcgat gctgaaaggc
     4621 gaggcttttc cggatgtccc catgacactg tatgaagcca ttgtccgcga ctataccggc
     4681 aggacaccgg aagcacggga gcagacgctg attgtcacgc acctgaatga ggaccggcgc
     4741 gtactgaaca gcatgattca tgatgcacgg gaaaaggccg gagaactggg gaaggtgcag
     4801 gtcatggtgc ctgtcctgaa cacagcgaat atacgcgacg gggagctgcg tcgtctctcc
     4861 acctgggaga ataacccgga tgcccttgcc ctggtggata atgtgtatca ccggattgcc
     4921 ggtatcagta aggatgacgg gctgataacc ctgcaggatg cggaaggtaa cacgcggctg
     4981 atttcgcccc gggaggcggt ggctgaaggc gtcacgctgt acaccccgga caccatccgg
     5041 gtggggaccg gtgacaggat acgcttcacg aagagtgacc gggagcgcgg ttatgtggcc
     5101 aacagcgtct ggacggtgac agcggtttcc ggtgacagtg tcacactttc tgacggtcag
     5161 cagacccgtg tgattcgccc cggtcaggag cgggcagagc aacatattga cctggcctat
     5221 gccatcaccg cccacggtgc acagggggcg agtgaaacct ttgccatcgc gcttgaaggc
     5281 acggaaggta accggaaact gatggccggc tttgagtcag cctacgtggc cctgtcgcgt
     5341 atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga tgccatcaac
     5401 aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga ccgggaggtc
     5461 atgaatgcag agcggctgtt cagtacggcg cgggagctgc gggacgtggc ggcaggccgt
     5521 gccgttctcc gtcaggcggg gctggccggg ggagacagtc ctgcacggtt tattgctccg
     5581 ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt ttgaccgtaa cggcaagtcc
     5641 gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg gggattcagc
     5701 ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg tggccctgca gggcagccgt
     5761 aacggagaaa gcctgctggc tgataatatg caggatggcg tccggattgc ccgtgataat
     5821 cctgacagtg gtgtggtggt aagaatcgcc ggtgaaggcc gtccgtggaa tcccggtgcc
     5881 atcaccggtg gccgcgtgtg gggggatatc ccggacaaca gcgtccagcc gggagccgga
     5941 aatggtgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga ggccatccgc
     6001 cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa caaacctgac
     6061 ctgccggacg gcaaaacaga gcaggctgtc agggaaattg ccgggcagga gcgtgaccgg
     6121 gctgccataa ctgaacggga agccgcgctg ccggagagtg tactgcgtga accacaacgg
     6181 gtgcgggaag cggtccggga agttgcccgg gaaaatttgc tgcaggagcg tctgcagcag
     6241 atggagcggg atatggttcg tgacctgcag aaagagaaaa ccccgggcgg agactgatac
     6301 aggaagacaa acactgatga caacggataa cacgaacacg acacgtaacg attcactggc
     6361 tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac agcgggatat
     6421 catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga tattccagct
     6481 gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctttttg ccctggtgtg
     6541 ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc ggctgtgggg
     6601 atggggaatt attgcccagt tcgcatatta tctggcaggc tttccttggt atgaggggaa
     6661 tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa cgcgcagtgg
     6721 gtggcgtaca gcagcggcca tactcctgat ggccctgtgg gggcctttgt ccggcaccag
     6781 ttacggtatt gccgggttgc tgatgctggc agtcagcaac cggctgtacc gggcggaaga
     6841 cagagcggaa cgtctggcgc tggtcgcctg cctgctggct gttattccgg cgcttaacct
     6901 tgccaccagt gatgcagcgg cggtggccgg tcttgtgatg acggtgctga ccgttggtct
     6961 ggtgttgtgt gccggaaaat cattaccccg tttctggccc ggagactttt tcccgacgtt
     7021 ctatgcctgt catctggctg tgctgggcgt tctggcgctg tgacgggtgt ggtatctttg
     7081 gccgcaagag gatgattcgt cagaggcaga acacagcatg acagagcaga agcgaccggt
     7141 actgacactg aagcggaaga cagagggaac agcgcctgtc cgcagccgga aaaccatcat
     7201 caatgtcacc acgccaccaa agtggaaggt gaaaaagcag aagctggccg agaaagcggc
     7261 ccgtgaagca gagctggcgg caaaaaaagc gcaggccaga caggcgctgt ccatttatct
     7321 gaacctgccc acgctggatg aggctgtgaa taccctgaag ccctggtggc cgggattatt
     7381 tgacggtgac acgccccggc ttctggcctg cggtatccgg gacgtgttac tggaagacgt
     7441 ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc agggcgctga aggccatcac
     7501 ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg acacggaagg
     7561 gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcgggagcgc gtctggcgaa
     7621 aattcgtcgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg agaaataaaa
     7681 aaagcctccc ccagagagag gaggcaggga aataaggctt aaaaggaaat aacttccgct
     7741 tccgaaagga agccagtcca ctgaattatt tatcacaata atactgacaa ctaaaggggg
     7801 acaagaaaat gaatggattc agaaacagtt cacggaacgg ccaggtctgg cgttaccagc
     7861 gtgccggcgg gcgggccgtt attctggaag tcagtggacg ctggatggaa gcggcagaag
     7921 catggcgacg ggctgcctgt gttgcgccta ggacagactg gcagcagttt gcccggaaaa
     7981 gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaaag cagccttcat
     8041 tactctagga gtcagaaggc tgaaaggagt ttatgagatt tgcttcttaa tatccggtcg
     8101 ggcagtatga ccggacagtt ctgagtagca cagagtcttt tccgcattaa
//
LOCUS       DAZSNO010000172         8114 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZSNO010000172 DAZSNO010000000
VERSION     DAZSNO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.174"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..223
                     /locus_tag="ACHWN2_003529"
     CDS             <1..223
                     /locus_tag="ACHWN2_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932913.1"
                     /translation="PVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETL
                     FSSKSHGKYALFPIFTASWRATRIKNKGI"
     gene            446..943
                     /gene="ubiC"
                     /locus_tag="ACHWN2_003530"
     CDS             446..943
                     /gene="ubiC"
                     /locus_tag="ACHWN2_003530"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR8932914.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            956..1828
                     /gene="ubiA"
                     /locus_tag="ACHWN2_003531"
     CDS             956..1828
                     /gene="ubiA"
                     /locus_tag="ACHWN2_003531"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR8932915.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1983..4406)
                     /gene="plsB"
                     /locus_tag="ACHWN2_003532"
     CDS             complement(1983..4406)
                     /gene="plsB"
                     /locus_tag="ACHWN2_003532"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR8932916.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4577..4945
                     /gene="dgkA"
                     /locus_tag="ACHWN2_003533"
     CDS             4577..4945
                     /gene="dgkA"
                     /locus_tag="ACHWN2_003533"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR8932917.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5055..5663
                     /gene="lexA"
                     /locus_tag="ACHWN2_003534"
     CDS             5055..5663
                     /gene="lexA"
                     /locus_tag="ACHWN2_003534"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR8932918.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5736..7061
                     /gene="dinF"
                     /locus_tag="ACHWN2_003535"
     CDS             5736..7061
                     /gene="dinF"
                     /locus_tag="ACHWN2_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR8932919.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7177..7386
                     /locus_tag="ACHWN2_003536"
     CDS             7177..7386
                     /locus_tag="ACHWN2_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR8932920.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7428..7943)
                     /gene="zur"
                     /locus_tag="ACHWN2_003537"
     CDS             complement(7428..7943)
                     /gene="zur"
                     /locus_tag="ACHWN2_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR8932921.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1825 a         1983 c         2222 g         2084 t
ORIGIN      
        1 gcctgttatg gtggtaagct cccagcgctt acatgatatg ttgaatccta caaaagatac
       61 caattggaat tccacgtgta tctataaatc cagacataag atgttgccta ttaatcttac
      121 tcaggaaaca cttttcagct ccaaatctca tggtaaatat gcgcttttcc caatttttac
      181 tgcgagttgg cgagctactc gtataaagaa taaaggtatt taagtaaagg aaaacatcac
      241 cgttcctagc atcatgggcg gtgatgcccc tatggttgcc ctcgccagca cgggcatcgg
      301 caaagcgtaa ggttcaacag cgttttacca cttcatgcga ttgttgcgtt tttgttgcgc
      361 attagatcac ttaatttgct ttacatctcc cgtaaacact tttctgcgat acaatgcctt
      421 tacgttatgt aacggagagt tcggcatgtc acaccccgcg ttaacgcaac tgcgtgcgct
      481 gcgctatttt aaagagatcc ctgccctgga tccgcaactg ctcgactggc tgttgctgga
      541 ggattccatg acaaaacgtt ttgaacagca gggaaaaacg gtgagtgtga cgatgatccg
      601 cgaggggttt gtcgagcaga atgaaatccc cgaagaactg ccgctgctgc cgaaagagtc
      661 tcgttactgg ttacgtgaaa ttttgttatg tgccgatggt gaaccgtggc ttgccggtcg
      721 taccgtcgtt cctgtatcaa cattaagcgg gccggaacta gcgttacaaa aattgggtaa
      781 aacgccgtta ggacgctatc tgttcacatc atcgacacta acccgggatt ttatcgagat
      841 aggccgtgat gccggactgt gggggcgacg ttcccgcctg cgattaagcg gtaaaccgct
      901 gttgctaaca gaactgtttt taccggcgtc accgttgtac taagaggaaa aaaatatgga
      961 gtggagtctg acgcagaata agctgctggc gtttcatcgc ttaatgcgta cggataagcc
     1021 aattggcgcg ttactgctgc tctggccaac attatgggcg ttgtgggtgg cgacaccggg
     1081 cgttcctcag ctctggatca tggcggtgtt tgtcgcgggt gtctggctga tgcgcgctgc
     1141 tggttgcgtg gtgaatgatt atgctgatcg caagtttgat ggtcatgtta agcgcacggc
     1201 gaaccgacca cttcccagcg gcgcggtaac agagaaagag gcgcgcgcgc tgtttgtagt
     1261 gctggtactg atttcgtttt tactggttct gacgctgaat acgatgacca ttctgttgtc
     1321 gattgccgct ctggcattgg cgtgggtgta cccgtttatg aagcggtata cccatctccc
     1381 gcaagtggtg ctgggcgcgg cgtttggctg gtcgattcca atggcttttg ccgctgtgag
     1441 tgagtcggtg ccattgagtt gctggttaat gttcctcgcc aatattctct gggcggtggc
     1501 ttacgacacg cagtatgcga tggttgaccg cgatgatgat gtgaagattg gcattaaatc
     1561 cacggcaatc ctgttcggcc aatacgataa attgattatt ggtattttgc agattggcgt
     1621 actggcactg atggcgatca tcggtgagtt aaatggctta ggctggggat attactggtc
     1681 aattgtggtg gctggcgcgc tgtttgttta tcaacaaaaa ctgattgcca accgcgagcg
     1741 tgaagcctgc tttaaagcat ttatgaataa taactatgtt ggtctggtac tgtttttagg
     1801 actggcaatg agttactggc atttctgatg atgtaaaaaa gccggatgat catccggctt
     1861 tcttctgggt tgcctgatgc gttgcgcttc tcaggcctac ccggtgaatc gcaatttatt
     1921 gaatttgcag gtcatggtag gccggataag gcgttttcgc cgcatccggc aattctctct
     1981 gattaccctt cgccctgcgt cgcactctca atcgtcaaac gcacgtctga tgtaatcaac
     2041 tccgccagca actgataaac cttcatcgtt tctgctggtt cggcatcgcc gctatcgctg
     2101 atataccctt catcacgcag cgtcagcacc agagaactga acaccgcctt gtcgaagaac
     2161 tccggcgcgt tgatgccgtg cagcacggag agacgttgcg caacggtgcg gctctctttc
     2221 tccagcgtac cgcggttgat cgacgggttg gcactcaaca accagaaggt gatggcataa
     2281 cgttgcagcg tttcgcgcgc gcctgcggcc agcagctgta gggtgcgaga atgcgccggg
     2341 ttgatatgca actcatcatc ttgcagggta atcagcccct gacgttgcat ctcatttgcc
     2401 agcgcatcaa taacgtccgg caactcgtcg cgatcccagc gcaggaacag ctccgctttc
     2461 agcattgggt aaagcacatt gacgtgctcc atcaatacgt cgcgggagat gtggcgatgc
     2521 tgggtgacaa ttgccgccat cagcgaaggc agcaccaaca tatgcgcaat gttgttgcga
     2581 tagtaggtca tcagcaccgc ttgctcgcgt ggcagaatga tgatgtcgcc gatagtgtct
     2641 ttctcgactt caaacttgtt catttgcagc gcgtgatcga taagctcgct ggcgctggct
     2701 gaaggaacgg tagagtccgt ggagtaaggt acgttgcgca tcagatccag gtagcagttg
     2761 agttgctcgg ttaactgctc gcgggtgagt gagcgctgac gtgatgccag tagcgcagta
     2821 cagcacaggt tcatggcgtt tgccgcgcct gcgttgttaa tgcgtaccat cagatcggca
     2881 gcaatattat tgaccgtcgg cgttaaccag gccggacgca ccgcttcgat gggatcgata
     2941 gattcacgcc agtctggtac gtgctggtta aggtaggtca tcaacggcat tggttcaccg
     3001 aagttgacgt aaccctgacc gagattacgc agcttgctta aaccgcgcag catctgcggc
     3061 aggctctctt tctctttcgt cgcgccgcgc agttctttgg cgtaagtacc cacttccatg
     3121 acgtgctcat aaccgatata gatcggaatc agcgtaatcg gacgcgtgcc gccacgcagc
     3181 atcgcctgaa tggtcatcga cagcgtaccg gttttcggat ccagcaaacg ccccgtgcgg
     3241 gaacgaccgc cttccacgaa gtactcgacg gaataaccac ggctgaacag ttcgccgaga
     3301 tactcacgga aaacggtgga ataaagttta ttgcctttaa aagtacggcg aataaagaac
     3361 gcccccagac ggcggaaaat cggcccggcc ggccagaagt tcaggttgat cccggcggcg
     3421 atatgcggcg gcaccagccc ctggtggtac agcacgtaag aaagtagcag gtagtccatg
     3481 tgactgcggt ggcaaggcac atacaccagc tcatgaccgt cgtgggccag ctggcgaacg
     3541 cgctcggcgt tatggacgtt gatgccctga taaaatcggt tccaggtgaa gcccagaatg
     3601 cggtcagtca ggcggatcat ctcgtaagag aaattcgccg caatctcttc catcagcgca
     3661 atcgcgttct gctgcgcttt ttcatgggag atttttttgc tgcgcgcttc atcttctacc
     3721 gctttggcaa tggcgcggga ggcgagcagc ttattaaaca gatcctgacg agcaggcaga
     3781 cgtgggccta cggcagccag acgttgacgg gcaaagtgca tacgcgccac gcgcgccagt
     3841 ttctgagcga tagttttatc cgtgccgtgt tcatccgcca tacggcgcag cgacactgac
     3901 ggcgagaaac gcacaaaact gtcgcgaccg agccacagta cagcgaaaaa tttctgtacg
     3961 ccgttaagca tacgcagcgg cgggttcact tcgccttttt cacgccccgg cgcgcgacca
     4021 aacatcaccg atactggcac catctgcaca tccagatttg ggttgctacg gtgcaaatcg
     4081 agatagtcgt ggaaaagctt aatagactct tctttcggcg tgtaataggt gaatacacgc
     4141 ggcccgccgt gaatgaacac atagcgcggc agtagcgtgc cgtcgatttc cagcggctct
     4201 aacgggtcag gcaaatcatg tgccagacac tgggcgcgca acgtcagcaa atctgctttc
     4261 gagttgtacg gtaaaacgta cataattgga cgagaggtat ccagccccag ttccggggca
     4321 ggatctgccg gaatagactt gctttttacc aggatgctta atggtaaatt cagtaatttg
     4381 tagtaaattc gtggccagcc ggacataaac gatgtaaagc ctctggttaa taatgcaaat
     4441 gcgcggcaag gatagcagaa agtcatggga aattctgtgg tatccgctca tgtttcgcgc
     4501 ggcgttacgc aaacccgaat catcggattt aacggtacac tgatattgac gctcataatg
     4561 taaaaaggtt ctttcaatgg ccaataatac aactggattc acccgaatta tcaaagctgc
     4621 tggctattcc tggaaaggtt tacgcgctgc atggatcaac gaagcggcat tccgtcagga
     4681 aggcgtagcg gtattgttgg cggtggtcat cgcctgctgg ctggatgtgg acgcaattac
     4741 ccgcgtgctg cttatcagct ccgtgatgct ggtgatgatt gtggaaatcc tcaatagcgc
     4801 catcgaagca gtggttgacc gaattggctc tgaataccat gagctttccg gacgcgcaaa
     4861 agatatggga tccgccgcgg tgctgattgc cattatcgtc gccgtgatta cctggtgcat
     4921 cctgttatgg tcgcattttg gataaccctt ccagatttcg ataaatctct ggtttattgt
     4981 gcagtttatg gttccaaaat cgccttttgc tgtatatact cacagtataa ctgtatatac
     5041 acccaggggg cggaatgaaa gcgttaacgg ccaggcaaca agaggtgttt gatctcatcc
     5101 gtgatcacat cagccagaca ggtatgccgc cgacgcgtgc ggaaatcgcg cagcgtttgg
     5161 ggttccgttc cccaaacgcg gctgaagaac atctgaaggc gctggcacgc aaaggcgtta
     5221 ttgaaattgt ttccggcgca tcacgcggga ttcgtctgtt gcaggaagag gaagaagggt
     5281 tgccgctggt aggtcgtgtg gctgccggtg aaccgcttct ggcgcaacag catattgaag
     5341 gtcattatca ggtcgatcct tccttgttca agccgaatgc tgatttcctg ctgcgcgtca
     5401 gcgggatgtc gatgaaagat atcggcatta tggatggcga cttgctggca gtgcataaaa
     5461 ctcaggatgt acgtaacggt caggtcgttg tcgcacgtat tgatgacgaa gttaccgtta
     5521 agcgcctgaa aaaacagggc aataaagtcg aactgttgcc agaaaatagc gagtttaaac
     5581 caattgtcgt tgaccttcgt cagcagagct tcaccattga agggctggcg gttggggtta
     5641 ttcgcaacgg cgactggctg taacatatct ctgagaccgc gatgccgctt ggcgtcgcgg
     5701 tttgtttttc atctctcttc atcaggcttg tctgcatggc attcctcact tcatctgata
     5761 aagcactctg gcatctcgcc ttacccatga ttttctccaa tatcaccgtt ccgttgctgg
     5821 gactggtcga tacggcggta attggtcatc ttgatagtcc ggtttatttg ggcggcgtgg
     5881 cggttggtgc aacggcgacc agctttctct ttatgctgtt gctgttttta cgcatgagca
     5941 ccaccgggct gactgcgcag gcttatggtg ccaaaaatcc tcaggcatta gcccgtgcgc
     6001 tggtgcaacc gttgctgttg gcgttggggg ccggggcgtt aattgcgctg ttgcgtacgc
     6061 cgattatcga tctggcgctg catattgttg gcggtagtga agcggtcctg gaacaggcgc
     6121 ggcgctttct tgaaatccgc tggttaagcg ctccggcgtc gctggcgaat ctggtattac
     6181 tcggttggtt actcggcgtg caatatgccc gtgcgccagt aattttgtta gtggtcggca
     6241 atatcctcaa cattgtgctg gatgtctggc tggtgatggg gctgcatatg aacgtgcagg
     6301 gcgcggcgct ggcgacggtt attgcggaat atgcaacatt gctgattggt ctgctaatgg
     6361 tgcgtaaaat cctcaaacta cgcggaattt ccggcgaaat gctgaaaact gcctggcgag
     6421 gaaacttccg tcgcttgctg gcgcttaacc gcgatatcat gctgcgttcg ctgttgttgc
     6481 aactctgttt cggcgcgatc accgtacttg gtgcgcgact ggggagtgac attatcgctg
     6541 ttaacgcggt tctgatgacg ctactcacct ttaccgccta tgcgcttgat ggttttgcct
     6601 acgcggttga agcgcactcc ggtcaggcat acggtgcgcg cgacggtagc cagttgctgg
     6661 atgtatggcg ggcagcgtgc cgccagtcgg ggattgtagc gttactattt tcggtggttt
     6721 atttgctggc aggggaacac atcattgcgt tgctgacgtc gttaacccag attcagcagc
     6781 tggctgaccg ctatcttatc tggcaggtga ttttgccgtt ggttggcgtc tggtgttatc
     6841 tgctggacgg catgtttata ggcgcaacgc gtgccgccga aatgcgtaac agtatggcgg
     6901 tggccgccgc aggttttgcg ctgacgctcc ttacgctgcc gtggctgggt aatcatggtt
     6961 tgtggctggc attaaccgtc tttctggcgt tgcgcgggct ttctctggcg gctatctggc
     7021 ggcgtcactg gcgcaacggt acctggtttg ccgcaacgtg acggttaaaa attctgaata
     7081 aattatccta agccaaattg ctgactacac ttaatctcac gttcagaaga aaagtgaacg
     7141 tactctcatt cacaacctaa cgatgaggtc ttgattatga ataaagatga agccggcggt
     7201 aactggaaac agtttaaagg taaagtgaaa gagcaatggg gcaaactgac cgatgatgat
     7261 atgacgatca ttgaaggtaa acgtgatcaa ctggtcggta aaatccagga acgttatggt
     7321 tatcagaaag atcaggcaga aaaagaggtc gtggattggg aaacccgcaa tgaatatcgc
     7381 tggtaattaa tccctcctgc ccgacgtgta caaggatgta cgccctctta acgcggtttc
     7441 tttttcacct gcacagagtg atcatgctgg cactgttcag gatgacgaca cgcttccact
     7501 tccacgcatg ccgcacagag cccatgtgct tcaatcacat tatgccgcag ggcaaacccc
     7561 atttttgccg ccagcgtatg cataatgtct tccacgcctt ctgcacactc ttctttcacc
     7621 gcgccgcagc gatcgcaaat aaacatggct gacgtatgcg tgggctggtc gaacagatga
     7681 cagagcacat aactgttggt ggattccacc ttatgcacaa aaccttgctc aagcagaaaa
     7741 tccagcgcgc gataaaccgt tggcggcttg gcttgcggtt cagcttcgcg cagtaaatca
     7801 agcagatcat aagcgctgat agcgccatcc tgcagactca tcaggcgcaa cacttccagg
     7861 cgctgtgggg tcaggcgcac attacgctgc gcgcagattt tttcagcctg cgctaataac
     7921 tcctgcgttg tggtcttttc catcgggcac ctcaaagtgg gggttaagaa tgtccttact
     7981 ttaccatgtt ccaggaaaaa caccgagaac ccttattgtt gccgtaatgt tgattttctg
     8041 ttttgtaggt aaggtgttgt gttgccttat cgtaccatta tcaacacgat aataattaat
     8101 aattcatttt taaa
//
LOCUS       DAZSNO010000173         7940 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZSNO010000173 DAZSNO010000000
VERSION     DAZSNO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.175"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(52..531)
                     /gene="xdhC"
                     /locus_tag="ACHWN2_003538"
     CDS             complement(52..531)
                     /gene="xdhC"
                     /locus_tag="ACHWN2_003538"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR8932922.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="ACHWN2_003539"
     CDS             complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="ACHWN2_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR8932923.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="ACHWN2_003540"
     CDS             complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="ACHWN2_003540"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR8932924.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4129..4884
                     /gene="actS"
                     /locus_tag="ACHWN2_003541"
     CDS             4129..4884
                     /gene="actS"
                     /locus_tag="ACHWN2_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR8932925.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4963..5036
                     /locus_tag="ACHWN2_003542"
     tRNA            4963..5036
                     /locus_tag="ACHWN2_003542"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4995..4997,aa:Gly,seq:ccc)
     gene            5171..6787
                     /locus_tag="ACHWN2_003543"
     CDS             5171..6787
                     /locus_tag="ACHWN2_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8932926.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKGCQDRLKGDSSGLLSYLE
                     PETSSLRPSVSLSVSSEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEILDYLDIQDVGKATRADMLRVREVLQLLPKNRKQRFKTMPLSDLLNRESKNDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARGAFSPEQVRQLLV
                     AAKAYSQKTAGKPYHYYVTALAAVTGARLNEVAQLQVKDIRVTEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLIPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGVDGDTV"
     gene            6771..7427
                     /locus_tag="ACHWN2_003544"
     CDS             6771..7427
                     /locus_tag="ACHWN2_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFR8932927.1"
                     /translation="MAIPFEAPEFEKEAFNCPYCNAFAHQKWQQLYYYYSSTGGLPVH
                     TSTCEHCEEKSFWFQRRLLIPFASTAELPNPDMPDNCKADYMEARSIVGLSPKGAAAL
                     LRLCLQKLMVHLGEPGNNINADIRSLVQKGLPVRIQQAADICRIVGNQAVHPGEISLD
                     DDPQLAHGLFKLLNIIVDDRITRPKEIEAMFQSMPEGPRQGIENQDRQAREQQQAANE
                     "
BASE COUNT         1987 a         1984 c         2046 g         1923 t
ORIGIN      
        1 ttaaacacat aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt
       61 tttctcgcaa tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt
      121 tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag
      181 cattgccgtg gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga
      241 tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt
      301 gcggatctct tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc
      361 gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag
      421 cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa
      481 aggcatcccg ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt
      541 ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc
      601 agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat
      661 tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca
      721 tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa
      781 ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg
      841 cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga
      901 aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca
      961 ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt
     1021 agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta
     1081 gcgacgttac ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga
     1141 tgacgttgag ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt
     1201 agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca
     1261 atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg
     1321 agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg
     1381 cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa
     1441 ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat
     1501 cttcaccgcg ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt
     1561 atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag
     1621 atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt
     1681 agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg
     1741 tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat
     1801 gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc
     1861 aaacgccggt gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg
     1921 ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg
     1981 tcgttcaacc agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag
     2041 catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc
     2101 aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc
     2161 ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac
     2221 catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt
     2281 gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca
     2341 ggtgttagag gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg
     2401 gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca
     2461 ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg
     2521 ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc
     2581 aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc
     2641 cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag
     2701 gtaagcgact ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc
     2761 ggtgttagac agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat
     2821 ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct
     2881 aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc
     2941 ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac
     3001 gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg
     3061 ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct
     3121 ttccatgtga caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc
     3181 ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc
     3241 gccgttatgg attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg
     3301 taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg
     3361 ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg
     3421 atcggcggta tcgcgcttgt tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg
     3481 aatatcaggc acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg
     3541 ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta
     3601 acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat
     3661 ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat
     3721 cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta
     3781 cgccagaaac gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac
     3841 agatatgata cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg
     3901 ataaaatcaa tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag
     3961 tcaggaactt cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat
     4021 gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga
     4081 ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga
     4141 cgcctgaata aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg
     4201 ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg
     4261 aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg
     4321 gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg
     4381 ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc
     4441 gcatcggtta caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt
     4501 tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc
     4561 aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga
     4621 aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac
     4681 agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa
     4741 agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc
     4801 ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg
     4861 cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg
     4921 ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg
     4981 tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccatgac
     5041 ctttcttaaa gtctactaac ttactgaatc ctaagggaca tggcttaaag ccttccgata
     5101 gttttactca ctgtaaccaa cactgtgact aactttgttg acgtagtgag cagacctaag
     5161 gtctcagtac atgctcaact caagaaccta cctttatcag cgtaacggtg tcttttacat
     5221 ccgtcttagg atgaagacca ctggtcgcct gaccgcttcg cttccttctc acaaccgtta
     5281 taaactagcg tcagtatcac tacggaccaa ggatagacgt accgctatgg ctcactcacg
     5341 gcacatcaag tcagcactta aagcaatcca cgcagataac cctaacgcct cttatgagga
     5401 gctacgggaa cacctgaaga ccattgtcga gtgggaactt agtgtcagcc gcgatgacct
     5461 gaacgacccg gagtcctacc agctttacgt tgaccagtac gatgatatta agtccaacct
     5521 tcgggaagct gtagcgactg agcgtctgac tgtagaccag catcgctata tcaatgacgt
     5581 tatcggcgta cttaaaggct gtcaggatag actcaagggc gatagctctg gcctgttgtc
     5641 ttaccttgag cctgagacca gtagtctaag accatctgtc tccctatctg tatcatcgga
     5701 gcctgaagtc cccgaaccta aagctctgac cctcgcgtct ctcattgagc agtacgagca
     5761 ggagaacgct cagaactgga agccagcgac cctaagcgag aaccgagcgt cgcactccac
     5821 gctaatcgag atattagact atctggatat tcaggatgtc ggcaaggcga cccgtgcaga
     5881 tatgctcagg gtccgtgaag tcctccagct gttaccgaag aaccgcaagc aacgctttaa
     5941 gaccatgccg ctgtctgacc tgctgaaccg ggagtctaag aatgactgtc tagatgtcgt
     6001 taccatcaac aataaatatc ttatcaagat ggctgctgtg tttaagtggg cggtacgcaa
     6061 cgacctgata gctaagaacc tgacagaagg tcttgaactg aaagtccctc agcgtaaagc
     6121 ctccgacgct cgtggcgcgt tcagtcctga gcaggtaagg caactactgg tcgccgctaa
     6181 agcgtactct cagaagaccg caggtaagcc ataccattat tacgtcaccg ctctagctgc
     6241 ggttacaggt gcgagactta acgaggtggc ccagcttcag gtcaaagaca tcagggtcac
     6301 tgaggccggg actgtgtata tccatataaa cgaagacgat agcagcctac cgggtaagag
     6361 catcaagaac gctcatagcg accgctgtgt tccgctagta gatggcgctt atggttttgt
     6421 attggctgac tttatgtcgc ttgtggaaga ccgtagaaag actgagggtg ataatgctat
     6481 ggtattcgat ggcctcaagc tgatgaagaa tggctacggt gagcaggtta gtaaatggtt
     6541 taaccggacg ttgataccta aagtccttgc tgaccgtagc ggcttagcgt ttcattcgtt
     6601 taggcataca gttgcgaccc agttaaaaca acatggggtc gagttagcgt atgctcaggc
     6661 gattatgggt catagctcag ggtctataac ttacgaccgt tacgcgaaag aggtcgaggt
     6721 agatagattg gttaatgtga tggcgggtgt ttataaggag actggagtag atggcgatac
     6781 cgtttgaggc acctgagttt gaaaaagaag cctttaactg cccatattgt aacgcttttg
     6841 cacatcaaaa atggcagcag ttgtactatt actattcaag tactggaggc ctccctgttc
     6901 atacttctac ctgtgagcat tgtgaagaaa aaagtttttg gtttcagcgc cgcttactta
     6961 taccgtttgc ttccacagcc gaactaccaa acccggacat gcccgacaac tgtaaggctg
     7021 actacatgga agccagaagc atcgttggcc tgtcacctaa aggggccgct gcactccttc
     7081 gtctatgcct tcagaagcta atggtccact taggcgaacc ggggaataac attaacgctg
     7141 acatccggtc actcgttcag aaaggactac ctgtacgcat ccagcaggca gcagacatct
     7201 gccgtattgt cggcaatcag gcggtccatc ccggagagat tagccttgat gatgacccgc
     7261 agttggctca tggcctcttt aagctgctga acattattgt cgatgaccga atcacgagac
     7321 cgaaagaaat tgaggctatg ttccagagta tgcctgaagg tccacgccaa ggtattgaga
     7381 atcaggacag acaggcgagg gaacagcagc aggccgctaa cgaataaccc ctcactatag
     7441 gggaagggga cttaactatc actataggga gagaccctaa gtcaccagcc tgctggcatt
     7501 ctccgacaca catatacatt cattaagtct caggcttccg gtagcggacc gattggaaac
     7561 catccgcaga cgcctagtgt taaatcacct ggcctgagac cattctcttt aacgtaggtt
     7621 atcttccctt ttgacaggcg gggagcagaa gaccgcatcc gactttaagt tggctctgcg
     7681 ataatcgctg cgcggataac tgaagcacca agacatcatt gctgaggtct gccatccatt
     7741 tgtggacagg cggaaactca aagtggttga ccagtggagt ctgaggttgg actttgagtc
     7801 ctgaccagca ggctggagga gaccatgagt ttatcttgtt atggattgtg ttatcacaat
     7861 tccaataaga agaactttaa gtccagcctc cagtcctgtc agcccgaagt aaactatggt
     7921 cgctatgata tggtctccat
//
LOCUS       DAZSNO010000174         7899 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZSNO010000174 DAZSNO010000000
VERSION     DAZSNO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.176"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..718
                     /locus_tag="ACHWN2_003545"
     CDS             <1..718
                     /locus_tag="ACHWN2_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR8932928.1"
                     /translation="IMEKILFDNCILDDSNFAQIKMADGTLNACSAMHVQFYNAAMNR
                     ANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNL
                     NKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKA
                     CMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQT
                     EDNINAVAMKITADIMQHAD"
     gene            complement(872..2521)
                     /locus_tag="ACHWN2_003546"
     CDS             complement(872..2521)
                     /locus_tag="ACHWN2_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR8932929.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2672..4021)
                     /gene="ghxP"
                     /locus_tag="ACHWN2_003547"
                     /gene_synonym="yjcD"
     CDS             complement(2672..4021)
                     /gene="ghxP"
                     /locus_tag="ACHWN2_003547"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR8932930.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4727..5191)
                     /gene="soxR"
                     /locus_tag="ACHWN2_003548"
     CDS             complement(4727..5191)
                     /gene="soxR"
                     /locus_tag="ACHWN2_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR8932931.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5277..5600
                     /gene="soxS"
                     /locus_tag="ACHWN2_003549"
     CDS             5277..5600
                     /gene="soxS"
                     /locus_tag="ACHWN2_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR8932932.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5603..7180)
                     /gene="pdeC"
                     /locus_tag="ACHWN2_003550"
                     /pseudo
     CDS             complement(5603..7180)
                     /gene="pdeC"
                     /locus_tag="ACHWN2_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            7609..7890
                     /locus_tag="ACHWN2_003551"
     CDS             7609..7890
                     /locus_tag="ACHWN2_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFR8932933.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         2179 a         2050 c         1816 g         1854 t
ORIGIN      
        1 cataatggaa aaaatacttt ttgataattg tattctcgat gactcaaatt tcgctcagat
       61 aaaaatggcc gacggaactc taaatgcatg ctccgctatg catgttcaat tctacaatgc
      121 agcaatgaat agagccaata ttaaaaatac cttccttgac tattcaaatt tttatatggc
      181 gtacatggct gaggtaaatc tttataaagt aatagcgcca tatgttaatt tatttaaagc
      241 cgaccttagt ttctctaaac tcgatttaat taactttgaa catgctgatc tgtctcgcgt
      301 caatctgaac aaagcaatcc tccagaatat aaacttaatt gatagcaaac tcttttgtac
      361 gtggctgaca aatacattcc tcgaaatggt tatatgtacc ggctctaata tggctaatgt
      421 taattttaat aatgccaatt taagcaactg ccatttcaac tgttctattt taacaaaagc
      481 ctgtatgttt aatacccgtc tctatcgggt taatttcgat gaggctagcg tccagggaat
      541 gggcatttcc attctccgtg gggaggaaaa tatccccatt gatagtgata ccctggtaac
      601 actacagaaa ttctttgaag aagattgtac ctctcatact ggcatgtcac aaactgagga
      661 taatattaat gcagtcgcta tgaagattac tgcagatatt atgcaacacg cagattgagg
      721 ttaagtcgaa gaacagccaa tgaattgact ttacaagaga gataacttat ttgttttaga
      781 atcatgaatg ttcgcccgag ctaacctgtt aaaggtgcaa aagattgcac ctttttcaga
      841 ttacctcggg cctggttacg tcgctttcgg cttactgatt ttcctcaatt agcaacgctt
      901 caagcaaatc gagatcgtgc agtaattttt gcagcgtttc gttgctgatc tcccgcgtgg
      961 cgcgcaggtg gtacagttca gcacgttcag aacgcaatgc cgccagacgg aagcgacgct
     1021 caaggttctc ttcctgcacg gaactttcaa cgtcgttacg tccatcggcg cgacgacgca
     1081 ggttaccaat gacgcgagaa ctgacctccg taagcaactg gttatcgata ttctcttcgg
     1141 tatccgccgc cagacgctcc tccattttct ggatcgccac aatcgccact tctgccgttg
     1201 ccgctcgcgc aatacgttct tctttcaatt gttgcgaatg gtccgcgact tcaatgtgtt
     1261 gtagcagaat aggcaacatc accacgccga caaacagcga gaagagaatg acgccagccg
     1321 ccaggaacac cagctcatag cgcgccggga agacgttacc atccggcaag agcagcggga
     1381 ttgagagcac accggccaga gtgatcgccc cacgcacccc agcgaaagac gcgatcagga
     1441 tttctcgtgt ggtccacgaa ccaaactcca tcggcttctt cttcaggaag cggttgctga
     1501 actttttcat cgtccacagc cagccgaaac ggaccagcat cagcgccgca tatatcagaa
     1561 taatatcggt aaacagcatc cagatttcga cgttagggtc gatttctgcc gccatcagcg
     1621 acgtctccag aatacccggc agttgcagac ctaacagcag gaacaccatg ccgttaaaga
     1681 caaattccag catcgcccag gtgctgtttg cgcgcaggcg cattgccagt ggcgcacggc
     1741 gcatcacacc ggagcgggtg atggtcatcc cggcggcaac cgcagcgagg atgcccgaaa
     1801 cgccaatatg ttcggcaatc agataagaag cgaatggcag caagaacagc agaacgatct
     1861 gcgtcgcggg ttcatcaccg ccccagcggc tgaggaatcg cagcgagcga ccatacagcc
     1921 agctcaccac aaaaccggca agaataccgc caatggctac tttcataaat tcgaccgtcg
     1981 caccgccgac agtgaagatc atcgtcccca tcgccactgc cacggcaaac ttcaacgaca
     2041 ctaggccgga agcgtcattc atcaacgctt cgccctgcaa aatgcccatg atttttttcg
     2101 ggatgcgccc ttcgccaaca atcccggaga gcgccacagc atcggtcgga gaaagtaccg
     2161 ccgccagcgc aaaggcgggg atcagcggaa tgcccggcac cacccagtaa ataaggaagc
     2221 caatgccgac cacggtgacc accaccagcg ccagcgcgag gccgaaaatc tctcgaccat
     2281 gttcgagaaa ttcacgggtc ggcgttttcc agccatcagc gaacagcaac ggcgggataa
     2341 acaagactaa aaagagttca ggatcaaact ccacatgcaa accaaacgtc ggccacgcca
     2401 gtagcgcacc gatggcgatt tgcataagcg gaagcgggat ctgaaagggc atgacacgag
     2461 tgaccacccc ggacagcgag accacgaggg tcattatcag tatggtgaag aagatttcca
     2521 tgcgttccct gtttgcgatg ttttttatga tctgagaaag acgttatgcc tgaattctca
     2581 ttttttcaga atatcgcaaa agctcacaca atgtgcggag aaaataaaaa cgggcagcca
     2641 ttgccacccg ttttcagaat ccgatgaaag attagatagc ccagccaccc gcatagaagg
     2701 tcaccagcgc cacggcgata acgaccgtac cgatgttcaa cttgcgccat tcaccggaaa
     2761 ccagacgacc aatcaccaga gtcgcgaagc cgatcatgat gcctgttacg atgttacagg
     2821 tcagcacgat gaataccgcc gtaaccagac ccgccatcgc atcaacaaaa tcagcaaagt
     2881 cgattttcgc cacgttgctc agcatcagca ggccaacgta catcagcgcc ggagccgttg
     2941 cataccccgg aacgaggtaa gaaagcggag agaggaacag aatcagcagg aacagcacgc
     3001 caacggtgat agccgtcaac ccggttttac cgcccgccgc cgtacccgcc gcagactcga
     3061 tgtataccgc tgccggagct gcacccacca ggccagagaa aacgctgctc atggagtcag
     3121 tggtcagtgc tttcccgccg tcgatgatct gcccatcttt atccagcagg ttcgcctggc
     3181 cggcgacagc gcggatagtt ccggtggcat caaatactgc cgtcatcacc agcgccagaa
     3241 cgcttggcag gactacagga ttcagcgcgc ccataatgtc caggctgcca atcagtgaat
     3301 tgccgttttc atcgctcaat gaaggcatgg cgaaaacgcc ggagaaatgg acgttaggat
     3361 cgaagatcaa accgacaatt gagataccga taatggtcag cagaatgcca ccagggactt
     3421 tcagtttttc caagccgatg atcaccgcca gacctgccag tgacataatc accgggaagg
     3481 tcgcgaaatc acccagcgca acgggcagac catcaagcgg gtttttaatc accagaccga
     3541 caccattagc ggcaatgagc agcaggaaca gaccgatacc gatccccgtg ccgtgcgcca
     3601 caccgtgcgg caagttgcgc aaaatccagc tacggatacc cgtggcagaa attaccgtaa
     3661 acagcacacc catcaggaac acggcaccca gcgcgacagg tacgctaata tgttgcccca
     3721 gcaccaggct gaatgcggta aacgccgtca gggagatggc gcaaccaatc gccaacggca
     3781 gattagccca cagacccatc acgatagaac cgagtccggc aaccagacag gttgcaacga
     3841 aaactgccgc aggcgggaag cctgctttac ccaacatacc tggaacgacg atgaccgagt
     3901 agaccatcgc cagaaacgtt gttaacccgg caaccacttc ctgacggaca gtgcttccac
     3961 gttgtgaaat tttaaaccag gcgtcgagtg aaccgccggt acgcgctgat ggcgtagaca
     4021 taagaaaaca tcccctgaga gtttaatttt cgtcaagatg cgtggcggac aatcccctgc
     4081 cagcgaaacg ttatcttcct gctccaggtt tgcggcaatt tggtgcgtcg caaaaaagca
     4141 aacgtttagc tccgcgcata caaaacgggt ggtaaaaaaa agcaaacgat tatccggcga
     4201 tcatagcggc tttttcaacc gaagtttgct gcttttttct cttttattgt cttgacgatg
     4261 aaaaaccagg caattgatgc gcaaacgtta tcgtcgatgt gcaagattgc agcgttatcg
     4321 agccgtatac cgttctggat tgcgataaaa aatcgtctga gatgacaaaa atattgcggc
     4381 taaaaagtat acttttacac tggcgaagaa acgtcggtga catacaatta aagcatcaac
     4441 accaaccgga acctccacca cgtgctcgaa tgaggtgtgt tgacgtcggg ggaaaccctc
     4501 ctgtgttcca gcgggataga gagaaagaca aagaccggaa aacaaactaa agcgcccttg
     4561 tggcgcttta gttttgtgtt tgcggataat tccaaaagag ctcgatcagt cccctcgccc
     4621 cttcggggag agggttaggg tgaggggaac aggcagcacc agtgcaaata tttaccctca
     4681 ccccggcccc atagttaacg atgagtcagc ttgtccttgt ggcgctttag ttttgttcat
     4741 cttccagcaa gcgtgcgccg gtaccttctt ctcctaagcg gtcgcccggg ttacgcaacg
     4801 ggcaatcact gcgcgaaagg cagccacaac caatacatcc gtccagttcg tcacgcagcg
     4861 ccactaaggt atgaatgcgc cgatccaact cttctcgcca ttgggacgaa agctgtttcc
     4921 actctttcgc acttaacgta tgcccttctg gcaacacgcc aaacgcttca ccaatggtcg
     4981 ccagcggaat gccaatacgc tgagcgattt tgataattgc gacatatcgc aacacatcac
     5041 gtttatatcg ccgctgattg ccgctgttac ggatactagt aatcaaccct ttgctttcat
     5101 agaaatgcag cgccgatacc gccacaccgc tgcgtttcgc cacttcgcca ggggttagca
     5161 gcgctttaat gcggggtaat ttcttttcca taaatcgctt tacctcaagt taacttgagg
     5221 aattatactc cccaacagat gaattaacga actgaacact gaaaagaggc agatttatgt
     5281 cccatcagaa aattattcag gatcttatcg catggattga cgtgcatatt gaccagccgc
     5341 ttaacattga tgtcgtcgca aaaaaatcag gctattcaaa gtggtacttg caacggatgt
     5401 tccgcacggt gacgcatcag acgcttggcg attacattcg ccaacgccgc ctgttactgg
     5461 ccgccgttga gttgcgcacc accgagcgtc cgatttttga tatcgcaatg gacctgggtt
     5521 atgtctcgca gcagaccttc tcccgcgttt tccgtcggca gtttgatcgc actcccagcg
     5581 attatcgcca ccgcctgtag ttttattgcc cgcgcgttaa ctcccgcgcg ggtaattgct
     5641 ccatccattg cataaacacc tgcggcggca tcgccttcgc aaaaaaccat ccctggcaat
     5701 agcgcacgcc gcgcttgcgc agccagttaa cctgctcctc agtttcgacg ccttcagcga
     5761 tcgtttttaa ccccaggctg tgcgccagct cgatgatgtg ttccgcaatc aaatgactgg
     5821 ttttgtgggt ggtcagcgtt tcaacaaacg atttatcgat tttcaaaata tcgacattca
     5881 atgatttaag gttatgcaag ttagagtagc caataccaaa atcatcaatt gccacttcgt
     5941 aaccagcctg gcggaaagcc agaattaatc ggcgtcattt tttcgacatc aagaaatgca
     6001 tgctcagtca cttcaaattt aatttgctgc ggacgcaccg cgtattgctc tgttttctga
     6061 ttgattcgcg ctatcaaccg tgacgtatga aaatcggagg ccgacaggtt aatagaaaca
     6121 tagcgatcgg catgtgttgc caggtacgcg cccagatcgc ggaagacatt atcaataaca
     6181 taatcagtta tctgttctat catcccctcc ttttctgcca gcggaataaa ctctgccgga
     6241 ttcattattt gcccctgctc accaggccaa cgtaacaacg cttcagcgcc gatacatttt
     6301 tctgttttga tatcgattat tggctggtaa taaagacaaa gttgatgttt ttcgagggcg
     6361 cgttgcaatt tacgtttggg agataaatag ttttgtcgaa tacgtagcca gagtaatagc
     6421 agaacaagac tccccaaaat accggcgggc aacgcaaaaa taagatgatt ataaaaatgg
     6481 gttataagac gttgatatga agtcgcaaca atggctgcaa ttgggcgttt tgtcgaataa
     6541 attgtcgcat ataaatagcc atttctttgt acggttaaat ccttcaaatg aatcagcgga
     6601 gaaaacgttg ctgccgaggc ctctttgctt aacgagaaaa aggttttcat caccgtatca
     6661 tacacacccc attgcaatgt cgggtcatca gacatcactt cactccagaa gagagggttg
     6721 ataaccgcca cataatttcc ccgctgcata taggtcattt tatagccaga gaaaaaaggc
     6781 gtatcgcggt aataatagat agaaacgtta ggttcacgct tataatcggc cggtgcaatc
     6841 gtatagccgt ttacaggcgc tatcagcgat gagcataaaa aatggttatc acgaattcat
     6901 taatataaag atagccacga ataatattca acattcgctt ttgatgggct ggagtgcaaa
     6961 cttgcccctg atagcgttca gctgcatcgc tcactaaatc tgcctggcga atgaccagct
     7021 cggatttatc cagtgccagt tgagcaaagg ttcggagctg attattcact tctgattttg
     7081 cccaaaggaa ggcaatccat agcgaaagaa tgatgggaaa gagaactaaa aagataatgc
     7141 ccggcaacgc cagtaattgg tgtcgtgcac gatgactcat tgctatcaat ttcctgcctg
     7201 tttatacccg gccattgcaa ggtgaaaccc tggctaccga cgtctttaga taaatcttac
     7261 ggtataaata gcacttttgc ctgatttgaa attgatacct ttttaatcgt cacctggatg
     7321 aaaataattc attgatattc aaaattaatt ttctacagga atacagaggc ggcgggaaga
     7381 tatgccctta atgccactga aaaaccacat aatttgtaca gcaataaaag tcacggccta
     7441 atatggctaa caaacaatca attgtgatat agttcacaaa attaatgaaa caaacagagt
     7501 gtttcatttt tgtgttctgc aagccccgcg atagccagag tgtaccacgc ctcccgtgaa
     7561 caacgccgcg ctgtccaggg catcggctct tttcagagga attgtttgat ggctaccctc
     7621 accactggcg tggttcttct tcgctggcaa cttcttagtg ccgtaatgat gtttctggcc
     7681 agcacactca acatccgttt tcgtcggtct gattatgtcg ggcttgcggt gatcagcagc
     7741 ggtctgggcg ttgtttccgc ctgttggttc gcaatggggt tgcttggcat cacaatggcg
     7801 gacatcaccg ctatctggca caacatcgag tcggtgatga tagaagagat gaatcagaca
     7861 ccgccacaat ggccaatgat tttgacctga tacgtagaa
//
LOCUS       DAZSNO010000175         7841 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZSNO010000175 DAZSNO010000000
VERSION     DAZSNO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.177"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..183
                     /locus_tag="ACHWN2_003552"
     CDS             <1..183
                     /locus_tag="ACHWN2_003552"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932934.1"
                     /translation="FDLVNRDICWGGAICSLKRETRLSARNTEIKKPGHFFTKWPGKN
                     NFYFLTVLSLIEMSQN"
     gene            complement(188..263)
                     /locus_tag="ACHWN2_003553"
     tRNA            complement(188..263)
                     /locus_tag="ACHWN2_003553"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(228..230),aa:Asn,seq:gtt)
     gene            complement(364..1835)
                     /gene="yeeO"
                     /locus_tag="ACHWN2_003554"
                     /pseudo
     CDS             complement(364..1835)
                     /gene="yeeO"
                     /locus_tag="ACHWN2_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1996..2071
                     /locus_tag="ACHWN2_003555"
     tRNA            1996..2071
                     /locus_tag="ACHWN2_003555"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2029..2031,aa:Asn,seq:gtt)
     gene            complement(2109..3059)
                     /gene="cbl"
                     /locus_tag="ACHWN2_003556"
     CDS             complement(2109..3059)
                     /gene="cbl"
                     /locus_tag="ACHWN2_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR8932935.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3161..4078)
                     /gene="nac"
                     /locus_tag="ACHWN2_003557"
     CDS             complement(3161..4078)
                     /gene="nac"
                     /locus_tag="ACHWN2_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR8932936.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4406..4481
                     /locus_tag="ACHWN2_003558"
     tRNA            4406..4481
                     /locus_tag="ACHWN2_003558"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4439..4441,aa:Asn,seq:gtt)
     gene            complement(4537..5472)
                     /gene="ldtA"
                     /locus_tag="ACHWN2_003559"
     CDS             complement(4537..5472)
                     /gene="ldtA"
                     /locus_tag="ACHWN2_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR8932937.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5534..6613)
                     /gene="cobT"
                     /locus_tag="ACHWN2_003560"
     CDS             complement(5534..6613)
                     /gene="cobT"
                     /locus_tag="ACHWN2_003560"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR8932938.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6625..7368)
                     /gene="cobS"
                     /locus_tag="ACHWN2_003561"
     CDS             complement(6625..7368)
                     /gene="cobS"
                     /locus_tag="ACHWN2_003561"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR8932939.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7365..>7841)
                     /gene="cobU"
                     /locus_tag="ACHWN2_003562"
     CDS             complement(7365..>7841)
                     /gene="cobU"
                     /locus_tag="ACHWN2_003562"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR8932940.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2170 a         2106 c         1700 g         1865 t
ORIGIN      
        1 tttgaccttg tgaatcgtga tatctgttgg ggaggggcaa tttgctcact gaagcgtgag
       61 actcgattaa gcgcacgaaa cacagaaatc aaaaaacccg gccacttttt tacaaagtgg
      121 ccgggtaaaa ataattttta ttttttaact gtcctgagtc tcatagagat gtctcaaaac
      181 taaaatttgg ctcctctgac tggactcgaa ccagtgacat acggattaac agtccgccgt
      241 tctaccgact gaactacaga ggaatcgtga gaacgaggcg aatattagcg atgcccaccc
      301 acaatgtcaa agcctgtttt ttaaatttga aatcgtttgc tgaaataatc tgcattttgt
      361 cgtttattcc gacacaactg gctttttttc acacttttgc ggctcggatc gagggtattt
      421 ccacagccaa cgtccagtaa ccattcgcca gtaaaacagc acggcccgca cagcccagtc
      481 ggcaaacatt cccatccaga caccaaccac accccagcca agcatgattc cctgcacata
      541 accgactaca acccggcaac cccacatgct caacatagaa acccacatgg cgtaacgggc
      601 atcacgagca cccttaaatc cagcgggtag cacccatgag gcggaccaaa taggcataaa
      661 taaagcattt agctaaatca gaatcacaac gacgtgttta acctgtggat cctgggtgta
      721 aaacgatgcc ataaccccgg caaagggagc cgttagccag gcgatggccg ttaatccaag
      781 agtggacagc cagaacacat gccgcaactg aatctctgct tgcgctatct gccctacccc
      841 caaccttcgg cctgtaatga tcgtagaagc agaaccgagc gcacttccgg gtaagttgat
      901 aagagccgca attgagaacg cgataaaatt tccggcaata acgctggtcc ccatcccggc
      961 aacgaacatt tgggttaata accgaccact ggtaaataac actgattcga cactcgctgg
     1021 aataccaatc cccatgactt cccagataat gctaaaattc agcggtttaa aatagctctt
     1081 taacgaaatc cttagcgcag gattaaaacc aatcgccagc acccacaaaa ttgcaactgc
     1141 gccaatataa cgagaaatgg ttaaacccaa cccagccccg accaggagaa aaggccgtaa
     1201 atcaatatgc cgctaataat aatattaaga atattcaggc taccgttaat cagtagcggt
     1261 attttcgtat tccctgcacc acgaagtgcc ccgctaccaa taagagtgat ggcagcggct
     1321 ggataactga gtacggtcag ctccagataa gtcaacgcca gtgctttaac ttctgtcgtg
     1381 gcatcacccg cgacgaaatc aataatttgt tcgccaaaat gatgaattag cgttgccaac
     1441 agtacggcaa acaacgtcat gatcaccaat gactgccgcg tcgccaccct cgctcgtcgt
     1501 cgatcccgct taccgagact aaatgctaca acgacagtag taccaagatc gatagctgca
     1561 aaaaaagcca taatgaccat attgaagctg tccgccaacc ccacgccggc catcgcatct
     1621 tttcccagcc aactgaccag aaaagtgctc aggaccccca tcaacaggac acaggcgttc
     1681 tccatgaaga taggaacagc aagcggggtt atctcgcgct agaacaacac tttgtagctc
     1741 ttgcgtttag agtgccagcg agtgccgtga acaacctggc gtaaagcaga ggagatattc
     1801 aaagccgacc ttaattgcag aaagtgaaac cacatttcaa atgatgaggg agaatcagca
     1861 aagctgcaaa gattttcgcc aacaaattgt ctgcaaatgc aacaaactgt tgatagaaac
     1921 ggcaaacagt tgggaaattt aaaaatcggg tttgacaaaa tatttttcgc cgttaagatg
     1981 tgcctcaaca gcgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat
     2041 gtcactggtt cgagtccagt cagaggagcc aaatttctga aaagcccgct tttatagcgg
     2101 gctttttgct atatctgata atcaatttcc tcttcactgt tttccatcac ctggcgcttg
     2161 atatcctcaa ctgacagtcc tgcattacaa agttccagaa agcgccaaac atagttacgc
     2221 tgaagttgtc ctcgcttcag tcccaaccag acagtattag catcgaaaag atgccgcgta
     2281 tccaggcgga ttaaattctc ttcctcttgt tcgccgctgg attgttcggc aactaatccg
     2341 atcccaagcc caagagcaac ataggtttta atgacatcag aatcctgtgc acttaataca
     2401 atatctgccg gcaaaccttt gcgggtaaat gcgtcatcag tgcgtgagcg ccccgtaatt
     2461 ccctgtcggt aagtaattaa cggccacttc gctattgatt ccagcgtcaa tggtgtaatt
     2521 tgcgttaagg gatgatcgag tggaacaagc aaactatggt gccaacaaaa ccatgggaag
     2581 gcgacgagct gcgggtcatt actcaaacgc tcgctggcga taccaatatc agcttcgcca
     2641 ttttgcaaca acgtcgcaat ttcctgtggc gaaccctgga ttagctcgag ccgaacctcc
     2701 gggaaaagtt cgcgaaaagc tttaatgacc tctggcaagc tataacgtgc ctgagtatgc
     2761 gtcgttgcaa tagtgagaac accagacgta tcgttggtaa acaggtctgc aagccgacga
     2821 acattactgg cttcattcag aatacgttct gcaatgacca gtaatgcttt gcccggttca
     2881 gtcatcccca gcagtcgctt acctcgtcga acaaatattt cgatgccaaa ttcatcctcc
     2941 agttcccgaa tatgacggct aacgcctgac tgtgaggtaa aaagcatatt cgcaacctct
     3001 gtcaggttgt aatcctgacg tgcagcctcg cggattatct ttagttgttg gaaattcacg
     3061 gtaaactccg ggcagttcag atatcccgtt attgttaaag tctaatgccc ggcataacaa
     3121 ataataaaaa cccgcatctt attccatccc gatataacgc ttagctcacc aattgccact
     3181 gccttttttc catcactgga gaactaatca ctgacattaa caactctttc actgcctgtg
     3241 cctgtggtga caagtttgct ctggcgggta aatttaagga caaagagaga ctcattgaag
     3301 gagtggtaat gcgtgacatc cacccattta ctgcgccaca taacgaacgc gcagccgatt
     3361 caggtaatac tgcaacgccc atgccgctgg caatcgctgc ggtaagcgtg gcaatagact
     3421 caatttcacc aatgactttt gccgtgagcc gccgtaggga aaaagcctca tcaacacgaa
     3481 gtctaacagc gctgtaatca ctggggagaa aaaggttcat ttgcgcaata gcattaacat
     3541 caacgttttg ccccgggcaa tcttgagttc ctaccaggaa aagatcttct ttcagcaaag
     3601 cctgactgga taccccagcc acaggggaat gctcataaat caccgccata tcgagttggt
     3661 gatttatcag tttttcgtta agcactgcac cactattttc atgaagatag ataacgacct
     3721 ccgggaattc agcgcgaacc gcctgtaata agggcatggt gatggatgac gcagcggttc
     3781 ctggcgcaaa gccaatcgag acttgccccg ataatgactg accaacgtta tgcaccgcca
     3841 gttgggcctg ctcacactga cgtaaaatgg cccgtgcatg ggtatagaga atttttccgg
     3901 cgtctgttgg cgtaacgccc cgttttgtgc gaatcaaaag ctgttgattt aactcacctt
     3961 ccagtatggc aacctgttgg ctgagcgctg gttgtgcgat atgcaatact tcagcagcct
     4021 gggtcaggct accaatatct acaattttta cgaagtattt caggcgtctg aagttcatgt
     4081 tgcctccggt ttttaagaat cggcccaagt gccgccatta cttacaacca gtatgcaaga
     4141 tgcctgccag ttctattttg gcgttgatgc gcaagctaac caactgtcaa ataagagatt
     4201 atgatagatt cgtcattggc cctttttatc agcagtcgca ttcacctgcc ctataaaagg
     4261 agggtatgca ccacgatggt tcattaccca ataagattga aagctcacca ctttgttgaa
     4321 attggcagca aacaaacaaa aaaaatgcat ttcacccttt gacatcacca tgcactgcca
     4381 ttaatatgcg ccccgttcac acgattcctc tgtagttcag tcggtagaac ggcggactgt
     4441 taatccgtat gtcactggtt cgagtccagt cagaggagcc aaattcaaaa aagcctgctt
     4501 tcgagcaggc tttttacttt ctaattacca acgctcttaa aacatctgtc ttgaaccaga
     4561 actaatttgc acaggcattc ccgatcgatg ttgcaacgca gcatttgcgc gatttacatc
     4621 aacttcttgc ccgttgataa acgcccgcaa agatggggtt accggcaatg gcacttttcg
     4681 gtcagactca tattctgcac gattgcgcga cagcggctca tgaacttcca gccactttga
     4741 accatccggt tccgttgtgt atttcactgg ctgatcaata atttgtacac gagtgcctac
     4801 aggaacatta tcaaacagat atttgatatc gtcattacgc agacgaatac agccctgact
     4861 tacacggagc ccaataccaa aattggcatt ggtaccgtgg atggcataca acctgccaat
     4921 ataaatcgcg tacagcccca tggggttatc gggccccgca ggaacaaatg cgggcaaact
     4981 ctcccctcgt ttcgcatatt cgcgccggat gttcggcgtt ggcgtccagg ttggtgcttc
     5041 ttgtttacgt tcaacggtag tcacccagtt acgcggggtt tctcgcccag cctggccgat
     5101 accaatagga aagacttcca ctgtattact gtctggcgga tagtaataaa gacgcatttc
     5161 agcgacgtta accacaatcc ctttacgaac agtggcgggc aaaatcagtt gctgcggaat
     5221 ggtgagttgt gagccaggct tcggcaaaaa aacatcagcg cccggattcg cttccagcat
     5281 attacttaac ccttgcccgt attgtgcggc aaaagtctcc agcggctgag tattgtgatc
     5341 aggaacagtt acagtaagcg actgccccac taaacggcta ccctctggag gtaatggata
     5401 agttaccgcc aggctatttt ggctggcaaa aagcagagca aatgagcaaa gaatatttac
     5461 acgacgcatc atgtcccttt cctaagtcgc gaatactatc cgataagtat agcttttatc
     5521 agacttttcg tttttagctg ttcaaatcag aagtcgtatt ccccggtaga acaatattac
     5581 tggcagcaag ttcgcccatg ttgttgtata tcgcacaagc agcttcgatg atggacatcg
     5641 ccagagctgc accactcccc tcacctaaac gcatatccat attgagataa ggctccagcc
     5701 ccaaatgcga gagcgctata cgcgcgcctt tttctgccga caagtgagaa ggaatgagat
     5761 acggtttgat tgcaggagac atctggcagg ctgcgagcgc agcagcataa gaaagaaatc
     5821 catccagcaa cacgggtaaa ccgcaggaag cagcacctaa catcactcca gctattccga
     5881 ccaaatcaaa tccacccact tttgccagga catcaacacc atcctgagga tttggttgat
     5941 tcaacgtaat cgcccgacgc acaacatcaa ttttattagc cagtttatct gtcggcaggt
     6001 ttgcgccaat cccaaccact tcttcaggat cccggccagt gattgtgctg actattgccg
     6061 ctgccggtgt cgtgtttgcc atccccagtt cacctacacc aaacagcgtg acaccgtttt
     6121 tcgccagctc ccgcgtataa catatgacgt ccaaaagcaa cttttcagcc tgacggcgac
     6181 tcattgccgg agctgaagca atattgccgc tacctcgtgc gacacgcatg ttgataagcc
     6241 cggggatagg ctcagcagta tcaataccaa catcaattac gtggacgtta gcgcccgctt
     6301 gtgctgccag cacacacacg ccggttgttc cacgggtcat attttcagcc tgtatggctg
     6361 tcacttcttt tggagaaata gcgacccctt cctcccagac gccgtgatcg gcacacataa
     6421 ccagtaccgc ttttttgccc acatgcggta tgccattcac ccccggcatt cctgccagtt
     6481 gtatggcaag cgcctccagc tttcctaggc taccaacagg tttgaataac ccgtcaacat
     6541 gccgttgtgc acgcgacata gcggcagaat tgatggctgg gatcgtattc agtaaatcgg
     6601 caattatttg catgtcacgt ttctttatag cagtgccagc aggaagacca gttcaccaag
     6661 ctctatggct gctcccagcg tatcgcccgt ttgcccgccc agcgtacgtt tgagaagctg
     6721 accgaggatg aaaatcgcca ccatcgtcac caccatagca gccacaccat gcatacccag
     6781 caataatacg gcagcaaaaa tagcggctaa gccgagtgtg acgcaggttt gtcgcccatc
     6841 aattttgcca atgaacacat tgccaagccc ttcttcccgc gcataacgat gacgatacat
     6901 caataatgcg gcagtcccgc ggctgaccgc acatgctgcc gccagcgatg caaggatcgg
     6961 ttcgccacgc agagccaact cgcttaacac cagaatcttt gccagtacca caaaaatcaa
     7021 tgccagaccg ccgtgggtgc ctaaacggct atcacgcatg atttccaaca tgcgatcgcg
     7081 actacgtgca gaaaatacgc catcacaggt atcagcgagg ccgtccagat ggaattcccc
     7141 ggtcatcagc gccagcacaa gtacgctaaa cagtgccgcc agtggtgcgc cacaccatgc
     7201 ctgcagcacc atgaagacca gcccgctaat cgcgccaagc aataatccaa tcaaaggaaa
     7261 agtaataata ccgcgagaat aatgttcgaa atccagtccc tgggaccagc gacgcggtac
     7321 aggcagacgc gtaataaatg agagcatcgc ccaaaataat ttgctcattt gatttttact
     7381 ccaatacccg aaaccaccag ccatacttca tttgccgcag cggccaactg ctgatttacc
     7441 cgcccggcaa tatcacgaaa atgtcgtgcc agacgactct ccggcacaat ccccattccc
     7501 acttcgttag tcactaatac aacctttgcg gggcaacgtt ggcaggcagc aatcaacgac
     7561 tgaatctcag cattaatcgc ctgttccatc gcctgataat cccattcatc agggtcttta
     7621 tcgccgccat aatcaaacaa cagattagtc accattgtgg taacgcattc cagcaacaca
     7681 gcctcattcg ggttaatgtc tgcatgaatt aattcatcaa gatgttgcca gcgctccact
     7741 gtgcgccagt gctccgggcg gctttgccga tgatgttcta tccgtgcagc catctcatca
     7801 tcaaggattt gcgaggtagc gatatacaga acctgtgaag a
//
LOCUS       DAZSNO010000176         7743 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZSNO010000176 DAZSNO010000000
VERSION     DAZSNO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.178"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="ACHWN2_003563"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="ACHWN2_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFR8932941.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="ACHWN2_003564"
     CDS             1102..2562
                     /locus_tag="ACHWN2_003564"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR8932942.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="ACHWN2_003565"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="ACHWN2_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR8932943.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="rspR"
                     /locus_tag="ACHWN2_003566"
                     /gene_synonym="ydfH"
     CDS             complement(2979..3665)
                     /gene="rspR"
                     /locus_tag="ACHWN2_003566"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR8932944.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="ACHWN2_003567"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="ACHWN2_003567"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR8932945.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="ACHWN2_003568"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="ACHWN2_003568"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR8932946.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="ACHWN2_003569"
     CDS             complement(6727..7245)
                     /locus_tag="ACHWN2_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR8932947.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7743
                     /gene="ydeI"
                     /locus_tag="ACHWN2_003570"
     CDS             7521..>7743
                     /gene="ydeI"
                     /locus_tag="ACHWN2_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR8932948.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1940 a         1812 c         1956 g         2035 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 aag
//
LOCUS       DAZSNO010000177         7576 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZSNO010000177 DAZSNO010000000
VERSION     DAZSNO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7576
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.179"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(340..1740)
                     /gene="asnS"
                     /locus_tag="ACHWN2_003571"
     CDS             complement(340..1740)
                     /gene="asnS"
                     /locus_tag="ACHWN2_003571"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR8932949.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1909..3111)
                     /gene="pncB"
                     /locus_tag="ACHWN2_003572"
     CDS             complement(1909..3111)
                     /gene="pncB"
                     /locus_tag="ACHWN2_003572"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR8932950.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3377..5992
                     /gene="pepN"
                     /locus_tag="ACHWN2_003573"
     CDS             3377..5992
                     /gene="pepN"
                     /locus_tag="ACHWN2_003573"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR8932951.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(6199..6966)
                     /gene="ssuB"
                     /locus_tag="ACHWN2_003574"
     CDS             complement(6199..6966)
                     /gene="ssuB"
                     /locus_tag="ACHWN2_003574"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR8932952.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(6963..>7576)
                     /gene="ssuC"
                     /locus_tag="ACHWN2_003575"
     CDS             complement(6963..>7576)
                     /gene="ssuC"
                     /locus_tag="ACHWN2_003575"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR8932953.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         1930 a         2004 c         1910 g         1732 t
ORIGIN      
        1 ttacagcaat ttttatctat ttatatgatt tacttatatt taaattaact aaacggaaat
       61 tttgtttctg atggaaactt tatcgacctg gcacaaaatc ctctttcaga catccagaat
      121 gccaaaaaat agtatgaaaa ttgtgctatc agctaagaaa aaactaatcc gcaataagag
      181 tacgggttat tttttgtgcg taaatgttgc aatgtaatat tccgtaacag gatgatcgtt
      241 atggcggggg tgcaaataga ttgcttgact taacaaacag ataacttgac agaaaggata
      301 aaaaagagcc agcaggcgct ggctctttga ggaagaaaat cagaagctgg cgttgcgcgg
      361 agtacgtggg aacggaatca catcacgcac gttttgcacg ccagttacgt aagcaatcag
      421 gcgttcaaaa ccaagaccga aacctgaatg cggaacagta ccgtagcgac gcagatcgcg
      481 ataccaccag taatcttctt tattcaggcc catttccagc atacgctcgt ccagaacgtc
      541 cagacgttct tcacgctggg agccaccaat gatctcaccg atgcccggag ccagaacgtc
      601 catagccgca acggttttac cgtcttcgtt gaggcgcata tagaacgctt taatatcttt
      661 cggatagttt ttaaccacta ccggtgcttt aaagtgttct tccgccagat aacgctcatg
      721 ctcagaagag agatcgacgc cccagtaaac cgggttttca aacttcctgc cgcagttttc
      781 gagaatggtc actgcgtcgg tgtaatccac ctgcgcgaaa tcggcttcaa tgaagcgttc
      841 cagacgtgaa acggcatctt tatctacgcg ttcagcgaag aatttcatgt cgtctgcgcg
      901 ttcttcgaga accgctttga agacatattt cagcatggct tcagccagac ccgcgatatc
      961 gttcaggtta gcaaacgcca cttccggctc cagcatccag aattccgcca ggtgacggct
     1021 ggtgttggag ttttcagcac ggaaagtcgg gccgaaggta taaattttgg ataatgcgca
     1081 agcgtaggtt tcgccgttca actggccaga tacggtcagg aaagactctt taccaaagaa
     1141 gtctttgtcg aaatccactt tgccctgatc gttacgcggc aggttttcca gatccagcgt
     1201 agaaacgcgg aacatttcgc ctgcaccttc ggtatcagat gcggtaatca gtggcgttga
     1261 aacccagaag aatccctgct cgttaaagaa gcgatgcagc gcctgcgcca gcgtatggcg
     1321 aacgcgcgcg acggcaccaa tcaggtttgt gcgcggacgc aggtgagcga cttcacgcag
     1381 atactcaatg ctgtggcgtt ttgccgccat cgggtaagtg tctggatctt caacccaacc
     1441 agcaacttca accttgctgg cctgaatttc aaattgttgc ccctggcccg gcgatgccac
     1501 gactttaccc gtcacaatga ccgagcagcc ggtggtcaga cgcaggacgt cttcattgta
     1561 attgggcaga gaattattga tgacagcctg tacaggatca aagcaggaac cgtcataaac
     1621 ggcgaggaag gagatgccag cttttgaatc tcggcgggta cgtacccatc cgcgtacggt
     1681 gacttcgctg tcaacggcta cgcggccctg gagtacgtcg gctacaggca caacgctcat
     1741 aatattctct ctgttaatag tcggaaaaaa taaacacttg tccacccgta aatggggggg
     1801 atacctatgt tacctggcat ctgcaatcag acaagcagaa ttcgcaaatg cagcgaaaga
     1861 tttcggaatt aaaggtaaaa aagggagccg attagctccc tgatgatatt aactggcttt
     1921 tttaatatgc ggaaggtcga acgctttgcg cagcgcccga acaaacgctt tatcatggca
     1981 gatagttttg ccagggctgt cagaaagttt cgccaccggt ttaccgttac actctaccaa
     2041 cttaatcaca atattcaggg gttttacctg ggggatatcg caggtcaggc gagtcccaat
     2101 accaaaactt aattgcacgc gggaagagaa gtggcggtat agctcaaccg ctttgcgtaa
     2161 atccagattg tcagagaaaa ccagcgtttt actctgtgga tcaattccca gcttttcata
     2221 atgtgcaatg gctttttcac cccattcaac cgggtcgcca gagtcatgac gcaggccctg
     2281 ataccgacta gcgaactcga caccgaaatc acgcaggaaa gcatccatag tgatgcagtc
     2341 ggttaatgca atgccaagtt ggtcgggata ctcttccagc caggcagcaa gtgcagctcg
     2401 ctggctgttg gctagatccg ggctgatttg ctgatgtgcc tggaaccatt cgtgtgcctg
     2461 tgttcccatc ggcgtgaggg aaagccgacg cgccagatcg tagttgctgg tgcccacgaa
     2521 ccaggattcc tgttgcagac gcttaacgat ggtttcttgt acttcgcgag aaaaacggcg
     2581 acgggtgcca aaatccatca gatggaagcg cgacatatca agaccggcgg ttaacgccga
     2641 gaagtcgact aatttgcttt ccagcgtgtc gagggcttgc gcaacgtcgg cctgcggtga
     2701 gcgatagcga tgtaccattt cactgataac cgccagcaaa ggaacttccc agaggatgac
     2761 ttcacgccac ggaccgctta agcgaatatc cagcttgcca ttatcgttgg acacggtgac
     2821 ttgttccggg ttaaagcgga actcgcgtaa ccagttaaga tagtcggcct taaagaaagg
     2881 cagggcagag agccactgat attcatcatc ctgcaggcgc aggtgctgca tcgcctgaat
     2941 ctgttcacga atagcatcgg cataaatacc cagcagatcg tcacctcggc aacgaaactc
     3001 cgccgcgaca tgcacatcgt aatagtgatg aaacacggct tgctgcatat gcaacttata
     3061 agcatctgta tccagcaacg agtgcagaac aggagaagcg aattgtgtca taggtgcgca
     3121 gtagcgtcct cgtacaggag cgtttagtac aataaacatc ttcaggaact gctggagtat
     3181 accttgtttc gcaatttatt gaaccccgat cacaccatat gccacctttc tggtcgatgg
     3241 cattacgcgg tgtatgttat aaaaatgtag caataaaggc gtttgtacct gaaaagatga
     3301 agattctgca tagcgcgatt tacgcaacag gaatagactg aacaccagac tctataaaag
     3361 atgctaaagg ttatttatga ctcaacagcc acaagccaaa taccgtcacg attatcgtgc
     3421 gccggattac cagattactg atattgactt gacctttgac ctcgacgcgc aaaagacggt
     3481 cgttaccgcg gtcagccagg ctgtccgtca tggtgcatca gatgctccgc ttcgtctcaa
     3541 cggcgaagac ctcaaactgg tttctgttca tattaatgat gagccgtgga ccgcctggaa
     3601 agaagaagaa gagggcgcac tggtcatcag taatttgccg gagcgtttta cgcttaagat
     3661 cattaatgaa ataagcctgg cggcgaatac ggcgctggaa gggctttatc agtcaggcga
     3721 tgcgctttgc acccagtgtg aagccgaagg tttccgccat attacgtatt atctcgaccg
     3781 cccggacgtg ctggcgcgtt ttaccaccaa aattattgcc gataaaatca aatatccctt
     3841 cctgctttcc aatggtaacc gcgttgcgca aggcgaactc gaaaacggac gccattgggt
     3901 acagtggcag gacccgttcc cgaaaccgtg ctacctgttt gcgctggtgg caggcgactt
     3961 tgatgtactg cgcgatacct ttaccacgcg ttctggtcgc gaagtagcac tggagctgta
     4021 cgtcgatcgc ggcaaccttg atcgcgcgcc gtgggcgatg acctcgctga aaaactccat
     4081 gaaatgggat gaagaacgct ttggcctgga gtatgacctc gacatctata tgatcgtcgc
     4141 ggtggatttc ttcaatatgg gcgcaatgga gaataagggt ctgaatatct ttaactccaa
     4201 atatgtgctg gcccgcaccg acaccgccac cgacaaagat tacctcgata ttgaacgcgt
     4261 tatcggccat gaatatttcc ataactggac cggtaaccga gtgacctgcc gcgactggtt
     4321 ccagctcagc ctgaaagaag gtttaaccgt cttccgcgat caggaattca gctctgacct
     4381 tggttcccgc gcagttaacc gcatcaataa tgtgcgcacc atgcgcggat tgcagtttgc
     4441 ggaagacgcc agcccgatgg cgcacccaat ccgcccggac atggtcattg agatgaacaa
     4501 cttctacacc ctgaccgttt acgagaaggg cgcggaagtg atccgtatga tccacaccct
     4561 gctgggcgaa gaaaacttcc agaaagggat gcaactctat ttcgagcgtc atgatggtag
     4621 cgcagcgacc tgtgacgact ttgtgcaggc gatggaagat gcgtcgaatg ttgatctctc
     4681 ccatttccgc cgttggtaca gccagtccgg tacgccggtt gtgaccgtca aagacgacta
     4741 caatccggaa actgagcagt acaccctgac catcagccag cgcacgccag ccacgccgga
     4801 tcaggcagaa aaacagccgc tgcatattcc gtttgccatc gaattgtatg acaacgaagg
     4861 taaagtgatc ccgttgcaga aaggcggtca tccggtgaat tccgtgctga acgtcaccca
     4921 ggcggaacag acctttgttt ttgataatgt ctacttccag ccggtgcctg cgctgctgtg
     4981 cgaattctct gcgccagtga aactggaata taagtggagc gatcagcaac tgaccttcct
     5041 gatgcgtcat gcgcgtaatg gtttctcccg ttgggatgcg gcgcaaagtt tgctggcaac
     5101 ctacatcaag ctgaacgtcg cccgtcatca gcaagggcag ccgctgtctc tgccggtaca
     5161 tgtggctgac gctttccgcg cggtgctgct cgatgagaag attgatccgg cgctggcggc
     5221 agaaatcctg acgctgcctt ctgtcaatga aatggctgaa ctgttcgata tcatagaccc
     5281 gattgctatt gccgaagtac gcgaagcact cactcgtact ctggcgactg aactggcgga
     5341 tgagctgctg gctatttaca acgcgaatta ccagagcgag taccgtgttg agcatgaaga
     5401 tattgcaaaa cgcactctgc gtaatgcctg cctgcgcttc cttgcttttg gtgaaacgca
     5461 tctggctgac gtgctggtga gcaagcaata ccatgaagca aacaatatga ccgatgcgct
     5521 ggcagcgctt tctgcggcgg ttgctgcaca gctgccttgc cgtgacgcgc tgatgcagga
     5581 gtacgacgat aagtggcatc aggacggtct ggtgatggat aaatggttta tcctgcaagc
     5641 caccagcccg gcggcgaatg tgctggagac ggtgcgtggt ctgttgcagc atcgctcatt
     5701 taccatgagc aacccgaacc gtattcgttc gttgattggt gcgtttgcgg gcagcaaccc
     5761 ggcagcgttc catgccgaag atggcagcgg ttaccagttc ctggtggaaa tgcttaccga
     5821 cctcaacagc cgtaacccgc aggtagcttc acgtctgatt gaaccgctga ttcgcctgaa
     5881 acgttatgat gccaaacgtc aggagaaaat gcgcgcggcg ctggaacagt tgaaagggct
     5941 ggaaaatctc tctggcgatc tttacgagaa gataactaaa gcactggctt gatacttttg
     6001 agccaaaatg cctgatgcga cgcttgacgc gtcttatcag gcctacaact ccgttgaaat
     6061 tgctggtttt gtaggctgga taaggcgttc acgccgcatc cggcagccgt gcccaactgc
     6121 ctgatgcgat gcttgacgtg tcttatcagg cctacaagtc cgttgaaaat gctggttttg
     6181 tgcgccacat ccggcatatt aaccatgacg ccgaatcagt tgttcagaat gcccacgccg
     6241 cattacccgt tgcaatactt ccgcttccag ctccgctaat cgcaccgacc ctaatcgacg
     6301 cggacggggg atatccactg tcaaatccag actaattttt ccctcttcaa ttaacaacac
     6361 ccggtcagcc attgctaccg cttcgctcac atcatgcgtc accaacagta cggtaaagcc
     6421 gtgctgctgc caaagtgaca caatcaaatc ctgcatctcg agtcgcgtta aggcgtccag
     6481 tgccccgagc ggttcatcaa gcaacaataa acccggtcga tgaatcaacg ctcttgccag
     6541 cgccacacgc tgtttctgcc cgccagaaag tgcggcaggc cattccccgg cgcgattctc
     6601 cagtcctgcc gcagccaacg cccgacgcgc ggcatcgcgc cactgacctt taaggcctaa
     6661 cccaacgtta tcaatcaccg atttccacgg cagcagacgc gcatcctgaa acatcattcg
     6721 cgtatcatcc tgaatttcag ccagcggtgt ggtgcccgct aacacatcgc ctgcggttgg
     6781 cgtttccaga ccggccagca ggtgcagcag ggtactttta ccaccaccgc tgcggcccac
     6841 caccgccaca aactgacctg ccggaatatg taaatccagt tggttcagga cgatattttc
     6901 cgcgtaatgt ttgcttactg cattgagcaa caatggtgtg ccctggttca gacgagcagt
     6961 attcatatcg tggcctcctt caaatgataa gccgggttcc agcgcaacca gaggcgctct
     7021 aacagctgcg cgctgacgtc agcaagtttg ccgagcaggg cgtaaagaat aatagcgact
     7081 accaccacgt ctgtttgcaa aaactctcgc gcattcatcg ccagataacc aatgccggaa
     7141 ttggcagaaa tggtttcggc aacaatcagc gtcagccaca tcaggcccaa cgcaaaacgc
     7201 acgccgacca taattgaggg cagggcatcg ggcaggatca catggataaa cagcggtatg
     7261 ccggataatc catagctacg cgccatctcc accagcccgc gatcgatatt acggatgcca
     7321 tgccaggtgt tgatataaat ggggaacagc gtaccgagcg ccaccagaaa gattttcgcg
     7381 gattcatcaa tgccaaacca caaaatcacc agcgggatca gcgccagatg cggcacgttg
     7441 cgcaacatct gaattgaggt atccagcagc ctttctcccc agcgcgacag tccgctaatc
     7501 agtcccagaa tcaggcccag cgatccgcca attgaaaagc caatcagcgc ccgccaggaa
     7561 ctgatcgcca gatgct
//
LOCUS       DAZSNO010000178         7538 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZSNO010000178 DAZSNO010000000
VERSION     DAZSNO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.180"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="ACHWN2_003576"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="ACHWN2_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="ACHWN2_003577"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="ACHWN2_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR8932954.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="ACHWN2_003578"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="ACHWN2_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="ACHWN2_003579"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="ACHWN2_003579"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR8932955.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="ACHWN2_003580"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="ACHWN2_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR8932956.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="ACHWN2_003581"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="ACHWN2_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR8932957.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="ACHWN2_003582"
     CDS             complement(6510..7202)
                     /locus_tag="ACHWN2_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR8932958.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1915 a         1830 c         1986 g         1807 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccgggat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagaaaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcaccccc ccccccgcgc cgctgagaaa aagcgaag
//
LOCUS       DAZSNO010000179         7386 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZSNO010000179 DAZSNO010000000
VERSION     DAZSNO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.181"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..162
                     /locus_tag="ACHWN2_003583"
     CDS             <1..162
                     /locus_tag="ACHWN2_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HFR8932959.1"
                     /translation="TEATDKKEEIIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKL
                     LLNNSHSNI"
     gene            175..564
                     /gene="ipgA"
                     /locus_tag="ACHWN2_003584"
     CDS             175..564
                     /gene="ipgA"
                     /locus_tag="ACHWN2_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFR8932960.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            580..1206
                     /gene="ipgB1"
                     /locus_tag="ACHWN2_003585"
     CDS             580..1206
                     /gene="ipgB1"
                     /locus_tag="ACHWN2_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFR8932961.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            1263..1730
                     /gene="ipgC"
                     /locus_tag="ACHWN2_003586"
     CDS             1263..1730
                     /gene="ipgC"
                     /locus_tag="ACHWN2_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR8932962.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            1736..3478
                     /gene="ipaB"
                     /locus_tag="ACHWN2_003587"
     CDS             1736..3478
                     /gene="ipaB"
                     /locus_tag="ACHWN2_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR8932963.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            3441..4589
                     /gene="ipaC"
                     /locus_tag="ACHWN2_003588"
     CDS             3441..4589
                     /gene="ipaC"
                     /locus_tag="ACHWN2_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR8932964.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKPTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            4640..5638
                     /gene="ipaD"
                     /locus_tag="ACHWN2_003589"
     CDS             4640..5638
                     /gene="ipaD"
                     /locus_tag="ACHWN2_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR8932965.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            5647..>7386
                     /gene="ipaA"
                     /locus_tag="ACHWN2_003590"
     CDS             5647..>7386
                     /gene="ipaA"
                     /locus_tag="ACHWN2_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR8932966.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKV"
BASE COUNT         2757 a         1463 c         1204 g         1962 t
ORIGIN      
        1 accgaagcaa cagataaaaa agaagaaatc atcgaaaaat caataaaaat tattgattat
       61 tacaattctc ttaaatcgcc agatcttgga accaaacttt acattcacga cttattacag
      121 atcaataagc ttttattgaa taactctcat tctaatatat agaaggccat agaaatgtgt
      181 cgcaaactat atgataaact ttatgaaata acaggtgcaa agcttgattt taacgataaa
      241 aatcaagcct ttatattgct cgaagaacaa atacctgttt gtataacaga taatgatgaa
      301 tacatctttt tgaccggact tcttaatgaa catgaacttt ttacagaaaa tattattaat
      361 ccagaacaca tactcatcct taactactct ttgagtagag attatggttc ttctatctgt
      421 cttctccctg atactcatca atgtgttttg accaagaaac actacaaaaa atatctttca
      481 cctgatgaac tcattgaatc tttgtatgaa tttttatttt gtattaaact aaccattgca
      541 aacatcactt cagaagtgaa ctaacatata gggggtatca tgcaaattct aaacaaaata
      601 cttccacagg ttgaattcgc gattcctcgc ccatcatttg attcgttaag tcgcaataag
      661 cttgtaaaga aaatattatc tgtttttaac ttaaaacaac gctttcctca gaagaatttt
      721 ggatgcccgg tgaatataaa taaaatcagg gacagcgtca tagacaaaat aaaagatagt
      781 aattcgggga atcaactatt ttgttggatg agccaagagc gaaccactta tgtctcttca
      841 atgataaaca gaagtataga tgaaatggcc atacacaatg gagttgtttt gacctcagat
      901 aacaaaaaaa acatatttgc agctattgaa aaaaaattcc cggatataaa acttgatgaa
      961 aaatcagcac aaacatctat cagtcataca gcactaaacg agattgcctc atctggcctc
     1021 agagcaaaga ttttaaaacg ctatagcagt gacatggatt tgtttaacac acaaatgaag
     1081 gatcttacaa atctagtatc cagttccgtc tacgataaaa tatttaatga atcaacaaaa
     1141 gttttacaaa tagaaataag cgcagaggtt ctaaaggctg tataccgtca aagcaataca
     1201 aattaaatct tttatattaa atcttatact tattaaagta aagcagacaa aaaggagacc
     1261 ttatgtcttt aaatatcacc gaaaatgaaa gcatctctac tgcagtaatt gatgcaatta
     1321 actctggcgc tacactgaaa gatattaatg caattcctga tgatatgatg gatgacattt
     1381 attcatatgc ttatgacttt tacaacaaag gaagaataga ggaagctgaa gttttcttca
     1441 ggtttttatg tatatacgac ttttacaatg tagactacat tatgggactc gcagctattt
     1501 atcagataaa agaacagttc caacaagcag cagaccttta tgctgtcgct tttgcattag
     1561 gaaaaaatga ctatacacca gtattccata ctggacaatg ccagcttcgg ttgaaagccc
     1621 ccttaaaagc taaagagtgc ttcgaactcg taattcaaca cagcaatgat gaaaaattaa
     1681 aaataaaagc acaatcatac ttggacgcaa ttcaggatat caaggagtaa ttattatgca
     1741 taatgtaagc accacaacca ctggttttcc tcttgccaaa atattggctt ccactgagct
     1801 tggagacaat actatccaag ctgcaaatga tgcagctaac aaattatttt ctcttacaat
     1861 tgctgatctt actgctaacc aaaatattaa tacaactaat gcacactcaa cttcaaatat
     1921 attaatccct gaacttaaag caccaaagtc attaaatgca agttcccaac taacgctttt
     1981 aattggaaac cttattcaaa tactcggtga aaaatcttta actgcattaa caaataaaat
     2041 tactgcttgg aagtcccagc aacaggcaag acagcaaaaa aacctagaat tctccgataa
     2101 aattaacact cttctatctg aaactgaagg actaaccaga gactatgaaa aacaaattaa
     2161 taaactaaaa aacgcagatt ctaaaataaa agacctagaa aataaaatta accaaattca
     2221 aacaagatta tccgaactcg acccagagtc accagaaaag aaaaaattaa gccgggaaga
     2281 aatacaactc actatcaaaa aagacgcagc agttaaagac aggacattga ttgagcagaa
     2341 aaccctgtca attcatagca aacttacaga taaatcaatg caactcgaaa aagaaataga
     2401 ctctttttct gcattttcaa acacagcatc tgctgaacag ctatcaaccc agcagaaatc
     2461 attaaccgga cttgccagtg ttactcaatt gatggcaacc tttattcaac tagttggaaa
     2521 aaataatgaa gaatctttaa aaaatgatct ggctctattc cagtctctcc aagaatcaag
     2581 aaaaactgaa atggagagaa aatctgatga gtatgctgct gaagtacgta aagcagaaga
     2641 actcaacaga gtaatgggtt gtgttgggaa aatacttggg gcacttttaa ctatcgttag
     2701 tgttgttgca gcagcttttt ctggaggagc ctctctagca ctggcagctg ttggtttagc
     2761 tcttatggtt acggatgcta tagtacaagc agcgaccggc aattccttca tggaacaagc
     2821 cctgaatccg atcatgaaag cagtcattga acccttaatc aaactccttt cagatgcatt
     2881 tacaaaaatg ctcgaaggct tgggcgtcga ctcgaaaaaa gccaaaatga ttggctctat
     2941 tctgggggca atcgcaggcg ctcttgtcct agttgcagca gtcgttctcg tagccactgt
     3001 tggtaaacag gcagcagcaa aacttgcaga aaatattggc aaaataatag gtaaaaccct
     3061 cacagacctt ataccaaagt ttctcaagaa tttttcttct caactggacg atttaatcac
     3121 taatgctgtt gccagattaa ataaatttct tggtgcagcg ggtgatgaag taatatccaa
     3181 acaaattatt tccacccatt taaaccaagc agttttatta ggagaaagtg ttaactctgc
     3241 cacacaagcg ggaggaagtg tcgcttctgc tgttttccag aacagcgcgt cgacaaatct
     3301 agcagacctg acattatcga aatatcaagt tgaacaactg tcaaaatata tcagtgaagc
     3361 aatagaaaaa ttcggccaat tgcaggaagt aattgcagat ctattagcct caatgtccaa
     3421 ctctcaggct aatagaactg atgttgcaaa agcaattttg caacaaacta ctgcttgata
     3481 caaataagga gaatgttatg gaaattcaaa acacaaaacc aacccagatt ttatatacag
     3541 atatatccac aaaacaaact caaagttctt ccgaaacaca aaaatcacaa aattatcagc
     3601 agattgcagc gcatattcca cttaatgtcg gtaaaaatcc cgtattaaca accacattaa
     3661 atgatgatca acttttaaag ttatcagagc aggttcagca tgattcagaa atcattgctc
     3721 gccttactga caaaaagatg aaagatcttt cagagatgag tcacaccctt actccagaga
     3781 acactctgga tatttccagt ctttcttcta atgctgtttc tttaattatt agtgtagccg
     3841 ttctactttc tgctctccgc actgcagaaa ctaaattggg ctctcaattg tcattgattg
     3901 cgttcgatgc tacaaaatca gctgcagaga acattgttcg gcaaggcctg gcagccctat
     3961 catcaagcat tactggagca gtcacacaag taggtataac gggtatcggt gccaaaaaaa
     4021 cgcattcagg gattagcgac caaaaaggag ccttaagaaa gaaccttgcc actgctcaat
     4081 ctcttgaaaa agagcttgca ggttctaaat tagggttaaa taaacaaata gatacaaata
     4141 tcacctcacc acaaactaac tctagcacaa aatttttagg taaaaataaa ctggcgccag
     4201 ataatatatc cctgtcaact gaacataaaa cttctcttag ttctcccgat atttctttgc
     4261 aggataaaat tgacacccag agaagaactt acgagctcaa taccctttct gcgcagcaaa
     4321 aacaaaacat tggccgtgca acaatggaaa catcagccgt tgctggtaat atatccacat
     4381 caggagggcg ttatgcatct gctcttgaag aagaagaaca actaatcagt caggccagca
     4441 gtaaacaagc agaggaagca tcccaagtat ctaaagaagc atcccaagcg acaaatcaat
     4501 taatacaaaa attattgaat ataattgaca gcatcaacca atcaaagaat tcgacagcca
     4561 gtcagattgc tggtaacatt cgagcttaat atatccaaga gccataataa tatatggctc
     4621 ttcctgtaag gaaataacca tgaatataac aactctgact aatagtattt ccacctcatc
     4681 attcagtcca aacaatacca acggttcatc aaccgaaaca gttaattctg atataaaaac
     4741 aacgaccagt tctcatcctg taagttccct tactatgctc aacgacaccc ttcataatat
     4801 cagaacaaca aatcaggcat taaagaaaga gctttcacaa aaaacgttga ctaaaacatc
     4861 gctagaagaa atagcattac attcatctca gattagcatg gatgtaaata aatccgctca
     4921 actattagat attctttcca ggcacgaata tccaattaat aaagacgcaa gagaattatt
     4981 acattcagct ccgaaagaag ccgagcttga tggagatcaa atgatatctc atagagaact
     5041 gtgggctaaa attgcaaact ccatcaatga tattaatgaa cagtatctga aagtatatga
     5101 acatgccgtt agttcatata ctcaaatgta tcaagatttt agcgctgttc tttccagtct
     5161 tgccggctgg atctctcccg gaggtaacga cggaaactcc gtgaaattac aagtcaactc
     5221 gcttaaaaag gcattggaag aactcaagga aaaatataaa gataaaccgc tatatccagc
     5281 aaataatact gttagtcagg aacaagcaaa taaatggctt acagaattag gtggaacaat
     5341 cggcaaggta tctcaaaaaa acgggggata tgttgtcagt ataaacatga ccccaataga
     5401 caatatgtta aaaagcttag ataatctagg tggaaatggc gaggttgtgc tagataatgc
     5461 aaaatatcag gcatggaatg ccggattctc tgccgaagat gaaacaatga aaaataatct
     5521 tcaaacttta gttcaaaaat acagtaatgc caatagtatt tttgataatt tagtaaaggt
     5581 tttgagtagt acaataagct catgtacaga tacagataaa ctttttctcc atttctgagg
     5641 tgcgccatgc ataatgtaaa taatactcaa gcgccaacat tcttatataa ggcaacttca
     5701 ccatcatcaa cagaatacag cgagttaaaa agcaaaatat ccgatatcca tagttcgcaa
     5761 acttctctaa aaacaccagc atcagtgtct gaaaaagaaa actttgcaac gtcttttaat
     5821 cagaaatgtc ttgatttttt attttcttcc tcagggaaag aagatgtgtt aagaagcatt
     5881 tattccaact caatgaatgc gtatgccaaa agcgagattc tcgaattttc aaatgttttg
     5941 tactccttag tacatcaaaa tggtcttaat tttgaaaacg aaaagggact tcaaaaaatt
     6001 gtcgcacagt attcggaact aattataaaa gataaattat cccaagattc tgcctttgga
     6061 ccatggtcgg caaagaataa gaaactccat caattacgac aaaacattga gcacagactt
     6121 gcactattag cacaacaaca cacatctggt gaagctttat cattgggaca aaaactcctc
     6181 aatactgaag tatcatcatt tatcaagaat aatattcttg ctgaattaaa gttaagtaat
     6241 gaaactgttt catctctcaa actagatgat ttagttgacg cacaggcaaa acttgccttt
     6301 gatagtttgc gcaatcaacg taaaaatact attgatagta aaggatttgg tataggtaaa
     6361 ctgtcaagag acttaaatac agtagccgtg tttcctgagc tgttgagaaa agtccttaat
     6421 gatattttag aagatataaa agattcgcat cctatccaag atggcctccc tacacctccc
     6481 gaagatatgc cagatggcgg accaaccccc ggagccaatg agaaaacatc ccaacctgta
     6541 attcactatc atataaataa tgataataga acttacgata atagagtttt tgacaacaga
     6601 gtatatgaca atagctatca cgagaaccca gaaaatgatg cacagtctcc tacttctcag
     6661 acaaacgatc tattatcccg taacggaaac tcattactaa atccacaaag agcactagtt
     6721 caaaaagtaa cttccgttct accacactct atatcagata ctgtccagac atttgcaaat
     6781 aattcagctt tagaaaaggc tttcaaccat actccagata attcggatgg aataggttca
     6841 gacctgttaa ctacgagtag tcaagaaaga tctgcaaata actctctttc tcggggacac
     6901 aggcctctga acatacagaa ctcttcaacc accccccctc tccacccgga aggagtgaca
     6961 agcagtaatg ataactcatc agatacaact aaaagtagcg cttctctttc tcatagagta
     7021 gcttcgcaaa tcaataaatt caactcaaac actgattcaa aagtacttca gactgatttt
     7081 ttatcaagaa atggagacac atatttaaca cgggaaacga tatttgaagc ttcaaaaaaa
     7141 gtaacaaact ccctaagtaa tcttatatct ctcattggaa ctaaatcagg aacacaagaa
     7201 cgagagttac aggaaaaatc aaaggacatt acaaaatcca caacagaaca tagaataaac
     7261 aacaaattaa aagttacaga tgcaaatata agaaactacg taacagaaac caacgcagat
     7321 acaattgata aaaatcatgc gatctatgaa aaggcaaaag aagtatctag cgccctcagc
     7381 aaggta
//
LOCUS       DAZSNO010000180         7375 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZSNO010000180 DAZSNO010000000
VERSION     DAZSNO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.182"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1541
                     /locus_tag="ACHWN2_003591"
     CDS             <1..1541
                     /locus_tag="ACHWN2_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR8932967.1"
                     /translation="PTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVD
                     TYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGV
                     YSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFT
                     TFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVV
                     DRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKR
                     ILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKG
                     KLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWI
                     TSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVEN
                     NQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKAL
                     SEAK"
     gene            1593..2750
                     /locus_tag="ACHWN2_003592"
     CDS             1593..2750
                     /locus_tag="ACHWN2_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR8932968.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="ACHWN2_003593"
     CDS             complement(2716..2838)
                     /locus_tag="ACHWN2_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932969.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="ACHWN2_003594"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="ACHWN2_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="ACHWN2_003595"
                     /pseudo
     CDS             4764..7124
                     /locus_tag="ACHWN2_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            7181..>7375
                     /locus_tag="ACHWN2_003596"
     CDS             7181..>7375
                     /locus_tag="ACHWN2_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932970.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT         2164 a         1615 c         1567 g         2029 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtccta gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattg
//
LOCUS       DAZSNO010000181         7269 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZSNO010000181 DAZSNO010000000
VERSION     DAZSNO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7269
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.183"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1077
                     /locus_tag="ACHWN2_003597"
     CDS             <1..1077
                     /locus_tag="ACHWN2_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HFR8932971.1"
                     /translation="AAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLHDAYKASDERLYVNKQNKNSRS"
     gene            1120..1623
                     /gene="yeaK"
                     /locus_tag="ACHWN2_003598"
     CDS             1120..1623
                     /gene="yeaK"
                     /locus_tag="ACHWN2_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR8932972.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1624..1728)
                     /gene="yoaI"
                     /locus_tag="ACHWN2_003599"
     CDS             complement(1624..1728)
                     /gene="yoaI"
                     /locus_tag="ACHWN2_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR8932973.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1898..2344
                     /locus_tag="ACHWN2_003600"
     CDS             1898..2344
                     /locus_tag="ACHWN2_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR8932974.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2301..3119)
                     /gene="nimR"
                     /locus_tag="ACHWN2_003601"
                     /pseudo
     CDS             complement(2301..3119)
                     /gene="nimR"
                     /locus_tag="ACHWN2_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3216..4396
                     /gene="nimT"
                     /locus_tag="ACHWN2_003602"
                     /pseudo
     CDS             3216..4396
                     /gene="nimT"
                     /locus_tag="ACHWN2_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4451..4798
                     /locus_tag="ACHWN2_003603"
     CDS             4451..4798
                     /locus_tag="ACHWN2_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR8932975.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4820..5074)
                     /gene="yoaF"
                     /locus_tag="ACHWN2_003604"
     CDS             complement(4820..5074)
                     /gene="yoaF"
                     /locus_tag="ACHWN2_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR8932976.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5257..6282
                     /gene="dgcP"
                     /locus_tag="ACHWN2_003605"
     CDS             5257..6282
                     /gene="dgcP"
                     /locus_tag="ACHWN2_003605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR8932977.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6315..6413
                     /locus_tag="ACHWN2_003606"
     CDS             6315..6413
                     /locus_tag="ACHWN2_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR8932978.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6416..6490
                     /gene="yoaJ"
                     /locus_tag="ACHWN2_003607"
     CDS             6416..6490
                     /gene="yoaJ"
                     /locus_tag="ACHWN2_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR8932979.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6651..7028)
                     /locus_tag="ACHWN2_003608"
                     /pseudo
     CDS             complement(6651..7028)
                     /locus_tag="ACHWN2_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(7022..7138)
                     /locus_tag="ACHWN2_003609"
     CDS             complement(7022..7138)
                     /locus_tag="ACHWN2_003609"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8932980.1"
                     /translation="MRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT         1753 a         1763 c         1771 g         1982 t
ORIGIN      
        1 gctgctgagc agtcggaagt gtcgccagaa cagcggcgcg ccatctgcga ggcttttgag
       61 tctgccaata atacccatgg cttaaacctg actgcccata aatacccggg cttacgcggc
      121 acactacaaa ccgcatccac tgactgcgac acaattgtgg aagctgcagc actattaccc
      181 gcttttgatc aggcagtgga aggcaaccgc caccaggatg attacggttc aggtcttggg
      241 atggccgaag agaaatttca ctattatctc gatctcaatg accgctatgt ctatttttat
      301 gagccggtta atgttgaata ctttgcgatg aataactggt ccttcctgca gtcaggaagt
      361 attggcatcg atcgcaaaga tattgaaaag gtatttaccg ggcgtaccgt attgtcgagc
      421 atttaccagg atcagcgtac taaacagaac gtgatgagtt tgctgacgcc ggtatatgtc
      481 gcagggcagc taaaagggat tgtgctgctg gatattaaca aaaacaattt gcggaatatc
      541 ttttacactc atgaccgccc tctcctctgg cgttttctca atgtcacgct aaccgatacc
      601 gattcggggc gcgacattat catcaaccag agcgaagata atctgttcca gtatgtcagt
      661 tacgtccatg acttacctgg cggcattcgt gtctcgttat ccattgatat tctttacttt
      721 atcacgtctt cgaggaaaag cgttctgttc tggattttga cggcgttaat tttgctgaat
      781 atggtccgga tgcacttccg tttataccaa aatgtgtcgc gagaaaatat tagtgatgcg
      841 atgactggac tgtataatcg caaaatttta acccctgaac tggagcagcg gttgcagaaa
      901 ctggtgcaat ccggttcttc ggtgatgttt attgcaattg acatggacaa gttaaagcaa
      961 ataaatgaca ccctcggtca tcaggagggg gatttagcga ttacgttaca tgatgcgtat
     1021 aaagcttccg atgagcgttt atatgtcaat aagcagaaca aaaacagccg ttcatgataa
     1081 ccttctgtgg ttgtttgctt gtaatctcag gagcgtgaaa tgactgaaat ggctaaagga
     1141 agcgtgacgc atcagcagtt aatcgcttta ttatcacaag aaggtgctaa cttccgcgtc
     1201 gttacccatg aagcggtagg taaatgtgaa gcggtgtcag aaattcgtgg taccgctcta
     1261 ggccagggtg ctaaggcact ggtatgtaaa gtcaaaggca atggcgtaaa tcaacatgtt
     1321 ctggcgatcc tcgcagccga tcaacaggcc gatctgagcc aactcgccag ccatataggc
     1381 ggattacgcg cttcgttggc cagcccggcg gaagttgatg aactcacagg ctgtgtcttc
     1441 ggcgcgatcc cccctttcag cttccatcca aaactcaaac tggttgccga ccctctactc
     1501 ttcgaacgat ttgatgaaat cgccttcaat gcagggatgc tggataaatc cgttattttg
     1561 aaaaccgcgg attatttgcg cattgcgcaa ccagaactgg tcaatttccg ccgcactgcg
     1621 tagctagcct atccgttcaa taagcagaac ggacaaaacc agcacaaccg caatagcaaa
     1681 aaacgatgat gtgataatca gtgtctcgac gaacatttga tcgttcatag catgcccccg
     1741 ggagatagct gttttcacgt tattgttagc gtgcacaaat ggcagtttga tgacagttcg
     1801 cctgattttt tatgcaaaaa acgtaaagat tttctacttc cttcctgcag caagcataaa
     1861 gtaagcaggc ttattatttt ttggcaagga aaccacgatg tttgatgtca ctttgctgat
     1921 cctgctcggg ttagctgcgc tgggctttat cagtcataac accactgtcg ccgtttcaat
     1981 tctggtgttg atcattgtcc gcgtcacacc gttaagcacc tttttcccgt ggattgaaaa
     2041 acaggggcta agtatcggga taatcattct gacgattggc gttatggccc ccatcgccag
     2101 cgggacgcta ccgccctcaa ccttgattca ctcgtttctg aactggaaat cgctggtggc
     2161 aattgcagta ggcgttattg tctcctggct gggtgggcgc ggcgtgacgt taatgggcag
     2221 ccagccgcaa ctggttgctg ggctgctggt gggcactgtt ttaggcgtag cgctgtttcg
     2281 cggcgtaccg gttggcccgc ttattgccgc gggtctggtt tcgctgattg tggggaaaca
     2341 gtagttaatc ccgcaatata tcggcctggc gtttgcccca agcctttctt aaacatagta
     2401 ataaaggccg ttgttgaatc ataccccagc gtatgagcaa ctttctgtac agtatcgcct
     2461 ttcactaatc cctgaagcgc cataatcagc tgtaactgct gacgccactg gcgaaaactc
     2521 aaccccgtct ctttcactat caggcgggca agattgcgtt cactcatcgc aaaaaagcca
     2581 gcccattgcc ccaatacccc ccactcgacg ggccctttcg ccatcatctc caccatcgtg
     2641 cggattttag gatgagaaga aacgggcagg tgcagctgtt gttgtggctg ctgagggagt
     2701 tcatcaaaca ggacctgaat taatctgcgg gtcatcggct cagcacgctg agtcgtcgtc
     2761 ctgtttgcca gggttaaaat caattcccgg cataagggcg atattttcaa tgtgcaacat
     2821 ggtcggcatt gtcactgcgc tgggttcgat aaacagaaaa cagagttcag cgttagctgt
     2881 cacttgattg ctatgcttca ccccgcccgg tatccaaacc gcatattgtg gcggcaccat
     2941 ccacaaagca ttttccaccg tacaggtaat tgcaccatgt aatgccagta tcagctgtcc
     3001 tttgcggtgc tgatgtacag gactggttaa ctcatccgtt ccggcgtgaa tacaaaatgc
     3061 gaccgcggct tcatggtgtc ggtgggttca tagccattga gattcaacct gtgcatcatt
     3121 ttgtccgaac ttagcgataa tttgtcattt tagcttgatt caacataaca ataaaaacgg
     3181 taaggtacag cctcgtttgt aacaatgaga agcatatgac ctgttcaact tcattaagcg
     3241 gcaaaaacag gattgtcctt atcgctggca ttctgatgat tgccacaaca ttacgcgtca
     3301 cctttaccgg cgcagcgccg ttactggata cgattcgttc cgcttactcg ctgacgacag
     3361 cgcaaaccgg cttattaacc accctgccat tattggcctt tgcgctaatc tcacctctgg
     3421 ctgccccggt ggcgcgacgt tttggtatgg aacgtagcct gtttgccgcg ttacttttga
     3481 tctgtgcagg tatcgcaatt cgttctctcc cttcgcctta cttattattt ggcggtacag
     3541 cggtcattgg cggtgggatt gcattaggca atgtcttact gccaggatta attaaacgcg
     3601 atttccctca ttccgtcgcc agacttaccg gcgcatattc cctgacaatg ggagctgcag
     3661 cggcactggg atcggctatg gtcgtgccgc tggctttgaa cggttttggc tggcaaggcg
     3721 cgttgttcat gctgatgtgt tttcctctgc tggctctttt tttatggctg ccacagtggc
     3781 gaagtcagca acatgcaaat ttgagtacct cacgcgcctt acatactcgg ggtatctggc
     3841 gttcgccact tgcctggcag gtaacattgt ttcttgggat caactcactg gtctattacg
     3901 tgattattgg ctggcttccg gcgatcctca tcagtcacgg ctatagcgaa gcacaggcgg
     3961 gttcactgca tggtttgctg caactagcca cagcagctcc cggtttgctg atcgcacttt
     4021 tcttacatca tgtgaaagac cagcgtggta ttgcagcgtt cgttgccttg atgtgcgcag
     4081 tgggcgcggt tgggctctgc tttatgccag cgcacgcgat cacctggact ctgcttttcg
     4141 gttttggttc cggcgcaaca atgatactgg ggttgacgtt cattggtctt cggcaagttc
     4201 tgcgcatcag gcggcggcac tctcggggat ggcacaatcc gtcgggtatt tgctggcagc
     4261 ctgtgggccg ccgctaatgg gtaaaataca cgatgctaac ggtaactggt ccgtaccact
     4321 gatgggtgtt gcaatacttt cactactgat ggcgattttc ggactttgcg ccgggagaga
     4381 caaagaaatt cgctaatatc cggtgctata gtgacgtgac aaatcttgcg tgaaagggag
     4441 aacaaacacg atgaatattc agtgcaaacg cgtttatgat ccggctgaac agagcgatgg
     4501 ttatcgcgta ctggtcgacc gcctctggcc gcgcggtatc aaaaaaaccg atttagccct
     4561 tgatgagtgg gataaagaaa tcacgccgtc aacggaactg cgcaaagcct ttcacggcga
     4621 agtcgtcgat tttgcaacct ttcgcgagca atatcgcgca gaactggcgc aacacgagca
     4681 agaaggaaag cggctggcgg acatcgccaa aaaacagccg ctgaccctgc tctacgcagc
     4741 aaaaaacacc acgcagaacc atgcgctggt gctggccgac tggctacgta gcttgtgatt
     4801 ttagtacggc atccggcggt tatttttcac cagccggatg gtcccgtcgc cacaatgccc
     4861 attcatcaat cgtttcaccg cccggtaatt tgcaattgtt gctgacccct tgcgctgtct
     4921 gcactggaat gagcgtcccg cccttctgct ggcaatagac cgacgccgga tttgccatac
     4981 caatctgcgg cggtttaggt gcttctggct gagaaggggt tgaacaacca gccaggacca
     5041 gcaagcaagg cagaacaaaa ctgataattt tcatttattg atctcacata tttatccaag
     5101 cttagagtat cgcggtatcg ttttgttttg cagcactatt tttattacat tcactcaaaa
     5161 catattacgt cttgtttcat ctttgttgat gatgttttat catgcctgca aagattaaat
     5221 aatcagcact tacccgccgt atcctggagt tgttccgtgt cagatcagat tatcgcccgc
     5281 gtctcgcaat cccttgccaa agaacagtca ctggaaagtc tggtccgaca gcttctggag
     5341 atgctggaaa tggtcactga tatggaatca acctacctga ccaaagtgga tgtcgaagcg
     5401 cgcctgcagc atattatgtt tgcccgtaac agccagaaaa tgcacatccc ggagaatttt
     5461 accgtcttgt gggattactc gttatgcaaa cgcgccattg atgaaaactg ctttttcagc
     5521 gatgaagtcc ctgaccgttg gggcgactgt attgcggcac gcaatcttgg catcaccaca
     5581 tttctgagca cgccaattca cttaccggat ggatcattct atggcacgct ttgcgccgcc
     5641 agcagtgaga agcgccagtg gagtgaacgc gcggaacagg ttttacagtt attcgccgga
     5701 ctgattgcac aatatattca aaaagaggca ctggttgaac agctgcacga agccaatgct
     5761 gcgctgattg cgcaatcgta taccgactcg ttaaccgggc taccgaatcg gcgggcgatt
     5821 tttgaaaatc tgacgacgct gttttccctc gcccggcatc ttaaccataa gataatgatc
     5881 gcgtttatcg atctggataa cttcaaatta atcaatgatc gttttggtca taatagtggc
     5941 gatctgtttc tcattcaggt tggcgagcgc cttaatacgc tccagcaaaa tggcgaagtt
     6001 attggtcgtc tcggtggtga tgagtttttg gttgtttcac taaacaacga gaatgcggat
     6061 atttcgtcgc tgcgagaacg tattcaacag caaatacgtg gagaatatca cttaggtgat
     6121 gttgatttgt attatcccgg tgccagtctt ggcatagtag aagtcgatcc tgaaacaacc
     6181 gatgcagaca gtgccctgca tgctgccgat attgcgatgt atcaggagaa aaaacacaaa
     6241 cagaaaacac cttttgtcgc gcatccagcg ctacattcct gaggcgtatt cacatccttt
     6301 tgattggtga taacatgcga atcggtatta tttttccggt tgtaatcttc attacagcgg
     6361 tcgtattttt agcatggttt tttattggct gctatgctgc cccgggagca taaagatgaa
     6421 aaaaacaacg attattatga tgggtgtggc gattattgtc gtactcggca ctgagctggg
     6481 atggtggtaa cgtcacctct aaaaaatagc aaaggctgcc tgtgtgcagc ctttgtgcaa
     6541 tttaagcgtt aacttttaat cttcctgtag ataaatagca cgacaatcgc accaataacg
     6601 gctgaagcgg cgcacgaaaa acgctttcgc gtttttcgtg cgccgcttca cccctggttc
     6661 agcatttcgt ctgcagtctg aaccgcaggc aaccattttt cagcaagtct gcgctgggaa
     6721 tcgctggctt tatcccattt gctccacagt gcctcacgat cgtaatcgtg cgcttccagc
     6781 gtggggcggg cgatttcaaa atacccctag cttgtttcta tttgctcaga ttttaataac
     6841 aactcagcac gtgctgccgc tggtagaact gatatatgaa attccaaagc ctttgaaccc
     6901 gtacgcttgc gacgtagttc tggatgattg gctgcaagct tttcaagatt gcggcgaata
     6961 ctcacctcat gtttaggaaa gcatgacatt cctacgcatt cttgcgctgt tacccaatgg
     7021 gttaacatga tgttttctcc atataccgag aaggccaaat ctgggctggt tccatatcca
     7081 aatatctagc aatgattgct tcgtatcgtg gtatatggca gcgaaatacg ttacgcatag
     7141 tgtcaggctt taagcctgcc ttgaggtgta ctggcaatag cggatactac catttgttct
     7201 ttttttaagc agttatctga tgatattttt ccctgaaggc tgccggggag atattcccca
     7261 gacgagagt
//
LOCUS       DAZSNO010000182         7258 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZSNO010000182 DAZSNO010000000
VERSION     DAZSNO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.184"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="ACHWN2_003610"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="ACHWN2_003610"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR8932981.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACHWN2_003611"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACHWN2_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR8932982.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACHWN2_003612"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACHWN2_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR8932983.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="ACHWN2_003613"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="ACHWN2_003613"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR8932984.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="ACHWN2_003614"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="ACHWN2_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="ACHWN2_003615"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="ACHWN2_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR8932985.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="ACHWN2_003616"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="ACHWN2_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR8932986.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2057 a         1647 c         1519 g         2035 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actttggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
     6901 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6961 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
     7021 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
     7081 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
     7141 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttatat aaaatcggta
     7201 acggctggaa atcattcaat actcgcacta tcgaaagttt accagccaac cgcagcac
//
LOCUS       DAZSNO010000183         7183 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZSNO010000183 DAZSNO010000000
VERSION     DAZSNO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.185"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(371..1198)
                     /locus_tag="ACHWN2_003617"
                     /pseudo
     CDS             complement(371..1198)
                     /locus_tag="ACHWN2_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHWN2_003618"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHWN2_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFR8932987.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHWN2_003619"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHWN2_003619"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFR8932988.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHWN2_003620"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHWN2_003620"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFR8932989.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHWN2_003621"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHWN2_003621"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFR8932990.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHWN2_003622"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHWN2_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="ACHWN2_003623"
     CDS             7090..>7183
                     /locus_tag="ACHWN2_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HFR8932991.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAZSNO010000184         7055 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZSNO010000184 DAZSNO010000000
VERSION     DAZSNO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.186"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(66..1901)
                     /locus_tag="ACHWN2_003624"
     CDS             complement(66..1901)
                     /locus_tag="ACHWN2_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932992.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            2002..2289
                     /locus_tag="ACHWN2_003625"
     CDS             2002..2289
                     /locus_tag="ACHWN2_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8932993.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            2261..3790
                     /locus_tag="ACHWN2_003626"
     CDS             2261..3790
                     /locus_tag="ACHWN2_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFR8932994.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(3960..5177)
                     /locus_tag="ACHWN2_003627"
     CDS             complement(3960..5177)
                     /locus_tag="ACHWN2_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8932995.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(5544..6530)
                     /gene="ycdU"
                     /locus_tag="ACHWN2_003628"
     CDS             complement(5544..6530)
                     /gene="ycdU"
                     /locus_tag="ACHWN2_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFR8932996.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(6527..7055)
                     /locus_tag="ACHWN2_003629"
                     /pseudo
     CDS             complement(6527..7055)
                     /locus_tag="ACHWN2_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         2157 a         1651 c         1323 g         1924 t
ORIGIN      
        1 gctaatggtt agcaaaatta gcagggtata taaagattat tgacccaaaa cacactcaga
       61 tgaattcacg acagctcagg agggttattc ctgatgtcat cttcacaaat aatggttaac
      121 gcatcatata tcattctgcg tctgttcctg ttttcggcct tatcgccata caacatcatc
      181 acatttttaa tttcatcgac cggtgatttc acatagtgaa ctaatacacc aagcttctca
      241 acacattgca gtaaagtata tttcttactg cctgtgaaaa gccctatcat agtttcttta
      301 cgttcgcgac tggataatac tttgttttcc ataatgactt ctgccagcgt cttaccatca
      361 taaacatact ctttatctat cagaatattt atcttatcca gcaccttctc tacgtcatca
      421 agcaaacctg taatatatgg gggcgcaata tatggtttcg tactgatctt ttcaagcata
      481 caattataca tcttaataac acgcatcagg gtaaaattgt tcgcatcata ctgtatttca
      541 ccctccttat atacaggatc aataagtggt attttttcaa taaatgcatc ctgataggct
      601 tttacatggt caagccaata aaacaacgag ccatacttta tattttttct atttacttta
      661 acctccagtg ctgtccgaag caacgataat gcccccctga catatcctga aggtaattca
      721 ccaaacaatc cttttctttc catgtcatca taatgctgca gcacatcatt aattttattt
      781 ttccttatca gacaaatgta caccgcaagt gtttcgtagc agctggtcac atgaacagcc
      841 tcattctgca actgcccaaa atagcttata atctttttca gatatgcgtc atcacaacca
      901 ttcttatcat taatattaaa tacaagatct ttcagacggg aaatatattc ctgagattta
      961 tcatcaagtg aaacactata acgagacaat gttccttctg cactgttttg aaacttataa
     1021 aacatttcca gtaatttttt tgcataaggc gttgtatcaa agctggtaaa gtcataaaga
     1081 tattgcgcag cgtaaccata caaaccatga atccagagtc cttctctcac caacactctg
     1141 agataatatg caaggctttg aagtatcttc gtctttcgtt gtatatcaag taagacactt
     1201 aatgaataaa atgcattata gaatttatca atattcctga gcactttctc cagttcatcc
     1261 ttatccagct tcttctcccg gtaatccttt ttatattcat ttttgatagc catgatatat
     1321 tgttgcatca ggctcttgct tttcccgtta tccttccgta tagttcctgt atatttcccc
     1381 agagcataca gctttccatt ttcatgaaaa tcttccttgt tgaatcccag ttcagccaga
     1441 tattcccgaa gataacgttt aacagcagct tcatcgcttt ctgtatcagc gtgatcaaga
     1501 caaggacaat acgatttctg taaaaaatta taaatatgat aataaaaagc agagtcattc
     1561 tctggtctgt atgtattata catatctaca acaagaaaaa taaccccaaa agtgcgcaga
     1621 caatagctgc gaaggaattc aggttttagt gtggaatgca ctggataaga attcatcatg
     1681 taataaaaat gttcatatcg ggcatatatg atccgccgtt gctcctgact gaacacccct
     1741 tcagcatcaa taatacgact gatctcatta taacgttgat cccagaattt ttgatgcatt
     1801 tcaaaattgg tactcttccg cttgtgtgcc ggattctcgc cttccttaaa caaccccaaa
     1861 acagtacaaa tatcatacaa catatttgta aatgtgcgca tatgctgtgc tctccggttg
     1921 gtttgcattt tatcgcagag gacattctga gcttaacgta tcaaactgac acaacgacat
     1981 gcaatgcata tggagaacaa tatgtcaaat caaaccggac agctttacac gccttcacgc
     2041 atcctgcgta tggctgaact tacccgactg ctgggtatat caagatctac tatttacgtg
     2101 aaaataaatc cggcctcgaa ataccacgac cctacctttc ccaaaccaat aaggcttggt
     2161 agtgcatccg taggctggcg ggcatctgcc atcgatgagt ggctgatgtt acatacggca
     2221 ccagcctggt ctgaacctga aaacaaacta aaggaactta atgatgacga caaaactatc
     2281 actccctgaa cttgacacaa ctaaactgcc tgacaggtgc caggcattac ttgatgaaat
     2341 gcatgaagaa acaggaatac gccgtgaaat acttctgtct gttatgctga cggtcatggc
     2401 tgcctcggtc caggatacgc atgaagttga actttccgga gggcaacgta ccagccttca
     2461 gatatttatg tgtctttcat cagcatcagg cagtggaaaa acctctgcct gcgcaaaatt
     2521 aatcgctcct gttcatgaaa cagaagaaga actacatcag gcatatatcg acgataaaaa
     2581 aaactatgac cgaatgatgg aaatgtggac aacagataaa aaaatcctgg agcggagata
     2641 taaaaaggaa attgaaagat ccccggaaaa tgctgctgct gcacgagcag cacttgaaaa
     2701 gtgtatagca aataaacccg aacctcccgt acaacaggtt ctcatcgtga atgacgcgac
     2761 accagaaggg atagctttga aactcagcca gtcaccctct cttctgttgc tgtctgatga
     2821 aggaggtact attctggata agcgtttcga acgcaaatcg gcactgtaca acacattatg
     2881 gagtgggcaa ccagtcaacg ttgaacgggc atccagaccc gggttccggc tgaatgatgt
     2941 ccgtctcact atacttatac tgactcaacc ggtaatattt aataagtttt ttactctcac
     3001 tggcgatcag atccggggca atggctttct ggcccggttg ctattttgcg aacccggagc
     3061 aattaaaata atgacgacag aaccgcatac tgccactcct gttgtgacgc aacaatgtgc
     3121 aagctgtttt cgtaagaaaa gttttggcag tcagatcaga gactctctga gagcatccag
     3181 agaaagacgt gcaaaaagta aagagcgcat ctgcatgaca ttatcaaatg cagcatcccg
     3241 cgcactggag cgctttcaca aagaaaatat ggatactgtc agacagaatc ctcatatggg
     3301 tacatttgaa gacattattg tcagaaaaag agagcaggtt gtcaggatag cagcacttct
     3361 ggaactggag aaagatccag actcgacagt aataacgctg gaaagtacta atagcgccat
     3421 ttatctcatt gaattttatt tcaagcatct tatatataaa cttgaatccc ttcgggaaat
     3481 atccccggct gagaaacttg ataaatggct tcagaaaaga attatcacaa cagcaggtta
     3541 catattccag aaaagttata ttttacaata tgccccatat gctctcagga aaaaatgtgt
     3601 tctggatgaa gcacttgaca tactggcgga acaaaggaaa ataaggattg atgataacct
     3661 ggttgtttat ataggaaaca cgattacacc atccgaactg gcaaaaaaac taaatattcc
     3721 tgcattcgat gccggagttt tcatttgtga tcatcaaaac atactgaagt atcaccgaaa
     3781 cagactatag atactatcgg acaaaaaata aattatcaca agtgtattat tcgttataat
     3841 aatgaataat cgttatctga gtgcaatgcc aggttttcat tacgaactgg cattgcattt
     3901 atttttcaaa aaagaaagca gtaaccattt ggctactctg agtatgccag tccatgaaac
     3961 taagcgaatt ccttagtgtt tttagcaagt tgagacatca cataatccgc ccatgcctgc
     4021 atcatcggta cacgctgctc cagtagatca gtacgatgat atgccgcctc aaccttattt
     4081 ttcagcgtat gagcgagcgc cctttccgcc agatcccgcg aatacccctg ttcactacac
     4141 cagtccctga atgttgagcg aaaaccatgt gccgtggcaa ctcgccccgg aatgtcactg
     4201 acggctttct ttttacgcag aaaacttgtc aacaccatat cggaaaggat ctgctgcttt
     4261 ctgggtgaag ggaacaccag ttcatcatgc aggccacgta tattttccag aatgtaaata
     4321 gcctgccggg ataaaggaac acgatgctgt agcctagctt tcattctttc tgcaggtata
     4381 gtccataccc gcttatgaaa atcaatttca gcccagcgca ttcccctggc ttcgcccgag
     4441 cgagttgctg taagtatcac cattaataac agtgcgcggg taacattata aggttcatca
     4501 ctatacacac tggtcgccac aaaaagcggt aactgcctcc agggcattgc gggttggtgt
     4561 tcatcacgtc ctcttgtctg ctgaggaagc aaatggtcaa ccacatcaac aggatttgcc
     4621 acacaaaaac cgtgcgccca tccccactgc ataacaacat gaatgcgctg tttaacccgg
     4681 cttgccgttt ctgacaaggt taaccagact ggacgcagtg tttctgccac gtccgcagcc
     4741 gtaatcgaat ccagcgtttt tgctcccagt tgaggaaacg cgtaattctc aagcgtcgat
     4801 aaccactgcc ttacatgctt tggattctcc catccaggag acagttctgc atgtacacgc
     4861 ctggctgcat cggcaaatgt tgggataacg actttctcag attcagcctt tttaatctcc
     4921 agaggatcat cacctgcagc aagttgctct cgcattatcc gggcagtacg tgcagcttca
     4981 gcaatactga cctctgggta agttcccaat ccagcattac gtcttttttg tgtcaccgga
     5041 cttacataac gaaaaaccca tttcccccgc ccctttactg aagaaggatg aagggtcagt
     5101 ccggtaattc ccccatgggg caatggtttg tcatcaggtt tgatatgtct tgctttcgta
     5161 tccgtcaata ctgccatacg ctaattcccg tcactctggt atgccatcca gtatgccatt
     5221 agtccctcgc ttcactaaga atacattaga taacatcgga caacaaaaag tgtaatacat
     5281 tgtttataaa gaaattatat aacacccctg gattaagtca gatttatttc aggcggtccc
     5341 cctcaccgcc atatttaaag aagagcgcgt acgaaagtac gggctttttt ttcgtatatt
     5401 gtacacaccg ggtgcccccg cccacgtctg ttagggcgag gggaaattgt gcacatcaac
     5461 tgtctggtta ctcagtcaac aatcaaaatg cgtaagatag tacagaacat atatgcgtta
     5521 tctggtcact tcctgtaact tcctcactta ttaaaatgcc cccaacggaa atacatgtca
     5581 catgccatca taaaatcgtt atgccagagg aatcgacaat ggaaaaaata gtaaattaag
     5641 cacggtacat agcagagtaa aaaataggct tcaaaccaaa tccgccattc cacaagcgtt
     5701 gccaataccg acaccagcgt aatccacatc atttcaataa cccgcaatat gaataaccga
     5761 agccaaagcg gcttggcaaa aaaggtcgac aacgtatttt cactggagaa aaacagatcg
     5821 atttcctgca aaaaaaacct gcccagaaaa gcgggccgct tggagaagcg cagaaaaaac
     5881 agattaatcg ccaacactac gccacaaaga atgagcaatg caattatcga tttaaacgtt
     5941 aaccactgcc cctctgtcac aaaagaatca ctgttacgca gcgggctgat agtaagcagg
     6001 ttaacaaccg ttgagatgca caccgaaagc agcacatagc gagtcagact tttatatttt
     6061 acttttttcg ctactgaaat agcgacctgc ttgacataac tggtgcggat catccgaaac
     6121 agcagaaaga tgaccaacgg cccaagtaac gaaatcagga acagcgcata taacgagggt
     6181 agaagctggt atagccccca actcattgca ctcaacatga ttaacgacag aaatcctgcc
     6241 accagaggtt gtgtaagaaa taattctatt ttgtggttct gctttttctt aaaccacgaa
     6301 aaatcgatat tcttcgtgac gcatttataa gcccagtacg ccttgagatc aaaaataaaa
     6361 ttatacagca ccagggccag aatcccacta actacaccaa tatcctctga tggaaaagta
     6421 aaccctgcat ttttccaata aaagcctatc agaataagtc caatagcata aatgtatatc
     6481 agatttcgtt tatcctgaaa gcgaataaaa gttaaataat caggaatcat ttgaatacct
     6541 ttgcatcact aaatgcttta cgacaatgca taactcgttg ctgatccacg ccagcaaaat
     6601 catttaacat attccccaaa tagggcttat tacttatttc aaggaaagct accggggttg
     6661 gcatattcag aatatgtatc gagagattca gtccatttca ccgtatgagt taagtgtaat
     6721 gagagatttt ctttgataat tgacggagcg acctcgcttt ttgctgttac gttcatcaga
     6781 acctgatgct ctggcgaagc gatatccagt ccggcaagat aatcgcgcat tgcctgaacg
     6841 ccgtcttcca tcaaacgcgt gtgccaggca ccgctcacac cgagtttaac cggttcgtat
     6901 ccagcggcca tcagcagcgt ggtaaattca ttcaacgcag cctgcgttcc cccaataacc
     6961 tgctggcgcg gcgtgttatc acagctaata tccagcgcaa tgcctgattc cgtaatcatc
     7021 gtctgcagtt gttcgcggtt gatgccttta accgc
//
LOCUS       DAZSNO010000185         7022 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZSNO010000185 DAZSNO010000000
VERSION     DAZSNO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.187"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..146)
                     /locus_tag="ACHWN2_003630"
     CDS             complement(<1..146)
                     /locus_tag="ACHWN2_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8932997.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSL"
     gene            224..1204
                     /gene="ddlA"
                     /locus_tag="ACHWN2_003631"
     CDS             224..1204
                     /gene="ddlA"
                     /locus_tag="ACHWN2_003631"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR8932998.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINTLPGFTNISMYPKLWQASGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1228..1440)
                     /locus_tag="ACHWN2_003632"
     CDS             complement(1228..1440)
                     /locus_tag="ACHWN2_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR8932999.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1700..2008
                     /locus_tag="ACHWN2_003633"
     CDS             1700..2008
                     /locus_tag="ACHWN2_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR8933000.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2067..3161)
                     /gene="yaiW"
                     /locus_tag="ACHWN2_003634"
     CDS             complement(2067..3161)
                     /gene="yaiW"
                     /locus_tag="ACHWN2_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR8933001.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3174..4394)
                     /gene="sbmA"
                     /locus_tag="ACHWN2_003635"
     CDS             complement(3174..4394)
                     /gene="sbmA"
                     /locus_tag="ACHWN2_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR8933002.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4746..5903
                     /gene="ampH"
                     /locus_tag="ACHWN2_003636"
     CDS             4746..5903
                     /gene="ampH"
                     /locus_tag="ACHWN2_003636"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR8933003.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5904..6407)
                     /locus_tag="ACHWN2_003637"
     CDS             complement(5904..6407)
                     /locus_tag="ACHWN2_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8933004.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6495..>7022)
                     /locus_tag="ACHWN2_003638"
     CDS             complement(6495..>7022)
                     /locus_tag="ACHWN2_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR8933005.1"
                     /translation="SLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGD
                     AGYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTP
                     YFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGD
                     VDQDWSANVGVKYTW"
BASE COUNT         1780 a         1802 c         1690 g         1750 t
ORIGIN      
        1 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
       61 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
      121 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
      181 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
      241 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
      301 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
      361 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
      421 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
      481 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
      541 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
      601 gtttgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg tcaccaaacg
      661 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
      721 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
      781 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
      841 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
      901 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
      961 gatcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     1021 gaaagtggta gttccggcag ccattgcacc agagatcaac acactgcctg gcttcaccaa
     1081 catcagtatg tatccgaagc tgtggcaagc cagcggtctg ggttacaccg atctgatcac
     1141 acgtttgatt gagctggcgc tggagcgtca cgctgcggat aacgcattga aaaccacaat
     1201 gtaatatttt aacgcccgat aatattctta ttgggcgttt tcagtttctt catcgcgacg
     1261 acgaataatc aaccccgcca gccagaaact aatcacccac gtcaccagac cgacggcata
     1321 ggtctgccaa ccttttgctt caaatcccag tacccccacc acgccattaa gaatgaatat
     1381 aaggccaatg gcgaacgcat agtagtgcca gtcacggcgg atttttacag gcagattcat
     1441 ggcagctccg ttaacgaaaa acaacatcct tgcatattct gtaacaaccg tctgtggtgt
     1501 agacaacatt ttcgtaatta ttaataaatt taacttaagg ttgccatttt gtgactcaca
     1561 acgaagattc acgcttcggg aatattccca acatcaattt accatgattc tgatattgac
     1621 aatctaaatg acctgtcaga cttgccataa gccgctggta tcacaccaga agagaagcag
     1681 atgacttccg gaggttttta tggctgattt caccctgtca aaatcgctgt ttagcggaaa
     1741 atatcgcaat gcctcttcaa cgcctggcaa cattgcctat gcgttgtttg tgctgttttg
     1801 cttttgggct ggggcgcaat tgctgaacct gttagtgcat gcgcccggcg tctatgagcg
     1861 tttaatgcag gtccaggaaa caggtcgccc acgggtggaa attggtttag gtgtcggcac
     1921 cattttcggg ctgatcccgt ttttagcagg ctgcctcatt tttgcagtgg tggcgctatg
     1981 gctgcactgg cgacatcgcc gccagtaaaa tttacgttgc cagatgcggc ctgaaacgcc
     2041 ttatctggcc tgacattcct gtttgatcac tgtttcatac aacgcgcccg ccgttcgtct
     2101 acgcgcttcg caaaccaggc cgtagtcagg ttgcgggtga ttttcgggct ttccagttga
     2161 atgccaggca acatttcgcg gggtagtgat ttacccgttt tcgcttcggc aagttgataa
     2221 actttcttgt acagcgccgt ctcctcaaaa gagaagctgt cacctttctc taactgacgg
     2281 cgaatttcgc tgtcgctcat ccccagtttt cccgccagtt tgcgcgttgc cagttcggtt
     2341 ttcccgggtt ctttactgtc gtaacgaatt aaatcaccgt ccagtgccag cttcacgccg
     2401 ctggccttac tgactgcgtt ctgaaacgcg gcattacggc tggcgtacca gccagcgtta
     2461 aaatcagcaa agcggtatat cggtgcgcta tagctggcgg gataattcag taaatggtaa
     2521 gtaccaaacc acaacccgcc gcggcgactg aagacttcct gacgaactgt accgtccatt
     2581 ttccacggat accctttggt atgctgttcg gcaaaagcaa tgctgacctg catcggccca
     2641 ccggtgcgca ccggattaag cgaaccaaac agcgtctgcc ccattggtac catgctgatc
     2701 agatcgtcaa aaatcgcgct caattgcttt tctgtacgca ccgaatctaa ccgttcgcta
     2761 taacttttgc cgtttggcga tttgattttt agcgcggtat gaaccagaaa agcaggaatg
     2821 tgcatccgtt cagcacgacg gtcaatctct tgccaggcga tcttgcttaa gcccggaacg
     2881 gccggatctg cctgataatt tgactcctgc tgcgccaccg ccagcaccga gtagacgttt
     2941 tccagcgtcg gtgccagccc ctgactttca aaggtggtgg cgagatcttt cgcccaggca
     3001 tcgcggtctt tcacgctcgc aggcatcttc tgccgcacga cgctcgctac atctatcgct
     3061 ttttcgccct ttttcaacgg ttgcggagcc tgactactac accccgctaa aaccagaacc
     3121 gccaacaatg acagcgaaga tagatgattg acgcgcgaca ttacttctcc tttttagctc
     3181 aaggtatggg ttacttcctg aattttgtca ccatccagct catgttcaaa gctgcgcaga
     3241 cgtttgtaga tagacatcaa ctcaaccagt gtggtccatg agttaatcag gtactggaaa
     3301 gcaccgcgaa cctgaccaaa aacgttggta atctgcgtca tcaggccgag cgtaatcgta
     3361 ccggcaacaa ttgacggaaa cagcaagaac aaaccgaaaa cgttatcgac ctgcaaatag
     3421 agaatgcggg cgatgttgaa atacatatag tgaaaataga ggcgaaaata gtttttccgt
     3481 acggcgctaa acagctcgcg taccgtaggc ggcgtcgcgc gcgtggcatc gtcttcacca
     3541 taaaccagct ctttacggta ggcggcctct actcgctggt ttttaaactc cagccccggc
     3601 agtttgatcc ctaccactgc cagcaatccg gtccccatca gcgaccagac gatagcggca
     3661 atcaccagac catacggaat gtgcccgata atcggcagct ccggcacatg cgcggagagc
     3721 gttaccagca ccggcaggaa ggcgatcaac gtcatgatgg cgttgataaa actgacgccc
     3781 atattctcca gcgttgaagc aaaacgcatg gtgtcttcct gcacacgctg tgcggcacct
     3841 tcgatatgac gcagttgttg ccagttcgcc atgtaatatt cgttcatcgc cgtacgccag
     3901 cggaacacgt agtgactgac aaagaagttg ttcagtacac tgatcaccac cgcgatcagc
     3961 gcaatcccca gaaagacgcc cacttcgcgg taaaattgtt cgatagtgac tttatgcggc
     4021 gaacttagcg cggtttgaat cagatcatag aacggcgcat accaggcgtt gacggcgacc
     4081 ccgacttcca ccaaaaacca agtgacgaag ataatcagcg cagtaccgag tattgaccag
     4141 tattgccaac ggtgcgggct gtagataaac cagaaaaatg caaaaagtcc tacgcaaaca
     4201 atgtagtaag cgtaaaaaat caggaaatcc aacgaccaga aacgcgcggc gctaatcggg
     4261 atctgcccgg aagcgccggt gatacgtgcc acccagtcac ccccaccggc ttgccagaag
     4321 ataacggcaa tcaatgccca aacaaaggcc gagagaaaaa acgttcccgg ctttgggaaa
     4381 aaagacttaa acatatgcac tcctgctaac ttcttatcgt tttaacaact gctgtgtatt
     4441 aaccgcatga ccgctacaaa gcgtcagcca aatgaaaccg cccattgtga cccatagcaa
     4501 ggcgcttagt tcgccagcct gaccataaga attgtttcga cggatttcac aaaccaaaga
     4561 ttgtttacat aagcggatta acacgcgcag gatcacaaat gcaggcattg gtgagcagga
     4621 aaaagatcgt gtttacgcgc cgttacgttt ttaacactat tattgcacta attcgccctt
     4681 tgcaatccat caatgagtag tataaatacg ctcagttacc ttcattcaat ctatggacac
     4741 caccgttgaa acgtagtctg cttttttctg ccgtgctgtg tgcggcgtca ttgacctccg
     4801 tccatgcggc gcagccgatc actgaaccgg agtttgcctc tgatattgtc gatcgttatg
     4861 ccgatcatat tttttacggc agcggtgcca cggggatggc gctggttgtt atcgacggta
     4921 accagcgcgt ctttcgtagt tatggtgaaa cgcgacctgg taataacgtt cgcccgcagc
     4981 tggattcggt cgtgcgtatt gcttccctca ccaaactaat gaccagtgaa atgctggtga
     5041 aattgctcga ccaggggacc gtaaagttaa acgatccgct aagtaaatat gctccgccag
     5101 gcgcacgcgt gccgacctac aatgggacgc ccattaccct agtgaatctg gcaacccata
     5161 ccagcgccct gccccgtgaa cagcccggtg gcgcggcaca tcgtccggtg tttgtctggc
     5221 caacgcgcga gcaacgctgg aaataccttt ctacggcgaa gctgaaagcc gcgccaggca
     5281 gtcaggcggc gtactctaac cttgcgttcg acctgctggc agatgcactg gcgaatgcgt
     5341 ccggcaagcc ttatacccag ttgtttgaag agcagattac ccatccattg gggatgaaag
     5401 acaccaccta caccccctca ccggatcagt gccgccgttt aatggttgct gagcgcggtg
     5461 ccagtccgtg caataacacg ctggcggcaa ttggcagcgg cggcgtctat tccacgcctg
     5521 gcgatatgat gcgctggatg cagcagtatt tgtcatctga tttttatcag cgcagtaacc
     5581 aggcagatcg catgcagacg ttgatttatc agcgcgcgca gtttacgaaa gtgattggca
     5641 tggatgtccc cggcaaagcg gatgcgcttg gtttaggctg ggtatatatg gccccgaaag
     5701 agggtcgtcc gaggattatt cagaagacag gcggtggtgg cggtttcatt acctatatgg
     5761 cgatgatccc gcagaagaat attggcgcgt ttgtggtggt cactcgttcg ccgttgacgc
     5821 gctttaaaaa tatgagcgat ggcatcaatg atctggtgac cgagctaagc gggaataaac
     5881 cgttggttat ccccgcatcc tgattaatac tctgaaggca aacggttgat agcgaccagt
     5941 ttgcctttat tcatttcgat atagccgcct ttacgcaaag cagcgagaac ttctgcgacg
     6001 acagaacgcg atatgcgtgt acgttgatgg atataattca tcacaccaat acgtgatcgc
     6061 aattcttcat tccagtcaat cattgataat aatgttgcgc gggtttgttt ggctggtaga
     6121 tgatatcccg tacaatttcc ttctgatatt aatttgtatg gtatatcgtt tttcattaaa
     6181 ccatcagcca gccccataat ataaggagcc tgggtaatac cgataagtac gttttcttct
     6241 ctacgcagag agataactcc ttctaaaata acgaaagtat cttcgttatt gacatcactg
     6301 gaaaatataa cttgcttttc attattaaac tcgaagcgcg taccgtatct ggacaaacat
     6361 ttatcgagct taccaaattc ctgaagaggt ttaactacag ataacatttg cgcgtccttt
     6421 gcagtaatgc ccgtcaaatc cttgacgggc attatttaga ttaaattacc agtatttctt
     6481 cggagtgaag aatattacca agtatattta acacccacgt tcgcggacca gtcttgatct
     6541 acgtcaccac caccgaggta gttagcatcg gtataggcgc tgaagttctt ggtgaagctg
     6601 aactgagtac ccagaccaac acgtaccgca gacccttcag taccgttatc gatggaatca
     6661 ccgttcacat cgttatcgtt gttagagtcg tcgtagacgt aagccagttt gaagtacgga
     6721 gtcagagcct gatcttcgct gtaggtgaag gtataacctg catctacacc cagttcataa
     6781 cgcatgctgt cgtaaggctg accgtcaact ttcatgtcgt tgctcagctg gtagtcatca
     6841 ccagactgga acagaccaga aatgctgccg taaggagtca cgtaaccagc atcacccagt
     6901 ttgaagtcgt aaccggtttt caaaccgaag ccccaagcgt cggagttggt gctaccgtca
     6961 acgtaagtac cgttgctcat ggttgcagac aggtcgttgt tgaagtgaga gtagctcaag
     7021 ct
//
LOCUS       DAZSNO010000186         6922 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZSNO010000186 DAZSNO010000000
VERSION     DAZSNO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.188"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..694
                     /locus_tag="ACHWN2_003639"
     CDS             <1..694
                     /locus_tag="ACHWN2_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR8933006.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="ACHWN2_003640"
     CDS             complement(1033..1803)
                     /locus_tag="ACHWN2_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933007.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2334)
                     /locus_tag="ACHWN2_003641"
                     /pseudo
     CDS             complement(1848..2334)
                     /locus_tag="ACHWN2_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2294..2515)
                     /locus_tag="ACHWN2_003642"
     CDS             complement(2294..2515)
                     /locus_tag="ACHWN2_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933008.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="ACHWN2_003643"
     CDS             complement(2516..3199)
                     /locus_tag="ACHWN2_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR8933009.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="ACHWN2_003644"
     CDS             complement(3263..3574)
                     /locus_tag="ACHWN2_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933010.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="ACHWN2_003645"
     CDS             complement(3571..4473)
                     /locus_tag="ACHWN2_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR8933011.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5872
                     /locus_tag="ACHWN2_003646"
     CDS             4913..5872
                     /locus_tag="ACHWN2_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR8933012.1"
                     /translation="MLKVSCDDGSTNVKLAWFEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5872..6267
                     /locus_tag="ACHWN2_003647"
     CDS             5872..6267
                     /locus_tag="ACHWN2_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR8933013.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6450..6830
                     /locus_tag="ACHWN2_003648"
     CDS             6450..6830
                     /locus_tag="ACHWN2_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFR8933014.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1892 a         1790 c         1676 g         1564 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggtttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     5101 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     5161 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     5221 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     5281 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     5341 agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa aagataaagt
     5401 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5461 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5521 ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat gtaattattt
     5581 tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga cattaataaa
     5641 tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga aatctctggc
     5701 agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct atctcactgg
     5761 cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc tgaaagtgaa
     5821 caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc
     5881 agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact ttatccgcag
     5941 gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg agatttctac
     6001 agacaatccc ttatttgcgg agcagctctt tattctgttg ccccccgact gttaacgcta
     6061 atcagcgttt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact tatagaacaa
     6121 acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat catagaggca
     6181 tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga acaaaccaga
     6241 cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca attacctgct
     6301 cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat cataaaaaat
     6361 gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat
     6421 tattgtcagt gtgtgacaat aacagagaga tgacgatggc gaccattaac gcacgtattg
     6481 atgatgatat aaagaaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc
     6541 aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca
     6601 cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag
     6661 ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag
     6721 caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa
     6781 taggtcttct ttcagataac cgggatgctg aactaggctg tgtcccttaa tcatctgagt
     6841 tatagtaact gtctgtttac acaacatatt gaactatggc tcgctacgac cttcccgatg
     6901 aggcatggac catcatcaag cc
//
LOCUS       DAZSNO010000187         6864 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZSNO010000187 DAZSNO010000000
VERSION     DAZSNO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.189"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(269..754)
                     /gene="sixA"
                     /locus_tag="ACHWN2_003649"
     CDS             complement(269..754)
                     /gene="sixA"
                     /locus_tag="ACHWN2_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR8933015.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(957..3101)
                     /gene="fadJ"
                     /locus_tag="ACHWN2_003650"
     CDS             complement(957..3101)
                     /gene="fadJ"
                     /locus_tag="ACHWN2_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR8933016.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3101..4411)
                     /gene="fadI"
                     /locus_tag="ACHWN2_003651"
     CDS             complement(3101..4411)
                     /gene="fadI"
                     /locus_tag="ACHWN2_003651"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR8933017.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4591..4875)
                     /locus_tag="ACHWN2_003652"
     CDS             complement(4591..4875)
                     /locus_tag="ACHWN2_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR8933018.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5247..6587
                     /gene="fadL"
                     /locus_tag="ACHWN2_003653"
     CDS             5247..6587
                     /gene="fadL"
                     /locus_tag="ACHWN2_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR8933019.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1714 a         1833 c         1621 g         1696 t
ORIGIN      
        1 ttacatcctg cctgattatt caactctatt ttctctatta tctgtattaa taaatgacaa
       61 tgagtgtcaa acagatatta ttcatttcaa catgtaactt tgggcaaaat caagatacgc
      121 gtagaagaat aatttccgaa tgaatgtaga aagtgtttta tttatagata aggagagtct
      181 tcgatagtag tgaggttgac agatatccgc tgcctaatga gcaattatgc ccaatgaatc
      241 tacctcattg ggcataattt ggaacacatc agatagcttt tgccatcttc agattacacg
      301 ggctcatctg ccagttgaat gtaccgtttc cgctttcatc aagcgttacg ctggcaatgg
      361 ccgaggtggt aaacataggc ggcgtttcgc ccggacataa ctcggcaacc agatacccga
      421 ctaacggtaa gtgagagatc accagtaccg aagcaacacc ttcattggtc aacgcctgca
      481 aataggcact gaccagacca acatcgccgc agggcgttaa ctccggcaga acttctgcac
      541 tggaaggcag gttcagacaa tcccctactt cttccagtgt ttgctcggct cgcaggaacg
      601 ggctcaccag aacgcgttcg atttccactt tttgaccttt cagccagttc gccatcaggc
      661 gagattcgtc acaaccatta gtggtcagag gacgaaccga atcactggcg gaatcgaggg
      721 ctgcgtcgcc gtgacgcatg ataaaaactt gcatattgca ccgcttttgt taaccagatt
      781 cgcccgtttt ctttcatctg aaaccagaga aagaaaacgg tggccggcat tgtgccttat
      841 ccattcaccg aatgaaacgc tgttttttac ctcaatggcg taagtatagt caatatgcgt
      901 ttacattttg accaacactc cgccattcag cgcggattca tatagctttg accttcttat
      961 tgcaggtcag ttgcagttgt tttccaaaaa ctttccccac gcgcgcccat ctcgaccaaa
     1021 cgctcgcaag gggtaaaacg ggaaccatac tgcgtggcca gtcgttgcat tattgcaacc
     1081 acttcgcccg cgccgagaga atcgatatag cggaacggtc caccgagaaa tggcggaaaa
     1141 ccaatgccaa ataccgcacc aatatccccg tcacgcacgc tacggataac ctgctcatca
     1201 acacaacgta ctgcttcatt cagcatcaac atcacacacc gttcagcaac ctgcggtgcg
     1261 gagagtcgcc cctgcccttg tgcgccaatg agcgggtaaa tggcaggatc gacctgtttt
     1321 ttgcttttac gccctttctg accataaaga tagaaacccc gaccattttt tctgcctttg
     1381 cgatcgtcgt tcaaaattga agaaacaaca tttgcaggcg cgctaaaacg ttctccataa
     1441 gcggcttcca gtacaggaat aattttagtc ccggtgtcga ttcctacctc atccaaaagt
     1501 tggattgggc ctaccggaaa accaaatttc actagcgcgg catcaatgtg ctctacccgt
     1561 tcaccttcgg tcagcatgcg gatagcttca ttaatgtaag gcgctaagat gcgattgacg
     1621 taaaacccgg ctttgtcacg cacgacaatg ggcgttttgc cctgtttttt cgccagtttt
     1681 accgtagtgg cgatggtttg cgccgatgta cccgcatgag gaataatctc taccagcggc
     1741 attttttcca ccggactgaa gaaatgcagg ccaataactt gctcaggtcg cgcggcgtga
     1801 gcggcgatat caccaatcgg taaagatgac gtgttcgaag caaaaatggt atgagcggcg
     1861 caattttgct ccacttccgc caccatctgt tgttttaatt cgagattttc aaacaccgct
     1921 tcaataatca gatcgcgatg ggcaaagccg cgatagtccg tcgttccgga gattaatgcc
     1981 agctgtttgt cacgttcgct ggctttgaga tgacgacggc gaactttgcc ctccagctga
     2041 tcccaactgt acttcagcgc atgatttatg ccctgcgggt tgatatcttt aattctgacc
     2101 ggaagccccg ctttacaagc agtgacataa gcaataccgc cgcccatcaa gccaccacct
     2161 aaaattccca cgctgtttaa tggcgcaggc ggtgcatcac taccgggatc tttcttcacg
     2221 tccgtactgg caaaaaagat actacgcaaa gcttgcgatt gcggcgtcat cgccagttcg
     2281 ccaaaggcgc gagcttcagc gtcatagccg ctgctggtgc cctgcgctaa tcccgtttca
     2341 acaacctcca ggatgcgttc tgtcgccgga taattgcctt gagttttgtg ttctgttttc
     2401 ttgccgacca ttttgaacag cagcgcacga cctaacggcc ccgccagaat acgctcgcgt
     2461 acaggtagag ggcgggaaga tgggcgatcc ttcttcgcca gctcaacagc ggcttccagc
     2521 aaaatggagt gcggaacaac gtcatccacc agccccagct ttaatgcctg tttcgcccga
     2581 agttgttttc cggtgagaat catctctaat gctgtactga cgcctatcag acgcggtaaa
     2641 cgctgggtgc cgcctgaacc gggtaacaat ccaagttgta cttcaggcaa accgagcacc
     2701 gttttaggat cgtcagtaca aacgcgaccg tggcacgcca gcgccagctc cagcccaccg
     2761 cccaggcaag cgccatgaat agccgcgata accggaatgg gcaaagcatg aatctccgcc
     2821 atcaactgtt ggccctgccg cgccagtgct tccgcttctt gcgccgtttt gcagttgcca
     2881 atcatgttga tgtctgctcc ggcgataaaa ttgtccggtt tcgcggagat aaacaccacg
     2941 ccgcgcaact ctttgttttc acggagttgc ttaataatgg cgcgcacctg cgaggcaaat
     3001 tccgccttca gggtattcat tttctcatcc ggtacgtcga tggtgatgat agcaatgttg
     3061 tccagacgaa cattaagagt aaacgctgat gccatttcca ttattccgcc tccagaacca
     3121 ttgccgcgcc aagcccaccg gcagcacagg cggtaactaa accaaatcca ccgccgcgac
     3181 ggcgaagttc atgcaatgtc tgggtaatca tccgcgcgcc ggtcgccgcg aagggatgtc
     3241 cgtaagcaat cgaaccgcca agcacgttaa atttgctatc gtccacttcg ccagtggcat
     3301 gtgcacgccc cagtacttca cgagcaaaac gttcactacc cagcaactga atattcgcca
     3361 gtgtctgagc tgcaaaggct tcgtgcatat cgatcaatgt cagttcgctc atcgtcaaac
     3421 cggcacgttc cagcgccagc ggtgttgacc aggctggacc gagcaacatg tcctgccaga
     3481 catcaatcgc agtaaatgcg tagctgcgca gataccccag cggcaccagc cctaattctt
     3541 tcgcccggga ttcagtcatc aggatcaccg ctgccgcgcc atcggtcagc ggcgtactgt
     3601 ttgccgccgt taccgttccg tgtttgcgat caaacgccgg gcgcagcttt gcgtaatcgg
     3661 caagcgagga attaccgcga atattgttgt cttcgacaag cggttgttta taaggaggga
     3721 taaaggcagt catcacctct tctttgagtt ttccgtctga ccatgcctga gcggcacgct
     3781 gatgcgaacg gtgcgctaat gcatcttgct gttctcgggt gatgccgtag gttttcgcca
     3841 tttgttcggc ggtgtcgccc atccgcaagc cagtagaata ttctgctacc gcaggcggta
     3901 cgggcattaa gtcgcgcaaa cgcaggcgag agaagagttt caggcgctgg ctcatggttc
     3961 gagctttgtt gacatcaacc agcacgtacg ccagtttttt actgacgcca attggcaata
     4021 cggaagagga atctgcccca ccggcaatcc ccgctcggat agttcccgcc atcaggcttt
     4081 ctgcgacgtt tgcaaccgct tggaaactgg tagcgcaagc gcggctgacg ctgtaagcat
     4141 cggtatgcac attcattccg gtaccgagaa caatttcacg cgcaatgttg ggggcttcag
     4201 gcatttgtac gacctgacca aagaccagct gttcaatcac ttcagcgggg atctcgctgc
     4261 gtgccagcag ttcgcctacc accatcttcc ctaaatcaac cgtgggaatg ccatgaaaag
     4321 ccgtcgcctg gcgggcaaaa ggcgtacgta aaccgctaac aatggcgata cgatcgccct
     4381 ggcgggtaac cagtggtaaa acctgaccca taacactccc ctgtaaaaaa ataaataaag
     4441 tggtctgacc tgatcatagt cttaaccatt tttttacatt tagccaagcg gagaaaaagg
     4501 aaagtgggag ctatgacaca gtgaagacag aagaaagaaa agaaaacgcc cctgccagca
     4561 actgacaggg gcgtacgtac ccgagaggaa ttaacgcaga cccagctgga agatcagcat
     4621 ttcagcttcg caggcaaaag tgaaatcgat atccaggcgc acaccgtcag actcttcagt
     4681 gaaagtcggg gagattttgc acggttcgga ttccacgcta cgggattttt cagtcagcgc
     4741 cgccagcgtt tgttctgctt ctgcgcggtt tgcaaacacg cggctgtaag acgcggtgca
     4801 gtcggagttg tccataatgg tgccaacatc catacagcag caaaccgggg tttcatcagc
     4861 actacattta ctcatcgttg atttcctctg tatgtgcacc caaggtgcca gataaacgtt
     4921 gtggatattt tacgcttccg gaaagtgctg ctccagttgt taattctgca aaatcggata
     4981 agtgaccgaa atcacactta aaaatgatct aaaacaaaat tcacccgaat ccgcgagtgc
     5041 gcaacctcca aattttgcca gctggatcgc gtttcttaga tcatatttga aaaaagatgg
     5101 aaacatactt gcaacattct agctggtccg acctatactc tcgccactgg tctgatttct
     5161 aagatgtacc tcagacccta cacttcgcgc tcctgttaca gcacgtaaca tagtttgtat
     5221 aaaaataaat cattgaggtt atggtcatga gccagaaaac cctgtttaca aagtctgctc
     5281 tcgcagtcgc agtggcactt atctccaccc aggcctggtc ggcaggcttt cagttaaacg
     5341 aattttcttc ctctggcctg ggccgggctt attcagggga aggcgcaatt gccgatgatg
     5401 caggtaacgt tagccgtaac cccgcattga ttaccatgtt tgaccgcccg acattttctg
     5461 cgggtgcggt ttatattgac ccggatgtga atatcagcgg aacgtctccg tctggtcgta
     5521 gcctgaaagc cgataacatc gcgcctacgg catgggttcc gaacatgcac tttgttgcac
     5581 cgattaacga ccaatttggt tggggcgctt ctattacctc taactatggt ctggcaacag
     5641 agtttaatga tacttatgca ggcggctctg tcgggggtac aactgacctt gaaaccatga
     5701 acctgaactt aagcggtgcg tatcgcttaa ataatgcatg gagctttggt cttggtttca
     5761 acgccatcta cgctcgcgcg aaaattgaac gtttcgcagg cgatctgggg cagttggttg
     5821 ctggtcagat tatgcaatct cctgccgggc aaactcctca agggcaagca ttggcagcta
     5881 ccgccaacgg tatcgacagt aataccaaaa tcgctcatct gaacggtaac cagtggggct
     5941 ttggctggaa cgccggaatc ctgtatgaac tggataaaaa taaccgctat gcactgacct
     6001 accgttctga agtgaaaatt gacttcaaag gtaactacag cagcgatctt aatcgtgcgt
     6061 ttaataacta cggtttgcca attcctaccg cgacaggtgg cgcaacgcaa tcgggttatc
     6121 tgacgctgaa cctgcctgaa atgtgggaag tgtcaggtta taaccgtgtt gatccacagt
     6181 gggcgattca ctatagcctg gcttacacca gctggagtca gttccagcag ctgaaagcga
     6241 cctcaaccag tggcgacacg ctgttccaga aacatgaagg ctttaaagat gcttaccgca
     6301 tcgcgttggg taccacttat tactacgatg ataactggac cttccgtacc ggtatcgcct
     6361 ttgatgacag cccagttccg gctcagaatc gttctatctc cattccggac caggaccgtt
     6421 tctggctgag tgcaggtacg acttacgcat ttaataaaga tgcttcagtc gacgttggtg
     6481 tttcttatat gcacggtcag agcgtgaaaa ttaacgaagg cccataccag ttcgagtctg
     6541 aaggtaaagc ctggctgttc ggtactaact ttaactacgc gttctgataa cgcgttcgcc
     6601 tggataaagt cacctgctat gcaggtgact ttaactcccc cacttcaccg aaagtagttt
     6661 cctcattgct tgcctcctga gttttgcaaa cagcctgatg gcagcttgcc ctttcaaatc
     6721 agtaagtggt atccacagac aaacctgaag ggaaatgcat attttcaagc gttctgctcg
     6781 tccttctcaa agagtttact tttctgcatt tccaggatac tcaacccccc tggctattgt
     6841 gcgcttatac actcaaatta aaga
//
LOCUS       DAZSNO010000188         6848 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZSNO010000188 DAZSNO010000000
VERSION     DAZSNO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.190"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..236)
                     /locus_tag="ACHWN2_003654"
     CDS             complement(<1..236)
                     /locus_tag="ACHWN2_003654"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933020.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEG"
     gene            complement(325..1203)
                     /gene="narH"
                     /locus_tag="ACHWN2_003655"
                     /pseudo
     CDS             complement(325..1203)
                     /gene="narH"
                     /locus_tag="ACHWN2_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
     gene            complement(1200..4940)
                     /gene="narZ"
                     /locus_tag="ACHWN2_003656"
                     /pseudo
     CDS             complement(1200..4940)
                     /gene="narZ"
                     /locus_tag="ACHWN2_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            complement(5022..6410)
                     /gene="narU"
                     /locus_tag="ACHWN2_003657"
     CDS             complement(5022..6410)
                     /gene="narU"
                     /locus_tag="ACHWN2_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR8933021.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            complement(6734..>6848)
                     /locus_tag="ACHWN2_003658"
     CDS             complement(6734..>6848)
                     /locus_tag="ACHWN2_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933022.1"
                     /translation="LDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
BASE COUNT         1651 a         1774 c         1784 g         1639 t
ORIGIN      
        1 ccttcgtcat caacacgtac aaaccagaag accagctttt tgtttcccgc atccacaaag
       61 aacggaatat tcaggtctgc gcagcattca acggcatcgt caaaactatc aaagcgcaga
      121 acttctgcgt cttcttcgtc aaaaaaatca tcttcgtgaa gcttcacgac atagcgggga
      181 agtttgcttc tttgagaggc gggtttacgt ttacggggtt tagctgaacg ggccatataa
      241 ccacctgaaa gacaatgaca ttgcctgttt ttataacggt aattgcagac catgacaagc
      301 cgcagccgtc aggctgccta ctcgatagag gtcaacttcg cgctcggtgc tcgacgcttc
      361 ctcgatgcgg tcggcgtcgt aaagcagcac gcccagatac cggatgcgac ccacgcaggt
      421 ttctgagcac acggtgggtt ggcctgactc aattcgcgga taacagaaga tacatttttc
      481 tgacttgccg cttttccagt tgaagtagat ttttttgtac ggacaaccgc taatgcacaa
      541 acgccagcca cggcatttat cctgatcaat cagcacaatg ccgtcttctt cgcgtttgta
      601 gatagcgccg cttgggcagg tcgccacgca gctgggattg aggcagtgtt cgcacaggcg
      661 cggcaggtac atcatgaagg tgttttcaaa ctgcccgtac atctcctttt gcatggcctc
      721 gaagttgcgg tcgcgggcac gtttttcaaa ctcgccgccc agcagttctt cccagtttgg
      781 gccccagatc actttgtcca tccgtttgcc gtcaatcagt gaacgcgggc gagcagtagg
      841 gatatgtttg ccttccggtg tgctatgcaa atgttcgtag tcgaaggtga aaggttcgta
      901 gtaatcatca atctgctgcg gcaccaccgg gttggcgaag attttggtta ttacgcccat
      961 cttgctgccc agacgcggac ggatcttgcc attcacatcg cggacccagc cgccttgcca
     1021 ctcttcctga tcttcccagt ttttcggata accaatgccc ggtttggttt cgacgttgtt
     1081 aaaccatgcg tactccatgc cttcgcgtcc ggtccagacg ttgttacagg tcaccgaaca
     1141 ggtatggcag ccgatacatt tatcgaggtt aagcaccatg ccgacttgcg aacgtatttt
     1201 cattttttcg cctcctgtac ctgatcgcga ccttcatcat ccagccagtt aacgttcttc
     1261 atcttgcgga tcatgatgaa ttcgtcgcgg ttcgaaccga cggtgccgta gtagttaaag
     1321 ccccaggcca gctgcgcgta accgccaatc atatgcgtcg gtttcgggca aacgcgggtg
     1381 accgagttat gaatgccgcc gcgcatgcca gttacttccg aaccaggaat attcataatg
     1441 cgttcctggg cgtgatacat catggtcatg cccggcggta cacgttggct gaccaccgcg
     1501 cgggcagtca gcgcgccgtt ggcgttaaat acttccaccc agtcgttatc gacaatggtc
     1561 agttctcggg catctgtttc gctgatccag acaatcggtc caccgcgaga gagcgtcagc
     1621 attagcaggt tttcactgta ggttgagtga atgccccatt tctggtgcgg cgtcaggaag
     1681 ttaagtgctt tttcctggaa gccgtttggc ggtatctggc gcatctcact gacgctacgg
     1741 gtgtcgatag gcgggcgata agccaccagg ctttcaccaa aagcacgcat ccacggatga
     1801 tcctgataga gctgctggcg tcccgacagc gtgcgccacg gaattaactc atgaacgttg
     1861 gtgtatcccg cgttatagga tacgtgatcg ctttccagac cggaccatgt ggggctggag
     1921 ataattttac gcggctgcgc ctgaatatcg cgaaagcgaa tcttctcgtc ctctttgtgc
     1981 agcgccagat gggtatgttc gcgcccggtg atctcgccca gcgcctgcca cgctttgact
     2041 gcaacatgac cgttggtttc cggtgccagt gccagaatca cttctgaagc gtcaatggcg
     2101 gtgtcaatca gcggacgccc ctgtgccggg ccatcacgct tggtgtaatt gagtttgccg
     2161 aggaaatcga tttcatcctg ggtattccac gaaatccctt taccgccatt gccaagtttg
     2221 tccatcaatg gcccgagcga ggtaaagcgt tcatacgtag cagggtagtc gcgctccacc
     2281 gccacaatat tcggcgcggt tttacccggg atcagatcgc attcgccttt gcgccagtcg
     2341 agcacttcac acggctgtga gagctctgcc ggagagtcat gcagcagtgg ttgtaatacc
     2401 acgtcggttt ctttgccaag atggcccacg cacacttgcg aaaatgcttt ggcaatacct
     2461 ttgtagattt cccagtcgct gcgtgattcc caggccggat cgaccgccgc agaaagcgga
     2521 tgaataaatg gatgcatatc cgaggtgttc atatcgtctt tttcatacca ggtggcggtg
     2581 ggcagaacga tatcggagaa caggcaggta ctggacatgc ggaagtcgag cgtcaccagc
     2641 aggtcgagct tgccttcaat cgccgcagtt tgccactcga cttcttccgg tttgatcccg
     2701 tcgctggcac cgagttcctc gccctgaatc ccgctttcgg tccccagcag atacttctgt
     2761 atatactcgt ggcctttgcc ggaggagcca agcaggttag agcgccagac aaacaaatta
     2821 cgcggatgat tgctgccgct atctggttgt tcgcaggcca tacgtaaatc gcccgatttc
     2881 agcgcctggg cggtaaattc tgtgggggat aatccggcct tgtcggcttc agctttaatc
     2941 ccgagcgggt tacgccccag ctgcggcgca gaaggtagcc agcccatacg ttcggcgcga
     3001 acgttgaaat caatcagatg accgctgtac ttactggcat cggcgagcgg tgaaagtaac
     3061 tcctgagcag agactttttc atagcgccat tggctggaat gattgtagaa aaacgaggtg
     3121 ctgttcatct ggcgcggtgg tcggttccag tcgagcgcaa aggccagtgg caaccagccg
     3181 gtttgtgggc gcagtttttc ctggccgaca tagtgtgccc agccgccacc gctttgcccg
     3241 acacaaccgc agaagatcag catattgatc atcccgcggt agttcatgtc catgtgatac
     3301 cagtggttaa cacctgcgcc gaggataatc attgagcgcc catgcgtttt atgggcggta
     3361 tcggcaaatt cgcgggcgat ggtttcaata tactggcgcg gtacgccggt aatttgctca
     3421 ccccaggctg gggtgtacgg tttgatttca gcgtaatctt tcgcgctgtt ttcatcttcc
     3481 agcccgcgat cgaggccgta attcgccagt accaaatcat aaacgctgac caccggacag
     3541 gtgttgccat cgactaacgt caggttttta acgggcaatt ggcgcaccag caccggattg
     3601 tgttttacgc tgcgaaaatg cggattttca atgccgccaa agtaggggaa ggccacgcct
     3661 gcaacagcgt catgttgacc gagcagggtt aacgacaatt cggtttccgt accggcggca
     3721 atggattcca gattccattt gcctttttct ccccagcgga aaccaatcga accgttcggc
     3781 actaccaatt cacctgcggt attaactgct acggttttcc actgcggatt gttgctttcg
     3841 cccagtccat ccaccaggtc agatgcgcgg atcatgcgcc cgggaacgta gctaccatcg
     3901 tcgcgaggct ccagcattac cagcatcggc atgtcgctgt agcggcggca gtagttgata
     3961 aagtagtcgc tgggattatc gagatgaaac tcttttaaaa tcacatgccc cattgccatc
     4021 gccagggcgc tatcagtgcc ttgtttcggt gccagccact ggtcgcacaa tttggccact
     4081 tcagagtagt caggggtaat ggcgatggtt ttagtgcctt tgtagcgtac ttcggtaaag
     4141 aagtgggcgt ccggcgtacg tgtctgcggt acgttagacc cccaagcgat gatgtagctg
     4201 gagttatacc agtcggcaga ttccggtacg tcggtttgct cgccccaggt catcggcgag
     4261 gcgggcggca ggtcgcaata ccagtcgtag aaactttaac aggtgccgcc aagcagcgac
     4321 agataacgcg ttccggcggc gtaagaaacc atcgacatcg ccgggatcgg cgagaaaccg
     4381 gcaacgcgat ccgggccgta ggttttgatg gtccagacgt tagcggcggc aatcagctgg
     4441 tttagttcct gccagttgga gcggataaac ccgccgcgcc cacgcacttg tttgtagctc
     4501 aggcactttt gcggatcatt cataatcgat gcccacgcca gtaccggatc gctgtgttgt
     4561 ttgagggctt cgcgccacag ttcaatcagt cgtttacgaa tgagcgggta tttcaggcgg
     4621 ttagcgctgt aaagatacca ggagtaactt gcgccacgcg ggcagccgcg aggttcatga
     4681 ttgggcaggt cagggcgagt gcgcgggtag tcggtctgtt ggatttccca ggtcaccaga
     4741 ccatttttaa cgtagatttt ccagctacag gagcctgtac agttaacgcc gtgggtggaa
     4801 cgcacgattt tgtcgaactg ccaacgctgg cgatagctgt cctcccagtc gcggttgcta
     4861 tgcatcacct gtccgtgacc atcggcaaag gtttcgccct tttgtttgaa gtagcgaaag
     4921 cgatccaaaa gtttactcat gacatgactc ctgctccagg aatgaattgg gatctgatgc
     4981 cttgtcggat gcgacatggt tgtacatcag gcatcgatct cttatttttg gctgaacttc
     5041 cgccgaccat aaaccagcca ggtcagcagc acacaaacga tgtagaagat taaaaacact
     5101 ttcatcgcgc cgaccggaga gccggtcata ttgagcgaca tgccaaatgc ctgcggaata
     5161 aaaaagccgc ccactgcgcc aatggctgag ataaagccca gcgttgccgc cgtttcggtg
     5221 acggcttctc tctgagcttg ctcatcacta ccgcctttca tcttcacccg ataaatggtt
     5281 atctggcgaa agatgacggc gatcatctgg aaagtagaac cacttcccag acccgcggtc
     5341 agaaacagcc ccataaatac ggcgtaaaag gcgatgaaat taccggagcc tgtgcccggt
     5401 aaggtaagga acagcagggc actgaaaatc gccataaaaa taaagttgat caacgtcacc
     5461 cgcacgccgc cgaatttatc ggaaatagca ccaccaaccg accgcgcgat ggcaccaata
     5521 aatgggccaa agaacgccag gcgcagaata ttcacatccg ggaactgggt ttttgccagc
     5581 atggcaaaac ccgcagaaaa accgataaac gaaccgaagg tggcaaggta aagcaggctc
     5641 agcagccaga gatgcaggcg ttgtaagaca gggagctggt cggcaattga ggcgcgtgaa
     5701 ctggcgatat cattcatccc tgaccaggcg gcgatcgtgg caatcgccag tagcggtacc
     5761 caaatccatg cggcattcgc cagcgacatc accgaaccgt cggcctgcgg tacgccattg
     5821 acgccgagaa aggcaaatac aggtacaaaa atgaccagcg gtgcaaccag ctgcattaca
     5881 cttacaccta agtttcctaa tccgccatta atcccaagag cgctcccttg tttggctttt
     5941 ggaaagaaga aactgatatt gcccatgctc gaagcaaagt ttgcacctgc aaaaccgcat
     6001 agcaaagcga taacgataaa tatcccaaaa ggagtattcg gattttgcac ggcaattccg
     6061 agccagacgc aaggaataat caggattgca gtactaaaaa ccggccatcg gcgtccaccg
     6121 aatataggca ccataaagga gtagggaaca cgcaataatg cgccagaaac ggagggtaat
     6181 gcggttaata aaaagagttg atcggtagta aaattaaaac cgattttatt gagattaacg
     6241 gtaactgcgc taaatagcat ccagacacag aaggcaagaa gtagacaact gactgatatc
     6301 cagaggtttc ttcgagcaat atgttttcct ttattttccc agaaggccgg attttctggt
     6361 ttccagtcgc gtaaaagata acgactattt ttctcatttt gcagtgccat attgttcctc
     6421 atatgcacac attggtaatg aaaaaagaca aaacaggagg taaggcgcaa tagccagtta
     6481 ttagaattaa ggatgaattg ggtgaagtgc tgattgaaag aatagataag aaagcgtaac
     6541 cgcgggggca gaatggggat taagtagcca gatatgtgtt actaaatgta actaacaagc
     6601 taaccttcaa acgggggtta atctttgaac gtatgtcatg tttcaaccct tcagatcgtg
     6661 aatctaaagg gttacatatt aactatattc tcaacaagac cagacactta tagaattagt
     6721 gatgatttat tcactaaaaa ggccatgaat tggaggaaaa cgatctgtcc agaaagtcta
     6781 tcggttcctc tcttattgcc tgattgtaat aatcgttatt atagtaagta ttgtttattt
     6841 catctaag
//
LOCUS       DAZSNO010000189         6824 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZSNO010000189 DAZSNO010000000
VERSION     DAZSNO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.191"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..759
                     /gene="yneK"
                     /locus_tag="ACHWN2_003659"
     CDS             <1..759
                     /gene="yneK"
                     /locus_tag="ACHWN2_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR8933023.1"
                     /translation="HDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQFPMI
                     NLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHR
                     WFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQESPH
                     FQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETP
                     PKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            909..2099
                     /gene="ydeA"
                     /locus_tag="ACHWN2_003660"
     CDS             909..2099
                     /gene="ydeA"
                     /locus_tag="ACHWN2_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR8933024.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2124..2789)
                     /gene="marC"
                     /locus_tag="ACHWN2_003661"
     CDS             complement(2124..2789)
                     /gene="marC"
                     /locus_tag="ACHWN2_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR8933025.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3001..3435
                     /gene="marR"
                     /locus_tag="ACHWN2_003662"
     CDS             3001..3435
                     /gene="marR"
                     /locus_tag="ACHWN2_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR8933026.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3455..3838
                     /gene="marA"
                     /locus_tag="ACHWN2_003663"
     CDS             3455..3838
                     /gene="marA"
                     /locus_tag="ACHWN2_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR8933027.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3870..4088
                     /gene="marB"
                     /locus_tag="ACHWN2_003664"
     CDS             3870..4088
                     /gene="marB"
                     /locus_tag="ACHWN2_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR8933028.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4119..5018)
                     /gene="eamA"
                     /locus_tag="ACHWN2_003665"
     CDS             complement(4119..5018)
                     /gene="eamA"
                     /locus_tag="ACHWN2_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFR8933029.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5285..6400
                     /gene="ydeE"
                     /locus_tag="ACHWN2_003666"
                     /pseudo
     CDS             5285..6400
                     /gene="ydeE"
                     /locus_tag="ACHWN2_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            6527..6622
                     /gene="mgtS"
                     /locus_tag="ACHWN2_003667"
     CDS             6527..6622
                     /gene="mgtS"
                     /locus_tag="ACHWN2_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFR8933030.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            6622..6725
                     /gene="mgtT"
                     /locus_tag="ACHWN2_003668"
                     /pseudo
     CDS             6622..6725
                     /gene="mgtT"
                     /locus_tag="ACHWN2_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
BASE COUNT         1750 a         1680 c         1576 g         1818 t
ORIGIN      
        1 catgatggag aaattaatat tttttgcgct cccaacccaa agcctgtcac tatggggcgc
       61 gtatttcaga ccggcgtcga gcgtgtgctc tttctgttta tgaatgattt tattgaacaa
      121 tttccaatga tcaaccttgg cgcccccatc aaaagagcgc atacaccaca tattgaaccc
      181 ctgcctccgg atcaccatac cgctgcagat tacttacgcc agtttgattt gcttgtcctg
      241 aattttatct ctcgcggtaa ttttgtcata ctcccccgat tatggaataa ctctgaggtt
      301 cacagatggt tcgtcaataa agatcctaac ttgatcaccg ccattctcga cataacggac
      361 agcgaattaa aagaggattt attgcaaagc ctgatggatt cattaggttc taacaaacat
      421 gtacaacccg aagtctgcat ctgcttctta tcccttttag cagaacaaga atctcctcat
      481 tttcaggact tgtttttatt tttcgccaat atgttattgc actatcacca atttatgaat
      541 cccaatgaaa gtgatctgaa tgacgtgttg atgccagcat cattaagtga tgataaaatt
      601 atcaaacata tggcgcgcag gaccctcaaa ctgtttgtaa aaaatgaaac gccgccaaaa
      661 gttactcacg aagacctggt gaaaaacagg cctcgctccc ctgtcagacc acctataccc
      721 gcaaccgcca aaacgccaga cctccctgaa cgtcattaaa ccgtgatgtt accgactctc
      781 tgactcggaa cagaatcagc gtcagagaaa cggaaaacgc gatccagatc acaaatgcat
      841 tgtattcaca tcattaaccg ttttaagatc atttcatcac tttttcgcaa ctcacccgat
      901 aatctgttat gacaacaaac actgtttccc gcaaagtggc gtggctacgg gtcgttacgc
      961 tggcagtcgc cgccttcatc ttcaacacca ccgaatttgt ccctgttggc ctgctctctg
     1021 acattgcgca aagttttcac atgcaaaccg ctcaggtcgg catcatgttg accatttacg
     1081 catgggtagt agcgctaatg tcattgcctt ttatgttaat gaccagtcag gttgaacggc
     1141 gcaaattact gatctgcctg tttgtggtgt ttattgccag ccacgtactg tcgtttttat
     1201 cgtggagctt taccgttctg gtgatcagtc gcattggtgt ggcttttgca catgcgattt
     1261 tctggtcgat tacggcgtct ctggcgatcc gtatggctcc ggccgggaag cgggcacagg
     1321 ccttgagttt aattgccacc ggtacagcac tggcgatggt cttaggttta ccgctcgggc
     1381 gcattgtggg gcagtatttc ggctggcgaa tgaccttctt cgcgattggt attggggcgc
     1441 ttgttaccct tttgtgcctg attaagttac ttcccttact gcctagtgag cattccggtt
     1501 cattgaaaag cctcccgcta ttgttccgcc gcccggcatt gatgagcatt tatttgttaa
     1561 ctgtggtggt tgtcaccgcc cattacacgg catacagcta tatcgagcct tttgtgcaaa
     1621 acattgcggg attcagcgcc aactttgcca cggcattact gttattactc ggtggtgcgg
     1681 gcattattgg cagcgtgatt ttcggtaaac tgggtaatca atatgcgtct gcgttggtaa
     1741 gtacggcgat tgcgctgttg ctggtgtgtc tggcactgct gctacctgcg gcgaacagtg
     1801 aaatacacct cggggtgctg agtattttct gggggatcgc gatgatgatc atcgggcttg
     1861 gtatgcaggt taaagtgctg gcgctggcac cagatgctac cgacgtcgcg atggcgctat
     1921 tctccggcat atttaatatt ggaatcgggg cgggtgcgtt ggtaggtaat caggtgagtt
     1981 tgcactggtc aatgtcgatg attggttatg tgggcgcggt gcctgctttt gctgcgttaa
     2041 tatggtcaat cattatattt cgccgctggc cagtgacact cgaagaacag acgcaatagt
     2101 tgaaaggccc attcgggcct tttttaatgg tacgttttaa tgatttccag gatgccgtta
     2161 ataataaact gcacacccat acataccagc aggaacccca tcaggcggga gatcgcttca
     2221 atgccaccct tgcccaccag ccgcataatt gcgccggagc tgcgtaggct tccccacaaa
     2281 ataaccgcca ccaggaaaaa gatcagcggc ggcgcaacca tcagtaccca atcagcgaag
     2341 gttgaactct gacgcactgt ggacgccgag ctaataatca tcgctatggt tcccggaccg
     2401 gcggtacttg gcattgccag tggtacaaag gcaatattgg cactgggttc atcttccagc
     2461 tcttccgact tgcttttcgc ctccggtgaa tcaatcgctt tctgttgcgg aaaaagcatc
     2521 cgaaaaccga taaacgcgac gattaagccg cctgcaattc gcagaccggg aatcgaaatg
     2581 ccaaatgtat ccatcaccag ttgcccagcg taatacgcca ccatcatgat ggcaaatacg
     2641 tacaccgagg ccatcaacga ctgacgatta cgttcggcac tgttcatgtt gcctgccagg
     2701 ccaagaaata gcgcgacagt tgttaatggg ttagctaacg gcagcaacac caccagcccc
     2761 aggccaattg ctttaaacaa atctaacatt ggtggttgtt atcctgtgta tctgggttat
     2821 cagcgaaaag tataaggggt aaacaaggat aaagtgtcac tctttagcta accttgcatc
     2881 gcattgacca aaacttgaac cgatttagca aaacgtggca tcggtcaatt cattcatttg
     2941 acttatactt gcctgggcaa tattaccccc tacaactaat tacttgccaa ggcaactaat
     3001 gtgaaaagta ccagcgatct gttcaatgaa attattccat tgggtcgctt aatccatatg
     3061 gttaatcaga agaaagatcg tctgcttaac gagtatctgt ctccgctgga tattaccgcg
     3121 gcacagttta aggtgctctg ctctatccgc tgcgcggcgt gtattactcc ggttgaactg
     3181 aaaaaagtgt tgtcggtcga cctgggagca ctgacccgta tgctggatcg cctggtctgt
     3241 aaaggctggg ttgaaaggtt gccgaacccg aatgataagc gcggcgtact ggtaaaactt
     3301 accaccagcg gcgcggcaat atgcgaacaa tgccatcaat tagttggcca ggacctgcat
     3361 caagaattaa caaaaaacct gacggcggac gaagttgcaa cacttgagca tttgcttaag
     3421 aaagtcctgc cgtaaacaaa aaagaggtat gacgatgtcc agacgcaata ctgacgctat
     3481 taccattcat agcattttgg actggatcga ggacaacctg gaatcgccac tgtcactgga
     3541 gaaagtgtca gagcgttcgg gttactccaa atggcacctg caacggatgt ttaaaaaaga
     3601 aaccggtcat tcattaggcc aatacatccg cagccgtaag atgacggaaa tcgcgcaaaa
     3661 gctgaaggaa agtaacgagc cgatactcta tctggcagaa cgatatggct tcgagtcgca
     3721 acaaactctg acccgaacct tcaaaaatta ctttgatgtt ccgccgcata aataccggat
     3781 gaccaatatg cagggcgaat cgcgcttttt acatccatta aatcattaca acagctagtt
     3841 gataacgtga caacgtcact gaggcaatca tgaaaccact ttcatccgca atagcagctg
     3901 cgcttattct cttttccgcg cagagcgttg cggaacaaac cacgcagcca gttgttactt
     3961 cttgtgccaa tgtcgtggtt gttcccccat cgcaggaaca accaccgttt gatttaaatc
     4021 acatgggtac tggcagtgat aagtcggatg cgctcggcgt gccctattat aatcaacacg
     4081 ctatgtagtt tgttctggcc ccgacatctc ggggcttatt aacttcccac ctttaccgct
     4141 ttacgccacc gcaagccaaa tacattgata tacagcccgg tcataatgag caccgcacct
     4201 aaaaattgca gacccgttaa gcgttcatcc aacaatagtg ccgcacttgc cagtccgact
     4261 acgggcacca gtaacgataa cggtgcaacc cgccaggttt catagcgtcc cagtaacgtc
     4321 ccccagatcc cataaccaac aattgtcgcc acaaacgcca gatacatcag cgacaagatg
     4381 gtggtcatat caatagtaac cagactgtga atcatggatg cggaaccatc gagaatcagc
     4441 gagacaacaa agaagggaat gattgggatt aaagcgctcc agattaccag cgacatcacc
     4501 gccggacgcg ttgagtgcga catgatcttt ttattgaaga tgttgccaca cgcccaacta
     4561 aatgctgcag ccagggtcaa cataaagccg agcatcgcca catgctgacc gttcagacta
     4621 tcttcgatta acaccagtac gccaaaaatc gctaaggcga tccccgccaa ttgtttgcca
     4681 tgcagtcgct ccccgaaagt aaacgcgcca agcacgatag taaaaaacgc ctgtgcctgt
     4741 aacaccagcg aagccagtcc agcaggcata ccaaagttaa tggcgcagaa cagaaaggca
     4801 aactgcgcaa aactgatggt taatccatac cccaagagca aattcagtgg tactttcggt
     4861 cgtgcaacaa aaaagatggc cggaaaagcg accagcataa agcgcaaacc ggccagcatc
     4921 agcggtggca tgttatgaag ccccactttg atgaccacaa aatttagccc ccatacgacc
     4981 actaccagta gcgccaacac cccatctttt cgcgacattc taccgcctct gaatttcatc
     5041 ttttgtaagc aatcaactta gctgaattta cttttcttta acagttgatt cgttagtcgc
     5101 cggttacgac ggcattaatg cgcaaataag tcgctatact tcggattttt gccatgcgtc
     5161 cgatggttaa taacaacgac gaggcaagaa gggcgctggt agagcgtcgt agggataagt
     5221 tcatttgtct gtccggcagt gcgtttcgtt atgttttatt ttagagatgt aaagaaatag
     5281 catggctacg ctgccattta tgaccattta cttgagtcgc cagtacagcc tgagtgtcga
     5341 tctaatcggt tatgcgatga caattgcgct cactattggc gtcgttttta gcctcggttt
     5401 tggtatcctg gcggataagt tcgacaagaa acgctatatg ttactggcaa ttaccgcctt
     5461 cgccagcggt tttattgcca ttcctttagt gaataacgtg acgctggttg tgctcttttt
     5521 tgccctcatt aactgcgcct attctgtttt tgctaccgtg ctgaaagcct ggtttgccga
     5581 caatctttcg tccaccagca aaacgaaaat cttctcaatc aactacacca tgctaaacat
     5641 tggctggacc atcggtccgc cgctcggcac gctgttggtg atgcagagca tcaatctgcc
     5701 cttttggctg gcagctatct gttccgcgtt tcccatgctt ttcattcaaa tatgggtaaa
     5761 gcgcagcgag aaaatcatcg ccacggaaac aggcagtgtc tggtcgccga aagttttatt
     5821 acaagataaa gcactgttgt ggtttacctg ctctggtttt ctggcttctt ttgtaagcgg
     5881 cgcatttgct tcatgcattt cacaatatgt gatggtgatt gctgatgggg attttgccga
     5941 aaaggtggtc gcggttgttc ttccggtgaa tgctgccatg gtggttacgt tgcaatattc
     6001 cgtgggccgc cgacttaacc cagctaacat ccgcgcgctg atgacagcag gcaccctctg
     6061 tttcgtcatc ggactggtcg gttttatttt ttccggcaac aacctgctat tgtggggtat
     6121 gtcagctgcg gtatttactg tcggtgaaat catttatgcg ccgggcgagt atatgttgat
     6181 tgaccatatt gcgccgccag gaatgaaagc cagctatttt tccgcccagt ctttaggctg
     6241 gcttggtgcc gcgattaacc cattagtgag tggcgtagtg ctaaccagcc tgccgccttc
     6301 ctcgctgttt gtcatcttag cgttggtgat cattgctgcg tgggtgctga tgttaaaagg
     6361 gattcgtgca agaccgtggg ggcagcccgc gctttgttga tttaagtcga acacaataaa
     6421 aatttaattc agccttcgtt taggttatct ctgctaatat ctttctcatt gagatgaaaa
     6481 ttaaggtaag cgaggaaaca caccacacca taaacggagg caaataatgc tgggtaatat
     6541 gaatgttttt atggccgtac tgggaataat tttattttct ggttttctgg ccgcgtattt
     6601 cagccacaaa tgggatgact aatgaacgga gataatccct cacctaaccg gccccttgtt
     6661 acagttgtgt acaaggggcc tgatttttat gacggcgaaa aaaaccgcca gtaaaccggc
     6721 ggtgaatgct tgcatggata gatttgtgtt ttgcttttac gctaacaggc attttcctgc
     6781 actgataacg gatcgttgac acagtagcat cagttttctc aatg
//
LOCUS       DAZSNO010000190         6654 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZSNO010000190 DAZSNO010000000
VERSION     DAZSNO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.192"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="ACHWN2_003669"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="ACHWN2_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR8933031.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1102..1185
                     /gene="yqiM"
                     /locus_tag="ACHWN2_003670"
     CDS             1102..1185
                     /gene="yqiM"
                     /locus_tag="ACHWN2_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR8933032.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1207..1869
                     /gene="yqjA"
                     /locus_tag="ACHWN2_003671"
     CDS             1207..1869
                     /gene="yqjA"
                     /locus_tag="ACHWN2_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR8933033.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="ACHWN2_003672"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="ACHWN2_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR8933034.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /gene="yqjC"
                     /locus_tag="ACHWN2_003673"
                     /pseudo
     CDS             2403..2757
                     /gene="yqjC"
                     /locus_tag="ACHWN2_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2795..3100
                     /locus_tag="ACHWN2_003674"
     CDS             2795..3100
                     /locus_tag="ACHWN2_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR8933035.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="ACHWN2_003675"
     CDS             3103..3507
                     /locus_tag="ACHWN2_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR8933036.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="ACHWN2_003676"
     CDS             3497..3796
                     /locus_tag="ACHWN2_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR8933037.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="ACHWN2_003677"
     CDS             3982..4278
                     /locus_tag="ACHWN2_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR8933038.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="ACHWN2_003678"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="ACHWN2_003678"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFR8933039.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="ACHWN2_003679"
     CDS             5627..5995
                     /locus_tag="ACHWN2_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR8933040.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..>6654)
                     /gene="yhaJ"
                     /locus_tag="ACHWN2_003680"
     CDS             complement(6202..>6654)
                     /gene="yhaJ"
                     /locus_tag="ACHWN2_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFR8933041.1"
                     /translation="VIAPDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSEV
                     TRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMVE
                     KDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFAGK"
BASE COUNT         1677 a         1641 c         1669 g         1667 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taac
//
LOCUS       DAZSNO010000191         6484 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZSNO010000191 DAZSNO010000000
VERSION     DAZSNO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.193"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            157..504
                     /locus_tag="ACHWN2_003681"
                     /pseudo
     CDS             157..504
                     /locus_tag="ACHWN2_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            557..952
                     /locus_tag="ACHWN2_003682"
     CDS             557..952
                     /locus_tag="ACHWN2_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR8933042.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="ACHWN2_003683"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="ACHWN2_003683"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR8933043.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHWN2_003684"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHWN2_003684"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR8933044.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHWN2_003685"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHWN2_003685"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR8933045.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="ACHWN2_003686"
     CDS             complement(5323..6423)
                     /locus_tag="ACHWN2_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR8933046.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
BASE COUNT         1476 a         1701 c         1544 g         1763 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaat
//
LOCUS       DAZSNO010000192         6344 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZSNO010000192 DAZSNO010000000
VERSION     DAZSNO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.194"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHWN2_003687"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHWN2_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR8933047.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHWN2_003688"
     CDS             2219..2767
                     /locus_tag="ACHWN2_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR8933048.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHWN2_003689"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHWN2_003689"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR8933049.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHWN2_003690"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHWN2_003690"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR8933050.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHWN2_003691"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHWN2_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR8933051.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1607 a         1551 c         1608 g         1578 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aagg
//
LOCUS       DAZSNO010000193         6321 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZSNO010000193 DAZSNO010000000
VERSION     DAZSNO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.195"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..378
                     /locus_tag="ACHWN2_003692"
     CDS             <1..378
                     /locus_tag="ACHWN2_003692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933052.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHWN2_003693"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHWN2_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR8933053.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHWN2_003694"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHWN2_003694"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR8933054.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="ACHWN2_003695"
     CDS             complement(1787..2476)
                     /locus_tag="ACHWN2_003695"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR8933055.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHWN2_003696"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHWN2_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR8933056.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHWN2_003697"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHWN2_003697"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR8933057.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHWN2_003698"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHWN2_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR8933058.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1609 a         1709 c         1565 g         1438 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc t
//
LOCUS       DAZSNO010000194         6308 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZSNO010000194 DAZSNO010000000
VERSION     DAZSNO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.196"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..856)
                     /locus_tag="ACHWN2_003699"
     CDS             complement(<1..856)
                     /locus_tag="ACHWN2_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR8933059.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNV"
     gene            964..1242
                     /locus_tag="ACHWN2_003700"
     CDS             964..1242
                     /locus_tag="ACHWN2_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933060.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACHWN2_003701"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="ACHWN2_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR8933061.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACHWN2_003702"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="ACHWN2_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR8933062.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="ACHWN2_003703"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="ACHWN2_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR8933063.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="ACHWN2_003704"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="ACHWN2_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR8933064.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="ACHWN2_003705"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="ACHWN2_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="ACHWN2_003706"
     CDS             complement(4922..5452)
                     /locus_tag="ACHWN2_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR8933065.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="ACHWN2_003707"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="ACHWN2_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR8933066.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1832 a         1318 c         1363 g         1795 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
     6241 tgaggtgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag
     6301 ttcaattc
//
LOCUS       DAZSNO010000195         6088 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZSNO010000195 DAZSNO010000000
VERSION     DAZSNO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.197"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..84
                     /locus_tag="ACHWN2_003708"
     CDS             <1..84
                     /locus_tag="ACHWN2_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933067.1"
                     /translation="YESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACHWN2_003709"
                     /pseudo
     CDS             complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACHWN2_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(1611..2201)
                     /locus_tag="ACHWN2_003710"
     CDS             complement(1611..2201)
                     /locus_tag="ACHWN2_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR8933068.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACHWN2_003711"
     CDS             complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACHWN2_003711"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR8933069.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3179..3715
                     /locus_tag="ACHWN2_003712"
     CDS             3179..3715
                     /locus_tag="ACHWN2_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR8933070.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3756..4616)
                     /locus_tag="ACHWN2_003713"
     CDS             complement(3756..4616)
                     /locus_tag="ACHWN2_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR8933071.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACHWN2_003714"
     CDS             complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACHWN2_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR8933072.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACHWN2_003715"
     CDS             complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACHWN2_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR8933073.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1557 a         1543 c         1509 g         1479 t
ORIGIN      
        1 tatgagtcag tatgcatcgc cgcgatggaa catttaaaaa gtaaagcaac ggcccctata
       61 caaaacggac cgttgccagc ataagaatta acggtgtgcc agttcggcgt cgtcttcgct
      121 atccagaatg gctttatcgg tttgcttcag ccactggctg gtcagcgtgc cagctgtcat
      181 cgagccacta acgtttagcg ccgtacggcc catgtcgata agcggttcaa cggagattaa
      241 cagcgccacc agggttactg gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt
      301 tgcaccacca ccgacaccgg caacgcctgc ggaactaacg gtaacaatac cgaccagcgt
      361 cgcaatccac atcgggtcca gcgggttaat gccaaccgta ggcgcaacca tcaccgcagc
      421 attgccggat acaaaccggc gcagccgttc tgaccaatgg ttgcaccgaa agaggcggcg
      481 aaactggcga tggattcagg aacgcccaga cgacgcgttt gtgcttccac attcagtggg
      541 atagacgcag cactggaacg gctggtaaag gcaaacgtca gcacaggcca taccttacgg
      601 aagtacttca gcggactcac gccattaatg cccagcagaa tgccatgcac agcaaacata
      661 atcagcagac cgaggtagga cgcgacaacg aaacttccca gtttgatgat gtcttgcaag
      721 ttagaacctg caaccacttt ggtcattaga gccagaacgc cgtaaggggt caactgcatg
      781 acaaggcgaa ccagtttcat cacccagctt tgtagggtat cgatagcggt taagacgcgt
      841 tcccctttcg gcgcatcatc cttcagcagt ttcaatgcag ctacgccgag gaatgcggca
      901 aaaattacca cgctgataat tgacgtcgga ttggctccgg taagatcggc aaacgggttt
      961 ttcgggataa aggacaagac cagctgcgga acgctctgat cagagacttt accaacatag
     1021 ttactttcaa tggcgttcag acgtgcagtt tctgcaccac cctgaaccag accttcagcc
     1081 gtcaaaccaa acaggttggt gaccagcaca ccgaccagcg ccgcaatcag cgtggtaaac
     1141 aaaagcgtac cgatggtcag aaaactgatt ttgcctaact gagatgcgtt atgcagacgg
     1201 gcaaccgcgc tcagaataga ggcgaagact aacggcataa cgatcatttg cagcagttga
     1261 acatagccgt taccaacgat gttaaaccac tgtacagaat ctttaagtac ctggctgtca
     1321 gaaccataaa tggtatgcag ggcaaggcca aaaaccacac ccatcaccag acccaccagc
     1381 acttttttcg ccagactcca ctgtttatgg cgggtctgag ccagcgcaaa cagcagtaca
     1441 acgaacacca cgatgttcgc aattaatgga aagttcatcc ccgttctcct gatcttatta
     1501 tttctatcac tgcgtagcag tcatagtgtt ggcgcaagat taacagaact gtgagctggc
     1561 tcttatattc aaatggaatt gcttattcca gaaaggtgat ttttgccttt ttagtattca
     1621 acctgatgct taataagccg atgaaactgg atctgcaagg tattgatcat ttgcgatgac
     1681 caacgaacag cgctgttctc gggtaatgaa tggggcatcc ataccgacca gacaaataat
     1741 gccatgcaga taaaacgttc cagttggttg cccttttggg gaaccaggat aggcaagcgg
     1801 aaacgccagc ggcatggcca gagcagggga acaccggcgg gtgtcagcat atcggcaagt
     1861 atgtggctca aataacccag caccattcct tgtagcgcat cagccggaat gaaccagcct
     1921 tccggaacct taaggtaaaa ggttgccagc agcgcaaata ccgccagcag actgtgggta
     1981 aaaccacgat gcccaaaagc gcgggcgatc ggttttgata tccattttaa tcgctgccca
     2041 agaaacgact ttgggtgatc gatgtccggt aacaaacacg tcaggattgc ggaagggaca
     2101 atatgccacc agtcaccctg tgccagcacg ggcgtcagct cggcattttt ggcaaatacc
     2161 gcacaagcaa tagaaaagag aaggtgacct tccgccgtca tgataaaact cactaaactg
     2221 tcgatacgta cagtatagga tttttataca gtgcacggaa gtggtgacgg tgtaactctt
     2281 tctcttaaat tgacgattaa tccgtcagtt gaatgcaaat tcagataaag aaccaggggc
     2341 agcgtgatca ctgcccctgg tcattaaccg agaaggtctt ttgcggtgag ttctgtcagc
     2401 gatgagagtt tgacgtttgc taatacaaaa cgtggatcat tttgcgcttc tggcgcagga
     2461 acgacgatgg aacgcatgcg ggctgcttta gaagcgatca tgccatttac cgagtcttcc
     2521 agcgccacgc aggtcagtgg gtcaacgccc agttttgctg cgcagtcgag atatacttgc
     2581 ggatgcggct tgctgtaagg cagtttttca gccgaggcga gggcatcgaa actgtcgcgt
     2641 aagtcaaaca tggtcaacac tttttccagc atatgtagtg gtgacgcgga ggccagtccc
     2701 accaataaac cttgttcttt gcataacgca acagcttcgc gcacgcctgg taataatgga
     2761 cgtgtctctt caaccagtga aatggcacgg gcaataaccc gttctactac ttcctgacgg
     2821 cttggcccat tccacggttg ccgggcgtac caaagatcga ccaccatatc gatgcgtaaa
     2881 cctaaggtgt ccggcagctc gttacgacgg gagatatcta cccccaggct tgccatcaca
     2941 tccagttcgg ctcgatccca taaaggttct gagtcgataa gtaatccatc catatcaaaa
     3001 attgcagcaa gaatctgacg cggggttgac attacaacct ctcctttaga gcattatgtg
     3061 taaggtatta agccgtgctt atgattaagc gcctatcata tcgcctttaa agaatgggcg
     3121 aaaacagata tctacaggta aactaagtga aacgacgcgt cttaatcaag gagaactcat
     3181 gacgtatcaa caagctggac gcattgctgt tttgaaaagg attttgggtt gggtgatttt
     3241 tatacctgca ttgatctcta cattgatttc tttgctgaaa tttatgaata cccggcagga
     3301 aaaccaggaa ggcattaatg cggtcatgct cgactttgct catgtcatga tcgatatgat
     3361 gcaggcgaat acgccttttt tgaatctgtt ctggtataac tccccgaccc ctaattttaa
     3421 tggtggtgtg aacgttatgt tctgggtgat tttcatcctg atttttgtcg gactggcgtt
     3481 gcaggattcc ggtgcccgga tgagtcgcca ggcgcgcttc ttgcgggaag gtgttgaaga
     3541 tcaactcatt ctggaaaaag ccaaagggga agaggggcta acgcgtgaac aaattgagtc
     3601 ccgtattgtt gttccgcacc atactatttt cctgcagttt ttctcactat atatcctgcc
     3661 ggttatttgt attgctgcag gttatgtgtt cttttctctg cttgggttta tttaatcaaa
     3721 aggccgcacg gagcggccat tatcctcaac ccatatcatg ctgctaataa tctatccaat
     3781 gactgctgag caataaccaa atgctgaccg ccaaataatc ttgcacgatt taacagcgtg
     3841 tagagttggt aaaccggttg acgttcaagg aaatcggcag gtagcgggga tactgactga
     3901 tagccgtcat agatttgtgg cggctgttca gtatgcagcg gtaacatcgc taggtcgcac
     3961 tctcggtcac cccagtcgca ggcagggtcg aaaatgtacg ggccatccgg acccagcgca
     4021 cagttgccgg accataaatc gccgtgcaac agagaaggct gcggttgatg cgaggccaga
     4081 cgctgctgaa tatgctcgac gatggcgtcg atattgccga aagcgatccc tttctctgct
     4141 gccagttcca actgccagcc aatccgttgt tcagcaaaaa acgttgacca gcgacgttgc
     4201 caggtgttgg gctgtggagt tgtggagagc gagttatcaa aatcgaggcc aaattgtggt
     4261 tggtcactcc attgatgtaa acgcgcaatt tgctgaccaa gaataaatgc gctatgcgca
     4321 tccagcggac gaggtgggag ataatccatc accagaaaac tgtagtcacg gtcagcgcct
     4381 actgcccaaa ccttaggcac ggtgacggtt ttactacgcg acagtaactc cagttggtcg
     4441 gcttcggcgg taaaaccggg aagcagttcc ctttcatcac atttgacgaa aaagtcatgt
     4501 cctgcatagc gcaaatgcca tgcggcatgg acttctccgc caggcagttc attacgcagt
     4561 tcgatttcgc cttcacctaa ctgctcgctc aaaagacgac tgattgcctg ccacatgatg
     4621 tctctcccat gttgtctgcc agatcataag gttagcgcaa gaatgcggtg taaaaacacg
     4681 agctaacgca caccacagac tgattattgc ccaaaaacca tttattttct gttgctattc
     4741 cattcctccc aggtcgtgat ggtgatttca ggatctttgc cggttgcact ttgggtaagc
     4801 cccgaaacca gttctctaat ttcttcttca ctttgggtac tgataagtcc aaaagtgtta
     4861 gtccccagct catggatatt tccctcatca tccgtcatgg tgagcaaaaa accgtcgcga
     4921 gtcaggtaat tattcaactc gttaatttca gtcaatgtat cctcatgcaa catgacggtt
     4981 atgacataat ggacaagatc gccactggcc atagttcacc cctttgttat cacatgactt
     5041 ttgagcatag tcggagaaac gcgttgccga caggttggtg gtgattcccc caatgctggg
     5101 ggaatgtttt tgttagcggg aaaggtaagc gtaaattttt tgcgtatcgt catgggagca
     5161 cagacgtgtt ccctgattga gtgtggctgc actccccgca gctacgccaa aacggaccat
     5221 ctcttcaaga gaggcatttt ctgccagttt cagtgtcatc gcgccgacca tgctgtcacc
     5281 agcgccaacg gtactctggc ttttcaccgg tggtggcacc acctgaatgc agttttcact
     5341 atcgacaccc agcgctcctt gtggaccaag ggaaacgaca acccgtttgg ccttgccgct
     5401 attaacgatt tcctgcgcgg ctttgcggac atcgtctggc tgggtgagtt cgcgattcac
     5461 cagcgcactg agttcttttt ggttaggctt aaccaactcg atgttaccaa tcgccagtgc
     5521 tgcacttaac gcttcgccag aactgtcgat gatgcagcgg atcccttggt tttgcgcagc
     5581 ggaaatcagt tgggttaatt tttccagctt cacacctggc ggcaggcttc cgcttatgac
     5641 caggatggcc ccggattcaa tttccagaac ttgctcttca agctggcgaa actcatcttc
     5701 atttaatgcc gcgcctggca taacaaaacg atactgttca ccgcttgctt ccacatgtac
     5761 gtgtaaattc tgccgggtcc agtctttggc ttctacagta gcgacgggga cattttcatc
     5821 cgccaacagt gaaaccaggt gttcgccggt cgcgccaccc gccgggaaga tcgctgtggc
     5881 actgcctcca agatgggcaa tggcgcgggc gacgttgatg ccgccgcccc cgggttcgaa
     5941 caccggtgcg gtacagcgca gttttccttc gggataaatt tgcggggtaa ttgttgcgct
     6001 atcgagagag ggcgcaagtg tcaacgtata gatacgtacc atcatttcct cctataggct
     6061 gatttcagtc tggcaccgaa tcagcgga
//
LOCUS       DAZSNO010000196         6058 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZSNO010000196 DAZSNO010000000
VERSION     DAZSNO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.198"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..143)
                     /locus_tag="ACHWN2_003716"
     CDS             complement(<1..143)
                     /locus_tag="ACHWN2_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HFR8933074.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="ACHWN2_003717"
     CDS             379..651
                     /locus_tag="ACHWN2_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR8933075.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHWN2_003718"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHWN2_003718"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR8933076.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="ACHWN2_003719"
     CDS             1111..1731
                     /locus_tag="ACHWN2_003719"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR8933077.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="ACHWN2_003720"
                     /pseudo
     CDS             complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="ACHWN2_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3114..3767)
                     /locus_tag="ACHWN2_003721"
     CDS             complement(3114..3767)
                     /locus_tag="ACHWN2_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8933078.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="ACHWN2_003722"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="ACHWN2_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6058)
                     /locus_tag="ACHWN2_003723"
                     /pseudo
     CDS             complement(5211..6058)
                     /locus_tag="ACHWN2_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1621 c         1599 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DAZSNO010000197         6028 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZSNO010000197 DAZSNO010000000
VERSION     DAZSNO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.199"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..302)
                     /gene="yneK"
                     /locus_tag="ACHWN2_003724"
     CDS             complement(<1..302)
                     /gene="yneK"
                     /locus_tag="ACHWN2_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR8933079.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDTLSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKE"
     gene            376..1317
                     /locus_tag="ACHWN2_003725"
                     /pseudo
     CDS             376..1317
                     /locus_tag="ACHWN2_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            1381..2307
                     /gene="glsB"
                     /locus_tag="ACHWN2_003726"
     CDS             1381..2307
                     /gene="glsB"
                     /locus_tag="ACHWN2_003726"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR8933080.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            2307..2651
                     /locus_tag="ACHWN2_003727"
     CDS             2307..2651
                     /locus_tag="ACHWN2_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR8933081.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            2805..4223
                     /locus_tag="ACHWN2_003728"
     CDS             2805..4223
                     /locus_tag="ACHWN2_003728"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR8933082.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEVLKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            4450..5901
                     /gene="uxaB"
                     /locus_tag="ACHWN2_003729"
     CDS             4450..5901
                     /gene="uxaB"
                     /locus_tag="ACHWN2_003729"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR8933083.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1416 a         1365 c         1613 g         1634 t
ORIGIN      
        1 ttttctttgt gtcttccatt cttgacagga taagtgagtt taaactgaac cttttcattg
       61 tatagtgtgt cgttaccccg aaaagaagtc ctctgaatat atgataagtt atcaaggttg
      121 ttaaatatct tttttgtggc tgagttggtg tttaaagacg tgcgccactc ttcatcacat
      181 attatggtac tgagtgtatc aataaaatcc tttaaaactt cttttacttg aggtgcaata
      241 ttacgagcgg gaaaatcatc aggaagagat atgtaattgc agatgcttac tggcgtaacc
      301 atatttgccc catatagtca taagtcatcg gaacatcact atatagggca tttgagctgc
      361 aagaactatc aaaggcaggt gatgcgtggc gctgttccca tcattcttgc aggtaacggc
      421 tacttactta aacatgcgcc gaatgtgatg ggctgtgctc agctcattgc ccaagtgttt
      481 aaagatgcgg gaatcccgca aggcgtatat ggctggctga atgccgacaa cgacggtgtc
      541 agtcagatga ttaaagactc gcgcattgct gctgtcacgg tgaccggaag tgttcgtgct
      601 ggagcggcac tgaaaaaatg cgtactggaa ctgggcggtt cggatccatt tattgtgctt
      661 aacgatgccg atctggaact ggcggttaaa gcggcggtag ccggacgtta tcagaatacc
      721 ggacaggttt gtgcagcggc aaaacgcttt attatcgaag agggaattgc ttcggcattt
      781 accgaacgtt ttgtgtcagc tgcggcagcc ttgaaaatgg gcgatccccg tgatgaagag
      841 aacgctctcg gaccaatggc tcgttttgat ttacgtgatg agctgcatca tcaggtggag
      901 aaaaccctgg cgcagggtgc gcgtttgtta ctgggcgggg aaaagatggc tggagcaggt
      961 aactactatc cgccaacggt tctggcgaat gttaccccag aaatgaccgc gtttcgggaa
     1021 gaaatgtttg gccccgttgc tgcaatcacc attgcgaaag atgcagaaca tgcgctggaa
     1081 ctggctaatg atagtgagtt cggcctttca gcgaccattt ttaccaccga cgaaacacag
     1141 gccagacaga tggcggcacg tctggaatgc ggtggggtgt ttatcaatgg ttattgtgcc
     1201 agcgacgcgc gagtggcctt tggtggcgtg aaaaagagtg gctttggtcg tgagctttcc
     1261 catttcggct tacacgaatt ctgtaatatc cagacagtgt ggaaagaccg gatctgacgc
     1321 gacatattca gctctgatat actcgcaggt cttttcagac ctgcggtcca ggagtagaaa
     1381 gtggcagtcg ccatggataa tgcaatttta gaaaacatct tgcggcaagt gcggccgctc
     1441 attggtcagg gtaaagtcgc ggattatatt ccggcgctgg ctacagtaga cggttcccga
     1501 ttggggattg ctatctgtac cgttgacgga cagctttttc aggccggaga cgcgcaagaa
     1561 cgtttttcca ttcagtctat ttccaaagtg ctgagtctcg ttgtcgccat gcgtcattac
     1621 tccgaagagg aaatctggca acgcgtcggc aaagatccgt ctggatcacc gttcaattct
     1681 ttagtgcaac tggaaatgga acagggtatt ccgcgtaatc cgttcattaa taccggtgcg
     1741 ctggtggtct gcgatatgtt gcaagggcga ctaagcgcac cacggcaacg tatgctggaa
     1801 gtcgtgcgcg gcttaagcgg tgtgtctgat atttcatacg atacggtggt agcgcgttcc
     1861 gaatttgaac attccgcgcg aaatgcggct atcgcctggc tgatgaagtc gtttggcaat
     1921 ttccatcatg acgtgacaac cgttctgcaa aactactttc attactgcgc tctgaaaatg
     1981 agctgtgtag agctggcccg gacgtttgtc tttctggcta atcaggggaa agctattcat
     2041 attgatgaac ctgtggtgac gccaatgcag gcgcggcaaa ttaacgcgct gatggcgacc
     2101 agtggtatgt accagaacgc gggggagttt gcctggcggg tgggactacc ggcgaaatct
     2161 ggcgttggtg gcggtattgt ggcgattgtt ccgcatgaaa tggccatcgc agtctggagt
     2221 ccggaactgg atgatgcagg taactcgctt gcgggtatcg ccgtccttga acaattgacg
     2281 aaacagttag ggcgttcggt ttattaatgc agtctctcga tccactcttc gcgcgtttat
     2341 cccgttcaaa atttcgctct cgctttcgtc tgggcatgaa agagcgtcag tattgcctgg
     2401 agaaaggcgc gccagtcatc gaacaacatg cggcggattt tgtcgctaaa cgtcttgctc
     2461 cggcattacc ggctaatgac ggtaagcaaa cccccatgcg cgggcatccg gtgtttatcg
     2521 ctcagcacgc caccgcgacc tgctgtcgcg gttgtctggc taagtggcat aacattccgc
     2581 agggtgtttc gttaagcgaa gaacaacaac gttatatcgt ggcggtaatc tatcactggc
     2641 tggttgtata gatgaatcag ccatgagaat tgcggaaaac gataaaatag cattcccagg
     2701 gtacctttat ttgccgccgc tgttagcgaa catgctatcc tttatgagaa tttacttatc
     2761 acgttgagat aattgccacc cgcaattgta tggacagata attaatgcat gtacacccca
     2821 tttcaacttt ccgtttgttc caggaaggtc atctgctacg taatagcatc gctatttttg
     2881 cgctaaccac gctgttctat tttattggtg cagagttacg gctggttcac gaactttctc
     2941 ttttctggcc gctgaatggc gtaatggcgg gggtgtttgc ccgctatgtc tggcttaatc
     3001 gactgcatta ctatgcgatc agttatgtgg cgatgctggt ttatgatgcc ataaccaccg
     3061 aatggggact ggtttcactg gttatcaatt tctccaatat gatgtttatt gttaccgtcg
     3121 ccttactggt cgtgcgggat aagcgtctgg ggaaaaataa gtatgagcca gtaagtgctt
     3181 tacggctatt taattactgt ctgattgccg cattattatg cgctattgtc ggggcgattg
     3241 gttcggtcag tattgatagt ctggatttct ggcctttgct tgcggactgg ttcagtgagc
     3301 aattctcaac gggcgtgttg atcgtgcctt gtatgctgac gttggctatt cctggagtgc
     3361 tgccgcgctt taaagcagaa cagatgatgc ccgccatcgc gtttattgtg tcggttattg
     3421 cctcggtagt cattggcgga gcggggagtc tggcgttccc gctgcctgca ttaatctggt
     3481 gtgcagtgcg ctatacgccg caggtaacat gtctgttgac ctttgtcacc ggtgcggtgg
     3541 aaatcgtact ggtggcaaat tcggtgattg atatctcggt cggttcgccg ttctccattc
     3601 cggaaatgtt ctccgcacgt ctcggtattg ccacgatggc gatatgccca attatggttt
     3661 cttttagcgt ggcagcgatc aatttgctaa tgaagcaagt tgcgctgcga gccgactttg
     3721 attttctgac tcaggtttac tcacggtccg gtctttatga ggtgctgaaa agtccatcgc
     3781 tgaaacagac acaacatctg acggtcatgc tgcttgatat cgactatttc aaaagcatta
     3841 acgataacta tggacatgaa tgtggcgata aagtgttaag cgtgtttgcc cggcatattc
     3901 agaagattgt cggtgataag gggctggtgg cgcgcatggg cggcgaagaa tttgctgttg
     3961 cagtgccgtc ggtgaatcct gtcgatggtc tgctaatggc ggaaaaaatc cgtaaaggcg
     4021 ttgaactgca accattcacc tggcaacaaa aaacgcttta tctcacggta agtattggcg
     4081 tcggtagtgg tcgcgcatcg taccgaacgc tgaccgatga ttttaataaa ttgatggtcg
     4141 aagccgatac gtgtctgtat cgctcgaaga aagatgggcg caaccgtacc agcactatgc
     4201 gttacggtga ggaagttgtc tgacatcttt attcattccg cgcgatttga atgcaatgtg
     4261 ttctaccact ttatgatcca ggaaaaggcg tatttcctcg attaaaccat gatccgcgcc
     4321 acacttttct cataaaactg ctcattaatc atcaggagtt gatcaaaaca ggggttcggc
     4381 gtattaaagt ttgctaacct gtcgcaccaa ttcaaacggg ttgtatgact aatcagaagg
     4441 gaacccattg tgaaaacact aaatcgtcgc gattttcccg gtgcacagta tccagaacgt
     4501 atcattcagt ttggtgaagg taacttcctg cgcgcctttg ttgactggca aatcgatctc
     4561 ctgaatgagc acaccgatct gaattctggc gtggtcgttg ttcgtccgat tgaaacttca
     4621 ttcccgccgt cactgagcac gcaggatggt ctgtacacca ccattatccg tggcctgaat
     4681 gagaaggggg aagcggtcag cgacgcgcgt ctgattcgct cggttaatcg tgaaatcagc
     4741 gtctacagtg aatacgatga attcctgaaa ctggcgcaca acccggaaat gcgctttgtt
     4801 ttctctaaca ccaccgaagc gggtatcagc taccatgcgg gcgataaatt cgatgacgcg
     4861 ccagcggtaa gctatccggc aaaactgacc cgtctgctgt tcgaacgctt tagccatttc
     4921 aacggtgcgc tggataaagg ttggatcatc attccgtgtg agttgattga ctacaatggc
     4981 gacgcgttgc gtgaactggt gctgcgctat gcgcaagagt gggcactgcc agaagcattt
     5041 attcaatggc tggatcaggc taacagcttc tgttctacgc tggtggaccg tatcgttacc
     5101 ggttatccgc gcgatgaagt ggctaaactg gaagaagaac tgggttatca cgatggtttc
     5161 ctcgacaccg ctgaacactt ttacctgttt gtgattcagg ggccgaaatc cttagcgact
     5221 gagctgcgcc tggacaaata tccgctcaac gtgctgattg ttgacgatat taaaccgtat
     5281 aaagaacgca aagtggcaat cctcaacggt gcgcacaccg cgctggtgcc ggtggcgttc
     5341 caggcagggc tggataccgt aggtgaagcg atgaacgatg ctgaaatttg cgcattcgtt
     5401 gaaaaagcca tctacgaaga aattattccg gtactggatt tgcctcgtga tgaactggaa
     5461 tctttcgcca gtgcagtcac cgggcgtttc cgtaacccgt acattaagca tcagctgctg
     5521 tctatcgcgc tcaacggtat gaccaagttc cgcacccgca tcctgccgca gctgctggca
     5581 gggcagaagg caaatggcac acttccggcg cgccttactt tcgcattagc ggcattgatt
     5641 gcgttctatc gtggtgagcg taacggagaa acatatccgg tacaggacga tgcacactgg
     5701 ctggaacgtt atcagcaact ctggagccag catcgtgacc gtgttatcgg tacccaggaa
     5761 ctggtagcga ttgtgttggc agaaaaagac cactgggagc aggacctgac gcaagtgcct
     5821 ggtctggtcg agcaggttgc taatgacctg gatgcgattc ttgaaaaagg gatgcgcgaa
     5881 gctgtacgtc cgttgtgcta ataacaccgt tacttctgaa cccggtttcg accgggtttc
     5941 ttatttcact atgttagtta caacatccta tattccttta tcattaacag gtgtcacatg
     6001 cagcgcctgt taatgttgta agcttgac
//
LOCUS       DAZSNO010000198         6022 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZSNO010000198 DAZSNO010000000
VERSION     DAZSNO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.200"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHWN2_003730"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHWN2_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR8933084.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPYNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACHWN2_003731"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACHWN2_003731"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR8933085.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACHWN2_003732"
     CDS             complement(3736..4092)
                     /locus_tag="ACHWN2_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR8933086.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACHWN2_003733"
     CDS             complement(4096..4512)
                     /locus_tag="ACHWN2_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR8933087.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHWN2_003734"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHWN2_003734"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR8933088.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="ACHWN2_003735"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="ACHWN2_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1473 a         1527 c         1532 g         1490 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt atacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt at
//
LOCUS       DAZSNO010000199         5978 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZSNO010000199 DAZSNO010000000
VERSION     DAZSNO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.201"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            100..924
                     /gene="gadX"
                     /locus_tag="ACHWN2_003736"
     CDS             100..924
                     /gene="gadX"
                     /locus_tag="ACHWN2_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR8933089.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1292..2020
                     /gene="gadW"
                     /locus_tag="ACHWN2_003737"
     CDS             1292..2020
                     /gene="gadW"
                     /locus_tag="ACHWN2_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR8933090.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2383..5496)
                     /gene="mdtF"
                     /locus_tag="ACHWN2_003738"
                     /pseudo
     CDS             complement(2383..5496)
                     /gene="mdtF"
                     /locus_tag="ACHWN2_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5521..5778)
                     /locus_tag="ACHWN2_003739"
                     /pseudo
     CDS             complement(5521..5778)
                     /locus_tag="ACHWN2_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1574 a         1407 c         1433 g         1564 t
ORIGIN      
        1 tcccgcttcg tttaaattta tttatcaatc aatttgactt aagagggcgg cgtgctacat
       61 taattaacag taatatgttt atgtaatatt aagtcaacta tgcaatcatt acatgggaat
      121 tgtctaattg cgtacgcaag acataaatat attctcacca tggttaatgg tgaatatcgc
      181 tattttaatg gcggtgacct ggtttttgcg gatgcaagcc aaattcgagt agataagtgt
      241 gttgaaaatt ttgtattagt atcaagggat acgctttcat tatttctgcc gatgctcaag
      301 gaggaggcat taaatcttca tgcacataaa aaaatttctt cattactcgt tcatcactgt
      361 agcagagata ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa gaatcttcgc
      421 tatgcagaaa tgctacgtaa aagagcatta atctttgcgt tgttatctgt ttttcttgag
      481 gatgagcact ttataccgct gcttttgaac gttttgcaac cgaacatgcg aacacgagtt
      541 tgtacggtta tcaataataa tatcgcccat gagtggacac tagcccgaat cgccagcgag
      601 ctgttgatga gtccaagcct gttaaagaaa aaattgcgcg aagaagagac atcatattca
      661 cagttgctta ctgagtgtag aatgcaacgt gctttgcaac ttattgttat acatggcttt
      721 tcaattaagc gagtcgcagt gtcctgtgga tatcacagcg tgtcgtattt catttacgtc
      781 tttcgaaatt attatgggat gacgcccaca gagtatcagg agcgatcggc gcagggattg
      841 ccgaaccgtg actcggcggc aagtattgtt gcgcaaggga atttttacgg cactaaccgt
      901 tctgcggaag gaataagatt atagagtttt actcagacat aaaaaaaacc cggcataggg
      961 gaccgggaag aggatagtct gccgtctcca gactaataaa ccgttataac actccctgtt
     1021 ggcacgggaa actttgtgct ctcagtaagt taaatataac ttttacagga aataagatca
     1081 gccatttttt tataaacata agctatacgc tgtgcgaaaa tatattcgtg ctgcatttac
     1141 ttattatcaa ttaactgtta tgcaaaacta ctttgtggat aaattttggt cctaccaaat
     1201 ctggcagttt ttgcgctaag aaacagtctg tcatcatttc attagtatac tgaaattgaa
     1261 ataatcgcag tatgaaatat aagggataat catgactcat gtctgctcgg tgatcctcat
     1321 tcgtcgttca ttcgatattt atcatgaaca gcaaaaaata tcgctgcata acgagagtat
     1381 cctgctgctg gagaaaaatt tggcagacga ttttgcgttt tgttcaccgg atacgcgacg
     1441 actggatatc gatgagctga cagtttgcca ttacttacaa aatattcgtc agctaccacg
     1501 caatttaggg ttacatagca aagaccgtct gttaattaac cagtcacccc ccatgccgct
     1561 ggtgacggcg atttttgata gcttcaatga atccggggta aattcaccga tactgagcaa
     1621 tatgctctac ctttcctgtt tatcgatgtt ttctcataag aaagaactga tccccttact
     1681 tttcaatagc atcagcactg tttcaggaaa agttgaacgc cttattagct ttgatatcgc
     1741 caaacgttgg tatctgcgcg atatcgcgga aagaatgtat accagcgaga gtctcatcaa
     1801 aaaaaagttg caggatgaaa atacctgttt cagtaaaata ttactcgctt ccaggatgtc
     1861 gatggccaga cgattactcg agttacgtca aattcctctg catactattg cggaaaaatg
     1921 tggctatagc agtacgtcgt actttataaa cacatttcga caatattatg gtgtaacgcc
     1981 acatcagttt gcgcaacatt cgccaggtac cttttcctga catattttgc atttgaatat
     2041 tggtcaggat ctcacacctg cttcatgtga aactcttccc tgatgatttc tgccgggcta
     2101 ccggctagtt ctctttcgca gtttgccagg cggtataggt atttttagga ggaaaagact
     2161 cggcatgttt gggattatta agctgacaat tcatacgatt acgggaatgt gggtcagtat
     2221 tgtgttgttc aaattgatga cgaatggctg gagtggtttc tactttcagt gctgtgtact
     2281 aagcctggta ttcctgacgg ttagctggtt gttgagtggt gaatggttag caggaaagag
     2341 taaggctgaa ccttcacgtt caaccttact ctcatttaca cgttacgctt ttttaaagcg
     2401 ggcaaagaga tgttccacta caacgaaaaa gaccggaacg aagtaaattg ccagcactgt
     2461 tgcggcaaac atcccgccca tcacgccggt acctaccgcg ttttgcgcgc cagaaccggc
     2521 accatgactg ataaccagcg gcagcacgcc gagaataaag gccagagagg tcatcaggat
     2581 tgggcgtaaa cgcatccgcg ccgcttcgat gattgcctct atcggcgttt tcccttcttt
     2641 ctgcatcatc tcaacggcaa attcgacaat caggatggcg ttcttggcgg aaagcccgat
     2701 ggtggtcagc aaaccaacct ggaagtagac gtcattactt aagccgcgca gatcggtggc
     2761 cagtaatgcg ccaacgacgc ctaacggaac aaccaacatc accgagaacg gaattgacca
     2821 gctctcatag agtgcggcga gggcgaggaa caccacgacc agtgaaatgg catacagtgc
     2881 aggagcctgg tttgaggata acgcttcctg atacgacagt ccggtccatg agtagccgac
     2941 gcctgccgga agtttagcga ccaggtctgc cataaatttc atagcgtcac cggtactttt
     3001 cccggccgcc gcttcaccta aaatctccat tgacgggatg ccgttgtagc gttccaatcg
     3061 cggtgaacca taggtccatt cggtagacga gtaggcagaa agcggtgcca tcgtgccaga
     3121 ggcgttgcgt acataccatt ggttgatgtt atccggcaac atacggaacg gcgtgcctgc
     3181 ctggacatac acttttttca cccgcccctg gttgaggaag tcgttcacgt agctgctgcc
     3241 gaaggcggtg gaaattgtct ggttgatatc agacagcgcc acacccattg cctcagcttt
     3301 cgcggcgttg acgttcactt tgaacatcgg cgtatcttcc aggccgttcg ggcgtacccc
     3361 ggtgacctgg tcaggcgatt gcgctgccag tgataacagc tcgtttcgtg cctaggttag
     3421 tttttcgtgc cccaggttac cgttgtccag cagttccata tcaaaacctg acgcggtacc
     3481 cagttcagcc accgcgggta agttgaacgg gaagacgacg gctttattga tactgcttaa
     3541 cgcaatcatt gcccgctgaa tgatcgcggt aaccgagttt tcctcaccga cacgttcaga
     3601 ccacggcttg agactgataa acgccaggcc gttgttttgc ccctggccgc tgaagccaaa
     3661 gccgccaacg gtaaacaccg actgaacatt atctttctct tttgtaagat aataatccgt
     3721 cacctgttgc agtactttcg tggtgttaac catggtggca ccggaaggta actgcgcggt
     3781 ggtcataaat accccctgat cctcttctgg taagaaagag gtcggtgtac gcaggaacag
     3841 caccgccatc ccggcgcaaa tcagcaggta gaccaccatg tagcgaccgg tacaacgcaa
     3901 cagcgagcgg gtgctatcgg tatagtgctg agttgatttt tcaaacagcg tgttgaagcg
     3961 tgcgaacagg gcgttaggtt tgtgaccgcc ttccggcgtg gctttcagaa tggtggcgca
     4021 cagggcaggg gtcaggctca ttgccacaaa tactgaaagc cgcatggagg agatcagcgt
     4081 gatggagaac tggcggtaga tctccccggt tgcaccgctc ataaaggcca tcggcataaa
     4141 cactgcagaa agaacaacgg caatcccgac cagcgcacgt tggatctgcc ccatcgattt
     4201 atgcgtcgct tccttcggcg gtagcttatc ttccgcaatg acacgctcga cgttctccac
     4261 caccacgatg gcgtcatcca ccagtaaccc tatcgccagc accatcccga acatagtcaa
     4321 cgtgttgatg gtgaaaccga ccgccgacaa gatcgcaaac gtcccgagaa taaccaccgg
     4381 tacggcaatt gtcgggatga tggtggcacg gaaattctgc aaaaacagat aaatgaccag
     4441 gaagacgagg atgatagcct caaccagtgt tttgaaaact tcctgaatag aaatttcgat
     4501 aaacggcgtg gtgtcgtaag gataaaccgt cttcagactt gcggggaaat aggctgataa
     4561 gcggttcagc tcctctttga ctgcccgcga ggtatccagg gcgtttgctc cggcagccag
     4621 tttgatggcg atcccggcaa caggtttgcc gttatagcgc gccacggtgg aataatcttc
     4681 cgccccaagt tcgacgcggg cgacatcacg cagcagcact tgcgaaccat cttgctgaac
     4741 tttcaacagg attttgccaa attcttccgg cgtttgcaga cgcgtctgca caatgatcga
     4801 ggcgtttagt tgctggtctg ccgcctgtgg catgccaccc agttgaccac cagaaatctg
     4861 gttgttttgc accttaatct gggaaataac atcggaaggt accaggttat atttattgag
     4921 tttttgcggg tccagccaga tacgcatggc gtactcggaa ccaaagagct gtacgctacc
     4981 aacgcccgcg gtacggctta gcgggtcttt gatattagac gctacatagt ccgcgatatc
     5041 gtactggttg aggctgccgt tatcagaaat aaacgccgct accatcagga tattactgct
     5101 cgacttatcg acgctaatcc cctgttgctg caccgcttca ggtaatgaag gcatagcgag
     5161 ttgcagttta ttttgcacct gaacctgtgc gatatcagga gatgccccag tctcgaaggt
     5221 cagagtgata gaggcattac ccgccgcatc actggttgaa gacatgtaca tcaggccatc
     5281 aagcccattc atattttgct caatcacctg agtgaccgag tcttctaccg tttgcgcatc
     5341 ggcacccgga taggtagcgc tgacggtaat ggtcggtggc gcaatctgcg gatactgcgc
     5401 aaccggtaag ttcatgatcg ccagaccacc tgcaagcatc ataataatgg caagtaccca
     5461 ggcaaaaacc gggcgatcaa taaaatagtt agccatgaaa gtccccttaa gcctgcaacg
     5521 ttattgtttc gattcggtgc tggcgttttc ctggctggag gaaattgctc gtgctttgat
     5581 acccggacga atgcgttgca aaccggaaac gatcacccga tcgccagcct gcaagccaga
     5641 ggtgacaacc cactggtcgc cgatggcttt gctggcttca atttcgcgta gctgcacgac
     5701 atcgtcttta tccagaatga gcgccgttgc tttaccctgg gcgttgtggg tgacgccttc
     5761 ctgcggcacc agtaatataa agcgcgcaaa attattcaag atatttttga aaatcattca
     5821 agataggcta tcgcgtgtga tagcaaaaac attcaagata aaaatgcttg attattaagc
     5881 aatcaggaat gggagtatta agtttcgtgt ttccagtaaa accagagagt attgaaatag
     5941 ctatcaccta actttatcag gcgatagctg gtagatga
//
LOCUS       DAZSNO010000200         5799 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZSNO010000200 DAZSNO010000000
VERSION     DAZSNO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.202"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..948
                     /gene="lpfD-O113"
                     /locus_tag="ACHWN2_003740"
     CDS             <1..948
                     /gene="lpfD-O113"
                     /locus_tag="ACHWN2_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR8933091.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
     gene            1196..2236
                     /gene="pstS"
                     /locus_tag="ACHWN2_003741"
     CDS             1196..2236
                     /gene="pstS"
                     /locus_tag="ACHWN2_003741"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR8933092.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2323..3282
                     /gene="pstC"
                     /locus_tag="ACHWN2_003742"
     CDS             2323..3282
                     /gene="pstC"
                     /locus_tag="ACHWN2_003742"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR8933093.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3282..4172
                     /gene="pstA"
                     /locus_tag="ACHWN2_003743"
     CDS             3282..4172
                     /gene="pstA"
                     /locus_tag="ACHWN2_003743"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR8933094.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4264..5037
                     /gene="pstB"
                     /locus_tag="ACHWN2_003744"
     CDS             4264..5037
                     /gene="pstB"
                     /locus_tag="ACHWN2_003744"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR8933095.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRVEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5052..5777
                     /gene="phoU"
                     /locus_tag="ACHWN2_003745"
     CDS             5052..5777
                     /gene="phoU"
                     /locus_tag="ACHWN2_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR8933096.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1437 a         1396 c         1516 g         1450 t
ORIGIN      
        1 tcgggatatc ttattaccgc gaacgaaaat aaggttggaa cgacatttaa caccactgta
       61 actaatggat cctcatatct tggacgctgt cattgtgata ctggtaacgt aggagaattc
      121 ccctatatct attatacgtc aaaaataaac caggcattaa cttatgctgg ggttcactct
      181 aatattaatt attatgatct taatccgaac cttgatgttg ggattgctat agatattctt
      241 ggggttgggt atgtgaatgc accttttgaa tatcatgcta acaacccgtc tggtaataca
      301 aagtacaatt gcaaccgcat tgaaccttta agtatatcca gtggggctaa agcgatagta
      361 tatttttata tcaagaagac atttgcagga aaattgatta ttcctgaaac aaagatagtg
      421 acattgtatg gaacaattag ccgtgacacc ccggtggatt actcacagcc gatggctgat
      481 gtttatattc ggggcgatat tacagctccg caaagttgcg aaataaataa tttaaagcca
      541 gtttgttttg attttaaaga aataccagct gcagattttt catctgttgt tggaagtgcg
      601 gtaacaacac ataaaattac taaaacagtc actattgagt gtgaaaacct ggggatactg
      661 aataccgatg atatcagtac gtctttttat gctaccgaac ccaatacgga caattcaatg
      721 gtcgtaactt caaactctaa cgtggggata aaaatttacg acaaaaataa taaggaaatc
      781 aaagtgaacg gtggtgagtt gccaacagac atggataaat caacagtgta tggtgaaaaa
      841 tcaggtagtg taactttttc agctgctcct gcgagcctta ctggagctcg cccagccccg
      901 gggcaattta ctgcaacagc gacgataact gttgagattg tgcgctaata ggtctgacaa
      961 cgattgttca tcacatcgtt gtcatctcac ccatttcttt atcactttta aatgcatcat
     1021 gacaaaaaga tggcatgtgg aattttcatc ttcacattta aaatcagtaa gttatttggt
     1081 aattcctttc tgtcataaaa atgtcatatt ccttatatat aactgtcacc tgtttgtcct
     1141 attttgcttc tcgtagccaa caaacaatgc tttatgaatc ctcccaggag acattatgaa
     1201 agttatgcgt accaccgtcg caactgttgt cgccgcgacc ttatcgatga gtgctttctc
     1261 tgtgtttgca gaagcaagcc tgacaggtgc aggtgcaacc ttccctgcgc cggtgtatgc
     1321 caaatgggct gacacttacc agaaagaaac cggtaataaa gttaactacc agggtatcgg
     1381 ttcttccggt ggcgtaaaac agattatcgc taataccgtt gattttggtg cctctgacgc
     1441 gccgctgtct gacgaaaaac tggctcagga aggtctgttc cagttcccga ccgtgattgg
     1501 cggcgtggtg ctggcggtta acattccagg gctgaagtct ggcgaactgg tgctggatgg
     1561 taaaaccctc ggcgacatct acctgggcaa aatcaagaag tgggatgatg aagccatcgc
     1621 caaactgaat ccgggtctga aactgccttc acaaaacatt gctgtagtac gccgcgcaga
     1681 tggctccggg acttccttcg tcttcaccag ctacctggcg aaagtgaacg aagagtggaa
     1741 aaacaacgtt ggtactggct ctaccgtaaa atggccgatc ggtctgggcg gtaaaggtaa
     1801 cgacggtatc gccgcgttcg ttcagcgtct gccgggtgca attggttatg ttgaatatgc
     1861 ttacgcgaag cagaacaacc tggcgtacac caaactgatc tccgctgatg gtaaaccggt
     1921 tagtccgacc gaagaaaact tcgctaatgc agcaaaaggt gcagactgga gcaaaacctt
     1981 cgctcaggat ctgaccaacc agaaaggcga agatgcatgg cctattacct ctaccacgtt
     2041 cattctgatc cacaaagatc agaagaaacc agaacaaggc acagaggtgc tgaaattctt
     2101 cgactgggcg tacaaaaccg gggctaaaca ggcgaacgac ctggattacg ccagcctgcc
     2161 ggatagtgta gttgaacagg ttcgcgctgc gtggaagacc aatattaaag acagtagcgg
     2221 taagccgctg tactaataaa actccaggcc gggtacggtg ttttacgccg catccggcat
     2281 tacaaaatga ctttgtaaac gcgtttaact gaagagtaac ttatggctgc aaccaagccc
     2341 gcttttaacc caccgggtaa aaagggcgac ataattttca gcgtgctggt aaaactggcg
     2401 gcgctgattg tgctattgat gttgggtggc attattgtct ctctgatcat ctcctcctgg
     2461 ccgagcattc agaaatttgg tctggctttc ctatggacca aagagtggga tgcaccgaac
     2521 gatatctacg gggcgctggt gccgatctac ggtacgttgg tgacttcgtt tatcgcgctg
     2581 ctgatcgccg tcccggtgag tttcggtatc gccctgttcc tgactgagct tgcgcctggc
     2641 tggctgaaac gcccgctggg tatcgccatt gagctgctgg cagccattcc aagtatcgtt
     2701 tacggcatgt ggggcctgtt tatctttgcg ccgctgttcg ccgtttactt tcaggagccg
     2761 gtcggcaata tcatgtcgaa tatcccgatt gttggcgcgc tgttctctgg ccccgcattt
     2821 ggtatcggta tcctcgcggc aggcgtgatc ctcgccatca tgattattcc gtacattgcg
     2881 gcggtaatgc gtgatgtgtt cgaacaaacc ccggtgatga tgaaagagtc ggcctacggt
     2941 attggctgca ccacctggga agttatctgg cgtatcgttc ttccgttcac caaaaatggt
     3001 gttatcggcg gcatcatgct ggggctgggc cgcgcgctcg gtgaaaccat ggcggtgacc
     3061 tttatcatcg gtaacaccta ccagctcgac agcgcctcgc tgtacatgcc gggcaatagt
     3121 atcacctctg cgctggcgaa cgaatttgcg gaagcggaat ccggtctgca cgttgccgca
     3181 ctgatggaac tgggcctgat cctgtttgtg attaccttca tcgtcctcgc cgcatcgaag
     3241 tttatgatta tgcgcctggc taagaatgag ggggcacgct aatggctatg gttgaaatgc
     3301 aaaccactgc ggcgctggct gaatctcgcc gcaaaatgca ggcgcgtcgc cgcctcaaaa
     3361 accgtattgc gctgacgctc tcgatggcga cgatggcctt cggcctgttc tggctgatct
     3421 ggattttaat gtccaccatc actcgcggta tcgacggtat gtcgctggcg ctgttcactg
     3481 aaatgacgcc gccgcccaat acggaaggtg gtggtctggc gaacgccctg gcgggtagcg
     3541 ggctgttaat tttgtgggcc acggtattcg gtacgccgct gggcattatg gcggggattt
     3601 atctggcgga atatggtcgt aaatcctggc tggcagaagt gattcgcttc attaacgaca
     3661 ttctgctctc cgcgccgtcg attgtggttg gtctgtttgt ctacaccatc gtggtggcac
     3721 agatggagca cttctccggc tgggcgggcg taattgcgct ggcgctgttg caggtgccga
     3781 tcgttatccg caccactgag aacatgctga aactggtgcc ggacagcctg cgtgaagcgg
     3841 cttatgcgct gggtacaccg aagtggaaga tgatctccgc gattacgctg aaagcgtcgg
     3901 tgtccgggat tatgaccggt atcctgctgg cgattgcccg tattgccggt gaaaccgcgc
     3961 cgctgctgtt taccgcgctc tccaaccagt tctggagcac ggacatgatg cagccgatcg
     4021 ccaacctgcc ggtgacgatc tttaagtttg cgatgagccc gtttgcggaa tggcaacaac
     4081 tggcctgggc cggggtattg atcattaccc tgtgcgtcct gctgctgaac attctggcgc
     4141 gcgttgtttt tgcgaagaat aaacacggtt aacacttttc cggatgcggc gtgaacgcct
     4201 tacccggccc tacggtaagc ctgattagcg aaaagcatca ggtacgatga ggaaaagatt
     4261 gcaatgagta tggttgaaac tgccccgagt aaaattcagg ttcgtaattt gaacttctac
     4321 tacggcaaat tccatgccct gaaaaacatc aacctggata tcgccaaaaa ccaggttacg
     4381 gcgtttatcg ggccgtccgg ctgcggtaaa tcgacgctgc tgcgtacctt caacaaaatg
     4441 tttgaactgt acccggagca gcgtgtggaa ggtgaaattc tgcttgatgg cgacaacatc
     4501 ctgaccaact ctcaggatat cgccctgctg cgtgcgaaag tgggcatggt gttccagaaa
     4561 ccgacgccgt tcccgatgtc catctacgac aacatcgcct ttggcgttcg tctgtttgag
     4621 aagctctccc gtgccgacat ggacgagcgc gtgcagtggg cattgaccaa agccgcattg
     4681 tggaacgaaa ccaaagataa attgcaccag agcggttact ctctctctgg tggtcagcaa
     4741 cagcgtctgt gtattgcgcg tggtatcgcc attcgcccgg aagtgctgct gctcgacgaa
     4801 ccgtgttcgg cgctcgaccc catctctacc gggcgtattg aagagctgat caccgaactg
     4861 aagcaggatt acaccgtggt gatcgtcacc cacaacatgc agcaggctgc gcgttgttcc
     4921 gaccacacgg catttatgta cctgggcgaa ttgattgagt tcagcaacac ggacgatctg
     4981 ttcaccaagc cagcgaagaa acaaacagaa gactacatca ccggtcgtta cggttgattc
     5041 aggagtgcgt tatggacagt ctcaatctta ataaacatat ttccggccag ttcaacgccg
     5101 aactggaaag tatccgcacg caggtgatga ccatgggcgg catggtggag cagcagcttt
     5161 ctgatgcaat caccgcgatg cataaccagg acagcgatct ggcgaagcgc gtgatcgaag
     5221 gcgacaagaa cgtcaacatg atggaagtgg cgatcgatga agcctgcgtg cgcattatcg
     5281 ccaaacgtca gccgacggcg agcgacctgc gactggttat ggtgatcagt aaaaccattg
     5341 ccgagctgga gcgtattggc gacgtggcgg acaaaatctg ccgtactgcg ctggagaaat
     5401 tctcccagca gcatcagccg ttgctggtaa gtctggagtc gctgggccgt cataccatcc
     5461 agatgctgca cgacgtgctg gacgcgttcg cgcggatgga cattgacgaa gcggtacgta
     5521 tttatcgtga agataaaaaa gtcgatcagg aatacgaagg tattgttcgt caactgatga
     5581 cctacatgat ggaagattcg cgcactattc cgagcgtact taccgcgctg ttctgtgcgc
     5641 gttcaatcga acgtatcggc gaccgctgcc agaatatttg tgagtttatc ttctactacg
     5701 tgaagggtca ggatttccgt catgtaggcg gtgatgagct ggataaactg ctggcgggaa
     5761 aagatagcga caaataattc accagacaaa tcccaataa
//
LOCUS       DAZSNO010000201         5738 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZSNO010000201 DAZSNO010000000
VERSION     DAZSNO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.203"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..241
                     /locus_tag="ACHWN2_003746"
     CDS             <1..241
                     /locus_tag="ACHWN2_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933097.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="ACHWN2_003747"
     CDS             complement(238..1179)
                     /locus_tag="ACHWN2_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFR8933098.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="ACHWN2_003748"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="ACHWN2_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="ACHWN2_003749"
     CDS             complement(1684..2904)
                     /locus_tag="ACHWN2_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR8933099.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="ACHWN2_003750"
     CDS             complement(2908..3651)
                     /locus_tag="ACHWN2_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR8933100.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="ACHWN2_003751"
     CDS             complement(3542..5008)
                     /locus_tag="ACHWN2_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFR8933101.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5738)
                     /locus_tag="ACHWN2_003752"
                     /pseudo
     CDS             complement(5008..5738)
                     /locus_tag="ACHWN2_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1225 a         1618 c         1436 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccgggacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAZSNO010000202         5735 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZSNO010000202 DAZSNO010000000
VERSION     DAZSNO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.204"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            113..1021
                     /locus_tag="ACHWN2_003753"
     CDS             113..1021
                     /locus_tag="ACHWN2_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFR8933102.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMLPGSTVPFISTK"
     gene            complement(1067..3058)
                     /gene="tkt"
                     /locus_tag="ACHWN2_003754"
     CDS             complement(1067..3058)
                     /gene="tkt"
                     /locus_tag="ACHWN2_003754"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR8933103.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3336..4094
                     /gene="loiP"
                     /locus_tag="ACHWN2_003755"
                     /gene_synonym="yggG"
     CDS             3336..4094
                     /gene="loiP"
                     /locus_tag="ACHWN2_003755"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR8933104.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4300..5220)
                     /gene="speB"
                     /locus_tag="ACHWN2_003756"
     CDS             complement(4300..5220)
                     /gene="speB"
                     /locus_tag="ACHWN2_003756"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR8933105.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5356..>5735)
                     /locus_tag="ACHWN2_003757"
     CDS             complement(5356..>5735)
                     /locus_tag="ACHWN2_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933106.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1376 a         1531 c         1515 g         1313 t
ORIGIN      
        1 aatgcaatgt tttttcgcta taccccttta tagattctga attctcacct gtcggctgtt
       61 ttctcctcat ctcatgtgac atattcccat actttactca aggaagattg tcatgaaagc
      121 acctgtttca ttaacctcat ttcgcccaca aaaatctctt gctatcgcca ttggcgtgct
      181 tgccgtggtt gtgctgccat ttctctcgta ttacaccgtt aatgaaggtg aacgcggcat
      241 tttgctgcgc tacggaaaaa tcgtaaaagt tgccgaaccg gggctgggtt ttaagatccc
      301 ttttatggaa agcgtggaga aaatttccac gcgtaatcag gctgtggtct atcagggatt
      361 acaggcctac agccgcgacc agcaaccggc acaaatgaca gtatcagtca gcttccacat
      421 taaaccttca gaagcaggcg cggtttatac cacctacaac actattgaag cattaaaaga
      481 tcgtctaatt gtccgtcagt tgccaactca gctggagaac attttcggcc aatataccgc
      541 catcagcgcc gtacaggatc gtacaaaatt ggttcaggat ctgcaaaatg ccatgcgcaa
      601 agccgttgtt gggccagtgg taattgacgg ggtccagatt gaaaatattg atttttctga
      661 cgcttatgaa aagtcgattg aaaaccgaat gaaagcagaa gtcgccattg ccacgcgtaa
      721 gcaaaatctg gaaacagaga aaatccaggc gcaaatagcg gtaactcagg cccaggcaga
      781 agcagacagt aaactggcgg cagccaaagc agaagcggaa actattcgcg taagaggcgc
      841 ggcagaggct gaaactattc gtttaaaaag tgcggctgaa gcagaagcaa tccgcctgcg
      901 cggtgaagct ctgcgggata atcccggact tgtagccctc accactgccg aacgttggga
      961 cggtaaactg cccgatacca tgctccccgg cagcacagtt ccgtttatct ctactaagta
     1021 aaaaaagggc cgctttcgcg gtccttctca caaaacgtct tactgattac agcagcgctt
     1081 ttgctttcgc aacaacgtta tcaacagtga agccgaactc ttcaaacagc agctctgccg
     1141 gagcagattc accgaaggtg gtcatgccga cgatagcgcc gttcaggcca acatacttgt
     1201 accagtagtc agcaataccc gcttctaccg caacgcgtgc agtaaccgct ttcggcagta
     1261 cggattcacg gtaagccgca tcctgcttgt caaatgcgtc ggtagacggc atggacacca
     1321 cgcgcgcttt cacgccttcg gcagtcagtt tttcgtaggc agcaaccgcc agttcaactt
     1381 cagaaccggt ggcgatgaag atcagttccg gctgaccagc acagtctttc agcacataac
     1441 caccgcgcgc gatgtttgcc agctgctctt cagtacgttc ctgctgcgcc aggttctgac
     1501 gggagaggat aagcgcagtc gggccgtcct gacgctcaac gccgtatttc cacgcgaccg
     1561 cggattcaac ctggtcacac ggacgccatg tagacatgtt cggggtcacg cgcagagaag
     1621 cgacctgctc taccggctgg tgagtcgggc catcttcgcc cagaccgatg gagtcgtggg
     1681 tgtaaaccat cacctggcgc tgtttcatca gcgcagccat acgtacggcg ttacgtgcgt
     1741 attccacgaa catcaggaag gtggaggtgt acggcaggaa gccaccgtgc agggagatac
     1801 cgttagcaat cgcggtcata ccgaactcgc gaacaccgta gtggatgtag ttacctgcag
     1861 catcttcgtt gattgcttta gaaccagacc acagggtcag gttagacggt gccaggtcag
     1921 cagagccgcc gaggaattca ggcaacagcg ggccgaacgc ttcgatagca ttctgcgacg
     1981 ctttacggct ggcgattttc gccggattag cctgcagttt agcgataaac tctttcgctt
     2041 tggcgtcgaa gtcagacggc atttcgcctt tcatacggcg ggtaaattca gccgcttcct
     2101 gcggataagc tttcgcgtaa gccgcaaact tctcattcca tgcagattct ttcgcctggc
     2161 ctgcttcttt cgcatcccac tgagcataga tttcagacgg gatttcgaac ggtgcatatt
     2221 tccagcccag ctgttcgcgg gtcagggcaa tttcagcgtc gcccagcggc gcaccgtggg
     2281 agtcgtgagt accggctttg ttcggggaac cgaaaccgat gatggttttg cacatcagca
     2341 gggacggttt gtcagtcact gcgcgcgctt cttcaactgc gcgtttgatg gatgccgcgt
     2401 catgaccgtc gatgtcgcga ataacgtgcc agccgtaagc ttcgaaacgc attgctgtgt
     2461 cgtcggtgaa ccagccttca acgtgaccat cgatagaaat accgttgtca tcgtagaacg
     2521 caatcagttt acccagcttc agcgtacccg ccagagagca aacttcgtgg gagatgcctt
     2581 ccatcatgca gccgtcgccc atgaaggcgt aggtgtagtg gtcgacaatg tcgtggcccg
     2641 gacggttaaa ctgcgccgcc agcgtttttt ctgcaatcgc cataccgact gcgttggcaa
     2701 taccctgacc cagcggaccg gtggtggttt ccacaccagc ggtataacct acttccggat
     2761 ggcccggagt tttagagtgc agctgacgga agtttttcag ttcttccatc ggcagatcgt
     2821 aaccggtgag gtgcagcagg ctgtagatca gcatggagcc gtggccgttg gacagcacga
     2881 agcggtcacg gtcagcccag gacggattct gcgggttgtg tttcaggaaa tcacgccaca
     2941 ggacttcggc aatgtcagcc atacccatag gggcacccgg gtgaccggat ttggctttct
     3001 gtactgcgtc catgctcagc gcacgaatag cattggcaag ctctttacgt gaggacattt
     3061 tgactccaga tcggatgatg aagggcacgc ccttaacgac ttgacgacag cgcgttttgg
     3121 gctacgccgg aaaatttgcc aacaatttac cgcaagccgc gcgtcatgta catggaacat
     3181 ccttttgccg cttcagaaat ctctggatca tgctcgcatg ttgcgcaatc tgctcgcccg
     3241 tccgctgcgc ttttccttat actgagactg agcgtcgatt cacctgcaaa cggcgcattt
     3301 ttagaataat cctgaccttg tgcggaagag aaaacatgaa aattcgcgcc ttattggtag
     3361 caatgagcgt ggcaacggta ctgaccggtt gccagaatat ggactccaac ggactgctct
     3421 catcaggagc ggaagctttt caggcttaca gtttgagtga tgcgcaggtg aaaaccctga
     3481 gcgatcaggc atgtcaggag atggacagca aggcgacgat tgcgccagcc aatagcgaat
     3541 acgctaaacg tctgacaact attgccaatg cgctaggcaa caatatcaac ggtcagccgg
     3601 tgaattacaa agtgtatatg gcgaaggatg tgaacgcctt tgcaatggct aacggctgta
     3661 tccgcgtcta tagcgggctg atggatatga tgacagataa cgaagtcgaa gcggtgatcg
     3721 gtcacgagat ggggcacgtg gcgttaggcc atgtgaagaa agggatgcag gtggcgcttg
     3781 gtacaaatgc cgtgcgagta gctgcggcct ctgcgggcgg gattgtcggc agtttatctc
     3841 aatcacaact tggtgatctg ggcgagaaat tagtcaattc gcaattctcc cagcgccagg
     3901 aagcagaagc cgatgattat tcttacgatc ttctgcacca acgcggcatc agcccggcag
     3961 gtcttgccac cagctttgaa aaactggcga agctggaaga aggtcgtcaa agctcaatgt
     4021 ttgacgacca tcctgcatcc gccgaacgtg cccagcatat tcgcgatcgc atcagcgcgg
     4081 atgggattaa gtaaagccta gtggtgtcga acgtaggtca gataaggcgt tcacgccgca
     4141 tctgacatta atggcacatt tgttaccttg tgcgcattgc cggatgcgat gctggcgcat
     4201 cttatccggc ctacgggtgc cgaacgtagg tcggataagg cgttcacgcc gcatccgaca
     4261 ttaatggcac gttttacccg tgcgcatcgc atctggtgct tactcgccct ttttcgccgc
     4321 ctgaatatac agcatctcca gcgccagcgt cgccgcagcc agagcggtga tttccgactg
     4381 atcgtatgcc ggagccactt ccactacgtc catcccaacg atgttgagat ctttcaggcc
     4441 gcgtaccagt ttaatggcgc gatcggaggt caggccgcca atcactggcg taccggttcc
     4501 cggcgcgaaa gcaggatcca ggcagtcgat atcaaaagtc aggtaaaccg gcatatcacc
     4561 cacaatctgt ttcacctggg cgataacgtc atccacgctg cgatcgttca cctggcaggc
     4621 gtccagcacg gtaaagccgt tgtctttatc aaactcggta cgaataccaa tctgcacgga
     4681 atgattcggg tcgatcagac cttctttcgg cgcggtatag aacatagtgc cgtggtcaaa
     4741 ttcacaaccg ttcgcatagg tatcggtgtg ggcgtcaaag tgtaccagcg ccattttgcc
     4801 gaaatgcttc gcatgagcac gcagcagcgg cagcgtaaca aagtggtcac caccgaagga
     4861 gagcatacgc ttaccggcag ccagcagctt ctcggcgtgc gcctgcagct tttcgctcat
     4921 ctcacgggca tcgccaaagg catataccag atcgccgcag tccacgacgt tcagacgctc
     4981 acgcatgtcg aaattccacg ggaagcggtt gtgttcccag gccagattcg tcgaaacctg
     5041 acggatcgct gccggaccgt ggcgaccacc cgcacgacca gaagtggcca tatcgaacgg
     5101 cacgccagta atcacccagt ctgcatcgct gtcatacggc tggaagttca tcggcaggcg
     5161 taaaaaacca aaggcattgg aaaccagtga gttatcgtat tgatgaccta aggtgctcat
     5221 ggatggactc ctattacaaa gtcgatataa aaaaaccctt ccgcgtcgtt aggcccgacg
     5281 aggaagggtt ggatttgcga aacacaagtt tgtgctaatt atcgccttaa atgggcgagg
     5341 catcaagcga ggttgctatt tccgacacgg tggcggcgca tagccattgt tatctgccgg
     5401 aaaggcttgt ccagagcacg gcggcagcgc gttttgcgcc gggccttgta attcaaaggc
     5461 ggcaaagccg attaccccgg tattacgcac atcgccttgc gccgaattgg ctgcgtagga
     5521 atcagcaacg ttggcgaact gaaaagccgc ttcagtatgt ttatctttgc ggaatccttt
     5581 aatcactaac gaatctccgg cattcacaat atagccgttg ttattcagcg aaccttgctt
     5641 gccgttcagc acatccaggc catcagccgt agcaacgatc tcgtagttgg tgttacgact
     5701 gtaattacgg acatataatt gataattcat tccga
//
LOCUS       DAZSNO010000203         5702 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZSNO010000203 DAZSNO010000000
VERSION     DAZSNO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.205"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..170)
                     /locus_tag="ACHWN2_003758"
     CDS             complement(<1..170)
                     /locus_tag="ACHWN2_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933107.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="ACHWN2_003759"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="ACHWN2_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR8933108.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="ACHWN2_003760"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="ACHWN2_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHWN2_003761"
     CDS             complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHWN2_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR8933109.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHWN2_003762"
                     /pseudo
     CDS             4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHWN2_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            5484..>5702
                     /locus_tag="ACHWN2_003763"
     CDS             5484..>5702
                     /locus_tag="ACHWN2_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFR8933110.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAZSNO010000204         5637 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZSNO010000204 DAZSNO010000000
VERSION     DAZSNO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.206"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(382..720)
                     /locus_tag="ACHWN2_003764"
     CDS             complement(382..720)
                     /locus_tag="ACHWN2_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933111.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            642..1769
                     /gene="ltrA"
                     /locus_tag="ACHWN2_003765"
     CDS             642..1769
                     /gene="ltrA"
                     /locus_tag="ACHWN2_003765"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR8933112.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFNGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            1852..2031
                     /locus_tag="ACHWN2_003766"
                     /pseudo
     CDS             1852..2031
                     /locus_tag="ACHWN2_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2058..2543)
                     /locus_tag="ACHWN2_003767"
     CDS             complement(2058..2543)
                     /locus_tag="ACHWN2_003767"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR8933113.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2799..3779)
                     /locus_tag="ACHWN2_003768"
     CDS             complement(2799..3779)
                     /locus_tag="ACHWN2_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR8933114.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3779..4978)
                     /locus_tag="ACHWN2_003769"
     CDS             complement(3779..4978)
                     /locus_tag="ACHWN2_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR8933115.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5152..5286)
                     /locus_tag="ACHWN2_003770"
                     /pseudo
     CDS             complement(5152..5286)
                     /locus_tag="ACHWN2_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5300..>5637)
                     /locus_tag="ACHWN2_003771"
     CDS             complement(5300..>5637)
                     /locus_tag="ACHWN2_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8933116.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1554 a         1285 c         1337 g         1461 t
ORIGIN      
        1 ggtaaaacaa ggactgcccg gctgtgggtc tacgttcgtg atgaccgtaa cgccggttcg
       61 gaaattgcag cataagttat ccatgctctg aggtgggtag aggtgggccc tccggtcagg
      121 gtttacagga gcacagacca aacgacccgg tgctgcttca ttctaaggaa tgaggtggtg
      181 agcgactcgg gaaaaggcag agatctactg tcacgtgggt tcgggaagct gaacgaaagt
      241 aaactgtccg atgaagcatc gaaatcttag atgatgccaa aatggggccg tacagccgtc
      301 ccctgataag cgcagaagac acctgcttac tgactgcgcg gcatccggtg tgaaggcagc
      361 aggatcccgg gccagaccat ctcagggaac gtgagaacct gtttccggca cgtatcggga
      421 aacaggggcg gacccattcg tagtagtgat gaaagatctg taatgggtct ggagcgaagg
      481 gatggggatc acattcattg cagaagacac aacccaaaag gggatgacgt aactgatgca
      541 acgaaaatca tttgaaatac caaaagcact ggtctgggca tcatatctgg atgtgcgtcg
      601 caacaaggga gccccccgga tgcgacgggc agactttgaa aatgttcgac cagcaacgtg
      661 atggcaatct atacaagatc tggaaccggc tatgttcggg aacatggttt ccgccaccag
      721 tgctggaaaa acggatcccg aagtcgaatg gcaaggaacg cattctgggg atcccaacag
      781 tatccgatcg aattgctcag ggagcgataa aacttttcat ggaagaaaag cttgacccga
      841 ttttccatgc tgattcatat ggttaccgac caggcaaatc agctcatgac gctctgaaac
      901 aatgtgccat ccggtgctgg cgttatagct ggatactgga agtggacatc agtgcgtttt
      961 tcgaccatgt gagacatgat ctggtgctca aagctctgga acaccacggg atgcctaaat
     1021 gggtcatcct gtactgtcga cgctggatgg aagcgccaat gcagagttgt gaaaatggag
     1081 aattaataac ccgaacacga ggcactccgc agggcggggt cattagtccg ctacttgcta
     1141 acttgttcct tcactatgca tttgatttgt ggatggaaag agaatatcgg ggggtaccgt
     1201 ttgagaggta cgctgacgat attgtagtac attgttcacg aatgagtgat gcaacaaggc
     1261 taaagaacag gttatcggag cgcttctcgg aagtcgggct ggtcctgaat gcagggaaaa
     1321 cgaatatcgc ctacattgac acgtttaaaa ggagaaacgt cgcaacgagt ttcacctttc
     1381 tcggatatga cttcaaagtg cgtacgctga agaatttcaa cggcgaacgg taccgaaaat
     1441 gcatgccggg tgcgtcaaat gcagcaatgc gcaaaataac agaaacaatc aagaagtggc
     1501 gtatacatcg ctcaacagct gagagtttgc tggattttgc gaggcgctac aatgcgatag
     1561 tgagaggctg gatcgagtac tacggaaagt tctggtccag aaatttcaac tatcgactgt
     1621 ggagtgcaat gcagtcacgt ctgctcaagt ggatgcagtc taaatacaga ctttcgaacc
     1681 ggaaggctca gcgaaagctg acgctggtaa ggaaggagta tccgaagcta tttgttcact
     1741 ggtatttact acgtgcatca aatgagtgat caagagccgt atgacgcgag agtgtcacgt
     1801 acggttccgt gagcagccgg ggagtgaaat tctctccggc tgactcgact cgcgggttcc
     1861 gacagcgatg acgaacatgc ggcggtgttg tactcgctga tcggcacatg ccgtctgaac
     1921 aatgtggaac cagaaaaatg gctgcgttac gtcattgagc atatccagga ctggccggca
     1981 aaccgggtac gtgatctgtt gccctggaaa gttgatctga gctctcagta aatatcaata
     2041 cggttctgac gagccgctta cttttggcgt aattctgctg cgcctttact gaacacaatg
     2101 ccgtattcag caagaagtcc acgaatgtga tttatcagtt gtgtcctttc cattgaaacc
     2161 aaccgacgca tattcaaaaa agtctggacg tcctgttgct ccagagtttt tattggtacg
     2221 ggtcgtatag ttccatgttg gcaggcctca gctattgcct gtgcgtcgtt ataatcattt
     2281 ttctggcacc gctgataagc tttaacatgt tgtgccggaa gcaatattac atcatggtcg
     2341 agtttcctga tttcccgggc ccagtaatga gatgccccgc cagcctccat cgcaacagtc
     2401 attttaggct gttgcaccag ccagttaagc agttgattgc gagaaagttt ggtctttcct
     2461 gcgggtttgc catgagcatt gatactgaaa agataaaagt tcgttttagc cagatcaatg
     2521 ccggtgattt tctgttcaga catggtatcc cgcttacttt tgttgttaaa gtttatccat
     2581 catggcgcaa ttgaaggctg ccgtgaaaga gggggaagac tatctcatcg gttactgtta
     2641 aacggctaag agaaactatc tttttcaagc aaagaagata cggtgaaaca ggcgatttca
     2701 ccgtgaaata ctgttcaaaa aaaacgctaa taaaagcaaa atttctttta aaacagccaa
     2761 ttaaaaactg gcgagttgct ggcgaaaaac ttgaattttc acagcgaatt cagattatct
     2821 tttagtacaa gagcaatcat acgatctaac ttatcctgaa cttccaacgg taatctgccg
     2881 aattcatatg agaacgtacg tccctttact cgttttcgag caaatttatc tttactatca
     2941 aatttccata acaaagttac aaccgcttta tcctttactc ctgaatcaag caatgaagac
     3001 atttccttgg ttataagccg cataatactg tttttaacct cgtcttcaga aagattgtca
     3061 tcagcagtaa taagctcaat actcggcgag atattcttca caacttcatc aatagatata
     3121 tcacccagcc tcagtcgttc acttaattca gccagttgcc tgtaatcggc gtatgtcagc
     3181 tcagactgaa caggaaaaag agacacaaaa tctttaggta cggacgctgc ttggagggca
     3241 cgagtcacct tagctgccga caatccctct ctttccgcaa tttgcttctg gttcataccg
     3301 gcctctttga gacgtactaa acgcaaccca atttcacgaa tattatgttc aagtgacgtc
     3361 tgcaaatctt tcgccagatg ctgagcttca gaaacagtaa gctcatcatc cgttacaagt
     3421 acacgtaatc caaccttgca tagcaaagct gctgcccgac gacgggaacc atccaaaatc
     3481 tcaataagat cgccagtccg aatcccaata catggataga attgttgtaa acgtatagtt
     3541 ctggtgatgt cttttagtga gtccggcgtc agggctgtct gttcgcggcc attcacctct
     3601 tcaaccacaa aagtttgagt atccactttg tcatgatcaa ccttgatctc agtaaatttt
     3661 gcctttcggc cagtattgag ggtaaataca tgaactggag ccgatatttc ttcagtatta
     3721 ttcaggatat ttgtattcag cgtacggcca atagtcggtc ggtgctttct gttttccatc
     3781 agtagttggc ccttataaat tcaatacgat caaaaactgc tttagcgaaa tcttctgctg
     3841 ccatacgtgc atttttcaaa gcttcaccac tgccaacata agtaacagga ttcgcagata
     3901 tcacggtatc aaacgactcg ccgcttctct caaaaccatc aagacgtggc attgaaacat
     3961 caagcatgtc accaccgaaa atctcttttg tcaggctatg gcagtattta tgatctgact
     4021 tattcgccag tttcgacatg aagccaatat ttgcctgcaa acggcatgaa catccatcct
     4081 gttcaataat ctgtacaagt tcaggtaaac gagccaaata tttaagcgtg gaatggaaat
     4141 caacttgtgc tggcggcacc ggtgtaaaca tgatatctgc ggcagcaatg gcatttttca
     4201 aaaatgcgtc cagatgcggg cctgtatcaa taaggatgaa gtcgaaatca tgcttcagct
     4261 tatcaataat attctcacgc aatatcgcgt gtttgttttg tcctaataga tgctcctcgc
     4321 acagagtatc ccagtttgat gcaataaagg cgtcgtcaat agaagccggc ataacataaa
     4381 cgccagggat aactgacgga acgataaaat cttccagtaa ttcctcacga ctcacgttct
     4441 gcaacatagc ctgcggtgcg gttgtatcaa ccagccccac cgcatgcaag tagttcagaa
     4501 acatagtggc tgatgactgg ggatctaaat caaggagcag aatacgtaaa tcttctgaca
     4561 gcaggtgagg atgagcacgc agggcgtgag ctacggaaac actactcacg gttttagaca
     4621 caccgccttt tagactaccg atgaatatgg aatatgcctc actataacga tcgcgatatt
     4681 taggaatgcc acgatgtgca tagatatcga taatgttctg gatggttaat gcatattttg
     4741 tcgcggttcc tgctgggcgt ttttcaaact gatagccctg agcctccatc tcaccgactg
     4801 caccatccac gcttgcgcgc gttaattttg gaagcttagc tacagcagac tttgaataga
     4861 cttgataaaa aacatcagcc tgaaactcct gcttttgctc ctgaatctgt tttgttaggg
     4921 cgagcaacat tttatctgca cgctgcgcaa ccttacttaa ctgctcaaaa ctagtcattc
     4981 cccctccgtg ctatgtagga ttttaactta ttacaaaaaa aactgcatta gcagggtaat
     5041 gcatgcaagt acatattgta catgcttata ttaaaaatgc gcgcattgct gatttattaa
     5101 tcatcggaat gaatattact gtaagatagt atgcctttcc gggagaaaga cgctgatttc
     5161 ccccgaataa tccagttcac caccacactc cggacaacag ctttcttcag gctccagatg
     5221 gtgtatttcg cgggaaacat gttccggtaa cggacggcga tgacgtgact gtcgcaacag
     5281 gcgggggtag tgcttccaat cagtagaaca tgtgcttttc aatgaaggag ccgatgactt
     5341 tttcgtggat ttccacggaa cgcgagaagc agattgtttt acgtgccagc cgcttgatgc
     5401 gggtgcggag ggtccggttg ttacgtgcaa tacgttgcgt gaagattttg ccggtcaggt
     5461 gtttctcctt cggcacttct ctggcgtagc tgccccagtc atcgctggtc accatgccga
     5521 tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc cgtggcccga
     5581 aagtgtaagc caacacaccg ccagttttag tgttgtacgc ataccagagc cagtgtt
//
LOCUS       DAZSNO010000205         5613 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZSNO010000205 DAZSNO010000000
VERSION     DAZSNO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.207"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(2..298)
                     /locus_tag="ACHWN2_003772"
     CDS             complement(2..298)
                     /locus_tag="ACHWN2_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR8933117.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="ACHWN2_003773"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="ACHWN2_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR8933118.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACHWN2_003774"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACHWN2_003774"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR8933119.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="ACHWN2_003775"
     CDS             complement(1784..2323)
                     /locus_tag="ACHWN2_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR8933120.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="ACHWN2_003776"
     CDS             complement(2353..3096)
                     /locus_tag="ACHWN2_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR8933121.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="ACHWN2_003777"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="ACHWN2_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3453..4091
                     /gene="ompW"
                     /locus_tag="ACHWN2_003778"
     CDS             3453..4091
                     /gene="ompW"
                     /locus_tag="ACHWN2_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR8933122.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4151..4657)
                     /locus_tag="ACHWN2_003779"
     CDS             complement(4151..4657)
                     /locus_tag="ACHWN2_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR8933123.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4703..5203)
                     /locus_tag="ACHWN2_003780"
     CDS             complement(4703..5203)
                     /locus_tag="ACHWN2_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR8933124.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5289..5468)
                     /locus_tag="ACHWN2_003781"
     CDS             complement(5289..5468)
                     /locus_tag="ACHWN2_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR8933125.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         1531 a         1311 c         1293 g         1478 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaagc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa
     3361 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     3421 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     3481 cagtaacaac tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     3541 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     3601 gtggattcag cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg
     3661 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     3721 cgaccggcga tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt
     3781 ttggtgatgc cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct
     3841 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     3901 aagattcctg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     3961 tggttaacat gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
     4021 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     4081 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     4141 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     4201 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     4261 tcgcttcgat gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     4321 aggtgtaaca ggtgatttca aattgctcga agacatatcc gccaatagag cctttaacta
     4381 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     4441 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     4501 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     4561 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     4621 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     4681 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     4741 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     4801 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     4861 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     4921 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     4981 atttcatgcg cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa
     5041 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     5101 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     5161 aaagcagttg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     5221 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     5281 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     5341 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     5401 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaact accacgatgt
     5461 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     5521 gacactcatt aaaattagtc actaatgaga attagtcaaa ttaagcgcaa cgagaagata
     5581 gagggaaata tattttgagg aacattctgg ata
//
LOCUS       DAZSNO010000206         5599 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZSNO010000206 DAZSNO010000000
VERSION     DAZSNO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.208"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..410
                     /locus_tag="ACHWN2_003782"
     CDS             <1..410
                     /locus_tag="ACHWN2_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8933126.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /gene="istB"
                     /locus_tag="ACHWN2_003783"
                     /pseudo
     CDS             442..1056
                     /gene="istB"
                     /locus_tag="ACHWN2_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="ACHWN2_003784"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="ACHWN2_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFR8933127.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1331 g         1488 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tctacagcgc aggagagctg
      721 ttgcaggaac tacgtaaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag acctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggcata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggac ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gaccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc ggcgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaggaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tactcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatc
//
LOCUS       DAZSNO010000207         5591 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZSNO010000207 DAZSNO010000000
VERSION     DAZSNO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.209"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..279)
                     /locus_tag="ACHWN2_003785"
     CDS             complement(1..279)
                     /locus_tag="ACHWN2_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933128.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHWN2_003786"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHWN2_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR8933129.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="ACHWN2_003787"
     CDS             complement(1254..1670)
                     /locus_tag="ACHWN2_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933130.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="ACHWN2_003788"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="ACHWN2_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2887
                     /locus_tag="ACHWN2_003789"
                     /pseudo
     CDS             1837..2887
                     /locus_tag="ACHWN2_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2915..3004)
                     /locus_tag="ACHWN2_003790"
     tRNA            complement(2915..3004)
                     /locus_tag="ACHWN2_003790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHWN2_003791"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHWN2_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR8933131.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="ACHWN2_003792"
     tRNA            3996..4071
                     /locus_tag="ACHWN2_003792"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="ACHWN2_003793"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="ACHWN2_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1509 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct a
//
LOCUS       DAZSNO010000208         5580 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZSNO010000208 DAZSNO010000000
VERSION     DAZSNO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.210"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..242
                     /locus_tag="ACHWN2_003794"
     CDS             <1..242
                     /locus_tag="ACHWN2_003794"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFR8933132.1"
                     /translation="LLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLH
                     AKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            235..621
                     /locus_tag="ACHWN2_003795"
     CDS             235..621
                     /locus_tag="ACHWN2_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HFR8933133.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            629..1315
                     /locus_tag="ACHWN2_003796"
     CDS             629..1315
                     /locus_tag="ACHWN2_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR8933134.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1293..1916)
                     /gene="tetR(B)"
                     /locus_tag="ACHWN2_003797"
     CDS             complement(1293..1916)
                     /gene="tetR(B)"
                     /locus_tag="ACHWN2_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR8933135.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1998..3203
                     /gene="tet(B)"
                     /locus_tag="ACHWN2_003798"
     CDS             1998..3203
                     /gene="tet(B)"
                     /locus_tag="ACHWN2_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR8933136.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3316..3909)
                     /gene="tetC"
                     /locus_tag="ACHWN2_003799"
     CDS             complement(3316..3909)
                     /gene="tetC"
                     /locus_tag="ACHWN2_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFR8933137.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3997..4413
                     /locus_tag="ACHWN2_003800"
     CDS             3997..4413
                     /locus_tag="ACHWN2_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8933138.1"
                     /translation="MYIEQHSRYQNKANNIQLRYDDKQFHTTVIKDVLLWIEHNLDQS
                     LLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALK
                     YQFDSQQSFTRRFKYIFKVTPSYYRRNKLWELEAMH"
     gene            complement(4423..>5580)
                     /locus_tag="ACHWN2_003801"
     CDS             complement(4423..>5580)
                     /locus_tag="ACHWN2_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HFR8933139.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1542 a         1029 c         1215 g         1794 t
ORIGIN      
        1 cactattaag tcatgtagct ggttttattt caattgaacg ttttcaaagt ctagctacag
       61 aaggaaaaat gttatcgcta tcttggtggg aaaacgaata cgcagttctg caatggaaaa
      121 atcatgtttt acatgcgaaa gctcaacaag aagggcgaga gtcaatattt gatttttaca
      181 aaattagtat tgctcatatt actcgcgaat attcatttaa aaaggacaag gataatgttt
      241 gatgttcacg ttgttttaga taatcaaata ggacaattag cattactagg aaaaacatta
      301 ggtaataaag gtattggatt ggaaggggga gggatattta cggttggtga tgaatgccat
      361 gctcattttc ttgttgaaca aggaaaggaa gctaaaatag cgctagagca agctggactg
      421 ttagtacttg cgatccggac accattaatt cgtaagttaa aacaggaaaa accgggggaa
      481 cttggcgaaa tagcacgagt attggcggag aataacatta atattttagt gcaatacagt
      541 gaccatgcta accaactgat attaataacg gacaatgata gtatggctgc atctgttacg
      601 ctcccttggg caataaagtg aacttgcgat ggctaattta atacgaaaag aggttacctt
      661 tgagtcctca atagccgcga taggggcggc tatgtctgac atttcacgag ttaaaatact
      721 cagtgctttg atggatgggc gagcttggac ggccactgag ctaagttctg tggcgaatat
      781 atcagcttca acggcgagca gtcatttatc taaattatta gattgccagc taatcacagt
      841 agtagctcaa ggcaagcatc gttattttcg gctagcagga aaagatattg ctgaattgat
      901 ggaaagtatg atggggatct ccttaaacca tggcgtacat gccaaagttt ccacgccagt
      961 gcatttacga aaagcacgta cttgctatga tcatttagct ggcgaagttg ccgttaagat
     1021 ctatgattcc ctttgtcaac agcaatggat cactgaaaat ggttcaatga tcacattaag
     1081 tggtattcaa tattttcatg aaatgggaat tgacgttcct tccaaacatt cacgtaaaat
     1141 ctgttgtgcg tgtttagatt ggagtgaacg ccgtttccat ttaggtgggt acgttggagc
     1201 cgcattattt tcgctttatg aatctaaagg gtggttaact cgacatcttg gttaccgtga
     1261 agttaccatc acggaaaaag gttatgctgc ttttaagacc cactttcaca tttaagttgt
     1321 ttttctaatc cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg
     1381 tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt
     1441 tccctttctt ctttagcgac ttgatgctct tgatcttcca atacgcaacc taaagtaaaa
     1501 tgccccacag cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa
     1561 aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt
     1621 acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt
     1681 aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac
     1741 atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca atacaatgta
     1801 ggctgctcta cacctagctt ctgggcgagt ttacgggttg ttaaaccttc gattccgacc
     1861 tcattaagca gctctaatgc gctgttaatc actttacttt tatctaatct agacatcatt
     1921 aattcctaat ttttgttgac actctatcat tgatagagtt attttaccac tccctatcag
     1981 tgatagagaa aagtgaaatg aatagttcga caaagatcgc attggtaatt acgttactcg
     2041 atgccatggg gattggcctt atcatgccag tcttgccaac gttattacgt gaatttattg
     2101 cttcggaaga tatcgctaac cactttggcg tattgcttgc actttatgcg ttaatgcagg
     2161 ttatctttgc tccttggctt ggaaaaatgt ctgaccgatt tggtcggcgc ccagtgctgt
     2221 tgttgtcatt aataggcgca tcgctggatt acttattgct ggctttttca agtgcgcttt
     2281 ggatgctgta tttaggccgt ttgctttcag ggatcacagg agctactggg gctgtcgcgg
     2341 catcggtcat tgccgatacc acctcagctt ctcaacgcgt gaagtggttc ggttggttag
     2401 gggcaagttt tgggcttggt ttaatagcgg ggcctattat tggtggtttt gcaggagaga
     2461 tttcaccgca tagtcccttt tttatcgctg cgttgctaaa tattgtcgct ttccttgtgg
     2521 ttatgttttg gttccgtgaa accaaaaata cacgtgataa tacagatacc gaagtagggg
     2581 ttgagacgca atcgaattcg gtatacatca ctttatttaa aacgatgccc attttgttga
     2641 ttatttattt ttcagcgcaa ttgataggcc aaattcccgc aacggtgtgg gtgctattta
     2701 ccgaaaatcg ttttggatgg aatagcatga tggttggctt ttcattagcg ggtcttggtc
     2761 ttttacactc agtattccaa gcctttgtgg caggaagaat agccactaaa tggggcgaaa
     2821 aaacggcagt actgctcgga tttattgcag atagtagtgc atttgccttt ttagcgttta
     2881 tatctgaagg ttggttagtt ttccctgttt taattttatt ggctggtggt gggatcgctt
     2941 tacctgcatt acagggagtg atgtctatcc aaacaaagag tcatcagcaa ggtgctttac
     3001 agggattatt ggtgagcctt accaatgcaa ccggtgttat tggcccatta ctgtttgctg
     3061 ttatttataa tcattcacta ccaatttggg atggctggat ttggattatt ggtttagcgt
     3121 tttactgtat tattatcctg ctatcgatga ccttcatgtt aacccctcaa gctcagggga
     3181 gtaaacagga gacaagtgct tagttatttc gtcaccaaat gatgttattc cgcgaaatat
     3241 aatgaccctc ttgataaccc aagagggcat tttttacgat aaagaagatt tagcttcaaa
     3301 taaaacctat ctattttatt tatctttcaa gctcaataaa aagccgcggt aaatagcaat
     3361 aaattggcct tttttatcgg caagctcttt taggtttttc gcatgtattg cgatatgcat
     3421 aaaccagcca ttgagtaagt ttttaagcac atcatcatca taagctttaa gttggttctc
     3481 ttggatcaat ttgctgacaa tggcgtttac cttaccagta atgtattcaa ggctaatttt
     3541 ttcaagttca ttccaaccaa tgataggcat cacttcttgg atagggataa ggtttttatt
     3601 attatcaata atataatcaa gataatgttc aaatatactt tctaaggcag accaaccatt
     3661 tgttaaatca gtttttgttg tgatgtaggc atcaatcata attaattgct gcttataaca
     3721 ggcactgagt aattgttttt tatttttaaa gtgatgataa aaggcacctt tggtcaccaa
     3781 cgcttttccc gagatctcat ctattgaaac agcttgatag cctttttcaa caaacaatat
     3841 tcgtgctgag ttaaccagtg attgataggt actcttaaaa ttttcttgtt gatgattttt
     3901 attttccatg atagatttaa aataacatac cgtcagtatg tttatggtat catgatgatg
     3961 tggtcgtgac aatcttaaga acatttaggt tattttatgt atattgaaca gcattctcgc
     4021 tatcaaaata aagctaataa catccaatta agatatgatg ataagcagtt tcatacaacg
     4081 gttatcaaag atgttctatt atggattgaa cataatttag atcagtcttt actgcttgat
     4141 gatgtggcga ataaagcggg ttataccaag tggtattttc agcggctgtt caaaaaagta
     4201 acaggggtca cactggctag ctatattcgt gctcgtcgtt tgacgaaagc ggctgttgag
     4261 ttgaggttga cgaaaaaaac tatccttgag atcgcattaa aatatcaatt tgattcccaa
     4321 caatctttta cacgtcgatt taagtacatt tttaaggtta caccaagtta ttatcggcgt
     4381 aataaattat gggaattgga ggcaatgcac tgagagatcc cctcataatt tccccaaagc
     4441 gtaaccatgt gtgaataaat tttgagctag tagggttgca gccacgagta agtcttccct
     4501 tgttattgtg tagccagaat gccgcaaaac ttccatgcct aagcgaactg ttgagagtac
     4561 gtttcgattt ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt tctgagcatg
     4621 aacgcccgca agccaacatg ttagttgaag catcagggcg attagcagca tgatatcaaa
     4681 acgctctgag ctgctcgttc ggctatggcg taggcctagt ccgtaggcag gacttttcaa
     4741 gtctcggaag gtttcttcaa tctgcattcg cttcgaatag atattaacaa gttgtttggg
     4801 tgttcgaatt tcaacaggta agttagttgc tagaacccat ggctcctttg ccgacgctga
     4861 gtagatttta ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat tttttcggcc
     4921 tttagagcga gatttataca atagaatttg gcatgagatt ggattgcttt tagtcagcct
     4981 cttatagcct aaagtctttg agtgactaga tgacatatca tgtaagttgc tgataggttt
     5041 ccagttttcc gctcctaggt ctgcatattg tacttttcct cttactcgac ttaaccagta
     5101 ccaacccagc ttctcaacgg atttatacca tggcacttta aagccagcat cactgacaat
     5161 gagcggtgtg gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt ggtcatgagc
     5221 tttctttgaa cattgctctg aaagcgggaa cgctttctca taaagagtaa cagaacgacc
     5281 gtgtagtgcg actgaagctc gcaataccat aagtcgtttt tgctcacgaa tatcagacca
     5341 gtcaacaagt acaatgggca tcgtattgcc cgaacagata aagctagcat gccaacggta
     5401 tacagcgagt cgctctttgt ggaggtgacg attacctaac aatcggtcga ttcgtttgat
     5461 gttatgtttt gttctcgctt tggttggcag gttacggcca agttcggtaa gagtgagagt
     5521 tttacagtca agtaatgcgt ggcaagccaa cgttaagctg ttgagtcgtt ttaagtgtaa
//
LOCUS       DAZSNO010000209         5557 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZSNO010000209 DAZSNO010000000
VERSION     DAZSNO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.211"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..641
                     /locus_tag="ACHWN2_003802"
     CDS             <1..641
                     /locus_tag="ACHWN2_003802"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HFR8933140.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="ACHWN2_003803"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="ACHWN2_003803"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR8933141.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="ACHWN2_003804"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="ACHWN2_003804"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR8933142.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1376 a         1360 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAZSNO010000210         5546 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZSNO010000210 DAZSNO010000000
VERSION     DAZSNO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.212"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..407
                     /locus_tag="ACHWN2_003805"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHWN2_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..4690
                     /gene="pic"
                     /locus_tag="ACHWN2_003806"
     CDS             572..4690
                     /gene="pic"
                     /locus_tag="ACHWN2_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFR8933143.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4841..5083)
                     /locus_tag="ACHWN2_003807"
                     /pseudo
     CDS             complement(4841..5083)
                     /locus_tag="ACHWN2_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5122..>5546)
                     /locus_tag="ACHWN2_003808"
     CDS             complement(5122..>5546)
                     /locus_tag="ACHWN2_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8933144.1"
                     /translation="EQRLAARKARAAPLMQSLYDRIQQQMKTLSRHSDTAKAFAYLLK
                     QWKALNVYCSNGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCK
                     LNDVDPESYLRHVLGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT         1457 a         1287 c         1482 g         1320 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgtccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgggta gtggtacgca gtacactaag gacaaggacg gaaatttagt
     1201 taaggttgcc ggtggatatg cttttaaaac aggaggaacc acaggagttc ctctgatatc
     1261 tgatgcaaca atagtctcta atcccgggca aacttataat cctgtaaacg gccctttacc
     1321 tgactatgga gcccctgggg atagtggttc tcctttgttt gcttatgata aacaacaaaa
     1381 aaaatgggtt attgttgctg tattaagagc atatgcaggt attaatggtg ctacgaactg
     1441 gtggaatgtc ataccaacag attatctgaa ccaggttatg caggacgatt tcgatgcccc
     1501 cgtagacttt gtttccggac tgggccccct gaactggaca tacgacaaaa catcaggcac
     1561 aggtaccctg agccagggca gtaaaaactg gaccatgcac gggcagaaag acaatgacct
     1621 caatgccggt aaaaatctgg tattcagcgg gcagaatggt gcaattatcc tgaaagacag
     1681 tgtgactcag ggtgccggtt atctcgaatt taaagacagt tacaccgtat ctgctgaatc
     1741 cggaaaaaca tggacgggtg ccggcattat tactgacaag gggacgaatg taacctggaa
     1801 ggtcaacggc gttgccggtg acaacttgca taagctgggg gaaggaaccc tgaccataaa
     1861 cggaacaggt gtaaacccgg gaggactgaa aacgggagac ggtatcgttg tacttaacca
     1921 gcaggcagac actgcaggta atatccaggc cttcagttca gtgaacctcg ccagcggacg
     1981 tccgaccgtg gtgctcgggg atgcccgtca ggtcaatccg gataacattt catggggata
     2041 ccggggaggt aagcttgacc ttaatggtaa tgccgttacc ttcacccgac tgcaggctgc
     2101 tgattacggg gcggtgatta caaataatgc acagcaaaaa tcccagcttt tactggatct
     2161 taaggctcag gatacaaatg tcagtgaacc gacgattgga aatatatccc cctttggtgg
     2221 taccggcact ccaggaaacc tgtacagcat gatactcaac agccagaccc gcttctatat
     2281 tctgaaatct gccagctatg gtaacactct gtgggggaac agcctgaatg atccggctca
     2341 gtgggagttt gttggcatga acaaaaacaa agcagttcag acagtaaaag ataggatcct
     2401 ggccgggcgg gcaaaacaac ccgttatctt tcatggtcag ctgaccggga atatggatgt
     2461 cgccattcca caggtgccgg ggggaagaaa ggtaatcttt gatggtagcg tgaacctgcc
     2521 ggaaggtacc ctgagtcagg acagtggcac cctgatattc cagggacatc cggttatcca
     2581 tgcctccatc agtggcagtg caccggtcag cctgaaccag aaagactggg aaaaccgtca
     2641 gtttacaatg aaaacactgt cgctgaaaga cgctgacttc catctttcac gtaacgcctc
     2701 gctgaacagt gacattaagt cggataacag ccatatcaca ctgggaagtg acagggcatt
     2761 tgtggataaa aatgacggaa caggaaatta tgtcattccg gaggaaggta cctctgtccc
     2821 ggacaccgtg aatgacagga gccagtatga agggaatatt acgctgaacc ataactcagc
     2881 cctggatatc ggcagcaggt tcaccggggg gattgacgct tatgacagtg ccgtcagcat
     2941 cacctctccg gacgtcctgt tgacagcccc gggtgctttt gccggcagtt cactgacagt
     3001 gcatgatggc ggtcatctta cagcactgaa cggtcttttc agcgacgggc atattcaggc
     3061 cggtaagaac ggcaaaatca ccctgagcgg tacaccggtt aaagatacgg ctaatcagta
     3121 tgcccctgct gtatatctga cggacggata tgacctgacc ggcgataacg cagcactgga
     3181 aattacccgt ggagcacatg cttccggtga tattcatgcc tctgcggcat caacagttac
     3241 catcgggtct gacacgccgg cagaactggc ttctgcggaa acggctgcat cggcgtttgc
     3301 cggcagtctt cttgagggct ataacgcagc attcaatggt gccataaccg gtggcagggc
     3361 tgatgtcagt atgcataatg cactgtggac tctgggtggg gactctgcca tccacagtct
     3421 taccgtcaga aacagccgta ttagttctga aggagaccgt acattccgta ccctgacggt
     3481 gaataaactg gatgcaacag gcagtgattt tgttttgcgt acggacctga aaaatgccga
     3541 taaaattaat gtgactgaaa aagccactgg ttcagataac agcctgaacg tcagctttat
     3601 gaataatcct gctcagggac aggccctgaa tattcctctg gtcacggcac cggcgggaac
     3661 ttcagcagag atgtttaagg ccggcacccg ggtgacaggt ttcagtcggg tgaccccaac
     3721 cctgcatgtt gataccagtg gtggcaatac gaagtggata ctggatggtt ttaaagcgga
     3781 ggctgataaa gccgctgccg cgaaggctga cagtttcatg aatgccgggt ataaaaactt
     3841 catgacggaa gttaacaatc tgaacaaacg tatgggtgac ctgcgtgaca caaacggtga
     3901 tgccggtgcc tgggcgcgca tcatgagtgg tgccggttct gcagacggtg gttacagtga
     3961 taattacacc catgttcagg tcggctttga caaaaaacat gaactggacg gtgtggacct
     4021 gtttaccggt gtcacgatga cctataccga cagcagtgca gacagccatg cattcagcgg
     4081 aaagacgaaa tcggtggggg gcggtctgta tgcttcagca ttgtttgagt ccggtgccta
     4141 tatcgatttg attggtaaat atattcacca tgacaatgat tacacaggta actttgctag
     4201 cctgggaacg aaacactaca acacccattc ctggtatgcc ggtgctgaaa cgggttaccg
     4261 ctatcacctg acagaggaca cgttcattga gccgcaggct gaactggttt acggcgccgt
     4321 gtccgggaaa acattccgct ggaaagacgg tgatatggac ctgagcatga agaacaggga
     4381 cttcagtccg ctggttggaa gaacaggggt tgaactgggc aagaccttca gtggtaagga
     4441 ctggagtgtg acggcccgtg ccggaaccag ctggcagttt gacctgctga ataatggaga
     4501 gaccgtactg cgtgatgcgt ccggggagaa acggataaaa ggagagaagg acagccggat
     4561 gctgtttaat gttggtatga atgcgcagat aaaggacaat atgcgctttg gtctggagtt
     4621 tgagaagtca gcctttggta aatataacgt ggataatgcg gtaaacgcga atttccggta
     4681 tatgttctga tgtgggggta aagggctgcc ggaaggcagc ctttctcaga gactcttaaa
     4741 atataaaact gaaggtcttc ggtaaggggg agtgtattag acgtcaacta gttttgccga
     4801 ctggcgcgag cctgtttaag attctgtgta aatgcctttc tcagaagtga ccgtccaggc
     4861 ggtcaccgaa ctcgataata aagcggctca ttgccattcg ccagtccttc aacggcatcg
     4921 tccatttctg ggacgcagac tggattgcca gccacaccac ttttttcacc gagtcgtctg
     4981 tcgggaacac tttacgcttt ttgatcgcat ggcggatcac gctgtttagc gactcgatgg
     5041 cattcgtcgt atagatcact ttgcggatgt ccgttggata agcggtaagc gtacagcgag
     5101 ggccgtattg acggggatgt gttattcagc tggcagtgct atgcgccacg gaagcagttc
     5161 gctgacccgg ttgaccggcc agtctgctat gacgccaagc acatggcgaa ggtagctttc
     5221 tggatccacg tcattcagtt tgcacgtccc gatcaggctg tacagtagcg cgccccgctc
     5281 accaccatgg tcagagccga agaacaggaa gtttttacga cccagactga ccgcccgcag
     5341 ggcattttca gcgatgttgt tgtcgatttc cacccagcca ttactgcagt acacgttcag
     5401 ggccttccac tgtttcagta ggtatgcgaa cgcttttgcc gtatccgagt gacgcgacag
     5461 tgttttcatc tgttgctgta tccggtcata cagtgactgc atcagtggcg cggctcttgc
     5521 ttttcttgcc gccagacgct gttctg
//
LOCUS       DAZSNO010000211         5522 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZSNO010000211 DAZSNO010000000
VERSION     DAZSNO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.213"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            564..1553
                     /gene="araF"
                     /locus_tag="ACHWN2_003809"
                     /pseudo
     CDS             564..1553
                     /gene="araF"
                     /locus_tag="ACHWN2_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            1623..3137
                     /gene="araG"
                     /locus_tag="ACHWN2_003810"
     CDS             1623..3137
                     /gene="araG"
                     /locus_tag="ACHWN2_003810"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR8933145.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3152..4138
                     /gene="araH"
                     /locus_tag="ACHWN2_003811"
     CDS             3152..4138
                     /gene="araH"
                     /locus_tag="ACHWN2_003811"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR8933146.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4305..5105
                     /gene="otsB"
                     /locus_tag="ACHWN2_003812"
     CDS             4305..5105
                     /gene="otsB"
                     /locus_tag="ACHWN2_003812"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR8933147.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            5080..5522
                     /locus_tag="ACHWN2_003813"
                     /pseudo
     CDS             5080..5522
                     /locus_tag="ACHWN2_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
BASE COUNT         1363 a         1271 c         1476 g         1412 t
ORIGIN      
        1 atgatagtgc tttttgtatc ttgttgatat taatcgatga atttgaattc atgatgaagt
       61 gatgatttta taaaacgttt tttcattttt gcgagatggc tctcattata cgtgttctgt
      121 taattaatga gcacagtgat aattaatggc gaaatgaatg acgcgcattt tccacatctt
      181 tgagttgtgg ttattacacc atttcaaaaa ctcaacgcca ggtaatgcgg cctattgact
      241 ggttaaaaag aagacatccc gcatgggtac cgaagacaac aaggatttcc aggctaatct
      301 tatggattaa tctgctgtgc attcaacaat ttgtctgaca aattggcttt tccttatgtc
      361 ttttcccgct aaatttatgc acgttctcac tgtaattctg cgatgtgata ttgctctcct
      421 atggagaatt aatttctcgc taaaactatg tcaacacggt cacttatctt ttagataaaa
      481 ggtaatgctt tgttttccga ttaatttaac gaatgtcatt cgtttttgcc ctacacaaaa
      541 cgacactaaa gctggagaga accatgcaca aatttactaa agccctggca gccattggtc
      601 tggcagccgt tatgtcacaa tccgctatgg cggagaacct gaagctcggt tttctggtga
      661 agcaaccgga agagccgtgg ttccagaccg aatggaagtt tgccgataaa gccgggaagg
      721 atttagggtt tgaggttatt aagattgccg tgccggatgg cgaaaaaaca ttgaacgcga
      781 tcgacagcct ggcagccagt ggcgcaaaag gtttcgttat ttgtactccg gacccgaaac
      841 tcggctctgc catcgtcgcg aaagcgcgtg gctacgatat gaaagtcatt gcagtggatg
      901 accagtttgt taacgccaaa ggtaagccaa tggataccgt tcctctggtg atgatggcgg
      961 cgactaaaat tggcgaacgt cagggctagg aactgtataa agagatgcag aaacgtggct
     1021 gggatgtcaa agaaagcgca gtgatggcga ttaccgccaa cgaactggat accgcccgcc
     1081 gccgtacaac gggttctatg gacgcactga aagctgcagg attcccggaa aaacaaattt
     1141 atcaggtacc taccaaatct aacgacatcc cgggggcatt tgacgctgcc aactcaatgc
     1201 tggttcaaca tccggaagtt aaacattggc tgatcgtcgg tatgaacgac agcaccgtac
     1261 ttggcggcgt acgtgcaacc gaaggtcagg gctttaaagc agccgatatc atcggtattg
     1321 gtattaacgg tgtggatgca gtaagcgaac tgtctaaagc acaggcaacc ggcttctacg
     1381 gttccctgct gccaagcccg gacgtacatg gctataaatc cagcgaaatg ctttacaact
     1441 gggtagcaaa agacgttgaa acgccaaaat ttaccgaagt tacagacgtg gtactgatca
     1501 cgcgtgacaa ctttaaagaa gaactggaga aaaaaggttt aggcggtaag taatttgccg
     1561 gaaaaattcc cctctgcatg atgcagaggg ggtgtgaacg accagtgatt cacggagacg
     1621 ttatgcaaca gtctaccccg tatctctcat ttcgcggcat cggtaaaacg tttcccggcg
     1681 ttaaggcgct gacggatatt agttttgact gctatgccgg tcaggttcat gcgttgatgg
     1741 gtgaaaatgg cgcaggaaaa tcaactctct taaaaatcct cagcggcaac tatgcgccaa
     1801 ccacgggttc tgtagtgatt aatgggcagg aaatgtcctt ttccgacacg accgcagcac
     1861 ttaacgcggg tgtggcgatt atttaccagg aactgcatct cgtgccggaa atgaccgtcg
     1921 cggaaaacat ctatctcggc cagctgccgc ataaaggcgg cattgtgaat cgctcattgc
     1981 tgaattatga ggcgggttta caacttaaac atcttggtat ggatattgac ccggacacgc
     2041 cgctgaaata tctcgccatt ggtcagtggc agatggttga aatcgccaaa gcgctggcgc
     2101 gtaacgctaa aattatcgcc tttgatgagc caaccagctc cctttctgcc cgcgaaatcg
     2161 gcaatctttt ccgcgttatt cgtgaactgc gaaaagaggg gcgggtgatc ttatacgttt
     2221 ctcaccgtat ggaagaaata tttgccctca gcgatgccat cactgtcttt aaagatggac
     2281 gttatgtcaa aacctttacc gatatgcagc aggttgacca cgacgcgctg gtgcaggcga
     2341 tggtcgggcg cgacattggc gatatctacg gctggcaacc tcgtagttat ggcgaggagc
     2401 gcctgcgtct tgatgctgtg aaagcactag gcgtgcgtac gccaataagt ctggcggttc
     2461 gcagtggtga aattgttggg ctgtttggtc tggtaggggc ggggcgtagc gaattaatga
     2521 aaggcttgtt tggcgggacg caaatcaccg ccggtcaggt ttatatcgac caacagccga
     2581 tcgatattcg taaaccgagc cacgccattg ccgcaggcat gatgctctgc ccggaagctc
     2641 gcaaagcgga aggcattatt cccgtgcact ccgttcgcga caatatcaac atcagtgcca
     2701 gacgcaaaca tgtgctcggc ggttgtgtaa tcaacaacag ttgggaagaa aacaatgccg
     2761 atcagcacat tcgttcgctc aacatcaaaa cgccgggcgc ggagcaactg atcatgaatc
     2821 tctcaggcgg aaatcagcaa aaagccattc tgggccgctg gttatcggaa gagatgaagg
     2881 tcattttgct ggatgaacct acgcgcggca ttgatgttgg cgctaagcac gaaatatata
     2941 acgtaattta tgcgctggcg gcgcagggcg tggcggtgct gtttgcctcc agcgacttac
     3001 ctgaagtcct cggcgttgcc gaccggattg tggtgatgcg ggaaggcgaa atcgccggtg
     3061 aattgttaca cgagcaggca gatgagcgtc aggcactgag ccttgcgatg cctaaagtca
     3121 gccaggcagt tgcctgagta aggagagtat gatgtcttct gtttctacat cggggtctgg
     3181 cgcacctaag tcgtcattca gcttcgggcg tatctgggat cagtacggca tgctggtggt
     3241 gtttgcggtg ctctttatcg cctgtgccat ttttgtccca aattttgcca ccttcattaa
     3301 tatgaaaggg ttgggcctgg caatttccat gtcggggatg gtggcgtgtg gcatgttgtt
     3361 ctgccttgct tccggtgact ttgacctttc tgtcgcctcc gtaattgcct gtgcgggtgt
     3421 caccacggcg gtggttatca acctgactga aagcctgtgg attggcgtgg cagcgggttt
     3481 gttgctgggc gttctctgtg gcctggtcaa tggctttgtt atcgccaaac tgaaaataaa
     3541 tgctctgatc acgacactgg caacgatgca gattgttcga ggtctggcgt acatcatttc
     3601 agacggtaaa gcggtcggta tcgaagatga aagcttcttt gcccttggtt acgctaactg
     3661 gttcggtctg cctgcgccaa tctggctcac cgtcgcgtgt ctgattatct ttggtttgct
     3721 gctgaataaa accacctttg gtcgtaatac cctggcgatt ggcgggaacg aagaggctgc
     3781 gcgtctggcg ggtgtaccgg ttgttcgcac caaaattatt atctttgttc tctctggcct
     3841 ggtatctgcg atagccggaa ttattctggc ttcacgtatg actagtgggc agccaatgac
     3901 gtcgattggt tatgagctta ttgttatctc cgcctgcgtt ttaggtggcg tttctctgaa
     3961 aggtggcatc ggaaaaatct catatgtggt ggcgggtatc ttaattttag gcaccgtgga
     4021 aaacgccatg aacctgctta atatttctcc tttcgcgcag tacgtggttc gcggcttaat
     4081 cctgctggca gcggtgatct tcgaccgtta caagcaaaaa gcgaagcgca ctgtctgatg
     4141 cttttttcca caacaactta acgttttttc ccaccacagc cagccgccac aacggttggc
     4201 tgttcttcgt tgcaaatggc gacctccgtc acactgtcta tacttacatg tctgtaaagc
     4261 gcggtctgcg caacacaata agaaaagaga aggaggagag ccgggtgaca gaaccgttaa
     4321 ccgaaacccc tgaactatcc gcgaaatatg cctggttttt tgatcttgat ggaacgctgg
     4381 cggaaatcaa accgcatccc gatcaggtcg tcgtgcctga caatattctg caaggactac
     4441 agctactggc aaccgcaagt gatggtgcat tggcattgat atcagggcgc tcaatggtgg
     4501 agcttgacgc actggcaaaa ccttatcgct tcccgttagc gggcgtgcat ggggcggagc
     4561 gccgtgacat caatggtaaa acacatatcg ttcatctgcc ggatgcgatt gcgcgtgata
     4621 ttagcgtgca actgcatacg gtcatcgctc agtatcccgg cgcggagctg gaggcgaaag
     4681 ggatggcttt tgcgctgcat tatcgtcagg ctccgcagca tgaagacgca ttaatgacat
     4741 tagcgcaacg tattactcag atctggccac aaatggcgtt acagcaggga aagtgtgttg
     4801 tcgagatcaa accgagaggt accagtaaag gtgaagcgat tgcagccttt atgcaggaag
     4861 ctccttttat cgggcgaacg cccgtatttc tgggcgatga tttaaccgat gaatctggct
     4921 tcgcagtcgt taaccgactg ggcggaatgt cagtaaaaat tggcacaggt gcaactcagg
     4981 catcatggcg actggcgggt gtgccggatg tctggagctg gcttgaaatg ataaccgccg
     5041 cattacaaca aaaaagagaa aataacagga gtgatgacta tgagtcgttt agtcgtagta
     5101 tctaaccgga ttgcaccacc agacgagcac gccgccagtg ccggtggcct tgccgttggc
     5161 atactggggg cactgaaagc cgcaggcgga ctgtggtttg gctggagtgg tgaaacaggg
     5221 aatgaggatc agccgctaaa aaaggtaaca ttacgtgggc ctcttttaac ctcagcgaac
     5281 aggaccttga cgaatactac aaccaattct ccaatgccgt tctctggccc gcttttcatt
     5341 atcggctcga tctggtgcaa tttcagcgtc ctgcctggga cggctatcta cgcgtaaatg
     5401 cgttgctggc agataaatta ctgccgctgt tgcaagacga tgacattatc tggatccacg
     5461 attatcacct gttgcccttt gcgcatgaat tacgcaaacg tggggtgaat aatcgcattg
     5521 gt
//
LOCUS       DAZSNO010000212         5485 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZSNO010000212 DAZSNO010000000
VERSION     DAZSNO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.214"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1630)
                     /locus_tag="ACHWN2_003814"
     CDS             complement(<1..1630)
                     /locus_tag="ACHWN2_003814"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR8933148.1"
                     /translation="MENSRIPREHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2541)
                     /locus_tag="ACHWN2_003815"
                     /pseudo
     CDS             complement(1646..2541)
                     /locus_tag="ACHWN2_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(2541..3146)
                     /locus_tag="ACHWN2_003816"
     CDS             complement(2541..3146)
                     /locus_tag="ACHWN2_003816"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR8933149.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="ACHWN2_003817"
     CDS             complement(3317..>5485)
                     /locus_tag="ACHWN2_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFR8933150.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1363 c         1141 g         1400 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccta gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DAZSNO010000213         5398 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZSNO010000213 DAZSNO010000000
VERSION     DAZSNO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.215"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..497
                     /locus_tag="ACHWN2_003818"
     CDS             <1..497
                     /locus_tag="ACHWN2_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HFR8933151.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACHWN2_003819"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACHWN2_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACHWN2_003820"
     CDS             2500..3114
                     /locus_tag="ACHWN2_003820"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR8933152.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACHWN2_003821"
     CDS             3114..3443
                     /locus_tag="ACHWN2_003821"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR8933153.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHWN2_003822"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHWN2_003822"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR8933154.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="ACHWN2_003823"
     CDS             4529..4840
                     /locus_tag="ACHWN2_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR8933155.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..5373
                     /locus_tag="ACHWN2_003824"
                     /pseudo
     CDS             4824..5373
                     /locus_tag="ACHWN2_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAZSNO010000214         5364 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZSNO010000214 DAZSNO010000000
VERSION     DAZSNO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.216"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            67..2103
                     /gene="yihQ"
                     /locus_tag="ACHWN2_003825"
     CDS             67..2103
                     /gene="yihQ"
                     /locus_tag="ACHWN2_003825"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFR8933156.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            2149..3523
                     /locus_tag="ACHWN2_003826"
                     /pseudo
     CDS             2149..3523
                     /locus_tag="ACHWN2_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3566..4969
                     /locus_tag="ACHWN2_003827"
     CDS             3566..4969
                     /locus_tag="ACHWN2_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8933157.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            5037..>5364
                     /locus_tag="ACHWN2_003828"
     CDS             5037..>5364
                     /locus_tag="ACHWN2_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFR8933158.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         1264 a         1323 c         1350 g         1427 t
ORIGIN      
        1 cattttacat aagcctgtaa taacaggaag gcaacaatct cgttctcaac tctatggaga
       61 ttagttatgg atacgccacg tccacagtta ttagattttc aatttcatca gaataacgac
      121 agttttaccc tacattttca acaacgtctt attttaaccc ataacaaaga taatccttgt
      181 ttatggattg gctcaggtat agcggatatc gatatgttcc gcggtaattt cagcattaaa
      241 gataaactac aggagaaaat tgcgcttacc gacgccatcg tcagccagtc accggatggt
      301 tggttaattc atttcagccg tggttctgac attagcgcca cgctgaatat ctctgccgac
      361 gatcagggcc gtttattgct ggaactacaa aacgacaacc ttaaccacaa ccgtatctgg
      421 ctgcgccttg ccgctcaacc agaggaccat atctacggct gcggcgaaca gttttcctac
      481 ttcgatctgc gcggcaaacc gttcccgcta tggaccagtg aacaaggcgt tggtcgcaac
      541 aaacaaacct atgtcacctg gcaggccgac tgcaaagaaa atgcgggcgg cgactattac
      601 tggactttct tcccacagcc tacgtttgtc agcacgcaga agtattactg ccatgttgat
      661 aacagttgct acatgaactt cgactttagc gccccggaat accatgaact ggcgctgtgg
      721 gaagacaaag ctacgctacg ttttgaatgt gctgacacat acatctccct gctggaaaaa
      781 ttaactgccc tgctgggacg ccagccagaa ctgcccgact ggatttatga cggggtaacg
      841 ctcggcattc agggcgggac ggaagtgtgc cagaagaaac tggacaccat gcgtaacgcg
      901 ggcgtgaagg ttaacggcat ctgggcgcag gactggtctg ggatccgtat aacctctttt
      961 ggcaagcgcg tgatgtggaa ctggaagtgg aacagcgaaa actacccgca actggattca
     1021 cgcattaagc agtggtatca ggagggcgtg cagttcctgg cctatatcaa cccgtatgtt
     1081 gccagcgata aagatctctg cgaagaagcg gcacaacacg gctatctagc aaaagatgcc
     1141 tctggcggtg actatctggt ggagtttggc gagttttacg gcggcgttgt cgatctcact
     1201 aatccagaag cctacgcctg gttcaaggaa gtgatcaaaa agaacatgat tgaactcggc
     1261 tgcggcggct ggatggctga cttcggcgag tatctgccca ccgacacgta cctgcataac
     1321 ggcgtcagtg ccgaaattat gcataacgcc tggcctgcgc tgtgggcgaa gtgtaactac
     1381 gaagcccttg aagaaacggg caagctcggc gagatcctct tctttatgcg cgccggttct
     1441 accggtagcc agaaatactc caccatgatg tgggcgggcg accagaacgt ggactggagt
     1501 ctcgacgatg gcctggcgtc cgtagtcccg gcggcgctgt cgctggcaat gaccggacat
     1561 ggtctgcatc acagcgacat tggcggttac accaccctgt ttgagatgaa gcgcagcaaa
     1621 gagctgctgc tgcgctggtg cgatttcagc gccttcacgc cgatgatgcg cacccacgaa
     1681 ggtaaccgtc ctggcgacaa ctggcagttt gacggcgatg cggaaactat cgcccatttt
     1741 gcccgtatga ctaccgtttt caccaccctg aaaccttatc tgaaagaggc cgtcgcgctg
     1801 aatgcgaagt ccggtctgcc ggtcatgcgc ccgctgttcc tgcattacga agacgatgca
     1861 catacctatt ccctgaaata tcagtacctg ttaggccgcg acattctggt cgctccggta
     1921 cacgaagaag gtcgcagcga ctggacgctc tatctgccgg aggataactg ggtacatgcc
     1981 tggacgggtg aagcgttccg gggcggggaa gttaccgtta atgcgcccat cggcaagccg
     2041 ccggtctttt atcgcgccga tagcgaatgg gcggcactgt tcgcgtcgtt aaacagcatc
     2101 taatcacatc cgcccgtggg gaaactcatg ggcaaaggga gaacatcgat gagtcacatc
     2161 acaacggaag atccagcaac tttacgcctg ccctttaaag agaaactctc ttacggtatt
     2221 ggcgacctgg cctctaacat cctgctggat atcggtacgc tttatctttt gaagttttat
     2281 accgacgttc tggggctgcc aggcacctat ggcggcatta tctttttgat ttcaaaattc
     2341 tttactgcgt ttaccgatat gggtaccggc attatgttgg attcccgacg caagatcggt
     2401 ccaaaaggta agttccgtcc ttttattctg tatgcgtcat tcccggtcac cttactggcg
     2461 atcgccaact ttgtcggcac accgtttgat gtcaccggta aaacggtgat ggccactatt
     2521 ctgtttatgc tttacggact gtttttcagc atgatgaact gctcctacgg cgcaatggtc
     2581 ccggcaatca ccaaaaaccc caacgaacgc gcctcactgg cggcatggcg tcagggcggc
     2641 gctacgctgg gcctgctgct gtgcacggtg ggattcgtgc cagttatgaa tcttatcgaa
     2701 ggtaatcaga aacttggcta tatcttcgcc gccacgctgt tttcactgtt cggcctgctg
     2761 tttatgtgga tctgctactc aggcgtgaaa gagcgttatg tcgaaaccca accaaccaat
     2821 ccggcgcaaa agcctggcct gttgcaatct ttccgcgcaa ttgccggtaa ccgcccactg
     2881 ttcattctgt gtattgccaa cctctgcacc ttaggggcgt ttaacgtcaa gctcgccatt
     2941 caggtctatt acacccagta cgtacttaac gatcccatcc tgttgtcgta tatgggattt
     3001 ttcagcatgg gctgtatttt catcggcgtg ttcctgatgc ctggcgcagt cagacgtttt
     3061 ggtaagaaga aggtctatat cggcggcctg ctgatttggg tgctgggcga tctgctcaac
     3121 tatttcttcg gcggcggctc ggtcagcttt gtggcgttct cctgcctggc gttcttcggc
     3181 tcagcgcctg aactgggcgc tggtttccga caccgtcgag tacggcgagt ggcgcaccgg
     3241 cgtgcgttcg gaaggcacgg tctacaccgg cttcaccttc tttcgcaaag tgtctcaggc
     3301 gctggctggt ttcttccccg gctggatgct gacgcaaatt ggctatgtgc cgaacgtcgc
     3361 acaggctgac cacaccattg aagggttacg ccagttgatc ttcatctacc caagcgcact
     3421 ggcggtagtc accattgtgg cgatgggttg cttctacagc ctgaacgaga agatgtatgt
     3481 ccgcattgtg gaagagatag aagcccgtaa acgcacggcg taataataat aaacgacgcc
     3541 ctgcggggcg ttataaggag tgattatgtc tgaccataat ccactgacat taaaactgaa
     3601 tctgcgggaa aaaatcgcct atggtatggg cgacgtcggt tcgaatttaa tgctctgcat
     3661 cggtactctg tatctcctca aactttatac cgatgaatta gggatgcctg cttactatgg
     3721 cgggattatc tttctggtcg cgaagttttt taccgcgttt accgatatgc tcaccggatt
     3781 tttactcgac tcgcggaaaa atattgggcc taaaggcaaa tttcgtccct ttattttata
     3841 tgcagcagtt ccggcggcgt taattgcaac gctccagttt atcgccacca ccttttgttt
     3901 gccggttaaa acgacgattg ccaccacgct gtttatgatg tttggacttt catacagtct
     3961 gatgaactgc tcgtatggtg cgatgatccc ggcaattacc aaaaacccga atgagcgcgc
     4021 gcagctcgcg gcttaccgcc agggtggtgc aaccataggg ctattgattt gtaccgtggc
     4081 gtttattccg ttgcagtcgc ttttttctga ctcaaccgtc ggttatgcct gtgcggcact
     4141 tatgttctcc attggcggct ttatttttat gatgctgtgc tacagaggcg tcaaagagca
     4201 ttatgtggac acagcgccaa ccggacataa agccagtatt ctcaaatctt tttgcgcgat
     4261 atttcgtaat ccaccattgc tggttttatg cattgctaac ctgtgtactc tggcggcatt
     4321 taatatcaaa ctggcgattc aggtctatta cacccagtat gtgctgaatg atattaattt
     4381 attgtcgtgg atgggattct tcagtatggg atgcatcctc gtcggcgtat tactggtgcc
     4441 agtaactgta aaatgttttg gtaaaaaaca ggtttattta gctggcatgg tgctatgggc
     4501 ggtgggtgat atactgaatt atttctgggg aagtaactct ttcactttcg tcatgttctc
     4561 ttgtgtcgcc ttttttggca cggcctttgt aaacagcctg aactgggcac tggtaccaga
     4621 taccgtagat tacggtgaat ggaaaaccgg tattcgtgcc gaaggttctg tttataccgg
     4681 ttataccttc tttcgtaaaa tttctgccgc acttgctggc ttcttgccag gcattatgct
     4741 gacgcaaatt ggttatgttc ccaacatcgc gcaaagcgat gcgacattac agggtttgcg
     4801 tcagctcatt tttatctggc cttgtgcact ggcaattatt gctgcattga cgatgggatt
     4861 cttttacaca ctcaatgaaa aacggtttgc attgattatt gaggaaatca accaacgcaa
     4921 aaataaagag atggcgacag aaaaaaaaac ggcttccgta acgttataaa tgtcaatagc
     4981 cgctatttcc atcctggtgg gtggcggcct ccctacgttt aaaaaatgga cttattatga
     5041 aaaagattaa tgcaataatt ttattatctt ctctgacctc tgcctcggta tttgctggcg
     5101 catacgtcga aaacagggag gcatataatc ttgcctccga tcagggcgag gttatgctac
     5161 gtgtgggtta taacttcgat atgggcgcgg gtattatgtt aaccaatacc tacacctttc
     5221 agcgagaaga tgaactaaaa catggctata atgaaattga aggttggtat cctttattca
     5281 aaccaaccga taaattaacc atccagcccg gtggcttaat taatgataag agtatcagtt
     5341 cgggtggtgc agtgtatctg gacg
//
LOCUS       DAZSNO010000215         5350 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZSNO010000215 DAZSNO010000000
VERSION     DAZSNO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.217"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(75..557)
                     /locus_tag="ACHWN2_003829"
     CDS             complement(75..557)
                     /locus_tag="ACHWN2_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933159.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="ACHWN2_003830"
     CDS             complement(625..2574)
                     /locus_tag="ACHWN2_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR8933160.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="ACHWN2_003831"
     CDS             complement(2659..2994)
                     /locus_tag="ACHWN2_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR8933161.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="ACHWN2_003832"
     CDS             complement(2994..3350)
                     /locus_tag="ACHWN2_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR8933162.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="ACHWN2_003833"
     CDS             complement(3350..4846)
                     /locus_tag="ACHWN2_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR8933163.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="ACHWN2_003834"
     CDS             complement(4843..5004)
                     /locus_tag="ACHWN2_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFR8933164.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5350)
                     /locus_tag="ACHWN2_003835"
     CDS             complement(5014..>5350)
                     /locus_tag="ACHWN2_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933165.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1276 a         1561 c         1150 g         1363 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg taattttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5341 ttcatcacgc
//
LOCUS       DAZSNO010000216         5347 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZSNO010000216 DAZSNO010000000
VERSION     DAZSNO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.218"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..171
                     /locus_tag="ACHWN2_003836"
     CDS             <1..171
                     /locus_tag="ACHWN2_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8933166.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="ACHWN2_003837"
                     /gene_synonym="chbF"
                     /pseudo
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="ACHWN2_003837"
                     /gene_synonym="chbF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="ACHWN2_003838"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="ACHWN2_003838"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR8933167.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHWN2_003839"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHWN2_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR8933168.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="ACHWN2_003840"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="ACHWN2_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR8933169.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1319 a         1187 c         1400 g         1441 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcatg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tattttt
//
LOCUS       DAZSNO010000217         5341 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZSNO010000217 DAZSNO010000000
VERSION     DAZSNO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.219"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            355..876
                     /locus_tag="ACHWN2_003841"
     CDS             355..876
                     /locus_tag="ACHWN2_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR8933170.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            932..1165
                     /locus_tag="ACHWN2_003842"
     CDS             932..1165
                     /locus_tag="ACHWN2_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFR8933171.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            1229..3187
                     /locus_tag="ACHWN2_003843"
     CDS             1229..3187
                     /locus_tag="ACHWN2_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR8933172.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3242..3676
                     /gene="psiB"
                     /locus_tag="ACHWN2_003844"
     CDS             3242..3676
                     /gene="psiB"
                     /locus_tag="ACHWN2_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR8933173.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3673..4435
                     /locus_tag="ACHWN2_003845"
                     /pseudo
     CDS             3673..4435
                     /locus_tag="ACHWN2_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            4614..4763
                     /gene="mok"
                     /locus_tag="ACHWN2_003846"
                     /pseudo
     CDS             4614..4763
                     /gene="mok"
                     /locus_tag="ACHWN2_003846"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            4705..4830
                     /locus_tag="ACHWN2_003847"
     CDS             4705..4830
                     /locus_tag="ACHWN2_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFR8933174.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(5149..5341)
                     /locus_tag="ACHWN2_003848"
                     /pseudo
     CDS             complement(5149..5341)
                     /locus_tag="ACHWN2_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
BASE COUNT         1199 a         1422 c         1652 g         1068 t
ORIGIN      
        1 aaccgcgctt cacgtcgttg cggcgggtcc ctgcccgttg ttctgtccgg tgccattcac
       61 ctgtttaagc ccctgtcgcc gggggcatag acagccacaa cctgccgcac ctgtccaggg
      121 taaatgaaca gcccacaaaa ttttttaccc aaaacccggt aaaaaatttt gcgttctgcc
      181 caggacaggt gcgtcaggcc gtggcagtga tgccccatgc ggcgaaggtt ctcaaacagg
      241 tgatggtgaa taccggaggg aacaaagagc aggtccccgc aaaacgtcaa aaccgcggct
      301 cccgaaactc aacagatgat gggggctgaa aacatcaaca gaaggagaca catcatggca
      361 gttcgtggca ttaacaaggt cattcttgtc ggtcgtctgg gaaaagatcc ggaagtccgt
      421 tacatcccca acgggggcgc agtggcaaac ctgcaggtgg ccacgtcaga aagctggcgt
      481 gacaaacaga cgggggagat gcgggagcag acagaatggc atcgcgtggt gctgttcggc
      541 aagctcgcgg aagtggcagg tgaatatctg cgcaagggcg cgcaggtcta catcgagggg
      601 caactccgaa cccgtagctg ggaagataaa ggtattaccc gttacgtcac tgaaatcctt
      661 gtgaaaacca cgggcaccat gcagatgctg gggagtgcac cacagcagaa cgctcaggcg
      721 caaccgcagc ctcagcagaa tggtcagcca cagagtgctg acacgacgaa aaaagggagc
      781 gcgaaaacaa aaggccgtgg acgtaaggcc gcgcagccgg agcctcagca gccgtctgaa
      841 cccgcgtatg acttcgatga cgacattccg ttctaacgaa agacagtgac aaccgccccg
      901 tccagtgcgg ggcatcaccg gagatatgag gatgagcgaa tatttcagaa tatttcaggg
      961 gctgcctgac ggctccttta cccgcgaaca ggctgaagcc gttgccgcac agtaccggaa
     1021 tgtctttatc gaggatgatc agggaacgca ttttcgcctg gttgtccgca ataacagtgg
     1081 cctggtctgg cgtacctgga attttgagga cggtgccggg tactggatga accatgtcat
     1141 ccggaatttc ggcattctta agtaagagag gtgccggacg ggaacgcgtc cggcagacat
     1201 acaaccatcc cggtcaaagg agaaatatat gtcagttaca gagtctaaga caaaaacgga
     1261 gcgtaaatcc agccgtaaac ctgcaaaaac gcaggaaaca gtcctgtctg ccctgctgga
     1321 gcagacggag gaagtgagcg tgtcgctggc ctcgctgatt aaatcaccgc tgaatgtgcg
     1381 cacggtgccg tattccgcag agtccgtcag cgaactggcg gagtccatta agggcgttgg
     1441 cctgttgcag aatctggttg ttcatgccct gcctggtgac cgccacggtg tcgccgcagg
     1501 tggtcgccga ctggcagcac tcaacatgct ggcagagcgt ggcatccttc cggctgactg
     1561 gcctgtacgc gtgaaggtta ttccgcagga gctggcgacg gctgcatcaa tgactgaaaa
     1621 cggtcagcgt cgggatatgc accctgccga acagattgcc ggattccgcg caatggcgca
     1681 ggaaggcaaa acacctgcac aaatcggtga tttgctgggc tattcacccc gccacgttca
     1741 gcgaatgctg aaactggctg accttgcgcc tgtcatcctc gatgcgctgg cagaagaccg
     1801 catcaccacc gaacactgtc aggcgctggc gctggagaac gacaccgcgc gtcaggtgca
     1861 ggtgtttgaa gccgcctgtc agtcgggatg gggcggtaaa ccggaagtac agaccattcg
     1921 tcgtctggtg accgaaagtg aagtggcggt ggcggggaac agtaaattcc gcttcgtggg
     1981 ggctgatgcc ttctcgccag acgaactgcg caccgatttg ttcagcgacg acgaaggtgg
     2041 ctatgtggac tgcgtggcgc tcgatgccgc cctgctggaa aaactccagg ctgtcgcaga
     2101 gttccttcgg gaagccgaag gctgggaatg gtgcgccgga cgcatggagc ctgtcggtga
     2161 gtgccgtgag gatgccggaa cataccgctg tctgccggag ccggaagcgg tgctgacgga
     2221 ggcggaagaa gaacgtctga acgaactgat gacgcgttac gacgcgctgg aaaaccagtg
     2281 tgaggaatct gacctgctgg aagcagaaat gaagctgatg cgctgcatgg cgaaggtcag
     2341 ggcgtggacg ccggagatgc gtgccggagg tggagtggtg gtgtcctggc gttatggcaa
     2401 cgtgtgtgtc cagcgtggtg tgcagttgcg cagtgaggat gacgtggctg acgacgctga
     2461 ccgtacggaa caggtgcagg agaaagcgtc agtggaggaa atcagtctgc cactgctgac
     2521 gaaaatgtct tccgaacgca cgctggcagt ccaggcagca ctgatgcagc aaccggacaa
     2581 atccctgaca ctgctggcat ggacgctctg cctgaatgtg tttggcagcg gagcgtacag
     2641 taaaccagca caaatcagcc tggaatgtaa acattattcg ctgaccagcg atgcgccatc
     2701 ggggaaggaa ggtgccgcat tcatggcgct gatggcagaa aaagcccgtc ttgctgccct
     2761 gttaccggag ggatggtcac gggacatgac gacattcctg tcactcagtc aggaggtgct
     2821 gttatccctg ctcagtttct gcaccgcatg cagccttaac ggtgtccaga cccgtgagtg
     2881 tggtcacacg tcacgcagtc cgcttgacac gctggaaagc gccatcggct ttcacatgcg
     2941 cgactggtgg cagccgacaa aagcaaactt cttcggacac ctgaaaaagc cgcagattat
     3001 cgcagccctg aatgaggcag ggctgtccgg tgccgcacgg gacgcggaga agatgaagaa
     3061 aggtgatgcg gctgaacatg cagagcacca tatgaaagac aaccgctggg tgcctggctg
     3121 gatgtgtgca ccacatccac agacagatac cactgaacgc accgataacc tggctgatgc
     3181 cgcctgatga acaaccacac cgccccgccg gagacggggc ggcagcaagg gagataccgt
     3241 gatgaaaact gaactgaccc tgaatgtatt acagaccatg agcgcacagg aatatgaaga
     3301 tatccgggct gccggaagtg atgaacgtcg tgagctgaca cacgccgtga tgcgggaact
     3361 ggatgcgccg gacaactgga cgatgaacgg cgagtacggc agcgagtttg gcggattttt
     3421 ccccgtccag gtccgtttca cgcctgccca cgaacgtttt cacctggcgt tatgttcacc
     3481 gggcgatgtc tctcaggtct gggtgctggt tctggtgaat gccggtggtg agccgtttgc
     3541 ggtggttcag gtacagcggc ggtttgcacc ggaagccgtc agccattcac tggcactggc
     3601 ggcgtcactg gacgcgcagg ggtacagcgt cagtgacatc atccatatcc tgatggcgga
     3661 aggaggtcag gcatgagcgc acgttcacag gcactggttc ccctcagcgc agagcaacag
     3721 gccgcatggc gggcggtggc ggagacagaa aaacgccgcc accagggaaa cacgctggcg
     3781 gaatatccgt atgcgggggc gtttttccgc tgtctgaacg gaagccgcag gatatcgctg
     3841 tctgacctgc gatttttcat gccgtccctg accgcagaag aactgcacgg cagccgtctt
     3901 cagtggctgt atgccatcga tgtgctgatt gaaacacagg gcgaggtctg cctccttcct
     3961 cttccaggcg atgcagcaga acgcttgttc ccgtcggtgc ggtttcgtgt cagggaacgc
     4021 agtcgccata aatccgcgct ggtcatgcag aagtacagcc gccagcaggc gagggaggca
     4081 gagcagaaag cccgtgcgta ccaggcgctg gtcgctcagg ctgaaatcga actggcattc
     4141 cattcgccgg aaacagtcgg aagctggtat gcacgctgga gtgaccgggt ggcagagcat
     4201 gacctggaaa ccctgttctg gcagtggggt gagcgttttc cgtcactggc cggaatggaa
     4261 cgctggcagt ggcaggatat gccgttctgg caggttattg cggaagccag tcttgcagca
     4321 agagaggccg gtcacgctgt ccgtgagatg gagcggtgga tggtgccgaa caaactccgg
     4381 gaggcggcgt gatgcggcaa caatcacaca gattacccgt aaacagcctg aatgagcggg
     4441 ttattttcag gaaaagtgag tgtggtcagc gtgcagggat atgggctatg atgtgcccgg
     4501 cgcttgaggc tttctgcctc atgacgtgaa ggtggtttgt taccgtgttg tgtggcagaa
     4561 agaagatagc cccgtagtaa gttaattttc attaaccacc acgaggcatc cctatgtcta
     4621 gtccacatca ggatagcctc ttaccgcgct ttgcgcaagg agaagaaggc catgaaacta
     4681 ccacgaagtt cccttgtctg gtgtgtgttg atcgtgtgtc tcacactgtt gatattcact
     4741 tatctgacac gaaaatcgct gtgcgagatt cgttacagag acggacacag ggaggtggcg
     4801 gctttcatgg cttacgaatc cggtaagtag caacctggag gcgggcgcag gcccgccttt
     4861 tcagggctga tgctggtctg actgcactga agcgccttta taaaggggct gctggttcgc
     4921 cggtggcccc tttctccttg ctggcattgt acgggcatga acaaccagac tgccaggaag
     4981 gccgtttccc tccgggaacg gggatgaacg tgcgcctgcg gcgcccgttc ttttcccccg
     5041 ccttctctgt ttatgactta ccagaaatca catcaccttc cctttgatta ttctcttttt
     5101 cctcctgctc tgactctgat tactgctctg actctgatta ctgctgccct gattattgtc
     5161 tgttttgttg ttctcctgct tatgcctttt ccgggcgtcc ccctggcggg gcgggctgtc
     5221 gtgaacggag ccagcgaagc tgtctccgcc ctggcgggct tccatccctg acgcaaaaac
     5281 ccgggtttgc agcgaccgga acggtcgcgc cacccttgtc gcgccgtggc ctgcgctccc
     5341 g
//
LOCUS       DAZSNO010000218         5339 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZSNO010000218 DAZSNO010000000
VERSION     DAZSNO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.220"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHWN2_003849"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHWN2_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR8933175.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKGRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACHWN2_003850"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACHWN2_003850"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR8933176.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHWN2_003851"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHWN2_003851"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR8933177.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACHWN2_003852"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACHWN2_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR8933178.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1291 c         1457 g         1281 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgacct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAZSNO010000219         5287 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZSNO010000219 DAZSNO010000000
VERSION     DAZSNO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.221"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..63)
                     /locus_tag="ACHWN2_003853"
     CDS             complement(<1..63)
                     /locus_tag="ACHWN2_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933179.1"
                     /translation="MVTFETVMEIKILHKQGMSSR"
     gene            complement(150..511)
                     /locus_tag="ACHWN2_003854"
                     /pseudo
     CDS             complement(150..511)
                     /locus_tag="ACHWN2_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(850..1404)
                     /locus_tag="ACHWN2_003855"
                     /pseudo
     CDS             complement(850..1404)
                     /locus_tag="ACHWN2_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1547..1669
                     /locus_tag="ACHWN2_003856"
                     /pseudo
     CDS             1547..1669
                     /locus_tag="ACHWN2_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1931..2497)
                     /gene="ipgB2"
                     /locus_tag="ACHWN2_003857"
     CDS             complement(1931..2497)
                     /gene="ipgB2"
                     /locus_tag="ACHWN2_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR8933180.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2779..3093)
                     /locus_tag="ACHWN2_003858"
     CDS             complement(2779..3093)
                     /locus_tag="ACHWN2_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933181.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3171..3281)
                     /locus_tag="ACHWN2_003859"
                     /pseudo
     CDS             complement(3171..3281)
                     /locus_tag="ACHWN2_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="ACHWN2_003860"
     CDS             complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="ACHWN2_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFR8933182.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4654..4791)
                     /locus_tag="ACHWN2_003861"
                     /pseudo
     CDS             complement(4654..4791)
                     /locus_tag="ACHWN2_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4873..5171)
                     /locus_tag="ACHWN2_003862"
                     /pseudo
     CDS             complement(4873..5171)
                     /locus_tag="ACHWN2_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1427 a         1167 c         1060 g         1633 t
ORIGIN      
        1 ccggctactc attccctgct tgtgcaggat tttaatttcc ataactgtct caaaagtgac
       61 cataagctct cctgaatcag gagagcagat taccccctgg atctgatttc aggcgttggg
      121 tgtggatcac tattgcaccg ttcgtgacag tgttgttgcg ccttgctgcc aacgaagctc
      181 cactgcccat caagttcaca gataagggca acatcagcgt gagcgactgg cgcagatgtt
      241 atccgccggg gcgggagctt tttaatgtcc gtatgacggt attgatgccg attttcagcg
      301 tacggccagt atcgcgaact ccggcaccgt tgaaagccat ttcagtgatt tggtttttaa
      361 ctcccggttt acgggcctcg taacagtaag tgagctgaaa cacccggtga cagtcacggc
      421 aacggaatcg atcatgtcct tttgggttct gcccgtgacg gtaaacctga acggactggc
      481 aacgggggca atgaaagtta actgtgacca tgaaagaacc taaaagcggg tattataacc
      541 cctaattcaa ctaattggag gcattgtcat ttttaatcta tggatacact taaaaatcct
      601 ctcaccttac gttaaaaacc ttctcatgtt tagagttttt tattatgttt atcataatga
      661 tatgacgatc tgagttttta aaatcaagaa gaaaagataa gttttttaaa tgcacagtgc
      721 ctgtggataa ctttgcgtaa atccggagga cagatcactc tgaagcaaga caacagcatc
      781 tgaaaacaat atgctcacgt ttcaaccaca gggcgtaagt gatcctaccc acgtaatatg
      841 gacacaggcc taagcgaggt tctggttttc aaattgttcc ggactgaggc cgccacacca
      901 actgtgccgt cgccagcgat tgtaatcaca ttcgatataa ttaaacactg ttgccctcat
      961 tatttcccgg ctgataaagt gttctccgtg gagacattcc actttcagcg aatgaaagaa
     1021 gctttccacg caggcattat cgtagcagca gccctttgcg ctcatactgc cacgcagatt
     1081 atgccacttc agcagcgcct gataatctgc tgaacagtac tgacttcctc ggtccgagtg
     1141 aacgataacg ttccggggtc tcttacgccg ccacagcgcc atctgcaggg catcgcaggc
     1201 cagttgcgcc gtcaggcgtg gtgacatgga ccagccaata acggcacgtg accacaggtc
     1261 aatgaccaca gccagataca gccagccttc atctgtacgt aagtacgtga tgtctcccgc
     1321 ccacttctgg ttcggaccac tggcgtaaaa atcctgctcc agcagatttt ctgacacagg
     1381 caggccatgt gcgcggtaac tgaccgtatt gctgggtaag atcaatttgc tcactggtat
     1441 ttactacgtg catcgaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt
     1501 tccgtaagca gccggagagt gaaactctct ccggctgact cgactcgcag gttccgacag
     1561 cggtgacgaa catgcagcgg tgttgttctc gctgatcggc acatgccgtc tgaacaatgt
     1621 ggagccagaa aaatggctgc gttacgttat tgaacatatc caggactgac cggcaaaccg
     1681 ggtacgcgat ctgttgcctt ggaaagttga tctgacttct cagtaaatat caatatgatt
     1741 ctgacgagcc gcttaccttt gagcagagaa aggggagagg gatcccacat ccatgagcta
     1801 ggaaaataca aaaactggct atacaacaat tcaatatatt aggacttact tgcgaggaga
     1861 aatatagcac cgctcaggca attcctgggt atttactttt cttagtttct ttactcggga
     1921 tgttctgtca tcagaaaggc gattctaaat ttgtaatata gtcactcata ttcccacgta
     1981 atttctttag atcaatatat tgcacatttt tctcaaaaaa ctgatcggaa ataagatttg
     2041 caacattgct tgttgtatca tttttaacgc ttctattcat cccttcacaa agttcgtcca
     2101 ttttcttttt gaaataactg cttcctgata caagatgatt tatggaactt tgtgcagcat
     2161 tgatgtctag agaaatgcga taatctttct caacctgttt aaatatctgt ttacggagat
     2221 tatcgccgat ttttcggctg tgttctgcac aaaacttatc aatacattgg tttaccaccc
     2281 gggatacgta gacttttttt tcatcatttt tccaatctgt aatattttca ccgatttgtt
     2341 ctgaaacaga gctcaaaata ctagtattta ttttggttga tgatattttt actatccttt
     2401 tccccattgt acagcggata atctcatcca cctttcctga aaaatatctt ttgtgagata
     2461 aactgattcc aaaattatta aaagatgttc caagcattat aatcaccttg tcatgtaaaa
     2521 tagtaacatt gtcattattt tagctgattc aaacaattct gtaaatgctt tgagttcttt
     2581 actcccttgg ctcaatgatg gaccgtctgg aatcccagag actaaactct ctgagaaaga
     2641 gagtatgaaa aaacttgggg cagtccctaa atgaagggac caacagcggt aaaataaccg
     2701 gcatcattca taatgatgcc ctgttagttg tagttatgaa gatttacgtc attaaaattg
     2761 ctgttcgtag tgtcagtcct atggtctggc atcagctgag gatcgtagcc gatacatcac
     2821 tggcttcacg acattcgtgt tgaggctatt catgaaaact ccacgctgaa aacgccgttt
     2881 tgtataaacg gtcacggtat gcccggagcc actgttgctg ataaattcga taaaaccttg
     2941 gcgtttcttg aagctatcgt taatgcggat aatgaaacaa cgattggtga gattcgctct
     3001 tttgccgacg ccctggacgc tgtccggttc aatcgcaaca aaatcaaccg gcagctgagc
     3061 aaacccaacc ttgcctctct ggcgctggag catgaagtga tttggctggg acgtagccgc
     3121 tgacagcttc tataaaagta tgatcctacc cacgtaatat gaacacaggc ttaagtgagg
     3181 ttctggtttt caaattgttc aggactgaga cctccacacc aactgtgccg tcgtcagcga
     3241 ttgtaatcgc acttgatata attaaacact gtatatttca tttgtggagt ctccagtctg
     3301 agagggcttt atgcattccg tattgggcac tatgatgcca gaaatctgca aggctccaca
     3361 ccttcctgta tttcccttcc tctatcacag tgcactttac ttaatagggt gggatgtgtt
     3421 cattacattt attgtgtttt ttatgattgt ttttgcgtcc agtgtcgttc gtcatgggaa
     3481 actggttcta tgggtagtat cgctcagtct agttaactgt ttgtttcttt ttagaaatta
     3541 agcagatcac acaattcggg gagagatcct tctaagtgca attctttgca gctatttagc
     3601 acacttaaaa caactcttgt attttgtcag aaataatatt aaacatgtct tgttcattat
     3661 tgttaagtct gtctgctatt tctgtaagtt ttatcccact atgatggcat atatttatat
     3721 atgccatcat agcctcgata ttccctttcc ctgcggccag aaataatcct ggtaacccct
     3781 cattatctct tacatccaat agtttaatcg tttcttttgg tgataaatca aaagttttaa
     3841 taaaatctcc ataagcttga atcgcagcgg aatgaccttc ttgaagagct atgaacaaag
     3901 cagattcccc agcattattt cgtgctgcaa gaacttctag tttttcctct ttgggtattt
     3961 cggctgccaa cagcgattca ccgtatttac gcgctgcatc ggcatttcca tgccaaatca
     4021 atgaaaacaa attatggcgg ttatactcct tattattctt tgtcggaatg gatatgttct
     4081 gtattttggc caattttctg aaagaaacac cactatatcc cccccctgat ttttcttcat
     4141 tgatggcgtg ggatatagcg gtatttgtca cgttaatgat attatttttc attcctttat
     4201 tttcattagc agtattgctg cctattatta agtgcatatt tttgttgttt gctggatgta
     4261 atccatagtt atttattgac atttttgttc atcctttttg ctaaaaaaaa acaataacct
     4321 atttttgtaa tgtaatagat attagtctat tgctaatcta aaaaaaccat tgccacattg
     4381 aatggctata ttcaccaata aattctttcg atttcgtata ttgacatatc gtagatatat
     4441 cttatcccta agattcaata atcgagtccg tttaccattc tgtgcaagcg ccttcactca
     4501 gaagtgaccg tcaaggcgat caccaaactt gataataaag cagctcattg ccatacgcca
     4561 gtccttaagg ggcgttgtcc atttctgtga tgctgcctag atttaactgc cagctccggc
     4621 ttatctacat ctaaatatgt gagcctaata ccgctacact ttgtccagac tatgtttttc
     4681 tccgggaagt gggctaaccg tctccataaa gtgacgaact tttttcaaca gtggccatat
     4741 tgaacggcac tgatgattac gggttattgt ctcatgaaga gccggccaca tcactgacag
     4801 cagtgacttc aggccttcag caccagacag cctgaaccag ttaatccatc gcccgacaaa
     4861 tttagtaatc tagggctggt ttttcttcag ctgttgcggt tcgaaagtgc cattacgatc
     4921 acgcagagta cggatttcca gaggttcatc tccagtagtg ataatcttcg ttaagcaacc
     4981 attgcgggaa ttgctgccag atctaagctg atttttctcg cacccaagat gatgagtcat
     5041 attagcattg agtacggttt cattttcagc atccaatcaa actgactgag atcttcagga
     5101 tttttgagac ttttggctag ttcattagct aaagcccgca acttattaga tgtcaactgt
     5161 ttttcattca taaattcacc ttcatttgat atcaaattaa atagttcaac atcagagaat
     5221 tatacaaact tatgtacagg ctcctatgat cttcgaatcc tgaagattat agtgaaaata
     5281 agttttc
//
LOCUS       DAZSNO010000220         5270 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZSNO010000220 DAZSNO010000000
VERSION     DAZSNO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.222"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHWN2_003863"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHWN2_003863"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR8933183.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHWN2_003864"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHWN2_003864"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR8933184.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3324)
                     /locus_tag="ACHWN2_003865"
                     /pseudo
     CDS             complement(2698..3324)
                     /locus_tag="ACHWN2_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3454..5214
                     /gene="ycaO"
                     /locus_tag="ACHWN2_003866"
     CDS             3454..5214
                     /gene="ycaO"
                     /locus_tag="ACHWN2_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR8933185.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1345 a         1357 c         1262 g         1306 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatcac attaccaggc
     3061 ggtataacaa cgccagagtg ataaagacga taagtaccgg caccatcggc acatcatcat
     3121 aaaacgccac atctcccgcc gctgaaccca gcgtgagaat gattaacact tcaaacagcg
     3181 acatctgtcg cacaccgcgt cttccggtca ttttgaggaa caaaaaacca gtacaaaggt
     3241 ataaagacta cgtagtgcaa cttcgccaag gaaatcaaaa ggcactttat caaatgccat
     3301 acggtggaga tcgaacgcct tcatttttat gctcttaacg tggtttatcc gctaaaagca
     3361 tagtcaacag ttcatcggca tggcggcggt gatagcgcct ttgagataaa acccgtatta
     3421 ttgcgcgctt tccgtacgac taaagtgatt ttcatgacgc aaacatttat ccccggcaaa
     3481 gatgccgctc tggaagattc catcgctcgc ttccagcaaa aactttcaga cctcggcttt
     3541 cagattgaag aggcctcctg gctgaatccc gtgcctaacg tctggtctgt acatattcgc
     3601 gacaaagagt gcgcactgtg ttttaccaac ggtaaaggcg caaccaagaa agcggcgctg
     3661 gcttctgcac tcggtgaata tttcgagcgt ctctcaacca actacttttt tgctgatttc
     3721 tggctgggcg aaaccatcgc caacggtcca tttgtgcatt atcccaacga aaaatggttc
     3781 ccactgagcg aaaatgacga tgtgccagaa gggctgctcg atgaccgtct gcgcgcattt
     3841 tacgatccgg agaatgaatt gaccggcagt atgctgattg acctacaatc cggtaacgaa
     3901 gatcgtggta tttgcggtct gccgtttacg cgccagtccg acaatcagac cgtttatatt
     3961 ccgatgaata tcattggtaa cctgtacgtt tctaacggta tgtccgctgg caatacccgc
     4021 aacgaagcac gcgttcaggg gttgtccgaa gttttcgaac gctacgtgaa aaaccgcatt
     4081 attgctgaaa gcatcagctt gccggaaatc ccggcagacg tgctggcgcg ttacccagcg
     4141 gtggttgaag cgatcgaaac actggaaaca gaaggtttcc cgatcttcgc atatgatggt
     4201 tcccttggcg gccagtatcc ggtgatttgc gtggtactgt ttaatcctgc aaacggcacc
     4261 tgctttgcct ctttcggtgc gcatcctgat tttggcgtag cactggaacg taccgtgacc
     4321 gagctgctgc aaggtcgtgg cctgaaagat ttggatgtgt ttactccgcc aaccttcgat
     4381 gatgaagaag tcgctgaaca taccaacctc gaaacgcact ttatcgattc cagcggttta
     4441 atctcctggg acctgttcaa gcaggatgcc gattatccgt ttgtggactg gaatttctcc
     4501 ggcaccacgg aagaagagtt tgctacgctg atggctatct tcaacaaaga agataaagaa
     4561 gtttatattg ccgattacga gcatctgggc gtttatgctt gccgtattat cgtgcctggc
     4621 atgtccgata tttatccggc tgaagatctg tggctcgcga ataacagtat gggcagccat
     4681 ttacgtgaaa cgattctttc gctaccaggc agcgagtggg aaaaagaaga ttacctgaac
     4741 ctcatcgagc aactggatga agaaggtttt gatgacttta cccgcgtgcg tgagctgttg
     4801 ggtctggcga ccgggtcgga taacggttgg tacaccctgc gtatcggtga attaaaagcc
     4861 atgctggcgc tggctggtgg cgatctggaa caggctctgg tctggaccga atggacgatg
     4921 gagtttaact catcagtatt tagtccggaa cgcgccaact attatcgctg cctgcaaacg
     4981 ttgttattac tggcacagga agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt
     5041 cgcatgtacg gcgcagatgc cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg
     5101 ttttacggcc tgcaaccggt agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg
     5161 ttgaaggcct acgaaaagct gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa
     5221 atagcctacg caatgtaggc ttaatgatta gtctgagtta tattacgggg
//
LOCUS       DAZSNO010000221         5149 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZSNO010000221 DAZSNO010000000
VERSION     DAZSNO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.223"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            189..446
                     /locus_tag="ACHWN2_003867"
                     /pseudo
     CDS             189..446
                     /locus_tag="ACHWN2_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHWN2_003868"
     CDS             complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHWN2_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR8933186.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHWN2_003869"
     CDS             complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHWN2_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR8933187.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHWN2_003870"
     CDS             complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHWN2_003870"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR8933188.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3071..3214)
                     /locus_tag="ACHWN2_003871"
                     /pseudo
     CDS             complement(3071..3214)
                     /locus_tag="ACHWN2_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3214..3430
                     /locus_tag="ACHWN2_003872"
                     /pseudo
     CDS             3214..3430
                     /locus_tag="ACHWN2_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3604..4419)
                     /locus_tag="ACHWN2_003873"
     CDS             complement(3604..4419)
                     /locus_tag="ACHWN2_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8933189.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4516..>5149
                     /locus_tag="ACHWN2_003874"
     CDS             4516..>5149
                     /locus_tag="ACHWN2_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR8933190.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYT"
BASE COUNT         1374 a         1175 c         1225 g         1375 t
ORIGIN      
        1 ctattttacc gcgacaattc attcagaaca tcaatagtca gggaaggaag tagcaacatt
       61 agctaactaa tcaatgcgtt gattgtaaat ccagctaaga ggtgaggttt tcagagcaga
      121 caacggtgaa acgtcatggt attgttacgt ttaggtaaca agaaatttgt ctgcacaagg
      181 attacatcat gattatggcg aaactgaagt cagcgaaagg gaagaaattt ctctttggtt
      241 tgttggcggt tttcattatt gcggcgtcgg ttgtgactcg cgcgaccatc ggcggcgtta
      301 tagaacagta caatattccg ctgtctgagt ggacgacatc aatgtatgtg atttagtcat
      361 cgatgatttt tgtttatagc ctggtcttta ctgtgttgct ggcaatcccg ttgggaattt
      421 atttccttgg cggcgaagag cagtaagtaa aaaataggcc cgataactcg ggccttgtca
      481 gttattgaag agtcgttaat cgtcttcttc gtcatccagt tcaacgggtg tctgatactg
      541 gtcaggttta atgaccagca ggtcgcagcg aagatgatca atcacctgtt ccgccgtgtt
      601 gccgaggaat gctgctgaaa taccggtgcg tccgaccgtg cccagaacca caattcccgc
      661 ctgtaaatgc tccgccaaat caggaatcac ctcttcgggc agaccttttt ctacgtgcgt
      721 catgttttca ttaatgccga atttctgccg cagggctttc attgccagca aatgttgccc
      781 acgaatggca tcgttataaa cgctcgggtc aaattccggc agttcaatcg cgatattaat
      841 tggcgttacc ggataagcgc caaccagatg aacttcggta tggttgactt gttctgccag
      901 ttcgatcgtc tctttgacca gtttttcatt gagcgcatta tgatacggct cttcactggc
      961 gagattcacc gccaccagcg ccttgcctcc ttccggccac ggctggtctt tcaccatcca
     1021 caccgggctt gggcatttgc gtaacagatg ccagtccgtt ggcgtaaaaa tcaccgcttc
     1081 cagacggtca tgttggtgcg ccatttttag caccaaatcg tgtccgccgc tgatcacttc
     1141 ctgaatgatg gcttcgaaag gacggttatg ccagaccact ttaatttcaa tgggaacgcc
     1201 agcattgaga taatattttg cctgctcgtg gatccaggct gtacgctggc tgatgacgcc
     1261 ctgacgcata gcggtacgtt cgtccgggga gagcagggtg gtcatttcgt atgagaagtc
     1321 atagatcggc aaaaaggctt taattttgcc accaatccgt tgatgtaaat aaacagctcg
     1381 ccgcaatgct ggttggtcgt cctggttagg atcgataaca acgagcatgt tctgatacat
     1441 agccatacag ggtctcctta caacaactgt caacgcagtt tgtaattaaa agattaaccc
     1501 atatctggtg aatgaaacag tgatgaacct tctgccagat caataaatca gaaaaattta
     1561 atgatatgac agaaggatag tgagttatgc ggaaaaatca ggcaacgtta cgcgtatggc
     1621 cagcaagctg ggccagcgca tcgttatttt cgatggtgat gtatttacct ttgactgcca
     1681 gcataccgct tttctggaag cgacccagca gacggctgat ggtttctacc gtcaggccca
     1741 gatagttacc aatatcgcca cgagtcatcg tcaggcggaa ttcacgaggg gagaagccgc
     1801 gttgggcaaa acgacgggac aggttgtaga tgaatgcagc cagacgttcc tcggcatttt
     1861 tcttcgacaa cagcaagatc atgtcctgat cgcctttgat ttcaccgctc atcagacgca
     1921 tcatctgctg acgcagattc ggcattttac cggacaaatc gtccagcgtt tcgaacggaa
     1981 tttcacatac catcgaggtt tccagcgcct gagcgaagct cgggtgatgg ccgctgccga
     2041 tggcgtcaaa tcccactagg tcgcctgcta aatggaaacc agtgatttgc tcgtcgcctt
     2101 gctcagtgat tgtataactt ttaatcgtac cggagcggat ggcataaagc gatttaagtt
     2161 catcaccagc cttaaacagc gtctggcctt tctgaatagg cttcttccgc tcaatgatat
     2221 tatcaagctg atcaagctca tgttcgttga gtgtgaacgg gatgcaaagc tggctgatgc
     2281 tgcaatcctg gcaatggata gcacaaccgc cagactgaat gcgccgtata attcgctttt
     2341 ccgggatcat aggtctgctc aagccgtaat tgatatttgt caattttaac atctttttag
     2401 ggagcaagta agtctaagca aaccttaaca gcagagaatt ccgatattag atgtaaatat
     2461 atgtctatct atttgaaact ccttaagttg tcaagggtaa ctttacataa aagtgtgaac
     2521 aagctggcac aaattgttta atgtttacag caaaagataa ccttcgtggc gcaataacca
     2581 ctcttttcgc tgaactccgc ctgcatatcc ggtcatggtg ccgtttcggc caataacccg
     2641 atggcaaggt acgacgatgc tgatgggatt cgatccgttt gccgcaccaa cggcacgcgc
     2701 cgcgccaggg cggcccaatt gttcagccag ttggccgtaa tgcattacct gcccgcaggg
     2761 gatagtgcgt agtgttttcc agacttcgcg ctgaaatggc gtcccccccg tggcagtggg
     2821 aagcgtatca ataatgctaa gattaccggc aaaatattca cgaagcttgt cgcttaaacc
     2881 acctgggttg gtggcagaaa tgcgctcata gccttctttg cgataatgga tgtccagcag
     2941 ctgcaccatg cgttcgctgt actcttccca ttcaaccgcc cgcaggcgaa attgctcatc
     3001 gcaaatcacc cacagtggac ccagtggcgt ggcaattttt tcttcaagta atctcagcat
     3061 ccgttctctc ttaagacaaa cgtgggtaaa taccctgacc tatcggcaga ccgacaagat
     3121 accacgccag caacatcagc agccatacca ccaaaaagat aagtggatag ggcaagacta
     3181 acgaatagta agtacccagt ttcgcgtctg gtttgtcgca gtctgatagc ataaagcgat
     3241 tttttaattt ttctgcctga agaaatgagg gcaagccatc acatggatcg agggtgggat
     3301 aattccagtc agtgaaattt taccgtaggc tatctgttat ttgattctgt ggcgtgatag
     3361 ccgccagaca ccactgaact cgtcactcat tccccaatcc cggcgagaat gtggttattt
     3421 acagagctaa aaaatgaata aaaaaataga gcctgtcgac atccgccaga ctctacagta
     3481 cacacagcag tgcatcctcg tcttaaatcc ggcctggggt cctggtgacc ggtgacagtg
     3541 ttataacggt tttacccatc cgttgccaat tttcttcagt gacagatttc acgaaaatat
     3601 aagttaaatc atttgtcttg tgattgcata attatgcatc gttaatcgac gagtaaattt
     3661 accgggcgag tagcccgttc gacgcttaaa catattgccg aacgattcag ctgaggcata
     3721 gcccagacga tgggcaattt tggtcaacga atatccatca tcaagaagcg tcactgcaga
     3781 aataacgtgc atttgctgaa cccagtggcg aaatgttaat cccgtctctt taacaaataa
     3841 gcgcatgagt gtacggctac tgacgttaat tttatccgct agattatcca gtgttggcag
     3901 atacccttgg ttgtttagca aattctcaca aagctgctgt agccgctcat catttggcca
     3961 agggagttca agtgacattt tcacccccat ttttatttcc tgaagtatca gtgtctcgag
     4021 ggctgaaaaa cgtaactgtt gtttatctgt ggtactttgc gacggtggaa gttggttaat
     4081 tgcaattagt aattccctta acaggggtga aatacccgtc atgaagcaaa ctgttcccag
     4141 cgtttcactt tccgcttctg aaaccagtac ggtttttaaa agtacgttgc ctgtcgcaac
     4201 aaacttatgc ggcatttgtg ggggcagcca aagcgcgctg ctggcatgga ctagccagac
     4261 gctttctcca gcccatacac gaacgactcc ttcagccggg taaagcaact gggcatagag
     4321 atgtttatgc cagggaactg tacctccatt cagatgttcc gaatccagtc cgataatgtg
     4381 atgccctggt gatgctttgg gttgcagtgt aattgtcatt tacagtaggt attttgtcag
     4441 aaatcatcaa ataagccatg ttaaatttta aatatgacgt atgcaagttt ccggatatat
     4501 acggtgtaaa gagtgatgat aatttctaaa aaacagctta ttggggtggt agcgatcggt
     4561 attcttttga ctggcgttgt cttttttatc tggagggtaa gcaaaggaag atttatacag
     4621 acgacggatg atgcttacat tggtggtaat atcaccacgg tggcaagtaa agtttctggt
     4681 tatatttctg ctatagaggt gcgcgataat cagagcgtaa aaaaaggcga tattatcctg
     4741 cgtctcgatg atcgggatta tcgtgcaaat gtcgccaggc ttgaggcaaa aataaaaagt
     4801 agcaaagcca atctcgaagg cattcaagcc actattacga tgcagcagtc gataattcaa
     4861 agtgccagtg aaacatggca ggcagtaaaa catgaggagc aaaagcgcct gcgagatact
     4921 gaacgttatg aaaaactcgc acaatcagcg gcaatatcgc aacaaattat tgataacgcc
     4981 aggtttgact accagcaggt cgcagcaaag gagcgcaaag ccgcgaacga tttcctggtg
     5041 gagaagcaac ggctggcagt tttatctgct caggaagaaa acgtacgtgc gagtatcgaa
     5101 gaagttcagg ccgctttgac tcaggcacta ctggatctgg aatataccc
//
LOCUS       DAZSNO010000222         5087 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZSNO010000222 DAZSNO010000000
VERSION     DAZSNO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.224"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..244
                     /locus_tag="ACHWN2_003875"
     CDS             <1..244
                     /locus_tag="ACHWN2_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933191.1"
                     /translation="EMLSFLTSEEREALTNMLARKGVETVLRLLDKETQELLQLEGRK
                     RLAALMLVDMPDEQVREILEEIEASILSESVNAKAG"
     gene            493..1437
                     /gene="tauA"
                     /locus_tag="ACHWN2_003876"
                     /pseudo
     CDS             493..1437
                     /gene="tauA"
                     /locus_tag="ACHWN2_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="ACHWN2_003877"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="ACHWN2_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR8933192.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="ACHWN2_003878"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="ACHWN2_003878"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR8933193.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="ACHWN2_003879"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="ACHWN2_003879"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR8933194.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHWN2_003880"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHWN2_003880"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR8933195.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1155 a         1297 c         1462 g         1173 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatct
//
LOCUS       DAZSNO010000223         5042 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZSNO010000223 DAZSNO010000000
VERSION     DAZSNO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.225"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..325
                     /locus_tag="ACHWN2_003881"
     CDS             <1..325
                     /locus_tag="ACHWN2_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR8933196.1"
                     /translation="AWRAEQEAKLFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQT
                     HMAWSDADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQRELKEELNSLKDLNLVR
                     NFIVE"
     gene            complement(526..1383)
                     /gene="sitD"
                     /locus_tag="ACHWN2_003882"
     CDS             complement(526..1383)
                     /gene="sitD"
                     /locus_tag="ACHWN2_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR8933197.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1380..2237)
                     /gene="sitC"
                     /locus_tag="ACHWN2_003883"
     CDS             complement(1380..2237)
                     /gene="sitC"
                     /locus_tag="ACHWN2_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR8933198.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2234..3061)
                     /gene="sitB"
                     /locus_tag="ACHWN2_003884"
     CDS             complement(2234..3061)
                     /gene="sitB"
                     /locus_tag="ACHWN2_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR8933199.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3061..3975)
                     /gene="sitA"
                     /locus_tag="ACHWN2_003885"
     CDS             complement(3061..3975)
                     /gene="sitA"
                     /locus_tag="ACHWN2_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR8933200.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4407..>5042)
                     /locus_tag="ACHWN2_003886"
     CDS             complement(4407..>5042)
                     /locus_tag="ACHWN2_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR8933201.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1332 a         1232 c         1212 g         1266 t
ORIGIN      
        1 agcatggcgc gcagaacagg aggcgaagct gttcacgttt gaatggaacg gtcgtatctg
       61 gaatgctggt cccgactcac tgggccgcct gtccccggta gtcatgctgg caaaatctgt
      121 cacagcacaa acacatatgg cgtggagcga tgccgataat cagcaggtga aactgtcgat
      181 gccggaactg gaagaactgg cggcagcaat ggtgcaggcg caggtcgatc gcaacgatga
      241 gatttatcgc cgtcagcggg agctgaagga agagctgaat agtctgaagg atttgaattt
      301 ggttaggaat tttatcgtgg aataacagaa gctgcggcac gtcgtatgca ggaacgtgcc
      361 gcggttggct ggtaaacttt cgatagtgcg agtattgaat gatttctagg cgttatcgat
      421 tttacgtatt ttttgcatga tagaattcgt acctcctccc aacgaccttc catggctttc
      481 cggacttctg taggctggcg gacgatgctg tcctcggatg ttgtgctaat tgggtgataa
      541 cagatcctgt gtatgggcgt ttccctttgt gcgtgcgttg atgctggtaa cggtaaacgt
      601 catgatgaac acgaccgcga ataagacgac aatcgtcgga gctggtgcgc tgtcgataaa
      661 aaaggacagc cagacaccag tcattgacac tattaccgat atgccagtcg ccagtaggag
      721 cgcaatatga aagcgttgtg tcaggagtac tgcaatggcg ccaggcgcaa tgagcagaga
      781 aatagagaga atgataccta ctgctttcag cgtcgccaca atggtcaggg aaaccatgca
      841 cagcagccca taatgcagcc atctcgtatg caaaccgctt acctgcgcct gttgataatc
      901 aaagctgaac agcaaaaaat cccgccattt tacgctaata acaagtgtaa ccagcccggc
      961 aataatcacc gtctggatta tgtcgccgat ggttatcccg agcatgtcgc cgaaaagaat
     1021 atggtcaaga tgtacgtctg gtttaattgc aatatacaag atcagacctg cggcaaacat
     1081 gccagaaaaa acaatcccca taactgtatc ttgcttgatt cggctgttgt ctttcaggta
     1141 tcccgtcgcc accgcgcaga atactccggc gacgaatgcc cctgtagcca gaggtaaccc
     1201 cagaatccag gtaagcacga tgccaggaaa aaccgcatga ctcatcgcgt cacccatcag
     1261 tgcccaacct ttcaggacca gaaagaccga cagcagcgca cagggtatgg aaactaccag
     1321 tgaaatcagc agtgcgtgac tcataaaggt gaactgcagt ggctccagta ataatgccat
     1381 catgagcgat cctccagcgc tttatgcgca cgccgacgat ttgccaaaag tccatgggtg
     1441 ggcgcaaata caaatgctaa aagaaacagc agtgtctgcg ccaccacgat gattccacct
     1501 gtcgcgccgt ccagaaagta actgacccac gcgccgagga aactggttac actgccaata
     1561 gttacagcaa ttatgagcag gcggggaaaa cgatcggtta acaaccatgc tgttgcaccg
     1621 ggagtcacca ccagacagat caccagaaag gcaccaaccg tttgcagcgc cgcaacggtc
     1681 gaaaccgaca gcagggtgaa gaagaggatt tttaatctgc ccggatgtaa tccaatagca
     1741 cgggcgtgat tctcgtcgaa aaaggtcacc ataagatctt tccatttgaa aaaaagcacg
     1801 atgatcgaga gaatgccaat gatagtgagt tgcaggatat ccgccggatc gattgccaga
     1861 atattgccca ggacgatggt ctgaatgttc acggatgtcg ggttcaatga caccataaac
     1921 agccccaggc caaaaaagga cgagaagatc aggccgatga tggcatcttc ttttaagcgg
     1981 gtgcgttggt taagaaacag catgctgccc gctgccagtc cgccggaaaa gaaggctccc
     2041 agagaaaacg gaagccccag catatatgcc cccgcaacac ccgggacaat agagtgcgac
     2101 agcgcatcgc caatcagcga ccagcctttt agcatcagat agcatgacag gaaagcgcag
     2161 agaccgccga ccattgccga gacccacatc gcattgagca tatattcata gctgaagggt
     2221 tccagtagca cgttcatctt gcttccctct gcaccgctga cggtcgatgt gccacaaaag
     2281 ggcgttcatc gtcagtaata atgctttctt ccgagccgtt aagtgtgaca tggcgcagta
     2341 cgccgctaaa agccagctcc aggttctcgg ctgtaaaagt ggtgtctgtc ggcccgcttg
     2401 ccaggacggt gcctttgacc atgaccgtat aatcgcaaaa tgtcgtgact gagccgagat
     2461 tgtgcgttga gacaagcatc gttttgcctt ccgcgcgtaa ctccctcaac aggctgatga
     2521 ttttcgcttc ggttttcaca tcaacgccgg taaaaggttc atccagcagg atcacatcac
     2581 cctgctgtgc aattgctctc gccagaaaga cgcgtttctt ttgtccaccg gaaagctcgc
     2641 cgatttgacg atggcgaaaa tccaccatat cgacacgttc cagcgcatcg gtgacaatct
     2701 gacgatccct ctttttggct atacgcaaca tacccatatg cccatagcgg cccatcatca
     2761 ccacatcttc caccagtaca ggaaatgacc agtcaacctc ttctgactga gggacgtagg
     2821 caaccaggtt tttctgtagc gcctgtcgcg tgggaatacc cagaacagat atctttccgc
     2881 tggtcagacg cacaaatccc ataatcgctt taaacaacgt cgattttccg gaaccgttta
     2941 ccccaaccag agcggcaata gatccacttg gtactgtgaa ggatgcatcg cgcagtgctg
     3001 tgtgcccgtt acgccaggtg acagtaacat cattaacgac aatgcctgca gattgcatca
     3061 ttatttctcc cttttcccgg ctttaattcc ctgtaccagc gtactggtag taaccttaag
     3121 aaggtcgata tacgtaggta ccgggccgtt ttctgtgctc agggaatcga catagagcac
     3181 accgccgtag tgtgcgccgg tttcacgcgc aacctgacgc gctggtttat cggaaatcgt
     3241 actctcgcta aagactgccg ggatatgatt tttcttaact atatcaacaa ccttacgtac
     3301 ctgctgcggt gttccttgtt gatcggcatt aatcggccac agataaagct cttttagccc
     3361 caaatcgcgt gcgagataag aaaaagcccc ttcactggtg accatccatc gctgattctc
     3421 agggagttcc gtaatctgct tacgcagggg ggcaagggtt tgggtaatct tggctttata
     3481 agtatcggca ttacgttggt aggtttgtgc atttgccgga tcgtatttta tcaacgcatc
     3541 acgaatatta tcgacgtaaa tcagagcatt atctggcgac atccatgcat ggggattagg
     3601 tttgccctca tagggtcctt cggtgatccc tactggcgtc acacccgaag agacaattac
     3661 ttctggaacc ccattgagat gctggtaaaa gcgttggaac cacaattcca gattcatacc
     3721 attggcgaga atcagttgtg ccccctgcgc acgtttaata tcgccagggg taggctgata
     3781 ctcatgaatt tctgcaccag gcttggttat ggatgagact tctgcagcat ctccagccac
     3841 gtttttggcc atatccgcga tgatggtaaa tgttgtaatg accttgaatt tttcagttgc
     3901 acttgcctga aatgcgatcg agcaggtgag ggcgaggcac cccaagagca tggttacttt
     3961 ttttattgag tgcataattt agtacctata gtgagcaaaa ttagaacaaa catagcaacg
     4021 gctatgtttt atagcataac ctatacaaaa tgaaaataat tatcattaac agctttgagg
     4081 cctaaaacag ggattaggtt gtttcctgct ggcgacatga tattgaggaa aaaagtagag
     4141 cagctattag gctttgaaat gactttatga ggaaaattgt tgatggcaaa aatggggcaa
     4201 aaaagagtgg gttatcgtag cttattgttg tcgctgataa tgtttaacat attgaaaaat
     4261 aaataaaata cttatgagtc agatagttgt gatttttgcc cttacttgtt caggttgtat
     4321 tgttctttct ttgtaatttg ttgattttct tgcattattt cagttctctg gtactaaatg
     4381 gggcaaattg ggggcaaact ttgcaactac gataaccgcg cattcaacat ggctatctgt
     4441 tcgtcgttca tgtcatcaat ccacataccg taaatttcat acaccatttg cgcagtttca
     4501 tgccccatct ggctggctat aaatgccggg ttcgctcctg ccgtcaacag ccagcaggca
     4561 aaagtatgtc gcgtatggta cggattacgg cggcgaatac cagcacgttt tactgctgca
     4621 ttccatcttg tacccaaact gcttaccgag taataaggtt tctgtttttc gttacacacc
     4681 ctgggcatga aaacaaaatg cagtttttgt ttttcagttc tgccgtactc ccgatgataa
     4741 aaagtgattt cgcttttgcg atgatgcccg gtcagtttgt attgctcctt cagtgcttca
     4801 agagccggct gcagtaatgc taccgtccgg atcccggcat ttgtttttgg gggaccgaac
     4861 atatcaagta tcgtcaggtt tcttctgaca ttcacaattc ccttctcgaa atccacatcc
     4921 tcccacgcca gagctgccag ttccccgtga cgaagcccgg agtaaacggc aaatttccac
     4981 aagttttggc tctgtccttt ttcactttcc attaatgcat tgaattctgt tttagataac
     5041 gg
//
LOCUS       DAZSNO010000224         5024 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZSNO010000224 DAZSNO010000000
VERSION     DAZSNO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.226"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="ACHWN2_003887"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="ACHWN2_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFR8933202.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="ACHWN2_003888"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="ACHWN2_003888"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFR8933203.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="ACHWN2_003889"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="ACHWN2_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFR8933204.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="ACHWN2_003890"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="ACHWN2_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR8933205.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5024
                     /gene="yidZ"
                     /locus_tag="ACHWN2_003891"
     CDS             4378..>5024
                     /gene="yidZ"
                     /locus_tag="ACHWN2_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR8933206.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1256 g         1416 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctg
//
LOCUS       DAZSNO010000225         4978 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZSNO010000225 DAZSNO010000000
VERSION     DAZSNO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.227"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(117..435)
                     /locus_tag="ACHWN2_003892"
                     /pseudo
     CDS             complement(117..435)
                     /locus_tag="ACHWN2_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="ACHWN2_003893"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="ACHWN2_003893"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR8933207.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..>4978
                     /gene="virA"
                     /locus_tag="ACHWN2_003894"
     CDS             4416..>4978
                     /gene="virA"
                     /locus_tag="ACHWN2_003894"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR8933208.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIY"
BASE COUNT         1502 a         1140 c          836 g         1500 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacag
//
LOCUS       DAZSNO010000226         4973 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZSNO010000226 DAZSNO010000000
VERSION     DAZSNO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.228"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHWN2_003895"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHWN2_003895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFR8933209.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACHWN2_003896"
     CDS             1020..1703
                     /locus_tag="ACHWN2_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFR8933210.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACHWN2_003897"
     CDS             1700..2161
                     /locus_tag="ACHWN2_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFR8933211.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACHWN2_003898"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACHWN2_003898"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR8933212.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACHWN2_003899"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACHWN2_003899"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFR8933213.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHWN2_003900"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHWN2_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFR8933214.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHWN2_003901"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHWN2_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFR8933215.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..>4973)
                     /locus_tag="ACHWN2_003902"
     CDS             complement(4903..>4973)
                     /locus_tag="ACHWN2_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933216.1"
                     /translation="NYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1176 a         1305 c         1239 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAZSNO010000227         4870 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZSNO010000227 DAZSNO010000000
VERSION     DAZSNO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.229"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..527
                     /gene="ydcF"
                     /locus_tag="ACHWN2_003903"
     CDS             <1..527
                     /gene="ydcF"
                     /locus_tag="ACHWN2_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR8933217.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="ACHWN2_003904"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="ACHWN2_003904"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR8933218.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHWN2_003905"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHWN2_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="ACHWN2_003906"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="ACHWN2_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR8933219.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="ACHWN2_003907"
     CDS             4169..4342
                     /locus_tag="ACHWN2_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933220.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4368..4436
                     /gene="yncP"
                     /locus_tag="ACHWN2_003908"
     CDS             4368..4436
                     /gene="yncP"
                     /locus_tag="ACHWN2_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR8933221.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHWN2_003909"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHWN2_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR8933222.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="ACHWN2_003910"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="ACHWN2_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1120 c         1208 g         1295 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DAZSNO010000228         4755 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZSNO010000228 DAZSNO010000000
VERSION     DAZSNO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.230"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(192..524)
                     /gene="pemK-mt"
                     /locus_tag="ACHWN2_003911"
     CDS             complement(192..524)
                     /gene="pemK-mt"
                     /locus_tag="ACHWN2_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFR8933223.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(526..783)
                     /gene="pemI"
                     /locus_tag="ACHWN2_003912"
     CDS             complement(526..783)
                     /gene="pemI"
                     /locus_tag="ACHWN2_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFR8933224.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(876..1529)
                     /locus_tag="ACHWN2_003913"
     CDS             complement(876..1529)
                     /locus_tag="ACHWN2_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFR8933225.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(2468..3325)
                     /gene="repA"
                     /locus_tag="ACHWN2_003914"
     CDS             complement(2468..3325)
                     /gene="repA"
                     /locus_tag="ACHWN2_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR8933226.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(3318..3392)
                     /gene="tap"
                     /locus_tag="ACHWN2_003915"
     CDS             complement(3318..3392)
                     /gene="tap"
                     /locus_tag="ACHWN2_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR8933227.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(3637..3885)
                     /locus_tag="ACHWN2_003916"
     CDS             complement(3637..3885)
                     /locus_tag="ACHWN2_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR8933228.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            complement(4170..4319)
                     /locus_tag="ACHWN2_003917"
     CDS             complement(4170..4319)
                     /locus_tag="ACHWN2_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR8933229.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            complement(4566..>4755)
                     /locus_tag="ACHWN2_003918"
     CDS             complement(4566..>4755)
                     /locus_tag="ACHWN2_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933230.1"
                     /translation="MVRKFGKQLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHT
                     KNELTGGKRRYRIKNGCK"
BASE COUNT         1187 a         1260 c         1139 g         1169 t
ORIGIN      
        1 aacttcaaaa ctgcgcaaaa cacgcaaata cgcacaagaa agtagggctg cgcttggaat
       61 tcggaaaata aaccacatca aaactacttt ttagccaaat aacgtttggg aatcaccaga
      121 atggtgggac aacagcggtt ttagtgccct aaatcgtacg ttttcatcca gttgcccctc
      181 aaaccccatg ttcaagtcag aatagtggac aggcggccaa gaacttcgtt catgatagtc
      241 tccggaaccc gttcgagtcg ttttccgccc cgtgctttca tatcaattgt ccggggttga
      301 tcgcaacgta caacacctgt ggtacgtatg ccaacaccat ccaacgacac cgcaaagccg
      361 gcagtgcggg caaaattgcc tccgctggtt acgggcacaa caacaggcag gcgggtcacg
      421 cgattaaagg ccgccggtgt gacaatcagc accggccgcg ttccctgctg ctcatgacct
      481 gcggtaggat caagcgagac aagccagatt tcccctcttt ccatgtcaga tttcctcctg
      541 accagtcgcc ggtgcatcca gccattctcg ttcttcagct gatatttcag cattcggatc
      601 acactgtgcc agtagctcag ccagtgaata ttgcgggcgt ctgtacggct caacaatcag
      661 ccggccatta tcaatgacca tgccaacttc attatctgtg cccagagaca gcgcattcag
      721 cagtgccggt gggacggtca gcataactga gccgccaacc ctcttcagtc gggtggtatg
      781 cattcttcac ctccataaaa gttatattta aatataacat ccactaaaaa aacacaccag
      841 gctttaacgc acaatgttta ataaaaatat aactttcaac caaacagtaa acccagcgtg
      901 gttgcttcca tatgcagcaa taccggcagc agcaggccac cacttctgat cctggccact
      961 gatgtaatca accccaccag gaacagttct gccagtgtca gcaggttctg atactggctg
     1021 tgcgcggcga cgaacaacaa cgacgttatc agcgccccca gccacatcgt ccagcagtac
     1081 cgtgaacgga agacgttcag cataatcccc cggaacagcg tttcctcatt caacggggca
     1141 aggataaaga tggtcagcaa cgtcaggatc acgtcaggta tggacttatc ggcaaaaagt
     1201 ttcgtcataa atggctcagc aggcagagcc agcgccttac cgagcagaaa tacaccgaca
     1261 tacaccacgg ccatcgcacc gaccagccac ggtacgccaa cgttgcgcag ctgaccaacg
     1321 accggtagcg gagctatcca acggcggtat accaggaaaa cacacagcag gtacatcaga
     1381 acagtaccat gactgaagaa caaatagttt tttcctgatc cataaagcag aacggcctgc
     1441 tccatgacaa atctggctcc ccaactaatg ccccatgcag ccagcataac cagcataaac
     1501 tgcagatatt gattacgtgt ttgaatcatt gcatcgcctg taaattttta acttgtccta
     1561 tttttgtcat taccacgtat atacacatgt ataacaattc agatatcgtt accaggatat
     1621 gccgcatcag cggcatggaa ggcggcactc tgttgtttca tatgatacag gagtaaaacc
     1681 gccgaagccc ggcgtaagcc ggtactgatt gatagatttc accttaccca tccccagccc
     1741 tgccagacca tacccgcttt cagccatgag agagcttctg tgcgcggtcg gagtggtccc
     1801 gacgagggtt tacccgaagt cggggcgtat ctccgcgtta gcgggccgtg agggccgctt
     1861 acgagcgtgt actgagaact tccagcgaga agactgacag cgatgaagat gtagttacaa
     1921 cattcataat taaaagcgac tctgttccgg ccctttgggc cggggcgggg ccgcttttca
     1981 gttatgaggg aggggctttg tggtttcggt tctgcgctgg accggggttt ttctggaggt
     2041 tgtttttgtg tgttgtaact aaagtggctc cggtcggggc ccgccgcttg cggtgggagg
     2101 tgcatatctg tctgtccaca ggacaggcag tgaataggtt ttctttttaa atgaatgtaa
     2161 ttaagtagtt taaaggagat ataaacaggt gtttaaaaga tacattgcac cctgtaagac
     2221 tggcggctgg cgctttatga catgaacggt tgtaacctta tggggaagtc ccttgcagtt
     2281 aaatgtggat aagcaaaatt ccccgtcgct gaggcgtatt ttgtattaaa aacaggggga
     2341 atcggatgct ccagaaggtg gatgatgaga ttgttttttg catgcgacgc tgtttttttg
     2401 tgcaccggcg ggcttcaggc gtgcgaatgc ctccggcgcg ggccggatta ttcagaggag
     2461 gtcactttca gggggaagct gtggccagcc ggctgtaatt acggttacgt gacagaatca
     2521 tgcgctcctt cacacgacgc tcaacttcgc gttttaccgc ctcacgattg gcagtgaagc
     2581 gcccttccgc gatttcgcgc gtcagctgcc gtttcaccag ggtgacaata tcctgacgtt
     2641 ccctgtccgc atcacgacgc gcacgggcac gctttattcc ccgggactta agctctgtct
     2701 gataactgcg aaaacgctca cgaacaaaac gccaggcttt cgctatcagt tcatccatgc
     2761 ccagggtatc cagcccctgc tttttgcgtt gtttgttttc ccataccaca cggctgcggc
     2821 gcgcggcggc cgctgcctcc tctgatacat cgagggcagc aaacagtgca gatgtgaacg
     2881 tgatatcggt cggaatgtag cacccgataa gcgggtcata ttccgtctgg taggtaatca
     2941 gtcccagctc tgacaggaac gtcagggccc gggtggcacg ggtgatggag agttttccgg
     3001 cagcagactc cgtcgccagt ccgcactcaa tggccagcgt ggtgatggag cactggacgc
     3061 ggttggccag cgggtcatag tggaaacaca gcccctgcag gagcgcatca atagcccgtc
     3121 gacgcagcac cggtggcatg cgtcgacgca gaccacgcga acgggcatgc gccacatgaa
     3181 tggcgaaatc aaaacgggaa gtgaagccca ccgccttttc catcagtttt tcgcagaact
     3241 tcagcgttcc ggcaccttta cggggtgtaa acaccggatt cgggttcttt acctggcggt
     3301 aatacgtttg ttgaagatca gtcacaccat cctgcactta caatgcgcag aaggagtgag
     3361 cacagaaaga agtcttgaac ttttccgggc atataactat actccccgca tagctgaatt
     3421 gttggctata cggtttttag atggccccgg taatcttctc agtcgccaaa cttgtcgaag
     3481 attatcgggg ttttcacttt tctggctcct gtaaatccac atcagaacca gttccctgcc
     3541 accttacggc gtggccagcc acaaaattcc ttaaacgatc agcaatctat cacgcacacc
     3601 agaaacaggc aagaatgtga tgtcgctaca ttttagttag ctaaaagcac tcttctgctt
     3661 tatcagttcg gcaatcatct cagcctgggt aacgccctca gcttcacaca tcacctgcaa
     3721 ttgcgctttt aacgcagcag gaatgaacac tctgagttct ttatgtgtag ctcgttttct
     3781 tgccagggat gcctgctgac gttctgcaag cgtcagagga ttaccttttc tgtatgctcg
     3841 ctttgtacct gatgaggaag tcactgcatt ttctgtctgc gacatctcgc ctcctcaata
     3901 ctcaaacagg gatcgtttcg cagaggatac tacagttttt tgaaatcagc gacttgagaa
     3961 ttgtgacgaa gatctgtgtt ggcgcacaaa tcaacgggga ttactgttgt ttaatgtgat
     4021 ttttgttgcg aaatggctga aaaaacagga aatgccttaa cccgggagcc ggccccgggc
     4081 gggaccggct gtttctggca tgtcgggtgt ctgcccctga aatacctcac acttccggaa
     4141 agcggggact tccgtccccg cccggcagtt tacttccgtg cttcgtaggc cagagttacc
     4201 tgcaccactg tatttcccct gtgaatattc agctcacata accgttccct gaatatcagt
     4261 gaaaaacaca acaccgtggc gcacacggcg agcaacccga taagggcata cttcgtcata
     4321 aactttgacc tctctgcaag gaagaggcta caatcagtag tgcaatgtac ttcatgcaga
     4381 cctcacgagt taatggatta acaagtgggg tctttgcatt tctggtacct gcgtcttgcc
     4441 tgaaccagaa aggctcagac acccgccgcg ataataccac attccctgtt gctgactatt
     4501 acctgcattg cccgcttttt actacagaag cacgaaggcg tcactccagc caataagtat
     4561 gctttttatt tacatccatt tttaatccgg taccgacgtt ttcctccagt taactcgttt
     4621 ttcgtatgac tcatggcaat aaaattttct ttaccatatg acaacatccc aataatatca
     4681 aagttgtctt ttaatttgat ggcatccttt tcacttacag cgacaataag ttgtttacca
     4741 aattttctga ccatc
//
LOCUS       DAZSNO010000229         4745 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZSNO010000229 DAZSNO010000000
VERSION     DAZSNO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.231"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            240..2672
                     /gene="hsdR"
                     /locus_tag="ACHWN2_003919"
     CDS             240..2672
                     /gene="hsdR"
                     /locus_tag="ACHWN2_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFR8933231.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="ACHWN2_003920"
     CDS             2739..4208
                     /locus_tag="ACHWN2_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR8933232.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..>4745
                     /locus_tag="ACHWN2_003921"
     CDS             4208..>4745
                     /locus_tag="ACHWN2_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR8933233.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDF"
BASE COUNT         1373 a         1154 c         1144 g         1074 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaagctggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttg
//
LOCUS       DAZSNO010000230         4636 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZSNO010000230 DAZSNO010000000
VERSION     DAZSNO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.232"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..311
                     /locus_tag="ACHWN2_003922"
     CDS             <1..311
                     /locus_tag="ACHWN2_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFR8933234.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="ACHWN2_003923"
     CDS             468..1217
                     /locus_tag="ACHWN2_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR8933235.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="ACHWN2_003924"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="ACHWN2_003924"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR8933236.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHWN2_003925"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHWN2_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR8933237.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="ACHWN2_003926"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="ACHWN2_003926"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR8933238.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="ACHWN2_003927"
     CDS             4084..4557
                     /locus_tag="ACHWN2_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR8933239.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1239 a         1162 c         1137 g         1098 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaat
//
LOCUS       DAZSNO010000231         4630 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZSNO010000231 DAZSNO010000000
VERSION     DAZSNO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.233"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..369
                     /gene="bglB"
                     /locus_tag="ACHWN2_003928"
     CDS             <1..369
                     /gene="bglB"
                     /locus_tag="ACHWN2_003928"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR8933240.1"
                     /translation="RVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLN
                     DHLVQVNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTR
                     KKSFGWYAEVIKTRGLSLKK"
     gene            455..2071
                     /gene="bglH"
                     /locus_tag="ACHWN2_003929"
     CDS             455..2071
                     /gene="bglH"
                     /locus_tag="ACHWN2_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR8933241.1"
                     /translation="MFRQNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            2098..3273
                     /locus_tag="ACHWN2_003930"
     CDS             2098..3273
                     /locus_tag="ACHWN2_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR8933242.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            3275..3997
                     /locus_tag="ACHWN2_003931"
     CDS             3275..3997
                     /locus_tag="ACHWN2_003931"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR8933243.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(4059..>4630)
                     /gene="cbrC"
                     /locus_tag="ACHWN2_003932"
     CDS             complement(4059..>4630)
                     /gene="cbrC"
                     /locus_tag="ACHWN2_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFR8933244.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
BASE COUNT         1283 a         1026 c         1131 g         1190 t
ORIGIN      
        1 cgggttctgt taaatacgct ttgggatcgt tatcaaaaac cgttatttat tgtcgagaat
       61 ggattaggcg taaaagacag cgttgaagcg gatggttcga tacaggacga ttatcgaatt
      121 gcctatttaa acgatcacct ggtacaggta aatgaagcga ttgccgatgg tgtggatatt
      181 atggggtaca ccagttgggg gccaattgat ttagtcagtg catctcattc acaaatgtct
      241 aagcgctacg gctttattta tgtggatcgt gatgataatg gcgaaggaag cctcacaaga
      301 acgcgtaaga aaagcttcgg atggtatgca gaagtgatca aaacgcgggg gctgtcatta
      361 aaaaaataac caataaagca ccttaattat cgtcgcattc agaacagtct ggatgcgata
      421 cgttaattct ttctttgcac cataaaggga tattatgttt agacaaaatc ttattacctc
      481 tgccatctta ttaatggcac cgttagcctt ttctgcacaa tcattggctg aatcattaac
      541 ggtggaacaa cgccttgagt tattagaaaa agcgttaaga gaaacgcaaa gcgaactcaa
      601 aaagtataaa gatgaagaga agaaaaaata tacgccagcg acggtgaatc gtagcgtaag
      661 tacgaatgat caagggtatg ccgccaatcc gttcccgacc agtagtgccg caaaacctga
      721 tgctgtactg gtcaaaaatg aagagaaaaa tgccagtgag acaggctcga tttattcttc
      781 catgactctg aaagatttca gtaagtttgt gaaagatgaa attggcttta gttacaacgg
      841 ctattaccgt tctggttggg ggaccgcctc tcatggttca cctaaatcat gggcgattgg
      901 ttctctgggc cgctttggta acgaatactc cggctggttt gatttgcagt taaaacaacg
      961 tgtctacaac gaaaacggca aacgggttga tgccgttgtg atgatagatg gtaacgttgg
     1021 tcagcagtac tctaccggct ggtttggcga taatgccggt ggcgagaact ttatgcagtt
     1081 ctccgatatg tacgttacca ccaaaggttt cctgcccttt gcgccagagg ctgatttctg
     1141 ggtgggtaaa cacggtgcgc cgaaaattga aatccagatg ctggactgga aaacgcagcg
     1201 tactgatgct gcagcgggtg taggtctgga aaactggaaa gtcggtccgg gtaaaattga
     1261 tatcgcgctg gttcgcgaag atgtcgatga ttacgatcgc agcctgcaaa acaaacagca
     1321 gattaatacc catactattg atttacgcta taaagatatc ccgttatggg ataaagcgac
     1381 cttaatggtg agtggtcgtt atgtcacggc aaacgaaagc gcatcggaaa aagataatca
     1441 ggataataac gggtattatg actggaaaga tacctggatg ttcggcacat ctttaacgca
     1501 gaaatttgat aaaggtggct tcaacgaatt ctccttcctg gttgcgaata actctatcgc
     1561 caggaacttt ggccgttatg ctggcgcaag tccatttacc acctttaatg gtcgttatta
     1621 tggtgatcac accggcggaa cagcagttcg tctgacctcg cagggcgaag cctatatcgg
     1681 cgatcatttt attgtggcta acgcgattgt ttactccttc ggtaacaata tttatagcta
     1741 cgaaacaggc gcacactctg atttcgaatc tattcgtgcg gttgttcgcc cggcctatat
     1801 ttgggaccaa tataaccaga caggtgttga actgggctat ttcacccagc aaaacaaaga
     1861 tgcgaatagt aataaattta atgagtctgg ttataaaacc acgctcttcc atacctttaa
     1921 agtcaatacc agtatgttga cctcgcgtct ggaaattcgt ttctacgcca cgtatatcaa
     1981 agccctggaa aacgaactgg atggcttcac cttcgaagac aataaagacg cccagtttgc
     2041 tgtcggtgcc caggctgaaa tctggtggta atggaactta atgaaaggaa tattacgatg
     2101 aatataaaaa ttgctgcttt aacgctggct attgccagcg gtatttctgc tcagtgggcc
     2161 atagcagcgg atatgccagc cagcccggca cccactatcc cggtgaaaca gtatgtgact
     2221 caggtcaatg ccgataacag cgtgaccttt cgctactttg cccctggggc aaaaaatgtc
     2281 tctgtagtgg tgggtgttcc ggttccggac aatattcacc cgatgaccaa agacgaagca
     2341 ggagtctggt cgtggcgcac acctatcctg aaaggcaacc tgtacgagta ttttttcaat
     2401 gttgatggtg tacgcagcat tgatacaggc accgcaatga ctaagcctca gcgccaggtt
     2461 aactccagta tgattctggt gccaggcagt tatctggata cgcgttctgt tgcgcatggt
     2521 gatttgatcg ccataactta ccactccaac gcattgcaat ctgaacgtca gatgtatgtc
     2581 tggaccccgc caggatacac cggcatgggc gagcctttgc cagtgctcta tttctatcac
     2641 ggttttggtg ataccggacg ttccgctatc gatcaggggc gtatcccgca aatcatggat
     2701 aacctgctgg ctgaagggaa aattaaaccg atgctggtgg tgatcccgga taccgaaacc
     2761 gatgcgaagg gcattattcc cgaagatttc gtgcctcagg aaagacgtaa agtcttttat
     2821 ccgctgaatg ctaaagcggc agatcgcgaa ctgatgaacg atattatccc gctgattagc
     2881 aagcgtttta gtgtccgtaa agatgccgat ggtcgcgcgc tggcagggct ttcacaaggc
     2941 gggtaccagg cgctggtttc cggaatgaat catctggaaa gctttggctg gctggccaca
     3001 ttcagtggtg ttaccacgac aaccgtaccg gatgaaggtg tcgcggcccg gctgaacgat
     3061 ccggcagcta ttaaccagca actacgtaat tttactgtgg tcgttggtga taaagatgtc
     3121 gtaaccggca aggatatcgc cgggctgaaa actgagcttg agcagaaaaa aattaagttt
     3181 gattaccagg aatatcccgg cctgaaccat gaaatggatg tctggcggcc tgcctatgca
     3241 gccttcgtac agaaatttaa aataaggcat taagatgaaa ttaatcatta ccgaagatta
     3301 ccaggaaatg agccgtgtcg cggcacacca tctgcttggt tatatgtcga agacgcgtcg
     3361 tgttaacctg gcaattaccg ccggtagcac gcccaaaggc atgtatgaat acctcaccac
     3421 cctggttaaa ggtaagcctt ggtacgataa ctgctatttc tataatttcg atgaaattcc
     3481 ctttcgcggc aaagagggag aaggcgtaac gattaccaat ctgcgtaatc tgtttttcac
     3541 ccctgcgggg atcaaagaag agaatatcca gaagctcact attgataact accgcgagca
     3601 tgatcagaaa ctggcgcgtg aaggcggcct ggatttggtg gtgctgggat taggtgcaga
     3661 tggtcatttt tgtggcaatt tgccgaatac gacccatttc catgagcaaa ccgtggagtt
     3721 cccgatccag ggagagatgg ttgatattgt ggcgcatggt gagttgggcg gcgacttttc
     3781 gttagtgcct gacagctacg tgacgatggg gccgaaaagc atcatggcgg cgaaaaactt
     3841 gctgataatt gtcagtggcg caggtaaagc gcaggcactg aaaaatgtgc tgcaaggtcc
     3901 ggttaccgaa gatgttccag cttccgtgtt gcagctgcat ccttcgctga tggtaatcgc
     3961 tgataaagcc gctgccgcag aattagcgtt cggttaagct gaagaaataa ggccggatgt
     4021 atgaattacg ctccggcctg ttctcattat ttaaataact acgaaaaatc accccacagt
     4081 ctcagcttgc cgcagtggag acagcggaag agataaccct gacaatcacc acccttttgc
     4141 aggcatttag ctagatcaga atttctaata ccgaaattct cacaatcttc ttcaaggttg
     4201 gcaaattcat cgaggcgatc ttttatatca ttccagccca catagccgat aaaagcacag
     4261 aaatcgccac aatgcgcaag ccagaattcc tgttgccatc catgataacc tggcgtgcgt
     4321 tcaaccaact ctttcagcat ttcatcagga tatgtgttct taataccggc aaattcgtcc
     4381 tcttcatcat actcaaattc aacaccttct atgctggcat catcctgaaa actacctgca
     4441 aacttttctg cagcagaacc gtccgcaata cacaacggac agagatgttc aacttcatca
     4501 acgcaataaa agggacccga gtaataaact gacgtctgtt gttcacagca atcgcactct
     4561 acggttttat cctgttcaaa tgcgcctgtt tccagtggct tgggatgata tttgaatgta
     4621 agcgccccga
//
LOCUS       DAZSNO010000232         4602 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZSNO010000232 DAZSNO010000000
VERSION     DAZSNO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.234"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="ACHWN2_003933"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="ACHWN2_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR8933245.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="ACHWN2_003934"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="ACHWN2_003934"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR8933246.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="ACHWN2_003935"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="ACHWN2_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR8933247.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4602)
                     /gene="efeU"
                     /locus_tag="ACHWN2_003936"
     CDS             complement(3929..>4602)
                     /gene="efeU"
                     /locus_tag="ACHWN2_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR8933248.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1116 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAZSNO010000233         4552 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZSNO010000233 DAZSNO010000000
VERSION     DAZSNO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.235"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            128..463
                     /gene="eutS"
                     /locus_tag="ACHWN2_003937"
     CDS             128..463
                     /gene="eutS"
                     /locus_tag="ACHWN2_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR8933249.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            476..955
                     /gene="eutP"
                     /locus_tag="ACHWN2_003938"
     CDS             476..955
                     /gene="eutP"
                     /locus_tag="ACHWN2_003938"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR8933250.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            930..2303
                     /gene="eutA"
                     /locus_tag="ACHWN2_003939"
     CDS             930..2303
                     /gene="eutA"
                     /locus_tag="ACHWN2_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR8933251.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            2822..3516
                     /locus_tag="ACHWN2_003940"
     CDS             join(2822..3068,3068..3516)
                     /locus_tag="ACHWN2_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8933252.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(3635..4444)
                     /gene="murI"
                     /locus_tag="ACHWN2_003941"
     CDS             complement(3635..4444)
                     /gene="murI"
                     /locus_tag="ACHWN2_003941"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR8933253.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
BASE COUNT         1187 a         1036 c         1123 g         1206 t
ORIGIN      
        1 cataacagta aatatctctc cattcttatt gatctacctc accttttaaa cgcgcttgcc
       61 gaattttgtt atttactctg acgaaaaatt gtcacgatac acgaaagttt ttcacaggcg
      121 gcgactcatg gataaagaac gcatcattca ggaatttgtg cccggcaaac aggtcacact
      181 ggcgcatctc atcgcgcatc ccggtgagga actggcgaag aaaatcggcg ttccggatgc
      241 gggcgcaatt ggcattatga cgctaactcc cggcgaaacg gcgatgatcg ccggtgattt
      301 agcgctgaaa gccgctgatg tgcatatcgg ttttctcgac cgcttcagcg gtgcattggt
      361 gatttatggc tcggtgggcg cagtagaaga agcgttatca caaaccgtca gcggtttagg
      421 tcgcttatta aattatacgc tttgtgaaat gaccaaaagt taatacgagg cattcatgaa
      481 acgaattgcg tttgtgggta gcgtcggggc aggaaaaaca acgttattta atgcgttgca
      541 ggggaattat accctcgcca gaaaaacact ggccgtggaa tttaatgata agggcgatat
      601 tgatacgccg ggtgaatatt ttagccatcc ccgctggtat cacgccttaa ttaccacgct
      661 acaggatgta gacatgctga tctatgtcca tggcgcaaat gacccggaaa gtcggttgcc
      721 tgccgggttg ctggatattg gcgtcagcaa acggcaaatc gccgtcatca gtaaaacgga
      781 catgccagat gctgacgtcg ccgcaacgcg aaagttattg cttgagacgg gtttcgaaga
      841 gccgattttc gagctaaaca gccacgaccc gcaaagcgtg cagcaactgg tggattatct
      901 ggcatctctc accaaacagg aggaagcagg tgaaaaaact catcacagcg aatgatattc
      961 gtgaagcaca cgcgcgcggc gaacaggcaa tgtccgtggt tctgcgcgcc agcattatta
     1021 ccccggaagc ccgtgaagtg gcggatctac tgggttttac catcaccgaa tgtgatgaat
     1081 cgatcccggt aacggcgtcc gtgcccgcca gcgtgccagc cgacaaaacc gaaagccagc
     1141 gcatccgcga aaccatcatt gcccagctgc cggaaggcca gtttaccgaa agcctggtcg
     1201 cacagctgat ggaaaaagtg atgaaggaaa aacagtcgct gggcgatttt gtcgtcgcca
     1261 gcgccgggcc gcacctcgaa tccgtgatcg ccggtcacgg agccggggcg caaacccttt
     1321 ctgaacaacg gctgtgtcgg gtactgaata tcgatatcgg cggtggcacc gcgaactacg
     1381 ccctgttcga tgccggaaaa atcagcggca ctgcctgtct caacgtcggt ggtcgcctgc
     1441 tggaaaccga cagccagggg cgcgtggttt acgctcataa accggggcag atgattgtgg
     1501 atgagtgctt cggtgcaggc actgacgccc gttcgctgac cggcgcgcag ctggtgcagg
     1561 ttacccggcg gatggcggcg ctgattgtcg aagtgattga cggaacgctt tcgccgctcg
     1621 cgcaggcatt gatgcaaacc ggtttgctgc ccgcaggtgt tacgcccgaa atcattacgc
     1681 tttctggagg cgtgggcgaa tgttatcgcc accagcccgc caacccgttc tgttttgccg
     1741 atattggccc acttctggca acggcgctgc atgaccatcc gcgcctgcgt gagatgaatg
     1801 tgcagtttcc ggcgcaaacc gtacgctcca cggtgattgg cgcgggtgca catacccttt
     1861 cgctctctgg cagcacaatc tggctggagg gcgtacaact gccgctgcgc aatttgccgg
     1921 tggcgatccc gattgatgaa acggatctgg tgagtgcctg gcaacaggcg ctgcttcagc
     1981 tggatcttga tcccaaaact gacgtgtacg tgctggcgct tcccgcctcg ctgcctgtgc
     2041 gttacgccgc ggttctgacg gtcatcaacg cgctggtcga tttcgtcgcg cgttttccga
     2101 atccgcatcc cctgctggtg gtggccgggc aggactttgg taaagctctg ggcatgttgt
     2161 tgcgcccaca gctacaacaa ctcccgttgg cagtcattga cgaagtgatt gtccgcgcgg
     2221 gggactatat cgacattggt acgcctcttt ttggcggatc ggttgtgccg gtgacggtga
     2281 aatcactcgc atttccttcc tgaatacggt agttatcagg tatggtgctt ggtgtctggg
     2341 tgttagtggg attatatccg gcagtgtatc cggttaaggt gattggtctg gcaagatgat
     2401 tggatgcttt tgtggtgaaa aatgttttgg tgttttagca taaccctgaa gaaagaataa
     2461 aaataaaaag gaaaaaacca aaaccagaac agaaccggaa agagaagaaa agagaaaatg
     2521 aaaaataaaa accaaaaaag aaagaatact taaatcttcg catgttcttc gggtggtacg
     2581 caagtttgtt agtctgaccg cacaataaat tttcgcttag aaaataataa gttacataat
     2641 attctgcttt tttagataaa tataacccga aaaaatcatt gttaatctta tgcatttatt
     2701 atttgattgt aagttgtgat tattataatt tagttattgt tagtatgttt tataatgctg
     2761 gatgggtgat gcctctaatt agttgaatct gatgtataat acgggctttt gaggttatct
     2821 catgaccagc gttaatattc attgtccccg ttgtcagtca gctcaggttt accgccatgg
     2881 tcagaaccct aaaggccgtg acagacttcg ctgccgtgac tgccaccgtg tgtttcagtt
     2941 cacttatact tatcaagcac gtaagccggg tatgaaagag ctgataactg aaatggcctt
     3001 taatggtgcc ggggttcgcg ataccgccag gacactgaaa attggtagta acaccgtcat
     3061 ccggacttta aaaaactcgc gccaaagcga ataacttctt cgcctgttgc ccatgctgat
     3121 gtggcgttta tctgcgagct tgatgagcaa tggagctacg ttggcagtaa agcccggcaa
     3181 cattggctcg ggtacgcgta caacaccaaa acaggcggtg tactggccta cacttttggt
     3241 ccccgaaccg atcaaacgtg ccgggagcta ctggcactgc ttacaccctt caacatcggc
     3301 atgctgacca gcgatgactg gggcagctat ggccgggagg tgccgaagaa taagcatctg
     3361 accggcaaaa tattcaccca acgcattgag cgtaataatc tgacgctacg cacccgcatt
     3421 aagcggttgg gtcgtaaaac aatctgcttc tcacgctcag ttgagatcca cgaaaaagtg
     3481 atcggagcgt ttatcgaaaa acacatgttc tactaattgg atgcactacc cagtatgtaa
     3541 ctgggtttgt ccataaaatg caccttactc agttggtgtc caacttttgg ggtgcagttc
     3601 agaaggacgg gattttacgg tgtaccggat aaatttaaaa ctgcctgata ctgaatgtat
     3661 gaggtttcgt aatgctcaat tcccctagta ccgttttgta gatctccata cttccactag
     3721 tatttatttg caaagttcct atatttgagg agtttaataa atttagctgt cccaagtgat
     3781 ttttaataga gatagcctga tcatgtgcag gattaataaa ttttatatcc ggtgcaactg
     3841 cttcaaaaaa attctgaaca attggatagt gagtacagcc gagaataatt tcatttactg
     3901 gatggaggga aattaaatgg ttaacatgtt ttttgatgtc attcaagatt gcaggagcat
     3961 taagattacc ttcctcaatt aacattgcaa gatttttgct tggttctcca aatacattta
     4021 ttgttgaatt caattttttt attagctctt tgtgacaccc cgttttaata gtaaactcgg
     4081 tagcgataat accaacattt ttatattgct ggcgaatcac atcttctact gcaggttcaa
     4141 tgatggataa gataggaaac ggaaagctct tagcatatcc gctgtattct tcggattcca
     4201 aagttgagga tatagtatta catgcgacag caactagttt gacattcctt gactgtaaaa
     4261 aagtcagcat ttttttagtc aaactgatga tatcatcggc agaacggttg ccataaggtg
     4321 cattcatatt atctccacaa tagataatat cttcaccagg aagcaaatta attatttcct
     4381 ttaccgatgt caatccaccg acacctgagt ctattacacc aattggagat tcagcactaa
     4441 acatatttaa cctcatcttt tgtagcttat ggaaaattta attactagtt cttttttccc
     4501 aagaaaatgg ttcgaaatca gagtttggat ctttccagtc agattttcgg aa
//
LOCUS       DAZSNO010000234         4442 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZSNO010000234 DAZSNO010000000
VERSION     DAZSNO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.236"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            156..1013
                     /gene="focA"
                     /locus_tag="ACHWN2_003942"
     CDS             156..1013
                     /gene="focA"
                     /locus_tag="ACHWN2_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR8933254.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1068..3350
                     /gene="pflB"
                     /locus_tag="ACHWN2_003943"
     CDS             1068..3350
                     /gene="pflB"
                     /locus_tag="ACHWN2_003943"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR8933255.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3542..4282
                     /gene="pflA"
                     /locus_tag="ACHWN2_003944"
     CDS             3542..4282
                     /gene="pflA"
                     /locus_tag="ACHWN2_003944"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR8933256.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4364..>4442)
                     /locus_tag="ACHWN2_003945"
     CDS             complement(4364..>4442)
                     /locus_tag="ACHWN2_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933257.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT         1128 a         1101 c         1080 g         1133 t
ORIGIN      
        1 atttttattt ggataatcaa atagttactc cgtatttgca taaaaaccat gcgagttacg
       61 ggcctataag ccaggcgaga tatgatctat atcaatttct catctataat gctttgttag
      121 tatctcgtcg ccgacttaat aaagagagag ttagtgtgaa agctgacaac ccttttgatc
      181 ttttacttcc tgctgcaatg gccaaagtgg ccgaagaggc gggtgtctat aaagcaacga
      241 aacatccgct taagactttc tatctggcga ttaccgccgg tgttttcatc tcaatcgcat
      301 tcgtcttcta tatcacagca accactggca caggcacaat gcccttcggc atggcaaaac
      361 tggttggcgg catttgcttc tctctggggc tgattctttg tgttgtctgc ggagccgatc
      421 tctttacttc cacagtgttg attgttgttg ctaaggcgag tgggcgcatc acctggtgtc
      481 agttggctaa aaactggcta aatgtctatt ttggcaacct ggtcggcgca ctgctgtttg
      541 tacttttaat gtggctttcc ggcgagtata tgaccgcaaa tggtcaatgg ggactaaacg
      601 tcctacaaac cgccgaccac aaagtgcacc atacttttat tgaggccgtc tgtcttggta
      661 tcctggcaaa cctgatggta tgtctggcag tatggatgag ttattctggc cgcagcctga
      721 tggacaaagc gttcattatg gtgctgccgg tcgcgatgtt tgttgccagc ggttttgagc
      781 acagtatcgc aaacatgttt atgatcccga tgggtattgt aatccgcgac ttcgcatctc
      841 cggaattctg gaccgctgtc ggttctgcac cggaaaattt ttctcacctg accgtgatga
      901 acttcatcac tgataacctg atcccggtta cgatcggtaa tattatcggc ggtggtttgt
      961 tggttgggtt gacatactgg gtcatttacc tgcgtgaaaa cgatcaccat taatggttgt
     1021 cgaagtacgc agtaaataaa aaatccactt aagaaggtag gtgttacatg tccgagctta
     1081 atgaaaagtt agccacagcc tgggaaggtt ttaccaaagg tgactggcag aatgaagtaa
     1141 acgtccgtga cttcattcag aaaaactaca ctccgtacga gggtgacgag tccttcctgg
     1201 ctggcgctac tgaagcgacc accaccctgt gggacaaagt aatggaaggt gttaaactgg
     1261 aaaaccgcac tcacgcgcca gttgattttg acaccgctgt tgcttccacc atcacctctc
     1321 acgacgctgg ctacatcaat aaagcgttgg aaaaagttgt tggtctgcag actgaagctc
     1381 cgctgaaacg tgctcttatc ccgttcggtg gtatcaaaat gatcgaaggt tcctgcaaag
     1441 cgtacaaccg cgaactggac ccgatgatca aaaaaatctt cactgaatac cgtaaaactc
     1501 acaaccaggg cgtgttcgac gtttacactc cggacatcct gcgttgccgt aaatccggtg
     1561 ttctgaccgg tctgccagat gcttatggcc gtggccgtat catcggtgac taccgtcgcg
     1621 ttgcgctgta cggtatcgac tacctgatga aagacaaata cgctcagttc acctctctac
     1681 aggctgatct ggaaaacggc gtaaacctgg aacagactat ccgtctgcgc gaagaaatcg
     1741 ctgaacagca ccgcgctctg ggtcagatga aagaaatggc tgcgaaatac ggctacgaca
     1801 tctctggtcc ggctaccaac gctcaggaag ctatccagtg gacttacttc ggctacctgg
     1861 ctgctgttaa gtctcagaac ggtgctgcaa tgtccttcgg tcgtacctcc accttcctgg
     1921 atgtgtacat cgaacgtgac ctgaaagctg gcaagatcac cgaacaagaa gcgcaggaaa
     1981 tggttgacca cctggtcatg aaactgcgta tggttcgctt cctgcgtact ccggaatacg
     2041 atgaactgtt ctctggcgac ccaatctggg caaccgaatc tatcggtggt atgggcctcg
     2101 atggtcgtac cctggttacc aaaaacagct tccgtttcct gaacaccctg tacactatgg
     2161 ggccgtctcc ggaaccgaac atgaccattc tgtggtctga aaaactgccg ctgaacttca
     2221 agaaattcgc cgctaaagtg tccatcgaca cctcttctct gcagtatgag aacgatgacc
     2281 tgatgcgtcc ggacttcaac aacgatgact acgctatcgc ttgctgcgta agcccgatga
     2341 tcgttggtaa acaaatgcag ttcttcggtg cgcgtgcaaa cctggcgaaa accatgctgt
     2401 acgcaatcaa cggcggcgtt gacgaaaaac tgaaaatgca ggttggtccg aagtctgaac
     2461 cgatcaaagg cgatctcctg agctacgatg aagtgatgga gcgcatggat cacttcatgg
     2521 actggctggc taaacagtac atcactgcac tgaacatcat ccactacatg cacgacaagt
     2581 acagctacga agcctctctg atggcgctgc acgaccgtga cgttatccgc accatggcgt
     2641 gtggtatcgc tggtctgtcc gttgctgctg actccctgtc tgcaatcaaa tatgcgaaag
     2701 ttaaaccgat tcgtgacgaa gacggtctgg ctatcgactt cgaaatcgaa ggcgaatacc
     2761 cgcagtttgg taacaatgat ccgcgtgtag atgacctggc tgttgacctg gtagaacgtt
     2821 tcatgaagaa aattcagaaa ctgcacacct accgtgacgc tatcccgact cagtctgttc
     2881 tgaccatcac ttctaacgtt gtgtatggta agaaaactgg taacacccca gacggtcgtc
     2941 gtgctggcgc gccgttcgga ccgggtgcta acccgatgca cggtcgtgac cagaaaggtg
     3001 ctgtagcgtc tctgacttcc gttgctaaac tgccgtttgc ttacgctaaa gatggtatct
     3061 cctacacctt ctctatcgtt ccgaacgcac tgggtaaaga cgacgaagtt cgtaagacca
     3121 acctggctgg tctgatggat ggttacttcc accacgaagc atccatcgaa ggtggtcagc
     3181 acctgaacgt taacgtgatg aaccgtgaaa tgctgctcga cgcgatggaa aacccggaaa
     3241 aatatccgca gctgaccatc cgtgtatctg gctacgcagt acgtttcaac tcgctgacta
     3301 aagaacagca gcaggacgtt attactcgta ccttcactca atctatgtaa ttagatttga
     3361 ctgaaatcgt acagtaaaaa gcgtacaata aaggctccac gaaagtgggg ccttttttag
     3421 cacgagagcc ttttttgtca gctatctata ctttaaggtg actgccaaaa cagactcgac
     3481 gtagccttcg agctgcgcac caacacggcc tcagatgggc cacatctgga gaaacaccgc
     3541 aatgtcagtt attggtcgca ttcactcctt tgaatcctgt ggaaccgtag acggcccagg
     3601 tattcgcttt atcacctttt tccagggctg cctgatgcgc tgcctgtatt gtcataaccg
     3661 cgacacctgg gacacgcatg gcggtaaaga agttaccgtt gaagatttga tgaaggaagt
     3721 ggtgacctat cgccacttta tgaacgcttc cggcggcggc gttaccgcat ccggcggtga
     3781 agcaatcctg caagctgagt ttgttcgtga ctggttccgc gcctgtaaaa aagaaggcat
     3841 tcatacctgt ctggatacca acggttttgt tcgtcgttac gatccggtga ttgatgaact
     3901 gctggaagta accgacctgg taatgctcga tctcaaacag atgaacgacg agatccacca
     3961 aaatctggtt ggagtttcca accaccgcac gctggagttc gctaaatatc tggcgaacaa
     4021 aaatgtgaag gtgtggatcc gctacgttgt tgtcccaggc tggtctgacg atgacgattc
     4081 ggcacatcgc ctcggtgaat ttacccgtga tatgggtaac gttgagaaaa tcgaacttct
     4141 cccctaccac gaactgggca aacacaaatg ggtggcgatg ggtgaagaat acaaactcga
     4201 tggtgttaaa ccaccgaaga aagagaccat ggaacgcgtg aaaggcattc ttgagcagta
     4261 cggtcataag gtaatgttct aatttcctcg taaagcggca actttattga gttgccgctt
     4321 ttttattccg cgcttcattc tgctacagcg tttttaacac atcttatagt gcatctacca
     4381 tatcccgtac ctgagataat aacgactgat aatagctcgc gtgaagttct tccccatcga
     4441 at
//
LOCUS       DAZSNO010000235         4320 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZSNO010000235 DAZSNO010000000
VERSION     DAZSNO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.238"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..320)
                     /locus_tag="ACHWN2_003946"
     CDS             complement(<1..320)
                     /locus_tag="ACHWN2_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR8933258.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVA"
     gene            complement(435..510)
                     /locus_tag="ACHWN2_003947"
     tRNA            complement(435..510)
                     /locus_tag="ACHWN2_003947"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(475..477),aa:Thr,seq:ggt)
     gene            complement(517..591)
                     /locus_tag="ACHWN2_003948"
     tRNA            complement(517..591)
                     /locus_tag="ACHWN2_003948"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(556..558),aa:Gly,seq:tcc)
     gene            complement(708..792)
                     /locus_tag="ACHWN2_003949"
     tRNA            complement(708..792)
                     /locus_tag="ACHWN2_003949"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(756..758),aa:Tyr,seq:gta)
     gene            complement(801..876)
                     /locus_tag="ACHWN2_003950"
     tRNA            complement(801..876)
                     /locus_tag="ACHWN2_003950"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(841..843),aa:Thr,seq:tgt)
     gene            1238..2188
                     /gene="coaA"
                     /locus_tag="ACHWN2_003951"
     CDS             1238..2188
                     /gene="coaA"
                     /locus_tag="ACHWN2_003951"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR8933259.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2217..3182)
                     /gene="birA"
                     /locus_tag="ACHWN2_003952"
     CDS             complement(2217..3182)
                     /gene="birA"
                     /locus_tag="ACHWN2_003952"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR8933260.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3179..4207)
                     /gene="murB"
                     /locus_tag="ACHWN2_003953"
     CDS             complement(3179..4207)
                     /gene="murB"
                     /locus_tag="ACHWN2_003953"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR8933261.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1108 a         1076 c          974 g         1162 t
ORIGIN      
        1 gcaactacca ggatcgcgcc gtccatctga gcagcaccgg tgatcatgtt tttaacatag
       61 tcggcgtgcc ccgggcagtc tacgtgtgcg tagtgacggg tcggggtgtc gtattcaacg
      121 tgagaagtgt tgatggtgat accacgagct ttttcttccg gcgcgttatc gatctggtcg
      181 aatgcgcgag cagcaccgcc gtaggtttta gccagtacgg tagtgattgc agcggtcagc
      241 gttgttttac catggtcaac gtggccgata gtaccgacgt taacgtgcgg ttttgtacgt
      301 tcaaactttt ctttagacat cgattgtccc tctaagacac ggataaatcg gtgatatcac
      361 cacatcaacc aggcaacatg cccgacttgt tgaatgcaat aaacagaagg aaaaaacagg
      421 gaggagaaaa gaagtggtgc tgataggcag attcgaactg ccgacctcac ccttaccaag
      481 ggtgcgctct accaactgag ctatatcagc acatcttgga gcgggcagcg ggaatcgaac
      541 ccgcatcatc agcttggaag gctgaggtaa tagccattat acgatgcccg catcctagaa
      601 ctcggctacc tgattttcat tctgcactga atatcgagag aagctctctt tattcgagcc
      661 ggtaagtgaa cttatcgtct cgggctacgc catcgcgtgg ccgaaattgg tggtggggga
      721 aggattcgaa ccttcgaagt ctgtgacggc agatttacag tctgctccct ttggccgctc
      781 gggaacccca ccggacttga tggtgccgac taccggaatc gaactggtga cctactgatt
      841 acaagtcagt tgctctacct actgagctaa gtcggcatca agtagcgcgc attctatgga
      901 gacatgcgag ttcatgcaac taaaaaattg cataatttgt tttattggtc acattttatg
      961 ctacacgatg aagaaacagc cgaacggagg gcagattagc acactttttc aacatcattg
     1021 tgctcaacaa tgcgctcctg ctaaaccata attcttttta tcagatggaa tatctgtcac
     1081 attgcttttc aacgatagct tcctggcaga gattttttct tattattcct ccccatctgg
     1141 tgttaccctc ctgcccatta acccattcaa cagaactgtg acgcgccatg gcaaatatcg
     1201 ctttgccgat agagctatga ccgccagaaa catgcttatg agtataaaag agcaaacgtt
     1261 aatgacgcct tacctacagt ttgaccgcaa ccagtgggca gctctgcgtg attccgtacc
     1321 tatgacgtta tcggaagatg agatcgcccg tctcaaaggt attaatgaag atctctcgtt
     1381 agaagaagtt gccgagatct atttaccttt gtcacgtttg ctgaacttct atataagctc
     1441 gaatctgcgc cgtcaggcag ttctggaaca gtttcttggt accaacgggc aacgcattcc
     1501 ttacattatc agtattgctg gcagtgtcgc ggtggggaaa agtacaaccg cccgtgtatt
     1561 gcaggcgcta ttaagccgtt ggccggaaca tcgtcgtgtt gaactgatca ctacagatgg
     1621 cttccttcac cctaatcagg ttctgaaaga acgtggtctg atgaagaaga aaggcttccc
     1681 ggaatcgtat gatatgcatc gcctggtgaa gtttgtttcc gatctcaaat ccggcgtgcc
     1741 aaacgttaca gcccctgttt actcgcatct tatttatgat gtgatcccgg atggagataa
     1801 aactgttgtt cagcctgata ttttaattct tgaagggtta aatgtcttac agagcgggat
     1861 ggattatcca cacgatccac atcatgtatt tgtttccgat tttgtcgatt tttcgatata
     1921 tgttgatgca ccggaagact tacttcagac gtggtatatc aaccgttttc tgaaattccg
     1981 cgaaggggct tttacagacc cggattccta ttttcataac tacgcgaaat taactaaaga
     2041 agaagcgatt aagactgcca tgacattgtg gaaagagatc aactggctga acttaaagca
     2101 aaatattcta cctactcgtg agcgcgccag tttaatcctg acgaaaagtg ctaatcatgt
     2161 ggtagaagag gtcagactac gcaaataatt tgcaggggag cgaatactcc cctttcttat
     2221 ttttctgcac tacgcaggga tatttcaccg cccatccagg gttttattat tccatcctgc
     2281 tcaagtaata aagccccctg tttgtctatt ccgcgtgaaa tgccaaatat ttctttatca
     2341 ccaatgataa gtttcactgg gcgattaata aaattatcca gcttttccca gcgcgacaga
     2401 taaggtgcca atccttcttg ttcgaagagt tccaacgcag cacgtaattc acgtattagc
     2461 atggccgcca acgtattacg atcaagattg atccccgctt cctgcagcgt gatccacccc
     2521 tgattaacga cactctcttc aacacggcgc attgccatgt tgatcccggc tccaatgact
     2581 atttgcgccg catcgccagt tttgccagtc agctccacaa gaatgcctgc cagcttgcga
     2641 tcctgcagat agaggtcatt aggccatttg acacgaactt tatctgctcc cagctttcgt
     2701 aatacttccg ccatcacgat accgataacc agacttaaac caatcgccgc cgccgggcct
     2761 tgttccagac gccagaacat cgacaaatat aagtttgcgc caaaaggcga aaaccatttc
     2821 cgcccccggc gaccacggcc agcctgctgg tattctgcaa cacaggcatc gcccgattta
     2881 agctctccga tacgatcaag aaggaactga ttcgtggagt caataactgg cagcacggct
     2941 acactaccgc catccagctg acccaatatc tgttcagcat taagtaactg gatgggctca
     3001 ggcaggctgt atcctttacc cggaacggta aagacatcaa cgccccagtc acgcagtgtc
     3061 tgaatgtgtt tattaatagc cgcccggctc attcccagcg tttcacccaa ctgctcgcca
     3121 gagtgaaatt caccgttcgc taacagggca atcaatttca gtggcacggt gttatccttc
     3181 atgaaattgt ctccactgcg ctcacttcac ctgatgcacc aataaagcgg acttcaggct
     3241 caagccagac attaaatttt tcacccactt tttgtcttac atggtgtgcc agttgcacca
     3301 catcttcgct ttttgcattg tcttcattaa tgagaactaa cgcctgttga cggtgcaccg
     3361 cagccccacc catttgcatc ccttttagct ggcactgatc aataagccaa cctgctgcca
     3421 gttttactga accacccgcc tgggggtaat ttggtgctgt tggaaattgc gccagtaatg
     3481 ctttagccgt ttcggcagat acaacagggt ttttgaagaa actaccggca ttgccattca
     3541 cttttggatc agggagtttg gtggtgcgca tatgacacac cgcattaaat acttgctgtg
     3601 gcgttactgt tgtaggatcc agacgagtta agtcaccata cgttagtaca ggttgccact
     3661 cttttggcag acgcagacct acggctacaa tggcgaagcg atcctggtat tcatgtttaa
     3721 aaatactgtc gcgatagcca aaacggcact ctttggcagt taagcgcact tgcttgcctg
     3781 tcgccagttc aacacaatca acataagcgc aaactcgctg taattctacg ccataagcac
     3841 caatattctg gataggtgat gagccgacac aaccaggaat taatgccaga ttttccagac
     3901 caggcatacc ttcctgcaaa gtgtatttta ccaggcgatg ccagttttct ccggctccta
     3961 catgtaaata ccacgcatca ggttcatcat gaatttcgat acctttgatc cggttgatga
     4021 tcaccgtgcc gcgatagtct tccagaaaaa gtacattact tccttcaccc agaataagaa
     4081 cgggttgtcc ttctgcggtt gcatgctgcc aggcattgag taattgttgt tcgtcttcgg
     4141 cacatacaat gtgctgagca ttatgatcaa tgccaaatgt gttccagggt tttaaggagt
     4201 ggttcatagc tgctttcctg atgcaaaaac gaggctagtt taccgtatct gtggggggat
     4261 ggcttgtaga tatgacgaca ggaagagttt gtagaaacgc aaaaaggccc gactgggctt
//
LOCUS       DAZSNO010000236         4240 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZSNO010000236 DAZSNO010000000
VERSION     DAZSNO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.239"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(125..871)
                     /gene="cutC"
                     /locus_tag="ACHWN2_003954"
     CDS             complement(125..871)
                     /gene="cutC"
                     /locus_tag="ACHWN2_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR8933262.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(885..1451)
                     /locus_tag="ACHWN2_003955"
     CDS             complement(885..1451)
                     /locus_tag="ACHWN2_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR8933263.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1667..3400
                     /gene="argS"
                     /locus_tag="ACHWN2_003956"
     CDS             1667..3400
                     /gene="argS"
                     /locus_tag="ACHWN2_003956"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR8933264.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3442..3486)
                     /locus_tag="ACHWN2_003957"
     CDS             complement(3442..3486)
                     /locus_tag="ACHWN2_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933265.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3577..4065
                     /locus_tag="ACHWN2_003958"
     CDS             3577..4065
                     /locus_tag="ACHWN2_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR8933266.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         1118 a         1049 c         1035 g         1038 t
ORIGIN      
        1 tcaacagatc acggttatcg ttcgtttttt atactgctca gggataaaaa aaggcccctg
       61 ttgaaattgc aggggcctgg tacgagcaag catcatattg ggcgacatga tgcaacggta
      121 aaaatcattt ggcctgatgg cgttcaatga ttcctttcat ttcagcaacc gccgccccgt
      181 ctacgatata acgcgaatac tcgtccgcgt gttcatctga tgacatggac aatccttgat
      241 tacgataacg catcggtgag gcttgccacg ctcccgcgga gctatggact tccagcactc
      301 cggcatcgag gaagtggtgc aggttttctg cacggactcc tgctccagcc ataatgattg
      361 gagcatcacg atgggcaata agttccataa tttttgataa accttgcagt gcgtctgatt
      421 tttgccctga tgtcagtacc cgggcaatgc ccaattccgc aagattattg agtgtatata
      481 aagggttagc gcacatatcg aaggcgcgat gaaaagtcac tgccagcgga ccggcagcag
      541 ccattatttt ttccattcgt ggcatatcga cattcccgtc aacatcgaga acgcccgtca
      601 ccagtccagg aaaacctaat tcgcggaccg tgcgcacatc ctcaagaatg gcggcaaact
      661 caccgtcgct gtagcaaaaa tcaccaccgc gtgggcgaat tatcggatgc acagggatcg
      721 tcacccgctg gcgcacggat ttcagtaccc ccaacgacgg cgttaagccc ccctcttttg
      781 gggctgcgca taattcaact ctgtctgcgc cgttttgctg cgccgttagt gcacattcca
      841 tgctgtaaca gcaaatttcc agtaatgcca tttttgctcc ttaattacgc tgactgctcg
      901 ctggcaacga tctcttcaat ggaccacgga tgaaacttaa tagttgtttt gccatcggtg
      961 accgccagcg tcggattcgg taatcgctca tgctcacctt ttggtgaact ggttttaact
     1021 gagatccccg gtaggctgag tccttcatca gaaagcaatg ccagcgcacg ggcattcagc
     1081 gtttctggat cacccggcag aacgatttca atatgttccc aaccttcgtg tgggtaacgt
     1141 ttttccccgg gccacggtag ctccacattg gaaaactgcc agtgcgcaac ctgtaccggt
     1201 tcatgcaatt taaacagaca aatcggtctg ccattgatca ttttttctga caaaagctcg
     1261 ccacactgtt caaaaccgcg acgccagcgc tcagcggtga cgttttgatg gcaacgcaaa
     1321 gaaatgtgat cggcagtcag cggagtgata tccaacccca gacggcggga aagttcatct
     1381 aatgcgtggg taaatcgcgg taaatccgat gcaatatcct gcagctcgtc gatagattgc
     1441 cagttcgcca taatcactct tcgtctttca gtaaaagcgt taatttaccc tgttgccctg
     1501 tgccaaccaa ccgctgattt cacgccgctt ctgatgcaat agtgaaaacg gcaatacgcc
     1561 acgcgcacgt tgctgacgaa aacagccatt tgcagtatac tcccgcccta atttctttaa
     1621 ctggtgcggg caatttttgc tcgcttcatc aatgtaaggt attccggtga atattcaggc
     1681 tcttctctca gaaaaagtcc gtcaggccat gattgcggca ggcgcgcctg cggattgcga
     1741 accgcagatt cgtcagtcag caaaagttca gttcggcgac tatcaggcta acggcatgat
     1801 ggcagttgct aaaaaactgg gtatggcacc gcgacaatta gcagagcagg tgctgactca
     1861 tctggatctt aacggtatcg ccagcaaagt tgagatcgcc ggtccaggct ttatcaacat
     1921 tttccttgat ccggcattcc tggctgaaca tgttcagcag gcgctggcgt ccgatcgtct
     1981 cggtgttgct acgccagaaa aacagaccat tgtggttgac tactctgcgc caaacgtggc
     2041 gaaagagatg cacgtcggtc acctgcgctc taccattatt ggtgacacag cagtgcgtac
     2101 tctggagttc ctcggtcaca aagtgattcg cgcaaaccac gtcggcgact ggggcactca
     2161 gttcggtatg ctgattgcat ggctggaaaa gcagcagcag gaaaacgccg gtgaaatgga
     2221 gctggctgac cttgaaggtt tctaccgcga tgcgaaaaag cattacgacg aagatgaaga
     2281 gtttgccgag cgtgcacgta actacgtggt aaaactgcaa agcggtgacg aatatttccg
     2341 cgagatgtgg cgcaaactgg tcgacatcac catgacgcag aaccagatca cttacgatcg
     2401 tctcaacgtg acgctgaccc gtgatgacgt gatgggcgaa agcctctaca acccaatgct
     2461 gccaggaatt gtggcggatc tcaaagccaa aggtctggca gtagaaagcg aaggggcgac
     2521 cgtcgtattc cttgatgtgt ttaaaaacaa ggaaggcgaa ccgatgggcg tgatcattca
     2581 gaagaaagat ggcggctatc tctacaccac cactgatatc gcctgtgcga aatatcgtta
     2641 tgaaacactg catgccgatc gcgtgctgta ttacatcgac tcccgtcagc atcaacacct
     2701 gatgcaggca tgggcgatcg tccgtaaagc aggctatgta ccggaatccg taccgctgga
     2761 acaccacatg ttcggcatga tgctgggtaa agacggcaaa ccgttcaaaa cccgcgcggg
     2821 tggtaccgtg aaactggcag acctgctgga tgaagccctg gagcgtgcac gtcgtctggt
     2881 ggcagaaaag aacccggata tgccagctga cgagctggaa aaactggcta acgcggttgg
     2941 tattggtgcg gtgaaatatg cggatctctc caaaaaccgc accacggact acatcttcga
     3001 ctgggacaac atgctggcgt ttgagggtaa taccgcgcca tacatgcagt atgcttacac
     3061 gcgtgtattg tccgtgttcc gtaaagcaga aattaacgaa gagcaactgg ctgcagctcc
     3121 ggtaatcatc cgtgaagatc gtgaagcgca actggcagct cgcctgctgc agtttgaaga
     3181 aaccctcacc gtggttgccc gtgaaggcac gccgcatgta atgtgtgctt acctgtacga
     3241 tctggccggt ctgttctctg gcttctacga gcactgcccg atcctcagcg cagaaaacga
     3301 agaagtgcgt aacagccgtc tgaaactggc acaactgaca gcgaagacgc tgaagctggg
     3361 tctggatacg ctgggtattg agactgtaga gcgtatgtaa tcgatttttc gtgagagtga
     3421 agcctgatca gggttagccg atcaggcttt tttattgcca tctaaatgta ttctgaaaac
     3481 ggacatgcca ttgttttctc actgttgagt aagaggccag aagcgtaata tccaggccca
     3541 ggaaaacgat aacggttcaa tttaaggaat accgaagtgt ttaaatttct tgtattaaca
     3601 ttaggcatta tctcttgcca ggcttacgca gaagatacgg ttatagtaaa cgaccatgac
     3661 acttcagcca tcaaagattg ttggcaaaaa aattcagatg atgatactga cattaacgtg
     3721 atcaaatcat gcctgcgaca agaatacaat ctcgtcgatg cgcaattaaa taaagcctat
     3781 ggtgaagctt atcgttatat agaacaagtg ccacgcacag gtgcaaaaaa acttgatact
     3841 gaacaactta acttgcttaa aaaatcacag cgagcctggc tggattttag ggacaaagaa
     3901 tgtgaattaa tcctttcaaa tgaggatgtt caggatttaa gtgaccccta ttctgaatca
     3961 gaatggctat catgtatgat catacagacc aatacgcgaa ctcgccagtt gcagctatac
     4021 cgtaactctg aagattttta tccaagccct ttgacaagag gataattcac atctttttgg
     4081 catgttttgt tgcaagctat tcctgataaa tgattgcaac aaaacatcca gccttttgac
     4141 tgagttatta aacatactcg cgagcgcgta attttttgtc tttcagcgat aattcacaat
     4201 cacttcattc cgttgtacct ttagcggtgg aattaaccgc
//
LOCUS       DAZSNO010000237         4221 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZSNO010000237 DAZSNO010000000
VERSION     DAZSNO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.240"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACHWN2_003959"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACHWN2_003959"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR8933267.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACHWN2_003960"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACHWN2_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR8933268.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACHWN2_003961"
     CDS             complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACHWN2_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR8933269.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACHWN2_003962"
     CDS             complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACHWN2_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR8933270.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="ACHWN2_003963"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="ACHWN2_003963"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR8933271.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1078 a         1138 c         1021 g          984 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata t
//
LOCUS       DAZSNO010000238         4211 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZSNO010000238 DAZSNO010000000
VERSION     DAZSNO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.241"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..437
                     /gene="tnpA"
                     /locus_tag="ACHWN2_003964"
                     /pseudo
     CDS             1..437
                     /gene="tnpA"
                     /locus_tag="ACHWN2_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(555..1493)
                     /gene="hypT"
                     /locus_tag="ACHWN2_003965"
                     /gene_synonym="qseD"
     CDS             complement(555..1493)
                     /gene="hypT"
                     /locus_tag="ACHWN2_003965"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFR8933272.1"
                     /translation="MKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKALEA
                     AIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAAAH
                     SLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQSPF
                     CCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGITG
                     RTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIYRM
                     NTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1844..2563
                     /locus_tag="ACHWN2_003966"
     CDS             1844..2563
                     /locus_tag="ACHWN2_003966"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR8933273.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2629..3927
                     /locus_tag="ACHWN2_003967"
     CDS             2629..3927
                     /locus_tag="ACHWN2_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HFR8933274.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1060 a          913 c         1036 g         1202 t
ORIGIN      
        1 gcacacccga tggaactgta aatatcacat agttttttgc gccaaaatac cgaagacagg
       61 tgttctacag agagaagcgt agagcaacag gcagtatttt gagaaagctg tgtgagtgga
      121 aaagcgtacg gattctggaa gctgaatgct gtgcagatca tatccatatg cttgtggaga
      181 tcccgcccaa aatgagcgta tccggcttta tgggatatct gaaagggaaa agcagtctga
      241 tgctttacga gcagtttggt gatttgaaat tcaaatacag gaacagggag ttctggtgca
      301 ggggggacta cgtcgataca gtgggtaaga acacggcgaa gatacaggat tacataaagc
      361 accagcttga agaggataaa atgggagagc agttatcgat tccctgtccg ggcagcccgt
      421 ttacggaccg taagtaacga agttggatgc aaatgtcaga tcgtgtgcgc ctgttagggc
      481 gcggctggta agagagcctt ataggcgcat ttgaaaaacc tccggctatg ccggaggata
      541 tttattgcac gtcgtcaatt gcggcgtgct ggctcttcca ttgatatctg ctggattaaa
      601 tcgtctggca tatgttcctg taaaatacgc cagaggttct cggcggttct attcagtcgg
      661 gtgttcattc ggtaaatata agcctgaatg gggactgtca atttagcagc atcaaggcat
      721 atcagacgtt tggtctgtag ttcatcgacg atcgaccaga tcggtaacca ggcaatgccg
      781 tatccgttga gcgccatatt ctttaataac tcgctcatcg aagagataaa tatcgtgcgc
      841 ccggtaatac ctccaacctc tgccagatga cgattgacca acctgcccat ataagatgtg
      901 gcggtatagt tgagcaaagg aacctggggc tgagtgatat cgaatacagg gtgcccctgt
      961 gcgtcggcgg cacataccgg gtacaactca gattcaaaca gtttcaggca gcaaaaaggc
     1021 gattgcataa gatcttcatc gcgaaacgaa ataataaagt cactcttgcc ttcgcgtaac
     1081 gtatttacgg cctggacaac atcaatggct tcaacatgat agacaaattc tccgccatag
     1141 gcggtaagtg aatgcaccag tttgggtaat acgctcaggg atagtgagtg cgcggctgca
     1201 attttaatgt tgggaacgcc gagtaaactc tggccgttta actcccccaa attgcattca
     1261 agctgctgta ataagctgcg agtctgggaa tgaaagagct tgccctcttc ggttaattgt
     1321 aaaggggtgg tcgtgcgatc gaataaaaca acgccgatcg ctgcctccag cgccttaatt
     1381 cgtcggctga atgcgggttg cgaaagatta cgctcttttg ccgcctgaga aaaatggcga
     1441 caggcttcca gtgtcaggaa atcgtataac cattttgtct cgatattctt cattaccatg
     1501 gcacctgtat tttcaatcca aagacataat aaaacatccc ggcaataaaa ataatgttct
     1561 caattagcac aacctggcct ttatcattcg ttttgtgcat aaccccttca tttccggtat
     1621 tgacgttgat atcacatttc gccaaaaaaa cgaaattttt ccgtcagata accagaatac
     1681 atcatcacta tggtgaataa gcggaatgtt ccatagctgc atcatgcgtg tcttgcatcg
     1741 ccttgtacaa ccttgcattt ctcatcgccc atttcgactg ttaaaaaaga gagcagcagt
     1801 tatttaccgc gtattgtcat taacgttttg agaggcaggt attgtgaaag gtttaattgg
     1861 tgtactcggt ggaatggggc cggcagctac cgtcgacctg tttaataaat ttgtaacttt
     1921 taccgctgca caatgtgatc aggaacatat tcctttaatt atttcttcga tcccggatat
     1981 tcctgaccgt accgatgcat taatgcatca tgggcgttca ccattacccg ttatgcgtga
     2041 ttatatgcat aagctggaag atgccggggc agaatgtatt gttattccct gtaataccgc
     2101 gcattactgg ttcaaagaat taaaagatgc ttgtcatatt gatatattaa gtatcgtaga
     2161 aactacgatc aatgaagttc gcgcctgtgg taaaacccga attggcttgt tagccaccaa
     2221 tgccacgcta tatatgggtt tataccaaaa aggaattgag tcgctgggat ttacctgtgt
     2281 cagccccgat gccgacggtc agaaaaaagt gatggaaagt atttattcct tgaaagctgg
     2341 caatatagcg cacgcacaaa agttgatgaa tgagcaggct gaaatattat tttcccgcgg
     2401 tgcagaaatc cttgttcttg gttgtacaga aattccggtt attctggcgc aagccgttaa
     2461 agagcagcct tcccgctata ttgactcaac ggcgtcactc gttcgtgccg gaataaaatg
     2521 gtacgaaaat cgtgtcggta aacattatct tttaacccaa taatcattta aatcgcagcc
     2581 agaaagttat tcgcttttaa ctgaattata tttataacgg agaacattat ggtttggctg
     2641 gaaattatcg tagtacttgg tgcaatattt tttggtattc gccagggggg aatcggtatt
     2701 ggtttatgtg gcgggcttgg gcttgccatt ctgactctgg gatttggtct gcctatgggg
     2761 tcgccgccag tcgacgtgat cctgattatt atgaccgttg ttgtggcggc ctcagcctta
     2821 caggccgcag gcggaatgga ttatctggta cgcgtcgctg gtaattttat gcggcgtaac
     2881 ccgaaatata tcaacataat cgcgccgatt atcacctggt ctatgaccat tatggccggt
     2941 actgggttta ttgttttttc cacgctgccg gtgattgctg aagtggcgaa agagtccggt
     3001 atccgtcctt cccgcacgct tgccggttcg gttgtcgctt cgcaggttgc gatttccggt
     3061 tcgccgatca gcgccgcaat ggcggctatg ctcactatta tggaggtaaa cggtgtcagt
     3121 tttattcaag tgatgtcggt atgtcttccc acctcttttg ctgccgcgat ggtcgccgct
     3181 tttattgctt cccgacaggg atgtgagttg caggatgacg aggtctacct tgagcgcctg
     3241 caaaaaggac tggtgcaaaa atacgagaat aatagcagca tcaagccagg tgcgacgctc
     3301 tccgtcggat tgttcctgct ggcgacggtc gctattgtta ttcttgctgc cttcccccaa
     3361 ctgcgtccgg gttttgatat cggtaaaccg atggaaacac gcgatatcat tattatctgt
     3421 atgctctctg ccgcctgcct gatggtggtg ctgtgtaaga cggcgaccga tgccattatt
     3481 ctcacctcca cattccgtgc ggggatgagt tcgctggcgg taattctcgg cattgtaacc
     3541 ttaggcacca cttttattga tgcgcattta actgagatta aagatattgc tggtgatatt
     3601 ttgcaggcgt atccgatgtt gttggcgctg gttctttttg gcacttgcgc gctgctctac
     3661 tcgcaaggtg cgaccacgcc gttaattatc ccgcttgccg tcgcgcttga cgtgcccacc
     3721 tgggcaattc tggcgtccta tgtggccgtc accggagtat ttgtactgcc aacgtatcca
     3781 acatcactgg ctgccatgga atttgatact accggcacaa ctcgtgtagg caaatatgtg
     3841 ctgaatcatc catttatgct gcctgggttg gggggaatta ttgctggagt ggcattaggc
     3901 tttgtgattg cgccaatgat tatttagcac tatcaaacat cagtatcgat ggtttataca
     3961 caataaagac agatatggcg gaatatagtc tgttgtaagc gccaacaaag aataggggaa
     4021 ttccgccatg tctgaacatc ttcacaatat ggtattaagt gttgtcgttt aatgatttat
     4081 ttcttggtta tgttgtgtat tagctgcatc tatcgctttt tgatacctga ggtgtactgg
     4141 caatagcgga cactaccatt tgttcttttt ttaagcagtc atctgatgat atttttccct
     4201 gaaggctgcc g
//
LOCUS       DAZSNO010000239         4197 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZSNO010000239 DAZSNO010000000
VERSION     DAZSNO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.242"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            84..751
                     /gene="ykgH"
                     /locus_tag="ACHWN2_003968"
                     /pseudo
     CDS             84..751
                     /gene="ykgH"
                     /locus_tag="ACHWN2_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
     gene            complement(994..1689)
                     /locus_tag="ACHWN2_003969"
     CDS             complement(994..1689)
                     /locus_tag="ACHWN2_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR8933275.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1682..3109)
                     /locus_tag="ACHWN2_003970"
     CDS             complement(1682..3109)
                     /locus_tag="ACHWN2_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR8933276.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3120..3839)
                     /locus_tag="ACHWN2_003971"
     CDS             complement(3120..3839)
                     /locus_tag="ACHWN2_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR8933277.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1118 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaatggt
      721 agatgaatac caacaaaact ctgggcagta ataaacagca ttctgataga gagtgcctgt
      781 ataaagctga agtgacaatg accgggaagg gaacccgttt tcaggaatgg atgcctgtct
      841 ttttcactgt aaatatcctg gcattactaa tgcctggata tttacagaag agcagcataa
      901 ccatgacgga acggggttaa gtcaggttgt ccttaacgct attttcaacc tcgtatgcct
      961 tcttcaggtt tatgttcaga tctcatatct ctctcagcaa tcctcaataa tcagatacac
     1021 cgctttcacc ggaccatgaa ccccgacgac tttgataagc tcaatatccg ccgttgagct
     1081 gggaccactg atgatgttaa tacatgacgg cattcgttca ccggcctgcg ctttctgatg
     1141 caatttttct gcgagttgcg ctacacgcgg caggattgtg cttttacgca ggataaaaag
     1201 agaagattcc gggagcaggc tcaatgaacg cccgcgctcg gcggcggaaa aaagaaccac
     1261 gcctcccgat tcggttaaac catattcagc atacacgaca cccactttag cctgctctgc
     1321 ctgcgagata ttctcggcac ctttcgccgg atcccaaaca acggcattgc attcctgctg
     1381 caaacgttca ctaatcccca attcctccag cctcgtgtca ccgctaatca cgaccgactg
     1441 atctcccagc tctttacaca gacgtattgc agcttctgcc gccttcgcct cgctggtcag
     1501 ctcacagcgc gtcaacataa catcgctggc aaactgaata aacgcgtcac agcgctgctg
     1561 ttggttaagt tgggtaagcc gctcgttagc atagttgtta agcggcgcat cttctgcttg
     1621 cggttcaagt cgcagcgggc gacccagtgc ctgagcaacg ttattcaaaa attcgctccg
     1681 attatccatt ctttttctcc tgcgcctgat gtttcttaaa ccaactacgg aaactctctc
     1741 cgtcagcttc aggaagatcg cgtgcttcca tccagtcgct aatcgcgcca aatttgagtg
     1801 gtgttttgcc gccattgata aaccagcttg ccgcatgagc gccggccatc atcccgactt
     1861 tccacaatcc tggatgacta ttggcataag cgaacatttt tatcgcccgt tgctctgctt
     1921 ttgcggtgat ccctttttca gccatcaccc gacgatgacg caaaatcagt tttgacagcg
     1981 gaatacgcac cggacacacg ctgtcacaag cagtgcataa agagcaggcg tagggtaaat
     2041 ctttaaaatc tttatagccg ccaagtagcg gagaaatcac cgcaccaatt ggccctggat
     2101 aaatagagcc atatccatga ccgccaatat ggcgatatgc cggacaagta ttcatacaag
     2161 ccccgcagcg aatacagcgc agcacatccc gaaattcaga ggccagcacc tcagaacgcc
     2221 cgttatcgac aataaccaga tgaaactctt caggaccatc aacgtgcccc gcttcgcgcg
     2281 gccctgtcag ccaggtgttg tatcccgtca aacgtgcacc aacggcactg cgcgccagca
     2341 tggtgatcaa tacatctacc tcggcaaacg tgggggcaat acgctccatt cccatcactg
     2401 caatatgcgt tttaggcagc gtggtacaca ttcgcgcatt accttcattg gtcaccaggc
     2461 ataccgaacc ggtctctgcc accgcgaaat tacagccggt aatacctatt tcagcactga
     2521 ggaaatcttc acggattttt tgccggatga ataaggtcat cgcttcaggc gtttccggcc
     2581 cctcatagcc cagacgttcg tgtagcactc gacggatctg atggcgatct ttatgaattg
     2641 ccgggaccac aacatgagat ggcggatctt gatccagctg gagaatatat tcacccagat
     2701 cggtttcaat cacctgaatg ccagcatcct gcaacacatg attgacacca atctcttcgg
     2761 tcaccatcga tttagatttc accaccttcc gggcattttt gcgttgggca acctgtaaaa
     2821 tgtagcgggt agcgtcttct ttggtttttg caaaatagac gtgaccgccg ttttgcgtca
     2881 ctttttctga gagctggtac agataagcgt cgagattact cagaacatga tcacgtatct
     2941 gggcggcccg atcgcgccac tcctcccagt gccccaattc atcgaccatt ttttgccgat
     3001 ttgccccaat acgctgctgc gcgcttgcca ccgctttgcg catgatcgga tcttcaattt
     3061 gctgacggat gcgtgtctta aaatctgtat tactggtttt gatcgacatc tttatatcct
     3121 cagcggctca tcaacacttc agcaatatgc atcactttga ctttctgccc ttcccgttgt
     3181 aatcgcccac tgatgttcag caggcaactc acgtcagcac caattaaata ctcagggcgg
     3241 acttccatca ggtgcacaac cttttctttc accatctcgc cggatatttc ggccattttg
     3301 accgagaacg tgccgccaaa tccgcagcag gtatcctgtt cagcaaaggt caacagctcc
     3361 agtccacgca catttttcag cagcgtaagt ggctcgtcct tcactcccag cttacgggcc
     3421 aggctacaag atgggtgata cacagctctc ccttgcaaac tggcacctac atcgactacc
     3481 cctaatttat taacaataaa agaggtgaga tcctgcatac gcgcggcaac cttttcggca
     3541 cgtgatgccc attcaggttc atccgccaga tacgtcgggt aactttttat ggcataggtg
     3601 caagagccag ccggtgaaat aatgggatcg tcgttatcct ccagtgcggc gatcagattt
     3661 ttcatccctg gaatcgcttc tttgatataa ccgctattga tcgcaggctg accgcagcat
     3721 ccctgtttct ccgggaaatt tacgcgacag ccgagttttt ccagtagcag cacggagtct
     3781 cgtgccattc ttgatttcag ggcgtcacca atacaggtga caaagaaatt gacattcaca
     3841 actaatactc cattacttca tgcccattta tgcccgatgt ttccccccca ccgggggatc
     3901 aatcatcaat aaatatggcg ttctttttta attggctagc aacataccac ccctcatttg
     3961 tatgacaaat gataatcttt catccaaact tcaaatgcaa taactaattt tatttctttc
     4021 ggcatcgctc acattttatg gggaaatcga cgatcatttt tggcataaga aatccattta
     4081 aaaatggcta catcccttat acctattcag agctacttat tcaggggtta ttgagatata
     4141 cactgatggt gtacaaaaaa atcaccgaca ataatcacgt atcaagtaat agtcgga
//
LOCUS       DAZSNO010000240         4185 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZSNO010000240 DAZSNO010000000
VERSION     DAZSNO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.243"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..192
                     /locus_tag="ACHWN2_003972"
     CDS             <1..192
                     /locus_tag="ACHWN2_003972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933278.1"
                     /translation="SIQSNRDWLSMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGIT
                     NIISIILQQAQDYDVAKIT"
     gene            complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACHWN2_003973"
     CDS             complement(350..997)
                     /gene="ychE"
                     /locus_tag="ACHWN2_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR8933279.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACHWN2_003974"
     CDS             complement(1028..1081)
                     /gene="ychE"
                     /locus_tag="ACHWN2_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFR8933280.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1474..4149
                     /gene="adhE"
                     /locus_tag="ACHWN2_003975"
                     /gene_synonym="adhC"
     CDS             1474..4149
                     /gene="adhE"
                     /locus_tag="ACHWN2_003975"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR8933281.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1169 a         1021 c          959 g         1036 t
ORIGIN      
        1 tcaattcaat caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt
       61 agtgaaatgc acaataattt aacaattgat tattatttag cactttcctc taccaaaggc
      121 agtggaatta ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg
      181 aaaataacat gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg
      241 agaagaatat ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa
      301 taatgacgaa gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca
      361 ggccggggaa aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca
      421 atagccccat aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc
      481 acggtgccat gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga
      541 aaccaaacag atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg
      601 cccctggccc cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg
      661 cggtttctga tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg
      721 acatcgctat cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac
      781 caaaaagttg tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag
      841 acaggttggc tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc
      901 tgataaagac gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt
      961 tgaagtaaac gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg
     1021 gataatgtta gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca
     1081 tttttcacct cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa
     1141 aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg
     1201 aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta
     1261 cccagaagtg agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc
     1321 taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt
     1381 tcattggtta agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt
     1441 actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt
     1501 aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag
     1561 caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc
     1621 gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac
     1681 cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg
     1741 tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt
     1801 atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag
     1861 acccgtaacg ccattatctt ctccccgcac ccacgtgcaa aagatgcaac caacaaagcg
     1921 gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg
     1981 atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg
     2041 atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct
     2101 atcggtgtag gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt
     2161 gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa
     2221 cagtctgttg ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac
     2281 ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat
     2341 ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc
     2401 ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc
     2461 gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc
     2521 gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct
     2581 tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg
     2641 aaaacagctc gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat
     2701 aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc
     2761 tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa
     2821 aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc
     2881 gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc
     2941 ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa
     3001 actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca
     3061 gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg
     3121 gacgccgcga agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg
     3181 gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa
     3241 gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg
     3301 gttgtaactg acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg
     3361 gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc
     3421 ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag
     3481 ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc
     3541 taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc
     3601 gcgggtatcg catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg
     3661 ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt
     3721 cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg
     3781 caggctcgcc gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac
     3841 cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg
     3901 ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg
     3961 gataaactgt ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg
     4021 ctgatctccg agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa
     4081 gatgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa
     4141 tccgcttaat cagtagcgct gtctggcaat ataaacggcc ccttc
//
LOCUS       DAZSNO010000241         4182 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZSNO010000241 DAZSNO010000000
VERSION     DAZSNO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.244"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..186)
                     /locus_tag="ACHWN2_003976"
     CDS             complement(<1..186)
                     /locus_tag="ACHWN2_003976"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR8933282.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHWN2_003977"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHWN2_003977"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR8933283.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="ACHWN2_003978"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="ACHWN2_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR8933284.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="ACHWN2_003979"
     CDS             3863..4144
                     /locus_tag="ACHWN2_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR8933285.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1044 c         1062 g         1060 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagc
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAZSNO010000242         4168 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZSNO010000242 DAZSNO010000000
VERSION     DAZSNO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.245"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHWN2_003980"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHWN2_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR8933286.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHWN2_003981"
                     /pseudo
     CDS             complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACHWN2_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHWN2_003982"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACHWN2_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            3787..>4168
                     /locus_tag="ACHWN2_003983"
     CDS             3787..>4168
                     /locus_tag="ACHWN2_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFR8933287.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1021 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAZSNO010000243         4087 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZSNO010000243 DAZSNO010000000
VERSION     DAZSNO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.246"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1024)
                     /gene="bglB"
                     /locus_tag="ACHWN2_003984"
     CDS             complement(<1..1024)
                     /gene="bglB"
                     /locus_tag="ACHWN2_003984"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR8933288.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1043..2920)
                     /gene="bglF"
                     /locus_tag="ACHWN2_003985"
     CDS             complement(1043..2920)
                     /gene="bglF"
                     /locus_tag="ACHWN2_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR8933289.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3054..3875)
                     /gene="bglG"
                     /locus_tag="ACHWN2_003986"
     CDS             complement(3054..3875)
                     /gene="bglG"
                     /locus_tag="ACHWN2_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR8933290.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
BASE COUNT         1091 a         1066 c          893 g         1037 t
ORIGIN      
        1 atccaaccgg atcaatttgc cacccccact ctgaactttt cagatgcgga ttggggatca
       61 tattcagtat gttgccctgc gcatttttat taatgctttc gtcgtgggaa acacaaccag
      121 tcatgtaata actaaaagag atgaaatcta cagtatgttt taaatcttct gcgtcacttt
      181 cagtcatctc aatggtgata ttgtggtcgc ggaagaaacg ctgcatatag ccgggatatt
      241 ggccacgcgc ctgaacatca ccaaagaaca tccagcgccg gttctcttcc atggcctgca
      301 acatatcctg tggctggcag gtgagggggt aaaccagccc accgagaagc atattgccga
      361 ttttcgcttc ggggatcagg ctatgatagg ctttaactgc ccgcgcactg gcaaccagtt
      421 gatgatggat cgcctgataa acttccgcct cgccactctc ttctgccagc cccacgcccg
      481 tgaatggcgc gtgtaacgac atattgattt cattaaacgt cagccataac gctactttat
      541 gttggtagcg ggtaaagacc gtgcgggcgt aatgctcgaa gtgatcgatg accgcccgat
      601 tagcccaacc gccgtagttt ttcaccagcc catatagcat ttcgtaatgg gataacgtta
      661 ccagcggctt gatccccgcc tgcgccattt catcaaacag ccgatcgtaa aacgctaacc
      721 ccgcttcatt cggttcggct tcgtcgccct gagggaaaat tcgcgcccag gcaatggaaa
      781 tacgcagaca ggtgaagccc atctcggcaa ataacgcgat atcttccggg taacggtaat
      841 aaaaatcgat ggcgacatct ttgatattct ctttccccag gatgcgcggt tccatttttc
      901 ccattacgcc atgaggctgt aaatctgagg tcgtgatccc tttgccatct tccagccagg
      961 caccttccac ctgattggca gctgttgcgc caccccaaag aaatgtttct ggaaatgctt
     1021 tcataattaa ctccttttat cgttagcgaa tgatggataa cagcggttca cctgcgctta
     1081 tctgcgccgt gccgtggggt aatacgtctg taaaatcatc gctattactg attaataccg
     1141 gcgtcgtcag atcaaatccg gcctcgcgaa tagcagggat atcaaaagaa atcagccgat
     1201 cgcctgtatt gaccttgtca cccacgttga cgtgagcgga aaagaatttg ccgtccagtt
     1261 ttacggtgtc gataccgaca tgaatcagga tctccacacc atcatctgac tcaatgccaa
     1321 tggcgtgtaa tgtggcgaac aacgaagcaa ttcgacccgc aaccggagaa cgcacttcac
     1381 caaccgaggg cagaatggcg atacctttac ccaacaggcc actggcaaac gtggtatcag
     1441 cgacgtgaat gagcggcaca atctctcccg tcatcggtga acagataccg ccctgctcag
     1501 gtggtgtaat aacctctggt gttttctctt gcggggcacc ctgcgctggc tgacgtttac
     1561 tggtgatgaa atgaagcatc aacgtaccaa caaatgcgca acctatggca atgacaccgc
     1621 caataacgct ggcccagacg gtgaaatcaa tccccgttga cgggatggtc tgcatgaagg
     1681 tgaaaatact tggcaaacca aaggagtaaa ctttcgtttg cgcgtagcca atgatggtgg
     1741 cccccaatgc tccactgata caggcgataa caaaggggta cttacgcggc aggttgacgc
     1801 catataccgc tggttcggtg ataccaaaca gacccgtcaa cgccgctgat cccgccacca
     1861 cttttttctg cgcatcgcgt tcgcagagga agacgccgag cgccgccccg acctgcgcca
     1921 taatggcggg cattaacagc gggatcatgg tgtcgtagcc cagcacggtg aagttattga
     1981 tacacagcgg caccaggccc ctgtgcagtc cgaacatgac gaagatttgc cagaagccgc
     2041 ccattaccgc gcccgcaaat gcaggaaccg cctgataaag ccagagataa ccggcggcaa
     2101 tcatttcgct tatccaggtt gatagctgcc ccaccagcag aaaggtgacg ggtgtgataa
     2161 ccatcagaca tagcaatggt gtgaagaaat ttttgattgc cgacggtaac cacgcattaa
     2221 gtcggcgttc cagaatgctg cacaaccagg cagaaaaaat aatgggaata accgatgacg
     2281 agtaattcaa caatgtgacc ggaataccca ggaaatccag ccccggcgca tccgcttttt
     2341 gcccgttctc gaaggcagtc agaattaatg gatgcactaa cgctccacca atcaccatgg
     2401 cagtaaatgg attaccgccg aagcgtttgc ccgcggtgta tcccaggatt atcgggaaga
     2461 acccaaacaa ggcatcactg gcgctgaata aaattaaata agtaccactt tgttcggtcg
     2521 tccactgaaa agtgagcgcc agagccagca tacctttcag gatcccggtt gccgccatca
     2581 gaccgatcag aggcgtaaaa atacctgaaa taacataaac aaagcggttt agcagattac
     2641 ctttctcatc attttccggt gcctgttgcg ctttttcgcc aaggcctgcc acgctgttaa
     2701 ccgccaggaa gacatcggcc acatggttac ctatgaccac ctgaaactga ccaccgcttt
     2761 ccaccaccat aataataccg ggggtctttt tcagtacctc agcttgcgct ttgctttcat
     2821 cctttaactt aaaacgtaat cgcgttgcgc aatgcatcag actcacaatg ttatctgcgc
     2881 ccccgactcc tgcgactatt tttctggcta actccgtcat aacttgccct ctaccgcttt
     2941 gcggcaaaac tccaaaaaaa aacctgaaaa aaacggcctg acgtgaatca agcaattttt
     3001 ttcaggtttt gcccgcttag tgcggtaaca atcctttact cagtaataat atttcagtgt
     3061 tctttgcgca cgcgctctat atttatggct aaaaacataa tctctgcggg tgaaatttta
     3121 cgttgatact gcaaaccaat aaaaatggcg atccgttccg cacattgcca tgcttgcggg
     3181 taattttgtt ttactgcttg ttgtaatgat tcatcactat cgttaattga agcatgttca
     3241 agaatacgcc aggataaaaa cttcagatgt gtaaccagtc gctgataact caagctttct
     3301 tcctggtaat taaggctgaa ctgaaatttt attaattgca gcatttcgcg cattaactgc
     3361 gtgacacctg caacatcctc catatttccg ctcatttggg cactgaccag atgcatggca
     3421 ataaagccca cttcatcttt cggtaactgc acgcccaacc gtttatcaat aatggttaac
     3481 gcttcttccc ctagctggaa ctctttcggg taaagccgct ggatatccca cagcaacacg
     3541 ttttgctgaa agcgtttaat cgcaaactgg caatggtcag ttagcgagat ataaatactg
     3601 tcctgtaatt ttcccaagcg ctcctgcgct aaagagataa gacgatcaca ggttgccatc
     3661 acctcaagag gaatatgact taagagttcg cttaatcgcc cgttcagttc atgactgctc
     3721 aaggcatact ctttttctat tccacttgag ttaattcttt cgccagcgcg tttttgaaag
     3781 ccaattccgc gccccatgac gactttttcc cgctgttgat catcaataac caccacaaca
     3841 ttattgttga gaattttggt gatttgcatg ttcatagcaa ggaccttttt tataaacaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
     4081 aactttg
//
LOCUS       DAZSNO010000244         4083 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZSNO010000244 DAZSNO010000000
VERSION     DAZSNO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.247"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            353..1711
                     /locus_tag="ACHWN2_003987"
     CDS             353..1711
                     /locus_tag="ACHWN2_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFR8933291.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(1833..1910)
                     /locus_tag="ACHWN2_003988"
                     /pseudo
     CDS             complement(1833..1910)
                     /locus_tag="ACHWN2_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
     gene            complement(1950..3335)
                     /locus_tag="ACHWN2_003989"
     CDS             complement(1950..3335)
                     /locus_tag="ACHWN2_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR8933292.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="ACHWN2_003990"
     CDS             complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="ACHWN2_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8933293.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3729..>4083)
                     /locus_tag="ACHWN2_003991"
     CDS             complement(3729..>4083)
                     /locus_tag="ACHWN2_003991"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8933294.1"
                     /translation="RKGCPNYPPEFKQLLVAASCEPGISISKLALENGINANLLFKWR
                     QQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFRHGTLRFNGN
                     VSEKLLTLLIQELKR"
BASE COUNT          944 a         1102 c         1130 g          907 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtca ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttata gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggcgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcgcg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac ccccaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgcgccacca gtggaaaaag gccataagca gtccgaacaa aagtaagcgt
     1921 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     1981 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     2041 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgct
     2101 ccccgctcac caccatggcc agagccgaag aacagggtct tcatcttttc acgcaaccag
     2161 ctttccaggg atttcaacag cggtttcgtt tttcgctgac gttcagcaag ccgctgctct
     2221 gccggcattc cccttatatc cgcctctatg gcgtacaact gaccgatctg ctccagggct
     2281 tcttccgtca gtgctgacgg gatgcggacg tgcacatcgt ggatctttcg gcgggcatga
     2341 gcccagcagg cagcttccgt tatcccacca ttgcgataca gctcgttgaa cccggcgtac
     2401 gcatccgctt gcagcacacc gctgaagcaa gcaagatgag tctgcggatg gatgcttttt
     2461 ctgtccgggc tgtaagcgaa ccacactgca ggtgccaacg ctgacccggc attgcggtca
     2521 tcacgaacat acgcccacaa ccgcccggtc ttcgtcttct tattacccgg cagcagtacc
     2581 tggaccgggg tatcatcggc atggagtttg ccgtcagtca tgacatagcc atgaagcgcc
     2641 tcttccagcg gagacagcag ccggcagcat gcatccaccc agcccgacag cagtgaacgg
     2701 ctcagctcca caccttgccg gccgtatatt tctgactggc aatacagcgg ggtgtgctct
     2761 gcatacttcg aggtcagcac gcgggccagc agccccggtc cggcgatacc ccgctcgatg
     2821 ggccgcgaag gtgcaggtgc ctgcacgatg gtatcgcact gagtacaggc atgtttttcc
     2881 cgtaccgtcc ggataacccg gaaggcgcta cgcatcaact ccagctgttc ggcggtatcc
     2941 tcgcccagat agctcagtga accgccgcag ttcgggcagc acggcgccgc aggcaacagt
     3001 cgcttttcgt cacggggtag tgattcaggg aacggcttac gggtgcgggt ctgacgcaac
     3061 ggacgctgta ctgccgggtc atacacccta ccagtcagcg tatcgctctc tttctgaagc
     3121 cggttcagat cggcttccat ttgtgcgata cggcgggaga ctttttcgga acgactgccg
     3181 aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc gcgctcccgg
     3241 ttgctcagct tttcctgcag ggcgtggatc agcgcttcct gttcggccag gcgctgtttc
     3301 agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc cgtattttac cgggcttatt
     3361 ctgtgacaac caggataaag agatttacag catggtcagg gaggtcagca gccgcttggg
     3421 ctgtcgccag tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt
     3481 gccatcacgg gctgacggcc aggcgaagcg cccacgctcc agccgtttgg tcaggaggca
     3541 cagtccgtcg ccggtggacc acagcagttt aacctgactg ccgctgcggc cccggaaaat
     3601 gaaaatatgg ccggacatgg gatcgtcttt cagcgccgtt tgtactttcg cagccaggcc
     3661 gttgaagcta tttctcatat cggtgatacc ggcaaccagc caaattttgg tcccggaagg
     3721 taacgggatc atcgcttcag ttcctgtatc agcagagtca ggagcttttc gctgacattg
     3781 ccattgaagc ggagcgtccc gtgccggaac gttacctcac agctgatact gagggtttcc
     3841 gggtcctctg cgagcgattc tggctgttcg gcagctgcat cgagagtcac aggaagtagc
     3901 tgggggctct ctgaagaagg taatagcagc tttccctcgc gccattgttg tcgccatttg
     3961 aacaacagat tggcgttaat gccattttca agagcaagtt ttgagatgga tatcccgggt
     4021 tcacaggagg cagcaacgag cagctgttta aattcgggag gataattagg gcagcctttt
     4081 cgc
//
LOCUS       DAZSNO010000245         4042 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZSNO010000245 DAZSNO010000000
VERSION     DAZSNO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.248"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(126..284)
                     /locus_tag="ACHWN2_003992"
     CDS             complement(126..284)
                     /locus_tag="ACHWN2_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR8933295.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
     gene            complement(886..1077)
                     /locus_tag="ACHWN2_003993"
     CDS             complement(886..1077)
                     /locus_tag="ACHWN2_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR8933296.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENR"
     gene            1142..1465
                     /locus_tag="ACHWN2_003994"
     CDS             1142..1465
                     /locus_tag="ACHWN2_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR8933297.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1674..2105
                     /locus_tag="ACHWN2_003995"
                     /pseudo
     CDS             1674..2105
                     /locus_tag="ACHWN2_003995"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="ACHWN2_003996"
     CDS             2143..2673
                     /locus_tag="ACHWN2_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR8933298.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="ACHWN2_003997"
     CDS             2680..2913
                     /locus_tag="ACHWN2_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR8933299.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="ACHWN2_003998"
     CDS             complement(3459..3851)
                     /locus_tag="ACHWN2_003998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933300.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          965 a          918 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcagc ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAZSNO010000246         3888 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZSNO010000246 DAZSNO010000000
VERSION     DAZSNO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.249"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            296..742
                     /locus_tag="ACHWN2_003999"
     CDS             296..742
                     /locus_tag="ACHWN2_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR8933301.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            753..2204
                     /locus_tag="ACHWN2_004000"
     CDS             753..2204
                     /locus_tag="ACHWN2_004000"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR8933302.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2194..3264
                     /locus_tag="ACHWN2_004001"
     CDS             2194..3264
                     /locus_tag="ACHWN2_004001"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR8933303.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            3264..>3888
                     /locus_tag="ACHWN2_004002"
     CDS             3264..>3888
                     /locus_tag="ACHWN2_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFR8933304.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT          892 a         1001 c         1032 g          963 t
ORIGIN      
        1 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
       61 attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat
      121 gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
      181 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
      241 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
      301 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
      361 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
      421 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgcagt
      481 gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc gcaaagcgca
      541 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
      601 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
      661 tcaccaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc tggccttgtt
      721 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
      781 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
      841 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
      901 accgcttatc cagcgaagag attgccgctg ccgattacgt cattcttgct accgggcgtg
      961 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
     1021 ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
     1081 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
     1141 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
     1201 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
     1261 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
     1321 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
     1381 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
     1441 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
     1501 tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
     1561 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
     1621 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
     1681 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
     1741 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
     1801 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
     1861 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
     1921 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
     1981 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
     2041 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg
     2101 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
     2161 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
     2221 tgcgaagcca gcgccgttag cggcgatgaa caggaagttc gcgacatcct gataaacacg
     2281 ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt tgcccgtaag
     2341 ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
     2401 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
     2461 tcgatgctca accatcgggt aaccatacgc acacacaagg gagtgaaaat ccctggtgtg
     2521 attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt
     2581 gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg
     2641 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
     2701 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
     2761 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
     2821 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt gctggatacc
     2881 gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
     2941 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
     3001 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccgga
     3061 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
     3121 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
     3181 ctgctcacgt tgatacgggg ttttctgacg accttaaccg cggagaaagt caacgcgttt
     3241 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
     3301 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
     3361 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
     3421 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
     3481 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat actttcactc
     3541 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc
     3601 cccagttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct ggtttgggcc
     3661 atttcattga tgagacagaa gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag
     3721 atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat ttatccacgg
     3781 cctgcgacaa ccaacaccgc tggccgctca tacagggtga ttatctctcc agcctgattc
     3841 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaat
//
LOCUS       DAZSNO010000247         3873 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZSNO010000247 DAZSNO010000000
VERSION     DAZSNO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.250"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHWN2_004003"
     CDS             complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHWN2_004003"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR8933305.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1572..1768)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2005..3654
                     /gene="pgi"
                     /locus_tag="ACHWN2_004004"
     CDS             2005..3654
                     /gene="pgi"
                     /locus_tag="ACHWN2_004004"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR8933306.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT          982 a         1032 c          975 g          884 t
ORIGIN      
        1 ttttctgctc attattagct ccttctgatg ccattctatt tcaggaagga gtgtccgtta
       61 aactcaggct acctcatagt gacaagaaaa tcaatacggc ccgaaatata gcttccaggc
      121 catacagtat ttactcaaac aaattactat gcagtttttg caccacctgc tcggcatctt
      181 cgccgggcac caggaagcac aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt
      241 tgaacggttc cagtacgccg aatacctctt tgccaacgcc gcaggctttt gacaggtcat
      301 tgccaatcaa cgcgaccagc gccagacctt cttccacctc cacccggcac agtgcggaaa
      361 gctccatcag cagagattgc gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat
      421 caagggttaa tgccacgctc acttctgacg tggtgattaa gtctaccgaa atattatgcc
      481 gcgcgaggat gccgaaaact tccgcgagga agccgcgaga atgcagcata ttcaggctgt
      541 gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt
      601 cagttttatt gcacaccagc gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga
      661 ccgggatatc gctgcgtact gcgggtagca acgttgccgg atgcagtact tttgcaccaa
      721 aagttgccat ctctgccgct tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa
      781 ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt ccagatatca acacgagatg
      841 cgtgtaaagc ctccgccagc aaggctgccg tataatcgct gcctccacgg ccaagcgtcg
      901 ttgtacgacc tttattttcg ctaccgataa atccctgggt gatcactaag ccttcattga
      961 ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc ggctacatct ggctctgcac
     1021 gaccaaatcg gtcgttggta cgcatcactt tacgtacatc aaaccactgt gcctgaacat
     1081 cgcgttcgcg caggatttcg acaaacagca gggtcgacat cagctcgccg tggctgacca
     1141 gctcatctgt cagcgccgga gacgttgcca gcgccgccgc ttctgccaga acagtaatgt
     1201 tctccagcag acgttcaatc tcttcacgga taacgttcgg gtaacgcaga cgttccagaa
     1261 tggcaaactg gatgttgcga atagcgtcga gtttttcgaa tcgctcgcca ggttccagcc
     1321 cttccgctaa agcgaccagc agattagtga taccagcaga agccgagagg actactaaac
     1381 gcacgttggc atcagaaagc acaatatcag cgctgcggtt catggcgtca aaatcagcta
     1441 cgctggtacc gccaaatttg gagacaacaa tttcagacat aactacctcg tgtcagggga
     1501 tccattttca gccttggcac aagggaagag cggaagacgg gtgggcgcag agcgatactt
     1561 cgctactatt ttcacccaga agtgctccac cacttgcgaa acgcccgact gcgaacgctt
     1621 ctggtgacaa cccaggggat tcagcccctg tagccgatga tgaacgtggc cagccgttca
     1681 atcacctcgg cgatgcaccc cctcaggtgt tatcacagga ctggctcctc caacaccgtt
     1741 acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg tagtacattt ataaataaag
     1801 ggtgagcggg gcggttgtca acgacggggt catgcggatt tttcatccac tcctggcggt
     1861 cagtagttca gctaataaat gcttcactgc gctaagggtt tacactcaac attacactaa
     1921 cggcactaaa accatcacat ttttctgtga ctggcgctac aatcttccaa agtcacaatt
     1981 ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc caacgcagac cgctgcctgg
     2041 caggcactac agaaacactt cgatgaaatg aaagacgtta cgatcgccga tctttttgct
     2101 aaagacggcg atcgtttttc taagttctcc gcaaccttcg acgatcagat gctggtggat
     2161 tactccaaaa accgcatcac tgaagagacg ctggcgaaat tacaggatct ggcgaaagag
     2221 tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg agaagatcaa ccgcactgaa
     2281 aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca ataccccgat tctggttgat
     2341 ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga agatgaaaac cttctcggaa
     2401 gcgattatct ccggtgagtg gaagggttat accggcaaag caatcactga cgtagtgaac
     2461 atcgggatcg gcggttctga cctcggcccg tacatggtga ccgaagcact gcgtccgtac
     2521 aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg ggactcacat ctcggaagtg
     2581 ctgaaaaaag taaacccgga aaccacgctg ttcctggtag catctaaaac cttcaccact
     2641 caggaaacca tgaccaacgc ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat
     2701 gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca atgccaaagc cgttggcgag
     2761 tttggtattg atactgccaa catgttcgag ttctgggact gggtcggcgg ccgttactct
     2821 ttgtggtcag cgattggcct gtcgattgtt ctctccatcg gctttgataa cttcgttgaa
     2881 ctgctttctg gcgcacacgc aatggacaag catttctcca ccacgcctgc cgagaaaaac
     2941 ctgcctgtcc tgctggcgct gattggcatc tggtacaaca atttctttgg tgcggaaact
     3001 gaagcgattc tgccgtatga ccagtatatg caccgtttcg cggcgtactt ccagcagggc
     3061 aatatggagt ccaacggtaa gtatgttgac cgtaacggta acgttgtgga ttaccagact
     3121 ggcccgatta tctggggtga accaggcact aacggtcagc acgcgttcta ccagctgatc
     3181 caccagggaa ccaaaatggt accgtgcgat ttcatcgccc cggctatcac ccataacccg
     3241 ctctctgatc accaccagaa actgctgtct aacttcttcg cccagaccga agcgctggcg
     3301 tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg atcagggtaa agatccggca
     3361 acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta accgcccgac caactccatc
     3421 ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa
     3481 atctttactc agggcgtgat cctgaacatc ttcaccttcg accagtgggg cgtggagctg
     3541 ggtaaacaac tggcaaaccg tattctgcca gagctgaaag atgataaaga aatcagcagc
     3601 cacgatagct cgaccaatgg tctgattaac cgctataaag cgtggcgcgg ttaatcatca
     3661 tgcgtgcctg atgccgatat gtaggccgga taaggcgttt acgccgcatc cggcaaccgg
     3721 agcctgatgc caatatcgac attaatgcat agtatgagga gttaaataac cttaaaaaca
     3781 atgccgacaa taagtcggca ttgttttatc agataaatcc ccttgtctgt gatttaacgg
     3841 aaatcatacc gtgaggttaa tcctaaaata gat
//
LOCUS       DAZSNO010000248         3872 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZSNO010000248 DAZSNO010000000
VERSION     DAZSNO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.251"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            112..1446
                     /gene="adeQ"
                     /locus_tag="ACHWN2_004005"
                     /gene_synonym="yicO"
     CDS             112..1446
                     /gene="adeQ"
                     /locus_tag="ACHWN2_004005"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR8933307.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1499..1951
                     /locus_tag="ACHWN2_004006"
     CDS             1499..1951
                     /locus_tag="ACHWN2_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR8933308.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2162..3352
                     /gene="nepI"
                     /locus_tag="ACHWN2_004007"
     CDS             2162..3352
                     /gene="nepI"
                     /locus_tag="ACHWN2_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFR8933309.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3393..3686)
                     /gene="yicS"
                     /locus_tag="ACHWN2_004008"
     CDS             complement(3393..3686)
                     /gene="yicS"
                     /locus_tag="ACHWN2_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR8933310.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
BASE COUNT          798 a          862 c         1022 g         1190 t
ORIGIN      
        1 attaaagaat atctattaat gtgcaattga aatgtgataa ttatcactga accgttgttg
       61 tcatttgttt tacaaaagca agggattaaa tctcctccaa tggacaaaaa aatgaataat
      121 gacaataccg attacgtgag taatgaatca gggacgcttt cgcgattatt taaactacct
      181 cagcatggga ccaccgtccg cacagaattg attgcgggga tgaccacttt tttaaccatg
      241 gtgtacattg tttttgtgaa cccgcaaatc ctcggcgcgg cacaaatgga cccgaaagtg
      301 gtgtttgtta ccacctgttt gattgccggt atcggcagta ttgcgatggg gatatttgct
      361 aacttacccg tggcgctggc tccggcaatg gggctgaacg ccttctttgc gttcgtggtc
      421 gtgggggcga tggggatctc ctggcagacc gggatgggcg cgatattctg gggcgcaatt
      481 gggctatttt tgctcacgct gtttcgtatc cggtactgga tgatctccaa cattccatta
      541 agtttacgta ttggtatcac cagtggaatc ggattattta ttgccttaat gggattaaaa
      601 aacactggtg ttattgtcgc caataaagac acgctggtga tgattggcga tttaagttct
      661 cacggcgtgt tgttaggtat tttagggttt tttattataa ccgtgttgtc atcacgtcat
      721 tttcatgccg cggtgctggt ttctattgtg gtgacgtctt gctgtggatt atttttcggt
      781 gatgttcatt ttagcggcgt ctattccatt ccgcctgata ttagcggcgt cattggtgaa
      841 gtagatttga gcggcgcgtt aacacttgaa ctcgccggta tcattttctc ctttatgctg
      901 atcaacctat ttgattcatc aggaacatta attggtgtaa ctgataaagc gggcttaata
      961 gatagcaacg gtaaattccc caatatgaat aaggcgctgt atgttgatag cgtcagttcg
     1021 gtggcaggcg catttatcgg tacctcgtct gttactgcct atattgaaag tacttctggt
     1081 gtggcagtcg gtggtcgcac ggggctaacg gctgtcgtgg tcggcgttat gttcctgtta
     1141 gttatgttct tctcaccgct ggtggcgatg gttcctcctt acgcaaccgc tggagcgtta
     1201 atctttgttg gcgtgctgat gacttcgagc ctggcgcgcg ttaactggga tgattttacc
     1261 gaatcggtgc ctgcgtttat taccacggtg atgatgccct ttactttctc gatcaccgaa
     1321 gggattgcac tcggctttat gtcgtactgc atcatgaaag tgtgcaccgg gcgctggcgc
     1381 gatctgaacc tgtgtgtggt ggtggtcgca gctctgttcg cattgaagat tattctggtg
     1441 gattagtaac tggcgagggt ttccagatat catgagttct gattacgcag gagaactcat
     1501 gatctggata atgctcgcca cgctggcggt agtgtttgtg gttggttttc gggtgctgac
     1561 atccggggcc agaaaagcga ttcgccgtct cagcgatcgg ctgaatatcg atgtcgtacc
     1621 cgtggagtcg atggtcgatc aaatgggaaa gtcagccggt gacgaatttt tacgttattt
     1681 gcatcgcccg gatgagtcgc acctgcaaaa cgccgcgcag gtgttgctta tctggcaaat
     1741 tgtcattgtc gatgatagcg aacagaacct gctgcaatgg caccggattt tacaaaaagc
     1801 ccgcctttcc gcgccgatta ccgatgctca ggtcaggctg gcgctgggtt ttctgcgtga
     1861 aaccgaacct gaaatgcagg atattaatgc ttttcagatg cgctataacg cgttctttca
     1921 gcctgccgag ggcgttcact ggttgcattg aaaccgtttc gtcttatcag cctacagacc
     1981 gcgcctgacc tgtcgggtgg ataagacgtt cacgccgcat ccggtaatct ttgccctgtt
     2041 ccgtttcgat cttaactcca gctcaagcaa caccgtaaaa gttgcaaaaa cgttaaattc
     2101 gtcacacatt tcaatgttaa agtggtcggc ttttcccctg aaacatgcca cgggtaacac
     2161 catgagtgaa tttattgccg aaaaccgcgg cgcggatgcc atcacccgac cgaactggtc
     2221 agccgttttc tcggtggcgt tttgtgtcgc ctgtctgatt atcgttgagt ttttgcccgt
     2281 cagtttgttg acgccaatgg cccaggattt aggcatttcg gaaggggttg ccgggcaatc
     2341 ggtgaccgtg accgcctttg tggcaatgtt tgccagtttg tttattaccc agacaattca
     2401 ggccactgac cgccgctacg ttgttatttt gtttgccgtt ttgctgacgc tctcctgctt
     2461 gctggtttcc tttgctaact cattcagttt gcttttaatc ggtcgtgcct gtctggggct
     2521 ggcgctgggc gggttctggg cgatgtcggc gtcgctgacc atgcgtctgg taccgccgcg
     2581 tacggtgccg aaggcgctgt cggtgatctt cggcgcggtt tctattgcgc tggtgattgc
     2641 cgcgccgttg ggcagttttt taggcgagct tatcggttgg cgcaatgtct ttaatgcggc
     2701 ggcggtgatg ggcgtgctgt gtattttctg gattatcaaa tcattgcctt cactgccagg
     2761 cgaaccctcg catcagaaac aaaatacttt ccgcttatta caacgtccgg gtgtgatggc
     2821 agggatgatc gccatcttca tgtctttcgc cgggcagttt gctttcttca cgtatattcg
     2881 cccggtgtat atgaacctgg cgggattcgg cgtggatggc ttaacgctgg tgctgttgag
     2941 ttttggtatc gccagcttta ttggtacgtc gctttcgtcg ttcattctta aacgttcggt
     3001 aaaactggcc ttagcaggcg cgccgttaat actggctgtg agtgcgttgg tactgacgtt
     3061 gtggggaagc gataaaatcg ttgctaccgg cgtggcgatt atctgggggc taacttttgc
     3121 attggttccc gtcggctggt caacgtggat cacccgctcg ctggccgatc aggcagaaaa
     3181 agccgggtct attcaggtgg cggttattca gcttgctaat acctgtggcg cggcaatcgg
     3241 cggttatgcg ctggataata ttggtctgac ttcgccgttg atgttgtccg gcacattgat
     3301 gttgctgact gcattgttgg ttactgcaaa ggtgaaaatg aagaaatcct gatatcaggt
     3361 aaagaagcgt tggtcagtag ttgttgctgt tcttacatat ccgggcattc taccttaccc
     3421 aacgcttccc aataattgct ctgattatta cgttccatcg ccacgatggc ctcgcgtata
     3481 tgttgtttat tgttttcatc tgacaacatt aacttttccc acgcttcatc tgataatgac
     3541 gcatgtggcg tgcaaatttt acgtaaatct tgtaataccg tggttttctc ttttgcggac
     3601 tgcagtgagc taaaaggcga ctcggcgtat acgatgcctg gcatagcgaa aaaagtgaaa
     3661 ataagcagta gtgtcgttgg tttcatggat tcaacctttt acaaatgtgt atcgaatgaa
     3721 gcataagtgt agtttgcttt catcgctctc atcatgctgt acgaaacgcg cttgcgtggt
     3781 cagtaagaag tgccagactt tatattccac ttttattcct ttttattctt atcgatagcg
     3841 tttcgttttt taaaccgcag cgaccttacc gc
//
LOCUS       DAZSNO010000249         3866 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZSNO010000249 DAZSNO010000000
VERSION     DAZSNO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.252"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(102..1460)
                     /gene="plaP"
                     /locus_tag="ACHWN2_004009"
     CDS             complement(102..1460)
                     /gene="plaP"
                     /locus_tag="ACHWN2_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR8933311.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1450..1512)
                     /gene="yoeI"
                     /locus_tag="ACHWN2_004010"
     CDS             complement(1450..1512)
                     /gene="yoeI"
                     /locus_tag="ACHWN2_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR8933312.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(1673..2656)
                     /locus_tag="ACHWN2_004011"
     CDS             complement(1673..2656)
                     /locus_tag="ACHWN2_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR8933313.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(2702..3526)
                     /locus_tag="ACHWN2_004012"
     CDS             complement(2702..3526)
                     /locus_tag="ACHWN2_004012"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8933314.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3609..3863)
                     /gene="yoeB"
                     /locus_tag="ACHWN2_004013"
     CDS             complement(3609..3863)
                     /gene="yoeB"
                     /locus_tag="ACHWN2_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR8933315.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1039 a         1009 c          909 g          909 t
ORIGIN      
        1 aaattagaac aaatggttat taatgaggat ttcgataaaa aagaataaaa aaaccggagc
       61 caaatgttca ttcgactccg gtttattatt agaaaagatg attatgctac gtcttcgtac
      121 tgcggaaccg gattacggaa gctcttggtg acgcaagcca ggtaaatcag accgatagca
      181 gcccagatca gacccagaac cattgagctt tcttccaggt taacccacag cgcaccaacg
      241 gtcagcgcac cacacatcgg caggaacaga tactggaagt gatctttcag cgtcttgtta
      301 cgcttctcac ggatccagaa ctgcgagatg accgacaggt taacaaaggt gaacgccacc
      361 agcgcaccaa agttaatcag cgccgttgcc attaccaggt cgaagttgat tgccagcagg
      421 gcaatcgcgc caaccaggat gatgttcatc gccggagtac gccatttcgg gtgtacataa
      481 ccgaagaagc ttttcgggaa cacgccgtca cgacccatta cgtacatcag acgcgctacg
      541 cctgcgtgcg ccgccatacc ggacgccagt acggtaatgg tggagaagat cagcgcgcca
      601 acctggaacg ctttacccgc aacgtacagc atgatttcag gctgtgacgc atccggatct
      661 ttaaagcgag agatatccgg gaagtacagc tgcaggaagt aagttgcaaa gatgaagatc
      721 atgccgccaa tcagcgcggt caggaaaatc gcacgcggga tcacgcgctc tgcatctttg
      781 gtttcttccg acagattgct gatgccgtca aagccggtaa aggagaagca caggattgtc
      841 gccccggtaa tcatcgggat aacatgcgca tcgccagacc agaacggacg cgtgctcgcc
      901 agcgtaccag cgccttcacc ttcaaatacg ccataaacaa ccatgcccag aatcaccgcg
      961 atcagcacta cctgcaacac gacgattaca gtgttgacgt tcgctacgga tttcagacta
     1021 cgcaggttaa aggcggtcat aaaggccacc agcgccacca caaacatcca cgatggaatg
     1081 gaaggcacca gagcttcaaa atagattttc gccagcagaa tgttgatcat cggcgcgaac
     1141 agatagtcga gcagagaaga ccaacccacc ataaagccga cagtcgggct aatggatttc
     1201 tgggcgtaag tgtatgcaga gccagcagaa ggatagcggc gaaccagctt cccgtagctc
     1261 agagccgtaa acaggatcgc aatcaatgcg aacgcatagg ctgtcggcac atgaccatcc
     1321 gtgaggcctg aaacgatacc aaatgtatca aacagcgtca tcggctgcat ataggctaga
     1381 cccatcatta caaccggaac taacgtaagc gttttacgca attccacgcg agaggtgttt
     1441 ggagtaacgt tatgcgacat ggttattctc ctttacggtg ataaccgtcg cgtaagcaaa
     1501 aaattgcccc atttttttgg attcctcagc gacaacaact gtcgattttt agtaaatatc
     1561 tatccggtac gaagcccggc ctcttggtat gaatgattgg tttgaagcaa aaaataaccg
     1621 acgctaatta aacgtcggtt tttaatcatt ttttgacagc ggcgcattgt gcctaaatgg
     1681 gggggaaatg acaagagaat gagaggcttg tcataataat ttttctttga atggctgatt
     1741 ttccgtcatc agattttgca taaacaccgg cgatcgcact atttgctaaa atttcatctc
     1801 gccaccagcc gctggcaagc cctgcggtct gttcattcca gccaatccag acaggttcaa
     1861 agagagtttg ggcgaccact ttcttctcga ttaacgcgcc actatcgaga aaacgttgcg
     1921 ccagataccg tggtaaatag ccgcagccaa ggccgctaat ttgcagctcc agtttggttt
     1981 taaaatcgaa aacggtaatg gcttcttgtt cgtcgagaag ttgtgaggcc gtagaagcgc
     2041 ctgcctgcgc agtatccccc accacaatcg cccgatatcg tttaatgata cgacggttta
     2101 acggttcttc ttcctgggct aacggatgat gaggcgcgac cgcaaagact tgttcaagat
     2161 cgcccaaccg cgagaaacca aactcactgg aagatggtgg ctcatgcatc gcgccaacga
     2221 taatatccgc tcgcccctga gtcagggcat cccaggaacc accgagtacg ccattgataa
     2281 acttcaggcg cgtcacgcta tgatgttgat aaaaagcttc aatgagaggc gcaagcagag
     2341 aaaaaggaaa cgtgtcgtcc acgccaatca ccagctcgtt ttcccagcct tcatgcagtt
     2401 tgatcgcctg cttttccagt tctcgcacgg tatgcagaac ttcccgccct ttctctaata
     2461 acatttttcc ggtgcgggtg aatttagcgc ggtgtccgct acgatcaagc aattgaatat
     2521 ttaggtcgct ttccagcttg tgaacggtgt aactcagagc ggacggcgtc ttgtagagtt
     2581 tggctgacgc cgccgcaaaa ctgccctctt tttctatggc atcgaggatc atcaacacat
     2641 ccagcagtgg tttcatacac gcccccttgc agtgcatgcg gcgtcctgct ggcgtgatcg
     2701 tttactccaa cggcattacc agcggatcgg gatactggta ttcaaatccc agttcattac
     2761 aaatccgact gccatcaata atcttacctt tgccgctgtc cagactattt ctgaactgcg
     2821 gaggttgcag ccccagtaaa cgggccatct gcggatagaa aacattacgc gcagggtgag
     2881 ctggcgcaca tatattatag atgtgtccgc ctttaggtgc ctgtaacaac agagtgatag
     2941 cgccaataac atcttctaaa tggactaaat taacaccatg ttcaccatca ggggcggttt
     3001 ttccggcaaa gaagcgtccc ggatgacgtc ccggtccgac caggcccgca agacgcagaa
     3061 tatcgaccga agtaccgggt aaattgtgca gccagtcttc gagttcttct aatactcgtc
     3121 cactgttggt taccggatta cgcggggtag tttctttaac cgtgccttgc gcgtcgccat
     3181 agacagatgt tgagctggta aaaataattc ggggaatacg atgggccagc gcgctatcca
     3241 ctaactcttg taccgcttgt aaatagaact catcgccggg gccgctacga cgtgccggaa
     3301 gcgtaatgac cagcgcatcg gcatccataa gggcatccag atcgtcagaa tcgcaaacta
     3361 actcaggctc catgcgaagc agatagctat caatgccact cattcgggcc gcttcgacac
     3421 catcttgtgt ggttttactc ccggtgactt gccagcctcg cgctgaaagt gacatcgcca
     3481 gcggcatacc taaccaccct aacccgacaa ttgcgacctt tttcatccgt tatctcctga
     3541 tacaccttag atctataagg ctacgctagc gtatcaaaac tgacaattca ttctatgaat
     3601 gaatctgttc aataatgata acgacatgct gcaatgagca gtgaatcatc agtaaccgcg
     3661 tataccagac ggtgctcctc tgtaatgcgt cgggaccaga aacctgacaa attatgtttc
     3721 aggggttctg gtttcccctt accttcaaat ggtgttctgc gggtatcttt gataagttca
     3781 ttgatctttt taacaattcg cttatctgtt tcctgccagt acagataatc atcccatgat
     3841 tcctcagacc agattagttt cactca
//
LOCUS       DAZSNO010000250         3841 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZSNO010000250 DAZSNO010000000
VERSION     DAZSNO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.253"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            138..2417
                     /gene="ydeP"
                     /locus_tag="ACHWN2_004014"
     CDS             138..2417
                     /gene="ydeP"
                     /locus_tag="ACHWN2_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR8933316.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2665..2868
                     /gene="safA"
                     /locus_tag="ACHWN2_004015"
     CDS             2665..2868
                     /gene="safA"
                     /locus_tag="ACHWN2_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR8933317.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            2936..3697
                     /gene="ydeO"
                     /locus_tag="ACHWN2_004016"
     CDS             2936..3697
                     /gene="ydeO"
                     /locus_tag="ACHWN2_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR8933318.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1078 a          841 c          883 g         1039 t
ORIGIN      
        1 taacgataat agattaaaaa tagaaagaga gtagtccctt actcttatat atccatgttg
       61 gcgataatcc ctttgtatgt actgtgcatc atcgctatta caaatcctaa taattcattt
      121 ccacacagga taagtagatg aagaaaaaaa ttgaatccta ccagggtgct gcaggtggtt
      181 ggggtgctgt taaatccgta gcgaatgcag tacgtaagca gatggatata cgccatgatg
      241 ttattgccat gtttgacatg aataagccag agggctttga ctgtccgggt tgtgcatggc
      301 cagatcctaa gaacagtgcg tcattcgaca tttgtgaaaa cggcgcaaaa gcaatcgctt
      361 gggaagtcac ggataagcag gtaaatgcct ctttctttgc tgagaatacg gttcaatcat
      421 tacttacctg gggagatcac gagcttgagg ctgcggggcg tctcactcag cctttgaaat
      481 atgatgccgt cagcgactgt tacaagccat taagctggca acaagctttc gacgaaattg
      541 gcgcacgcct tcaaagctat agtgatccca atcaggttga attctatact tcgggccgca
      601 cttccaatga agctgccttt ctttatcagc tttttgcccg tgaatacggg agcaataact
      661 ttcccgactg ctccaacatg tgccatgaac cgacaagcgt gggtttggca gcgagtatcg
      721 gtgtaggtaa agggaccgtg ttgctggaag actttgagaa gtgcgattta gtcatttgca
      781 ttgggcataa ccctggtaca aaccaccctc gcatgctgac ttcgttgcgc gctttagtga
      841 aacggggagc gaaaatgatc gccatcaatc ctctacagga acgtggcctg gagcgattta
      901 ccgcaccgca aaacccgttt gaaatgctga cgaactctga gactcagttg gccagtgcct
      961 actacaacgt gcgcattggt ggcgatatgg cgttgctcaa ggggatgatg cgcctgttaa
     1021 ttgagcgcga tgatgctgca agtgccgcag gtcggccctc attgcttgat gacgaattta
     1081 ttcaaacgca taccgtcggc tttgacgagc tacgccgtga cgttctcaat tccgagtgga
     1141 aagatatcga acgtatttct ggactaagtc agacacaaat cgccgaactg gctgacgcat
     1201 atgccgctgc cgaacgcacc attatctgtt acggaatggg gatcactcag cacgaacatg
     1261 gtacccagaa cgtgcagcaa ctggtcaatt tgctgttgat gaaaggtaac attggcaagc
     1321 ctggtgcggg tatctgccca ctacgtggtc actctaatgt acagggcgac cgaaccgtcg
     1381 gtatcaccga gaaaccgtct gcagagtttc tggctcgcct gggtgagcgc tatggcttcc
     1441 ccccacctca tgcacctgga catgctgcaa ttgccagcat gcaagcaata tgtacggggc
     1501 aggctcgagc attgatctgc atggggggca actttgcact ggcaatgcca gatcgggaag
     1561 cgagcgctgt accgttaacg caattagatt tggcggtaca cgtagccact aagcttaacc
     1621 gctctcatct gctgaccgca cggcatagct atattctgcc ggttctgggg cgtagcgaga
     1681 ttgatatgca aaaaagcggt gcgcaggcgg taaccgttga ggattcaatg tcgatgattc
     1741 atgcctcgcg tggcgtgtta aaacccgccg gtgtaatgct gaaatcagag tgtgcagtgg
     1801 tcgcgggaat cgcgcaggca gcactacccc agagcgtggt agcctgggag tatctggtgg
     1861 aagattatga tcgcattcgc aatgacattg aagctgtgct gccagagttc gccgactata
     1921 accagcgcat ccgtcatccc ggtggttttc acctgataaa tgcagctgct gaaaggcgct
     1981 ggatgacgcc gtcaggtaag gctaatttca ttaccagcaa agggctgtta gaagatccct
     2041 cttcagcgtt taacagtaag ctggtcatgg cgacagtacg cagccacgat cagtacaaca
     2101 cgacgattta tggtatggat gatcgctatc gaggggtatt cggtcaacga gatgtggtct
     2161 ttatgagtgc taaacaagct aaaatttgcc gtgtaaaaaa cggcgaaaga gttaatctta
     2221 ttgcgcttac gccagacggt aagcgcagct cacgccgcat ggatagatta aaagtggtca
     2281 tttaccctat ggctgaccgc tcactggtga cctattttcc agaatcgaat cacatgctaa
     2341 cacttgataa ccacgatcca ttaagtggta ttcctggcta taaaagtatt ccggttgaat
     2401 tagaaccatc aaattaatgc ctcttctcat ttcttctgct gtcatccgca cagcagaaga
     2461 attcctcatt gactattatt gcgcaatttg ctcacatgga ttaaattaaa ctacatacta
     2521 taagatataa acttctgcct acagctgtaa gaaactccgc tcagtactga agcaccagtc
     2581 ctatttcttc ttttctccag cctgttatat taagcatact gattaacgac ttttaacgtt
     2641 atccgctaaa taaacatatt tgaaatgcat gcgaccacag tgaaaaacaa aatcacgcaa
     2701 agagataact ataaagaaat catgtctgta attgtggttg tcttattact gacacttacg
     2761 ttgatagcca ttttttcggc aattgatcag ctgagtattt cagaaatggg tcgcattgca
     2821 agagatctta cacatttcat tatcaatagt ttgcaagact ggaaataaac agcaaattat
     2881 aaatgtgaaa tgtttaaaag gtatcgataa aactttattg ttttaaggag ataaaatgtc
     2941 gctcgtttgt tctgttatat ttattcatca tgctttcaac gctaacattt tagataaaga
     3001 ttacgccttc tccgacggcg agatcctgat ggtagataac gctgttcgta cacattttga
     3061 accttatgag cggcatttta aagaaatcgg atttaatgaa aataccatta aaaaatatct
     3121 acaatgcact aacatccaga cagtgacggt gcctgttcct gcgaagtttt tacgtgcttc
     3181 aaatgtaccg actggattgc ttaatgaaat gattgcttat ctcaactcgg aagaacgcaa
     3241 tcatcataat ttttcagaac ttttgctttt ttcttgcctg tctatttttg ccgcatgcaa
     3301 aggtttcatt acactattaa ctaacggtgt gctatccgtt tccgggaaag tgagaaatat
     3361 tgtcaacatg aagctggcgc acccatggaa gctgaaagat atttgtgact gcctgtacat
     3421 cagtgaaagc ctgttgaaga aaaaactaaa gcaagagcaa acgacattct cacagattct
     3481 attagatgcc agaatgcagc acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa
     3541 aattgccgaa caatgtggtt atgccagtac atcttatttt atttatgcgt tccgcaaaca
     3601 tttcggcaac agtccgaaga gagtttctaa ggagtaccgt tgtcaaaggc acacgggtat
     3661 gaatacgggc aacacgatga gtgctttagc tatttgatta tttgctaacg agtagtcaac
     3721 cacgcacgct acgtaagaat tatatggggc agccattccc tgccccgcgt tgtttttagg
     3781 cgatatattt attgaaataa ataagtgaca tccatcacat atttatgcac ttgcataacc
     3841 t
//
LOCUS       DAZSNO010000251         3806 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZSNO010000251 DAZSNO010000000
VERSION     DAZSNO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.254"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            437..2089
                     /gene="ushA"
                     /locus_tag="ACHWN2_004017"
     CDS             437..2089
                     /gene="ushA"
                     /locus_tag="ACHWN2_004017"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
                     /protein_id="HFR8933319.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="ACHWN2_004018"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="ACHWN2_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="ACHWN2_004019"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="ACHWN2_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3595)
                     /locus_tag="ACHWN2_004020"
                     /pseudo
     CDS             complement(2596..3595)
                     /locus_tag="ACHWN2_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAZSNO010000252         3770 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZSNO010000252 DAZSNO010000000
VERSION     DAZSNO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.255"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            2..1051
                     /gene="tsuA"
                     /locus_tag="ACHWN2_004021"
     CDS             2..1051
                     /gene="tsuA"
                     /locus_tag="ACHWN2_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR8933320.1"
                     /translation="MILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLI
                     AISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEG
                     LIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVLLVI
                     TLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEAT
                     GRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRAADA
                     QTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVY
                     VRPQRKARLATAAAN"
     gene            1065..1292
                     /gene="tsuB"
                     /locus_tag="ACHWN2_004022"
     CDS             1065..1292
                     /gene="tsuB"
                     /locus_tag="ACHWN2_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR8933321.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1335..2759)
                     /gene="sbcB"
                     /locus_tag="ACHWN2_004023"
     CDS             complement(1335..2759)
                     /gene="sbcB"
                     /locus_tag="ACHWN2_004023"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR8933322.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            2941..>3770
                     /locus_tag="ACHWN2_004024"
     CDS             2941..>3770
                     /locus_tag="ACHWN2_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8933323.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
BASE COUNT          896 a          887 c          992 g          995 t
ORIGIN      
        1 aatgatatta agcgggctaa tttgtggtgc tctgctggga tttgtcatgc agcgtgggcg
       61 tttttgcctg actggtggtt ttcgcgatat gtatatcgcg aaaaataatc gcatgtttta
      121 cgccttgctg attgcaattt cggcacaaag cgtgggggtt tttgcgttaa ttcaggcggg
      181 cctactgact tacgaagccg gggcgttccc gtggcttggt actgttatag gtgggtatct
      241 tttcgggctg ggaattgttc tggcgggtgg atgtgccacc gggacctggt atcgcgcggg
      301 tgagggattg atcggcagtt ggatcgcgct tttcacttat atggtgatga gtgcggtgat
      361 gcgttctcca cacgccagtg gtttaaatca aaccttgcag cactacacta ctgaacataa
      421 ctctattgct gagactttta atttgtctgt gtggccgttg gttaccgttt tgctggtgat
      481 aacgctctgg gtggtgatga aagagttgaa gaagccaaaa ctcaaagtcg cgaccttacc
      541 gccgcgccga accgggatcg ctcatattct gtttgaaaaa cgctggcatc cctttgtcac
      601 tgctgtactc atcggtttga ttgcgctttt agcctggccc ctgagtgaag caaccggacg
      661 catgtttggg ttaggagtca cttccccaac ggccaatatt cttcaatttc tggtcgcggg
      721 tgacgtgaaa tacattaact ggggcgtttt cctggtgtta gggatcttcg tggaggcatt
      781 tattgcagcc aaagcgagcc gtgagttccg cgttcgcgca gctgatgcac aaacaacatt
      841 acgtagcggg ttaggtggtg tactgatggg cttcggtgcc agtattgcag gtggttgctc
      901 tatcggtaat ggactggtta tgactgcaat gatgacctgg cagggctgga ttggccttgt
      961 atttatgatt ctcggagtct ggactgcgtc ctggcttgtg tatgttcgac cgcagcgtaa
     1021 ggcgcgactg gcaactgctg cggcaaatta agtaaggtgt aaagatggcg attaaaaagc
     1081 tggatgtggt gacgcaggtc tgtccgtttc cactcataga agcaaaagca gcactggcag
     1141 aaatggccag cggcgatgag ttggtaattg agtttgattg cacccaggca actgaagcaa
     1201 tcccacagtg ggcggcagaa gaaggacatg ccatcaccga ttatcagcag attggcgatg
     1261 cagcgtggag catcaccgtt caaaaagcct gactcaacat tgtcctccgc cgtaccagcg
     1321 gcggcggctt caaattagac gatctcttcc gcgtactgcc aaagtgcttt taacagcgcc
     1381 actttctctt tgtcatcggc atattgttgt accagcatct gcaattcatc agcataaccc
     1441 tgcaaaaact ctggcgtgaa gacctggtgg cggtgctcca gccagcgttg ctgctcggca
     1501 taatccagcg tccccgggaa gttgcgtgcc cgataattga acaacaactt ttcaattcgt
     1561 ttatcaacaa aagtgatatc cagtgccggt aaattacgcg gctcggtttc cagcacaatt
     1621 ttcattgctg cacgatcggc gtcactgaaa aagccattat aaagctgtgc atccacatta
     1681 tctgaaggtg taaacggttc ggcttccgcg aatatcgcta ccactttttc acgcacttgc
     1741 ggattttcac gcagaatttt taggttatcg aggcaatgct gacgattaat tcccagtcgg
     1801 tcggcatctt ccgggcgtag cgtattcgcc tgggccagta ccggacattt attgatatgc
     1861 accagcttaa ccggaacggc ggcgttatcg ccaagatcgg cttttgcggt atataaacgc
     1921 tcgcgcaatg tgtcgatatc cagctccagt aatggcgaaa tgtctcctgc caaatccgcc
     1981 ataattacgg cattgcgatt ttcaggatgc cacgccagcg gtgccaccca gctggtattg
     2041 ccacgccatg ccccaaacat tccggaaacg tgagccaggg gtttcatctg cggaacatca
     2101 atcaacgcca tcagtttgtg tttattacga tgggtaaaga gataatcaaa caggcgtggc
     2161 tgacgcgttt ttaccagttt cgccatcgca atagtggcgt acacatcagc catcgcatcg
     2221 tgggcgttgc tatgttcaat accattcgct ttggttaaat gctcaaggcg aaagctcggt
     2281 agaccgtcat cattttcagg ccagtttatt ccttccgggc gcagggcata acaggcacgc
     2341 ataacatcca gtaaatccca gcgcgagtta tcatgctgcc agctccaggc gtaaggatcg
     2401 tagaaattac gataaaaaac gttgcgtgtg acttcgtcgt cgaaacgcac attgttgtag
     2461 cccagaatac aggtcttcgg tacggtaaaa agcgagtgaa tacgggcggc aaacgcggct
     2521 tcgttttctc ctttcgcccg tgcctcctgc ggggtaatac cggtaattaa tactgctcca
     2581 ggccggggta aatagtcatc cgcgggcttg cagtaaaaga cttcgggttc gccgatgaca
     2641 ttgaattcgt catcggtgcg aatggctgcg aactgtgcag ggcgatctaa cgcggggtgc
     2701 gtgccaaagg tttcgtaatc gtgaaagagg aaggtaggtt gtttatctgt gtctgtcatt
     2761 gtctagcatt gtccactgta gttaattaac tgtctgttat aactcgaaaa gcaacccttt
     2821 tttcacctga gtatgtctac gaacgtcaaa tatagttgtt tactgtgcgc tgttgtccat
     2881 gtgatattgt gtaccagatg tgtgccaaac tattatttct gagcgtacca aatattattt
     2941 atggctataa gcgacacaaa actgcgtact atttatggta aaccatattc gggcccacaa
     3001 gaagtggctg atgccgatgg cctcagcgta cgaatttcac cgaagggggt catccagttc
     3061 cagtaccgct atcgctggca tggcaagcct aatcgacttg ggcttggtcg atacccatcc
     3121 ctgtctttga aggatgccag acagatcact gctgacttgc gaaagctcta tttctcagga
     3181 acggatccac gcacctattt tgaagagaag gtggagaact ccatgacggt cgcccagtgt
     3241 ctcgactact ggttcgacaa ctacgtctct acaactctca gagaaaagac ccaggcactt
     3301 tatcgatcaa cggttatgaa gcgcatgcat gacgccttcc ctaatcgtcc ggcatcttct
     3361 atcacggtta agcaatgggt tgacctgctt accgaagaag aaaaagataa tccacgccga
     3421 gcaaggcagg tgctaagtca actaagatca gcaattagtt ggtgcatgcg gcgtcagttg
     3481 atagatagtt gcgcaattat gagcatccag ccaagggact tcggctcccg cgctgaggta
     3541 ggggatcggg tactgtcgta tcacgaactg gctaagattt ggcttgctat tgaaagaagc
     3601 cgtgcgtcta cgtcaaataa gctccttcat cagatgctta tgctgtgggg ggcgaggctc
     3661 tcagagctta ggctggcaac aaagacagaa tttgacctgc tggacaacgt atggaccgta
     3721 ccgaaagagc atagcaagat gggtaatgtt atccgccgtc caatcttcga
//
LOCUS       DAZSNO010000253         3730 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZSNO010000253 DAZSNO010000000
VERSION     DAZSNO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.256"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..520
                     /locus_tag="ACHWN2_004025"
     rRNA            <1..520
                     /locus_tag="ACHWN2_004025"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            606..681
                     /locus_tag="ACHWN2_004026"
     tRNA            606..681
                     /locus_tag="ACHWN2_004026"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:640..642,aa:Glu,seq:ttc)
     gene            875..>3730
                     /locus_tag="ACHWN2_004027"
     rRNA            875..>3730
                     /locus_tag="ACHWN2_004027"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          975 a          830 c         1140 g          785 t
ORIGIN      
        1 tgtgccttcg ggaaccgtga gacaggtgct gcatggctgt cgtcagctcg tgttgtgaaa
       61 tgttgggtta agtcccgcaa cgagcgcaac ccttatcctt tgttgccagc ggtccggccg
      121 ggaactcaaa ggagactgcc agtgataaac tggaggaagg tggggatgac gtcaagtcat
      181 catggccctt acgaccaggg ctacacacgt gctacaatgg cgcatacaaa gagaagcgac
      241 ctcgcgagag caagcggacc tcataaagtg cgtcgtagtc cggattggag tctgcaactc
      301 gactccatga agtcggaatc gctagtaatc gtggatcaga atgccacggt gaatacgttc
      361 ccgggccttg tacacaccgc ccgtcacacc atgggagtgg gttgcaaaag aagtaggtag
      421 cttaaccttc gggagggcgc ttaccacttt gtgattcatg actggggtga agtcgtaaca
      481 aggtaaccgt aggggaacct gcggttggat cacctcctta ccttaaagaa gcgttctttg
      541 tagtgctcac acagattgtc tgatagaaag tgaaaagcaa ggcgtcttgc gaagcagact
      601 gatacgtccc cttcgtctag aggcccagga caccgccctt tcacggcggt aacaggggtt
      661 cgaatcccct aggggacgcc acttgctggt ttgtgagtga aagtcacctg ccttgatatc
      721 tcaaaactca tcttcgggtg atgtttgaga tatttgctct ttaaaaatct ggatcaagct
      781 gaaaattgaa acactgaaca acgaaagttg ttcgtgagtc tctcaaattt tcgcaacacg
      841 atgatgagtc gaaagaaaca tcttcgggtt gtgaggttaa gcgactaagc gtacacggtg
      901 gatgccctgg cagtcagagg cgatgaagga cgtgctaatc tgcgataagc gtcggtaagg
      961 tgatatgaac cgttataacc ggcgatttcc gaatggggaa acccagtgtg attcgtcaca
     1021 ctatcattaa ctgaatccat aggttaatga ggcgaaccgg gggaactgaa acatctaagt
     1081 accccgagga aaagaaatca accgagattc ccccagtagc ggcgagcgaa cggggagcag
     1141 cccagagcct gaatcagtgt gtgtgttagt ggaagcgtct ggaaaggcgt gcgatacagg
     1201 gtgacagccc cgtacacaaa aatgcacata ctgtgagctc gatgagtagg gcgggacacg
     1261 tggtatcctg tctgaatatg gggggaccat cctccaaggc taaatactcc tgactgaccg
     1321 atagtgaacc agtaccgtga gggaaaggcg aaaagaaccc cggcgagggg agtgaaaaag
     1381 aacctgaaac cgtgtacgta caagcagtgg gagcacgctt aggcgtgtga ctgcgtacct
     1441 tttgtataat gggtcagcga cttatattct gtagcaaggt taaccgaata ggggagccga
     1501 agggaaaccg agtcttaact gggcgttaag ttgcagggta tagacccgaa acccggtgat
     1561 ctagccatgg gcaggttgaa ggttgggtaa cactaactgg aggaccgaac cgactaatgt
     1621 tgaaaaatta gcggatgact tgtggctggg ggtgaaaggc caatcaaacc gggagatagc
     1681 tggttctccc cgaaagctat ttaggtagcg cctcgtgaat tcatctccgg gggtagagca
     1741 ctgtttcggc aagggggtca tcccgactta ccaacccgat gcaaactgcg aataccggag
     1801 aatgttatca cgggagacac acggcgggtg ctaacgtccg tcgtgaagag ggaaacaacc
     1861 cagaccgcca gctaaggtcc caaagtcatg gttaagtggg aaacgatgtg ggaaggccca
     1921 gacagccagg atgttggctt agaagcagcc atcatttaaa gaaagcgtaa tagctcactg
     1981 gtcgagtcgg cctgcgcgga agatgtaacg gggctaaacc atgcaccgaa gctgcggcag
     2041 cgacgcttat gcgttgttgg gtaggggagc gttctgtaag cctgtgaagg tgtgctgtga
     2101 ggtatgctgg aggtatcaga agtgcgaatg ctgacataag taacgataaa gcgggtgaaa
     2161 agcccgctcg ccggaagacc aagggttcct gtccaacgtt aatcggggca gggtgagtcg
     2221 acccctaagg cgaggccgaa aggcgtagtc gatgggaaac aggttaatat tcctgtactt
     2281 ggtgttactg cgaagggggg acggagaagg ctatgttggc cgggcgacgg ttgtcccggt
     2341 ttaagcgtgt aggctggttt tccaggcaaa tccggaaaat caaggccgag gcgtgatgac
     2401 gaggcactac ggtgctgaag caacaaatgc cctgcttcca ggaaaagcct ctaagcatca
     2461 ggtaacatca aatcgtaccc caaaccgaca caggtggtca ggtagagaat accaaggcgc
     2521 ttgagagaac tcgggtgaag gaactaggca aaatggtgcc gtaacttcgg gagaaggcac
     2581 gctgatacgt aggtgaagcg acttgctcgt ggagctgaaa tcagtcgaag ataccagctg
     2641 gctgcaactg tttattaaaa acacagcact gtgcaaacac gaaagtggac gtatacggtg
     2701 tgacgcctgc ccggtgccgg aaggttaatt gatggggtta gcgcaagcga agctcttgat
     2761 cgaagccccg gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt
     2821 cgggtaagtt ccgacctgca cgaatggcgt aatgatggcc aggctgtctc cacccgagac
     2881 tcagtgaaat tgaactcgct gtgaagatgc agtgtacccg cggcaagacg gaaagacccc
     2941 gtgaaccttt actatagctt gacactgaac attgagcctt gatgtgtagg ataggtggga
     3001 ggctttgaag tgtggacgcc agtctgcatg gagccgacct tgaaatacca ccctttaatg
     3061 tttgatgttc taacgtggac ccgtgatccg ggttgcggac agtgtctggt gggtagtttg
     3121 actggggcgg tctcctccta aagagtaacg gaggagcacg aaggttggct aatcctggtc
     3181 ggacatcagg aggttagtgc aatggcataa gccagcttga ctgcgagcgt gacggcgcga
     3241 gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaagggc catcgctcaa
     3301 cggataaaag gtactccggg gataacaggc tgataccgcc caagagttca tatcgacggc
     3361 ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagtagg tcccaagggt
     3421 atggctgttc gccatttaaa gtggtacgcg agctgggttt agaacgtcgt gagacagttc
     3481 ggtccctatc tgccgtgggc gctggagaac tgaggggggc tgctcctagt acgagaggac
     3541 cggagtggac gcatcactgg tgttcgggtt gtcatgccaa tggcactgcc cggtagctaa
     3601 atgcggaaga gataagtgct gaaagcatct aagcacgaaa cttgccccga gatgagttct
     3661 ccctgaccct ttaagggtcc tgaaggaacg ttgaagacga cgacgttgat aggccgggtg
     3721 tgtaagcgca
//
LOCUS       DAZSNO010000254         3729 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZSNO010000254 DAZSNO010000000
VERSION     DAZSNO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.257"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..176)
                     /locus_tag="ACHWN2_004028"
                     /pseudo
     CDS             complement(1..176)
                     /locus_tag="ACHWN2_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            174..498
                     /locus_tag="ACHWN2_004029"
                     /pseudo
     CDS             174..498
                     /locus_tag="ACHWN2_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(528..3440)
                     /locus_tag="ACHWN2_004030"
     CDS             complement(528..3440)
                     /locus_tag="ACHWN2_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR8933324.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKVFYERSEKSVGYWVGGIRRAAQKA"
     gene            complement(3497..>3729)
                     /locus_tag="ACHWN2_004031"
     CDS             complement(3497..>3729)
                     /locus_tag="ACHWN2_004031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933325.1"
                     /translation="RWHTEFGYLNRGDMFTSEQHRYSNEKKEFQRRVYLRGDAFINLY
                     TNFYSCIMTGLQLLRHFQRIALPAYHPTFFIP"
BASE COUNT          934 a          998 c          917 g          880 t
ORIGIN      
        1 ttttttacca ccgggagttt gggcataacg gtgctttcag taagtacgac aggtactgtg
       61 gaggcacttc cgtaaagaag ggcatcaagc tcctgctccg acatacctgc aggcaaaggc
      121 catgaaagcc ctgatttccg gaagcgagcg aacagtccac aaactgttga acgtggtgtc
      181 agcggcggac agggataagc cctcgtcagc agttccgtca gcactgcgac agcgttcttt
      241 acccggtcaa aacagcgttg cggtgcacca cgcctgacgg atgaactgcg tgctcagggt
      301 taccacttta acgtaaaaac cgtggcggca agcctgcgcc gtcaggggct gagggcaaag
      361 gcctcccgga agttcagccc ggtcagttac cgcgcacatg gcctgcccgt gtcagaaaat
      421 ctgctggagc aggattttta cgccagtggt ccgaaccaga agtgggcggg agacatcacg
      481 tacttacgta cagatgaagt tcggttacat cctgtcagca cggaacctca cgctttttga
      541 gcggcacgac ggatcccccc cacccagtat ccgacagact tttcactgcg ttcgtaaaaa
      601 accttcttcc ctcccagcat atccactaat tccggtgaca tatcctgcag ataagtctgc
      661 aaatcatgcc ccagatcttc cggtgactgc aactcataaa ccgcgcgcca cacatcaccc
      721 ttccttattt taagccccgg gttaagaata tactgtccgt ggttcagcct cagaaaaagc
      781 ttttttccat aaggattttt gctgttaact tcatgttgag agagctggct gctgatataa
      841 ctccttcgtt tgcggcgttc cggcagcaac gcttcaggaa gatgaaccac acactctgcc
      901 agtcgtgccg cactgaaggc ttccgaaaaa aacagagcac ttcccagatg cgtgtggaac
      961 agcgcctgca tcaggatcac cagaaacatt gccatctgcc ggttgtccag tttgatgagg
     1021 cgtccttcca cctgcagtga cagactttca ggtttcagca gggtatacaa ctgcgcactt
     1081 ttctgttgcg cataacgggg cgtactgacc gccgctgaca gaacttgatg ataagccgcc
     1141 agcccgtcat taccggtcag caagggatct gcaccacgct ccagcagtaa ctgaaccagt
     1201 gcaatattac ctgcccgggc tgctgacatc agcggagtaa gattaaacgg cgaacggtga
     1261 tcaacaccat aacgttcaat atcccgcaac acggcaaccg ggttattcat tgagtatggc
     1321 cggaaatact tcagctccag taactgcaga gtcttcgttc tgggctgcct tgtgggctta
     1381 aaatcttccc ggtacagtgc attatacagc gggaacaggg aataaatcag actgtactcc
     1441 agcgcctgca gttgtatggt tttatcggct gtacctgcga ggatatgctg tttccactgt
     1501 cgtgcatcct ctgcggtgat aatctgccat ggcatctcag atgtctgcag gatccggcta
     1561 cggatggtgt ccgcctgctc ctgcttcccc tgtttttcaa gcagatgtgc ggttttctgc
     1621 cactcatcac ggctggaaat ctcttcctgc agattcagag gctcttcctg atgcgtgagc
     1681 gcaagcagac tccacagagg atgctccacc tgctgttcaa caagatacac attgtgttta
     1741 gcacgggtga gcgcaacata cagcgcgttg gtaaaaaatt tatagatttc cgctgaacga
     1801 tcctgcctgt cccgcggacg ggaataacgg gcctcgccct caagatcagc cggtgtaaga
     1861 ccctcgcaaa tatcatcaaa tgcctgccgg gcagcgctta ctatattgta aaggatcacc
     1921 gtttcgtatt ccagcccctt aacctcctga accgaaaaga ccagcggcgt actgaaccag
     1981 catcgcgcct gtgctttctg ttccggatgc atcacaatga ccgctacacg attcgaaagt
     2041 gaggttttgg cattcagttc ctgctttgtc tcttccctat cgtccagcaa ccggatggtc
     2101 ccttctgcct gaccgcagct tctgacaaaa tggtgacttt cccgatcaat agcactaaag
     2161 cggacctgtt tgagtctcag caaccggtta gccagtgcag tgacgtggtg cccgttacgg
     2221 taattcgcct gcaggatatt aacggttgtg tcgtttccct gttgctgccg gaaaaagaga
     2281 cttttgagcg aactccatga gaaaaagctc ggatgaacta tctgattggc atcaccacag
     2341 agtaaaaact gaccgggatg acgtagcgtt ttcagtacca gttgcagttg aacaggcgtc
     2401 atatcctgaa cttcatcaac aaaaatgaca tcataacggg gagttgccaa cgaaagatac
     2461 tgatgactga gcagattact gtcatactga tgcgactgtt tcagccatgc gatataacgt
     2521 tcaaacagga catagaccgt cgggcggtcc tccatgccat agagcgactg ccgtatcccc
     2581 agtgacagat acgcctcccg ggacagcggc ccattcccgg aggcaaccgc accgatcacg
     2641 cccctgaact cctcatacag ggtatgggca gcccccagtg cggcgatagc cctgttgcgg
     2701 ggcagccagt cgctgaacgc actgaaagtc acctccctgc cttccggaat gcgcagagtt
     2761 tccagaaatt ccgtcagcga aagaaagtca atattctgta cctcactccc ttcccctgaa
     2821 gcgtcataga gagtacgcgc tttttccacc agaaatgagg aaaggctgag atacagtata
     2881 tcaccagcag cctgcttcat tttttccagt accagcgccg tcttaccact tcccgccgag
     2941 ccaacaatca ccagcggtag cgggtgttcg tacagtgcct gctgatcatc atcaaaggac
     3001 agcattttat ccaggcggtg gaaacgccca tgagacggat tgatatacgt cagcgcttca
     3061 gtcgcgatat caacaggatc cggcactggt tgttgttgta agcgcccttc atcaatcacc
     3121 acgtttctgc gaagaaaacg tgaagtgtta taggcatgat tgcgaatata ttccagaaca
     3181 aggcagatag tttcaccgtg gtagcgatac agcgaaaaca gtaatcggtc ccggatactg
     3241 agccgggcgc ggtaaagatt ggtgtcaatt ttacgaacat cagcctgaac aaagttatcg
     3301 gtggcaatag cctgtgcaaa ttttttataa cccgggatcg tgtcaggatt cagagtatga
     3361 taaagacaga ttttcatggc tgccggtttc cgtcgtgcaa aaaacatgct gattttacga
     3421 aggaatgcct tactgaacaa ccttgcccgg aatacccccc gaaatcagat ggaacaatca
     3481 gaaaaatgct ctgcagttat ggaataaaga aggtggggtg gtacgccggg agggcgatcc
     3541 gctggaaatg gcgcagtagc tgcaggcctg tcatgatgca ggaatagaag ttcgtgtaca
     3601 ggttaatgaa agcgtcaccc cgtaaataaa ctctgcgctg aaattccttt ttttcattgg
     3661 agtacctgtg ttgttctgag gtgaacatat cacctctgtt caggtagcca aattcagtgt
     3721 gccacctca
//
LOCUS       DAZSNO010000255         3709 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZSNO010000255 DAZSNO010000000
VERSION     DAZSNO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.258"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            319..1470
                     /locus_tag="ACHWN2_004032"
                     /pseudo
     CDS             319..1470
                     /locus_tag="ACHWN2_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            complement(1500..1928)
                     /locus_tag="ACHWN2_004033"
                     /pseudo
     CDS             complement(1500..1928)
                     /locus_tag="ACHWN2_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(2443..3486)
                     /locus_tag="ACHWN2_004034"
     CDS             complement(2443..3486)
                     /locus_tag="ACHWN2_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR8933326.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT         1008 a          841 c          867 g          993 t
ORIGIN      
        1 attaatgaga caagtatagt gccgcagata aactctttgg tcacaaagga tgcactgttt
       61 tgacatatta ctttctcatt tgcatgagtt atctttggtg ataataacat tattataata
      121 ctgaacagca tcataacaag aaatataaca ttccagttat aatattcaat aatcaatgcg
      181 cgaggtgcta ctaatggtat tactgcgctt acagatgata tatcagaaag cgcccgtttt
      241 agttttaatg gagggtgatc ttacccacca atagtggaca caggactaag tgagtaaact
      301 ctcaaccaga ggtgactcat gacaaaacca gtatcaatca gcaagaagcc ccgtaaacaa
      361 catacgcctg aatttcgtaa cgaagccctg aaactcgctg aacgcatcgg tgtggccgcc
      421 gcagcccgtg aactcagcct gtatgaatct cagctttatg cctggcgcag taaacagcag
      481 caacaaatga gttcgtcaga gcgcgaaagc gaactggccg ctgaaaatgt ccgccttaaa
      541 cgacaactgg cggagcaggc tgaggaactg tccatcctcc aaaaggccgc gacatacttc
      601 gcgaagcgcc tgaaatgaag tatgtcttta tcgaaaatca tcgggcagag ttcagcatca
      661 aagcgatgtg tcgtgtactt cgggttgccc gcagcggctg gtatgtctgg ctcaggcgtc
      721 gtcaccagat gagcctgcgc caacagtttc ggctcacctg cgatgccgct gtccataagg
      781 cattctttga ggcaaagcag cgatacggtg ctccccgcct tgctgacgaa ctgccggagt
      841 tcaatattaa aaccattgcc gccagcctgc gtcgtcaggg gctgcgggcg aaagccagcc
      901 ggaagttcag cccggtcagc taccgtgcac atggcctgcc cgtattggag aatctgctgg
      961 agcaggactt cagcgccagt ggcccgaaac cagaagtggg cgggtgacat cacgtacttg
     1021 cgtaccgatg agggctggtt gtatctcgca gtagtcatcg acctgtggtc acgcgccgtc
     1081 attggctggt cgatgtcacc gcgaatgaca gcacaactgg cctgtgatgc actgcaaatg
     1141 gcgttgtggc ggagaagacg cccggaaagc gtcattgttc atacggaccg tggtggtcaa
     1201 tactgttcag gggattatca ggcaatgctg aagcgacaca acctgcgtgg cagtatgagt
     1261 gcgaaaggct actgttatga caatgcctgt gtggaaagct tctttcattc gctgaaggtg
     1321 gaatgtatcc acggggaacg ctttagcagc cgggaaataa tgcgggcaac ggtgtttaat
     1381 tatatcgagt gtgattacaa tcgctggcgt cgtcacagtg cctgtggcgg tctcagcccg
     1441 gaacaatttg aaaaccataa tctcgcttag ggccgtgtcc acattacgtg ggtaggatca
     1501 gtatgggtcg tcttgtattt gtcatactgc ccaatctcac caatcgaaaa tgctcctttt
     1561 ggcaaaggtt caagcaggtc tctgggaatg gaggtcgctg gcgtcggata taaaaaaccc
     1621 tcacggctta gcaaggctgg cgtcactatc ggtgtaacaa tatcaggcgt gcctgcggaa
     1681 cggtccttac catgctgcag taaccggaaa taaacttttg ggcgtaagat gacattgaca
     1741 gtttcgacat cgtctttttc tcccttaaaa aatttattga caatcgcttc gtcaagacgt
     1801 cccgccgcta tcccatgcca gtgagtgaaa ttttgggcgt ctttccgttc aaaacagccc
     1861 tttcctgact cagcatctgc aagcgagttg cgccagtatg aggcaaacct taaagcattt
     1921 tcatccatta ccagtcctgt gactcaatga aatccattga tttatcgaat ataaaaccac
     1981 cgtatggaca actattgtcc atattaaaaa agttggatgc aatgttttgt ttataattcg
     2041 atatcaaatg tactggttgt acgaggttca gaacacaagc acattcaaca gtacgtcagg
     2101 actattgatt tcataacagc gaatacaatt ttttactgtt tataatggct gcctgtgttg
     2161 gacggggata ccagttcaac cgaaaacgcc agaggagggg attacccgct ggcagggtaa
     2221 atctgtggtt tttgatgcca gctataacgt gtttgacgat aaagtattac gaaagaactt
     2281 taatgagttc atggagtaat tcgttaaagt aatttacaac gctatccttt tgcaaaggaa
     2341 actttcagaa aataacccca gggtaatcac ctggggaagg aacacagtgt tattattgga
     2401 taataaatta ttacttgttt taaagttaca ctaatgcaat atctactgat actggatgct
     2461 tgaatgtttc atccctgctg cggaaaaggg caatatccag ggccagatat attcaccgtt
     2521 catatttctg catttctgta caatttcctc agaaagtgtc ttgtcaattc ttttccacag
     2581 gtgctcctgc tgtcttaaaa tactttcgca tatttcgaaa tcatcttcct gaatcagcat
     2641 ttcggcgcat ttaatgattt ctgatcgttc atgcagcacg gaggaatggt tatagagcca
     2701 gtttacaaac agcaatgctt ccggatattg tccccataat ggtgagtatg ttgcatagta
     2761 atcttctgcc gtatccagag agccggagaa gtcatgccac acagcaagtg gaaatggatg
     2821 cagatttccg cacagcattt ctgcgatgta agtgtttgtg gcaaagccat ggcgaaaagc
     2881 tgtggcggcc ttaacatgtt ccccgttcag aaaatgcagg agaccgagtt cataccagta
     2941 aggtgaaaat tcatcagcat gctctttcag tacactgaag gctcgctcat gatcgccggt
     3001 gcgtaacagc tctgaaccca acaaccagcg agccccctga ttatcttcag gattgtatgc
     3061 cagtatttta tcagtgagca taaccgcatc ctgatggcgc tgtaagtgca catttgcgag
     3121 aattgctgca taaagcgcgc gcagataagg ccggttttcg ggatgcatcc agattatttc
     3181 accacagaaa ctttccggga tgatacgatt acctgccgcc agtcctttaa gagcggcgtt
     3241 cagtgcctta cgcggggcat tctgttccag aaatgcatat gccagatgag cgtgtatatc
     3301 aataaagtcc ggctcccgtt gagcaaggcg tcggagtttg ttgatgtaac tcgtctcgct
     3361 gatttcatca gcgtcgtatg catcaagcac ttcttcaaac tcccccagca atgggccaaa
     3421 ccaggtatcc ggaaatacga aataaccctt gttatcgtgc acttcaaagc ataatctatc
     3481 gttcattttc tgagcctgta aaacatgtgg tgatatagtc tgagagcata aaagttgaga
     3541 ttctgtgtat caagtcctgt cttggcaccg aatgaatatt gcttacgaag ccccttcatg
     3601 ctttccttga gaacaataat tttatcttta tgtgaatctc attttttata atgatgtcag
     3661 atgattgaaa tgctaaatta tatctgaatc aactattcct ttgctaaag
//
LOCUS       DAZSNO010000256         3670 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZSNO010000256 DAZSNO010000000
VERSION     DAZSNO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.259"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(41..85)
                     /locus_tag="ACHWN2_004035"
     CDS             complement(41..85)
                     /locus_tag="ACHWN2_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933327.1"
                     /translation="MKRNDKILHYEGLN"
     gene            204..1928
                     /gene="ilvI"
                     /locus_tag="ACHWN2_004036"
     CDS             204..1928
                     /gene="ilvI"
                     /locus_tag="ACHWN2_004036"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR8933328.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1931..2422
                     /gene="ilvN"
                     /locus_tag="ACHWN2_004037"
     CDS             1931..2422
                     /gene="ilvN"
                     /locus_tag="ACHWN2_004037"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR8933329.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVYVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2434..2520
                     /locus_tag="ACHWN2_004038"
     CDS             2434..2520
                     /locus_tag="ACHWN2_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR8933330.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2602..3606
                     /gene="cra"
                     /locus_tag="ACHWN2_004039"
     CDS             2602..3606
                     /gene="cra"
                     /locus_tag="ACHWN2_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR8933331.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          852 a          878 c         1019 g          921 t
ORIGIN      
        1 gagaaattgc tgtaagttgt gggattcagc tgatttatta tcaatttaat ccttcgtaat
       61 ggaggatttt atcgtttctt ttcacctttc ctcctgttta ttcttattac cctgcgttta
      121 tgtctctggc tgccaattgc ttaagcaaga tcggacggtt aatgtgtttt acacattttt
      181 tccgtcaaac agtgaggcag gccatggaga tgttgtctgg agccgagatg gtcgtccgat
      241 cgcttatcga tcagggcgtt aaacaagtat tcggttatcc cggaggcgca gtccttgata
      301 tttacgatgc attgcatacc gtgggtggta ttgatcatgt attagttcgt catgagcagg
      361 cggcggtgca tatggccgat ggcctggcgc gtgcgaccgg ggaagtcggc gtcgtgctgg
      421 taacgtcggg tccaggggcg accaatgcga ttaccggcat cgccaccgct tatatggatt
      481 ccattccatt agttgtcctt tccgggcagg tagcgacctc gttgataggt tacgatgcct
      541 ttcaggagtg cgacatggtg gggatttcgc gaccggtggt taaacacagt tttctggtta
      601 agcaaacgga agacattccg caggtgctga aaaaggcttt ctggctggcg gcaagtggtc
      661 gcccaggacc agtggtcgtt gatttaccga aagatattct taatccggcg aacaaattac
      721 cctatgtctg ccctgagtcg gtcagtatgc gttcttacaa tcccactact accggacata
      781 aagggcaaat taagcgtgct ctgcaaacgc tggtagcggc aaaaaaaccg gttgtctacg
      841 taggcggtgg ggcaatcatg gcgggctgcc atcagcagtt gaaagaaacg gtggaggcgt
      901 tgaatctgcc cgttgtttcc tcattgatgg gaatgggggc gtttccggca acgcatcgtc
      961 aggcactggg catgctggga atgcacggta cctacgaagc caatatgacg atgcataacg
     1021 cggatgtgat tttcgccgtc ggggtacgat ttgatgaccg aacgacgaac aatctggcaa
     1081 agtactgccc aaatgccact gttctgcata tcgatattga tcctacttcc atttctaaaa
     1141 ccgtgactgc ggatatcccg attgtggggg atgctcgcca ggtcctcgaa caaatgcttg
     1201 aactcctgtc gcaagaatcc gcccatcaac cgctggatga gatccgcgac tggtggcagc
     1261 agattgaaca gtggcgcgct cttcagtgcc tgaaatatga cactcacagt gaaaagatta
     1321 aaccgcaggc ggtgatcgag actctttggc ggttgactaa tggggacgct tacgtgacgt
     1381 ccgatgtcgg gcagcaccag atgtttgctg cgctttatta tccattcgac aaaccgcgtc
     1441 gctggatcaa ttccggcggt ctcggcacga tgggttttgg tttacctgcg gcactgggcg
     1501 tcaaaatggc gttgccagaa gaaaccgtgg tttgcgtcac tggcgacggc agtattcaga
     1561 tgaacatcca ggaactgtct accgcgttgc aatacgagtt gcccgtactg gtggtgaatc
     1621 tcaataaccg ctatctgggg atggtgaaac agtggcagga catgatctat tccggccgtc
     1681 attcacaatc ttatatgcaa tcactacccg atttcgtccg tctggcggaa gcctatggtc
     1741 acgtcgggat ccagatctct catccgcatg agctggaaag caaacttagc gaggcgctgg
     1801 aacaggtgcg caataatcgc ctggtgtttg ttgatgttac cgtcgatggc agtgagcacg
     1861 tctacccgat gcagattcgc gggggcggaa tggatgaaat gtggttaagc aaaacggaga
     1921 gaacctgatt atgcgccgga tattatcagt cttactcgaa aatgaatcag gcgcgttatc
     1981 ccgcgtgatt ggcctttttt cccagcgagg ctacaacatt gaaagcctga ccgttgcgcc
     2041 aaccgacgat ccgacattat cgcgtatgac catccagacc gtgggcgatg aaaaagtact
     2101 tgagcagatc gaaaagcaat tacacaagct ggtctatgtc ttgcgcgtga gtgagttggg
     2161 gcagggcgcg catgttgagc gggaaatcat gctggtgaaa attcaggcca gcggttacgg
     2221 gcgtgacgaa gtgaaacgta atacggaaat attccgtggg caaattatcg atgtcacccc
     2281 ctcgctttat accgttcaat tagcaggcac cagcgataag cttgatgcat ttttagcatc
     2341 gattcgcgat gtggcgaaaa ttgtggaagt tgctcgctct ggtgtggtcg gactttcgcg
     2401 cggcgataaa ataatgcgtt gagaatgatc tcagtgcgca atttgcagcc caacatgtca
     2461 cgttgggctt tttttgcgaa atcagtggga acctggaata aaagcagttg ccgcagttaa
     2521 ttttctgcgc ttagatgtta atgaatttaa cccataccag tacaatggct atggttttta
     2581 cattttacgc aaggggcaat tgtgaaactg gatgaaatcg ctcggctggc gggagtgtcg
     2641 cggaccactg caagctatgt tattaacggc aaagcgaagc aataccgtgt gagcgacaaa
     2701 accgttgaaa aagtcatggc tgtggtgcgt gagcacaatt accacccgaa cgccgtggca
     2761 gctgggcttc gtgctggacg cacacgttct attggtcttg tgatccccga tctggagaac
     2821 accagctata cccgcatcgc taactatctt gaacgccagg cgcggcaacg gggttatcaa
     2881 ctgctgattg cctgctcaga agatcagcca gacaacgaaa tgcgctgcat tgagcacctt
     2941 ttacagcgtc aggttgatgc cattattgtt tcgacgtcgt tgccacctga gcatcctttt
     3001 tatcaacgct gggctaacga cccgttcccg attgtcgcgc tggaccgcgc cctcgatcgt
     3061 gaacatttca ccagcgtggt tggtgccgat caggatgatg ccgaaatgct ggcggaagag
     3121 ttacgtaagt ttcccgccga gacggtgctt tatcttggcg cgctgccgga gctttctgtc
     3181 agcttcctgc gtgaacaagg tttccgtact gcttggaaag atgatccgcg cgaagtgcat
     3241 ttcctgtatg ccaacagcta tgagcgggag gcggctgccc agttattcga aaaatggctg
     3301 gaaacgcatc cgatgccgca ggcgctgttc acaacgtcgt ttgcgttgtt gcaaggagtg
     3361 atggatgtca cgctgcgtcg cgacggcaaa ctgccttctg acctggcaat tgccaccttt
     3421 ggcgataacg aactgctcga cttcttacag tgtccggtgc tggcagtggc tcaacgtcac
     3481 cgcgatgtcg cagagcgtgt gctggagatt gtcctggcaa gcctggacga accgcgtaag
     3541 ccaaaacctg gtttaacgcg cattaaacgt aatctctatc gccgcggcgt gctcagccgt
     3601 agctaatccg cgaacaaaaa tacgcgccag gtgaatttcc ctctggcgcg tagagtacgg
     3661 gactggacat
//
LOCUS       DAZSNO010000257         3632 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZSNO010000257 DAZSNO010000000
VERSION     DAZSNO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.260"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..63)
                     /locus_tag="ACHWN2_004040"
     CDS             complement(<1..63)
                     /locus_tag="ACHWN2_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933332.1"
                     /translation="MNLDGVRPYCRIVNKKNESIS"
     gene            complement(313..432)
                     /locus_tag="ACHWN2_004041"
                     /pseudo
     CDS             complement(313..432)
                     /locus_tag="ACHWN2_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            676..1048
                     /locus_tag="ACHWN2_004042"
                     /pseudo
     CDS             676..1048
                     /locus_tag="ACHWN2_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1610..2557)
                     /gene="icsP"
                     /locus_tag="ACHWN2_004043"
     CDS             complement(1610..2557)
                     /gene="icsP"
                     /locus_tag="ACHWN2_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR8933333.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
BASE COUNT         1076 a          746 c          687 g         1123 t
ORIGIN      
        1 tgatatgctt tcattctttt tattgactat tctacagtat ggtctaacac catctaaatt
       61 catacctaca tgactccata tattgtttta taaacatcat agtaaagggt gttatatttt
      121 catatagtat gcaagcttct ttgtatacta tgatttaaaa aaacggaaca aaaatgcaat
      181 tatattctgg cattgtttaa tgcggtttgt cttttttatt cattatttca atgaaagaat
      241 ttcaactaca aagttaattg atcctaccta cataatatag acacagtacc taaagtaccg
      301 tggagatcat ccggggaagc cccgggtctg ataccagtgc aggtagaccg ctatcttgtt
      361 ccggtaatgt tctgccgttc tttacggcat cacctctagc agaaacatcg cgtaactgcg
      421 ccaagtatga tgaacttctc ttaccgctgc gatctggttc aacaatatgc agatggtaca
      481 caacctcttt aaccagccca tccggattat tcagagccgc ttccgcaatc cggaataaaa
      541 gtgctgtttt accgtgaacc ctctgaaaat cagcgagcag tgttttaacc agccgacgtt
      601 cagagcgtac tgacagacgg tggacaattt gaataaacag ttcaatcaga ccgtagatgc
      661 tctcccgttg gcgacatgct gggagcggtg gaacaccgct tcggcaacga gcttccggcg
      721 tctccagtga agtgactgac ggataatggt tcatgctacc gagctaatga aacacgccag
      781 ttcacccgga tgttgggact ttgttgaacc gaagaacacg gcggtgcgga gtccggagag
      841 taacagaata gcaaagagct tcgtgaaaac aataaagtgt gactacatca gtatcatgcc
      901 caaacctgac gggttaacgg cagcaaagaa ccttgcagag gcattcgagc attataacga
      961 atggcatccg catagtgcgc tggattatcg ctcgccacgg gaatatctgc ggcagcgggc
     1021 caatgataac aggtgtctgg aaatataggg gcaaagccac ccacgaacga tccagcatat
     1081 aatcaagaaa ttagatttca tttaacataa gatagattat gtacaccaag aggcgtttcc
     1141 ttaatttctg aatttacagg ctcttttgct ctctttgcta tgctgtggat ttggaaagat
     1201 agagtgctgg ctgattattt aggtattttc atcagcatgt ccctgatagc actgttccat
     1261 cagcgctttc aggcgataat agtagacctt gggtagcctc tgcccatgtg taacttgata
     1321 ccccgagaac agccacgatg atctaagcgg ttcttatttc tccggtctca gggtcggtaa
     1381 tgccaacagt aagtccacag taatcaacga agagtttttt gaaccgcccc ggaaatcctg
     1441 gagactaaac gccctgagaa agaggtaaac agaatgacta aaaatacacg tttttccccc
     1501 gaagtccgtc aacgggcagt tcgtatggtt ctggaaagtc agggcgaata tgactcacaa
     1561 tgggcggcaa tttttagcca tgaaaacgta atcaactcgg tgaaccaatt caaaaaatat
     1621 actttatacc tgcggaagtg agaaaaccaa tatattcaat acttgcacta tttttaatgg
     1681 tgccagatat attggtactt tgctcataaa gagatgtatc acctttttta ttagaaatgt
     1741 aatttctgga gccttctata taaaattttg cactgggggt gacatagtat ccaatattcg
     1801 cagcaacaga gcagtaattt tggtttttta tttcatcttt aaaaattgtc tcagtctgat
     1861 agtgtttatc tgtatcagat gaaagaaccc aaccactata tttcagttct gctccaaact
     1921 caaaattctc atggcggtaa ttagcagtta atccaatata aggtattttg aagagctgtt
     1981 tgtaacctat tgttctttca ccactaggat gtgccccttt tttattcctg ctccccccgt
     2041 tttcactata aatataactc cctcccattg cattaaaact gtaacgactc tcttggtaac
     2101 ctgctattag tccaagtcga taatccaaat tatttaataa ccaaccttta agattcagct
     2161 cgaattcgtt agcatcacgg agatgcgtat tgggatgcca gctctggtcg gtccatacct
     2221 gaggggtatt ggaattgttc cagtctttat caaccatgct tgctttctgg ttacctaaag
     2281 tagtccaacc ggaaactccg aaagacactt ttggaattag cttccaatcg atgccacctc
     2341 taacaatagt cgcattactg tatttccagt ccagttgact aatttttctc cccccttcct
     2401 tgggatgata aacgcgttct tttgttttgc cactcagaga tcctagacta atatccgtac
     2461 tgatgttgtc cggtatgaaa agtggatagt tagtggtagc atgtgtagta aagatcgcag
     2521 gtacacaaag tgcaagtaca aagaatttta atttcatcga gaactctact tttttggttg
     2581 aaatgtccat gatcttctta ctttataaga taaacgtcaa tgccaagcat tattttaatt
     2641 aaatatttgt ttatgttacc aagaacatat cttttattat aatatttatt gaacttttgt
     2701 aaaaataaaa aaatacctca ttgtgcgaat aaagtaacgg gggccataga tttctctacc
     2761 taccttttgg tgttcatacc tgaggctgat agtttcagga ttccgcgaca attctactca
     2821 ccttcttgtt ctctcgccaa tatgaaatca atcagaaaag cgttccataa gaacaacgat
     2881 aagacaataa attgtcatca cggctgcaat agccaacgtg cctttcagta ccaacatgac
     2941 aacctcctgt atttgacgta acgtgtgctg ataaatatgt atttgtatct caactgactc
     3001 aatacaacac ttaagtatag tttttcagat tttgtttttc ccgtgaaata ttatggaaat
     3061 ggcagaagca atacattgaa ttccgattcg agtgaatacg gctagcgggc acctcacttt
     3121 agcactgaag ccatcaactt ttatttgact tatttcaatg gaaagctttc tcactatcag
     3181 tacaggtaat tcccatcctt ttaaagtcga gagtcagact gcgactaata cagttaaagt
     3241 gaccaaactc agttatcctc tacacatttt atctcatgtt tacttggaca attcattggt
     3301 gcttttataa aagaataaat attctactaa tttaatgcat cacctaaaat agtatttttt
     3361 ataatagaac tgtatctata gtatattatt aaagcttaat tatttattat gctttaatgt
     3421 ggttgtccga ttaaggacta atccttgaaa acgccgtata gggcgagagt tcactctatt
     3481 gcgataacgt aaccgtacaa tattgtgcgt ttcccaaatt gaccactact gaactattct
     3541 gggatttaaa tgcaataaaa tggttggttg aaggtcgtgg tgatgatgtt agtccttttg
     3601 agaatattcc agtgccagat cttacttata at
//
LOCUS       DAZSNO010000258         3553 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZSNO010000258 DAZSNO010000000
VERSION     DAZSNO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.261"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..66
                     /locus_tag="ACHWN2_004044"
     CDS             <1..66
                     /locus_tag="ACHWN2_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFR8933334.1"
                     /translation="AELITGKLYDRIPKEIWKYVR"
     gene            105..695
                     /locus_tag="ACHWN2_004045"
     CDS             105..695
                     /locus_tag="ACHWN2_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR8933335.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            935..1195
                     /locus_tag="ACHWN2_004046"
     CDS             935..1195
                     /locus_tag="ACHWN2_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR8933336.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1419..1493
                     /gene="tap"
                     /locus_tag="ACHWN2_004047"
     CDS             1419..1493
                     /gene="tap"
                     /locus_tag="ACHWN2_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR8933337.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1486..2343
                     /gene="repA"
                     /locus_tag="ACHWN2_004048"
     CDS             1486..2343
                     /gene="repA"
                     /locus_tag="ACHWN2_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR8933338.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3044..3235
                     /locus_tag="ACHWN2_004049"
     CDS             3044..3235
                     /locus_tag="ACHWN2_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933339.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
     gene            complement(3465..>3553)
                     /locus_tag="ACHWN2_004050"
     CDS             complement(3465..>3553)
                     /locus_tag="ACHWN2_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933340.1"
                     /translation="RFTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT          945 a          894 c          890 g          824 t
ORIGIN      
        1 gcggaattaa tcaccggaaa gttatatgac aggataccaa aagagatatg gaaatatgta
       61 aggtaataat tcagcaataa ttaattgcca ggagcagata aataatgaag ttgattattt
      121 ttattctcat tgttctgatt attgcagcgc tacttatccg gattatcctc agaagtgtaa
      181 atcagcattc tcctttgctg atgcaattgc atgcggcagg tatacggact ggtgatgcag
      241 agcgaatact gtccagcggt gaatactggc aacgtcagaa gacgctgctg acagaaaggg
      301 aagtcagttt tatgaaagga ctgttcagaa ttgtggatat gaagcggtgg tatctgtgtc
      361 cgcaggtacg ggtagcggat atcgtccagc tgaacgggaa tatccggcca cgatcacgcc
      421 agtggtggca gttattcagg atggtgtctc agtggcatgt tgatgtggtt atcgttgagc
      481 tgcgttcgtt cagtattgtt gcagcagtag agctggatga tgccagccat ttacgaccgg
      541 aacgcagacg ccgggatatt cttctggaag aggttctgag gcaggctggt attccgttgc
      601 tcagaagcca cgatgccaga aaactgctgc agatgaccgg agaatggctg aatacaacag
      661 gggctgatca gcagtcaccg gaacatcgta gctgacgcct tcgcgttgct cagttgtcca
      721 accccggaaa cgggaaaaag caagttttcc tcgctcccgg cgtttccata actgaaaacc
      781 atactatttc acagtttaaa tcacattaaa cgacagtaat ccccgttgat ttgtgcgcca
      841 acacagatct tcgccacaat tctcaagtcg ctgatttcaa aaaactgtag tatcctctgc
      901 gaaacgatcc ctgtttgagt attgaggagg cgagatgtcg cagacagaaa atgcagtgac
      961 ttcctcattg agtcaaaagc ggtttgtgcg cagaggtaag cctatgactg actctgagaa
     1021 acaaatggcc gctgttgcaa gaaaacgtct tacacacaaa gagataaaag tttttgtcaa
     1081 aaatcctctg aaagatctca tggttgagta ctgcgagaga gaggggataa cacaggctca
     1141 gttcgttgag aaaatcatca aagatgaact gcagagactg gatatactaa agtaaagact
     1201 ttactttgtg gcgtagcatg ctagattact gatcgtttaa ggaattttat ggctggccac
     1261 gccataaggt ggcagggaac tggttctgat gtggatgtac aggagccaga aaagcaaaaa
     1321 ccccgataat cttctttaac tttggcgagt cagaaagatt accggggccc acttaaaccg
     1381 tatagccaac aatcaagcta tgcggggagt atagttatat gcccggaaaa gttcaagact
     1441 tctttctgtg ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa
     1501 caaacttatt accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgcc
     1561 cggacgcttc cgttctgcgg gaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt
     1621 gatttcgcca ttcatgtggc acatgcccgt tcgcttggtc tgcgtcggcg catgccaccg
     1681 gtactacgtc gccgggccat tgatgcgctg ctgcaggggc tgtgttttca ctatgaccca
     1741 ctggccaacc gcgtccagtg ttccatcacc acgctggcca ttgagtgcgg actggcgacg
     1801 gagtctgctg ccggaaaact ctccatcacc cgggccaccc gggccctgac gttccttgca
     1861 gagctgggac tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg
     1921 accgatatca cgttcacatc tgcactgttt gctgccctcg atgtatcaga ggaggcagtg
     1981 gccgccgcgc gccgcagtcg tgtggaatgg gaaaacagac agcgcaaaaa gcaggggctg
     2041 gataccctgg gtatggatga actgatggcg aaagcctggc gttttgttcg tgagcgtttc
     2101 cgcagttacc agacagagct taagtcccgt ggaatgaagc gtgcccgtgc gcgtcgtgat
     2161 gcggacaggc agcgtcagga tattgtcacc ctggtgaaac ggcaactgac gcgtgaaatc
     2221 tcggaagggc gcttcactgc cagtcgtgag gcggtaaaac gtgaagtgga gcgtcgtgtg
     2281 aaggagcgca tgattctgtc acgtaaccgt aattacagcc ggctggccac agcttccccc
     2341 tgaaagtgac ctcctcagaa taatccggca cgcgccggag gcattcgcac gcctgaagcc
     2401 cgccagtgca caaaaaatca gcgctacata caaaaaacaa cctcatcatc caccttcagg
     2461 tgcatccggt cccccctgtt tttgatacaa aacacgcctc acagacgggg aattttgctt
     2521 atccacatta aactgcaagg gacttcacca taaggttaca accgttcacg tcataaagcg
     2581 ccagacgtca accctacagg gtgcaatgta tcttttaaac acctgtttat atctccttta
     2641 aactacttaa ttacattcat ttaaaaagaa aacctgttca ctgcctgtcc tgtggacaga
     2701 caaatatgca cctcccaccg caagcggcgg gcccccaacc gaagccggtt tagttacaac
     2761 atgcaaacat aaacccctaa aaaacccaag tccaacgcaa aactgaaacc acaacgcccc
     2821 cctcataact gaaaagcgcc gccgcccccg cccaaagggc gggaacagag tcgcttttaa
     2881 ttatgaatgt tgtaactaca tcatcatcgc tgtcagtctt ctggctggac gtatcgaata
     2941 cacgctcgta agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa
     3001 aaccctcgtc gggaccactc cgaccgcgca cagaagctct atcatggctg aaaacgggta
     3061 tggcttggca gggctgggga tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc
     3121 gggcttcggc ggttttactc cggtatcaca tgtaaccaca gcgtgccgcc ttccatgccg
     3181 ctggcgcggc atcagaataa tacaagcagc atttaatgca tttgcaaaag tgtgagtaat
     3241 attaagtggt cgtttcaggg atatcgttaa gacttgagcg ataggaatta aaaccccaaa
     3301 aagattaaaa aaacaccaca aaacggatgt ttcttcaaca ccacttttgc tccatatgaa
     3361 cggaaccgac gattaaactg gatggctctg attgattcag ggtatgaatg gcggtttttt
     3421 gctccgtttc cctcaaaatg gacgcaactt cccctctgcg gctctcagcc gcaccaccgc
     3481 atccgggcca gcggctcatg catcaggacc tgctctgcca gacggtagcc ccgcttcagc
     3541 cccgtaaaac gca
//
LOCUS       DAZSNO010000259         3548 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZSNO010000259 DAZSNO010000000
VERSION     DAZSNO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.262"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACHWN2_004051"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACHWN2_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR8933341.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="ACHWN2_004052"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="ACHWN2_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR8933342.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHWN2_004053"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHWN2_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR8933343.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAZSNO010000260         3546 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZSNO010000260 DAZSNO010000000
VERSION     DAZSNO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.263"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..737)
                     /locus_tag="ACHWN2_004054"
     CDS             complement(<1..737)
                     /locus_tag="ACHWN2_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933344.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNDSQELNNSQKLNN
                     SQELNNSQVSCKGSVDSTITDLLEKAL"
     gene            complement(860..1312)
                     /gene="tabA"
                     /locus_tag="ACHWN2_004055"
     CDS             complement(860..1312)
                     /gene="tabA"
                     /locus_tag="ACHWN2_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR8933345.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1457..2050)
                     /locus_tag="ACHWN2_004056"
     CDS             complement(1457..2050)
                     /locus_tag="ACHWN2_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR8933346.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            2121..2834
                     /gene="bdcA"
                     /locus_tag="ACHWN2_004057"
     CDS             2121..2834
                     /gene="bdcA"
                     /locus_tag="ACHWN2_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR8933347.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2966..3361
                     /locus_tag="ACHWN2_004058"
     CDS             2966..3361
                     /locus_tag="ACHWN2_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR8933348.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
BASE COUNT          946 a          844 c          760 g          996 t
ORIGIN      
        1 ttcaatgctt tttctaataa atccgtaatc gtagaatcaa ctgaaccttt acaactcacc
       61 tgcgagttat ttaactcctg cgaattattt aacttctgtg aattatttaa ctcctgcgaa
      121 tcatttaact cctgcgaatc atttaactcc tgtatattat ttaacttctg atgattattt
      181 aatagacgcg ctattttctt gttagcttcg cgttggtaac acgccagaac cttgttagta
      241 atcaggtgta atgaactatc atcttttctt tcaaaagact tcccttcata acgattagtc
      301 gaaccatcag attgtttaaa tataatgact gtatgtcctt cacgaatccc tttagtagaa
      361 gctgaggcaa gtgtaatttc tatcttcacc ttagataaat ccgctcgctc cccttcagga
      421 ttattcatgt aaaacttcat tttttcattg ccatcagtat ctttagaata agatgagaac
      481 tcaatatgag ccatcatatt tctacccgct tcgttggagc gtttgctaaa atccacataa
      541 cccagggtaa atatctttgc caaaaactcc aaaaatgctc taggagaaag aatattgctt
      601 ctgctattct gccccccggc aattcgggca aaatcattca ttcccacata ccaggtatct
      661 aaggcttcat ttttttgttt aaaattgtcg gctaatgtaa tgtgttgaga ataattcaaa
      721 tctgatattt tgcccatgac gaactccttg agttaataag aacgtctaag cattacaaat
      781 tttactcaca aaaaaatatc aaaaaacgct cttttcacaa actcgccgta aacctggagt
      841 tatttacggc gaaaagtcat cacgccatca gcatcttcac tactgctttg cgaacctggg
      901 ctggcgcgcc cactgcgcac agcggtttat gcacttcccc cggataaaac acaacaaaat
      961 caccttcatt taggataact gttttctcat caacgccttc cggcaaaaat gcgatgtctt
     1021 tatcagctaa ccagtcggta tccggcgcgc ctgcaggttg cgtgctgaag gtcatgcctt
     1081 cctgaccttt taacacaatc tgaatgtcaa gatagcgggc atggtactcc gcacggcgag
     1141 cttcgtacgg ctcggtcata tcttccgaga taagataaaa cagacgattg ccttcgatat
     1201 catgcttgcc ctttggcgtt tctgccgtaa cgtgtgcttt gatatgctca atcgcctggc
     1261 gtaactcctg gggtagccac ggctgaagat tatgaatatt tccgatgatc atcttttcca
     1321 ctccttaatt aaaacaatgt ttcatttcta tcgttatacg aaaagatgat caattccgcc
     1381 agccttaacg cgtgaattat tgtcttatca caacttatcg gcaggcgcgt tcccgccttt
     1441 cggatcaagg ccagcatcac tccttgagaa gagtttttat cgcttcccct gccagggcag
     1501 ctgttgcaca gagttgttcg atggagtgcc cctcccgtgc cttcgcagaa agccctgaca
     1561 tcacggtact cataaaatca gtcacacatt gtgcgctttg tggatgccgc ctggcgatat
     1621 agtcataaat ggtcgtttct gcggcgtgat aatattgaat ggcaatatca cgcgcttgtg
     1681 gatcatgact atgaatacct tcaagaacca tacagccagc gcagccgccg ttttggctat
     1741 atctgcgcgc cgcttctttt aatacctcaa ccaggcactc gcctaccgga cgatcatcac
     1801 gaagaatatc ggcaagcgga atagcttccg taccgacata ttcattgagt acacggctaa
     1861 ataacccagc cttactgcca aaagccgcgt agagactcgg ggggttaata ccaagataat
     1921 cagtaacttc agagacactt actgcatcga aacctttttg gtgaaaaagc acttttgccg
     1981 cagagactgc ctgctcagga gcgaaacgtc tgggacgacc tggaacgcga gattgttttt
     2041 tagtgaccat tacaaaacct gttgacagaa agttaaaaca gttttgtaat gcatgttaca
     2101 taataaatca aggagtcctt atgggcgctt ttaccggtaa gacagttctc atcctcggtg
     2161 gcagtcgtgg aatcggtgcc gctatcgtac gtcgtttcgt caccgatggg gccaatgtac
     2221 gattcaccta tgcggggtcg aaagatgccg ctgaacgtct ggcacaagag actggagcga
     2281 cagcagtatt cacagatagt gctgacagag acgctgttat tgatgtcgtt cgtaagagcg
     2341 gcgcattgga tatcctggtg gtaaatgcag gtattggcgt ctttggcgat gccctggaat
     2401 taaatgccga cgatattgat cgccttttca aaatcaatat tcatgctcct tatcatgcct
     2461 ctgttgaagc cgcccggcag atgcccgaag gcgggcgcat cttaatcatc ggctccgtga
     2521 atggcgatcg tatgcctgtt gcaggcatgg ctgcttatgc cgccagtaaa tctgccctgc
     2581 aaggcatggc gcgcgggctg gcccgtgatt ttggaccgcg agggatcacc atcaacgtcg
     2641 tccagccagg accaattgat accgacgtta atcccgccaa cgggccaatg cgcgatatgt
     2701 tgcatagttt tatggccatc aaaagacatg ggcaaccgga agaggtcgct ggtatggtcg
     2761 catggttagc agggccagaa gccagttttg ttaccggcgc gatgcatacc attgatggcg
     2821 cgtttggcgc ataaccgact acgctcaatt aagcccagcc attatccatg atgtctgggt
     2881 tttgtttact aacgtcgtcc ccctaaaagc ggctcccgat aaatataaat cttctgttca
     2941 tgtcgtaaat cttgaggata accaaatggt agaaagaacc gctgttttcc ctgctggccg
     3001 acattcactg tatgctgagc atcgttattc tgcggctatt cgttccggcg atttgctgtt
     3061 tgtttccggg caagtaggaa gtcgagagga cggaacacca gaacccgatt ttcagcaaca
     3121 agtcagactg gcctttgata atttgcatgc gaccctggca gctgcgggat gcacttttga
     3181 cgatatcatt gatgttacga gctttcatac cgatccggaa aaacaatctg aagacatcat
     3241 gacggtgaaa aatgaaatat ttagcgcccc accttatcca aactggacgg cggtgggtgt
     3301 tacctggctg gcaggctttg attttgaaat taaagtgata gcgcgtatcc ctgagctata
     3361 agcaatagtg ttagccgttc gttttcaccc tccgccctat aagtcggatg aatggaataa
     3421 aatgcatttc tgattgcgtg aaaatgaaaa agaaaaaagc aggaaatgtc tgcaattatt
     3481 gataccgaac gacacttccc ctccagaatc atagaaaata aaaatgcata tattttgact
     3541 tttaat
//
LOCUS       DAZSNO010000261         3526 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZSNO010000261 DAZSNO010000000
VERSION     DAZSNO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.264"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1147
                     /gene="yiaY"
                     /locus_tag="ACHWN2_004059"
     CDS             <1..1147
                     /gene="yiaY"
                     /locus_tag="ACHWN2_004059"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFR8933349.1"
                     /translation="SSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTKLG
                     MAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHDCA
                     KGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNKMA
                     IVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALKAV
                     TMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLPHG
                     VCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKVDI
                     PAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            1312..2850
                     /gene="aldB"
                     /locus_tag="ACHWN2_004060"
     CDS             1312..2850
                     /gene="aldB"
                     /locus_tag="ACHWN2_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFR8933350.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3069..3212)
                     /locus_tag="ACHWN2_004061"
     CDS             complement(3069..3212)
                     /locus_tag="ACHWN2_004061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933351.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3395..>3526
                     /locus_tag="ACHWN2_004062"
     CDS             3395..>3526
                     /locus_tag="ACHWN2_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR8933352.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT          879 a          844 c          929 g          874 t
ORIGIN      
        1 atcttcaact ttctttattc cttctgtgaa tgtcatcggc gctgattcat tgactgatgc
       61 aatgaatatg atggcagatt atggatttac ccgtacctta attgtcactg acaatatgtt
      121 aacgaaatta ggtatggcgg gtgatgtgca aaaagcactg gaagaacgca atatttttag
      181 cgttatttat gatggcaccc aacctaaccc caccacggaa aacgtcgccg caggtttgaa
      241 attacttaaa gaaaataatt gcgatagcgt gatctcctta ggcggtggtt ctccacacga
      301 ctgcgcaaaa ggtattgcgc tggtggcagc caatggcggc gatattcgcg attacgaagg
      361 cgttgaccgc tctgcaaaac cgcagctgcc gatgatcgcc atcaatacca cggcgggtac
      421 ggcctctgaa atgacccgtt tctgcatcat cactgacgaa gcgcgtcata acaaaatggc
      481 gattgttgat aagcacgtga ctccgctgct ttctgtcaat gactcctctc tgatgattgg
      541 tatgccgaag tcactgaccg ccgcaacggg tatggatgcc ttaacgcacg ctatcgaagc
      601 gtatgtttct attgccgcca cgccgatcac tgacgcttgt gcactgaaag ccgtgaccat
      661 gattgccgaa aacctgccgt tagccgttga agatggcagt aatgcgaaag cgcgtgaagc
      721 aatggcttat gcccagttcc ttgccggtat ggcgttcaat aatgcttctc tgggttatgt
      781 tcatgcgatg gcgcaccagc tgggcggttt ctacaacctg ccacacggtg tatgtaacgc
      841 cgttctgctg ccgcatgttc aggtattcaa cagcaaagtc gccgctgcac gtctgcgtga
      901 ctgtgccgct gcaatgggcg tgaacgtgac aggtaaaaac gacgcggaag gtgctgaagc
      961 ctgcattaac gccatccgtg aactggcgaa gaaagtggat atcccggcag gcctacgcga
     1021 cctgaacgtg aaagaagaag atttcgcggt tctggcgact aatgccctga aagatgcctg
     1081 tggctttact aacccgatcc aggcaactca cgaagaaatt gtggtgattt atcgcgccgc
     1141 aatgtaatca tcattccaac aacggctggc aaattgtcag ccgctttttc aactatctct
     1201 gtaatccttg cccgtaaatt cgtgatagct gtcgtaaagc tgttaccgac tggcgaagat
     1261 ttcgccagtc acgtctaccc ttgttatacc tcacaccgca aggagacgat catgaccaat
     1321 aatccccctt cagcacagat taagcccggc gagtatggtt tccccatcaa gttaaaagcc
     1381 cgctacgaca actttattgg cggtgaatgg gtagcccctg ccgatggtga gtattaccag
     1441 aatctgacgc cggtgaccgg gcagctgctg tgcgaagtgg cgtcttcggg caaacgagac
     1501 atcgatctgg cgctggatgc tgcgcacaaa gtgaaagata aatgggcgca cacctcggtg
     1561 caggatcgcg cggcgattct gtttaagatt gccgatcgaa tggaacaaaa cctcgagctg
     1621 ttagcgacag ctgaaacctg ggataacggc aaacccattc gcgaaaccag tgctgcggat
     1681 gtaccgctgg cgattgacca tttccgctat ttcgcctcgt gtattcgggc gcaggaaggt
     1741 gggatcagtg aagttgatag cgaaaccgtg gcctatcatt tccatgaacc gttaggcgtg
     1801 gtggggcaga ttatcccgtg gaacttcccg ctgctgatgg cgagctggaa aatggctccc
     1861 gcgctggcgg cgggcaactg tgtggtgctg aaacccgcac gtcttacccc gctttctgta
     1921 ctgctgctaa tggaaattgt cggtgattta ctgccgccgg gcgtggtgaa cgtggtcaat
     1981 ggcgcaggtg gggaaattgg cgaatatctg gcgacctcga aacgcatcgc caaagtggcg
     2041 tttaccggct caacggaagt gggccaacaa attatgcaat acgcaacgca aaacattatt
     2101 ccggtgacgc tggagttggg cggtaagtcg ccaaatatct tctttgctga tgtgatggat
     2161 gaagaagatg cctttttcga taaagcgctg gaaggctttg cactgtttgc ctttaaccag
     2221 ggcgaagttt gcacctgtcc gagtcgtgcc ttagtgcagg agtcaatcta cgaacgcttt
     2281 atggaacgcg ccatccgccg tgtcgaaagc attcgcagcg gtaacccgct cgacagcgtg
     2341 acgcaaatgg gcgcgcaggt ttctcacggg caactggaaa ccatcctcaa ctacattgat
     2401 atcggtaaaa aagagggcgc tgacgtgctc acaggcgggc ggcgcaagct gctggaaggt
     2461 gaactgaaag acggctacta cctcgaaccg acgattctgt ttggtcagaa caatatgcgg
     2521 gtgttccagg aggagatttt tggcccggtg ctggcggtga ccaccttcaa aacgatggaa
     2581 gaagcgctgg agctggcgaa cgatacgcaa tatggcctgg gcgcgggcgt ctggagccgc
     2641 aacggtaatc tggcctataa gatggggcgc ggcatacagg ctgggcgcgt gtggaccaac
     2701 tgttatcacg cttacccggc acatgctgcg tttggtggct acaaacaatc aggtatcggt
     2761 cgcgaaaccc acaagatgat gctggagcat taccagcaaa ccaagtgcct gctggtgagc
     2821 tactcggata aaccgttggg gctgttctga tataagaagc tggtcgcatt gggtattcat
     2881 tgcctgatgc gacgcttacg cgtcttatca tgcctacggg aacctgaccg taggccggat
     2941 aaggcgtaca cgccgcatcc ggcataaaca aagcgcacgt tgttacaatt tacgttttaa
     3001 acctacgtgc gcaataaccc agactacgcc aacagaagtg cgtttctggg gcgtgttttg
     3061 tcaggagttt aatctcgcgt ttcgatggtg agcgggcgaa ccaggccgta aatcaccatt
     3121 ggtgatccga ggccaatcag caataaacgg tagataattg aaggtttaat acgcttaatc
     3181 tgtactgtgg atgttttact ggagttattc attagtttgg ccaatagttc cactatttca
     3241 atatgataaa cctcgtcctg ttgaaaaacc agacagcaca tttcagaaag tatataaaaa
     3301 acaatatgtc gttatataaa taaggaaatt gcactgcatc atggcatttc ataatatgcg
     3361 ttaattgttc ttttttatat tctttgaggg aaatatgttc ctggactatt ttgcactggg
     3421 agtgcttatt tttgtatttc tggtgatatt ctatgggatt attattttac atgatattcc
     3481 ctacctgatt gctaaaaaac gtaatcatcc tcatgccgac gccatt
//
LOCUS       DAZSNO010000262         3489 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZSNO010000262 DAZSNO010000000
VERSION     DAZSNO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.265"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(746..2350)
                     /locus_tag="ACHWN2_004063"
     CDS             complement(746..2350)
                     /locus_tag="ACHWN2_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8933353.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
     gene            complement(2465..2539)
                     /locus_tag="ACHWN2_004064"
     tRNA            complement(2465..2539)
                     /locus_tag="ACHWN2_004064"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2504..2506),aa:Gly,seq:tcc)
     gene            complement(2543..2618)
                     /locus_tag="ACHWN2_004065"
     tRNA            complement(2543..2618)
                     /locus_tag="ACHWN2_004065"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2583..2585),aa:Thr,seq:tgt)
     gene            complement(2621..2697)
                     /locus_tag="ACHWN2_004066"
     tRNA            complement(2621..2697)
                     /locus_tag="ACHWN2_004066"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2661..2663),aa:Arg,seq:tct)
     gene            complement(2699..2775)
                     /locus_tag="ACHWN2_004067"
     tRNA            complement(2699..2775)
                     /locus_tag="ACHWN2_004067"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2739..2741),aa:Ile,seq:cat)
     gene            complement(2984..>3489)
                     /locus_tag="ACHWN2_004068"
     CDS             complement(2984..>3489)
                     /locus_tag="ACHWN2_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR8933354.1"
                     /translation="PVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDL
                     KELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          911 a          837 c          771 g          970 t
ORIGIN      
        1 aagacctata ggtttggtga ttgattatta taaatatcat caccccttaa ggtattccct
       61 tagagttaat ctttaattgg tctttaatta aatcttttat ttaatactta aagttaatct
      121 ctaattgtgt ctttaagtta actttaagta atactataag ttaatcttta attgtgtctt
      181 taagttaact ttaagttaag cccctgtagg gactagagac cataattaag atgattgtgt
      241 cttattgcta ttcacttatc gactgaccca gagcacacag gaagcattca ctttaagtag
      301 aacgcttggt gtctgtaatt ggtcatcccg cccctgaaag gatgcgataa gttacggtta
      361 gttgtcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      421 tccttgacaa gtagagaaaa ccatgagtca aaaacatgac cacacaggtc tgtagtaagt
      481 gtcatcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      541 ccccttgaca agcagagaaa actatgagtc aaaaacatga ccgcacaggt ctgtagtaag
      601 tgtcatccta gaagagaaaa ctttttgtgt cccttctccc tatagtgagt cgtattaatt
      661 atcccttgac aagcagagaa aactatgagt caaaaacatg accacacagg tctgtagtaa
      721 gtgtcatcct agactatggt ctcctctagg tctccttata aacatcagcc atcacattga
      781 ccagcctatc aacctcgacc tctttagcat aacggtcgta cgtaatagac cctgaactat
      841 gccccatgat cgcctgtgca tacgccaact cgaccccatg ttgttttaac tgggccgcaa
      901 ctgtatgtct aaacgagtga aacgctaagc cgctacggtc tacgaggact ttaggcaaca
      961 acgtccgatt aaaccattta ctcacctgct caccgtaacc gttcttcatg agacgcagac
     1021 catcgaacac catagcgtca tcaccgtcag cgcctctacg ggtctctaca agcgccataa
     1081 agtcagccaa tataaaacca taggccccat cgaccagagg cacacagcgg tcactatgag
     1141 cgttcttgat gctcttaccg ggtagactac tatcgtcttc attgatatga atataaacgg
     1201 ttcccgcctc agtggtcctg acgtctttga cctgtagttg ggctatttca ttgagtctcg
     1261 ccccagtgat agcagccaag gcggtaacat aataatgata cggcttacca gaggtcttct
     1321 gagagtatgc cttagctgcg accagtagtt gccccacctg ctccgtagag aaggcgttac
     1381 gggcccctga ggctttacgc tgagggactt taagctccag accctcagtc atgttctttt
     1441 taatcaagtc attgcgcaca gcccacctaa agacagcagc catcttaata agatacttgt
     1501 tgttgatggt aacgacatcc agacaatctg tcttatcttc acggctcaac aggtcagcaa
     1561 gtgggacatc cttgaagcgc tgcttacggt tcctaggtaa ctgctggagc acatcacgga
     1621 ccctaagcat gtcagccctg ttagcgtctg cattgagacc cagatggtcg aatatttcga
     1681 ttaacgcagc gtgtgacgcc ttgttttccc ttaaggttgc aggcttccag ttctgatagt
     1741 tctccgcttc gtattgttca aagaggctcg ctatggtaat gctaggcgta acttctggtt
     1801 ttacttctgg agcattaaca gatagggaca cagagtctgc ttggttatct tgcctatcaa
     1861 tatcattaaa aagatcgacg taatctatca gaggctgact gtctcccgct tcgatacgcc
     1921 tcatacaggc ttttagcacc ttcagggcct cattgatgta acgatgctgg tcaatgctta
     1981 gcggctcact agccagagca tcggtaaggc tctcaccaag ttctccgtat tggtcccggt
     2041 agatatccct catgtcaggc tcaaagagat ctgaacggcc catagacagt tcacactcag
     2101 cgatgtcctt aaggtgttcc ctcatttcct cataggtagc gttgggtctg tctgcgtgta
     2161 ttgccttgag tgctgccttt atgtgccttg agtaagccat agcggtgcgt ctatccttgg
     2221 tccgtaatga tattgaggtc attctatcat tgtttgattg tctgacccta agacggaagt
     2281 agtagacgcc gttacgctgg tagagatagt agactgctgg aagtttgtga ctactggata
     2341 gctgaatcat atgtctgcat gccctgagag ggttacacag tgggttacag taagtaactc
     2401 tgcaaggagg cttaaagccg tatcccttag gattcagtag gttggagtac tttaagaaag
     2461 gtcatggagc gggcagcggg aatcgaaccc gcatcatcag cttggaaggc tgaggtaata
     2521 gccattatac gatgcccgca tatggtgccg actaccggaa tcgaactggt gacctactga
     2581 ttacaagtca gttgctctgc ctgctgagct aagtcggcgc tggcccacca ccgaggactc
     2641 gaacctcgca ccgtcaactt agaaggttga tgctctatcc ggatgagcta gtggtggttg
     2701 gtggcccttg ctggacttga accagcgacc tggcgattat gagtcgctcg ctctgaccaa
     2761 ctgagctaaa gggccggagg cagaataata accatatgtc atcacatctg caaactcatc
     2821 tgaccaccag cgcgtttaac gccctgtgcc atttttcagg cacaaaaaaa cccgcataaa
     2881 gcgggttctt tcaagtgtcc atgtctgcta ttcgcctcgc gatacagctt tgcgaagctt
     2941 accgtaattg aagcagttta tgtgcaaaaa tgcaagaact tttttaaagc tgcatcaacc
     3001 tttccaccag tttatctctg cgaacaacaa accaaccatt ggctctcgcc agttccagcc
     3061 atgactcaag ggaaataaca atatcatcat cccgtaactg aattgtggaa acagtgacac
     3121 cgcctctctg ataacagaga actcgcgtgt cgtaactttt ctggcatgaa actggcgttg
     3181 acggatccct ttgactgaaa tagcagtctt ccagtttttc gaacacatcc cacgcctgat
     3241 cggtttcgag cattttgccg tggcgtgctg cgcctcgttc tgtccagagg atgagggagc
     3301 gggctttggg tgaaactgga ttttgtgagt agtttaaagc gacccgcaat tctttaaggt
     3361 cattaccaac aactttgaaa aagtgtttcc cttcaacgaa gcgtactttg ttctcatgat
     3421 gattctggcg aatacgcacc ggctcagtgc cgtaaagctg cgccaaaagt tcggtggtaa
     3481 taacaggaa
//
LOCUS       DAZSNO010000263         3464 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZSNO010000263 DAZSNO010000000
VERSION     DAZSNO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.266"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            126..842
                     /gene="nanC"
                     /locus_tag="ACHWN2_004069"
     CDS             126..842
                     /gene="nanC"
                     /locus_tag="ACHWN2_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR8933355.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            862..1968
                     /gene="nanM"
                     /locus_tag="ACHWN2_004070"
     CDS             862..1968
                     /gene="nanM"
                     /locus_tag="ACHWN2_004070"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR8933356.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGEIAGGKAVTDSVLISVKDNKVTVQN"
     gene            2033..3013
                     /locus_tag="ACHWN2_004071"
     CDS             2033..3013
                     /locus_tag="ACHWN2_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR8933357.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3123..3328
                     /locus_tag="ACHWN2_004072"
                     /pseudo
     CDS             3123..3328
                     /locus_tag="ACHWN2_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1018 a          735 c          808 g          903 t
ORIGIN      
        1 ataaaataca gttatccgtt gccaaagaca ctataagcct ggcaaaaaaa tattacacaa
       61 cataaatgct aattgtttat gcgggctttg tattgctttc tgtatcctac aaatgagtga
      121 aatttatgaa aaaggctaaa atactttctg gcgtattatt actgtgcttt tcgtccccat
      181 taatttctca ggctgcgaca ctggacgtac gtggtggata tcgtagtgga agccacgcct
      241 atgagactcg actcaaagtc agtgagggat ggcaaaatgg atggtgggca agcatggaaa
      301 gtaatacctg gaataccatt catgataata aaaaggaaaa tgccgcactc aatgatgttc
      361 aggttgaagt taattacgca attaaacttg atgatcaatg gacggtgcgc ccgggaatgt
      421 taacgcattt tagcagcaac ggcacacgct acggacccta cgtaaaactg tcctgggacg
      481 cgacaaaaga tcttaatttt ggcattcgct atcgttacga ctggaaagct taccgacaac
      541 aagacttatc cggtgatatg tctcgtgata acgttcatcg ttgggatgga tatgtcactt
      601 accatattaa tagtgatttc accttcgcat ggcaaacgac gctatacagc aaacagaatg
      661 attatcgcta tgcaaaccac aagaaatggg cgacggaaaa tgcctttgtt ctacaatacc
      721 atatgacgcc cgatattacg ccatacatag aatatgacta ccttgaccgc cagggtgttt
      781 acaacggcag agataattta tcggaaaaca gttatcgcat tggtgtgtca tttaaactgt
      841 agtagacagg agacagtcac aatgaataaa actataatgg cgcttgctat catgatggct
      901 tcatttgccg caaacgcgtc tgtattaccg gaaactcctg tgccatttaa aagtggtacc
      961 ggagcaattg ataacgacac tgtctacatt ggtttaggta gcgcaggtac agcatggtac
     1021 aagctagata cacaggccaa agataaaaaa tggactgcgt tagctgcatt ccctggtgga
     1081 ccgagagagc aagcaacctc tgcatttatt gatggcaatc tgtatgtgtt tggcggcatt
     1141 ggcaaaaaca gcgagggatt gactcaggta tttaatgacg tacacaaata caaccccaaa
     1201 accaatagct gggttaaatt gatgtcgcac gcgccgatgg gcatggcggg tcatgtgact
     1261 tttgtacaca acggcaaggc ttatgttact ggcggtgtta accagaatat cttcaatggc
     1321 tattttgaag atctcaacga ggctggaaaa gattcaaccg ctatagataa aatcaacgcc
     1381 cactattttg acaaaaaagc agaagattat ttcttcaata agtttctgtt gtcttttgat
     1441 ccctcaacac agcaatggag ttacgctggc gaatcgccct ggtacggaac ggctggtgcg
     1501 gcggttgtga ataaaggtga taaaacctgg cttattaatg gcgaagccaa accaggattg
     1561 cgaacggatg ccgtatttga acttgatttc accggtaata atttaaaatg gaataagctt
     1621 gatcccgtct catcaccaga tggcgtcgct ggcggttttg cggggataag caatgattct
     1681 cttatatttg ctggaggggc cggattcaaa ggttcacgag aaaattacca gaacggtaag
     1741 aactatgcgc atgaaggcct gaaaaaatca tatagcactg atattcatct ttggcataac
     1801 gggaaatggg ataaatcggg tgaattatcg caaggtcggg cctacggagt atcattgccc
     1861 tggaataata gtttattaat tattggcggt gaaattgcag gcggcaaagc ggtgacggat
     1921 tcagttttaa tttctgtgaa ggataataaa gtcacagtac aaaactaacg cttcagggcc
     1981 ccggtaaagg ggccctgcta tcaatttgtc atttgtatta cgcgaaaatt atatgaacgc
     2041 aataatatca cccgattatt actatgttct taccgttgct ggtcagtcta atgccatggc
     2101 gtatggcgaa ggactgccat taccggacag ggaagatgcg cctcattcca gaattaaaca
     2161 attagcgaga tttgcgcata cgcatcccgg aggcccgcca tgtcacttta acgacattat
     2221 tccactgact cactgcccac acgatgttca ggatatgcag ggttatcacc atcctctggc
     2281 aacgaatcat caaacacagt acggcaccgt tggccaggca ctgcatattg cacggaaatt
     2341 actgcccttc attcctgata atgcagggat tcttatcgtt ccgtgttgcc gtggcggatc
     2401 ggcttttacc gcgggcagcg aagggacata ttcagaacgg cacggagcca gccatgatgc
     2461 ttgtcgttgg ggaacggata ctccgctgta ccaggattta gtcagcagaa cgcgagccgc
     2521 actggcaaaa aatccgcaga acaaattcct cggcgtatgc tggatgcaag gcgaatttga
     2581 cttaatgacc agtgactacg cgtcacaccc tcaacacttt aatcatatgg ttgaagcctt
     2641 tcgtagggat ctaaaacaat accattctca gcttaataat attactgacg cgccgtggtt
     2701 ttgcggcgat accacctggt actggaaaga aaatttcccg cattcgtatg aagctattta
     2761 tggcaattat caaaataatg ttttagccaa tattattttc gtcgacttcc agcaacaagg
     2821 tgaaagagga ctgacgaacg cgcctgatga agacccggac gatttaagca cgggatatta
     2881 cggttcagcg taccggtcac cggagaactg gacgacggca ctgcgaagca gtcatttcag
     2941 cacggcagcc cgtcggggga ttatttctga cagatttgta gaagcaattt tgcagttttg
     3001 gcgcgaaagg tgagcgggga gtttattcat gggggatgtc cttgttcgtt ctcagagtag
     3061 acaacaaaaa acaaatgaga tcaaggtaat atctcttcac ttgcgggctc gaacagcagg
     3121 caattgccaa tataataatc tggcacaagg gtgaaaaacg cgcaccgcat aaaccgttgt
     3181 tattgcttac agattagccg gatgccttaa tagacatccg cgccttttcg attatggctc
     3241 ggaaatctac gaacatctac acagtttact ggaacgtttt gggccacagc gttcgcagca
     3301 ccgtcctgat atgccgttct ggcgattata agtcgacgga ttctggcaaa tagacgatga
     3361 tgatatgctt aatgatgttc aaagaaatta ggccattatt atattattgc ttgcattttc
     3421 tttcattcct aaaagcaccc gccattaatc ccaggcgcat tatg
//
LOCUS       DAZSNO010000264         3464 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZSNO010000264 DAZSNO010000000
VERSION     DAZSNO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.267"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            339..1397
                     /gene="rfaQ"
                     /locus_tag="ACHWN2_004073"
     CDS             339..1397
                     /gene="rfaQ"
                     /locus_tag="ACHWN2_004073"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR8933358.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1394..2518
                     /locus_tag="ACHWN2_004074"
     CDS             1394..2518
                     /locus_tag="ACHWN2_004074"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR8933359.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2511..3308
                     /gene="rfaP"
                     /locus_tag="ACHWN2_004075"
     CDS             2511..3308
                     /gene="rfaP"
                     /locus_tag="ACHWN2_004075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR8933360.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          919 a          747 c          836 g          962 t
ORIGIN      
        1 tttgacttaa atagcaattt acccgccaat tcttgttgaa catctatact ttttttgtat
       61 ttttatgcga ttcattgatt tctgctgtac gtgcaggaag gaatcgcaga gcaacagtga
      121 aagaatatgg ttgctaatga ttttatttga ttgatttctt tattgaaaat caacaatgtg
      181 cttaaaaatc attcgggtcg gattgccgcg aaagaagtga tacactagcg cgtcaatatc
      241 agtgcggtat cagtattcag gtagctgttg agcctggggc ggtagcgtgc tttttctgct
      301 tcaccttaat cggataatct caacaaaaag agttacttgt ggataagcca tttcgaagaa
      361 ttttgctcat taagatgcgt tttcatgggg atatgttatt aactactccc gtcattagtt
      421 ctctgaaaaa aaattaccct gacgcaaaaa tcgatgtgct gctttatcag gacaccatcc
      481 cgatcctgtc tgaaaatcca gagattaacg cgctctacgg cataaaaaat aaaaaagcaa
      541 aagcctcaga aaaaattgcc aacttttttc atctcatcaa ggtattacgt gccaataagt
      601 atgaccttat cgtcaatctt accgatcaat ggatggttgc tatactggtt cgcttattaa
      661 atgctcgtgt gaaaatttcc caggattatc atcatcggca gtctgctttt tggcgtaaaa
      721 gtttcaccca tttggtgccg ttgcagggtg gaaatgtggt ggaaagtaac ttatccgtgc
      781 tgacaccatt gggacttgat tcgttggtga agcagacaac catgagttac ccgcctgcaa
      841 gctggaaacg tatgcgtcgc gaacttgatc acgctggtgt tggacaaaat tatgtggtta
      901 tccaacctac ggcgcggcaa atcttcaaat gctgggacaa cgccaagttt tccgctgtga
      961 ttgatgcctt acatgctcgt ggttatgaag tcgttctgac gtccggccca gataaagacg
     1021 atctggcctg cgtcaatgaa attgcgcagg gatgccagac gccaccagta acggcgctgg
     1081 ctggaaaggt gaccttcccg gaacttggtg cgttaatcga tcatgcgcag ctgtttattg
     1141 gcgttgattc cgcaccggcg catattgccg ctgcagttaa tacgccgctg atatcgctgt
     1201 ttggcgcaac agatcatatt ttctggcgtc cctggtcaaa taacatgatt caattctggg
     1261 cgggagatta ccgggaaatg ccaacgcgcg atcagcgtga ccgaaatgag atgtatcttt
     1321 cggttattcc ggcggcagat gtcattgctg ctgtcgataa attactgccc tcctccacga
     1381 caggtacgtc gttatgatcg ttgctttttg tttatataaa tattttccct ttggcggttt
     1441 gcagcgcgat tttatgcgta ttgctcagac agtcgccgcc cgaggtcatc atgttcgggt
     1501 ttatacccag tcgtgggaag gcgaatgccc tgatgtattt gaagtgatca aagtgccggt
     1561 taaatcgcat accaatcacg ggcgcaatgc ggagtatttt gcctgggtgc aaaaacattt
     1621 acgcgaacat cccgtcgata aagtcgttgg attcaacaaa atgccggggc tggacgttta
     1681 ttatgccgct gatgtttgtt atgccgagaa agtagcgcag gaaaaaggct ttttctatcg
     1741 cctgacgtca cgttatcgcc attatgccgc ctttgagcgg gcaaccttcg aacagggcaa
     1801 gccgacacag ctgctgatgc tgacagataa gcaaatcgcc gatttccaga aacattatca
     1861 gactgaagcg gagcgttttc atattctgcc accggggatt tatcctgatc gtaaatatag
     1921 ccagcagcca gccaatagcc gtgaaatctt ccgtaagaag aatggaataa ccgaacaaca
     1981 atatttattg ttgcaggtcg gttcagactt cacgcgtaaa ggtgccgatc gttccattga
     2041 agcacttgct tcgttaccgg attcgctgcg ccacaacaca ttgctatatg ttgttgggca
     2101 ggataaaccg cgaaaatttg aggcactggc agaaaaacgc ggcgtgcgca gtaatgttca
     2161 cttcttctcg gggcgcaacg atgtctcgga attaatggcg gcggcggatt tattactgca
     2221 tcctgcctac caggaagcgg cgggaattgt gctgctggaa gcgattactg caggattacc
     2281 ggtactaaca actgccgttt gtggctatgc gcattatatt gtcgacgcta attgcggcga
     2341 ggctattgct gagccattcc gccaggaaac attgaatgag attttacgca aagcgttaac
     2401 gcaatcttca ttgcgccagg cttgggcgga aaatgcgcga cattatgctg atacacaaga
     2461 tttatacagt ctgccagaga aagcggcgga tatcataacg ggtggtctgg atggttgaac
     2521 ttaaagagcc gtttgccacg ttatggcgcg gcaaagatcc ttttgaggaa gttaaaacct
     2581 tgcagggtga ggtatttcgt gaactggaaa ctcgccgtac tctgcgcttt gaaatggcgg
     2641 gaaaaagcta ttttctcaaa tggcatcgcg gcacgaccct gaaagagata atcaaaaatt
     2701 tactctcatt gcggatgcca gtattaggcg cagaccgcga atggaatgcg attcatcgac
     2761 tgcgggatgt cggcgttgat actatgtatg gggtggcatt cggcgaaaaa ggcatgaatc
     2821 cgctgaccag aacctcgttt attattaccg aagatctgac accaaccatc agtctggaag
     2881 attactgtgc tgactgggcg accaacccac cagatgttcg cgtaaagcgt atgcttatta
     2941 agcgtgtcgc gacgatggtg cgcgatatgc atgctgcggg cattaaccat cgtgactgtt
     3001 atatctgtca tttcctgctg cacttgcctt tttccggtaa ggaagaggag ttaaaaattt
     3061 cggtaattga cctacaccgg gcgcagcttc gcacgcgcgt tccaggtcgt tggcgcgata
     3121 aagatcttat agggctttat ttttcttcaa tgaatattgg cctgactcag cgggatatct
     3181 ggcgctttat gaaggtgtat tttgccgccc cgcttaaaga cattctcaag caggaacaag
     3241 gactgctgtc gcaagcagaa gcaaaagcca caaaaatcag ggaaagaacg attcgaaaat
     3301 cgttgtaacc cgtaacttat ttttgccaaa attttggata cagaataaat atgtctcaac
     3361 tcaatgatag tgacatcatc ctttttgagt ataattttca ttatcaaaat ataagatcta
     3421 aaaatactct tgatatagca tttggtattg acagaaattt tctt
//
LOCUS       DAZSNO010000265         3416 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZSNO010000265 DAZSNO010000000
VERSION     DAZSNO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.268"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..238
                     /locus_tag="ACHWN2_004076"
     CDS             <1..238
                     /locus_tag="ACHWN2_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFR8933361.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="ACHWN2_004077"
     CDS             248..886
                     /locus_tag="ACHWN2_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR8933362.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="ACHWN2_004078"
     CDS             889..2052
                     /locus_tag="ACHWN2_004078"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR8933363.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3416
                     /locus_tag="ACHWN2_004079"
     CDS             2136..>3416
                     /locus_tag="ACHWN2_004079"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR8933364.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGS"
BASE COUNT          760 a          805 c          987 g          864 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggttcc
//
LOCUS       DAZSNO010000266         3414 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZSNO010000266 DAZSNO010000000
VERSION     DAZSNO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.269"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(280..483)
                     /locus_tag="ACHWN2_004080"
     CDS             complement(280..483)
                     /locus_tag="ACHWN2_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933365.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(485..640)
                     /locus_tag="ACHWN2_004081"
     CDS             complement(485..640)
                     /locus_tag="ACHWN2_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR8933366.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(843..1250)
                     /locus_tag="ACHWN2_004082"
     CDS             complement(843..1250)
                     /locus_tag="ACHWN2_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8933367.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1327..1554
                     /locus_tag="ACHWN2_004083"
     CDS             1327..1554
                     /locus_tag="ACHWN2_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR8933368.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1538..1960
                     /locus_tag="ACHWN2_004084"
     CDS             1538..1960
                     /locus_tag="ACHWN2_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR8933369.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            2038..2815
                     /locus_tag="ACHWN2_004085"
                     /pseudo
     CDS             2038..2815
                     /locus_tag="ACHWN2_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2822..3414
                     /locus_tag="ACHWN2_004086"
                     /pseudo
     CDS             2822..3414
                     /locus_tag="ACHWN2_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          882 a          752 c          919 g          861 t
ORIGIN      
        1 gtaatgggtt aagtgataac agatgtctgg aaatataggg gcaaatccac tgagccagag
       61 gattacgaag ggcttcataa atatatgctc tcactaggat tcaggagaac gatcccccat
      121 ggagatggtt cgtataatca actcccggac ggaacttatg tttccgaaaa gggcggtgat
      181 atttatcaaa ttcgcagcca gatatctgac tatgcagacc gactatccgg gtatcgcgcg
      241 tctgtttttg tttgcgaatt cagtcaatgc gcatggtatt tataccccgc caagacccgg
      301 tgaatatcct gcacggcctt ctgctttgta gaaggcttct tcgctatcat aatcgccaga
      361 ttccagcgct tctttagcca gccagatgcg tgccccagtg cctgcatttg gctccagttg
      421 ctggaggcgt tttgcatctt ctaggagtag agcgataacg tgttttaatt ctgtctcgtt
      481 cattttactc acctgaatgt cttcccaacc aacgacgtgc gccagcttcg gttttaaacg
      541 ttttgctttt ggtatacgtt atggcggtga atgtgccgtc ctggttgggg aacacgccgt
      601 acaccggaga ttcgttgttg ccaagatcga tagtattcat gttgacccca tttcccctta
      661 acgccggggt agcggaactg tttgctgaga acaccgtgcg gtgtcttgat gaataaaatt
      721 tagaataacc taaggagggt gatcaagatt tttgtgtaga aaaacctaag ttttttgatg
      781 taaaaaacac aagtgtttga aagtttgtgc tttttattac agggtgtgga gaaaaaaggg
      841 gattatttgt ttgcgcttct tttgcgagct ttgagtagtt cttcaaaaag tttgttgaaa
      901 ttttcaactc gagcacgcat ctctgacaac agggcctttt gctctgactc aggcagtgcg
      961 tcgaacagtt gaagtaactc tttttgatct tctgtcagaa tggctggctg attatccggg
     1021 atcggttcgc ctggttgttt atcttcatcc ccaaaaagaa gccaagtcgg tgagcactga
     1081 agcgcttggc tcagtgcgaa taatcttttc cccgccggct gtgtttcatc tctttcccat
     1141 tgagaaattg ttacgtgagc cactttgacc agcttaccta atgcggcctg agacagtttt
     1201 aattttttac gcctatgtaa gaggcgagca ccgaaggttt cgtttttcat attagggaat
     1261 tctaattttt cttgacttag gtttctctac ggcctggttt ccttaggaaa atctaaggag
     1321 ctcgatatgt tgaaaattga tgctatagcg ttttttggca gcaaaacaaa gcttgccaat
     1381 gccgcaggag ttaggctggc aagcattgct gcatgggggg aactggttcc tgaaggtcgc
     1441 gcgatgcgcc tgcaagaggc atctggcggg gaacttcagt acgaccccaa agtttatgac
     1501 gaatatcgta aggcaaaacg acctgggaag gtgattcatg aaaatcaggc atgagcacat
     1561 cgaatcagtg ctgttagccc tggcagccga aaaagggcag gcgtgggtcg ctaacgcaat
     1621 tactgaagaa tatctgcggc aggggggcgg cgaattgtct ctggtaccag gcaaggactg
     1681 gaacaatcag cagaatatct atcaccgttg gttgaaaggt gaaacgaaag cgcaaaggga
     1741 aaaaattcag aaactgatcc ctgcggttct ggcaattctt ccgcgtgagc tgcgtcaccg
     1801 actctgtatc ttcgataccc tggaacgccg tgcattactg gcggcgcagg aagcgttgag
     1861 tacggcaatt gatgcgcatg atgatgctgt ccaggccgtt taccggaaag cacatttcag
     1921 cggtggtggg tcgcccggcg attctgtcgt agtgcattga ttgaaattaa tcgtgccgga
     1981 ttgttttgtt cggtatcagt taaatgtaac gctgcgagcg ttacaaggtg aaaacaaatg
     2041 gcttcaaact ggataaagct cgaggttatt acgccggata agccggaaat attcaggctt
     2101 gctgagattc tgaatattga tccagatgcc gcattaggga aggttattcg cttctgggca
     2161 tgggcggatc aacaaatgat agacggtaat gcagattgta acgctcgcgg cgttacaaaa
     2221 agtgcaatag atcgcatcac ttttatgtct ggttttgctg atgcgttaat tcaggttgga
     2281 tggctggtcg aaaatgacgg tgggctttct ctacctaact ttgaacgtca taacggaaaa
     2341 agctctaaaa aacgggcggt tacaaacgag cgagtaacaa aaatacgcga actgaaacga
     2401 aaaggtaacg ctgccagcgt tacacaaacg gatcaaaaag cgttaccagt ggaagaggaa
     2461 gaggaagatc taaatactga tctcccccta aatccccctc gccaaaaacg agcgtctaaa
     2521 aaattcgagc cggaggctat tgagctgccc gattggttgc cggaaacact ctggcatgag
     2581 tgggtccggt tcagacaggc attgcgaaaa ccgattcgaa cggagcaggg cgatacggga
     2641 actggaaaaa ttccgtcagc agggttttac acctgagcag gtgattcgac acagcatcgc
     2701 caatgaatac cagggcctgt tcgcgccgaa aggtgttcgg cctgagacgt tgctccgaca
     2761 ggttaacacc gtctcgttgc cggacagtgc gatcccgcca ggcttcaggg ggtaacggac
     2821 catgaaaaat attgcgacag gcggcgttct ggaacgcatc cgccgactga ccccgcaaca
     2881 tgtaaccgcc ccattcagaa cggtagcgga gtggcgcgag tggcaacttg ctgaaggcca
     2941 gaaacgtagc gaggagatca accgcctgaa tcgccagttg cgggtggaaa aaattctgaa
     3001 tcgctcaggc atccagccgt tgcaccgtaa atgctcgttt gcgaattacc aggtgcagaa
     3061 cgacggccag cgatacgcgt taagccaggc gaaatccatc gccgatgaac tgatgaccgg
     3121 gtgtacaaat tttgcgttca gcggaaaacc tgataccggg tagaatcact tagcggcagc
     3181 tatcgggaat cgcctgctga aagacggtca gacagtgatt gtggttaccg tggctgatgt
     3241 tatgagcgcc ctgcacgcca gctatgacga cgggcagtca ggcgaaaaat ttttgcggga
     3301 gctgtgcgaa gtggatctgc tggttcttga tgaaattggc attcagcgcg agacgaaaaa
     3361 cgagcaggtg gtgctgcacc agattgttga tcgccggaca gcgtcgatgc gcag
//
LOCUS       DAZSNO010000267         3383 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZSNO010000267 DAZSNO010000000
VERSION     DAZSNO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.270"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            19..897
                     /locus_tag="ACHWN2_004087"
     CDS             19..897
                     /locus_tag="ACHWN2_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR8933370.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="ACHWN2_004088"
     CDS             894..2285
                     /locus_tag="ACHWN2_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR8933371.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="ACHWN2_004089"
     CDS             2282..2539
                     /locus_tag="ACHWN2_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933372.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="ACHWN2_004090"
     CDS             2634..2834
                     /locus_tag="ACHWN2_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFR8933373.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="ACHWN2_004091"
     CDS             2827..3207
                     /locus_tag="ACHWN2_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR8933374.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          893 a          730 c          982 g          778 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaacac gctggtggtc
     3361 agatgagttt gcagatgtga tga
//
LOCUS       DAZSNO010000268         3352 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZSNO010000268 DAZSNO010000000
VERSION     DAZSNO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.271"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..149)
                     /locus_tag="ACHWN2_004092"
     CDS             complement(<1..149)
                     /locus_tag="ACHWN2_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HFR8933375.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
     gene            complement(255..1271)
                     /gene="phnD"
                     /locus_tag="ACHWN2_004093"
     CDS             complement(255..1271)
                     /gene="phnD"
                     /locus_tag="ACHWN2_004093"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8933376.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(1296..2084)
                     /gene="phnC"
                     /locus_tag="ACHWN2_004094"
     CDS             complement(1296..2084)
                     /gene="phnC"
                     /locus_tag="ACHWN2_004094"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR8933377.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(2217..2660)
                     /gene="yjdN"
                     /locus_tag="ACHWN2_004095"
     CDS             complement(2217..2660)
                     /gene="yjdN"
                     /locus_tag="ACHWN2_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFR8933378.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(2818..3153)
                     /locus_tag="ACHWN2_004096"
     CDS             complement(2818..3153)
                     /locus_tag="ACHWN2_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR8933379.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT          712 a          854 c          934 g          852 t
ORIGIN      
        1 aaggttgcca tgttgccgcc gtctttaatc agcgtaagcg gggccatttc cgcgccctgc
       61 cacgagacga ccagcacgtc gagaacaacg gcccagctca gaagcgagaa ccagctacgc
      121 ttgggtgggg cgatggtgat ggtttgcatg tttggctccg gtctgtaggc cggataaggt
      181 gcttgcaccg catccggcat caacgcctac acattgtcgg atgcgacgcc ggggcgtctt
      241 atccgaccta cgatttactg caccgcttta ctcaccgaac tcatcgcgct taacgcgttg
      301 ttcaggcggt ccaggtcatc cagctgcgcc tgaatctcgg tggttttcgc cagcttgtcc
      361 tgctcattca gccctttatt gcctttcacg ctttgtatct ctttaaacag tgcgagctgg
      421 cgaatcggca ccagttgcag gtcgctggaa gcgcggaacg gcgcccagcc caggcgttcc
      481 agcaccgctt tctcttccgg cgtttttccg tagttcataa agaagtcgta gatcttgtct
      541 ttggtagttt cggacagatt cttgcgccag acgatcggat cgcctaggat cagcggcgat
      601 ttccagatca ccttcagctc tttaagcttc tccggcgcgg aggttttcag cttgtcgagg
      661 ttttcggtgt tgttggtggc aacatccact tgcttgttgg cgacggccag cgcgttggtt
      721 tcatgcccgg cgttgacggt gcgcttgaag tcgctggcgg agatattgtt tttagcgaag
      781 acgtagtaac cggggacgag gaagccagag gtggagttag gatcgccatt gccgaaggtg
      841 agatctttcc gcttcgccag cagatcgttc aggttgttga tcgggctgtc tttgttgacg
      901 atcaacacgc tccagtaacc cggcgatcca tccgccgcga ccgtctgggc gaagacctgg
      961 ccattggcgc gatccaccgc ttccatcgcc gacaggttgc cgtaccaggc aatatccact
     1021 ttattgaagc gcatcccctg gataatgccc gcgtagtccg gggcaaagaa ggcgttgacc
     1081 ttcacgccca gtttcttctc catatcctgt aaaaacggcg tccattgcgg tttcaggttt
     1141 tgctgtgatt ccgttgaaat aatgccgaaa ttcagcgcct tttcctgctc ttcggcgtgt
     1201 gccggggtta acagggtgct gaggctgaac atgctggtga aggccagcga ggcaattatc
     1261 ttagcgttca ttcgttttcc tcaatgatgg ggatatcagg cagctttcgc gttctcttcg
     1321 atgcggttaa tgctgcggta gagatggtca aaacgttcgt tatcaaacag ttggctgctg
     1381 ccgtcgtaga agacgtgccc ctgacgcagg gcgacgatgc gttcgcagta gcgcagggcg
     1441 taatccacct gatgcagcgt gacgaccacg gtgatgccgt cgttctggtt gatgtcgcgc
     1501 agggtgtcca tcacgatgcg cgctgattcc gggtccagcg aggcgatggg ttcatcggcc
     1561 agaatcactt tcgcctgctg catcagcgca cgggcaatcg ccacacgttg ctgctggccg
     1621 ccggagaggg tggaaacgcg ctgatgggca aaatgcacca tgccaacgcg ggtcagcgcc
     1681 tgtaacgcgc gctgtttctg ctcgccggtg aaccagctaa aacaggtgcg ccagaacggc
     1741 gtgctgccga gcgcgccaat cagcacgttc tccagtacgc tcaggcggtt caccaggttg
     1801 aattgttgaa agatgtagcc ggtgttggcg cggcttttgc ggatatcgcg ggccaggcgg
     1861 ccttcgcgct ggacagtgcg gcccagtagc tcgatatggc taccggcgga tttatcgccg
     1921 gtaatcaaac cgcttaaatg acgtaaaagg gtggatttgc cggaacccga cggcccaagc
     1981 agagccacca tttcactgtg atgaatgttc agatcaaccg catgcagcgc ttgatgctga
     2041 ttgaaggttt tggcgagctt ctcgacacgg ataatcgttt gcatgatgca gcctccctaa
     2101 aaaagtggct ccatcgtggc ggattattgt gacgatttgg ttaattaaaa gtgactaaca
     2161 gatgaagagt taacgggaat tcgatgacag tgcgggaggg cgggcctccc ggcgggttat
     2221 tgcgttggtt gttgtttgac gacattaatc atccacggta cgccaaattt atcggtgact
     2281 ttgccaaagc catgcgccca gaaagtttcc tgccaggcca tttcgatttt tccgttagcg
     2341 gcaaggttat caaaccagcg ttttccttct tcgacctgtt gcgaatcgag caccagcgta
     2401 aagccggagt agctggcttt tcctgacggt atagcatcgc tcatcatgat gtcgcttccg
     2461 gcaatgcgca cgttggcatg agcgatggcg gtatcgggaa attgcattcc ggaagggcag
     2521 ttctcggcgc tgtcctgcgc tgatttcggc atttcgccga agctgatttt atagagcagt
     2581 tccgcgccca acgtacgttg ataataggca atcgcgtcgg aacagttacc ggcaaaagag
     2641 aggtagggac ttaacggcat gatggtgacc tcagttaaga gaagccagtt aagtgtagtt
     2701 cgaaatttat acagatgaga ggctgcctga taagacgcgc aagcgtcgca tcaggcagtc
     2761 ggcatcgttg ccggatgcgg cgcgagcgcc ttatccggct taccgatcac aatacaatca
     2821 gttctttttc acaaactcag atttcagttt catcggacca aaaccgtcga ttttgcaatc
     2881 gatgttatgg tcgccttcaa ccaggcggat gtttttcact ttggtgccaa ttttcagcat
     2941 cgaagagcta cctttcacct tcagatcttt aatgatggta acgctgtcgc cgtcagccag
     3001 cagattgccg ttagcatctt taacgatcag ctcgtcgctt tcctgtgcag gttctgcgtc
     3061 gttccattcg taggcacatt ccgggcagat gtacatgccg ttatcttcgt aagtgtattc
     3121 ggagttgcat tttgggcagt gtggtaatga catgtggatt tcctcaaaag tcagcagggc
     3181 aaaagcgcca aaaaacggca gattgccgaa aaaggccgca attatacaca aaatccctga
     3241 gtttgtcggg ggtactgctg attttagacg acgatcgttt tcatttgaaa ggaaacgcaa
     3301 aaagaaaata atctttttgc ctctatttat tatttaagat aatgttaatg ct
//
LOCUS       DAZSNO010000269         3325 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZSNO010000269 DAZSNO010000000
VERSION     DAZSNO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.273"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..208
                     /locus_tag="ACHWN2_004097"
     CDS             <1..208
                     /locus_tag="ACHWN2_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933380.1"
                     /translation="RHPDKRFIFNGNEIWKRGESTTEYTRAHQRTIRRLRRLIARKQY
                     TEKWFRKYDTWSAGYYELMATKPF"
     gene            269..457
                     /locus_tag="ACHWN2_004098"
     CDS             269..457
                     /locus_tag="ACHWN2_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933381.1"
                     /translation="MNIYFRIVISLAIIACIYGLIVPALISMKDTVAVISGFALACLT
                     PPCIYAIYKGLSFSKDKR"
     gene            454..1281
                     /locus_tag="ACHWN2_004099"
     CDS             454..1281
                     /locus_tag="ACHWN2_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR8933382.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="ACHWN2_004100"
     CDS             1325..2074
                     /locus_tag="ACHWN2_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR8933383.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="ACHWN2_004101"
     CDS             2071..2640
                     /locus_tag="ACHWN2_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933384.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2637..2759
                     /locus_tag="ACHWN2_004102"
     CDS             2637..2759
                     /locus_tag="ACHWN2_004102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933385.1"
                     /translation="MKIEYQDAAGGESMSWPDAIVTLGMVFAVAFVVYSICRWG"
     gene            2764..3006
                     /locus_tag="ACHWN2_004103"
     CDS             2764..3006
                     /locus_tag="ACHWN2_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR8933386.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="ACHWN2_004104"
     CDS             3010..3156
                     /locus_tag="ACHWN2_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933387.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHWN2_004105"
     CDS             3165..>3325
                     /gene="xisR"
                     /locus_tag="ACHWN2_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR8933388.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          928 a          796 c          855 g          746 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccggggt gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagcc
//
LOCUS       DAZSNO010000270         3282 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZSNO010000270 DAZSNO010000000
VERSION     DAZSNO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.274"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..98
                     /locus_tag="ACHWN2_004106"
     CDS             <1..98
                     /locus_tag="ACHWN2_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate transporter"
                     /protein_id="HFR8933389.1"
                     /translation="CAWLKRRLFYYTTPFFSSLLAFHKGPRLPDD"
     gene            101..1189
                     /gene="capU"
                     /locus_tag="ACHWN2_004107"
     CDS             101..1189
                     /gene="capU"
                     /locus_tag="ACHWN2_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR8933390.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            1194..2144
                     /gene="virK"
                     /locus_tag="ACHWN2_004108"
     CDS             1194..2144
                     /gene="virK"
                     /locus_tag="ACHWN2_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR8933391.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            2209..3153
                     /gene="lpxM"
                     /locus_tag="ACHWN2_004109"
                     /gene_synonym="msbB"
     CDS             2209..3153
                     /gene="lpxM"
                     /locus_tag="ACHWN2_004109"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR8933392.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT          914 a          626 c          764 g          978 t
ORIGIN      
        1 gttgcgcatg gctgaaaaga cgactttttt attatacaac cccgtttttt tcatcgttgc
       61 tggcctttca taaggggcca agattaccgg atgattaata atgaatatac tatttacgga
      121 atcatcaccg aatataggtg gtcaggaatt acaggctgtt gctcaaatga aggccctgaa
      181 gaaaatgggg cattcagttc tgcttgtctg cagggaaaac agcaaaattg cttttgaagc
      241 cagtaaattg gggattgata tcacattcgc gttatttcga aacagtcttc acatccctac
      301 tgcatggaga ttactcggaa tagttcatgg ttttcagccc aatgcaatcg tttgtcacag
      361 tgggcatgat agcaatattg ttggtttagt acggttattc actaggaaac atccattcag
      421 aattatcagg caaaagacat atttgacacg aaaaacaaaa gttttttcaa taaatcattt
      481 ttgcgatgag gtgattgttc ccggaacaag tatgaagaca catctggagc aggaaggatg
      541 tcgaacccgg gttactgttg tgcctccagg ctttgatttc cagaaattat acgttgattc
      601 ccgaaacagt ttgcctccaa atgttctttc ttggctggcg tcccgaaggg gatgccccgt
      661 tattgctcag gtaggaatgt tgcgcccgga aaaagggcac gaatttatgt tgaatttact
      721 gttccattta aaaatgaatg gacgacagtt ctgttggttg attgtggggt ctggttcgcc
      781 tgaactgcgg gagcatttac agtatcagat tgacagtatg ggcatgcatg atgatgtttt
      841 tattgctgac aatgtttttc ctgccgcccc cgtatatcgg gttgccagtc tggtggttct
      901 gccttcagaa aacgaatcgt ttggtatggt actggcagaa gcatcggcat tttctgtgcc
      961 tgtactggcc agtcagattg gtggaatccc tgatgttatt cagaataacc agaccgggac
     1021 attgttacca gcaggtaata agcacgcatg gatgtgcgcc ctgaatgatt tttttaatga
     1081 ccctgggcgt ttttatcaga tggctcgcca ggcaaaacag gatatagaag agcggtttga
     1141 tattaataaa actgcgttaa aaatactcac attagcgaag cacaagtaac aatatgtttt
     1201 ctgtaagtaa cttatcattt atcggtttcc ttaaaaggat tgttttttcc tcagattcac
     1261 tcccggggaa gtgggaacac agaaaattcc ggttcatgta cattttgcga tgtgctataa
     1321 atccggttgc cagtattcga tattattacg aactgcgttc cttgcagtgc attgaggata
     1381 ttctggcaat acaacccacg ttgccagcaa gaattcatcg accttatttg cataaagggg
     1441 ggcgagcctg gtcccggggg caatatattc ttgagcatta tcgctttgtg caaaatttgc
     1501 cagaaaaata ttctgaattt ttgtttccac aaaaatcggt atcattagtc caattcatcg
     1561 ggaaagatgg agaggatttt gatatccagt gttctcccag tggttttgac cgggaaggag
     1621 agttgatgtt gtcattgttt ttcaataaaa tagtgattgc ccggttaaca ttttcagtca
     1681 ttctgactca aaatggacac accgctttta ttggcggatt acagggagcc cccaaaaata
     1741 ccggacctga tattattcgg tgcgcaaccc gggcctgtta tgggcttttc ccaaaacgga
     1801 ttatttttga agcattttgt gcgttgatga aagcttgtaa tgtctctgag tgtctggcag
     1861 tcagtgagca cagtcatgtt ttccggcaat tgagatactg gtatcagaaa cgcaaaacct
     1921 ttgttgctgt ctacagtgat ttctgggagt ctgtagcagg gaaaacctgt ggtgactggt
     1981 acaaattacc aacacaggtg gttcgaaagc cgctaagcaa tatagctagt aaaaaacgct
     2041 ctgagtatcg aaaacgatac gctttgctgg attatatcca tgaaactgct atccgctctt
     2101 tggatgcata tcctgtaaac tcagaacatc aggacttaaa ttaaggtttg atgtattctc
     2161 tgaataaaat attaatgatg attttggtag gggcattcgc actaaataat gaaaaaatac
     2221 aaatccgagt ttattcctga atttaagaaa aattatcttt cccctgttta ctggtttaca
     2281 tggttcgttt tgggaatgat tgcaggtatt tcaatgtttc ccccttcatt cagagatcct
     2341 gtcttggcca aaatagggcg ttgggtgggc agattgagca gaaaagctcg tcgcagggcg
     2401 acgattaatt tatcgctttg tttcccggaa aagagtgata cagaacggga aataattgtc
     2461 gacaatatgt ttgccacagc attgcaatcc atagtgatga tggcagaact ggcaattcgt
     2521 ggtccggaaa agttccagaa acgagtgttc tggaaaggcc tcgaaattct tgaggaaatt
     2581 cggcacaata acagaaatgt gatttttctg gttccccatg gctggagcgt ggatattcct
     2641 gcaatgttgc tggcagctca gggggaaaaa atggccgcca tgtttcatca gcaacgaaat
     2701 ccagtgattg attatgtctg gaattctgta cggcgtaaat tcggggggcg cttacattcc
     2761 cgggaggatg ggataaaacc atttattcag tcagtacgcc agggatactg ggggtattac
     2821 cttccagatc aggatcatgg tcctgaatac agtgaatttg ctgatttttt tgcgacctat
     2881 aaagcgacat taccaattat tggacgtctg atgaacatca gtcaggctat gattataccg
     2941 cttttcccgg tttatgatga aaaaaaacat ttcctgacta ttgaagttcg gccaccaatg
     3001 gatgcatgca ttgccagcgc ggacaataaa atgattgccc gacaaatgaa caaaacagtg
     3061 gaaattttgg tggggtcaca tccggaacag tatatctggg ttttaaaatt gttaaaaacg
     3121 cgcaaatcaa acgaagcgga cccgtaccct tgaaattata aagtacaggt atttccacta
     3181 gttgtttctt acaggttacc aatcgaaaca catccccctt ccgtgtgttg cccgtttggc
     3241 acggaggact gtgtagatgc agttcgttaa atttaattta tg
//
LOCUS       DAZSNO010000271         3241 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZSNO010000271 DAZSNO010000000
VERSION     DAZSNO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.275"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            156..1635
                     /locus_tag="ACHWN2_004110"
                     /pseudo
     CDS             156..1635
                     /locus_tag="ACHWN2_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(1660..1998)
                     /gene="maoP"
                     /locus_tag="ACHWN2_004111"
     CDS             complement(1660..1998)
                     /gene="maoP"
                     /locus_tag="ACHWN2_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR8933393.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2117..2956
                     /gene="hdfR"
                     /locus_tag="ACHWN2_004112"
     CDS             2117..2956
                     /gene="hdfR"
                     /locus_tag="ACHWN2_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR8933394.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(3052..3127)
                     /locus_tag="ACHWN2_004113"
     tRNA            complement(3052..3127)
                     /locus_tag="ACHWN2_004113"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3092..3094),aa:Trp,seq:cca)
     gene            complement(3136..3212)
                     /locus_tag="ACHWN2_004114"
     tRNA            complement(3136..3212)
                     /locus_tag="ACHWN2_004114"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3176..3178),aa:Asp,seq:gtc)
BASE COUNT          877 a          790 c          797 g          777 t
ORIGIN      
        1 tcctttgtcc gctgcatttt tgcgagcatc attccaccgc cagaacaact caacttcact
       61 tgcacaaata aacaggaatc agccactaaa attcacgaat tagcgggcta cagcagcgat
      121 atcttatgca cataatggtg actcaaggag ggtttatgtc actgtcaatt gttcataccc
      181 gcgcagccct ggaagtaaat gcgcccccaa tcactgttga ggtacatatc agtaaaggtc
      241 tacccggctt aacgatggtg ggcttaacag aaacaacggt gaaagaagct cgcgatcgcg
      301 tgcgcagcgc cattatcaat agcggatatg aatatccggc gaaaaaaatc accatcaacc
      361 ttgctccagc cgatctgcca aaagaagggg gacgatatga tttacctatc gccattgcgt
      421 tgctggcggc ctcagaacag cttacagcca ataagttaga tgaatatgaa ttagtcggag
      481 aactagcgct tacaggcgct ctgcgtggcg ttcccggtgc aatctccagt gcaactgaag
      541 ctattaagtc gggcagaaaa attatcgtcg cgaaagataa cgaagatgaa gtggggctaa
      601 ttaacggtga aggatgcctg atagccgatc atctgcaggc tgtctgtgcg tttctggaag
      661 gtaagaacac tctcgaacgc ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg
      721 atctcagtga tgttgtcggt caggagcaag gaaaacgagg actggaaatt accgccgctg
      781 gtgggcacaa ccttttactg attgggccgc cgggaacagg taaaacaatg ctcgccagcc
      841 gtattaatgg cctgttgcca gatttaagca atgaagaggc actggagagc gctgcgatat
      901 taagtctggt aaatgctgaa tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac
      961 ctcatcacag tgcgtcgtta actgcgatgg tgggcggtgg tgcaattcca gggccaggtg
     1021 aaatttcgtt ggcgcataac ggcgtgcttt ttcttgatga gctacctgaa tttgaacggc
     1081 gtacactgga tgccttgcga gagccgattg aatccgggca gatccatctt tcacgcacac
     1141 gagcaaaaat aacctatcca gcccgtttcc agcttgttgc ggcgatgaat cccagcccta
     1201 ccggacatta tcagggaaac cataaccgct gcacgccaga acagacatta cgttatctca
     1261 ctggagatcc cattaccacc ccccggcatt ttgagtaaaa cggtagtgcc gggagaaagc
     1321 agcgccaccg ttaaacaacg cgtaatggcc gccagagagc gccaatttaa gcggcagaat
     1381 aaactgaatg cctggctgga tagtccggaa atacgccaat tctgcaaact tgagagcgaa
     1441 gatgcgatgt ggttggaaga aacgctgatc catctggggt tatcgattcg tgcctggcag
     1501 cggttactga aagttgcacg aaccattgct gatattgatc agtctgacat tatcacacgt
     1561 cagcatttgc aggaggcagt tagctatcgt gcgattgacc gtttgctcat ccatctgcaa
     1621 aaactactga cataaaaaaa gggcatttcg ccctttttat taatcgtcag aatcggtgta
     1681 gtcttcagca ccttcaacct gcggtttacc gccggaaagg gtgtgaaaac gttttggacg
     1741 cttaatacgc gtcatatact tggaccacac gcgttctgct tctgtcactg gctcacgttc
     1801 gccacggcat actgctacaa agagtttctc ttcctcggta accggctcgc gtttgccaag
     1861 atccaactca ttgaaggcat aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa
     1921 atcaccatga cgagagaatc cacgtggata atgtttattg tcgaaatatc gattagtcgt
     1981 cgtaaagctt tccgccatcc tgcacgctcc taattctttg accgagctag ttatggcgcg
     2041 gagtattagt tacgcttgac agagtgtaaa acaaaacatt taaatcataa cgacaaataa
     2101 ttttgcggag agcactgtgg atacggaatt gttaaaaact ttcctggaag ttagccgaac
     2161 gcgtcacttt ggtcgagcgg ctgaatcgct ctatctgacc cagtcagcag tgagctttcg
     2221 aatcagacaa ctggaaaatc aactgggtgt gaaccttttc acccgccaca gaaacaatat
     2281 ccgtttaacc gctgccggtg aaaaactact gccttatgca gaaacgctca tgagcacctg
     2341 gcaggccgcc cgcaaggagg tggcgcatac ctcacgacat aacgagtttt ctatcggtgc
     2401 cagcgcctcg ttgtgggaat gtatgcttaa tcagtggttg ggacgcttgt atcaaaatca
     2461 ggatgcccat acaggcttac agttcgaagc gcgaattgcc caacggcagt ctctggtaaa
     2521 acagctgcat gaacgccagc ttgatcttct tattaccact gaagcgccca aaatggacga
     2581 atttagtagt cagttgctgg ggtatttcac tttagcgctt tataccagtg ccccttctaa
     2641 actaaaggga gatcttaact atctgcgact ggagtggggg ccagattttc aacagcatga
     2701 ggcaggtttg atcggtgctg acgaagttcc cattctgaca accagttctg ctgaactggc
     2761 acagcaacag attgcgatgc ttaatggttg cacctggcta cccgtcagct gggcgcgtaa
     2821 aaaaggcggc ctgcataccg ttgtcgatag cacaacactt tcacggccgc tttatgccat
     2881 atggctgcaa aatagcgata aaaatgcgtt gattcgcgat cttttgaaaa ttaacgtgct
     2941 ggatgaagtg tattaatctg aatggctggc aaggatgccg gtagaaggat ttacttcgga
     3001 gaggattatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggcagggg
     3061 cggagagact cgaactccca acacccggtt ttggagaccg gtgctctacc aattgaacta
     3121 cgcccctaat tagggtggcg gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag
     3181 gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac caccggtttt
     3241 a
//
LOCUS       DAZSNO010000272         3239 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZSNO010000272 DAZSNO010000000
VERSION     DAZSNO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.276"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(61..588)
                     /gene="mobB"
                     /locus_tag="ACHWN2_004115"
     CDS             complement(61..588)
                     /gene="mobB"
                     /locus_tag="ACHWN2_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR8933395.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(570..1154)
                     /gene="mobA"
                     /locus_tag="ACHWN2_004116"
     CDS             complement(570..1154)
                     /gene="mobA"
                     /locus_tag="ACHWN2_004116"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR8933396.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1224..1493
                     /locus_tag="ACHWN2_004117"
     CDS             1224..1493
                     /locus_tag="ACHWN2_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR8933397.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1570..2556
                     /gene="srkA"
                     /locus_tag="ACHWN2_004118"
     CDS             1570..2556
                     /gene="srkA"
                     /locus_tag="ACHWN2_004118"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR8933398.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2573..3199
                     /gene="dsbA"
                     /locus_tag="ACHWN2_004119"
     CDS             2573..3199
                     /gene="dsbA"
                     /locus_tag="ACHWN2_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR8933399.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
BASE COUNT          826 a          777 c          790 g          846 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc gggtgcgacg ctggctgcgt ctgatccagc ctactcttgc
       61 ttatccgttc tgcttttgca tccactccac cacaaaatca gccagcccct caacatcatt
      121 aatatccagt aatgcgacat caagattaag cggcacatca ctggctacag caataacatg
      181 cctgtctatc actaattctt caggtcgatg tccggctcca tcgcgaaaca gcacaatctt
      241 tgcgatctct tcatgcttaa acccttcgac cagaatcaaa tccagctttg aggtatccat
      301 tcgacttgcg agaaaatgta gatccagctc ttcttcgtct ggtgtttccg tcatcaaggc
      361 ccatcgttgc tggctggcaa cgatggtttg taccgcgcca gccttgcgca gctcatagct
      421 atctttgcct ggcttatcaa catccatatc atggtgcgta tgcttaatca gccccggacg
      481 gatccctctg gcgcataatg ccgggatcag ttttttcaaa agcgtagttt ttccggtacc
      541 actccacgcg gcaaaggcga gtaacggtat catcgttttt cctgccatcg ggcaagctcc
      601 tctggcgtat tcacgttaac aaatgcatct ttatgatcgc tgaaatcaac cgcatgaccg
      661 ccagccagac gcataaatgc cattacccgg cgttctcctg cttgcagata ttccagtaat
      721 aaaggctcaa tagcgcggtt taccagagca atagtcgggt gatcgcgttc accgtcatgg
      781 acccacacga caggcgcatc tttgcgctga tgattaagcc gggcggctaa atcatgggga
      841 atgtaaggcg tatcgcacgg acaaaacaaa aaccactcac cagcttcctg ctgcattact
      901 gaaagcattc ctgccagagg gcctgggtaa tccgccagtg aatcttcaat cactttcaga
      961 ccgcctgcct gatagatttc ctgatgacga ttagcattaa ccacgacgtg agagagctgc
     1021 gtcttaagcg cgtcagcgac atgttgccac agcggtttgc catttaagtc cagcaatcct
     1081 ttatctacgc cgcccattcg tctggcttta ccgcctgcca gcacaacgcc tgttatcgtc
     1141 gtcattagat tcaccgatat cgcctctttt attgtgggat tgaccctgct aacgtgtctg
     1201 tctcaagagt aaggagcatt actatgaaat gtaaacgtct gaatgaagtt attgaactcc
     1261 tccagccagc ctggcaaaaa gagccagact ttaacttgtt gcaatttttg cagaaactgg
     1321 cgaaagagtc aggttttgac ggcgaactgg cggatttgac ggatgacatt ctgatctatc
     1381 acctgaaaat gcgtgattcc gcgaaagatg cggtgatccc gggtttgcag aaagattatg
     1441 aagaagactt caaaacggcg ctgttacgcg ctcgcggcgt aattaaagag taaaagcttg
     1501 taagcggcgc caccaaaatc atcgtgaaat gatatccttc atcattcgta atgttttccg
     1561 gatgatggga tgaacaacag cgcttttact ttccagacac tacacccgga taccatcatg
     1621 gacgctctgt ttaagcaagg gatccgggtg gattccggtc ttaccccgct taacagctat
     1681 gaaaaccgtg tctatcaatt tcaggacgaa gagcgtcgac gttttgtcgt caaattttat
     1741 cgccctgaac gttggacagc cgatcaaatc ctcgaagaac atcaatttgc gttgcagctg
     1801 gtaaatgatg aagttccggt cgcagcacct gtggccttta acggtcagac tttattgaat
     1861 catcagggat tttatttcgc tgtttttcca agcgtcggtg gtcgccagtt cgaagctgat
     1921 aatatcgatc agatggaagc ggttgggcgt tatttagggc gtatgcacca gacggggcgc
     1981 aaacagcttt ttattcatcg cccgaccatc ggtctgaatg aatatctcat tgagcctcgc
     2041 aagctgtttg aggacgctac actgatacct tccgggttga aagcggcatt tctgaaagcg
     2101 acagatgagc tgatcgccgc cgttacagca cactggcggg aagatttcac cgttctgcgg
     2161 ctacatggag actgccacgc cgggaatatt ctctggcgcg atggtccaat gtttgttgat
     2221 ctggatgatg cacgtaatgg tccagctatt caggatttgt ggatgttgct caatggcgat
     2281 aaagcccagc agcggatgca actggaaact attattgaag cttatgaaga atttagcgag
     2341 ttcgacaccg ctgaaatcgg actgattgaa cctttacgcg ccatgcgttt ggtttattat
     2401 cttgcctggc taatgcggca ttgggctgat cccgcgttcc cgaaaaattt cccgtggtta
     2461 accggggaag attactggct acgacagacg gcgactttta tagaacaggc aaaagttcta
     2521 caagaacccc ctttgcaatt aacacctatg tattaatcgg agagagtaga tcatgaaaaa
     2581 gatttggctg gcgctggctg gtttagtttt agcgtttagc gcatcggcgg cgcagtatga
     2641 agatggtaaa cagtacacta ccctggaaaa accggtagct ggcgcgccgc aagtgctgga
     2701 gtttttctct ttcttctgcc cgcactgcta tcagtttgaa gaagttctgc atatttctga
     2761 taatgtgaag aaaaaactgc cggaaggcgt gaagatgact aaataccacg tcaacttcat
     2821 gggtggtgac ctgggcaaag atctgactca ggcatgggct gtggcgatgg cgctgggcgt
     2881 ggaagacaaa gtgactgttc cgctgtttga aggcgtacag aaaacccaga ccattcgttc
     2941 agcatctgat atccgcgatg tatttatcaa tgcaggtatt aaaggtgaag agtacgacgc
     3001 ggcgtggaac agcttcgtgg tgaaatctct ggtcgctcag caggaaaaag ccgcagctga
     3061 tgtgcaattg cgtggtgttc cggcgatgtt tgttaacggt aaatatcagc tgaatccgca
     3121 gggtatggat accagcaata tggatgtttt tgttcagcag tatgctgata cggtgaaata
     3181 tctgtccgag gaaaaataat tcaatgtaaa ttacataaag cccgtgaata ttcacgggc
//
LOCUS       DAZSNO010000273         3208 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZSNO010000273 DAZSNO010000000
VERSION     DAZSNO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.277"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..338)
                     /locus_tag="ACHWN2_004120"
     CDS             complement(<1..338)
                     /locus_tag="ACHWN2_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR8933400.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="ACHWN2_004121"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="ACHWN2_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(992..1338)
                     /locus_tag="ACHWN2_004122"
                     /pseudo
     CDS             complement(992..1338)
                     /locus_tag="ACHWN2_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(1445..2008)
                     /locus_tag="ACHWN2_004123"
     CDS             complement(1445..2008)
                     /locus_tag="ACHWN2_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR8933401.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
BASE COUNT         1025 a          697 c          619 g          867 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattaga
//
LOCUS       DAZSNO010000274         3193 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZSNO010000274 DAZSNO010000000
VERSION     DAZSNO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.278"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            173..1276
                     /gene="proB"
                     /locus_tag="ACHWN2_004124"
     CDS             173..1276
                     /gene="proB"
                     /locus_tag="ACHWN2_004124"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR8933402.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1288..2541
                     /gene="proA"
                     /locus_tag="ACHWN2_004125"
     CDS             1288..2541
                     /gene="proA"
                     /locus_tag="ACHWN2_004125"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR8933403.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2656..2731
                     /locus_tag="ACHWN2_004126"
     tRNA            2656..2731
                     /locus_tag="ACHWN2_004126"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2689..2691,aa:Thr,seq:cgt)
     gene            complement(2746..>3193)
                     /locus_tag="ACHWN2_004127"
     CDS             complement(2746..>3193)
                     /locus_tag="ACHWN2_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8933404.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
BASE COUNT          755 a          807 c          878 g          753 t
ORIGIN      
        1 aaatgtggta atttattaaa tctgtaataa aagcgtaaac aactgccgct acgcttgctg
       61 atcccgcgca acaaaacgcc atgctttgct cgcagatggt tggcaaccga cgacagtcct
      121 gctaaaacgt tcgtttgata tcatttttcc taaaattgaa tggcagagaa tcatgagtga
      181 cagccagacg ctggtggtaa aactcggcac cagtgtgcta acaggcggat cgcgccgtct
      241 gaaccgtgcc catatcgttg aacttgttcg ccagtgcgcg cagttacatg ccgccgggca
      301 tcggattgtt attgtgacgt cgggcgcgat cgccgccgga cgtgagcacc tgggttaccc
      361 ggaactgcca gcgactatcg cctcgaaaca actgctggcg gcggtagggc agagtcgact
      421 gattcaactg tgggaacagc tgttttcgat ttatggcatt cacgtcgggc aaatgctgct
      481 gacccgtgct gatatggaag accgtgaacg cttcctgaac gcccgcgaca ccctgcgtgc
      541 gttgctcgat aacaatatcg ttccggtaat caatgagaac gatgctgtcg ctacggcaga
      601 gattaaggtc ggcgataacg ataacctttc tgcgctggcg gcgattctgg cgggtgccga
      661 taaactgttg ctgctgaccg atcaaaaagg tttgtatacc gctgatccgc gcagaaatcc
      721 gcaggcagaa ctgattaaag atgtttacgg cattgatgac gcactgcgcg cgattgccgg
      781 tgacagcgtt tcaggcctcg gaactggcgg catgagtact aaattgcagg ccgctgacgt
      841 ggcttgccgt gcgggtatcg acaccattat tgccgcgggc agcaagccgg gcgttattgg
      901 tgatgtgatg gaaggcattt ccgtcggtac gctgttccat gcccaggcga ctccgcttga
      961 aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt gaaatcacgg tagatgaagg
     1021 ggcaactgcc gccattctgg aacgcggcag ctccctgttg ccgaaaggca ttaaaagcgt
     1081 gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc aacctcgaag gtcgcgatat
     1141 cgcccacggt gtcagtcgtt acaacagcga tgcattacgc cgtattgccg gacaccactc
     1201 gcaagaaatt gatgcaatac tgggatatga atacggcccg gttgccgttc accgtgatga
     1261 catgattacc cgttaaggag ctggccgatg ctggaacaaa tgggcattgc cgcgaagcaa
     1321 gcctcgtata aattagcgca actctccagc cgcgaaaaaa atcgcgtgct ggaaaaaatc
     1381 gccgatgaac tggaagcaca aagcgaaatc atcctcaacg ctaacgccca ggatgttgct
     1441 gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc gtctggcact gacgcccgca
     1501 cggctgaaag gcattgccga cgatgtacgt caggtgtgca acctcgccga tccggtgggg
     1561 caggtaatcg atggcggcgt actggacagc ggcctgcgtc ttgagcgtcg tcgcgtaccg
     1621 ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg tgacggttga tgtcgcttcg
     1681 ctgtgcctga aaaccggtaa tgcggtgatc ctgcgtggtg gtaaagaaac ctgtcgcact
     1741 aacgcggcaa cggtggcggt gattcaggac gccctgaaat cctgtggctt accggcgggt
     1801 gccgtgcagg cgattgataa tcctgaccgt gcgctggtca gtgaaatgct gcgtatggat
     1861 aaatacatcg acatgctgat cccgcgtggt ggcgctggtt tgcataaact gtgccgtgaa
     1921 cagtcgacaa tcccggtgat cacaggtggt ataggcgtat gccatattta cgttgatgaa
     1981 agtgcagaga tcgctgaagc attaaaagtg atcgtcaacg cgaaaactca gcgtccgagc
     2041 acatgtaata cggttgaaac gttgctggtg aataaaaaca tcgccgatag cttcctgccc
     2101 gcattaagca aacaaatggc ggaaagcggc gtgacattac acgcagatgc agctgcgctg
     2161 acacagttgc aggcaggccc tgcgaaggtg gtggcggtta aagccgaaga gtatgacgat
     2221 gagtttctgt cattagattt gaacgtcaaa atcgtcagcg atcttgacga tgccatcgcc
     2281 catattcgtg aacacggcac acaacactcc gatgcgatcc tgacccgcga tatgcgcaac
     2341 gcccagcgtt ttgttaacga agtggattcg tccgctgttt acgttaacgc ctctacgcgt
     2401 tttaccgacg gcggccagtt tggtctgggt gcggaagtgg cggtaagcac acaaaaactc
     2461 cacgcgcgtg gcccaatggg gctggaagca ctgaccactt acaagtggat cggcattggt
     2521 gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa aagtagccat ttgattcaca
     2581 aggccattga cgcatcgccc ggttagtttt aaccttgtcc accgtgattc acgttcgtga
     2641 acatgtcctt tcagggccga tatagctcag ttggtagagc agcgcattcg taatgcgaag
     2701 gtcgtaggtt cgactcctat tatcggcacc atttaaatca ataagttaca catcattagt
     2761 accttcctta tttcttgact gggacaaatt tgggaccgat gggttcagaa tcaagtctat
     2821 ttgccgtgcg tgttcggtaa ggtgattagg tgcgaggtga gcatatcgac gaaccatttc
     2881 gatagactcc cagcctccca tttcctgtaa cactgataac gggactccgg cttgaaccag
     2941 ccaacttgcc caggtgtgtc tcaagtcgtg aaatctgaaa tcatcaatac cagcccgtct
     3001 cagcgccgct ttccaggctg tgtttgcgtc ataccgcatc ttccttactg ttggcgcttt
     3061 cgttccgtct ggtttggtac agctttcctt gtacacaaat acccaacggt gatgatttcc
     3121 gatttgtttt ttcaatacgc gacatgcagt atcattcagc gcaacgccaa ttgcgcggtt
     3181 tgatttactc tct
//
LOCUS       DAZSNO010000275         3190 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZSNO010000275 DAZSNO010000000
VERSION     DAZSNO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.279"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            223..882
                     /locus_tag="ACHWN2_004128"
     CDS             223..882
                     /locus_tag="ACHWN2_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR8933405.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1083..1460)
                     /locus_tag="ACHWN2_004129"
     CDS             complement(1083..1460)
                     /locus_tag="ACHWN2_004129"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8933406.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1527..>3190)
                     /locus_tag="ACHWN2_004130"
     CDS             complement(1527..>3190)
                     /locus_tag="ACHWN2_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR8933407.1"
                     /translation="GFIKPRWQKLVMTDAGIDRRYYELCALSELKNSLRSGDIWVQGS
                     RQFKDFEDYLVPPEKFTSLKQSSELPLAVATDCEQYLHERLTLLEAQLATVNRMAAAN
                     DLPDAIITESGLKITPLDAAVPDTAQALIDQTAMVLPHVKITELLLEVDEWTGFTRHF
                     THLKSGDLAKDKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAWHTRDETYST
                     ALAELVNAQFRHPFAGHWGDGTTSSSDGQNFRTASKAKSTGHINPKYGSSPGRTFYTH
                     ISDQYAPFHTKVVNVGLRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLG
                     FRFAPRIRDLGDTKLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDEILRLATSIKQGT
                     VTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEAR
                     NALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHA
                     VDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          687 a          827 c          920 g          756 t
ORIGIN      
        1 gacgcacttt gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat
       61 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt
      121 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
      181 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      241 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      301 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      361 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      421 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      481 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      541 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      601 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
      661 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
      721 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
      781 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
      841 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
      901 tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc
      961 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg
     1021 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct
     1081 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata
     1141 tgatcattta ttctgcctcc cagagcctga taaaaacggt tagcgccggg gttggatttt
     1201 tcagcgttcc agctaaggct aaggcattcc tgttcaagcg caagccgggc tataaagcgc
     1261 attatcgctt tacccgtgcc cttatttcga tcgcactgag aaacatacag ctctttaata
     1321 tgcagctgac cgctgtatcg gggcgaggga taaagaatat tgcaacatgc caggccagta
     1381 atgttattgc cgcagcgcgc tctgatcacc agggtaccgg aaagccggtt gaataacttt
     1441 ttacagagat aatccttcat caacgcttcc tgaatgatac cttcgccata ataggggtcg
     1501 tctcagaaaa cggaaaataa agcacgctaa gccggttgca gaggccgtag cggcctgaac
     1561 ttccccgcgc cgatcttggc gctgctgcgc cataggtaat caccggtcag gttgatgtgc
     1621 tcccagccga gtggcgacag gtactgcaat agcgagtcat cgacggcatg accattgccg
     1681 cgcaacgcat gcgccgcacg ctccaggtag accgtgttcc acagcacgat ggccgccgtc
     1741 accaggttga ggccgctggc ccggtagcgc tgctgctcga aactgcggtc acggatttca
     1801 ccaaggcggt tgaagaacac ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc
     1861 ccggcatgca cgcggcggcg tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg
     1921 cgctcgatgc ggcccaactc gcgcagcgcg acggccaagc cgttctggcg cgggtagctg
     1981 ccgagtttcc tgagcatcag cgaggccgtc accgtgccct gcttgatcga ggtggccagc
     2041 cgcaggattt cgtcccaatg ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc
     2101 ggcttgagcg cgtcataggc ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg
     2161 tcgcggatgc gcggcgcgaa gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg
     2221 gtgaagcccg ccgtgtcggt gtagtgctcc tcgatccgca ggtcggattc gtggtacagc
     2281 aggccgtcga gcacgtaggt tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat
     2341 ggcgcgtatt ggtcggagat gtgggtgtag aaagtccgtc ctgggctgct gccatatttt
     2401 gggttgatgt gccccgtgct ctttgccttg ctagcggttc ggaaattctg tccgtccgat
     2461 gatgatgtgg tgccatcgcc ccagtgcccg gcaaagggat gccgaaactg agcgttgacc
     2521 agttcagcca acgctgtcga gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg
     2581 agcttcgcgt aggtcgtgcc ggggcaggac tcggccatct tggtcaggcc caggttgatc
     2641 gcgtcggcca ggatcgtggt caacaacagg ttcttgtcct tggccagatc gcccgatttc
     2701 aagtgcgtga agtgccgggt gaagcccgtc cactcatcga cttcgagcag cagttcggtg
     2761 atcttgacgt gcggcaggac catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc
     2821 accgccgcat ccagcggcgt gatcttcaag cccgactcgg tgatgatggc atccggcagg
     2881 tcgttggctg ccgccatgcg gttgacggtg gcaagttgtg cttccagcag cgtcagccgc
     2941 tcatgcagat attgttcgca gtcggtggcc acggccagcg gcaattcgct ggactgcttg
     3001 aggctggtga acttctcggg cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa
     3061 ccctgcaccc agatgtcgcc cgagcgcagg gagttcttca actcggacag cgcgcacagt
     3121 tcgtagtagc gccggtcgat gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg
     3181 atgaagccgg
//
LOCUS       DAZSNO010000276         3185 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZSNO010000276 DAZSNO010000000
VERSION     DAZSNO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.280"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..320
                     /gene="iraM"
                     /locus_tag="ACHWN2_004131"
     CDS             <1..320
                     /gene="iraM"
                     /locus_tag="ACHWN2_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR8933408.1"
                     /translation="DTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFTLTHTGI
                     MLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQICPYGLKQ
                     QSP"
     gene            complement(601..709)
                     /locus_tag="ACHWN2_004132"
                     /pseudo
     CDS             complement(601..709)
                     /locus_tag="ACHWN2_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1232..1308)
                     /locus_tag="ACHWN2_004133"
     tRNA            complement(1232..1308)
                     /locus_tag="ACHWN2_004133"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1272..1274),aa:Arg,seq:tct)
     gene            complement(1410..1485)
                     /locus_tag="ACHWN2_004134"
     tRNA            complement(1410..1485)
                     /locus_tag="ACHWN2_004134"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1450..1452),aa:Ile,seq:cat)
     gene            complement(1630..2451)
                     /locus_tag="ACHWN2_004135"
     CDS             complement(1630..2451)
                     /locus_tag="ACHWN2_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR8933409.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(2466..2822)
                     /locus_tag="ACHWN2_004136"
     CDS             complement(2466..2822)
                     /locus_tag="ACHWN2_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR8933410.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(2835..>3185)
                     /locus_tag="ACHWN2_004137"
     CDS             complement(2835..>3185)
                     /locus_tag="ACHWN2_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR8933411.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT          877 a          872 c          623 g          813 t
ORIGIN      
        1 ttgatacagt tatccagcca tcatgcggaa tatctttttc agtcatatgg agtaaaataa
       61 aattaataat ctggtatcaa tcggatgctt tcttacctcc tgaaagtata tttacactga
      121 ctcacacagg catcatgctc aataacaaag tgctacctgt aaccatttac aacgtagtac
      181 cattcaataa aacattctgg aatttaatca aaaacagcca ggaatgccct acaaatacag
      241 ataacgtatt gaatgaatgc tttaataacc gttgcactct gcaaatatgc ccttatggac
      301 taaaacaaca aagtccataa ggagtttact cacatctgac aaaatcaata taaacagccc
      361 ctccggagag gggctggaga gtggcgctat gtgccattcc atggtgccgg gtgcctcccg
      421 gtgaattcag taccagcacc tgaatccgcg attatcccat atacctactc gctgattgcc
      481 cctccgcaca gggggattca ccatgccagt ttcttttaac aaactccccg caaaccagac
      541 aacagtcaac cgcctgaatt gtgaagtatt taaaaatttc tcccgctaac tgatacccgg
      601 ctaacagtct ggcgttttct ttttcagcaa cgggaaagca acaaccacca cacccgccac
      661 cagcacaccg tcagccggca ctgacattat ccggctgctg caatgccatt aacaaaaaca
      721 gtaagcaatc actttttacc gtaacaggtg ataatccaga tatgtatcta ccccagatga
      781 gtaatccgaa gttcatccat accacaggtc ctggctattc tgttgtactc ctgaacaaga
      841 gcaaataatt ctgaattagc aaccatgaac tcatcgcaaa ccctctgtat agcatcacta
      901 ttcagaataa taacgtctct tcccgaaaga cgatcaggag tacagaacaa aactgtcaaa
      961 cggctgaagg cctttgctcg tgctgcattg actatatcaa tacgctgcct aaggatgaaa
     1021 caccccgacg cctcatcaat attcactcta cccacaccat atgaatgata aatacttaat
     1081 gctgaaaaaa ccattagacc gtataacaaa tactcaatca atacttaaca gaacttttat
     1141 ttttgacaaa tatataatat tttcaacaat atcctgagcc aggtatattt cagtataagg
     1201 ctctgccgga aggaatctgg aagaatgaat atggcgcgct gtactggatt cgaaccagtg
     1261 accgattgct tagaaggcaa ttgctctgtc cggctgagct aacaacgctg aataccgata
     1321 atggaccgcc atcggggacc cgccccccgc accaacaacc ctgttatcgt gtcgtctgct
     1381 cttcctgata agctaatggc ggtttgtgat ggtggccctt gctggatttg aaccagcgac
     1441 ctggcgatta tgagtcgctc gctctcacca ctgagctaaa gggccgggag cagaataata
     1501 acggtccgta attaatttcg caataaaaaa acccgctcag cggcgggttg tagaaactct
     1561 tctaacgcca ggcataaaac gcccatcatt atgacgaatt taccacagat tccggaaaaa
     1621 tcaaccttgt tacctagtta ccttttttaa ctgccgctca gcccatgctt cttcaatatc
     1681 aaaccgggtc accagcgcat cataaaattt cttaactgtt ttttcccatg acgcgcgtgt
     1741 tatctggttt gtcacctcgc atatagcatt aaatgcctcc gttgatggta gtctttcata
     1801 gccacgacca ccacaacgct ggcagtctct gataacaggc ataccacgtt ttaccgactc
     1861 ttcacgatga atggcgacac cacgcccacg gcaatcctta caggcggtgg aaacctcacc
     1921 ctttccgcca cactccggac aggcaacttt taccacctcc ctgacttttt tccattcttc
     1981 ccagtaaaac ggatacacac ctttcgtaca ctttgcccat accggcggct taccatccgg
     2041 atactggatc ttgtttgtaa aaactatgct ttcaataaat ttttccccat agcaacaagg
     2101 gcactgcttt ttactcgctg cgctgcgggc ataatcctca aaagcgtacg aagccataat
     2161 gcgcatcact accggtttta tttctgccgg aagttttctc aacgccgcca cacgatcgca
     2221 ccgactgagt gcataatctg ccagtaattc tgttgcccgc gccctgtcat tcatactgat
     2281 gcccattttc cccaggaacg cagaaaaacc catctcagcc cgattctgtg tcatgccctg
     2341 cgcggccatc acatcagtga tactcagcgc atcttttgac gttgaggccg atgcatcggt
     2401 caggccaggg gattttgggg agtagtattt cggtaaatct tccagtttca ttttttgacc
     2461 tgctcttaat gcattatggg gtaaatcttc acccccagac gtccaccaga tactggctga
     2521 ccacgaacga tattgatttc atcaaactgc tcatcgtcca ttaacactcc cgcatgcgtc
     2581 agcgcatcca gcggtgcttt caggatattg tccaggtcgc gacgacgctt atccggtggc
     2641 tctgcaatca cctttatcgc cagccttccg gacaggctta atttcagccg ctgctggcga
     2701 acaataagcg ccacagcccg gcgataacgc tttccctcct ccgagataaa atatgtgctg
     2761 ccacggcgtc gccagtaagt gttcaccgtc ggcgggtaag gtaaaaccaa atctatgagc
     2821 atcagtcacc tcttttaccc aagcacgcca gttgcaaagg cgtgatcaag aaaacgaaaa
     2881 attaaatcaa cctgagaacc atgcttttct tcgaacgcca gaggatccgc atgaagctcg
     2941 ttgtgatgct cccgacacag cggtagcgtg aaaatatcgt gagatttggt ccccattccg
     3001 ccctgaccat gaccaatcag gtgatgggga tcgtcggctg gcttaccaca acacgcacac
     3061 ggctgtgtct tcacccagcg tttgtatttc tcgttaaccc agcggcgacg tttaggtcgt
     3121 ttcatgaaag attccggaga ctcaggatca acggcaatgc tgaccaccgt cttttcctgt
     3181 tgtgg
//
LOCUS       DAZSNO010000277         3180 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZSNO010000277 DAZSNO010000000
VERSION     DAZSNO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.281"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHWN2_004138"
     CDS             418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHWN2_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR8933412.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1772..1906)
                     /locus_tag="ACHWN2_004139"
     CDS             complement(1772..1906)
                     /locus_tag="ACHWN2_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR8933413.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2552..2722)
                     /locus_tag="ACHWN2_004140"
     CDS             complement(2552..2722)
                     /locus_tag="ACHWN2_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR8933414.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
BASE COUNT          800 a          659 c          776 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg ggttttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg gggggggacc ctgagcaggt ggtaattgta agacgttgcg
     2821 ggcgacggta ttacaattgc acatcctgtc ctgtttcttt aggacgtatc atggcacagc
     2881 atatcaactg ataaataatc cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg
     2941 cgtcgagtgt ttcttaatca ataattccgc tgatactccg gaaaaacgcg agtctgttct
     3001 catgcctgaa atgcccacac cccacgcaaa aacaagtttt tgctgatttt tctttataaa
     3061 tagagagtta tgacaaatta gttcttcttg ctctctttgt gatatttaaa aaagcggtgt
     3121 cggcgcggtg ttgtagctgc gccaacaccg ctttttaggg gtggtactga ctattttcat
//
LOCUS       DAZSNO010000278         3178 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZSNO010000278 DAZSNO010000000
VERSION     DAZSNO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.282"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            95..2248
                     /locus_tag="ACHWN2_004141"
     CDS             95..2248
                     /locus_tag="ACHWN2_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR8933415.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..>3178
                     /locus_tag="ACHWN2_004142"
     CDS             2301..>3178
                     /locus_tag="ACHWN2_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933416.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQN"
BASE COUNT         1158 a          570 c          584 g          866 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacag
//
LOCUS       DAZSNO010000279         3094 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZSNO010000279 DAZSNO010000000
VERSION     DAZSNO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.283"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..749)
                     /locus_tag="ACHWN2_004143"
     CDS             complement(<1..749)
                     /locus_tag="ACHWN2_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFR8933417.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIF"
     gene            942..1445
                     /locus_tag="ACHWN2_004144"
     CDS             942..1445
                     /locus_tag="ACHWN2_004144"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8933418.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1491..1907)
                     /gene="rraB"
                     /locus_tag="ACHWN2_004145"
     CDS             complement(1491..1907)
                     /gene="rraB"
                     /locus_tag="ACHWN2_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR8933419.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            2069..3082
                     /gene="argF"
                     /locus_tag="ACHWN2_004146"
     CDS             2069..3082
                     /gene="argF"
                     /locus_tag="ACHWN2_004146"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR8933420.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          930 a          712 c          676 g          776 t
ORIGIN      
        1 tcgaagataa tataaccagc aacagcgaga aaaggtaata aagctaaaaa tgcgaggatg
       61 gcatatttta tacaatacgc agtatctaat ttcaccttga aacgatgtat accgaaactg
      121 gtattgctcc agagaaaact cattaccagg ctatataaag taccattaaa aataccaatg
      181 gaaacaatac caactattgc catcagaacc acagatataa taacactact tgaactattg
      241 gcatgtagca tctttgtcga gataaagaaa acagtcccca tcccaatggc cattaaaatc
      301 ggcaagaaaa aggttaccca ccagaaccct ttcaaagagc aattaaaact aaatcttacg
      361 ccgttgagac tggtcattaa ggcctgataa cgcagtcctt ttgctgccat aaatataagc
      421 aaaaccaaca gtaacaaagt caaaacacag ccaacaagcg gcatatctgc tgacactgtc
      481 attaagattg cgaaatagaa aaaaacaaaa acaagacaac taacaaaaac attcccaccg
      541 gtaattccat aagaaaatcg ttgtccgtta acttccatat tagcataaag ataacgctta
      601 catttcatta atgcccatgg taaatagata cctagcgtta taatcgttaa caacacattc
      661 accacacaaa taagaaaata tctctcacct gtaccatgaa aaacaaaaga atgggacgga
      721 acatccattt cattaataac ttgagccata aaaatcccct gaattaaaaa atccattgat
      781 ataatttata gatacagaca gtaaaatgtt ctgccacgtt acaatactct tatcagtaat
      841 aggctggcaa aaactttatt gctttataag acgcttgcgc acgtaagaag agagtggtat
      901 taaacaaatg tataattact ttatttttat tgggttaaac tatgaataac attgcgccgc
      961 aatcacctgt aatgcgtcgc ctgacgctgc aggataatcc tgctatcgcc agcgtcatcc
     1021 gtcaggttta cgcagagtat ggtcttaccg ccgataaagg ctacaccgtt gccgatccga
     1081 atctcgacga actgtatcaa gtgtacagcc agcctggcca tgcttattgg gtggttgaat
     1141 acaatggcga agtggtcggc ggcggcggga ttgcgccatt aacaggtagt gaagcggaca
     1201 tttgcgaact gcagaaaatg tactttcttc ccgctattcg cggtaaaggg ctagcgaaaa
     1261 aactggcctt aatggcgatg gagcaggcac gagagatggg cttcaaacgc tgctatctgg
     1321 aaacgaccgc ttttttaaag gaagccattg cgctttatga gcatttgggc tttgagcata
     1381 tcgactatgc gcttggctgc acgggccatg tcgattgtga agtgcggatg ctacgcgaac
     1441 tctaaaaaaa tgcccgctag tatgtgctat cgggcatttt aatcaattaa ttagtggcga
     1501 accccgtcat cgtcttcatc gacaaaatct tcatcgtcgc catcttcgcc gttaggatct
     1561 tcaaagtaag tgccccaacc gtcgtactca acgtcaaatt tctccgccag cgtcatcagt
     1621 tgttcaacct gggcatcgat cagatcggca ttcaacgcgc actcgctgag gatgtcgcag
     1681 caaatcacga tatcaccatc ttcaacttcc agctcttctg gatcggtcac ttcgtaaccg
     1741 agtttaaacg cttcaactgc cgctttttcc agagtttcca gatcgtctgc ggaaagatgg
     1801 tgttcgatgg tgtacagcgc gtccggatcg ctgccatctt ccagtaattc ttcaataatc
     1861 aaacgtgttt cttcacgctg ttcttccagt tgttccgggt ttgccatggc tcattcctct
     1921 taatgtcctg ccgatactct tattgtcaca caccactgac attgcctcca cctttgtgac
     1981 aaagatttat gctttagact tgcaaatgaa taataataca cataaattga gttttaattc
     2041 attgaggcgt tagccacagg agggatctat gtccgggttt tatcataagc atttcctgaa
     2101 attactcgat ttcacgccag ctgaactcaa cagcctgctg cagttagccg cgaagctgaa
     2161 agctgataag aaaagcggta aagaagaagc caaactcact ggtaaaaaca tcgcgctcat
     2221 cttcgaaaaa gactcgactc gtacccgatg ctctttcgaa gttgccgcat atgaccaggg
     2281 ggctcgcgtt acttatcttg gcccaagcgg tagtcagatt ggtcataaag agtcgattaa
     2341 agacactgcc cgcgtgcttg gtcgcatgta tgacggtatt cagtatcgcg gctatggtca
     2401 ggagattgtc gaaacactgg cggaatacgc tggcgtgccg gtatggaacg gcctgaccaa
     2461 tgagtttcac cccacgcagt tgctggcgga tctcctcacc atgcaggagc atttacccgg
     2521 taaagcattc aacgaaatga cgctggtcta tgcaggtgac gcacgtaaca acatgggcaa
     2581 ttcgatgctc gaagctgcgg cgcttaccgg tctggatttg cgtctggtcg cgccacaggc
     2641 atgctggcca gaagctgcgc tggttacgga atgccgcgcc ctggcacaac aaaatggcgg
     2701 gaatattacg ctgacggaag atgtagctaa aggagttgaa ggtgctgact ttatctatac
     2761 cgatgtatgg gtgtcgatgg gtgaagcaaa agaaaaatgg gctgaacgca ttgcattact
     2821 gcgtgattat caggtgaaca gcaagatgat gcagttgacc ggtaacccgg aggtcaaatt
     2881 cctccattgc ctgcccgcgt ttcatgacga ccaaacgacg cttggcaaaa aaatggcgga
     2941 agagtttggc ctacatggcg gaatggaagt gactgatgag gtcttcgaat ctgccgccag
     3001 cattgtattt gatcaggcgg aaaaccgcat gcataccatc aaagcggtga tggtcgcgac
     3061 gctcagtaaa ttaaataatt aatatcgcta taaa
//
LOCUS       DAZSNO010000280         3069 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZSNO010000280 DAZSNO010000000
VERSION     DAZSNO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.284"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..498
                     /locus_tag="ACHWN2_004147"
     CDS             <1..498
                     /locus_tag="ACHWN2_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8933421.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="ACHWN2_004148"
     CDS             578..1273
                     /locus_tag="ACHWN2_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR8933422.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="ACHWN2_004149"
     CDS             1302..>3069
                     /locus_tag="ACHWN2_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8933423.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPSTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          889 a          718 c          683 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccagtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAZSNO010000281         3023 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZSNO010000281 DAZSNO010000000
VERSION     DAZSNO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.285"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(312..455)
                     /locus_tag="ACHWN2_004150"
                     /pseudo
     CDS             complement(312..455)
                     /locus_tag="ACHWN2_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            426..710
                     /locus_tag="ACHWN2_004151"
     CDS             426..710
                     /locus_tag="ACHWN2_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933424.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            complement(712..1284)
                     /locus_tag="ACHWN2_004152"
     CDS             complement(712..1284)
                     /locus_tag="ACHWN2_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933425.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(1370..1576)
                     /locus_tag="ACHWN2_004153"
     CDS             complement(1370..1576)
                     /locus_tag="ACHWN2_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8933426.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(1962..2753)
                     /locus_tag="ACHWN2_004154"
     CDS             complement(1962..2753)
                     /locus_tag="ACHWN2_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933427.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
BASE COUNT          826 a          628 c          714 g          855 t
ORIGIN      
        1 aatggcgttt atatgaattt tatcatctta ctctgaatgt tcatttcatc agtgtgacat
       61 tccattgttt actgataaag ttagccgata aataatattg ttgagattat tgaaacggca
      121 gttttcgccg attcagtgca atggaagaga tgttatatca tctatttaag caataagttt
      181 aatacaagtt tcagaagagg tgttggtgag ctgatgactg attttcagag ggatatggaa
      241 aagtgtatag aatcccataa aaaatcgata ttgttcagtt gacgtatatt tctggaggat
      301 gactccagtc atcagaacat atgtttttcg atgaatgacc caataatttt ttcgtgaatt
      361 tccacagcac gtgaaaagta aatcgttttt cggtccagtc gtttgatgcg ggaacggagt
      421 gtcagatggt ggtgctcaat acgccgggta aatatcaata cgatttgagg aaccatttgc
      481 tggttaatct ctttccgata gcattggatg cagaaataac tacatccaat gtaaataagc
      541 atcaattgca cagaagcaat atacctgcgg ccctctccgg aaattccggt atcactgact
      601 acgtaccgtt ttcagcggaa cgaaatgcgg cgatggtcat ttggcggaca gccatagata
      661 gccgtccggc tgtgtgtaac gatgaggaag gaaccccggg aacgttatga atcagcaggc
      721 gtataacagg tactcgtcaa tcatcagttc cagctcgcgc ttttcccgtt tgcgcgccac
      781 agcccagttt tcaagctctg catgtgattt atgctttaac tcttcagtcc agggctgcat
      841 ggagtgggca cacatcagat cataccagca cagccatatc agcgtgtggt agagatcacg
      901 atcgtcgaaa cgccggagca ggctgttcag cctgtttgcc agccggtttt cacccagacg
      961 atgtagctca ctgagtaaaa cgccgcccag attaaaatgt gtaagcagca tgatttcatg
     1021 ggtctgaatg ctggtcatta acccttcctg aatggcagga atgaacggtg gtgttcccgg
     1081 gttgacgacc tgatagagtc cctggggggc caccgaaatg acggagcctt tttccggcag
     1141 aaaaaagtgt gccagggtca gacggtgata accatgaaga tgcggagacc ccattccatg
     1201 agcgacgata agcctcgtca cgcttgtcat ccatttctga tgctggatga gcagcaggtc
     1261 acacctgttt tcaagttgtt gcataatatt ctctccttta gcgcgcagca aaatgcctcc
     1321 gggggccgga tcatgccttt gctgcggtgg aacggataac ggtcaggaat tacggacggg
     1381 actgtgcaat acgcgcctgc agccaggctt ccacttcgct tttcagccag cgggagctac
     1441 ggcccagttt gatggggggc ggaaaggctc catgcttgat gagtttataa aaccacttat
     1501 cggtcaggcc ggtcagctga gtgataaacg acatgtcgac catctggtca tccatcagtg
     1561 aaactggggt attcatgaga atgttcctcc ggatgttgtt aatctcaacc ggcgttggtg
     1621 atgaaaacga cgcaccacct tcaccggcat aacaaccggg gagaaacgta aaaaatgagt
     1681 aggggaatat tcctgccagg agagaaactg ttgtgtacag gcaatacagg ttgtctgttt
     1741 tgtcagtgaa tccggtataa acggaagatg attttgtgtt gccggaatat gggatggcgt
     1801 tttgccgtga cgggatcagt attttcagtg atgattatta ctgcacaggg gagtatcgga
     1861 gataaaccac gccctgtacc tgccagagcg gtggtttgta tttactgaac ggaagataaa
     1921 gcagaggtaa acactgtcgc ggtgaaggta gaaggctgaa attattctct gtaattacaa
     1981 acaacagtga tcttatccgg tttttttacc gttacgggtt tcagagggtc aattttatat
     2041 acaggggtaa tgagcacgcc ggaatttatg gaaaaaagag ggatccagca ataaacgtcc
     2101 ggatatgtct gtgtctccgg aatacctttt gcgccagaga ccatcagatc tgtaacgctc
     2161 acaaaaaatg actgccgcaa atcaaaaaag tactcgttat atatcacatc ctttgccagc
     2221 aggatgactg cattaccaac agccgctttt acaggtattt tatcagatgg attatcgtga
     2281 ttgtatattg ttctttcgat ataaataaaa caggcatatt tcgtcttctc cccggatagc
     2341 tgcatctggt attcgcaaat gtctttgata caccgacttt caacccttcc aatcatctta
     2401 tcccggcgat agtgtctttt atgtttcagc gcgtacagaa caccatttga actgtcaaac
     2461 tccaggattc ttctggtgaa aagtttgttt tcccagattt ccagacaata cttctctgca
     2521 ctgacgacat tgcgtttgcg aggaagatcc cgttgataca ctccatttaa taagcgtgac
     2581 tgcagttcgc ctgcaagttg ataataacag ttaatatcat taaggacagg tgctgtggcg
     2641 ctgagtaatg cgttgattac cttagagcgc gaaagtttgt gggtatgaca gtactcatcc
     2701 acaattcgca gcgtctcatc actggtcata ctgaacgtta tacgtgttgt catgatgaag
     2761 ttttctgtta atcatattgg ttgagatgca taacatttca taaaaaaacc tgttaattca
     2821 tgcaattaat tttttaatga attttatcct caagtgatgg aaagcagtgt tactgccact
     2881 gtactcagta taactctatc acacagaatc acagaaaatg aagcattgct cagggggaca
     2941 ctcatacgag ttctctgaac tgtgataacc agtatttttc attttgggtg atttgtttat
     3001 cgttacagct acaggtagtt cat
//
LOCUS       DAZSNO010000282         3013 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZSNO010000282 DAZSNO010000000
VERSION     DAZSNO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.286"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1278)
                     /gene="icsB"
                     /locus_tag="ACHWN2_004155"
     CDS             complement(<1..1278)
                     /gene="icsB"
                     /locus_tag="ACHWN2_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR8933428.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLV"
     gene            1592..>3013
                     /locus_tag="ACHWN2_004156"
     CDS             1592..>3013
                     /locus_tag="ACHWN2_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HFR8933429.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAGVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLS"
BASE COUNT          985 a          427 c          630 g          971 t
ORIGIN      
        1 tactaagtat gagtggcaag cgttgatgac cgcatcaaag tcttctgaag atacattttc
       61 agtaggatgt ttgtaaagtg aaattgataa aggcttgaat gtttttggtg tgtggataag
      121 gttaatttta ttgaaacttt taagatagct gcgtcgtaat tgagtcaatc tggaaagatc
      181 aaaatcagat tcaatatact tcattatttc gttataagac tgattgagac tttcaatgcg
      241 ttgcctaact ttatttgcat acgcgtatac atcgttaggt gtagcaacca atttaacgtc
      301 agggagtgga aagtatattt cagaatttcc tgctttaaga acattaagtg ccataccagc
      361 acagttttca gatttactga ttaatttata atttgccaat tgtctgaaag catctgcttt
      421 ctttttgcat ataaattttg acatgtttgt ttcatcaagg ccaaaaaggt tatggctgat
      481 tttaccatcc tttgttttat tatcacctga taatggaatg tatactttat cagcactgac
      541 cccccaataa ccataggggt cttttttttg attagctctg ggaaaaaata tttttttgtt
      601 gttattttga tcttgttttg aatttaataa gtttctaacc tcttggcctg catcaagtct
      661 ttcggctgtt tggtctgaaa tcatatttag tttgtcttgc ttataggtat cgacactgta
      721 acctgagcgt ttttcaaaaa ggtagtttat ttctgtatct tttgtaacat tcccgccggg
      781 ataccagctg agataggtat gagttggttc ctgtagtaaa ttgtttttta tactgatact
      841 agtatgccct aaatgggtta tgcctctatt tccccagcaa cgaaaaacta aatgtctttc
      901 aagatttttg gctgtaccga tgccatgaaa acgctctgcg gtattcaaca ttaatttgat
      961 gtctctacct gtgaaagagt gttgaattga gtttttagct gcttcatagg cggcaaacgc
     1021 actttctgca gatttttcgc tagctaatgt attgaggaaa agcaataagg atttttgatt
     1081 ctctagtcga taatttttta gttgttcatt taatatctgt gaatttatct ttgcattaat
     1141 agtgcttaat gttctgaaaa agagatcctt taggttgaat ttctgtgcta tcaatatagg
     1201 tccatgctct gtatcttcaa cgcgtatagg cccttttgta ttgcttgcgt caatgaaatt
     1261 gctaattttg aggatcatac tttattaact ctccattact tggtgattgt ggggatgtaa
     1321 aatgtatttg gtatataata tattcaatta ataaatttag aaacttgagc ctgttaacat
     1381 aatcaaattt tcttttgctg tacataatat gtacctcgtg agcatatgta gtgctcgttt
     1441 catcatgaaa tcccacaaga taaagtgcct gatgtatcag gctcggagtg ttatagaaaa
     1501 agagagaacc ctgttgaata agtatctttt ttgttgtaaa acgaggtaca aaactgcaat
     1561 caatgcccca tttatgactg aggataatta aatgcacata actaatttgg gattgcatca
     1621 ggtttcattt caaagcggag attcctataa aggcgcagaa gaaaccggaa agcacaaagg
     1681 tgtaagcgtg atttcatatc aaagagttaa aaatggagaa agaaataagg ggatcgaagc
     1741 gcttaatcga ttatatttac agaaccaaac aagcttaaca ggaaaaagcc ttttgtttgc
     1801 gcgtgacaga gctggagttt tttgcgaggc aataaagttg gcaggtggtg atacgtcaaa
     1861 aattaaagcc atgatggaac gattagatac ctataaactt ggtgaagtta ataaaagaca
     1921 tattaatgag cttaataaag taataagtga agagataaga gcacagctag gcattaaaaa
     1981 taaaaaggaa ttgcaaacga aaataaaaca aatatttact gattatttaa ataataagaa
     2041 ttggggaccg gtcgacaaga atattagtca tcatgggaaa aattatggtt ttcaattaac
     2101 tcctgcctct catatgaaga tagggaataa aaatatattt gtcaaagagt ataatggaaa
     2161 aggaatttgt tgtgcttcta cgagagagag tgatcatatc gcgaatatgt ggctttcaaa
     2221 agtggtggat gatgagggga aggaaatttt ttcgggtatc agacatggag ttatatcagc
     2281 ctatggattg aaaaaaaact catctgaaag agctgttgct gctcgtaata aagctgagga
     2341 gttagtaagc gcagcattat atagcagacc agagttatta tcacaggctt tgtctggtaa
     2401 aacagtagat ttaaagattg tttcgacttc tcttctgacg ccgaccagtt taaccggggg
     2461 agaggagagt atgttaaaag atcaggtgaa cgcattaaaa gggttaaact ctaaaagggg
     2521 gaagccaacg aaattattaa ttcggaatag tgatggtctt ctgaaagaag taagcgttaa
     2581 tctaaaagtg gtgacattta actttggtgt aaatgaatta gcgctaaaaa tgggcttagg
     2641 ctggaggaat gttgacaagc ttaatgatga atcaatatgt tctttgttgg gggataattt
     2701 cctcaaaaat ggtgtgattg gaggttgggc tgcagaagca atagaaaaaa atccaccatg
     2761 taagaatgat gtgatatatc tggctaacca gataaaagag attgtaacca aaaagttaca
     2821 aaaaaatgat aatggagagc cgtataaatt gtcacaaaga atgactctat tggcttatac
     2881 tataggtgct gtaccttgct ggaattgtaa gagtgggaag gacagaacag gtatgcagga
     2941 tgctgaaatt aaaagagaaa taataagaaa acatgaaaca ggtcagtttt ctcaattaaa
     3001 tagtaaatta tcc
//
LOCUS       DAZSNO010000283         3006 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZSNO010000283 DAZSNO010000000
VERSION     DAZSNO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.287"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHWN2_004157"
     CDS             complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHWN2_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR8933430.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            348..641
                     /locus_tag="ACHWN2_004158"
     CDS             348..641
                     /locus_tag="ACHWN2_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR8933431.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="ACHWN2_004159"
     CDS             713..1372
                     /locus_tag="ACHWN2_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR8933432.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="ACHWN2_004160"
     CDS             1464..1910
                     /locus_tag="ACHWN2_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR8933433.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3006)
                     /gene="hlyE"
                     /locus_tag="ACHWN2_004161"
                     /pseudo
     CDS             complement(2117..3006)
                     /gene="hlyE"
                     /locus_tag="ACHWN2_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT          784 a          649 c          720 g          853 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacg
//
LOCUS       DAZSNO010000284         2951 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZSNO010000284 DAZSNO010000000
VERSION     DAZSNO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.288"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="ACHWN2_004162"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="ACHWN2_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR8933434.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHWN2_004163"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHWN2_004163"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR8933435.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="ACHWN2_004164"
     CDS             2603..>2951
                     /locus_tag="ACHWN2_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR8933436.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAZSNO010000285         2937 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZSNO010000285 DAZSNO010000000
VERSION     DAZSNO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.289"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(238..1662)
                     /gene="patD"
                     /locus_tag="ACHWN2_004165"
     CDS             complement(238..1662)
                     /gene="patD"
                     /locus_tag="ACHWN2_004165"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR8933437.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1684..2478)
                     /locus_tag="ACHWN2_004166"
     CDS             complement(1684..2478)
                     /locus_tag="ACHWN2_004166"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR8933438.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2468..2815)
                     /locus_tag="ACHWN2_004167"
                     /pseudo
     CDS             complement(2468..2815)
                     /locus_tag="ACHWN2_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT          758 a          849 c          679 g          651 t
ORIGIN      
        1 ccgtgaattt gtacaattga actttcattg ttaatacttc tacttataaa gtattagttt
       61 aataacgata ctctaaagcc ttatgctaat caactgtgac aggatgaaaa atcgccatca
      121 aacatttagc ctaattttta cgccagttat taatgtaatc attctaatac ttaaccgcca
      181 ttaaactcag aacctaaaac gatagtggcc gtcctataac agccactaac atctaactta
      241 atgtttaacc atgacgtggc ggacgacggt gtaatcctcc agcccataaa gtgacatatc
      301 cttgccgtaa ccagaaagtt tctgcccacc gtgcggcatt tcacttacca gcatgaaatg
      361 ggtattgacc caggtacaac catattgcag ccgtgcgctg acgcgatgcg ccctgcccac
      421 atctttcgtc cataccgaag atgcaagtcc gtactggctg tcattcgccc agttcaccac
      481 ctgttcttcg ttgtcgaagg gcgtaacact cactactgga ccaaatacct ctttttgcac
      541 gatggcatcg tcctgtaatg cgccagccag cagcgtcggc gcatagtaat agccattacc
      601 cttgcgcttt tcaccgccag tgatcacttt gatgtgccct gtcgctttcg cctcttctac
      661 tgccttgctg acgcgttcga gatgcgccag cgagcttaaa ggtccaagct ccgtagactc
      721 gtcatccggc gcaccagatt ttaacgttgc caccgcagca cccagttttt ccaccagcgt
      781 atcgtaaatg cctttttgcg cgtagatccg acaagccgca gtacaatcct gtccagcatt
      841 gtaatagcca aatgtacgta caccttcgac cactgcttca atatccgcat catcaaaaac
      901 aatcactggc gctttgccac caagttccat atgagtacgc ttaatggacg acgcggtatg
      961 gctgatgatg tgctcgccgg tggcgataga gcccgtcagc gacaccatcc gcactttggg
     1021 atgaccggtc agcggatcac ccaccgtttt gcctctgcca aacagtatgt taatcacgcc
     1081 tgccgggaag atatctttcg ccagctctgc caacttcaac gcggtcagcg gggtaatttc
     1141 tgatggttta agcactacgc agttccctgc cgccagcgcc ggagcaagtt tccacgcggc
     1201 catcatcagc ggataattcc acggtgcgat agaagccacg acccccaacg gatcgcgacg
     1261 gatcatcgaa gtatgacctt caagatattc acctgccgcc agaccattca gacagcgcgc
     1321 cgcacccgcg aaaaagcgaa aaacatcgac aatcgccggg atttcatcat tgaacgcact
     1381 atgcagcggt ttgccacaat tacgggattc cagttcggca aaaacctgac cattttcttc
     1441 gataacatcc gccagtttca gcaggcattc cgcacgcgct ttcggtgtgg tttgccccca
     1501 ttcagcaaat gctgcatctg ccgcacgcac agcagcatcg acctgctctg cggatacctc
     1561 ggcaatctcc agtaaaacgt cccccgttgc cggattatag acaggctgtt tttccccttc
     1621 gccgctaacc cgttctccgt taatcagtaa cttatgttgc atagcttatt cctgtatcag
     1681 tggttattga ccattgtcgc cttcgcgggt tagccaccag gcccccagga tcggcaaggt
     1741 tgttaccaac ataaccagca gtgccaccac gttggttacc ggtacatcac gcggtcgccc
     1801 aagctgattg agcaaccaca acggtaacgt tcgttcatga cctgccgtaa aggtcgtaac
     1861 gatgatttca tcgaacgaca aggcaaacgc cagcattcct cctgccagta acgccgaact
     1921 gagattcggc aacactacgt agcggaaggt ttgccagcca ttggccccaa gatccattga
     1981 cgcctcaacc agactccacg aggtgcggcg aaaacgggcg atgacattgt taaacaccac
     2041 cactacgcaa aaagtcgcat gaccgaccac gatggtgaaa aatcccggct ccagattgat
     2101 ggttttaaag gcggttaata gcgccagacc agtgacaatg cccggcagcg caatgggcag
     2161 cagcaataac agcgaaatgg cgtttttgcc gaaaaagtct cgtctccaca gcgcggcagc
     2221 tgccagcgtc cctaacacca gcgcaattaa tgtcgccagc gccgccactt taagtgacag
     2281 cgtcacggca tcaagaatat cactacgctg tgctgccacg ctaaaccagc gcagcgtcag
     2341 gccctgcggt ggaaaactaa acgccgcatc ttcagtgtta aacgcatagg cggcgattat
     2401 caggatggga aaatgtagga aaacaacgcc gccccaggcc gccagtttga ggaaaaacgg
     2461 tgcgcgttca gagtgcatcg aacgctccca gacgtttcac gaacgccagg tacagtgcga
     2521 tgagaataat cggcaccagg gtgaatgccg ccgccatcgg catattgcca atcgccccct
     2581 gctgggaata aaccatattg ccgataaaat atcctggagg accaaccagc tgcgggacga
     2641 taaaatcgcc cagtgtgagt gagaaggtaa agatagagcc agcggcaata cccgggattg
     2701 ccagcggcag caccacatag cgaaaggttt gtcgtggacg tgcgccgaga tcagccgacg
     2761 cctgcaacaa tgacggcggc aaacgctcaa gcgccgcctg aacgggcagg atcatcgagt
     2821 aggcagcctg acggctgcgg cttgtcatgg tctgcaatta ccgttataaa aacaggcaat
     2881 gtcattgtct ttcaggtggt tatatggccc gttcagctaa accccgtaaa cgtaaac
//
LOCUS       DAZSNO010000286         2932 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZSNO010000286 DAZSNO010000000
VERSION     DAZSNO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.290"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="ACHWN2_004168"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="ACHWN2_004168"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFR8933439.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="ACHWN2_004169"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="ACHWN2_004169"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFR8933440.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="ACHWN2_004170"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="ACHWN2_004170"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFR8933441.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="ACHWN2_004171"
     CDS             complement(1895..2809)
                     /locus_tag="ACHWN2_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFR8933442.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          773 a          681 c          781 g          697 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattaccta gaaccacggc agcctgacgg ctgcaaattg aaaactgccc ct
//
LOCUS       DAZSNO010000287         2932 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZSNO010000287 DAZSNO010000000
VERSION     DAZSNO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.291"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACHWN2_004172"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACHWN2_004172"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR8933443.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACHWN2_004173"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACHWN2_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR8933444.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHWN2_004174"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHWN2_004174"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFR8933445.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2932
                     /locus_tag="ACHWN2_004175"
     CDS             2756..>2932
                     /locus_tag="ACHWN2_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933446.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          660 a          800 c          805 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cacatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DAZSNO010000288         2898 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZSNO010000288 DAZSNO010000000
VERSION     DAZSNO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.292"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..312)
                     /locus_tag="ACHWN2_004176"
     CDS             complement(<1..312)
                     /locus_tag="ACHWN2_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR8933447.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DI"
     gene            complement(359..1078)
                     /gene="btsR"
                     /locus_tag="ACHWN2_004177"
                     /gene_synonym="yehT"
     CDS             complement(359..1078)
                     /gene="btsR"
                     /locus_tag="ACHWN2_004177"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR8933448.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="ACHWN2_004178"
     CDS             complement(1075..2760)
                     /gene="btsS"
                     /locus_tag="ACHWN2_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR8933449.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          750 a          774 c          658 g          716 t
ORIGIN      
        1 aatgtcatcg gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt
       61 aatgcgacgt tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat
      121 caggccattg aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc
      181 ttctttacgt agccagacgg cgatctgttc cgcggtggct tcgacattat ccagcgccag
      241 aatacgcacc aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc
      301 gttacttatc atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt
      361 acaggccaat cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc
      421 cattacgcaa aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg
      481 cgaggttaac cagatactga cgatggcagc gcagtagtgg tgtacgactt tccaaggtac
      541 gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca
      601 tccgactgct gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg
      661 tacaagggat aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc
      721 tgcgctcctg acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca
      781 gcagataatc aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga
      841 gaaaaacaat atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga
      901 tgcgcggcat ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga
      961 tcccttccac ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa
     1021 atatacgcag gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc
     1081 ctcgtccctc catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac
     1141 gcttattcca tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc
     1201 cagcccactg gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat
     1261 caaatgttgc ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga
     1321 tgtcccatgt ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg
     1381 ctgggataat tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt
     1441 ttcaatttgc agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga
     1501 aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg
     1561 ttcgctgtcg cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg
     1621 attcacctgg gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc
     1681 atattgccct gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg
     1741 gtttattgaa ctgaataacc ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg
     1801 ctgattttcg ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa
     1861 agagcaacga taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc
     1921 ttttaaggtg taagtcgaag aaatcggttt gccgggtaaa tggtggtcgt caccaattcc
     1981 ggtaaaggcc agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg
     2041 atacagtacc tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc
     2101 ttccgtcgag gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa
     2161 catcgcgcgt ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac
     2221 catcattggc gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat
     2281 cgcaaggatg atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat
     2341 ggggttaaag actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc
     2401 gagcaatcct tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga
     2461 atatcgatgt aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa
     2521 gccgcccatt accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc
     2581 aaaccaggtg cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg
     2641 cagacgaacc gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc
     2701 aatgactaaa aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat
     2761 acccgcaaac cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct
     2821 ttgaactctt gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa
     2881 aataaaatca ccctatag
//
LOCUS       DAZSNO010000289         2834 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZSNO010000289 DAZSNO010000000
VERSION     DAZSNO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.293"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHWN2_004179"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHWN2_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR8933450.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACHWN2_004180"
     tRNA            complement(621..695)
                     /locus_tag="ACHWN2_004180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="ACHWN2_004181"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="ACHWN2_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHWN2_004182"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHWN2_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR8933451.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          679 a          693 c          683 g          779 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccga
//
LOCUS       DAZSNO010000290         2807 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZSNO010000290 DAZSNO010000000
VERSION     DAZSNO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.294"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..81
                     /locus_tag="ACHWN2_004183"
     tRNA            6..81
                     /locus_tag="ACHWN2_004183"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHWN2_004184"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHWN2_004184"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHWN2_004185"
     CDS             complement(299..1057)
                     /locus_tag="ACHWN2_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR8933452.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHWN2_004186"
     CDS             complement(1065..2168)
                     /locus_tag="ACHWN2_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR8933453.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGVCWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="ACHWN2_004187"
     CDS             complement(2178..>2807)
                     /locus_tag="ACHWN2_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFR8933454.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          764 a          739 c          674 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acagacaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAZSNO010000291         2798 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZSNO010000291 DAZSNO010000000
VERSION     DAZSNO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.295"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1611)
                     /locus_tag="ACHWN2_004188"
     CDS             complement(<1..1611)
                     /locus_tag="ACHWN2_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR8933455.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIK"
     gene            complement(2043..2762)
                     /locus_tag="ACHWN2_004189"
     CDS             complement(2043..2762)
                     /locus_tag="ACHWN2_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFR8933456.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          862 a          539 c          466 g          931 t
ORIGIN      
        1 ttttattatt ctacaatact ctaatattgt ttctttgtct ctatttacta cagatacaaa
       61 tacaccagga ataccatctt tattagttgc ggacagcatt tcgaccaatc gtcttgtggt
      121 aaaactatag ttttttagaa tattacaaaa ggcacgtatt gtttcggtat ggccattata
      181 aaatgccata aaaagtcctg atgtaccgtt gggatttttt atccataata attcatctat
      241 atattcctgg tgagggggga gtatttttag tatatctcca tacgccataa tggcctttgt
      301 gtgcccattt tgaagtgcca taaacaaccc atgctttact ttcaataatt ctttaatttg
      361 ttcactacta agcattttct tattaagcag acttccgaac atacgaatac tatctgcatg
      421 tccattctgc aatgcaaaaa ataacccagg taaatcatgg ctgtctttcg cagctaatag
      481 atataatctc ttatcggaag ttaacggggt tttaaagact atttccccat aggcttttat
      541 tgtgtctaca tgtccgttca taaaagacat gcataaaccc ggcacattgt ttgcccctac
      601 cgcagcaagc aatgtaagta ttgtttcttg actaagattg cacatattta atatcttacc
      661 atatacttga ataatgtctg aatatccgtt ttgaagtgct atataaaatg caggagtacc
      721 atcagagctt ctcgtggcca gaaggttcac cagtttagat tgagaaattt caccattttt
      781 gtatttttcc atcagtataa ataatacttc tgtcagattc tttgttatcg catgatatat
      841 aactctggaa ttgatcaatt catctggtaa aagtttcact gtgtttgtta gaggggaatt
      901 atgcctatct tcaaatatta cctgcccggg ttgctccaga ccataaattt tccaataaaa
      961 atcatcaaaa aaatccttag cggataattg actcagttcg aaatggctat cacaagtaaa
     1021 tacagtacgt ttatgagtaa cagttcgatt aggatcgaaa aactgaacaa cccatttttc
     1081 actttcttgt acgcgcttta ttttcaaccc taacgccatt tggtggttta atgtgcatgc
     1141 tgataatgtt ctgacaaagt ctccattttt tttcatgtta taaaaaaaat tagtaataac
     1201 tgttccccac tttttatttt ccacaaaata aatataagct ggtgattttg aaaaatcttc
     1261 cgttttttcc aaacatacat tttttgcaat agcatcaggg gtgctaagaa gctgatagtt
     1321 taccttccca tttgtttcca taaaattcat ttcccaatag gaagctaggt gacggcaaac
     1381 tatttgtctt ccatatatat cttttgccac tccatttata tttacctcca catttctctt
     1441 ttctgaaaaa taaggtgctg ttttattctt aaactgaatt agttcactgg agtttgagac
     1501 cctcataata gaacttgtta ttgttctatt atctctgtta acagacatat ctgttgataa
     1561 cgaatttttt gttgaaaata tactagatga aaatgtttta tttaacggca tagaagacaa
     1621 ccatagagat gttataatac tattagattg cagttactaa tagtaaaagc ctccttacaa
     1681 ggaggtagct tattcagaat gctcctataa gatagcgagc tcgtcaacga tacagatgat
     1741 ctgattatat tgtgcaaaag gagaacagga aaataggcac tggaaaagat gaaggcctga
     1801 tgggagcatt aactaatgca aaatccgaca ggttaatatc ccgaaaagca atcaaacgac
     1861 tcaaacagga gatgcacgct gaaatggtgg acaactgttc ggatttcaga tgagtagctc
     1921 agcagtttat gagccgaatg ctctgtcacg ggcaggaatc aggctgcata cgcagtgcca
     1981 aatctaatcg tagttattct aaccagtttt caagctacaa ggtggtgtag ctggcatctt
     2041 ctctactcta tcatcaaacg ataaaatggt tcctctcgta acatttgctc ttgcctttca
     2101 cttccatctc gatcgcttcg tagttcatta cgatagctga catatttcca gtcttcaggt
     2161 ctaacatctg acgccggata ctccccggga gcgattttgc ttcttaatag attagactcg
     2221 agacacatta taaattgtcg tattttatga agtaataaag cactatattg cgagcattcc
     2281 tgatctgatt ttacatatag cgtaaactga gctcctatcc ccacacgaga ttgctgagaa
     2341 actcgattca tatcagttat cttccattta tctataggac tgtcctctga aaataataaa
     2401 ccagacaaaa tttggaatgc cagaggaact tgctccctag ctatactaat atgaaacttg
     2461 tccccaacaa aatccccctt actctgagga ttttcacgac atgcatgaat aaacacatct
     2521 ccactatggt tcacaatctg gaatccttgg tacatgacag aaaaagattg acatatatca
     2581 gagtaagttg gcacgttcga tgctactttg aaatagtacg ctgggtattg ccgtatttgg
     2641 ttgtacaata tattgaagtt atttttcaat ctttcattcg cagaaagcat ctgcggtatt
     2701 tcagacctca caacattcaa agaatctaaa tttagtttta gacagggctt ttttatgggc
     2761 atagaaaacg tcctctataa aatagatata ttatctta
//
LOCUS       DAZSNO010000292         2774 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZSNO010000292 DAZSNO010000000
VERSION     DAZSNO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.296"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            66..584
                     /gene="mddA"
                     /locus_tag="ACHWN2_004190"
     CDS             66..584
                     /gene="mddA"
                     /locus_tag="ACHWN2_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR8933457.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            581..1030
                     /locus_tag="ACHWN2_004191"
     CDS             581..1030
                     /locus_tag="ACHWN2_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR8933458.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1031..1264)
                     /gene="ydcY"
                     /locus_tag="ACHWN2_004192"
     CDS             complement(1031..1264)
                     /gene="ydcY"
                     /locus_tag="ACHWN2_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR8933459.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1350..1523)
                     /gene="ortT"
                     /locus_tag="ACHWN2_004193"
     CDS             complement(1350..1523)
                     /gene="ortT"
                     /locus_tag="ACHWN2_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR8933460.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1718..1813
                     /gene="yncL"
                     /locus_tag="ACHWN2_004194"
     CDS             1718..1813
                     /gene="yncL"
                     /locus_tag="ACHWN2_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR8933461.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2215..>2774)
                     /locus_tag="ACHWN2_004195"
     CDS             complement(2215..>2774)
                     /locus_tag="ACHWN2_004195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFR8933462.1"
                     /translation="PTCHFSGNIRIGRFCSIASNVKIMGGNHPLNRFTTHMMTYNGEF
                     DKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDVVLKGGIAIGDGAVIAANSVVTK
                     DVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNYNYSDLPDNNKCDDINYFVEEMN
                     RLISNGNIQERDYKKFNLSEVFRGL"
BASE COUNT          727 a          618 c          610 g          819 t
ORIGIN      
        1 cgttgcttcg ggctgttttc gatagtattg ataacatcat catcattatt ttattgaggc
       61 cagacatgtc catccgtttt gcccgcaaag ccgactgtgc tgccattgcg gaaatttata
      121 accacgccgt gttgtatacg gcggctatct ggaatgacca aacggtggat gcagataacc
      181 gcattgcctg gtttgaagcg cggaccatag caggttatcc ggtgctggta agtgaagaag
      241 atggcgtagt tacgggatat gcctcgtttg gcgactggcg tagtttcgat ggttttcgcc
      301 ataccgtgga acattcggtt tatgtccatc ccgatcatca gggcaaaggt ctggggcgta
      361 aattgttaag tcgattgatt gatgaagcgc gggattgcgg gaagcatgtc atggtcgccg
      421 ggatcgaatc gcaaaatcag gcttcgctgc atctgcacca gtcgctggga tttgtcgtca
      481 ccgcacaaat gccgcaggta ggcactaaat ttggtcattg gctggatctg acatttatgc
      541 agttgcaact cgacgagcgc actgaaccgg acgcgattgg atgaatcagt cgctcaccct
      601 tgcttttttg attgccgccg gaattggtct ggtagtccag aacacgctga tggcgcgcat
      661 cacccagacc tcctctacca ttctcatcgc catgttgctg aactcactgg tggggattgt
      721 gctctttgtt tctattttat ggtttaaaca aggcatggcg gggtttggcg aactggtgtc
      781 cagcgtgcgc tggtggacac ttatacctgg cttgctgggt tcgttttttg tcttcgccag
      841 tatcagcgga tatcagaatg tgggagcggc gacaactatt gcggtactgg tcgccagcca
      901 gctgattggc ggtctggtgc tggatatctt ccgcagtcat ggcgtaccac tacgggcgtt
      961 gttcggccca atatgtggcg caattttact tgtggttggc gcctggttgg tggccagacg
     1021 ctcattttaa tcagaggatg gtgccgcctt tggttaactg ttcgtggcgc gcttccatat
     1081 cttctttatg cttgcgaccg tgatgagcaa tagcggtgcg caaacgctgc tgctgggtgt
     1141 agcgatcttc ccgacttaac tccgcgtcat cgctcagggc gattaataat tcgttcatat
     1201 gggcaatgac gctctcttca atcgcggcat ccacgcgcgc gatgacttca tccagatgcg
     1261 acatactttc tccttagcca cttgtgaaat ttatcgatta atccaaatga tataagtctg
     1321 cgtggtgccg tcaagccagt gagtaagatt taaaacagtg aaaacaacag cgccaccggg
     1381 aaactcatcg gccatgtggc ccccaccaga aaggcgctta agaaacggat gcgtttctta
     1441 tcgtgagaaa gaaaccaggt gataagaaat gcgattgctg ccataaccgc ataaaaaaca
     1501 agcatatgtt gatagagaaa catgtacggg cgtccagcca tatgaataaa acgcgcgcaa
     1561 atatgcgcca ttttttgacg tgcatcaagt tttttcacca gtttgagaaa aaatcatcaa
     1621 acagcaactt gcccttatcc atcttcagaa tgagtattat tgcggatttt cttaacccgt
     1681 actatacaca taggggcaat gataaaaggt ggcaaaaatg aatgtttcca gtagaactgt
     1741 agtactgata aatgtctttg ctgctgttgg tttgtttact cttatctcta tgagatttgg
     1801 ctggtttatt tgatgtagtg attttgcacg cagcacgtac tgtcagagaa atcaactctc
     1861 tgacagtacg caagatcttc tcatactctc caagtttgca acattatcct taatatttac
     1921 ttctttttgc tggcatctac attgaaatga gtagaaaaca tccccctact gtcgtctata
     1981 caaacagctt actgattcaa acggaatctt gttttcacat ttttctatca cgaatacttt
     2041 atacgcgcgg gagtctataa atgctttata ctttcaagga ataacgtaaa tataaaaaga
     2101 tggcgtaaag tatatgcatg gcaggatgtt tgcaacctcc ttgatgacgc tgccccattg
     2161 aaattgcaaa ccccgccctg gttatcttgc ccatttatca tgcaaaacag ataattatag
     2221 gcctctgaat acctcagaaa gattgaattt tttatagtcc ctctcttgaa tatttccatt
     2281 tgatatcaat cgattcatct cttcaacaaa ataatttata tcatcacact tattattatc
     2341 cggaagatct gagtagttat aattccacca ttttattctc aacagttcat caataacatt
     2401 tgaatcaaac ctaaatctga ttatttttgc aggaactcct gcaactatgg catatggggg
     2461 cacatctttg gtaacaacgg aattggcagc aataacagca ccatcaccta ttgcgatgcc
     2521 acctttcaat acaacatcat ttcctatcca gacatcattt cctataatcg gattttcagg
     2581 tttggtaatg aatggtttca atgtccagct acgttcaaat tcactcatag caaacttatc
     2641 aaattcaccg ttataagtca tcatgtgagt agtaaaacga ttcaaaggat gatttccacc
     2701 cattattttt acatttgatg caatactaca aaatcgacct atacgtatat tccctgaaaa
     2761 atggcaggtc ggaa
//
LOCUS       DAZSNO010000293         2733 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZSNO010000293 DAZSNO010000000
VERSION     DAZSNO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.297"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..593)
                     /locus_tag="ACHWN2_004196"
     CDS             complement(<1..593)
                     /locus_tag="ACHWN2_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR8933463.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHWN2_004197"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHWN2_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR8933464.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVINNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="ACHWN2_004198"
     CDS             complement(1422..1961)
                     /locus_tag="ACHWN2_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8933465.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHWN2_004199"
     CDS             complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHWN2_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR8933466.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          708 a          699 c          601 g          725 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattattta tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
     2701 cattttgact catagaggaa agcatcgcgc aca
//
LOCUS       DAZSNO010000294         2711 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZSNO010000294 DAZSNO010000000
VERSION     DAZSNO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.298"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            50..2572
                     /gene="lpfC-O113"
                     /locus_tag="ACHWN2_004200"
     CDS             50..2572
                     /gene="lpfC-O113"
                     /locus_tag="ACHWN2_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR8933467.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2583..>2711
                     /locus_tag="ACHWN2_004201"
     CDS             2583..>2711
                     /locus_tag="ACHWN2_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8933468.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          726 a          585 c          677 g          723 t
ORIGIN      
        1 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
       61 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      121 cgaggtcagt gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat
      181 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      241 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      301 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      361 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      421 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca
      481 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
      541 taatgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
      601 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
      661 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
      721 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
      781 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
      841 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
      901 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc
      961 acaaggtgct tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt
     1021 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1081 gattcttaaa cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1141 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1201 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1261 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1321 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga
     1381 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1441 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     1501 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     1561 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     1621 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     1681 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     1741 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     1801 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     1861 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     1921 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     1981 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2041 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2101 cgccaggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2161 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2221 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2281 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2341 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2401 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     2461 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc
     2521 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     2581 ttatgaacaa gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg
     2641 tagcacttgc agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc
     2701 ttgatttctc g
//
LOCUS       DAZSNO010000295         2661 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZSNO010000295 DAZSNO010000000
VERSION     DAZSNO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.299"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            77..328
                     /locus_tag="ACHWN2_004202"
     CDS             77..328
                     /locus_tag="ACHWN2_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8933469.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            330..2426
                     /locus_tag="ACHWN2_004203"
     CDS             330..2426
                     /locus_tag="ACHWN2_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR8933470.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT          723 a          615 c          735 g          588 t
ORIGIN      
        1 tttggtatct taatctttat ccatacggat atccgaccgg atatccgcgc gggtaatttt
       61 gtaggttaaa ggaaagatga gacaaagaga agatagtgac cgcgattggc cttcgctgaa
      121 gattcagctg gcgctcaaag ataacgggtg gtcttccttc cgggagcttt cactccatgc
      181 tggttatgct caggattcac tgaaagcggt agttcgcaca ccaaacaaaa aatacgaacg
      241 cgtaatttct gacgctatag gtgtaccgcc agaaatcatc tggcccagcc ggtatcgtaa
      301 cgatggattt atcaggaagg cgtcgtgata tgcagtcgta cgttaccgta aatgacctgc
      361 tcggcgtacc aggaatgcca gccaccacaa aaggcatccg acaggcgtta cagcgttttt
      421 ctcgtgatct tggtgacgtt agtcgccgtc gggaaggcac aaaagccatt gagtaccaca
      481 ttgattgtct gcctgaaata acacgaaagg cactgcgtga acgctatgtg gaacagctgg
      541 tggctacaga gaacaatgtt tctgaagtga aagctgttac cagaaaaaca cgcaatcctg
      601 acgctgtcca ggcaatcgaa gcatatcgcg gttctccaca actgatggaa gaacgcctga
      661 atgcgctgac cgaaaaccag cgctgggtat ctgaagcaag agctgcgctg gtggtggaag
      721 tgctgaagct ggaaagcgcc ggtaaccccg ggcgactgaa agccattaac tttcttgttg
      781 aaaaagcccg taaaggtgag ctgccggagc gcctgcaaca ggccgcagtt aacgccaatg
      841 caaaacgtgg cgctaatcgt actatcagcc gcgatccact ttaccagtgg gttctgaaat
      901 acaaccagtc gcagaacgcc gccgaacggc tgctgttact ggcaccggga aaacgcgacg
      961 aaataaaacc ggaagaaatc agctggctgc ctgaatttct ggcgcagtat cgccaggtga
     1021 acggcagacc catgtcagaa gcctacgagg attttgtcgc agaatggcag cgtcgccatg
     1081 cggacgaacc gtacatgctg gaggtgatgc cttcctatga tgttgtccgg tatgccatga
     1141 aaaaactgcc ggaagtggtg aaacaaaaag ggcgcgttac cggcagtgaa taccgtcagc
     1201 ttgaaggatt cacccgccgc gactggacag ctatgccggt gaattatgtc tggattggtg
     1261 acggtcacgg tatgaagctg aaatgtgcgc atccgatcca cgggcgtcca ttctcaccgg
     1321 aagtcacgtt tgtgatcgat ggtggaacac ggtttgttgt tggctggagt cttgaccttg
     1381 ccgaaaacgt gttcgccgtt gccggtgcga tacagcacgg cattcgcaac cacggaaaac
     1441 cgttcctgta ttactcggat aacggttccg gtgaaacggc tgacatgctg gacaaagagg
     1501 tcgtgggtat tctgccacgc ctggggatta aacaccctac cggtattgcc ggtaatccac
     1561 agggacgcgg cattattgaa cgcctgaacc ggacactgcc gatgcgtata gccaggaggt
     1621 atcgcaccta ttttggcaag ggtgctgacc gggaatcgtt gcgtgtactt aaccgtgatc
     1681 tgcgttcagc gtttaacgcc ctgcaacagg acaagcctct gaacgaccgc cagaaagcgg
     1741 caatgcgcga gctgccatcg tgggcggagc ttattgaggc tattcgtgaa ggcgtggaat
     1801 ggtacaacaa ccgcccgcat tctgagctgc cgatgaaacc gaacggacgg cattacagcc
     1861 cgacagagtt cagaaaaaaa cgccaggcag aagaagacac cgaaattgag tggctgagtg
     1921 atcttgagct gcgcgacatg ttccgcccaa tggtggaacg accggtaaga cgctgcgaaa
     1981 ttcagtggct gaacaatatt tactacgcgc cggagttgcg tgacgaacac ggtcgcaaag
     2041 tgcttatcag ttatgacatt catgacgccg agcgaattac ggtacgtcgc aaggacggca
     2101 gctttatctg tgaggcgata tggaacggca ataaacgcgc agcctttgct gtcagtgctg
     2161 aatatcacaa acaacagcag cgtattaaag gaatgcgcaa acgcgctgag gaaaaaattc
     2221 gtgatgctga agatgaaggt attcagattc tggaacacaa acaggctgaa ccctggctga
     2281 gtaatgttta tcgcccggtt ggtaatgtgg tggcagttca gcaaccggaa tatgaagaag
     2341 agcatgacga agaatttgaa cgcgatttcc ggctggggat gcaaaaactt tttgccatgc
     2401 aggaagaaga tgatccgctg gcctgaataa aaaaacagtc cgcgggaacg gactgttaaa
     2461 taaaggaaac aaagctgatt tgaaataacc gaggaaatac tatgactgat attgatgatg
     2521 tttgaggtgt actggcaata gcggacacta ccatttgttc tttttttaag cagccatctg
     2581 atgatatttt tccctgaagg ctgccgggga gatatttgag gtgtactggc aatagcggac
     2641 actaccattt gttctttttt t
//
LOCUS       DAZSNO010000296         2656 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZSNO010000296 DAZSNO010000000
VERSION     DAZSNO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.300"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..357
                     /locus_tag="ACHWN2_004204"
     CDS             <1..357
                     /locus_tag="ACHWN2_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8933471.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            619..981
                     /locus_tag="ACHWN2_004205"
     CDS             619..981
                     /locus_tag="ACHWN2_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR8933472.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            978..1907
                     /locus_tag="ACHWN2_004206"
     CDS             978..1907
                     /locus_tag="ACHWN2_004206"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR8933473.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            1904..>2656
                     /gene="gtrII"
                     /locus_tag="ACHWN2_004207"
     CDS             1904..>2656
                     /gene="gtrII"
                     /locus_tag="ACHWN2_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HFR8933474.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYN"
BASE COUNT          770 a          525 c          547 g          814 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa caaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tcttaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccgc ttgtaattca aaggattatc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat aggtgtgctg
      661 aacacactta tacactgggt ggtttttggt gtatgtatct atgccgcaca taccagtcag
      721 gctctggcaa acttcacagg tttcgtagtg gctgtgagct ttagcttctt cgcgaatgca
      781 agattcacat tcaaagcatt gactacagcg atgcgctaca tgttatacgt gggatttatg
      841 ggaatattga gtgtgattgt tggttgggca gctgataaat gctcacttcc tccaatagtc
      901 actcttatca ccttctccgc catcagtctg gtgtgcggtt tcgtctattc aaagttcatt
      961 gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc tgtcttcaat gaagaagaag
     1021 cgatacctgt tttctataaa acggtacgtg aattccaaga gttgaagcca tatgaagtag
     1081 aaattgtatt cataaatgac ggaagtaaag atgccacaga gtcaattatt aacgcgctgg
     1141 ctgtttcaga cccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag
     1201 ccttatttgc agggttagac catgcaagcg gcgatgctgt aattcctatt gatgtcgacc
     1261 tgcaagaccc aattgaggtt atccctcatc ttattgaaaa gtggcaggca ggtgctgaca
     1321 tggtgcttgc taaacgttca gaccgctcaa ctgatggacg actgaaacgt aagacagctg
     1381 agtggttcta taaattacac aacaaaataa gcaccccaaa gatcgaggaa aatgtcggag
     1441 attttcgact catgtctcgt gaggttgtgg agaacattaa actgttgcct gagcgcaatc
     1501 ttttcatgaa aggcatactg agctgggtgg gtggtccgac ggatgtcgtt gaatatgtac
     1561 gcgcagagcg tgttgctggc atctcaaaat ttaatggctg gaaattatgg aatctggcac
     1621 tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt atggacttat ataggcttgt
     1681 ttgttgcaag catttcattt ttatatggtg catggatgat tatagacacc cttgtctttg
     1741 gtaacccagt acgcgggtat ccctccctgc ttgtatcaat acttttcttg ggtggagtgc
     1801 aactgatcgg gattggtgtt ctcggagaat atataggtag aatctatatt gaagtaaaaa
     1861 atagacccaa atacatcata aaaaaatctc atcgaggtaa cccatgatta aaattaattt
     1921 atttaaaaat gcaaatctcc ttgccttcat atcatgtttt gcaatatcaa tttattgtta
     1981 ttgggggtgg ttgtacgatg gaacacttaa tattgatggc gagtttacaa ataattttta
     2041 tcaaacaatc acgcttgggc ggtggtttca cacttttttg cgacattact ttctccctga
     2101 gcctttttca ctttatataa caccattaat agccttatct tttatcatca tttcagcatt
     2161 tataatctgc agatcgctaa agctagaatc ttatgaatta ttgataggta tgttagtatt
     2221 tattaccttc cctcagatct cctatcaatt agagtttctt aaccaagctg atactgtggg
     2281 aattgctttt ctactggcag cgatatcagc aattattttt cactcgcaaa aaaataggat
     2341 tgtgatattt tctggtatag tactgtccat tctttcaatg gcaatctacc aaacattcgt
     2401 aacatatatt attgcattcg tcattgggtt gcagataaat tcgataatac gaaatgagaa
     2461 aaatattcgt gaatcttttt atagttcatg tttatctcta tccctcatag ctttatctac
     2521 cttaatttac ctgctattaa ccaaagctat caagcattat ttttcgcttg aatcgaacga
     2581 gtacatctca aattatatac aaaatgcaag cgatattaaa tggcttgtta aatcagccat
     2641 agataatata tataac
//
LOCUS       DAZSNO010000297         2634 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZSNO010000297 DAZSNO010000000
VERSION     DAZSNO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.301"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            193..1191
                     /locus_tag="ACHWN2_004208"
                     /pseudo
     CDS             193..1191
                     /locus_tag="ACHWN2_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(1278..1754)
                     /locus_tag="ACHWN2_004209"
     CDS             complement(1278..1754)
                     /locus_tag="ACHWN2_004209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933475.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(1747..2511)
                     /locus_tag="ACHWN2_004210"
     CDS             complement(1747..2511)
                     /locus_tag="ACHWN2_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8933476.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
BASE COUNT          762 a          548 c          489 g          835 t
ORIGIN      
        1 atggcctttt gaccgctaaa aaatggcact cggcacagta aaccccgcta tagttatgct
       61 atgcatttgt ctttaatgat atctcgtcgt tatattaaca ggctgaaatt atcattaatg
      121 cacatttcaa tattagcagg gatacctgag gagtttaaag tgaacatata tatcgggtag
      181 cttttcaaat taatcccttt gattataggc ttaatttgca tcgcgttagg tggctttgtg
      241 ctggaaagtt cagggcaacg tcacgggtca tgtgctgatt tctctggcgg ccatatgcct
      301 ggcattattc actaccgcat ttattatcat ttcgcaactc acgcgcggcg ttaatacgtt
      361 ttacaataca ttgttcccca ttattggcta tgcggggtca attatcacca tgatatgggg
      421 ttgggcactg ttagcaggta atgatgtgat ggcagacgag tttgttgccg gccatgttat
      481 ttttggcgtt ggtatgattg ccgcatgtgt atcgacggtg gcagcgtcgt ccggtcactt
      541 tctgctcatt cccaaaaatg cagcggggag caagagcgac ggaacaccgg tacaggctta
      601 ttcttcatta atcggtaatt gcctcattgc cgttcccgtt ttactcaccc tgctcggttt
      661 catttggtct attacgctgt tacatagtgc cgacataact ccgcattatg tcgccttggg
      721 ttaaccgcaa tctgtgcctg tctaattggc ctagtggcca caatcgtcca tcaaacacgt
      781 aatacgttct catccaaaga gcactggctg tggtgttatt gggttatttt tctcggctca
      841 gtcacggtgc tgcaggggat atacgtctta gtcagttccg atgcaagcgc ccgactggct
      901 cccggcatta ttctcatttg cctcggaatg atctgttaca gcatattctc aaaagtctgg
      961 ctactggcac tggtctggag acgtacctgt tcgttagcca acagaatacc gatgataccg
     1021 gtatttacct gtctgttttg cttgtttttg tcatcattcc tcgcggaaat cgcacaaact
     1081 gagctggact actttattcc atctcgggtt ctggttggat taggcgcagt atgttttaca
     1141 ttgttctcta ttgtttcaat attagaggcc ggttctgcga aaaaaaatta aaatttacat
     1201 aatcccagcg ttattcatat cttaacgctg gttttttacg aacttagtga aataatgtag
     1261 aatataaaat tataatttta tattggagca catgtgttaa tgatcaaccc cgggatacta
     1321 aaaatataga cattattaaa cttgcttagc ctcgcattgc ctttacctgt taaagatgca
     1381 tcgagaaaat tgagtctgaa gttctcatcc tttatctgat tttccagtcc cgaaactttg
     1441 gccttagcat tacctgagat ccgtccgtta ctatctattt tgtaggtaaa aaaatacgaa
     1501 tgggaaaatt tcctcggtgt atcttcatca gttaagccaa caatcgtaaa actaccgcta
     1561 ccatcttttt ttaaaatgac tgaaaggtta gcagagtagt ataaagaatg atctgtttta
     1621 gcatttcttg ttaacgtcga cgtacattca agagacgttg tgctaccatc acgagccttc
     1681 cagagagcaa aaaaacacac ctgtagcaat gtgaaaacaa gtattaatat aaaatacatt
     1741 ttcttattag tcatataata gcacctctga acaaattgca taagtttcgt cattagagga
     1801 ttttatacat cttgataaat accgctgccc ttccccaagc aaataatgca tactatttac
     1861 atcatatata aaatttgcac ctggcacaca gggtaaatgc tttttcgaaa tttcagcggc
     1921 ctctatttga gatttagcga ggccaatctc gttaagattc tctgaaagat ataaatcaca
     1981 ggtgtcaatc ttacctgatt taaccaatgg ataatccgtt atactgtagg tatctttaaa
     2041 agccacggca aacatttcaa taataacaat tactaccaca agcgaaatca aaatgcgaaa
     2101 aagaggaata ctccttttgc gctctacttt ttcaacaaca tcttcttgta aatgagaatc
     2161 atcataagat tgtttactta ttgttatgtt ttcactgatg taaaagccta ctttgggtat
     2221 cgtaagaatc acattttctt caacaccaat atttttaaaa cttttgcgta acagggatat
     2281 atattggttc aatgtgttgt ttgacagatc aaatgaaaat ctctccaaaa cgctatcaat
     2341 gattgagttc ctgtcaaccg ctccaccatt actttccagt aacactgcca aaatacaccc
     2401 cgccgtagct ggaaaattta tctcatcctg ggtatttaca ttcgagagcg agcatttgtt
     2461 cggatcaaaa ataatcgcgt cattgataat gaaaaaacat ctccgctcca tgcaggcaac
     2521 aacacaaaat aagattttgt cattttagcc tattcctggt ggaactgaca caagaaacta
     2581 aaatacaaat ataaccctga aaaagtcata taaaacttat atccacagaa taaa
//
LOCUS       DAZSNO010000298         2628 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZSNO010000298 DAZSNO010000000
VERSION     DAZSNO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.302"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            625..1224
                     /locus_tag="ACHWN2_004211"
     CDS             625..1224
                     /locus_tag="ACHWN2_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR8933477.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
     gene            1597..1980
                     /locus_tag="ACHWN2_004212"
     CDS             1597..1980
                     /locus_tag="ACHWN2_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR8933478.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            1977..2402
                     /locus_tag="ACHWN2_004213"
     CDS             1977..2402
                     /locus_tag="ACHWN2_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR8933479.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
BASE COUNT          652 a          637 c          662 g          677 t
ORIGIN      
        1 atggcttcac ccgtgttaat atcctggata actgtgtttg aaggatttct gttaataatc
       61 tgctcctgca actcacctga tgagtattag tggtaataca taatcttact cgtaaaagag
      121 tataccttat gtcattaaac agtcttccgg cgtgttgttc aatcctgtac ctctgttact
      181 tatctggtac gttgatatgt gtaatgctac gttaattgca atatctggtt atggctttat
      241 accttcagca tttaaaatat tttattaccg gagaaacagg gaagtggaaa gacttttggg
      301 ataatgtcag gccattctga cgttaatcac agtaccaaat aacgagaggg taatggaagt
      361 taaaacctgc gtaagttcct ctgttgttca ctggaaaaag tgattgccca cacgcgttat
      421 aaaggccttt ccgctgcagc ctggttaaca gagcttcagg cgttatatgc gctgtatggc
      481 tgttacgtat gctgttcttt gcctggttct gatacccgtg gaagtcgtta ccggaggaaa
      541 cacgttcacc ctactacctg agaacatctt cttcaaaaat tacggcaacg cttcttcctg
      601 tgttacagaa cacgaggaag aaacatgcaa acttataaca ccaatcccgc ttacgaaatg
      661 gctccccagc tcctggagca ctttaaccag catctggatt cattattcgg ggtctattcg
      721 aaactgttac cgttcagaat ggattttgct taccgaaaaa acaccctcag ctacagatgt
      781 gcctgcaggt acgccatgtg cgcggagatg ctgcaactga ttaacgaggt gggagagaaa
      841 ctggtcgggt atgcctgggt gatggaatat acagaacgta aagggcttca cattcatttt
      901 gtgggctatc tgaacggcca gagccacagg agttcgtatc tggtatccag attgatggga
      961 gatatctgga ggcgggttac cgagggtaat ggttactacc actggtgccg gtttaataaa
     1021 aattatccgg taaacattaa ccacgtcatt cactattcag atcacaaagc ggtgaatgat
     1081 ttacgctatg ccatcagcta cctggccaaa cgggaacaga aagaatgtgg catcatcctg
     1141 aaatgcagcg ggcttcctga aaaaagtaac cggggtcgac cacgactgga cagccccctg
     1201 ccggggatat gtgctctggt gtaaacccct gaacgacaag tacgggctgg cgctaatctt
     1261 tgctgtcggt tccggcggcc acttcagcgg cactgcgcat tttttacggc gtggtcattc
     1321 cgtatgaacc atacggagat ttaaccatga cacattcttt accattcctt ccggcaaccg
     1381 atacagcaca tcccccaatc tccatctctg cttactgact gttttttacg tcagtgggcc
     1441 attgtgtgac aaccacagtg ctgtaacagc aaccagcctg ctttacccaa actccagtcc
     1501 agattttaac tgaaccgcca ttcacaccct gaccacgctg cccgtaccgg gacggctgtt
     1561 gcctgcgtgt cgtctggcgg ctgaggaaca tatactatgt acgcaaaatc ttttctcgca
     1621 cttgatggca acggacgtct gaccggtgcc cgtaccgcac agactgcacc ttacgatagc
     1681 tacacctgcc acctttgcgg cagtgcactc agataccacc cgcaatacga cactgaacgg
     1741 ccctggtttg aacacgctga cgaagggctg acggagcacg gtcatgagtg cccttatgtc
     1801 aggccggaac gcagagaaat acggttgatt aaacgtctgc agcaattcgt cccggatacc
     1861 ttacccgtag tacgtaaagc cagctggcac tgcaggcaat gtcaccacga ttattatggg
     1921 gagcagtact gcacacactg ccagaccggg cgcttcagtg aggagggggg agcagaatga
     1981 acggtataaa aacggggctg gggatcacgc cgggtgagca cattatcagt gccgactctg
     2041 cgctcagcag aaatatccgg cagtgtttct gcctttcctg ccatggccgg ttgattctgc
     2101 agacagatgc tcagggggcg tggtttgagc atgatttaca tgccctgagt gagcaacaga
     2161 aggcggactg tgtggtgctg aatccggaga agtcccaccc ttacattgag gatatggcga
     2221 tgtttttatc gccattaccg gtcgtgctgg agtggcattg tgtgatgtgt gagcagtttt
     2281 ttcatgggaa gaagttctgt gaggcctgtg gtacggggat ttattgcaga gcggtatgta
     2341 cgaagtctgt ttacagctac cagccagact tgtttgagga ctgcggaggt gcgagtactt
     2401 aatccgaaat gcccgacctg ctcagggcgt tcagaggtat tgccaacctg tattttatct
     2461 ggtgtttcgc acccaccccg ttcgtgacac aaaggcgcga caccggatat ccgcagtcct
     2521 gcttacctga ataaaacccg gggtattgat tggtagtgtg gtgcggaatc gtaccgtgat
     2581 ctcgcattcg ccgaattcgc actgtggtgt caatcaacca tacgcgag
//
LOCUS       DAZSNO010000299         2625 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZSNO010000299 DAZSNO010000000
VERSION     DAZSNO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.303"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACHWN2_004214"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACHWN2_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR8933480.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..>2625)
                     /locus_tag="ACHWN2_004215"
     CDS             complement(1899..>2625)
                     /locus_tag="ACHWN2_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933481.1"
                     /translation="NIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNIDTDL
                     LVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSCNLLS
                     LCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQSPADK
                     YDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIVTEKV
                     SSGRTAGGHHHRHTGYTWSVSVN"
BASE COUNT          679 a          690 c          624 g          632 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaggcaac ctccggttcg aagaggctgg ccatctggcg
       61 accttctgct tcccttgcag caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgtc tgatatttca gtgagagttt
      181 tactgccgga tacggtccgg gcagagtgac ataacaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctggcgctc catctcctta ccggtcgata ccccgtcacg
      301 cacggggtca gcaccgtagg aatactgcag actggctttc aggtgctctt tctcaccaat
      361 ttcaccggca gcaaactctg caatctgatg gctcggggag cggaaaaagg cacgggtatt
      421 cagcacatca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagcctcc gaacttacgg gcttccggca ggatctccac
      541 caggtccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggaaaccac
      661 cggcttcagg gaggcatgag tgtcggcatt tgacgagata aacagccagc cgtttttctg
      721 atcttcccgg acaccccgca tccagtcacg tatggtgaag ggttcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttcg attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcagatacg ccgcttccgc aaaaatggta cgtcctgatc cctgccagaa
     1021 cgggtcctct ttcgttccca tcgggatcag cgtgttcgcg acattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccaggc ggcacagcgg gcatccagcg gattcaggat
     1141 cttatcgatg gagggatcat agtaactttt aacaaactca cacgaacggt cataaatcac
     1201 caccatgtcg ccacgtttac gggcatagtt ggcaagacgt cgaataacct ctgacttacc
     1261 cgtcgacact gttccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 atcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtaatcagg caaataacca gtgccacaac
     1501 tgtagccaga acgaatgcgg accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctga gcagcgttca tccggaaggt tttgccgtaa tactggatct catagaccgg
     1621 ctgggatttt attaaatccc gcatgccttc cagtgacgta caccaccagt aaatacagcc
     1681 gttgataaac gtctgccagc ttatttttac ccataagacc agaccaatga ggatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatgcccg cctgcagtcc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccatgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacaca ggacgtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaaa tcgcccaggt gatattctcc gcctggtggc catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggaaac ttcagccagc
     2281 tttctgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt tgtcagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttgcaggc
     2581 ttctcagtaa attgattatt ctcaccaata tattcagcga tattg
//
LOCUS       DAZSNO010000300         2595 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZSNO010000300 DAZSNO010000000
VERSION     DAZSNO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.304"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..197
                     /locus_tag="ACHWN2_004216"
     CDS             <1..197
                     /locus_tag="ACHWN2_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR8933482.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="ACHWN2_004217"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="ACHWN2_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFR8933483.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="ACHWN2_004218"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="ACHWN2_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFR8933484.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASLGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="ACHWN2_004219"
     CDS             1821..2576
                     /locus_tag="ACHWN2_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFR8933485.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          623 a          613 c          663 g          696 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc tgggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctc
//
LOCUS       DAZSNO010000301         2588 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZSNO010000301 DAZSNO010000000
VERSION     DAZSNO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.305"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            23..424
                     /gene="spa15"
                     /locus_tag="ACHWN2_004220"
                     /gene_synonym="spaK"
     CDS             23..424
                     /gene="spa15"
                     /locus_tag="ACHWN2_004220"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR8933486.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            428..1720
                     /gene="spaL"
                     /locus_tag="ACHWN2_004221"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             428..1720
                     /gene="spaL"
                     /locus_tag="ACHWN2_004221"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR8933487.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            1801..2151
                     /gene="spa13"
                     /locus_tag="ACHWN2_004222"
                     /gene_synonym="spaM"
     CDS             1801..2151
                     /gene="spa13"
                     /locus_tag="ACHWN2_004222"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFR8933488.1"
                     /translation="MYRDVEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
     gene            2138..>2588
                     /gene="spa32"
                     /locus_tag="ACHWN2_004223"
     CDS             2138..>2588
                     /gene="spa32"
                     /locus_tag="ACHWN2_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFR8933489.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNR"
BASE COUNT          860 a          376 c          547 g          805 t
ORIGIN      
        1 gactatttag tgaggtttaa atatgagtaa cattaattta gttcaattag ttagagatag
       61 tcttttcacg attggttgtc ctccatcaat tattacagat ttagacagcc attcagcgat
      121 taccatttct ttggatagta tgcctgctat taatatagct cttgtcaatg agcaggttat
      181 gttatgggct aattttgatg caccaagtga tgtaaaactt cagtcatctg cttataacat
      241 attaaattta atgttaatga actttagtta tagcattaat gagctggtag agcttcatcg
      301 ttctgatgag tacttacagt taagagttgt tattaaagat gactacgttc acgatgggat
      361 tgtttttgca gagattctgc atgagtttta tcagaggatg gaaatcttaa atggggtctt
      421 ataatcaatg agctatacaa aattgctcac tcaattatct tttcctaata gaatctcggg
      481 gccaatcttg gaaacaagtc ttagcgatgt ttcgattggt gagatttgta acattcaggc
      541 tggaattgaa agtaatgaaa ttgttgcaag agctcaggtt gtaggatttc atgatgaaaa
      601 aacaatatta agcttgattg gaaattctcg tggactttca cggcaaacgt tgattaagcc
      661 cactgcccag tttcttcata cgcaagtcgg ccgtggatta ttgggagcag tagtcaatcc
      721 tttaggggag gttactgata agtttgctgt tacagataac agtgaaattc tttatcgacc
      781 tgtagataat gctcctccgc tatatagtga aagggctgca attgagaagc cttttttaac
      841 aggtattaag gttattgatt ctttactcac gtgtggtgaa ggacagcgaa tggggatttt
      901 tgcgtcagct ggttgtggca aaacttttct catgaatatg ctcattgaac atagtggtgc
      961 tgatatatat gttattgggt taattggtga gcgaggtcga gaggttactg aaacggttga
     1021 ttatttgaaa aactctgaga aaaaaagcag gtgtgtttta gtatatgcaa cttcggatta
     1081 ctcttcggtt gatcgttgta atgctgcata tatagccact gctatagccg aattttttag
     1141 gactgaagga cataaagtag cgctttttat tgattcatta acaaggtatg ccagagcatt
     1201 acgtgatgtg gccttagccg ctggagaatc acctgccaga agaggctatc cggtttcggt
     1261 ttttgatagc ttacccagac ttcttgaaag gccaggaaag ttaaaggcag gtggctctat
     1321 tactgcattt tacactgttc ttttggagga tgatgatttt gctgatccat tagctgaaga
     1381 ggtaagatcc attttagatg gacatatata tttgagcaga aatctagccc aaaaaggaca
     1441 atttcctgca attgattcct taaaaagtat aagcagggta tttacacagg ttgttgatga
     1501 aaaacatcgt attatggccg ctgcatttcg ggagctactt tctgaaatag aagagctaag
     1561 gacaattatt gactttggtg aatacaaacc gggggagaat gcctctcagg ataaaatcta
     1621 caacaaaata tctgttgttg agagttttct gaagcaagat tatcgactcg gttttactta
     1681 tgagcagaca atggagctta ttggtgaaac aattagataa ggttttaaaa atcaaagata
     1741 aatatcaacg gtctgttaaa ttaattgaag cacacatatt aacactaaaa aaaaaactct
     1801 ttgtatcgag atgtggaggc attagataaa aggattatat acttcctgca actggagaat
     1861 gacttagaac ctgtaggcgc tcagagtgtt agtcagcttt tcaatacaag acgtaagatc
     1921 gctatagtaa aaaagcatat tatccaatat cagtctgaac gaattcttct aaagggaaga
     1981 attgaagaaa ttcaaaaaga tattgatgag gcaaatgctt ctaaaagaaa actgcttcat
     2041 aaagagagta aaatatgtaa acggattggc ttgattaaaa gaaataactt tgccaaacag
     2101 ttgatacttg atgagttatc acaagaggat atgaagtatg gcattagata atataaacct
     2161 aaattttagt agtgacaaac agatagaaaa atgtgagaaa ctatcttcaa tagataatat
     2221 tgatagtctc gttttgaaga agaagaggaa ggttgaaatt cctgagtact ctttaattgc
     2281 atctaactat tttactattg ataagcactt tgaacataag catgataaag gagaaattta
     2341 tagtggcatt aaaaatgcgt tcgaacttag aaacgaacga gcgacatatt ctgatattcc
     2401 agaatcaatg gccattaaag aaaatatttt gataccagat caagatatca aagcaaggga
     2461 aaaaataaat atcggcgata tgagggggat cttttcatat aataagagtg gaaatgcaga
     2521 caagaacttc gaaagaagtc atacttcttc tgtaaaccct gataatctgc tagaatctga
     2581 taatagaa
//
LOCUS       DAZSNO010000302         2586 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZSNO010000302 DAZSNO010000000
VERSION     DAZSNO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.306"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            10..687
                     /gene="tnpA"
                     /locus_tag="ACHWN2_004224"
     CDS             10..687
                     /gene="tnpA"
                     /locus_tag="ACHWN2_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR8933490.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPSGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            687..1034
                     /gene="tnpB"
                     /locus_tag="ACHWN2_004225"
     CDS             687..1034
                     /gene="tnpB"
                     /locus_tag="ACHWN2_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8933491.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1054..>2586
                     /locus_tag="ACHWN2_004226"
     CDS             1054..>2586
                     /locus_tag="ACHWN2_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFR8933492.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSLRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKLLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPAAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWEALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVR"
BASE COUNT          623 a          713 c          723 g          527 t
ORIGIN      
        1 acaaaaataa tgtccatcat ttttaatgga cactatcgta tgaaacaccg gacctggatc
       61 actgaagctt tacgtcttca ctttgaagaa catttacccc gggttgtggc cgggcgtcgc
      121 ctgggtgtac caaaatcaac agtttgtagt atgtttgtgc gctttcggag agctggcctt
      181 tcgtggcctt tgccctcagg catgtcggag caggaacttg atgcctgcct ttacggacaa
      241 ttttccacgg taccagtcgt acgtcctgaa agcaccgtta tatccgaagc ccccgtggta
      301 aaaaaacgtc cccggcggcc caacttccct tatgagttta aaatcgcctt agtggagcag
      361 tcactgcagc ccggagcctg tgtggcgcag atcgcccggg aaaacggaat caacgataac
      421 ctgctcttca actggcgcca tcaataccgg aaaggtggcc tgctgccttc cggaaaaaat
      481 atgccggcac tgcttcccgt gacgttaacg ccggagccgg ataataaaat cccggctccg
      541 gcacaggaac cagagcagat aaatacaccg tccgacagtc tgtgttgtga gctggttctg
      601 ccggccggaa ctctcaggct taaaggtaaa ctgacgccgg cgttattaca gacacttatc
      661 cgcgaaataa aagggagcag ccactgatga tatctctccc tgcaggttcg cgtatctggc
      721 tggttgccgg tatcaccgat atgcgaaatg gctttaacgg cctggcctca aaagttcaga
      781 acgtcctgaa ggatgacccg ttctccggac acctgttcat cttccgcgga cgccggggtg
      841 accagataaa agtgttgtgg gctgacagtg acggactgtg cctcttcacc aaacgcctgg
      901 agcggggccg cttcgtctgg ccagtcaccc gtgacggcaa ggtgcacctt actccggctc
      961 agttatccat gcttcttgaa ggtatcaact ggaagcaccc gaaacgaacg gaacgcgctg
     1021 gaatccgcat ataacccgtt gtaaagtgag gatatggaca cctcacttgc tcatgagaat
     1081 gcccgcctgc gggcactgtt gcagacgcaa caggacacca tccgccagat ggccgaatat
     1141 aaccgcctgc tctcactgcg ggtggcggct tatgcttccg aaatcaaccg gctgaaggcg
     1201 ctggttgcaa aactgcagcg catgcagttc ggtaaaagct cagaaaaact tcgcgcaaaa
     1261 accgaacgac agatacagga agcacaggag cgaatcagcg cacttcagga agaaatggcg
     1321 gaaacgctgg gtgagcaata tgacccggta ctgccatccg ccctgcgcca gtcttcagcc
     1381 cgtaaactgt taccggcctc acttccccgt gaaacccggg tcatccggcc ggaagaggaa
     1441 tgctgtcctg cctgtggtgg tgaactcagt tctctgggat gtgatgtgtc agagcaactg
     1501 gagcttatca gcagcgcctt taaggttatc gaaacacaac gtccgaaact ggcctgttgc
     1561 cggtgcgacc atatcgtgca ggcaccagta ccttcaaaac ccattgcacg cagttatgcc
     1621 ggagcggggc ttcttgccca tgttgtcacc gggaaatatg cagaccatct gccgttatac
     1681 cgccagtcag aaatataccg tcgtcagggc gtggagctga gccgtgccac actggggcgc
     1741 tggacaggtg ctgttgctga actgctggag ccactgtatg acgtcctgcg ccagtatgtg
     1801 ctgatgcccg gtaaagtcca tgccgatgat atccccgtcc cggtccagga gccgggcagc
     1861 ggtaaaaccc ggacagcccg gctgtgggtc tacgtccgtg atgaccgtaa cgccggttca
     1921 cagatgcctg cggcggtctg gttcgcgtac agcccggacc ggaaaggcat ccatccacaa
     1981 aatcacctgg ccggttacag cggagtgctt caggccgatg cttacggtgg ctaccgggcg
     2041 ttatacgaat ccggcagaat aacggaagcc gcgtgtatgg cccatgcccg gagaaaaatc
     2101 cacgatgtgc atgcaagagc gccaaccgat atcaccacgg aagccctgca gcgtatcggt
     2161 gaactgtatg ccatcgaggc agaagtccgg ggatgttcag cagaacagcg tctggcagca
     2221 agaaaagcca gagctgcgcc actgatgcag tcactgtatg actggataca gactcagatg
     2281 aaaacactgt cgcgtcactc ggatacggca aaagcgttcg catacctgct gaaacagtgg
     2341 gaggccctga acgtgtactg cagtaatggc tgggtggaaa tcgacaacaa catcgcagag
     2401 aacgccttac ggggagtggc cgtaggccgg aaaaactggc tgttcgcggg ttccgacagc
     2461 ggtggtgaac atgcggcggt gttgtactcg ctgatcggca catgccgtct gaacaatgtg
     2521 gagccagaaa agtggctgcg ttacgtcatt gaacatatcc aggactggcc ggcaaaccgg
     2581 gtacgc
//
LOCUS       DAZSNO010000303         2585 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZSNO010000303 DAZSNO010000000
VERSION     DAZSNO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.307"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            439..582
                     /locus_tag="ACHWN2_004227"
     CDS             439..582
                     /locus_tag="ACHWN2_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR8933493.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            595..804
                     /gene="cspE"
                     /locus_tag="ACHWN2_004228"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             595..804
                     /gene="cspE"
                     /locus_tag="ACHWN2_004228"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR8933494.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            970..1779
                     /gene="rlmA"
                     /locus_tag="ACHWN2_004229"
     CDS             970..1779
                     /gene="rlmA"
                     /locus_tag="ACHWN2_004229"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR8933495.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1776..2342)
                     /gene="mntP"
                     /locus_tag="ACHWN2_004230"
     CDS             complement(1776..2342)
                     /gene="mntP"
                     /locus_tag="ACHWN2_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR8933496.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2339..2461)
                     /locus_tag="ACHWN2_004231"
     CDS             complement(2339..2461)
                     /locus_tag="ACHWN2_004231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933497.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     regulatory      complement(2474..2564)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
BASE COUNT          677 a          615 c          621 g          672 t
ORIGIN      
        1 gtttctctct tgattttttt gctcactcat caatttttct tattttaaat ttacaatccc
       61 tttggggatt gacttctctt tagggtaatt gatagccgtt aactgactgt tttatgagaa
      121 aaagtgatat aactttttat tcattgcata gcagaaaatg tgatattgca cgcactatgt
      181 aataacttct ccctctggcc tggaacaact gaacttattg aactatgtta gaaactacgc
      241 cagtttaagt atctgcctga actggcaagg ttaagcacaa tgatatatcg gcgcgtattc
      301 cgttgcataa gtgtgcaaaa aaagtggaag acgtatcgag atttgtgcgt ctgatcgaga
      361 tatgtttaaa aatggcttgc cataattaac gttgtatgtg ataaaagatt tcgggttaaa
      421 cgaggtacag ttctgtttat gtgtggcatt ttcagtaaag aagtcctgag taaacacgtt
      481 gacgttgaat accgcttctc tgccgagcct tatattggtg cctcatgcag taatgtgtca
      541 gttttatcta tgttatgcct gcgggcgaag aaaacaatct aaggaatttt tcaaatggca
      601 aagattaaag gtcaggttaa gtggttcaac gagtctaaag gttttggctt cattactccg
      661 gctgatggca gcaaagatgt gttcgtacac ttctccgcta tccagggtaa tggcttcaaa
      721 actctggctg aaggtcagaa cgttgagttc gaaattcagg acggccagaa aggtccggca
      781 gctgttaacg taacagctat ctgatcgaat ccactgatct gaagtgtgaa tacgcttcaa
      841 tctcgctata aagcctcgtc gaatgcgagg ctttttacta tgctttatct tcgctcctgg
      901 cgttcggata tttgcccgcc gcgtgattcg cgttacactt gcggccttta gtatcctgcc
      961 ggagttgtca tgtcgttttc ctgtccactt tgccatcagc ctctttcgcg tgaaaaaaac
     1021 agctatatct gtccccaacg acatcagttt gatatggcga aagaagggta tgtcaatctg
     1081 ctgcccgttc agcataaacg gtctcgtgat ccgggcgaca gcgcggaaat gatgcaagca
     1141 cgccgcgcat tcttagatgc cggacattat cagccgctgc gtgatgcaat tgtcgcccaa
     1201 ctgcgggaac ggcttgatga aaaggccacg gcggtgctgg atattggctg tggtgaaggg
     1261 tattacacac acgcatttgc cgatgcgttg cccgaaatca ccacgtttgg cctggatgtc
     1321 tcgaaggtag cgataaaagc ggcggcgaaa cgctatccgc aggtcactct ttgtgtcgct
     1381 tccagccacc gtttgccgtt ttccgatacc agtatggacg ccataatacg tatttacgcg
     1441 ccgtgtaaag cagaagaatt agcacgagta gtgaagcccg gcggctgggt cattactgcc
     1501 acgccgggac cgcgacattt gatggagctg aaggggctga tttacaatga agtacatctt
     1561 catgcacctc atgcagaaca actggaaggt tttacattac agcagagtgc ggagttgtgt
     1621 tatccgatgc gtcttcgcgg tgatgaagcc gtcgcattat tgcagatgac gccgtttgcc
     1681 tggcgtgcga agccagaagt ctggcaaaca ctggcagcaa aagaagtgtt cgactgccag
     1741 acggacttta atattcacct ctggcagcgt tcttattaac cgtggaagtg cgtccagagg
     1801 atctggacgc cgatgccgat cagcaccagc ccgccgagaa tttccgcttt tttcccaata
     1861 attgagccga taaagcgacc aaccatcatc cctaatgtcg acataatcaa ggttgcacaa
     1921 ccaatggcca atgcggtcgc gataatgttg acctgaagga aagcaagacc aacacccaca
     1981 gccatggcat ccaggctggt ggcaatcgcg gtggttacca gtagccagaa accgtgccga
     2041 cggcgcggct cttcatcttc atcatctgcg ccacgaaaac cctcaataat cattcgcccg
     2101 ccgaggaata tcagcagcac aaacgcaatc cagtgattcc attcaaggac aaaccggctg
     2161 gctaacatgc ccattcccca gccgatcagc ggcgtcaggg tttcgacggc accaaaaata
     2221 aggccggttc gcaatgcttc agaaaatttc ggtttatgga ggggggcacc tttaccgatt
     2281 gatgcagcaa atgcatccat cgacatacca aacgcaagaa gaacagtagc agtgatattc
     2341 atgacaacgt cctgaccggg gtatccataa aacacatcat cgcccccagc aaacatgcat
     2401 acatgaacaa caagccgcga tgggttttcg cgttcatgca ggcatagcga ttgatgtgca
     2461 tatggtctcg cctgactgaa agtcttcagt ccgtacgtgc cacgttttgc aacgagtatg
     2521 ttgacacgta catttccgga atctggaaat cggctactcc ccaaatgagg gcgcaacctt
     2581 aacat
//
LOCUS       DAZSNO010000304         2578 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZSNO010000304 DAZSNO010000000
VERSION     DAZSNO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.308"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(384..1163)
                     /gene="ipaJ"
                     /locus_tag="ACHWN2_004232"
     CDS             complement(384..1163)
                     /gene="ipaJ"
                     /locus_tag="ACHWN2_004232"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR8933498.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1360..2169)
                     /locus_tag="ACHWN2_004233"
                     /pseudo
     CDS             complement(1360..2169)
                     /locus_tag="ACHWN2_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2516..>2578
                     /locus_tag="ACHWN2_004234"
     CDS             2516..>2578
                     /locus_tag="ACHWN2_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933499.1"
                     /translation="MVTFETVMEIKILHKQGMSSR"
BASE COUNT          780 a          636 c          409 g          753 t
ORIGIN      
        1 aaccccaaaa gttatgcttg gtcaaagagt aacttataat atccataccc ctcatagggc
       61 ttacagttta aattgcgggt ataaaataca tctattttca aataaaataa agcatactcc
      121 aattacaacg agtgactaaa ataaaaatat catccaagtc aatcactgtt tgctctgcaa
      181 aatatgtcag ccttctcaaa cttaccagca cttaaatatt taagcacaca atcattcctt
      241 gagggagttc acttatttca agagaaacat ttcgtcttcc cacacaacta atccgcccaa
      301 tatagctaaa tttgtactca gtaatatcat ttgttatgcc cccccaaaat attcaagctc
      361 cttcacagat aaaaaacgac aatttacaaa gcctcattag ttataactat ggaaatacct
      421 attttagtat atcctataaa gttagaatta cttcttcttg cacctaattc catagttgaa
      481 aaacaagaat aattttctcc tacagctgga tccatatagc ttccatcagg tctacacaac
      541 acccagtgga ggccgacagg aactccaata aaggatacag caacagcttc caaaactctt
      601 tggttagaag ataacacatc tcgttgatgt actatattac atcccatgcc aattaataag
      661 tcccttgcat cgggataaat aaaactaatt acttgcgaaa aaatgttact ttcttcaacg
      721 acataagcat tcaatccaag aagcctcgta gccatgacta tcttatcagg catagaataa
      781 ccggcatccg cgatattatc tttgtgtatt ctcggattat aattaccact agttattaag
      841 tataaatccc gctcacaacg attatccaaa tcaagagaac tcttccttgt catttcggac
      901 atagaccctt tgtattgtgg tattttatca acccctaatt cctttgcagc acagagtaaa
      961 ctggcagctc cacaagaatt gtcatacatc tgcttcctcg ccaaaaaaca accttgttcg
     1021 tttactctat tctcatttac cctcacatca gtctgatgag atatcatata ttctcttggt
     1081 atagccccct gcaataaaac cccatataac ataacacctg tatgaataca tccccgtttg
     1141 caaggcttcc gttgttccga catcgacgag taattgcagc acagtattgc atccagcaat
     1201 gcgaactact atgagaatta gctgaccggg tccttattcc ccgaacgaac tttaaatcca
     1261 aaacctcata ctcttttccg tcgtcatcac gtccgtcacc ataacaacct ttcagcatcc
     1321 acccttttat taagagttaa tgtccatcag catcagcaat taataccgcc tgatactgac
     1381 atatttttgt cgtatttacg tccataatcg gttattgtga tacaggtcac accagcttat
     1441 ttgctattgt tacaataacc accttggttg agagctgttt tttcaacttt gttatcaact
     1501 cccccaactc cttggtcagt aaggctgccg accgagcaca gtgaatagat agtatccgca
     1561 ggtaccacca tgcttactta atactcagaa ggcgaagtgc tctccaatta actctgccgc
     1621 actatgctta taagctgctt ttcaatgccg tctatcaaca tgtccagttc attcaatctg
     1681 ttgtactggt aatccaacgc agtaatgata tatgcaggta ttctttcatc cactccagta
     1741 acgtttcatg caaagcatta atttacgcgc tacggaactt caccagttga ctgagcaaac
     1801 gatgtgggac cagtactgcc tgcagttctt ctattgttac agcgatttct ttaccaaaca
     1861 gctgaaccac catcaagata aagatagcct gaacattcat cgcaccattt tgttaccaat
     1921 ggcaaaagat ttaacgaacc gtgcctgcat caggcggact ttttatccct gctttgttag
     1981 ctccctaacc ttgtacagag agcctccata agctcccata tcaagcagac atggttcgca
     2041 gttactgaca tacaccagaa aactctgaca gcagactcac ttatcctcca cttcactcat
     2101 atccttattg gtgagatgaa cctgaatgac atgttttgcg acataaacgc caaccagtgt
     2161 atatttcacc tgtagagatt ctacaatctg tagcgttatg catctaatgt taggctctat
     2221 gatgtcagaa atctgcgaga ctctacacct tctgcattta cctttattta tcaccatgct
     2281 cctcagttaa cggagaacgt gttcattgca ttttgtcact tacatttcgc ctttggtatc
     2341 ggggggatat aggttccaac gttccgcatg agaacctgaa ccggcttcac gccaccttga
     2401 taccagatgc cgcacaggta gtcaacaagt gtcacgaacg gtgcaatagt gatccacacc
     2461 caacgcctga aatcagatcc agggggtaat ctgctctcct gattcaggag agcttatggt
     2521 cacttttgag acagttatgg aaattaaaat cctgcacaag cagggaatga gtagccgg
//
LOCUS       DAZSNO010000305         2533 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZSNO010000305 DAZSNO010000000
VERSION     DAZSNO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.309"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="ACHWN2_004235"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="ACHWN2_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR8933500.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHWN2_004236"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHWN2_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR8933501.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHWN2_004237"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHWN2_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR8933502.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1993..>2533
                     /locus_tag="ACHWN2_004238"
     CDS             1993..>2533
                     /locus_tag="ACHWN2_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933503.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLD"
BASE COUNT          669 a          573 c          616 g          675 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccacgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ctttaagttt aactagggcg tcattattta ttaaatttta
     1981 tagacgctat atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg
     2041 aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg
     2101 ctttttgaaa acacactggt gtgttttatg cggttgtaca atgaactcag acgtaaagta
     2161 aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg
     2221 cagaatgata atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt
     2281 tattcagggg tttcaggagg tattacatat actatacaat atgttcaaga tgttgatatt
     2341 gttagagtgt ccttgccggg cagaggttca gagtctatca cagattttaa aggttattat
     2401 tggtatggct ttatggaata tattgaaaac attaatgcgt gtgatgatgt tttttctgag
     2461 tattgtttag ataatgaaaa tatgagtatc cagccagaac agataaatat gccgggaata
     2521 tctgatttag ata
//
LOCUS       DAZSNO010000306         2492 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZSNO010000306 DAZSNO010000000
VERSION     DAZSNO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.310"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>2492
                     /locus_tag="ACHWN2_004239"
     CDS             <1..>2492
                     /locus_tag="ACHWN2_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR8933504.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQEQ"
BASE COUNT          730 a          578 c          602 g          582 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggagcaa tt
//
LOCUS       DAZSNO010000307         2478 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZSNO010000307 DAZSNO010000000
VERSION     DAZSNO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.311"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            81..2348
                     /gene="adiA"
                     /locus_tag="ACHWN2_004240"
     CDS             81..2348
                     /gene="adiA"
                     /locus_tag="ACHWN2_004240"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR8933505.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          627 a          678 c          609 g          564 t
ORIGIN      
        1 ttttcacgcg ctttacagcc cgaaaaggcc ggaagatact tgcccgcaac gaagattcct
       61 tcataaccgg gtaagcaatg atgaaagtat taattgttga aagcgagttt ctccatcaag
      121 acacctgggt cggtaacgcc gttgagcgtc tggcagatgc tttaagccag caaaatgtta
      181 ccgtgattaa atccacctcc tttgatgatg gttttgccat tctctcttca aacgaagcca
      241 ttgactgcct gatgttcagc tatcaaatgg agcatccgga cgaacatcaa aacgtcagac
      301 aattgatcgg taagcttcat gagcgccaac aaaacgtgcc ggtcttcctg ttgggcgatc
      361 gggaaaaagc cctcgccgca atggaccggg atctgctgga gcttgtcgat gaattcgcct
      421 ggattctgga agataccgcc gactttatcg ccggacgcgc cgttgccgcg atgacccgct
      481 accgccagca gctgttgccg ccactgttca gcgcgctgat gaaatatagt gacatccatg
      541 aatattcctg ggcagcgcca ggccaccagg gcggcgttgg ttttaccaaa acacccgccg
      601 gacgtttcta ccatgactac tatggtgaaa atctgttccg taccgacatg ggcatcgaac
      661 gaacttccct cggttctttg cttgaccata ctggcgcatt tggcgaaagc gaaaaatatg
      721 ccgcacgcgt atttggtgcc gatcgctcct ggtcggtagt cgtcggtact tccggctcta
      781 accgcaccat catgcaggct tgcatgaccg ataacgatgt cgtggtcgtt gaccgtaact
      841 gccataaatc catcgaacaa ggtttgatgc tgacaggcgc gaaaccggtc tatatggtgc
      901 caagccgcaa ccgctacggc attatcgggc caatctatcc gcaggaaatg caacctgaaa
      961 ccttgcaaaa gaaaatcagt gaaagcccgc tgaccaaaga caaagccggg caaaaaccgt
     1021 cttactgcgt ggtaaccaac tgcacctatg acggcgtgtg ttataacgct aaagaagcgc
     1081 aggatctgct ggaaaaaacc tccgatcgtc tgcactttga cgaagcctgg tacggctatg
     1141 cacgtttcaa cccgatctat gccgatcact atgccatgcg cggcgaacct ggcgatcaca
     1201 acggtcctac cgttttcgcc acccactcca tccacaaact gctgaatgcg ctgtcacagg
     1261 cttcttatat tcatgtacgt gaaggtcgtg gggcgattaa cttctcccgc ttcaaccagg
     1321 cctacatgat gcatgccacc acctccccgc tgtatgccat ctgcgcatcc aacgacgtgg
     1381 cggtgtcgat gatggacggc aacagcggcc tgtcactgac acaggaagtg attgacgaag
     1441 cggttgattt ccgtcaggcg atggcgcggc tatataaaga gttcaccgct gacggtagct
     1501 ggttcttcaa accgtggaac aaagaagtcg tcaccgaccc acaaaccggc aaaacctatg
     1561 actttgctga cgcaccaacc aaactgctga ccaccgttca ggactgctgg gtaatgcatc
     1621 cgggcgaaag ctggcacggc ttcaaagata ttccggataa ctggagtatg ctcgacccaa
     1681 ttaaagtcag catccttgct ccgggaatgg gtgaagatgg tgaactggaa gaaaccggtg
     1741 ttccggcggc gctggtcact gcctggcttg gtcgccacgg cattgtgcct acccgcacca
     1801 ctgacttcca aattatgttc ctgttctcta tgggcgtaac ccgtgggaaa tgggggactc
     1861 tggttaacac cctttgctcc ttcaaacacc actatgacgc caacacaccg ctggcgcagg
     1921 tgatgccgga acttgttgaa caatatcctg acacttacgc gaacatgggg attcacgatc
     1981 tgggtgacac catgtttgcc tggctgaaag aaaacaaccc tggcgcacgg ttgaacgaag
     2041 cctattccgg cctgccggtg gcggaaatca ccccgcgtga agcgtacaac gcgattgtcg
     2101 acaacaatgt cgaactggta tccattgaaa atctgccagg acgcatcgcg gcaaactcag
     2161 ttatcccgta tccgccagga atcccgatgc tgctgtctgg tgaaaacttc ggcgataaaa
     2221 acagtccgca agtaagttat ttacgctcgc tgcaatcctg ggaccaccat ttccctagat
     2281 ttgaacacga aactgaaggg actgaaatta ttgacggtat ttaccacgtt atgtgcgtga
     2341 aagcgtaacc actattccgc tgaaggcgta attgtttaaa taacattacg ccgcctggcc
     2401 ttaggccttt tgagtatggc aacgttttca taaaaattgc tgcaaacaaa aatgtcatac
     2461 tttttgcgcg gccccacc
//
LOCUS       DAZSNO010000308         2454 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZSNO010000308 DAZSNO010000000
VERSION     DAZSNO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.312"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..673)
                     /gene="chbR"
                     /locus_tag="ACHWN2_004241"
                     /pseudo
     CDS             complement(1..673)
                     /gene="chbR"
                     /locus_tag="ACHWN2_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(681..1031)
                     /gene="chbA"
                     /locus_tag="ACHWN2_004242"
     CDS             complement(681..1031)
                     /gene="chbA"
                     /locus_tag="ACHWN2_004242"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR8933506.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1090..2438)
                     /gene="chbC"
                     /locus_tag="ACHWN2_004243"
                     /pseudo
     CDS             complement(1090..2438)
                     /gene="chbC"
                     /locus_tag="ACHWN2_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
BASE COUNT          688 a          632 c          528 g          606 t
ORIGIN      
        1 tctcattaat aatctgcatt ggtgttttgc cataatatcg ttgagtcgct cgcgtcaaat
       61 attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca ctaaactgct
      121 ctttatcatg catcttttct accgtacttt tcagccacta cggtacatca tcaatcacct
      181 gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc tcaacaaact
      241 cttccagccc tgtttcgcgg aaatttaatg aagaaatcac tgtttccaca taggtgagaa
      301 aagcgttatt ggtccggtat acctgcgaag caacaaagca ataaggcaac aacggcaggt
      361 aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa tatgcgcgtg gcaccaaact
      421 cataaaaact ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc cgttccagta
      481 acacgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga gtaaattcat
      541 aatagtcgtg ctgatgcagt ccgctgatac tctcagtttt gttatagata aacacatgga
      601 agtttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc gcgttaatca
      661 ctggctgcat catcgactcc ttatgccttc agtttttcat gaagctcaat taattcagta
      721 atcagttcac gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac cagcaccaga
      781 ctcactttca tcttaccttc gcccgcatcg ccttcaatca gtttcgtctg taccagatgc
      841 gcttcattca atgccatccg tgactgatcc atcatggctt tcgctgcggc aaaatcgccc
      901 tgcttcgcct gtttcaatgc ggcatacgcc aggctacgcg cttgtccaga gttgatgata
      961 agccccatca ccacttcttc cagctcttca gcttctgttt gcgtatcagg aatattatcg
     1021 agatccatca tacatcgttc ctctctcttc ttaccggcaa gattacccgt accggcatcg
     1081 attaaaattt caggggttag cgatatcttc ttcgctctct tctttatcaa tcgcattttg
     1141 tgctttgtta gccaccacaa caaagggcag ataaattaac gttgcgatgc caaggttgaa
     1201 gagtgcgacc agcaatgcgg cgacgctacc gttggtgtta aagaaggctc ccagaccggt
     1261 tggcatggtc cacggtgcaa tattggtcac cggaggaatg atgcccatgt agtacgctgc
     1321 gagggtgatt gccgccagaa tcggttgtac cagtacaaac gggataaaca tcaccgggtt
     1381 catgataatt ggcagaccaa acagaatcgg ttcattaatc tggaagatgc cggacggcag
     1441 cgccagtttt gccacctgac gataatcagc acggcgagag gcgataaaga tagccaggat
     1501 caggcctaaa gtcgcaccac tgcccccaag gaaaataaag gagtccagca tcggcttggc
     1561 ccagatatgg aaggtcttac cggctgccag cgccgcttcg acggaaccat attgctgata
     1621 ggtcgcgata ttttccagcg cccacggcgt cataatgccg ttgtccagtg cggtcagcgc
     1681 cagcgcgcca tgaataccga agaaccagag cagtggaaca aagatcacat aggcccagcc
     1741 caccacgcta cccaacgatg ccagtggggt tgagatggta tccataatga tctgatggaa
     1801 gttggtgccc caggtattca acgcccaggc aataatcccc atcacggaaa gaataataaa
     1861 gccgggaatt aatgccgaga aagaacgcga tactgaagca ggtacgctgt cgggcagttt
     1921 aatgacccaa ttgcggcgga caataaaggt aaacatttct gccaccacca ggccaataat
     1981 aatcccggag atgatattcg ccccacctaa ccagtttgcg ccaaccgcat aggcctcacc
     2041 gacactatat ggggtgacgg tcataaatgc tgcaacggat aacaacccag ccgccagcgc
     2101 atcgacttta cgctcttctg ccagcgccat gccaataaag aacggtgcca ttaaagacat
     2161 tattcctaat gttccgttat atacgttgcc gccaatacct ttcagaccat taagtgtttc
     2221 aatggttgag gcgtcgaggc gaatacctaa ggaataaaaa acgacccctc cccaaagctt
     2281 agaaaaacgt tgttaattaa tacaaacatg gcccccgcaa gggttaacgg cattaagcga
     2341 ataaagccat ttttgattgc attaacgtgt ggctgctttc ctattttaac tgcaaaaggg
     2401 aggagtacct tttcaagcga tgcaataaca ttactcatag aaaaataccc ttaa
//
LOCUS       DAZSNO010000309         2442 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZSNO010000309 DAZSNO010000000
VERSION     DAZSNO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.313"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..568
                     /locus_tag="ACHWN2_004244"
     CDS             <1..568
                     /locus_tag="ACHWN2_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8933507.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            526..1203
                     /gene="bglJ"
                     /locus_tag="ACHWN2_004245"
     CDS             526..1203
                     /gene="bglJ"
                     /locus_tag="ACHWN2_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR8933508.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1241..2029)
                     /gene="fhuF"
                     /locus_tag="ACHWN2_004246"
     CDS             complement(1241..2029)
                     /gene="fhuF"
                     /locus_tag="ACHWN2_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR8933509.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2170..2406
                     /locus_tag="ACHWN2_004247"
     CDS             2170..2406
                     /locus_tag="ACHWN2_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR8933510.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          629 a          534 c          627 g          652 t
ORIGIN      
        1 attagtcatt gctgatttag ccggtgaaag tgaagatcca cgttctgttt gtgaacatta
       61 ttattcttta atctcacaat atcgggaaat tcactgggtt ttcatggtgt cacgctcctg
      121 gtattcccag gctgtagaac tgctcatgtg ccctacggcg acgttattgt ctgatgttga
      181 acccattgag aatctggtca agaccgtacg ttccggcaat acgcacgcag agcgtatcag
      241 cgccatgctg acctctccgg caatgactga aactcatgat tttagctatc gctccgtcat
      301 tctcactctt tcagagcgca aggtactgcg gctattaggt aaaggatggg gcatcaacca
      361 gatagcttca ttgcttaaga aaagtaataa aactatcagc gcccaaaaaa acagtgcgat
      421 gcgtcgactg gcaattcaca gcaacgctga aatgtatgca tggataaata gcgcgcaggg
      481 tgcaagagaa cttaacttgc cttctgttta tggagatgcc gcagaatgga acacagccga
      541 attaagaaga gaaatgtcgc actcatagaa aaatgcgtca tgagtagtat cggtattgag
      601 agtttattca gaaagtttgc gggcaaccct tataagctcc atacctatac cagtcaggag
      661 tcatttcagg atgccatgtc gcggatctcg tttgcggcgg tcattttttc tttttctgcc
      721 atgagaagtg agcgcagaga gggattatct tgcctgactg agctggcgat taagtttccg
      781 cgtacccggc gtttagttat tgtggatgat gatattgaag ctcggctgat tggttcattg
      841 tcgccatcac cgctggacgg tgtattaagt aaagcgtcaa cgctggagat ttttcatcag
      901 gaactctttt tgtcattaaa tggtgtacgt caggcgaccg accgactgaa caatcagtgg
      961 tacattaacc aaagccggac gttaagcccg acggagagag aaatattgcg ctttatgtcg
     1021 cgtggctact caatgacaca aattgccgag cagcttaaac gtaatatcaa aacgatccga
     1081 gcgcataaat ttaatgtgat gtcgaaactg ggcgtcagtt ctgatgcagg gttgttggag
     1141 gccgaagata ttctgttatg tatgcggcat tgcgaagcaa gtaatgtgtt gcatccctat
     1201 taatccgcat gatgccgggt ttacttcccc ggcagtgctt tcatttcagc gtacaatagc
     1261 cacattgctg cacatccggt aagcgataac gctggcagca agtgcggcgc accagcaggc
     1321 cgtcgcgcag taccacggta cgccagagtg gattatcttc accgttcgtg agcgttttct
     1381 caaaaaagag ggcatggcgc agcgattcaa cagtagcctc gccgagcagt tgcttcatct
     1441 cagtgagata ccagttgatc aaataaccgg tattactcca gataagtttg ccgttgatct
     1501 ctccggtcgc ttctagtgct tgcacaaccg gaaccagcgc ctggctgatt aacgtttcca
     1561 ttcgctgctg cggcgaatgt ggtgttgcgt ttttatcttc acacacatcg acccagaaac
     1621 aggcgacgcg tccggtttcg tgaaactcag catggaaatg ttccggcgac acatctaatg
     1681 ctttttcctg cgtcagtagc gccagcatta atggtggcac catcaggccg atataccatt
     1741 gtgcccatag tgagatcagc ggtttgttct cgcggatcat cgttggttgg ttgcgataga
     1801 tatgatcgga atagaccgcc agtagagaac ttagcgcatt cggtgatgac cattgcgcca
     1861 gcgtcatggc gttaagcgga gcgggttcat ccaggcggat aaactccagc aaatgttcac
     1921 gatgtttggc gatcatcgcc cgtacggctt gtgcaagccc agcatcctgc ggctggagat
     1981 gcgttcgcca gatgacatct tcatagagcg gtgcggaacg ataggccata atcgtgatag
     2041 taatctaaat gataatgatt gctaatccta gcgataggtt tactcgatag caagggattt
     2101 ctctggcttg caaatgataa aaattatcat atgatattgg ttttcattat caatgaaaga
     2161 gatgaaacca tgttgcaacg tacgctgggc agtggctggg gagtgttgct gccgggattg
     2221 ctgattgcag ggctgatgta tgcggattta tcgccggatc agtggcggat tgtcattctg
     2281 atgggattag tattgacgcc ggtgatgctg tatcacaaac agttgcggca ttacattttg
     2341 ctaccatcgt gcctggcgct tattgctggc atcatgctga tgataatgaa tttgaatcag
     2401 ggatgacaaa acagggaaga aacaagaaag gaagtaaaga ta
//
LOCUS       DAZSNO010000310         2364 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZSNO010000310 DAZSNO010000000
VERSION     DAZSNO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.314"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACHWN2_004248"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACHWN2_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR8933511.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="ACHWN2_004249"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="ACHWN2_004249"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR8933512.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="ACHWN2_004250"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="ACHWN2_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR8933513.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
BASE COUNT          532 a          609 c          554 g          669 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 attgccagcg aaacgtttct ctta
//
LOCUS       DAZSNO010000311         2359 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZSNO010000311 DAZSNO010000000
VERSION     DAZSNO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.315"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1224
                     /locus_tag="ACHWN2_004251"
     CDS             <1..1224
                     /locus_tag="ACHWN2_004251"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR8933514.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="ACHWN2_004252"
     CDS             1224..1958
                     /locus_tag="ACHWN2_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR8933515.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            complement(2175..>2359)
                     /locus_tag="ACHWN2_004253"
     CDS             complement(2175..>2359)
                     /locus_tag="ACHWN2_004253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933516.1"
                     /translation="PTTPYSFLDYIYVAHIVNMAFLNFVISSIQRINLAHSYNSLQKP
                     TIKKSPIFASKYLTKT"
BASE COUNT          616 a          469 c          591 g          683 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgattttttt
     2221 attgtaggct tttgaaggct attataactg tgcgctaaat taatgcgttg aatagaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggtt
//
LOCUS       DAZSNO010000312         2353 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZSNO010000312 DAZSNO010000000
VERSION     DAZSNO010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.316"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            77..178
                     /locus_tag="ACHWN2_004254"
                     /pseudo
     CDS             77..178
                     /locus_tag="ACHWN2_004254"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            complement(267..779)
                     /locus_tag="ACHWN2_004255"
     CDS             complement(267..779)
                     /locus_tag="ACHWN2_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR8933517.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            974..1738
                     /locus_tag="ACHWN2_004256"
     CDS             974..1738
                     /locus_tag="ACHWN2_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933518.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1689..>2353
                     /locus_tag="ACHWN2_004257"
     CDS             1689..>2353
                     /locus_tag="ACHWN2_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR8933519.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          664 a          529 c          593 g          567 t
ORIGIN      
        1 ccctgtgcca tcctcagccg aaacctccag cgcctccggc atgggcaatg atgccaccgt
       61 cgaactctct ccagttgctg gacgaaacgt tctcggtatc cgggacggaa tcatcagcga
      121 ccagacagca ttgagaatgc ttcaggagta catcaggatc caatgccttg gggggtagcg
      181 gtaattttac tcattatcct tcaaatcaga ttctgttgtc agaggaatgg gggaagctgg
      241 attcgcaaga gctttttatg ggcagtttag ctaaaaaaag gcaacacgaa gctaaaacta
      301 ccaacataaa gtgctgacca gctagcagct gaaatcagac tcactgtata cactttcact
      361 ggatgaagtt gcaacattcc tgccaccaaa ggagcaatgt aacgcagcac ggcgataaag
      421 cgcgaagtga aaagtattga cacagagtta ttttgcaact gaaggcgaac ccgttctatt
      481 gtattggaac ggctcgatat tatacatgca agttgaggga tatgcccaat tattatacca
      541 agatggtaat taacgatagt accgcaccat gcgccactca tgaccgtgat tccagcttcc
      601 catggtgaaa gagtcgtctt gctgactgta attacagtgg ccatcataat tgatgctggt
      661 ggcaatatcg ccgaaatgag taaagtggat tttgtgaacg ctattataaa aagaagcccc
      721 cataggcgat atggatgaag tacaaaatag ttcatcaaag catttatcca ctccatcaat
      781 gtctttcctt ctgtgaattt ttctttgcta tttaaccgtg atacgactta atttaggctt
      841 aaacacctta catttacaag gtactcctgt ggggggccct gccacggagc gtcgcaggag
      901 tgagtgatga ggaagaatta aagcaaccta ttgtgacagg cagcgataac agtaaatcac
      961 aggaagcata atcatggcaa aaccggactg ggaggccatc gaatcggcat accgggccgg
     1021 agtccttagc ctccgtgata taggcgagaa atacggcgtt acagaagggg ctatcaggaa
     1081 gagggccaaa aagcttgatt gggcacgcag tggcggtacg caggtttgca aaaatggtac
     1141 gcaaaaaagg aaagtgcgta ccagcagaaa gcctgccatt actggcctta cacaaaaaag
     1201 tacgcaacta aaaacagaat ctacaccgga tacgaaaccg atacgcggaa tgcgtaccga
     1261 tcccccgact aacccattcc aacccggtaa ccagcaggca ttaaaacacg gtggttatgc
     1321 ccgtcgcctt ctgctcaaag atgaggtgat agaggacgct aaagcgttga cgctcgagga
     1381 cgaattattt cgccttcgtg ctaacaacct tgtcgctgca gagaatattg gtcggtggtt
     1441 ggtgtcgctg gaagatgcta atggggacca ggaaaggaag atgctgattg aaaatatcag
     1501 cgccgccgag aaagcaatga tgcgcaatac agttcgtatt gagtccatcg ttggcacgct
     1561 tgcgacggta ggaaaaatat ttgctgatac agcctaccgc aaggccgcca ctgataaggt
     1621 gtctctggag gctgatcgtc ttcgccgtga tgcaggtatt gatgatggca atggagagcg
     1681 tgacctcaat gacttctact ctgacatcca aaccgacgct gaatccggct ttacgtagtt
     1741 tctggactat gcgggcacgt aacaaagtgc tttatggtgg tcggtcatcg tcaaaatcat
     1801 gggatgccgc tggcattgcc atatttctgt cgaataaata caccctgcgt ttttgttgtg
     1861 cccgtcagat ccagaataaa atcgaagagt cggtgtatac cctgctcaaa attcagatag
     1921 acaggtttgg tctgcggcac cgtttccgta ttctgaacaa caaaatcatt aaccgggtta
     1981 ctggctcgga atttgttttt tatggattat ggcgcaacat cgaagaaatt aagtcactgg
     2041 aggggatcga tatgttgtgg ctggaagaag cccacgcact gacggaatac cagtggaaaa
     2101 ttctggagcc aacgatccgt aaagagggtt cggaatgctg gttcatattc aaccccggac
     2161 ttgttactga tttcgtctgg cgcaacttcg ttgttgatcc gcccgaaggc actctcatcc
     2221 gcaaaattaa ctatgacgaa aatccgtttc tgtctgacac catgcttaag gttatcgacg
     2281 cggcgcgacg ccgtgatccg gatggtttta aacatgtgta tgagggcgtt ccggagtctg
     2341 atgatgatgc ggc
//
LOCUS       DAZSNO010000313         2325 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZSNO010000313 DAZSNO010000000
VERSION     DAZSNO010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.317"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            61..282
                     /gene="kilR"
                     /locus_tag="ACHWN2_004258"
     CDS             61..282
                     /gene="kilR"
                     /locus_tag="ACHWN2_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFR8933520.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            282..452
                     /locus_tag="ACHWN2_004259"
     CDS             282..452
                     /locus_tag="ACHWN2_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFR8933521.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="ACHWN2_004260"
     CDS             527..802
                     /locus_tag="ACHWN2_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933522.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="ACHWN2_004261"
     CDS             902..>2325
                     /locus_tag="ACHWN2_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR8933523.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          719 a          531 c          529 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatgttcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAZSNO010000314         2317 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZSNO010000314 DAZSNO010000000
VERSION     DAZSNO010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.318"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..681)
                     /locus_tag="ACHWN2_004262"
                     /pseudo
     CDS             complement(1..681)
                     /locus_tag="ACHWN2_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            867..1709
                     /locus_tag="ACHWN2_004263"
     CDS             867..1709
                     /locus_tag="ACHWN2_004263"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR8933524.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1712..>2317
                     /gene="capU"
                     /locus_tag="ACHWN2_004264"
     CDS             1712..>2317
                     /gene="capU"
                     /locus_tag="ACHWN2_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR8933525.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEF"
BASE COUNT          622 a          464 c          531 g          700 t
ORIGIN      
        1 gttacggcga actgtcgctc aagatgattc gggatagcaa cgtgctcatg accgccacgc
       61 ttataccggt gagtcggctg ctggcaactg accagcccca gctctttcat gagtctgcca
      121 gcaagccagc gccccatctg gtagcctctc tgggttgcca ttgtggcgat gcttcttgct
      181 ccggcagagc cgtggctgat gccatgcagt tcaagtacct ggctgcgtaa tacagcccgt
      241 ctgccgtctg gtttttcagg acggtttttc cagtatttgt agctgctgcg atggaccccg
      301 aacacatggc agagagtggc cacaggataa cgcgccctga gtttcccgat tatcgaaaac
      361 tgttcaggga gtctgacatc aagagcgcgg tagccttttt aatatttcat tttccatttc
      421 aatacgttgt agctttttcc tgagctcacg gatttcaatt tgttccgggg taatggggga
      481 ggcttttggt gtttttccct gccgctcatc acgtaattgt ttcacccatc gcgtcattgt
      541 ggaaaggccg acatccatag cgctggctgc atctgccacg gtgtatttct ggtcaacgac
      601 cagttgagcg gattcgcgtt taaactctgc gctgaaattt cttttttcat tatggcacct
      661 gtgttgttct gaggtgagca tatcacctct gttcaggtag ctaaatttag tgtgccacct
      721 caaggttctt agtgagccgt ttttaaaaga tatgataata tattattgtt tataaattgt
      781 tgatgtttga tttagtagta tataaagttc accaccttta ttaggctttc cttttatctc
      841 tacttactgg gtgccagaaa ctttaaattt taaacggagg gggcattttg tttaaagcaa
      901 atcatgttcc ggtattaatg tatcatcatg tcagccattg cccaggactg gttacacttt
      961 ctcccgagac attccggaaa caaatgaaat ggttggctga aaacaactgg aaaacactgt
     1021 catcagatga actggagttt ttttatcggg gcggaaaact tccccgtaaa agcgttatgt
     1081 tgacatttga tgatggatac cttgacaact ggtttcaggt atatccttta ttaaacgagt
     1141 ttaatctcaa ggcacatatt tttttaatta ccagttttat tggaaatggt ccggttcgtc
     1201 actctcctgg aaaggaatat tctcatcgcg attgcgaaca tcaaatagca accgggaatg
     1261 ctgataatgt tatgcttcgc tggtctgaag tgaacgaaat gttgcaatct ggtctggtcg
     1321 aatttcatgt tcatacacat actcatacac gttgggataa aaagttcact tcacgagaag
     1381 aacagtgtaa acatcttcgg caggacttat tgagcgggag agaatattta aaaaaaatga
     1441 caggaaaatg cagtaaacat ttgtgttggc cggaaggtta ttataacaag gattatattc
     1501 aggtcgctga agaacttgga ttctattatt tatatacaac agaaagaaga atgaatgctc
     1561 ctgctaaagg aactacacgg ataggaagaa taagcaccaa ggaacgggaa aattgcgtat
     1621 ggctgaaaag acgacttttt tattatacaa caccgttttt ttcatcgttg ctggcccttc
     1681 ataaggggcc aagattaccg gatgactaat aatgaatata ctatttacgg aatcatcacc
     1741 gaatataggt ggtcaggaat tacaggctgt tgctcaaatg aaggccctga agaaaatggg
     1801 gcattcagtt ctgcttgtct gcagggagaa cagcaaaatt gcttttgaag ccagtaagtt
     1861 tggaattgat atcacattcg cgttatttcg aaacagtctt cacattccta ctgtatggag
     1921 attactcgga atagttcata gttttcagcc cgatgcaatc gtttgtcaca gtggacatga
     1981 tagtaatatt gttggtttgg tacggttttt tacctggaaa catccattca gaattatcag
     2041 gcaaaagaca tatttgacac gaaaaacaaa atttttttca ataaatcatt tttgcgatga
     2101 ggtgattgtt cccggaacaa atatgaagac acatctggag caggaaggat gtcgaactcg
     2161 ggttactgtt gtgcctcctg gctttgattt ccaggaatta tacgttgatt cccgaaacag
     2221 tttgcctccg agtgttcttt cctggctggc gtcccgaaga ggatgccccg taattgctca
     2281 ggtaggaatg ttgcgcccgg aaaaagggca cgaattt
//
LOCUS       DAZSNO010000315         2282 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZSNO010000315 DAZSNO010000000
VERSION     DAZSNO010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.319"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(141..236)
                     /locus_tag="ACHWN2_004265"
                     /pseudo
     CDS             complement(141..236)
                     /locus_tag="ACHWN2_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(272..577)
                     /gene="ccdB"
                     /locus_tag="ACHWN2_004266"
     CDS             complement(272..577)
                     /gene="ccdB"
                     /locus_tag="ACHWN2_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFR8933526.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(579..797)
                     /gene="ccdA"
                     /locus_tag="ACHWN2_004267"
     CDS             complement(579..797)
                     /gene="ccdA"
                     /locus_tag="ACHWN2_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFR8933527.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(1333..2282)
                     /locus_tag="ACHWN2_004268"
                     /pseudo
     CDS             complement(1333..2282)
                     /locus_tag="ACHWN2_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          530 a          621 c          556 g          575 t
ORIGIN      
        1 aaaattggct gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg
       61 tattgttctt gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct
      121 cgtcagaacc gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg
      181 cgtacccggt ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc
      241 aacgccacga tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg
      301 cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga
      361 tcggcacact ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca
      421 ccgggtaaag ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca
      481 ccatccgtcg tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac
      541 gactctctct tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg
      601 gcaaaagagc cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca
      661 gcctgccagc gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg
      721 cctgagatat tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca
      781 gtaatacgct gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata
      841 tattaatggt acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt
      901 ttaatacaat tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg
      961 taagtaaatt acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt
     1021 ccggaagtgt aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg
     1081 cccacagaga atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg
     1141 ccgcgaagta ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt
     1201 tcaggctgat tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt
     1261 cctgttgtca ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac
     1321 ggcaggtatc actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg
     1381 ctcctccggc cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag
     1441 accattacgt tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat
     1501 cagtgaaccg gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc
     1561 gcagatattg ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg
     1621 ccggttcaga atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc
     1681 ctgctcttca agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca
     1741 ctgccgtatt ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc
     1801 gatttttctc agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa
     1861 gccgcgccct gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag
     1921 agatttgtac acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca
     1981 ggtctcaggg tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc
     2041 gcttctttca ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg
     2101 ggtatcaagg agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc
     2161 atggcgtagc agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc
     2221 cattgtactg acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag
     2281 gg
//
LOCUS       DAZSNO010000316         2279 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZSNO010000316 DAZSNO010000000
VERSION     DAZSNO010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.320"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..796
                     /locus_tag="ACHWN2_004269"
                     /pseudo
     CDS             1..796
                     /locus_tag="ACHWN2_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            911..1252
                     /locus_tag="ACHWN2_004270"
                     /pseudo
     CDS             911..1252
                     /locus_tag="ACHWN2_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1235..2072
                     /locus_tag="ACHWN2_004271"
                     /pseudo
     CDS             1235..2072
                     /locus_tag="ACHWN2_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2122..>2279)
                     /locus_tag="ACHWN2_004272"
     CDS             complement(2122..>2279)
                     /locus_tag="ACHWN2_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR8933528.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGT
                     FYTPQGS"
BASE COUNT          553 a          554 c          692 g          480 t
ORIGIN      
        1 gggccttgcc cgtagcagtt atttttatca ccgggctgca ctgaaagccg gtgataaata
       61 cgccacgata cgtaagatgc tgacagatat ctttaacagt aattaccagt gttatggcta
      121 tcgtcgcctg catgcgatgc tcaggcatga gggggggcgg ctatcagaaa aggttgtacg
      181 cagacttatg gtggaggaac agcttgtcgt cagtcgtaac cgtcgtcgtc gctacagctc
      241 atattgcgga gaaatcggac cggctccgga taaccttatc gccagagatt ttaaggcgga
      301 gcaacctaat cagaaatagc tgacagatat cacggagttc cagctccctg caggtaaagt
      361 ctggctgtca ccagtggtgg actgcttcga tggaaaagtt gtgagctggt ctctcagtac
      421 acgccctgat gctgaactgg tcaacactat gctggatagc gctgtcgaaa cgttaaatgc
      481 tggcgaacga ccggtgatac acagtgacag aggtgggcat tatcgctggc caggctggct
      541 ggaaagagtg aatgcagcag gtcttattcg ctcaatgtcc cgtaaaggat gttcacctga
      601 taatgctgca tgcgaaggct ttttcggcag actgaaaacg gaaatgtatt atgggcgtaa
      661 atggtcgggc atcacgccag aaaagttcat gcagcaagtg gatgcttaca tcagatggta
      721 taacgagcgg cgtataaaat tatcgctggg tgccgtcagc cccaaaatgt accgccaaca
      781 atgcgggctg gaatgataaa gcagtccagg aaatcgtccg catcccccct tcaccatttg
      841 gattgtccgc tccacccaac atgttgtttc cttaaggttc ctacaccaca aaggaaactc
      901 tcatgctgcg cagtggatgg ctgacgttgc cgacaggcgg gaacttcgcc agttcaggca
      961 gacaccggaa cagcgcttca cgcaggaaca ggagcatctg cagccgctgc tgggtacgga
     1021 cttcgatatc cgtcatgtct cctgggatgg ctatatcgag gttggcggga accgttacag
     1081 tgtgccggaa agcctgtgtg gtcagctggt atcaatacgg atctcgctgg atgatgaact
     1141 gcggatctac agtaatgagc aacaggtagc atcgcaccgt ctctgttcgg ctgcatacgg
     1201 ctggcagacc gtgccggagc atcacgctcc actctggcag caggcttcgg agcgcatggc
     1261 cgaatggctg ggggagatac agaaacgggt aatcactgtc tgtgaccggg aagcggatat
     1321 ctggcattac ctgtattata aagtcagcca cggacagcgt ggtgcgtgct gcacagaatc
     1381 gccggctgga agaggcaccc ggcaagctct tcgaactgcc ggaagtcctg gcaaccgcag
     1441 gaagccacac gctgaatgtg atgcaaaaag gcgggcgggc agcccgtcag gcccggatgt
     1501 tcatcagtta cagcgaagtc agcataaaaa atcccgacaa cagcggccag gcgctcccgc
     1561 tcacgtatgt ctgttgccgg gagcaggcag aggacggtgc ctgctggcat ctgctgacgt
     1621 caggaaaagc ggcgagtgcc gccgatgcac gacgtattgt cagccattac gagcgacgct
     1681 ggctgacaga ggaataccac aaggcgtgga aaagtggtgg tacatggaat cgctgcgaat
     1741 gcagacccgg gataaccttg agcgcatggt ggttatcctg gcgtttatag cggtacgggt
     1801 gctgggcctg cggcagggag gtgtcagtga agaaacacaa aatgacagct gtgagaaaat
     1861 actgacgccg acagagtgga aactactgtg ggttaagctg gaaggaaagc cgcttccggt
     1921 gcaggcaccg accctgaaat gggcctgggg cgatggcatg acagcaaacg cacaggtcgt
     1981 cccggttggg gcgtcatgtg ggatggctgg tcagacttca ggatatggtt gagggttacc
     2041 tggtgatgaa gtctcttgat caggagctct gatcaagaga cagcagctta ggggggtgcc
     2101 cctattaagt tgtcaagcat gttatgaccc ctgcggggta taaaaagtcc cgtcgcgcat
     2161 catggcgaac agaacgtcgc agcgtcgtct cgccagggcg ataagcgcct gattgtgtcg
     2221 ttttccctga ctcattttgc gggtgtagta agccctggag agcggatccc tgagcgcgg
//
LOCUS       DAZSNO010000317         2234 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZSNO010000317 DAZSNO010000000
VERSION     DAZSNO010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.321"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..214
                     /locus_tag="ACHWN2_004273"
     CDS             <1..214
                     /locus_tag="ACHWN2_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YopN family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFR8933529.1"
                     /translation="FQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFK
                     SESEREIAINILRELVTSAYKKELSR"
     gene            227..>2234
                     /gene="mxiA"
                     /locus_tag="ACHWN2_004274"
                     /gene_synonym="virH"
     CDS             227..>2234
                     /gene="mxiA"
                     /locus_tag="ACHWN2_004274"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR8933530.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVI"
BASE COUNT          683 a          303 c          460 g          788 t
ORIGIN      
        1 ttttcaatat ccaagtgaaa gtgagcaaat tcttacttct gttatagaag tatcacgagc
       61 cagtcatgag gattctgtag tgtatcaaac atatctatct tctgttaatg aaagtcctca
      121 tgatatattt aaaagtgaaa gtgaaagaga aattgcgatc aatattctac gagagcttgt
      181 cacaagtgca tacaagaaag agctttctag ataacaggag ataaaagtga tccagtcttt
      241 tcttaagcaa gtaagtacta agcctgaatt aattatatta gtgcttatgg ttatgatcat
      301 tgctatgttg atcataccat tacctacata ccttgttgat tttttaatag ggcttaatat
      361 tgtacttgct attctcgttt ttatgggatc attttatatt gaaagaatat tgagtttttc
      421 tacattccct tcggttttgt tgataacgac attatttcgt ctcgctttat caataagtac
      481 tagtcgacta attttagttg atgcggatgc gggtaaaatt attactacat ttggccagtt
      541 tgtcattggt gatagtctgg ctgtaggttt tgttattttt tcaatagtaa ctgttgtgca
      601 gttcattgtt ataacaaaag gatctgaaag ggttgcggag gtagctgctc gcttttctct
      661 tgacggtatg ccggggaaac aaatgagcat tgatgcggat ttaaaggctg gaattattga
      721 tgccgcagga gctaaagaaa gacgcagtat tctggaacgt gagagtcaat tgtatggttc
      781 atttgatgga gcaatgaagt tcattaaagg tgatgcaatt gctggcatca ttattatttt
      841 tgtcaattta ataggaggta tttctgttgg tatgagtcaa catggaatgt ccctatctgg
      901 tgcattatct acttatacca tactaactat tggtgacgga ttggtctctc agattcctgc
      961 tctgttaatt tctataagtg caggatttat cgttacaaga gttaatggtg atagcgataa
     1021 tatgggacgt aacataatgt cccagatatt tgggaatcct tttgttctca ttgttacatc
     1081 agctcttgcc ttggcgatag ggatgttgcc aggttttccg ttttttgttt ttttcctgat
     1141 agcagttact ttgacggctt tattctatta taaaaaggtc gtagaaaaag aaaaaagtct
     1201 gtctgagtct gattctagtg gctatactgg tacatttgat attgataata gccatgactc
     1261 atctttggca atgatagaaa atctggatgc tattagttca gaaaccgttc ctttaatttt
     1321 attatttgcc gaaaataaga taaatgcaaa tgatatggaa ggtcttattg aaaggataag
     1381 aagtcagttc tttattgatt atggtgtcag gcttccaact attttatata gaacaagcaa
     1441 tgagcttaag gtcgatgata ttgttttgtt aataaatgag gtgcgtgccg atagttttaa
     1501 tatatatttt gacaaagtgt gtattacaga tgaaaatgga gatatagatg ctctaggtat
     1561 tcctgttgtt tcaacttcat ataatgaacg tgttatttct tgggtagacg tttcatatac
     1621 agaaaatcta actaatattg atgctaaaat taaaagtgct caagatgaat tttatcacca
     1681 gttgtcacaa gctttattaa acaacataaa tgagattttt ggtatacaag aaacaaaaaa
     1741 tatgttagat cagtttgaaa atcggtatcc tgatctatta aaggaagtct tccgacatgt
     1801 gactatacag agaatttctg aggtattaca aagattgctt ggagaaaata tttctgttcg
     1861 caatttaaaa cttattatgg agtctttggc gctttgggct cccagagaaa aagatgtcat
     1921 aacattagtt gaacatgtcc gtgcatcact ttctaggtat atttgtagta aaatagctgt
     1981 ttctggtgag attaaagttg tgatgctttc cggatatatt gaggatgcaa taagaaaagg
     2041 gataaggcaa acctctggtg gctctttctt gaatatggat atagaggttt cggatgaggt
     2101 aatggaaact ttagcacatg ctttgagaga attgagaaat gcaaaaaaaa atttcgttct
     2161 tttggtatca gtagatatac gtaggtttgt taaaagactt atagataaca gatttaagag
     2221 tatactcgtt atat
//
LOCUS       DAZSNO010000318         2230 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZSNO010000318 DAZSNO010000000
VERSION     DAZSNO010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.322"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..222)
                     /locus_tag="ACHWN2_004275"
     CDS             complement(<1..222)
                     /locus_tag="ACHWN2_004275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933531.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHA"
     gene            651..2159
                     /gene="ltrA"
                     /locus_tag="ACHWN2_004276"
     CDS             651..2159
                     /gene="ltrA"
                     /locus_tag="ACHWN2_004276"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR8933532.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          572 a          548 c          657 g          453 t
ORIGIN      
        1 ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt aactcacgat
       61 gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca gtgacctgcg
      121 gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga tcactaagcc
      181 gagatgatcc tcaaggttaa gtactgaaag gctgaagaac atgaacccgt taatccgcct
      241 ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca ggaggaactg
      301 gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga aacaccgggg
      361 cagcagcgtc tatcacactt gcgttgctga cttctgccaa cttgcggcaa gcaaggataa
      421 agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac cggggaaggt
      481 cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg agcttccgta
      541 gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga atgtgtttgc
      601 gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa atgcaacgca
      661 agcttgccac atgggcagcc accgatccgt ccctacggat tcaacggctg ctgcgtctga
      721 taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca aagggggccc
      781 atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg gctgttgagc
      841 tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc gccagacggg
      901 tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg ttgcgggatc
      961 gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt gattttcata
     1021 cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc acggtgaaat
     1081 tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc gacctgtcca
     1141 gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc aggatcagtg
     1201 acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc gatgtcggtc
     1261 tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg ctattatcga
     1321 acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc gggaaagcca
     1381 gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg gcggtcagag
     1441 aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat tttgtcctca
     1501 tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg ggtgtgctcg
     1561 aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat gttaatgacg
     1621 gctttatctt tctggggcac aggatcattc gcaaacgcag tcgttatggc gagatgcgag
     1681 tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg acagcactgt
     1741 tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac cgaaaactga
     1801 aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc agttatatcg
     1861 accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt acaggtatcg
     1921 cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa acgtgggttt
     1981 tatttggtaa aaccaatcac ggcaagctca gcggcgaaat actgtaccgg ttggtggggc
     2041 aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg aggacggaga
     2101 ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc agcatttaaa
     2161 tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg cagggaaata
     2221 gtccgactac
//
LOCUS       DAZSNO010000319         2218 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZSNO010000319 DAZSNO010000000
VERSION     DAZSNO010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.323"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHWN2_004277"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHWN2_004277"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR8933533.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHWN2_004278"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHWN2_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR8933534.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACHWN2_004279"
     CDS             complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACHWN2_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR8933535.1"
                     /translation="MQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          610 c          555 g          464 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac taggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DAZSNO010000320         2198 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZSNO010000320 DAZSNO010000000
VERSION     DAZSNO010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.324"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            440..1153
                     /locus_tag="ACHWN2_004280"
     CDS             440..1153
                     /locus_tag="ACHWN2_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933536.1"
                     /translation="MSQQVVIFNNEFSMSSYEFLIEVVNPARAEAGEAPVRNDQFLAR
                     VRDELDDCKTFATERITPRGGGRQMESITLTGDQLLLVGMRESKAVRRKVLDYIRRIE
                     KDKQLLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKNPISNE
                     WSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPTTVDKLRGILIAFTGTD
                     QQKQEHLLKQAQSGKLEGL"
     gene            1155..1349
                     /locus_tag="ACHWN2_004281"
     CDS             1155..1349
                     /locus_tag="ACHWN2_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933537.1"
                     /translation="MIDPRTPEGRMTLRYRGYRTEVILKELGLDPEDETRQHQSRDEL
                     IAQLVAMKLSSASKLAPAHR"
     gene            1470..1961
                     /locus_tag="ACHWN2_004282"
     CDS             1470..1961
                     /locus_tag="ACHWN2_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933538.1"
                     /translation="MLFNNQDWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRIEAERITKELASQKE
                     RTREMFMTEAEWQKELQRREQVKTERRTYGENLRSATHSAGRSKASIMADLDSGANWM
                     DSI"
     gene            2026..>2198
                     /locus_tag="ACHWN2_004283"
     CDS             2026..>2198
                     /locus_tag="ACHWN2_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933539.1"
                     /translation="MTLTKDALQFGIEPVRITDTDNLQINEGLPTNADPQVYAFQLAK
                     LVKTMLNSVLKDA"
BASE COUNT          566 a          584 c          534 g          514 t
ORIGIN      
        1 catacttcgg gtctaactgg aggtcagatt atggccttat caggtagcaa atcttatacc
       61 ctcactatag ggaatggacc cttagctatc actataggga ccaacattcc gcctctggtc
      121 ttgatgcgtc aagttgtgcg gaagctctcc cgacattaag tctttctcca taccaaggct
      181 agcaagacag cctctgtcag actcccagcc cgttagcgtt ctcgtgtctt cattttgact
      241 tgggtctatc gccgctcacc atctggacgt cggacatgct gtcttgcacc tttatcacta
      301 ctggtcatcg cagaccacat cacaattacc tcaagtcatt ctgtttgcgt cctgttgcct
      361 gctggtctcc ctttacgtgg gggcttgagt aggtcagtag ggtgcgactt cagggtgcaa
      421 acaaaaggag tacacgtaaa tgtctcaaca agtagtcatc tttaacaatg aattctctat
      481 gtcttcctat gaatttctaa tcgaggttgt caaccctgcc cgtgcagagg ctggcgaggc
      541 tccggtccgt aatgaccaat tcttagcccg tgtccgcgat gagctggacg attgcaaaac
      601 ttttgcaacc gaacggataa cacctagagg aggtggtcgc cagatggaat caatcacgct
      661 gaccggagac caattgttac tggtcggcat gcgcgagtct aaagctgtgc gtcgcaaagt
      721 ccttgactac atccgacgaa ttgagaagga caagcagtta ttggaagacc agaagaagcg
      781 agcagccatt cagtctgcta accgtcgagg cgtgacttgg ggggactact gcaaaaccta
      841 tggtctgccc gctcagaagc taatgaccgc acttcttcag catcgtggtc tctttcgtaa
      901 gaacccaatc agtaacgagt ggtctgtaaa tccaaagtat tccgactgct tccgtatcat
      961 taagccgtca gaccagaagt tctcagcagg tgggtataac ttccggttca acgcaaaggg
     1021 actggaggtc ttcggtaagc cgacgacagt cgataaactg cgaggcatcc ttattgcctt
     1081 cactggcacc gaccagcaga agcaagagca cctcctgaag caggcccaat ccggcaagct
     1141 ggagggactc taagatgatt gacccgcgca cacctgaggg ccgcatgact ctccgttacc
     1201 gtggctaccg gactgaagtc atcctgaagg agcttggtct agacccggaa gatgagaccc
     1261 gccagcacca atcacgagac gagctaatag cccaactggt cgctatgaaa ctgtcgtcgg
     1321 ctagcaagct agcgccagca catcgttagt agctcaccta cttaatacct aattttaaac
     1381 cttttgagat gaccgatgcg gggagacgcc gcaggactaa acacgcctgt agcagaccga
     1441 tagtctcaga ctcaagagga gtaattacta tgttattcaa taaccaagac tggaaactgt
     1501 cagtcaccga catcaatctc tacgagaata ctgtgagtct ggacggtcag tcctatccgt
     1561 tgtcgctcgc tatcaagacc ctgattccgg gctacttgtc cggcctgccg tctaccagcc
     1621 gcgaggcaat ggagttactg gaagctctgg ctgaagctgg cgtgaccatt ggtaacttct
     1681 tcagtaatga cctgatgact gcctaccaac gccgccagat gaataaacgt atcgaggctg
     1741 agcgtatcac taaagagctg gcgtctcaga aagagcgtac ccgcgaaatg ttcatgaccg
     1801 aggccgaatg gcagaaagag ctacagcgcc gcgaacaggt caaaactgaa cgccggacct
     1861 atggtgagaa cctccgaagc gctacccatt ctgctggtcg ttccaaggct tcaattatgg
     1921 ctgacctcga tagtggcgct aactggatgg attccatcta agaacacacg gtccctgact
     1981 taccgttagg gacttcaaat atctgactca aggagattaa ttatcatgac tctgactaaa
     2041 gacgctttac agtttggcat tgagcctgtt cgtatcaccg acaccgacaa ccttcagatt
     2101 aacgaaggtc tgccgaccaa cgcagacccg caggtctatg ccttccagtt agctaagctg
     2161 gtgaagacca tgctcaattc agtgcttaaa gacgctca
//
LOCUS       DAZSNO010000321         2192 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZSNO010000321 DAZSNO010000000
VERSION     DAZSNO010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.325"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..331)
                     /locus_tag="ACHWN2_004284"
     CDS             complement(<1..331)
                     /locus_tag="ACHWN2_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933540.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            493..549
                     /gene="ysgD"
                     /locus_tag="ACHWN2_004285"
     CDS             493..549
                     /gene="ysgD"
                     /locus_tag="ACHWN2_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR8933541.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="ACHWN2_004286"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="ACHWN2_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR8933542.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="ACHWN2_004287"
     CDS             complement(1692..2072)
                     /locus_tag="ACHWN2_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFR8933543.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..>2192)
                     /gene="yigG"
                     /locus_tag="ACHWN2_004288"
     CDS             complement(2086..>2192)
                     /gene="yigG"
                     /locus_tag="ACHWN2_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFR8933544.1"
                     /translation="EDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
BASE COUNT          568 a          522 c          513 g          589 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc gg
//
LOCUS       DAZSNO010000322         2186 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZSNO010000322 DAZSNO010000000
VERSION     DAZSNO010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.326"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(219..383)
                     /locus_tag="ACHWN2_004289"
     CDS             complement(219..383)
                     /locus_tag="ACHWN2_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933545.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1285..1569
                     /locus_tag="ACHWN2_004290"
     CDS             1285..1569
                     /locus_tag="ACHWN2_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR8933546.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1557..2042
                     /locus_tag="ACHWN2_004291"
     CDS             1557..2042
                     /locus_tag="ACHWN2_004291"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8933547.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          539 t
ORIGIN      
        1 caaaatgatg gacattattt ttgtggaacc ggaggtaaca gaccagacgg tttaaatgag
       61 ccggttactt taaacgggtg atggacagca cctcaaaagt tcagatggat tatatttgtc
      121 tgcaataccc cgggtatttt cgatttgcga aatggatgga acgacttgct caggggatag
      181 ctgatggagt tactgaagta ccgaaggacc actaaaaatc acaggccggg aatttcggcg
      241 tttccggcgt aggtgtgcca cgaatgcatc cagtggcgtg atgttggatg caatgctgta
      301 tgagagatga ggggcggcgt ccagacgcag ccttcaaacc gccttgagct gctgccgtta
      361 agagcggtgg tggtgagcgt caaggttaaa aggcgcagaa catgtgtcag gtaggtgttg
      421 cggagatgaa cgaaagtgaa ctgtcgatga agtgtcgaaa ggaacctcta ggcgatgtcg
      481 aaaatgtgct atctacgcag cgcatgacga gtccagcaga tatctggtac tggctgggcg
      541 gcatccggca tataggcagc atgaacgcaa cacaggctct cgtacggaac gtgggaacct
      601 gtcgtctcga tgctaaggga gcggcgcgga gcagaggcac tgcaagcgcc tgagtaccga
      661 tgcgagacac aggggcagat ggcttcgtag tagtgaggaa gtgcctgtaa tgggcatgga
      721 gcgaaggggg tcagtcgttg gggctggttt atcggtcaac cggatagccg ggaggagccc
      781 atgagtcagc caaagccgtt cgaggtctct aagtatgcgg tttggaaagc ataccagcgg
      841 gtcaaagcta atcgaggtgc ggcaggtgtc gatgggcagt cagtcgaggc gtttgaggtt
      901 accaatcgaa gctatacccg gtaatggcgc aggttgacct gcacttggcc aagtggattg
      961 cccgcaaaca caagcgagcg cgaggtagtc tggtgcgtgc attcgagtgg ttactgcgaa
     1021 ttcgtcacga ctgcccgacg ctgtttgcac actggtgctt agcttacgat acttgaatgg
     1081 ttcaacgaca agagccgtat gacgcgagag tgtcacgtac ggctccgtgg gagcgtaggg
     1141 gggcggtgcc cctgcgccac ccgatatccc ctttgagtgc ggttacgtcc acagaggtaa
     1201 atgccgctga cgcggcatct gtaatctgtt actttgtgta tatcatttgc tatatattgt
     1261 caatatgcta ttgttgtaaa gcaaatgata tacggaggct ttatgaaatc aggtgttcag
     1321 ctcaatctca gagcaagaga atcacagcgg atactcattg atgctgctgc agaaattctt
     1381 cataagtcgc gtacagattt cattctggaa atggcctgta aagctgctga agatgttatt
     1441 ctcgaccggc gggtgtttaa ttttaatgac aggcaatatg aagagttcat tgaaatgctt
     1501 gatgctccgg ttgcagatga tcctgccatc gaaaaattgc tggcaaggaa acctcaatgg
     1561 gatgtgtaac tgcaccagaa cctttatcct catttcatca ggtcgctgaa tttgtcagca
     1621 gtgaagctgt acttgatgac tggttaaagc agaaggagct aaaaaatcag gcaatcggag
     1681 ccacccggac ttttgttgta tgcagaaaag gtacgcaaca aatagtcggt ttttactcac
     1741 tggccactgg tagtgtcaat catacagaag ctacaggtaa ccttcggcgc aatatgccag
     1801 accccattcc tgtaattata cttgcacgcc tggctgtaga tgtgtcattt cgaggaaaag
     1861 ggcttggcgc ggatttactt catgatgcag ttcgtcgttg ttacagggtt gccgaaaata
     1921 tcggtgtgcg tgcaattatg gttcatgcgc ttacggaaaa cgcaaaacag ttttatattc
     1981 atcatggttt taagccatca aaaactcagg tgcaaacatt gtttcttaaa ctccctcaat
     2041 agggagtaat gccgatcagt taaggatcag ttgaccgatc cagtggctgt gtaagaatcc
     2101 ggaaacgctc actcgtttcc ggatttttct gtgcacagta aaagaccacc tcctgaggag
     2161 gtggcttttc agaatgcccc ctataa
//
LOCUS       DAZSNO010000323         2183 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZSNO010000323 DAZSNO010000000
VERSION     DAZSNO010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.327"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..203
                     /locus_tag="ACHWN2_004292"
     CDS             <1..203
                     /locus_tag="ACHWN2_004292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933548.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSIH"
     gene            293..694
                     /locus_tag="ACHWN2_004293"
     CDS             293..694
                     /locus_tag="ACHWN2_004293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933549.1"
                     /translation="MNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDY
                     LPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSR
                     DGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            696..1301
                     /locus_tag="ACHWN2_004294"
     CDS             696..1301
                     /locus_tag="ACHWN2_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFR8933550.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="ACHWN2_004295"
     CDS             1288..>2183
                     /locus_tag="ACHWN2_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8933551.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAZSNO010000324         2170 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZSNO010000324 DAZSNO010000000
VERSION     DAZSNO010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.328"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..955
                     /gene="espL1"
                     /locus_tag="ACHWN2_004296"
                     /pseudo
     CDS             1..955
                     /gene="espL1"
                     /locus_tag="ACHWN2_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1127..1234
                     /gene="yniD"
                     /locus_tag="ACHWN2_004297"
     CDS             1127..1234
                     /gene="yniD"
                     /locus_tag="ACHWN2_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR8933552.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1287..2045)
                     /locus_tag="ACHWN2_004298"
     CDS             complement(1287..2045)
                     /locus_tag="ACHWN2_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR8933553.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          656 a          482 c          453 g          579 t
ORIGIN      
        1 gctcagacct gggctgatag gtatgttcgc aactgtgcaa ttgctattct gcccctatta
       61 cctgtgggag ttctaggggc tattttcgcg ggtattgcat cacgaatgcc atttgcccct
      121 atacatccat cggcaatgtt gttaataatg gctacaggcc agactcaaca gcttattaca
      181 ttattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag
      241 aatagcgttg gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg
      301 aaaatattta tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat
      361 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctggacttta tatctccatg
      421 ttgtatggat tcgatgaaat aatcgatatc tttctgaata cattaaccac tccaatagca
      481 ctaagagctt ttaaacaaaa aactggtgat gagtatttta gccataaaaa tacatgatgg
      541 tgagccagga ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct
      601 ctctaaaatt aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt
      661 attaaaagga gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa
      721 tgaagacgtc gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt
      781 tagtcaacat cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt
      841 tcatatagcc attcatcata agcattataa aactgtagaa acatattatg ctgctataaa
      901 tgtcatcagc caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc
      961 tatttacgat atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac
     1021 catgtgcaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt
     1081 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
     1141 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
     1201 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
     1261 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
     1321 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc
     1381 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
     1441 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
     1501 catgcataag ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat
     1561 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
     1621 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
     1681 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
     1741 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
     1801 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
     1861 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
     1921 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1981 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     2041 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     2101 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     2161 tagcaatgta
//
LOCUS       DAZSNO010000325         2162 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZSNO010000325 DAZSNO010000000
VERSION     DAZSNO010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.329"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            311..505
                     /locus_tag="ACHWN2_004299"
     CDS             311..505
                     /locus_tag="ACHWN2_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933554.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            866..994
                     /locus_tag="ACHWN2_004300"
     CDS             866..994
                     /locus_tag="ACHWN2_004300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933555.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1304..1640)
                     /locus_tag="ACHWN2_004301"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="ACHWN2_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          688 a          444 c          450 g          580 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggctttaa
     1801 ttggaataca tatattacaa cgtattatat ataattggta ttctgggaac tatattctca
     1861 aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga
     1921 attgataaaa aaaacgccag caacacatag tgccggcgga gggaataccc catggagaaa
     1981 atgtgatgcc tagagcatca tgaatatata attaaaaata gttagcgttg tcaatacttt
     2041 cacaaaaata attttcgtag tatagaaaga tatttttatg catgacctac ctgaatttgc
     2101 tccgggtaga ggttataaat aaaaattgaa tcacgacaaa cacaatattc atagtatggc
     2161 ga
//
LOCUS       DAZSNO010000326         2123 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZSNO010000326 DAZSNO010000000
VERSION     DAZSNO010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.330"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..1068
                     /gene="ompC"
                     /locus_tag="ACHWN2_004302"
     CDS             <1..1068
                     /gene="ompC"
                     /locus_tag="ACHWN2_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR8933556.1"
                     /translation="VAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSN
                     DADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKF
                     GDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGL
                     VDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQVIYGN
                     NSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEVVAQYQ
                     FDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINLIDKSD
                     FTKASGVATDDIVAVGLVYQF"
     gene            complement(1557..2123)
                     /locus_tag="ACHWN2_004303"
                     /pseudo
     CDS             complement(1557..2123)
                     /locus_tag="ACHWN2_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          609 a          442 c          433 g          639 t
ORIGIN      
        1 gtggcaattt ctgctgtagc tgcatcagta ctgatggcga tgtctgctca ggcagcagag
       61 atttacaata aagacagtaa caagctggat ctgtacggaa aagttaatgc taagcactac
      121 ttctcttcta acgatgcaga tgatggcgac acaacttatg ttcgtctggg cttcaaaggc
      181 gaaactcaaa tcaacgatca actgactggt ttcggtcagt gggaatatga attcaaaggc
      241 aaccgtgctg aatctcaagg ttcttccaaa gataaaaccc gtcttgcctt cgctggcctg
      301 aaattcggtg actacggctc catcgattat ggccgtaact acggtgtagc atacgatatc
      361 ggtgcatgga ccgacgttct gccagaattc ggtggcgata cctggaccca aacagatgtg
      421 ttcatgactg gtcgcaccac aggtgttgca acctatcgta acaacgactt ctttggtctg
      481 gttgatggtc tgaactttgc tgctcagtat cagggtaaaa atgaccgcac tgacgtaact
      541 gaagctaacg gtgatggttt cggtttctcc actacttatg agtatgaagg attcggtgta
      601 ggtgcaacct atgctaaatc tgaccgcact aatgatcagg ttatctacgg taacaacagc
      661 ctgaatgcat ctggtcaaaa tgctgaagta tgggcagctg gtctgaaata tgatgcgaac
      721 aacatctatc tggctaccac ctattctgaa acccagaaca tgactgtttt tggtaataac
      781 catattgcca acaaagcaca aaacttcgaa gtagttgcac aatatcagtt cgacttcggt
      841 ctgcgtccgt ccgttgctta cctgcaatct aaaggaaaag acttgggtgc gtggggtgat
      901 caggacctga ttgaatatat tgatgtaggt gcaacctatt acttcaacaa aaatatgtcc
      961 acttttgttg attacaaaat caacctgatt gataagagcg atttcacgaa agcatctggc
     1021 gttgctaccg atgatatcgt tgctgtagga ttggtttacc agttctaatc tgattactca
     1081 aagatatgtt gtgggaggct ttgcctcccc aacatataag tggcgctctc aagccacttt
     1141 ccttagaagc actatcttgc ttctgactat ataaattacc ctttattttc tgggacgttt
     1201 caacgcccca tttttaataa tactcagtga acaattaacg tatcaattag atttaataac
     1261 aacgatatcc gtacctaacc tgatatctaa tgtcataagc atccctttaa ttatcccctc
     1321 aacctcttgt aaatttccca ctcttgagct ccacatagcc caaacgccgg atgcaaaaaa
     1381 aatcatacgt gcatctctga aattgtgatg gaggaagtga gtatcaatta tatgtgctta
     1441 taaacgttcc cgattggcac agaagtcacc ggattgttca ggctccaata ggttgagtat
     1501 gtcgtattta ttttccaaaa taaaaatcga tgctgttgct ccttaataaa atacttttac
     1561 tctctggacg cttttcttag ctctcttggt gttatattaa aacgattatg gaacctttca
     1621 gtaaaacgag aaggacactt gtaaccattt tccctggcaa tctcgctgat gggttttact
     1681 gtcgtttgta tagcaggaca acgcattatt taacctcacg tcgtccaata tactttgaaa
     1741 acttattccc tcgcttgcca gacggcgatg caatgtggaa acagaaatgt agagatatct
     1801 cgctaccttg tttgctgtcc attttgttct aggttcggat agcattaggt tataacaacg
     1861 acttattaat gattgtttac tatctgataa aagtaaacaa ttaacatgat gtgctcctaa
     1921 tgaaagtaga acgcccattg ctaagtgttc ctgaatttgc gttgagaagc ctcgggaaat
     1981 agatgttttt agttgctccc aacaatatat taactcgggg ttctgaggta agcagaaacc
     2041 tgtcgtgtta cgaatttgat cagttatcgc ataaaatttt tgaaactttt caattagatc
     2101 aatgggataa taaagcattt ctg
//
LOCUS       DAZSNO010000327         2111 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZSNO010000327 DAZSNO010000000
VERSION     DAZSNO010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.331"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            6..224
                     /locus_tag="ACHWN2_004304"
     CDS             6..224
                     /locus_tag="ACHWN2_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR8933557.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="ACHWN2_004305"
     CDS             226..591
                     /locus_tag="ACHWN2_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR8933558.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="ACHWN2_004306"
     CDS             588..1253
                     /locus_tag="ACHWN2_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933559.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="ACHWN2_004307"
     CDS             1253..1618
                     /locus_tag="ACHWN2_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR8933560.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          579 a          437 c          563 g          532 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt c
//
LOCUS       DAZSNO010000328         2102 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZSNO010000328 DAZSNO010000000
VERSION     DAZSNO010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.332"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(85..630)
                     /gene="ygeQ"
                     /locus_tag="ACHWN2_004308"
                     /pseudo
     CDS             complement(85..630)
                     /gene="ygeQ"
                     /locus_tag="ACHWN2_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
     gene            complement(1292..>2102)
                     /locus_tag="ACHWN2_004309"
     CDS             complement(1292..>2102)
                     /locus_tag="ACHWN2_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933561.1"
                     /translation="NIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVTA
                     PSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTQGTLRQDALNICDNVVRTINSKMVD
                     VLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVTDYGIIAHESHLKAL
                     SRLAAKQGFGGEDAIVDMLGTDIAYYNGVDRGVFMMAKRFTALSFGCFRHDGENITVV
                     LSRDGNTQSHDLEILGKVFVVAEAATTIKMGTGSAAAVLPVVKRLSFTKTS"
BASE COUNT          571 a          475 c          507 g          549 t
ORIGIN      
        1 gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca gaacctcgct taggcctgtg
       61 tccatattac gtgggtagga tcagaattcc atccagtcgt tttgggttgc taatgatgtc
      121 ctgcatccgc ttaatctggt cgggcgtaga catataaccc cacagggtga gcagaacctt
      181 ttttgcggct acactatttc cttggtctga tactaatgcc atatagcccc ataaactatc
      241 cctcagccag acagcatcat agtgggtttc tgccgctgtt ttgtcatctt cgaaagtaga
      301 agcaagaata aacgttcccc agggtttctc cgtcaacgtt aactttcgtg tgttttctgt
      361 taatgttagc aatccttgag tttcgtcttt ggtatagtga ctctttattt cattttttaa
      421 tttggctgag tgaatcagaa gacgataatt aggatactta tctggattta acgataaata
      481 acttttatct gttgctgccc ccctggaatt aagaatgtta aatacagatt cgcttgtcac
      541 ttctgcctct gtcgcctggc atgtgccgca cgaaaatata ccagcgagca gaagagttaa
      601 tgcagatttc cctttcataa aataatccat caatcgtttg ttaaaagcgt agataaccac
      661 ttattcaaca acttgaaaat aaggtatttt acctgagttg ttacaaaagg attgcattgc
      721 gtaaacgctt tttatttaca aaaaaatggg gaagtgttac agcgattgcg aaaagcctga
      781 agtgattgaa ttaagataat atagaaaatc agatacagca aaaatggcag attgatcagg
      841 tgaagataaa ttggagcggg cgaagggagc cgaggacaac tagctaactt cttgtttctt
      901 aaagactgat gaatccagtg gttacagtga tggttacaca tcgagaggct gacggtcaag
      961 acttgaagca cgtaatattc ccataaaagg aatagaacac agtcactgac tacccggccc
     1021 tttggtctga ctgagggtcg ggctatgggg taaatcgtca ggcttattgt attagatggt
     1081 ctctcacatt tttcagtcaa aaactcatcg tactttaggc cagaccatca gcacgactac
     1141 cagagctagc tttagcagga agaccacgag atagactttg tgtaggtcca ttaggtcctc
     1201 ctctttccgt acccatgaac tagattgcta gtacaaaata aaggccagac atccagttac
     1261 ggaagtctga ccaataagtc gttatcgtta gttagcttgt cttagtaaag ctcaggcgtt
     1321 taactacagg caggacagca gcagcgctac cagtccccat cttgattgtc gttgcagcct
     1381 cagcgaccac gaagacctta ccgagaatct caaggtcgtg agactgggtg ttgccatctc
     1441 gggacaggac cacagtaatg ttctcgccat cgtgacggaa gcaaccgaag ctcagagccg
     1501 tgaagcgctt agccatcatg aatacaccac ggtctacacc gttgtagtag gcgatgtctg
     1561 tccccagcat atcgacgata gcgtcttcac caccgaagcc ctgcttagca gcaagacggg
     1621 agagagcctt gaggtgtgac tcatgggcga tgataccgta atcagtaacc tcagaaccga
     1681 aagaagactc ggtgttctcg tacagggcat ccatgatagc gtctgctttg gccgtcaggc
     1741 tcccggtcag gacaggaagc tcgacggtct tcagcagctt agaggactcc agaacgtcaa
     1801 ccattttaga gttaatggta cggaccacgt tatcgcagat gttcagagcg tcctgccgga
     1861 gcgtcccctg agtgaaacca gtagaggact tctggtccca taacttggtc tggtgggaca
     1921 cgaggatagc ttgctcaatc ggctcgacgg tgatttcagt accagacgga gcggtcacga
     1981 cttcaggtcg cttaccacca cggacaggaa cgaccacaga gcggtcagcc agagtgtgag
     2041 caattattgg cgtcggtaag ctgttacggg tcggcaggac ttctacaggg aacggaatgt
     2101 tt
//
LOCUS       DAZSNO010000329         2092 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZSNO010000329 DAZSNO010000000
VERSION     DAZSNO010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.333"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="ACHWN2_004310"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="ACHWN2_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR8933562.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHWN2_004311"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHWN2_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR8933563.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DAZSNO010000330         2067 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZSNO010000330 DAZSNO010000000
VERSION     DAZSNO010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.334"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..704
                     /locus_tag="ACHWN2_004312"
     CDS             <1..704
                     /locus_tag="ACHWN2_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR8933564.1"
                     /translation="FPTYAADDVKVTQNTSVFKLKLSATRLIYEQGSDGAAITVTNPQ
                     DWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKMWVVQTAPALQDREKLHWLCA
                     TGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLITRPSGIEGKMVNASEKLSWK
                     KQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFSQHEFVMPKTSGQDIKWSVIT
                     DEGGESRLFISHLQS"
     gene            778..2067
                     /locus_tag="ACHWN2_004313"
                     /pseudo
     CDS             778..2067
                     /locus_tag="ACHWN2_004313"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          597 a          377 c          506 g          587 t
ORIGIN      
        1 catttcccac ctatgctgcg gatgatgtca aagtgactca aaacacttca gtttttaaac
       61 tgaagcttag tgcaacgcga ttaatctatg aacaagggtc ggatggcgca gcgataactg
      121 tgacaaatcc gcaggattgg ccgatgcttg tgcagagtaa agtctttgaa gaagataaag
      181 taactgcagc gccctttatt gtcactccgc cactgttcag gcttaatcca ggccagcaaa
      241 ataaaatgtg ggttgtgcag actgcgccag ctttacaaga cagggaaaaa ttacactggc
      301 tatgcgccac cggcattacg cctaaaaaga gtgatgtttg ggccgagaaa gataaagaaa
      361 aaattgctga aaatgtgaac ctgaacatcc aggttaacgt acatacctgc ataaaactta
      421 ttaccaggcc atctggtatt gaaggcaaga tggttaatgc gagtgagaag ttatcgtgga
      481 aaaagcaggg tgataaaatt gtcgcggtca atcagtcgcc gttctacatc aacctttctt
      541 ctttacaggt tggcggtagc aagattgaga aactagatta tattgcgccg ttttcgcaac
      601 atgagtttgt tatgccaaaa actagcggcc aggatattaa atggtctgtg attactgatg
      661 aaggtggtga gagtcgtttg tttatatctc atttgcaatc ataatgtatt acatttttat
      721 tgaatatttt tatgccatgc atattccatg ttttatcgac tgtcattaat tttgataatg
      781 gccttacttg cgggtcagtt aagtgcccag gagtggtcat ttgattcttc acagttggaa
      841 ggaaacgtta gtgcggacac tgtggcaatg tttaatcagg gagagcaact ccccggaaat
      901 tatcgtgtgg aaatttatct taacggtgaa aaagttgatg ttggggagtt tccctttcat
      961 cgtccggaat cccctgaaga aaaagagctt gttccatgtt taaccgttga tgaccttatc
     1021 cactatggca ttaaaattga taaaagcagc agtgatactg acaataaaaa gaatcagtgt
     1081 tttaaatgga actctattga aggtttgaaa gtaaattatg attttgatag ccagcgcgta
     1141 cagataaccg ttccacaact gtatctgcaa gataaaaaaa gtagtttagc accagtgtcg
     1201 ttatggaatg agggtgtagc tgcattccgc atggtgtatc agacaaatat tgatatttcc
     1261 aaacaaaatg ataatcaatc taccacgcgt aattctcgtt atggcaggtt cactccaggg
     1321 tttaatcttg gtgcctggag gtttcgtagt tcagtgacat ggagcaagga acttggccag
     1381 tcagaaagat ggcaaagggg ttacatgtgg tttgagcgag gcataaatgc gattaaaagc
     1441 cgattaacgc ttggagaatc ttatacgtcg tctgaggtgt ttgacagcat accttttcgc
     1501 ggcgggatgt tagcgaccga cgatgcgatg actccgccgg aggacagcta ctacacacct
     1561 gtggtgcatg gaatagcaca aagtgaggcg caggttatta ttaaacaaaa tgggcaaatt
     1621 atttttaccc gttctgtgcc gccagggcct tttgcgctgg ataatttacc aacgctggcc
     1681 gttggcgggg agcttgatgt tacagtgaga gagagtaatg gcgaggagca gtatttttcc
     1741 gtgccatttc aaacgccggc catcgctctg catgaaggat atttcaaata tagtgtgatg
     1801 ggggggaata taaagaagaa ggtataaaaa gctcccctgt ctccgtgggc gaaatcacgg
     1861 cgatgtacgg gcttccttat ggcgtcacgg tgtatggagg gattcaaaac gccactcatt
     1921 ttaatgcaat atctacgggt attggcattt ctctggggct gttagggagt ttatcgacag
     1981 atattacgcg ttcgattgcg aatttatatt atgggaataa atatcgtatt cgttatagta
     2041 aatccattag tgattttggt actcaat
//
LOCUS       DAZSNO010000331         2066 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZSNO010000331 DAZSNO010000000
VERSION     DAZSNO010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.335"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..374)
                     /locus_tag="ACHWN2_004314"
     CDS             complement(<1..374)
                     /locus_tag="ACHWN2_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR8933565.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
     gene            complement(544..825)
                     /locus_tag="ACHWN2_004315"
                     /pseudo
     CDS             complement(544..825)
                     /locus_tag="ACHWN2_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(972..1832)
                     /locus_tag="ACHWN2_004316"
     CDS             complement(972..1832)
                     /locus_tag="ACHWN2_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR8933566.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          550 a          539 c          475 g          502 t
ORIGIN      
        1 ttgttgagga ttttacccag aaaccggtat gcagctttgc tgttacgacg ggaggagaga
       61 taaaaatcga cagtgcggcc ccggctgtcg acggcccggt acagatacgc ccagcggcca
      121 ttgaccttca cgtaggtttc atccatgtgc cacgggcaaa gatcggaagg gttacgccag
      181 taccagcgca gccgtttttc catttcaggc gcataacgct gaacccagcg gtaaatcgtg
      241 gagtgatcga cattcactcc gcgttcagcc agcatctcct gcagctcacg gtaactgatg
      301 ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa atgccggcct
      361 ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta tcaccaccga
      421 ctatttgcaa cagtgccata tcaagcgact tctcctatcc cctgggaaca catcaatctc
      481 accggagaat atcgctggcc aaagccttag cgtaggattc cgccccttcc cgcaaacgac
      541 cccaaacagg aaacgcagct gaaacgggaa gctcaacacc cactgacgca tgggttgttc
      601 aggcagtact tcatcaacca gcaaggcggc actttcggcc atccgccgcg ccccacagct
      661 cgggcagaaa ccgcgacgct tacagctgaa agcgaccagg tgctcggcgt ggcaagactc
      721 gcagcgaacc cgtagaaagc catgctccag ccgcccgcat tggagaaatt cttcaaattc
      781 ccgttgcaca tagcccggca attcctttcc ctgctctgcc ataaggggtc tgacgctcag
      841 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      901 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      961 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1021 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1081 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     1141 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1201 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1261 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     1321 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1381 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggcagca
     1441 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1501 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1561 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcaccaca tagcagaact
     1621 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1681 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1741 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1801 ataagggcga cacgaaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1861 atttaccagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1921 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     1981 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
     2041 ttttataaac cgtggagcgg gcaata
//
LOCUS       DAZSNO010000332         2030 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZSNO010000332 DAZSNO010000000
VERSION     DAZSNO010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.336"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(3..891)
                     /locus_tag="ACHWN2_004317"
                     /pseudo
     CDS             complement(3..891)
                     /locus_tag="ACHWN2_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          547 a          474 c          471 g          538 t
ORIGIN      
        1 tatcacttaa ataagtgata ctggttgtct ggagattcag ggggccagtc tagcagctgc
       61 tctacggtca tatttttaat tgcgctccgg tttacaagag cccacccctg tttttccaga
      121 taaaactgga aagtatccag ggtacagacc agtgcaccgt caggaacagt ttcggtgaat
      181 ctgatattgc cgtgttcgtc gaagtgcaca accagagtgc gaccgttacc cggaatcatc
      241 ttgtcagcgg acggggtatt attctggtgc agttctgcct ccatgcggtc gaacatcagc
      301 gatgtaggcc tctttgaatg cagcggcttt tttcccagtg aagcccatca ccaggaaaac
      361 gaagccgttt ttggtgattt ggtacattgg gcgtttttcg cctttggtgt cggtgtaggt
      421 gacgtcctca aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg
      481 taacacattg tcgtgtcgtt tgtggaagaa ctctgcaacc gcaacagacg tagtgacagc
      541 gcgaccgttt tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc
      601 atcctcgagt gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt
      661 gagacgtaca ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc
      721 tgcacatccc accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc
      781 gcggctatgc gctcgagtaa cgttcgggtt tccacgcccg gcccccgttt tatgaggtgc
      841 agatgcacta taattccacc cgttctggtt ttcaatagct acattcaaca ttttctctta
      901 cctttcatca ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa
      961 ccagttaaca attaacgccg gatacaggga aacccataac cacccgatac gtgggaacta
     1021 atcgcttttt aactgttccg cgtgcgctca gccgcattca ccgcatcaca aaattcactt
     1081 taaaaagggc ggacatcagc cagcaatgaa actgatgccg ccaaaggtac caatcaacat
     1141 ggagtgttgt ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc
     1201 aacggggaag gaataactcc gccatactta ccgccgcgtc atttcgcgtt gtgtgcctgc
     1261 ttttaactac gtcaggcgag gtggttcctg ttattcccca acaacaagaa atttgtataa
     1321 tctgaatacc ccaacaataa gaagagtgag caagtgatgt attagcgatg tcatctctca
     1381 aagagactgc atcaatgtta aaattattca atgaagcaaa aactcaggct gaggttgatc
     1441 gcgctgtgtc tgaaataaac tctaagttgt caagcattca gcttgaatat ttgtcatctc
     1501 attgatgagc tgacaatctc taaaagcaat gaggctgcgc tcaaagcaaa actcacggag
     1561 atagaaaagt ttaagttcca aagtgaagac tgcgaacctc gctaattgat tactggaacg
     1621 attgtgtatg ccagacagtg caatactagc tctggcaaga ttaccatgta tttttgtcca
     1681 gaatgttttg cgaagagagc aatatctata ttgcaacctt tccctatcga ggaaggcgat
     1741 aaatatacga aaactcaatg cccctcctgt aaaaatgagt atctcatgga ctacaatttc
     1801 aataatgatt tttgagtcac ggtattcaga tttttaaaga acatgccgga tactcacccg
     1861 tgtccggcgc acgcactcca cctcacccgt ggagaactcc ttaattacca accttggctt
     1921 cgttggttag ccattaacgc tggtatgtaa tcattctggc aatgcttaat ttgaagtggc
     1981 acactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa
//
LOCUS       DAZSNO010000333         2029 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZSNO010000333 DAZSNO010000000
VERSION     DAZSNO010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.337"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1824)
                     /locus_tag="ACHWN2_004318"
     CDS             complement(<1..1824)
                     /locus_tag="ACHWN2_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR8933567.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="ACHWN2_004319"
     CDS             complement(1932..>2029)
                     /locus_tag="ACHWN2_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8933568.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAZSNO010000334         2006 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZSNO010000334 DAZSNO010000000
VERSION     DAZSNO010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.338"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(17..286)
                     /locus_tag="ACHWN2_004320"
     CDS             complement(17..286)
                     /locus_tag="ACHWN2_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR8933569.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            343..1011
                     /locus_tag="ACHWN2_004321"
     CDS             343..1011
                     /locus_tag="ACHWN2_004321"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR8933570.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1327..>2006
                     /locus_tag="ACHWN2_004322"
     CDS             1327..>2006
                     /locus_tag="ACHWN2_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8933571.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          607 a          432 c          372 g          595 t
ORIGIN      
        1 ggtttttctt tttcgcttac attgtgtctg gttcttccgg ccactcaata tcaggtgcag
       61 ttgatgtatc aacacggttc agcaacaccc gatacttctt ccaggcttcc agcaacgagt
      121 tttcttcctc cgttgcgatc tccagatcta cagcatcctg aagtggcgca atatgctcac
      181 tgaatccctg gatgtagaac tgtgtggtga cggtcttcca gccattcggc tcctgctgta
      241 tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg cagcatttaa ccccttgtaa
      301 ttcatcgcca taattgattt aattcacaaa taaaactata atatggtgaa attaatgaaa
      361 aaaaacacag atgatggggc taaaatttac acaccactta ccctaaagct ttatgactgg
      421 tgggttttgg gagtatcaaa tcggcttgca tggggatgtc ctacaaagga acaccttctt
      481 ccacactttc tggaacattt aggtaacaac catctggata ttggtgttgg aactgggttt
      541 taccttactc acgtacctga gagtagtctg atatctttaa tggatttgaa cgaagctagc
      601 ctgaacgcgg catctacaag ggctggggaa tcaaaaatta aacataaaat tagccatgat
      661 gtttttgaac cttatcctgc ggcgttacat ggtcaatttg attccatttc catgttttac
      721 cttcttcact gcctgcctgg aaatatatct acaaaaagct gtgtaatacg caatgcggcg
      781 caggccttaa ctgatgatgg aactctatac ggagccacaa ttcttggcga tggagttgtg
      841 cacaatagct tcggtcaaaa actgatgcgc atttacaatc agaaaggcat cttttcaaac
      901 acaaaagatt ccgaagaagg cttaacacat atactctcag agcatttcga gaatgttaaa
      961 accaaggttc aaggtactgt agtaatgttt tccgcttcag ggaaaaaata gcatccaacc
     1021 gcagcacgtt cttgcttaag acgtgctacg gtttccttta cgatttgggg ttgcgacttt
     1081 acctctattg ataatgcatt ccggcagaac gttcaaatat gaacgtacga tatttaacta
     1141 accgaaaaac aaaatatagc acaggcgaga catttaccat ctaagaaaat agtatcgttt
     1201 ttttacagtt aaatcaacat cacttcctta aaatgaaaac aataaatttc aatcctgaaa
     1261 cattttattt cagcttatta ttccccagta tcacatttta tcaggatatc cttctgcgag
     1321 gttattatgc ttcctgtaaa taatcccccc ctatccactg gaaacgtctc tttttacaga
     1381 actacatcaa tcgacaatgt tcacaataat tatctctccg aatgggttga atggactaaa
     1441 aacagcattt ccggagaaaa cagggaaact gcttttaccc ggctccaatt atgtctggag
     1501 aacagtgaaa catcgttgga cttatcttgt ttaggtctca gatctctacc acgattgcct
     1561 gacaatcttg atgaaattaa tgtaagcaat aaccaactat caatgctccc cgagctacca
     1621 agggcattga aagagctgaa tgcaagcagt aatcaattat ctgcacttcc tgaattacca
     1681 gtgtcgctgg aatatataaa tgtgagtgat aaccatttgt tcgcacttcc tgaattacct
     1741 gcgtcactag aatatattaa tgtaagtgac aatcacctgt ctgtacttcc gaggttacca
     1801 atgtcattgg aattacttga tgcagccaga aatgctttgg aagtaatacc agattttcca
     1861 gaaagagatg atcatattat aagaatattc tggcttaatc agaaccggat cacggcaatt
     1921 ccggaaagca tacttggcct cagttctgat agcgttgtca atcttagaga aaatcaacta
     1981 tctcccagaa taatgcaaac tttgtt
//
LOCUS       DAZSNO010000335         1987 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZSNO010000335 DAZSNO010000000
VERSION     DAZSNO010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.339"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>1987
                     /locus_tag="ACHWN2_004323"
     CDS             <1..>1987
                     /locus_tag="ACHWN2_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR8933572.1"
                     /translation="DYKISLSPDTTYRELNKAAQSSITAPDRIGEGKTWVYQRDTMVE
                     RGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDYELAADVYNINVTSPSGQQ
                     ETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEAILHAFNATYQQIRENMSE
                     FARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFIALV
                     KDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKISKDIESGIPGYWQGQVLI
                     PFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKNLHALKEKSLNGMPKTTPK
                     SDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSITPPLKNYNERLHNTNLFN
                     PKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGDLLT
                     LASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALPFSR
                     PLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWSA
                     FEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGTWPNKLQPGIYKNMGSN
                     SVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLL"
BASE COUNT          688 a          396 c          373 g          530 t
ORIGIN      
        1 cgattataaa atttcccttt ctccagatac aacttaccga gagctgaata aagctgcaca
       61 gagtagcatt acagcgccag acaggatagg agaagggaaa acatgggttt atcaacgaga
      121 tacaatggtt gaaagaggtg ataacagtgg tgttcatcag tatggtccag ctgaacattt
      181 cactcacatt atatctgaca aaccgtcccc aaaagataag tatgttgcat atgctattaa
      241 cattcctgac tatgagctag cagccgatgt atataatatt aacgtgacgt caccttccgg
      301 acagcaagaa acatttaaaa tactaatcaa tccagaacat ctacggcaaa cacttgagcg
      361 taaatctctt actgctgttc agaaatcaca atgtgaaatc atcaccccca aaaaacctgg
      421 cgaagcgatt cttcatgctt ttaatgccac ctaccagcaa atcagggaaa atatgtctga
      481 gtttgcacgt tcccattatg ggtatataca aatccctcca gtgacaactt tccgcgccga
      541 cggaccagaa actcccgaag aagaaaaagg ttactggttt cacgcttatc aacccgaaga
      601 tctttgtacc atccataatc caatggggga tttgcaggat tttatcgcat tggttaaaga
      661 tgctaaaaaa tttggtatcg atatcattcc tgattatacc tttaacttta tgggaatcgg
      721 gggaagtggt aaaaatgacc tggattatcc ctctgctgat atacgagcga agatcagtaa
      781 agatatagaa agtggtatcc ctggctattg gcaaggtcag gttttgattc cattcattaa
      841 agatccagta acaaaagaac gtaaacaaat ccatccagaa gatatacatc tcactgcaaa
      901 agacttcgaa gcaagtaaag ataacatctc taaggatgaa tggaaaaacc tccatgcatt
      961 aaaagaaaag agtttaaatg gaatgcctaa aacaaccccc aaaagtgacc aggttattat
     1021 gttgcaaaat caatacgttc gtgaaatgcg aaaatatggc gtacgaggtt tacgttatga
     1081 tgcagcaaaa cactcaaaac atgaacaaat agaaagatca ataaccccac cgcttaaaaa
     1141 ttataatgag cgattacaca atactaactt atttaaccca aaatatcatg aaaaaaccgt
     1201 tatgaattac atggaatatc tggtaacttg tcagttgaat gaagaacaaa tgtcatcgct
     1261 gctttatgaa agagatgatt taagcgccat tgatttttca ttgctcatga agacgataaa
     1321 agccttttca ttcgggggag atctcctaac ccttgcatca aaaccgggtt ccacaatctc
     1381 aagcatccca tcagaaagac ggatattgat taacattaac cacgattttc ctaacaatgg
     1441 caatcttttc aatgactttc tatttaacca tcaacaagat gaacaattag caatggcata
     1501 tatggccgct ctcccgttca gcaggccttt agtttactgg gatggccagg tattaaaatc
     1561 aacgactgaa attaaaaatt atgatgggcc cacgcgtgtc ggtggtgagg cgtggcttaa
     1621 taaaggttgc tctacctatc agcagctcta caatgaattc cacgcattat atatagataa
     1681 agcaggaata tggagcgcat ttgagggtgt atttgcaact aaaaacgttc tggcctttag
     1741 tcgtggggat tctgtgaaca ttaatcactc tcctcatgat ggactagtta taataaataa
     1801 aggaaacgaa gaagttgaag gtacctggcc taacaaattg caacctggaa tatataaaaa
     1861 catggggagt aatagcgtta acattattat taataatacc cgaaaaatta tccccccttg
     1921 taaagtattt acgcttagag gcggatctct aaatatcaat attcctaggc gtagcgctct
     1981 tctttta
//
LOCUS       DAZSNO010000336         1969 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZSNO010000336 DAZSNO010000000
VERSION     DAZSNO010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.340"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            240..509
                     /gene="yacA"
                     /locus_tag="ACHWN2_004324"
                     /pseudo
     CDS             240..509
                     /gene="yacA"
                     /locus_tag="ACHWN2_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
     gene            509..678
                     /gene="yacB"
                     /locus_tag="ACHWN2_004325"
                     /pseudo
     CDS             509..678
                     /gene="yacB"
                     /locus_tag="ACHWN2_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="ACHWN2_004326"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="ACHWN2_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFR8933573.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1708..>1969
                     /locus_tag="ACHWN2_004327"
     CDS             1708..>1969
                     /locus_tag="ACHWN2_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8933574.1"
                     /translation="MTEALRLHFEEHLPRVVAGCRLGVPKSTVCGMFVRFRNAGLSWP
                     LPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRR"
BASE COUNT          560 a          432 c          462 g          515 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgtctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggc
//
LOCUS       DAZSNO010000337         1955 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZSNO010000337 DAZSNO010000000
VERSION     DAZSNO010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.342"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..676
                     /gene="phoN2"
                     /locus_tag="ACHWN2_004328"
     CDS             <1..676
                     /gene="phoN2"
                     /locus_tag="ACHWN2_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR8933575.1"
                     /translation="ALKAEGFLTQQTSPDSLSILPPPPAENSVVFQADKAHYEFGRSL
                     RDANRVRLASEDAYYENFGLAFSDAYGMDISRENTPILYQLLTQVLQDSHDYAVRNAK
                     EYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHASFGWAVALILAEINPQRKAEIL
                     RRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLHNTPEFTKSLSEAKKEFEELNTP
                     TNELTP"
     gene            749..871
                     /locus_tag="ACHWN2_004329"
     CDS             749..871
                     /locus_tag="ACHWN2_004329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933576.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(1029..1955)
                     /gene="ospC4"
                     /locus_tag="ACHWN2_004330"
                     /pseudo
     CDS             complement(1029..1955)
                     /gene="ospC4"
                     /locus_tag="ACHWN2_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
BASE COUNT          595 a          392 c          358 g          610 t
ORIGIN      
        1 tgctctgaag gcagaaggtt ttctcactca acaaacttca ccagacagtt tgtcaatact
       61 tccgccgcct ccggcagaga attcagtagt atttcaggct gacaaagctc attatgaatt
      121 cggccgctcg ctccgggatg ctaatcgtgt acgtctcgct agcgaagatg catactacga
      181 gaattttggt cttgcatttt cagatgctta tggcatggat atttcaaggg aaaatacccc
      241 aatcttatat cagttgttaa cacaagtact acaggatagc catgattacg ccgtgcgtaa
      301 cgccaaagaa tattataaaa gagttcgtcc attcgttatt tataaagacg caacctgtac
      361 acctgataaa gatgagaaaa tggctatcac tggctcttat ccctctggtc atgcatcctt
      421 tggttgggca gtagcactga tacttgcgga gattaatcct caacgtaaag cggaaatact
      481 tcgacgtgga tatgagtttg gagaaagtcg ggtcatctgc ggtgcgcatt ggcaaagcga
      541 tgtagaggct gggcgtttaa tgggagcatc ggttgttgca gtacttcata atacacctga
      601 atttaccaaa agccttagcg aagccaaaaa agagtttgaa gaattaaata ctcctaccaa
      661 tgaactgacc ccataaagct ggacagcctg tatcaggcta tggagggccc atagacaaat
      721 ctaccctata tgagcatagg aggagtctat gggcacacca cgttttaccc ctgaatttaa
      781 gggattactg gaaaggctgg gacatatccc tccggcagaa gcagaaaaag cttattatgc
      841 tgccatcgga aacgatgatc tggcaacctg agttcacaga taaaacattc tctaggaaac
      901 tcggggcggt tccgttcacc acatgcaatg tggtgttgca ggggaacggt ctgcaccatc
      961 ccctatgtcg atcaatataa cagaaatgac aacttcagat tcagggcaca acctaaatat
     1021 attttaggtc acctctcaaa tcgtttgcct gataccgctc cattttttaa taaaaaaatc
     1081 aatcattttt gaatctttgc ttttcattgc attgtcaagc atagtgtccc cactatttgc
     1141 tttttgaaat tttttgttta catcaaccaa tccattattg ataaaatatt ctagcacttt
     1201 atagttggca ccatctttac taagtaagta ttcaacatca tacagattac aatatctatt
     1261 gtttttcatt ttctccatct ctgcgacatc ttgttttgta aaattaaatt tagatagtaa
     1321 ataaagtgct atatcagctt ttttattgcc taaagaataa gcaagggcct gatgagccat
     1381 ttttttatca gtcactttat tgttaatctc ttcataatta gatgctttaa tcgcatcctc
     1441 caaagatatg tctctgagtt ttattttttt gaagttatct gtacttagca ttcttggtat
     1501 atgatactca agctgaaaca caaagtttat aattctatcc agactaacag gatcaatatt
     1561 tttgttataa caaaactctc tgaatccttg atctttactc tttcttataa aatcaataag
     1621 atgcaaccca attccaagcc tcatatcttt agtgttaaat attggaacat cagttttccc
     1681 ctgattacca tgcacatacc tgctaacttc atccacaacc tctttaaagt tatctaagaa
     1741 aaatgattgg tgctcatggt aaaaaacagg tggaatagca ttatcaaagt gatcggttaa
     1801 tgtcatatat ccatatggag agtcatgatc aatgatatac gcattggcgc caaaatctac
     1861 agtatgatgt gttttattta gtgggagttt ttcttgatga ttggaaattt cgaccccaaa
     1921 aaaaacaaaa tcgtggttag agatttcttt aatat
//
LOCUS       DAZSNO010000338         1946 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZSNO010000338 DAZSNO010000000
VERSION     DAZSNO010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.343"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..284)
                     /locus_tag="ACHWN2_004331"
     CDS             complement(<1..284)
                     /locus_tag="ACHWN2_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFR8933577.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLV"
     gene            complement(317..814)
                     /locus_tag="ACHWN2_004332"
     CDS             complement(317..814)
                     /locus_tag="ACHWN2_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR8933578.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRD"
     gene            complement(880..1509)
                     /locus_tag="ACHWN2_004333"
     CDS             complement(880..1509)
                     /locus_tag="ACHWN2_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933579.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1513..1728)
                     /locus_tag="ACHWN2_004334"
     CDS             complement(1513..1728)
                     /locus_tag="ACHWN2_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR8933580.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          505 a          518 c          418 g          505 t
ORIGIN      
        1 ttgacgagca cccgaccacc attatcaagc ttgcgccgaa gagcatcatt ttcagctttc
       61 gcatcagcta actccttcgt gtatttagca tcgagtgcat cagcatcacg ctggcgctgc
      121 tgcatgtcag taatggttgc gttcgccagc ttcagttctc cggcgttttt gtcgcgctgc
      181 tctttgtagg taatggcgtt atcacggtaa tgattaacag cccatgacag gcagacgatg
      241 atgcagataa ccagagcgga gataatcgcg gtgactctgc tcattgatct atcccccaac
      301 aggctaatgc gctttcctag tcacgacgaa taacctgtcc atagcagtta tttgaacgta
      361 tgcggcaatc gcgcccaaca tcttttatcc accagcgaat cgcctcgcat gcgcccttac
      421 gatcaccggc attcagccgc ttataaaacg tcgacgggaa acacttaccg gggccaatgt
      481 tataggggca aaatgacgct atacccgctt tctggggttc ggtcagaggt actttaatat
      541 tgcgctccac ccatgccagc gccttatcac gttcaatagc gttaacctgg tcacattttt
      601 ccttcgacag cttcattccc ggtatgacag gcttaccatc caccattgtg gcaccacgac
      661 agatggtcca tatgccagaa ccatcacggt atgccgttgt gtgattaccc tctttttcgt
      721 tcagaaactg gtcaagtatc tggggagcag acgcgcctgc ggcaatcagc gccagaacag
      781 cagccgacag gccgtatttg atttttgcgc tcatggatat ttatcaggat ttatcggcaa
      841 caaataacga gccaacttat atacgccttt taagataagt cagccccggc tggatccagt
      901 catcaggctc tttcttaaag ggggagtatt gaaatcacga agaagagcct cccgcaccgc
      961 agcatccatg tctgcaccac tggcaagtgc ttcaatctct gccgccacct gcaaataccc
     1021 catgcaacgg ccaatgcgtt tcataactct ggtgttttcg tctttcaggc atctgatagc
     1081 gagattaata attttcaggc agtgatgatg cgatgcccgg acaggttttc cgttctcata
     1141 aacgacgaac tcaccctctt cacaggaaac cctgaaaggc cgatggggtt cctggtcttt
     1201 atccggtgca gacgcaataa aatcactttt atgctcttcc tctccggcac tgttaaccgt
     1261 aatgacgtac tcaggccgca ggtaagccgc ctctttttct gacaatgcgg ccggtaaaaa
     1321 cttttcctgc aaaaacttca taaaagtctc gtgagcgact ttgtggcagt aatcgtaaat
     1381 cgccgcataa tgctcatcgc ggcgtttgtt ttcatcttca gaactcacca acgccgacaa
     1441 ttttttatta agctcagtga tttcattttc caggtgattg aatcgctgat tcatttcttc
     1501 atgcttcatt atttactctc cccgtgcagc cttacgcttg tcttctctta ttttgaaata
     1561 cagattcgtc agataagtca gaagccccag aagcagactt cccagcacac caatcgcagc
     1621 ccactgtgat ggcctgacct gatccaacca ctgcaaaaac cagtagccgg cactgccggc
     1681 ggaggtgccg taggcaatgc ccgttgaaat tttgtccatg gatttcatag cctcacctcc
     1741 gcaaataacg gatggcgtag ttttacactg agaaatgaaa gggatttgaa aagaaaaaaa
     1801 cgcaaaagcg ggcgaaacga tatatacagt aaggaaagca ctctatccaa caaaccaccc
     1861 acagttaatc ggaataaaag cagagtgctt atgaatgatc gcacgcccga aggttagtat
     1921 ttctgcacag caattttgca aaaaaa
//
LOCUS       DAZSNO010000339         1935 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZSNO010000339 DAZSNO010000000
VERSION     DAZSNO010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.344"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACHWN2_004335"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACHWN2_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR8933581.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="ACHWN2_004336"
     CDS             complement(590..1255)
                     /locus_tag="ACHWN2_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR8933582.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(1424..1780)
                     /locus_tag="ACHWN2_004337"
     CDS             complement(1424..1780)
                     /locus_tag="ACHWN2_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933583.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            complement(1838..>1935)
                     /locus_tag="ACHWN2_004338"
     CDS             complement(1838..>1935)
                     /locus_tag="ACHWN2_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR8933584.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
BASE COUNT          466 a          502 c          430 g          537 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgtgtga ctgctccccg ccctttcggg cggtctcctg atgatttgag ggtgcagaaa
     1381 tccctccggt taaggattaa atttttaaca gtgctaaatt taattattca gttctggatt
     1441 ttgtcgccct gcgtatccgc gctttcgcgt tacgctcaat ctgtatcagc ttttctatat
     1501 ttctccgcct ttcctgttcc tcctggcgca atagccttac atcatctgcc agtctggttt
     1561 ctctttttgc cacagagagc atccagtcaa acggctccac aaccgcaccg cagattttac
     1621 agcggacctg acgctctttt tcgtcaaccc gtacagaggc gtgatggcaa tatggtcttt
     1681 ccgatggctt ataaagaaaa ttaacctgat tacgagggtc atcctctttt atcggaaata
     1741 aaacgatatt gcttaactca tcttctggtt ttatttccat gctcctctcc tttgatgcga
     1801 atgccagcgg taattgaagc ctgatagcta atttcattca cagtatcgcc tcctgaaaat
     1861 taccctgata gaaagccagt acacgctgca tagcttcgct cttccggcac tcgcgacaga
     1921 ttatattcag gcgcc
//
LOCUS       DAZSNO010000340         1919 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZSNO010000340 DAZSNO010000000
VERSION     DAZSNO010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.345"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(59..1708)
                     /locus_tag="ACHWN2_004339"
     CDS             complement(59..1708)
                     /locus_tag="ACHWN2_004339"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR8933585.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          510 c          491 g          466 t
ORIGIN      
        1 cctcctgaag aggataattt tacagccagc aggaagttag cggctggctg taaaaatatt
       61 atggttcgcc gtacaaacca attaaacggc gtaccacacc gttagtccag ccaaacccat
      121 cctgcaacgg atactcgcca ccgccgcctt cgcggggaac accatcggca atatggtatt
      181 tctcgatcat tttgtgctgt tccagataga actgattcac cgttttcagc cagctgcgcg
      241 cgatttcatc acccagaagg tcatcgccat acattttaaa tccctgaatt gccatccatt
      301 gtaacggtgc ccagccgttg ggtttatccc actgttcacc ggtttcgtac tcgcttgcca
      361 aaatcccgcc aggtgtcagt aaccggctgc gtacggcgtt tgccagacga tcggcctgtt
      421 catggttcgc catgccgaca tagagcggca caatggcggc agcggaaaac agcgccagtt
      481 gttcgcgtcg ccagtcgtaa tcgcggtaga tgccgttttc atcatcccag aggtaacggt
      541 ttaccgcatc gcgacgggca ctggccttct ggcggaacag cgcttctgtc tctttctcgc
      601 ctttcagcgc cgagatgttg gcgatggcgc tctccagttt aaacaggaag gcattcagat
      661 cgatggggat gaactgggtg gtacgaatgc tcgccagacg accagtatca cgcagccaac
      721 gggaagagta atcccaaccg gaggccgctc ccgcgcgtaa atcgcggtac acctcgttgg
      781 gcgggcgacc agaatgtttc gcggtttcaa cgtcctcaag ccaggattcg tcacgcggcg
      841 tgtcgcgatc gtcccagtaa cggttgagca gcgatccgtc cggcatccgc acaacatggc
      901 gataggcctg attagggatc aacgattctg caccgtccat ccagaaggca tattccattt
      961 taaggtggtc gagatagcgg cgcgcaccgc gtacaccatc ttcttcaaac aactccacca
     1021 tcagcgcaaa aaccggtggt tgcgagcggc tcaaataata ggtgcggttg ccgttgggga
     1081 tgtgaccgta gttttcgatc atccaggcga agttatcggc catgcatttc agcaaatctt
     1141 cccgaccact ttccgccagt cccagcatgg tgaaatagga atcccaatag taggtttcgc
     1201 taaaacggcc gcccgggaca atatatgact gcggtagcgc cagcagagaa gaccacggaa
     1261 tgtgatcctg tggttcgcgg gttagcaccg gccacagctg gtcgatatgc tctttcaggg
     1321 aattttgcgg gtccgataca tactcgctgg agtagacctc cggcagccag aagtgatttt
     1381 caacaaactt gcgcaagtca aaatcacgat gacggcgcac tttacggtag cggattaaaa
     1441 tatccagcgg gtccattttg ggtgcgcagt cgggaaaggt tttactgtcg gggaaaattt
     1501 tcgccgactg aacatgctcg aacagttcga gatagcgatc ggcaggcgtc agcgcatcag
     1561 aggcgggcag cccttcaatc atctcgggtt ccggttctgc ctcgatcatc tcatccagtt
     1621 ttaattcata cgggtccagt tcatagcaga gatcgacctc gatcatcagt tcgtctggat
     1681 tagggttttg aattttctga ttgagcataa ggatccaacc tccgaaattc gtcgctaatt
     1741 atcgggcttt tctttaaggg tagtcattcg ctccgcctgg tgggcgacaa agcgtgcggt
     1801 ggatcacgtt tattaacttg tcaaaaaata taacgcacta atatcaatgg aatttataaa
     1861 aatacggatt tggtgttgtt tttcattttc agaacattca atcctgctct cgccgcaat
//
LOCUS       DAZSNO010000341         1880 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZSNO010000341 DAZSNO010000000
VERSION     DAZSNO010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.346"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(32..870)
                     /gene="aadA1"
                     /locus_tag="ACHWN2_004340"
                     /pseudo
     CDS             complement(32..870)
                     /gene="aadA1"
                     /locus_tag="ACHWN2_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            complement(983..1813)
                     /locus_tag="ACHWN2_004341"
     CDS             complement(983..1813)
                     /locus_tag="ACHWN2_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HFR8933586.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          459 a          411 c          417 g          593 t
ORIGIN      
        1 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
       61 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
      121 aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
      181 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
      241 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
      301 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
      361 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
      421 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
      481 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
      541 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
      601 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
      661 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
      721 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
      781 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
      841 cgatacttcg gcgatcacca cttccctcat gatgtttaac gtttgaaata aggggcgccg
      901 agcgccagcg aggggagcca aaagcttgct tttggccgtc ccgacttgat tgaagggttg
      961 ggcgattttg ccattagatt ttttataaat ttagtgtgtt tagaatggtg atcgcatttt
     1021 tcttggcttt tatgcttgat gttaaattcg accccaagtt tcctgtaagt gcggacacaa
     1081 aaacatattt atgtcctgat ttgcttataa taaacccttc aaaccatccg ttttgtaagg
     1141 ttctatttgc tgtgaatcct gcaccagttt tcccatacag ttttgtacta ttatccagat
     1201 cttgtagata catgttctct atggtgtttt ctatggctga gtttttaact gggagattgt
     1261 gattaataat tttacgcagg aattgaattt gttcttctgg tgaaattttt aagctacttt
     1321 cgagccatgc ttctgttaat ccgttgtttc tttctttatc tccagagaag tcttgatttc
     1381 cataatcaaa atctttgaga taattcttga ttttatttaa tccaattttt tgggttattt
     1441 cttgcgaaac ccaaacaaca gaaaattgca tccacgtctt tggtgtatga ttgctgttcc
     1501 agatctccat tcctttgggg gttttatccc atttgaatat ggttttctga tctattattt
     1561 ccgcatcaaa tgccataagt gataatgcga tcttgaaagt tgaatctggt gccatttgcg
     1621 ttgcacactt tgctttattg aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta
     1681 aaaaacaacc ttcagttcct tcaaataatg gagatgcaac agtagagata tctgttgatg
     1741 cactggcgct gctgtagata atatttgcaa ttattaaaaa aatagcgaag ttgatatgta
     1801 ttgtgttttt cataataagt attggtttgg taaagggctt aattttaacg gctaacaatt
     1861 aatgaggctc cgggttcgcc
//
LOCUS       DAZSNO010000342         1879 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZSNO010000342 DAZSNO010000000
VERSION     DAZSNO010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.347"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..524
                     /locus_tag="ACHWN2_004342"
     CDS             <1..524
                     /locus_tag="ACHWN2_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR8933587.1"
                     /translation="RRSNIINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRV
                     LKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDL
                     RHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPN
                     SSQSKNKEGTNDV"
     gene            873..>1879
                     /locus_tag="ACHWN2_004343"
     CDS             873..>1879
                     /locus_tag="ACHWN2_004343"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR8933588.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     N"
BASE COUNT          582 a          353 c          381 g          563 t
ORIGIN      
        1 taagacgctc gaacatcatc aaccttgaat ggcaacaaat agatatgcag cgccgggtgg
       61 catggataaa cccggaagag agtaaatcaa accgcgcaat tggcgttgcg ctgaatgata
      121 ctgcatgtcg cgtattgaaa aaacaaatcg ggaatcatca ccgttgggta tttgtgtaca
      181 aggaaagctg taccaaacca gacggaacga aagcgccaac ggtcaggaag atgcggtatg
      241 acgcaaacac agcctggaaa gcggcgctga gacgggctgg tattgatgat ttcagatttc
      301 acgacttgag acacacctgg gcaagttggc tggttcaagc cggagtcccg ttatcagtgt
      361 tacaggaaat gggaggctgg gagtctatcg aaatggttcg tcgatatgct caccttgcac
      421 ctaatcacct taccgaacac gcacggcaaa tagactcgat cctgaaccca tcggtcccaa
      481 attcgtccca gtcaaaaaat aaggaaggta ctaatgatgt gtaacttatt gatttaaatg
      541 gtgccgataa taggagtcga acctacgacc ttcgcattac gaatctgtag caccaatcat
      601 aactatctgt tttagcaagc attaaccgca ttcactaagc tatagttgat gacacaaaca
      661 gaaagttgat gcatgatgtt gccatatata tgtcacaaat acggcacaac gatcttcaaa
      721 catatagcca cacattcaca gaagagcaca aagccttgca atccagtgca aagcttggtg
      781 tgtctccgtt ttgtttcatc agttacacat aactatatga tcattaacta aaagtcagta
      841 tacttggaga aattttaatt gcgtaataga gcatgcatat gattgaaatt aacagtttat
      901 tattaataac atccgtgata ttgatgtcgt tattagctgt ggggttattt gataaaattt
      961 ccccaataaa tcttgttgag cacggcagga ataatcaaat agatggaatg cgggggttct
     1021 tagcaatttt cgtgcttatt catcacgcag caatttggaa tggatacttg tcatctggag
     1081 tatgggaagc accttcatca aatctgttag caaatttagg ccaagttggt gtgtcattct
     1141 tttttatgat tactggttat ctgttctttt caaagattat ctctggagat caggactgga
     1201 caaggcttta tgtatcaaga ttactacgat taaccccaat gttcatagtt agcctatgtc
     1261 tcatttttat cattgtaggt tttaagtctg gatggagaat gcaggtatcc acagaagagc
     1321 tttttgtgtc aataatgaag tggttgccat tcactgcgct aggtatgccg aacattaatg
     1381 acgtaaaaga ttcatttact atcaatgccg ctgtaacatg gacacttgta tatgaatggt
     1441 tcttttattt ttctcttccg gtaatttccg cgctcataaa aagaaaagtc agtatttaca
     1501 tggttatgat tagcgcaata tcgctatttg ttttcatttt atttttcagt aaaatacaca
     1561 tagcatcatt tctttttggg ctcctggcat ttttgctaaa taaatcaaag atagtcaatg
     1621 gaattgccaa agcaaaagta acaccaatta taataactgc gataatggtt tttgaaatga
     1681 cgtatttcaa aacaacttac gcaccgctac cactgattct ttgtggaata acatttatca
     1741 ttatcgcatc aggttgtgat ttgtatggaa tattaagatt aaatataacc agaaagctag
     1801 gagaaactac ctacagcgtt taccttctgc atgggatatt cctttattgc ctaatgacgt
     1861 ggattattcc taataatta
//
LOCUS       DAZSNO010000343         1872 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZSNO010000343 DAZSNO010000000
VERSION     DAZSNO010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.348"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..203
                     /locus_tag="ACHWN2_004344"
     CDS             <1..203
                     /locus_tag="ACHWN2_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933589.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="ACHWN2_004345"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="ACHWN2_004345"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR8933590.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          455 a          467 c          443 g          507 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt at
//
LOCUS       DAZSNO010000344         1870 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZSNO010000344 DAZSNO010000000
VERSION     DAZSNO010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.349"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..1870
                     /gene="sat"
                     /locus_tag="ACHWN2_004346"
                     /pseudo
     CDS             1..1870
                     /gene="sat"
                     /locus_tag="ACHWN2_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          688 a          330 c          365 g          487 t
ORIGIN      
        1 aacaaaatac aatgataaac tggttagtga gttaaaagat acctatagtc ataaaataaa
       61 tctgaatggc aataatgtaa ccattaaaaa cacagatata acattacacc aaaacaatgc
      121 agataccact ggtactcaag aaaaaataac taaagacaaa gatattgtgt tcacaaatgg
      181 gggaaatgtt ctgtttaagg ataatttgga ttttggttgc ggtggtatta tctttgacga
      241 aggccatgaa tataacataa acggtcagag atttacattt aaaggagcag gaattgatat
      301 cggaaaagaa agcattgtaa actggaatgc attgtattcc agtgatgatg ttttacacaa
      361 aataggcccc ggtactctga atgttcaaaa aaaacagggg gcaaatataa agataggtga
      421 aggaaatgtt attcttaatg aagaaggaac atttaacaat atataccttg caagcggaaa
      481 tggtaaggta atactaaata aagataattc ccttggcaat gatcaatatg cggggatatt
      541 ttttactaaa cgtggtggca cgctagattt aaatggacac aatcagactt ttactagaat
      601 tgccgccact gacgatggaa caacaataac taactcagat acaaagaaag aagccgttct
      661 ggcaatcaat aacgaagact cctacatata tcatgggaac ataaatggca atataaaact
      721 aacgcacaat attaattctc aggataagaa aactaatgca aaattaattc tggatggtag
      781 tgtcaacaca aaaaatgatg ttgaagtcag taatgccagt cttaccatgt aaggccatgc
      841 aacagagcat gcaatattca gaagcacagc gagtcattgc tccctggtat ttctttgtgg
      901 aacggactgg gtcaccgttt tgaaagaaac agagagttca tataataaaa agttcaattc
      961 tgattacaaa agtaataatc agcagacctc atttgatcag cctgactgga aaaccggggt
     1021 gtttaaattt gatacattac acctgaacaa tgctgacttt tcaatatcac gcaatgccaa
     1081 tgttgaagga aatatatcag caaataaatc agctatcaca atcggcgata aaaatgctta
     1141 cattgataat cttgcaggga aaaatattac taataatggt tttgacttca aacaaactat
     1201 cagtactaat ctatccatag gagaaactaa atttacaggt ggcatcactg cacataacag
     1261 ccaaatagcc ataggtgatc aagctgtagt tacacttaat ggtgcaacct ttctgaataa
     1321 tactcctata agtatagata aaggagcaaa agttatagca caaaattcca tgttcacaac
     1381 aaaaggtatt gatatctccg gtgaactgac tatgatggga atccctgaac agaatagtaa
     1441 aactgtaacg ccgggtctcc actacgctgc tgatggattc aggctgagtg gtggaaatgc
     1501 aaatttcatt gccagaaata tggcatctgt caccggaaat atttatgctg atgatgcagc
     1561 aaccattact ctgggacagc ctgaaactga aacaccgact atatcgtctg cttatcaggc
     1621 atgggcagag actcttttgt atggctttga taccgcctat cgaggcgcaa taacagcccc
     1681 caaagctaca gttagcatga ataatgcgat ctggcatcta aatagccagt catcaattaa
     1741 tcgtctagaa acaaaagaca gtatggtgcg ttttactggt gacaatggga agtttacaac
     1801 ccttacagtg gacaacctta ctatagatga cagtgcattt gtgctgcgtg caaatctggc
     1861 ccaagcagat
//
LOCUS       DAZSNO010000345         1864 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZSNO010000345 DAZSNO010000000
VERSION     DAZSNO010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.350"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            308..1498
                     /locus_tag="ACHWN2_004347"
     CDS             308..1498
                     /locus_tag="ACHWN2_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HFR8933591.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
BASE COUNT          505 a          387 c          441 g          531 t
ORIGIN      
        1 gtaccaccga tagtatcgat atcaaatcag cctgcggccg cgataacttt cgtaacattc
       61 tgccagcggc tttgttgaat aaatcaggtt caagataatc gtctctgcat caggttagag
      121 tttcaggcaa tacatcctct ttctggcata cctgccactc tcgcaccatg gtcatagcct
      181 ttacgatctg catactcacc agaccagagc ccgctccctt ttaggctgag gaatccccag
      241 aaaagaagac aggcatagag ttttgtgatc tactgattgc gctaacaaat tcaatgagat
      301 cacctctatg cctgcccgta aagtatgcca gacttttttc cgtaatgctt tagcttccac
      361 gcatcagtat cgacaaaatg ccattattga ttctgctgct gctttagtcg gtggcgcatc
      421 tctttctctt accagcattg gccggcattt acccggtcct gctcgcgtga aagataaaat
      481 aaaacgcgtt gactgtctgt tgggtaataa acgacttcat aatgatatcc ctctgatatt
      541 caaaaatatt acatccatga tgacaaataa actgtcgtgg tgtgttatcg ctgttgactg
      601 gagcggttac ccttttcagg aatatcatgt ccttcgtgcc agcctcctct gcgacggtcg
      661 ctctatcccc ctgatgagtc aggtcttccc gtcaaaaaaa aataacgagg cggtggaaat
      721 cgcttttctg gatgcgcttt ccggggccat ttctccccgg actcgtgttg ttattgttac
      781 tgatgctggt tttcaaagtg catggttccg tcatatcaaa tcacagggat gggattttat
      841 tggtcgtatc aggggggtag tgaagttcag gctggatagt gacaaagata agtggctgga
      901 cataaaaatg tgcaggggga gttcagaggc aaaatacctg gggacgggaa ccctggcccg
      961 gaaaaaacgc tcgcagtgtg aaggacattt ttatctttat aaacattcac ctaaaggcag
     1021 aaaaagcagg cgtgccagag ggaggccggg cttaccaaca acggaaaaag aacaaaaagc
     1081 cgcaggcaga gaaccatggt taatattcag caatacagct gagtttaatg ccaaaaaaat
     1141 catgaaactg tacagtcgaa gaatgcagat agagcaaaac tttcgggatg aaaaaagtga
     1201 acgctttggt tttggccttc gggcgagcag aagtcggagg ggagagcgtt ttctggtatt
     1261 aagtctactg gttacactgg ccagtatcgt actctggcta cttggttatc attttgaaaa
     1321 taaaggattt catttaaagt atcaggctaa tagtctcaaa aaacgaagag tattgtcttt
     1381 tctgacgctg gctgaaaacg tcctgagatt taaccctgaa cttctacgac gagcacaacc
     1441 tgaaaagata ttgtgccggt tggccagcac ttaccgaagt atggtgctgg cttactgatg
     1501 aaaattttct ggggatccct cagcctttcg gggaggaatg agggctctga tattcttttg
     1561 ccgtagttca tcgtgaaacc gtcggatgtc atttggcctg agtgacccat gcctcccaaa
     1621 agcagagtcg ctagtgttat gtaattaggc ggttaaactt caattctcag tgttaagttt
     1681 gggtaaacgc ttgtaaatgg tattgctaat tttttaattg actaatatta gaaacggtta
     1741 gtgtgattgt atatagatat ttgagtgtaa ggtatttaaa ggggcattca gcccctccgt
     1801 ctatttgtga ttgcatccat tgatgtgtgt attattttga gcttgaaaaa gggaggcgct
     1861 cttt
//
LOCUS       DAZSNO010000346         1861 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZSNO010000346 DAZSNO010000000
VERSION     DAZSNO010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.351"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..345)
                     /locus_tag="ACHWN2_004348"
                     /pseudo
     CDS             complement(1..345)
                     /locus_tag="ACHWN2_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(345..1265)
                     /locus_tag="ACHWN2_004349"
     CDS             complement(345..1265)
                     /locus_tag="ACHWN2_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR8933592.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1403..>1861
                     /gene="pgrR"
                     /locus_tag="ACHWN2_004350"
     CDS             1403..>1861
                     /gene="pgrR"
                     /locus_tag="ACHWN2_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR8933593.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTGNSG"
BASE COUNT          466 a          451 c          433 g          511 t
ORIGIN      
        1 acacacaccc agcacgccaa tgcgttcagc atcaacatat ggcagagtta ctgcataatc
       61 gataacacgg ctaatatcct ctacgcgctg tgtcggatct tctatccaac gaggctaccc
      121 accagattct ccctggaaac tggcgtcata agcgagaacg acatagcctt tttcagccaa
      181 tgccttgcca tagatattgc cagacgtctg ctccttacaa ctgccaaaag gatggacgct
      241 gataatcgcc ggatactgtt ttgactcatc aaaatttggt ggaaactgaa tattggcagc
      301 aatatgccag gccatatcac tattgcggat ttgaatatgt ttcattacag agtttcctca
      361 aagaacggag ctaatactga aattgcttca gccacatagg ctttgccgtc ataaagatcc
      421 atatggtttg cgccttcaac aatgtgatag cgtttatcct ggcttgatgc ccgatcgtac
      481 aggtcgtcac tcatccattt gctccctgcc tggctgcccg ccacaatctg catcggctga
      541 gtcaggtaca cttccgccat atggtaagca tcataggtaa taatctggtt aaggctgcgc
      601 aaagtagcgt aacccggtgc tgttggatac tgcgcacgag gtgtgtggta atactcccag
      661 gcctgacgca gttcttcgtt cggcgcatcg gcctccttca ttggagccag tggcataatg
      721 gcgtattctt cgctgctaat atcgctggtt cgggcgtttg agccagcttc aatgtgtggc
      781 agagcatcaa tagatttcac attgttttcc caaccattgc ggaacatcga accaatattg
      841 accgcactaa cggtaccgat ggccttgatg cggcgatcct gaattgcagc attggctgta
      901 tatcctgcac cggcacaaat tcccatcgca ccaattcggg tattgtcgac ataagaaagc
      961 gttgtcaggt aatcaatcac ggcactgacg tcttcagtac gaatgtatgg gttttctaac
     1021 tgacgcggct cgccgccact ttcaccttga taagatgcgt cataagcaat agtgacaaat
     1081 cccttttccg ccagcttttc ggcataggtt ccggccgttt gctctttaac gcccccacct
     1141 gggtgagata ccacaattgc cggatactga cgggtttcat caaattttgg agggaaatag
     1201 atcactgcag acaaagagat ggttggatta ttgctgttag tgaagctgac tttattattc
     1261 atcattcagt tcctcatgga gttgtcggtt cgttttgacg gttggtgata tcactataga
     1321 tattgatcat taagttgatt agaccctaaa tcatgattag acctatcatt taaatgatta
     1381 atagattgta tggagatact gaatgaagcg tgaagaaatc gctgatctga tggcgtttgt
     1441 cgtcgttgca gaggagcgta gcttcactcg cgcagcagcc cgcctgagca tggtgcagtc
     1501 agctttaagc cagatagtgc gccgtataga agaacgattg ggattgcggc ttctgacgcg
     1561 aaccacgcgc agcgttgtac ccactgaagc gggcgagcat cttttgtctg ttcttggccc
     1621 tatattgcat gacatagatt ctgccctgac atccctgagc gatctgcaga accgcccatc
     1681 cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt taccagccat
     1741 gcgcacattc ctgaaatcgc atcctgaaat tgatattcag cttaccattg attatggttt
     1801 gaccgatgtc gtttctgaac gttttgatgc aggcgtcctg aggtgtactg gcaatagcgg
     1861 a
//
LOCUS       DAZSNO010000347         1857 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZSNO010000347 DAZSNO010000000
VERSION     DAZSNO010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.352"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(43..192)
                     /locus_tag="ACHWN2_004351"
                     /pseudo
     CDS             complement(43..192)
                     /locus_tag="ACHWN2_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            268..>1857
                     /gene="ospD3"
                     /locus_tag="ACHWN2_004352"
     CDS             268..>1857
                     /gene="ospD3"
                     /locus_tag="ACHWN2_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR8933594.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTY"
BASE COUNT          621 a          341 c          363 g          532 t
ORIGIN      
        1 ttgtcagtat ggctcaatgt gataatagtt ggaaagtttg atgggtttcg ccccgttgta
       61 gcggtagtcg aactggaagg tcttcaggca ctgcttacag cgatagagca gcccccctga
      121 actggaatgg ccgttccgga tacccccctg agtttcagag taacggtgac aaaccacatc
      181 aatctttgcc atcaatcatc caaagggcaa agagtacaac aacactaagt ctgcgtcaca
      241 acccatcaat gaaaggaata tatacatatg ccatcagtaa atttaatccc atcaaggaaa
      301 atatgtttgc aaaatatgat aaataaagac aacgtctctg ttgagacaat ccagtctcta
      361 ttgcactcaa aacaattgcc atatttttct gacaagagga gttttttatt aaatctaaat
      421 tgccaagtta ccgatcactc tggaagactt attgtctgtc gacatttagc ttcctactgg
      481 atagcacagt ttaacaaaag tagtggtcac gtggattatc atcactttgc ttttccggat
      541 gaaattaaaa attatgtttc agtgagtgaa gaagaaaagg ctattaatgt gcctgctatt
      601 atttattttg ttgaaaacgg ttcatgggga gatattattt tttatatttt caatgaaatg
      661 atttttcatt ccgaaaaaag cagagcacta gaaataagta catcaaatca caatatggca
      721 ttaggcttga agattaaaga aactaaaaat gggggggatt ttgtcattca gctttatgat
      781 cccaaccata cagcaactca tttacgagca gagtttaaca aatttaactt agctaaaata
      841 aaaaaactga ctgtagataa ttttcttgat gaaaaacatc agaaatgtta tggtcttata
      901 tccgacggta tgtctatatt tgtggacaga catactccaa caagcatgtc ctccataatc
      961 agatggcctg ataatttact tcaccccaaa gttatttatc acgcgatgcg tatgggattg
     1021 actgagctaa tccaaaaagt aacaagagtc gtacaactat ctgacctttc agacaatacg
     1081 ttagaattac ttttggcagc caaaaatgac gatggtttgt caggattgct tttagcttta
     1141 caaaatgggc attcagatac aatcttagca tacggagaac tcttggaaac ttctggactt
     1201 aaccttgata aaacggtaga actactaact gcggaaggaa tgggaggacg aatatcgggt
     1261 ttatcccaag cacttcaaaa tgggcatgca gaaactatca aaacatacgg aaggcttctc
     1321 aagaagagag caataaatat cgaatacaat aagctgaaaa atttgctgac cgcttattat
     1381 tatgatgaag tacacagaca gatacccgga ctaatgtttg ctcttcaaaa tggacatgca
     1441 gatgctatac gcgcatacgg tgagctcatt cttagccccc ctctcctcaa ctcagaggat
     1501 attgtaaatt tgctggcctc aaggagatat gacaatgttc ccggacttct gttagcattg
     1561 aataatggac aggctgatgc aatcttagct tatggtgata tcttgaatga ggcaaaactt
     1621 aacttggata aaaaagcaga gctgttagaa gcgaaagatt ctaatggttt atctggattg
     1681 tttgtagcct tgcataatgg atgtgtagaa acaattattg cttatgggaa aatacttcac
     1741 actgcagacc ttactccaca tcaggcatca aaattactgg cagcagaagg cccaaatggg
     1801 gtatctggat taattatagc ttttcaaaat aggaattttg aggcaataaa aacttat
//
LOCUS       DAZSNO010000348         1851 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZSNO010000348 DAZSNO010000000
VERSION     DAZSNO010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.353"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..249)
                     /locus_tag="ACHWN2_004353"
     CDS             complement(<1..249)
                     /locus_tag="ACHWN2_004353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933595.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEI"
     gene            complement(1002..1400)
                     /locus_tag="ACHWN2_004354"
                     /pseudo
     CDS             complement(1002..1400)
                     /locus_tag="ACHWN2_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
     gene            complement(1403..1769)
                     /locus_tag="ACHWN2_004355"
                     /pseudo
     CDS             complement(1403..1769)
                     /locus_tag="ACHWN2_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          425 a          516 c          437 g          473 t
ORIGIN      
        1 aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt ttttgtttcc
       61 ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat cgtcaaaatt
      121 atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt gaagttttac
      181 gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg gtttagctga
      241 acgggccata taactacctg aaagacaatg atattgtctg tttttataac ggtaatcaca
      301 ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa cgcttattta
      361 gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat tgtccttatt
      421 ccactaatac aaactttttg taaagtatat taaatatact tcatttcata ctgaaatccc
      481 cggaagtatt caatcaaaca attacaccat aaaaggaaga gggggcccca attaagttgt
      541 caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca ttggtactct
      601 ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca ggtccacgtt
      661 ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc gcaggcagta
      721 aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg ctttcgacac
      781 atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc tcacgtccga
      841 ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg atggtttggt
      901 gcctgcgcct gcacgccgac accaaggggc cttccctcat ctcttgtgct gcatggctgc
      961 acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc atagcaacag
     1021 taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg gcctcgctgg
     1081 atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta gcgtttattc
     1141 agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat cacaccgccc
     1201 tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg ttttccacag
     1261 cagtctcata aaacgtgtat cactgatcct gcggcagacg gctttcatca gcagccggtg
     1321 atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc ccgtgtttcc
     1381 ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg ttcgggccac
     1441 tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt gcacggtaac
     1501 tgatcgggct gaacttctgg gaggcttttg cgctcagttc ctgacggcgc agactggctg
     1561 ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca ggcgtggggc
     1621 accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg ctgacggaac
     1681 tgcggacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc gctgcgggcc
     1741 acccggagta cgcggcacat tgccttgatg acggtttacg cggtcgcctg cggcaacggc
     1801 tttccggctg acggtagtgt ggaccttttt accccggaga acaccggcaa g
//
LOCUS       DAZSNO010000349         1850 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZSNO010000349 DAZSNO010000000
VERSION     DAZSNO010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.354"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..258)
                     /gene="ydfV"
                     /locus_tag="ACHWN2_004356"
     CDS             complement(<1..258)
                     /gene="ydfV"
                     /locus_tag="ACHWN2_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFR8933596.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            283..522
                     /gene="relB"
                     /locus_tag="ACHWN2_004357"
     CDS             283..522
                     /gene="relB"
                     /locus_tag="ACHWN2_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFR8933597.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            522..809
                     /gene="relE"
                     /locus_tag="ACHWN2_004358"
     CDS             522..809
                     /gene="relE"
                     /locus_tag="ACHWN2_004358"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFR8933598.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            881..1036
                     /gene="hokD"
                     /locus_tag="ACHWN2_004359"
     CDS             881..1036
                     /gene="hokD"
                     /locus_tag="ACHWN2_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR8933599.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1254..1505
                     /gene="rem"
                     /locus_tag="ACHWN2_004360"
     CDS             1254..1505
                     /gene="rem"
                     /locus_tag="ACHWN2_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFR8933600.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1572..1850
                     /locus_tag="ACHWN2_004361"
                     /pseudo
     CDS             1572..1850
                     /locus_tag="ACHWN2_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          410 c          468 g          465 t
ORIGIN      
        1 tgacatgcta aagcaaaaaa tagatgaata agttgagttg tgcatatgta gcctgaccgt
       61 cacaaagtat atggtgtctg taccagtaag atgatggccg gactctttaa aaaagagctg
      121 acctgcacaa tacaggatgg acttagcaat ggctgctcct ggcacaaagc ggacagtgat
      181 caccgttctt acgactactt tctgacttcc ttcgtgactt gccctaagca tgttgtagtg
      241 cgatacttgt aatgacattt gtaattacaa gaggtgtaag acatgggtag cattaacctg
      301 cgtattgacg atgaacttaa agcgcgttct tacgccgcgc ttgaaaaaat gggtgtaact
      361 ccttctgaag cgcttcgtct catgctcgag tatatcgctg acaatgaacg cttgccgttc
      421 aaacagacac tcctgagtga tgaagatgct gaacttgtgg agatagtgaa agaacggctt
      481 cgtaatccta agccagtacg tgtgacgctg gatgaactct gatggcgtat tttctggatt
      541 ttgacgagcg ggcactaaag gaatggcgaa agctgggctc gacggtacgt gaacagttga
      601 aaaagaagct ggttgaagta cttgagtcac cccggattga agcaaacaag ctccgtggta
      661 tgcctgattg ttacaagatt aagctccggt cttcaggcta tcgccttgta taccaggtta
      721 tagacgagaa agttgtcgtt ttcgtgattt ctgttgggaa aagagaacgc tcggaagtat
      781 atagcgaggc ggtcaaacgc attctctgaa ccaaagcatg acatctctgt ttcgcaccga
      841 aggtgacact tctgctttgc gttgacagga gaagcaggct atgaagcagc aaaaggcgat
      901 gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg acggcactgg taacgaggaa
      961 agacctctgc gaggtacgaa tccgaaccgg ccagacggag gtcgctgtct tcacagctta
     1021 cgaacctgag gagtaagaga cccggcgagt gagaaatccc tcgccacctc tgatgtggca
     1081 ggcatcctca acgcacccgc acttaacccg cttcggcggg tttttgtttt tattttcaac
     1141 gcatttgaag ttctggatgg tgccggaata gaatcaaaaa tacttaagta gcgcgcaggg
     1201 ataaaaggga tggaccccga acaggggagt gctacttatc tggaaggatt ctgatgatga
     1261 acatcgaaga actgcgtaaa attttttgtg aagatggcct ctatgctgtg tgcgttgaaa
     1321 atggaaatat tgttagtcat taccgcattg tgtgtttgca aaaaaatggg gctgcgttaa
     1381 ttaattttgt ggatgcccga gtgacggacg gatttatctt gcgcgacggt gagtttgtca
     1441 cttcattaca ggcattgaaa gagatcggaa taaaagctgg cttttctgct ttttcagaag
     1501 aataaactca tctacaatct tgcgcggggc tgaactcccg ctgagtaaca ccgtgccacc
     1561 ggagaaaacc gatggcacgc aacgcaaaat attacaatca tgataattcg accgttcttg
     1621 cccacacgca cgagcggtat tctcacgcat ttaagtcaga ctggtaccag catccctcat
     1681 gcactgaaga acaggccgaa tggcttattc agtgttaccg caggcgcgga tgcgaggtta
     1741 aaaaagccct tagcctcgac taccgtcact agataatctc cgtcaggctc ccttactccg
     1801 aacagccacc gcgtccgtcc cgcacattcc agcaacggat ctggaggtaa
//
LOCUS       DAZSNO010000350         1832 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZSNO010000350 DAZSNO010000000
VERSION     DAZSNO010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.355"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..180)
                     /locus_tag="ACHWN2_004362"
     CDS             complement(<1..180)
                     /locus_tag="ACHWN2_004362"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933601.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="ACHWN2_004363"
     CDS             complement(321..875)
                     /locus_tag="ACHWN2_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR8933602.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="ACHWN2_004364"
     CDS             complement(872..1162)
                     /locus_tag="ACHWN2_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR8933603.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQVAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..1761)
                     /locus_tag="ACHWN2_004365"
     CDS             complement(1162..1761)
                     /locus_tag="ACHWN2_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR8933604.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          446 a          496 c          420 g          470 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cgcatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctacctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcaaaaaa tcgcaactct ccggcgtcgc cgggagaagt aatccggaag aggtttgttt
     1741 gaccagttgt atatgcgcca tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc
     1801 cgttatgtag tataccagaa ttaatgccaa ta
//
LOCUS       DAZSNO010000351         1829 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZSNO010000351 DAZSNO010000000
VERSION     DAZSNO010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.356"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..362)
                     /locus_tag="ACHWN2_004366"
     CDS             complement(<1..362)
                     /locus_tag="ACHWN2_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8933605.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHWN2_004367"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHWN2_004367"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFR8933606.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          547 a          472 c          377 g          433 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttgaat
//
LOCUS       DAZSNO010000352         1812 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZSNO010000352 DAZSNO010000000
VERSION     DAZSNO010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.357"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(109..183)
                     /locus_tag="ACHWN2_004368"
     tRNA            complement(109..183)
                     /locus_tag="ACHWN2_004368"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(148..150),aa:Gly,seq:tcc)
     gene            complement(187..262)
                     /locus_tag="ACHWN2_004369"
     tRNA            complement(187..262)
                     /locus_tag="ACHWN2_004369"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(265..341)
                     /locus_tag="ACHWN2_004370"
     tRNA            complement(265..341)
                     /locus_tag="ACHWN2_004370"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(305..307),aa:Arg,seq:tct)
     gene            complement(343..418)
                     /locus_tag="ACHWN2_004371"
     tRNA            complement(343..418)
                     /locus_tag="ACHWN2_004371"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(383..385),aa:Ile,seq:cat)
     gene            complement(595..1284)
                     /locus_tag="ACHWN2_004372"
     CDS             complement(595..1284)
                     /locus_tag="ACHWN2_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR8933607.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1281..1640)
                     /locus_tag="ACHWN2_004373"
     CDS             complement(1281..1640)
                     /locus_tag="ACHWN2_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR8933608.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1640..>1812)
                     /locus_tag="ACHWN2_004374"
     CDS             complement(1640..>1812)
                     /locus_tag="ACHWN2_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933609.1"
                     /translation="KECPLNSGYLNMVPALRELMQAKHRAQKAVDELVDCVAELETRV
                     GKFEKLVQEVLR"
BASE COUNT          445 a          507 c          389 g          471 t
ORIGIN      
        1 actggaactg caacaaaaca acatcacaga acaggagatg gtaaggagtc agacattccc
       61 acacaatatt gtgtcagtgc aaataacaac cttcgtctca aatctcgctg gagcgggcag
      121 cgggaatcga acccgcatca tcagcttgga aggctgaggt aatagccatt atacgatgcc
      181 cgcatatggt gccgactacc ggaatcgaac tggtgaccta ctgattacaa gtcagttgct
      241 ctgcctgctg agctaagtcg gcgctggccc accaccgagg actcgaacct cggaccgtca
      301 acttagaagg ttgatgctct atccggatga gctagtggtg tttggtggcc cttgctggac
      361 ttgaaccagc gacctggcga ttatgagtcg ctcgctctca ccactgagct aaagggccgg
      421 aggcagaata ataaccatat gtcatcacat ctgcaaactc atctgaccac cagcacgttt
      481 aacgtcctgt gccatttttc aggcataaaa aaaccgctcg gcggcgggtt ttgcttactt
      541 taccatcgcg tacaaaatcg gcaaagtatc agatttgcat gagatatatg ccttttaatc
      601 tacttttgca atactttgct ttgaaaatgc cgctttttgt tctgaacgtg ttctcatcac
      661 aaacaataaa gcctcactat ccagtcggtg aaaaatgtgt ttcattacaa cccagtgacc
      721 agtaaatgtt ttggaccagt ttttggttgt tactcccgcc agtaatgcca gctcctgata
      781 ttcataacct tccccaccaa aaagttctgc ttttaccgcc tgcgccgcca gccagattaa
      841 ttttttcagg cgttcctgtg ttttctctgc aattttccta gtgccagaat gagccttaaa
      901 ctcttcccac gcccagtggg ttatggtgat ctgatactcc cagcaaatac ttccgctgta
      961 acaccacaac aaccaggctt tatagtgctc ttcaagagac agaacagccc gccgccacga
     1021 tgatgtcgaa aacgcaacct gactgaccag aggaattgac gcccccttcg ccagtgattg
     1081 ttttcccggg attggtggat tatcccgcgt tatcattttt ccggtcacct catcgcggta
     1141 ccggattttt ttacgcctgt aacgccctgt atcgaacatg gcattctcct gccaggcttc
     1201 aagctgacct tttgtcgccc cactcaaatc agcggtggcg ataatgagct gctcacgaac
     1261 aaactgtaaa tactggttat tcatgcacac tccagctctg tgatttttat ccccaaccga
     1321 ccaccaggaa caggcagtcc gcgcacaata ttggtttcat caaactgctc gtcgtcgata
     1381 agcaaccccg catgtgtcag tgcatccagt ggtgctttca ggatattgtc caggtcacga
     1441 cgccgcttat ccggtggctc tgcaataatc tttatcgcca gtcttccgga caggcttaat
     1501 ttcagtcgct gctgacggac aataagcgcc acatcccggc gataacgctc accggctttt
     1561 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1621 aaaataaact catggcgcat cagcgcagca cctcctgcac cagcttttca aactttccga
     1681 ctctggtttc cagctctgcc acacagtcca ccagctcatc tactgctttc tgtgcgcgat
     1741 gcttcgcctg catcagttcc cgaagcgcgg gtaccatatt gaggtagcct gagtttaacg
     1801 gacactcctt cc
//
LOCUS       DAZSNO010000353         1809 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZSNO010000353 DAZSNO010000000
VERSION     DAZSNO010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.358"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            173..1198
                     /locus_tag="ACHWN2_004375"
     CDS             173..1198
                     /locus_tag="ACHWN2_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR8933610.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="ACHWN2_004376"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="ACHWN2_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAZSNO010000354         1779 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZSNO010000354 DAZSNO010000000
VERSION     DAZSNO010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.359"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..360
                     /locus_tag="ACHWN2_004377"
     CDS             <1..360
                     /locus_tag="ACHWN2_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR8933611.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="ACHWN2_004378"
     CDS             361..726
                     /locus_tag="ACHWN2_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR8933612.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1411
                     /locus_tag="ACHWN2_004379"
                     /pseudo
     CDS             723..1411
                     /locus_tag="ACHWN2_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            1605..1680
                     /locus_tag="ACHWN2_004380"
     tRNA            1605..1680
                     /locus_tag="ACHWN2_004380"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="ACHWN2_004381"
     tRNA            1682..1758
                     /locus_tag="ACHWN2_004381"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
BASE COUNT          490 a          376 c          492 g          421 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtagg
//
LOCUS       DAZSNO010000355         1759 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZSNO010000355 DAZSNO010000000
VERSION     DAZSNO010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.360"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..263)
                     /locus_tag="ACHWN2_004382"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="ACHWN2_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="ACHWN2_004383"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="ACHWN2_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR8933613.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1163..1348)
                     /locus_tag="ACHWN2_004384"
     CDS             complement(1163..1348)
                     /locus_tag="ACHWN2_004384"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR8933614.1"
                     /translation="MLPLSVNLMSSGASLAARLQQHWLWYSYNTKTGGVLAYTFGPRN
                     DETCRELLVLFTPFCIY"
     gene            complement(1373..1645)
                     /locus_tag="ACHWN2_004385"
     CDS             complement(1373..1645)
                     /locus_tag="ACHWN2_004385"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFR8933615.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
BASE COUNT          502 a          433 c          319 g          505 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacga ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgaggttc
     1741 ttttcgccag tcctgtaat
//
LOCUS       DAZSNO010000356         1747 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZSNO010000356 DAZSNO010000000
VERSION     DAZSNO010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.361"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            454..1302
                     /locus_tag="ACHWN2_004386"
     CDS             454..1302
                     /locus_tag="ACHWN2_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR8933616.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1319..1600
                     /locus_tag="ACHWN2_004387"
                     /pseudo
     CDS             1319..1600
                     /locus_tag="ACHWN2_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
     gene            1636..>1747
                     /locus_tag="ACHWN2_004388"
     CDS             1636..>1747
                     /locus_tag="ACHWN2_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933617.1"
                     /translation="MAGIRSTLIKEKNMNTNYEATVATTDNIVHEVYLEGK"
BASE COUNT          499 a          398 c          411 g          439 t
ORIGIN      
        1 agcattatat agttctactc tggcgagata cgttggcata ttcatttcct tactggttgt
       61 gtgagaactt cagtaaagat accaccaaag cccggaagtg gtgaaataaa accgggcaca
      121 acacgaaggc gcatttccgg tatccataaa gagtcggtct tgtctgtgaa atttaaatgg
      181 tgggagtgcg cttccggttg taaataacga cattgctgtg tgaagtacca gttggcggca
      241 tcggtttaat tgctggctga tgtccgccct ttttaaagtg aattttgtga tgcggtgaat
      301 gcggctatgc gcacgcggca cagttaaaag tcatgttagt ccttattggt ttgggtggga
      361 aagccaactg taattgttaa ctggttaacg tcacctggag gcaccaggca ccgcatcaac
      421 aaagttcact tcggtgatga aaggaaagag aaaatgttga atgtagctat tgaaaaccag
      481 aacgggtgga attatagtgc acctgcacct cataaaacgg gtgccgggat tgctaccccg
      541 acgatgacta ctgcgcataa ccgcgctcag gcggtttttt tatgcgtcaa gcacagccac
      601 attcagatta tggtggggcg tgcagggcag ccgcaaggct ggccggtttc ggtagtcacc
      661 ggttgtagca accctgtgcg tctcaccacc catgagattg ctacctctgg tggtgagtca
      721 tttaaactga ctatcgaggc tgccatcatg gctactatcc ttaccctttc tcaccctgac
      781 gcaaccatcg aaaatggtcg cgctgtcact acgtctgttg cggttgcgga gttcttccgg
      841 aaaatgcaca aaaacgttat tcagaagatc gaaactctcg aatgctcgcc ggagtttaac
      901 cggcttaatt ttaagcccgt cacctatacc gacgccaaag gcgaaaaacg cccaatgtac
      961 caaatcacca aaaacggctt cgttttcctg gtgatgggct tcactggcaa aaaagccgcc
     1021 gcattcaagg aagcctacat tgctgagttc gaccgcatgg aggccgaact gcgccagaat
     1081 aacaccccac ctgcagacaa gatgattccg ggtgatggtc gcactctggt tgttcacttc
     1141 gacaaattcg gcaatgtcga attcaccgaa accgttcctg atggcgcact ggtctgtacc
     1201 ctggagactt tccggtttta tctggaaaaa cagggatgga ctcttgttaa ccggggcgca
     1261 attaaaaata tgactgtgga acaattactt tctttaaaat aaaaatggag ctacggatat
     1321 gaaccctatg cttgattatt gttttacctc tgcacctcag cagaccataa cacacaccgg
     1381 aatcaaaaat ttgttatgga taatcaaaaa aaacctccag tagaaaggtg atcccgattt
     1441 aattccctgt gcttatggcc tgtgtgacag acaagtaagc cctgataagg cttacactgc
     1501 tgaacttatt tacatgcacg gaaccatgat gaggaaaaag aaacgcaaat attgtagtga
     1561 aatctgcgcc gaaaaagacc agatggcaca cgaactttaa ttaactgact attcgaaact
     1621 gaatttatgc cagcaatggc agggattcgc tcaaccttaa ttaaggagaa aaacatgaac
     1681 accaattatg aagccactgt tgcaactact gataacattg ttcacgaggt ttacctggaa
     1741 ggaaagc
//
LOCUS       DAZSNO010000357         1733 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZSNO010000357 DAZSNO010000000
VERSION     DAZSNO010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.362"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(9..218)
                     /locus_tag="ACHWN2_004389"
                     /pseudo
     CDS             complement(9..218)
                     /locus_tag="ACHWN2_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(268..519)
                     /locus_tag="ACHWN2_004390"
     CDS             complement(268..519)
                     /locus_tag="ACHWN2_004390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933618.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            971..1255
                     /locus_tag="ACHWN2_004391"
     CDS             971..1255
                     /locus_tag="ACHWN2_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFR8933619.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            1255..1530
                     /locus_tag="ACHWN2_004392"
     CDS             1255..1530
                     /locus_tag="ACHWN2_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR8933620.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          475 a          435 c          408 g          415 t
ORIGIN      
        1 ctcactcctt atttacccgg gaaatacttc tccagcaatg catacaggat ggcagcggtt
       61 tcagcatcca ttatgccgtc ataatttttc tgccggaaat gaagctgaaa agcgcggaca
      121 agcgccttat atccgtcatc cgtctccaca ccagaaatac tgtatccata ggtcttcagc
      181 cgtgacacga tatcaacctt atccggaatg ccctgcgatt caaaaaataa atatttagtc
      241 agtaatttta tgtttttcct gagaaaatta ctctttttcg aactccagtg agcgttcacg
      301 actcatcagt gtgaaaatga tggcaaaaac agcagacagt atcagtacga taaatgtggc
      361 cccccatccc agataatcga caacaagtcc cattcccagt gtcgcaacaa ttgagccgaa
      421 gatataggaa aatgtgcctg acagccctaa cagaataagt ttacgcgtaa atcgccctga
      481 atccagaaga gcattaagta tcacataaac aggttccata ccagtcctga ccgcatcagg
      541 acgccagtaa ataaaatctc gtagtccacc ggcatcagag acaagagtaa tcgtcgttac
      601 cggtatttcg actggtttgt actggtgtga gcggacaaaa ctactgtacg tctctcatac
      661 acgctcgtaa gcggccctca cggcccgcta acgcggagat acgccccgac tgcgggtaaa
      721 cccttgtcgg gaccactccg accgcgcaca gaggcatttt catcgctgac aggccgcatg
      781 gcctcgcaga gccggagggg gactggtgat attcggacga ccgtaccggc ttgcgccggg
      841 cttcggcggt tttacacctg tgctacatgt aaccaccgta tgccgcctta tatgcgctga
      901 ctcgcattat ttgccttgtt ccgtctgaaa gtaatcacta tgattaaata tgattaacaa
      961 ctaatcgggt atgcaaatga aaaacaatac cgcacaagca acaaaagtaa ttaccgcgca
     1021 tgtgccatta cctatggctg ataaagtcga ccagatggcc gccagactgg aacgctcccg
     1081 gggctgggtt atcaaacagg cgctttctgc atggcttgcc caagaggagg agcgtaatcg
     1141 cctgacgctg gaagccctgg acgatgtgac atccggacag gttatcgacc atcaggctgt
     1201 acagtcctgg gcggacagcc tcagtactga ccatctgtta ccggtgccac gctgatggaa
     1261 ctgaagtgga ccagtaaggc actttctgat ttggcgcggt tatatgattt tctggtgctg
     1321 gccagtaaac ctgctgccgc cagaacggtg cagtccctga cacaggcgcc ggtcattctg
     1381 ttaactcatc cacgtatggg agaacagttg tttcagtttg aacccaggga ggtcagacgg
     1441 atttttactg gcgagtacga aatccgttac gaacttactg gccagactat ttatgtattg
     1501 cgcctgtggc acacacgaga gaacaggtag attgggtgtg gaggcaactt taacatagcc
     1561 tgcaggtatt aattctggag cattgttatc aggccagata ttttgcattc cattaaataa
     1621 tcttgtagga gataaaataa attacgatga aataacgaaa attaccgctc gtgactggcg
     1681 gcaatatcgc gctccgggct ggcaaataac ccatcagaaa cgttattgcc aga
//
LOCUS       DAZSNO010000358         1729 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZSNO010000358 DAZSNO010000000
VERSION     DAZSNO010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.363"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..388)
                     /gene="istB"
                     /locus_tag="ACHWN2_004393"
     CDS             complement(<1..388)
                     /gene="istB"
                     /locus_tag="ACHWN2_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFR8933621.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVR"
     gene            complement(385..741)
                     /locus_tag="ACHWN2_004394"
                     /pseudo
     CDS             complement(385..741)
                     /locus_tag="ACHWN2_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            821..>1729
                     /locus_tag="ACHWN2_004395"
     CDS             821..>1729
                     /locus_tag="ACHWN2_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFR8933622.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          446 a          400 c          429 g          454 t
ORIGIN      
        1 cacggactgc ttcatagccc atcgctattg ccagatgggt tttccccaca cctgatggcc
       61 ccagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac gactggagtt
      121 gcttctgcgg tgctccggtg gcgaatgtga agtcatactc ttcgaacgtt ttcaccgccg
      181 ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc agtttttctt
      241 catgaagcag atgctccagg aagtccatat aactccattc ctggtctact gcctgttgtg
      301 acagcgcagg cgctgcgctt ataaggcttt ccagttgcaa ctgcccggcg agcgccatca
      361 gtcgttgatg ttgcagttcc atcatcacgc cactcctctg cagaatgagt cgtagatgga
      421 gagtggatga tgctgggggg ggttgtcgaa gctcaccaga ttttcaccag gatgcacgtc
      481 atactctttt ttctccggag gcagtgccag catggactgc tgctcttcga gccagcgatc
      541 acagggacgg gcctggattg tttcatgctt tcgttggtta gcgacatcgt gcagccagcg
      601 cagaccgtgg cggttggctg tttcaacatc gacagtgctc cccatcgggc gcaggcgagt
      661 cattagtggg atgtaaaaac tgttacgggt gtactgcacc atccgttcca ccttaccttt
      721 agtctgtgcc ctgaagggct gaggaatccc cagaaaacga gacaaacata gcattatgtg
      781 atctactgat ttgtgccaac aattcaatga gatcactgct atgcctgttc ataaagtatg
      841 tcagaatttc ttccgggatg ccttagcacc atttcatcag taccgacaaa atgccctgat
      901 ggatgccaca atggcgttaa tcaacggagc atctctgacg cagaccagta tcggacgttt
      961 tttgccgggt aatgcacaag ttaagaataa gatcaaacgt attgatcgcc tcatgggtaa
     1021 tgaggcgctc catcgcgata ttccaatgat tttcaggaat atcacttcaa tgctgacacg
     1081 gcagttatct ttatgtgtaa tagccgtaga ctggagtggc tatccctccc aggagcatca
     1141 tgttcttcgt gccagccttc tgtgtgacgg gcgttccatt ccgttattga gtaaggttgt
     1201 tccttctgaa aaacaaaata atccattgat acaacatgat ttccttgatt cccttgctca
     1261 atccctgccc cccgatgcca gggttattat tgtcacggat gctggatttc agagcgcatg
     1321 gtttcaccat attacttctc tgggctggga ttttattggt cggatcagaa ataacgttca
     1381 gtattgcctc gataacgcgc ctgaaagatg gttaaaagtg tcggatagcc ctgaatgtaa
     1441 aacaccggaa tatatggggg ctggaaggct ggttaaagaa aggaaaaaaa gcatccgggg
     1501 gcatttttac acctataaga aatcggcaaa agggcgaaaa aagaaacgtt cgaaaggtca
     1561 gagtggttta aacaaaacag acaaagaaca gagcaaatca gcaaaagaag cctggttgat
     1621 cttcagcagt acaaatgact tcagagcgcg agaaatcatc aagttataca gtcggcgaat
     1681 gcagatagag caaaactttc gtgatgaaaa gaacgggcgc ttcggattt
//
LOCUS       DAZSNO010000359         1721 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZSNO010000359 DAZSNO010000000
VERSION     DAZSNO010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.364"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..679)
                     /gene="spa29"
                     /locus_tag="ACHWN2_004396"
                     /gene_synonym="spaR"
     CDS             complement(<1..679)
                     /gene="spa29"
                     /locus_tag="ACHWN2_004396"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR8933623.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIF"
     gene            complement(679..939)
                     /gene="spaQ"
                     /locus_tag="ACHWN2_004397"
     CDS             complement(679..939)
                     /gene="spaQ"
                     /locus_tag="ACHWN2_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFR8933624.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            complement(954..1604)
                     /gene="spa24"
                     /locus_tag="ACHWN2_004398"
                     /gene_synonym="spaP"
     CDS             complement(954..1604)
                     /gene="spa24"
                     /locus_tag="ACHWN2_004398"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFR8933625.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
BASE COUNT          695 a          330 c          261 g          435 t
ORIGIN      
        1 taaatattgc aagtaaactt ttaatagtta atgatacgga aaaagcattc atctgcggag
       61 caaatctcga taatacacca agtaatactt ctgatagtaa caatactatc ataacaggac
      121 tggctaaaat aacagcctga cttgccaata aagtcagaaa tgttaagata tttgagacac
      181 ggaaagaaca ttgagaaaat aacgggcata tattataaga caattgtatg gattctaaaa
      241 taaagaccat accaccactg tatagaaata caactgcaga aaaaagattg aaaaattttg
      301 ccaactcaga cgtatcaaca ccattggcag gatcaattga actactaagc gttgccccac
      361 gctggttgtc aataatgcta ccaacagcat gaaatatcca aaagggaaga gacaagcaaa
      421 aagagagaag gaggccaaca attatttcct tgaacataag gaaataaaca tgttcaaaaa
      481 cagaagaacc tatgtctacc ttaccagaag taattaatcc tgatgcaaca agaaaaatca
      541 caggtattct aatagatgga gaaattatac cgttatttaa aaatggaaga aagaaaaata
      601 atggagccaa tctaaaaaaa acgccgttca ggagtattaa aaacacatga atactttcga
      661 accagcttga aatgtccatc aaaccccact cttaattaaa aacattattt catgacaaaa
      721 agacaataaa acctcaccat accatccaga aagaagtagc aaagatattg agacacctat
      781 aagctttata ccaaaaggaa gtgtctgctc ttggagttga gtcactgtct gtaacaaacc
      841 aatagataat ccaataaccg tagctatacc tactggccat aaggaaaaga taaggattaa
      901 ataaagagcc ttattaccca tataaactat atcagacaca ttacctccat catctaagca
      961 ggaatattga tatattgttc aatcaaggcc ttagacagga taccccaccc atcaagcgca
     1021 acaaataata caagttttat tggtactgat attgtgattg gactcatcat catcatacca
     1081 agtgcaagta aaatgcttga aataacaaga tcaacaacaa caaatggcaa atataaataa
     1141 aaacctattt tgaacgcatc tttgatttca cttagcgcat atgcaggcaa gagggaaaac
     1201 aatgaataat cattattctc tgcactcttt aaatcagcat tttcttcttc agacctttgg
     1261 aaaaaacgtg ctaactcaag atctgtatgc tttttcaagt attgcttata ttccatcaag
     1321 ccagaatcag aaaaacggac aatatcagat attgtatcaa atttttgagg gccatttaga
     1381 taattttcat aaccagcttc aataatgggt ttcatcacga acaaagccat aatcaatgca
     1441 ataccattga gagtcatatt tgatggaacc tgctgcaaac caagtgcatt tcgtaccatt
     1501 acaaaaacaa tagaaaactt tatataacag gtaccagcag caactaaaaa tggtaatagc
     1561 gtaaaaaaag aaagtgtagc gatgagggac atgtcactca gcatgagatt actcctttac
     1621 catccaagaa ctaatctcga taccataacc atcctcaata gaaacaagtt ccccatgccc
     1681 aaccaaagaa ccatttactt taatatttac atgtttaact a
//
LOCUS       DAZSNO010000360         1687 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZSNO010000360 DAZSNO010000000
VERSION     DAZSNO010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.366"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            66..1166
                     /gene="ompC"
                     /locus_tag="ACHWN2_004399"
     CDS             66..1166
                     /gene="ompC"
                     /locus_tag="ACHWN2_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR8933626.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1330..>1687
                     /locus_tag="ACHWN2_004400"
     CDS             1330..>1687
                     /locus_tag="ACHWN2_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8933627.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINT"
BASE COUNT          509 a          345 c          351 g          482 t
ORIGIN      
        1 taaatgacgc cgtttaaaat tcgtttagaa aatacgttta acgataatta aaaggattct
       61 gttaaatgaa attaaaaata gttgcggtgg ttgtaactgg tttgttagct gcgaacgtag
      121 cacacgctgc cgaagtctat aacaaggatg gtaataaact cgacctttat ggcaaggtta
      181 ccgctctacg ttattttact gatgataagc gtgacgatgg tgataaaact tatgcccgtc
      241 tcggctttaa aggagaaacg caaatcaatg atcaaatgat tggttttggt cactgggaat
      301 atgattttaa aggctataac gatgaagcca acggctcgcg cggcaacaag acccgtctgg
      361 cctatgctgg tttaaaaatt agtgaatttg gctctctgga ctatggtcgt aactacggtg
      421 tcggctacga cattggttca tggaccgata tgttgccaga atttggtggc gatacctgga
      481 gtcagaaaga tgtcttcatg acataccgta ccaccggtct agcaacctat cgcaactacg
      541 atttctttgg cttaattgaa gggctgaact ttgccgcgca atatcaaggc aaaaataaac
      601 gtactgacaa cagtcatctt tatggtgctg acgacacgcg tgccaacggt gacggtttcg
      661 gtatctcctc aacttatgtt tatgatggct ttggtatcgg tgcggtatat accaaatccg
      721 atcggacaaa tgcgcaggaa agagccgcag ctaatcctct caatgcctcc ggtaagaatg
      781 cagaactatg ggccacaggt ataaagtatg atgccaacaa catctacttt gcagctaatt
      841 acgatgaaac attaaacatg accacctatg gggatggtta tatctctaac aaagcacaaa
      901 gttttgaagt ggtggcgcaa tatcaattcg acttcggctt gcgcccatca ctcgcttacc
      961 tgaaatcgaa aggcagagat ctgggccgct acgccgacca ggacatgatt gagtatatcg
     1021 acgttggtgc gacgtatttc ttcaacaaaa atatgtcgac ctatgttgat tataaaatca
     1081 acctgattga tgaaagcgac tttacccgtg ccgtagatat tcgcaccgat aacatcgtcg
     1141 ctacgggcat tacctatcag ttctaacgtc ctctcctgac cagcccttct ctgggctggt
     1201 ttttgattta agtgctgctg tatgactcct taagtaaaaa tgagcaattc ttgatatttc
     1261 tggtcattca cctcaattaa tattattcca tatcctccgg ggacatctta ctataatatt
     1321 tattgatgag tgattatgac caatattaat acagcttgtg taaaaaataa tgccagttat
     1381 caacttaata acgcattacc taacaaagaa accatctcca gcaatttttg tgaacgacta
     1441 gaacaatggg gtaataaatc ggttaataat ggtgaagaaa gagcaattgc cgtggagcga
     1501 attaaagaag cttacaattc gaacatggca tctcttgatt tgtcatatct tgatttgagt
     1561 gaattaccgc ctatcccctc gacagttaat acgttaaatc tggaaaacaa ttgtctaact
     1621 tgtcttgact tagctgacaa tgccagtctc gttaatatca acctcagcgt taataaaatt
     1681 aacacga
//
LOCUS       DAZSNO010000361         1654 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZSNO010000361 DAZSNO010000000
VERSION     DAZSNO010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.367"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..151)
                     /locus_tag="ACHWN2_004401"
                     /pseudo
     CDS             complement(1..151)
                     /locus_tag="ACHWN2_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(81..1517)
                     /locus_tag="ACHWN2_004402"
     CDS             complement(81..1517)
                     /locus_tag="ACHWN2_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR8933628.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMAG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKTANHLSETAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGI
                     NGPIYHTRYCYWPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIGGLIIAAYQG
                     EADGDKVIRLVRGQSYRGSRLGPVGISVPGTPTGTYIVSPQFFITGCSEHSLPGSYCA
                     LSGVPDAHVSGAMPGLFIRTSRGMHRGN"
     gene            complement(1541..>1654)
                     /locus_tag="ACHWN2_004403"
     CDS             complement(1541..>1654)
                     /locus_tag="ACHWN2_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR8933629.1"
                     /translation="KACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
BASE COUNT          349 a          464 c          412 g          429 t
ORIGIN      
        1 actgcctggt ccctgtcgtc aaaaaaaaaa cgtcacagtc tactgcgcca ctttcggtat
       61 aacggggatt aatgattttt ttaatttcca cggtgcattc ctctcgatgt gcgaataaaa
      121 agcccgggca ttgcgccaga gacatgtgca tccggcaccc cggacagggc gcaatatgac
      181 cccggtaatg aatgctctga acatcccgta atgaaaaatt gtggggatac tatatacgtt
      241 ccggtgggag taccgggcac tgaaatcccc accggtccca gtcgtgagcc tctgtatgac
      301 tgccccctga caagtctgat gactttatca ccgtcagctt ctccctggta cgcagcaata
      361 atcagcccgc caatgtcagg gtctccccat ctgttgcgga cagagctcgc cacgattctg
      421 taaataatat cttctgtggt tatatttatt ttcacccagc cagtcagtct ggatatgggc
      481 cagtagcagt agcgggtgtg ataaatgggg ccgttaatgc cgtaaaaagt aagggatttg
      541 gctctgtacc gcggttctgt tgtctcaggg cgtgcatcag tccaccggat gctgagcacc
      601 ccttcaaacc gtgtgtcggg tatgatgatg ccgtaggggc cagcaacgga atattcacct
      661 ggcagcgcat tccttaccca ggccaggaaa tcactcttag tgtcaaaacg gataacatct
      721 tcaggcagaa aagcacaccc aaagccgaat gcgccgggta tcgccagacg gccttttgtc
      781 cggtcgtaaa tgtcgctctg tgcttccatc gtggccgcac ttttcagccc cagattatcc
      841 cgggacttct gttgtgcctt ttcgcctgct gctgcggttt cagacagatg gttagccgtt
      901 ttcagagtgc cggtcagcgc agcatcaatg tcatttttgg cctgctctgc tgcgcgggca
      961 taacctgcgg catcctgtgc gctctgtccg gctgcggctg catttgctcc tgtgcttgtc
     1021 acatcttccg cagtggcctt tctgtcctca gctgtgacgg ttgcatcctg tcgggctttc
     1081 tccgcaaaac gttgtgcatc atcacgggcc gttgcggctg ccgccacatc ctgcgccgtc
     1141 tgctgtgcgt ttccggctgc ggccccggcg ctctgctgcg cctgcgccac catttcctca
     1201 aagcgtttga cgacatccgg tttcaggtcg ccctcatcag gagcaatcag aaagtcattc
     1261 agcgtgccgg gctttgagtc ctcatataca gcaatgtcgc caacgttgta ctcgttgcgc
     1321 cagtcctgtt tcagatacac accatatttt ccagtccgcg catggaaaca gtattcacca
     1381 tcgtttcctg ccatcacatc agcaacagtg ttcatcacca cttccggggt gtttacccgg
     1441 gatttcagaa taatatggta tccggacatg gggatacctg cgccatcagt cagcgcacct
     1501 gatatcacta cagacattgt ttttctcgcg ataaattaaa tcaggaagaa ccttccggag
     1561 aggcgggcca ttcaatggcg ttgtatgagg atttatcagt gatggtgctg aaatccatcg
     1621 cctgcagcga tttcgcgtaa atacgacagg cttt
//
LOCUS       DAZSNO010000362         1647 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZSNO010000362 DAZSNO010000000
VERSION     DAZSNO010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.368"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            133..562
                     /locus_tag="ACHWN2_004404"
                     /pseudo
     CDS             133..562
                     /locus_tag="ACHWN2_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            749..986
                     /locus_tag="ACHWN2_004405"
                     /pseudo
     CDS             749..986
                     /locus_tag="ACHWN2_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1100..1358
                     /locus_tag="ACHWN2_004406"
                     /pseudo
     CDS             1100..1358
                     /locus_tag="ACHWN2_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1407..>1647
                     /locus_tag="ACHWN2_004407"
     CDS             1407..>1647
                     /locus_tag="ACHWN2_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933630.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQ"
BASE COUNT          513 a          304 c          438 g          392 t
ORIGIN      
        1 tcctgccggt atcaacgccg acggcatgta ttgccctgaa tgcggtacgg ttcactggcc
       61 ggatggcgtt attccgcctt tctgatgctt ccccgttttg ccgatatttt tcagcaggga
      121 aaccgatggc ttaactggct ggagaagcag ccggtgcaga tgagccgcct ggaacattat
      181 gccggtcagg atgaaattgg cctgcgttac aacagccacc ggacaaaacg agaagaaaac
      241 cttgtgatga gtggcgacga gtttatggaa aggttctcct ggcatgtggc ggataagggg
      301 ttccggatgg tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg aagatgtggt
      361 gtacgccata acggagacag tgagaaaaac ggcgaggcaa atcacaagga gagggatgta
      421 tcagcagtgt ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt
      481 tacggggctg aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc
      541 tcgaatacgg tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa
      601 cggagcaaaa aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct
      661 ataccgatta cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt
      721 aatcttttgg ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat
      781 actgacgtca aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc
      841 aacagcgatc ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa
      901 aagagaaacg taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa
      961 tggtggaaaa cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc
     1021 atcccctaaa cggtcaaaat gcttctgccg tgaaggaggg agaatgccat ctcatcactt
     1081 atatctggcg aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa
     1141 tggaatggca gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga
     1201 acctgacgtg ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat
     1261 ggcatcctca tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca
     1321 gtaatgggga aaatgatagc tggtttctga aaatatagag gaaaatgtga tatatatatc
     1381 ctgttaatat ttaaggagaa tataaaatga aagtctcgtt taagtcttta ggctatattt
     1441 ttcatgatat atataataaa aaacacacta tcgatgagtt caacgatgtg gtaagaaaag
     1501 cagttttatc aggtaaaatt aacgagctga atgcatgtca taaagtggca atttttcttg
     1561 ccgaaaaaga caatgagata acaaagaaag acaaagccaa gataatagat acattaactg
     1621 aaaactattc cattgaattt caacaat
//
LOCUS       DAZSNO010000363         1620 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZSNO010000363 DAZSNO010000000
VERSION     DAZSNO010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.369"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(5..508)
                     /locus_tag="ACHWN2_004408"
     CDS             complement(5..508)
                     /locus_tag="ACHWN2_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR8933631.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(553..1516)
                     /locus_tag="ACHWN2_004409"
                     /pseudo
     CDS             complement(553..1516)
                     /locus_tag="ACHWN2_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT          451 a          354 c          333 g          482 t
ORIGIN      
        1 tccattaact ggcctcctgc gcaggttgtt tcagttgttg aatgctttga ttaataaaac
       61 acaaggcata aaacatcatt accactccac ttaatggcag aatgatatat acgtatccca
      121 tcggtatttg catagcggca gatacctgtg atgttgccag tgttttatca attaatttca
      181 ggccaccagt cacaatgacc gaaccggcaa aacctaaaat gagaacgtta atgactatac
      241 taagaagtaa ttgtttgcgc ttattcaggg cgagagcaaa taaatcaata gagagatgtc
      301 gctgcgcgcc gacggtatat gcagcaccta ataatcctac ccagatcatc aaaaagcgtg
      361 ccagctcatc ggttaatgta cttggctggt taagtacgta gcggctaaat acttgccaga
      421 ctacgcatac aaccagagca agcattacgg cgacagaaaa cgtggcaatg acgcggtcta
      481 ccgcaagttt caggcggtta aatatcattg taagcatcct ggaaaatcag ggccagaggc
      541 tggcccggaa tattattgag cagcgttgtc gaatttctct aacaaagcag cttgttttgg
      601 atcttttttg aagtcgtcaa acagaggttg cacggcttta cggaatggcg ctttatccac
      661 ttttacaatt tcacccccca tagctttcgc ctgagcacgt gtgtcagcgt caattttttc
      721 ccacaatttt tgttgataag cttcggattt cttcgccgct gtttcgagga tttgttgctg
      781 ttctggggtt aatttattcc atgttttggt ggagataacc aaaaagtcag gaattgaggc
      841 gtgttcatct tcagaaaaaa ctttggcaat ttcaatatgt cgagtctgca tccaggaagg
      901 cacgttattt tctgcgccat ctactacacc ttgctgcatc gcagtataaa cttcgccaaa
      961 tgaaattgga gtgggtgaac cacccatcag ttcaatcatt ttgatggtgg tcggacttgg
     1021 ctgaacacga attttcagtc ctttaagatc ttctggcttt gtaatcggtt ttttcgcata
     1081 gaaactgcga gtacccgcta cataagctga taaagcaaaa aagcctttgt cttttgttga
     1141 gtccataatt tctttaccaa cttcaccaaa aacaacttaa taagaacggc aaattataga
     1201 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
     1261 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1321 tacggatcac catattacct tttgacagct gttttacctc tttcgccagc tcttcaaaac
     1381 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgcc actttttctg
     1441 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1501 atgacctcat tttcataaaa aaactccctg ttgaaggatg ggaattggtt gatatctaat
     1561 tgtgcttatc agtgatttgt gcgacaaatt ggttaacctg tctgacaact tgtctatgtt
//
LOCUS       DAZSNO010000364         1601 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZSNO010000364 DAZSNO010000000
VERSION     DAZSNO010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.370"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(333..479)
                     /locus_tag="ACHWN2_004410"
                     /pseudo
     CDS             complement(333..479)
                     /locus_tag="ACHWN2_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            533..715
                     /locus_tag="ACHWN2_004411"
     CDS             533..715
                     /locus_tag="ACHWN2_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR8933632.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(816..1145)
                     /locus_tag="ACHWN2_004412"
     CDS             complement(816..1145)
                     /locus_tag="ACHWN2_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR8933633.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1317..>1601)
                     /locus_tag="ACHWN2_004413"
     CDS             complement(1317..>1601)
                     /locus_tag="ACHWN2_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933634.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          435 a          368 c          377 g          421 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      361 ggcaggagtt cgtcaacacg gttggagggc cattcaggca gtatgctcag aatatggcgc
      421 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc ggcaacaagc
      481 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
      541 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
      601 gaaaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
      661 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
      721 attggttctc agatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag
      781 ggttgccggg caaccctcat tgaataaaac gggaattact tcgcttcgcc gtttttcatt
      841 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
      901 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
      961 tggattgctt caacgctcat ccctggcttg atgtaatcgc tcaggttgtc cagcaacagg
     1021 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     1081 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     1141 tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt
     1201 agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt ttcactatca
     1261 taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt caggaattat
     1321 ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc cgtttccatg
     1381 ttcagccaca cataaccata gattggtgtt tccacgactt tcaggtcatg gtggttatcg
     1441 agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc aaaaaccggc
     1501 tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatgtgc
     1561 tgggtatcac aaagtttttg cgcgttcaat aacacgaaat g
//
LOCUS       DAZSNO010000365         1581 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZSNO010000365 DAZSNO010000000
VERSION     DAZSNO010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.371"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHWN2_004414"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHWN2_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFR8933635.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="ACHWN2_004415"
     CDS             complement(1269..>1581)
                     /locus_tag="ACHWN2_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFR8933636.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DAZSNO010000366         1561 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZSNO010000366 DAZSNO010000000
VERSION     DAZSNO010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.372"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            400..1149
                     /gene="phoC"
                     /locus_tag="ACHWN2_004416"
     CDS             400..1149
                     /gene="phoC"
                     /locus_tag="ACHWN2_004416"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HFR8933637.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1204..1308)
                     /locus_tag="ACHWN2_004417"
                     /pseudo
     CDS             complement(1204..1308)
                     /locus_tag="ACHWN2_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1319..>1561)
                     /locus_tag="ACHWN2_004418"
     CDS             complement(1319..>1561)
                     /locus_tag="ACHWN2_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8933638.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          427 a          332 c          315 g          487 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagaccta
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc gccaggtaat
     1381 ttctcgccgt tttgtcgtaa cgcgtggcga tgcggcgata ttctttcagc cgcccaaagc
     1441 accgttcaac gacattacga ttgcagtacg catcacgatc aagctgtgca cgtccatccg
     1501 atgccatttt ctcatttgac tttcggggga ttactgcctt tataccgtta tttttcagct
     1561 c
//
LOCUS       DAZSNO010000367         1543 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZSNO010000367 DAZSNO010000000
VERSION     DAZSNO010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.373"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            261..389
                     /locus_tag="ACHWN2_004419"
                     /pseudo
     CDS             261..389
                     /locus_tag="ACHWN2_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            579..983
                     /locus_tag="ACHWN2_004420"
     CDS             579..983
                     /locus_tag="ACHWN2_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933639.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            1046..1543
                     /locus_tag="ACHWN2_004421"
                     /pseudo
     CDS             1046..1543
                     /locus_tag="ACHWN2_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          436 a          369 c          404 g          334 t
ORIGIN      
        1 atcaatgatg tttatgtcag aaagtaataa tcctctccca caaagtgaat taaatgaata
       61 tataatgcgt tcgaagtata tgtattgagc tgtcatactg agaaacaaac cttagggcag
      121 cacaattaaa gccagaaaca actatgcaag aaagttacta aataagtgct cggaacagta
      181 aaacactcag gggggactca gtccggatta tgtaatcaga taaattgcca aacatcacaa
      241 atcaccagcc ggactgagtg atgccgagca ttgcaccaat accccgtgat gaacgatgcc
      301 tgatgcagaa agctccctat aaaacaaaga acaaaaacta cgaccggcga ttcaccgtca
      361 tactgatgct gcatcgtggt gacactgtct gtcactttca tgttgactga acaactctgc
      421 tgcgaagaag gtgtaccgcc ggcgggattt gattgtccgg cgtctagctt ccacttccct
      481 gatggaactg aaagttgtta tggccggtca gcttgccctg tccgcaggac atgaggtgct
      541 gtgacggaca gcaccagtaa cggaaagtga caatgaaaat gaatactgcg ctgatagttg
      601 cactaatgtg tatgtggtat gcggtacccg ctgctgcaaa agagacttta ctggctatgc
      661 cacgtaattc aacagaacat tgctatgcgg agattaatgt gcacggacca tatggggtgt
      721 atttccgagt ggtcccgcat ccccccgggg gaaagagctg ggtcgaatgt aacagtgact
      781 actattactc tgataaacca ccgggagtac agatactcgg aacccgtgca gggtgtcggg
      841 tatacggaat ctgcgggacc acctcgacac tgcatgtggc tggaagaggg gttgtgtgta
      901 ttaaaaatat atgcagtcca cgtgggatga taattcacag gatacggaaa cgtcctgtag
      961 tggctgtaag cgatgagatg tgatcttacc cagcaatagt agacacgcgg ctaagtgagt
     1021 aaactctcag tcagaggtga ctcacatgac aaaaacagta tcaaccagca aaaaaacacg
     1081 taaacagcat tcgcctgaat tttgcagtga agccctgaag cttgctgaac gcatcggtgt
     1141 tgctgccgca gcccgtgaac tcagcctgta tgaatctcag ctctatgcgt ggcgcagcaa
     1201 actgcagcaa cagatgacct cgtctgagcg tgaaagtgaa ctgcccgcct gaagcggcaa
     1261 ctggcggagc aggcagagga actggctatc ctccaaaagg ccgcgacata ctttgcgcag
     1321 cggctggtat acgtggtgtc agcgtcggac aggaataagc cctcgtcagc agttccgtca
     1381 gcactgcgac agcgttgtcc tcgcggcggc ctttacccgg tcaaaacagc gttacggtgc
     1441 accacgcctg acggatgaac tgcgtgctca gggttaccac tttaacgtaa aaaccgtggc
     1501 ggcaagcctg cgccgtcagg ggctgagggc aaaggcctcc cgg
//
LOCUS       DAZSNO010000368         1512 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZSNO010000368 DAZSNO010000000
VERSION     DAZSNO010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.374"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="ACHWN2_004422"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="ACHWN2_004422"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR8933640.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT          409 a          311 c          408 g          384 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tg
//
LOCUS       DAZSNO010000369         1499 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZSNO010000369 DAZSNO010000000
VERSION     DAZSNO010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.375"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            66..992
                     /locus_tag="ACHWN2_004423"
                     /pseudo
     CDS             66..992
                     /locus_tag="ACHWN2_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
     gene            1231..1290
                     /locus_tag="ACHWN2_004424"
     CDS             1231..1290
                     /locus_tag="ACHWN2_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR8933641.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
BASE COUNT          436 a          358 c          394 g          311 t
ORIGIN      
        1 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca
       61 ttaccgaaca acaagaagcg tttatgacgc ttgagcagga gcagcaggtt aaaacccgta
      121 ctgccgaaca gaatgcacgt attgcggctt ttgaagctga acgtcgtcgt gaagcagagc
      181 agacgcgaat tctggctgaa cgacaaattc aggaaacgga gatcgagcgc gagcaggccg
      241 tccgctcaag aaaggttgag gctgaacgtg aagttcgtat taaagagatc gaacagcagc
      301 aggtcaccga aatcgccaac cagacgaaat cgatcgctat tgccgccaaa tcggaacaac
      361 agtcccaggc agaagcgcgt gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa
      421 acgtagaaac cactcgccag accgcagaag ccgatcgtgc taaacaagtt gccctaatcg
      481 ctgccgcgca ggatgcagaa accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa
      541 aagaagccgc agagatgcag gcggcggcta tcgttgagtt agccgaagct acacataaaa
      601 agggtctggc ggaagcagaa gcacaacgtg cgctgaacga tgctatcaac gtactttctg
      661 atgaacaaac cagccttaaa ttcaaactgg ccttgttgca ggcgctacct gcggtaatag
      721 aaaaatccgt tgagccgatg aaatctattg acggtatcaa gattattcag gtcgatggtc
      781 tgaatagtgg cagcgctgcg ggtgatgcaa acacgggtaa tgtggggggc ggaaacctag
      841 cggaacaagc attatcagcc gctctctctt accgcacaca ggctccgctg attgactcct
      901 tgctcaatga aattggcgtt tcaggcggtt cactggcggc attgacttca cccttaatct
      961 caacaactcc cgtcgccgaa aacgtaaaat aaattttcag ctatgaacgt ccctggcaca
     1021 aaaagccggg gattttttat atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg
     1081 caacgttttg cgtgaccgct cggggaaggc aaaactgcct ctgggaaagc gttgcgcgag
     1141 gtccggcgct catcaacaat cggggagtca gcaaagggct gaaacgggaa agcccctccc
     1201 gaggaagggg ccttacagaa ggaaagggtt atgatgaagc tcgtcatcat actgattgtg
     1261 ttgttactcg taagtttcgc agcttattaa cagccaatca gaggggggag aaatcctccc
     1321 tcacccttgt tcctttactt taggttgaaa aaaaatcagc gtcaagaagg tttcattaac
     1381 acatatccct ggcccaaaaa gccggggatt ttttatatct gcgttccgct aaaaggtgca
     1441 aatgctcagg acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa ctgcttcag
//
LOCUS       DAZSNO010000370         1485 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZSNO010000370 DAZSNO010000000
VERSION     DAZSNO010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.376"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..797)
                     /locus_tag="ACHWN2_004425"
     CDS             complement(<1..797)
                     /locus_tag="ACHWN2_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8933642.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLT"
     gene            complement(1396..>1485)
                     /locus_tag="ACHWN2_004426"
     CDS             complement(1396..>1485)
                     /locus_tag="ACHWN2_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR8933643.1"
                     /translation="RTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          394 a          238 c          359 g          494 t
ORIGIN      
        1 gttaatcttt gcagggattg cagagcatgg gatgacaatg ggttatcact aatatctatt
       61 gaacattcat tcctcagatt aagaatgctt tccgggatat gacttatctg atttatgtca
      121 aaataaccgt tccttaaatt ttccggcagg tatggaatat cggttaacat attgtcgatt
      181 gcccatatgg agtgaatgtt gtgaggtaga ggggggagtt cactaagaag attccctatt
      241 gcgctaatct cttgtaaaga aaatggtaaa ggtggtaaaa ctgccagccc attacatgat
      301 acatctaatg tttccagtag ttctggtaaa acaggaagag aacataattg attatttgag
      361 acatgaagct ccttcaaaga tatggggagt gtgggtagtg tgattagttg attgtgggac
      421 gcattcaata atttaagtcc ttgaggcaaa gcaggcagtt caataagtct gttatagctg
      481 acattaagtt gtgtaaggga cgcaggcaat ggggagatta agcttaaatt atttttactt
      541 atattaattg atttaattcc cggggggatt tcaggtaatg ttgtcaggcc taattcagac
      601 aagtccaggt tcgtctcttg gttttgtaga catgatacta gtcgctgaaa agcgatgtct
      661 cgttgttctt cttgtatgcg gttatttttc cattcagtcc aatgggttag ataattttca
      721 tatgcgcggc tagtgtcgat tgaataagtg gagaacgaat ttgaaattaa tctgtgattg
      781 ttatttgtcg ggagcataaa tatcagggtg tattctttat gttgagaggt gttggattct
      841 tttttatctg ctttgcaatg ttattggttc ccttcagcag tagacaggaa cttctggagg
      901 aggcggatat atagtttgtg taagcatatt aactccatat gttatatatt gaagaacttc
      961 tgctttacac tatcctaaac tggatcggtt taatgtaaaa aaacgatact ttttgggggg
     1021 gggcaatttt tccttaaggt atgaaaaaag gagctgaagc tatataatag ctattaatgc
     1081 cactggtatc agtgaatcat gtatataaaa aactgtgtta aggaggctgt ccattagcgg
     1141 ttttttattc ttctggaacc gcggcatctt tgcgtatctt tttgtcttct caccgtctgg
     1201 tcggtgtttt gctgagactg ctaatttcct gtctttgcta gtgctgtact ggtactgccc
     1261 aatcatgatt ggcgggggcg ggcggagttg agaccgcaga cacgtcgtat gcaagaacgt
     1321 gctgcggctg gctggtgaac ttccgatagt gcgagtattg aatgatttcc agccgttact
     1381 gattttacgt gctaattagt gaacaaacca ctcgtcagca gactcccagg tatctttcag
     1441 agtttcctga acaaaagttt tagctgaatc tttatcggcg gtgcg
//
LOCUS       DAZSNO010000371         1483 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZSNO010000371 DAZSNO010000000
VERSION     DAZSNO010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.377"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..143
                     /locus_tag="ACHWN2_004427"
     CDS             <1..143
                     /locus_tag="ACHWN2_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR8933644.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="ACHWN2_004428"
     CDS             127..486
                     /locus_tag="ACHWN2_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933645.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /gene="yfdT"
                     /locus_tag="ACHWN2_004429"
     CDS             486..791
                     /gene="yfdT"
                     /locus_tag="ACHWN2_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFR8933646.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            707..1141
                     /locus_tag="ACHWN2_004430"
     CDS             707..1141
                     /locus_tag="ACHWN2_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8933647.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            1063..>1483
                     /locus_tag="ACHWN2_004431"
     CDS             1063..>1483
                     /locus_tag="ACHWN2_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR8933648.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKA"
BASE COUNT          486 a          325 c          365 g          307 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cacgcaaaga
      901 tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg cggctcttca
      961 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     1021 tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa cggtaaaaga
     1081 tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca tgacaagcta
     1141 aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac gttcgaggaa
     1201 gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga tgacaaaagc
     1261 cggatcggat tctggcttca acatttcgca ggaatacaac taagagacat tactgaatca
     1321 aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc atgaggaaaa ctggaaactc
     1381 agggcagaat catgcagaaa aaaagggaaa cctgttccag aatacacgcc aaaaccagcg
     1441 tccgttgcaa cgaaggctac gcatctttca tttataaagg ccc
//
LOCUS       DAZSNO010000372         1447 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZSNO010000372 DAZSNO010000000
VERSION     DAZSNO010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.378"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            68..472
                     /gene="stpA"
                     /locus_tag="ACHWN2_004432"
     CDS             68..472
                     /gene="stpA"
                     /locus_tag="ACHWN2_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR8933649.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(519..1043)
                     /gene="ygaP"
                     /locus_tag="ACHWN2_004433"
     CDS             complement(519..1043)
                     /gene="ygaP"
                     /locus_tag="ACHWN2_004433"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR8933650.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1053..1352)
                     /locus_tag="ACHWN2_004434"
     CDS             complement(1053..1352)
                     /locus_tag="ACHWN2_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR8933651.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          393 a          339 c          341 g          374 t
ORIGIN      
        1 ataagtgatc gcttacacta cgcgacgaaa tacttttttg ttttggcgtt aaaaggtttt
       61 ctttattatg tccgtaatgt tacaaagttt aaataacatt cgcaccctcc gtgcgatggc
      121 tcgcgaattc tccattgacg ttcttgaaga aatgctcgaa aaattcaggg ttgtcactaa
      181 agaaagacgt gaagaagaag aacagcagca gcgtgaactg gctgagcgtc aggaaaaaat
      241 tagcacctgg ctggagctga tgaaagctga cggaattaac ccggaagagt tattgggtaa
      301 tagctctgct gctgcaccac gtgctggtaa aaaacgccag ccgcgtccgg cgaaatataa
      361 attcactgat gttaacggtg aaactaaaac ctggaccggt cagggccgta caccgaagcc
      421 aattgctcag gcgctggcag aaggtaaatc tctcgacgat ttcctgatct aattactgag
      481 gccggatgtc gctgctaggg cgcgtccggc gtcgcttctc aagctcgttg gttccacggc
      541 atcttatcta acaaccttgc cattccacaa aaaccgctga ttcctgcaaa cagtaacccg
      601 gcaccaacaa agccacttaa taagaagaaa ccgctattta cggtataacc cagtacaacg
      661 ccgtttaata ttaaaccacc cgcagcgatc tgcacctggc gcattaacgg caagggttga
      721 gatttattta ccgctactgg caatccagct cttttccagc catcaatccc atcttcgagt
      781 aaaaatattt ctgcgggggc ggcaatcgct gctaatttat cggcgttatt actggtgcgt
      841 ttacctgcct ggcagtggaa tataatttgc tcgcgacgta atttaggtgg aagacctgac
      901 tgttccagca cggataatgg agccagatct gcttcaggaa tatgttcacg aagatattca
      961 tcagcgtcac gaatatcgat taactttgcg ccgcgtgcga ttaattcttg tgcatcatgc
     1021 ggcgaaatgg ttgtcaaagc catagtgact ccttacggac aatagacatt tttcagggtg
     1081 gcgataatgg catttaccgc ctcattttta atggaatata gaatgcgttg ggcatcccgt
     1141 tggctgttga taagcccttc gtcccgcata cgagcgagat gctgtgatgt cgcagaggcg
     1201 ctcagtccgg taatgcgcgt cagctctccc gcgctggtgc cgggggaacc gctaagcatg
     1261 cacagaatca gcaaccgttt agggtggctc attgctttca ataaggccgc tgcctgttcg
     1321 gtactggcct gtaattgcgc gagttcagtc atatttattt aagtattttc taaattaagt
     1381 aaactctaaa ctaaaaatgc aacatatacc agcctcagca gcgtaaatga gagtaaaagc
     1441 gtaagct
//
LOCUS       DAZSNO010000373         1404 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZSNO010000373 DAZSNO010000000
VERSION     DAZSNO010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.379"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..167
                     /locus_tag="ACHWN2_004435"
     CDS             <1..167
                     /locus_tag="ACHWN2_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFR8933652.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="ACHWN2_004436"
     CDS             152..790
                     /locus_tag="ACHWN2_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFR8933653.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="ACHWN2_004437"
     CDS             1018..>1404
                     /locus_tag="ACHWN2_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933654.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DAZSNO010000374         1380 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZSNO010000374 DAZSNO010000000
VERSION     DAZSNO010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.380"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHWN2_004438"
                     /pseudo
     CDS             complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHWN2_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHWN2_004439"
     CDS             complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHWN2_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR8933655.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          382 a          317 c          286 g          395 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
//
LOCUS       DAZSNO010000375         1356 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZSNO010000375 DAZSNO010000000
VERSION     DAZSNO010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.381"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(3..485)
                     /locus_tag="ACHWN2_004440"
     CDS             complement(3..485)
                     /locus_tag="ACHWN2_004440"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR8933656.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="ACHWN2_004441"
     CDS             complement(476..778)
                     /locus_tag="ACHWN2_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR8933657.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="ACHWN2_004442"
     CDS             1050..1322
                     /locus_tag="ACHWN2_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933658.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          397 a          342 c          288 g          329 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggtt cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccgaccgct gaaacgcctg ttcaca
//
LOCUS       DAZSNO010000376         1352 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZSNO010000376 DAZSNO010000000
VERSION     DAZSNO010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.382"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..217
                     /locus_tag="ACHWN2_004443"
     CDS             <1..217
                     /locus_tag="ACHWN2_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933659.1"
                     /translation="AEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRET
                     EQQIYRQLTDEVLALRLPENGSQLHHS"
     gene            complement(397..1020)
                     /locus_tag="ACHWN2_004444"
     CDS             complement(397..1020)
                     /locus_tag="ACHWN2_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR8933660.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RTEQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
     gene            complement(971..1352)
                     /locus_tag="ACHWN2_004445"
                     /pseudo
     CDS             complement(971..1352)
                     /locus_tag="ACHWN2_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          299 a          396 c          338 g          319 t
ORIGIN      
        1 ggcagaagag cagaaatatg agatgctgga gaatgagtac cctcagaggg tggctgaccg
       61 gctgaaagca tcaggtctga gcggtgatgc ggatgcggag agggaagccg gtgcacaggt
      121 gatgcgtgag actgaacagc agatttaccg tcagctgact gacgaggtac tggccctgcg
      181 attgcctgaa aacggctcac aactgcacca ttcataatca catcgcataa accacagacc
      241 ggactgactc cggaaaaaca gaggcccgcc cccgggcctc cccggattca tccgtttctc
      301 tgttcagcct gaccgcacac cccggcggcc ggatgacaga ctccgcctcg gtaagcaaag
      361 cggtcttctg tgattccgcc agttgcggct tattcattac ttaacgtcaa acgcccgaat
      421 tgaagccaaa tcatccagac tgctcagctc ctctttcatc tcccgctgac ggcgataaat
      481 ctcatcgttg cgatcgacct gcgcctgcac cattgctgcc gccagttctt ccagttccgg
      541 catcgacagt ttcacctgct gattatccgc atcactccac accatatgtg tttgtgccgc
      601 gacagatttt gccagcatga ccaccgggga caggcgggcc actgacgagg cgtcagcgtt
      661 ccacgtgcgg ccgttccatt caaacgtgaa cggcttcgcc tcctgttctg tgcgccatgc
      721 ttcaatttcc tgacgtctgg cctctctggc cgcttccagc atttctggtg tcacagtgaa
      781 tggggctatc tcaccccatt tgccactttg cagttcctgc cagatttgct gacccgtcgg
      841 tgcaacatca tcagcggtgg ctgtgtaggg aactgcctgg tccctgtcgt caaaaaaaac
      901 gtcacagtct actgcgccac tttcggtata acggggatta atgatttttt taatttccac
      961 ggtgcattcc tcacgatgtg cgaataaaaa gcccgggcat tgcgccagag acatgtgcat
     1021 ccggcacccc ggacagggcg caatatgacc ccggtaatga atgctctgaa catcccgtaa
     1081 tgaaaaattg tggggatgct atatacgttc cggtgggagt accgggcact gaaatcccca
     1141 ccggtcccag tcgtgagcct ctgtatgact gccccctgac aagtctgatg actttatcac
     1201 cgtcagcttc tccctggtac gcagcaataa tcagcccgcc aatgtcaggg tctccccatc
     1261 tgttgcggac agagctcgcc acgattctgt aaataatatc ttctgtggtt atatttattt
     1321 tcacccagtc agtctggata tgggccagta gc
//
LOCUS       DAZSNO010000377         1351 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZSNO010000377 DAZSNO010000000
VERSION     DAZSNO010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.383"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..243
                     /locus_tag="ACHWN2_004446"
     CDS             <1..243
                     /locus_tag="ACHWN2_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8933661.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            358..>1351
                     /locus_tag="ACHWN2_004447"
     CDS             358..>1351
                     /locus_tag="ACHWN2_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR8933662.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRGIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVS"
BASE COUNT          373 a          338 c          361 g          279 t
ORIGIN      
        1 gagctgaaaa ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca
       61 tcggatggac gtgcacagct tgatcgtgat gcgtactgca atcgtaatgt cgttgaacgg
      121 tgctttgggc ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga
      181 aattacctgg cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat
      241 taagggacac agcctaaata gctgcgcgga atagtagatc cctttgaggg aacttagccc
      301 ggattgtgcg atctgatcaa tcgccaaatc aaaacaaatc accaaccgga ctgagcaatg
      361 ccgatcatag caccaatttc ccgtgacgaa cgacgcctga tgcagaaagc catccataaa
      421 acacacgata aaaattatgc ccgcagactg actgccatgc tgatgctgca ccggggcgac
      481 cgtgtcagcg acgttgccag aacgctctgc tgcgcccgtt cctctgttgg acgctggatt
      541 aactggttca cgcagtcggg tgttgaggga ctgaaatcat tacctgccgg gcgtgcccgt
      601 cgctggccgt ttgagcatat ctgcacactg ttacgtgagc tggtaaaaca ttctcccggc
      661 gactttggct accagcgttc acgctggagt acagaactgc tggcaataaa aatcaatgag
      721 ataaccggct gccagttaaa tgccggaacc gttcgccgct ggttgccgtc tgcggggatt
      781 gtgtggcgaa gggctgcgcc aactctgcgt atccgtgacc cgcataaaga tgaaaagatg
      841 gcagcaatcc ataaagcact ggacgaatgc agcgcagagc atccggtctt ttatgaagat
      901 gaagtggata tccatcttca tcccaaaatc ggtgcggact ggcaactgcg cggacagcaa
      961 aaacgggtgg tcacgccggg acagaatgaa aaatattatc tggccggagc gctgcacagc
     1021 gggacaggta aagtcagcta tgtgggcggc aacagcaaaa gttcggcgct gttcatcagc
     1081 ctgctgaagc ggcttaaagc gacataccgt cgtgcgaaaa ccatcacgct gatcgtggac
     1141 aactacatta tccacaaaag ccgggaaaca cagagctggc tgaaggagaa cccgaagttc
     1201 aggggcattt atcagccggt ttactcgcca tgggtgaatc atgttgaacg gctatggcag
     1261 gcacttcacg acacaataac gcgtaatcat cagtgccgct caatgtggca actgttgaaa
     1321 aaagttcgcc attttatgga aaccgtcagc c
//
LOCUS       DAZSNO010000378         1338 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZSNO010000378 DAZSNO010000000
VERSION     DAZSNO010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.384"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            431..600
                     /locus_tag="ACHWN2_004448"
                     /pseudo
     CDS             431..600
                     /locus_tag="ACHWN2_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1338
                     /locus_tag="ACHWN2_004449"
     CDS             746..>1338
                     /locus_tag="ACHWN2_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR8933663.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRG"
BASE COUNT          325 a          312 c          411 g          290 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcgg
//
LOCUS       DAZSNO010000379         1309 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZSNO010000379 DAZSNO010000000
VERSION     DAZSNO010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.385"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..603
                     /locus_tag="ACHWN2_004450"
     CDS             <1..603
                     /locus_tag="ACHWN2_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HFR8933664.1"
                     /translation="KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQF
                     PNKQRYKIVGNIPYHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHT
                     QVSIQQLLKLPAECFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQL
                     FTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          464 a          235 c          214 g          396 t
ORIGIN      
        1 aaactggcta aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac
       61 ttatcgtcag aaaaattaaa actgaatact cgtgtcactt taattcacca agatattcta
      121 cagtttcaat tccctaacaa acagaggtat aaaattgttg ggaatattcc ttaccattta
      181 agcacacaaa ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt
      241 gttgaagaag gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg
      301 cacactcaag tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa
      361 ccaagagtaa acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa
      421 tattggaagc tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg
      481 tttactaaaa atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc
      541 gttacttatg agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa
      601 taattctatg agtcgctttt gtaaatttgg aaagttacac gttactaaag ggaatgtaga
      661 taaattatta ggtatactac tgacagcttc caaggagcta aagaggtccc tagactagca
      721 agaagtacac aagaagcctt aaagattata gaaaagcttt tccatgaaat gcttaacatt
      781 atttaatgtt aacatgtggc tttgtggtaa ctaccacgag tggtaacttg taacatgaaa
      841 aaagttacaa gttacaagtg gtttatttac gaacataaag gctaggaaag cacttgataa
      901 gtatatcaag tgcttttttt ccgttcgatt ttactatcca agataccgct tgacgtacct
      961 aatattttag tgtgctaagg gctttgcttt ttctggttat agttcgttca aaaaagaccg
     1021 caagggtaaa tgcacgatca tagatcgccc ttgcagcttt ttaatctctc cactttttta
     1081 taaccaagca aaccaccaag cacactgaaa aaataattag gaactaaagc gaagtgcgta
     1141 tcttggataa atcttcactc gattttaaaa gattaagcac ccagccaaag cgcctaaaaa
     1201 aataattttt ttaaagaaag gaagtaattt aaatgactac aattttaagc gactacaata
     1261 aatctatcct aattgaatta ctaaacacca atcgccaaat tatcgtcgt
//
LOCUS       DAZSNO010000380         1291 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZSNO010000380 DAZSNO010000000
VERSION     DAZSNO010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.386"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(52..996)
                     /gene="leuO"
                     /locus_tag="ACHWN2_004451"
     CDS             complement(52..996)
                     /gene="leuO"
                     /locus_tag="ACHWN2_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR8933665.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          397 a          286 c          255 g          353 t
ORIGIN      
        1 aaaaaatgca gaataaacca gacattcatg tctggcctat tctgcattca gttagcgttt
       61 gcaaattgag actaattgtt cttccatcca ctgatggcct ttatcgcgcc cggcagcttc
      121 atgccaagag agataacagg ttctgctgtt ttgttttaac ggcagcggta atacctgtaa
      181 ttctaaggat tcagcgaact cttcagccag ccaacgcggc gcaatagcga ccaaatgcgt
      241 ttgcgacacc acgctaagta cgctcatcat tgccatgccc tgatacgcga tactggcttg
      301 cttatctacc gtgtcatacc aaggttgact aaatgacgcg aaacgatcga gcgaaaccgc
      361 cgcatgttgt tcgttataaa catcatgttt cagtaacggg cctttaattg ttggatgatt
      421 tttgctggct accagcacca tttcatcttt aaataatggc acgctggtaa attcaggacg
      481 atggaagtct tcataactaa tcacaaactc cgtttcctga taacgcagct gatgttcagt
      541 gttctgattt aatgaagact tgaacataac atgtatattt ggcgcaatct gctcaatgtg
      601 attataaatc tgcgaggtca gaatgctgtc taacgggctg caaacacaaa gatgaaatac
      661 acgttcactg ctcgcgggtt caaaacctga accaggcaat tcattttgta ctagttgcaa
      721 tgcctggcga actgaaccaa aaagttgaaa tgcgcgagca gtcggttgaa taccacggcc
      781 ataacgaaca aaaagctcgt cattaaacat caccttcagg cgtgcaacag cgttactgac
      841 cgcaggttgc gacataccca gaacatgagc ggcacgggta atgttttgct cctgcatcac
      901 ggcatcgaaa acggttaata agttgagatc gaccatgcgt agctgtggct tgcttaactc
      961 cgccgtctct gaatgatctg tttgtacctc tggcatactt aactccactg tcacacttaa
     1021 ctccctttcc cttattggaa tgcagagaaa taatcctgaa gaatatgatt tatcatgcat
     1081 ataaaataag aaaaagcaaa atgagtaaaa ttcggaaaac ataaagatac gggttctgat
     1141 aaaaacagaa tccattgccg tggaatgagt catttaccac agcataataa tccataatgt
     1201 tttgttgcga aaacaatcta atcataacta ccgcgaatac tcaatcatct acaaaatgga
     1261 ttaaatgtgc ttatttaata attaatttat a
//
LOCUS       DAZSNO010000381         1286 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZSNO010000381 DAZSNO010000000
VERSION     DAZSNO010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.387"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            22..273
                     /gene="mxiH"
                     /locus_tag="ACHWN2_004452"
     CDS             22..273
                     /gene="mxiH"
                     /locus_tag="ACHWN2_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFR8933666.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            286..579
                     /gene="mxiI"
                     /locus_tag="ACHWN2_004453"
     CDS             286..579
                     /gene="mxiI"
                     /locus_tag="ACHWN2_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR8933667.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            585..>1286
                     /gene="mxiJ"
                     /locus_tag="ACHWN2_004454"
     CDS             585..>1286
                     /gene="mxiJ"
                     /locus_tag="ACHWN2_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR8933668.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTG"
BASE COUNT          447 a          187 c          253 g          399 t
ORIGIN      
        1 ataaaaataa gtgaggataa aatgagtgtt acagtaccga atgatgattg gacattgagt
       61 tcattatctg aaacttttga tgatggaact caaacattac aaggtgaact aacattggca
      121 ctagataaat tagctaaaaa tccttcgaat ccacagttgc tggctgaata ccaaagtaaa
      181 ttatctgaat atacattata taggaacgcg caatccaata cagtgaaagt gattaaggat
      241 gttgatgctg caattattca aaacttcaga taatagggag cattcatgaa ttacatttat
      301 ccagtcaatc aggttgatat tatcaaagcc agtgattttc aatctcaaga gatatcaagt
      361 ctggaagacg tcgtgtcggc taaatatagt gatattaaga tggatacaga tattcaagta
      421 tcacaaataa tggagatggt aagcaatcca gaatcattaa acccagaatc tttggccaag
      481 ttacagacga cgctctcaaa ttattcaata ggagtatcat tagctggcac gttagcaaga
      541 aaaacagttt cggctgttga aactttatta aagtcttaat ttatatgatt aggtataaag
      601 gttttatttt attcttgttg ctgatgttga ttggatgtga gcaacgtgaa gagttaattt
      661 ctaatttatc tcaaagacag gcaaatgaaa taatatctgt gctagaacgc cataatatta
      721 ctgctagaaa agttgatgga ggtaaacagg ggatctcggt acaagtcgaa aaggggacat
      781 ttgcatcggc agttgatttg atgcgcatgt acgatttgcc aaatccggag agagttgata
      841 tctcacaaat gtttcctaca gattcattag tgtcttctcc aagagctgaa aaggcccgtt
      901 tatatagtgc tattgagcaa cggctggaac agtctttagt ttctattggt ggtgttattt
      961 cggcaaaaat acatgttagc tatgatcttg aagaaaaaaa tatatcttca aaaccgatgc
     1021 atatatcagt aatcgctata tatgactcac cgaaagagtc tgaactatta gttagtaata
     1081 ttaagcgatt tttgaaaaac accttttctg atgttaagta tgaaaatata tctgtcatat
     1141 taactccgaa agaagaatat gtttatacaa atgtacaacc tgttaaggaa gttaaatcgg
     1201 aatttttaac aaatgaagta atatatttat ttctcgggat ggctgtacta gttgtcattc
     1261 ttttggtatg ggcattcaaa acaggg
//
LOCUS       DAZSNO010000382         1278 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZSNO010000382 DAZSNO010000000
VERSION     DAZSNO010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.388"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>1278
                     /gene="gadC"
                     /locus_tag="ACHWN2_004455"
     CDS             <1..>1278
                     /gene="gadC"
                     /locus_tag="ACHWN2_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFR8933669.1"
                     /translation="KKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIFYYTFAAIMFL
                     VPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYFPTVLTFGAVS
                     VAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSKWCGIIGTIIP
                     AIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAYAGMEMNAVHV
                     NDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLLVAYDMLFEWA
                     GVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKTNRHGMGHHLM
                     FVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIYLRHSQPDTKR
                     PYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPT"
BASE COUNT          308 a          243 c          280 g          447 t
ORIGIN      
        1 taagaaaatg acagtgggga cactcgctat catgaatata actgctgtag taagtctacg
       61 atttatacct tctgaagcag aatacggctt aggtgccatt ttttactata cctttgctgc
      121 aattatgttt ttagtacctg ttgcctttgt tgcggctgag ctagctacaa catatccaca
      181 aaaagggggg gcatttcgat ggataggaga agcatttggg gctcgctggg gttttgtagg
      241 aatgcttatg acctggctac aagtcatccc ctactttcca acagtactga catttggtgc
      301 agtctcggtt gcatttattg atgttgatat gggggtggca gaatcaattg ccagtaataa
      361 atggtatatt ttatttttcg tactatttgt ttactgggga gctatttgtg ttgcattacg
      421 tggcgttggt attttctcgc aggtatctaa gtggtgcggt attataggga caattattcc
      481 agctattatc cttgttattc ttggtttctc ctatctgtgg ttcagcggta aaccagcggt
      541 gatcccatta ggttggggtg atcttttccc taatatagct aacttccaga atattgttct
      601 tgccgccagc atatttcttg cttatgcagg gatggaaatg aatgcggtcc atgttaacga
      661 tttagataat ccaactaaaa aatacccaat tgcaattacc atcgcttcta taggtactat
      721 tgcaatattc ctgctcagta ctttaggggt cgcctttatt attcctacag ataaaattag
      781 tctaactcaa agtttgttgg ttgcctacga tatgctgttc gaatgggctg gagtgccatg
      841 gcttggctct gtgatggcat ttatgctagc aattggcgta ctgggtgggg tggttacttg
      901 gattgcaggg cctaatacag gtgtattagc gatcgcgaaa gctggttatt tacctaagtt
      961 tttccaaaaa accaatcgtc atggaatggg gcaccatcta atgtttgtac aaggtataat
     1021 tgtctcagtt ctttctgtta cttttgtgat aatgccttca gttcaagcag cttttcagat
     1081 tttatcacag ttaacagtta tgctctattt agttatgtat atgctcatgt tcgctagtgc
     1141 tatctatcta cgacattctc aacctgatac aaaacgccca tatcgagccc ctgctttatt
     1201 tttctgggca acacttggat tcttagggtc ttttctggct tttgtattaa gttttattcc
     1261 gcccgagcaa atcccaac
//
LOCUS       DAZSNO010000383         1275 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZSNO010000383 DAZSNO010000000
VERSION     DAZSNO010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.389"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            34..435
                     /gene="gpFI"
                     /locus_tag="ACHWN2_004456"
     CDS             34..435
                     /gene="gpFI"
                     /locus_tag="ACHWN2_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR8933670.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="ACHWN2_004457"
     CDS             447..818
                     /locus_tag="ACHWN2_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR8933671.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="ACHWN2_004458"
     CDS             805..>1275
                     /locus_tag="ACHWN2_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR8933672.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          285 c          396 g          291 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAZSNO010000384         1269 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZSNO010000384 DAZSNO010000000
VERSION     DAZSNO010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.390"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..825)
                     /gene="ipaH4.5"
                     /locus_tag="ACHWN2_004459"
     CDS             complement(<1..825)
                     /gene="ipaH4.5"
                     /locus_tag="ACHWN2_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR8933673.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDY"
BASE COUNT          358 a          227 c          281 g          403 t
ORIGIN      
        1 gtagtccggc gaagaggtta atctttgcag ggattgcaga acgtgagtcg acaatggatt
       61 ccctgaaatg ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt
      121 tagtaggttg ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg ataatctgcg
      181 tagttgattt tcacctacct tcaaatattt taagctctga ggaaattctg gtagttcctg
      241 tagtctgttg ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt
      301 aaggcgatta ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc
      361 cagactatta aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga
      421 caaatttcga ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat
      481 attcaagttg ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga
      541 gattaactca ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga
      601 acgtagtttc agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa
      661 taatcgaacc gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca
      721 gatgcggtgg taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa
      781 gccacaaagg gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac
      841 tattcatatc ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaacaatcct
      901 agagctttat cggcgttttc attccggctg gaggaatatg ttcctgtctt tccagtaacc
      961 gtcgattgtt atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg
     1021 gattgatctt tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc
     1081 gatattccca tagcaaacga tctggttggt tatcccctca aaaaagtcag ttgcacgccg
     1141 taaatccctg ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt
     1201 cacagttttt ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat
     1261 ggtgcagtc
//
LOCUS       DAZSNO010000385         1259 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZSNO010000385 DAZSNO010000000
VERSION     DAZSNO010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.391"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1183)
                     /locus_tag="ACHWN2_004460"
     CDS             complement(<1..1183)
                     /locus_tag="ACHWN2_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8933674.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGSEQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          413 a          290 c          245 g          311 t
ORIGIN      
        1 atgcggctcc ggcagcttct ttaggaacaa gctccattga ataaagacca ataaacatca
       61 ccggaccata cactgccagc cccaacagaa taaggattgt atagtttagc cagagatatt
      121 cattgcctga tggcacgtac cagtacgcga ttaataaaat aatggtaagt gccagaaata
      181 aagtatttgt taatggtttt gagtttctga acagtctgac agacaggtat ccggcaagaa
      241 gcataccagc cacaccaccg atttcaaaaa cagaaccccc ccccattgat gccagttttc
      301 ccatatcctt accaccgaca tccagactgt cacttaaaaa cttaggagcc caggaaacaa
      361 taccgtatct cagaatataa acaaacgaac catagataca gcagtaaatg agtttattat
      421 ttcggatgat atgttttctg atgatatcag gaatggagag tctcggacct tgttcactgc
      481 ctttagccgg gtgtgaaacg acgttacctt tccattcttc aatgggagga agccccactg
      541 atacaggtct gtccacctga ataaagtaaa tcagcacact cattgccata cacagtactc
      601 ctgggaagat aaatgcggcc cgggcatgat ccagagcact gtcgccgaaa aaaccaaaac
      661 tgaaaccaat cagaacgggg gccagagctc ccccgacatt gtgcgataaa ttccacagtg
      721 tggcccaggt gatcctttcc cgatgttcat accagaacac cagtgttttg gcaccaggag
      781 gatatcccat accttggatc cagccgttaa gtaaatacat tgttgaaaat aacgtaacgg
      841 atgtgatgat cccgggaata tatcctatca gaaaatttac cagtccggac aggaataaac
      901 caagagacaa aaatactttt gaattacttc tgtcagaaat aagtccggaa acaaactttg
      961 atataccata ggataaaaaa ccggcacagg agataatgcc gatctcggtt gcagacatgt
     1021 gcagcacacc ggataacaca ctggtggtgt ttcggacaaa ataatacccc atatatccga
     1081 ggaaaatgct gataaagacc ctgtatcgca ggacgggata taatcgttca atctcttcag
     1141 agggtaatct ttcaatttcc ttaggttctg tgaataatga catatttaat ccttctgtaa
     1201 tactgataac caattgtcgc aaacctgttt acttatcatt atataatcac tcaaagtat
//
LOCUS       DAZSNO010000386         1242 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZSNO010000386 DAZSNO010000000
VERSION     DAZSNO010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.392"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            108..>1242
                     /gene="pdeF"
                     /locus_tag="ACHWN2_004461"
     CDS             108..>1242
                     /gene="pdeF"
                     /locus_tag="ACHWN2_004461"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR8933675.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT          278 a          277 c          280 g          407 t
ORIGIN      
        1 ttagaatcaa tgctctctat agtgatgaat aatcatcatt cgaagtcagg tgggatgcct
       61 gtctgaatac acctccttca ggatgtgggg gattctgctg agcatctatg aaactgaatg
      121 caacttatat aaaaatacgt gataaatggt gggggcttcc gctgttcctg ccttctttaa
      181 tcttgcccat tttcgcccac attaatactt tcgcgcatat ttcttccggt gaggtttttc
      241 tcttttatct gcctttggca ctgatgatca gcatgatgat gtttttcagc tgggcggcat
      301 tgccagggat caccttaggg atttttgtcc gcaaatatgc agagctgggt ttttacgaaa
      361 cgctctcatt aacggctaat tttattatca ttatcattct ctgttggggc ggttacaggg
      421 tctttactcc ccggcgtaac aacgtttcac atggtgatac ccgtttaatt tcccagcgta
      481 tattctggca gatagtgttt cctgcaacgc tgtttctgat acttttccag tttgctgcat
      541 ttgtaggatt actggcgagc agagaaaatc tggtcggtgt catgcctttt aacctcggga
      601 ctttaatcaa ttatcaggcc ttactggtgg gtaatctgat cggtgtcccg ctgtgctact
      661 tcatcattcg ggtagtgcga aatccatttt atttacgtag ctattattcg caattaaaac
      721 agcaggttga tgccaaagtc accaaaaaag agttcgcgct ctggctactg gcattaggtg
      781 ctttattgtt gctgttatgc atgccgttaa atgaaaaaag cacaattttt agcaccaatt
      841 ataccttgtc attattgctg cccctgatga tgtggggagc aatgcgctat ggttataagc
      901 tgatttcgct gctctgggcg gtcgtgttga tgatcagcat ccacagctat caaaattaca
      961 ttcccattta tcctggctat accacgcagc tgaccataac ctcctccagt tatctggtat
     1021 tctcttttat tgtcaattat atggctgtac tggcaactcg tcagcgagcg gtagtcagac
     1081 gcattcagcg gcttgcgtat gtggacccgg tggttcatct gccaaatgtt cgcgccctga
     1141 atcgcgcgtt gcgtgatgcc ccctggtctg cgctttgtta tttacgcatc cctggcatgg
     1201 aaatgctggt taagaactat ggcatcatgc tgcggattca at
//
LOCUS       DAZSNO010000387         1241 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZSNO010000387 DAZSNO010000000
VERSION     DAZSNO010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.393"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(275..>1241)
                     /locus_tag="ACHWN2_004462"
     CDS             complement(275..>1241)
                     /locus_tag="ACHWN2_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR8933676.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          370 a          273 c          267 g          331 t
ORIGIN      
        1 agtgtattta aaaatgtgtt gtattagcga ctgaaatctg ccttttaaaa aggaggaggg
       61 tactgagaca aagagtcatt gcttctccga taaaatgtca gagaagcatg attacgtggt
      121 tattgtctgt tgatgacttg ttgaccgagc cgtcatcatt ctcaatgcat tagttaaatc
      181 atttgtgatt tttaaggatt atcctgtcta tgattttcta cttagaagga tatggcacta
      241 actatgcatc ttatagttta ctgctggcat ctatttaaaa tagcaattga gtcaaatagc
      301 ccatcaagat agccatacct aatataaccg tcaggaatgc agctatcatc ggcattctga
      361 acattgattt aagtaatatc acttccgtca ggcttgcgcc ggcactgccg atgattaatg
      421 ccattaatgc tcccagaccc attccttttg tcatcagaac agatgccaga gggataaccg
      481 cctcagcccg gatatacagc ggaataccga caacggcgct taatgggatg gcgaagggat
      541 tatctgctcc tgcatgagcg gcaatccacg ctgagggaat aaagccataa ataaaagatc
      601 ctattaatac tcctaacaaa agatatggca gtacatcttt aaactgttgc aacgcatctt
      661 tggtggctag ttttatggga ttaatactta ctacagcttt ggtactgcaa caggtaacta
      721 cggggttctc aatggcttta gccgctggac tacaacaact cacttttatg ggcgtatatg
      781 tcgttcccgg cgaagttttt gctggagcac aacaatttgc tgatgagctt ttactggcaa
      841 tgatatgacg ttcaaacccc agagaatcca ggataatact ggcaagaacg gagacgccgg
      901 ctgcgataat cgcgtacaac aaggtgactt tccaaccaaa ggtcatccac attagtccga
      961 cgataatggg attaagtaat ggtgaaacaa ataaaaaagt gagagtcgga ccaaacccgg
     1021 ctttcgctga caacaatcca cgtaacatgg gtattgtcga gcaactacag aacggggtaa
     1081 cggctcccaa cagagcagcc aggagatagc ctctcccttt tctggccccc atcatctgct
     1141 ggattttatg gtctggcacc ttttgtctta tcaggctgac accggcacta atcactataa
     1201 acagcaacga aagctcgacg gcgagaaaca caaacatctc t
//
LOCUS       DAZSNO010000388         1236 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZSNO010000388 DAZSNO010000000
VERSION     DAZSNO010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.394"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            2..>1236
                     /locus_tag="ACHWN2_004463"
     CDS             2..>1236
                     /locus_tag="ACHWN2_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR8933677.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKN"
BASE COUNT          437 a          179 c          227 g          393 t
ORIGIN      
        1 tatgataaac aatatatctg atcaagcatc atcatttcca ggcacacaac taaatcaaag
       61 tgataatttc ctggattcac tgagggaatt tttcgcgata ttaaatccca gtcgtaaggg
      121 tgagttgtct acatgggata ccatctactt gcatctgata ttagcaataa atgctgacag
      181 tgatctcata aaaaatgatg tgttgctcgc ggaaaatatt ccatcagcga attaccaatt
      241 taatacattt tttagcaata ctttcgagat tgatgttaaa aaatatctgg gtaaatcaga
      301 agataacgaa gttgaaataa aagcgggtaa tgaaagaatc tctattggaa tacgtaatgt
      361 atcgaatgga aaacttgaaa gacaacagtt tcttttccca ctggactatg agaataaatt
      421 acaagaacaa ttagataagt attttacgat tgaatcgcac ccattattat acaggtatac
      481 cattggtagt aagattgcga atgtgatttt tgaaaagtta tacagtcgta ttgactttaa
      541 caaagaacaa tatatatcct ttataaagga cgcatttatt catttttatg attatagcag
      601 gcgatatgct attagtgaga atatcgataa agacgctgta acaaacaata tcgcattgat
      661 gagcactttt tatgacagtg ataatacgtc aggtgaggtt ttaaataatg attttacaga
      721 agaagagtca tttgaaactg cgttagatgt agagcatgca atagtgcttg gatttgctga
      781 tgataatttt gaaacaaaac ctgttcatta tcaggattta ttaacacgat ttagtgcctt
      841 tcaagatacc gtgtttaatc tgtttcctga aatgcactct tcgcattacc acgatatttg
      901 tagcgtaagt gtggatatga caaagggaac gcaatgcatg attcacttga tggtgaatga
      961 agaagttttc atgtctctgc ctgttccagt tgccacgatg gtacgtgaag atgcaagtaa
     1021 tttagtcaat cttaaaacac tactcaatga tggttgtttc attaaatata gtcattttaa
     1081 tgacgtggca ttaataaagc aaaacataag taatctctat ttatcacaca ctgttgtgaa
     1141 tgaaagcata ttgaaaaaat gttgttttga aaacggcagt ttaggtgatg ttaaaataac
     1201 aaatagcaat gtaataaaca gtgcattcaa aaatat
//
LOCUS       DAZSNO010000389         1233 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZSNO010000389 DAZSNO010000000
VERSION     DAZSNO010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.395"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..730
                     /locus_tag="ACHWN2_004464"
     CDS             <1..730
                     /locus_tag="ACHWN2_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFR8933678.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="ACHWN2_004465"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="ACHWN2_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          325 a          303 c          272 g          333 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
     1141 tctgaatcgc cacgggttta acagacacct cagagtcatt taagatggct taaagagagg
     1201 tgcccatgag cggtaagcgt tatcccgaag agt
//
LOCUS       DAZSNO010000390         1232 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZSNO010000390 DAZSNO010000000
VERSION     DAZSNO010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.396"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACHWN2_004466"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACHWN2_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR8933679.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          365 a          206 c          294 g          367 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gt
//
LOCUS       DAZSNO010000391         1216 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZSNO010000391 DAZSNO010000000
VERSION     DAZSNO010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.397"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..254)
                     /locus_tag="ACHWN2_004467"
     CDS             complement(<1..254)
                     /locus_tag="ACHWN2_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HFR8933680.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEML"
     gene            complement(241..873)
                     /gene="mxiE"
                     /locus_tag="ACHWN2_004468"
     CDS             complement(241..873)
                     /gene="mxiE"
                     /locus_tag="ACHWN2_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFR8933681.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            complement(1003..>1216)
                     /gene="mxiM"
                     /locus_tag="ACHWN2_004469"
     CDS             complement(1003..>1216)
                     /gene="mxiM"
                     /locus_tag="ACHWN2_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HFR8933682.1"
                     /translation="KAVYSFNAGKFMGNFNVKEVDGCFMDAQKIAIDKLFSMLKDGVV
                     LKGNKINDTILIEKDGEVKLKLIRGI"
BASE COUNT          405 a          239 c          153 g          419 t
ORIGIN      
        1 tcaagcattt cttctggatt agataaatca aactccccag atattctctt ttttgcagct
       61 tgtttgctaa caacaatagg ataatttaat aacgcagaaa aacgttcaaa aaaagatcct
      121 accgtatcac tttgagcaac atatttcgct atgtcatttt gttctaataa atgagagtca
      181 atattattag cattaactat caatggtaac aatacaatca agagagtcaa agatttaata
      241 ttaaattttt tcatttattt ttttcactaa aaaagtaata ttcgataatt ctcttgcaga
      301 aaagcctagt cgtgttttta tctcatttga aaaatgggat gttgatgcat acccattatt
      361 catggcagct gaagtgattg tttgattgtg caaaaataca tctaataaac catttactaa
      421 ccgccaagtg ttcagttgtt ctttgacttt tgcccctaaa gcttttctac ataaagatct
      481 aaaataagcc tccgacacac cataatgctc tgtcagactc tttattctta cctccttttc
      541 tttctcaata gttctcagaa gataaagaac caaatgatat gactcagtca ttcgtatcat
      601 agccagtgca ttacttacat tctggtcaga tgaaaagatg aatcttaaca ttaacttatc
      661 aagcatcgat tcggtattac aactatcccg aagatcattc tcatgataga aatgaataca
      721 tttttcagca tggctatgct ctactagagc accactgaaa tagtcccaaa aagcaagaaa
      781 ttttgcacaa ctattaatgt taaagcgata atgatagttc acattatcac tgaattttat
      841 attttgattt cggacaaaaa aaaacccttc catgctggag ccagctcaat tatgttacgt
      901 tctttatctt ttacaagctc aacgttaact ggttcatgtc cactagagta aatgtaaaac
      961 atattactgg tatttagacc tttatattta ctcacaatac aattatatcc ctcgaattaa
     1021 ttttaattta acttccccat ccttctctat aaggatggta tcatttatct tattaccttt
     1081 taaaacaacc ccgtctttca gcatagaaaa tagtttatct atagctatct tttgtgcatc
     1141 catgaagcac ccatctactt ccttaacatt aaagttgccc atgaatttac ctgcattaaa
     1201 actataaacc gcctta
//
LOCUS       DAZSNO010000392         1211 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZSNO010000392 DAZSNO010000000
VERSION     DAZSNO010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.398"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..1131)
                     /locus_tag="ACHWN2_004470"
     CDS             complement(<1..1131)
                     /locus_tag="ACHWN2_004470"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR8933683.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPA"
BASE COUNT          286 a          319 c          319 g          287 t
ORIGIN      
        1 cgccgggatg ccttcgtccg ggcgacccgt acagatgaac tcatacggcc cctgtttggc
       61 gatttcatcc gccagataag cggcaacctg gtaagaggcg ttctgtactt tggtatagcc
      121 ttcacgaccg aggcgcagga attcatagta ctgtgcaatt acctgacccg ccgggcggga
      181 gaagttgatg gcaaaagtac caatttgacc acccaggtag tcaacgttga acaccagttc
      241 ctgcggcagc gcttcttcgt cacgccagat aacccagccg cagcccagcg gagccagacc
      301 gaatttatgg cctgaagcac tgatcgattt cacacgcggc aggcggaagt cccagacgat
      361 atccggggcg acgaacggtg ccaggaagcc accgctggca gcgtcgatgt gcatgtcgat
      421 gtcgataccg gtgtcggcct ggaatttatc cagcgcatcg tgcagcggtt gtgggaactc
      481 atagttaccg gtgtaggtca cgccgaaagt cggcaccacg ccgatggtgt tttcgtcaca
      541 ggcttcaatc atgcgtttcg ggtccataaa caactgaccg gggcgcatag ggatctcacg
      601 cagctccaca tcccagtagc gggcgaattt atgccagcag atttgtaccg gaccgcacac
      661 taggtttggt ttatccgttg gtttgcctgc agcttccata cgcttgcgcc aacgccattt
      721 catcgccatc ccgccgagca tacaggcctc ggaagaacca atggtgttgg tgccaacggc
      781 ctgaccattt ttcggcgcag gcgcatgcca cagatcggca accatattta cgcaacgcag
      841 gtcgatggct gcggattgcg gatattcttc tttgtcgatc cagtttttat tgatcgacaa
      901 atccatcaat ttatggacgt tttcgtcgtc ccaggtctgg cagaaagtgg ctaggttctg
      961 acgagcgttg ccatcaagat ataattcatc attgataatc tggaatgcga catcatcgcg
     1021 catttcgtgc agcggaaatc gttttgactc cgcgatagta gaaatggcct ttgcgccaaa
     1081 acgtgaatcg agtagttctg agcggaaatc cgttaacagc ttctggtcca tttcgaactc
     1141 cttaaattta tttgaaggca ataaaaaagt aggatttatc cgcaatggaa gcaaggcatt
     1201 acaggcttaa t
//
LOCUS       DAZSNO010000393         1210 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZSNO010000393 DAZSNO010000000
VERSION     DAZSNO010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.399"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..308
                     /locus_tag="ACHWN2_004471"
                     /pseudo
     CDS             1..308
                     /locus_tag="ACHWN2_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            341..741
                     /locus_tag="ACHWN2_004472"
                     /pseudo
     CDS             341..741
                     /locus_tag="ACHWN2_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          349 a          261 c          303 g          297 t
ORIGIN      
        1 tgctgggcgc tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga
       61 cgcaacgact ggcagagatg gctgcggcca acagtgacgt ccccatgatg aagaatgtgg
      121 ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catgcgccat ggcgtatcaa
      181 acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa gccaagggct
      241 accgcaaccg ggagcgcttt aagctgggag tgatgttcca ctatgggaaa ctgaatatag
      301 cgttctaagc cttcccacca tgatcgggga agccccagat atgaacaggc acattcagag
      361 ttactttaaa gagcttactg aacgtctgag gtgttagagc catgactgcc gcagcattgc
      421 tggcggaaat gcctgaatcg agcagcctct cgagacgtga aatcagcgca cttgtcggtg
      481 ttgctcaggt aaaccgggac tcgggaactc tgcgcggtcg acgaaccatt ttcggtgatt
      541 gtgctgggga agaacagctc tgtacatggc ggcgcttgag gccccccccg gtttaatctg
      601 gtgataaagg cgttttacat gcgcctactt gcagccggaa acgccaaaaa agtggcactg
      661 gttgcctgta tgcgtaagtt gctgaccatt atgaacgcaa tgctcagaaa aaacgaagaa
      721 tggaatgaat cgtatttatg aaatactgga ttgtgggcca taaatattcc aaaaagatag
      781 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      841 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      901 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      961 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
     1021 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
     1081 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
     1141 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
     1201 attctgcaga
//
LOCUS       DAZSNO010000394         1201 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZSNO010000394 DAZSNO010000000
VERSION     DAZSNO010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.400"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            146..325
                     /locus_tag="ACHWN2_004473"
                     /pseudo
     CDS             146..325
                     /locus_tag="ACHWN2_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(342..1032)
                     /locus_tag="ACHWN2_004474"
                     /pseudo
     CDS             complement(342..1032)
                     /locus_tag="ACHWN2_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1111..1201
                     /locus_tag="ACHWN2_004475"
                     /pseudo
     CDS             1111..1201
                     /locus_tag="ACHWN2_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          332 a          264 c          298 g          307 t
ORIGIN      
        1 aataacactg aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg
       61 ttttacatca tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt
      121 actgtaaaaa aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt
      181 caggcaggag aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag
      241 gaaaactttg tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg
      301 gaatgcactg aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca
      361 atgaaggaac cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta
      421 cgtgccagcc gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga
      481 agattttgcc ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat
      541 cgctggtcac catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct
      601 catcattccg tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat
      661 accagggcca gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac
      721 agataagggc aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct
      781 ttttaatgtc cgtatgacgg tattgatgcc gattttcagt gtcctggcgg tatcacggcc
      841 ccctgcgccg ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc
      901 ataagagtaa gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg
      961 ggaacaacta cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt
     1021 aacggtagtc atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa
     1081 gacattacct tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg
     1141 gcagttggaa aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata
     1201 a
//
LOCUS       DAZSNO010000395         1163 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZSNO010000395 DAZSNO010000000
VERSION     DAZSNO010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.401"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            9..659
                     /locus_tag="ACHWN2_004476"
     CDS             9..659
                     /locus_tag="ACHWN2_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933684.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            631..1065
                     /locus_tag="ACHWN2_004477"
     CDS             631..1065
                     /locus_tag="ACHWN2_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR8933685.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
BASE COUNT          328 a          236 c          293 g          306 t
ORIGIN      
        1 agtaatccat gcatcgtata gacacgaaaa ccgcgaagaa ggataagttc ggcgcgggta
       61 agaacggttt tacccgtggt aacccccaga ctggcacgcc tgccaccgat ctggatgatg
      121 actactttga catgttgcag gaggagcttt gcagcgtggt ggaggcatcc ggtgccagcc
      181 tggagaaggg gcggcacgat cagttgctta ccgcacttcg cgcgctgctg ttaagccgca
      241 agaatccgtt tggcgatatc aaatcggatg gcacggtgaa aacggctctc gaaaaccttg
      301 gtttgggaga agcagctaaa aggaatgtag gtacaggggc gaatcagata cctgatatga
      361 gcctgttcgc gtcaattaat accgtaacgg ctgctgcgca aaaatttccg tctggattaa
      421 ttttacagtg cggtcagttg aatggtgccc cgaatgtatc ttcaacatac gggatgaggt
      481 tcccgatgac gttctcaaga gtaattgctg tcgtagttac attgaacgtt actggcgcgg
      541 ctgggcagcc gactgtatcg gcgacaagtg tccagaacac tggatttaat attactgtgt
      601 cgcccggttc aggatacggt tcatctgctg atgcgtatta cattgcaatg ggatattaac
      661 aaaatgtcat atttttattc tgcatcgaca aacggatttt attcgactga atttcacggc
      721 accaatattc ctgatgatgc agtggaaatc tcggaatcag agtggaaaac actgattaat
      781 gcacagagtg taacaaaaat gattacctgt ggtgagaacg gtcatcctgt cattgttgac
      841 cgtccttctc caacaccaga acaattagcc ttaataaatg atgaaaagaa atctgcactg
      901 atagcagagg caacgaatgt aatagctccg cttcaggatg cggttgattt aggtatggca
      961 acagatgatg aaacgaaact gttactggca tggaaaaaat atcgggtgct gcttatgcgt
     1021 gttaatgtag taaaacccga gtggcctatg catccaaata aatagacttg ttaatctagg
     1081 ataattgttg gaataatact cgtttttcag taggttcagg tagcctgggt ataatgctca
     1141 cttctacaat gatttcaact tat
//
LOCUS       DAZSNO010000396         1160 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZSNO010000396 DAZSNO010000000
VERSION     DAZSNO010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.402"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            115..>1160
                     /gene="cadB"
                     /locus_tag="ACHWN2_004478"
     CDS             115..>1160
                     /gene="cadB"
                     /locus_tag="ACHWN2_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR8933686.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          247 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctga
//
LOCUS       DAZSNO010000397         1156 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZSNO010000397 DAZSNO010000000
VERSION     DAZSNO010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.403"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            41..1066
                     /locus_tag="ACHWN2_004479"
                     /pseudo
     CDS             41..1066
                     /locus_tag="ACHWN2_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1039..>1156
                     /locus_tag="ACHWN2_004480"
     CDS             1039..>1156
                     /locus_tag="ACHWN2_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933687.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          323 a          283 c          268 g          282 t
ORIGIN      
        1 ccatcttaaa tgactctgag gtgtctgtta aacccgtggc gattcacaat tctcttgcac
       61 cattaactat gttcagtccc tgcgcgagtt gtacaccgat gtcatcgaag agttttactg
      121 ggttgcgctc ccgcttacga cgcaaaattc cctttcacaa taccaacccg aatggcagtg
      181 ctgggaacct gatgtcgaat gggtgcgtca gcccccgcaa gatgcgataa ccgctcctga
      241 ctttttctgc ttttaccagc caggcatgac cttcgaacaa tttgtacgtg agtttgccga
      301 atggttttca caaaaacgtc cggcggcgat gatgatcggc atccgtgcgg atgagtccta
      361 caaccgtttt gtcgccatcg ccagtttaaa taaacaacgt tttgccgacg ataaaccatg
      421 gactacggcc gcaccaggcg gtcatagctg gtacatttac cccatttacg actggaaagt
      481 ggctgatatc tggacctgat atgcaaatca tcaaaatctc tgcaacccac tgtataacct
      541 aatgtatcag gcaggcgttc ctctgcgtca tatgcgaatt tgcgaacctt ttggcccgga
      601 gcaacgacaa ggattatggc tctgtcacgt tatcgaaccg gatcgctggg ctgctatgtg
      661 cgcacgagtc agcggggtaa aaagtggcgg catttacgcc ggacatgaca atcatttcta
      721 tgggcatcgg aaaatcctca aaccagaaca tttagactgg caagaatatg cattattgct
      781 gctcaatagc atgccggaaa aaacagctga gcattaccgc aataaaatcg ccatttattt
      841 gcactggtat cagaaaaaag gcatcgaggt tccacaaacc cagcaagggg acattggcgc
      901 gaaagatatc ccctcctggc ggcggatatg caaagtttta ctcaataacg attactggtg
      961 tcgggcatta tcatttagtc ctacgaaagc gaagaactat cagcgttata acgaacggat
     1021 aaaaggaaaa cgtcaggaat gggggatact atgcaacaac gattaacgca ggatttaacg
     1081 caatttctcg ccagcttgcc ggaagatgat cgcatcaaag cgattaatga aatccgcatg
     1141 gcgatccatc aggtta
//
LOCUS       DAZSNO010000398         1155 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZSNO010000398 DAZSNO010000000
VERSION     DAZSNO010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.404"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..385
                     /locus_tag="ACHWN2_004481"
     CDS             <1..385
                     /locus_tag="ACHWN2_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933688.1"
                     /translation="VLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(386..>1155)
                     /locus_tag="ACHWN2_004482"
     CDS             complement(386..>1155)
                     /locus_tag="ACHWN2_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR8933689.1"
                     /translation="RLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTP
                     TGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSLIDIVQQGFDAGVRLSGI
                     VEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFA
                     KELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          295 a          262 c          279 g          319 t
ORIGIN      
        1 ggtgctggtg gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct
       61 ctataaatgg ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt
      121 tgttgcattt attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat
      181 tcgtaataag ccgctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctgtgc
      241 ctgtttgacg gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt
      301 ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta ggactgatat tcccgctgct
      361 ggcgcgtaaa gcgaatagca aataattatt gtggtcggtg acgctgactc tggcagatct
      421 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
      481 ggtaatagac ttggaagcct ggacgctctg tgatccagtc ttccagtaca cattctaaac
      541 gaccagtatc aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca
      601 tcaaaacggc ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa
      661 tttccagctc ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa
      721 cacattgatg attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact
      781 ctggcgttgc tgctacacat aatttcactg gtgggccgat cgccacgctg atcatatctt
      841 tttcgacgat gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgataa
      901 gagaatcgtc agttgtgagc tctactttaa tatcaggata ttcacgtgtg aaaccaacca
      961 gtaaaggcat taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg
     1021 tcggtgtcag acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg
     1081 ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc
     1141 gattgaataa gcgaa
//
LOCUS       DAZSNO010000399         1143 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZSNO010000399 DAZSNO010000000
VERSION     DAZSNO010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.405"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            305..>1143
                     /gene="ipaH1.4"
                     /locus_tag="ACHWN2_004483"
     CDS             305..>1143
                     /gene="ipaH1.4"
                     /locus_tag="ACHWN2_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HFR8933690.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSA
                     TISA"
BASE COUNT          385 a          237 c          193 g          328 t
ORIGIN      
        1 tagagcagta attctatttc agagaggagt aactccatct atcctcgata cgtactgaac
       61 gttatcgtga tatattcatc atgctttctt caccattatt cgagtatagg gagagacgca
      121 atgaccttaa ataccttttt atggctgaat taccattctc aggaataaca gcagactcct
      181 tcccatagac ggcttagggg gaaagcaata cccggagaaa gggtacaaag caaactgttt
      241 taaaattaat aagccatatt ctatttaact agaattctta tcgttgataa gaattctagt
      301 tataatgatt aaatcaacca atatacaggc aatcggttct ggcattatgc atcaaataaa
      361 caatgtatac tcgttaactc cattatcttt acctatggaa ctgactccat cttgtaatga
      421 attttattta aaaacctgga gcgaatggga aaagaacggt accccaggcg agcaacgcaa
      481 tatcgccttc aataggctga aaatatgttt acaaaatcaa gaggcagaat taaatttatc
      541 tgagttagat ttaaaaacat taccagattt accgcctcag ataacaacac tggaaataag
      601 aaaaaaccta ttaacacatc tccctgattt accaccaatg cttaaggtaa tacatgctca
      661 atttaatcaa ctggaaagct tacctgcctt acccgagacg ttagaagagc ttaatgcggg
      721 tgataacaag ataaaagaat taccatttct tcctgaaaat ctaactcatt tacgggttca
      781 taataaccga ttgcatattc tgccactatt gccaccggaa ctaaaattac tggtagtttc
      841 tggaaacaga ttagacagca ttcccccctt tccagataag cttgaagggc tggctctggc
      901 taataatttt atagaacaac taccggaatt accttttagt atgaacaggg ctgtgctaat
      961 gaataataat ctgacaacac ttccggaaag tgtcctgaga ttagctcaga atgccttcgt
     1021 aaatgttgca ggtaatccat tgtctggcca taccatgcgt acactacaac aaataaccac
     1081 cggaccagat tattctggtc ctcaaatatt tttctctatg ggaaattctg ccacaatttc
     1141 cgc
//
LOCUS       DAZSNO010000400         1100 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZSNO010000400 DAZSNO010000000
VERSION     DAZSNO010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.406"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            55..>1100
                     /locus_tag="ACHWN2_004484"
     CDS             55..>1100
                     /locus_tag="ACHWN2_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8933691.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          344 a          255 c          178 g          323 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt
//
LOCUS       DAZSNO010000401         1091 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZSNO010000401 DAZSNO010000000
VERSION     DAZSNO010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.407"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHWN2_004485"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACHWN2_004485"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR8933692.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="ACHWN2_004486"
     tRNA            complement(986..1062)
                     /locus_tag="ACHWN2_004486"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          285 a          255 c          248 g          303 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 caccggtttt a
//
LOCUS       DAZSNO010000402         1088 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZSNO010000402 DAZSNO010000000
VERSION     DAZSNO010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.408"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..318
                     /locus_tag="ACHWN2_004487"
     CDS             <1..318
                     /locus_tag="ACHWN2_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HFR8933693.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
     gene            complement(365..706)
                     /gene="symE"
                     /locus_tag="ACHWN2_004488"
     CDS             complement(365..706)
                     /gene="symE"
                     /locus_tag="ACHWN2_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFR8933694.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(927..>1088)
                     /locus_tag="ACHWN2_004489"
     CDS             complement(927..>1088)
                     /locus_tag="ACHWN2_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933695.1"
                     /translation="NIIVAIPPYNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLA
                     DALTDAAIN"
BASE COUNT          292 a          214 c          273 g          309 t
ORIGIN      
        1 ttattcgatc atcttcgcga tgaagttatg cgcctggacg cagggatcac gcaggaagta
       61 ttgaagctat atattgcgtt taaagcggaa acgaattttg ttgatgttgt gccgcaaaaa
      121 agccgactgc gattgtcgct taatatgcag tttcatgaac tggtcgatcc gaaaggtatt
      181 gccaaagatg tgacaaatgt tgggcgctgg ggtaatggcg atgtagaaat tggtttcagc
      241 gacctcgcac aacttcctta cattatggga ttaattcgtc aggcatttga aaaacagatg
      301 gagagcgcgt tggtataacg cggcgaataa tacccggtag cactggcgct accgggtcaa
      361 tgtatcaggc gactttctgt ttacctgtaa ttaccccaat aaattcctgt acctgccttt
      421 gtttacgcgc cgacagctta cacaccttgc gtagtgactg catcagttcg ctctcttcgg
      481 cggcggcggg ttgggcggtg aggacaatac agccttccat cactttgaca tctaccgccg
      541 tgccggtggc aaaaccggcg gcttccagcc actgaccttt cagggtgatg gcgggaatac
      601 ggctgtaatc cgggtaacga ctcgcataac tcacggttaa ttgacgatta tttgccgggg
      661 agacttctgc ttctaacggt tgtgcaatag aatgcgtgtc agtcatgatt actattctct
      721 ataaatagtg attgtgatta gcggtgcgag tgtgttggcg cacatccgca ccgtgctaaa
      781 tacctggata catcatcagt aaatacaggg aaagtccagc taaaaattaa tgaaaataag
      841 atgaaaatta gcgtgaaata taaattttgg tgatgtgaat aaagcggcta tttagccgct
      901 ttattggttt tgtgagggcg agggaattag tttatcgctg cgtcagtgag tgcgtctgca
      961 agatgcagtt gggttttatg ataaatccca atataagaaa taagaccatt gcatactgct
     1021 tcaatagaat ttatcttatc aaatattgct tgttgttcat tatagggggg aatcgcaaca
     1081 ataatatt
//
LOCUS       DAZSNO010000403         1084 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZSNO010000403 DAZSNO010000000
VERSION     DAZSNO010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.409"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(153..419)
                     /gene="ospE2"
                     /locus_tag="ACHWN2_004490"
     CDS             complement(153..419)
                     /gene="ospE2"
                     /locus_tag="ACHWN2_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HFR8933696.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(528..658)
                     /locus_tag="ACHWN2_004491"
                     /pseudo
     CDS             complement(528..658)
                     /locus_tag="ACHWN2_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            656..739
                     /gene="tnpB"
                     /locus_tag="ACHWN2_004492"
                     /pseudo
     CDS             656..739
                     /gene="tnpB"
                     /locus_tag="ACHWN2_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          313 a          188 c          237 g          346 t
ORIGIN      
        1 taagttggca gcattaccgt atcgaggata gatggagtta ctcctctctg aaatagaatt
       61 actgctctac tttttctgtt tccagaaatt attagtaaca aaccctgata tattcttttg
      121 aaaatggagc ttaaacgagt ctgaaattaa cttcagaaat cagaataccg ttggctttgt
      181 ttttcatcaa ttccggcttc atttatcttt gtcaaccatt gcatttctgt agcagtgaat
      241 ttcaacgttt tcgtccggtt ggcagcaggt atttgtaatt cgtatatagc tgctttttta
      301 ttgaaaacag tatatccatc tgtagtaaca gtagaagata ataatacagg atttgatact
      361 tcaaggatga tattttcttg tttttgtgga agacaaggga atatagtttg tgtaagcata
      421 ttacttcctt atgttatatt ttggtaaaaa atttattttt ttacattata gtcggctaaa
      481 tgaagtttac tgtaaaaaaa cgatactttt tcgggtggcg cttccaatca gtagaatatg
      541 tgtttttcaa tgaaggagcc gatgactttt cgtggatttc cacggaacgc gagaagcaga
      601 ttgttttacg tgccagccgc ttgatgcggg tgcggagggt caggttgtta cgttcaagat
      661 atagcatgtg ctggatgaag atcccttttc tggtcacctg tttatctttc gtggccgacg
      721 gggtgacact gttaaaatgt aatgaacgca tcccacacaa ttaaacacgg agtagtatga
      781 tggttaaaag ggtgaaactg gcaggtgtgg agcctcgcag ctttccggca tcatactgcc
      841 caatacggga tgcataaagc gtcctcagac tggagactcc acaaatgaaa tatacaccgg
      901 ttggcgttga tatggcaaaa catgtcattc agattcactt catcaatgag cacatgtcag
      961 cgccagtgat ataagacggt aatggggatg cggacgattt cctggactct cagaagatga
     1021 aacatgttta ctgaagaaca aaaaattcgc gctattgagc tttatttcaa atatggtaag
     1081 aagc
//
LOCUS       DAZSNO010000404         1073 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZSNO010000404 DAZSNO010000000
VERSION     DAZSNO010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.410"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(17..1000)
                     /gene="kdgT"
                     /locus_tag="ACHWN2_004493"
     CDS             complement(17..1000)
                     /gene="kdgT"
                     /locus_tag="ACHWN2_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR8933697.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
BASE COUNT          288 a          310 c          248 g          227 t
ORIGIN      
        1 gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt
       61 tgactttacg tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg
      121 ccgtcgctac cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg
      181 caatcagcac aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag
      241 tgccatcgcc accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa
      301 ttactgccac acccaacagg atccccagca aaccagtctg ggcaatcaca gtcaaatcaa
      361 tggtattgcc cagcgcgaag gcaaagaatg gaatcagcgt ttgcaccgct ttgctgaaaa
      421 attctcgtaa ttcagggtca agattcccca gggcaaagcc caccaggaac ggtaatactg
      481 cgccgacgaa aacatgcggt tcaaacgagg caatcccggc agtgcccaga ataatcatcg
      541 tcatgagcgg cccggactcc aacgacatca acacaaatgc cccagcttct tctttcgtac
      601 cgtactgctg catgatggaa gcgtaaagtc cgccgttggt catatccatc gccgccacca
      661 gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac accatgttcc ggaataatgc
      721 gcgaggcaat tgccgcaacc acccacgcga cggcaatttt agttaccacc agcgtaccgg
      781 atttacgcag taccgttccc gtcgcgctta attttattga cgcccccatg caaaaaaacc
      841 acaccgccag aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat ccaaaatatt
      901 tccccgcccc cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg acgagcatca
      961 tcccccccgg gattttctca atcgagcgtt ttatctgcat ttccatcacc tgtcggcata
     1021 tttttaaggg ctgatgcgta attcctcaat ggagcatcag tgttgatgga aac
//
LOCUS       DAZSNO010000405         1069 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZSNO010000405 DAZSNO010000000
VERSION     DAZSNO010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.411"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(348..1043)
                     /gene="aqpZ"
                     /locus_tag="ACHWN2_004494"
     CDS             complement(348..1043)
                     /gene="aqpZ"
                     /locus_tag="ACHWN2_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFR8933698.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          300 a          322 c          250 g          197 t
ORIGIN      
        1 gatttttaat cctatctgag tgtttagcat tgttgcgctc ctttcatgtg caatgccata
       61 taaagccact ggcggaggga gcgcgcaggg ggcagccagt cgccgccgcc ccctgcaccc
      121 ccgggctctg gcgaacaaaa tcgccgctgc gcggtacctt cagcttatcc ccttcggcaa
      181 ccgggtcggg cgcgaggtaa cgtcctgtaa gacgcgccct cagcccacat ccatgtgggc
      241 tgccccggcc ttcggggaac gcttcagcga ttttgacgcc accaataccc gtgcggtttg
      301 taatgtcgaa gataaaacaa ccagggcaga taaaatgcca atgctaatta atcacgcttt
      361 tccagcagag tccggtaaat cagaccaccg ataatgccgc cgacaattgg caccacccag
      421 aagaaccaca gttgttctaa tgcccagccg ccctggaaga tagcaaccgc ggtgctgcgc
      481 gccgggttaa cagaagtgtt agtcaccgga atactgatta agtgaatcag ggttaaggcc
      541 agaccaatag cgatcggcgc aaaacctgcc ggagcgaatt tgtcggttgc accgtggatc
      601 actaacagga agcccgcact caataccagt tcaactacca gcgcggaaag catggaataa
      661 ccgcctggtg aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa
      721 cccgttttac cactggcaat taaatacagc agcgccgctg caacaatacc gccgacaacc
      781 tgggcaatta cgtagccaac gacttctttt gccggaaaac gtccgccagc ccataaacca
      841 atagtgaccg ccgggttaaa atgaccacca gaaatatgac caacagcgaa ggccatcgtc
      901 agaacggtca aaccaaatgc cagcgccacg ccggcaaaac caatgcctaa ttccgggaag
      961 cctgcggcca gtacagcact accacagcca ccaaaaacaa gccagaaagt accaaaacat
     1021 tcagctgcta attttctgaa catatccacc tcaattaaaa attgaccct
//
LOCUS       DAZSNO010000406         1068 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZSNO010000406 DAZSNO010000000
VERSION     DAZSNO010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.412"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..560
                     /locus_tag="ACHWN2_004495"
     CDS             <1..560
                     /locus_tag="ACHWN2_004495"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFR8933699.1"
                     /translation="TKGDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDN
                     YQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKS
                     GLTGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMV
                     NEVETALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="ACHWN2_004496"
     CDS             787..>1068
                     /locus_tag="ACHWN2_004496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HFR8933700.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DAZSNO010000407         1066 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZSNO010000407 DAZSNO010000000
VERSION     DAZSNO010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.413"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(570..749)
                     /locus_tag="ACHWN2_004497"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="ACHWN2_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="ACHWN2_004498"
     CDS             843..>1066
                     /locus_tag="ACHWN2_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8933701.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAZSNO010000408         1058 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZSNO010000408 DAZSNO010000000
VERSION     DAZSNO010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.414"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(12..705)
                     /gene="ospZ"
                     /locus_tag="ACHWN2_004499"
                     /pseudo
     CDS             complement(12..705)
                     /gene="ospZ"
                     /locus_tag="ACHWN2_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            761..913
                     /locus_tag="ACHWN2_004500"
                     /pseudo
     CDS             761..913
                     /locus_tag="ACHWN2_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          317 a          210 c          178 g          353 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 ggaagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgccatactt ctggttatac cagagatata cttcttgtca tatatgtttg gtgattcatt
      601 aaagtaacca tgttcaactc taggataaat atttctcact atatattcag tatttgcagt
      661 atttattgga aatacgttct taatattctt gatgggacta atcattttaa tctctatact
      721 caaatataaa cattaccatg aacaatataa ttgttcctaa gtggaatgtc tccacggaga
      781 acactttatc tatcgggaaa tagtgcgggc aacagtgttt aattatatcg agtgcaatta
      841 caatcgctgg cgacggcacc gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca
      901 gaacctcgct taggcctgtg tccatattac gtgggtaggc tcacagagcc tcacttacga
      961 ctgcgtccat attatgtggg taggatcaat aaagacgttt tatatgtgtc tgcttgcagc
     1021 cagaaaattt ataccctgca ggtatcataa acatgctt
//
LOCUS       DAZSNO010000409         1025 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZSNO010000409 DAZSNO010000000
VERSION     DAZSNO010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.416"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            103..>1025
                     /locus_tag="ACHWN2_004501"
     CDS             103..>1025
                     /locus_tag="ACHWN2_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8933702.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITILEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEHSLADAVTAWFPENKQSDVSQIWHA"
BASE COUNT          341 a          210 c          180 g          294 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaatactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcatc
      661 cccccctttc cagataagct tgaagggctg gctctggcta ataattttat agaacaacta
      721 ccggaattac cttttagtat gaacagggct gtgctaatga ataataatct gacaacactt
      781 ccggaaagtg tcctgagatt agctcagaat gccttcgtaa atgttgcagg taatccattg
      841 tctggccata ccatgcgtac actacaacaa ataaccaccg gaccagatta ttctggtcct
      901 cgaatatttt tctctatggg aaattctgcc acaatttccg ctccagaaca ctccctggct
      961 gatgccgtga cagcatggtt cccggaaaac aaacaatctg atgtatcaca gatatggcat
     1021 gcttt
//
LOCUS       DAZSNO010000410          971 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZSNO010000410 DAZSNO010000000
VERSION     DAZSNO010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.418"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..950)
                     /locus_tag="ACHWN2_004502"
     CDS             complement(<1..950)
                     /locus_tag="ACHWN2_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR8933703.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPYLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDESMERFSWHVADKGFRMVRYYVF"
BASE COUNT          223 a          286 c          242 g          220 t
ORIGIN      
        1 aaaaaaacgt agtacctcac catacggaac cccttatccg ccacatgcca ggagaacctt
       61 tccatggact catctccact catcaacagg tattcttccc gttttgttcg gtgactgttg
      121 taacgcagac cgatttcatc ctgaccggca taatgctcca gacgactcat cggcaccggt
      181 ggctttttca ggtaagagcc aaagtacacc gccacatggg tggcattatc cattacccgg
      241 gatacgttga cattccagcc acggcggtaa tgcgtgtcca ggaagcgatt ccattcccgt
      301 ttactgcttc cttctgctgc cagcgcatcc ggcatcacca ggtaagggta tttccgtgac
      361 agcaaccgtg ttatccggta gcgccacatg ctcatcacct tacgggcgta aaaatgaaga
      421 tttttccagg tgtggcccga cgtcacacca ccggcagttg tcgataaatg gatatgcgga
      481 tgccactgct ggtcacgccc ccatgtgtgg atcaccgtga atatccccgg ctccacatct
      541 gcctgatggc agatttccag tatcacatcc gctgcaatgc ggctcatctc tgccagtaac
      601 caccggttgt ggaacaccag ggaccagtac tggcagggaa gtgtgaacac aatatgctgc
      661 cacgggcagt aggggaccag gctcagcaga tactgtatcc actgtgcgcc agccttcacc
      721 ccgcagtgcg ggcaggagcg gcttttacac cggaagcaga ccttttttgt atggcaacag
      781 tccggtgatg aacaacacca ctgcgtgtag cccatcagtg tggtcccgca ggccatgatt
      841 tttgtcactg attcaattac caccggacgg actgcccctt ccggttgttt ctccagccag
      901 ttaagccagc ggtttccctg ctgaaaaata tcggcaaaac gggggagcat cagaagggcg
      961 gggcgactcc g
//
LOCUS       DAZSNO010000411          952 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZSNO010000411 DAZSNO010000000
VERSION     DAZSNO010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.419"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(299..781)
                     /gene="sseA"
                     /locus_tag="ACHWN2_004503"
                     /pseudo
     CDS             complement(299..781)
                     /gene="sseA"
                     /locus_tag="ACHWN2_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT          226 a          250 c          224 g          252 t
ORIGIN      
        1 ttaccggtta gaatttttaa ttttgagcag gatttgttta tatttgtctg aattcggact
       61 aataagccag gttattttat taccgaacac ggcggaaagg gccggtgtcg ataatacgct
      121 aaataaacaa taattactct cttttgcttg acaaaaagag agttactggt gagtattgtt
      181 ttgctgtgaa gcacctgcgt tgctcatgta acacaagtca acctgctgga aaggccgcga
      241 accagaccag caataaaaaa acgccaaatt cggcggtttt ttattgcctg taatatactt
      301 atttcactgg ctcaaccggt aaatctgccc gcgcgcccca ttcactccat gcgccgtcgt
      361 acagtttcac gtttggcaca tccagcgtcg cgagtgctaa caaaaccacg gctgccgtta
      421 caccagagcc gcagctgacg ataatcggtt tgtcatagct gacgccgcga ccaaaaaata
      481 tcgcatccag ttcatcggtc gtttttagtt cgccttcgcg caccagttcc gtccacggaa
      541 cgttcaatgc accgggaata tgtccgcgac gtaaacctgg gcgaggttca tcaacttctg
      601 cgttaaaacg tgcagccggg cgggcatcaa taatttgcgc cgtattttca tggcttgcca
      661 ataacacatc ggttactttc accactgctt caggattaaa cgcggcgtta aactctcctt
      721 ccggcagctc tactgcacct tcttctaata gcaggtcatc gcgctgccag cctgcaagtc
      781 cccgttactt tattcgtacc ccttataatg gggtgttagc cagccagacc cggcatgatt
      841 actgccccca gtcgtccatg atccgggggg ggatgtcacc gggtctggtg gggcgctggt
      901 aaccgctaat aggggtcagg tcaggcactt ttgccgggac cgtctgtaac gt
//
LOCUS       DAZSNO010000412          951 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZSNO010000412 DAZSNO010000000
VERSION     DAZSNO010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.420"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>951)
                     /gene="mxiD"
                     /locus_tag="ACHWN2_004504"
     CDS             complement(<1..>951)
                     /gene="mxiD"
                     /locus_tag="ACHWN2_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR8933704.1"
                     /translation="VIPGVATVVERLLNNGKALSNRQAQNDPMPPFNITQKVSEDSND
                     FSFSSVTNSSILEDVSLIAYPETNSILVKGNDQQIQIIRDIITQLDIAKRHIELSLWI
                     IDIDKSELNNLGVNWQGTASFGDSFGASFNMSSSASISTLDGNKFIASVMALNQKKKA
                     NVVSRPVILTQENIPAIFDNNRTFYVSLVGERNSSLEHVTYGTLINVIPRFSSRGQIE
                     MSLTIEDGTGNSQSNYNYNNENTSVLPEVGRTKISTIARVPQGKSLLIGGYTHETNSN
                     EIISIPFLSSIPVIGNVFKYKTSNISNIVRVFLIQPREIK"
BASE COUNT          298 a          185 c          150 g          318 t
ORIGIN      
        1 tcctttatct cacggggttg aatcagaaaa acccttacaa tattactgat attacttgtt
       61 ttatacttga acacattacc gattacagga atagaggaga gaaaaggaat agatattatt
      121 tcgttagagt tcgtttcatg cgtataacca cctatcaaca aacttttgcc ttgtggaact
      181 ctcgctattg tactgatttt ggtccttcca acttctggaa ggacagatgt attttcatta
      241 ttataattgt aatttgactg actatttcct gttccatcct ctatcgttaa agacatctca
      301 atttgtcccc gagatgaaaa gcgaggtata acatttatca atgttccata cgtaacatgc
      361 tccaacgaag aattgcgctc accaactaat gacacataaa aagtcctgtt attatcaaat
      421 atagccggaa tattctcttg tgtcaaaatc accggccttg aaactacatt tgctttcttt
      481 ttctgattta atgccataac tgaagcaata aacttattgc cgtccaaagt gcttatcgat
      541 gctgatgagc tcatattaaa tgacgcacca aacgaatcac caaatgaagc cgttccttgc
      601 caattaacac caaggttatt taattcagat ttatcaatat cgattatcca taatgataat
      661 tctatatgtc gtttggctat atctaactga gtgataatat cgcgaataat ctgtatttgc
      721 tgatcattac ctttgactaa tatagaattg gtttctggat atgcaataag tgatacatcc
      781 tctaatatag aactattggt aactgaacta aatgaaaagt cattactatc ctcagatact
      841 ttctgtgtaa tattaaaggg tggcatcgga tcattttggg cttgtctgtt actcagagcc
      901 ttaccattat ttaataaacg ttctacaaca gtagccaccc ctgggatgac a
//
LOCUS       DAZSNO010000413          941 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZSNO010000413 DAZSNO010000000
VERSION     DAZSNO010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.421"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..213
                     /locus_tag="ACHWN2_004505"
     CDS             <1..213
                     /locus_tag="ACHWN2_004505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933705.1"
                     /translation="YCKKTRFVTACAQDISQQSATGKKNDCLVIFDSGKRRFKRKQNI
                     FCVDNSVFTRVLILWYEQKKKNTTRG"
     gene            342..797
                     /gene="csgB"
                     /locus_tag="ACHWN2_004506"
     CDS             342..797
                     /gene="csgB"
                     /locus_tag="ACHWN2_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR8933706.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            838..>941
                     /locus_tag="ACHWN2_004507"
     CDS             838..>941
                     /locus_tag="ACHWN2_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HFR8933707.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
BASE COUNT          299 a          165 c          230 g          247 t
ORIGIN      
        1 tactgcaaaa agacgaggtt tgtcacggct tgtgcgcaag acatatcgca gcaatcagcg
       61 acgggcaaga agaatgactg tctggtgatt tttgatagcg gaaaacggag atttaagaga
      121 aaacaaaata ttttttgcgt agataacagc gtatttacgc gggttttaat actttggtat
      181 gaacaaaaaa agaaaaatac aacgcgcggg tgagttatta aaaatatttc cgcagacata
      241 ctttccatcg taacgcagcg ttaacaaaat acaggttgcg ttaacaacca agttgaaatg
      301 atttaatttc ttaaatgtac gaccaggtcc agggtgacaa catgaaaaac aaattgttat
      361 ttatgatgtt aacaatactg ggtgcgcctg ggattgcagc cgcagcaggt tatgatttag
      421 ctaattcaga atataacttc gcggtaaatg aattgagtaa gtcttcattt aatcaggcag
      481 ccataattgg tcaggctggg actaataata gtgctcagtt acggcaggga ggctcaaaac
      541 ttttggcggt tgttgcgcaa gaaggtagta gcaatcgggc aaagattgac cagacaggag
      601 attataacct tgcatatatt gatcaggcgg gcagtgccaa cgatgccagt atttcgcaag
      661 gtgcttatgg taatactgcg atgattatcc agaaaggttc tggtaataaa gcaaatatta
      721 cacagtatgg tactcaaaaa acggcagttg tagtgcagag acagtcgcaa atggcaattc
      781 gcgtgacaca acgttaatta ccattcgact tttaaatcaa tccgatgggg gttttacatg
      841 aaacttttaa aagtagcagc aattgcagca atcgtattct ccggtagcgc tctggcaggt
      901 gttgttcctc agtacggcgg cggcggtaac cacggtggtg g
//
LOCUS       DAZSNO010000414          938 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZSNO010000414 DAZSNO010000000
VERSION     DAZSNO010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.422"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            100..293
                     /locus_tag="ACHWN2_004508"
                     /pseudo
     CDS             100..293
                     /locus_tag="ACHWN2_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            436..657
                     /locus_tag="ACHWN2_004509"
                     /pseudo
     CDS             436..657
                     /locus_tag="ACHWN2_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(678..740)
                     /locus_tag="ACHWN2_004510"
                     /pseudo
     CDS             complement(678..740)
                     /locus_tag="ACHWN2_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          290 a          222 c          210 g          216 t
ORIGIN      
        1 tttagctgaa cgggccatat aaccacctga aagacaatga cattgcctgt ttttataacg
       61 gtaattgcag accatgacaa accgcagccg tcaggctgcc tactcgagtc acaaggctct
      121 ttgtgtccga tatgccggca gcgcattacc catgaaactg gctggaacgt acaccataag
      181 gtgaagaaag ttatgggggc ggtgaagaat tatctaacct tgtcttgtta catccaaatt
      241 gccatcgaca gttacacagt tgtgaagccg gctctctcaa aaagggctta taaaggcttg
      301 agcggtgtac cgggaaaccg gtatgcaccg ttcttagggg agggcagtcc agcaatgaac
      361 tgtccttacc cgacaaacat ccaaaacgaa cggaacgtgc tggaatccgc atataacccg
      421 ttgtaaagtg aggaaatgga cacctcactt gctcatgaga acgcccgcct gcgggcactg
      481 ttgcagacgc aacaggacac tatccaccag atggctgaat acaaccgcct gctctcacag
      541 cggatggcgg cttatgcttc cgaaatcaac cgactgaagg cgctggttgc gaaactgcag
      601 cgtatgcagt tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca
      661 ttttcacggg cgatctacgc cacacaggct ctgggttgca gtgactgctc cattaaggcg
      721 attttgaact cataagggaa gtaacaaaaa aactgcaaaa gtacaataat tatatatggc
      781 caaactaacc tctattaaaa aacatcagtt tttcacactt agttgagctt catgaaaaaa
      841 tcatcgagga tttcattgca gaatacacat tccactaact ggaagcactc cataagagga
      901 atcagagaaa gtatcgtttt tttatagtaa aattcatt
//
LOCUS       DAZSNO010000415          937 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZSNO010000415 DAZSNO010000000
VERSION     DAZSNO010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.423"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(20..532)
                     /locus_tag="ACHWN2_004511"
     CDS             complement(20..532)
                     /locus_tag="ACHWN2_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933708.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(671..>937)
                     /locus_tag="ACHWN2_004512"
     CDS             complement(671..>937)
                     /locus_tag="ACHWN2_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR8933709.1"
                     /translation="SGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGIAYV
                     SGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT          256 a          225 c          173 g          283 t
ORIGIN      
        1 aattaactaa tcatctgcat tattctgcgt agatataagc ctgggtacgg atggtgtaag
       61 agtctggctc aacaacctgt tctttcatat gggaaacgac gcgataggca ccacctgttc
      121 cagtattttt cagcatcata taacgcttac cgccaatctc ttcggaggca gctgaaggag
      181 gatcctgctc aagcgttgtc agggaaactt tcagtttatg gtccgggtta ttgtcaccag
      241 aaattactgc atccgctgaa gtactgctct ccgcataaat atcatccggg aaagttacag
      301 catagtcgtg gctctgccca tcagatgcgc caacaatacc tgttgccaaa ggcgtccatg
      361 cagttaacgt accgctatat acccctttca ctggttgaat ggtattatta acaacgatag
      421 gcttggtcaa agtaagatta gctgacgctt cagtaccctg gcttactaaa gttcccgcct
      481 gagaagcaaa agctgtcccc gccagcatga ttgctaacaa tgttttcttc attatatttt
      541 ctccatgatt ttcatttaca ctgcaaaatg cagtttcttt ctttgcattt acgtgctatc
      601 gcgaaaatgc ttccaccaaa taatggaaac atcttcacaa aaataaatta tgagtttctt
      661 aatttagaaa ctataaacat gtcgccgcta tctggtataa ccctgatgca cttttcttcg
      721 caggaagttg gtattttgcg atacaggatt gatctttaga ataaccccat ttcacattaa
      781 actcgccttc cgcactcaag ccagaaacat aagcaatacc atcttcacct acaatgccgg
      841 ccatattttc agtgtctttg ctataactaa tcacagcgcc gaaggggatg ggtttaccct
      901 ctttctgttt taaggttact aatcctttat ccccgct
//
LOCUS       DAZSNO010000416          931 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZSNO010000416 DAZSNO010000000
VERSION     DAZSNO010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.424"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            265..867
                     /gene="fimB"
                     /locus_tag="ACHWN2_004513"
                     /pseudo
     CDS             265..867
                     /gene="fimB"
                     /locus_tag="ACHWN2_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
BASE COUNT          280 a          163 c          204 g          284 t
ORIGIN      
        1 tgcattaatg atgagggtta ttattagatt cgtatcagat tgataaatat ataaaggtac
       61 atagcatgca agagcatggc gtttgtatgg caaagttatt ataagtaact atcgctactc
      121 catttaagtt cgctcagaag aactggtcca cttacgtaag ttattaagca aacgttcgct
      181 tttataaaca taatcaggat aaaaatgttg gattattgct aacccagcac agctagtgcg
      241 cgtctgtaat tataagggaa aacgatgagg aataaggctg ataacaaaaa aaggaacttc
      301 cttacccata gtgaaatcga gtcactcctt aaagcagcaa ataccgggcc tcatgcaaca
      361 cgtaattatt gtctgatttt gctttgtttt attcatggtt tccgggcgag tgaaatttgt
      421 cgattgagga tttcggatat tgatcttaaa gcaaagtgta tatatatcca tcgattaaaa
      481 aaaggctttt caacaacgca cccgctattg aacaaagaag ttcaggcttt aaaaaactgg
      541 ttgagtatcc gtacttctta tccgcatgct gagagcgagt gggtattttt atcacgtaag
      601 gggaatccgc tttctcggca acagttttac catattatct cgacttctgg tggtaatgcc
      661 gggttgtcac tggagattca tccgcacatg ttacgctatt cgtgtggttt tgctttggcg
      721 aatatgggaa tagatacgcg acttatctag gattatcttg ggcatcgcaa tattcgtcat
      781 actgtctggt ataccgccag caatgcaggg cgtttttacg gcatctggga tagagccaga
      841 ggacgacagc gtcatgctgt tttatagaga tagttgagat accagggatg gtgttaaaaa
      901 ctacattatt cttacgatat atcaaaaatg a
//
LOCUS       DAZSNO010000417          923 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZSNO010000417 DAZSNO010000000
VERSION     DAZSNO010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.425"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..200
                     /locus_tag="ACHWN2_004514"
                     /pseudo
     CDS             1..200
                     /locus_tag="ACHWN2_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..>923
                     /locus_tag="ACHWN2_004515"
     CDS             300..>923
                     /locus_tag="ACHWN2_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR8933710.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          293 a          217 c          205 g          208 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatctgaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aag
//
LOCUS       DAZSNO010000418          923 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZSNO010000418 DAZSNO010000000
VERSION     DAZSNO010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.426"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>923
                     /gene="yfdF"
                     /locus_tag="ACHWN2_004516"
     CDS             <1..>923
                     /gene="yfdF"
                     /locus_tag="ACHWN2_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFR8933711.1"
                     /translation="GKEGISTCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSN
                     DTDHITIGIDNQEPIELPANYKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDG
                     DPGFIFDKISHKLRHTVTEFDESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLS
                     LGYYDQDDYHIQRGIRINGDSLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVN
                     ELNGERLREPNEVFKRLGRAIRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKY
                     KDFKNLEDKVEKRLQNRQTKYQNEINQLSAL"
BASE COUNT          354 a          160 c          169 g          240 t
ORIGIN      
        1 tggtaaggaa ggaataagca cctgtattaa acatctactt gagcttataa aaaacgccat
       61 acgagtaaac gacgatcctt atagatctaa tattaataat tcctcagtta cttatattga
      121 tattggctcc aatgatacag accatattac tattggtatc gacaaccaag aaccaataga
      181 attacctgcg aactataaag acaaagaact cgtccgtact atcattaatg acaacatagt
      241 tgagaagact catgatatca ataacaagga aatgatcttc agcgcattaa aagaaatata
      301 tgatggagat cctggtttta tcttcgataa gatatcacac aaactcagac atacggtaac
      361 ggaatttgat gagagcggga aaagcgaacc aacggactta tttacctggt acggtaaaga
      421 taaaaaaggc gactctctcg ctattgtaat taaaaataaa aacggaaatg attacttatc
      481 tctcggttac tacgatcagg acgactacca cattcaaaga ggaattcgta ttaatggtga
      541 tagtctcacc caatattgta gtgaaaacgc caggagtgct tcagcgtggt ttgaaagcag
      601 taaagctatc atggcagaat catttgcaac tggttccgat catcaggttg taaacgagct
      661 caacggggaa agactgagag aacctaacga agtttttaaa cgtttaggtc gagcaataag
      721 atataatttt caagtggacg atgcaaaatt tagacgcgat aatgtaaaag aaataatttc
      781 tactttattc gctaacaaag ttgacgttga ccatcctgaa aataaataca aagactttaa
      841 gaatctcgaa gataaagttg aaaaaagact tcaaaatcgc cagacgaaat atcaaaatga
      901 aattaatcaa ctatctgcac tag
//
LOCUS       DAZSNO010000419          917 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZSNO010000419 DAZSNO010000000
VERSION     DAZSNO010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.427"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..91
                     /locus_tag="ACHWN2_004517"
     CDS             <1..91
                     /locus_tag="ACHWN2_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933712.1"
                     /translation="REIQKTTQIIPSETTLLEDEKSLVSYLNY"
     gene            110..>917
                     /gene="mxiC"
                     /locus_tag="ACHWN2_004518"
     CDS             110..>917
                     /gene="mxiC"
                     /locus_tag="ACHWN2_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR8933713.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLK"
BASE COUNT          332 a          121 c          183 g          281 t
ORIGIN      
        1 aagagaaata caaaagacta cacaaataat cccatctgaa actactttgc tggaagacga
       61 aaaatcattg gtttcatact taaattacta actataaagt aggtgatgta tgcttgatgt
      121 taaaaataca ggagttttta gctctgcatt cattgataag ttgaatgcaa tgacaaattc
      181 agatgatgga gatgagactg ctgatgcaga gcttgattct ggcttggcta atagcaagta
      241 tattgactca tctgatgaga tggcttccgc tctttcgtca tttataaaca gaagagacct
      301 tgagaaactg aaaggaacaa atagtgatag tcaggaacgt attttagatg gggaagaaga
      361 tgaaattaat cacaagattt ttgatttaaa gagaacgtta aaagataacc ttcctctaga
      421 tcgggatttt atagacagac taaagagata ttttaaagat ccaagtgatc aagtcttagc
      481 attaagggaa cttttgaatg aaaaagatct tactgctgaa caagtcgaat tattaactaa
      541 aattattaat gagataatat caggtagtga aaaaagtgtt aatgctggaa taaattcagc
      601 tatacaggct aaattatttg gcaacaaaat gaaacttgaa ccacagcttt tgcgtgcatg
      661 ttatcgtggt tttatcatgg ggaacatatc aacaacagat cagtatatag aatggcttgg
      721 taattttggt tttaatcaca gacatacaat tgtgaatttt gtagagcagt cactgattgt
      781 agacatggat tctgagaaac cgagctgtaa tgcttatgag tttggttttg tgttatctaa
      841 attaattgca attaagatga ttagaacttc agacgtaatt tttatgaaga aactggaatc
      901 ctcaagcttg ctaaaag
//
LOCUS       DAZSNO010000420          916 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZSNO010000420 DAZSNO010000000
VERSION     DAZSNO010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.428"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..92)
                     /locus_tag="ACHWN2_004519"
     CDS             complement(<1..92)
                     /locus_tag="ACHWN2_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_169729183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR8933714.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATR"
     gene            158..916
                     /locus_tag="ACHWN2_004520"
                     /pseudo
     CDS             158..916
                     /locus_tag="ACHWN2_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          249 a          196 c          217 g          254 t
ORIGIN      
        1 cgtgttgcaa cgctggcaac agaataaccg cgatcaacaa cctgtttgac tgcttcagtt
       61 ttaaactctt cgggataacg cttaccgctc atgggcacct ctctttaagc catcttaaat
      121 gactctgagg tgtctgttaa acccgtggcg attcagtgag cgcattctga ccgaccgtga
      181 aaatggtgaa aaaggcgata attctcaaga tacctcttat gcccgtgtag gcgtgaaagg
      241 tgaaactcag atcaaccctg aaatgactgg ttacggtcag tttgaactag acctggaagc
      301 atcaaacaga cacaaccctg accaaactcg tttggcatat gctggtttga gttacaaaga
      361 ttttggttca ttcgactaca gtcgtaatgt cggtgtcgcg tatgatgcag aagcatttac
      421 tgatatgttt gttgagtggg gcggtgactc atgggctggt actgacctgt tcatgaccaa
      481 ccgtaccaac ggtgttgcaa cctatcgtaa cacagatttc tttggcatgg ttgaaggtct
      541 aaattttgct ctgcaatatc agggtaaaaa tgaaggtaca ggcaactaca aagcaaatgg
      601 cgatggccat ggtttatctg caacttacac tattgacggt ttctcatttg ctggtgctta
      661 tgcaaactct gaccgtactg attggcagtc tggtgatggt aaaggcgaaa gagccgaagt
      721 ttgggcgttg tcaacaaaat atgacgctaa caatgtttat gctgctgtga tgtacggtga
      781 atctcacaac atgaattccg acgacggtga tgttgtgaac aaaacacaaa acttcgaagc
      841 tgttctgcaa tatcagttcg actttggtct gcgtccatcc atcggttact cctattctaa
      901 agcactggac gtagca
//
LOCUS       DAZSNO010000421          915 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZSNO010000421 DAZSNO010000000
VERSION     DAZSNO010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.429"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(38..226)
                     /locus_tag="ACHWN2_004521"
     CDS             complement(38..226)
                     /locus_tag="ACHWN2_004521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933715.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            complement(523..761)
                     /locus_tag="ACHWN2_004522"
                     /pseudo
     CDS             complement(523..761)
                     /locus_tag="ACHWN2_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          244 a          176 c          211 g          284 t
ORIGIN      
        1 atccgtttta ttgaatcgga gaacaaactg tgagctatca gcctggtggt ctggttttgc
       61 ctgatttaag ccgccgtggg tatttgtcca agaatgcttc aagctccaat cgttttggcc
      121 tggtgaatgg agatgggtta agcaaagact ctcgttgctg gtgtactttt tcgcgctggc
      181 gctcaagcat ggataatgcg attgcctgta gttcttcgtt agtcattgtc tgcacctgga
      241 aagcaattta ccggagtacc tcacactacg gcaaaaatgt tttattgttg caacttgcgt
      301 aattattcag gtcagtttgg aacgcactgt ataaacataa ataacctgat actgttgtgt
      361 gttttcaaag aaaacacagg tatttttcac ataaaccttc cccttttcat attcgaccac
      421 ttaatcaaaa aacggtttta tacttgttgc ccccactggg gttaaatgtg agacatgtcg
      481 cactttgtgc catgtgtcct cgctgatttt aggcgcggtt cattcattct ttctgttggc
      541 ataaagtatg gtatcggttg ggcgaatgaa agggactcgg gtcatggtca gacaatacct
      601 gaggcgttaa ttcgttgaaa gagattgtga atgggggaat aactgagtct taactgctgt
      661 gcgatagccc ggacagatag ttgctcgtcg aatctgagac gtaatgattc tggcaaataa
      721 gtgcgaaagc cggtgatatc tagtgttgtt tgagagttca tcgttcgcgt ccattaatac
      781 agatggacgc gattgttgcc agacaggata attttcacaa gacatcgcag atagagtact
      841 tacgataatt ttttatttta tacaacagat gcccacgaaa agaattattt tcaatagaca
      901 cctgcattac acaaa
//
LOCUS       DAZSNO010000422          908 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZSNO010000422 DAZSNO010000000
VERSION     DAZSNO010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.430"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            487..908
                     /gene="ospC4"
                     /locus_tag="ACHWN2_004523"
                     /pseudo
     CDS             487..908
                     /gene="ospC4"
                     /locus_tag="ACHWN2_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
BASE COUNT          315 a          171 c          169 g          253 t
ORIGIN      
        1 atcccgtact ctctggccag ttgggccacg caggccccta gctggtaaga tttttcaata
       61 agatcaagct taaactccat agagtcatag agtaagaggc acgaatcctg cgggggcgaa
      121 gagaatctga cattgataga gtccattaac gttataagag gactctatca tcgccaatca
      181 tcagacattg aaatagacga cactgagatg acgcttacaa ttcttttggt ttctgtaata
      241 agtaatctga catcccccgt gcccagaaga ggagagggat atcaagccca agggttaata
      301 attgatacta cggttaaaat tgccttcagt aaaggaaacg tgattaaatt caggggattt
      361 cagagtgaaa accatacaac atattaaaat ctgttcattt agctataata catacaagaa
      421 agtgtccagt tagataatgt tatctaaata accacagata aaaacgcaca taattggggg
      481 ggggggatga aaatacctga agcagtaaat catattaatg ttcagaataa tattgatctt
      541 gttgatggaa aaacgaatcc gaataaagca acaaaagcat tacaaaaaat atattacgcg
      601 taacaaactc atcttcttct ggcataagtg aaaaacattt agatcactgt gctaatactg
      661 tgaaaaactt tttaaggaag tctatagcag cgcaaagtta tagcaaaatg ttttctcaag
      721 ggaccagttt caaatcacta aatctttctc ttgaagcgcc atctggagca cggtcatcat
      781 ttcggtcact tgagcatctc gataaggtgt ccaggcacta tatctctgag ataatacaaa
      841 aagttcatcc tctttcttca gacgaacgtc acttactttc tattattatt aattctaatt
      901 ttaacttc
//
LOCUS       DAZSNO010000423          905 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZSNO010000423 DAZSNO010000000
VERSION     DAZSNO010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.431"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(208..609)
                     /locus_tag="ACHWN2_004524"
                     /pseudo
     CDS             complement(208..609)
                     /locus_tag="ACHWN2_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          228 a          206 c          194 g          277 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt
       61 tattttttac attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg
      121 taagcgatga gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac
      181 ccacgatatt cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc
      241 ccttggtgag agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc
      301 cactgcaagg ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt
      361 catcgttttc atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt
      421 gcagggctcc agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga
      481 accgttatcg gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat
      541 gacatcctgc accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc
      601 acgatcgcaa tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt
      661 aacgcttacc aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta
      721 tgcgttccgt ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac
      781 atttacacag atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt
      841 gcaaatactt agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa
      901 tgtaa
//
LOCUS       DAZSNO010000424          893 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZSNO010000424 DAZSNO010000000
VERSION     DAZSNO010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.432"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            22..893
                     /locus_tag="ACHWN2_004525"
                     /pseudo
     CDS             22..893
                     /locus_tag="ACHWN2_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          273 a          135 c          189 g          296 t
ORIGIN      
        1 aatagtttat atgaggggct tatgtatcac tcttttaata tcgctgtaat tattccttgt
       61 tataatgagc agaaggcaat agcaaaagtc ataaatgatt tcaaaacgaa tattccaact
      121 gcttctatat atgtatttga taataattcg actgacagta cagcacaagt ggcagaggat
      181 gctggagcaa cagttcactc tgttcctctg aaaggcaagg ggaatgtcgt aagacgtatg
      241 ttctcagatg tagatgcgga tatttatcta atggttgatg gagatgacac ttatgatgca
      301 agttctgctc cagaaatgat taaccatctc ataaaaaatc aattggatat ggttgtagga
      361 tgtcgtcaag agaatggaga tcaaaataca tacagaaaag ggcatcgctg aggaaataaa
      421 ttgttgactg gtactgttca gactatcttt aaagggaatt ttactgatat gttgtcgggg
      481 tatcgtgtat tttccagacg ctatgttaaa tcttttccat gtctatcaag gggatttgaa
      541 atcgaaactg agcttacaat tcatgcttta gaattacgta tgaagtatgg tgaggttaat
      601 acaaaatatg gcgagcgatc tgaagggagt gtgagcaaac tatcaacttg gagtgatggg
      661 ttcaagattc tgaaaacaat aattaaactt tactcgcttg aacggccact atactttttt
      721 agtattatcg gcgttttact tgctgctttg tctataattt taggattgcc catcattgtt
      781 gattatattg atacaggttt ggtgagaaga tttccgactg cctttttaac tgcctccatc
      841 atgttatcgt caataatggc atttgtatgt ggcattattt tgcattcaaa tac
//
LOCUS       DAZSNO010000425          892 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZSNO010000425 DAZSNO010000000
VERSION     DAZSNO010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.433"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(110..577)
                     /locus_tag="ACHWN2_004526"
     CDS             complement(110..577)
                     /locus_tag="ACHWN2_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR8933716.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="ACHWN2_004527"
     CDS             complement(643..882)
                     /locus_tag="ACHWN2_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFR8933717.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
BASE COUNT          312 a          191 c          171 g          218 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cg
//
LOCUS       DAZSNO010000426          891 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZSNO010000426 DAZSNO010000000
VERSION     DAZSNO010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.434"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(146..>891)
                     /locus_tag="ACHWN2_004528"
     CDS             complement(146..>891)
                     /locus_tag="ACHWN2_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR8933718.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT          242 a          200 c          189 g          260 t
ORIGIN      
        1 ttaacatatt ttccaatata aaaaataaga ctctggcttc aattgtgcgc ggattttctt
       61 acaggtgtag gataatagcc ttcacaaaat aattatccgc gcaattttcg tgaatggagc
      121 gcatagacta aggtcaaaat cacaattatt catagataaa agtgacacca atgactgact
      181 ggacggttcc ggctgaaacc ttgtttcctg tttgtccata ggttgcgctt aaacccagag
      241 gtactttgct tttatttact gtccccaggg agacattttc tcccgtagcc agtatttttc
      301 cattacgcat tagagtgaca cccactcccg atgcttttgt tgcatcaggt gctgtgttag
      361 caaacacttg tcggctactg tcagtggtgg cacccgaaag ataaaatgag agcttttgtt
      421 cgcttgagca atatacgcca aggggtattt ctgcgcttcc aggaaagtcc gggagatcca
      481 cggtgacatt acgactatca acggtgcagc cgcctgttgg cataacaaca ctgttatttg
      541 aaataatatt ccacgtaaaa ttacgcggat ttccgcttcc taatgttgca attttataca
      601 tatgaatacg ggcaatgact tcccccgctt ttatgacaac tccaccggcc gccccaactg
      661 gggtaatata gagttttaac ggtaatggca tcggtgtctt atcaccaata tccaatacgt
      721 tggtatttgt cgtcaacggg aaggggtagg tcacattatt ccagtacagc gatcctttat
      781 atgactgtag ggagcctgcg aaagctgagc cttgtaccag gtttatatga tcagtgtcgt
      841 accagccgcc gtaatcattc cagcaactta tatgctgaga caagtctaca a
//
LOCUS       DAZSNO010000427          881 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZSNO010000427 DAZSNO010000000
VERSION     DAZSNO010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.435"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>881
                     /locus_tag="ACHWN2_004529"
     CDS             <1..>881
                     /locus_tag="ACHWN2_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR8933719.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVK"
BASE COUNT          275 a          169 c          226 g          211 t
ORIGIN      
        1 gtctataacc acgaaccgtt atgcatcagg ttatgccaca ctgactgaag cagtaagtgc
       61 acaggatgaa cggaacagaa aaagagataa gaatagtcac gatggctcta cgatatcctt
      121 atcccagccc cttggtaata tcgggaattt gaacttcaat accacgaggt ataattcttc
      181 ccggggcaca ggaaacacgc ggtctaccag cctgagttat agtacggtat ggagaggcat
      241 aacattctcc attaactggg caaaaaacga cttgctgaca tcgcataaat ggaaagtaga
      301 tcggaaatta agcgtgggta tttcagtgcc attatcgctg ggtgacgaaa atcaaattta
      361 tgcgtcttca cagatgagtc gttcgggaga gcagggtaat aactatcagg tttcattatc
      421 aggtcagaat agtggtggtg tatggtggga tgtagcgaca aatataacta acgctcatca
      481 gtcacaaccg aagagcacga tgaacattgt gcaagttggc aaaaatggat catatggtca
      541 gtttagtagc cattacagct ccagcgaaaa catgaaacag ctaggtgcaa atttaagcgg
      601 tgggatactt attacaaggg atggacttac atttggtcaa aatgtggatg ggacattagc
      661 ccttatagag gcacccggag caacgggggt taacgttaat ggctggccgg gactgagtac
      721 tgattttcgt gggtatgcaa tactgccagt gcagccatac cgaagagacg atgttattct
      781 ggatgaaaaa acgataggta agaattacga cttaccacaa actagtcaat tggttgtacc
      841 gacagctgga gccgttgtgc ctgcgacatt ggcagtgaag a
//
LOCUS       DAZSNO010000428          879 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZSNO010000428 DAZSNO010000000
VERSION     DAZSNO010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.436"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..514
                     /gene="virA"
                     /locus_tag="ACHWN2_004530"
     CDS             <1..514
                     /gene="virA"
                     /locus_tag="ACHWN2_004530"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR8933720.1"
                     /translation="LQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISAL
                     TSAFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDT
                     AISASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQ
                     VSFAIISPDV"
     gene            693..>879
                     /locus_tag="ACHWN2_004531"
     CDS             693..>879
                     /locus_tag="ACHWN2_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8933721.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT          280 a          200 c          165 g          234 t
ORIGIN      
        1 gcttcaaatg ctaagttaca accaagtaaa taaaataatc aattcaaact tcccccaaca
       61 agacctatgt tttcaaacag aaaaactttt gtttacttca ctctttcaag accctgcatt
      121 tatatctgca ctaacatctg cattctggca atctcttcac atcacgtctt cctctgtgga
      181 acacatatat gcccaaatta tgtccgaaaa catagaaaac aggcttaatt tcatgcctga
      241 acaacgagtt attaacaatt gtggacatat tataaaaatc aacgcagttg tccctaaaaa
      301 cgacactgca atctctgcct ctggggggag ggcttacgaa gttagctcat caatattacc
      361 atcgcacata acatgtaatg gtgttggtat taataaaata gaaacaagct atctcgttca
      421 tgctggaaca cttccaagca gtgaagggct tcgcaatgct atccctccag aaagtcgtca
      481 agtatcattt gccatcatat ctcctgatgt ttaagccaat attatattgg ctgccagact
      541 gtaaaaaaac gttcttcaga acagctctat aaactattaa accaaagcta aaaggatggg
      601 gaccaataga atctcagccc atccttttta tatcattatg aaccgccccg gaaatcctgg
      661 agactaaact ccctgagaaa gaggtaaaca ggatgactaa aaatacacgt ttttcccctg
      721 aagtccgtca acgggcagtt cgtatggttc tggaaagtca gggcgaatat gactcacaat
      781 gggcagcgat ttgttccatt gccccaaaga ttggctgtat actggaaact ctgcgtgtct
      841 gggtacgcca gcatgagcgg gataccgggg gcggtgagg
//
LOCUS       DAZSNO010000429          878 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZSNO010000429 DAZSNO010000000
VERSION     DAZSNO010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.437"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            84..845
                     /locus_tag="ACHWN2_004532"
     CDS             84..845
                     /locus_tag="ACHWN2_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR8933722.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
BASE COUNT          315 a          162 c          159 g          242 t
ORIGIN      
        1 aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca tgaaattatc
       61 aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta cttaagatga
      121 ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat gactggtcag
      181 ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag tcttttctgc
      241 cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc tcacaaaata
      301 attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta aggctaaatc
      361 actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt atgaccgggt
      421 gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat ataatgaaaa
      481 cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt tctctggcat
      541 cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa atagaaaacg
      601 ctatcaatga tgcttttaac agcaagccta aaaaaataag atttttaaca gatatactct
      661 ctcctcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt aatgagattt
      721 cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt gctatcaata
      781 agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta attcagttca
      841 actgatgtaa aaatcaccct cgcttaataa ataatata
//
LOCUS       DAZSNO010000430          873 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZSNO010000430 DAZSNO010000000
VERSION     DAZSNO010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.438"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>873
                     /locus_tag="ACHWN2_004533"
     CDS             <1..>873
                     /locus_tag="ACHWN2_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR8933723.1"
                     /translation="NLSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVF
                     SKDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRL
                     MGRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDA
                     MKKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGS
                     ENGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVT
                     LAYRPLNDTQRKIV"
BASE COUNT          265 a          153 c          209 g          246 t
ORIGIN      
        1 taatttaagt tcagcagcag ttaaaacagc aggtgtactt atcagctttc aggctgtact
       61 atccgcttat cagcgagtaa tggaagttgg ccttcaacgt gcaagtgctg aacgttcaat
      121 tgatttcgta ttttctaaag atgcaaatca agtaaaagat ttcacaaagt cattagctca
      181 aacaacgggt ttagatatcg ctgaactgca atcacagttt gctggtttcg gtgcttcagc
      241 tagagagtct ctaggcattc aaggttctga agaacttttt agaaatatga ttggctattc
      301 tcgtctgatg gggcgaagtg aagaagagat taagcgagca ctgacagcat tatcacagat
      361 ggctggtaag ggtcagatta tggctgaaga gcttaagggg caactagctg aagctgttcc
      421 tggtatggtt caggtattcg ctaaagcaac cggaaagact gaacaggaat tatttgatgc
      481 tatgaagaag ggggctttaa aatctgccga tactcttcag aaggtcactc aggaactgaa
      541 taaacaaatc accgctaaag gtggctggaa agctatttca gaaagtacac aggcacaact
      601 agggaacttg aaaaactctt ggaataccac gcttgatagt atcttcagag gttctgaaaa
      661 tggtctgcaa gattttacaa gaagcttaac caatctttta aacgctcttg gtggaagtgg
      721 taagagtctt ggtgaatctc ttggtagtct catgacttct atgtcacatg gtgtcgattc
      781 attgaccgat attagttaca gggtaagggc gttctttgat gaggtgacat tagcttaccg
      841 tccactaaat gacactcaga ggaaaattgt tga
//
LOCUS       DAZSNO010000431          872 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZSNO010000431 DAZSNO010000000
VERSION     DAZSNO010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.439"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..158)
                     /locus_tag="ACHWN2_004534"
     CDS             complement(<1..158)
                     /locus_tag="ACHWN2_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR8933724.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACHWN2_004535"
     CDS             complement(308..469)
                     /locus_tag="ACHWN2_004535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933725.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACHWN2_004536"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACHWN2_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
BASE COUNT          254 a          237 c          158 g          223 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat ac
//
LOCUS       DAZSNO010000432          862 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZSNO010000432 DAZSNO010000000
VERSION     DAZSNO010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.440"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..85
                     /locus_tag="ACHWN2_004537"
     CDS             <1..85
                     /locus_tag="ACHWN2_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933726.1"
                     /translation="LIALARRRCDVLFAMMRDGTFYTPQGS"
     gene            112..459
                     /locus_tag="ACHWN2_004538"
                     /pseudo
     CDS             112..459
                     /locus_tag="ACHWN2_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
     gene            456..551
                     /locus_tag="ACHWN2_004539"
                     /pseudo
     CDS             456..551
                     /locus_tag="ACHWN2_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          242 a          211 c          194 g          215 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacgggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca ctcccccgct
      121 aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat
      181 atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg
      241 cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa
      301 tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt
      361 agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc
      421 cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat
      481 ggtcacatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact
      541 ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt
      601 ctttagaata atcaaagcgt tggattgcca tgtcatatga aaatcggcaa agttcatatg
      661 taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca
      721 tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa
      781 tactgtataa aaacacagtg cagagggatt tttatgcgtt ttgaaatctg catagccaaa
      841 gagaaaatga ctaaaatgcc aa
//
LOCUS       DAZSNO010000433          851 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZSNO010000433 DAZSNO010000000
VERSION     DAZSNO010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.441"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            44..>851
                     /locus_tag="ACHWN2_004540"
     CDS             44..>851
                     /locus_tag="ACHWN2_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8933727.1"
                     /translation="MANLYDLKKLDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHP"
BASE COUNT          278 a          183 c          154 g          236 t
ORIGIN      
        1 aagtgaatat accatggaag aaaaatataa accggagtag tgtatggcca atctctacga
       61 cttgaaaaag ctcgacttaa atcttcttgt catatttgag tgtatttacc agcatttaag
      121 cattagcaaa gctgctgaat cactatatat caccccatca gccgtgagcc agtcgttaca
      181 acgcttacgt gcgcaattta atgacccttt attcattcgc tcaggaaaag gtatcgcgcc
      241 gacgacaaca gggttgaatc tgcatcacca tcttgagaaa aacctcaaag gccttgaaca
      301 aacaattaat atagtcaata agtccgaact aaagaaaaac tttattatct acggccctca
      361 gctcatttct tgctctaata atagtatgct gatccgctgc ctacggcaag atgcctctgt
      421 cgagattgag tgccatgata tcctcatgtc agcagagaat gctgaagaac tgttggttca
      481 ccgcaaagcc gatctggtga tcacccaaat gccagtgatc agccgttccg tcatttgcat
      541 gccattgcat acaattcgca atactctaat ttgtagtaat aagcatccgc gaattactga
      601 caatagcact tatgaacaaa ttatggcaga agagttcacc caattaatag ccaaatcagc
      661 aggagttgat gatattcaaa tggaaataga cgagaagttt atgaatcgca aaatttcatt
      721 tcgtggttcc tctttgctga caataattaa tagcattgca gttacagatt tactgggaat
      781 agtcccctat gaactttata actaccaccg tgactttctt aatttaaaag agataaaact
      841 agaacatccg t
//
LOCUS       DAZSNO010000434          851 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZSNO010000434 DAZSNO010000000
VERSION     DAZSNO010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.442"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            59..556
                     /locus_tag="ACHWN2_004541"
                     /pseudo
     CDS             59..556
                     /locus_tag="ACHWN2_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            597..788
                     /locus_tag="ACHWN2_004542"
                     /pseudo
     CDS             597..788
                     /locus_tag="ACHWN2_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          219 a          210 c          250 g          172 t
ORIGIN      
        1 gcgccagccc cgtcccgccc gatagagcgc ggcatagcgg gccccggatt acttgcgtgc
       61 gacggcagaa gaagccctga agcgaatcag tgaactgtac gccatcgagg atgaaatacg
      121 gggattacca gagtcagagt gtcttgcagt caggcagcag cgaagcaaag cgttactgac
      181 gtcgctgcat gaatggatgg tggagaagaa tggcacgctg tcgaaaaaat ccagactggg
      241 cgaagcattc agctatgtac tgaatcagtg ggacgccctc tgttattaca gtgatgacgg
      301 tctggcggag gcggacaata acacagcgga aagagcgctt cgtgcagtct gtctcggaaa
      361 gaaaaattac gtgttcttcg gtagcgatca cggcggcgag cgtggtgcac tgctgtacgg
      421 gctgatcggc acctgccgtc tgaacggtat cgatccggaa gcgtatctgc gccatattct
      481 gagcgtactg ccggaatggc cttccaaccg agttgacgac ctcctgccat ggaaggtggt
      541 gctaccatca ggataatcgt caatacggtg ttcggtgaac gtttacttgg agcatcatgc
      601 ttcatgaaga aaaactggca cgtcatcaac gtaaacaggc gatgtatacc cgaatggcag
      661 ccttcccggc ggtgaaaatg ttcgaagagt atgacttcac gttcgccacc ggagcaccgc
      721 agaagcagct ccagtcgtta cgctcactca gcttcataga gcgcaatgaa aacatagtct
      781 tactggggac atctgacatc acaaatcccc gggtggggat ttgtgtataa gagacagccc
      841 atggaggaag g
//
LOCUS       DAZSNO010000435          845 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZSNO010000435 DAZSNO010000000
VERSION     DAZSNO010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.443"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..85
                     /locus_tag="ACHWN2_004543"
     CDS             <1..85
                     /locus_tag="ACHWN2_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933728.1"
                     /translation="ETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(139..>845)
                     /locus_tag="ACHWN2_004544"
     CDS             complement(139..>845)
                     /locus_tag="ACHWN2_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005078762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933729.1"
                     /translation="CEGNGTQGDSSTGGAQLAINGLNGSCALVLNNPYFEANAGGADL
                     AIDNTGTRPVTVVINGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTTFQSAGDYQ
                     PSAERPYWITGANCEVVDIGCVFTETTSKVTSASALSVTRSGKINANGSIDVATGVSS
                     VNVVSTGVYDVSFSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLS
                     GAGISSSFAFSITRLI"
BASE COUNT          257 a          214 c          157 g          217 t
ORIGIN      
        1 agagacgcca ttcatcaaat taacaaaaca aaccacaact ttagtaaaag aattaatacc
       61 cacattaaca aacaacaatc aataaacaat aaacataagc cgccctacat atatgtccaa
      121 atggggcggc gaatgtattt atattagtct ggtaatgcta aatgcaaacg aactggagat
      181 tcctgccccg ctaagcgtat tgcgcagggt caccctaaat gtatctacgc caatataagt
      241 aacatcacaa ctaactgaat caggcgcaga tataggtgtt atctgcacaa tataaccgct
      301 agcaactgct gcgagcggat gagaaaagct aacatcatac acacctgtag aaaccacgtt
      361 aaccgatgaa acaccagtag caacgtcgat actaccattg gcgttaattt ttcccgagcg
      421 tgtgactgac aatgcagaag ccgatgttac cttactggtt gtttctgtaa aaacacaccc
      481 aatgtcaact acctcgcaat tagctcctgt tatccagtat ggtcgttctg cacttggctg
      541 ataatcccct gcgctctgga acgttgtgcc gttgagtatg actgtaactt ttcccccacc
      601 tgagcttgtt acttgaatat tcgtgtgcgt gtatcttgca ctgctcacgc gatgaaaatt
      661 tccaccgttt ataaccacag ttacagggcg cgtcccagta ttatctatag caaggtctgc
      721 ccccccggca ttagcctcaa agtatgggtt gtttaatacc aaagcgcacg aaccgttaag
      781 gccatttata gctaattgcg caccacctgt actggaatcc ccctgagtac catttccttc
      841 acaag
//
LOCUS       DAZSNO010000436          831 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZSNO010000436 DAZSNO010000000
VERSION     DAZSNO010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.444"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..326
                     /locus_tag="ACHWN2_004545"
     CDS             <1..326
                     /locus_tag="ACHWN2_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR8933730.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="ACHWN2_004546"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="ACHWN2_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR8933731.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          154 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca agttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgctccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DAZSNO010000437          826 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZSNO010000437 DAZSNO010000000
VERSION     DAZSNO010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.445"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..772
                     /gene="ospB"
                     /locus_tag="ACHWN2_004547"
     CDS             <1..772
                     /gene="ospB"
                     /locus_tag="ACHWN2_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR8933732.1"
                     /translation="KNSSCTHPKAALVFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLY
                     DYVFKNEKSKNDFLKMAESWLPQSEPIVINNDDDALNAAAYFSVKKAKIKTVNDTDFK
                     EYNKVYILGHGSPGSHQLGLGSELIDVQTIISRMKDCGILNVKDIRFTSCGSADKVAP
                     KNFNNAPAESLSCILNSLPFFKEKESLLEQIKKHLENDESLSDGLKISGYHGYGVHYG
                     QELFPYSHYRSTSIPADPEHTVKRSSQKKTFIINKELD"
BASE COUNT          292 a          123 c          144 g          267 t
ORIGIN      
        1 aaaaaattca tcatgtactc acccaaaagc agcattggtt tttttaggag agaaaggttt
       61 ttgtgatagc aatgatgttc tatctattat gggacaacaa ataccaagag tatttaagaa
      121 caagatgtta tatgattatg tttttaaaaa tgaaaaaagt aaaaatgatt ttctaaaaat
      181 ggctgaatca tggctaccac agagtgaacc aatagtaata aataatgatg atgacgcatt
      241 gaatgctgct gcttattttt ctgtaaaaaa agcgaaaata aaaacagtaa acgatactga
      301 ttttaaagag tataataagg tttatattct tgggcacggt agtcctggtt ctcatcaatt
      361 aggccttggt tcggaactta ttgatgtaca aacaatcatt tcaagaatga aagactgtgg
      421 tattctaaat gtgaaagata tccgttttac ttcatgcggc tccgctgata aagtggctcc
      481 taaaaatttt aacaatgccc ctgctgaaag tctttcttgt atccttaact ctctgccttt
      541 ttttaaggaa aaagaatctt tgctagagca gataaaaaaa caccttgaaa acgatgagtc
      601 attgagtgat ggtctaaaaa tatccggcta tcatggatat ggagttcact atggtcaaga
      661 gctttttccc tactcacatt atcgttcaac ttcaattcct gctgatccgg agcatacagt
      721 aaaaagaagc tctcagaaaa agacttttat tattaataaa gaactggatt agtataaaat
      781 ttttaaccta tagttaattg tttgcattta agatgaagtt atttta
//
LOCUS       DAZSNO010000438          823 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZSNO010000438 DAZSNO010000000
VERSION     DAZSNO010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.446"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..176
                     /gene="ipgE"
                     /locus_tag="ACHWN2_004548"
     CDS             <1..176
                     /gene="ipgE"
                     /locus_tag="ACHWN2_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR8933733.1"
                     /translation="NYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYYISRVR
                     WLKDEFARRMKGY"
     gene            176..634
                     /gene="ipgF"
                     /locus_tag="ACHWN2_004549"
     CDS             176..634
                     /gene="ipgF"
                     /locus_tag="ACHWN2_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR8933734.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            643..>823
                     /locus_tag="ACHWN2_004550"
     CDS             643..>823
                     /locus_tag="ACHWN2_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFR8933735.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTD"
BASE COUNT          282 a          107 c          194 g          240 t
ORIGIN      
        1 taaattactc agaaaagata tgcttagcta ctgatgatga aggcggaaat ctaattgcgc
       61 gattagatct taccgggata aatgaattcg aggatgtata tgttaataca gaatattata
      121 tttcgcgtgt gcgttggctg aaggatgaat ttgcgcgaag aatgaagggg tattaatgtc
      181 ccgtttcgta tttattttgc tgtgttttat cccatattta gggagagctg attgttggga
      241 taaggctggt gaaaggtaca atattccatc aagtttgtta aaagcgattg cggaaaaaga
      301 atccggattt aataaatctg cagtcaatgt taataacaat ggaagtaaag attatggtat
      361 aatgcaaata aatgactttc attctaaaag acttagagaa atgggatatt ctgaggaaat
      421 gctaattagt catccatgtc tttctgtaca ttatgcagca aagttattaa atgagtttat
      481 gatgatgtat ggaagagggt gggaggctgt tggagcctat aatgcaggaa cgtctccgaa
      541 aaagaaaaaa gaaagattaa agtacgcaga ggatatctat agacgctatt tgcggatagc
      601 agcagagagc aagcagaata atcgaaggat ataagaggat ttatgtctga ggcaaagaac
      661 tcaaatcttg caccattcag gttattagtg aaactcacaa acggtgtcgg ggatgagttt
      721 ccgttatatt atgggaataa cttaattgtt ctcggacgga cgatagaaac acttgagttt
      781 ggaaacgaca atttcccaga aaatattatt cctgtaacag ata
//
LOCUS       DAZSNO010000439          811 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZSNO010000439 DAZSNO010000000
VERSION     DAZSNO010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.447"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..794
                     /locus_tag="ACHWN2_004551"
     CDS             <1..794
                     /locus_tag="ACHWN2_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933736.1"
                     /translation="IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRF
                     VNSQAHIIMCGRAGYEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNI
                     ETGQVWRTARILKDRSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELF
                     ANDGTPTWLKEKRAKEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIK
                     GMDWPTIKAGRNALWIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          264 a          185 c          194 g          168 t
ORIGIN      
        1 tcatcgactc catcagtcac ttctggaccg gattatgcga cgagtatgcc agacgccgaa
       61 accgtaaacg aggactggaa ttttcagact gggcgtggct caaacaggag tggcgacgct
      121 ttaccgatcg atttgttaac agtcaggcgc acattattat gtgcgggcgt gcaggatatg
      181 agtatgactt tttcgaaggt gatgatggca aacgccagtt ggcaaaaacc ggtataaaaa
      241 tgaaagcaga aacagaaacc ggttatgaac cctccatccg gatccagatg gagaagcaaa
      301 tgaacataga aaccggtcag gtgtggcgca ccgccagaat actaaaagat cgctccaccc
      361 gtattgatgg acaggtattt tcaaacccaa catttaaaaa ctttcttcct catatcgagt
      421 ctcttaacct gggaggtgaa caccccggca tcgatacctc acgggataac agtgaattat
      481 ttgccaacga cggtacaccg acatggctaa aagaaaagcg tgcaaaagaa atcgcacttg
      541 atgaaattat cgagttgctc aacaagcacc atgggggcac cagtaacgac gccaaacgcg
      601 caaaagctga tttgctggaa aaaacattca cctcccgctc atgggaaaga atcaaaggta
      661 tggactggcc gacaatcaaa gctggtcgta atgcactatg gattgaactg gaaggcgttg
      721 aatatgcatt cccggatcca caaacacaaa acgaaacgca acaagcagga tacgatgaga
      781 atattccggt gtaaaaccaa gtgcattttg g
//
LOCUS       DAZSNO010000440          806 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZSNO010000440 DAZSNO010000000
VERSION     DAZSNO010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.449"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          246 a          152 c          166 g          242 t
ORIGIN      
        1 ttacccagag tcataaaata gagtgtctgt tgatctcatt ttcatattcc tttcagactg
       61 aagtgcattt tcatcacccg atgtgaaaga actgaagtga tgtttgccat agattacgag
      121 atgcccagtc tcattgacta tcgtggtgca tctttatacc tgttatacca gatcaaaaat
      181 cacgcaatcc atacaacaaa accagatttg caattcgcgt cacaaaatat gtcgatcttt
      241 ttctaagaag aagatgccat gtgaagccag acgaacactt gcggtggtct tcaaaaacta
      301 aagattttct tttaactata cgttttataa ataatttatt tagaatctaa acaaagaaag
      361 ctttgcaagc taactcagtg agagtggtga aaataagggt ttactcgcca tcaggctgag
      421 cataattctc atcatgaaat atgtttcttg gtttgtggct tgtaactggt cactcctgat
      481 gtcgatctgg agaggcttgt tgatgttggt gttttcagga tgatgtttca cttagtttgt
      541 ttgccgtatc gcccggcgaa tagctgtgat tgaggaaggc ttaagtcgta gtgaccaaag
      601 ctatatttac caacggatgt agatgaaaaa gcgtcgcctg cgttccccca tatctccagg
      661 ataaaaagga atgtaacaat ctcatggcct aagctgacga accaggcagg aataatcgct
      721 agggatctaa gaattagcat gataatagcc actaagaaat tactgcgttc catgaaatag
      781 ccattttgtg gcaaatgcag ttaact
//
LOCUS       DAZSNO010000441          804 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZSNO010000441 DAZSNO010000000
VERSION     DAZSNO010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.450"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(22..693)
                     /gene="queE"
                     /locus_tag="ACHWN2_004552"
     CDS             complement(22..693)
                     /gene="queE"
                     /locus_tag="ACHWN2_004552"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR8933737.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          203 a          210 c          176 g          215 t
ORIGIN      
        1 ataacgctta taaatgtgta atcaggcaat atttaaatat ttatgtgttt gcatcgacaa
       61 acgccaatta cgcgcaatac aggtttcaat gcacaatcgt gtggcatcat ccttttggct
      121 aatcggctgc agtgcaatga ctcgcggttt atcatcggtc agcgttgcca gtagttcatc
      181 cagtgcttca atatcgcgta cgcgccccac tggatgcttg atttcgttgg ctcgctccag
      241 tgcctgtgac aacacttcat agccgccgcg catgttcagc tttggcgata cggtaaccca
      301 ggtattcggt gtgcagcgta cttcatgagt tccactggtt tcgatctggc agctaaaacc
      361 gttcttttcg agcaggtcag tcagtggcag caaatcatga atgcaaggct caccacccgt
      421 aatcaccaca tgccgcgcgg tatatccctg acgaccaatg acagccagca agtcttcact
      481 gctcgccgcc ccccacttat cactctcttt ggtcttcgcc agaatgctga aaagggagac
      541 ttcccgatcc tcaagctttt cccaggtgtg tttggtgtcg caccaggcac agccaaccgg
      601 gcatccctgt aaacgaataa aaatggcggg aacgccggta aagtaaccct caccttgcag
      661 ggtctggaac atctcgttaa tcgggtactg catagcattc tctgtgaagt ggataattgt
      721 taattattgc agatcctgcc gcaacaatca tgtcttatta acattctgtt acatgcagat
      781 ttaaagaaaa atacgaaaca tatc
//
LOCUS       DAZSNO010000442          800 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZSNO010000442 DAZSNO010000000
VERSION     DAZSNO010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.451"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(15..611)
                     /gene="fimE"
                     /locus_tag="ACHWN2_004553"
     CDS             complement(15..611)
                     /gene="fimE"
                     /locus_tag="ACHWN2_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFR8933738.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          246 a          192 c          150 g          212 t
ORIGIN      
        1 atcaataagt taaatcaaac ctcttctctt tttaattttt cgtttatgag attatttctt
       61 tcccataatc cggcaaaacg agcagcatta ctggcggtat aacgcacagt atggcgaata
      121 tttcgatgcc cgagataatc ctgaattaaa cgggtatctg caccacgctc cgccagttca
      181 taaccgcaag catgccttaa catatgagga tgagtctgcg ttacggttcc agcttcaata
      241 ccggcatcgc gaataatgcg ataggcctgc tggcgagaaa gccgactccc gcggcgagaa
      301 ataaatatag cgtcggtccg gtcagcgcct ttccagttag cacgttcctg ggtccagcgt
      361 tccacggctt cacgctcatc aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt
      421 cggcgaatat ttattcgacc ttcattaagg tcaaggtcct gataatgcag atcaagcagt
      481 tcactaatac gcatcccatg ccgatatgcc aacagaataa gacaataatc tctggctccc
      541 gttgccccgt aacaaaccgc ctgcatcatg gcctgaactt ctttaccggt aagataacga
      601 cgtttactca caatagtagt actcctaact gagatatatt tgaatgaata cctataggaa
      661 acctcaatcg gtcaaaatta gcccaataga taaaaagata accacacacg aagttatgtg
      721 gctatcagtc aattacatta aacatcatac aaaaataaaa tatcactgat gtgacaacat
      781 ctattttcat tgatttgatt
//
LOCUS       DAZSNO010000443          789 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZSNO010000443 DAZSNO010000000
VERSION     DAZSNO010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.452"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>789
                     /locus_tag="ACHWN2_004554"
     CDS             <1..>789
                     /locus_tag="ACHWN2_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR8933739.1"
                     /translation="CERFFFWQLRSFKLDVRGYARHFKVLNSCLFALPPIAEQERIVE
                     KVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQNTAELAENWARISEHFDTLF
                     TTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPL
                     PPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYLNLYQVAPDIIEKGTGRVISK
                     RTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYNGLCSAD"
BASE COUNT          260 a          154 c          174 g          201 t
ORIGIN      
        1 ttgtgagcgt ttcttttttt ggcagttacg atcatttaaa ttagatgtaa gaggatacgc
       61 gagacatttt aaagtattaa atagctgctt atttgcatta ccccccattg cagaacaaga
      121 gcgaatagtt gaaaaagtta gctctttgat gtccctctgc gaccaactag aacagcaatc
      181 cctgaccagt ctggacgcac atcagcaact ggttgaaacc ctgttgggaa cgcttacaga
      241 cagccaaaac accgcggaac tggctgaaaa ctgggcgcgt attagcgagc atttcgacac
      301 cctatttacc accgaagcca gcgtggatgc gttaaaacag accattctgc aactggccgt
      361 aatgggcaaa cttgtgccgc aggatccgaa tgacgaaccc gcctctgaac tgctcaaacg
      421 tattgcgcag gaaaaagctc aattggtgaa agaagggaaa ataaaaaaac aaaaaccact
      481 gccgccaatt agcgatgagg aaaaaccgtt tgaacttccg gaagggtggg aatggtgtcg
      541 tattggtaat atagttaata ttaaatctga attagtatca ccaaaagatt atttaaattt
      601 atatcaagta gcacctgata ttattgaaaa gggcactggg agagtgatat caaagagaac
      661 agtgaaagaa tcaggagtca aagggccaaa tagtcgcttt tataaaggac aaattgttta
      721 ttctaaaatc cgcccgtcat tatccaaagt ttttttagca gagtataatg gtctttgcag
      781 tgctgatat
//
LOCUS       DAZSNO010000444          784 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZSNO010000444 DAZSNO010000000
VERSION     DAZSNO010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.453"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..513
                     /locus_tag="ACHWN2_004555"
                     /pseudo
     CDS             1..513
                     /locus_tag="ACHWN2_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            517..676
                     /locus_tag="ACHWN2_004556"
                     /pseudo
     CDS             517..676
                     /locus_tag="ACHWN2_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          200 a          193 c          218 g          173 t
ORIGIN      
        1 ccgctgtatg acatcctgcg ccagtatgtg ctgatgcccg gtaaagtcca tgccgatgat
       61 atccccgttc cggtccagga gccgggcagc ggtaaaacaa ggactgcccg gctgtgggtc
      121 tacgttcgtg atgaccgtaa cgccggttcg gaaatgcctc cggcggtctg gttcgcgtac
      181 tcaccggacc ggaaaggtat ccatccacag aatcatctgg ccggttacag cggagtgctt
      241 caggccgatg cttacggtgg ttaccgggcg ttgtacgaat ccggcagaat aacggaagcc
      301 gcatgtatgg ctcatgtccg gagaaaaatc cacgatgtgc atgcaagagt gccaaccgac
      361 atcaccacgg aagccctgca gcgtatcggt gaactgtatg ccatcgaggc agaagtccgg
      421 ggatgttcag cagaacagcg tctggcggca agaaaagcca gagccgcgcc actgatgcag
      481 tcactgtatg actggataca gcaacagatg aaaattcatt cgctgaaaat ggaatgtctc
      541 cacggagaac actattatcc atcgggaaat agtgcgggca acagtgttta gttatatcga
      601 gtgcgattac aatcgctggc gacggcaccg ttggtgtggt ggtctcagtc cggaacaatt
      661 tgaaaaccag agcctcactt acgactgtgt ccatattatg tgggtaggat caataaagac
      721 gttttatatg tgtctgcttg cagccagaaa atttataccc tgcaggtatc ataaacatgc
      781 ttga
//
LOCUS       DAZSNO010000445          784 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZSNO010000445 DAZSNO010000000
VERSION     DAZSNO010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.454"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..668)
                     /locus_tag="ACHWN2_004557"
     CDS             complement(<1..668)
                     /locus_tag="ACHWN2_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFR8933740.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(658..>784)
                     /locus_tag="ACHWN2_004558"
     CDS             complement(658..>784)
                     /locus_tag="ACHWN2_004558"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFR8933741.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          252 a          203 c          177 g          152 t
ORIGIN      
        1 tatggcagtg ctattttgat tcgacgttga tcaagtttct gataattcca acgacggtcg
       61 acgggcacca tccagtcaat caacatacct accgccagcc ctgtgatgac atcaataaaa
      121 tgatgctgcc atgtcgtcag cgtcgaaatg gcgatgagta aaaaccatcc gccacagact
      181 ttacgccacc tcacagccag atgctgacga aagtgacgcc aaagtagcca gcagagaata
      241 atatgcagcg aaggagactg gttataaggc agatcaaaca gttcaagttg cgaaaatagc
      301 catcctgtca ccccactcac ttcaggacgg ataaaactaa acttcagcgg gtagagaaaa
      361 aaaccgcagc aggccattac cgttgccaga ataagccggt ggacaagtcg gcgttgttcg
      421 aatgtcgagc tacaaacgaa cagcgaaaat ccatataaaa gatccagact ccagtaagga
      481 acaatagtcc agggaagaaa agggatcgcc gtttcccaac cgaatacctg actggggata
      541 tcatggctgt taaggtcctg aaccgcggtg aactgattaa gagatccata ggtgaagaag
      601 aaaaacgggg ccagcaacaa taaccagcca gcgccttgta gcaatacgtt ccgacgttca
      661 gttatcacga cgcaccgcca tcgaaaccgt aaaaatgccc cacacatcaa tccgttgagt
      721 gcatttatca aatccggcat tacgcacgag cgaatccatc tccccttgcg aacgcacgcg
      781 catt
//
LOCUS       DAZSNO010000446          772 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZSNO010000446 DAZSNO010000000
VERSION     DAZSNO010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.455"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(72..677)
                     /locus_tag="ACHWN2_004559"
     CDS             complement(72..677)
                     /locus_tag="ACHWN2_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR8933742.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          222 a          174 c          128 g          248 t
ORIGIN      
        1 ccatattttt gggatgtggc tataattatc tattcccgct atttagtctt caaaaaaaat
       61 tacatttaat atcatttatc ttgattaaga aatagtaaga ctaaaaaaac aagcgtcgtc
      121 agccagcaca ctgctaacac ggtaagaacg ctaataaaaa cactccactg cttcgttcta
      181 taccattcaa tatttttgtc agtatgtttt attgggagca gctcttcagt agagaattcc
      241 tggctgttat cattctcagg aatgtttgca tcagtgttta cttccctggc ctcgctatcg
      301 ttggtatagc tactactgtc ttcatcactg tcacgctcat tctcacagac atgtttctcg
      361 acctttccca gatatctgac gccttttcgc ggaatggttt taattagaga actcgacaaa
      421 cctgcgacgt ctaatgcctt tctaagcatg taaatctgcc cataataact gctgtcgctt
      481 accgaacctt tctgctcccg ccaaacctca ttgataatat gttgcttatc ggattctccc
      541 tgtagtaaaa ggagtaaaaa tcgatagccg ttttcagaca gattgattac attgcctgta
      601 ctggtggtga tacgtcgaga cgctgggtaa aacttaactc cctgccctaa agaaaaataa
      661 aaacggtcat cgctcataag taccgacccc tgttttcttc ttcacatgac tactcattac
      721 aactttgtat gaagtaagat gcaaaatcat attgtttaca ttaagttaag ca
//
LOCUS       DAZSNO010000447          761 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZSNO010000447 DAZSNO010000000
VERSION     DAZSNO010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.456"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..556
                     /locus_tag="ACHWN2_004560"
                     /pseudo
     CDS             1..556
                     /locus_tag="ACHWN2_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          197 a          196 c          176 g          192 t
ORIGIN      
        1 aatcaggtct gggtcgcaga ttcagttgtc caggccttcc gaaatcaacc gactgaaggc
       61 gctggtcgcg aaactgcagc gtatgcagtt cggtaaaagc tcagaaaaac ttcgcgcaaa
      121 aaccgaacgg tggatacagg aagcacagga gcgaatcagc gcacttcagg aggaaatggc
      181 ggaaacactg ggtgagcaat atgacccggt actgccatct tccctgcgcc agtcttcagc
      241 ccgtaaaccg ttaccagcct cacttccccg tgccccccgg gtcatccggc cggaagagga
      301 atgctgtccg gcctgtggtg gtgaactcag tccactggga tgtgatgtgt cagagcagct
      361 ggagcttatc agcagcgcct ttaaggttat cgaaaaacaa cgccctaaac tggcctgtcg
      421 ccggtgcgac catatcgtgc aggcaccggt gccttcaaaa cccattgcac gcagttatgc
      481 cggagcgggg cttctggccc atgttgttac cggaaagtat gcagaccatc tgccattata
      541 ccgccaaagt gatctactat tccacacagc tatttagctc tctttttgtt gagctcccat
      601 gattctttca tttattgtta ctaattattt tatgttatca tgatgttaga acacgctctg
      661 ctctgtgtct ctattttatc tatcatattc tgaggattaa ctacataaca aggcgcaaga
      721 tatatataca aaaacaaata ataccgttgc aataattcct t
//
LOCUS       DAZSNO010000448          757 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZSNO010000448 DAZSNO010000000
VERSION     DAZSNO010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.457"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>757)
                     /gene="ipaH7.8"
                     /locus_tag="ACHWN2_004561"
     CDS             complement(<1..>757)
                     /gene="ipaH7.8"
                     /locus_tag="ACHWN2_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HFR8933743.1"
                     /translation="NNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEERGIAF
                     NRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKELNADN
                     NRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLKFLHV
                     YYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPESFSQ
                     LDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
BASE COUNT          236 a          111 c          168 g          242 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttattta
//
LOCUS       DAZSNO010000449          752 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZSNO010000449 DAZSNO010000000
VERSION     DAZSNO010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.458"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..265)
                     /locus_tag="ACHWN2_004562"
     CDS             complement(<1..265)
                     /locus_tag="ACHWN2_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFR8933744.1"
                     /translation="MRDVQWFCPLTPVHTGTEIKPVEMPVTTITPVSDAGGLQDFIYW
                     RPDADRAGMEPVYVILNDPLDSGRFTRKQLNKNILNMQVILVFS"
     gene            540..752
                     /locus_tag="ACHWN2_004563"
                     /pseudo
     CDS             540..752
                     /locus_tag="ACHWN2_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          193 a          186 c          182 g          191 t
ORIGIN      
        1 tcgagaatac caaaattact tgcatgttta agatattttt atttagttgt ttacgcgtaa
       61 atcgcccgga atccagagga tcattaagta tcacataaac aggttccata ccagccctgt
      121 ccgcatcagg acgccagtaa ataaaatctt gtagtccacc ggcatcagag acaggagtaa
      181 tcgtcgttac cggcatttcg actggtttta tttccgtacc tgtatgtact ggtgtgagcg
      241 gacaaaacca ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg cccgctaacg
      301 cggagatacg ccccgacttc gggtaaaacc ctcgtcggga ccactccgac cgcgcacaaa
      361 agcattttca tcgccgatag cgggtatggc ttggcagggc cgggatgggt ataggtaatt
      421 tggctatcgt aaaaaaggtg gttaaaaatt acaacctgca atgtaaattg gctagttaat
      481 ttttggctat ttaaattgac ttcttaacgg cgtttactgt tccattgctc cccaaccccc
      541 ctaatcaggt ctgggtcgca gatataacgt atgttgccac ccgggaagga tggctgtatc
      601 tcgctggcgt caaagatgtt tatacgtgcg aaattgtcgg ttacgccatg ggagagcgca
      661 tgacgaaaga gttgacgggt aaagccctgt ttatggcgct caggagccag cgcccacctg
      721 ccgggctaat ccaccacacc gatcgaggtt ca
//
LOCUS       DAZSNO010000450          750 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZSNO010000450 DAZSNO010000000
VERSION     DAZSNO010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.459"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(1..217)
                     /locus_tag="ACHWN2_004564"
                     /pseudo
     CDS             complement(1..217)
                     /locus_tag="ACHWN2_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            201..459
                     /locus_tag="ACHWN2_004565"
                     /pseudo
     CDS             201..459
                     /locus_tag="ACHWN2_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(568..>750)
                     /locus_tag="ACHWN2_004566"
     CDS             complement(568..>750)
                     /locus_tag="ACHWN2_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933745.1"
                     /translation="SSIDKYITDMCGLICMRHIESTGIIFYMLDEKVLSAIAEFMNEK
                     GWRAAFCSAKNLYKCV"
BASE COUNT          208 a          190 c          144 g          208 t
ORIGIN      
        1 atatcagctt ttcctgcctg gattcggtca tccatttatc gctgtgctgc cacaggaagc
       61 gagttccctt tgcctgacga cgactttcaa caggaagatg cggatgttca ttctggcggg
      121 ttttatccac cacctcaccc ggttgtttcg ccacatgaaa gcgatcgaag gctattttct
      181 caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt gctgagccag tttagcattg
      241 ccttctacat gaccgatgcc tgagccggta taccgaactt tattgtcctc aaccagtcat
      301 gttataagca agaaatgcac tcagcggata gagcgacata atctgaacct gcggacacat
      361 cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt ctgatgatat gcactacaag
      421 atcatcggct ggtatctgac aatcaaccac caccactaaa tctgcgtcac gacctgcaac
      481 tgagtctggt tgaacaggct gaggccctgc tgaagcaacg cgaacctctc gactcttgtt
      541 ggattttacc aaatagtttt tctaaaatta aacacattta tataagttct ttgctgaaca
      601 aaacgcagcc cgccatcctt tttcattcat aaattcagca atggcagata aaactttttc
      661 atcaagcata taaaaaataa tgccagtaga ctcaatatgc ctcatacaaa taaggccgca
      721 catatccgta atatatttgt caatgctgct
//
LOCUS       DAZSNO010000451          748 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZSNO010000451 DAZSNO010000000
VERSION     DAZSNO010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.460"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            31..747
                     /locus_tag="ACHWN2_004567"
     CDS             31..747
                     /locus_tag="ACHWN2_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR8933746.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          248 a          128 c          165 g          207 t
ORIGIN      
        1 tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa tattgttgct
       61 aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg tgtagaaatc
      121 tatgctgctg ccaaacaagg tgaacccgat ccagaattaa acacatcttt aaaattcgtt
      181 attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc aattgataaa
      241 gccaaaggcg gcggagatga aacgttcgta cagggacgtt atgaaggctt tggtcctaat
      301 ggctcaatga ttattgctga gacattgact tcaaatgtta accgtacgat tgctaacgtt
      361 cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt cagctatatg
      421 tttgacaata cgggtgtgat tgtatttaaa ggaacagacc ctgaccatat ttttgaaatt
      481 ttgcttgaag ctgaagttga tgttcgtgat gtaactgaag aagaaggtaa tattgttatt
      541 tatactgaac ctgctgacct tcataaagga atcgcggcgc taaaagcagc tggaatcact
      601 gagttctcaa caacagaatt agaaatgatt gctcaatctg aagttgagct ttccccagaa
      661 gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgacga tgtacaaaaa
      721 gtttatcata acgtcgcaaa tctctaat
//
LOCUS       DAZSNO010000452          746 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZSNO010000452 DAZSNO010000000
VERSION     DAZSNO010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.461"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..746
                     /locus_tag="ACHWN2_004568"
                     /pseudo
     CDS             1..746
                     /locus_tag="ACHWN2_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          171 a          203 c          226 g          146 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc agaaaatctg ttggagcagg
      481 atttttacgc cagtggcccg aaccagaagt gggcaggaga catcacgtac ttacgtacag
      541 atgaaggctg gctgtatctg gcagtggtca ttgacctgtg gtcacgtgcc gttattggct
      601 ggtcaatgtc gccacgcatg acggcgcaac tggcctgcga tgccctgcag atggcgctgt
      661 ggcggcgtaa gaggccccgg aacgttatcg ttcacacgga ccgtggaggc cagtactgtt
      721 cagcagatta tcaggcgcaa ctgaag
//
LOCUS       DAZSNO010000453          746 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZSNO010000453 DAZSNO010000000
VERSION     DAZSNO010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.462"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            44..>746
                     /locus_tag="ACHWN2_004569"
     CDS             44..>746
                     /locus_tag="ACHWN2_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR8933747.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWD"
BASE COUNT          240 a          144 c          149 g          213 t
ORIGIN      
        1 ttcttaatac tttgttcatt caataaatga gttgagagaa tatatgaata aaatatactc
       61 ccttaaatat agtgctgcca ctggcggact cattgctgtt tctgaattag cgaaaagagt
      121 ttctggtaaa acaaaccgaa aacttgtagc aacaatgttg tctctggctg ttgcaggtac
      181 agtaaatgca gcaaatattg atatatcaaa tgtatgggcg agagactatc ttgatcttgc
      241 acaaaataaa ggtattttcc agcccggagc aacagacgta acaatcactt taaaaaacgg
      301 agataaattc tctttccata atctctcaat tccggatttt tctggtgcag cagcgagtgg
      361 cgcagctacc gcaataggag gttcttatag tgttactgtt gcacataaca aaaagaaccc
      421 tcaggccgca gaaactcagg tttacgctca gtcttcttac aaggttgttg acagaagaaa
      481 ttccaatgat tttgagattc agaggttaaa taaatttgtt gtggaaacag taggtgccac
      541 cccggcagag accaacccta caacatattc tgatgcatta gaacgctacg gtatagtcac
      601 ttctgacggt tcaaaaaaaa tcataggttt tcgtgctggc tctggaggaa catcatttat
      661 taatggtgaa tccaaaatct caacaaattc agcatatagc catgacctgt taagtgctag
      721 tctatttgag gtcacccaat gggact
//
LOCUS       DAZSNO010000454          731 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZSNO010000454 DAZSNO010000000
VERSION     DAZSNO010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.463"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..590)
                     /locus_tag="ACHWN2_004570"
     CDS             complement(<1..590)
                     /locus_tag="ACHWN2_004570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR8933748.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCS"
BASE COUNT          228 a          159 c          128 g          216 t
ORIGIN      
        1 ttactgcatt ttcctgtcat ttctttcaaa tattccctcc cgctcaacaa gtcctgccga
       61 agatgtttac actgttcttc tcgtgaactg aactttttat cccagcgtgt atgagtatgc
      121 gtatgaacat gaaattcgac cagaccagat tgcaacattt cgtttacttc agaccagcga
      181 agcataacat tatcagcatt ccctgtcgct atttgatgtt cgcaatcgcg atgagaatat
      241 tcctttccag gagagtgacg aaccggacca tttccaataa aactggtaat taaaaaaata
      301 tgtgccttga gattaaactc gtttaataaa ggatatacct gaaaccagtt gtcaaggtat
      361 ccatcatcaa atgtcaacat aacgcttttc cgggggagtt ttccgccccg ataaaaaaac
      421 tccagttcat ctgatgacag tgttttccag ttgttttcag ccaaccattt tatttgcttc
      481 cggaatgtca cgggagaaag tgtaaccagt ccggggcaat gactgacatg atgatacatt
      541 aataccggaa catgattggc tttgaacaaa atgccccctt cgtttaacat ttaaagtttc
      601 tggcacctag caagcagaga caaaaggaaa acccaataaa gctggtgcat tttatttcca
      661 gatactggat gaataacata gcaaacccat ggcatatttg atataattta ctaaattaaa
      721 catcaacaat t
//
LOCUS       DAZSNO010000455          724 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZSNO010000455 DAZSNO010000000
VERSION     DAZSNO010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.464"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(61..724)
                     /locus_tag="ACHWN2_004571"
                     /pseudo
     CDS             complement(61..724)
                     /locus_tag="ACHWN2_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          208 a          147 c          138 g          231 t
ORIGIN      
        1 tatttttcag tgggtatatc tgttcagaat atttcacgta gcacacggat atataagaaa
       61 tcattcttcc tctggatagc ttgaacttaa gagcatatcc tgcgtgaatt ggtcattagc
      121 tgattgattg aagctaaaga aagatggtgc atccggtcta acagttgctt caccagtgat
      181 attaactgat aaaggcttct tagtttctgc tgttaaagcg tctacctttc ttcctaactc
      241 tgatacagcc atagctaatt gagcattgct attaaccatg tcacctgtga tagctgatgg
      301 agttgattgt gtgttattta cgaagttatt catcggttct tggaagttag caattttatt
      361 agttgcacca ttaaaccagt cagcgataga actattggct tagatgaatc acctttgtat
      421 tcagggttga agtttgtttc tgtgccaata cctgattgat gtaagttcgg attcttagct
      481 gcttcagggt ttaaattatc aagagcaaaa tttaccaact cagcataaag gacaactcta
      541 ccgactttat ttagcgcacc acctgaagaa ccagccttgc cagctttagc tttattaaca
      601 gcgtcaccga atgaagtaac agcgtttgca aggtttaaca agcctcctgc tacatttgca
      661 gccttttgag cagtaacagc cacagcaagc atcttcagac catcaattaa cccatcagat
      721 aaac
//
LOCUS       DAZSNO010000456          716 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZSNO010000456 DAZSNO010000000
VERSION     DAZSNO010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.465"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            61..>716
                     /locus_tag="ACHWN2_004572"
     CDS             61..>716
                     /locus_tag="ACHWN2_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933749.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     T"
BASE COUNT          276 a          142 c          113 g          185 t
ORIGIN      
        1 ctgttctaat tatccaattt aaaaacatta gcataaaaca gatgatgaat aaggaatgtt
       61 atgtcaattg actttacccc aggtataata aatacatatc acggcgatat ttataactgc
      121 acaacaaata ccgataatgt taaaacgcca gacacaccga agtggccttg tgataattgg
      181 gaagaacaac aacccattaa ttccacattc tctggagaag gatacaaccc tgaacaattt
      241 gatttagcgc aacaacaact ccaacaaatt aatgcatgcc acacgaacac cacatataca
      301 aatgcagact actcaaaagt tgtggttcaa cttgtaagtc ttattaataa cattgaaacg
      361 ataagttcga caccacttac gcaacagaca caatcaatac ttaatcagat tcataacatt
      421 agatacgaaa aggataagga ttctgtatgt caaatcattg ttgtcgttaa tccagaaccc
      481 aacaatccaa tcataactaa aattctcgta gaagaaggaa tacccgaaag attcagtgtc
      541 caaaccgttt cttccgataa taaaaatttt gctgggcaac gagctgactt accaactgat
      601 ataagagaca ttcaaagctt gtacttaaaa atggcgaagc tgtatattga gcacagtaaa
      661 aatgaaaata ggatggaagc ccttgctgga tgcgacttta ttgatttcaa catgac
//
LOCUS       DAZSNO010000457          715 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZSNO010000457 DAZSNO010000000
VERSION     DAZSNO010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.466"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..197
                     /locus_tag="ACHWN2_004573"
     CDS             <1..197
                     /locus_tag="ACHWN2_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR8933750.1"
                     /translation="FRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGL
                     PPNESENRYWKNSNSVASFC"
     gene            564..>715
                     /locus_tag="ACHWN2_004574"
     CDS             564..>715
                     /locus_tag="ACHWN2_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR8933751.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          184 a          181 c          162 g          188 t
ORIGIN      
        1 tcttcaggag tctgaagaac gaatggatgc cggtggtggg ttacgtaagc ttcagcgagg
       61 cagctcacgc cataacggac tatatcgttg gatattacag tgcactaaga ccgcacgaat
      121 ataacggtgg gttaccccca aacgaatcgg aaaatcgata ctggaaaaac tctaactcgg
      181 tggccagttt ttgttgacca cttcatctag ggtctctcta taggtcttct ctatggtctc
      241 tctataggtc tctctagggt ctcctctagg cattaccccc agaagctaac tctatggccc
      301 tctatggtcc tctatggccc tgtacgtcat tctttaggat gccctgtagt ttcgtatagc
      361 ctgatgccta gagagaccct agagtggctc ctagagagac cctcccagta gctccgcaca
      421 gctacatcgt catcatcata gtattcaatc tcaaagtcat tctgtttgca tcctgttgtc
      481 ctgctggtct cccttcgctt ggggactttg agtaggtcag tgggggtgcg acttcagggt
      541 gcaaacaaaa aggaaatcaa gcgatgtctc aacaaccaca acaaccacaa caagaaatca
      601 gcatcattaa cctcgaccag cttgtgtcca tgacctcagt tgaaattgct gagctgacag
      661 gcaaggagca ccgaaatgtc cttcgtgata ttcgtaagat gctcgaagaa cttaa
//
LOCUS       DAZSNO010000458          712 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZSNO010000458 DAZSNO010000000
VERSION     DAZSNO010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.467"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(6..82)
                     /locus_tag="ACHWN2_004575"
     tRNA            complement(6..82)
                     /locus_tag="ACHWN2_004575"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Ile,seq:cat)
     gene            complement(272..>712)
                     /locus_tag="ACHWN2_004576"
     CDS             complement(272..>712)
                     /locus_tag="ACHWN2_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR8933752.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          180 a          197 c          132 g          203 t
ORIGIN      
        1 gtggttggtg gcccttgctg gacttgaacc agcgacctgg cgattatgag tcgctcgctc
       61 tgaccaactg agctaaaggg ccggaggcag aataataacc atatgtcatc acatctgcaa
      121 actcatctga tcaccagcac gtttaacgtc ctgtgccgtt tttcaggtat aaaaaaaacc
      181 gcataaagcg ggctctttca aatgtccatc tctgctattc gcctcgcggt acagctttgc
      241 gaagcgtaca agaattgaag cagtttttac gtcaaaaagc aataactttt ttctctatac
      301 caaaagccat aaccattggt ttgtacaaaa taaattctgc cacctttagc caatgctcaa
      361 tgcgtctttc acaggttctt aaactccatt tcgggtgtgc atcattcagc agttcagcca
      421 ttttgcattt ggtcatcccc cgcccctcat accgttgccg aaggacgcta atcaatcctg
      481 gatgctctgc cagcacctca cttatgaccc gatcaataca taacgcctct gcatcagtac
      541 aatgcgccag ccagctcttt tgcttaccgt taatcatatc ccgcaaaaaa gcctcaagtt
      601 cagacttgtt cagacctgct tttttcatcc tccggagcgc ctcgttaatt gccgtttttg
      661 tcagcttttt agaggtcaac atcaggttaa acatattccc cgtcttaccg cc
//
LOCUS       DAZSNO010000459          707 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZSNO010000459 DAZSNO010000000
VERSION     DAZSNO010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.468"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..373
                     /gene="tnpA"
                     /locus_tag="ACHWN2_004577"
     CDS             <1..373
                     /gene="tnpA"
                     /locus_tag="ACHWN2_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR8933753.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
     gene            373..>707
                     /gene="tnpB"
                     /locus_tag="ACHWN2_004578"
     CDS             373..>707
                     /gene="tnpB"
                     /locus_tag="ACHWN2_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8933754.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAG"
BASE COUNT          165 a          194 c          193 g          155 t
ORIGIN      
        1 gcggcctaat tttccttatg aatttaaaat cgctttagtg gagcagtcac tgcagcccgg
       61 agcctgtgtg gcgcagatcg cccgtgaaaa cggaatcaac gataacctgc tcttcaactg
      121 gcgccatcaa taccggaaag gtggcctgct gccttccgga aaaaatatgc ctgcactgct
      181 tcccgtgacg ttaacgccgg agccggataa taaaatcccg gctccggcac aggaaccaga
      241 gcagataaat acaccgtccg acagtctgtg ctgtgagctg gttctgccgg ccggaactct
      301 caggcttaaa ggtaaactga cgccggcgtt attacagata cttatccgcg aaataaaagg
      361 gagcagccac tgatgatatc tttccctgca ggttcgcgta tctggctggt tgcaggtatc
      421 accgatatgc gaaatggctt taacggcctg gtctcaaaag ttcagaacgt cctgaaggat
      481 gacccgttct ccgggcatct gttcatcttc cgcggacgcc ggggtgacca gataaaagtg
      541 ttgtgggctg acagtgacgg actgtgcctc tttaccagac gcctggagcg gggccgcttc
      601 gtctggccgg tcacccgtga cggcaaggtg caccttactc cggcccagtt atccatgctt
      661 cttgagggca tcgactggaa acacccgaaa cgaacggaac gcgctgg
//
LOCUS       DAZSNO010000460          706 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZSNO010000460 DAZSNO010000000
VERSION     DAZSNO010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.469"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            88..153
                     /locus_tag="ACHWN2_004579"
                     /pseudo
     CDS             88..153
                     /locus_tag="ACHWN2_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..629
                     /locus_tag="ACHWN2_004580"
     CDS             381..629
                     /locus_tag="ACHWN2_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR8933755.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          226 a          150 c          160 g          170 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcac
//
LOCUS       DAZSNO010000461          706 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZSNO010000461 DAZSNO010000000
VERSION     DAZSNO010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.470"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>706)
                     /locus_tag="ACHWN2_004581"
     CDS             complement(<1..>706)
                     /locus_tag="ACHWN2_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HFR8933756.1"
                     /translation="PELKNLNALITELKEQNCYVVVVDNRTNFTLKDKLADIEKVHLI
                     CLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLSCYMDNENAKIAGP
                     VFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTMVSKEVFEIVGMMD
                     EELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGINIPIHSPVRRYYR
                     VRNAFLLLRKNHVPLLL"
BASE COUNT          225 a          134 c          101 g          246 t
ORIGIN      
        1 gagtaaaaga ggcacatgat tttttctaag caaaagaaat gcatttctta ctcgataata
       61 acgacgtact ggcgagtgaa ttggtatgtt aatcccacag atttttactg acttatcccc
      121 aatagcatga accatttcta tatcaggaat gatatgaact aatatgccat agtttaagca
      181 tctaagacac cattctgtat cgacataatc aataaaaagt tcctcatcca tcattccaac
      241 aatctcaaaa acttcttttg aaaccatggt tccggatgag attgttactg aacttttaaa
      301 cggtgtctgt ccttcagtga catgaatttt ctctcgaaga ccattttttt tgatattaca
      361 aataggataa taatgtgact tatctctatc aataaaaact gggccagcta tttttgcatt
      421 ttcattatcc atatagcagg aaagtttttt gataaactca ttgcgaatac ggctatcttg
      481 atcaaaaaaa attatcttct ctgccccttt ttcaagagaa tatcgaatcc caatattttg
      541 tgcttttgct ataccttcat tcctaccaag acaaattaaa tgaacttttt caatgtctgc
      601 taatttatct ttcaaggtga aattagtcct attatcgact actacaacgt aacaattttg
      661 ctcctttagt tcagtgatca gtgcattcag atttttaagt tcggga
//
LOCUS       DAZSNO010000462          704 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZSNO010000462 DAZSNO010000000
VERSION     DAZSNO010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.471"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..443)
                     /locus_tag="ACHWN2_004582"
     CDS             complement(<1..443)
                     /locus_tag="ACHWN2_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR8933757.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttca
//
LOCUS       DAZSNO010000463          702 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZSNO010000463 DAZSNO010000000
VERSION     DAZSNO010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.472"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            45..>702
                     /locus_tag="ACHWN2_004583"
     CDS             45..>702
                     /locus_tag="ACHWN2_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR8933758.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     L"
BASE COUNT          259 a          145 c           98 g          200 t
ORIGIN      
        1 tctcccggca agaatctctg attgagttaa gtgcccatca caatatgatc actgacctta
       61 tattacacaa tcatcccagg atgaaaacaa tcactttaaa cgacaaccat attgcacatt
      121 taaacgccaa aaacactaca aaactggaat atttaaactt aagcaataac aatttactgc
      181 caaccaatga cattgatcaa ctaatatcat caaaacatct ttggcatgta ttagttaacg
      241 gcatcaacaa tgatccactt gcacaaatgc agtactggac tgcagtaaga aatataattg
      301 atgacactaa tgaagtgacc attgatttat caggacttaa tttaaccact caaccaccag
      361 ggctgcaaaa cttcacctct atcaatcttg ataataacca actcacacat tttgatgcaa
      421 ccaactacga tagactcgta aagctaagtc tgaatagtaa tgctcttgag tcaataaatt
      481 ttcctcaagg cagaaatgta agtattacac atatatctat gaataataat gctctcagaa
      541 atattgatat agataggctt tcatcagtta cttattttag tgcggcacat aatcaactag
      601 agtttgtgca attagaatct tgcgaatggc tgcaatacct gaatctcagc cataatcaat
      661 taactgatat tgttgcaggt aataaaaatg aactcttact gc
//
LOCUS       DAZSNO010000464          699 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZSNO010000464 DAZSNO010000000
VERSION     DAZSNO010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.473"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            82..555
                     /locus_tag="ACHWN2_004584"
     CDS             82..555
                     /locus_tag="ACHWN2_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR8933759.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          228 a          126 c          147 g          198 t
ORIGIN      
        1 taatcatgcc gggtctggct ggctaacacc ccattataag gggtacgaat aaagtaacgg
       61 gggggagatg aaccctatgc aatgtcctgg ttaatatctc agtgtgctca tcagtgcact
      121 gataataaaa agacggagac agatgccatt tacgataaag ttcggagttc ttatttgctt
      181 tcatgtattc tgaaaaaaaa caaaaatgtt gggcttattc ttcatgcacc atcatttgtt
      241 tctgtgtctg aaaaaatcgc cagaattgtg atggcaaatt actcccgtaa ctggagtaat
      301 tccgagttgg ccagtgcggt tttaatgagt gaatcttctt taaaaagaag aatgtacaaa
      361 gaggtcggtt ctataagtac atttgtacat aaaataaagc ttacagaagc tatcaggaaa
      421 ttacgaagga caaatactcc catttctgtc atatcatctg agttaggtta cagttcacca
      481 tcgtattttt ctaaagtctt ctttaaatat ttaaagacat accctcaaaa cataaggaag
      541 aaaaatggtc ggtaatgtca ccaattagga ggacttgcag agccgctttt ttacgatact
      601 gagatcaaac agacataatc tatgcggaga tttacagcag ggcatggttc acaaatctga
      661 cagcgatgaa ttatcggcac tgcgggcaga aaatgctcg
//
LOCUS       DAZSNO010000465          695 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZSNO010000465 DAZSNO010000000
VERSION     DAZSNO010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.474"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            32..493
                     /locus_tag="ACHWN2_004585"
     CDS             32..493
                     /locus_tag="ACHWN2_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR8933760.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>695)
                     /locus_tag="ACHWN2_004586"
     CDS             complement(534..>695)
                     /locus_tag="ACHWN2_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR8933761.1"
                     /translation="ILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGL
                     AARQHVKKS"
BASE COUNT          204 a          152 c          159 g          180 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaat
//
LOCUS       DAZSNO010000466          690 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZSNO010000466 DAZSNO010000000
VERSION     DAZSNO010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.475"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            52..690
                     /locus_tag="ACHWN2_004587"
                     /pseudo
     CDS             52..690
                     /locus_tag="ACHWN2_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          190 a          152 c          134 g          214 t
ORIGIN      
        1 taattcatta taaataataa tgaaataacg ccttttattg ataatttaca tatgaaaaaa
       61 ttttcctttt gttatattat ttctgttttc actccctctg attattttta ccaaccctgg
      121 gtcactgcat tatcgccaac gccccgacac gtaagtcctg agcaattaga aaaaacagtt
      181 cgttatctta cacaaactgt gcatccacgt aatgccggca atattgataa tctgaatagg
      241 tccgcagagt atataaaaga gttctttgtc agtagcggtg ccagagttac cgcgcagggc
      301 gtccccgatt ggctattacg actctgcgcc tggaagccag aattaccctt acccggcaat
      361 gtcctggctt tatcccgatc ggggagactt tattgctgtg gtcggcagaa tgcaggatat
      421 caacgccgtt cgtcaggtaa aagcggcatt gttgtcatcc cgggatttat ctgtttattc
      481 tatgaatgcc ccagggttta ttcccggtat tgatttttct gatcacctca attattggca
      541 acacgatatt cccgccgtaa tgattactga caccgctttt tatcgtaata aacaatacca
      601 cttgcccggt gataccgcag acagattgaa ttatcagaaa atggctcagg tggtagatgg
      661 tgttataacc ttgttataca acagtaaata
//
LOCUS       DAZSNO010000467          683 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZSNO010000467 DAZSNO010000000
VERSION     DAZSNO010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.476"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>683
                     /locus_tag="ACHWN2_004588"
     CDS             <1..>683
                     /locus_tag="ACHWN2_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HFR8933762.1"
                     /translation="DVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQSEIY
                     ARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTK
                     TGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLY
                     ETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAV
                     RKARSVQLM"
BASE COUNT          179 a          169 c          205 g          130 t
ORIGIN      
        1 tgatgtcatc gttcaggcac cacttccccc taaaccgatc gaacgcggtt atgccagtgc
       61 agggttactt gcacggatcc tggtcagcaa atatatggaa catatccctt tatatcgcca
      121 gtcagaaata tacgcgcgac agggcgtgga gctgagccgt aataccatgg tgcgctgggt
      181 atcagaaatg gcagacaaac tccgtcctct gtatatagcg ctgaatgact atgttctgga
      241 ggcaggaaag gtgcacgcag atgacactcc ggtgaaagta ctggccccgg ggaacggaaa
      301 gacgaaaacg ggtcgtctgt gggtatacgt cagggatgat cgtaatgcgg gttcatccct
      361 gccggcagcc gtctggttcg cgtattcggc agatcgcaaa ggagaacatc cgcagctcca
      421 cctggcaaag tatcagggcg tactgcaggc tgatgcctat gcaggttata acgtactgta
      481 cgaaacgggc cgggtgaagg aagccgggtg cctggcccac gcccgccgaa aaatccatga
      541 cgaggatgtg cgccgtccga cagaaatgac tcaggaagcg ctcagacgga tagcagagtt
      601 atacgacata gaagcggaga tacgtggcag tccggcagag gaacggcttg cagtcagaaa
      661 agccagaagc gtccagttga tgc
//
LOCUS       DAZSNO010000468          677 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZSNO010000468 DAZSNO010000000
VERSION     DAZSNO010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.477"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(169..381)
                     /gene="cspH"
                     /locus_tag="ACHWN2_004589"
     CDS             complement(169..381)
                     /gene="cspH"
                     /locus_tag="ACHWN2_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR8933763.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT          207 a          129 c          141 g          200 t
ORIGIN      
        1 agtggtacta taactggctg ttaaatggca tttataacta ttaggtgcag agatattcgc
       61 ttaaatggat tagtttctta gtggaaaagt aactgatgtt ataatgtatg gtgttgacta
      121 ttgaactatt ccaataaaac cagtcagctt taaacaagca gcgtcatatt aagagagata
      181 aacatttgcc gctgttggtc ctcgcaggcc atttacgcgg caaaattcca cgcgtaatcc
      241 tggtataagc acttctgcgt cgcggggagt gaatgcggaa atatggacct ggacttcttt
      301 acgaccgtcg gaggggataa tgaatccttt gccgcttttg cgatcaaagg ttttgacaat
      361 tcctgtcatt ttacgggaca aacaaattcc ttactgaaaa tactgcgctg cactatacgg
      421 ggttaataaa ataaagccag cgatatttaa gaccgccgga cggctaaaat aaaatttgct
      481 taatctcaat tatcatgcct taatagctgc gtcggtttga aagacagaca gcatacaaag
      541 tagtttacta aagcagttct cattatcagg cattatcccc ttcttttgag tctctctcct
      601 gaacactaag tggtttctgt attaaagccc tgtttgccga aaggcccaaa atgaaggaag
      661 taaaatatgt ctaataa
//
LOCUS       DAZSNO010000469          668 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZSNO010000469 DAZSNO010000000
VERSION     DAZSNO010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.478"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>668
                     /locus_tag="ACHWN2_004590"
     CDS             <1..>668
                     /locus_tag="ACHWN2_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR8933764.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAZSNO010000470          663 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZSNO010000470 DAZSNO010000000
VERSION     DAZSNO010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.479"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            163..648
                     /locus_tag="ACHWN2_004591"
     CDS             163..648
                     /locus_tag="ACHWN2_004591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933765.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLLNVT
                     K"
BASE COUNT          217 a           84 c          146 g          216 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 tta
//
LOCUS       DAZSNO010000471          656 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZSNO010000471 DAZSNO010000000
VERSION     DAZSNO010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.480"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>656
                     /locus_tag="ACHWN2_004592"
     CDS             <1..>656
                     /locus_tag="ACHWN2_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933766.1"
                     /translation="VLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRNLPSGVVP
                     AVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHR
                     YGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELS
                     WWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPL
                     P"
BASE COUNT          144 a          179 c          189 g          144 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcc
//
LOCUS       DAZSNO010000472          651 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZSNO010000472 DAZSNO010000000
VERSION     DAZSNO010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.481"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(29..397)
                     /locus_tag="ACHWN2_004593"
                     /pseudo
     CDS             complement(29..397)
                     /locus_tag="ACHWN2_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          194 a          114 c          144 g          199 t
ORIGIN      
        1 ttgtctggag attcaggggg ccagtctaat cagttgtcat gcactcggaa taaatcatac
       61 cgtgaatagt gctgtttttg attgtcagac tattggcgtt ggtattggta gaaataccgt
      121 cgtccagata gtaggtagaa atcacgccgt taacggtagc gttattaatg accgggtaga
      181 tatcgccgtt gtaatagcta tcagcggcat agttgttcca gcctacataa ccatcatagt
      241 aaacgtcatc agcataggtg gcgtcgttgt agtgatggaa agtattgtaa gtttgaccag
      301 aaatatcgtt ggtcgctgca ttagcctgag aggtgatagc cagagtgcaa gccaatgcta
      361 attgtgatac tacaagtttc tttttccagg agtgcatttg tcatccctcc tcagggacgt
      421 aataagttga tccatcaatt atcaatgcat agaaaacata ttatgtttcg cgctaacgat
      481 tatgctgcgt tacagacgaa tggttcaatt attctttccc ctaagtccga atgtggacat
      541 atataattta tggagaaaaa cacgcatcaa attgataaaa ggacgatatt tcagtatgtt
      601 gcagataatg ttatttttga tgcatacgcg ttataaatat caaaaataca a
//
LOCUS       DAZSNO010000473          649 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZSNO010000473 DAZSNO010000000
VERSION     DAZSNO010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.482"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(468..>649)
                     /locus_tag="ACHWN2_004594"
     CDS             complement(468..>649)
                     /locus_tag="ACHWN2_004594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933767.1"
                     /translation="QRYIDVFFMVKNHIFFYIHNVMIVCSAFWGCQRRTGCADHMRRM
                     RCPGDSPPQYSVFSF"
BASE COUNT          250 a          160 c           86 g          153 t
ORIGIN      
        1 tcatctccac tggcgagcac ggtcagcaca caaacaaagt tagtcctcct gcttaatcat
       61 tgcgtatcag aaactaaaaa agaaaaaatt ctgcagacaa cttaacaatc ttcatcacat
      121 ttcctgaaga cagacatatc cacaaacggt agtcagcgtt gaattaacaa gtcttacaga
      181 gctatacgta gcttataaag ccactcaatg ttcatcatca cccgactact tatctggaat
      241 accaccagta taaactcatc aataaaaaca cctgttgtta aaataagaat actgtcagca
      301 gtaaattatt aaaacaacat ctgtccacac tatctgttct tttaacgcca acggcagcga
      361 aaatagtgca tcacagcctt cttttttctt tcgccaccac tcagacaaac acaacactca
      421 cacccacacc acaaaacaga atcaaaacat aaaattaaca ataacagtca aaaggaaaac
      481 acagaatatt gaggtggact gtcaccagga cagcgcatac gacgcatgtg atcagcacat
      541 cccgttcttc gctggcatcc ccaaaatgca gaacaaacaa tcataacatt atgaatataa
      601 aagaaaatat gatttttcac catgaaaaaa acatcaatgt atctctgta
//
LOCUS       DAZSNO010000474          644 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZSNO010000474 DAZSNO010000000
VERSION     DAZSNO010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.483"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            2..>644
                     /locus_tag="ACHWN2_004595"
     CDS             2..>644
                     /locus_tag="ACHWN2_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR8933768.1"
                     /translation="MRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVTNSCGS
                     RACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVCRLAVE
                     NLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFLKDAIR
                     SRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKKT"
BASE COUNT          152 a          163 c          200 g          129 t
ORIGIN      
        1 tctgcgcgat atcgaggttg aggctgtcac aaaaatgctg gcctgcggca cgcggatact
       61 gggcgtgaag gaatacatct gcgataaacc tgagtgcccc cacgtcagat acgtcactaa
      121 ctcatgcggc agccgtgcct gcccgtcctg cggaaaaaag gccacagacc tgtggatagc
      181 gacacagctg aatcgtcttc ctgactgcga ctgggtacac ctggtcttca ccctgccgga
      241 cacgcagtgg ccggtgttcg aaagcaaccg gtggctgctg aatgacgtgt gccgtctggc
      301 ggtggagaat ctgctgtatg ccgcccggaa acgggggcag gaacccggta tcttctgcgc
      361 catccacacg tatggccgtc gtctcaactg gcatccgcat gtacatgtgt ctgtaacctg
      421 tggaggtctg aataagcatg gtcagtggaa aaagctgagc ttcctgaaag acgcgatacg
      481 ttcgcggtgg atgtggaata tgcggcaacg gcttctgaaa gcgtggtcag aggggctggc
      541 gatgccggag tcgttgtcac atatcacgac ggaatcacag aggagaagcc tggtgctgaa
      601 agccggagga aaatactggc atgtgtacat gtcgaaaaaa acgg
//
LOCUS       DAZSNO010000475          642 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZSNO010000475 DAZSNO010000000
VERSION     DAZSNO010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.484"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(18..>642)
                     /gene="wzx"
                     /locus_tag="ACHWN2_004596"
     CDS             complement(18..>642)
                     /gene="wzx"
                     /locus_tag="ACHWN2_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFR8933769.1"
                     /translation="KTFKRLFFFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYS
                     APAEVILKLGIIPAAIGRAVFPRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLY
                     SGLVLKIWFGENYQINSFNILNVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAEL
                     VPYLALLYFMVEKYGLLGAAISWSIRVILDALLLQWLYTRMCSVYEN"
BASE COUNT          236 a          125 c           90 g          191 t
ORIGIN      
        1 tcagtaattg gttaatttta gttttcatat acagaacaca ttctagtata aagccattgt
       61 aataacagag catctaaaat tacacgtatg ctccaggata ttgcggcacc cagtaaccca
      121 tatttttcga ccataaaata caaaagggct aaataaggaa ccaattcagc acaatgaatc
      181 aaagcagtaa ttttagattt tcctaatgcc tggattgcag agaatggtat cattgccagc
      241 gcattgaaga aaaaaccgat caataacaca tttaatatat taaaggaatt aatttgataa
      301 ttctcaccaa accatatttt caatacaagc cctgaatata acaagcctat gattatcacc
      361 ggcaaacaga ttagaaacat taaaagcaat gatttattta cattacgttt aaattcttta
      421 aagtctttga tgttacttaa ccttggaaac actgccctcc cgattgctgc aggtattatt
      481 cctaatttta gaataacctc cgctggtgca gaataaaatg caactttatc tgcccccata
      541 atatttgaca ctataaatcg atcaaaatac accatgaccg gacttatgat attacttact
      601 gtcatccaac cgccaaagaa aaagagtcgt ttaaaggttt ta
//
LOCUS       DAZSNO010000476          629 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZSNO010000476 DAZSNO010000000
VERSION     DAZSNO010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.485"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(124..>629)
                     /gene="spa40"
                     /locus_tag="ACHWN2_004597"
                     /gene_synonym="spaS"
     CDS             complement(124..>629)
                     /gene="spa40"
                     /locus_tag="ACHWN2_004597"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFR8933770.1"
                     /translation="RLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKREY
                     IEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPFI
                     SLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVWL
                     EQVENTH"
BASE COUNT          211 a          124 c           84 g          210 t
ORIGIN      
        1 tattgggttc aatacctcat tagcaatgga gttaatgatg gaagattcta tttcaaattc
       61 agactgatca atacctattt tattacagaa attaactccc atataacaca tctcctttac
      121 ttattaatga gtgttttcaa cctgctcaag ccaaacaata agacgtagga cttcatccaa
      181 gtgttcaaaa tcaacaaaac tatattttgt atgtgtttta tatagttttc tagctaattt
      241 cacatcacgc acagtcggta taccaacttc atttgcatat tttctgacag ccaaggcaca
      301 ctggttagtt tcaatgagag aaataaaagg tgcaggcgct atttctggat taaaataaat
      361 accaattgca atatgagtcg ggttcattac cactaatttt gaattacgta tatcagattt
      421 agtctgctct gaaagaatct cgatatgcaa ctcacgcctt ctcgactttg tctcaaagtg
      481 tccctcttgc tctatatatt ctctttttat ctcctgttta tccatcatca tatctttcat
      541 gtataaaatg aactcaatca caaagtcaag aataataaca agaatagaaa atgccaagaa
      601 aaataatatt atatccttaa acagcctcc
//
LOCUS       DAZSNO010000477          623 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZSNO010000477 DAZSNO010000000
VERSION     DAZSNO010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.486"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..251)
                     /locus_tag="ACHWN2_004598"
     CDS             complement(<1..251)
                     /locus_tag="ACHWN2_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFR8933771.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>623)
                     /locus_tag="ACHWN2_004599"
     CDS             complement(272..>623)
                     /locus_tag="ACHWN2_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR8933772.1"
                     /translation="TVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRH
                     RKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRR
                     LSESVPCMPHKKAG"
BASE COUNT          141 a          145 c          141 g          196 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gta
//
LOCUS       DAZSNO010000478          622 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZSNO010000478 DAZSNO010000000
VERSION     DAZSNO010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.487"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            63..>622
                     /gene="virB"
                     /locus_tag="ACHWN2_004600"
     CDS             63..>622
                     /gene="virB"
                     /locus_tag="ACHWN2_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR8933773.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIAS"
BASE COUNT          227 a          116 c          115 g          164 t
ORIGIN      
        1 tcttgctcac actgcattta acttttgtca atataaatca atatgaataa acagggtgtg
       61 atatggtgga tttgtgcaac gacttgttaa gtataaagga aggccaaaag aaagagttta
      121 cactccattc tggtaataaa gtttccttta tcaaagccaa gattcctcat aaaaggatcc
      181 aagatttaac cttcgtcaac caaaaaacga atgtacgcga tcaagaatcc ctaacagaag
      241 aatcattagc cgatatcata aaaactataa agctacaaca attcttccct gtaataggaa
      301 gggagattga tggtagaatt gaaattctgg atggcactcg tagaagagca tctgcaatat
      361 atgcaggagc agatcttgaa gttctatatt caaaagaata tatatctact cttgatgcca
      421 gaaaactagc aaacgatata caaacagcaa aagagcatag catccgagaa cttggtattg
      481 gtcttaattt tctgaaagta tcagggatgt cctataaaga catagccaaa aaagagaatc
      541 tgtctcgcgc gaaagtcact cgtgcctttc aggcagcaag cgttccacag gaaataatat
      601 ctctatttcc aatcgcgtca ga
//
LOCUS       DAZSNO010000479          604 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZSNO010000479 DAZSNO010000000
VERSION     DAZSNO010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.488"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..294
                     /locus_tag="ACHWN2_004601"
     CDS             <1..294
                     /locus_tag="ACHWN2_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR8933774.1"
                     /translation="SAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFE
                     SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            300..>604
                     /locus_tag="ACHWN2_004602"
     CDS             300..>604
                     /locus_tag="ACHWN2_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933775.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVM"
BASE COUNT          174 a          135 c          153 g          142 t
ORIGIN      
        1 agcgcttatc cgatttttga tcgtattgaa gaaatggcat gggctcgtca ctaccagcag
       61 atcgttcgtg aagaaaaaga aacggaactg gcggacgacc tggaaaaagg tctgcctcag
      121 cacctgtttg aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca
      181 atcagccacg catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg
      241 tacatggctg aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta
      301 tggcgctttt tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt
      361 ttgctggcga cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta
      421 tgcggcaacg gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag
      481 gctctgactg ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa
      541 cccgctcgtt tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa
      601 tgat
//
LOCUS       DAZSNO010000480          603 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZSNO010000480 DAZSNO010000000
VERSION     DAZSNO010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.489"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            28..603
                     /locus_tag="ACHWN2_004603"
                     /pseudo
     CDS             28..603
                     /locus_tag="ACHWN2_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004243150.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
BASE COUNT          175 a          110 c          132 g          186 t
ORIGIN      
        1 aattcttgtt atagagaagc agtcgatatt catagctcac ttgagctttc atttatattg
       61 cataaatatc atttgcgtgt agatgagaag aaatctcgaa atgtattcag cgccatacgt
      121 gatgagctca tccttgatgg ggattcacga caaaacttag ctactttctg tcaaacatgg
      181 cttgatgatg aaatccgtga gttgcttaat ttagctattg ataaaaacat gatttataaa
      241 gatgaatatc cacaaatggc ggaacctgaa ggtcgctgtg tgcgtatgct ttccgattcg
      301 tggagtttcc ccgattcgcg acacacctta ggttgctcaa ccattggttc atctgaagct
      361 gctatgctcg gccgcttggc gttaaaatgg cagtggtgta aaaaacgcga agtacaaggt
      421 aaatcaaccg aacctgattt gcgatcctgt gcaaatatgc tggcataaat tctgccgtta
      481 ctgtgatgtg aaattgtgtg aaattccact tgatgatcat cacctgataa tgaatgctaa
      541 agaggttttg aagcatgtcg atgaaaacac tattggtgtc gttacgactt taggtattac
      601 tta
//
LOCUS       DAZSNO010000481          593 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZSNO010000481 DAZSNO010000000
VERSION     DAZSNO010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.490"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>593)
                     /gene="ospC2"
                     /locus_tag="ACHWN2_004604"
     CDS             complement(<1..>593)
                     /gene="ospC2"
                     /locus_tag="ACHWN2_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR8933776.1"
                     /translation="KNISCVTNSSSSGISEKHLDHCADTVKSFLRKSIAAQSYSKMFS
                     QGTSFKSLNLSIEAPSGARSSFRSLEHLDKVSRHYLSEIIQKTHPLSSDERHLLSIII
                     NSDFNFRHQSNANLSNNTLNIKSFDKIKSENIQTYKNTFSEDIEEIANHDFVFFGVEI
                     SNHQETLPLNKTHHTVDFGANAYIIDHDSPYGYMTLT"
BASE COUNT          188 a           87 c          115 g          203 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt ttt
//
LOCUS       DAZSNO010000482          589 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZSNO010000482 DAZSNO010000000
VERSION     DAZSNO010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.491"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          185 a          114 c          102 g          188 t
ORIGIN      
        1 cttctgggga acgctgaaaa atgaaagcct gagccactat cgttttaaga gccgggatat
       61 ttcgtcagca tacgggaaaa ctgattgagg tcggcctcag ttttgaggcc tttagccagt
      121 tcagccgcaa gggctttaag cttcttttcg tccataattt acctgtctcc gttgttggag
      181 taaacatatc aaaaacaggc aattacacaa tttaaattac agtctcaaaa caaacccaac
      241 ggtcaatata gtttaccagc aagcttacta gcaaagtgct aatcatttgg tatctttcgt
      301 gctattctcc ttttacgtaa gtacttaagg ggaacgtatt tactactttt aaagaaagct
      361 ccgttactat ctgaaatgca atgagataat aaacgattac tctcggttag tatctcatga
      421 tcatttacag actttgcata atcgcgaata ctatataaca gagttttaac attacaggat
      481 gaatagtctc tcttatcaac tcctaataaa agaaggatta tggtcagtcg tttttgctgt
      541 cttatcatat attatttagt gcagttccta acaaaaacat aacaatttt
//
LOCUS       DAZSNO010000483          584 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZSNO010000483 DAZSNO010000000
VERSION     DAZSNO010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.492"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..150
                     /locus_tag="ACHWN2_004605"
                     /pseudo
     CDS             1..150
                     /locus_tag="ACHWN2_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005284207.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(275..556)
                     /locus_tag="ACHWN2_004606"
     CDS             complement(275..556)
                     /locus_tag="ACHWN2_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFR8933777.1"
                     /translation="MTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAV
                     NALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          189 a          112 c          104 g          179 t
ORIGIN      
        1 gctggataag atcataacga tacaccgggt taaacaggtg gtcactgctt cactgaagct
       61 gcgtgattat gatggtcagg tagcagaagc tatgcccatg atacaaaaat gacgaaagcc
      121 gaaatgtctg aaagcgtgtg tattacctga aaatatgacc ggctccgggg tacgtaaccg
      181 attcaacaaa gccaatcgat attacaaaac gagagggact atatatttcg ttttcagata
      241 agactccttg agagattatc ttttatgtaa aatctcaaat atcacctgat gtaatgtcaa
      301 tgataatatt aagtgcgctc tgaacaacat ctttcttggt gtgattaaca tctgacaaaa
      361 agaacatcag cttctttatt atgttcttgt tgtttattat ttcacgctca cagaataaag
      421 cgttgactgc ctcttctatg acatttctct cgtaaaaaaa ttgagagtta gcttcatata
      481 aaatattggc caatttttca ccaaaaacca tattataata atcactgtgc atctttctcc
      541 cccagggttc agtcaataat tttaccatgc ctaattatga cagg
//
LOCUS       DAZSNO010000484          583 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZSNO010000484 DAZSNO010000000
VERSION     DAZSNO010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.494"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>583
                     /gene="istA"
                     /locus_tag="ACHWN2_004607"
     CDS             <1..>583
                     /gene="istA"
                     /locus_tag="ACHWN2_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFR8933778.1"
                     /translation="SRNTVKRYLQAKSEPPKYTPRPAVASLLDEYRDYIRQRIADAHP
                     YKIPATVIAREIRDQGYRGGMTILRAFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTM
                     RNGRSPLHVFVAVPGYSRMLYIEFTDNMRYDTLETCHRNAFRFFGGVPREVLYDNMKT
                     VVLQRDAYQTGQHRFHPSLWQFGKEMGFSPRLCR"
BASE COUNT          127 a          158 c          150 g          148 t
ORIGIN      
        1 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
       61 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      121 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      181 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      241 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      301 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      361 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      421 gtaatgcgtt ccgcttcttt ggtggtgtgc cgcgcgaagt gttgtatgac aatatgaaaa
      481 ctgtggttct gcaacgtgac gcatatcaga ccggtcagca ccggttccat ccttcgctgt
      541 ggcagttcgg caaggagatg ggcttctctc cccgactgtg tcg
//
LOCUS       DAZSNO010000485          573 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZSNO010000485 DAZSNO010000000
VERSION     DAZSNO010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.495"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(47..573)
                     /locus_tag="ACHWN2_004608"
                     /pseudo
     CDS             complement(47..573)
                     /locus_tag="ACHWN2_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          161 a          135 c          116 g          161 t
ORIGIN      
        1 ccatcttaaa tgactctgag gtgtctgtta aacccgtggc gattcaggtc tgcatagcat
       61 tatcaacatt cggatgtgca actacagcta atgcaacatc atcccagctt gcaatagtgc
      121 tatcagcaac accatctccg tcagtatcta cggtgacaaa gttagattta ccactataat
      181 cgctggcatt acctgtatta ccgaaccaac cagtagtacc ttcatcagtc catgaaccag
      241 aggttacggt agaatcttta acagtgatgg tgttattaaa cacttcgctg ctgtttacgc
      301 cagtgctgac agtgttgtcg tcaatatctt cccactcata accctgagtc aacgtaatac
      361 ccgcaacgtg agagttatta gtgatggaca gatcaacttc ctgatccaga gtaatagccg
      421 taccaatgtt gtaaacgtct acaacatgag tatcagcggc attattgtaa gtgccgttat
      481 atgtgtagtg ttcgtagttg tcgtcgatcg ttgaattatc aacagtcagc gccagacggt
      541 catggtagta atcatccgca cgatcagcac aat
//
LOCUS       DAZSNO010000486          566 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZSNO010000486 DAZSNO010000000
VERSION     DAZSNO010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.496"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..297
                     /gene="pliG"
                     /locus_tag="ACHWN2_004609"
     CDS             <1..297
                     /gene="pliG"
                     /locus_tag="ACHWN2_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR8933779.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="ACHWN2_004610"
     CDS             complement(405..>566)
                     /locus_tag="ACHWN2_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR8933780.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAZSNO010000487          551 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZSNO010000487 DAZSNO010000000
VERSION     DAZSNO010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.498"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>551)
                     /locus_tag="ACHWN2_004611"
     CDS             complement(<1..>551)
                     /locus_tag="ACHWN2_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HFR8933781.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIK"
BASE COUNT          134 a          117 c          135 g          165 t
ORIGIN      
        1 atttaatatc aatctgttga aggtgtatta ttacatcagt agcatattcc agactgcctt
       61 cctggacgat ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa
      121 gtgccgtgag tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct
      181 gactgatcgc ttgggcgacg gtgttttgcc attctaaaga acaggtgctg aagtcaaatg
      241 tggctgtttt attatcccgt tgcgacacgc tacaggctaa gcgacgtaat tgggtcaatt
      301 tgtctgcttg ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa
      361 cttcgggcgg aaaaacgaca gcaatatctt tcgccagcca aggtaaaaca gcatctgtat
      421 ttttgtcagc cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata
      481 agtccgctac cttgagagag tccgttaccg tttgggaagg ttgcaaagaa ttatctttcg
      541 cttgctccac a
//
LOCUS       DAZSNO010000488          550 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZSNO010000488 DAZSNO010000000
VERSION     DAZSNO010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.499"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>550)
                     /gene="ipaH9.8"
                     /locus_tag="ACHWN2_004612"
     CDS             complement(<1..>550)
                     /gene="ipaH9.8"
                     /locus_tag="ACHWN2_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR8933782.1"
                     /translation="RDEAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSY
                     NQLTNLPELPVTLKKLYSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNI
                     SYNEIVSLPSLPQALKNLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILN
                     LRNECSIHISDNPLSSHALQAL"
BASE COUNT          155 a           91 c          137 g          167 t
ORIGIN      
        1 tgcagggctt gcagagcatg ggatgataat gggttatcac taatatgtat tgaacattca
       61 ttcctcagat taagaatgct ttccgggata tgacttatct gatttctatc aaaaaaatac
      121 tctctgacaa cgggattatt tccctcagaa aatgctggta gctcagtgag gaaattacgg
      181 gtcgctctga gatttttaag agcctgtggg agcgatggta aggagactat ttcgttatag
      241 ctgatattca tagtcaataa cgaatcgggt aaagctggca ggttttccag ctcattgtgt
      301 tgtacctgaa gtgactccag cgcaggaggt agcacgggca attctgataa tttattgctg
      361 gcggaatata atttttttag cgtaacaggc agttcaggta ggttagttaa ttgattatat
      421 gatacattga gcagcgttat ctgagctggt aagttgtcag gtagcgagga cagatttaaa
      481 cggtccagtc gaagttcatc ggaattattg ataagacatt ctttaagtcg ggagacagcc
      541 tcatcacgct
//
LOCUS       DAZSNO010000489          534 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZSNO010000489 DAZSNO010000000
VERSION     DAZSNO010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.500"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..421
                     /gene="ospC2"
                     /locus_tag="ACHWN2_004613"
     CDS             <1..421
                     /gene="ospC2"
                     /locus_tag="ACHWN2_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR8933783.1"
                     /translation="ISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYL
                     LSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQ
                     KANSGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          191 a           71 c          108 g          164 t
ORIGIN      
        1 tatatctttg gaggatgcca ttaaagcatc caattatgaa gaaattaaca ataaggtgac
       61 tgataaaaaa atggctcatc aagctcttgc ttattctcta ggcaatgcaa aatctgatat
      121 ggcactttat ttactatcta aatttaattt tacaaaacaa gatatcgcag agatggagaa
      181 aatgaacaac aatatgtatt gtgagctgta tgatgttgag tatttactta gtgaagatag
      241 tgccaactat aaagtgctag aatattttat tagtaatgga ttggttgatg taaacaaaag
      301 atttcaaaaa gcaaatagtg gggacactat gcttgacaat gcaatgaaaa gcaaagattc
      361 aaaaacaatt gattttttat taaaaaatgg agcggtatca ggcaaacgat ttgggaggtg
      421 acctaaaata tatttaggtt gtgccctgaa tctgacgttg tcatttttgt tatattgatc
      481 gatatagggg atggtgcaga ccgttcccct gcaacaacac attgcatgtg gtga
//
LOCUS       DAZSNO010000490          533 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZSNO010000490 DAZSNO010000000
VERSION     DAZSNO010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.501"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            177..252
                     /locus_tag="ACHWN2_004614"
     tRNA            177..252
                     /locus_tag="ACHWN2_004614"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:210..212,aa:Ile,seq:cat)
BASE COUNT          140 a          102 c          136 g          155 t
ORIGIN      
        1 attaacattt gcttgttgac tgcgagtatc gactttgcga atggaggcgg tgtcgggttt
       61 tatgtcgcgt gtaatcacga cccaaggtga atgggaacgc ataaaaatct gttatttaca
      121 tcataagatc atcatgttta ttgcgtggtt tataagtatt gttattgcat acttaaggcc
      181 ctttagctca gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag
      241 caagggccac caaatcatca ttcatgtatc aggaagagtt aagggtacaa ggggtactcg
      301 atggcggtct atatttcatg actaacgcca tttcaaggaa gaagtgcggt gggtggtttc
      361 gaatgagggc tggtgttttg gtacgtggca cgccactgca atgtatttat tcatcagtac
      421 tcattcaaaa gtacctggaa aaaatgcccg ctatgccaag cgggcaaatt gatcctaccc
      481 acgtaatatg gacacaggcc taagcgaggt tctggttttc aaattgttcc gga
//
LOCUS       DAZSNO010000491          531 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZSNO010000491 DAZSNO010000000
VERSION     DAZSNO010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.502"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>531
                     /locus_tag="ACHWN2_004615"
     CDS             <1..>531
                     /locus_tag="ACHWN2_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFR8933784.1"
                     /translation="KYNRLLSQRVAAYASEINRLKALVAKLQRMQFGKSSEKLRAKTE
                     RQILEAQERISALQEEMAETLGEQYDPVLPSPLRQSSARKPLPASLPRETRVIRPEEE
                     CCPACGGELSSLGCDVSEQLELISSAFKVIETQRPKLACCRCDHIVQAPVPSKPIARS
                     YAGAGLLAHVVAGKYAD"
BASE COUNT          129 a          148 c          148 g          106 t
ORIGIN      
        1 aaatacaacc gcctgctctc acagcgggtg gcggcttatg cttccgaaat caaccggctg
       61 aaggcgctgg ttgcgaaact gcagcgcatg cagttcggta aaagctcaga aaaacttcgc
      121 gcaaaaaccg aacggcagat actggaagca caggagcgaa tcagcgcact tcaggaagaa
      181 atggcggaaa cgctgggtga gcaatatgac ccggtactgc catctcccct gcgccagtct
      241 tcagcccgta aaccgttacc tgcctcactt ccccgtgaaa cccgggtcat ccgtccggaa
      301 gaggaatgct gcccggcctg tggtggcgaa ctcagttctc tgggatgtga tgtgtcagag
      361 cagctggagc ttatcagcag cgcctttaag gttatcgaaa cacaacgtcc gaaactggcc
      421 tgttgccggt gtgaccatat cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt
      481 tatgccggag cggggcttct ggcccatgtt gttgctggaa aatatgcaga c
//
LOCUS       DAZSNO010000492          512 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZSNO010000492 DAZSNO010000000
VERSION     DAZSNO010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.503"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>512)
                     /locus_tag="ACHWN2_004616"
     CDS             complement(<1..>512)
                     /locus_tag="ACHWN2_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR8933785.1"
                     /translation="IKELLDYQFSTNEIAAVVAERDIEWWQNRASVLTTPQLASGYFN
                     AGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNT
                     RYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDL
                     LKPVNSNQYR"
BASE COUNT          159 a          101 c           81 g          171 t
ORIGIN      
        1 taccgatact gattgctatt tacaggttta agtaaatctt ctttactcca cggagaagct
       61 gctttggcga tcaaaaaact acgtgaaacc ggataatcag cccactcatg ccagggtttt
      121 gtaggtccca catagtgtat aaacacggtg tcatcattga ctggattatc aactttgtct
      181 tttaattcat agttaatact atatcgggta ttatatttct ccgaaataaa cttcacttta
      241 ccattcaata atacattcag tacatcttga tcaaggtggg tgattttact tacccaatct
      301 gggtcacgca acatttcaat agcttttgac gaaatgttat ttagattcca ctcatcaata
      361 tttatcagta aaaaaccagc attgaaatat ccagaagcta actgtggtgt agttaatacc
      421 gaggctcgat tttgccacca ttcaatatct ctttcagcta caactgcggc aatttcatta
      481 gtagaaaatt gataatctaa gagttcttta at
//
LOCUS       DAZSNO010000493          511 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZSNO010000493 DAZSNO010000000
VERSION     DAZSNO010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.504"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..147)
                     /gene="yhaB"
                     /locus_tag="ACHWN2_004617"
     CDS             complement(<1..147)
                     /gene="yhaB"
                     /locus_tag="ACHWN2_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFR8933786.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKI"
     gene            complement(403..>511)
                     /locus_tag="ACHWN2_004618"
     CDS             complement(403..>511)
                     /locus_tag="ACHWN2_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR8933787.1"
                     /translation="YFKISNTGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          155 a          103 c           97 g          156 t
ORIGIN      
        1 aattttatta acgtcattgg tggagacaat ttctgcaaac tttcttttta ccgtccaata
       61 atcaatattt ctattgtgat tgttaaccac tgcgtgcatt ggcgggattg aagggtgaaa
      121 aatatgtgca attgggaagc ctttcattga tgcctgcctt gtcgtcatga caaacaataa
      181 tagatgctta ttttcctcgc acgacactta cgcactatca aaaaacgctc atttacccgg
      241 cgttacagta aacgaagcaa cattctgtgt cctacgctga tgtaaagcac agtgaaaatc
      301 ccccttgttg acttcgtctg accttatcgc ggcgaccagc aattattcgt tttgtgatcc
      361 tcaacacaaa atgctattcg tcgcctatga ggggagaaga atttatggaa aacgattgca
      421 ttcacaaaat gacgtcctgt cagaagagaa aaagttgacg ttaatcaatt gttcgagatg
      481 atcaatacct gtattcgaaa ttttaaaata t
//
LOCUS       DAZSNO010000494          511 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZSNO010000494 DAZSNO010000000
VERSION     DAZSNO010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.505"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..511
                     /locus_tag="ACHWN2_004619"
                     /pseudo
     CDS             1..511
                     /locus_tag="ACHWN2_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAZSNO010000495          497 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZSNO010000495 DAZSNO010000000
VERSION     DAZSNO010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.506"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..497
                     /locus_tag="ACHWN2_004620"
                     /pseudo
     CDS             1..497
                     /locus_tag="ACHWN2_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
BASE COUNT          113 a          131 c          150 g          103 t
ORIGIN      
        1 gttcacttac accgcttctc aacccggtac gcaccagaaa atcattgata tggccatgaa
       61 tggcgttgga tgccgggcaa ccgcccgcat tatgggcgtt ggcctcaaca cgattttccg
      121 ccatttaaaa aactcaggcc gcagtcggta acctcgcgca tacagccggg cagtgacgtc
      181 atcgtctgcg cggaaatgga cgaacagtgg ggatacgtcg gggctaaatc gcgccagcgc
      241 tggctgtttt acgcgtatga caggctccgg aagacggttg ttgcgcacgt attcggtgaa
      301 cgcactatgg cgacgctggg gcgtcttatg agcctgctgt caccctttga cgtggtgata
      361 tggatgacgg atggctggcc gctgtatgaa tcccgcctga agggaaagct gcacgtaatc
      421 agcaagcgat atacgcagcg aattgagcgg cataacctga atctgaggca gcacctggca
      481 cggctgggac ggaagtc
//
LOCUS       DAZSNO010000496          490 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZSNO010000496 DAZSNO010000000
VERSION     DAZSNO010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.507"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>490)
                     /locus_tag="ACHWN2_004621"
     CDS             complement(<1..>490)
                     /locus_tag="ACHWN2_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933788.1"
                     /translation="YFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFST
                     LFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQN
                     ADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINP
                     LKD"
BASE COUNT          198 a           97 c           77 g          118 t
ORIGIN      
        1 atctttaagt ggattgatag taaatagtag gattgtagca ataatgcctg gataataagt
       61 tttcgtaatt tcccatgaac agccaacaag tccacaggct attacaaaaa aacagatatt
      121 tgctaaaaac tgagctaacc ttacatctcc taactgtaaa cctaaagcca ttaaaagatc
      181 tgcattttgc gggaacagag gaaaacgcaa atattgttca acaacaattg catggtgctc
      241 aactaatgaa cgagccaaag gtaagtgata catagtatca tcccaatcac cgggagctcg
      301 cagggacgat aacaatgtaa ccacaaaaat caaaacaaga aataaaataa caaacagcgt
      361 actgaactca atattattta ttgccagcca tctaaattgc tgacgtttaa taatgcttaa
      421 aacccaacac accgtaaaaa ttaaaaagat agcaatctta ttaaattgcc cgaccatgcc
      481 cagaaagtaa
//
LOCUS       DAZSNO010000497          486 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZSNO010000497 DAZSNO010000000
VERSION     DAZSNO010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.508"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          159 a           73 c           96 g          158 t
ORIGIN      
        1 tttcctgcta aaaatagccc tgctttacgg atggttgtat tgcaataaca cgaaagtagt
       61 aagagattaa aggtatctgt cagcgataac tctgcaagtg atgacatttt gtaaaccaaa
      121 taatgtaaat cagcaatgta tcttttcact taattataac aaatgtggta ttcactaaga
      181 atctgatacg agcttacatc acgactattt tagtctcatc gcgggataca gggatgcacc
      241 gatggatctc agcacaccgt aatgcaatac ctgaacaaat agaacagtaa agtggttcag
      301 tattaatgaa agctcttatt ttccgttggg aaaacttaaa atagatgaga aggctttttg
      361 atttcttatg tgatctcatt tcgagtattg agaaatttgt gatttttaaa tttttcaata
      421 agccctgtaa gggattgggg ttttatgaga aataaagaga cgtattgtct ttgtttaaac
      481 ggtacg
//
LOCUS       DAZSNO010000498          485 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZSNO010000498 DAZSNO010000000
VERSION     DAZSNO010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.509"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..485
                     /locus_tag="ACHWN2_004622"
                     /pseudo
     CDS             1..485
                     /locus_tag="ACHWN2_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          140 a          126 c          124 g           95 t
ORIGIN      
        1 tcagggcaaa acaccaaaag cctcccccat taccccggaa caaattgaaa tccgtgagct
       61 caggaaaaag ctacaacgca ttgaaatgga gaatgaaata ttaaaaaagg ctaccgcgct
      121 cttgatgtca gactccctga acagttctcg ataatcggga aactcagagc gcattatcct
      181 gtggtcacac tctgccatgt gttcggggtt catcgcagca gctacagata ctggaaaaac
      241 cgtcctgaaa aaccagacgg cagacgggct gtattacgca gtcaggtact tgagctacat
      301 ggcatcagcc acggttcggc cggagcaaga agcatcgcca caatggcaac ccggagaggc
      361 taccagatgg gacgctggct tgctggcagg ctcatgaaag agctggggct ggtcagctgt
      421 cagcagccga ctcaccggta taaacgtggt ggtcatgaac atgttgctat ccctaactac
      481 cttga
//
LOCUS       DAZSNO010000499          484 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZSNO010000499 DAZSNO010000000
VERSION     DAZSNO010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.510"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>484
                     /locus_tag="ACHWN2_004623"
     CDS             <1..>484
                     /locus_tag="ACHWN2_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR8933789.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     E"
BASE COUNT          109 a          107 c          123 g          145 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaa
//
LOCUS       DAZSNO010000500          473 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZSNO010000500 DAZSNO010000000
VERSION     DAZSNO010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.511"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
BASE COUNT          148 a           79 c           90 g          156 t
ORIGIN      
        1 gagaaatctg ctaatatatt cttaaagctt ttttgatact gtgaacgaag tctgttaatg
       61 gtggattttt tgtttatcac catgtaaaaa aaattgagta gtaaaagcgt tgactgctaa
      121 tcctcgctgt ttttattgaa acgattaaaa atacgctgaa tattttcggc cagtcactca
      181 tattcacata aaattttgac catcatgccg catcccttag ttgcacaatt cattttcaat
      241 gatatgaata ttactaattt caccagataa ttttctcgtg gtcagcaggg tatgagtttt
      301 caccgtaatt ttaaggaaac acagtgcgtg gggattgaat aacagatagc cgacggaatt
      361 accccaccgg ctgaaagtca agttcaacga agatttttat tatctgaatt gggcagttta
      421 catcagtgtt cgatgtttat gaagacgata gatggcgaga aattatatcc cga
//
LOCUS       DAZSNO010000501          468 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZSNO010000501 DAZSNO010000000
VERSION     DAZSNO010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.512"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>468
                     /locus_tag="ACHWN2_004624"
     CDS             <1..>468
                     /locus_tag="ACHWN2_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR8933790.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASAN"
BASE COUNT          148 a           96 c          103 g          121 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agctaatg
//
LOCUS       DAZSNO010000502          463 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZSNO010000502 DAZSNO010000000
VERSION     DAZSNO010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.513"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..139)
                     /locus_tag="ACHWN2_004625"
     CDS             complement(<1..139)
                     /locus_tag="ACHWN2_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933791.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DV"
     gene            157..348
                     /locus_tag="ACHWN2_004626"
     CDS             157..348
                     /locus_tag="ACHWN2_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR8933792.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          124 a          114 c           80 g          145 t
ORIGIN      
        1 ttacatcatc attgtaaata attaaattaa cttccataac attaaaatat gtatccactg
       61 acgctttttt acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac
      121 cgtccacagc gtgggacata cttcaaggaa ccttttgtga gtcaggcaac tagtatgcga
      181 aaacgacacc gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc
      241 ttctttggcc gttttatcta ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa
      301 gaagctcagc aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct
      361 ccactttcac agaacataac ggcacttcgc tgtcggatgc ttttgctctt tgggattatc
      421 aaagcggcag atattctttc atcttaaatt ttacgtcttt gtc
//
LOCUS       DAZSNO010000503          460 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZSNO010000503 DAZSNO010000000
VERSION     DAZSNO010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.514"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..288)
                     /locus_tag="ACHWN2_004627"
     CDS             complement(<1..288)
                     /locus_tag="ACHWN2_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933793.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMM"
BASE COUNT          135 a          106 c           82 g          137 t
ORIGIN      
        1 catcattgca aactgtgata ctgctgatcc accacctaaa tcaggccacc cagcacaatt
       61 aaaaatgatt gtctgctctt cactattcat tgcatccctg ataaaggaga cagcatcctc
      121 atttctctct acatctgaat cactgttaat ctgagtgaaa tcataatatt tagaacctgg
      181 tatagaatac cccttaaaaa acttatcaat gtgggaggca gagttcccaa caataagaat
      241 attgtgttca cgcttaatca tcaattctga tacatcatca tatttcatat attcaccatt
      301 attatataaa gtttgggtac aggtggttcc ggaatttact ctgtgaccac catttcccgc
      361 tttttattac agaaacgccg ggagcgggga aaacttgctt tttcccgttt ccggggttgg
      421 acaactgagc aacgcgaagg cgtcagctac gatgttccgg
//
LOCUS       DAZSNO010000504          459 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZSNO010000504 DAZSNO010000000
VERSION     DAZSNO010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.515"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..144
                     /locus_tag="ACHWN2_004628"
     CDS             <1..144
                     /locus_tag="ACHWN2_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR8933794.1"
                     /translation="RLAGDPGRESAPDASSVIHATGGDRVATSQTDRTAWHPDITRDK
                     TRE"
     gene            141..>459
                     /locus_tag="ACHWN2_004629"
     CDS             141..>459
                     /locus_tag="ACHWN2_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR8933795.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          137 a          115 c          124 g           83 t
ORIGIN      
        1 cgtctggccg gtgacccggg aagggaaagt gcacctgacg ccagctcagt tatccatgct
       61 actggagggg atcgcgtggc aacatcccaa acggacagaa cggcctggca tccggatata
      121 acccgtgata aaacaaggga atgaacaatg aactccccga tgatattgag ctgcttaaag
      181 ccatgttgcg taagcaacag agtcggcttc gacagtatgc ctgtcaggtc gcgggctatg
      241 agcaggaaat tgaacggctg aaagcgcaac tcgacaggtt gcgtcgtatg ttgttcggcc
      301 agagttcaga gaaaaagcgt cataagcttg aaaatcagat ccgacaggca gaaaaacgac
      361 tgtcggaact ggaaaaccgg ctgaacacag ccagaaatct tctggaagat gcatcgtcag
      421 tcacagattc acctgacacc agtcccccgt cagaaaacc
//
LOCUS       DAZSNO010000505          459 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZSNO010000505 DAZSNO010000000
VERSION     DAZSNO010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.516"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..>459)
                     /locus_tag="ACHWN2_004630"
     CDS             complement(<1..>459)
                     /locus_tag="ACHWN2_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFR8933796.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DAZSNO010000506          452 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZSNO010000506 DAZSNO010000000
VERSION     DAZSNO010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.517"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..>452
                     /gene="virF"
                     /locus_tag="ACHWN2_004631"
     CDS             <1..>452
                     /gene="virF"
                     /locus_tag="ACHWN2_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HFR8933797.1"
                     /translation="EEKRGLNKKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLS
                     AVSDEEALYTSISIASSLSFSDQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLES
                     EKLTFQQILLDIRMHHAAKLLLNSQSYINDVSRLIGISSPSYFIRKFN"
BASE COUNT          155 a           65 c           83 g          149 t
ORIGIN      
        1 tgaggagaaa agggggttaa acaaaaaaat attcctcctc tctgaggagg aggtttctat
       61 cgatttgttc aaatctataa aagagatgcc tttcggcaaa agaaagatct atagtttagc
      121 ttgcctttta tcagctgttt ctgatgagga agctttatat acttcgatat cgatagcttc
      181 ttctcttagt ttttctgatc agataaggaa gattgttgaa aaaaacatcg agaagagatg
      241 gcgtctttct gatatttcaa ataacttgaa tttatcagaa atagctgtta gaaaacgatt
      301 ggagagtgaa aaattaacat ttcaacaaat ccttcttgat attcgcatgc atcatgcagc
      361 aaagctttta ttgaatagtc aaagctatat taatgatgta tcaagactta tcggaatatc
      421 aagcccatct tattttataa ggaaatttaa tg
//
LOCUS       DAZSNO010000507          448 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZSNO010000507 DAZSNO010000000
VERSION     DAZSNO010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.518"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..382)
                     /locus_tag="ACHWN2_004632"
     CDS             complement(<1..382)
                     /locus_tag="ACHWN2_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933798.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLL"
BASE COUNT          135 a          106 c           96 g          111 t
ORIGIN      
        1 ttaacagact tgattcatcc tcacaaaata taagtttttc ctggaatgcc catgtctcat
       61 tgaggttcca gatatcaaat tcgaagtcgt gataacgaaa acggatccca ccaaacttat
      121 taaaacgcag ttgtttgacg ggcagttcag ccaatgtctg gaagagttcc tcacgggagc
      181 gaccgatgac gatatcaagg tctgaagaga atccatcgac gccatacaag cctaagtcac
      241 gcaccatccc gccaaaaatc ctggccggac aggaacaggc cagagtatca agtaatactc
      301 tggctttctt atgctcatca ctaagcgtaa aaaaatcggc gatacgctgt tcaagcagtt
      361 gggtgtttga gaccacattc atacagtgat tttagctcat cgtgaaggcg cattaaataa
      421 ctatccgtca tgcctgagat tgcgtcgg
//
LOCUS       DAZSNO010000508          437 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZSNO010000508 DAZSNO010000000
VERSION     DAZSNO010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.519"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..202)
                     /locus_tag="ACHWN2_004633"
     CDS             complement(<1..202)
                     /locus_tag="ACHWN2_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFR8933799.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYS"
     gene            302..>437
                     /locus_tag="ACHWN2_004634"
     CDS             302..>437
                     /locus_tag="ACHWN2_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR8933800.1"
                     /translation="MRMNMSDFATSFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     L"
BASE COUNT          114 a          104 c           92 g          127 t
ORIGIN      
        1 gagaataacg tttatccata ttcttccagt ctggttcatc ttccgacgtc atcaccgggt
       61 tgaaattacg ccgatataaa tcgagttccg tcacctgtat tttccgctcc attgcccgct
      121 gatggatcgc atcagcaata tttgcggtca atgaatcatg acgaggatgg gcccatacca
      181 aataaatacg ttcagactgc ataattttac ctccacgtta agttctggag cgcactttac
      241 gcaggcggca taatcctgta aacgtgatga ttcaggatga gttaatgaag gattttcact
      301 aatgcggatg aatatgtctg actttgccac ttcctttgcc gtggcccgta atcaaagctt
      361 tcgtgcagca ggcgatgagt taggcttatc ctcgtccgcc attagccata gtattaaaac
      421 actggaacaa cgtctta
//
LOCUS       DAZSNO010000509          434 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZSNO010000509 DAZSNO010000000
VERSION     DAZSNO010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.520"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            35..434
                     /locus_tag="ACHWN2_004635"
                     /pseudo
     CDS             35..434
                     /locus_tag="ACHWN2_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAZSNO010000510          432 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZSNO010000510 DAZSNO010000000
VERSION     DAZSNO010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.521"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            1..432
                     /locus_tag="ACHWN2_004636"
                     /pseudo
     CDS             1..432
                     /locus_tag="ACHWN2_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           96 a          107 c          133 g           96 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag tt
//
LOCUS       DAZSNO010000511          428 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZSNO010000511 DAZSNO010000000
VERSION     DAZSNO010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.522"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..132
                     /locus_tag="ACHWN2_004637"
     CDS             <1..132
                     /locus_tag="ACHWN2_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR8933801.1"
                     /translation="NHAAMNTLLGERVMDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            147..>428
                     /locus_tag="ACHWN2_004638"
     CDS             147..>428
                     /locus_tag="ACHWN2_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR8933802.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT          105 a           89 c          133 g          101 t
ORIGIN      
        1 aaccatgcag cgatgaatac acttctcggc gagcgggtga tggatcgcat ggtcatgaac
       61 ggcgggcgct gggtgaattt taactgggag agctggcgtc cgaatgttag ccattcgagg
      121 gttgttaagt agtttcagga ggatttatgg cgaaaccttt tactcccgaa cagcgggaag
      181 aactgaagac gcgaattgtg gaactcgtgc atcaggacgg tcgggtcacg attcggcagt
      241 tgtccgatga aacaggtatc agtcgtgcgt ctgtcggtcg cttatgcata gaactggtcg
      301 caagtggtga tgtatataat tctggctacg gcttattccc gtctgaacag gctcgcaagg
      361 actggcaaag cgcccgcaaa aaactctcga gagtaaaggt gaggaaaccg gttgttgttg
      421 atccggac
//
LOCUS       DAZSNO010000512          416 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZSNO010000512 DAZSNO010000000
VERSION     DAZSNO010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.523"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..373
                     /gene="istB"
                     /locus_tag="ACHWN2_004639"
     CDS             <1..373
                     /gene="istB"
                     /locus_tag="ACHWN2_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFR8933803.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a           99 c          105 g           91 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtgga
//
LOCUS       DAZSNO010000513          416 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZSNO010000513 DAZSNO010000000
VERSION     DAZSNO010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.524"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..224
                     /locus_tag="ACHWN2_004640"
     CDS             <1..224
                     /locus_tag="ACHWN2_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR8933804.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..>416
                     /locus_tag="ACHWN2_004641"
     CDS             270..>416
                     /locus_tag="ACHWN2_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFR8933805.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLA"
BASE COUNT          128 a           91 c           99 g           98 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcg
//
LOCUS       DAZSNO010000514          416 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZSNO010000514 DAZSNO010000000
VERSION     DAZSNO010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.525"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..127
                     /locus_tag="ACHWN2_004642"
     CDS             <1..127
                     /locus_tag="ACHWN2_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR8933806.1"
                     /translation="VSPSQWAAIGVLGSLLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            127..>416
                     /locus_tag="ACHWN2_004643"
     CDS             127..>416
                     /locus_tag="ACHWN2_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFR8933807.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVARQINPYIKVDIPET"
BASE COUNT          117 a           86 c          109 g          104 t
ORIGIN      
        1 ggtcagtcca tcacagtggg ctgcgattgg tgtgctggga agtctgcttc tggggcttct
       61 gacttatctg acgaatctgt atttcaaaat aagagaagat aagcgtaagg ctgcgagagg
      121 tgaataatgt cgccatcatt acgcaaggct gttgcagttg ctattggtgg cggggctgtt
      181 gctatagcat ctgtgttaat cactggccca ggtggtaacg atggtctgga aggtgtcagc
      241 tacataccat acaaagatat cattggtgta tggactgtat gtcacggaca caccggaaaa
      301 gacatcatgc ctggtaaaac gtataccgaa gcagaatgca aagctctcct gaataaagac
      361 cttgtcactg tcgccagaca aattaacccg tacatcaaag tcgatatacc ggaaac
//
LOCUS       DAZSNO010000515          409 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZSNO010000515 DAZSNO010000000
VERSION     DAZSNO010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.denovo.526"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            155..340
                     /locus_tag="ACHWN2_004644"
                     /pseudo
     CDS             155..340
                     /locus_tag="ACHWN2_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          118 a           97 c          108 g           86 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaagg
//
LOCUS       DAZSNO010000516         9163 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.guided.155, whole genome shotgun sequence.
ACCESSION   DAZSNO010000516 DAZSNO010000000
VERSION     DAZSNO010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.guided.155"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            110..1015
                     /locus_tag="ACHWN2_004645"
     CDS             110..1015
                     /locus_tag="ACHWN2_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR8933808.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..2250
                     /gene="mrx(A)"
                     /locus_tag="ACHWN2_004646"
                     /gene_synonym="mrx"
     CDS             1012..2250
                     /gene="mrx(A)"
                     /locus_tag="ACHWN2_004646"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR8933809.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2250..2834
                     /gene="mphR(A)"
                     /locus_tag="ACHWN2_004647"
     CDS             2250..2834
                     /gene="mphR(A)"
                     /locus_tag="ACHWN2_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR8933810.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3327..4091)
                     /locus_tag="ACHWN2_004648"
     CDS             complement(3327..4091)
                     /locus_tag="ACHWN2_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR8933811.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(4318..4623)
                     /locus_tag="ACHWN2_004649"
     CDS             complement(4318..4623)
                     /locus_tag="ACHWN2_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR8933812.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            complement(4634..5839)
                     /gene="chrA"
                     /locus_tag="ACHWN2_004650"
     CDS             complement(4634..5839)
                     /gene="chrA"
                     /locus_tag="ACHWN2_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR8933813.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            complement(5995..6198)
                     /locus_tag="ACHWN2_004651"
     CDS             complement(5995..6198)
                     /locus_tag="ACHWN2_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR8933814.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            complement(6326..7165)
                     /gene="sul1"
                     /locus_tag="ACHWN2_004652"
     CDS             complement(6326..7165)
                     /gene="sul1"
                     /locus_tag="ACHWN2_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR8933815.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(7159..7506)
                     /locus_tag="ACHWN2_004653"
     CDS             complement(7159..7506)
                     /locus_tag="ACHWN2_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR8933816.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(7712..8500)
                     /locus_tag="ACHWN2_004654"
     CDS             complement(7712..8500)
                     /locus_tag="ACHWN2_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR8933817.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            complement(8631..9104)
                     /gene="dfrA17"
                     /locus_tag="ACHWN2_004655"
     CDS             complement(8631..9104)
                     /gene="dfrA17"
                     /locus_tag="ACHWN2_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR8933818.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
BASE COUNT         1934 a         2707 c         2626 g         1896 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     1861 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     1921 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     1981 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2041 ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg
     2101 tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat cggccgtgtt tgcggggctc
     2161 gtggctctac tctcgctccg tcttcaattt gctacaatgg cggcggcagc cacagcctcc
     2221 gccccaaccc atacagaagg tgaacactga tgccccgccc caagctcaag tccgatgacg
     2281 aggtactcga ggccgccacc gtagtgctga agcgttgcgg tcccatagag ttcacgctca
     2341 gcggagtagc aaaggaggtg gggctctccc gcgcagcgtt aatccagcgc ttcaccaacc
     2401 gcgatacgct gctggtgagg atgatggagc gcggcgtcga gcaggtgcgg cattacctga
     2461 atgcgatacc gataggcgca gggccgcaag ggctctggga atttttgcag gtgctcgttc
     2521 ggagcatgaa cactcgcaac gacttctcgg tgaactatct catctcctgg tacgagctcc
     2581 aggtgccgga gctacgcacg cttgcgatcc agcggaaccg cgcggtggtg gaggggatcc
     2641 gcaagcgact gcccccaggt gctcctgcgg cagctgagtt gctcctgcac tcggtcatcg
     2701 ctggcgcgac gatgcagtgg gccgtcgatc cggatggtga gctagctgat catgtgctgg
     2761 ctcagatcgc tgccatcctg tgtttaatgt ttcccgaaca cgacgatttc caactcctcc
     2821 aggcacatgc gtaaacggag gtgtgcagag tccctgcggc aggcgacgaa cacgaccgtc
     2881 gtcgattagt accggtacgg tcggtggtat cgaagtcttg atcaccactc aggtctacgg
     2941 cttacaaatg gtgaccatcc cgatacttgc gtcagagcac cgggccgatt ctttgacagt
     3001 gaatcactcc cgtaaggttg tgccggtgtg ggtgtcccgg gtcgagacga tactccgcca
     3061 atgcgcccag caaacaacct ggccatcgca ggtggtgggg agcggtgtgg cggatgagtt
     3121 ggacaagttg gtgtagcagc acgagcacgg cgagataaca tcgcaggagt tcgacatgct
     3181 caagagacag ctgattgcga atcgcgatgc agattcataa cccgattgcg ggttggcttc
     3241 actccaccat caccgagcag actagcacgg cgggctctgt tgcaaagatt ggcggcagtc
     3301 agaggtaggc tgtcgctctg cgccgatcag gcggctgctg cgaaatggtg gttgagcatg
     3361 cccatggcct ccgtcagcgc cgagggccca atgccaaaag ctctctccac aaggcgcacc
     3421 tcgcccctga tgccgggctg caggcaccag gggcgagcct gtcctttgcg cagggctcgc
     3481 atgacttcga atcccttgat cgtggcatag gccgtgggga tcgatttgaa accgcgcacc
     3541 ggcttgatca gtatcttgag ctttccgtga tcggcctcga tcacgttatt gagatacttc
     3601 acctgccggt gggccgtctc ccggtccagc tttccttcgc gcttcaattc ggtgatcgct
     3661 gcaccatagc tcggcgcttt gtcggtattg agcgtggcag gcttttccca gtgcttcagg
     3721 cctcgcaggg ccttgcccag gaaccgcttc gctgccttgg cgctgcgggt cggcgacagg
     3781 tagaaatcga tcgtgtcgcc ccgcttgtcg actgcccggt acaggtaggt ccacttgccc
     3841 cgcaccttga cgtaggtttc atccaggcgc cagctcggat caaagccacg ccgccagaac
     3901 cagcgcagcc gcttctccat ctccggggcg tagcactgga cccagcgata gatcgtcgta
     3961 tggtcgaccg aaatgccgcg ttccgccagc atttcctcaa ggtcgcgata gctgatcgga
     4021 tagcgacaat accagcgcac cgcccacagg atcacatcac cctggaaatg gcgccacttg
     4081 aaatccgtca tcgttccgtc cgtccaatct ccgccaagca tgctcaagct tcacgatttt
     4141 tgcaacagag cccacacgag tattgagcat agtcgagatt ggtgcagatc acttctgata
     4201 ttgaactgtc aggagctggc tgcacaacag ccattacgcc caatcaactg gtgcagtcgt
     4261 cttctgaaaa tgacaatcca gttagggtat agctcaacct gacatagaag caaaaactca
     4321 accaccttct accaactctc cgaacagctc cttgaccttt gttttcgcat cagcaagtgc
     4381 agttctgcct tgttcagtga tgtcataaac acgccgttca cgtcgcccgg tgcgttcgtg
     4441 gcgtgaggtc agatagcctt ttttttccag gccgtgcagc atcgggtaca cggtgccagc
     4501 gctcatctcg tagccgtgtc ggcgtagctc ttcgatgatc cccagcccaa agacaggttc
     4561 ctcggctgca tggtgaagga tgtgcaggcg gatcaaaccg ccgtagaggt ctttgtcagt
     4621 cattttttgt gcctcacaga gcgacgctca acagccaccc agctgcaccg ctaccgagga
     4681 caaccagcca cggcgggagc ttccagaaca taagtgcgac aagggcaact aatgccaagc
     4741 cgaagtcttg cggctgaaag atggcgctag tccatacagg ctgatacagc gcggccagca
     4801 gcaagccgac tacagcggca ttgatcccgg ccagcgcagc ttggatgcct gtattgcggc
     4861 gcaaacgctc ccaaaatggc attgatccga cgaccagcaa gaacgagggc gcgaagatag
     4921 ccagcagaca cacaatgccg ccgatccagc ccgacggggc ggtgttcatc gaggcaccaa
     4981 gaaacgcggc gaacgtgaac aaagggccgg gcaccgcttg agctgccccg taccccgcga
     5041 gaaaggattc attgttgacc cagccggagg gcaccacttc ggcttgcagt aatggcagca
     5101 caacgtgacc accgccgaac accagtgatc cgacacgata gaaggaatcc accattgcca
     5161 tggtttgact tggcatcagt tcggccaaca ccggcaggcc aatcagcaag acaaagaaca
     5221 gcgagagcca aagcacgccg gcccggtgac tgaccgtgat aggtaggggg tcatgctcaa
     5281 caactttcgc tggcttgaac aataaccggc ctgcgatgcc tgcgatagca atcacgccaa
     5341 cctgtcccca cgcggacggc acaagtaaaa cgacgcaggt agcaattgcc atgatggtga
     5401 ctcgcagccc atccgtgcat aggttacgcg ccatgcccca tactgcttga gcgaccacgg
     5461 ccacagccac cacttttaag ccatgcaacg cgccctgcga gacgtaatcg ccatagctgg
     5521 agatgccgag cgcaaaaagg atcaaggcta tggcagacgg cagcgtgaag ccagcccaag
     5581 cagccagcgc cccgctgtat ccagcccgag acagtcctac cgctatgccg acctggctgc
     5641 ttgcaggccc tggcaagaac tgacaaagcg cgaccaagtc agcatagctc cgttcggaga
     5701 gccagcgccg ccgtgtgaca aattcggcgc ggaagtagcc caagtgcgca atggggccgc
     5761 caaaagatgt caatccaagc cgcagaaaaa taagaaagac cgaccatggt ctgctgtcat
     5821 cggtagggtt attcgtcata ctttcgcctt catgatctgc aacgagttga tcaataataa
     5881 gcgaaattcg ataacgaaat tcgatataaa tctagaaaaa aatacctcta tgtgtactac
     5941 gcagttttag ctgtggcttt cacaggagca cgcttactta cggcttagcg tgctttattt
     6001 tccgttttct gaggcgatcc ctaggagctc ggatctcagg acgaaggtct ccgcgaatgt
     6061 ccggtcgatc cgcgcgacgt cccaggcggg cgttcccttg gcggacatcc acgccgcagc
     6121 gtcgtgcatc agccgcacaa cctcgtcgat atcacccgag caggcgaccc gaacgttcgg
     6181 aggctcctcg ctgtccattc gctcccctgg cgcggtatga accgccgcct catagtgcag
     6241 tttgatcctg acgagcccag catgtctgcg cccaccttcg cggaacctga ccagggtccg
     6301 ctagcgggcg gccggaaggt gaatgctagg catgatctaa ccctcggtct ctggcgtcgc
     6361 gactgcgaaa tttcgcgagg gtttccgaga aggtgattgc gcttcgcaga tctccaggcg
     6421 cgtgggtgcg gacgtagtca gcgccattgc cgatcgcgtg aagttccgcc gcaaggctcg
     6481 ctggacccag atcctttaca ggaaggccaa cggtggcgcc caagaaggat ttccgcgaca
     6541 ccgagaccaa tagcggaagc cccaacgccg acttcagctt ttgaaggttc gacagcacgt
     6601 gcagcgatgt ttccggtgcg gggctcaaga aaaatcccat ccccggatcg aggatgagcc
     6661 ggtcggcagc gaccccgctc cgtcgcaagg cggaaacccg cgcctcgaag aaccgcacaa
     6721 tctcgtcgag cgcgtcttcg ggtcgaaggt gaccggtgcg ggtggcgatg ccatcccgct
     6781 gcgctgagtg cataaccacc agcctgcagt ccgcctcagc aatatcggga tagagcgcag
     6841 ggtcaggaaa tccttggata tcgttcaggt agcccacgcc gcgcttgagc gcatagcgct
     6901 gggtttccgg ttggaagctg tcgattgaaa cacggtgcat ctgatcggac agggcgtcta
     6961 agagcggcgc aatacgtctg atctcatcgg ccggcgatac aggcctcgcg tccggatggc
     7021 tggcggccgg tccgacatcc acgacgtctg atccgactcg cagcatttcg atcgccgcgg
     7081 tgacagcgcc ggcggggtct agccgccggc tctcatcgaa gaaggagtcc tcggtgagat
     7141 tcagaatgcc gaacaccgtc accatggcgt cggcctccgc agcgacttcc acgatgggga
     7201 tcgggcgagc aaaaaggcag caattatgag ccccatacct acaaagcccc acgcatcaag
     7261 cttttgccca tgaagcaacc aggcaatggc tgtaattatg acgacgccga gtcccgacca
     7321 gactgcataa gcaacaccga cagggatgga tttcagaacc agagaaagaa aataaaatgc
     7381 gatgccataa ccgattatga caacggcgga aggggcaagc ttagtaaagc cctcgctaga
     7441 ttttaatgcg gatgttgcga ttacttcgcc aactattgcg ataacaagaa aaagccagcc
     7501 tttcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa
     7561 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcata
     7621 gttgagcggc gggcgcagcc cgtccgcttg aacgccgagt taggcatcag atgccctcgg
     7681 cgcgggtcga tgcacttttc gcacatgccg ctcaacgcaa gattctctca atcgttgctt
     7741 tggcatatcg aacgaacgcg gccgtctctt cgacgcgcat tgctaggtcg tcgtcctcgc
     7801 tacccaggta cgccgcgcgt gccttgcaga tgaggggccg atgctcggca ggcaaacgct
     7861 ccgataccca tgcggcagca acgtccttag gagcaatgag accagttgaa gcgctgtacc
     7921 aaatgcgagc aagagcaaga acgacgttcc gctcgtcacc cttccaatcc gactctgcat
     7981 tccactgggc aatagtgtcg aaaagcgcct tggagaaatg ctccttcggc accggctcga
     8041 aaaacgtggc tgcggatggg cctagaagcg caaggctgtg ttgcctcgcc ttggtcagca
     8101 aaatcgcaag atcgtgatcc agaacggcag gctcgaacgt tccggaaagg atgtcgtggc
     8161 ggagccactc accgaactga agctcacgcc gcgccggata gcgccaaggc actacttcgc
     8221 ttcgagcgac aacagttagc tccagcggtc gccatgttcc gccatcgcct ggcggtgatg
     8281 agactttcag caaatcgagc attagcgcct gccggagcga atcgttaggt gcggcgctga
     8341 cggtcacgag caagtctatg tcgctgtccg gcttcagccc tccatcgatc gcagatccga
     8401 acaggtggat tgtgtccagt gtcgcagcca gatggcgctc gatcaccgcg cgagcgtggg
     8461 acagctgctt gaaaacttgt gcagggaaaa attcacccat gatgcctaac gttaagttca
     8521 gcggcagctt ttaagttgcg gctttgtgga atacttttgc gcagcaaaac cacaaagacg
     8581 cgacttaaaa gctgtccaag gagcgaagcg actggtgctg caacgcattg ttagcctttt
     8641 ttccaaatct ggtatgtata atttatatta gacataaaaa actgttcaaa aaccaaattg
     8701 aaattctcag gcattatagg gaatttgata tcaccttcga cttcaacgtg aacagtagac
     8761 aaatgaatta tatctgcttt ttcaataagg ctattataga tttgaccccc gccagagaca
     8821 tatacatgat ctgtaacttt tgatagctct ttcaaagcat tttctattga aggaaaaact
     8881 aggacgtttt catttgagct tgaaattccg ttctttgaca ctactgcata tttgcgattt
     8941 ggaagaacac ccatagagtc aaatgttttt cttccgacaa ggagccattg attatatgtg
     9001 agcgctttaa agagtagttg ctcacctttt actgaccacg ggatatcagg accactaccg
     9061 attacgccat tttctgacac tgcagaaatc aatgatattt tcaatttaac tcccttaatg
     9121 gctaactttg ttttagggcg actgccctgc tgcgtaacat cgt
//
LOCUS       DAZSNO010000517         8872 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.guided.158, whole genome shotgun sequence.
ACCESSION   DAZSNO010000517 DAZSNO010000000
VERSION     DAZSNO010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.guided.158"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(35..205)
                     /locus_tag="ACHWN2_004656"
                     /pseudo
     CDS             complement(35..205)
                     /locus_tag="ACHWN2_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(424..1401)
                     /gene="epmA"
                     /locus_tag="ACHWN2_004657"
     CDS             complement(424..1401)
                     /gene="epmA"
                     /locus_tag="ACHWN2_004657"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR8933819.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1726..3534
                     /gene="frdA"
                     /locus_tag="ACHWN2_004658"
     CDS             1726..3534
                     /gene="frdA"
                     /locus_tag="ACHWN2_004658"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR8933820.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3527..4261
                     /gene="frdB"
                     /locus_tag="ACHWN2_004659"
     CDS             3527..4261
                     /gene="frdB"
                     /locus_tag="ACHWN2_004659"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR8933821.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4272..4667
                     /gene="frdC"
                     /locus_tag="ACHWN2_004660"
     CDS             4272..4667
                     /gene="frdC"
                     /locus_tag="ACHWN2_004660"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR8933822.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4678..5037
                     /gene="frdD"
                     /locus_tag="ACHWN2_004661"
     CDS             4678..5037
                     /gene="frdD"
                     /locus_tag="ACHWN2_004661"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR8933823.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5100..6233
                     /locus_tag="ACHWN2_004662"
     CDS             5100..6233
                     /locus_tag="ACHWN2_004662"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR8933824.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6322..6855
                     /gene="blc"
                     /locus_tag="ACHWN2_004663"
     CDS             6322..6855
                     /gene="blc"
                     /locus_tag="ACHWN2_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR8933825.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6852..7169)
                     /gene="sugE"
                     /locus_tag="ACHWN2_004664"
     CDS             complement(6852..7169)
                     /gene="sugE"
                     /locus_tag="ACHWN2_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR8933826.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7345..7491)
                     /gene="ecnB"
                     /locus_tag="ACHWN2_004665"
     CDS             complement(7345..7491)
                     /gene="ecnB"
                     /locus_tag="ACHWN2_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR8933827.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7602..7727)
                     /gene="ecnA"
                     /locus_tag="ACHWN2_004666"
     CDS             complement(7602..7727)
                     /gene="ecnA"
                     /locus_tag="ACHWN2_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR8933828.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7779..8345)
                     /gene="efp"
                     /locus_tag="ACHWN2_004667"
     CDS             complement(7779..8345)
                     /gene="efp"
                     /locus_tag="ACHWN2_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR8933829.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8387..>8872
                     /locus_tag="ACHWN2_004668"
     CDS             8387..>8872
                     /locus_tag="ACHWN2_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HFR8933830.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IF"
BASE COUNT         2255 a         2289 c         2259 g         2069 t
ORIGIN      
        1 tgaacataaa acactatcag taagttggag tcattaccat taaggcgaat ggaataaaga
       61 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
      121 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
      181 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
      241 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
      301 atacttggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
      361 catatgctgc agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      421 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
      481 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      541 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      601 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc
      661 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
      721 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
      781 ttctttacca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
      841 gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgcgactt cccgcagttg
      901 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
      961 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     1021 caaccggtac atatcatagt gcggtcgata ccactccagc atagtgaact cagggttgtg
     1081 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     1141 agcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     1201 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1261 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
     1321 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     1381 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1441 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     1501 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1561 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1621 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     1681 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     1741 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     1801 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     1861 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     1921 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     1981 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtggggatg cccatggagc
     2041 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     2101 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     2161 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     2221 ggtcatgttc gcggcctggt agcaataaac atgatggaag gcacgttggt gcagatccgt
     2281 gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg tttatcgtta caacaccaac
     2341 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2401 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
     2461 gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2521 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2581 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     2641 tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc acctcggcga gaaaaaactg
     2701 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     2761 aaagaaccga ttccggtacg tccgactgca cactacacca tgggcggtat cgaaaccgat
     2821 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     2881 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     2941 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
     3001 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctagttaa ccaggatggc
     3061 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga aggttgcggt
     3121 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     3181 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
     3241 accattgaac tgagccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3301 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
     3361 gtcaactttc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
     3421 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
     3481 gatgcagccg ataaggcgga agcagccaat aataaggaga aggcgaatgg ctgagatgaa
     3541 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3601 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     3661 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
     3721 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     3781 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
     3841 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     3901 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     3961 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     4021 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     4081 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggtg tatggagctg
     4141 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     4201 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4261 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4321 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4381 tgtggttcag cattgaactg attttcgcgc tgtttgccct gaaaaatggc ccggaagcct
     4441 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     4501 tgacggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4561 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4621 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     4681 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     4741 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     4801 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     4861 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     4921 cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc ctgcgggcaa atgggttttc
     4981 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac aatctaacgc
     5041 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     5101 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttactccaca tttgctgccc
     5161 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     5221 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     5281 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5341 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     5401 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     5461 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg ccggatgaag
     5521 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5581 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     5641 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     5701 accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg ggatatcgcg
     5761 aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     5821 cgaccattga agatatggcc cgctgggtgc aaagcaattt accccccctt gatatcaatg
     5881 agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
     5941 tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta
     6001 acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa
     6061 ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct
     6121 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
     6181 ccaatccagc gagagtcgcc gccgcctgtc agattctcaa cgctctacag taaaatttca
     6241 tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa
     6301 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
     6361 tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg gcgtgaccgt agtgaataat
     6421 ttcgacgcca aacgttatct cggtacctgg tatgagattg cccgttttga tcaccgtttt
     6481 gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat
     6541 gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga agggaaagcg
     6601 tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat
     6661 ggcggttata acgttattgc actcgatcgg gaataccgcc atgcgctggt ttgcggcccg
     6721 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
     6781 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
     6841 cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
     6901 agactcgcca gacgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc
     6961 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctaccgg taacgatttc
     7021 atcgcccagg caagtagcgc catactgact atcatcgccg tcacggtaat aacactcggc
     7081 gtcaaacggc taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga
     7141 ccagcaataa ctaagataat ccaggacata tcaggctccg gaaatttggg gccgtccccg
     7201 gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
     7261 ggtggcaggg gaatttcaac tattaccttt atgacgatcg cagaaaaaac aaacggcacg
     7321 acactgctgt cgtaccgtta ttgcttattg ctgcgctttc gttgctgcgc cagaaatcgc
     7381 gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg cagttaatac
     7441 tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc
     7501 cttttaggta atgttattta tgtttgccta tagcaaatta ataatagaca acatcgccaa
     7561 aaatgatgga aatctgatga tttttcggaa gagaagaaaa attagctggc agcgcgagag
     7621 atggagttac cgagatgttt gatgtcttcg ccgaaaccgc aagcggtgtt acagcccgtg
     7681 agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc tgacctgaat
     7741 aagtgatggt gcagcctgca ggccgcacca caaccgcatt acttcacgcg agagacgtat
     7801 tcaccagagc gggtatccac tttgatgact tcgccgattt gtacgaacag cggaacttta
     7861 accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt atcacctttc
     7921 aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt aacggagatc
     7981 ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcagcca tttagcattg
     8041 tcgccaattg cttttgcatc agcagacagc tgctcgaaag tttcgttgtt catgaagtgc
     8101 cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc agcgccttca
     8161 gcggaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag acgacgcagt
     8221 ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc ttcaaccgcg
     8281 taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct atagtacgtt
     8341 gccataaggc cctctgaaat ttgttaattg gtagctaagc cacaaaatgg cgcatattgt
     8401 aaccctaaat accccatcca gagaagattg gttaacgcaa cttgccgatg ttgtgaccga
     8461 tcctgatgaa cttctgcgtc ttttgaatat agacgcggac gaaaaactgt tggccggacg
     8521 cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca tttatcgatc gcatggagaa
     8581 aggcaatccg gacgatcctc ttttgcgtca ggtacttacc tcgcaagatg agtttgtcgt
     8641 cgcgtccgga ttctccaccg acccactgga agaacagcac agcgtagtgc ctggtttgtt
     8701 gcataaatac cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg
     8761 ctattgcttc cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc gtaactggca
     8821 gactgcactt gagtatgttg ctgcgcatcc ggaactggac gagatgattt tc
//
LOCUS       DAZSNO010000518         4395 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.guided.237, whole genome shotgun sequence.
ACCESSION   DAZSNO010000518 DAZSNO010000000
VERSION     DAZSNO010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.guided.237"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            complement(<1..634)
                     /locus_tag="ACHWN2_004669"
     CDS             complement(<1..634)
                     /locus_tag="ACHWN2_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFR8933831.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGL"
     gene            896..1354
                     /gene="gpt"
                     /locus_tag="ACHWN2_004670"
     CDS             896..1354
                     /gene="gpt"
                     /locus_tag="ACHWN2_004670"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR8933832.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1446..2690
                     /gene="frsA"
                     /locus_tag="ACHWN2_004671"
     CDS             1446..2690
                     /gene="frsA"
                     /locus_tag="ACHWN2_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR8933833.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2748..3149
                     /gene="crl"
                     /locus_tag="ACHWN2_004672"
     CDS             2748..3149
                     /gene="crl"
                     /locus_tag="ACHWN2_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR8933834.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(3188..4243)
                     /gene="phoE"
                     /locus_tag="ACHWN2_004673"
     CDS             complement(3188..4243)
                     /gene="phoE"
                     /locus_tag="ACHWN2_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR8933835.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1091 a         1073 c         1075 g         1156 t
ORIGIN      
        1 tcagaccttt taaggttaac ttgaaggttt caaaaccagc tggaaccgct tcacgatcta
       61 aatgcaggtt ggaggtgaag tcgatacccc ccgcacaacc catgtagatt tcaccttctt
      121 cttcggagtc ggtgttaatc agaatatcag cctgcaacca gttgctctgt aagccgaacg
      181 caccgtccat accggcttct tcggtcatgg tcagcagcac ttccagcggg ccgtgaacca
      241 cgttttcgtc agccagaacc gccagtgcag aggccatacc aatgccgtta tccgcaccca
      301 gcgtggtgcc gcgcgcttta acccattcgc catcaatata aggctggata ggatctttcg
      361 tgaagtcatg cacggtgtcg ttatttttct gcggcaccat atcgaggtgg gcctgtaaga
      421 cgaccggttt acgattttcc atacctgcgg tagcaggttg acgaatcagg atattaccta
      481 cctgatcgcg ttcgacatgg aaacctttct cttttgccca accaacaatg tattcagcga
      541 gttgctcttc atgataggac gggtgaggaa tagaacagat tttggcaaaa atatcccaca
      601 gcggctgtgg agataattga gacagttcag acacgttaag tctccttgtc gatcacccgc
      661 aaaacagtat tgcaggtcac agggttagca gaaaatgttg tcaacacaag acaggcttgc
      721 gagatatgta ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
      781 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
      841 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
      901 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
      961 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctggtacc
     1021 gggggcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     1081 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     1141 aggcttcatc gttattgatg acctggtgga taccggtggt actgctgttg cgattcgtga
     1201 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     1261 tgatgactat gttgttgata tcccgcaaga tacctggatc gaacagccgt gggatatggg
     1321 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cattgccggg
     1381 cgttgttctt tttaacttca ggcaggttac aatagtttcc agtaagtatt ctggaggctg
     1441 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     1501 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggctc
     1561 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
     1621 gcggtattga cccacgtgaa atcctcgacg tccaggcacg tattgtgatg agcgatgctg
     1681 aacgtaccga cgatgattta tacgataccg tgattggcta ccgtggtggc aactggattt
     1741 atgagtgggc cacccaggcg atggtgtggc aacaaaaagc ctgtacggaa gaagatccgc
     1801 aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta caacattgcc gcctatcctc
     1861 atctgaaagg agatgacctg gccgagcaag cgcaggcttt atcaaaccgc gcctatgaag
     1921 aggccgccca gcgtctgccg ggcacgatgc ggcagatgga gtttaccgta cccggcggtg
     1981 cgcccatcac cggctttttg catatgccga aaggcgatgg cccgttcccg acagtattaa
     2041 tgtgtggtgg tctggatgcg atgcagacgg actattacag cctgtatgaa cgttattttg
     2101 cgccgcgcgg cattgcgatg ctgactattg atatgccgtc ggtgggcttt tcttcaaaat
     2161 ggaagctcac ccaggactcc agcctgttgc atcagcacgt cttaaaggcg ctgcctaacg
     2221 taccgtgggt ggatcacact cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg
     2281 ccgtgcgtct ggcatacctt gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg
     2341 tagttcatac cctgttgagt gattttaagt gccagcaaca ggtgccggaa atgtatcttg
     2401 acgttctggc gagtcgtttg gggatgcatg atgcttccga cgaagcgtta cgcgtggagc
     2461 tgaatcgcta ttcattaaaa gtgcaaggat tgctggggcg tcgctgccca acgccaatgt
     2521 tatcaggcta ctggaagaac gatccgttca gcccggaaga ggactcacgc ttaatcacct
     2581 catcatctgc tgacggtaaa ttattagaga tcccatttaa cccggtgtat cggaattttg
     2641 acaaaggtct tcaggaaatc accggctgga tcgaaaaacg cttgtgttaa aaatttgcta
     2701 aattttgcca atttggtaaa acagttgcat cacaacagga gatagcaatg acgttaccga
     2761 gtggacaccc gaagagcaga ttgatcaaaa aatttaccgc actaggcccg tatattcgtg
     2821 aaggtaagtg cgaagataat cgattctttt tcgattgtct ggctgtatgc gtcaacgtga
     2881 aaccggcacc ggaagtgcgt gaattctggg gctggtggat ggagctggaa gcgcaggaat
     2941 cccgttttac ctacagctac cagtttggtc tgttcgataa agcaggcgac tggaagagtg
     3001 ttccggtaaa agatactgaa gtggttgaac gactggagca caccctgcgt gagtttcacg
     3061 agaagctgcg tgaactgctg acgacgctga atctgaagct ggaaccggcg aatgattttc
     3121 gtgatgaacc ggtgaagtta acggcgtgag tgaaatgtgc cggatgcatc acatccggca
     3181 atattcatta aaactgatac gtcatcccaa ccgcgacagt atcatcatta ttaatattca
     3241 gcttattatc gctatccagt tggttgattt tataatcaac aaacgctgac atatttttgt
     3301 tgaaataata cgtagcaccg acgtcgatat aattgaccag atcttcatca ccgatgccct
     3361 caatatcttt ccctttcgat aagacataac ccagcgatgg acgcagacca aagtcaaact
     3421 ggtattgagc gaccgcttca aagttctgtg tcttattggc aaagccgcca gttattggcg
     3481 tcattttgcg tgtttcagaa tagaaagttg ccagataaat attattggca tcgtatttca
     3541 gacctgttgc ccatgcttct gcacgcttgc ctgtgccacg gctttgcagg ttctgctcgt
     3601 tggtgcgatc tgagttggta taggccccac taatggcgaa atcgctgccg ccaaagtcat
     3661 atgtcaatga cgtgccgaag ccatcgccgt tttgcttttt aacgtcgcgg ttttcgtttt
     3721 tcccttgata ttgcagggtt aagttcaggc catcgataac gccgaagaag tcggtgttcc
     3781 gatacgtcgc cagaccgctg gcgcgtttgg tcataaagtt gtcggtctgc gccgaggaat
     3841 cgccaccaaa ttccgggaac atatcggtcc aggcttccac gtcatacaac gcgcccaggt
     3901 tacgaccata atcgaaagaa cccaaatctt tatatttcaa cccggcaaaa gcgagacgcg
     3961 ttttttgctg tgcagtatca ctctccgctt tatttccggc aaactccgct tcccaccgac
     4021 cataaccagt cagttgatcg ttaatttgag tttcaccttt aaaaccaaaa cggatataac
     4081 tctggtcgcc atctttactg gcgttatcac tcatataatg catggcttta actttgccat
     4141 agacatccag tttattaccg tctttattat atatttctgc agcctgtaca gatgcagatg
     4201 ccacaatgcc catcaccact aatgccagag tgctcttttt cattttcatt cctgatttta
     4261 attaacgcgc gaatattcag cgggagagcc ccgttgaaaa caggaaagtt tttaacctga
     4321 gattgttaaa gatatattac agattaatga tattctgaaa atgggtaatg ctgccaactt
     4381 actgatttag tgtat
//
LOCUS       DAZSNO010000519         3334 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.guided.272, whole genome shotgun sequence.
ACCESSION   DAZSNO010000519 DAZSNO010000000
VERSION     DAZSNO010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.guided.272"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            20..283
                     /locus_tag="ACHWN2_004674"
     CDS             20..283
                     /locus_tag="ACHWN2_004674"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFR8933836.1"
                     /translation="MSWSHYRPLLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVL
                     QWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            276..662
                     /locus_tag="ACHWN2_004675"
     CDS             276..662
                     /locus_tag="ACHWN2_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HFR8933837.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            670..1356
                     /locus_tag="ACHWN2_004676"
     CDS             670..1356
                     /locus_tag="ACHWN2_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR8933838.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1334..1957)
                     /gene="tetR(B)"
                     /locus_tag="ACHWN2_004677"
     CDS             complement(1334..1957)
                     /gene="tetR(B)"
                     /locus_tag="ACHWN2_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR8933839.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2039..3244
                     /gene="tet(B)"
                     /locus_tag="ACHWN2_004678"
     CDS             2039..3244
                     /gene="tet(B)"
                     /locus_tag="ACHWN2_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR8933840.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          915 a          615 c          742 g         1062 t
ORIGIN      
        1 tgaacataaa acactatcaa taagttggag tcattaccga ccactattaa gtcatgtagc
       61 tggttttatt tcaattgaac gttttcaaag tctagctaca gaaggaaaaa tgttatcgct
      121 atcttggtgg gaaaacgaat acgcagttct gcaatggaaa aatcatgttt tacatgcgaa
      181 agctcaacaa gaagggcgag agtcaatatt tgatttttac aaaattagta ttgctcatat
      241 tactcgcgaa tattcattta aaaaggacaa ggataatgtt tgatgttcac gttgttttag
      301 ataatcaaat aggacaatta gcattactag gaaaaacatt aggtaataaa ggtattggat
      361 tggaaggggg agggatattt acggttggtg atgaatgcca tgctcatttt cttgttgaac
      421 aaggaaagga agctaaaata gcgctagagc aagctggact gttagtactt gcgatccgga
      481 caccattaat tcgtaagtta aaacaggaaa aaccggggga acttggcgaa atagcacgag
      541 tattggcgga gaataacatt aatattttag tgcaatacag tgaccatgct aaccaactga
      601 tattaataac ggacaatgat agtatggctg catctgttac gctcccttgg gcaataaagt
      661 gaacttgcga tggctaattt aatacgaaaa gaggttacct ttgagtcctc aatagccgcg
      721 ataggggcgg ctatgtctga catttcacga gttaaaatac tcagtgcttt gatggatggg
      781 cgagcttgga cggccactga gctaagttct gtggcgaata tatcagcttc aacggcgagc
      841 agtcatttat ctaaattatt agattgccag ctaatcacag tagtagctca aggcaagcat
      901 cgttattttc ggctagcagg aaaagatatt gctgaattga tggaaagtat gatggggatc
      961 tccttaaacc atggcgtaca tgccaaagtt tccacgccag tgcatttacg aaaagcacgt
     1021 acttgctatg atcatttagc tggcgaagtt gccgttaaga tctatgattc cctttgtcaa
     1081 cagcaatgga tcactgaaaa tggttcaatg atcacattaa gtggtattca atattttcat
     1141 gaaatgggaa ttgacgttcc ttccaaacat tcacgtaaaa tctgttgtgc gtgtttagat
     1201 tggagtgaac gccgtttcca tttaggtggg tacgttggag ccgcattatt ttcgctttat
     1261 gaatctaaag ggtggttaac tcgacatctt ggttaccgtg aagttaccat cacggaaaaa
     1321 ggttatgctg cttttaagac ccactttcac atttaagttg tttttctaat ccgcatatga
     1381 tcaattcaag gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag
     1441 cttgtcgtaa taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga
     1501 cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg
     1561 catataatgc attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga
     1621 gagtttcata ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat
     1681 gacttagtaa agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt
     1741 ccccttctaa agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt
     1801 cgagcaaagc ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct
     1861 tctgggcgag tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg
     1921 cgctgttaat cactttactt ttatctaatc tagacatcat taattcctaa tttttgttga
     1981 cactctatca ttgatagagt tattttacca ctccctatca gtgatagaga aaagtgaaat
     2041 gaatagttcg acaaagatcg cattggtaat tacgttactc gatgccatgg ggattggcct
     2101 tatcatgcca gtcttgccaa cgttattacg tgaatttatt gcttcggaag atatcgctaa
     2161 ccactttggc gtattgcttg cactttatgc gttaatgcag gttatctttg ctccttggct
     2221 tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg ttgttgtcat taataggcgc
     2281 atcgctggat tacttattgc tggctttttc aagtgcgctt tggatgctgt atttaggccg
     2341 tttgctttca gggatcacag gagctactgg ggctgtcgcg gcatcggtca ttgccgatac
     2401 cacctcagct tctcaacgcg tgaagtggtt cggttggtta ggggcaagtt ttgggcttgg
     2461 tttaatagcg gggcctatta ttggtggttt tgcaggagag atttcaccgc atagtccctt
     2521 ttttatcgct gcgttgctaa atattgtcgc tttccttgtg gttatgtttt ggttccgtga
     2581 aaccaaaaat acacgtgata atacagatac cgaagtaggg gttgagacgc aatcgaattc
     2641 ggtatacatc actttattta aaacgatgcc cattttgttg attatttatt tttcagcgca
     2701 attgataggc caaattcccg caacggtgtg ggtgctattt accgaaaatc gttttggatg
     2761 gaatagcatg atggttggct tttcattagc gggtcttggt cttttacact cagtattcca
     2821 agcctttgtg gcaggaagaa tagccactaa atggggcgaa aaaacggcag tactgctcgg
     2881 atttattgca gatagtagtg catttgcctt tttagcgttt atatctgaag gttggttagt
     2941 tttccctgtt ttaattttat tggctggtgg tgggatcgct ttacctgcat tacagggagt
     3001 gatgtctatc caaacaaaga gtcatcagca aggtgcttta cagggattat tggtgagcct
     3061 taccaatgca accggtgtta ttggcccatt actgtttgct gttatttata atcattcact
     3121 accaatttgg gatggctgga tttggattat tggtttagcg ttttactgta ttattatcct
     3181 gctatcgatg accttcatgt taacccctca agctcagggg agtaaacagg agacaagtgc
     3241 ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata taatgaccct cttgataacc
     3301 caagagggca ttttttacga taaagaagat ttag
//
LOCUS       DAZSNO010000520         1962 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.guided.341, whole genome shotgun sequence.
ACCESSION   DAZSNO010000520 DAZSNO010000000
VERSION     DAZSNO010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.guided.341"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            109..939
                     /locus_tag="ACHWN2_004679"
     CDS             109..939
                     /locus_tag="ACHWN2_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HFR8933841.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1052..1890
                     /gene="aadA1"
                     /locus_tag="ACHWN2_004680"
                     /pseudo
     CDS             1052..1890
                     /gene="aadA1"
                     /locus_tag="ACHWN2_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          614 a          433 c          435 g          480 t
ORIGIN      
        1 acgatgttac gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat
       61 taattgttag ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca
      121 atacatatca acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt
      181 gcatcaacag atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt
      241 ttactttacg atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca
      301 acgcaaatgg caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg
      361 gaaataatag atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc
      421 tggaacagca atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa
      481 gaaataaccc aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat
      541 ggaaatcaag acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc
      601 gaaagtagct taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat
      661 cacaatctcc cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa
      721 gatctggata atagtacaaa actgtatggg aaaactggtg caggattcac agcaaataga
      781 accttacaaa acggatggtt tgaagggttt attataagca aatcaggaca taaatatgtt
      841 tttgtgtccg cacttacagg aaacttgggg tcgaatttaa catcaagcat aaaagccaag
      901 aaaaatgcga tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc
      961 ccaacccttc aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc
     1021 tcggcgcccc ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc
     1081 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     1141 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     1201 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     1261 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     1321 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     1381 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     1441 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     1501 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     1561 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     1621 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     1681 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     1741 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     1801 tcactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc
     1861 aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat
     1921 tgcggtgatg ctgccaactt actgatttag tgtatgatgg tg
//
LOCUS       DAZSNO010000521         1709 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.guided.365, whole genome shotgun sequence.
ACCESSION   DAZSNO010000521 DAZSNO010000000
VERSION     DAZSNO010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.guided.365"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            276..1136
                     /locus_tag="ACHWN2_004681"
     CDS             276..1136
                     /locus_tag="ACHWN2_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR8933842.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            1283..1564
                     /locus_tag="ACHWN2_004682"
                     /pseudo
     CDS             1283..1564
                     /locus_tag="ACHWN2_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
BASE COUNT          428 a          376 c          439 g          466 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc gtattgcccg ctccacggtt
       61 tataaaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      121 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      181 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      241 ggtaaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat tttcgtgtcg
      301 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      361 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      421 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      481 cttttaaagt tctgctatgt ggtgcggtat tatcccgtgt tgacgccggg caagagcaac
      541 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      601 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      661 ataacactgc tgccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
      721 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
      781 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
      841 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
      901 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
      961 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1021 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1081 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1141 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1201 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1261 cgttccactg agcgtcagac cccttatggc agagcaggga aaggaattgc cgggctatgt
     1321 gcaacgggaa tttgaagaat ttctccaatg cgggcggctg gagcatggct ttctacgggt
     1381 tcgctgcgag tcttgccacg ccgagcacct ggtcgctttc agctgtaagc gtcgcggttt
     1441 ctgcccgagc tgtggggcgc ggcggatggc cgaaagtgcc gccttgctgg ttgatgaagt
     1501 actgcctgaa caacccatgc gtcagtgggt gttgagcttc ccgtttcagc tgcgtttcct
     1561 gtttggggtc gtttgcggga aggggcggaa tcctacgcta aggctttggc cagcgatatt
     1621 ctccggtgag attgatgtgt tcccagggga taggagaagt cgcttgatat ggcactgttg
     1681 caaatagtcg gtggtgataa acttatcat
//
LOCUS       DAZSNO010000522         1052 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.guided.415, whole genome shotgun sequence.
ACCESSION   DAZSNO010000522 DAZSNO010000000
VERSION     DAZSNO010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.guided.415"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            264..923
                     /locus_tag="ACHWN2_004683"
     CDS             264..923
                     /locus_tag="ACHWN2_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR8933843.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          291 a          227 c          245 g          289 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg tt
//
LOCUS       DAZSNO010000523          988 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri 2a isolate 17-00PHL-SF21
            SAMN39618801-rid23472813.guided.417, whole genome shotgun sequence.
ACCESSION   DAZSNO010000523 DAZSNO010000000
VERSION     DAZSNO010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN39618801
            Sequence Read Archive: SRR27734368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002394680.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002394680.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 15:18:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,594
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 477
            CDSs (without protein)            :: 477
            Pseudo Genes (ambiguous residues) :: 0 of 477
            Pseudo Genes (frameshifted)       :: 227 of 477
            Pseudo Genes (incomplete)         :: 160 of 477
            Pseudo Genes (internal stop)      :: 162 of 477
            Pseudo Genes (multiple problems)  :: 67 of 477
            Pseudo Genes (short protein)      :: 1 of 477
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN39618801-rid23472813.guided.417"
                     /isolate="17-00PHL-SF21"
                     /isolation_source="Stool = Fecal"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Canada: Ontario"
     gene            <1..624
                     /gene="erm"
                     /locus_tag="ACHWN2_004684"
     CDS             <1..624
                     /gene="erm"
                     /locus_tag="ACHWN2_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HFR8933844.1"
                     /translation="GKGHLTTKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQ
                     DILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRT
                     LGLLLHTQVSIQQLLKLPAECFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWV
                     NREYRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          346 a          171 c          170 g          301 t
ORIGIN      
        1 ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa
       61 ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaatac tcgtgtcact
      121 ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt
      181 gggaatattc cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccat
      241 gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac
      301 cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca
      361 gcggaatgct ttcatcctaa accaagagta aacagtgtct taataaaact tacccgccat
      421 accacagatg ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc
      481 aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc
      541 aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt taatagttat
      601 ctattattta acgggaggaa ataattctat gagtcgcttt tgtaaatttg gaaagttaca
      661 cgttactaaa gggaatgtag ataaattatt aggtatacta ctgacagctt ccaaggagct
      721 aaagaggtcc ctagactagc aagaagtaca caagaagcct taaagattat agaaaagctt
      781 ttccatgaaa tgcttaacat tatttaatgt taacatgtgg ctttgtggta actaccacga
      841 gtggtaactt gtaacatgaa aaaagttaca agttacaagt ggtttattta cgaacataaa
      901 ggctaggaaa gcacttgata agtatatcaa gtgctttttt tccgttcgat tttactatcc
      961 aagataccgc ttgacgtacc taatattt
//
